LOCUS       DAVKJJ010000001        77446 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000001 DAVKJJ010000000
VERSION     DAVKJJ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 77446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77446
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.001"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            <1..72
                     /locus_tag="VW144_000001"
     CDS             <1..72
                     /locus_tag="VW144_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9599521.1"
                     /translation="HLEDLSKISEPEKLYAYIAEATA"
     gene            110..415
                     /locus_tag="VW144_000002"
     CDS             110..415
                     /locus_tag="VW144_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9599522.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFCIPAMSAPVFDNIEAAIKEHGLS"
     gene            463..1938
                     /locus_tag="VW144_000003"
     CDS             463..1938
                     /locus_tag="VW144_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEU9599523.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNTAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYATKAK"
     gene            1964..2035
                     /locus_tag="VW144_000004"
                     /pseudo
     CDS             1964..2035
                     /locus_tag="VW144_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            2243..8944
                     /locus_tag="VW144_000005"
     CDS             2243..8944
                     /locus_tag="VW144_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU9599524.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKLEEKAPRIESQASSQEKPLKEDAKAVTNEEMNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLQKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIINTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEIAEEDKAKLAIPGSRIQATGYLEGQPIHATLVVEEGNPAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRAIIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGATGQITLTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRIEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRSNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAIRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTANVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLSVEWEEVPAEN
                     LTKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSASSQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(9192..10041)
                     /locus_tag="VW144_000006"
                     /pseudo
     CDS             complement(9192..10041)
                     /locus_tag="VW144_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10253..10612)
                     /locus_tag="VW144_000007"
     CDS             complement(10253..10612)
                     /locus_tag="VW144_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEU9599525.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYVEMIVELFLMK"
     gene            10704..11660
                     /locus_tag="VW144_000008"
                     /pseudo
     CDS             10704..11660
                     /locus_tag="VW144_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            11644..12201
                     /locus_tag="VW144_000009"
     CDS             11644..12201
                     /locus_tag="VW144_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9599526.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            12194..12448
                     /locus_tag="VW144_000010"
     CDS             12194..12448
                     /locus_tag="VW144_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9599527.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            12460..14514
                     /locus_tag="VW144_000011"
     CDS             12460..14514
                     /locus_tag="VW144_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU9599528.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLIWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHSFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKNKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            14698..15558
                     /locus_tag="VW144_000012"
     CDS             14698..15558
                     /locus_tag="VW144_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU9599529.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            15769..16479
                     /gene="ccdA2"
                     /locus_tag="VW144_000013"
     CDS             15769..16479
                     /gene="ccdA2"
                     /locus_tag="VW144_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9599530.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            16499..17065
                     /locus_tag="VW144_000014"
     CDS             16499..17065
                     /locus_tag="VW144_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEU9599531.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            17076..18188
                     /gene="msrB"
                     /locus_tag="VW144_000015"
     CDS             17076..18188
                     /gene="msrB"
                     /locus_tag="VW144_000015"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9599532.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKTVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            18456..19193
                     /locus_tag="VW144_000016"
     CDS             18456..19193
                     /locus_tag="VW144_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9599533.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            19190..20881
                     /locus_tag="VW144_000017"
     CDS             19190..20881
                     /locus_tag="VW144_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9599534.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIDTGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLNQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            20997..21560
                     /locus_tag="VW144_000018"
     CDS             20997..21560
                     /locus_tag="VW144_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEU9599535.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALTSRPKLIGGARAHYPQDAIRKF"
     gene            21582..27245
                     /locus_tag="VW144_000019"
     CDS             21582..27245
                     /locus_tag="VW144_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9599536.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGTMGLVVLPSAGAVDPVATLALASREGVVEMEGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPAEES
                     KVEQAGEPVAPRKDEQAPVEPENQPEAPEEEKAVEETPKQEESTPDTKAEETVEPKEE
                     TKTAKGTQEEDYVGEALVPEQLPEYKVTEGTLVEESTTELDYKTETTEDPTKYTDEET
                     VLRNGEKGSQVTKTTYKTIGGVKTDQVLSTSTEVTKEPVNQQVIRGTKPIEGTTVETR
                     EEIIPFETKEQEDDTLKRGTRQVTQEGVNDKKQITETYKTIRGEKTSDAPTITETVIE
                     KPQDKIIKNGTKELEKPTLEWAKTDKDVLKKSATASYTLTKPAGVEIKSIKVALKDNT
                     GTVVKEVTVEENNLQATLDNLKYYQGYTLSTTMVYDRGNGEETEILEDKEVQLDLKKV
                     EIKDIKETRLMKVDENGNETDSSLLETVPENLTSYYLKITTNHNKVTRLAITNIEEVT
                     EEETSLFKVTASAPDLIQRDSNNSLSQTYTYYIEKPKASQNNVYYNFKDLVDAMQKNP
                     NGEFKLGSDLNATNVPTPSKSYVTGKFTGHLTSVDGKHFSIHNTARPLFDRVEGGSIK
                     NINLINVNINMPWANQLASLAQTVKNATVENIKVTGTIVGNNNVAGILDKLDDAGIMT
                     NVAFIGKVQSVGDKGWNLAGIASEIYRGNLNKAYADVELKGLNTRAGAIVGHLAHDFN
                     PRDVGAWSNVRNSVAKGTIDVARGDVGGLIGKDWPWGTIDNNISLVNVKRGDIFFGSN
                     DMDDEFVSNWIKPNNATVIGVSSGDKTHKQSSKIKEISQVEADKKIKDMGITADTFEV
                     TEHLVDKLNRRILRDEEYKTIQDYNANRELVYRNVEKLQPFYNKEWIVNQGNKLTTDS
                     PLMNKEVLSVTAMKGNDFITDLTDADHIMVHYADKTKDIFTISPKDSQVKQVKEYSVA
                     ELGEVVYTPNMVVKDRADLISAIEGILSPIELQSDPIYQRLGRTGDNRVNAIKNLYLE
                     ESFKEVKDHLNTFVTKLVENEDHQLNTDEAAKRALIKKIEDNKAAVLLGLTYLNRYYG
                     VKFDNFNIKELMLFKPDFYGKNVSVLDRLIEIGSKEDYIKGSRTQESFREVVAKNTLS
                     GNLHDFLTYNMKLFTNETDINVWFKKAIENNAYVVEQPSTNPAFANKKYRLYEGLNNG
                     QHGRMILSLLNLKDAHLFMISTYNTISFSSFEKYGKDTEEKRETFKSEINKRAKEQVN
                     YLDFWSRLATDNVRDKLLKSQNVVPTPVWDNHGAPGGWPDRFGHRKDKPDYAPIREFF
                     GRIDKYHDYKYGYGAYAYIFADPQPMDAVYFVMSDLISEYGTSAFTHETTHVNDRMAY
                     LGGHRHRQGTDLEAFAQGMLQTPAEHGHQGEYGALGLNMAFERSNDGNQWYNPDPTKL
                     QTRDQIDHYMKNYNEAMMMLDYAEAEAVLPKVKGDNSKWFKKIDRETRRPMDRNKLSG
                     PHQWDKVRDLIDAEKTTNLETIDDLVNNNFMTIHGNPGNKVFHPEDFGTAYVNVNMMA
                     GIYGGNTSQGAPGSLSFKHNAFRMWGYYGYEHGFIDYVSSKHQGAANKENNGLLGDDF
                     IIKKVSNNQFSNLEDWKKQWYGEVLTKSKKGFAPIEINGTQISTYEQLKTMFDEAVTK
                     DLTELNDSSIKDKYRNTVVLKSKVFKALLKNTDGFSGDLFTAPQA"
     gene            27314..29035
                     /gene="pabB"
                     /locus_tag="VW144_000020"
     CDS             27314..29035
                     /gene="pabB"
                     /locus_tag="VW144_000020"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEU9599537.1"
                     /translation="MHRKTVIDFRTLGERYTFTKPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQYLLETGQVEEKVLTLADLAQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            29036..29653
                     /locus_tag="VW144_000021"
     CDS             29036..29653
                     /locus_tag="VW144_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9599538.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALALELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKALMVGVSEELKTLDLTDIAGTCPYPTLLICGIKDKPNL
                     SSMRSLHKLISESQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            29703..30701
                     /locus_tag="VW144_000022"
     CDS             29703..30701
                     /locus_tag="VW144_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9599539.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DLHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQSTAIQINTSSATTSGWVKQDGA
                     WYYFDGNGNLVKNAWQGSYYLKADGKMAQSEWIYDSSYQAWYYLKSDGSYAKNAWQGA
                     YYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDA
                     TYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTA
                     SSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDD"
     gene            30828..31787
                     /locus_tag="VW144_000023"
     CDS             30828..31787
                     /locus_tag="VW144_000023"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEU9599540.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDREKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPIACTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            31884..32723
                     /locus_tag="VW144_000024"
     CDS             31884..32723
                     /locus_tag="VW144_000024"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU9599541.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(32771..32941)
                     /locus_tag="VW144_000025"
     CDS             complement(32771..32941)
                     /locus_tag="VW144_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEU9599542.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            33062..33946
                     /gene="miaA"
                     /locus_tag="VW144_000026"
     CDS             33062..33946
                     /gene="miaA"
                     /locus_tag="VW144_000026"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU9599543.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVSHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVDQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINNRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            33939..35177
                     /gene="hflX"
                     /locus_tag="VW144_000027"
     CDS             33939..35177
                     /gene="hflX"
                     /locus_tag="VW144_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU9599544.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            35170..35793
                     /locus_tag="VW144_000028"
     CDS             35170..35793
                     /locus_tag="VW144_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEU9599545.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKSFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            35808..36737
                     /locus_tag="VW144_000029"
     CDS             35808..36737
                     /locus_tag="VW144_000029"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEU9599546.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNIQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            36758..37513
                     /locus_tag="VW144_000030"
     CDS             36758..37513
                     /locus_tag="VW144_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU9599547.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(37567..38475)
                     /locus_tag="VW144_000031"
     CDS             complement(37567..38475)
                     /locus_tag="VW144_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9599548.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLSHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            38539..38808
                     /locus_tag="VW144_000032"
     CDS             38539..38808
                     /locus_tag="VW144_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEU9599549.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            38808..39188
                     /locus_tag="VW144_000033"
     CDS             38808..39188
                     /locus_tag="VW144_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9599550.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            39277..39504
                     /locus_tag="VW144_000034"
     CDS             39277..39504
                     /locus_tag="VW144_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9599551.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(39544..40269)
                     /locus_tag="VW144_000035"
     CDS             complement(39544..40269)
                     /locus_tag="VW144_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEU9599552.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLKGDILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQTFQKGIVFLDGTIC
                     KPARLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTILKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
     gene            40431..42272
                     /gene="typA"
                     /locus_tag="VW144_000036"
     CDS             40431..42272
                     /gene="typA"
                     /locus_tag="VW144_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU9599553.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQETTMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            42292..42546
                     /locus_tag="VW144_000037"
     CDS             42292..42546
                     /locus_tag="VW144_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEU9599554.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            42910..43209
                     /locus_tag="VW144_000038"
                     /pseudo
     CDS             42910..43209
                     /locus_tag="VW144_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
     gene            43280..45301
                     /locus_tag="VW144_000039"
                     /pseudo
     CDS             43280..45301
                     /locus_tag="VW144_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
     gene            45303..45944
                     /locus_tag="VW144_000040"
     CDS             45303..45944
                     /locus_tag="VW144_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9599555.1"
                     /translation="MIELKNISKKFGSRQLFSDMNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            46048..47400
                     /locus_tag="VW144_000041"
     CDS             46048..47400
                     /locus_tag="VW144_000041"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU9599556.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGEHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            47404..48462
                     /locus_tag="VW144_000042"
     CDS             47404..48462
                     /locus_tag="VW144_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU9599557.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLAKVEHVGA
                     VTKVSDKNTPEPDELVDIQTHFNPKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELRQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            48472..49701
                     /gene="divIB"
                     /locus_tag="VW144_000043"
     CDS             48472..49701
                     /gene="divIB"
                     /locus_tag="VW144_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEU9599558.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEIEE
                     SESKEKEEQDKKLAKKAIKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYAT
                     MKDIRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQF
                     PTKFTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIK
                     AFVSELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSK
                     IKPQLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKRLEEEQKK
                     QEEESNRNQTTQRSSRR"
     gene            49934..50635
                     /gene="pyrF"
                     /locus_tag="VW144_000044"
     CDS             49934..50635
                     /gene="pyrF"
                     /locus_tag="VW144_000044"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU9599559.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            50669..51301
                     /locus_tag="VW144_000045"
     CDS             50669..51301
                     /locus_tag="VW144_000045"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU9599560.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            51481..51884
                     /locus_tag="VW144_000046"
                     /pseudo
     CDS             51481..51884
                     /locus_tag="VW144_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            51980..52744
                     /locus_tag="VW144_000047"
     CDS             51980..52744
                     /locus_tag="VW144_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9599561.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFLAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            52744..53496
                     /locus_tag="VW144_000048"
     CDS             52744..53496
                     /locus_tag="VW144_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9599562.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTAILFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLNNGIIGLLPIFQYMV
                     NSNPYALIYWLALLSIIIPLTVFSVHRNWRRV"
     gene            53506..54204
                     /locus_tag="VW144_000049"
     CDS             53506..54204
                     /locus_tag="VW144_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9599563.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFLEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLSLLCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            54201..54848
                     /locus_tag="VW144_000050"
     CDS             54201..54848
                     /locus_tag="VW144_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9599564.1"
                     /translation="MNEIITLKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(54935..55801)
                     /locus_tag="VW144_000051"
     CDS             complement(54935..55801)
                     /locus_tag="VW144_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9599565.1"
                     /translation="MKLFKPLLTVLVLAFALIFITACSSGGNAGSSSGKTTAKARTID
                     EIKKSGELRIAVFGDKKLFGYVDNDGSYQGYDIELGNQLAQNLGVKVKYISVDAANRA
                     EYLISNKVDIILANFTVTDERKKQVDFALPYMKVSLGVVSPKTGLITDVKQLEGKTLI
                     VTKGTTAETYFEKNHPEIKLQKYDQYSDSYQALLDGRGDAFSTDNTEVLAWALENKGF
                     EVGITSLGDPDTIAAAVQKGNQELLDFINKDIEKLGKENFFHKAYEKTLHPTYGDTAK
                     ADDLVVEGGKVD"
     gene            complement(55813..56570)
                     /locus_tag="VW144_000052"
                     /pseudo
     CDS             complement(55813..56570)
                     /locus_tag="VW144_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            complement(56571..57248)
                     /locus_tag="VW144_000053"
     CDS             complement(56571..57248)
                     /locus_tag="VW144_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9599566.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIEISILSVLLSMMFRTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            complement(57208..57888)
                     /locus_tag="VW144_000054"
     CDS             complement(57208..57888)
                     /locus_tag="VW144_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9599567.1"
                     /translation="MDWSIVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHY
                     RIPVLAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMA
                     ESFRSGLEAISQTQQEIGLAIGLTPLQVFRYVVLPQATAVALPSFSANVIFLIKETSV
                     FSAVALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFG
                     NPSTLSGK"
     gene            58103..58249
                     /locus_tag="VW144_000055"
     CDS             58103..58249
                     /locus_tag="VW144_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEU9599568.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TSFQ"
     gene            58377..59867
                     /gene="lysS"
                     /locus_tag="VW144_000056"
     CDS             58377..59867
                     /gene="lysS"
                     /locus_tag="VW144_000056"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU9599569.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALCEQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKVIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            60491..61627
                     /gene="lctO"
                     /locus_tag="VW144_000057"
     CDS             60491..61627
                     /gene="lctO"
                     /locus_tag="VW144_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEU9599570.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCNVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     regulatory      61879..61967
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            62182..62850
                     /locus_tag="VW144_000058"
     CDS             62182..62850
                     /locus_tag="VW144_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEU9599571.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     gene            complement(63000..63086)
                     /locus_tag="VW144_000059"
                     /pseudo
     CDS             complement(63000..63086)
                     /locus_tag="VW144_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            63191..63958
                     /gene="thiM"
                     /locus_tag="VW144_000060"
     CDS             63191..63958
                     /gene="thiM"
                     /locus_tag="VW144_000060"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9599572.1"
                     /translation="MIKKKAPLVICITNDVVKNFTANGLVALGASPAMSEFPADLEDL
                     LKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVADDLIN
                     NYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVTGEVDV
                     IAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVYNIAGE
                     MAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            63960..64589
                     /locus_tag="VW144_000061"
     CDS             63960..64589
                     /locus_tag="VW144_000061"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9599573.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     regulatory      64798..64886
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            65148..65708
                     /locus_tag="VW144_000062"
     CDS             65148..65708
                     /locus_tag="VW144_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9599574.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     gene            65709..67094
                     /locus_tag="VW144_000063"
     CDS             65709..67094
                     /locus_tag="VW144_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9599575.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILLQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKTYIDYMVELRDGKLREVFQIPS
                     YEMTQVASKEVAASPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGSIAWKGTVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTIKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRTFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            67096..67746
                     /locus_tag="VW144_000064"
     CDS             67096..67746
                     /locus_tag="VW144_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEU9599576.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            67757..68449
                     /gene="tenA"
                     /locus_tag="VW144_000065"
     CDS             67757..68449
                     /gene="tenA"
                     /locus_tag="VW144_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEU9599577.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKASNQEMKRLLKQNAQGLVEGELFIRQQFFKELEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            68454..68978
                     /gene="thiW"
                     /locus_tag="VW144_000066"
     CDS             68454..68978
                     /gene="thiW"
                     /locus_tag="VW144_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEU9599578.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            68978..69781
                     /locus_tag="VW144_000067"
     CDS             68978..69781
                     /locus_tag="VW144_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9599579.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVIKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLVVSKNQVAVLGNGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            69774..70406
                     /locus_tag="VW144_000068"
     CDS             69774..70406
                     /locus_tag="VW144_000068"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9599580.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKIETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(70616..71407)
                     /gene="thiD"
                     /locus_tag="VW144_000069"
     CDS             complement(70616..71407)
                     /gene="thiD"
                     /locus_tag="VW144_000069"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9599581.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDAYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLDSVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     regulatory      complement(71491..71589)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            71773..72168
                     /locus_tag="VW144_000070"
     CDS             71773..72168
                     /locus_tag="VW144_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEU9599582.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     SRLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            72179..72550
                     /locus_tag="VW144_000071"
     CDS             72179..72550
                     /locus_tag="VW144_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU9599583.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHTNLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            72560..74803
                     /locus_tag="VW144_000072"
     CDS             72560..74803
                     /locus_tag="VW144_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9599584.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTTIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFRLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQTLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            75010..76785
                     /gene="spxB"
                     /locus_tag="VW144_000073"
     CDS             75010..76785
                     /gene="spxB"
                     /locus_tag="VW144_000073"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEU9599585.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            76896..77243
                     /locus_tag="VW144_000074"
     CDS             76896..77243
                     /locus_tag="VW144_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9599586.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
BASE COUNT        23461 a        14199 c        17051 g        22735 t
ORIGIN      
        1 catcttgaag acctatcaaa aatttccgaa ccagaaaaac tttatgctta cattgctgaa
       61 gcaaccgctt aatcttgtct attaaaaaat aaaatcggag gaaatccaaa tgattaaaat
      121 tcttgctgcc tgcggtgcag gtgttaactc aagtcaccaa attaaaagtg ctctagaaga
      181 agaactttca aaccgtggtt atgatgttca ctgtgatgca gtcatggtga aagatgtaaa
      241 cgaagacctt atgaaaggtt acgatatctt tacaccaatc gctgcaacag accttggttt
      301 tgaaccaggt atcccagtta tcgaagctgg gccaatctta ttctgtatcc cagcaatgag
      361 cgctccagta tttgacaata ttgaagcagc tattaaagaa cacggattaa gctaattcta
      421 atttgttaac attcatataa cataaaaaag ggaggaactg ttatggatgc aatctttgac
      481 ctaatcggaa aggttttcaa tcccatctta gaaatgggtg gacctgtcat catgttgatc
      541 attttgacag tattggcttt actttttgga gtgaaattct ccaaagcgct tgaaggtggt
      601 atcaaacttg ccatcgctct tacaggtatc ggtgctatca tcggtatgct aaacactgct
      661 ttctcagcat cacttgcaaa attcgttgaa aacactggta tccaattgag tattaccgac
      721 gttggttggg caccactcgc aacaatcact tggggttctg cttggacact atacttcttg
      781 ctcatcatgt tgattgtcaa cgtagtgatg cttgctatga agaaaaccga tacacttgat
      841 gtcgatatct ttgatatctg gcacttgtct atcacaggtc tcttgattaa atggtatgct
      901 gataacaatg gtgtgagtca aggggtttca ctctttattg ctacagcagc tatcgtcctt
      961 gtcggtgtgt tgaaaattat caactctgac ttgatgaaac ctacatttga tgaccttctt
     1021 aacgccccaa gttcatcacc aatgacatca actcacatga actacatgat gaacccagtt
     1081 atcatggttt tggataagat ttttgaaaaa ttcttcccag gccttgataa atatgacttt
     1141 gatgctgcta aattgaacaa gaaaatcggt ttctggggat ctaaattctt catcggtttc
     1201 atccttggta tcgttatcgg tattatggga actccacatc caattgcagg tgttgcagat
     1261 gcagataaat ggcgtcttgt tatcaaagga tggttgtctc ttggtttgac tgccggtgta
     1321 tctttggaac tcttctcact tatcggttca tggttcatcg cagccgtaga accactatca
     1381 caaggtatta caaacgttgc tactaaacgt cttcaaggac gtaaattcaa tatcggtctt
     1441 gactggccat tcatcgctgg tcgtgctgaa atctgggctt gtgccaacgt acttgcacca
     1501 atcatgttga ttgaagcagt gcttctttca aaagttggaa atggtatctt gccacttgca
     1561 ggtatcatcg ctatgggtgt tactccagct ctcttggttg taactcgtgg taaattgctc
     1621 cgtatgatta tcttcggaac actcttgttg ccactcttcc ttctttcagg tacacttatt
     1681 gcaccatttg caacagaact tgctaaaggt gtaggtgcct tcccagaagg tgtgagccaa
     1741 actcaattga ttactcactc tactcttgaa ggaccaatcg aaaaacttct tggttggaca
     1801 attggtaaca ctacaactgg tgatatcaaa gcaatccttg gtgcagtagt cttccttgta
     1861 ttctatatcg gtatctttgc ttggtacaga aaacaaatga tcaaacgtaa cgaagagtac
     1921 gcaacaaaag caaaataatg cgctcctata aaatataaac ttaatgaaaa tcaaagagca
     1981 aactaggaag ctagccgcag gttgctcaaa acacagtttt gaggttgtag ataagactga
     2041 cgaagtcagc tcaaaacatg gttttgaggt tgtagatgaa actgacgaag tcagtaatca
     2101 tatctacggc aaggcgacac tgacgaagtt tgaattggat tttcgcagag tgtaaattgt
     2161 tgaaactagg ttgtcatacc atgtatacca cttgggcttt ggtagtagct aactgcacta
     2221 aatataatat aaggagggtt ttatggggaa aggccattgg aatcggaaaa gagtttatag
     2281 cattcgtaag tttgctgtgg gagcttgctc agtaatgatt gggacttgtg cagttctatt
     2341 aggaggaaat atagctggag aatctgtagt ttatgcggat gaaacactta ttactcatac
     2401 tgctgagaaa cctaaagagg aaaaaatgat agtagaagaa aaggctgata aagctttgga
     2461 aactaaaaat gtagttgaaa ggacagaaca aagtgaacct agttcaactg aggctattgc
     2521 atctgagaag aaagaagatg aagccgtaac tccaaaagag gaaaaagtgt ctgctaaact
     2581 ggaagaaaaa gctccaagga tagaatcaca agcttcaagt caagaaaaac cgctcaagga
     2641 agatgctaaa gctgtaacaa atgaagaaat gaatcaaatg attgaagaca ggaaagtgga
     2701 ttttaatcaa aattggtact ttaaactcaa tgcaaattct aaggaagcca ttaaacctga
     2761 tgcagacgta tctacgtgga aaaaattaga tttaccgtat gactggagta tctttaacga
     2821 tttcgatcat gaatctcctg cacaaaatga aggtggacag ctcaacggtg gggaagcttg
     2881 gtatcgcaag actttcaaac tagatgaaaa agacctcaag aaaaatgttc gccttacttt
     2941 tgatggcgtc tacatggatt ctcaagttta tgtcaatggt cagttagtgg ggcattatcc
     3001 aaatggttat aaccagttct catatgatat caccaaatac cttcaaaaag atggtcgtga
     3061 gaatgtgatt gctgtccatg cagtcaacaa acagccaagt agccgttggt attcaggaag
     3121 tggtatctat cgtgatgtga ctttacaagt gacagataag gtgcatgttg agaaaaatgg
     3181 gacaactatt ttaacaccaa aacttgaaga acaacaacat ggcaaggttg aaactcatgt
     3241 gaccagcaaa atcatcaata cggacgacaa agaccatgaa cttgtagccg aatatcaaat
     3301 cgttgaacga ggtggtcatg ctgtaacagg cttagttcgt acagcgagtc gtaccttaaa
     3361 agcacatgaa tcaacaagcc tagatgcgat tttagaagtt gaaagaccaa aactctggac
     3421 cgttttaaat gacaaacctg ccttgtacga attgattacg cgtgtttacc gtgacggtca
     3481 attggttgat gctaagaagg atttgtttgg ttaccgttac tatcactgga ctccaaatga
     3541 aggtttctct ttgaatggtg aacgtattaa attccatgga gtatccttgc accacgacca
     3601 tggggcgctt ggagcagaag aaaactataa agcagaatat cgccgtctca aacaaatgaa
     3661 ggagatggga gttaactcca tccgtacaac ccacaaccct gctagtgagc aaaccttgca
     3721 aatcgcagca gaactaggtt tactcgttca ggaagaggct tttgatactt ggtatggtgg
     3781 caagaaacct tatgactatg gacgtttttt tgaaaaagat gccactcacc cagaagctcg
     3841 aaaaggtgaa aaatggtctg attttgacct acgtaccatg gtcgaaagag gcaaaaacaa
     3901 ccctgctatc ttcatgtggt caattggtaa tgaaataggt gaagctaatg gtgatgccca
     3961 ctctttagca actgttaaac gtttggtcaa ggttatcaag gatgttgata agactcgcta
     4021 tgttaccatg ggagcagata aattccgttt cggtaatggt agcggagggc atgagaaaat
     4081 tgctgatgaa ctcgatgctg ttggatttaa ctattctgaa gataattaca aagcccttag
     4141 agctaagcat ccaaaatggt tgatttatgg atcagaaaca tcttcagcta cccgtacacg
     4201 tggaagttac tatcgccctg aacgtgaatt gaaacatagc aatggacctg agcgtaatta
     4261 tgaacagtct gactatggga atgatcgtgt gggttggggg aaaacagcaa ccgcttcatg
     4321 gacttttgac cgtgacaacg ctggctatgc tggacagttt atctggacag gtacggatta
     4381 tattggtgaa cctacaccat ggcacaacca aaatcaaact cctgttaaga gctcttactt
     4441 tggtatcgta gatacagccg gcattccaaa acatgacttc tatctctacc aaagccaatg
     4501 ggtttctgtt aagaagaaac cgatggtaca ccttcttcct cactggaact gggaaaacaa
     4561 agaattagca tccaaagtag ctgactcaga aggtaagatt ccagttcgtg cttattcgaa
     4621 tgcttctagt gtagaattgt tcttgaatgg aaaatctctt ggtcttaaga ctttcaataa
     4681 aaaacaaacc agcgatgggc ggacttacca agaaggtgca aatgctaatg aactttatct
     4741 tgaatggaaa gttgcctatc aaccaggtac cttggaagca attgctcgtg atgaatctgg
     4801 caaggaaatt gctcgagata agattacgac tgctggtaag ccagcggcag ttcgtcttat
     4861 taaggaagac catgcgattg cagcagatgg aaaagacttg acttacatct actatgaaat
     4921 tgttgacagc caggggaatg tggttccaac tgctaataat ctggttcgct tccaattgca
     4981 tggccaaggt caactggtcg gtgtagataa cggagaacaa gccagccgtg aacgctataa
     5041 ggcgcaagca gatggttctt ggattcgtaa agcatttaat ggtaaaggtg ttgccattgt
     5101 caaatcaact gaacaagcag ggaaattcac ccttactgcc cactctgatc tcttgaaatc
     5161 gaaccaagtc actgtcttta ctggtaagaa agaaggacaa gaaaagactg ttttggggac
     5221 agaggtgcca aaagtacaga ccattattgg agaggcacct gaaatgccta ccactgttcc
     5281 gtttgtatac agtgatggta gccgtgcaga acgtcctgta acctggtctt cagtagatgt
     5341 gagcaagcct ggtattgtaa cggtgaaagg tatggctgac ggacgagaag tagaagctcg
     5401 tgtagaagtg attgctctta aatcagagct accagttgtg aaacgtattg ctccaaatac
     5461 tgacttgaat tctgtagaca aatctgtttc ctatgttttg actgatggaa gtgtacaaga
     5521 gtacgaagta gatagctggg agattgccga agaagataaa gctaagttag caattccagg
     5581 ttctcgtatt caagcgaccg gttatttaga aggtcaacca attcatgcaa cccttgtggt
     5641 agaagaaggc aatcctgcag cacctgtagt gccaactgtt actgttggag gtgaagcagt
     5701 aacaggtctt actagtcgac aaccaatgca atatcgtact ctatcttatg gtgcccaatt
     5761 gccagaagtc acagcaagtg ctgaaaatgc tgatgtgaca gttcttcaag caagcgcagc
     5821 aaacggcatg cgtgcgatca tctttattca gcctaaagat ggtggccctc ttcaaaccta
     5881 tgcaattcaa ttccttgaag aagcgccaaa aattgctcac ttgagcttgc aagtggaaaa
     5941 agctgacagt ctcaaagaag accaaactgt caaattgtcg gttcgagctc actatcaaga
     6001 tggaacgcaa gctgtattac cagctgataa agtaaccttc tctacaagtg gtgaagggga
     6061 agtcgcaatt cgtaaaggaa tgcttgagtt gcataagcca ggagcagtca ctctcaaagc
     6121 ggaatatgag ggagctacag gtcaaatcac tctcactatc caagccaata ctgagaagaa
     6181 gattgcgcaa tctatccgtc ctgtaaatgt agtcacagat ttgcatcaaa aacctactct
     6241 tccaacaaca gtaacggttg agtatgacaa aggtttccct aaagctcaca aagtcacttg
     6301 gcaagctatt ccgaaagaaa aactagactc ttatcaaaca tttgaagtac taggtaaagt
     6361 tgaaggaatt gaccttgaag cgcgtgcaaa agtctctgta gaaggtatcg tttcagttga
     6421 agaagtcagt gtgacaactc caatcgcaga agcaccacaa ttaccagaaa gcgttcggac
     6481 atatgattca aatggtcacg tttcatcagc taaggttgca tgggatgcga ttcgtataga
     6541 gcaatacgct aaggaaggtg tctttacagt taatggtcgc ttagaaggta ctcaattaac
     6601 aactaaactt catgttcgcg tatctgctca aactgagcaa ggtgcaaaca tttctgacca
     6661 atggaccggt tcagaattgc cacttgcctt tgcttcagat tcaaatccaa gcgacccagt
     6721 ttcaaatgtt aatgacaagc tcatttccta caataaccaa ccagccaatc gttggacaaa
     6781 ctggaatcgt agtaatccag aagcttcagt cggtgttctg tttggagatt caggtatctt
     6841 gagcaaacgc tccgttgata atctaagtgt cggattccac gaagaccatg gagttggtgc
     6901 accgaagtct tatgtgattg agtattatgt tggtaagact gtcccaacag ctcctaaaaa
     6961 ccctagtttt gttggtaatg aggaccatgt ctttaatgat tctgccaact ggaaaccagt
     7021 tactaatcta aaagcccctg ctcaactcaa ggctggagaa atgaaccact ttagctttga
     7081 taaagttgaa acctatgcta ttcgtattcg catggttaaa gcagataaca agcgtggaac
     7141 gtctatcaca gaggtacaaa tctttgcgaa acaagttgcg gcagccaaac aaggacaaac
     7201 aagaatccaa gttgacggca aagacttagc aaacttcaac cctgatttga cagactacta
     7261 ccttgagtct gtagatggaa aagttccggc agtcacagca aatgttagca acaatggtct
     7321 cgctaccgtc gttccaagcg ttcgtgaagg tgagccagtt cgtgtcatcg cgaaagctga
     7381 aaatggcgac atcttaggag aataccgtct gcacttcact aaggataaga acttactttc
     7441 tcataaacca gttgctgcgg ttaaacaagc tcgcttgcta caagtaggtc aagcacttga
     7501 attgccgact aaggttccag tttacttcac aggtaaagac ggctacgaaa caaaagattt
     7561 gtcagttgaa tgggaagaag ttccagcaga aaatctgaca aaagcaggtc aatttaccgt
     7621 tcgaggccat gtccttggta gtgatcttgt tgctgaggtc actgtacgag tgacagacaa
     7681 actaggcgaa gctctttcag ataaccctaa ctatgatgaa aacagtaacc aggcctttgc
     7741 ttcagcaacc aatgatattg acaaaaactc tcatgaccgc gttgactatc tcaatgacgg
     7801 agatcattca gaaaatcgtc gttggacaaa ctggtctcca acaccatctt ctaatccaga
     7861 agtatcagcg ggtgtgatct tccgtgaaaa tggtaagatt gtagaacgga ctgttgcgca
     7921 agccaaactt cacttctttg cagatagtgg tacggatgca ccaactaaac tcgttttaga
     7981 acgctatgtc ggtccagagt ttgaagtgcc aacctactat tcaaactacc aagcctacga
     8041 cgcagaccat ccattcaaca atccagaaaa ttgggaagct gtgccttatc gtgcggataa
     8101 agacatcgca gctggtgatg aaatcaacgt aacatttaaa gctgtcaaag ccaaagctat
     8161 gagatggcgt atggagcgta aagcagataa gagcggtgtt gcgatgattg agatgacctt
     8221 ccttgcacca agtgaattgc ctcaagaaag cacgcaatcg aagattcttg tagatggaaa
     8281 agaacttgct gatttcgctg aaaatcgtca agactatcaa attacctata aaggtcaacg
     8341 gccaaaagtc tcagttgaag aaaacaatca agtagcttca actgtggtag atagtggaga
     8401 agatagcctt ccagtacttg ttcgcctcgt ttcagaaagt ggaaaacaag tcaaggaata
     8461 ccgtatccac ttgactaagg aaaaaccagt ttctgagaag acagttgctg ctgtacaaga
     8521 agatcttcca aaactcgaat ttgttgaaaa agatttggcc tacaagacag ttgagaaaaa
     8581 agattcaaca ctgtatctag gtgaaactcg tgtagaacaa gaaggaaaaa ctggtaaaga
     8641 acgtatcttt acagcgatta atcctgatgg aagtaaggaa gaaaaactcc gtgaagtggt
     8701 agaagctccg acagaccgca tcgtcttggt tggaaccaaa ccagtagctc aagaagctaa
     8761 aaaaccacaa gtgtcagaaa aagcagatac aaaaccaatt gattcaagtg aagctagtca
     8821 aactaataaa gcccagttac caaatacagg tagtgcgtca agccaagcag cagtagcagc
     8881 aggtttagct cttctaggtt tgagtgcagg attagtagtt actaaaggta aaaaagaaga
     8941 ctagaaattt tgataccttc tttatcatta aagaccaaag aaaaactctt gtcctaaata
     9001 gaagataaag agtctggaac aaaatagttc tcaaccacaa aaaagctaga tatttccaat
     9061 tgtggaactc tagcttttta attttgagtc attctcttat tgtatagtgg attgaaacta
     9121 gaatagtaca cttctacttc taaaacattg ttagaaatcg atttgactgt ccttatcgat
     9181 ttgtcctgtt cttatttcat tttactatat tttaggaggt tattggccat aagtaccaat
     9241 cccatgtcaa ttctcacttg cccctcagat tacatctctt gtaacccaaa gaagccttta
     9301 tctgcccaaa gacaggttcc acatcaatct tgcgttgagc gaaaatctgt ctaccttggg
     9361 gagataaaag cgcctgacat tctttagctt tcaagttttg atagcgttcg ttcatatata
     9421 gtcccttttg aggggctgat tcaggttcgt cggcgtagta aaccttgatt tcctgttgaa
     9481 agtctgtctg tgttttctga tgtttggtat ggtgaaaacg ataataccag ccatcaggat
     9541 gtgtgtagct atcctccttg tcattatagt gccaattcgc taagttttta gctgactctt
     9601 tataccctct cttttgtttc ttatcaaaca tggcatattt aatcaggagg ggagtgattt
     9661 cttcttgaaa atagacttgt atctgttctt gaagtttggc ggagaatttc tctgtcgttt
     9721 tcttccacac aaaactatac ttgttggcat tggcttcaat cttagttccg tcaataaaca
     9781 aacaatctaa ggtcactaac tcttccattt ttcaattttt cgaccagaga aaatcccttg
     9841 tgaataggca aatagaagag tagatacaag cattttaagg tgataagaca ggcgatcaaa
     9901 ggcatgatag aaggcgtgga aatgacaatc ctccaaggta ttcaccactt tttcaatagt
     9961 aaaaacgaga tggtcttgtg gcaagaagga actgatttct agtggtaaag ttgtttgatt
    10021 tgtgttatag tgaatatgca tgggatagcc tttctaaatg gtttattggg caattctatt
    10081 ttaccaagac tatcgtaacc atgcaaaaaa agcaacggca aatccgttgc tttttttgga
    10141 gataagagac tattgtccca gactcttttc tttgtataaa attgtatagt gttattgtct
    10201 atactatttt gtagagaaag aaaaaatccc tagaaaatgc ttagggattc gactatttca
    10261 ttaagaacaa ttcaacaatc atttctacat acatgataag ggttaataag aatcctgcac
    10321 gccagaagaa tttgatgaat ttaggatagt aaaagctgcg ttttttcaaa aggaagaaaa
    10381 agacgagaat aatggctagg atcgagaggg ctagccccag tctagggagg aaattatggg
    10441 taaaggtttt agctgtaatc aggtaatatt caaataccaa gaccggaaaa gccaaatccg
    10501 caaagtttcg tcctagtttt tttaatctaa aaagtttggt taggataatg cagacgacta
    10561 aggtcagtat caataataaa atagatgcta atttcattaa aatcataccc atattgtatc
    10621 ataaaaaaag gctaatggaa acgagaaaca gaggaattta taggaaagtc aggcttttga
    10681 gattgtgggt gatttttgtt ataatgaaag tcatgaaatc ttataatacc ttgaatgatt
    10741 attatcgaaa attctttgga gaaaagactt ttaaagtccc tattgatgcg ggatttgact
    10801 gtcccaatcg tgatggaact gtggctcatg gaggctgtac tttttgtacg gtttcaggtt
    10861 ctggagatgc tattgtagcc ccagatgcgc ctatccgtga gcaattttat aaggaaattg
    10921 actttatgca ccgtaagtgg ccggatgttc agaagtatct ggtttatttt caaaatttta
    10981 ccaacaccca tgaaaaggtg gaagttattc gagagcgtta tgaacaggct atcaataagc
    11041 caggtgtggt aggtatcaat attggaacgc gcccagactg tttaccagac gaaaccatcg
    11101 aatatttggc tgagttatcg gagtgcatgc atgtgacggt tgaattgggc ttgcagacca
    11161 cttatgaagc aacctctgac ctgattaacc gtgtgcattc ttatgaattg taggtggaaa
    11221 cggtcaagcg tttgagaaag tatcccaaga ttgagattgt ttcccatttg atcaatggct
    11281 tgcctggtga aacccatgag atgatggttg aaaatgtccg ccgttgtgtc acggataacg
    11341 atattcaagg gattaaactg catttgctcc atcttatgac aaatactcgt atgcaacgag
    11401 attaccatga gggacgttta caattgatga gtcaggacga atatgtcagg gtcatctgtg
    11461 accaactgga aattattccc aaacatatcg tcatccatcg aattacagga gatgcgccta
    11521 gggatatgct gattggtcct atgtggagcc tcaaaaaatg ggaagtcctc aacagcattg
    11581 agatggagat gcgacgtcgt ggaagtgttc aaggatgcaa ggctgtaaaa caggagtttg
    11641 aaaatgaaaa gaccacttga gatggcacat gattttttgg ctgaggtcgt gacaaaagag
    11701 gatgtcgtag tggatgcgac tatgggaaat ggtcatgaca cgcttttttt agccaagcta
    11761 gccaagcaag tctatgcctt tgatattcag aagcaagcct tggaaaagac ccaagagcgt
    11821 ttgcatcagg ctggcttgac aaatgcccag ttaatcttgc aaggccatga gacactggac
    11881 cagtttgtga caaaagctaa ggcagggatt tttaatctgg gctatttgcc gtcagctgat
    11941 aagtctgtca tcacccgacc gcagacaacg attgaggcat tagaaaagct atgtggctta
    12001 cttgtcaaag gtggacgaat tgctattatg atttactatg gtcatgaagg aggcgacctc
    12061 gagagagatg ctgtcttgga ttttgtgatc cagttgaacc aacaagagta cacagctgcc
    12121 atttaccgaa ctttaaacca agtcaacaac ccgccgtttt tagtgatgat tgaaaaatta
    12181 gagagataca gacatggata aacaatacct acacgaaaag ctggatgcca tgcgccagaa
    12241 ttttgttgaa tcaactcacc acgaacgagc ggtgggcgtg ctagaccaag cgcatatgag
    12301 caaaaaaatg cttaaaatca agaaaaaatt agttgctctt gaaatggaac ggtgccagag
    12361 aaaaattgag cacaaagact gttccaagat tgaccaaaaa atcaaagagc agaaggagat
    12421 atttgaatcc tgttgtaaaa aagattaagg aggacgtgcg tggaattact gatttacctc
    12481 atcctatttt tactggtttt gattgtctca agtacaacca ataagctcct accctttttg
    12541 cctctccctt tggtgcagat tcttttggga attgtgattg gtctcttttt acccaatact
    12601 gactttcatc ttaatacgga gttgtttttg gccctggtta tcggaccctt gcttttccga
    12661 gaggctgaag aagcagatgt tacggctatt ttaaaacact ggcgaatcat tgtttatctc
    12721 atatttccag tgatttttat ctcgaccctg agtttgggtg gcttggccca tcttatttgg
    12781 ttcagccttc ccttggcagc ttgcttggct gttggggcag cccttggtcc tacggacttg
    12841 gtggcctttg cctctctttc ggagcgtttt agctttccta agcgcgtgtc caatattctt
    12901 aagggcgaag gactcttgaa tgatgcttct ggtttggtgg cttttcaggt agctttgaca
    12961 gcttggacaa ctggagcttt ttctctgggg caagctagca gttcgctcat cttttcaatc
    13021 ctaggcggtt ttttaattgg ctttttaaca gccatgacca atcgtttcct ccatagcttc
    13081 ttgctaagtg tgagagcaac ggatattgct agtgagctcc ttctggaatt gagtttgccc
    13141 ttggtaacct tcttcctggc agaagaagtc catgtttcag ggattattgc cgtcgtagtt
    13201 gctggaattt taaaggcaag tcgcttcaag aaaatcacgc tcctcgaagc ccaagtggat
    13261 acggtgaccg agacggtctg gcatacagtg acctttatgc tcaacggttc tgtctttgtg
    13321 attttaggga tggagttgga aatgatagca gaacctatct tgaccaatcc aatctataat
    13381 cctctacttt tattgctatc tctcatcgcc cttacctttg tcctctttgt cattcgtttt
    13441 attatgatct atggctatta tgcctataga acccgacgcc tcaagaaaaa gctaaataag
    13501 tatatgaagg acatgtttct cttgaccttt tcaggtgtta agggaacggt gtcgattgct
    13561 acgattctct tgataccaag taatctagaa caggagtatc ctctcttgct tttccttgtt
    13621 gcaggtgtga cgcttgtcag ctttttaaca ggtctcttgg tcttgcctca tctttctgat
    13681 gaagaggaag aaagcaagga ttatctcatg catatcgcca ttttgaatga agtaacgcta
    13741 gagttggaaa aagagttgga agacactaga aataaacttc ccctctatgc ggctattgac
    13801 aattatcatg gacgtattga aaatctcatt ttaagccaag aaaaccagga tgatcaagaa
    13861 gactgggctg ctttgaaact cttgattctt agtattgaaa gtgatggttt ggaacaggcc
    13921 tatgaagagg ggaacattag caatcgtgct taccgagttt accaacgtta tctgaaaaat
    13981 atagaacaag gaatcaatcg taaacttgcc tcaagactga cctattattt tcttgtttcc
    14041 ttgaggattt tacgttttct ccttcatgaa gtttttactc tcggaaagac cttccgtagc
    14101 tggaagaaca aggagcaaag ccgtctccgt gctcttgatt atgaccaaat tgcagagctc
    14161 tatcttgcca atacagagat gattattgaa agtttggaaa acctgaaggg agtctacaga
    14221 cgctctttga ttagttttat gcaggagtct cgtcttcgag aaacagctat tatcagcagt
    14281 ggtgcctttg tcgaacgggt tatcaatcgt gtcaaaccca acaatatcga tgaaatgctg
    14341 agaggctatt atctggagcg caagttgatt ttcgaatacg aagaaaaacg attgattacg
    14401 actaagtatg ccaagaaatt acgacaaaat gtaaataact tagagaacta ttccttgaag
    14461 gaagctgcca ataccctacc gtatgatatg gtggaattgg taagaagaaa ttagttaata
    14521 ctcttcgaaa atctcttcaa accacgtcag cgtcgcctta ccgtatgtat gtgactgact
    14581 tcgtcagtct tatctacaac ctcaaaacag tgttttgagc agcttgcggc tagctttcta
    14641 gtttgctctt tgattttcat tgagtataag attataagtg aaggagtatg atatgacatg
    14701 aaaaagtggt ggaaagagct gatagacaaa cctttattaa aagctttttt gcattattat
    14761 caagcatcag atagtgagtt gaccagtgtc gcagtagcct actattggtt gatttcgatt
    14821 ttccccctgc ttttggtggt ggtcaatatc ctcccctatt ttcagattcc tgtgggagaa
    14881 tttttgggct ttatgaaaga cgttctgccc ccaagcctct atgaaggtgt ggaaaaaata
    14941 gccagagaag ttttgacgca accttcaaca ggtttattga gtttttctgt tttatcggcg
    15001 ttatggagtt tttcaaaatc tatgaatttc ttgcaaaaag cttttaacaa ggcttatggc
    15061 gtcgaaaaga gtcgtggact tatttcccat cagatgctga gtctcctagt cagttttggc
    15121 ttgcagctcc tctttgcgtt tgccttgttt ttgatcttat ttggtcagat gattttggct
    15181 ttacttgctc attattggac aaaagatggc attatctatc aggctttaca agggttggca
    15241 ggtcctctga tttacgccct cctctttgct atcttggtca tgctttatta ttttcttccc
    15301 aatctatcta acaggaaaat tagctatacc ctaccaggca gtgcctttgt tctcttagtg
    15361 attttaggtt tactaactct gttctctagt tatctcaatt actatgtcca tcatttggtg
    15421 gatgtccgaa ttttaggttc cgttcttctt gttgttatga tgttttggtt tatcttgatt
    15481 gctaaaattg ttatcctagg cgcggttatc aatgccagta tgcagagttt gaaagatccg
    15541 gtctttaaaa aagactaact atttatctat tataaaagcg catagtatca agactttata
    15601 gaaccttgat actatgcgct tttgtgcttt gacagttttt tgtatagttt ttgtccaaga
    15661 tttctaaatc atctgattaa attcctattt tcccctatca ttgtccctgt tttttcgaaa
    15721 atggaaggag tataataaat ctatcaataa aatgatagga ggaactttat gggtcatatt
    15781 ttcttttttc taagtgtctt tttggcaggg attctatcct tcttttctcc ttgtatctta
    15841 cctttgttac cggtctatac aggggtgtta ctagatgata aggatggtgc tcaggcttct
    15901 agcggcaaat tttcaatctc agttactagt ttattacgaa ctctggcctt tatagcagga
    15961 atttccttta tatttatttt gttgggctat ggagctggtt ttttaggcga tttgctttat
    16021 gcttcttggt tccaatatct tactggggca attattatcc ttcttggttt gcaccaaatg
    16081 gagattctac actttaaggg gctttataag gaaaagaggc tacaactgca aggacagggg
    16141 caaaatggta agggctatag tcaggcattt ttattgggct tgacctttag ttttgcttgg
    16201 acgccttgcg tggggccggt tctggggtct gttttggcct tggcggcttc aggtggttca
    16261 ggagcttggc agggagctgg tctcatgttg gtgtatacgc tgggcttggc gctaccattc
    16321 ttgcttctag ctctgacctc tagttatgtt ttgaaacatt tccgaaaact tcatccctat
    16381 ctcggaatcc tcaaaaaagt gggaggtttt ctcattattg tgatgggact cttggttctg
    16441 tttggaaatg cttcaatttt aagtcaatta tttgaataaa atggaaagga atatcaatat
    16501 gaaaaaatgg caaacatgtg ttcttggagc gggttcgctc ctttgtttga cggcttgttc
    16561 aggcaagtcc gtgactagtg aacaccaaac gaaagatgaa atgaagacgg agcagacagc
    16621 tagtaaaaca agcgcagcta aagggaaaga ggtggctgat tttgaattga tgggagtaga
    16681 tggcaagacc taccgtttat ctgattacaa gggcaagaaa gtctatctca aattctgggc
    16741 ttcttggtgt tccatctgtc tggctagtct tccagatacg gatgagattg ctaaagaagc
    16801 tggtgatgac tatgtggtct tgacagtagt gtcaccagga cataaggggg agcaatctga
    16861 agcggacttt aagaattggt ataagggatt ggattataaa aatctcccag tcctagttga
    16921 cccatcaggc aaacttttgg aaacttatgg tgtccgttct tacccaaccc aagcctttat
    16981 agacaaagaa ggcaagctgg tcaaaacaca tccaggattc atggaaaaag atgcaatttt
    17041 gcaaactttg aaggaattag cctaggaggt gtcttatgaa tgataagtta aaaatcttct
    17101 tgttgctagg agtatttttt ctagccataa ccggtttcta tgttctattg atacgaaatg
    17161 cagggcagac agatgcctcg caaattgaaa aggcggcagt tagccaagga ggaaaaacag
    17221 tgaaaaaaac agaaattagt aaagacgcag acttgcacga aatttatcta gctggaggtt
    17281 gtttctgggg agtggaggaa tatttctcac gcgttcccgg ggtgacggat gccgtttcag
    17341 gctatgcaaa tggtagagga gaaacaacca agtacgaatt gattaaccaa acaggtcatg
    17401 cagaaaccgt ccatgtcacc tatgatgcca agcaaatttc tctcaaggaa atcctgcttc
    17461 actatttccg cattatcaat ccaaccagca aaaataaaca aggaaatgat gtggggaccc
    17521 agtaccgtac tggtgtttat tacacagatg acaaggattt ggaagtgatt aaccaagtct
    17581 ttgatgaggt ggctaagaaa tacgatcaac ctctagcagt tgaaaaggaa aacttgaaga
    17641 attttgtggt ggctgaggat taccatcaag actatctcaa gaaaaatcca aatggctact
    17701 gccatatcaa tgttaatcag gcggcctatc ctgtcattga tgccagcaaa tatccaaaac
    17761 caagtgatga ggaattgaaa aagaccctgt cacctgagga gtatgcagtt acccaggaaa
    17821 atcaaacaga acgagctttc tcaaaccgtt actgggataa atttgaatcc ggtatctatg
    17881 tggatatagc aactggggaa cctctctttt catcaaaaga caaatttgag tctggttgtg
    17941 gctggcctag ttttacccaa cccatcagtc cagatgttgt cacctacaag gaagataagt
    18001 cctacaatat gacgcgtatg gaagtgcgga gccgagtagg agattctcac cttgggcatg
    18061 tctttacgga tggtccacag gacaagggcg gcttacgtta ctgtatcaat agcctctcta
    18121 tccgctttat tcccaaagac caaatggaag aaaaaggcta cgcttattta ctagattatg
    18181 ttgattaata ctcttcgaaa atctcttcaa accacgtcag cgtcgcctta ccgtatgtag
    18241 ggttactgac ttcgtcagtt ttatctacaa cctcaaagct atactttgag ctgacttcgt
    18301 cagttttatc tacaacctca aagcggtact ttgagaaacc tgtggctatc ttcctagttt
    18361 actttttgat ttttattgag tataagaggt ctttcctaag cagttagagg agaattttgc
    18421 tatactgata ctagtaaatg gtaaaggagc agagcatgac ctacacaatc ttaatcgtag
    18481 aagatgaata tctggtaaga caaggtttga ctaaactggt caatgtagca gcctacgata
    18541 tggaaatcat cggtcaggct gaaaatggaa ggcaggcttg ggaattgatc caaaagcagg
    18601 ttccagatat cattttaacc gatatcaaca tgcctcatct aaatggcatc cagttggcca
    18661 gtttggtacg agaaacctat cctcaggttc atttggtctt tttaacaggt tacgatgatt
    18721 ttgattatgc cttgtctgct gtcaaactag gtgtggacga ctacctgctc aaaccctttt
    18781 ctcgtcagga tattgaggaa atgttgggga aaatcaaaca aaaactagac aaggaagaga
    18841 aagaagagca gttacaagat ttattgacta ataggtttga aggaaacatg gcccagaaaa
    18901 tccagtctca tctggctgat agccaattta gtttaaagtc tttagccagt gacttaggtt
    18961 ttagtccgac ctatctgagt tctttgatta agaaagagtt gggcttgcct tttcaggatt
    19021 atctggtgag agaacgtgtt aaacaagcca agctcttgct tttaactaca gatctgaaga
    19081 tttatgagat cgcagagaag gttggttttg aagatatgaa ctattttacc caacgtttta
    19141 agcagattgc aggtgtgaca cctcgtcagt ttaagaaggg agaagaccga tgaagcgttc
    19201 ttctctttta gttagaatgg ttatttccat ctttctggtc tttctcattc tcctagctct
    19261 ggttggaact ttctactatc aatcaagttc ttcagccatt gaggccacca ttgagggtaa
    19321 cagccaaacg accatcagcc agactagcca ctttattcag tcttatatca aaaaactaga
    19381 aaccacttcg accggtttga cccagcagac ggatgttctg gcctatgctg agaatcccag
    19441 tcaagacaag gtcgagggaa tccgagattt gtttttgacc atcttgaagt cagataagga
    19501 cttgaaaact gttgtgctgg tgaccaaatc tggtcaggtc atttctacag atgacagtgt
    19561 gcagatgaaa acttcctctg atatgatggc tgaggattgg taccaaaagg ccattcatca
    19621 gggagctatg cctgttttga ctccagctcg taaatcagat agtcagtggg tcatttctgt
    19681 cactcaagaa cttgttgatg caaagggagc caatcttggt gtgcttcgtt tggatatttc
    19741 ttatgaaact ctggaagcct atctcaatca actccagttg gggcagcagg gctttgcctt
    19801 cattatcaat gaaaaccatg aatttgtcta ccatcctcaa cacacagttt atagttcgtc
    19861 tagcaaaatg gaggctatga aaccctacat cgatacaggt cagggttata ctcctggtca
    19921 caaatcctac gtcagtcaag agaagattgc aggaactgat tggacggtac ttggcgtgtc
    19981 atcattggaa aagttagacc aggttcggag tcagctcttg tggaccttgc ttggggccag
    20041 tgtcacatct cttcttgtct gtctctgctt agtgtggttc agtcttaaac gctggattgc
    20101 tcctttgaag gatttgagag aaaccatgtt ggaaattgct tctggtgctc aaaatcttcg
    20161 tgccaaggaa gttggtgcct atgaactgag agaagtaact cgccaattta atgccatgtt
    20221 gaatcagatt gatcagttga tggtagctat tcgtagccag gaagaaacga cccgtcagta
    20281 ccaacttcaa gccctttcga gccagattaa tccacatttc ctctataaca ctttggacac
    20341 catcatctgg atggctgaat ttcatgatag tcagcgagtg gtgcaggtga ccaagtcctt
    20401 ggcaacctat ttccgcttgg cgctcaatca aggcaaggac ttgatttgtc tctctgacga
    20461 aatcaatcat gtccgccagt atctctttat ccagaaacaa cgctatggag ataagctgga
    20521 atacgaaatt aatgaaaatg ttgcctttga taatttagtc ttacccaagc tggtcctaca
    20581 accccttgta gaaaatgctc tttaccatgg cattaaggaa aaggaaggtc agggccatat
    20641 taaactttct gtccagaaac aggattcggg attggtcatc cgtattgagg atgatggcgt
    20701 tggcttccaa gatgctggtg atagtagtca aagtcaactc aaacgtgggg gagttggtct
    20761 tcaaaatgtc gatcaacggc tcaaacttca ttttggagcc aattaccaga tgaagattga
    20821 ttctagaccc caaaaaggaa cgaaagttga aatatatata aatagaatag aaactagcta
    20881 actcccagtc aattctggga gttttatgcg taattgggca aactttctag gttgtctatc
    20941 ttgctaaaaa caagaaaaaa cggtatgata gaagagtgac aacagaggta acagagatga
    21001 acgagacaca gattcaaaga gaaacacgtc aggtagtgga agatgtttta gaaaagacca
    21061 atttgaaaca gggggccctt tttgttttag gtctatcttc tagtgaggtg ctaggtggtc
    21121 agattgggaa agaatccagt caagagattg gtgagctgat agtggagact atcttgggta
    21181 ttctaggtag tagaggtatc catttagcag ttcaaggttg tgagcatgtc aatcgggctc
    21241 tcgttgttga acgtcaggtg gcagagcagt ttggtctgga aattgtcagt gtccatccta
    21301 ctcttcatgc aggaggttcg gggcagttgg cagcctttaa gtttatgcag gatccagttg
    21361 aggttgaatt tatcaaggct catgctggat tggatatcgg agacactgca attggcatgc
    21421 atgtcaagca tgttcaggtt ccgattcgcc ctattttgag agagattggt catgcccatg
    21481 taacggcact gactagtcgt ccaaaattaa tcggaggtgc gcgtgcgcac tatccgcaag
    21541 atgctattag gaagttttga gaatgaataa aggagataaa agtgtttaaa aaagaccgtt
    21601 tttcaattcg taagattaag ggagttgtag gctctgtatt tcttggaagc cttttgatgg
    21661 ctccttctgt agtggacgca gccacctatc actatgtaaa taaagagatt atttcacaag
    21721 aagctaaaga tttaattcag acaggaaagc ctgacaggaa tgaagttgta tatggtttgg
    21781 tgtatcaaaa agatcagttg cctcaaacag ggacagaagc atctgttttg acagcttttg
    21841 gtttgctgac tgttgggagc ttgcttttaa tctacaagag aaagaaaatt gctagcgtct
    21901 ttctagttgg aactatggga ttggtagttc ttcctagtgc aggggctgta gacccagttg
    21961 cgaccctagc actggctagt cgagagggtg ttgttgaaat ggagggctat cgctatgttg
    22021 gttatctatc aggtgacatc ctcaaaacgc ttggcttgga cactgtttta gaagaaacct
    22081 cagcaaaacc tggagaggtg actgtagtcg aagttgagac tcctcaatca acaacaaatc
    22141 aggagcaagc taggacagaa aaccaagtag tagagacaga ggaagctcca aaagaagaag
    22201 cacctaaaac agaagaaagt ccaaaggaag aaccaaaatc ggaggtaaaa cctactgacg
    22261 acacccttcc taaagtagaa gaggggaaag aagattcagc agaaccagct ccagttgaag
    22321 aagtaggtgg agaagttgag tcaaaaccag aggaaaaagt agcagttaag ccagaaagtc
    22381 aaccatcaga caaaccagct gaggaatcaa aagttgaaca agcaggtgaa ccagtcgcgc
    22441 caagaaaaga cgaacaggca ccagtcgagc cagaaaatca accagaagct cctgaagaag
    22501 agaaggctgt agaggaaaca ccgaaacaag aagagtcaac tccagatacc aaggctgaag
    22561 aaactgtaga accaaaagag gagactaaaa cagctaaggg gacgcaagaa gaagattacg
    22621 tgggtgaggc tcttgttcca gaacaattac ctgagtataa ggtgactgaa ggaacactcg
    22681 ttgaagaatc aactacagaa cttgactata aaactgaaac gactgaggac ccaaccaagt
    22741 acacggacga agaaactgtc ctacgaaatg gtgaaaaagg aagtcaagtt acaaaaacaa
    22801 cctataaaac aataggaggg gttaaaactg accaagttct ttcaaccagt acggaagtca
    22861 ccaaggagcc tgtaaatcaa caggtaatcc gtggtacaaa accaattgaa gggacaacgg
    22921 ttgagacaag agaagaaatc attccttttg aaacaaaaga acaagaagat gacactttaa
    22981 aacgcggaac aagacaagta actcaagaag gtgtaaacga taagaaacaa attactgaaa
    23041 cctataaaac aattcgcgga gaaaaaacaa gtgacgctcc tactatcaca gaaacagtga
    23101 ttgaaaaacc tcaagacaaa attattaaga atggcacaaa agagttagag aaaccaacct
    23161 tggaatgggc taaaacagat aaagatgtct tgaagaagag tgcgacagct agttatacat
    23221 tgactaaacc agctggagta gaaattaaat cgattaaagt agctctaaaa gataatactg
    23281 gaacagttgt taaagaagta acggtagaag aaaataactt acaagctact ttagacaact
    23341 tgaaatatta tcaaggttat acactatcca caaccatggt ctatgatcga ggtaacggtg
    23401 aagaaactga aatattagaa gataaagaag ttcagttaga ccttaaaaaa gttgagatta
    23461 aagatattaa agaaacaaga ttgatgaaag tggatgagaa tggaaatgaa acagatagta
    23521 gccttctaga aactgttcca gaaaatctaa cgtcatatta tctaaaaatt accactaatc
    23581 ataataaagt aacaaggctt gccattacta atatagagga agtaactgag gaggagacat
    23641 ccttatttaa agttactgct tcagctcctg atttaatcca acgtgattca aataatagtt
    23701 tgagtcaaac atacacttat tacatagaga aacccaaagc atcgcaaaat aacgtttact
    23761 ataacttcaa agatttagta gatgctatgc agaaaaatcc taacggtgag tttaaattag
    23821 gatcggattt aaatgcaaca aatgtaccta caccaagtaa atcatatgta acaggtaaat
    23881 ttacaggtca cttaacaagt gttgatggta agcatttctc cattcataac actgctcgtc
    23941 cattatttga tagagttgaa gggggttcta tcaaaaatat taatttaatt aacgttaata
    24001 ttaatatgcc ttgggcaaat caattagctt cattagctca aacagtgaaa aatgcaactg
    24061 ttgaaaatat taaagtaacg ggtactatcg ttggtaataa caatgttgcg ggtattttgg
    24121 ataaattaga tgatgcaggt attatgacta atgttgcttt tatcggaaaa gttcaatctg
    24181 ttggtgataa agggtggaac ttggctggga ttgcatctga gatttatcga ggcaatttaa
    24241 ataaagctta tgcagatgtt gaacttaaag gtttaaatac tcgtgcaggt gctatagtag
    24301 gacatttagc tcatgatttt aatccaagag atgttggtgc ttggtctaat gtaagaaatt
    24361 ctgtagccaa aggtactatt gatgttgcta gaggtgatgt aggcggttta attggtaaag
    24421 actggccttg gggaaccatt gataacaata tcagcttagt gaatgttaag cgtggtgata
    24481 ttttctttgg ttctaatgat atggatgatg aatttgtaag caattggatt aaacctaata
    24541 atgcaacagt tatcggtgtg agttctggag ataaaaccca taaacaatcg tctaaaatta
    24601 aagaaatttc acaggtagaa gctgataaga aaattaagga tatgggtatc actgctgata
    24661 catttgaagt aactgagcat ttggttgata agttgaatcg ccgtatatta cgtgatgaag
    24721 aatacaaaac aattcaagat tataatgcaa atcgtgagct tgtttatcgt aatgttgaaa
    24781 aactccaacc gttctacaac aaagaatgga ttgtgaatca agggaacaaa cttacaactg
    24841 atagtccatt aatgaataaa gaagtattgt ctgtgactgc aatgaaaggt aatgacttta
    24901 ttacagacct tacagatgct gaccatatta tggttcatta cgcagataag acaaaagata
    24961 tctttacgat ttctcctaaa gactctcaag ttaagcaagt aaaagaatac agtgtcgcag
    25021 agcttggtga agtcgtctat acaccaaata tggttgttaa agatcgtgca gatttaatta
    25081 gtgctattga aggtatttta agtccaattg aattacaatc tgatccaatt tatcaacgat
    25141 taggaagaac tggggataac agagttaatg caattaagaa tctttactta gaagaaagct
    25201 ttaaagaagt aaaagatcat cttaatacat ttgtgacaaa attagttgaa aatgaagatc
    25261 atcaactgaa tacagatgaa gctgcaaaac gtgccttgat taagaaaatt gaagataaca
    25321 aagctgctgt cctgttagga ttaacatacc taaatcgtta ttacggtgtg aaatttgata
    25381 acttcaatat taaagaatta atgctattca aaccagattt ttatggtaag aatgtaagtg
    25441 ttttagatcg attaatagaa attggttcaa aagaggacta tattaaggga agtcgtacac
    25501 aggagtcctt ccgtgaagtg gtggcgaaaa atactctttc tggaaactta catgacttct
    25561 tgacctacaa tatgaaacta ttcacaaatg aaacagacat aaatgtttgg tttaagaaag
    25621 ctattgaaaa taatgcttat gtagttgaac aaccgtcaac taaccctgca tttgctaata
    25681 aaaaatatcg tctatatgaa ggacttaata acggacaaca tggacgcatg attttatcgc
    25741 ttctgaactt gaaagatgca catttattta tgatttcaac atataatact atctcattta
    25801 gttcgtttga aaagtatggt aaagatacag aagaaaaacg tgaaacgttc aagagtgaaa
    25861 tcaataaacg tgctaaagag caagttaatt acttagattt ttggtcacgc cttgccactg
    25921 ataatgtccg cgataaactt cttaagagtc aaaatgtagt tccaactcct gtatgggata
    25981 atcatggtgc acccggtggt tggcctgata gatttggtca tcgaaaagat aaaccggatt
    26041 atgcacctat tcgtgaattc tttggacgta ttgataagta tcatgattat aagtatgggt
    26101 atggagcgta tgcttacatc tttgctgatc cacagcctat ggatgctgta tattttgtta
    26161 tgtcggattt aataagtgaa tatggcacat ctgcctttac acatgagact acccacgtaa
    26221 atgaccgaat ggcatatctg ggaggtcata gacatcgaca gggtacagat cttgaagcat
    26281 ttgctcaagg tatgttacaa actccagcag aacatggaca tcaaggtgaa tatggcgcac
    26341 ttggattaaa tatggcattt gaacgttcaa atgatggaaa ccaatggtat aatccagatc
    26401 ctaccaaact tcaaacacgt gaccaaatcg accattacat gaagaactac aatgaagcta
    26461 tgatgatgct cgattatgca gaagctgagg ctgttcttcc taaggttaaa ggtgacaata
    26521 gtaagtggtt caagaaaatt gatcgcgaaa cacgtcgacc aatggacaga aataaactaa
    26581 gtggtcctca ccaatgggac aaagtgcgtg atttgataga cgctgagaaa acaactaacc
    26641 ttgaaaccat cgatgacttg gtaaataata atttcatgac cattcacgga aatccaggta
    26701 ataaggtctt ccacccagag gactttggta cggcatatgt caatgttaat atgatggctg
    26761 gtatttatgg cggtaatacg agtcaaggtg cccctggttc tctatccttc aaacataacg
    26821 ccttccgtat gtggggttac tatggatatg aacatgggtt cattgactat gtttcaagca
    26881 aacatcaagg tgcagccaat aaagaaaata acggtctatt aggtgatgat ttcattatta
    26941 agaaagtttc taataaccaa ttttcaaacc ttgaagattg gaagaagcaa tggtatgggg
    27001 aagttcttac aaaatctaaa aaaggttttg caccaattga aattaatggc actcaaattt
    27061 caacctatga acagttgaag actatgtttg atgaggctgt tactaaagat ttaacggaac
    27121 taaacgatag tagtattaaa gacaaatacc gaaatacagt tgtcttgaaa tcgaaagtct
    27181 ttaaagctct ccttaaaaac acagacggtt tctctggtga tctatttact gcaccacaag
    27241 catagaatga ataaaatcgt aaaaaggtag atcagctttg tctgtatctg cctttttatg
    27301 ctatacttaa ggtatgcata gaaaaacagt gattgatttt aggactttgg gggagagata
    27361 tacctttacc aagcctatta aagagttgaa aacgagaaat gtagaagaag tggcagattt
    27421 gctggcacaa gtggaaagct accaagagca aggttattat gtggtggggt atgtcagcta
    27481 cgaggctgca cctgcttttg aggaaaaatt agcagttcac aaggatcccc tactgggaga
    27541 gtacctgctt tattttactg ttcatgatag cgtagaaact tcccctattc ctctgactta
    27601 tgaggatatt gatttgccct caaattggca ggaagtaacg tctgcagcag actatgaaaa
    27661 ggccattgcc caaattcacc atcatttacg gcagggagat acctatcagg tcaactacac
    27721 cgtccaactt aagcaaaagt taaatgccaa tccttttgcc atctacaatc gtatggtggt
    27781 agagcaggag gcgggctaca atgcctatgt ggaacatgac gagatggcag tgatttccat
    27841 gagcccagag ctcttttttg agcaaaatga tcgcgagttg acaacacgac caatgaaggg
    27901 gacgactcag cgtggggtaa ctgaccaaga agatcttgaa caggccagtt ggttggaaca
    27961 ggatcccaaa aatcgctctg aaaatatgat gattgtggac ctcttgcgca atgatatgaa
    28021 ccgtatttct gaagttggga gcgagcacgt ggagcgtctg tgtcaggtag agcagtattc
    28081 aactgtttgg cagatgactt cgaccatcaa gagtcagttg cgagaggatg tggaccttgt
    28141 tgaaatcttc cgctcactct ttccttgtgg ttccataacg ggtgcaccga aaattgcgac
    28201 aatggagatt atcaaggact tggagcctca accgcgtgga gtctactgtg gaacgattgg
    28261 tctcttgctt ccaaatggac gacggatttt taatgtggcc attcgtacca ttcaacttca
    28321 caaaggtcaa gccatttatg gagttggcgg agggattact tgggatagca catgggaatc
    28381 tgaataccga gaggttcatc aaaaggcagc agttctttat cgtaaacaag ctcgcttcca
    28441 actgattacg acaggggaaa tcagccagaa gaacctgttg tttgaagatc aacatctgga
    28501 aagactgaga aaagctagtc gttattttgc ctttcctttt gatgcagaag acttgggaca
    28561 caagattgag gaagagtgtc aggattgtga agctaatcga gattaccgct tgcgaatcag
    28621 ccttagcaaa tctggagaga tagaagtcaa tcgtcaagta ttaacccctc tcagtacaag
    28681 cttttgtcag gcccaagtct gccttcagga agctgctttg aatcaatcct ttacctactt
    28741 taaaaccact caccgaccgc atttgagcct aggagaacaa gagaagattt accacaataa
    28801 gtcaggagaa ctgcttgaaa cctctatagg aaatttggtt ctgaaaatcg ctggaaaact
    28861 ctacacaccg cctatccgac ttggaatctt gccaggaatt taccgtcagt atttgctaga
    28921 aacaggacag gtagaagaga aagtcttgac cttggcagat ttagcccaag cagaagctat
    28981 ttacggctgt aatgcagtga gaggcttgta tgaactaagc cttgaggaga actagatgaa
    29041 actgatattt ttgcatgggt tagggcagtc agcagagtct tggaaagaag ttcggaatct
    29101 actgacagat tatccttctg aggctatcga gttatttcct tctggagtta gtaactatca
    29161 acaagctaag gagcgagttt atcagcacct agctcaagag acagaacctt ttgttttgat
    29221 tggattgtcc ttaggtgctg ccctggcact agaactttcc agttacgatt taccaaatct
    29281 tcgagctttg attctgtcag gatgcccgct taaattagca gggaatatcc tattttatct
    29341 tcagttgctg atttttaaac tacttcccaa aagagtattt gaaaaacagg gagcagacaa
    29401 ggctcttatg gtcggagttt ctgaggagtt gaagacactt gatttaacgg atatagcagg
    29461 aacttgccct tatccaacct tactaatttg tggtatcaaa gataaaccga atcttagttc
    29521 tatgagaagt cttcataaac taatatcaga atcccaattt cagattatcc cagatggccc
    29581 tcatgtcttg aataaggcaa aaccaaagga gtttgtagaa aagaccagaa gtttccttga
    29641 attgctgaaa taagtttgat aaaataatat tgaaatcgat gtcataatta tgggagaaag
    29701 aaatgaataa acgtctattt ttaaaaatga gtctggtgac gttgccaatt ttagccttgt
    29761 tttcacaacc tgtgttagca gaggaaaaca ttcatttttc gagttgtaag gaagcttggg
    29821 ctaatggcta ttcggatctt catgagggag aacctggtta ttctgccaag ttggaccgcg
    29881 atcatgatgg cgtggcttgc gaattgaaaa atgctcctaa gggtgctttt aaagcaaaac
    29941 agtcaacggc tattcaaatc aacacaagtt cagcaacaac aagtggttgg gttaagcagg
    30001 acggcgcttg gtactacttt gatggaaatg gaaatctagt gaaaaatgca tggcagggaa
    30061 gctattacct gaaagctgat ggtaaaatgg cacagagtga atggatttat gactcttctt
    30121 atcaagcttg gtattatttg aaatcagatg gttcttatgc aaaaaatgca tggcaaggag
    30181 cttattacct taaatcaaac ggtaaaatgg cacaaggtga gtgggtttat gattcttctt
    30241 accaagcatg gtattacttg aaatcagatg gttcatatgc tcgcaatgca tggcaaggaa
    30301 actactattt gaaatcagat ggtaaaatgg ctaaaggtga atgggtttat gatgccacct
    30361 atcaagcttg gtattatttg acatcagatg gttcttatgc ttacagtaca tggcaaggaa
    30421 attactatct aaaatcggat ggtaaaatgg ctgtcaatga atgggttgat ggtggacgtt
    30481 attatgttgg cgctgacgga gtttggaagg aaggtcaagc aagtacagct tcttctagta
    30541 atgatagcaa tagtgaatat tctgctgctt taggaaaggc aaaaagttat aattcgttat
    30601 tccacatgtc aaaaaaacgt atgtatagac aattaacttc tgattttgat aaattttcaa
    30661 atgatgcagc tcaatatgcc attgatcatt tagatgatta acagatactg gtaggcgaaa
    30721 aaattcctta taattcattt acgcaaacct ttgcataagt gaatggattt tgatatacta
    30781 gtactgtaag cattttcaat tactacacaa tgaaaaagga gaatatgatg agtcaaaaga
    30841 ttattgggat tgaccttggt ggaacttcta tcaaatttgc aatcttaaca acagcaggag
    30901 aaatccaagg aaaatggtca atcaagacca acattttgga tgagggaagt catatcgttg
    30961 atgatatgat tgagtctatt caacatcgtt tggacttgct tggattggca gcagcagact
    31021 tccaaggtat tggaatggga tcaccaggtg ttgttgaccg tgaaaaaggg actgttatcg
    31081 gtgcctacaa cttgaactgg aaaacccttc aaccaatcaa acaaaagatt gaaaaagctt
    31141 tgggcattcc atttttcatc gataatgatg ccaacgtagc agctcttggt gagcgctgga
    31201 tgggtgctgg tgataaccaa ccagatgttg tctttatgac actcggtact ggtgttggtg
    31261 gtggtattgt cgcagaaggc aaattgcttc atggtgttgc tggtgcagca ggtgaacttg
    31321 gtcatatcac tgttgatttt gaccaaccaa ttgcatgtac ttgtggtaaa aaaggctgcc
    31381 ttgagacagt tgcttcagca acaggaattg tcaacttgac tcgtcgctat gccgatgaat
    31441 acgaaggcga tgcagccttg aaacgcttga ttgataacgg agaagaagta actgctaaga
    31501 ctgtctttga tctcgcaaaa gaaggagacg accttgcttt gattgtttac cgtaacttct
    31561 cacgttactt gggaatcgct tgtgctaaca ttggttcaat cctaaaccca tcaacaatcg
    31621 tcatcggtgg tggtgtgtca gctgcgggag aattccttct acaaggtgtt caaaaagttt
    31681 acgatgaaaa tagtttccca caagtacgca catctactaa attagctctt gcaactctag
    31741 gaaatgacgc tggagttatc ggagcagcat cacttgtatt gcaataagat aatgagagag
    31801 gaaaatgcta acctagagtt agtaatgttc ctcttttatt ttttatgcta tactagttag
    31861 gacaatgaat gaggtgagag taaatgacaa aagcagatac gatttttaaa gagaatattg
    31921 aacgcattct aaaggaaggt gttttctcag agcaggctcg tcctaagtac aaggatggga
    31981 ctgttgctaa ttccaagtat gtaactggtg ccttttccga gtacgactta tctaaaggag
    32041 aatttcctat cacaaccttg cgtcccattg caatcaaatc cgccatcaaa gaagttctct
    32101 ggatttacca agaccagtca aatagcttag aagtgcttaa tgacaagtac aatgttcact
    32161 actggaatga ctgggaagtt ggagacacgg gaaccattgg tgagcgctat ggtgccgttg
    32221 ttaagaaaca cgacattatc aataagcttc tcaaacagtt ggaaaccaat ccttggaacc
    32281 gccgcaatat tatttctctc tgggattacc aagctttcga agaaacagat gggctgctcc
    32341 cgtgcgcctt tcagaccatg tttgatgttc ggcgtgttga tggggaaatc tatctggatg
    32401 cgaccttgac ccagcgctcc aatgatatgc tggtggccca ccacatcaac gctatgcagt
    32461 atgtggcttt gcagatgatg attgccaaac attttggctg gaaggttggg aagttcttct
    32521 acttcatcaa caacctccat atctatgata atcaatttga acaagctcag gaattgctcc
    32581 gtcgggagcc gtcaaactgc caaccacgct tggttttaaa tgttcctgat gggactaatt
    32641 tctttgatat caaagcagaa gattttgagt tggtggatta tgaccctgtt aagccacagt
    32701 tgaagtttga cctagctatt taaaagaata gaaaaaagaa gttgagaata atcccaactt
    32761 cttttgtttc ttaacgtgat acgcggcgac gagctgcttt tttacggttt tcttcgatga
    32821 aagctgcttt ttgctcttct ggttcgatta ctttcttttt aaatgcgtat actgcacctg
    32881 caacggcagc gacagttcct gcgacacctg ttacaagacc tttagcgaat cctttagcca
    32941 tgagtcttcc tcctttatat tctcaatcag ccagcctcct caagaggtca catttttctg
    33001 actgaccttt ttgtgttata ataatagtaa cgaaaaaatg ggaatttttc aaggaaaaaa
    33061 gatgaaaaca aaaataattg tgattgttgg accgactgct gttggaaaga cggctctagc
    33121 tattgaagtt gcaaagcgtt ttaatggcga agtggttagt ggagatagcc aacaagtcta
    33181 tcgaggactt gatattggga cggccaaggc tagtccagaa gagcaggcag ctgtttctca
    33241 tcatttaatc gatgttagag agataaccga gtcttactcg gcttttgatt ttgtttcaga
    33301 agctaagatg actattgagg atattcacag ccgtggcaag ctagccatta tcgccggtgg
    33361 gactggactt tatatccaga gcttgctaga aggttaccac ctaggtgggg agactcctca
    33421 tgaggagatt ttagcttatc gagctagttt ggagccatat tcagatgagg aattagccca
    33481 tttggtggat caagcaggcc ttgagattcc ccagtttaat cgtcgtcgtg ctatgcgtgc
    33541 cttagaaatt gcccattttg gtcaggattt ggaaaatcaa gagaccttgt atgaaccgct
    33601 gattatctgc ttggatgatg aacgtagtca actttatgag cgtatcaaca accgagtgga
    33661 tttgatgttt gaggctgggc ttttggatga ggccaagtgg ctgtttgacc attcccctaa
    33721 tgtacaggct gctaaaggaa ttggctataa ggaactcttt ccttatttcc gtggagagca
    33781 gaccttggag gaagctagtg agagtctcaa acaggcgacc cgtcgttttg ccaagcgtca
    33841 gctgacctgg ttccgtaatc gcatgcaggt caccttttat cagattggag aatctggtgt
    33901 acaagaccgc attttaagcc agatagagga gtttttagat gattgaaacg gagaaaaaag
    33961 aggagcgagt cctgctgatt ggtgtggaat tgcagggtat ggacagtttt gacctctcca
    34021 tggaagaatt ggctagttta gcgaaaacgg caggggcagt cgttgtagat agctacagac
    34081 aaaaacgtga aaaatatgat tccaagacct tcgtcggctc tggtaagttg gaagagattg
    34141 cgcttatggt ggatgcagaa gaaatcacta ctgtcatcgt caacaaccgt ctgaccccaa
    34201 ggcagaatgt caatctagag gaagttctcg gtgttaaggt cattgaccgt atgcagttga
    34261 ttttggatat ctttgccatg cgggctcgaa gccatgaagg gaagctccaa gtccacctag
    34321 cccaactcaa atacctcttg cctcgcttgg ttggtcaggg gattatgctc agccgtcagg
    34381 cagggggaat tggttcccgt ggtcctggtg aaagccaact ggaactgaac cgtcgtagcg
    34441 ttcgcaatca aatcacggat atcgagcgcc agctcaaggt ggttgagaaa aatcgggcga
    34501 ctgtcagaga aaaacgtttg gagtctagca cttttaagat tggtttgatt ggttatacca
    34561 atgctgggaa atcaactatc atgaacatct tgaccagtaa gacccagtat gaagcagatg
    34621 agctctttgc gactctggat gcgacaacca agagtattca tctgggaggc aacctccaag
    34681 taactttgac agataccgtt ggctttatcc aagatttgcc gacagagttg gtgtccagtt
    34741 tcaagtcaac cttggaagaa agcaagcatg tggaccttct ggttcatgtt atcgatgcta
    34801 gcaatcctta ccacgaggaa catgaaaaaa cggttctctc catcatgaaa gacctggaca
    34861 tggaagatat tcctcacttg acgctttata ataaagcgga tttggtggag gatttcacgc
    34921 ctacccaaac gccatatacc ctcatttctg ccaagtctga ggacagtcgt gaaaacttgc
    34981 aagcattatt gctagataag attaaggaaa tttttgaagc atttaccctg cgagtgcctt
    35041 tttcaaagtc ctacaagatt catgatttag agagtgttgc aattctggaa gaacgtgatt
    35101 atcaggaaga cggcgaagtg attacaggct acatttccga gaaaaataaa tggaggttag
    35161 aagaatttta tgactgatat taaaactttg gctctaaagt atggaggtta tacaagtctg
    35221 gacaaggtct atctggatca gcttctagct ggtaagacag agcaggagca gttaacactt
    35281 attacacctc cgccaagtgt tgttaatgcc tactttgcag aactctatca gaaaaaaagt
    35341 cctcaggctg cgacggatta ttttgcagaa ctcagtcagg aactgaatct ttacaatact
    35401 gagccaagtt tcaccctaga aaataagtct tttattcggc ttaatctgtc tggtaaatcc
    35461 tttggttttt gttatgagag tgacggcttt ggtcggattt tctctgaaaa tgaggagaaa
    35521 atctcggatg acttgctctt tgaaattgcg caaattttcc cccatcaatt agtctttgaa
    35581 gagtctggta agatttacat gaagcctgtc gaggacgagg aagttgtcag cctagaaagt
    35641 ctcacagctt tgactgattt ggaaagattg gctgatggtc gaaaacgtct caaaggctac
    35701 agccaagagg atttattgca agaagctgct gctttttctg gcaaacgcta tttccgatcg
    35761 gaaaaccgca cagccatgtt atatattgat taattagaaa gtatcgaatg gatattcaat
    35821 ttttaggaac gggggctggt cagccctcta aagctcgcaa cgtttcaagt ctcgccctga
    35881 aacttttgga cgagattaac gaagtttggc tctttgactg tggagaaggt acgcaaaatc
    35941 gcattctgga aaccacaatt cgaccacgta aggtcagcaa aatctttatt acccatctgc
    36001 atggagacca catttttggt ttgccaggtt tcctttctag ccgtgccttt caggccaatg
    36061 aagagcagac agatttggaa atctacggac ctcaaggaat caagtcattt gtcttaacca
    36121 gccttcgtgt gtcaggttct cgtctgccct accgcattca tttccatgag tttgaccaag
    36181 attctctggg taaaattctt gaaaccgata aattcactgt gtatgcagag gagctggatc
    36241 acactatttt ctgtgttggc tatcgtgtca tgcaaaaaga tctagaaggg acgctggatg
    36301 ctgaaaaact caaggctgct ggtgttccgt tcggcccgct ttttggtaaa atcaaaaacg
    36361 gtcaggatct tgttttggaa gacggaactg aaatcaaggc agcagactat atctcagcgc
    36421 cacgtccagg taagattatc actattttag gagacactcg aaaaacggat gccagtgtgc
    36481 gtctggctgt caatgcagat gtcctagttc atgagtccac ttatggcaag ggtgatgaaa
    36541 aaattgctcg taaccatggt cactcaacta atattcaagc tgcacaagta gcggtagaag
    36601 caggtgccaa acgcctccta ctcaaccata tcagtgcccg tttcctctca aaagatatta
    36661 gcaaactcaa gaaagacgct gccacaattt ttgaaaatgt ccatgtggtc aaagacttgg
    36721 aagaagtgga aatctagcag tcacagaaag gataagtatg cctactattc tcattaccgg
    36781 agctagcggt ggtctagctc aagaaatggt caaactcctg cccaatgacc aactcatctt
    36841 gcttggtaga aataaggaaa aattagccca actctacgga aattattccc atgcagaatt
    36901 gattgaaatt gatattaccg acgattcagc cctagaagct ctggtaactg atctttatct
    36961 ccgctatggc aagattgatg tcttgattaa caacgctggt tacgggattt ttgagggatt
    37021 tgaccagatt gctgataaag atattcacca gatgtttgag gtcaatacct ttgccctgat
    37081 gaatctgtct cgtcaccttg cggctcgtat gaaggaaagc agcaaagggc atatcatcaa
    37141 catcgtcagc atggcaggtc taatagctac tggcaagtct agtctttact cagcgaccaa
    37201 gtttgcggct attggttttt caaatgctct gcgactcgaa cttatgccct atggagtcta
    37261 tgtgacaaca gtcaatccag gtccaatccg aacaggattt tttgaccaag ctgacccaga
    37321 tggaacttat cttaaatcgg ttgaccgctt cctcttagag gcagatgcag tggctaaaaa
    37381 gattgtcaag attataggca aaaataaacg agaactcaat ctcccgattt tgttgaacct
    37441 agcccataag ttttatactc tctttcccaa gctagctgat aagttggcag gggaaacttt
    37501 taattataag taaaaagaac caatgtgcag gttgttgcta gcctacatat tggttcttat
    37561 catttctcaa ctatcaaact gaacttcttc taggagatag tctataaagc gttcgcccat
    37621 cttagatagg ctggttttct catgctggat atagaccagc tcgatcgggt catcaatatc
    37681 cagtgggata gaaacgatat tgtctccgtt taggttgctg ttcaaaatcc ctgtcgcaat
    37741 ggtataacca tccaaaccaa tcaagagatt aaagagggtg gcacggtcac tgaccacaat
    37801 ggatttcttg tggtgttctt gagaaagaat ctcttctgaa aagtagaagg agttgtgcgt
    37861 cccttggtca tagctgaggt aagggaaatt ctccaaatca gacagtttca ccttgtcttt
    37921 ctttgccaga gggttggtct tgctgacaaa gatatgcggt tgcgctgtga agagatggtg
    37981 ggatagcagg tgattgtcat ccagcatctt ggttaaaaca tcacggttgt aactgtttaa
    38041 gaagaggacc ccgacctcac tgcggaagtt cttgacgtcg tcgataatct cccaagtccg
    38101 agtttcacga aggaagagtt cgtatttctc catatcgctt ttcttgagca aagagacaaa
    38161 ggcattgacc acaaaggcat agtgttgaga cgaaacgcta aagagttcgc ggtgggcgac
    38221 aggattttta tagcgttcct ccagaagctg ggtctgctcg acaacctgac gggcataaga
    38281 gagaaactcc atcccatcac gggtcaaggt gattcccttg ggattgcgga taaagatctc
    38341 aatgcccatt tcattttcca aatctcgcac tgcattggag agacttggct gagtgataaa
    38401 gagttgcttg gctgcctcat tcatggagcc agtttcgacg attttgataa tataatgtaa
    38461 ttgttgaatt ctcatgtttt tattgtacca tacttgcctt agaatcgcta aagaagatag
    38521 gaaaataagg agcattgtgt gagtctcttc ttgaaaaaaa cacaaaattt tgctagaatg
    38581 agtcttatga aaacattcta tgatgtgcag caattcctca aacgatttgg tattattgtt
    38641 tacatgggaa aacgcttata tgatattgaa ctgatgaagt tggaactctc tcggatttac
    38701 gatgcagggt tgatggataa attagactat ctagaggcag aagcggttct tcgcagagag
    38761 cacaaggtag aattggatta tattgagaaa aatggagaaa agaactaatg gttacttgga
    38821 ttttgtgggc acttatacta gcaatgttgg cgtggatggg ctttaactat cttcgtattc
    38881 gccgtgcggc taaaattgtg gacaatgagg agtttgaagc cttgattcgt acgggtcaat
    38941 tgattgattt gcgcgaccca gcagaattcc acagaaaaca tatccttggt gcacgcaata
    39001 ttccttcaag tcagttgaaa actagtcttg cagcccttcg taaagataaa cctgtccttc
    39061 tctacgaaaa ccaacgtgcg caacgagtta caaatgcagc tctttacttg aaaaaacaag
    39121 gtttttctga gatttatatc ctttcttatg gcttggattc ttggaaaggg aaagtgaaga
    39181 ctagctaacc ttatttccaa attagtcgaa tgataggatg aagctattgt tttatagtat
    39241 aattaatttg tataaaattt aaagagttct tagaacatga aagaaaagaa gaaaaatcca
    39301 ctttttgtgg gtatcttgtc gattattctt ggtttgttat ttccaattgt aggcctgatt
    39361 ttgggtatca taggattggt cttggctatt tcataccaaa aagagtcaca attagactat
    39421 aaaatagaaa agattcttaa tatcttagga attgtgattt ctgtagttaa ctggattgta
    39481 gcaatcgcct taatttttcg gtaaataaaa gaaaaaagct ttagttttaa agcttttttt
    39541 gttttatcga ctcatctcta aatagttttt aatgatttgt aactcttttt ggttcaaagg
    39601 gcggtagtta ccttctgcta aatctgggtt caatgtgaag tccccaaatt ggattctctt
    39661 gaggatagtc accttaacac caaccgagag gaacattttc ttgatttgat gaaattttcc
    39721 ttctgaaatg gtgatagagg cttgactgag ggaaggactt gcagatagaa tctctagtct
    39781 tgcaggttta cagatagtgc catctaaaaa gacaattcct ttttgaaagg tttggatatg
    39841 gtcaggtgtt agaagtccat taacctcaac ttgataagtc ttatcgacat gatattgggg
    39901 atggaggagt tggaagccca agggaccgtt atcggtcaag agaaggagtc ctgttgtatc
    39961 tcgatccagt cggccgacgg catagagctt gtcagactgg atgtcagatg gaagcaagtc
    40021 catgacggtc ggaagttcct tgtctttgtt ggctgtaacg acaccagcag gtttatgaag
    40081 cataagatag gtgtgttcat agccttgaat gattcgatcc tgaaaaagga gttcttgtag
    40141 tcctgtatcg atattttgag ctagggagcg ggctgggcaa ccatcgacta gaatgtcccc
    40201 tttgagtaag gcttgtttca tggcctttcg gctgattttt tcttgagcta ataaattatc
    40261 taaacgcata ctgtgcctat cttatcataa gtcgcttgct ttgaaaaggc ttgacgtgaa
    40321 aagaaaagcg gagtgaaaac atttacactt gcttgagtta tgttatttat ttgaaattat
    40381 ggtataatcg ttcagttaga aaataaattt tgaatattat agaggaaatc atgacaaaat
    40441 taagagaaga tatccgtaac attgcgatta tcgcccacgt tgaccacggt aaaacaaccc
    40501 tggttgacga attattgaaa caatcagaaa cgcttgatgc acgtactgaa ttggcagagc
    40561 gtgctatgga ctcaaacgat atcgaaaaag agcgtggaat caccatcctt gctaaaaata
    40621 ctgccgttgc ttacaacgga actcgtatca acattatgga cacaccagga cacgcggact
    40681 tcggtggaga agttgagcgt atcatgaaaa tggttgacgg tgttgtcttg gtcgtagatg
    40741 cctatgaagg aaccatgcca caaactcgtt tcgtattgaa aaaagccttg gaacaagacc
    40801 ttgtcccaat cgtggttgtt aacaaaatcg ataagccatc agctcgtcca gcagaagtag
    40861 tggatgaagt cttggaactt ttcatcgagc ttggtgcaga tgacgatcag cttgatttcc
    40921 cagtggttta tgcttcagcg atcaacggaa cttcttcatt gtcagatgat ccagctgacc
    40981 aagaaacgac tatggcacca atctttgaca cgattatcga ccatatccca gctccagtag
    41041 ataactcaga tgagcctttg cagttccaag tgtcactttt ggactacaat gacttcgttg
    41101 gacgtatcgg tatcggtcgt gtcttccgtg gtacagttaa ggttggggac caagttaccc
    41161 tttctaaact tgacggtaca actaaaaact tccgtgttac aaaactcttc ggtttctttg
    41221 gtttggaacg tcgtgaaatc caagaagcca aagcgggtga cttgattgcc gtttcaggta
    41281 tggaagacat ctttgtcggt gaaaccatca ctccgacaga tgcagtagaa gctcttccaa
    41341 tcctacacat cgatgagcca actcttcaaa tgactttctt ggtcaacaac tcaccatttg
    41401 ctggtaaaga aggtaaatgg gtaacttctc gtaaggtgga agaacgcttg caggcagaat
    41461 tgcaaacaga cgtttccctt cgtgttgacc caactgattc accagataaa tggactgttt
    41521 caggacgtgg agaattgcac ttgtcaatcc ttatcgaaac aatgcgtcgt gagggctatg
    41581 aacttcaagt atctcgtcca gaagttatcg taaaagaaat cgacggtgtt aaatgtgaac
    41641 catttgaacg tgtacaaatc gacactccag aagaatacca agggtctgtt atccaaagcc
    41701 tttctgaacg taaaggtgaa atgttggata tgatttcaac tggtaatggt caaactcgtt
    41761 tggtcttcct tgttccagcg cgtggtttga ttggatactc aactgagttc ttatcaatga
    41821 ctcgtggtta cggtatcatg aaccatacct tcgaccaata cttgccattg attccagggg
    41881 aaattggtgg acgtcaccgt ggtgcccttg tttctatcga tgctggtaag gctacaactt
    41941 actcaatcat gtctatcgaa gaacgtggta cgatctttgt caacccaggt actgaggttt
    42001 atgaaggaat gatcatcggt gaaaactctc gtgaaaatga cttgacagtt aacatcacga
    42061 aggcaaaaca aatgaccaac gttcgttcag ctactaagga ccaaacagct gttatcaaga
    42121 cgcctcgtat cttgacactt gaagagtctc ttgagttctt gaacgacgat gagtacatgg
    42181 aagtaacgcc tgagtctatc cgtttgcgta aacaaatcct taacaaagca gagcgtgaga
    42241 aagctaacaa gaagaaaaaa tcagctgaat aagagctaga aagagagaaa gatggtctat
    42301 ttagtcctag gaattttact gctcctactc tatgtatttg cgacaccaga aagcattaaa
    42361 gggacagtca atatcgtcgc tatggtatgt attttagtgg cactcttgat tttattggtt
    42421 ctatcttttc tgaaaatttt tcaattacca acagaaatat tcctagcaat agccatgttg
    42481 atcctagctt actttagtgt tagagacatc acactcatgc cagtcaaaaa aagtaaaaga
    42541 agataaaaaa gagagtttcg gctctctttt tgctaggaat tttataatag tttgaattgc
    42601 tttttagtta acaccatctc ttttgcttgt atagtatgaa tgggaaaaac ttgaataaaa
    42661 ttcgttaatg gcatctattt tatatttggt tagtactggt tgcatattat tttgaaataa
    42721 aactactata taatattatt catagagata atatagtaaa acatctaaaa aattcgattt
    42781 tttccttgac aacaaataat aaaaatgtta ttcttatcac ataagaaagc ggtttcaata
    42841 aaagagagag gaggatttat tttgttgcat tgttgctcaa ggtgtgattt ttttgaaaga
    42901 aaaaataaaa tgaaaacgaa aaaacataga ttacttgctc tagctcttat ttcaagtttt
    42961 acattattgg gagctgcatc agctgctgta taatatccag atggaggagt atggacatat
    43021 ggagaaggtt caggaggtgg ttaggctttt tcaaattact atcatggtaa aaaatatcat
    43081 tattcatcca tcgtgagcag atgggatggt cattctgata aaggagaagc tcctgctgga
    43141 aaaacctcct atgcatggat ttggactaaa tggggagaac aagtagcatt ttactgtgat
    43201 tatgactaat cgttcattga tttaaatcgt tccccttgta agaaattaca gggggaattt
    43261 tgtttacaag gagtctacta tgaagaaact attcatatta ttatcaactt tttttctcag
    43321 cttcttcctt gcttggatta ttgtcttacg tgcgccacaa tatttatatg caagctatga
    43381 ttccgtttct ttacttcgtg tcaaaaaaga tactcaggaa ccgacgcgtg aggtatttga
    43441 acaggaattg gagaattttg caaactcaga acagagttta atagctagaa gaattgtaga
    43501 gccgagtaag gatgggacga ctcactttac ttatgcaact tatggtcagg gaactttacc
    43561 aaaagaattc caagaagcta gccaagaaag tcgtgaacgt agtgatccgc taaatagtta
    43621 tctccttttg tcaggctcct tgacgaaaga aaagcttgcc gataaattag gagatttggg
    43681 ttataaagca agtgctgacc gaaagatacc gccctatttt cttgcttttc gaatattact
    43741 aaatcccctt attttaatta gtttagcaat atttggctta tctttctttg ctttagtgat
    43801 tatcactcgg attaaggaaa tgagagcagc aggtataaaa ctcttttctg gtcagactct
    43861 cttatccatc atgggggatt ctttatctac tgatatcaag tggctccttc tatcagccct
    43921 cctttccttc ctaggtgggg gagtcgttct ttttagtcaa ggtttgtttt atcctatctt
    43981 gttagccacc tatggttttg ggattagttt ctatctgttg tttttattgg cgatttcaat
    44041 tttactaatg cttctttatc taatgagttt gagttacaaa gcattagttc ccgttattaa
    44101 ggggagatta ccccttaaac gcctgatgat tttaacccta ttgtgtcagt tggttgctgt
    44161 ctttacagta ggatacgctg ttaagacagg tttgacgtct taccaacgat tgaaagaact
    44221 tgaaatttca aaacaagcat ggcaggatag agcagactat tatcaaattt cttttggctt
    44281 aggtgataga ggaaaagata cagaaaatca gagcaagtga tatgcctttg ccaaggaagc
    44341 aatcgaagaa gaacaagctc tttatgtaaa ggataatctg ctccattttg ccaatccaca
    44401 aggaaaaaat gaacagggag agacactgga tacctatagt ccagatgcta atacgctcta
    44461 tgttagtccc agttatttgg acaaggaaaa ggtcgtggta gatgctgaga ccaaacagaa
    44521 gttagcccat cttcaaaaag gtgagtttat cctcttgctc ccagaacatt tgcgctctcg
    44581 agaagcagaa cttaagaaag tttttgaaga aagattgagt tattatggaa aatctggtga
    44641 ggaggcaagt gctcctttgg attatgagat gaaagcgcac gttagttatc tttcaatggg
    44701 agaaaagcgg tttgtttata ataacggtga gaatcccgta tccactcagt atttgactga
    44761 tccgatttta gtggtattca cgccgacttc tacaggtgat agttttatat ccttatctag
    44821 ttggtctatc aatgctggaa aacaactctt tatcaaagga tatgagagtg ggctagaact
    44881 cttgaagaaa gctggaattt atgagcaagt atcctatctt aaagaaggaa gaagtgttta
    44941 tctaactcgt tataatgaag ttcaaactga aacagcaact ttaatcttag gagctattgt
    45001 tggaatagcc agttccttgt tactctttta ttctgtcaat cttctatatt tcgagcaatt
    45061 ccgccgagat atcttgatta aacgaatttc aggtttacga ttttttgaaa cacatgctca
    45121 gtatatggtt agccaatttg ccagttttgt atttggtgct agtctcttta ttttaagcag
    45181 tcgagacttg gtgattggct tgctcacttt attggtcttt ctagctagtg cagttttgac
    45241 gctttaccgt caagcgcaga aagaatctcg tgtttctatg acaattatga aaggaaaata
    45301 ggatgattga actaaagaat atatctaaaa aatttggaag ccgtcagcta ttttcagata
    45361 tgaatcttca ttttgaaggt gggaaaattt atgccttaat cggtacaagt ggctgtggta
    45421 agacaacact cttgaatatg attggacgat tagagccata tgacaaaggg caaatcatat
    45481 atgatggcac ttctcttaag gacattaagc cttctgtttt ctttagagat tacttaggat
    45541 acttatttca agattttggc ttaattgaaa gccaaaccgt caaagagaat ctcaatctgg
    45601 gtttagttgg taaaaagttg aaggaaaaag agaaaatctc tttgatgaaa caagctctaa
    45661 accgtgtaaa cctctcttat ttggatttga agcaacctat atttgagtta tcaggaggag
    45721 aagcacaacg tgttgcacta gcgaagataa ttttaaagga tccacctttg atcctcgcag
    45781 atgaaccaac cgcttcctta gaccccaaaa actctgagga attactttcc atcctagaat
    45841 ctttaaaaaa tccgaatcgg accattatta ttgcgaccca taatcctctg atttgggagc
    45901 aagtggatca ggtcattcga gttaccgatt tatcacatag atgatatggt aagattcagt
    45961 tagaagaaag agtcacaaac acactttgtg gcttttttat ttccataaaa atggtaaaat
    46021 agtaggagta gaaatggagt tcgagacatg aaagtaatag atcaatttaa aaataagaaa
    46081 gtccttgttt taggtttggc caagtctggt gaatctgcag ctcgtttgtt ggacaagcta
    46141 ggtgccattg tgacagtaaa tgatgggaaa cctttcgagg acaatccagc tgcccaaagt
    46201 ttgctggaag aagggatcaa ggtcattaca ggtggccatc ctttggaact cttggatgaa
    46261 gagtttgccc ttatggtgaa aaatccaggt atcccctaca acaatcccat gattgaaaag
    46321 gctttggcca agggaattcc agtcttgact gaggtggaat tggcttattt gatttcagaa
    46381 gcaccgatta ttggtatcac aggatcgaac ggtaagacaa ccacaacgac tatgattggg
    46441 gaagttttga ctgctgctgg ggaacatggt cttttatcag ggaatatcgg ctatccagct
    46501 agtcaggttg ctcaaatagc atcagataag gacacgcttg ttatggaact ttcttctttc
    46561 caactcatgg gtgttcaaga attccatcca gagattgcgg ttattaccaa cctcatgcca
    46621 actcatatcg actaccatgg gtcattttcg gaatatgtag cagccaagtg gaatatccag
    46681 aacaagatga cagcagctga tttccttgtc ttgaacttta atcaagactt ggcaaaagac
    46741 ttggcttcca agacagaagc cactgttgta ccattttcaa cacttgaaaa ggttgatgga
    46801 gcttatctgg aagatggtca actctacttc cgtggtgaag tagtcatggc agcgaatgaa
    46861 atcggtgttc caggtagcca caatgtggaa aatgcccttg cgactattgc tgtagccaag
    46921 cttcgtggtg tggacaatca aaccatcaag gaaactcttt cagccttcgg tggtgtcaaa
    46981 caccgtctcc agtttgtgga tgacatcaag ggtgttaaat tctataacga cagtaaatca
    47041 actaatatct tggctactca aaaagccttg tcaggatttg acaacagcaa ggtcgtcttg
    47101 attgcaggtg gtttggaccg tggcaatgag tttgacgaat tggtgccaga cattactgga
    47161 ctcaagaaga tggtcatcct gggtcaatct gcagaacgtg tcaaacgggc agcagacaag
    47221 gctggtgtcg cttatgtgga ggcgacagat attgcagatg cgacccgcaa ggcctatgag
    47281 cttgcgactc aaggagatgt ggttcttctt agtcctgcca atgccagctg ggatatgtat
    47341 gctaactttg aagtacgtgg cgacctcttt atcgacacag tagcggagtt aaaagaataa
    47401 aatatgaaaa aaattgtctt tacaggtggg gggacggttg gacacgttac cctcaatctt
    47461 ttgttaatgc ccaagttcat cgaagatggt tgggaagtcc actatatcgg ggacaagcgt
    47521 ggtatcgaac accaagaaat ccttaagtca ggtttggatg tcactttcca ctccattgcg
    47581 actgggaaat tgcgtcgcta tttctcttgg caaaatatgc tggacgtctt caaagttggc
    47641 tggggaatcg tccaatcgct ctttatcatg ttgcgacttc gtccacagac ccttttttca
    47701 aagggaggct ttgtctcagt accgcctgtt atcgctgcgc gtgtgtcagg agtgcctgtc
    47761 tttattcacg aatctgacct gtctatgggc ttggccaata aaatcgccta taaatttgcg
    47821 actaagatgt attcaacctt tgaacaagct tcgagtttgg ctaaggtcga gcatgtggga
    47881 gcggtgacca aggtttcgga caaaaacact ccagaaccag atgaattggt ggatattcaa
    47941 acccacttta atcccaaatt gccaactgta ttgtttgtcg gcggttctgc aggcgctcgt
    48001 gtctttaacc aattggtgac agaccataag aaagaactaa cagagcgcta caatattatc
    48061 aatttaactg gagattctag cctgaatgag ttgaggcaaa atctttttcg tgttgactat
    48121 gtgaccgatc tctatcaacc cttgatggaa ttggctgata ttgttgtgac acgaggtggt
    48181 gccaatacga tttttgagct cttggcgata gcaaaattgc atgtcattgt gccgcttggt
    48241 cgtgaagcta gtcgtggtga ccagattgaa aatgcagctt actttgtaaa aaaaggctat
    48301 gcagaagacc ttcaagaaag cgatttgacc ttggatagtt tggaagagaa gctttctcac
    48361 ttactaagtc acaaggaaga ttaccaagct aagatgaaag cttctaagga attgaaatct
    48421 ctagcagatt tttatcaatt gttgaaaaaa gatttatcat aaggaaagta aatgtcaaaa
    48481 gataagaaaa atgaggacaa agaaatcctc gaagaattga aagagttatc agaatggcag
    48541 aaacgaaacc aagaatatct aaaaaagaag gctgaagaag aggcggctct agctgaggag
    48601 aaggaaaagg aaagacaagc tcgaatggga gaagaatctg agaagtcaga ggataaacag
    48661 gaccaggaga gtgaaacaga ccaggaagat tcagaatcag ctaaggaaga gtctgaagaa
    48721 aaagtggcat cctcagaggc tgacaaagag aaagaagaaa aagaagagat agaagaatca
    48781 gagtctaaag agaaggagga acaggataaa aagcttgcta aaaaggctat aaaggaaaaa
    48841 ccagccaaag caaagattcc tggtatccat atcttgcgag ccttcacgat tttatttcca
    48901 agtctgcttt tattgattgt ctctgcctac ttgctcagtc cttatgcgac catgaaagat
    48961 attcgtgttg agggaacggt gcaaactaca gctgatgata ttcgacaggc ttcaggcatt
    49021 caggattcgg attatacgat taaccttctg ctagacaagg caaaatatga aaagcagatt
    49081 aagtctaact attgggttga atcagctcaa cttgtctatc aatttccaac taagttcact
    49141 attaaggtca aggaatatga tattgtggcc tactatattt ctggtgaaaa tcattatcct
    49201 attctttcca gtggtcagct tgagactagt tctgtgagcc tgaacagttt accagaaact
    49261 tatttatcag ttctctttaa tgatagtgaa caaatcaagg cttttgtctc agaacttgct
    49321 caaattagcc cagaactcaa ggcggctatc caaaaggtgg aattagcccc aagcaaggtg
    49381 acatccgatt taattcgatt gaccatgaat gattcggacg aagtcttggt tcctctatct
    49441 gaaatgagta agaaactgcc atattacagt aagattaagc ctcaattgtc agaaccgagt
    49501 gtggtcgaca tggaagctgg aatctacagc tacactgtgg cggataaatt aattatggag
    49561 gctgaggaaa aagccaaaca agaggccaag gaagctgaga aaaaacagga agaagaacag
    49621 aaacgtttag aagaagaaca gaaaaaacaa gaggaagaga gcaatcgaaa ccaaacgacc
    49681 cagcgctcat cgcgtcgcta ggtttacctt ttctcttata gttctttagt gaccatgttt
    49741 ttacttgaca agtgctagta gaaataatag aatagtaaaa aacctttaaa gcagtccaga
    49801 gaggcagcta aggttagacg gtgaaagggt ggagactacc catttttcgt ggaaccttgc
    49861 tgttggcagg ttcctttttt cgtggcttct gttggccaga ctctctcact agtaaaggta
    49921 aaaggagaaa cctatgcgag aacatcgtcc aatcattgct cttgattttc ctagttttga
    49981 ggcggtcaag gaatttttag ctcttttccc agcagaagaa agcctttatc tcaaggtagg
    50041 gatggagctt tattacgcag cggggcctga gattgtgtcc tacttaaaag gtttgggtca
    50101 tagtgtcttt ttggatctca aacttcatga cattcctaat acagtcaagt cagccatgaa
    50161 ggtcttgtct cagcttggtg tcgatatgac taatgtccat gcggctggtg gtgtagagat
    50221 gatgaaggcg gcgcgtgaag gtcttgggag tcaagccaaa ttgatcgctg taacgcagct
    50281 gacatcaacg tcagaagccc agatgcagga gtttcaaaat atccaaacca gtctgcaaga
    50341 gtctgtgatt cactatgcca agaagacagc tgaagctggc ttggatggtg ttgtttgctc
    50401 ggctcaggaa gtacaagtca tcaagcaggc taccaatcca gattttatct gtctgacacc
    50461 agggattcgt ccagctggtg ttgcagttgg agatcaaaaa cgagtcatga cacctgctga
    50521 tgcctatcaa atcggcagtg actatatcgt agtgggacgt cccattaccc aagctgagga
    50581 tcctgttgca gcttatcatg ccatcaagga tgaatggaca caggactgga attaaagaac
    50641 tagattagaa aaataaaagg agaataccat gacacttgct aaagatatcg ctagccacct
    50701 cttgaaaatc caagccgttt acctcaaacc agaggaaccc ttcacttggg catctggtat
    50761 caagtcaccg atttacactg ataatcgtgt gacactagcc tatccagaaa ctcgtaccct
    50821 aattgaaaat ggttttgtgg aagctatcaa agaagccttt cctgaagtag aagtgattgc
    50881 aggaactgca acagcaggga ttccacacgg agccattatt gctgataaga tggacttgcc
    50941 ttttgcctac atccgtagca aaccaaaaga ccacggagct ggtaatcaaa tcgaaggtcg
    51001 cgtagctcaa ggtcaaaaaa tggtagtggt tgaagacctt atttcaacgg gtggttcagt
    51061 tcttgaagct gtagcagcag ccaagcgaga aggagcagat gtacttggag ttgtagcgat
    51121 tttcagctac caattgccaa aagcagataa gaactttgca gatgctggtg ttaaacttgt
    51181 gacgctttca aactatagcg agcttatcca tctagcccaa gaagaaggtt acatcacgcc
    51241 agagggcctt gatcttctaa aacgctttaa agaagaccaa gaaaattggc aagaaggtta
    51301 ggtcagtaag ataaagagag acgaggctac cgagtctctt ttaccatttt atttaaaata
    51361 tgacaatatt ttaaataaaa taattgacaa cgctttcttt tattgatata atgaaaatgt
    51421 aatatgtatc aaattgatca atcaactatc ttgtttatca cttcttaaga ggacgatgtc
    51481 atgaaaagaa gaaaaatata ttcctttttt gcgtagttac aattgcatct agtttgtttc
    51541 tccctacagt tttagctaga cagattggaa attatgattt aacgttgccg cgttggggtt
    51601 cggatacaac tagtgagctt gagaaagaaa acgcctctgc tggaattaat aatagtgaca
    51661 gcactggtgg cggtaaaagg ttaagtacct ctattcgtag cgcctatagt gggtcagata
    51721 ttaccccggt atattcattg gggtctggct ctaggattgt catgtactat aatggaggtg
    51781 gtgacaatta tattggttct ggtactagat tagctatggc gccacaattt ggaaatcatg
    51841 taagaattca tacttcaggt tcttggagtc cagattctta ttaacttact tgtcagagta
    51901 agccttaaag atggttgatt gtgggtgtag catgaaaaaa gaatgctaca ccctattttt
    51961 attataagga ggagtaagga tggaattttt catttgtaat cttgtacgag tcgttcaatc
    52021 acctcgattt tatatgtctt tatttttgac ccttctttgc atgagtttag gaaatttcct
    52081 tgctttcaat ggtatttata aaattgaagg tttatcgatt ttttttgccg cttcttctat
    52141 tcgaggattt tcaccgatta gcctagtagc tgcacttatc tgtacactgc cctattctag
    52201 tcagataata gaggatgctg agagtcattt tctaacagca caattgtgcc gaatttctaa
    52261 aaagaggtat ctagctattg taggtagtac tgcaattatt tcttcttttc tagtcttttt
    52321 tctcccctat ttattattat taggaattaa tcttttagtg actccttatc aggaaattta
    52381 tattggagat tatagtggtg ccttaaaaga attatttgat tctaatcagt tgctctatag
    52441 tcttgtgacg actctctggt atggagtttg gggcgctgtg ttctctattt ttggactagc
    52501 tagtgctttg ctagtgaaga aaaaaatagg agctattttc atcccagttg cctatatgat
    52561 ggttggtggt atttttttgg ctattttagg gctatcttac ttagaacctg tgacaacatt
    52621 agctttggga tatcagaaag atatcagtct ttccttagtt agtgggcatc ttgcttttat
    52681 tttatttgtt agttgtttgg ttgtttatgg tacatttttt ctacattcag aggactatgt
    52741 ataatgaaac aatttgttca attttataaa aaagatttct tagcagtatt ggtttatttt
    52801 atattactgc tatcctgtgt tttatctagt acagtatatt tattgcgctg tcgccaatat
    52861 tcaatccatc caaatgtatt agaatggatc ttagttttac ttcaagatat gacgactgga
    52921 gtatattgct ttccgttcac atatatattg ttcttttttt atttgatgaa taactatttt
    52981 aataggttgg agtgtcgcat tcgtctgaaa tcaattaagc actttaccag ttttagtttc
    53041 aaattagcag ctcttagtac ggggatttgg acggcgattt tatttttatt gatttttcta
    53101 attgcattta gtaatggttt tagcttctct ttggagataa aggaggttga ttttttaaga
    53161 gaattttatg gcataagtat tgcaaacaat gctagtttct ttataggatt ttttttctct
    53221 tatatagcat actatttctt tttatcctta cttactatta gcagtttttc ttggtttaaa
    53281 aaatcaaaca tgagcttagt atttctgttt acttttttat ttgtagaatc cttattctgg
    53341 atttatcagt tgaacaatgg gataattgga ttattgccaa tttttcagta tatggtaaat
    53401 tccaatccgt atgcattgat ttattggctt gcattactat ctatcataat tccattgact
    53461 gtattttctg ttcatagaaa ctggaggaga gtgtaaaagt tggaaatggg aaagttaagt
    53521 agtcacatgt ggaggttgaa tcagataatc tataccaagt acttttgggg ttatgttctt
    53581 ttttggatat tgatttgttt aggattatgg tattggttag aaggaaatga tagacttgtt
    53641 atagaaattt taaaagggcc taatctgagt caaaactctt ttttagtctt atctatatgg
    53701 ttgcttcatt ggtttattat tcatacattt tttctagcag ttgtatatcg tagaagagca
    53761 tccgatttct ttctggaagt gattcgattt tcttctatta agctctggat taggtatcag
    53821 atttggacct gttttcttta tggacttatt ttaatcatgg taaaagttct agtgattcaa
    53881 tttatgttac agttaccaaa ctgggatata ggagttttgt ttatagttga ttctttgaat
    53941 gcttgtgtgc tatccttgct ttgctttatg ttatacgcac taggagcgaa tgtacaaatg
    54001 aactttgctt gcgttagttt ctttttactc atgattgtgt ttggtggtct atttgtagga
    54061 aatcgaacta attatctatt ttatattttg aatcgaggga atggagatat aggacgagat
    54121 ttatttcttc agttgctatt tttagtcttt ctttttaaaa gcattttcta tttcactcgt
    54181 caaaagagga ggtttataga atgaatgaaa taattacatt aaaaaatatt gagttgaaat
    54241 taaaaaaaac atgtgttttt caaaacctta attttagttg taaacagggg gaaattatag
    54301 gaattactgg tgcgaatggc tcaggtaaaa gtgtattgtt taaattaata gctggtttat
    54361 atagtccgtc ttatggagaa gtgttaatca atggggaaaa tattgttcct gagagaaaaa
    54421 ttccagctaa tttgggagct ttgattgaag aacctggttt tataaattat tatagtggct
    54481 ttaagaattt acaatatttg gcaagcatac gaggagtagt tggtaatcag gaaatcaatg
    54541 atacactgaa aatagttggt ctatatgagc aaaaagacca gaaagttaaa acttattcgc
    54601 taggtatgag gaaaaagcta gggattgctc aagcaattat ggagaatccc tctattcttt
    54661 tactagatga acctatgaat gccttggata aatcaagtgt agaaaatatg agaacattgt
    54721 ttagaaagct ctctagtgaa aaagggacaa caattttgat tgctagtcat agtgaagagg
    54781 atattcgtat cttatgtgat aaagtatatg caatagaaga taaagtatgt acactgtgtt
    54841 cagattgaac atcttagata gactcaggat gttataggta aaatcttaaa aagggattgg
    54901 agcttaaaat ccagttcctt ttaagagtta atgactaatc aacttttcca ccttcaacaa
    54961 ccaggtcatc tgctttagca gtgtcaccgt aggttgggtg aagtgttttt tcataggcct
    55021 tgtggaagaa gttttccttg cctaattttt caatatcttt attgatgaag tctagcaatt
    55081 cttggttgcc tttttgaact gctgccgcaa tggtatcggg atcaccgagg gaagtaattc
    55141 ctacttcaaa tcctttattt tcaagcgccc aagctagaac ttccgtatta tcagttgaaa
    55201 aggcatctcc acgtccgtca agaagagctt ggtaagagtc actgtattgg tcgtattttt
    55261 ggagtttgat ttctggatga ttcttttcaa aataagtctc agcagtcgtt ccttttgtga
    55321 caattaaggt tttaccttca agttgtttga cgtctgtaat gagaccagtc ttaggtgata
    55381 cgacacccag agaaactttc atatatggaa gggcaaaatc aacttgtttc ttacgttcgt
    55441 cagttactgt aaagttagca agaataatat ctaccttgtt tgaaatcaag tattccgcac
    55501 ggttggcagc atcgactgaa atgtatttaa ccttgacacc aaggttttga gctagttggt
    55561 tccctagttc aatatcgtag ccttggtaag aaccatcatt gtcaacgtag ccaaacagtt
    55621 ttttatctcc aaacacggcg attcgcagtt caccgctttt tttgatttca tcgatagtgc
    55681 gagctttggc agttgttttt ccagaggatg aaccagcgtt tccacctgag ctacaagcag
    55741 tgataaagat aagggcaaag gcaagtacta aaacagttaa gagtggtttg aatagtttca
    55801 taagaatctc ctttatagat atgagccgaa ttggctaaag tcaaagacgt ttaaaaattc
    55861 ctgggctcgt ttggtttgcg gattggtaaa gaaggcttga gctgttcctt cttcagcgat
    55921 tttcccttgg tcgaggaaga taatccggtc agcaatggct tgggcaaact gcatttcgtg
    55981 ggttactaaa atcatggtac ggccttcttg ggccaaatca ttgataagtt ccagcacctc
    56041 acgcaccatt tctggatcca gcgaagcagt cacctcgtca aaaaggatga tttctggatg
    56101 cattaggagg gcacggacaa ttgcaacacg ttgcttctgt ccaccagata attgacgggc
    56161 aaagctatgt tgtttatcca gcaaaccgac acgatctagt aattgcaaag cttcttccgt
    56221 tacttctttc ttgtcccttc cttgagtttt gatagggcct aggatgaggt tttgtaagac
    56281 atccagatgg ggaaagagtt cataactttg aaagaccatg ccaatctttt ggcgaactag
    56341 gtgaaaatct tttttatttt caacgataga ctgaccatcc agaaaaatat ctccaccttg
    56401 aatactttct aagccgttga ggcaacgaag gagggtactt ttcccacaac cagatggccc
    56461 taggatgaca acaacttccc ttttttgatt tctagagaaa gtcctttgag gatgggattg
    56521 tctccgaagg atttttttag ttccttgatt tctaagatag tttcagacat ttagtttctc
    56581 caatgttttt ctaagtgagt ggatagtttg gaaatagggt agcaaactgc gaaatataag
    56641 actagaatgg ttccataaat ccaaaatgaa gcagttggga tggtcaggcg attgctatcg
    56701 atgatttgtt gtccaacttt ggtcacttcc acaaccccaa tcaaaacaac taatgaagtg
    56761 gttttaatca tccgagtgac aagattgata gcctgcggta gcagtcttct taagacttgt
    56821 gggatgatga tgtggtagta aagttgaaca ttagtcaagc cgagtgcctg tccactttca
    56881 aactgatgtt tagggagaga agtgatagct ccacgtacca agtctcccat ttcagctgtt
    56941 ccccagaggg taaaaacgat aatagctgaa gtctcacctg agatattgat attaaagttt
    57001 cgagccaagc caaagtaaac gatgaagagt agcaccagct ggggcatgat acggataaat
    57061 tccagataca atcgtgttaa aaatcgtatg attctagaat gggaggtcat gatgattccc
    57121 atgactgttc tgaacatcat ggataagagg acagacagga tggatatctc aatcgtaacg
    57181 cccaatccct gtaagattct caggagatta tttccctgaa agagtacttg gattcccgaa
    57241 tcctgcatgg cggagcctcc tttctatcca gctaaagacc agtgagatgg gtagcagcat
    57301 gatcagataa gcaactacca acatagctag cgcaatgtct gtctcatagt agagaccaat
    57361 caaatccttg gcgacataca tgaggtcggc caaagccact gctgagaaaa cagaggtttc
    57421 cttgataagg aaaatgacat tggcactaaa ggaggggagt gccaccgctg ttgcttgcgg
    57481 aagaaccaca tagcgaaaga cctgtagagg tgtcagacca atagcgaggc caatctcctg
    57541 ctgggtttga ctgatggctt ccagcccact tcggaaagat tctgccatat aggatcctcc
    57601 taaaaagaca agcccaagcg ttgcacagac ttctgaagat aggacaatcc cgattcgggg
    57661 aagaccgaag tagagaaaga agagttgaat caaaaggggc gtattacgtg acaattcaat
    57721 gtaggctgtc gctacttgcg ccaaaacagg gattcgataa tgtcggatga tactaacgat
    57781 taaaccgagc agaaaggatc ccaaaattcc ccaaactgca atatgcaagg tcagaaagaa
    57841 tgccttttga tatagtggta gatattgttc aacaatggac caatccaaaa atagaacctc
    57901 ccatctagaa ataatacagt tattgtagca cttaaaatct tctttggata atatctattt
    57961 tttattgccg ttataaggat tttttaccat agacataaaa tttctgaaat ttccaaacaa
    58021 aatatttgaa aagttttgaa aaagagttaa gatatttttg taatatacaa agtgaacgct
    58081 tacttattaa ggagggcatt ttatgtcata caaaacaagc aatgcagaag gtcatgtaga
    58141 tttcatcaat acctatgatt tggagccaat ggcgcaacaa gttattccta aagcagcatt
    58201 tggctatatc gctagtgggg cggaagatac tttcacttct ttccagtgat tttagcgtca
    58261 ggttcttttt agtttttaaa gattatccgt gaatttcttg cttatttatg ataaaatggg
    58321 ggtatcgcaa aaaatgactc atcgtattca attttgagta aaactaggag gatcccatgt
    58381 ctacagaaca tatggaagaa ctaaatgacc agcagatcgt tcgccgtgaa aaaatggctg
    58441 cgctctgcga acaaggaatc gatcctttcg gaaaacgttt tgaacgtact gcaaattcac
    58501 aagaattaaa agataaatat gccaacctcg ataaagaaca attacacgat aaaaacgaaa
    58561 cagctactat cgcaggacgc ttggtaacca aacgtggtaa aggaaaagtt ggttttgccc
    58621 accttcaaga ccgcgaaggc cagattcaga tctacgttcg taaggatgct gtcggtgaag
    58681 aaaactacga aatcttcaaa aaagcagacc ttggtgactt ccttggtgtc gaaggtgaag
    58741 tgatgcgtac ggatatggga gaactctcta tcaaggcaac ccacatcaca cacttgtcta
    58801 aggctcttcg tcctcttcct gagaaattcc atggtttgac agacgttgaa acaatttacc
    58861 gtaaacgtta ccttgacttg atttctaacc gtgaaagctt tgaacgcttt gtcactcgtt
    58921 caaaaatcat ctctgaaatc cgtcgttacc ttgaccaaaa aggattcctt gaagtggaaa
    58981 cacctgttct tcataatgaa gccggtggtg ctgctgcccg tccatttatc acccaccaca
    59041 atgcccaaaa cattgacatg gtgcttcgta tcgcgactga gcttcactta aaacgcctta
    59101 tcgtgggtgg tatggaacgt gtctatgaaa ttggccgtat cttccgtaac gaaggaatgg
    59161 acgctactca taaccctgag tttacttcta tcgaagttta ccaagcttat gcagacttcc
    59221 aagacatcat ggacttgacg gaaggcatta tccaacacgc tgctaaatca gttaagggcg
    59281 atggcccagt caactaccaa ggtactgaaa tcaagattaa cgaaccattt aagcgtgttc
    59341 atatggtgga tgctatcaga gaaattactg gtgtcgattt ctggcaagac atgactttag
    59401 aagaagctaa agttatcgct gctgagaaga aagttccagt tgagaaacac tacactgagg
    59461 ttggtcacat catcaatgcc ttctttgaag agtttgttga agaaacttta atccaaccaa
    59521 cctttgtcta tggacatcca gtagctgtat ctccactcgc taagaaaaat cctgaagacc
    59581 aacgctttac tgaccgtttc gagctcttta tcatgactaa ggagtacggt aatgccttta
    59641 ctgaactcaa cgacccaatc gaccaactta gccgttttga agcccaagct aaagccaaag
    59701 aacttggtga tgatgaagcg acaggaatcg actatgacta cattgaagct cttgaatacg
    59761 gtatgccacc aacaggtggt ttgggaatcg gtatcgaccg tctctgcatg ctcctcactg
    59821 atacaacaac tatccgtgat gtattgctct tcccaacaat gaaataaatt cttatcctct
    59881 ggctcttacc agaggatttt ttgattcaaa aagagactga atttaaggag aaaatgaagt
    59941 gtagtatatt gaagttgaaa tagtacactt tgatttctaa gacattgtta gaaattggtt
    60001 taaattccct aagcaatttg tgcatgtttt atttcatttt acgatagtac gctgaaactg
    60061 ttcaaaaagt actagaaatt gacttggatt ccccaattga tttgttcaga ttcactataa
    60121 ataaaaaatt aataagtggg ataggaagtt agcgtcaact aggatagtat cttgcttaaa
    60181 cagtatatat gggactgata taagtccata ggtcctatta gaggatgttc tggtgtctta
    60241 ttcacttgtt ttttatagta ttagtagata gaatcagcaa ataaaaaagt agttcaaatg
    60301 cctggcagca aaaaatctca acgattcact taatatagac taaggtttag agtttaagga
    60361 aagtagggct agtatattgt atgaaaaaat aagatggttt ttaagcaaaa ctcttgaaaa
    60421 tgattgaaaa aagagttatt atagttgtat agtaaaaagc aaacgcttac ttaatcgagg
    60481 aggacagttt atgtcataca aaacaagcaa tgcagaaggt catgtagact tcatcaatac
    60541 ctatgatttg gagccaatgg cgcaacaagt tattcctaaa gcagcatttg gctatatcgc
    60601 tagtggggcg gaagatactt tcactttaag agagaatatc cgtgccttta accacaagct
    60661 catcgttcct catacacttt gcaatgtaga aaatccaagt acagagattg aatttgcagg
    60721 tgaaaaacta tcttcaccaa tcattatggc gcctgttgcg gcccataaat tagcgaatga
    60781 acagggggaa gtggcgactg cgcgtggtgt gcatgagttt ggttctcttt atacaaccag
    60841 ttcttactct actgttgacc ttccagagat ttctgaagcc cttcaaggga cacctcattg
    60901 gtttcaattt tactttagta aggatgacgg tatcaaccgc cacatcatgg accgtgtgaa
    60961 ggctgaaggt tataaagcga ttgtcttgac ggcagatgct actgtagggg ggaatcgtga
    61021 agtggataag cgtaatggtt ttgtcttccc agttggcatg ccgattgttg aagaatacct
    61081 gccagaaggt gctggtaaat caatggactt tgtttacaaa tcagctaaac aacgcttgtc
    61141 tccacgcgat gtagaattta tcgctgaata ctctggtctt cctgtgtatg tcaagggacc
    61201 acaatgccgt gaggacgttg aacgttcgct tgctgcagga gcttctggta tctgggtaac
    61261 caaccacggt ggtcgtcaaa tcgacggtgg accagctgcc tttgactcgc ttcaagaagt
    61321 ggcagaagca gttgatagac gtgtgccgat tgtctttgac tctggtgttc gtcgtggtca
    61381 acacgtcttt aaagccttgg cttcaggagc agacttggta gctattggtc gccctgttat
    61441 ttatggcttg gctctcggtg gtagtgtcgg tgtgcgtcaa gtctttgaac acttgaatgc
    61501 ggaattgaag acagttatgc aattgtctgg agctcagacc attgaagatg tcaaacactt
    61561 taagctccgt cacaatccat acaacccaac cttcccagtt gaccctcgtg acttaaaatt
    61621 gtattgataa aacagattgc ctccactgaa tgtggaggtt tttcttcgtg tattggaaga
    61681 ataaattgct atcgaaaact atagtaaaat gaaataagaa taggacaaat caatcaggac
    61741 agtcaaatcg atttctaaca atgttttaga agtagaggtg tactattcta gtttcaatct
    61801 actatataaa gatatttttc ggtcaaatat cttgctaggg ctttcatttt ttgctatact
    61861 ggttcagtaa ttaaataata gagacatttg gggtgcttta agctgagatg atacccattg
    61921 aacctgatac agttaagact ggcgaaggga aatgtgaaac gttttttcgt atggcgaaat
    61981 gaacgatcta atcttgtaag tcaactctaa ttcttgattg taattggagt tttttgttta
    62041 tataaactgg ataggcttgt ttagttagct ctctgcaaat gctctcgatg tgctttaaaa
    62101 ggatcaagtg gaattacgat tttgcttgat atttcttggt gttttattaa ttactaacaa
    62161 gtaaagagag gagaagtaaa tatggaaaca caagactatg catttcagcc aggtttgact
    62221 gttggagaat tattaaaaag cagtcagaag gattggcagg ctgcaatcaa tcatcgtttt
    62281 gttaaggaac tttttgcggg gacaattgag aataaggtct taaaggacta tctgattcaa
    62341 gattatcact tctttgatgc cttcttatcc atgctgggtg cctgcgtagc tcacgcagat
    62401 aaacttgaat ccaagcttcg ttttgccaag caactaggct ttcttgaagc agatgaagat
    62461 ggttatttcc aaaaggcttt taaagagtta aaagtagcag agaatgacta tctagaagtg
    62521 actttgcacc ctgtaacaaa agcctttcag gatctaatgt attcggcagt ggcttcatca
    62581 gactatgccc atcttttggt tatgctggtc attgcagaag gtctctattt agactggggt
    62641 tctaaagatt tggctctacc tgaagtctat attcattcgg aatggatcaa tctccacaga
    62701 ggtcctttct ttgcagagtg ggttcaattt ctggttgacg aactcaatcg tgtcggtaaa
    62761 aatcgagaag atttgacaga acttcagcaa cgctggaatc aagcagtcgc tttagaatta
    62821 gctttttttg atattggtta cgatgtctag agaaggctca tgatatttac aaatttgatg
    62881 aggtaatact cttcgaaaat ctcttcaaac cacgtcagtt tcaccttgcc gtaggtatgg
    62941 ttactgactt cgtcagttct atccacaacc tcaaaacagt gttttgagct gacttcgtca
    63001 gtcttatcta caggttcaaa gcagtgtttt gagcaatctg cggttagctt cctagtttgc
    63061 tctttgattt tcattgagta taaatcattc aacgtaaatg aaaaaaattt cttaacgaaa
    63121 gatagagata aatcgaaatc agtagatcgt ataaagtgaa aaggaaggat ttcaatatga
    63181 caagtttaaa attattaaaa aaaaagcacc attggtcatt tgtataacca atgatgtagt
    63241 aaaaaatttc acagcaaatg gattagtagc actgggcgcc tcaccagcca tgagtgagtt
    63301 tccagcagat ttagaggatt tgttaaagta tgctggtggt ttattaataa acataggaac
    63361 attgacagat gaaaattgga aattatacca agctgctctg aaaattgcgg agaaatataa
    63421 tgtcccagca gttttagatc ctgtagcctg tggagcagga gaatatagaa aaaaagtagc
    63481 agatgatcta atcaacaatt ataaactagc agcgattaga ggaaatgctg gcgagattgc
    63541 ctctttagta ggaatagatg tggcatctaa aggagtagat agtgcgggcg tagataatat
    63601 tgacgaaatt gctctagcag caaatgagaa gttcaatatt ccaatagtag taacaggtga
    63661 agtggatgtc attgctgtta atggagaagt ggtaacgatt cataatggta gtgctatgat
    63721 gccgaaagtc attgggacag gatgcttatt aggagctgta gtagcaagtt ttatcggact
    63781 ggaaaaaggt caagaattaa aatcattaga aacagcaatg ttagtttaca atatcgctgg
    63841 agaaatggca gaaaaacgtc caaatggaca tcttcctggg acatttaaag ttgaatttat
    63901 aaatgcctta tacgagatta cagatgaaga tgtaaaggaa ttcaaaagag tgaagtaaga
    63961 tgtttcataa agaattacta aaactatatt ttatttgtgg aacgactact tgccaaggaa
    64021 aaaatctata tacagtcgtt gaggaagcct taaaaggtgg tataacctta tttcaatttc
    64081 gagaaaaagg tgagggtgct ttagaaggct tagaaaaact tgagctagct atccaaatta
    64141 aagaactttg taaaaaatac aatgttccat ttatcgttaa tgacgatata gatttagcaa
    64201 tggaaattga cgccgatggc gtacatgtag gtcaagatga tattggtgtt gatgaaatta
    64261 gaaaattgat gccagataaa ataattggtc tttctataag aaacgaagaa gaatttcaac
    64321 aatcaaaagt tgaatatgta gattatgtag gtgttgggcc tgtatttgat acccagtcaa
    64381 aagatgatgc tggtggtgct ataggttatg aaggtcttga attgatgaga aaactattgc
    64441 cacaaatgcc tttagttgca attggtggga tacagacgaa acatattaaa gacattataa
    64501 agaccaatat ggatggcgtt tcaatcattt cggcaatatc gtatgcaaaa aatatagaaa
    64561 aaactgttcg ggaaatgagt gaacaataga tatctatcct tggattctaa gtaagtagga
    64621 ttttcctttt ttaaaaagct atgtccagat tcagcttgaa tggctaattc atcaaacagg
    64681 tcatttgttt tttgcaaaag aaagatctgc tgtctttgtg agtgcaaggc tgtatttcca
    64741 gttcaatttc ttgaactagt ataaaatgtg tgttataata atcacataat taaataataa
    64801 agacatttgg ggtgctttag gctgagatga tacccattga acctgataca gttaagactg
    64861 gcgaagggaa atgtgaaatg tttttttcgt atgtcgaaaa gaactgtcta atcttgtaag
    64921 ctgaactcta attcgtatct gtaattagag ttttttcttt tacgaaactg gatgaagatg
    64981 atacagtaga ttgaaataag atgcgaacaa atcaattaga aaattcaaat taatttctag
    65041 aaatatttta gtgttcttgt gtactgttct aaattcagtc tgctatacaa agtatttagg
    65101 tcggtcttct tgagtgcccc acttttcttt aaaaaaagga gttttttatg ttgaaaaaat
    65161 ggcagttaaa agatgttatc ttgcttgctt tcttgtctat cttttttggt ggggttttcg
    65221 ttggttcagg atatgtgtat aatattctca gtctactctt aacacctctt ggtttgcagg
    65281 cctttgccaa tgaaatcctc ttcggtctct ggtgtatggc tgcgcccatt gctgccatct
    65341 ttgttccgag agtcggaagt gcaacgattg gagaagtgct agctgcgctt gctgaagtcc
    65401 tttatggtag ccaatttggt ctaggagctc ttttgtctgg cttggttcaa ggtttgggaa
    65461 gtgaatttgg ttttatcgta actaagaatc gctatgaaag ttggctctct ctaactgcta
    65521 atagtattgg gattacgctt gttagctttg tctatgaata cattaagtta ggttactacg
    65581 ccttttccct tccgtttgtc ctttccttgc ttgtggtacg ttttatttct gtttatttct
    65641 tctgtaccat cttggttcgt gccattgtca aactctatca tcagtttgca actggaggaa
    65701 aagcatagat gggtctggaa ctacgagcga ttcagtcccc aatcttctct gagccgtttg
    65761 attttacttt tcatgcgcaa gcctttacct tgttagttgg gagcagtggt tcaggaaaat
    65821 ccagcctctt tcaaatgatt gcccaagtca gttctcttcc ctatagtggt caagtcctga
    65881 tagatgggag cgaggtcagt cagctttcta tcatcgcacg tgtccagaag gttggcattc
    65941 tcttgcaaaa tcccaatcat caatttacca tggagaactt gtttgaggag ttgattttca
    66001 ccttggagaa tatcggctat caccttcagg aaattgattc taaaatagca gaggttgtcc
    66061 agcaatgtcg ttgcgaggca atcttacacc gtcccattca tcacctatca ggtggggaaa
    66121 agcaaaaagc agcgctggct gtcctctttg ccatgaatcc tagggtctat ctcttggatg
    66181 agcccttcgc ttccattgac cgcaagagta gaatcgagat attggagatt ctaaaagagt
    66241 tggctcttga tgggaagaca gtcattttgt gcgaccatga tttatctgac tataaaacct
    66301 atatcgacta tatggttgag ctaagagacg gaaaactaag ggaagtgttt caaatccctt
    66361 cctatgagat gacacaggtt gcttcaaagg aagttgctgc tagcccggaa ttattccata
    66421 tgaaccgtgt gactggtgag cttggtaatc gccccctctt ttcaattgct gatttcacat
    66481 tctatcaagg gatttcctgt atcctgggtg acaatggtgt cgggaaatca accctctttc
    66541 ggtctattct tcaatttcaa aagtataagg ggagcattgc atggaagggg acagtcctga
    66601 aaaagaaaaa gagtttgtat cgtgatctga ctggtgttgt tcaggaagct gagaagcagt
    66661 ttatccgagt cagtctgcga gaggagcttc aattagatgg acctgattct gaaagaaatc
    66721 agcggatttt tcaagcttta cgatattttg atttggagca ggcagtcgat aagagtccct
    66781 atcaattaag tggtggtcag caaaaaattc ttcagctctt gaccatcttg accattaagg
    66841 cttccgtgat cttgctggat gaaccttttg caggtttgga tgatagagcc tgccattatt
    66901 tttgcaagtg gattgtggag gagaggaatc aaggaagaac ttttctgctc attagtcatc
    66961 gtttagaccc tttgatttct gtggttgatt attggattga gatgactagt cagggtcttc
    67021 ggcatgtgaa agaagtgacc attaccaaac cacttacatc tcagagtagc aatacccaag
    67081 gggaggtgag atagtatggt caaagtagca acccagacac cgattatcag tctcttcttg
    67141 ctgattttat ccttggaaac atctttcatt ccttcgattg ctctgaatct ttcggtagtc
    67201 gcattttgta ttctctttat gctctattac cgtcgattta aaatgttagc ttggatgatc
    67261 atacttgcca ttttaccatc ttttgccaac tactgggcag ttcagttaca cggagatgct
    67321 tcacaggcag ttatgcttgg aacgagggcc tttgtgacag tttgtatcgg ccttgtcttt
    67381 gtttcctcta tttcactaaa agagcttctc ttgtacttgg ctcaaaaggg gctatcacgc
    67441 tcttggtcct atgccttgat tgtggtattc aattcctttc ctctcattca gcaagaaatc
    67501 aagtccctca aagaagcttg cctattacgt ggtcaagaac tacatttttg gtcgcccttg
    67561 atttacagta aggttctgat gacagtcttt aggtggcgcc atctttacct gagagctcta
    67621 tctgctcacg gatatgacga acatgcacag ttgaagaata gctatcggac tttttatatt
    67681 cctaaaaaaa caaaattaat ctacctgctt ttctttttat tgcttcaaac cagtctattt
    67741 ttataaagga gttattatgg aatttacaga tattgcgatg gaattatcca agaaagcttg
    67801 gcaggcttcc tttcatcatc cctttatttt acaattacaa gaggggaatt tagaacctgc
    67861 cattttccgc tattacctga ttcaggatgc ctactatctg aaggccttct cagaaatcta
    67921 tcatcttttg gctgataagg cttcaaacca agagatgaaa agactcttga aacaaaatgc
    67981 tcagggttta gtggagggtg agttatttat tcgccaacaa tttttcaagg aattggaaat
    68041 cagcgaccag gaaatggagc aacatccaat cgctccaacc tgttatcatt acatttctca
    68101 tatttatagg caatttgcag aaccgaactt agccatcgct tttgcaagct tgcttccttg
    68161 tccttggtta taccatgata taggcaaatc acttaatctt aaaccatcac caaatcctct
    68221 ctatcaacaa tggattgaaa cttatattac ggatgagtta gagcaacaga tcagagagga
    68281 gggagcactg gtcaatcagc tctatcgaga aagtgacgag acagacaagc aaaaaatgct
    68341 agatgccttc cacatcagtg ttcatatgga agccaagttt tgggaaatgg cctaccaaca
    68401 ccagacatgg aagagcgatt tacagtcttt agaaaaagga gaagaataga agcatgagaa
    68461 agcaccaatt acaagttcac aaattaacca ttttatctat gatgattgcc cttgatgtag
    68521 tccttacacc tatctttcga attgagggaa tggcaccgat gtccagtgta gtcaatattc
    68581 tagcgggaat catgatggga cctgtttatg ccttggccat ggctacagtc acagccttta
    68641 tccgtatgac gactcaaggg attccgcctt tagctctcac aggagcgact tttggagccc
    68701 ttctggcagg tctcttttat aagtacggtc gaaaatttta cttttctgcc ttgggagaaa
    68761 ttgtgggaac aggtattatt ggttccattg tttcctatcc tgttatggta ctctttacag
    68821 gatcagctgc taagcttagc tggtttatct acacgcctcg atttttcgga gcaactttga
    68881 ttggtacagc gatttccttt attgcctttc gatttttaat caagcaggaa ttctttaaaa
    68941 aagtgcaggg atatttcttt agtgaaagga tagactgatg caggaattta caaatccctt
    69001 tcctataggc tctagttccc taattcactg tatgaccaat gagatttctt gtgagatgct
    69061 ggcaaatggg attttggctc tgggatgcaa acctgtcatg gcagatgatc cccgtgaggt
    69121 tcttgatttt actaagcaaa gtcaggctct cttcatcaat ttggggcatt tgtcagctga
    69181 gaaggaaaaa gcaatccgca tggcagcttc gtatgcaaac caatcttctc tcccgatggt
    69241 agtagatgcg gttggcgtaa cgacttcatc cattcgtaag agcttagtta aagacctttt
    69301 agactataga cctacggtca ttaaaggaaa catgtcagaa attcgaagtc ttgttggatt
    69361 aaagcaccac ggcgttgggg tcgatgcgag tgctaaagat caagaaacgg aggatttgct
    69421 tcaagtcttg aaagactggt gtcagaccta tcctggtatg tctttcttag tcacaggtcc
    69481 caaggacctc gtcgtttcga aaaatcaggt cgctgtactg ggaaatggct gtactgaatt
    69541 agactggata acagggacag gagacttggt tggagcctta acagctgttt ttctcagcca
    69601 aggaaagact ggttttgaag cttcttgctt agcagtctct tatctcaata tcgctgctga
    69661 gaaaatagtt gttcaaggaa tgggattgga agaatttcgt taccaagtac tcaatcagct
    69721 ttcgctccta agaagagatg aaaattggct agataccatc aaaggagagg tttatgaata
    69781 gagaagcact tagactatat ctggtaacca atcgctatca agattccgtg gaaagctttc
    69841 ttgcaaaaat tgagacggcc tgccgttcag gggttaccat agtccaattg cgagaaaaaa
    69901 atctcacaac caatcaatat tatcaactgg caaaacaagt caaggaaata acagatgcct
    69961 atcaggtccc cttgataatc gatgatcggt tggatgtttg tcttgcggtt gatgcagcag
    70021 gtctgcatat cggagatgat gaattaccag tttcggtagc tcgcaaagtc ttgggtcctg
    70081 aaaaaatcct cggtgtcacc gctaaaacgg ttaaaagagc tctggaagcg gagaaatcag
    70141 gtgcagatta cttgggtaca ggagccattt tcccaaccac caccaaggaa aatgcgccca
    70201 ttactctgat atcgaccttg aaaacaattt gccaaacggt tgccattcca gtagttgcta
    70261 ttggaggctt gacttcggag aatattgatc agcttatggg cactggcata gctggtgtag
    70321 ctgttgtacg tgatttgatg caagcagaag atattgaggc aaaaacgcaa gcctttttaa
    70381 caaagttgca tgacattctt tcctaattaa tactcaatga aaatcaaaga gtaaactaga
    70441 aagctagtcg caggttgctc aaagtactgc cttgaggttt tagataagac tgacgaagtc
    70501 agctcaaaac agtgttttga ggttgcagat ggaagctgac gtagtttgaa gagattttcg
    70561 aagagtataa aaactctcta attcccttaa agtgggaact agagagtttt ttttcttaat
    70621 ctttaaaagt tgtatggttg actggaccag aaccatgacc gagttgaggg gcatcttgaa
    70681 tagcttttgt gataaaggcc ttggccttat caactgcctg gtaaagactc ttgcccttgg
    70741 ctagttcagc agtaatcact gcagcaaagg tacatccagt accatgggtg tgacaggttt
    70801 gaattcgtgg gctttcccag acaaattgtt cattcttggt aaagaggaaa tctttagcac
    70861 cacctttgag atgtccgcct ttgataacca cagactgagg accaaattct tttaaaatca
    70921 ggcgaccagc acgctgcatg tcttcggggt catggattga aaaaccaaca atctcttctg
    70981 cttcaggaag atttggcgta ataatagttg ctagaggtag taagtttgtt ttgagatagt
    71041 ctctagcact tgagtcaatc aaggcatctc cacttgtagc aaccataaca ggatcaagga
    71101 catagggaca atccagtttt ttaagatagg gttggatgat ttccatgatt tcagtagtag
    71161 ccaacattcc agtttttaca gcctgaggtg gaatatcaga aaagacactg tccaattggg
    71221 ctttcaacat ttgaggagaa acgtgttcga ttagctgaac acctctggta ttttgagcga
    71281 caagactggt tacaacagcc attccatagg catctctcgc ttggaatgac tttaaatctg
    71341 ccataatgcc agcaccacca ctagggtcag tccctgcaat ggtcaaagca acgggtaaat
    71401 aagtcatcta aaacctccca accaactgaa gtttgtattc ttaagaacaa gctagtctgt
    71461 tttagaaagg caatagaaga ctgctagtcc ccattatcat ttccacttcc atcagctagc
    71521 attacctaga ttgagtcaga gggatctaac agtcgttaat ctcagcttta cgcaccccta
    71581 gtggttgttt tcttttttct ttagtatagc atatttttat tgtttatata gtaatatttg
    71641 actgcaaatc gtttttcaaa tgattaaaat tctatttttc gaagaaaata ttatttctat
    71701 aattgacaaa tgtagatttt gagagtatac tgatgagtgt aattgacaaa tgtagatttt
    71761 ggaggtatgg taatgcagat ttcagatgca gaatggcagg tcatgaagat tatttggatg
    71821 cagggggagc agaccagtac agatttgatt agggttttgg cagagcggtt tgattggtcc
    71881 aagtcaacgg ttcagacact tttgtctcgg ctggttgaga aagagtgttt gactcggaaa
    71941 aaagaaggca agtcctttgt ttattcagcc cttttaactc tagaccaaag tcgggattta
    72001 cttgtccaag atatcaaaga caaggtttgt tcccgtagga ttaggaattt gttggctgac
    72061 ttgattgtag aatgtgaatt tactcagact gacttggaag acttggaagc tgtgatttca
    72121 gagaagaaat caagtgctgt aacagaagta agatgtaatt gtatgtaaag gagacgtcat
    72181 gttaaatagt attgtaacca ttatttgtat tgcccttatc gcgtttatct tgttttggtt
    72241 tttcaaaaag cctgaaaaat ctggacaaaa agcccagcaa aaaaatggat accaagagat
    72301 tcgagtggaa gtcatgggag gctatactcc tgagttgatt gtcctcaaga aatcagtgcc
    72361 agcacgcatt gtctttgacc gcaaggatcc ttcaccatgt ctggatcaaa ttgtttttcc
    72421 agattttggt gtacatacga acctgccaat gggggaagag tatgtagtgg aaatcacgcc
    72481 tgagcaggct ggagagtttg gctttgcttg tggtatgaac atgatgcacg gcaagatgat
    72541 tgtagagtag gtggagacta tgacagaaat tgtgaaagca agcttagaaa atggcattca
    72601 aaaaatccgt atccgagctg aaaaaggcta tcatccagcc catatccagc ttcaaaaggg
    72661 aattccagct gagattacct ttcatcgtgc tactccttca aactgttata aggaaattct
    72721 gtttgaagaa gaaggtatct tggaaccaat cggcgtagat gaggagaaag tcattcgttt
    72781 tacacctcaa gaattagggc gacatgaatt ttcttgtggc atgaagatgc aaaagggaag
    72841 ctatacagtc gttgagaaga ctcgaaaatc tctatctctc ctgcaacgtt tttggattac
    72901 tagtatcttt actgtgcctc ttgtgattct catgattggg atgttgacag gtagcattag
    72961 tcatcaagtc atgcattggg gaacctttct agcaacaacg actattatgt tagttgcagg
    73021 taagccatat atccagagtg cttgggccag ttttaaaaag cacaatgcca acatggatac
    73081 cttggttgcg ctgggaactc tagtggctta tttctatagc ctagttgctc tctttgctgg
    73141 tctccctgtt tacttcgaaa gtgctggatt tatcctcttt ttcgttcttt tgggagcagt
    73201 ttttgaggaa aaaatgagaa aaaatacgtc ccaagctgtg gagaaattac tggacttgca
    73261 agctaaaacc gcagaagtct tgagtgatga tagttatgtc caagttcctt tggaacaagt
    73321 caaggtaggc gacctgattc gagtgcgtcc cggtgaaaag attgctgttg atggtgtcgt
    73381 agtagaaggt gtctctagta ttgacgaatc catggtgaca ggtgagagtc tgcctgtgga
    73441 caagacagtt ggagatactg tcattggctc aaccatcaat catagtggaa cgcttgtctt
    73501 tagagcagaa aaagttggct cagagactgt tttggctcag attgtggatt ttgtgaagaa
    73561 agctcagaca agtcgtgcgc cgattcaaga cttgacggat aagatttcag ggatttttgt
    73621 cccagtagtt gtcattttag gaatcatgac cttttgggtt tggttcgtct tgctcaggga
    73681 tagtgtggtt gtgcttggag ctagctttgt gtcctctctt ctctacggag tggcggtttt
    73741 gattatcgcc tgtccttgtg ccttgggact tgcaacaccg acagccctta tggtggggac
    73801 aggacgtagt gccaagatgg gggttctcct caaaaatgga actgtcttac aggaaatcca
    73861 gaaagttcaa acccttgtct ttgataagac cgggactttg acggaaggga aacctgtggt
    73921 aacagatgtc atcggcgacg aagtagaagt gtttaggttg gcagcctcct tggaagatgc
    73981 ttctcaacac ccactggctg aggctatcgt taagcgagcg agtgaagctg gacttgagtt
    74041 tcaaactgtt gaaaattttc agaccttgca cgggaaaggt gtttcagggc gaatcaatgg
    74101 aaaacaagtt ttacttggaa atgctaaaat gctggatggc atggatattt ctaatactta
    74161 tcaagataaa ctagaagaac tagaaaaaga agctaagaca gttgtgttct tagctgttga
    74221 caatgaaatc aaaggcttgc ttgctttgca agatattcct aaggaaaatg ctaagctagc
    74281 catcagtcag ctaaaaaaac gtggtctccg aacagtcatg ctgacaggag acaatgctgg
    74341 tgtggcgcgt gctattgcag atcaaatcgg aattgaagag gtcattgcag gcgtcttgcc
    74401 agaagaaaaa gcccatgaaa tccataaact gcaacagtca gggaaagtag cctttgttgg
    74461 ggacggtatc aatgacgctc ctgcccttag tgtagcagat gtggggattg ctatgggagc
    74521 tggaacagat atcgccatcg agtcagcaga tttggtgttg acaaccaata atcttttagg
    74581 agtggttcgt gcctttgata tgagtaagaa aacctttcat cgaattctac tcaatctttt
    74641 ctgggctttt atctacaatg ttgtcggaat tccgattgca gcaggagtct tttcaggtgt
    74701 tggactggct ctcaacccag aattggcagg tctagccatg gcctttagtt ctgtatctgt
    74761 tctgaccagt tcccttctgt taaattttag taaaatagac taaaagtaat gactagattt
    74821 ctttgttata aaacagaaat gacagaactt tcaaaactgt attagaatag agaatagaga
    74881 gttttgagca gatttttaga aaagtcagca taaatatgat acagtggaat agtaaaaatt
    74941 tggagaacgt ttccaattct atgtaatcgt attctccaaa tctaaaaaaa ttgaaggaga
    75001 gttatcatta tgactcaagg gaaaattact gcatctgcag caatgcttaa cgtattgaaa
    75061 acatggggcg tagatacaat ctacggtatc ccatcaggaa cactcagctc attgatggac
    75121 gctttggctg aagacaaaga tatccgcttc ttacaagttc gccacgaaga gacaggtgct
    75181 cttgcagcgg ttatgcaagc taaattcggc ggctcaatcg gggttgcagt tggttcaggt
    75241 ggtccaggtg cgactcactt gattaacggt gtttacgatg cagctatgga taacactcca
    75301 ttcctagcga tccttggatc acgtccagtt aacgaattga acatggatgc tttccaagag
    75361 cttaaccaaa acccaatgta caacggtatc gctgtttaca acaaacgtgt agcttacgct
    75421 gagcaattgc caaaagtaat tgacgaagcc tgccgtgctg caatttctaa aaaaggtcca
    75481 gctgttgttg aaattccagt aaacttcggt ttccaagaaa tcgacgaaaa ctcatactac
    75541 ggttcaggtt catacgaacg ctcattcatc gctcctgctt tgaacgaagt tgaaatcgac
    75601 aaagctgttg aaatcttgaa caatgctgaa cgcccagtta tctatgctgg atttggtggt
    75661 gttaaagctg gtgaagtgat tactgaattg tcacgtaaaa tcaaagcacc aatcatcaca
    75721 actggtaaaa actttgaagc tttcgaatgg aactatgaag gtttgacagg ttctgcttat
    75781 cgtgttggtt ggaaaccagc caacgaagtg gtctttgaag cagacacagt tcttttcctt
    75841 ggttcaaact tcccatttgc tgaagtttac gaagcattca agaacactga aaaattcatc
    75901 caagttgata tcgaccctta caaacttggt aaacgtcatg cccttgacgc ttcaatcctt
    75961 ggtgatgctg gtcaagcagc taaagctatc cttgacaaag taaacccagt tgaatcaact
    76021 ccatggtggc gtgcaaacgt taagaacaac caaaactggc gtgattacat gaacaaactc
    76081 gaaggtaaaa ctgagggtga attgcaattg tatcaagttt acaatgcaat caacaaacat
    76141 gctgatcaag acgctatcta ctcaatcgac gtaggtaaca ctactcaaac atctactcgt
    76201 caccttcaca tgacacctaa gaacatgtgg cgtacatctc cactctttgc gacaatgggt
    76261 attgcccttc ctggtggtat cgctgctaag aaagacaatc cagatcgcca agtatggaac
    76321 atcatgggtg atggagcatt caacatgtgc tacccagacg ttatcacaaa cgttcaatac
    76381 gaccttccag ttatcaacct tgtcttctca aatgctgagt acggcttcat caagaacaaa
    76441 tacgaagata caaacaaaca cttgtttggt gtagacttca caaacgctga ctacgctaaa
    76501 attgcggaag ctcaaggagc tgttggattc acagttgacc gtatcgaaga catcgatgca
    76561 gttgttgcag aagctgttaa attgaacaaa gaaggtaaaa ctgttgtcat cgatgctcgc
    76621 atcactcaac accgtccact tccagtagaa gtacttgaat tggatccaaa acttcactca
    76681 gaagaagcta tcaaagcctt caaggaaaaa tacgaagcag aagaactcgt accattccgt
    76741 ctcttcttgg aagaagaagg attgcaatca cgcgcaatta aataattcct ctcgccgaaa
    76801 atcaaatatg aaacttgtag actttattct ttgattttca tagagcagaa ctttaaaaga
    76861 tcggagcaat ccggtctttt tatggaggag agtaaatgaa tttaaatcaa ttagatatta
    76921 tcgtttcaaa tgttccccaa gtctgtgctg acttggagca tattttggat aaaaaggcag
    76981 attatgctga tgatggtttt gctcagttta cgattggtag tcactgcctt atgttgtcac
    77041 aaaatcattt ggttcctttg gaaaactttc agtcaggaat cattattcat atcgaggttg
    77101 aggatgtaga ccagaactac aaacggttga acgagcttgg tataaaggtt ttacacggac
    77161 caactgtaac cgattgggga acagagtcct tattagttca aggacctgct ggtctagtgc
    77221 ttgattttta tcgtatgaaa taggtcgcgt agtcatttga cgttaacctt agctattttt
    77281 aaaatagaaa ttgagaatat gaactattga aagatcggag caatccggtc tttttatata
    77341 aagaaattgc atataatact catctgcttt caaaaagcat tctagtccat ctccgattaa
    77401 cgatggactt tatcacctcc ttctccagtc cttgtatgac atcttg
//
LOCUS       DAVKJJ010000002        67007 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000002 DAVKJJ010000000
VERSION     DAVKJJ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 67007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67007
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.002"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(196..273)
                     /locus_tag="VW144_000075"
                     /pseudo
     CDS             complement(196..273)
                     /locus_tag="VW144_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            344..1000
                     /locus_tag="VW144_000076"
     CDS             344..1000
                     /locus_tag="VW144_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEU9599587.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            990..1313
                     /locus_tag="VW144_000077"
     CDS             990..1313
                     /locus_tag="VW144_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEU9599588.1"
                     /translation="MVSKYLLLAVIFSGLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            1416..2246
                     /locus_tag="VW144_000078"
     CDS             1416..2246
                     /locus_tag="VW144_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9599589.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2400..3254
                     /locus_tag="VW144_000079"
     CDS             2400..3254
                     /locus_tag="VW144_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9599590.1"
                     /translation="MKIKKWLGLVALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            3354..4727
                     /locus_tag="VW144_000080"
     CDS             3354..4727
                     /locus_tag="VW144_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU9599591.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            4720..5781
                     /locus_tag="VW144_000081"
     CDS             4720..5781
                     /locus_tag="VW144_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEU9599592.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            5783..6475
                     /locus_tag="VW144_000082"
     CDS             5783..6475
                     /locus_tag="VW144_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9599593.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(6505..7074)
                     /locus_tag="VW144_000083"
     CDS             complement(6505..7074)
                     /locus_tag="VW144_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU9599594.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            7209..7823
                     /locus_tag="VW144_000084"
     CDS             7209..7823
                     /locus_tag="VW144_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9599595.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLVGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKILLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            7825..9471
                     /locus_tag="VW144_000085"
     CDS             7825..9471
                     /locus_tag="VW144_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9599596.1"
                     /translation="MKHFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHMGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVDSIQAIAQGQEGLRIDTLEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQKYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            9483..10769
                     /locus_tag="VW144_000086"
     CDS             9483..10769
                     /locus_tag="VW144_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9599597.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            10821..11354
                     /locus_tag="VW144_000087"
     CDS             10821..11354
                     /locus_tag="VW144_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9599598.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            11432..11902
                     /gene="nrdI"
                     /locus_tag="VW144_000088"
     CDS             11432..11902
                     /gene="nrdI"
                     /locus_tag="VW144_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEU9599599.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(12195..13457)
                     /locus_tag="VW144_000089"
     CDS             complement(12195..13457)
                     /locus_tag="VW144_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEU9599600.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(13767..14225)
                     /locus_tag="VW144_000090"
     CDS             complement(13767..14225)
                     /locus_tag="VW144_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEU9599601.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(14222..14662)
                     /locus_tag="VW144_000091"
     CDS             complement(14222..14662)
                     /locus_tag="VW144_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9599602.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            15018..16967
                     /gene="mutL"
                     /locus_tag="VW144_000092"
     CDS             15018..16967
                     /gene="mutL"
                     /locus_tag="VW144_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU9599603.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(17292..17759)
                     /locus_tag="VW144_000093"
     CDS             complement(17292..17759)
                     /locus_tag="VW144_000093"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU9599604.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(17760..18965)
                     /locus_tag="VW144_000094"
     CDS             complement(17760..18965)
                     /locus_tag="VW144_000094"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU9599605.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(18985..19620)
                     /gene="ribE"
                     /locus_tag="VW144_000095"
     CDS             complement(18985..19620)
                     /gene="ribE"
                     /locus_tag="VW144_000095"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU9599606.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKKLLYQNGF"
     gene            complement(19605..20705)
                     /gene="ribD"
                     /locus_tag="VW144_000096"
     CDS             complement(19605..20705)
                     /gene="ribD"
                     /locus_tag="VW144_000096"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU9599607.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHYVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(20840..20954)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            21111..21704
                     /gene="ruvA"
                     /locus_tag="VW144_000097"
     CDS             21111..21704
                     /gene="ruvA"
                     /locus_tag="VW144_000097"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU9599608.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            21714..22277
                     /locus_tag="VW144_000098"
     CDS             21714..22277
                     /locus_tag="VW144_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU9599609.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTETELEAMLENPAIIRNRAKLFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            22274..22951
                     /locus_tag="VW144_000099"
     CDS             22274..22951
                     /locus_tag="VW144_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9599610.1"
                     /translation="MMSNKNKEILIFAILYTVLFMFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(23106..24137)
                     /locus_tag="VW144_000100"
     CDS             complement(23106..24137)
                     /locus_tag="VW144_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEU9599611.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGRAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(24392..25075)
                     /locus_tag="VW144_000101"
     CDS             complement(24392..25075)
                     /locus_tag="VW144_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEU9599612.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(25087..26031)
                     /locus_tag="VW144_000102"
     CDS             complement(25087..26031)
                     /locus_tag="VW144_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9599613.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            26163..28994
                     /gene="uvrA"
                     /locus_tag="VW144_000103"
     CDS             26163..28994
                     /gene="uvrA"
                     /locus_tag="VW144_000103"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU9599614.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPEGQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVSEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            28987..30048
                     /locus_tag="VW144_000104"
     CDS             28987..30048
                     /locus_tag="VW144_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9599615.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLDLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            30180..30578
                     /gene="spx"
                     /locus_tag="VW144_000105"
     CDS             30180..30578
                     /gene="spx"
                     /locus_tag="VW144_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEU9599616.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            30644..31213
                     /locus_tag="VW144_000106"
     CDS             30644..31213
                     /locus_tag="VW144_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9599617.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            31300..31566
                     /locus_tag="VW144_000107"
     CDS             31300..31566
                     /locus_tag="VW144_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEU9599618.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            31570..31989
                     /gene="ruvX"
                     /locus_tag="VW144_000108"
     CDS             31570..31989
                     /gene="ruvX"
                     /locus_tag="VW144_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU9599619.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            32005..32310
                     /locus_tag="VW144_000109"
     CDS             32005..32310
                     /locus_tag="VW144_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEU9599620.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            32568..33818
                     /locus_tag="VW144_000110"
     CDS             32568..33818
                     /locus_tag="VW144_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9599621.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGALKRKD
                     YQGMLGYLTEKLPQVELKVTGFDYQGALDERDVTGYDIVSSYREFISDFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            33815..34360
                     /locus_tag="VW144_000111"
     CDS             33815..34360
                     /locus_tag="VW144_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEU9599622.1"
                     /translation="MTPLFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDID
                     GTYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVN
                     GNQIVVDDMDRDPSDQIVLTK"
     gene            34506..36038
                     /gene="cls"
                     /locus_tag="VW144_000112"
     CDS             34506..36038
                     /gene="cls"
                     /locus_tag="VW144_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEU9599623.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            36297..37835
                     /locus_tag="VW144_000113"
     CDS             36297..37835
                     /locus_tag="VW144_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9599624.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            37955..40162
                     /gene="nrdD"
                     /locus_tag="VW144_000114"
     CDS             37955..40162
                     /gene="nrdD"
                     /locus_tag="VW144_000114"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEU9599625.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKQEDYAWK
                     KAQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            40174..40314
                     /locus_tag="VW144_000115"
     CDS             40174..40314
                     /locus_tag="VW144_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9599626.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            40358..40864
                     /locus_tag="VW144_000116"
     CDS             40358..40864
                     /locus_tag="VW144_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9599627.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKFQSPHDGGFWDTENFVYEDWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            40857..41447
                     /gene="nrdG"
                     /locus_tag="VW144_000117"
     CDS             40857..41447
                     /gene="nrdG"
                     /locus_tag="VW144_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEU9599628.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            41444..42028
                     /locus_tag="VW144_000118"
     CDS             41444..42028
                     /locus_tag="VW144_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEU9599629.1"
                     /translation="MKKKDLVDQLVSAIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVD"
     gene            42328..42636
                     /gene="rpsJ"
                     /locus_tag="VW144_000119"
     CDS             42328..42636
                     /gene="rpsJ"
                     /locus_tag="VW144_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU9599630.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            42853..43479
                     /gene="rplC"
                     /locus_tag="VW144_000120"
     CDS             42853..43479
                     /gene="rplC"
                     /locus_tag="VW144_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU9599631.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            43504..44127
                     /gene="rplD"
                     /locus_tag="VW144_000121"
     CDS             43504..44127
                     /gene="rplD"
                     /locus_tag="VW144_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU9599632.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            44127..44423
                     /locus_tag="VW144_000122"
     CDS             44127..44423
                     /locus_tag="VW144_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU9599633.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            44441..45274
                     /gene="rplB"
                     /locus_tag="VW144_000123"
     CDS             44441..45274
                     /gene="rplB"
                     /locus_tag="VW144_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU9599634.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            45378..45659
                     /gene="rpsS"
                     /locus_tag="VW144_000124"
     CDS             45378..45659
                     /gene="rpsS"
                     /locus_tag="VW144_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU9599635.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            45671..46015
                     /gene="rplV"
                     /locus_tag="VW144_000125"
     CDS             45671..46015
                     /gene="rplV"
                     /locus_tag="VW144_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU9599636.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            46028..46681
                     /gene="rpsC"
                     /locus_tag="VW144_000126"
     CDS             46028..46681
                     /gene="rpsC"
                     /locus_tag="VW144_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU9599637.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            46685..47098
                     /gene="rplP"
                     /locus_tag="VW144_000127"
     CDS             46685..47098
                     /gene="rplP"
                     /locus_tag="VW144_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU9599638.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            47108..47314
                     /locus_tag="VW144_000128"
     CDS             47108..47314
                     /locus_tag="VW144_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU9599639.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            47339..47599
                     /gene="rpsQ"
                     /locus_tag="VW144_000129"
     CDS             47339..47599
                     /gene="rpsQ"
                     /locus_tag="VW144_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU9599640.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            47625..47993
                     /gene="rplN"
                     /locus_tag="VW144_000130"
     CDS             47625..47993
                     /gene="rplN"
                     /locus_tag="VW144_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU9599641.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            48071..48376
                     /gene="rplX"
                     /locus_tag="VW144_000131"
     CDS             48071..48376
                     /gene="rplX"
                     /locus_tag="VW144_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU9599642.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            48400..48942
                     /gene="rplE"
                     /locus_tag="VW144_000132"
     CDS             48400..48942
                     /gene="rplE"
                     /locus_tag="VW144_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU9599643.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            48960..49229
                     /gene="rpsN"
                     /locus_tag="VW144_000133"
     CDS             48960..49229
                     /gene="rpsN"
                     /locus_tag="VW144_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEU9599644.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            49515..49913
                     /gene="rpsH"
                     /locus_tag="VW144_000134"
     CDS             49515..49913
                     /gene="rpsH"
                     /locus_tag="VW144_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU9599645.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            50105..50641
                     /gene="rplF"
                     /locus_tag="VW144_000135"
     CDS             50105..50641
                     /gene="rplF"
                     /locus_tag="VW144_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU9599646.1"
                     /translation="MSRIGNKVIVLPAGVELTNNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEVTLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            50725..51081
                     /gene="rplR"
                     /locus_tag="VW144_000136"
     CDS             50725..51081
                     /gene="rplR"
                     /locus_tag="VW144_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU9599647.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            51099..51593
                     /gene="rpsE"
                     /locus_tag="VW144_000137"
     CDS             51099..51593
                     /gene="rpsE"
                     /locus_tag="VW144_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU9599648.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            51565..51789
                     /gene="rpmD"
                     /locus_tag="VW144_000138"
     CDS             51565..51789
                     /gene="rpmD"
                     /locus_tag="VW144_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU9599649.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSFVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            51934..52374
                     /gene="rplO"
                     /locus_tag="VW144_000139"
     CDS             51934..52374
                     /gene="rplO"
                     /locus_tag="VW144_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU9599650.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            52387..53697
                     /gene="secY"
                     /locus_tag="VW144_000140"
     CDS             52387..53697
                     /gene="secY"
                     /locus_tag="VW144_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU9599651.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            53848..54486
                     /locus_tag="VW144_000141"
     CDS             53848..54486
                     /locus_tag="VW144_000141"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU9599652.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAQALDKTLA
                     ELGIELEGVINIEVNPNSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            54603..54821
                     /gene="infA"
                     /locus_tag="VW144_000142"
     CDS             54603..54821
                     /gene="infA"
                     /locus_tag="VW144_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU9599653.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            54846..54962
                     /gene="rpmJ"
                     /locus_tag="VW144_000143"
     CDS             54846..54962
                     /gene="rpmJ"
                     /locus_tag="VW144_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU9599654.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            54980..55345
                     /gene="rpsM"
                     /locus_tag="VW144_000144"
     CDS             54980..55345
                     /gene="rpsM"
                     /locus_tag="VW144_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU9599655.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            55363..55746
                     /gene="rpsK"
                     /locus_tag="VW144_000145"
     CDS             55363..55746
                     /gene="rpsK"
                     /locus_tag="VW144_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU9599656.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            55789..56724
                     /locus_tag="VW144_000146"
     CDS             55789..56724
                     /locus_tag="VW144_000146"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU9599657.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            56736..57122
                     /gene="rplQ"
                     /locus_tag="VW144_000147"
     CDS             56736..57122
                     /gene="rplQ"
                     /locus_tag="VW144_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU9599658.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            57387..57653
                     /locus_tag="VW144_000148"
     CDS             57387..57653
                     /locus_tag="VW144_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU9599659.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAIVSSDEKQDFTYLRNEFEAFGQALNVKINIQSAAIFEAMYNI"
     gene            57663..59000
                     /locus_tag="VW144_000149"
     CDS             57663..59000
                     /locus_tag="VW144_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEU9599660.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIDAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            59244..59936
                     /locus_tag="VW144_000150"
     CDS             59244..59936
                     /locus_tag="VW144_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9599661.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(60039..61684)
                     /locus_tag="VW144_000151"
                     /pseudo
     CDS             complement(60039..61684)
                     /locus_tag="VW144_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(61674..62702)
                     /locus_tag="VW144_000152"
     CDS             complement(61674..62702)
                     /locus_tag="VW144_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9599662.1"
                     /translation="MNITIPKGSLFTLLGPSGCGKTTLLRMIAGFNSIKDGEFYFDDT
                     KINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLMQKKVPKEELIQQTNKYLELMQI
                     AQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEPLSNLEAKLRLDMRQAIREIQH
                     EVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRPKELYHKPANEFVATFIGRTNI
                     IPANLEKRSDGAYIVFSDGYALRMPALDQVEEQAIHVSIRPEEFIKDESGDIEGTISD
                     SVYLGLNTEYFIETGFASKIQVSEESTFEEDLQKGNRIRLRINTQKLNIFSADGSQNL
                     IKGVNHGT"
     gene            complement(62777..63793)
                     /locus_tag="VW144_000153"
     CDS             complement(62777..63793)
                     /locus_tag="VW144_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9599663.1"
                     /translation="MKKKWMYYAACSSNESADDSSSDKGDGGSLVVYSPNSEGLIGAT
                     IPAFEEKYGIKVELIQAGTGELFKKLESEKEVPVADVIFGGSYTQYTTHGELFENYTS
                     KENDNVIKEYQNTTGYSTPYTLDGSVLIVNPDLTKGMNIEGYNDLFKPELKGKIATAD
                     PANSSSAFAQLTNMLQAQGGYKDDKAWSYVKDLFTLIDGKIGLSSSGVYKAVADGEMT
                     VGLSYEDPAVKLLNDGANIKVVYPKEGTVFLPASAAIVKKSKNMENAKKFIDFIISQE
                     VQDTLGTTTTNRPVRKNAKTSENMKPIDKIKTLTEDYDYVIKNKSDIVKKYNEVFTDI
                     QSKQ"
     gene            complement(64010..64207)
                     /locus_tag="VW144_000154"
     CDS             complement(64010..64207)
                     /locus_tag="VW144_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9599664.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSPLSAISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            complement(64282..65058)
                     /locus_tag="VW144_000155"
     CDS             complement(64282..65058)
                     /locus_tag="VW144_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEU9599665.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF"
     gene            65180..65926
                     /locus_tag="VW144_000156"
     CDS             65180..65926
                     /locus_tag="VW144_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9599666.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     RQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            65939..66919
                     /locus_tag="VW144_000157"
     CDS             65939..66919
                     /locus_tag="VW144_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEU9599667.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAVGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
BASE COUNT        20194 a        12432 c        14722 g        19659 t
ORIGIN      
        1 actttcccaa tcgatttgtt ctcatcttat ttcaatctgc tatataatag tccatgctgt
       61 ggctggattc ttttcagcct acttatactc ttcgaaactc tcttcaaacc acgtcagctt
      121 caccttgccg taggtatggt tactgacttc gtcagttcta tccacaacct caaaacagtg
      181 ttttgagctg acttcatcag ttctatccac aacctcaaaa cagtgttttg agcaacctga
      241 ggctagtttc ctagtttgct ctttgatttt cattgagtat tattaagaca atatgaggag
      301 aaagatgaaa gaaaaaggat tttaggaggg ggcgcaggca gccatgccaa cggctcttgg
      361 ctatgtcagt atcggcctgg cctgtgggac tatcggtgcg ccctatgtga cacctgttga
      421 gatgggcttg atgagtctct ttgtttatgc tgggagtgcc cagtttgcca tgttggcact
      481 gattgtggtt caagctcctg tggcagctat tgctatgacg gtttttctaa tcaacttgcg
      541 tctctttttg ttgagtttac acgcatcgac ttatttccgt cataccagtc tttggtacaa
      601 tatcggtatg tctagtatct tgacagatga gacctatggc gttttgatgg gtgaattggc
      661 ccatacagac aaggtaaatc ctatgtggat gcacggaaac aatcttaaca gctatgtggc
      721 ttggtttgtg gggacagtag tcggaacggc tctgggtggc ctgctaccaa atccagaaat
      781 ctttggcttg gattttgccc tggttgggat gtttattggt atttttgctt cgcaatttca
      841 gattatgcaa agacggattc ctgtccgcaa tctgctcatt atcctagcag ttgttgcggt
      901 gtccttcttt ttgctcttga cagtggtgtc tcagtcacta gctgttctgt ttgcgacgct
      961 acttggttgt agcatggggg tggttttaga tggtcagtaa gtatctttta ttagcagtta
     1021 ttttctctgg cttggtgact tggattctcc gtatgattcc cttcatctta gtcaagtata
     1081 agggcttgcc tgcaatcgtt gagcgttttt tgaagttctt gcccgtttcc attatctttg
     1141 ccttgattct ttcaagcgta gtgacaggta aggttgggag ccttcctcaa attaaatggc
     1201 tagacttctt agccgtcttt ccaacagctt gggtagcctt tcgctaccgc aatctagtcg
     1261 gaacagttct ctttggagtg gtcttgattg ccatcttgcg tttggtcttt taaattaccc
     1321 ataaaaaaac ttatcacaga gatagatatc atataatggc gtaaatgctc tttttctgtt
     1381 aagattataa ggtattctat tttggaggaa atgacatgaa aaaaatcgtt aaatactcat
     1441 ctcttgctgc cctaggactt gttgctgcag gtgtgcttgc ggcttgctca gggggtgcta
     1501 agaaagaagg agaagcagct agcaagaaag aaatcatcgt tgcaaccaat ggatcaccaa
     1561 ggccatttat ctatgaagaa aatggcgaat tgactggtta cgagattgaa gtcgttcgcg
     1621 ctatttttaa agattctgac aaatatgatg tcaagtttga aaagacagaa tggtcaggtg
     1681 tctttgctgg tcttgacgct gaccgttaca atatggctgt caacaatctt agctacacta
     1741 aagaacgtgc ggagaaatac ctctatgccg caccaattgc ccaaaatcct aatgtccttg
     1801 tcgtgaagaa agatgactct agtatcaagt ctctcgatga tatcggtgga aaatcgacgg
     1861 aagtcgttca agccactaca tcagctaagc agttagaagc atacaacgct gaacacacgg
     1921 acaacccaac tatccttaac tatactaagg cagatttcca acaaatcatg gtacgtttga
     1981 gcgatggaca atttgactat aagatttttg ataaaatcgg tgttgaaaca gttatcaaga
     2041 accaaggttt ggacaacttg aaagttatcg aacttccaag cgaccaacaa ccgtacgttt
     2101 acccacttct tgctcaaggt caagatgagt tgaaatcatt tgtagacaaa cgcattaaag
     2161 aactctacaa agacggaacg cttgaaaaat tgtctaaaca attcttcgga gatacttatc
     2221 taccagcaga ggctgatatt aaataatttc ttgaaatcat ccattctgag taagatgggt
     2281 gatttctcag tttttaggga tatagtttta aactatatat ctgattatct aataacaatt
     2341 tgtgaaatca aacaaattgt gagatactag tacggtatta tttttaagga gaaagaatca
     2401 tgaaaatcaa aaaatggctt ggcctagtag cccttgctac agtcgcaggt ttggctcttg
     2461 cagcttgcgg aaactcagaa aagaaagcag acaatgcaac aactatcaaa atcgcaactg
     2521 ttaaccgtag cggttctgaa gaaaaacgtt gggacaaaat ccaagaattg gttaaaaaag
     2581 acggaattac cttggaattt acagagttca cagactactc acaaccaaac aaagcaactg
     2641 ctgatggcga agtagatttg aacgctttcc aacactataa cttcttgaac aactggaaca
     2701 aagaaaacgg aaaagacctt gtagcgattg cagatactta catctctcca atccgccttt
     2761 actcaggttt gaatggaagt gccaacaagt acactaaagt agaagacatc ccagcaaacg
     2821 gagaaatcgc tgtaccgaat gacgctacaa acgaaagccg tgcgctttac ttgcttcaat
     2881 cagctggctt gattaaattg gatgtttctg gaactgctct tgcaacagtt gccaacatca
     2941 aagaaaatcc aaagaacttg aaaatcactg aattggacgc tagccaaaca gctcgttcat
     3001 tgtcatcagt tgacgctgcc gttgtaaaca ataccttcgt tacagaagca aaattggact
     3061 acaagaaagc acttttcaaa gaacaagctg atgaaaactc aaaacaatgg tacaacatca
     3121 ttgttgcgaa aaaagattgg gaaacatcac ctaaggctga tgctatcaag aaagtaatcg
     3181 cagcttacca cacagatgac gtgaaaaaag ttatcgaaga atcatcagac ggtttggatc
     3241 aaccagtttg gtaataagaa acagggaggt gggagagaga attccacctc ttgcttttgt
     3301 atagagcacg gagtgtaaag aagactattc gttcatagaa aggtagagag aatatggttt
     3361 ttcctagcga acaagaacag attgaaaaat ttgaaaagga tcatgtagcc cagcattatt
     3421 ttgaggtttt gcgtaccttg atttctaaga aatcagtctt tgcccagcag gttggactca
     3481 aggaagtcgc aaattatctg ggtgagattt tcaagcgtgt tggagctgaa gtggagattg
     3541 atgagagcta tacagcgccc tttgtcatgg cacatttcaa gagttcgcgt ccagatgcca
     3601 agaccttgat tttctataac cactatgaca ctgtgccagc ggatggggat caggtctgga
     3661 cagaggatcc ttttacgctt tcggtccgca atggcttcat gtatgggcgt ggagttgatg
     3721 acgacaaggg tcatatcaca gctcgcttga gtgctttgag aaaatatatg cagcaccatg
     3781 atgatttacc tgtcaatatc agctttatca tggagggagc ggaggaatcg gcttcaacag
     3841 acctagataa gtatttggaa aagcatgcag acaaactccg tggggcggat ttgttggtct
     3901 gggaacaagg gaccaaaaat gccttggaac agctggaaat ttctggtggc aataagggga
     3961 ttgtgacctt tgatgccaag gtaaaaagcg ctgatgtgga tatccactcg agttatggtg
     4021 gtgttgtgga atcagctcct tggtatctcc tccaagcctt acagtctctt cgtgctgcgg
     4081 atggccgtat cttggttgaa ggcttgtacg aagaagtaca agagcccaat gaacgagaaa
     4141 tggccttgct agaaacttat ggtcaacgaa acccagagga agttagtcgg atttatggat
     4201 tggagttgcc tctcttacag gaggagcgga tggcctttct aaaacgtttc tttttcgagc
     4261 cagcgcttaa tatcgaagga atccagtctg gttatcaagg tcagggtgtt aagactattt
     4321 tacctgcaga agccagtgcc aagctagagg ttcgtctggt tccgggccta gaaccgcatg
     4381 atgttctgga aaaaattcgg aaacagctag acaaaaatgg ctttgataag gtagaattat
     4441 actatacctt gggagagatg agctatcgaa gcgatatgag cgcaccagcc attctcaatg
     4501 tgatcgagtt ggccaagaaa ttctatccac agggcgtttc agtcttgccg acgacagcgg
     4561 ggacaggacc tatgcatacg gtctttgatg ccctagaggt accaatggtt gcattcggtc
     4621 taggaaatgc caatagccga gaccacggtg gagatgaaaa tgtgcgaatc gctgattatt
     4681 acacccatat cgaattagta gaggagctga ttagaagcta tgagtagaga tattatcaag
     4741 ttagatcaga tcgatgtgac ttttcaccaa aagaagagaa ccatcacagc ggttaaggat
     4801 gtgaccattc acatccaaga aggggatatc tacggaatcg ttggatattc tggagcaggg
     4861 aaatcaaccc ttgtacgggt gattaatctc ttgcaaaaac catctgcagg gaaaattacc
     4921 attgacgacg atgtgatttt tgatggtaag gtcaccttga cggcagagca gttgcgtcgt
     4981 aaacgtcaag atatcgggat gattttccag cattttaacc tgatgagtca aaagacagca
     5041 gaggagaatg tagcctttgc ccttaaacac tctggactca gcaaggaaga aaagaaggct
     5101 aaagtagcta agttgttgga cttggttggt ttggcagatc gtgctgaaaa ctacccttca
     5161 caattatctg gagggcaaaa acagcgtgtg gcaattgcgc gtgccttggc caatgatcca
     5221 aaaatcttga tttcagacga gtcaacttct gcccttgacc ctaagacaac caagcagatt
     5281 ttggccttgt tgcaagattt gaaccaaaaa ttaggcttga ctgttgtctt gattacgcat
     5341 gaaatgcaga ttgtcaaaga cattgccaac cgtgttgcag ttatgcagga tgggcatttg
     5401 attgaagagg gtagtgtgct tgaaatcttc tcaaacccta aacaaccttt gactcaagac
     5461 tttatctcaa cagctacagg tattgacgaa gccatggtca aaatcgagaa gcaagaaatc
     5521 gtggaacact tgtctgaaaa cagtctcttg gtgcaactca agtacgctgg agcttcaaca
     5581 gacgagccac ttttgaatga attgtacaag cattaccaag taatggctaa tattctctat
     5641 gggaatatcg aaattctcga tggtactcct gttggagaat tggtggtggt tttgtcaggt
     5701 gaaaaagcag cgttggcagg tgcccaagaa gccattcgtc aagcaggtgt acaactaaaa
     5761 gtattgaagg gagtacagta agatggaatc attgattcaa acctatttac caaatgtcta
     5821 taaaatgggt tgggctggtc aggcaggctg gggaacggct atctacttaa ctctttatat
     5881 gacagttctt tccttcatta tcggaggctt cttggggcta gtggcaggtc tctttctcgt
     5941 cttgacagcg ccaggtggtg tcttggagaa taaagtcgta ttctggattt tagacaaaat
     6001 tacctcaatt tttcgtgcgg ttccctttat catcctcttg gcaatcttgt caccactttc
     6061 tcacttgatt gttaaaacaa gtatcgggcc aaatgcagcc cttgtcccac tttcttttgc
     6121 agtctttgcc ttctttgccc gtcaggtgca ggttgtcttg gctgaattgg atggcggtgt
     6181 cattgaggcg gctcaagcga gcggagcgac tttctgggac atcgtgggtg tttacctatc
     6241 agaaggtctt ccagatttga tccgtgtgac gactgtgacc ttgatttccc ttgttgggga
     6301 aacagctatg gccggtgcgg ttggagctgg tggtatcggt aacgtagcca tcgcttatgg
     6361 atttaaccgc tacaatcacg atgtgaccat cttggcaacc atcattatca ttttgattat
     6421 ctttgcaatc caattcttgg gagatttctt gactaagaaa ttgagccata aataaaaaag
     6481 agccgcgtgg ctctttttaa ctgatcagat tttctgtgca aattttttac tcaaggcttg
     6541 tccaatcaag gcacccacta gggctccgat gacaatactt gcgataaata gaaggacagt
     6601 tccagggttt ggagcgacca tgatgcggtc gatatattct tgggattttc ctcttgccag
     6661 aagagtagcc atataggctt tgggcgcaat ccacataagc aagattggtc ctgttgtact
     6721 aaaggcgaaa ataatgaaag aaaggaagtt ctttgttttg tccttgtatt ttcctaaatg
     6781 agctacttca tctgctagga ggccacagat aattccagga aggaaggaac cagcaccgtg
     6841 tttagttccc aagaaaaaga gggcaatgac aaggccgata gtggtaatgg ctccaaagcg
     6901 cggaactttt gcgactagga tcatatagac gctaccgccg acaagggcag taaaggcagg
     6961 cgcataaaac atgtttccag tttggtcaaa gagattgccc aaaaggacac caatccccat
     7021 gcagaggaag taaagaactg caaaaagcag tgtagttaag atagatttct tcatgaattg
     7081 tctcctgata attttttcac aattctcatt gtaccacggt ttggtgggat tgtaaatgga
     7141 tactagaagt ttttgaaaac gcttgaaata tgatataatg gtttcatagt tatttttagg
     7201 ggaatatcat gggtagattt ttagactttg tctttaatcg tttcttttta gggatgattg
     7261 cgacagcctt cttttggcta ttaactttag taggagggat tatccttggt ctagcgccag
     7321 ctagtgccac cttgatgagc ttatatgcag aacatggtta tagctttcgg gaatacagtt
     7381 tgaaggaggc ttggtctctt tacaagcaaa attttgtctc aagcaacctg attttctata
     7441 gctttttagg tgtgggtcta gttttgacct atggtttgta tctcttggtg caattgcctc
     7501 atcagaccat tgttcatttg attgcgaccc ttttgaatgt cctagtagtt gccctgatct
     7561 ttttggctta tacagtatct ttaaaattac aagtttattt tgccttgtcc tatcgaaata
     7621 gtctcaaatt atccttgatt ggcatcttta tgagtctagc agctgtggcc aagattctcc
     7681 ttgggactgt gctacttgta gcaattggtt actatatgcc tgccctgcta ttttttgtag
     7741 gaattgggat gtggcatttc tttatcagtg atatgttgga acctgtctat gaaatcatcc
     7801 atgaaaaatt ggcgacaaaa tagaatgaag cacttttggc tacatacgct tctaagaacc
     7861 tatagttcag tgatgatcat tatcattgcg agttttgcaa tcttactctc ttacgctgac
     7921 tgggattcac gtgaaaagga agcccagaga gtagcccagc gtgtaactgc tagaacagtg
     7981 agtgaaattg aatattacca tagagagtca acccagatag ctcaggcttt agttgaaaac
     8041 caagctcgta ttgagggaat ctataaatac tttagcctta gcatgccaga ctatttttac
     8101 tggcaattag agcggaaagc ttcgccttat atatcagtct ctctgtatga aaatgttgat
     8161 gatctctatg ttcgaaatga ttttgtaact ggggtggcca ttgcttttca agattacaag
     8221 gaagtctatg tttctactaa agacaaacgt agtggagaaa aaatcagggc tgaggatttc
     8281 aaaccagcag gaaatagttt tgccattcca gtgtcagatc cagtgtcaga tcaagactta
     8341 ggagtgattt acatctcctt ggatcctgct gttttatacc atgccattga taatactaga
     8401 ggtcatactc cgatggcagt aacagtgacc tcaccttttg atacggagat ttttcatatg
     8461 ggtgagacag ttgataagga gagtgaaaat tggctagttg gcttaacttc tcatggttat
     8521 caggttcagg tggcagttcc caaaaacttt gttttacaag gaacggtgac cagctctgct
     8581 ttgattgtgg gcttgagcct tctctttatt gtcattcttt atctgacttt gaggcagacc
     8641 tttgctaatt atcaaaagca ggtagtggat ttggtggatt ccatccaagc tattgcccaa
     8701 ggacaagaag gtcttcgcat tgatacgctt gaaaaggatc aggaattgct cctaatcgcg
     8761 gagacgacca atgatatgtt ggatcgattg gaaaagaata tccatgatat ttaccagtta
     8821 gaactcagtc aaaaagatgc caatatgcgg gccttgcagg cgcaaatcaa ccctcacttc
     8881 atgtataata cgctggagtt cttgcgtatg tacgcagtca tgcagagtca agatgagttg
     8941 gcagatatca tttatgaatt cagtagtctc ttgcgtaaca atatttccga cgaaagagag
     9001 accctcctca aacaggaatt agaattttgc cgtaaataca gctatctctg catggttcgc
     9061 tatcccaagt ccattgccta tggtttcaag atagatccag agttagagaa tatgaagatt
     9121 cccaagttta ccttgcaacc gctggtagaa aactatttcg cgcatggtgt tgaccacagg
     9181 cggacagata atgtgattag catcaaggct cttaaacagg atgggtttgt ggaaattttg
     9241 gtggtcgata atggtagagg aatgtcggct gaaaagttgg caaatatccg agaaaaatta
     9301 agtcagaaat attttgaaca ccaagccagc tacagtgatc aaaggcagtc tatcgggatt
     9361 gtcaatgtac acgagcgttt tgtgctctat tttggagacc gctatgccat tactatagag
     9421 tctgcagagc aagccggtgt tcagtatcgt attacaattc aagatgagta gaaagggaga
     9481 aaatgtataa agtattatta gtagatgatg agtacatggt gacagaaggt ctgaagcgtt
     9541 tgattccctt tgataagtgg gatatggagg tcgtcgcaac agccagtcat gccgatgaag
     9601 ctctagaata tgttcaggaa aatcctgtcg atgtcatcat ttccgatgtc aatatgccag
     9661 acaaaacagg gcttgatatg attcgggaga tgaaagagat cttaccagat gctgcctata
     9721 tcctgctctc aggttatcag gagtttgatt atgtaaaaag agcaatgaac cttagtgtgg
     9781 tggactattt ggtcaagcct gttgataagg tagagctggg aaatctgctg gagaagattg
     9841 caggtcagct cggcgagaga gggaagaaaa gtcagactct tagtcaagaa ttagacgagg
     9901 ctggatttgt tagttattta ggggataagg agaattggtg gataggtcta tccaaggaaa
     9961 aacaaggttc cttcaccatt ccctactatg tcttgggcca agactggcag attttcattt
    10021 ctgaccaacc cctagatggt ttagtcgtta caccttttga agctccttat caagaacact
    10081 ttgaacgctg gaagctgaat gctgagaaaa ccctctttta cggttctgta aatttgcagc
    10141 agtctgagag tctctttgcc tattacgaac cgatttatag ggttatcatt cagggaaatc
    10201 tcaatcaaat cgtagaagag ttaaatctct tggagaaggt agttcttgaa aatacgccgc
    10261 gtgttccgat tactaaacag ctttttattc agtttgtcat ggatgttttc catttatttg
    10321 aacatctcaa agctgatgac ctgacagaca tcgtcaaaac cattcatgct attcaatcct
    10381 tcgatgaatt ggttccttat atcaaggaaa ctctgagcag ctttttcggt caataccgta
    10441 tgaatgaaaa tgtggtcagt gtgctggaag tcattggtcg tgattaccaa aaagagcttt
    10501 ccctcaagga tatcagtaag gctctcttta tcaatcctgt ctatctaggg cagttgatta
    10561 agcgtgaaac cgattcgact tttgcagagt tactaaacaa acaacgtatt aaggctgccc
    10621 aacaactttt gctttcaact agtgacagca tcgaagatat ttgttatgct gttggttaca
    10681 gtaaccttgg atatttctat aaagttttcc gaaaattgtg cggaaaatcg ccaaaagcct
    10741 accgaaaaca ggtagaaact atactataag atttgtattc ctttacaaaa ggtgctataa
    10801 tattatcagg aggtcctatc atgaaaaaga aaccaattta tctatgggtc ttgctaatct
    10861 tgtctgccct tatttcagtt ccgtctctgt ttggaattgt gagtcccttg cctagtaaag
    10921 aagcccttcg tgctgctcag aaacaagttg caggggtcaa tgctcagcaa ttagaagacc
    10981 agcttaatta tacctataga gtagcagaag catctcattc tatttttaat gttgctttga
    11041 ttgtgctatc tactatttta gtggtcgtag cgattgtctt ccttgttcgt aaaaatttgc
    11101 aatatgcaaa ctatacttac gttggctatg ttttgttagc tattattggt tcgatttatg
    11161 gctatgttgg tttacaagac gctgtgcagt tggttcaaga tgagagcatg cgtttgacag
    11221 tgagtattgg ctcaaaagct gttagcattt tctatatcgt catcaatgtt ctcttcctag
    11281 cccttgtctt ctataagatg tggcgacaac agaaagcctt ggcagaagaa gaggaaacag
    11341 aagagcttac ctaagtattg acaaaaaaga actatcaaga tacaatcttg gtagttgttt
    11401 tttgattata gataaaatca tggaggtaca tatgaagaca atttctttag tttatattag
    11461 tttgagtggc aacactgaaa gttttgtgac gcgcttgaaa gactatctct tgtcccagta
    11521 caaagggatt gaggttcaaa agattcatat caaggatttg gtcaaggaag gcaaaaattt
    11581 ctatgaaatg gaccatccct atgtcgcttt tttgccgacc tatctcgaag gtgggaatgg
    11641 cgtggataac ggagatgttg agattttgac gacaccggtg ggagatttta ttgcctatgg
    11701 taacaatgct agtaagtgtt ttggtgtggt tgggtcagga aatcgtaact ttaataacca
    11761 atactgccta acagccaagc aatacagtca acgctttggc ttccctgtat tggctgactt
    11821 tgaaatgcga ggcatgctgg aagatatcaa acatgtcgca gctattatcg cagatttgta
    11881 tgagttggaa aaggagaatt aatactcaat gaaaatcaaa gagcaaacta ggaagctagt
    11941 cgcaggttgc tcaaagcaca gctttgaggt tgcagataga gctgacgtgg tttgaagaga
    12001 ttctcgaata gtataaagtt cttataagag gtggttgaag ttgttcaact gttttttgag
    12061 tataaacagt ctttaagaac gtaaaaaagc ataagttcag gtcttcaaaa ccttgttctt
    12121 atgcttttta tcatagaaat atttgctgaa ttttctcttg aaatgaaacc ttatcttcat
    12181 ctcgcgcttg tcccttaccc tgcttttccg attgcgagtg cgtagataaa gaagatggcg
    12241 aagccaaaga ggaaaatcaa tcccgcaatg gcaaggtgtt tgagccaaga aggaagattt
    12301 ttgttttcac gcgttaccaa ggcgtaattc aaaagagcaa agaaaggtgt tgtcaggaaa
    12361 gatccaatca tggcaaagcg gagcatggtt gaaacctgac cagcgaagaa cttgataatg
    12421 acgataccga tgatagcagt gatggtcatc cagatgttca aagatttacg attgtcctct
    12481 ttttgactga ttagcagtcg gagagattcc tgattaacgc gagaatagcc atcgataaca
    12541 gttataactg ttccaaagat acagaggaag gcaataaagg taatcaagta acgggaccat
    12601 tcgccaagaa cagaggcata catgcccacg aattgagaga tgtatttggc tgaagcagct
    12661 tcaaccgcct gccctgtagg atactgaatc agtgctccca gtgccacaaa gaagacggct
    12721 aggatagctg ttccaatata accagtgtta aagtcaaaca gagcgtcttc tgtgttaaag
    12781 ttgacggtct ttctcttttc agctgaccaa agtgaattga tggctgaaat ttcaatagga
    12841 gccggcatcc atcctaggag ggagacgatg aagggcagag ctgccatttg ccaaggtgtc
    12901 ttctcgacaa aatcagaact gtattctgga tgcttgaccg ccgcaatgat aactgcaaga
    12961 acagtcgcaa tggttaaggc agacataatc catttgacca tgccgtctaa aagtttgtag
    13021 cctccaaaga gtagcatagc ccaaatgatt gcaacgagaa tgagggacca ctgagtaatg
    13081 ctaagtccaa tcattgggaa ggcactggcg atgatagctg agcacagaat ggcaacacca
    13141 gccgtgttga ccatagccga aaagacattg agaataaaga aaatccagag atagagtttt
    13201 cctttttcgg cataaccttc aaccaaagtc tttccagtat cagctgtgta ttcagcacca
    13261 aaacggaaaa atggatattt aaagacattg gctaagatga ccaagagaag tagagaccaa
    13321 ccgtaagaac cgccagcttg agttgaggat acaatgtggg aacctccaac agcggcagtt
    13381 gccattagga ttccaggtcc cattgctttt agcttgcttg cccaggttga ttgatttaag
    13441 gaaatagctt gcgacatgat aaatactcct ttttgaattt tcagaataat ctgtatatgc
    13501 aatctatatt ctacaaaaaa ttttggaaaa atgcaagaat attttgaaaa aagatagaaa
    13561 atttacaaag aaaatctgaa ataactgtta tgatatagca gattgaaact agaatagtac
    13621 acttttgctt ctaaaacatt gttagaaatc gatttgactg tcctgattga tttatcctgt
    13681 tcttatttca tttcactata gtaaggtgca gcaggataaa gcaaaaccga gaagtttctt
    13741 tctcggtttt gcttattatg aggttcctat tttctatgct ccaagagttg attgatgtga
    13801 tctacttttt tagcctctct tttatagaga atagcccaga tgatgagata gacaatcgca
    13861 aactcgataa tgagctggag gtagaaaatc cagtggaagg ggaaccaacc agcaagagtt
    13921 gctagtggga caaagccagc cagcataagg aagaaatgag tcagagtggc acgaagcatg
    13981 ctccagtcac ggctgaataa gcggttgcca aagttgaaga gcataccgat ggctgcccag
    14041 ataagtgtgc agtagagcaa gaccagggca ccgtgaacct gatgctgatc catcacttgg
    14101 ccgatgagag agtagggatt tagtggtgcg taggtatttg gtgcataaat gagtgaaaag
    14161 aggatggaga ggatgaggcc gataagaaca ccggcagctg catcgtgaaa tacttgtttt
    14221 ttcatagttc taatttctcc ttgatggttt ttagataacg gcgtgaagag taggtgaagc
    14281 tttcgttttt caagaaaatt tctaccagac cgtttggcgt gagcttgagg tgagagatgg
    14341 aatcgatatt gatgatttct gattgggaaa tttggataaa attggttggc aagagtttaa
    14401 gaacctgata gagtcgcaaa tcaatgctgt aggtctgact cgcggtttct gctagaacct
    14461 tccgattctc gatatagaag cgctgaatct tgccaatctc aactagatag acctgatcat
    14521 cgatttttcc tttgattttt tctctttggt ccagattttc tgcgaactcg atgactttct
    14581 ggactttttc ggtttcttga ggtgcttgga caatcagctt ttcctcctcg taagtctcac
    14641 taatctgtag ttctactttc ataaatttct ctccttgttt ttcatacaaa attaatctca
    14701 cttcttgtac acctttatta atactcttca aaaatctctt caaaccacgt cagcgtcgtc
    14761 ttgccgtagg atagttgttg actttgtcag ttttatctac aacctcaaaa cagtgttttg
    14821 agctgtctgc gtctagcttt ctagtttgct ctttgatttt tattgagtat accactattt
    14881 tactccctct ggcaagggac tttgtctatg tggagggatt gggctcctat gtggtggagt
    14941 ttttctgttc tttctgaaat atggtataat agcactaatt aatttctagg aaaatagata
    15001 cagaaagggg ctgaaagatg tctcatatta ttgaattgcc agagatgctg gcaaaccaaa
    15061 tcgcagctgg ggaggtcatt gaacgtcctg ccagtgtggt taaagagttg gtagaaaatg
    15121 ccattgacgc gggctctagt cagattatca ttgagattga ggaagctggt ctcaagaagg
    15181 ttcaaatcac ggataacggt catggaattg cccacgatga ggtggagttg gccctgcgtc
    15241 gccatgcgac cagtaagata aaaaatcaag cagatctctt tcggattcgg acgcttggtt
    15301 ttcgtggtga agccttgcct tctattgcgt ctgttagtgt cttgactctg ttaacggcgg
    15361 tggatggtgc tagtcatgga accaagttag tcgcgcgtgg gggtgaagtt gaggaagtca
    15421 tcccagcgac tagtcctgtg ggaaccaagg tttgtgtgga ggatctcttt ttcaacacgc
    15481 ctgcccgtct caagtatatg aagagccagc aagcggagtt gtctcatatc attgatattg
    15541 tcaaccgtct gggcttggcc catcctgaga tttcttttag cttgattagt gatggcaagg
    15601 aaatgacgcg gacagcaggg actggtcaat tgcgccaagc aatcgcaggg atttacggtt
    15661 tggtcagtgc caagaagatg attgaaattg agaactctga cctagatttc gaaatttcag
    15721 gttttgtgtc tttacctgag ttgactcggg ctaaccgcaa ttatatcagc ctcttcatca
    15781 atggccgtta tatcaagaac ttcctgctca atcgtgctat tttggatggt ttcggaagca
    15841 agcttatggt tggacgtttt ccactggctg tcattcacat ccatatcgac ccttatctag
    15901 cggatgtcaa tgtgcatcca actaagcaag aggtgcggat ttccaaggaa aaagaactga
    15961 tgaccctggt ttcagaagct attgcaaata gtctcaagga acaaaccttg attccagatg
    16021 ccttggaaaa tcttgccaaa tcgaccgtgc gcaatcgtga gaaggtggag caaactattc
    16081 tcccactcaa agaaaatacg ctctactatg agaaaactga gccgtcaaga cctagtcaaa
    16141 ctgaagtagc tgattatcag gtagaattga ctgatgaagg gcaggatttg accctgtttg
    16201 ccaaggaaac cttggaccga ttgaccaagc cagcaaaact gcattttgca gagagaaagc
    16261 ctgctaacta cgaccagcta gaccatccag agttagatct tgctagcatc gataaggctt
    16321 atgacaaact ggagcgagaa gaagcatcca gcttcccaga gttggagttt ttcggacaaa
    16381 tgcacgggac ttatctcttt gcccaagggc gagatggact ttacatcata gatcagcacg
    16441 ctgctcagga acgggtcaag tacgaggagt accgtgaaag cattggcaat gttgaccaga
    16501 gtcagcagca actcctagtg ccctatatct ttgaatttcc tgcggatgat gccctgcgtc
    16561 tcaaggaaag aatgcctctc ttagaggaag tgggcgtctt tctagcagag tacggagaaa
    16621 atcaatttat tctacgtgaa catcctattt ggatggcaga agaagagatt gaatcaggca
    16681 tctatgagat gtgcgacatg ctccttttga ccaaggaagt ttctatcaag aaataccgag
    16741 cagagctggc tatcatgatg tcttgcaagc gatctatcaa ggccaatcat cgtattgatg
    16801 atcattcagc tagacaactc ctctatcagc tttctcaatg tgacaatccc tataactgtc
    16861 ctcacggacg tcctgttttg gtgcatttta ccaagtcgga tatggaaaag atgttccgac
    16921 gtattcagga aaatcacacc agtctccgtg agttggggaa atattaaaag tataaaaaag
    16981 tctgggaaaa attttcaaaa tcagaaaaac gcataaaatc aggtgttcaa aaaccttgat
    17041 tttatgcgtt ttatcatgga aatagttact tcatgttttc ctaattcttt tcgaaactct
    17101 ttttaaacca cgtcagtttt atcagtaatc tcaaaacagt gttttgagct aattttgcca
    17161 gttttgtctg taacatcgaa gttgtgtttt accactctgc gactggtttc ctagtttgct
    17221 ctatgatttt cacagagtat taaattgcga ttttgccaag tttctttatt cgtctaaaag
    17281 tagagtctgt tctatgcgtc taatgtacga attaggttga ccatttcaat agctccttgt
    17341 gcacactcag aacccttatt tcctgctttg gtaccagctc gttctatggc ttgttcaatt
    17401 gtatctgtcg ttagcacacc aaacataaca ggaatttcgc tatttaaact gatttgggcg
    17461 attcccttag atacctcgct acatacataa tcataatgac ttgtattccc tctaatgaca
    17521 gctcccaagc agataattgc atcatatttt ttactttttg ccatttttga tgcaatcagt
    17581 ggtatttcaa aagctcctgg aacccaggct acctctatat ctttctcgtt tacattctct
    17641 cttttgagat tatctagtgc tccagataat aattttgaag ttataaattc attaaatctc
    17701 gctacaacaa tacctatttt aatattgttt gctactaaat taccttcata agtgttcatt
    17761 tatttttcct ccatatttaa aatgtgaccc attcgatttt tctttgtttc taaataaaaa
    17821 ctatcgtaag gattggcttc tatttcgatt gatattctac tggaaatggt aattccatat
    17881 ttttctaact gttcaacctt gtcaggatta tttgtcagta aatgaagtga ctgaagtccc
    17941 agatctttaa gcatttgtgc tccaatatga tattctctta aatcaccttc aaagcctaat
    18001 gcaagattgg catcaagcgt atccatgcct tgatcttgta aatgataggc ttttaattta
    18061 ttgataagtc caattcctcg tccctcctgt cgcaagtaaa gtaagacacc cgaaccattc
    18121 tcaacaatca ttttcatagc tttatcgaat tgctgtccac aatcgcaacg taaagagcct
    18181 aaaacatctc ctgttaaaca ttcggagtgg acccgacata atacattggc ttcatcctct
    18241 atgtttccca taataagagc aagatgatgt tccccattta gtttatctat atagctaatt
    18301 gctttgaaat taccgtatct agtaggcata ttgacagttg aaactcgttc taccagctga
    18361 tcatatactt ttctatattc ttgtaattct ttgatggtaa ttagtggaat gttgtgtttc
    18421 ttcgaaaact gaattaaatc atctgttctc atcattttgc catcatgatt cattatttca
    18481 caacataggc cacactcttt tagtccagct aattttaata aatcaacagt tgcttctgtg
    18541 tgtccatttc tttctagaac accacctttt tttgcaatta aaggaaacat gtgtcctggc
    18601 ctgcgaaaat cagagggtgt tatatcttca gctacacaca tacgtgcggt cagtcctctt
    18661 tcctcggcag aaatacctgt ggtcgtttct ttataatcaa ttgaaactgt aaaagcagtc
    18721 ttatgattat ctgtattgtt ttcaaccata ggtgaaagca ttaattgatt agctaaactt
    18781 tcgctcatag gcatacaaat taatcctttg gcataagtag ccataaaatt aacattttct
    18841 gttgtagctg cttgtgcaga acaaattaag tctccttcat tttctctatc cttgtcgtct
    18901 ataacaagaa caagtcgtcc cttctgcaat gcttctaatg cttcttgtat ttttcgatat
    18961 tccattgact gattatcctt tctgctaaaa tccattttga tataatagtt tcttagatat
    19021 ttctgatttt ggagagttat ccatcagttt ttgcacatat ttacctaaga tatcattttc
    19081 aagatttact gtactcccga cttgtttact cttaagaatg gtttgttcca aggtatgagg
    19141 gataacagat actgaaaagt ttactttgga gactttagcg acagtcagac taatgccgtc
    19201 aattgtaata gatccttttt caactattaa atctaaaatt tctttttgtg tgttgatttg
    19261 ataccataca gcattatcat ctttttttat tgacgagatt tttcctgtac catcaatgtg
    19321 tcctgtaacg acgtgacccc caagtcgacc gttgacagat aaggctcttt ctagattcac
    19381 ctcacttcca tgttttaata gagtaagagc tgttcgactc catgtttcat tcattacatc
    19441 aactgtaaag gattgatgat tgaaatgagt aactgtaaga cagataccat ttactgctat
    19501 actatcgcct aaatggatat ccgttaatat ttttgaggct ttaattgata gtttacaatt
    19561 acgagagtct ttctgtattc tttcaacttt tccgatttct tcaattattc ctgtgaacat
    19621 ggataaatca cttcactttc tatgagatag tcatttccta tttgagaaaa agcataaggt
    19681 ttcaatctaa tagcgtcatt tggcaaagaa atgccttcac ctccgacagg aaacttggca
    19741 ctgcctccaa aaatttttgg tgcaatatat attttcagct catcaacaat ttgttgttcc
    19801 aaagcactcc aattcattag actgccccct tctagaacta ggctatcaat ctgcatgttt
    19861 cctagatgtt gcattaaact cgataagtct atatgattgc cttttttctt tatggaaagt
    19921 atttcacagc catgattttg atatagcttc attttatttt tgtcttcaga ggaagtggca
    19981 atgtaagttt taatatcatt tgctgttttt acgattttag aggtaagagg agttcgtaaa
    20041 tgtgtatcgc atatgatacg gataggattt ttcccttcct ccaatctaca tgtcagcaaa
    20101 ggatcgtctt gaataacagt attgactccc accataattg cactaacata gtgtcgtaac
    20161 tgatgcacat gctttcttgc ttcttcttca gtaatccatt tggattgatt tgttttagtg
    20221 gctatttttc catccattga cattgcatat ttcataaaaa catagggtac atgctgggta
    20281 atatactttc taaaactttt tattaagtta agacactcat tttctaaaat tccaacagta
    20341 acttgaagat tattttcctc aagtatcttt actccttttc cagatacaat aggattacag
    20401 tctaggcttc caatgactac tcttgtaata ccactatcga ttatagcatc tatacaggga
    20461 ggtgttttcc cgaagtgaca acagggttca agtgttacat aaagcgtcgc tccgacaggg
    20521 gattctctac agtttttaag agcatttctc tcagcatgtg ggccaccaaa aaactcatga
    20581 taaccttgtc cgatgatgtg attatctttt acaataactg cgccgaccat aggattggga
    20641 ttgacgtaac cagccccttt ttgtgccagt tttattgcta atttcatata ttttgaatcg
    20701 ctcatctcac tacctccaaa aaaaatatac cttgaatagg ggactactca aggcatacaa
    20761 aagaaaactt atgcgattaa caaaaatact ctgaaatgac aagtaatcat ttcagagcac
    20821 gcaaaaagca caaatatact tttatcttct ttcatccaga ctatactgtc ggctttggaa
    20881 tttcaccaaa tcatgccttt cggctcgtgg gctataccac cggtagggaa ttacaccctg
    20941 ccctgaagat agttattcaa ttacagatga ttatagtact taattttgaa tatgtcaaca
    21001 gataaatacc gattgttttt gatatactgt atttgtgata atcgattctc gctcctcgga
    21061 taaagaaaat atgatatact agataaacga aataagagag aaggaatact atgtacgcat
    21121 atttaaaagg aatcattacc aaaattactg ccaaatacat tgttcttgaa accaatggta
    21181 ttggttatat cctgcatgtg gccaatcctt atgcctattc aggtcaggtt aatcaggagg
    21241 ctcagattta tgtgcatcag gttgtgcgtg aggacgccca tttgctttat ggatttcgct
    21301 cagaggatga gaaaaagctc tttcttagtc tgatttcggt ctctgggatt ggtcctgtat
    21361 cagctcttgc tattatcgct gctgatgaca atgctggctt ggttcaagcc attgaaacca
    21421 agaacatcac ctacttgacc aagttcccta aaattggcaa gaaaacagcc cagcagatgg
    21481 tgctggactt ggaaggcaag gtagtagttg caggagatgg ccttcctgcc aaggtcgcag
    21541 tgcaagcaag tgctgaaaac caagaattgg aagaagctat ggaagccatg ttggctctgg
    21601 gctacaaggc aacagagctc aagaaaatca agaaattctt tgaaggaacg acagatacag
    21661 ctgagaacta tatcaagtcg gcccttaaaa tgttggtcaa ataggagcag agaatgacaa
    21721 aacgttgttc gtgggtcaag atgaccaacc cgctctacat cgcctatcat gatgaggagt
    21781 ggggtcagcc cctccatgat gaccaagcat tgtttgagtt gttgtgtatg gaaacctatc
    21841 aggcaggcct gtcttgggaa acggtactca acaaacgcca agctttccga gaagcctttc
    21901 atagctatca aattcactca gtcgcagaga tgactgagac tgaattggaa gccatgctgg
    21961 agaatccagc tatcattcga aatagagcca agctttttgc tacacgcgct aacgcccaag
    22021 cctttctaca gttacaggca gagtacggct cttttgatgc ctatctttgg tcttttgttg
    22081 aggggaaaac tgtcgttaac gatgttcctg attatcgcca agccccagct aaaacaccct
    22141 tatctgagaa attagccaaa gatctcaaaa aacgaggctt caagttcaca ggcccagtcg
    22201 ccgtattgtc ttttctacag gctgcagggc tagttgatga ccacgagaat gattgtgagt
    22261 ggaaaggtct taaatgatgt ctaacaaaaa taaggaaatt ctgatttttg cgattctcta
    22321 tacagtcctc tttatgtttg atggcgttaa attgctggct tctttaatgc catctgccat
    22381 tgcaaattat cttgtttatg tagttttagc tctatatggc tccttcttgt tcaaggatag
    22441 attgatccaa caatggaagg agattagaaa gactaaaaga aaattcttct ttggcgtctt
    22501 aacaggatgg ctctttctca ttctgatgac tgttgtcttt gaatttgtat cagagatgtt
    22561 gaagcagttt gtgggactag atggacaagg tctaaatcag tctaatattc aaagtacctt
    22621 tcaagaacaa ccactactga tagctgtttt tgcttgtgtc attggacctc tggtagaaga
    22681 attatttttc cgtcaggtct tattgcatta cttgcaggaa cggttgccag gtttactaag
    22741 cattattctg gtaggacttg tttttgcttt gactcatatg cacagtttgg ctctatcaga
    22801 gtggattggt gcagttggtt acttaggtgg aggccttgcc ttttctatta tttatgtgaa
    22861 agaaaaagag aatatctact atcccctact tgttcacatg ttaagcaaca gcctctcctt
    22921 aatcatttta gctatcagta tagtaaaatg aaataagaac aggacaaatc gatcaggaca
    22981 gtcaaatcga tttctaacaa tgttttagaa gcagaggtgt actattctat tttcaattta
    23041 ctgtaatatg tgatgaaaat gccagtaatg ataccgagaa aaaagctgag aaacttttcc
    23101 cagctttatt tgttatagtc aaagcgaatg acttgttcct gtgcatctac atgagcatgg
    23161 accccaaagg gtacaattgc tcttggagtt gcgtggccga cattcagatt atagacaatc
    23221 gggatattgc tgtcaatgat atccaatagt gcctctttat agtcgtcatg gaaagtttca
    23281 tccataggtt ttccgaccaa gagtccattg atgaccgcga atatgccagt gtcctttaaa
    23341 gttagcaaca tctttttgaa gtcttctggc ttaggctttt cttcgcttgt ttcgagcaag
    23401 aggattttcc cttcccagtc tgacaagtca gggaaaagtt tgtatttttg gcagagctcc
    23461 gtgctatctg cgtatcgaga gttgtcaaag atatcgtaga gggattcgag gcaaccaccg
    23521 aggattttcc cctcgaattg ggcccttcct tgcaacaagt caaaacctgt atttgtatga
    23581 ctgacacgag gtgttcccag ggccgtggga ctaaaatcag ttcgttcctc ataccaaacg
    23641 tcactagggc ggatttctga aattcttccc gtctcaatca attctttaaa gtagtgaagg
    23701 ctataggcta gcatttcttt gtctaattca caaatgtctg ctaaaaagga ttgaccataa
    23761 aaagtcttga ttcctaattt atgcaacatg aggtggttca tggttgtatc cgagaagcca
    23821 agaaaaattt tttgtttgat aaccttttgg agttggtcat tttcaaaaag ataaggtagc
    23881 aagcgatagg tatcgtctcc accgatggca cataggatca tgtcgatgct atcatcagaa
    23941 aaggcatgaa tcaaatcctc tgcacgagct tcaggatggt ccttgataaa gtctaatcct
    24001 tttaacgaat ggggcaaaaa gatgggattg agtcccagat ccttgagacg ttggataccc
    24061 aagtccactt cgtgtttgac aaaatcctct ccgataatgc cactagataa actaacaata
    24121 ccaatagtag aaaccatatc tcatcctcct agaaatagat tgagcctatt ttatcacaaa
    24181 atataagaga aagctatgtg ggatttcaga agaatacttt taatactctt cgaaaatctc
    24241 ttcaaatcac gtcagtttta tttgcaacct caaagctgtg ctttgagcaa cctgcagcta
    24301 gcttcctagt ttgctctttg attttcattg agtataactc aaaaaagtaa gtgaaaaagc
    24361 aaccgcacct gcagttgctt ttatcgtttt cttattcttg atagcgtgtt ggtcctgcac
    24421 ttgcactacg gatattcaag cgtttcttga gatagaagcg aagggctagg agggctgctc
    24481 caataatagc tagtggcaat ggatccagta ctgggttaag gctagctggt aggaagcttg
    24541 ttgcaaagaa gacaagcaac caaaggaaca tagaagctag gataactagt acagatttcc
    24601 agaaaggtgg acgttgactg cgatccatat ctggtccata gtattggtaa acaaagtagt
    24661 acatcaagta gaaggcaaat ccaccaacca gtccaactaa tagaagagta atcaatccat
    24721 agccgaaagc ttgatctgcc gcaaagaagg ttgtgagggc gctgacaagg gcaaagaggc
    24781 tagtgatgaa aagagctgag tccataatca tcagttttgg gtcatcattt tcttttggat
    24841 gctctttttc gtactgctct ttgacagtga agctatgagc ccaatgagtt ggtgcgccgt
    24901 atagggaacg ggcagttgta cctttagatt gctcctcaag gatttgggga atgacttcct
    24961 caaaaatagt ctggatttca gcgtctgttt tcccatcttt gatgaattgt tgggtagcaa
    25021 tgtggacaaa ctcttggttt ttcttagtta atttttgtag atcaatttga gacataggaa
    25081 ctcctcttag aaccattttt tatggatgag atagagagtg agcgagacac tcatagcaaa
    25141 ggcgataaag acgattaacc agaaggcatt tggctctccg tttaggggaa tttcattatc
    25201 cttaaagttc atcccgtagg cagaaaagac catggttggg atggacatga cgatggtcac
    25261 aagggccaag gttttcatga tgttgttctg gttgttagaa atgatagagg caaaggtctc
    25321 tgtcatagaa tgcaagacgt ttccataaat atctgccatc tcgatggcct gttgggtttc
    25381 aatcagggtg tcttcaagca ggtcttcgtc ctcaaggtat ttcttgatat tgctggttga
    25441 actggtcaat ttcttaatca cgcgctcatt tgttttgagg gaggccttga aatagacgat
    25501 agttttttcc aattccatga gctcaatcaa ttcttcatta cgagttgatt gatgcagttg
    25561 actttcgatt tgttcactct tgcggtcgat tgaacgaagg gctgttaggt aaagctctgc
    25621 attgcgataa agaatttgaa agataaaacg tgaacgcatg aaggtataga aattacgcaa
    25681 tcgacggttg ataaagacat caaggacagg tagtggttcc aaacacgtag tgataatggt
    25741 ttcctcagtg atgataatac caagcgggat ggttacgtag taggtgcggt tatttctttc
    25801 ctccgtgacc ggcacgtcta cgataatcag ggtatactcg tcttcaatgg taatacgaga
    25861 catttcttcc gcatcgagcg gtgctcgaag gtcggcaata tcaatatcga aggtgttggc
    25921 gatttcgagt gattcatttt gagtcggatt gacgagattg atccaagtac ccggttcaag
    25981 cgtatcgatc tctttaaatt cagttgttgt agagagaaaa acttgtttca tatcccttat
    26041 cctttctcat ttttcagatt ttttcacact gtactattat actacaaaat cggccttttg
    26101 ggtaatagaa tctcactttt tttggtataa tggtaagcaa taatggacta gaaagaacaa
    26161 agatgcaaga taaaattgtc attcatgggg cgcgtgccca taatttaaaa aatattgatg
    26221 tggagattcc gcgagacaag ttggttgtcg tgactggctt gtcaggttca gggaagtcca
    26281 gtctggcttt tgatactctc tatgcggagg gacaacgtcg ctatgtagag agtttgtccg
    26341 cctacgctcg tcagttcttg ggaaatatgg aaaaaccaga tgtagatgcc attgatggtc
    26401 tcagcccagc tatttccatc gaccagaaaa cgactagtaa aaatcctcgc tcgacggtgg
    26461 gaacgacgac tgaaatcaat gactatctgc gtctcctcta cgcacgtgtg gggacgcctt
    26521 actgtatcaa cggacatgga gctatcaatg cttcttctgt ggagcaaatc gttgataagg
    26581 ttttggagtt acctgaaggc cagcgcttgc agatcttggc tcctgtcatc cgcaagaaaa
    26641 aaggccaaca taagagtgtt atcgagaagg ttcagaaaga cgggtatgtt cgtgttcgtg
    26701 tggatgggga agtctatgat gtgaccgaag tgccagagtt gtctaagagc aagcaacaca
    26761 atatcgatgt cgtggttgac cgtattgtta tcaaggaggg cattcgtagt cgtctctttg
    26821 attccattga ggctgccctt cgtattgcag aaggttatgt cattatcgac accatggacg
    26881 attcggagtt gctcttctct gagcattatg cctgtccagt ttgtggtttt actgtcccag
    26941 agttagagcc tcgtctcttc tctttcaatg ctccttttgg ctcttgtagt gagtgtgacg
    27001 gcttgggcat caagctggag gtggatactg atttggtagt gccagatgcc agcaaaacct
    27061 tacgtgaggg agcgctagca ccttggaatc ctatctcatc caactactat ccaaacatgt
    27121 tagagcaggc catgaaagtc tttggagtgg ctatggataa gccttttgag gacctgtcag
    27181 aagaagataa gaacttgatt ctctatgggt cagatggtaa ggaattccat ttccactatg
    27241 agaatgaatt tggtggcgtg cgcgatatcg acattccgtt tgagggagtt atcaataata
    27301 tcaaacgtcg ttaccatgaa accaatagcg attacactcg cactcagatg cgtctctaca
    27361 tgaatgagct gacctgcgga acctgtcaag gctatcgtct caatgaccag gccttgtctg
    27421 tccgtgtggg cggccagcaa gggccacata ttggagaaat ctcagacctg tctatcgctg
    27481 accacttgga cttggtgagc cagttaactt tgtctgaaaa tgaagccatc attgctcgtc
    27541 ccattctcaa ggaaatcaag gatcgtttga ccttccttaa taatgtgggt cttaactatc
    27601 taaccctgtc acgttcagca ggaacccttt caggtgggga aagtcagcgc attcgtttgg
    27661 caacccagat tggttccaac ctatcaggcg tcctctatat cctagacgag ccgtcaatcg
    27721 gtcttcacca gagggacaat gaccgcctga ttgccagtct gaaaaagatg cgtgacttgg
    27781 gcaatactct tatcgtggtg gaacacgacg aagataccat gcgtgaagct gattatctga
    27841 ttgacgttgg tcctggtgcc ggtgtttttg gtggtgagat tgttgcagca ggtacaccta
    27901 agcaagtggc tcgcaacagc aagtctatca caggtcagta cttgtcaggc aaacgtgtca
    27961 tcccagtatc agaagagcgc cgtgtcggaa atggtcgttt tattgaagtg ataggagcgc
    28021 gtgagaacaa cttgcaaaat gtcacggctc gctttccact aggaaaattt atcgcagtga
    28081 caggtgtgtc gggttcaggg aaatcgactc taatcaacag cattctcaaa aaagccattg
    28141 cccagaagct caaccgtaat tcagacaaac ctggtaagtt taagactatt acagggattg
    28201 agcatgtcga ccgtttgatt gatattgacc agagccctat cggacggacg ccgaggtcta
    28261 acccagctac ctatacagga gtttttgacg atatacgtga cctttttgct cagacaaatg
    28321 aagccaagat tcgaggctac aagaaggggc gcttcagttt caacgtcaag ggaggtcgct
    28381 gtgaagcttg ctcaggtgac gggattatca agattgagat gcacttcttg ccagatgttt
    28441 atgtggcttg tgaagtttgc cacggaaccc gctataacag tgaaacccta gaagttcact
    28501 acaaggaaaa gaatatttcg caggtcttgg atatgacggt caacgatgcg gtagaatttt
    28561 tccaacacat tccgaaaatt caacgcaaac tccagaccat caaggatgta gggctaggct
    28621 atgtgacctt gggacagcca gctaccaccc tttctggggg agaagcccaa cgtatgaagt
    28681 tagctagcga actccacaaa cgctcgacag gaaaatcttt ctacattctg gatgagccga
    28741 cgacagggct tcatacagag gacattgctc gcctgctcaa ggttttagct cgctttgtag
    28801 acgatggcaa tacagtcctc gtcatcgagc acaatctgga tgttatcaag acggcagacc
    28861 atatcattga cttgggacct gagggcggtg tcggtggtgg aaccatcatc gtaacaggaa
    28921 ctccagaaga agtagctgcc aacgaagcca gctatacagg acactatttg aaaggaaagt
    28981 tacatcatga ataaacgcgt acaagcattt ctagctaaaa tgcaagaaaa agaactagat
    29041 ggtatcatca tcaacaatct taaaaacgtc tattatttga ctggtttttg gggctcaaac
    29101 ggaacagtct ttatcagccg tgaccgtcag gtcttagtga cagactctcg ctatatcata
    29161 gcagctaagc aagaaaccag tggttttgag attgtggctg atcgtgatga attggctgtc
    29221 attgcaggaa ttgttaagga catgggcttg actcgtatcg gttttgaaga tgagatttca
    29281 gtgtcttatt accaccgtat gcaggcagct tttgcaggtt tggacttgct tccacaaact
    29341 cagtttgtgg aaggtcttcg tatgattaag gatgaggcag agattgcagc gattcgcaag
    29401 gcttgttcta tctcagacca agctttccgc gatgcgcttg actttattaa accaggaaaa
    29461 actgaaattg agattgccaa cttccttgac ttccgcatgc gtgagttggg agcatctggc
    29521 ttatcttttg atacgatcct agctagcggt atcaattctt ctaaacccca tgcccatcca
    29581 atgcacaaac cagtggagtt gggagaagcc attaccatgg acttcggctg tctctatgac
    29641 cactatgtca gtgatatgac ccggactatc tatctagggc atgttagcga tgagcaggca
    29701 gagatttaca atacggttct aaaagctaac caagccttga ttgaccaagc taaggcaggc
    29761 ttaggtttcc gtgactttga caaaatccct cgtgatatta tcattgaggc aggttatggc
    29821 gactacttta ctcacggcat tggccacggt attggtctgg atatccatga ggaaccctac
    29881 ttcagccaga cttctacaga aactattaag acaggtatgg ccttgaccga tgaaccaggt
    29941 atctatatcg aaggcaaata tggcgttcgt atcgaagatg atatcctgat tacagagaca
    30001 ggttgtgaat tattgaccct agctccaaaa gagttgatag tcatttagtt gtttgtcaag
    30061 aaaaaagtac gaaaatgacg aaaaaagtca aaaaaaatta aaaataggtc gcaagtcgga
    30121 tgttttttat ggtataatag actaaactga tagtaacatg tagcgaaagg ggtaggtaca
    30181 tgattaaaat ttatacagtc tcaagttgta ctagctgtaa aaaagcaaaa acctggctca
    30241 atgcccacca gttaagttat aaagaacaaa accttggtaa agaaggaatt acgagagaag
    30301 aattactgga tattctaacc aaaacagata acggaatagc cagcatcgtt tcgtctaaaa
    30361 atcgctatgc caaagccctt ggagtggata ttgaagattt gagtgtcaat gaagttctca
    30421 atctgattat ggaaacaccg agaattttaa agagcccaat ccttgtagat gaaaaacgcc
    30481 tgcaagttgg ctacaaggaa gacgatattc gtgccttcct accacgctct gtccgtaatg
    30541 tagaaaatgc agaagcacgt ttgcgtgcag ctctataaac atcaaggctg ggagcacttc
    30601 ccagtcttat tctattttaa tttcaaaaag aaagaagaaa gaaatgaaaa aaatagttct
    30661 tgttagtcta gctttccttt ttgtcctggt tggttgcgga cagaaaaaag aaactggacc
    30721 agctacaaaa acagaaaaag atacgcttca gtcggcattg ccagttattg aaaatgctga
    30781 gaagaataca gttgtaacta agactttggt cttgcccaag tcagatgatg gtagccagca
    30841 gacacaaaca attacttaca aagacaagac ttttttgagt ctaactatcc aacaaaaacg
    30901 tccagtctct gatgagttga agacttatat tgaccaacat ggagtggagg aaactcaaaa
    30961 agctcttctt gaagcggagg agaaggataa gtctatcatt gaagctcgta aattggcagg
    31021 tttcaaactt gaaacaaaac tattgagcgc aacggaactt caaacaacga ctagttttga
    31081 ttttcaagtt ctggatgtca agaaggcttc ccagttggaa catctgaaga atattggttt
    31141 ggaaaatctt ttgaaaaatg aaccaagcaa atatatttca gatagattgg caaatggcgc
    31201 gacagaacaa tagaaaatcc aaataaatag actgcctgaa ttgtagaagg taaggaatta
    31261 tgctataatg gtactattct aaggaaagag ggtgttagaa tgggatttac tgaagaaaca
    31321 gtacgtttta aattggacga ttccaataaa aaagaaatta gcgaaacttt gacagatgtt
    31381 tatgcttcgt tgaacgataa gggttacaac ccaattaacc aaatcgtagg ttacgtattg
    31441 agtggagacc ctgcctacgt tcctcgttat aataatgcac gaaatcaaat ccgtaagtat
    31501 gagcgtgatg aaatcgttga ggaattggtt cgctactacc taaaaggaca aggagtcgat
    31561 ctataaccta tgagaattat gggattggac gtcggttcaa aaacggtagg ggtggcgatt
    31621 agcgatccgc ttggttttac agctcaaggg cttgaaatca tccagataaa tgaagaacaa
    31681 ggccaatttg gttttgaccg cgttaaggaa ttggttgata cttacaaggt ggaacgattt
    31741 gtagtgggct tgcctaaaaa catgaacaat acaagtggac cgcgcgtaga agctagtcaa
    31801 gcctacggag caaagctaga agagtttttt ggtttaccag tagactatca ggatgaacgc
    31861 ttgacaacag tggctgctga gcgcatgttg attgaacaag cagatatcag tcgcaataag
    31921 cgcaagaaag tcattgataa gttagcagct cagctgattt tacaaaatta tttagataga
    31981 aaattttaat ataaaggaga ggctatgtca cacgatcata accacgacca cgaagaacgt
    32041 gaactaatca cactagtaga tgagcaagga aatgaaacct tgtttgaaat ccttttgaca
    32101 attgatggaa aagaagaatt tggtaaaaac tatgttcttc tagtaccagt taacgcagaa
    32161 gaagacgaag acggacaagt tgaaatccaa gcttactcat tcattgaaaa tgaagatgga
    32221 acagaaggcg aattgcaacc aatcccagaa gactcagaag acgaatggaa catgattgaa
    32281 gaagtcttca acagctttat ggaggagtga tacagtctgg gagactgtat cagcccaact
    32341 ccagaaatgg ggaagagttg gaacgtccag tgaacatttt taccccaact cttagaaatt
    32401 gagaagagtt ggaacatcta gtggatgttt ttagcccata ttcacattca tagtagctag
    32461 ttattagcta accaggtaag aggtcgggac gaaaatcctg gccttgtttt tattagtaac
    32521 catactttaa tgcgataatt gattggactt ttgtcaagga gatgtgtatg tttgaagtag
    32581 aagaatggct ccatagtcgg attggtttga attttcgatc aggtttgggc cgaatgcagc
    32641 aagcggtgga tttgttagga aatcctgagc agtcttaccc tattatccac gtaacaggga
    32701 ctaatgggaa aggatctacc attgctttta tgagggaatt atttatgggg catggcaaaa
    32761 aagttgcgac ctttacctcc cctcatatcg tctctatcaa tgaccgaatc tgcattaatg
    32821 ggcaacctat agcagacgca gactttatcc gtttgactga tcaggtcaag gagatggaga
    32881 aaacgcttct gcaaactcct gcccagttgt ccttttttga attgctgacc ttagttgctt
    32941 ttctttattt tagggagcaa gaggtggatt tggttttatt agaagtggga attggtggct
    33001 tacttgacac gaccaatgtg gtaactggag agcttgctgt catcacctcc attgggcttg
    33061 accatcaaga aaccttgggt gatagtctag aagcaattgc agagcagaaa gctggtattt
    33121 tcaaggctgg taagaaggca gtgattgcga aattgcctcc agaagctagg cttgtctgtc
    33181 agaaaaaagc cgaatcttta gctgttaacc tttatcaggc aggtcaagat tttttaatgc
    33241 tgaatggtga tttttcaagc tctttactaa atctttcaca gctgaacata ggcttagaag
    33301 gagtctatca gcaggagaat gcagccttgg cgttgcaaac ttttcttctt tttatgggag
    33361 aaggaaagga agctgttgat gaacaggctg taagaaaggc cttggaacag acccattggg
    33421 ctggtcgctt ggagcgtatt cgcccacaga tttacttgga tggtgctcat aacctccctg
    33481 ccttgactcg cttggttgag tttatcaaag aaaaagagca ggaaggctat cgacctcaaa
    33541 tcctttttgg agccttgaaa cggaaggatt atcaagggat gttaggttac ctgactgaaa
    33601 aattgcctca ggtggaactc aaggtgaccg gctttgacta tcagggggct ttggacgaaa
    33661 gggatgtgac aggttacgat atagtttctt cttaccgaga atttatcagc gattttgaag
    33721 aaagagcaga tgctcaggat ttgctgttcg ttacagggtc tctctatttt atctcagaag
    33781 tacggggcta cctgctggac cgtgagcaga taaattgacc cccttgtttg aacttgttat
    33841 actaggggaa ttgctggtta gagaaaattt ctctaaattg gtagcagaaa ggaaattcat
    33901 catgaaatta aaaagattca cactttctct tgcttctcta gcaagtttta gtctcttagt
    33961 agcttgttca caaagagctc aacaggttca acagcctgtt gctcagcagc aggtccaaca
    34021 gcctgctcaa cagaatacca atactgcaaa tgcaggaggt aaccaaaatc aagcggctcc
    34081 agtacaaaac caacctgttg ctcaaccgac cgatattgat gggacttata ctggtcagga
    34141 tgacggagac cgtatcactt tagtggtaac tggaacgact ggtacatgga ctgagctcga
    34201 atctgacggg gatcagaaag tcaaacaggt tacatttgat tcagcaaatc aacgcatgat
    34261 tattggcgat gatgtcaaaa tttacactgt aaacggtaat caaatcgtcg tagatgatat
    34321 ggatagagac ccatcggacc aaatcgtttt aactaaataa gactatgcaa gtagaaaggg
    34381 ctggattttt cagccttttt acttttacct agaaaataat cataaatact tgccttctac
    34441 cgaatacctg cgttatacta gtatcaatta cttgttttta gcattcaaac tatcaaggag
    34501 gggatatgaa atataggaaa tttcaattat tgatgtccaa gtatggcttt agtctttcga
    34561 ttatgctact tgaactttgt cttgtttttg gtctctttct ttatttagga cgcatggctc
    34621 ccattttatg gattactgtc ctcattctac tgagtatcat cacaatcatt tcgatagtca
    34681 accgtaatac gactcctgag aataaggtaa cctggttgtt agtagccttt gtgccagtat
    34741 ttggtccctt gctctatctg atgtttggtg aaaggcgatt gtccaaaaaa gaaatcaaac
    34801 aactgaagaa gctaggctct atgcatttcc aagaagcaaa tagccagcta ctaaaagaga
    34861 aattaaaaga aagtgacaag gcagcttatg gagtcatcaa gtccttattg agtatggata
    34921 ccaatgctga catctatgat caaactgcct ctacattttt tcctaacgga gaagctatgt
    34981 ggaaaaagat ggtagaagat cttaaaaagg ctgagaaatt tattttcttg gaatattaca
    35041 ttatagaaga aggtttgatg tggaatcgca tactagatat actagagcaa aaggtagctc
    35101 agggtgtaga ggttaagatg ctctatgatg atatcggctg tatggctact ttaacaggag
    35161 attatgcaca tcgacttcgt cagctgggca tcgaggccca taaattcaat aaagttattc
    35221 ctcgtttgac agtggcttat aataacagag atcatagaaa aatattgatt gttgatggtc
    35281 agatagccta tactggtggg gtcaatctgg cagatgagta cattaaccac gtcgagagat
    35341 ttggttattg gaaggatagt ggaattcgct tagacggact agcagtaaaa gctctgacac
    35401 gcttattttt gaccacttgg tacattaatc gaggagaaat tagtgatttt gatcaatatc
    35461 atttagaaaa tcattctatc ccgagtgacg gtttaaccat tccatacgga agtggaccca
    35521 agccaatttt tcgagcgcag gtagggaaaa aagtttatca gagtttaatc aatcaagcaa
    35581 cagaatcggt ctatattacg acaccttatt tgattataga ttatgattta acagagacaa
    35641 tcaaaaatgc agctatgaga ggggtcgatg ttcgaattat caccccttac ataccagata
    35701 agaagttcat tcagttagtc acgagaggag cttatcccga ccttctttct gctggtgttc
    35761 ggatttatga gtatagtcca ggttttattc atagtaagca gatgttggta gacgaagatt
    35821 ttgcggtggt ggggacaatc aatctcgact accggagctt ggtacaccat tatgaaaatg
    35881 cagtcttact ctataaaact ccttctataa gggaaatcgc ccgagatttt cgaaatatat
    35941 ttgcagattc tcaggaagtc tatcctcatt ctatcaaaac gagctggtat caaaagcttg
    36001 taaaagaaat cgcccagcta ttcgccccta tcttataaga aatctagaac ttaggataca
    36061 aaccagacct atttttcttg tcttttacga atagaaagaa agaggagggg aattcatgct
    36121 aactaaggaa gaagtgaatg atatgattga gttcaaacag acacatttac accgctttcg
    36181 ggaaattgaa acctttgtga aactatgcaa aaaaatcact caaacagcca tcgcaagctg
    36241 acaatcctag gactttttga tatactaaga cagataaatt gaacaggaga aacgatatga
    36301 gtgtgtatgg tagagtagaa gaagttcata aggagaatcg tgaacctcta gaataccaaa
    36361 tcgaacaaga atcgcatcat cgtgaatcaa gtcgtcttcc tttggtgaaa attttactat
    36421 ggagtacgct tgtaacgggg ataactctag gagtaccgct attactcgac ttaatgagtg
    36481 cacaagaagt gcaggatttt tatgcaggtt gggcgcttca tcagacaggg aagatttaca
    36541 gcgactatta tggaagtcaa ggtttgcttt attatttgct gacttacgtg agtcagggcg
    36601 gatttttctt tgccatcttt gagtggttag ccttggtagc aggaggattt ttccttttta
    36661 gatcagcgga caccttgaca gagcaaggag accaagctgg acatctggtg actatttttt
    36721 acatgctagt tacaggtctt gcttttggtg gaggctatgc gactctttta gcgcttcctt
    36781 tcttattcgc agcctttagt ttagttgcgg cttacctaag caatccaagc catgataagg
    36841 gatttgtacg gattgggcta gctttggcag gcggattttt ctttgctccc ttatcatcgc
    36901 tcctgtttat tgctgtagtg agtttaggct tgttggtctt taaccttggg catagacgct
    36961 ttgcgcatgg gttttatcag tttcttgcag tggctttagg tttttcactt gtcttttatc
    37021 caactgccta ctatagtgct gcaacaggaa gttttgggga tgcgattagt ggtattcgtt
    37081 atcctattga cagtattcgc tttgatttta cttctaaaat tttagagaat atgttttttt
    37141 atggcttgtt gtcccttggt ttgggatttg tggtttgtat ctttttaggt ctctttcaat
    37201 ccaaaccctt taaactatac gtcatttcgg ttcctgcaag tcttgtggta attttaggtt
    37261 tgattttact tttcttttca caagagcctc tgcacgcttc ttatttgatg gtcgtcttcc
    37321 ctgttttcct acttttattg gtaaccaata ttaagagtca acagaggggg cgtagtgcta
    37381 gaagaagccg aagagaaacg ccagttagcc tatggagtcg tttcttcaaa ggaaatctat
    37441 atctgctagt ttttgggttt gtctatcttt tgtctgttcc ttttttgatg aagtttgtcc
    37501 tttatccagt accttatcaa gaacgtaatc gtcttgctga tttggtaaaa gaggagacaa
    37561 atacggaaga tgctatctat gcatgggatg atactgcgac tctttatcgt aagagtgagc
    37621 gcttgtcgcc atcggcgatt ttgtccccgt tgcactatac agcaactgag gaaaatcgta
    37681 ataagttact taatgacttg aaagaaaaac aacctaaggt gattgtggta aatgataagg
    37741 tggtagtctg gtctgaagtg gaaacactct taaaagaaaa ttaccaacaa gtaaagactg
    37801 attactcaga gtttaaagtc tataaaatta aataaccaaa tcaatatctt gtgtattttt
    37861 aaaaatttta ggatttttaa cacaagatat tgatttttct ttttagagtg gtataatact
    37921 ttttagaaag aacattttag aaaagagcat gcatatgatt gcactagaag aaaaaattac
    37981 aattttgcca actctcttcg tcgagaaacg agatgggaga cgtgttgtat ttgatgtgga
    38041 caagattgac aaggctctcc acaaggcggc tgacaaggtt atggatgtga cacccctggt
    38101 tgaaaaacgc ctcaatgatc tgactgagcg aattattaca gaaattcata gtcgctttcc
    38161 acagggaatt aagatttacg aaattcaaaa tatcgtagaa catgaactcc ttgaagccaa
    38221 agaatatgcg ctggctgagg agtatattac ttatcggaca cagagggatt ttgagcgctc
    38281 aaaagcgacg gatatcaact ttagtattca taaacttctc aacaaagacc agacagttgt
    38341 caatgaaaac gctaataaag acagtgatgt ctttaacact cagcgtgatt tgacagcagg
    38401 gattgttggg aaatcaatcg gactgcaaat gcttcctaag cacgtagcca atgcccacca
    38461 aaagggggat atccactatc acgatttgga ctacagtccc tataccccta tgaccaactg
    38521 ctgtttgatt gattttaagg gtatgttgga aaatggtttt aagattggaa atgcagaggt
    38581 agagagtccc aagtctatcc agactgcgac agcacagatt tctcaaatca ttgccaacgt
    38641 tgcttctagc cagtacggtg gctgttcagc tgaccgtatc gatgaaattt tggcgcctta
    38701 tgcagagaag aattatcaaa aacatctcaa agatgcagaa gagtgggtat tgcctgaaaa
    38761 acaggaagat tacgcttgga agaaagcgca aaaggacatc tacgatgcca tgcaatctct
    38821 tgagtatgaa atcaatactc tcttcacttc aaatggacaa acacctttta cttcgttagg
    38881 ttttggtctg ggaaccagtc gttttgaacg agaaattcaa aaagctattt taaacattcg
    38941 catcaagggt cttggttcag aacaccgtac ggctatcttt cctaaactta tctttacgct
    39001 taaaagaggc ctcaacttag aggaaggaac tcccaactat gacatcaagc agttggctct
    39061 agagtgtgca accaagcgga tgtatccaga cgtcttgtct tatgataaga ttgttgattt
    39121 gacaggttct ttcaaggtgc ctatgggctg ccgttctttc cttcaagggt ggaaggatga
    39181 aaatggtgta gaagtcaatt caggtcgcat gaatctgggt gttgtgacgg ttaatctgcc
    39241 tcgtattgct cttgagtctg aaggtgatat gaataagttc tgggaaatct tcaacgagcg
    39301 aatgaatatc gcagaagatg ctcttgttta ccgtgtcgaa cgcactaaag aggcgacacc
    39361 agcgaatgct cctattcttt atcagtacgg tgcttttggc catcgtctag gtaaagaaga
    39421 aagtgttgac cagctcttta agaatcgtcg tgcgaccgtt tcgctgggct atatcggctt
    39481 gtatgaagta gcgacagttt tctttggtaa cagctgggaa agtaatccag atgctaagga
    39541 attcacgcta gacatcattc acgatatgaa acgccgtgta gaagagtggt cagaccaata
    39601 tggctaccat ttctctatct actcaacacc atccgaaagt ctgacagacc gtttctgccg
    39661 actagatata gacaagtttg gctctattcc tgatatcaca gacaaggaat actacaccaa
    39721 ctctttccac tacgatgttc gtaaaaatcc aacaccgttt gaaaaattgg actttgagaa
    39781 agtctatcca gaagcaggtg cgtcaggtgg tttcatccat tattgtgagt atccagtcct
    39841 tcagcaaaat ccaaaggcct tggaagctgt ctgggattat gcttatgacc gtgtaggcta
    39901 tctaggcacc aatactccga ttgaccgttg ctacaagtgt gactttgaag gggattttga
    39961 accaactgag agagggtttg cttgtccaaa ctgtggcaat agcgacccta aaacagtaga
    40021 tgtggtgaag cgaacttgtg gctacctagg taatcctcaa gcaagaccga tggtcaacgg
    40081 gcgtcacaag gaaatcgctg cgcgtgtcaa acatatgaat ggttcaacga ttaaaatagc
    40141 tgggcatcaa gtaacaaatt agaaagaaat gaaatgggaa aatatcaact agacgataag
    40201 gggcgcgcac aagtgacccg ttatcacgag aaacactcta aaggtggagc tggtaagaaa
    40261 gaacgcttgc ttagcttcag agaacaattt ttaaacaaga acaagaaaaa ataaaagtga
    40321 gagccagctc tcgcttttct catagtggga ggtaaggatg gaattacgca gaccaagatt
    40381 agcggataag aaagctgttt tagatatgat gacagagttt gaaaaatttc agtcgcctca
    40441 cgacggcggt ttctgggata cagagaactt tgtgtatgaa gactggttag aaagcaatca
    40501 ggaacaggaa atggggatta atctgcctga aggatgggtt cctgcaattc agttagtggc
    40561 tttttctgag aaaggtcaag cagttggatt tcttaatctc cggttgcgcc tcagtaactt
    40621 tttactagaa gaaggtgggc acattggcta ctccatccgt ccatctgaaa gaggcaaggg
    40681 ttatgcaaaa gagactctcc gtcagggctt gcaagttgct aaggaaaaga acatcaagaa
    40741 agctctggtg acctgtagcg tgaataatcc tgctagcaga gcagtcattc tagcaaatgg
    40801 tggaatattt gaggatgctc gcaatggagt cgagcgttat tggatagagg tagcgaatga
    40861 ataatccaaa accacaagaa tggaaaagcg aggaacttag tcaaggtcgt atcattgact
    40921 acaaggcctt taactttgtt gatggcgaag gcgtgcgtaa ctctctctat gtatcaggct
    40981 gtatgtttca ctgcgaggga tgttataatg ttgcgacttg gtcttttaat gctggcattc
    41041 cctatacagc agaattagaa gagcagatca tggcagacct tgcccaaccc tatgttcaag
    41101 gcttgacttt gctgggaggg gagccttttc tcaatactgg cattctcttg cctctagtta
    41161 aacgcatccg aaaggaattg ccagacaagg acatttggtc ctggacgggc tacacttggg
    41221 aagaaatgat gttggaaact ccagataaac tggaactctt atcattgatt gacattcttg
    41281 tcgatggacg atacgatcga actaagagaa atctcatgct ccagtttcga ggttcgtcca
    41341 accaacgaat tatcgatgtg caaaaatcgc tcaaaagtgg gcaagtagtg atttgggaca
    41401 agctcaatga cggaaaagaa agctatgaac aggtgaagag agaatgaaga aaaaagactt
    41461 agtagaccaa ctagtctcag cgatcgagac ggggaaagtc aggacactgg gaatatacgg
    41521 tcatggagct tcaggtaaat caacctttgc acaggaattg taccaagctt tagattctac
    41581 tacagtaaat ttgctagaga cagatcctta tatcacctca ggacgccatc tggtagtacc
    41641 caaggacgcg ccgaatcaaa aggtgacagc cagtctgcca gtggcgcatg aactggagag
    41701 tttgcagaga gatatccttg ccttgcaggc gggtatggat gtcttgacaa ttgaagaacc
    41761 ttggaaggct agtgaggtct tgtctggagc caaaccaatt ttgattgtcg aagggatgtc
    41821 tgttggtttt ctacccaagg aactctttga aaaaaccatc tgtttctaca cggatgagga
    41881 gaccgaatta aagcgacgcc ttgctagaga tacgactgtg agaaatcgcg atgcatcctt
    41941 tatattggct agccatcaga tgagacggga gcagtatctg cgatactata aagaaactga
    42001 gtctaaggcg gatatcttag tggactaatc agaagataaa tttgatgtca agaggactta
    42061 acttatgtat agaagaaata tagaagaaaa tccctaattt tagaaagaaa aagtaggaaa
    42121 acagtttcat cccaaaaaaa cgaaaaaaca gcaacaaatc ccttgcaatc gcaggggatt
    42181 tgtgttattc tattatggtg ctgtaaatta cagccttagt tttgatgcaa gaggttgcga
    42241 cacgctcggt tgcattgcca cgcaacaccg tgtcggtttt cttgtggagc tagcctatta
    42301 tcttaaatag acgaaaagga gaaaaagatg gcaaacaaaa aaatccgtat ccgtttgaaa
    42361 gcttacgaac accgtacgct tgacacagcg gctgcaaaaa tcgtagaatc agctactcgt
    42421 acaggtgcac aagttgcggg tccaatccca cttccaactg aacgtagcct ctacacaatc
    42481 attcgtgcga ctcacaaata caaagactct cgcgaacaat ttgaaatgcg tacacacaaa
    42541 cgtttgatcg atatcgttaa cccaactcaa aaaacagttg atgccttgat gaaattggat
    42601 cttccaagtg gtgtaaacgt agaaatcaaa ctttaatcta aaatataaaa gagcagaggc
    42661 tggtgtttca atctaattga acacgggcta aactcggtgt gaaaaagata aacttcctag
    42721 tgtctgctag acactgcgtc agtttcctat tttcacgttg agtttgacgc cctttgtatc
    42781 ttagacttga gcataaaaaa cgctcgttaa aaactttttg aataaaaaat atagaaaagg
    42841 aactattttc tcatgacaaa aggaatctta gggaaaaaag tgggaatgac tcaaatcttc
    42901 actgaagctg gcgaattgat ccctgtaaca gttattgaag caactccaaa cgttgttctt
    42961 caagttaaaa ctgttgaaac agacggatac aacgctatcc aagttggttt cgatgacaaa
    43021 cgcgaagtat tgagcaacaa acctgctaaa ggacatgtag cgaaagctaa cacggctcct
    43081 aagcgcttca ttcgtgaatt caaaaacgtt gaaggcttgg aagttggtgc tgaaattaca
    43141 gttgaaacat tcgcagctgg agacgttgtt gacgtaactg gtacttctaa aggtaaaggt
    43201 ttccaaggtg ttatcaaacg ccacggacaa tcacgtggac caatggctca cggttctcgt
    43261 taccaccgtc gtccaggttc tatgggacct gttgcaccta accgcgtatt caaaggtaaa
    43321 aaccttgcag ggcgtatggg tggcgaccgc gtaacaattc aaaaccttga agttgtacaa
    43381 gttgttccag aaaagaacgt tatccttatc aaaggtaacg taccaggtgc taagaaatct
    43441 cttatcacta tcaaatcagc agttaaagct ggtaaataat aaagaaaggg gaaatcagtc
    43501 acaatggcaa acgtaacatt atttgaccaa actggtaaag aagctggcca agttgttctt
    43561 agcgatgcag tatttggtat cgaaccaaat gaatcagttg tgtttgatgt aatcatcagc
    43621 caacgcgcaa gccttcgtca aggaacacac gctgttaaaa accgctctgc agtatcaggt
    43681 ggtggacgca aaccatggcg tcaaaaagga actggacgtg ctcgtcaagg ttctatccgc
    43741 tcaccacaat ggcgtggtgg tggtgttgtc ttcggaccaa ctccacgttc atacggctac
    43801 aaacttccac aaaaagttcg tcgcctagct cttaaatcag tttactctga aaaagttgct
    43861 gaaaacaaat tcgtagctgt agacgctctt tcatttacag ctccaaaaac tgctgaattt
    43921 gcaaaagttc ttgcagcatt gagcatcgat tctaaagttc ttgttatcct tgaagaagga
    43981 aatgaattcg cagctctttc agctcgtaac cttccaaacg tgaaagttgc aactgctaca
    44041 actgcaagtg ttcttgacat cgcaaatagc gacaaacttc ttgtcacaca agcagctatc
    44101 tctaaaatcg aggaggttct tgcataatga atttgtatga tgttatcaaa aaacctgtca
    44161 tcactgaaag ctcaatggct caacttgaag caggaaaata tgtatttgaa gttgacactc
    44221 gtgcacacaa acttttgatc aagcaagctg ttgaagctgc tttcgaaggt gttaaagttg
    44281 ccaatgttaa cacaatcaac gtaaaaccaa aagctaaacg tgttggacgt tacactggtt
    44341 ttactaacaa aactaaaaaa gctatcatca cacttacagc tgattctaaa gcaatcgagt
    44401 tgtttgctgc tgaagctgaa taatctaagg aggaaatatc gtgggaattc gtgtttataa
    44461 accaacaaca aacggtcgcc gtaatatgac ttctttggat ttcgctgaaa tcacaacaag
    44521 cactcctgaa aaatcattgc ttgttgcatt gaagagcaag gctggtcgta acaacaacgg
    44581 tcgtatcaca gttcgtcacc aaggtggtgg acacaaacgt ttttaccgtt tggttgactt
    44641 caaacgtaat aaagacaacg ttgaagcagt tgttaaaaca atcgagtacg atccaaaccg
    44701 ttctgcaaac atcgctcttg tacactacac tgacggtgtg aaagcataca tcatcgctcc
    44761 aaaaggtctt gaagtaggtc aacgtatcgt ttcaggtcca gaagcagata tcaaagtcgg
    44821 aaacgctctt ccacttgcta acatcccagt tggtactttg attcacaaca tcgagttgaa
    44881 accaggtcgt ggtggtgaat tggtacgtgc tgctggtgca tctgctcaag tattgggttc
    44941 tgaaggtaaa tatgttcttg ttcgtcttca atcaggtgaa gttcgtatga ttcttggaac
    45001 ttgccgtgct acagttggtg ttgtcggaaa cgaacaacat ggacttgtaa accttggtaa
    45061 agcaggacgt agccgttgga aaggtatccg cccaacagtt cgtggttctg taatgaaccc
    45121 taacgatcac ccacacggtg gtggtgaagg taaagcacca gttggtcgta aagcaccatc
    45181 tactccatgg ggcaaacctg ctcttggtct taaaactcgt aacaagaaag cgaaatctga
    45241 caaacttatc gttcgtcgtc gcaacgagaa ataatattaa actagtcgct taagcaacta
    45301 gtaaatccgc cagctcggta gcgctccata ggagtgcaag ccgctgtggt acaacattta
    45361 aaggagaaaa tataaaaatg ggacgcagtc ttaaaaaagg acctttcgtc gatgagcatt
    45421 tgatgaaaaa agttgaagct caagctaacg acgaaaagaa aaaagttatt aaaacttggt
    45481 cacgtcgttc aacgatcttc ccaagtttca ttggttacac tattgcagtt tatgacggac
    45541 gtaaacacgt acctgtttac atccaagaag acatggtagg ccacaaactt ggtgaatttg
    45601 caccaactcg tacttacaaa ggtcacgctg cagacgacaa gaaaacacgt agaaaataag
    45661 gagaacataa atggcagaaa ttacttcagc taaagcaatg gctcgtacag tacgtgtttc
    45721 acctcgtaaa tcacgtcttg ttcttgataa catccgtggt aaaagcgtag ccgatgcaat
    45781 cgcaatcttg acattcactc caaacaaagc tgctgaaatc atcttgaaag ttttgaactc
    45841 agctgtagct aacgctgaaa acaactttgg tttggataaa gctaacttgg tagtatctga
    45901 agcattcgca aacgaaggac caactatgaa acgtttccgt ccacgtgcga aaggttcagc
    45961 ttcaccaatc aacaaacgta cagctcacat cactgtagct gttgcagaaa aataaggagg
    46021 taaaatcgtg ggtcaaaaag tacatccaat tggtatgcgt gtcggcatca tccgtgattg
    46081 ggatgccaaa tggtatgctg aaaaagaata cgcggattac cttcatgaag atcttgcaat
    46141 ccgtaaattc gttcaaaaag aacttgctga cgcagcagtt tcaactattg aaatcgaacg
    46201 cgcagtaaac aaagttaacg tttcacttca cactgctaaa ccaggtatgg ttatcggtaa
    46261 aggtggtgct aacgttgatg cactccgtgc aaaacttaac aaattgactg gaaaacaagt
    46321 acacatcaac atcatcgaaa tcaaacaacc tgatttggat gctcaccttg taggtgaagg
    46381 aattgctcgt caattggagc aacgtgttgc tttccgtcgt gcacaaaaac aagcaatcca
    46441 acgtgcaatg cgtgctggag ctaaaggaat caaaactcaa gtatcaggtc gtttgaacgg
    46501 tgcagatatc gcccgtgctg aaggatactc tgaaggaact gttccgcttc acacacttcg
    46561 tgcagatatc gattacgctt gggaagaagc agatactaca tacggtaaac ttggtgttaa
    46621 agtatggatc taccgtggtg aagttcttcc agctcgtaaa aacactaaag gaggtaaata
    46681 accaatgtta gtacctaaac gtgttaaaca ccgtcgtgag ttccgtggaa aaatgcgcgg
    46741 tgaagcaaaa ggtggaaaag aagtagcatt cggtgaatac ggtcttcaag ctacaactag
    46801 ccactggatc actaaccgcc aaatcgaagc tgctcgtatc gccatgactc gttacatgaa
    46861 acgtggtggt aaagtttgga ttaaaatctt cccacacaaa tcatacactg ctaaagctat
    46921 cggtgtgcgt atgggatctg gtaaaggggc acctgaaggt tgggtagcac cagttaaacg
    46981 tggtaaagtg atgttcgaaa tcgctggtgt atctgaagag attgcacgtg aagcgcttcg
    47041 acttgctagc cacaaattgc cagttaaatg taaattcgta aaacgtgaag cagaataagg
    47101 agaaggcatg aaacttaatg aagtaaaaga atttgttaaa gaacttcgtg gtctttctca
    47161 agaagaactc gcgaagcgcg aaaacgaatt gaaaaaagaa ttgtttgaac ttcgtttcca
    47221 agctgctact ggtcaattgg aacaaacagc tcgcttgaaa gaagttaaaa aacaaatcgc
    47281 tcgtatcaaa acagttcaat ctgaagcgaa ataatagact agggaaggag aaatttcaat
    47341 ggaacgcaat aatcgtaaag ttcttgttgg acgtgttgta tctgacaaaa tggacaagac
    47401 aatcacagtt gtagttgaaa caaaacgtaa ccacccagtc tatggtaaac gtattaacta
    47461 ctctaaaaaa tacaaagctc atgatgaaaa caatgttgcc aaagaaggcg atatcgtacg
    47521 tatcatggaa actcgcccgc tttcagctac aaaacgtttc cgtcttgtag aagttgttga
    47581 agaagcggtc atcatctaat caaacctgaa aggagaaaac tgaaatgatt caaacagaaa
    47641 ctcgtttgaa agtcgcagac aacagcggtg ctcgcgaaat cttgactatc aaagttcttg
    47701 gtggttcagg acgtaaattt gcaaacatcg gtgatgttat cgtggcatct gtaaaacaag
    47761 ctactcctgg tggtgcggtt aaaaaaggtg acgttgttaa agcagttatc gttcgtacta
    47821 aatcaggtgc tcgtcgtgct gatggttcat acatcaaatt tgacgaaaac gcagcagtta
    47881 tcatccgtga agacaaaact cctcgcggaa cacgtatctt tggcccagtt gcacgtgaat
    47941 tgcgtgaagg tggcttcatg aagatcgtgt cacttgctcc agaagtactt taatttttag
    48001 gaacaaacta gtcccctagc ttcaagctag ggtgccctta tgggcgtaag aaaaatcaag
    48061 gagaaaccta atgtttgtaa aaaaaggcga caaagttcgc gtaatcgctg gtaaagataa
    48121 gggaacagaa gctgttgtcc ttactgccct tccaaaagta aacaaagtta tcgttgaagg
    48181 tgttaacatt gttaagaaac accaacgtcc aactaacgag cttcctcaag gtggtatcat
    48241 cgagaaagaa gcagctatcc acgtatcaaa tgttcaagtt ttggacaaaa atggtgtagc
    48301 tggtcgtgtt ggttacaaat ttgtagacgg taaaaaagtt cgctacaaca aaaaatcagg
    48361 cgaagtgctt gattaatcac gaaggaaagg agaagtataa tggcaaatcg tttaaaagaa
    48421 aaatatctta atgaagtagt tcctgctttg acagaacaat tcaactactc atcagtgatg
    48481 gctgtgccta aagtagataa gattgttttg aacatgggtg ttggtgaagc tgtatcaaac
    48541 gctaaaagcc ttgaaaaagc tgctgaagaa ttggcactta tctcaggtca aaaaccactt
    48601 atcactaaag ctaaaaaatc aatcgccggc ttccgtcttc gtgaaggtgt tgcgatcggt
    48661 gcaaaagtta cccttcgtgg tgaacgtatg tacgaattct tggataaatt ggtatcagtt
    48721 tcacttccac gtgtacgtga ctttcacggt gttccaacaa aatcatttga tggacgcggg
    48781 aactacacac ttggtgtgaa agaacaatta atcttcccag aaatcaactt cgatgacgtt
    48841 gacaaaactc gtggtcttga catcgttatc gtaacaactg ctaacactga cgaagagtca
    48901 cgtgcattgc ttacaggcct tgggatgcct tttgcaaaat aatataggag gtaaatctaa
    48961 tggctaaaaa atcaatggta gctagagagg ctaaacgcca aaaaattgtt gaccgttatg
    49021 ctgaaaaacg tgctgcatta aaggcggcag gggactacga aggtttatct aaattacctc
    49081 gcaacgcctc accgactcgt ttacataatc gttgtagagt tacggggcgc ccacattcag
    49141 tttaccgcaa atttggtctg agtcgtatcg cttttcgcga acttgcgcat aaaggtcaaa
    49201 ttcctggtgt aacaaaagca tcttggtaat ttaagatatc aagggtgtca aaactccaag
    49261 taaaaatagg aaacttgacg aagaaactaa agtttctagg aaagtttatc tttttcacac
    49321 agagtttagc ccgggttcaa ttgggcttgc caatttgaac ccgagctaca gctttggcaa
    49381 aaaaagacca atttgctttg gagcattgct tctgcattaa attgtctatt tttgctcgtg
    49441 ctgttacgct ctttgtatca tgtattaact agcaagtgca acttgcaaac tactagtaag
    49501 aggagaaaaa caaaatggtt atgactgacc caatcgcaga cttcctaact cgtattcgta
    49561 atgctaacca agctaaacac gaagtacttg aagtacctgc atcaaacatc aaaaaaggga
    49621 ttgctgaaat ccttaaacgc gaaggttttg taaaaaacgt tgaaatcatt gaagatgaca
    49681 aacaaggcgt catccgtgta tttcttaaat acggaccaaa tggtgaaaaa gttatcacta
    49741 acttgaaacg tgtttctaaa ccaggacttc gtgtctacaa aaaacgtgaa gaccttccaa
    49801 aagttcttaa cggacttgga attgccatcc tttcaacttc tgaaggtttg cttactgata
    49861 aagaagcacg ccaaaagaat gttggtggtg aggttatcgc ttacgtttgg taaaatcaag
    49921 atacaaagct cgtaaagaac aaagcaaaat taggaagttg gagaagtttg tttacaaaca
    49981 agccaactta tctattttgc acagttctta gagcgtgttc agttaagctc ttgaactaaa
    50041 taagtatctg aaccccgtga aaactggccg ttctggcctg acaatttaac aggagaaaat
    50101 aaacatgtca cgtattggta ataaagttat cgtgttgcct gctggtgttg aactcactaa
    50161 caatgacaac gttgtaactg taaaaggacc taaaggagaa cttactcgtg agttctcaaa
    50221 agatattgaa atccgtgtgg aaggtactga agtaactctt caccgtccaa acgattcaaa
    50281 agaaatgaaa actatccacg gaactactcg tgcccttttg aacaacatgg ttgttggtgt
    50341 atcagaagga ttcaagaaag aacttgaaat gcgtggggtt ggttaccgtg cacagcttca
    50401 aggatctaaa cttgttttgg ctgttggtaa atctcatcca gacgaagttg aagctccaga
    50461 aggaattact tttgaacttc caaacccaac aacaatcgtt gttagcggaa tttcaaaaga
    50521 agtagttggt caaacagctg cttacgtacg tagccttcgt tcaccagaac catataaagg
    50581 taaaggtatc cgttacgttg gtgaattcgt tcgccgtaaa gaaggtaaaa caggtaaata
    50641 atgttgagtg gttgatcatc aaccaccaac ctattttcca actttgtgca tagcacacga
    50701 tttaaaacta aagaggtgaa aactgtgatt tcaaaaccag ataaaaacaa actccgccaa
    50761 aaacgccacc gtcgcgttcg cggaaaactc tctggaactg ctgatcgccc acgtttgaac
    50821 gtattccgtt ctaatacagg catctacgct caagtgattg atgacgtagc gggtgtaacg
    50881 ctcgcaagtg cttcaactct tgataaagaa gtttcaaaag gaactaaaac tgaacaagcc
    50941 gttgctgtcg gtaaactcgt tgcagaacgt gcaaacgcta aaggtatttc agaagtggtg
    51001 ttcgaccgcg gtggatatct atatcacgga cgtgtgaaag ctttggctga tgcagctcgt
    51061 gaaaacggat tgaaattcta ataggaggac actagaaaat ggcatttaaa gacaatgcag
    51121 ttgaattaga agaacgcgta gttgctgtca accgtgttac aaaagttgtt aaaggtggac
    51181 gtcgtcttcg tttcgcagct cttgttgttg ttggtgacca caatggtcgc gtaggatttg
    51241 gtactggtaa agctcaagaa gttccagaag caatccgtaa agcagtagat gatgctaaga
    51301 aaaacttgat cgaagttcct atggttggaa caacaatccc acacgaagtt ctttcagaat
    51361 tcggtggagc taaagtattg ttgaaacctg ctgtagaagg ttctggagtt gccgctggtg
    51421 gtgcagttcg tgccgttgtg gaattggcag gtgtggcaga tattacatct aaatcacttg
    51481 gttctaacac tccaatcaac attgttcgtg caactgttga aggtttgaaa caattgaaac
    51541 gcgctgaaga aattgctgcc cttcgtggta tttcagtttc tgatttggca taagaaaggg
    51601 gataaaatgg ctcaaattaa aattactttg actaagtctc caatcggacg cattccatca
    51661 caacgtaaaa ctgttgtagc acttggactt ggcaaattga acagctttgt tattaaagaa
    51721 gataacgctg ctatccgtgg tatgatcaca gcagtatctc acttagtaac agttgaagaa
    51781 gtaaactaat gaagttttag gggatgtgca ctgtaccatc ccctaaaact agatatagtc
    51841 atctatgatg acatcgtata ggcgagttga tgggggagac aaccttttct cccttatcgg
    51901 cgctagcatt ttacaaaaga ggagaaaata aaaatgaaac ttcatgaatt gaaacctgca
    51961 gaaggttctc gtaaagtacg taaccgcgtt ggtcgtggta cttcatcagg taacggtaaa
    52021 acatctggtc gtggtcaaaa aggtcaaaaa gctcgtagcg gtggcggagt tcgccttggt
    52081 tttgaaggtg gacaaactcc attgttccgt cgtcttccaa aacgtggatt cactaacatc
    52141 aacgctaaag aatacgcaat tgtgaacctt gaccaattga acgtctttga agatggtgct
    52201 gaagtaactc cagttgttct tatcgaagca ggaattgtta aagctgaaaa gtcaggtatt
    52261 aaaattcttg gtaacggtga gttgactaag aaattgactg tgaaagcagc taaattctct
    52321 aaatcagctg aagaagctat cactgctaaa ggtggttcag tagaagtcat ctaagagagg
    52381 tgacctatgt tttttaaatt attaagagaa gctcttaaag tcaagcaggt tcgatcaaaa
    52441 attttattta caatttttat cgttttggtc tttcgtatcg gaactagcat tacagttcct
    52501 ggtgtgaatg ccaatagctt gaatgcttta agtggattat ccttcttaaa catgttgagc
    52561 ttggtgtcgg ggaatgccct aaaaaacttt tcgatttttg ccctaggagt tagtccctat
    52621 atcaccgctt ctattgttgt ccaactcttg caaatggata ttttacccaa gtttgtagag
    52681 tggggtaaac aaggggaagt aggtcgaaga aaattgaatc aagctactcg ttatattgct
    52741 ctagttctcg cttttgtgca atctatcggg attacagctg gttttaatac cttggctgga
    52801 gctcaattga ttaaaactgc tttaactcca caagtttttc tgacgattgg tatcatctta
    52861 acagctggta gtatgattgt cacttggttg ggtgagcaaa ttacagataa gggatacgga
    52921 aacggtgttt ccatgattat ctttgccggg attgtttcct caattccaga gatgattcag
    52981 ggcatctatg tggactactt tgtgaacgtc ccaagtagcc gtatcacttc atctatcatt
    53041 ttcgtaatca ttttgattat tactgtattg ttgattattt actttacaac ttatgttcaa
    53101 caagcagaat acaaaattcc aatccaatat actaaggttg cacaaggtgc tccatctagc
    53161 tcttaccttc cgttaaaggt aaatcctgct ggagttatcc ctgttatctt tgccagttcg
    53221 attactgcag cgcctgcggc tattcttcag tttttgagtg ccacaggtca tgattgggct
    53281 tgggtaaggg tagcacaaga gatgttggca actacttctc caactggtat tgccatgtat
    53341 gctttgttga ttattctctt tacattcttc tatacgtttg tacagattaa tcctgaaaaa
    53401 gcagcagaga gcctacaaaa gagtggtgcc tatatccatg gagttcgtcc tggtaaaggt
    53461 acagaagaat atatgtctaa acttcttcgt cgtcttgcaa ctgttggttc cctcttcctt
    53521 ggtgtgattt ccattttacc gattgcagct aaagatgtat ttggtctttc tgatgttgtt
    53581 gcctttggtg gaacaagtct cttgatcatt atctctacag gtatcgaagg aatcaagcaa
    53641 ttggaaggtt acctattgaa acgtaagtat gttggtttca tggacagaac agaataaaag
    53701 tatttactga atcagtaaat gctgagggag tggaggttta aactctgaca tttgtaagag
    53761 ttggatctcc cctcttctat tttgttttta aataggggtg aaaagacttt ttgcttctat
    53821 ttaaaaataa aataaggaga tcaaatcatg aatcttttga ttatgggctt acctggtgca
    53881 ggtaagggaa ctcaagcagc aaaaatcgta gaacaattcc atgttgcaca tatctcaaca
    53941 ggtgatatgt tccgcgctgc aatggcaaat caaactgaaa tgggtgttct tgctaagtca
    54001 tatattgaca agggtgaatt ggttcctgac gaagttacaa atggaatcgt aaaagaacgt
    54061 ctttcacaag atgatattaa agaaacagga ttcttattgg atggttaccc acgtacaatt
    54121 gaacaagctc aggccttgga caaaacattg gctgaacttg gcattgaact agaaggtgtt
    54181 atcaatattg aagtgaaccc taacagcctt ttggaacgtt tgagtggccg tatcatccac
    54241 cgcgtaactg gagaaacttt ccacaaggtc tttaacccac cagttgacta taaagaagaa
    54301 gattactacc aacgtgaaga tgataagcct gagacagtaa aacgtcgttt ggatgttaat
    54361 attgctcaag gagaaccaat cattgctcac taccgtgcca aaggtttggt tcatgacatc
    54421 gaaggtaatc aagatatcaa tgatgtcttc tcagatattg aaaaagtatt gacaaatttg
    54481 aaataaagcg tttttcacac ttgccaaaat ccgctacaaa tgttatactg agatagtctg
    54541 acttataatt gttgtctctg tgtctagagg catcgaatcg aaatttatgg aggtgctttt
    54601 gcgtggcaaa agacgatgtg attgaagttg aaggcaaagt agttgataca atgccgaatg
    54661 caatgtttac ggttgaactt gaaaatggac atcagatttt agcaacagtt tctggtaaaa
    54721 ttcgtaaaaa ctatattcgt attttagcgg gagatcgtgt tactgtcgaa atgagtccat
    54781 atgacttgac acgtggacgt atcacttacc gctttaaata atcgaaaaac ttggagggat
    54841 aagaaatgaa agtaagacca tcggtcaaac caatttgcga atactgtaaa gttattcgtc
    54901 gtaatggtcg tgttatggta atttgcccag caaatccaaa acacaaacaa cgtcaaggat
    54961 aagatagaaa ggagaaaaca tggctcgtat tgctggagtt gatattccaa atgacaaacg
    55021 cgtagtaatc tcattgactt atgtttatgg tatcggactt gcaacatcta agaaaatttt
    55081 ggctgctgct ggaatctcag aagatgttcg tgtacgtgat cttacatcag atcaagaaga
    55141 tgctatccgt cgtgaagtgg atgcaatcaa agttgaaggt gaccttcgtc gtgaagtaaa
    55201 cttgaacatc aaacgtttga tggaaatcgg ttcataccgt ggtatccgtc accgtcgtgg
    55261 acttcctgtc cgtggacaaa acactaaaaa caacgctcgc actcgtaaag gtaaagctgt
    55321 tgcgattgct ggtaagaaaa aataatatag gaggtaaaag tcttggctaa accaacacgt
    55381 aaacgtcgtg tgaaaaagaa tatcgaatct ggtattgctc atattcacgc tacatttaat
    55441 aacactattg ttatgattac tgatgtgcat ggtaatgcaa ttgcttggtc atcagctggt
    55501 gctcttggtt tcaaaggttc tcgtaaatct acaccattcg ctgctcaaat ggcttctgaa
    55561 gctgctgcta aatctgcaca agaacacggt cttaaatcag ttgaagttac tgtaaaaggt
    55621 ccaggttctg gtcgtgagtc agctattcgt gcgcttgctg ccgctggtct tgaagtaaca
    55681 gcaattcgtg atgtgactcc agtgccacac aatggtgctc gtcctccaaa acgtcgccgt
    55741 gtataatcat cgcattacac tgcttttcgt ttaagaggga gtaactaaat gatcgagttt
    55801 gaaaaaccaa atataacaaa aattgatgaa aataaagatt atggcaagtt tgtaatcgaa
    55861 ccacttgaac gtggctacgg tacaactctt ggtaactctc ttcgtcgtgt acttctagct
    55921 tctctaccgg gagcagctgt gacatctatc aacattgatg gtgtgttaca tgagtttgac
    55981 acagttccag gtgttcgtga agacgtgatg caaatcattc tgaacattaa aggaattgca
    56041 gtgaaatcgt acgttgaaga cgaaaaaatc atcgaactgg atgttgaagg tcctgctgaa
    56101 gtaacagctg gtgacatttt gacagatagc gatattgaaa ttgtaaatcc agatcattat
    56161 ctctttacaa tcggtgaagg ttcttctcta aaagcgacta tgactgttaa cagtggtcgt
    56221 ggatatgtac ctgctgatga aaataaaaag gataatgcac cagttggaac acttgctgta
    56281 gattctattt atacaccagt tacaaaagtc aactatcaag tggaacctgc tcgtgtaggt
    56341 agcaatgatg gtttcgacaa attaaccctt gaaatcttga caaatggaac aattattcca
    56401 gaagatgctt tagggctttc agcacgtatt ttgacagaac atcttgattt gtttacaaat
    56461 cttactgaga ttgctaagtc aactgaagtg atgaaagaag ctgatactga atctgacgac
    56521 cgtattttag atcgtacgat tgaggaactg gacttgtctg tgcgttcata caactgttta
    56581 aaacgtgccg gtatcaatac tgtgcatgat ttgacagaaa aatctgaagc agagatgatg
    56641 aaagtacgaa atcttggacg caagagtttg gaagaagtga aactcaaact cattgatttg
    56701 ggtcttggat taaaagataa ataaaggagg aatacatggc ttaccgtaaa ctaggacgca
    56761 ctagctcaca acgtaaagca atgcttcgcg atttgacaac tgaccttttg atcaacgaat
    56821 caatcgtgac aactgaagct cgtgctaaag aaatccgtaa aactgttgaa aaaatgatta
    56881 ctctaggtaa acgtggtgat ttgcatgcac gtcgtcaagc agctgctttc gtacgtaatg
    56941 aaatcgcatc tgaaaactat gatgaagcaa ctgataagta cacttctact acagcacttc
    57001 aaaaattgtt ctcagaaatc gcacctcgtt atgctgaacg taacggtgga tacactcgta
    57061 tccttaaaac tgaaccacgt cgtggtgatg cagcgccaat ggcgatcatc gaattagtat
    57121 aaaatcatca attttgttga gtgttatgat gatggagtct tgtgctctta gtctagctct
    57181 ggtctaccgc taggatttcg gtcctagcgg gaacactcat cataagttgg gatagtagac
    57241 gcttgtttac gaaattgttt ttttcttaag aacaacttcg taagcaggcg tttttgagta
    57301 ttttcgttag aattatgcta tactatttga aaagaatcct gtttaatgtt aaggtttctt
    57361 attttaagaa gaattggagt ttgcttatga aagcgattat aactgttgtt ggtaaagata
    57421 aatctggaat tgttgcaggt gtttctggta aaattgcaga attaggattg aatattgacg
    57481 atatctctca aactgtcttg gatgaatatt ttacgatgat ggctattgta tctagtgatg
    57541 aaaagcaaga ttttacctat cttcgtaatg aatttgaagc ttttgggcaa gctttgaatg
    57601 taaaaatcaa tattcagagt gcagcgattt tcgaagctat gtataatatc taggaggtta
    57661 tcatggatat tagacaagtt actgaaacca tcgccatgat tgaggagcaa aatttcgata
    57721 tcagaaccat taccatgggg atttctcttt tggactgtat cgatccagat atcaatcgtg
    57781 ctgcggagaa aatctatcaa aaaattacga caaaggcggc taatttagta gctgttggtg
    57841 atgaaattgc ggctgagttg ggaattccta tcgttaataa gcgtgtatcg gtgacaccta
    57901 tttctctgat tggggcagcg acagatgcga cggactacgt ggttctggca aaagcgcttg
    57961 ataaggctgc gaaagagatt ggtgtggact ttattggtgg tttttctgcc ttagtacaaa
    58021 aaggttatca aaagggagat gagattctca tcaattccat tcctcgcgct ttggctgaga
    58081 cggataaggt ctgctcgtca gtcaatatcg gctcaaccaa gtctggtatt aatatgacgg
    58141 ctgtggcaga tatgggacga attatcaagg aaacagcaaa tctttcagat atgggagtgg
    58201 ccaagttggt tgtattcgct aatgctgttg aggacaatcc atttatggcg ggtgcctttc
    58261 atggtgttgg ggaagcagat gttatcatca atgtcggagt ttctggtcct ggtgttgtga
    58321 aacgtgcttt ggaaaaagtt cgtggacaga gctttgatgt agtagccgaa acagttaaga
    58381 aaactgcctt taaaatcact cgtatcggtc aattggttgg tcaaatggcc agtgagagac
    58441 tgggtgtgga gtttggtatt gtggacttga gtttggcacc aacccctgcg gttggagact
    58501 ctgtggcacg tgtccttgag gaaatggggc tagaaacagt tggcacgcat ggaacgacgg
    58561 ctgccttggc cctcttgaac gaccaagtta aaaagggtgg agtgatggcc tgcaaccaag
    58621 tcggtggttt atctggtgcc tttatccctg tttctgagga tgaaggaatg attgatgcag
    58681 tgcaaaatgg ctctcttaat ttagaaaaac tagaagctat gacggctatc tgttctgttg
    58741 gattggatat gattgccatc ccagaagata cgcctgctga aaccattgcg gctatgattg
    58801 cggatgaagc agcaatcggt gttatcaaca tgaaaacaac agctgttcgt atcattccca
    58861 aaggaagaga aggcgatatg attgagtttg gtggtctatt aggaactgca cccgttatga
    58921 aggttaatgg ggcttcgtct gtcgacttca tctctcgcgg tggacaaatc ccagcaccaa
    58981 ttcatagttt taaaaattaa gaaaatagga gaaattttaa gttctattta agattagacg
    59041 tgtatactat aatcattaaa taaagacctc ctaatattat ttgaaacaga taacactgaa
    59101 ttagtttgaa tttgattttc atctaatatc tttatttaat gaactcctaa actttttcat
    59161 aataatctcc ttcaaaagtc gcctgtatgg gtggctttta ttttatcatt catgatataa
    59221 tagaagcaaa cggaggacgg aaaatggtaa aagtacgatt gtatttggta cgtcatggca
    59281 agaccatgtt taacacgatt ggtcgcgcgc aaggttggag cgatactccc ttaactgctg
    59341 aaggtgaacg agggattcaa gagttaggaa tcggtttgcg agaatctgat ctacagtttg
    59401 agcgcgctta ttcgagtgat tctggtcgta ccattcagac catgggaatt atccttgaag
    59461 aacttggctt gcaggggaaa atcccttatc gcatggacaa gcgtatcaga gaatggtgtt
    59521 tcggtagttt tgatggagcc tatgatggcg atcttttcat gggcattatt cctcgtatct
    59581 ttaatgtgga ccacgttcac caattgtctt atgctgaact ggctgagggc ttggtagagg
    59641 tcgatacagc tggttgggct gaaggctggg aaaaactcag tggccgaatc aaggaaggct
    59701 ttgaaatgat tgcaaaagaa atggaagatc aaggtggagg taacgccctt gttgtcagcc
    59761 atggaatgac tattggaacc attgtttatc tgattaatgg catgcatccg catggtctag
    59821 ataatggtag cgtgacgatt cttgaatatg aggacggtca gtttagggtt gaagttgtcg
    59881 gtgaccgtag ttaccgagag ctaggacgtg agaagatgga agaaggctct atttaatcag
    59941 tctagacttg cttgccatga gctagggatt tgataagaat atcaagataa gaaaaaacag
    60001 ccgagggtac tcctttcggc tgtttttgat gtggaaaact aaagtgtaat gctattgctt
    60061 ttagagattt tcataaacaa gagcaaggaa cctactgtta gaacagtcag gatagttgac
    60121 aaggctgcgg ctacaccgta atttcctctg agaacctctg tataaatagc tacagtcatt
    60181 gttcttgttt tgacattgta gaggaggata gaagtagaga gttttgaaat cattgtgacc
    60241 caagataaga tggctccaga aatgatacca gatagcatca ttggagttgt aatcttagca
    60301 aaggtattga gacgactact tcctaagctt tcagcagctt cttcaatact tggtgctatt
    60361 tgttgtaagc tagcaacaga tgagcgaata gtataaggta atcttctgac agatagagac
    60421 ataatcaaga tgaaagcagt ccctgtaatc ataagaaatc cacttccaaa tagaccagta
    60481 ttgaaggaag aaatgaaggc aatccctaga acggttcctg gtacaatata aggtaccata
    60541 ctgaggctgt caattaagtt tgtaaacaaa ttccgttttc taacggctag gtaggagata
    60601 aatgtcgcaa atagaacaac tagaactaag gcaatcaaag ggatacgaat ggtattgaaa
    60661 atagcagatc ccatacgatg gaaagctacc ttgtaactgt ttggagaata acctttaaca
    60721 gataccatac ctgatgtttt taggaaagag gtataaatta agtagatttg aggtaaaaca
    60781 gagataaaga taattccgta gactgttgca taaatggcag ctatttttcc ttttgtagtt
    60841 tttttaggct caattggatg gagcagattc atgctgaaac tgtagcggtt tgcaatgtgt
    60901 ttttggataa ggaaaattgc caaggcaatg ataatcgcca taattgcaaa agcagaattt
    60961 cctccaacct cgctaataaa ttgggtataa atcaggacag ggaaagtccg atatccttcg
    61021 ccaatcaaca taggcgttcc aaagtctgag aatgctctca taaatacaag caaggagctg
    61081 ctagtaaggt tggaactagg agaggtaaaa caaccgttac gataggttta aatccgaagg
    61141 accccatgct ttcagctgct tcaagtagag aattgtcaat actgttcatt gttccagcaa
    61201 catatagaaa taccagtggg aatagttgca gtgtaaagac aagtacaatt cctttgaatc
    61261 cataaatatc gatagctgga agatgaaggg catttgtcaa aaatttagtg atgaccccat
    61321 ttcgtcctag caagagaacc caggagtagg ctcctacgaa aggagctgac atggaagcaa
    61381 tgataatcaa tatttgtaga aatttctccc ccttgaagtc atacatagag aagagataag
    61441 ctaatagggt tcctacaact aaggaagtga cagtagcggt aatggaaacc ttgaaactgt
    61501 tgactagtgt ctcagagtag tagactttac taaagaaagt gacaaaatta gctagtgaga
    61561 attgtccttc atgtataagt gcttacttga gcacgataac gataggataa acgagaaaga
    61621 caagataggt aagaaagatg aagaaagagg aggctgtcca aatatttagt tttttacgtt
    61681 ccatggttga ctccttttat caggttttgg gaaccatctg cagaaaagat gtttaatttt
    61741 tgcgtattga ttcgtagacg aatacgattg cctttttgta gatcttcttc aaaagttgat
    61801 tcttcactaa cttgaatttt tgaggcaaaa cctgtctcaa tgaaatactc cgtatttagt
    61861 ccaagataga cgctatcgct aatagttcct tcaatatctc cagattcatc tttgataaac
    61921 tcttcgggac gaatgcttac atgaatagct tgctcctcaa cctgatcaag agctggcatt
    61981 cgaagggcat agccatctga aaagacgata taagcgccgt cgctccgttt ttcaagattg
    62041 gcagggataa tatttgtgcg tccgataaag gttgccacaa actcattagc tggtttatga
    62101 tagagttctt ttggtcggcc gatttgttgg atcaccccat ctttcataac agcaatttga
    62161 tctgaaatag ccatggcttc ttcttggtcg tgggttacat aaacagttgt aattcccact
    62221 tcgtgttgga tttctcggat ggcttgacgc atatccaagc gaagtttggc ctccagatta
    62281 ctaagtggct cgtccatgag gagaacactt ggattaaccg ctaaggcgca tgccaaggtg
    62341 acacgttgtt gttgtccacc actgagttta tcgggctttc gatccgcata ttgagcaatt
    62401 tgcatgagtt caagatactt gttggtctgt tgaatcaatt cttcttttgg aaccttcttt
    62461 tgcataagac caaaagcaac gttgtctcgg acagtcaaat gtgggaaaat agcgtagttt
    62521 tggaaaacca tcccgatatt gcgtttgctg ggttccatat tattgatttt tgtatcatcg
    62581 aagtaaaatt ctccatcttt gatactgttg aaacctgcaa tcatacgaag aagggtcgtt
    62641 ttcccacatc ctgaaggtcc aagaagggta aagagacttc cttttggaat tgtaatgttc
    62701 aaattctcaa taacagggac atcgtggtag attttttggc gttaataatt ttgatctcac
    62761 tcatagtgaa ccttttttac tgtttagatt ggatatctgt aaagacttcg ttgtatttct
    62821 taacgatatc tgatttattc ttgatgacat aatcataatc ttcagtgagt gttttgattt
    62881 tgtcaattgg tttcatgttt tcgcttgttt tagcattttt acgaacagga cggttagtag
    62941 tggttgtacc aagtgtatct tgtacttctt gagagataat aaaatcgata aatttcttgg
    63001 cattttccat atttttagat tttttaacga tagcagcact agcaggtagg aagacggttc
    63061 cttcttttgg atagactacc ttaatgttag ctccgtcatt taagagctta actgctggat
    63121 cttcataaga gagaccaaca gtcatttctc catcagcgac tgctttatag acaccagatg
    63181 aacttaaacc gattttacca tcaataagtg tgaaaagatc ttttacataa gaccaagcct
    63241 tatcatcttt gtaaccacct tgagcttgta gcatatttgt taattgagca aaggcgctag
    63301 aagagtttgc tgggtcagca gttgcgattt ttccttttag ttcaggtttg aaaagatcgt
    63361 tatatccttc gatgttcatg cctttagtta aatcagggtt gacgattaaa acactaccat
    63421 ctagtgtata aggagtagag tagccagttg tgttttgata ttctttgata acattatcat
    63481 tttcttttga agtatagttt tcaaagagtt ctccgtgggt agtatattgt gtataagaac
    63541 caccaaagat aacatcagct acaggaactt ctttttctga ctctagtttt ttgaaaagtt
    63601 ctccagtacc agcttgaatc agttctactt tgataccata tttttcttca aaggcaggaa
    63661 tagttgctcc aattaagccc tctgagtttg gtgaataaac gactagcgaa ccgccgtctc
    63721 ctttatcaga tgaactgtca tcggcagatt cattagaaga acaagcagca taatacatcc
    63781 atttcttttt catgatggat acctccgttg tgttatttaa gtttatttta aaacaatgta
    63841 agcgttttta aaacatacaa ttctattcta tagtgtattg aatctataac agtacacttt
    63901 gactgctaaa atatttctat aaattaattt gactttcctg atagagatgt tcacatctta
    63961 tttcaattca ctatattaga gtaaaattct ctacaaaaag aagaatagcc tattttacta
    64021 ttcttccgag tgatttcaat ttctttgggg aaatatggag atacttttta aatactttgg
    64081 caaaatattt atagtctgaa aatccaactt ggtcagagat agcgcttaaa ggggagttgg
    64141 gagattcagc catcttatct atagcttgtt tcaggcgata ttgtaagata tattcattta
    64201 gagtcatggg gaagtcttct ttgataacct tatatagatg cttgttatct gcaaatagct
    64261 agagcatttc aaggaaataa actagaaata acaattaata tggtggttca gaaatacctt
    64321 ttgataatca ataagatctt caggatgaag tgcgttgact ccatccattt catatttccg
    64381 atttaataaa cgatatttgt tttcaccaaa ttgatgaaaa gggagtagtt gaacttggtc
    64441 gatatttaat gagttaaata gagtagcgaa tttttctgca tcctctaaac tattgttaaa
    64501 attaggaata actgggattc ttaaaacgat agttttattt tgagaaaaag cataatgaat
    64561 gtttttaata atcatttgat taaaaacccc agtcactttt ttatgtttta tagaattata
    64621 atgttttagg tctgtgtaga taaaatccac atattgaatt aaatcaataa atttttcatg
    64681 atcaacaaag gcagtagttt caatggcagt gtgtatgtga tgttctttag ctgattttaa
    64741 gatggcttta gcaaattcaa actgagcaaa tatttcacct cctgataaag ttaaacctcc
    64801 accggattct tcgtaaaatt ctttgtcttt taatacctct gtaataattt cttctacagt
    64861 cttttcttca ccgactaagg tgaatttctc tcgttgagca tctttcattt tttcaggttt
    64921 cattctttga gattcaggat tagaacacca tggacagcgc agaggacatc cttttaaaaa
    64981 aacagttgta cgaatacccg ggccgtcatg aattgaaaag tgttgaatat taaaaataat
    65041 tcctttagat atttccataa gagttcctcc tcttcataag tctatcctat cacaaaaacg
    65101 aaagaaaaac aattacgaac gaaagtttaa tttgttgttt tttgcttttt attttggtag
    65161 aataaaacgg aggtgtaaaa tgaaaagatt ggaacaaatt attaaattag tatcagaata
    65221 tgaaaagatc gacgttaata cattatcgga aaaattaaat gtatcgaaag taacgattag
    65281 aaaagattta gataaattag agtcaaaagg tttattacac agagagcatg gatatgctgt
    65341 attaaatagt ggagatgact taaatgtacg tttgtcaatt aattatgaaa ttaagagaaa
    65401 aattgttcag gaagcagtaa aattggtgtc agataatgaa acaataatga tagaatctgg
    65461 atcgacctgt gctttacttg ctgaggaaat ttgcaagcaa aaaagaaatg ttacgattgt
    65521 aacaaattca ttttttatag caaattttgt gagagcttat gattcatgtc gtattattgt
    65581 tcttggtgga gagtttcaga aggattcaca ggttactgtg ggacctttat taaaagaaat
    65641 gatacagact tttcatgtgc gtcaagcttt tgttgggaca gatggctacg ataaagagat
    65701 gggctttaca ggaaaagatt taatgcgcag tgaagtagtt caatatattt cagcagcgtc
    65761 ggataaagtc attgtactaa ctgactcaag taaatttgat aaaaggggta cagtaagaag
    65821 atttgcttta agtcaagtct atgaagtaat aacagacgaa aaactttcta aacaaaatat
    65881 agctacatta gaaaatgctg ggataatggt taaggtagtt tcgtaagagg ttaagtgtat
    65941 gaatcaagat aggaataaac tgctctctaa aattgcttat ctgtattata ttgaaaactt
    66001 aaatcagtca caaatagcag caaaattagg aatttataga acctctatta gtagaatgtt
    66061 aacagaagca aggaatgcag gaattgttaa aattgaaata gagaattttg ataccaatat
    66121 gtttaagttg gaaaattatg taaaagaaaa atatggtctg gaaagtttag aaattattcc
    66181 aaatgaattt gatgataatc caacaatttt atctgaaaga atttctcaag ttgcagtagg
    66241 tgtccttagg aatctaattg atgataatat gaaaattggc ttttcttggg ggaaaagttt
    66301 aagtaattta gtagatttaa ttcacagtaa aagtgtccga aatgttcact tctatcctct
    66361 agcaggtggt cctagtcaca tacacgctaa ataccatgtg aatacactga tttatgaaat
    66421 gtctagaaaa tttcatggag agtgtacatt tatgaatgca acgattgtgc aagaaaataa
    66481 attgttagca gatggtattt tgcaatcaag atattttgaa aatttgaaaa atagttggaa
    66541 agatttagat atagctgtag tcggaattgg tgattttagc aataaaggaa agcatcaatg
    66601 gttagacatg cttacagagg atgactttaa agaattgacc aaagttaaaa ctgtaggaga
    66661 aatttgttgt cgattttttg attcaaaagg taaagaagtc tatgaaaact tacaggaaag
    66721 aacgatagca atctctttgg aagatttaaa aaatattcct caaagtttag ctgttgctta
    66781 cggtgatacg aaagtatctt cgattctttc tgtcttgcgt gctaatttag taaatcattt
    66841 gattacagat aaaaatacaa ttttaaaagt tttggaagaa gatggtgatt tgacttttag
    66901 agagattcta ggtgagtgaa aatgatagac tgatcagttt atcgtttttt ctttttagtc
    66961 gattgcacat ttgtgcttat ataaacaaaa atagtttatc tgttgtt
//
LOCUS       DAVKJJ010000003        66538 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000003 DAVKJJ010000000
VERSION     DAVKJJ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 66538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66538
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.003"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(437..2164)
                     /gene="ezrA"
                     /locus_tag="VW144_000158"
     CDS             complement(437..2164)
                     /gene="ezrA"
                     /locus_tag="VW144_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEU9599668.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2246..4192)
                     /gene="gyrB"
                     /locus_tag="VW144_000159"
     CDS             complement(2246..4192)
                     /gene="gyrB"
                     /locus_tag="VW144_000159"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU9599669.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEIVGDTDKTGTTVHFTPDPKIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKREG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4207..4770)
                     /locus_tag="VW144_000160"
     CDS             complement(4207..4770)
                     /locus_tag="VW144_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9599670.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEVISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4855..5409)
                     /locus_tag="VW144_000161"
     CDS             complement(4855..5409)
                     /locus_tag="VW144_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEU9599671.1"
                     /translation="MVKVAVILAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYQDVFGKNQ"
     gene            complement(5420..6643)
                     /locus_tag="VW144_000162"
     CDS             complement(5420..6643)
                     /locus_tag="VW144_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9599672.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6680..9130)
                     /locus_tag="VW144_000163"
     CDS             complement(6680..9130)
                     /locus_tag="VW144_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEU9599673.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SHSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9330..10259)
                     /locus_tag="VW144_000164"
     CDS             complement(9330..10259)
                     /locus_tag="VW144_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9599674.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            10399..10686
                     /locus_tag="VW144_000165"
     CDS             10399..10686
                     /locus_tag="VW144_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEU9599675.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10720..12054)
                     /gene="ciaH"
                     /locus_tag="VW144_000166"
     CDS             complement(10720..12054)
                     /gene="ciaH"
                     /locus_tag="VW144_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEU9599676.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12044..12718)
                     /gene="ciaR"
                     /locus_tag="VW144_000167"
     CDS             complement(12044..12718)
                     /gene="ciaR"
                     /locus_tag="VW144_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEU9599677.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12827..15373)
                     /locus_tag="VW144_000168"
     CDS             complement(12827..15373)
                     /locus_tag="VW144_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU9599678.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGKAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFFAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEARQVASQIFATHKENLAELPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLTAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15496..15936)
                     /locus_tag="VW144_000169"
     CDS             complement(15496..15936)
                     /locus_tag="VW144_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEU9599679.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDIILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTSESKVVLEEFRKVYPL"
     gene            complement(15911..16780)
                     /locus_tag="VW144_000170"
     CDS             complement(15911..16780)
                     /locus_tag="VW144_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEU9599680.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDDDQLKQAITVLRQQGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKIDFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16758..17258)
                     /locus_tag="VW144_000171"
     CDS             complement(16758..17258)
                     /locus_tag="VW144_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9599681.1"
                     /translation="MEIKKHFGVYCVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETRFTVRSYSNPRIYDVFVREELKNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVND
                     EKSTCP"
     gene            complement(17269..17967)
                     /locus_tag="VW144_000172"
     CDS             complement(17269..17967)
                     /locus_tag="VW144_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEU9599682.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(17960..18196)
                     /locus_tag="VW144_000173"
     CDS             complement(17960..18196)
                     /locus_tag="VW144_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEU9599683.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTIEQNGLALEVT
                     PYFLINVSGEGEGFSMWSPTACDVLATDWVEVHD"
     gene            complement(18296..19222)
                     /locus_tag="VW144_000174"
     CDS             complement(18296..19222)
                     /locus_tag="VW144_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEU9599684.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCKLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19297..20112)
                     /locus_tag="VW144_000175"
     CDS             complement(19297..20112)
                     /locus_tag="VW144_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9599685.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20116..20436)
                     /locus_tag="VW144_000176"
     CDS             complement(20116..20436)
                     /locus_tag="VW144_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9599686.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(20733..20813)
                     /locus_tag="VW144_000177"
                     /pseudo
     CDS             complement(20733..20813)
                     /locus_tag="VW144_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(20857..22854)
                     /gene="metG"
                     /locus_tag="VW144_000178"
     CDS             complement(20857..22854)
                     /gene="metG"
                     /locus_tag="VW144_000178"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU9599687.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFCLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(22978..24237)
                     /locus_tag="VW144_000179"
     CDS             complement(22978..24237)
                     /locus_tag="VW144_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9599688.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIANIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(24239..24940)
                     /locus_tag="VW144_000180"
     CDS             complement(24239..24940)
                     /locus_tag="VW144_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9599689.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(24924..26123)
                     /locus_tag="VW144_000181"
     CDS             complement(24924..26123)
                     /locus_tag="VW144_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU9599690.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVANEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAQAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            26409..27755
                     /gene="gor"
                     /locus_tag="VW144_000182"
     CDS             26409..27755
                     /gene="gor"
                     /locus_tag="VW144_000182"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEU9599691.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFNFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESVAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTSSEEFVTMR"
     gene            27951..28487
                     /locus_tag="VW144_000183"
     CDS             27951..28487
                     /locus_tag="VW144_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEU9599692.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQTYFTN"
     gene            28543..28623
                     /locus_tag="VW144_000184"
                     /pseudo
     CDS             28543..28623
                     /locus_tag="VW144_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(28692..28907)
                     /locus_tag="VW144_000185"
     CDS             complement(28692..28907)
                     /locus_tag="VW144_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEU9599693.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            29005..29325
                     /locus_tag="VW144_000186"
     CDS             29005..29325
                     /locus_tag="VW144_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9599694.1"
                     /translation="MRHKFQQVLDKIHDFLNGHDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(29355..29693)
                     /locus_tag="VW144_000187"
     CDS             complement(29355..29693)
                     /locus_tag="VW144_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9599695.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(29705..30424)
                     /gene="trmD"
                     /locus_tag="VW144_000188"
     CDS             complement(29705..30424)
                     /gene="trmD"
                     /locus_tag="VW144_000188"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU9599696.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(30414..30932)
                     /gene="rimM"
                     /locus_tag="VW144_000189"
     CDS             complement(30414..30932)
                     /gene="rimM"
                     /locus_tag="VW144_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU9599697.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(30963..31751)
                     /locus_tag="VW144_000190"
     CDS             complement(30963..31751)
                     /locus_tag="VW144_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9599698.1"
                     /translation="MKRFEVSTEIGSLSVAYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIYDQAGKNLDNLADVVYEALEELGISEYILCVHSWSGI
                     LACKLLEKPIKCQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPIFIFCQT
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            complement(31855..32098)
                     /locus_tag="VW144_000191"
                     /pseudo
     CDS             complement(31855..32098)
                     /locus_tag="VW144_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
     gene            complement(32328..32567)
                     /locus_tag="VW144_000192"
     CDS             complement(32328..32567)
                     /locus_tag="VW144_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU9599699.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(32587..32859)
                     /gene="rpsP"
                     /locus_tag="VW144_000193"
     CDS             complement(32587..32859)
                     /gene="rpsP"
                     /locus_tag="VW144_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU9599700.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLTWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            33029..33238
                     /locus_tag="VW144_000194"
     CDS             33029..33238
                     /locus_tag="VW144_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9599701.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERDLDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            33717..33908
                     /locus_tag="VW144_000195"
     CDS             33717..33908
                     /locus_tag="VW144_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9599702.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDF"
     gene            34022..34825
                     /locus_tag="VW144_000196"
     CDS             34022..34825
                     /locus_tag="VW144_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU9599703.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIGGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(34881..36422)
                     /locus_tag="VW144_000197"
     CDS             complement(34881..36422)
                     /locus_tag="VW144_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9599704.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGIMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHDPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(36424..36906)
                     /locus_tag="VW144_000198"
     CDS             complement(36424..36906)
                     /locus_tag="VW144_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9599705.1"
                     /translation="MVKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKVRN
                     "
     gene            complement(36906..37991)
                     /gene="rlmN"
                     /locus_tag="VW144_000199"
     CDS             complement(36906..37991)
                     /gene="rlmN"
                     /locus_tag="VW144_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU9599706.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMCQHYGLS
                     VCVTTQVGCNIGCTFCSSGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERVSHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSTSGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     LTELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGT
                     DIDAACGQLRSNTMKRDRQKAVAAVNP"
     gene            complement(38012..38542)
                     /locus_tag="VW144_000200"
     CDS             complement(38012..38542)
                     /locus_tag="VW144_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEU9599707.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            complement(38699..39304)
                     /locus_tag="VW144_000201"
     CDS             complement(38699..39304)
                     /locus_tag="VW144_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU9599708.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            complement(39477..40514)
                     /locus_tag="VW144_000202"
     CDS             complement(39477..40514)
                     /locus_tag="VW144_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9599709.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKADSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            complement(40548..41483)
                     /locus_tag="VW144_000203"
     CDS             complement(40548..41483)
                     /locus_tag="VW144_000203"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEU9599710.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            complement(42036..43226)
                     /locus_tag="VW144_000204"
     CDS             complement(42036..43226)
                     /locus_tag="VW144_000204"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU9599711.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            complement(43441..44784)
                     /locus_tag="VW144_000205"
     CDS             complement(43441..44784)
                     /locus_tag="VW144_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9599712.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLEIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            complement(44924..45739)
                     /locus_tag="VW144_000206"
     CDS             complement(44924..45739)
                     /locus_tag="VW144_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9599713.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            complement(45791..47971)
                     /locus_tag="VW144_000207"
     CDS             complement(45791..47971)
                     /locus_tag="VW144_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9599714.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            complement(48257..49183)
                     /locus_tag="VW144_000208"
     CDS             complement(48257..49183)
                     /locus_tag="VW144_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9599715.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANAPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
     gene            complement(49176..49868)
                     /gene="ftsE"
                     /locus_tag="VW144_000209"
     CDS             complement(49176..49868)
                     /gene="ftsE"
                     /locus_tag="VW144_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU9599716.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKKRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            complement(49886..50981)
                     /gene="prfB"
                     /locus_tag="VW144_000210"
     CDS             complement(join(49886..50908,50910..50981))
                     /gene="prfB"
                     /locus_tag="VW144_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU9599717.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            complement(51089..51745)
                     /locus_tag="VW144_000211"
     CDS             complement(51089..51745)
                     /locus_tag="VW144_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9599718.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            complement(52053..52763)
                     /locus_tag="VW144_000212"
     CDS             complement(52053..52763)
                     /locus_tag="VW144_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9599719.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVASGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGRIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            complement(52763..53527)
                     /locus_tag="VW144_000213"
     CDS             complement(52763..53527)
                     /locus_tag="VW144_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9599720.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            complement(53527..54483)
                     /locus_tag="VW144_000214"
     CDS             complement(53527..54483)
                     /locus_tag="VW144_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9599721.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            complement(54487..55356)
                     /locus_tag="VW144_000215"
     CDS             complement(54487..55356)
                     /locus_tag="VW144_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9599722.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLVTAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            complement(55624..56784)
                     /locus_tag="VW144_000216"
     CDS             complement(55624..56784)
                     /locus_tag="VW144_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9599723.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKNNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            complement(56885..57133)
                     /locus_tag="VW144_000217"
     CDS             complement(56885..57133)
                     /locus_tag="VW144_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEU9599724.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            complement(57212..57802)
                     /gene="clpP"
                     /locus_tag="VW144_000218"
     CDS             complement(57212..57802)
                     /gene="clpP"
                     /locus_tag="VW144_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEU9599725.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            complement(57976..58605)
                     /gene="upp"
                     /locus_tag="VW144_000219"
     CDS             complement(57976..58605)
                     /gene="upp"
                     /locus_tag="VW144_000219"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU9599726.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(58694..59161)
                     /locus_tag="VW144_000220"
     CDS             complement(58694..59161)
                     /locus_tag="VW144_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEU9599727.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            complement(59180..59737)
                     /locus_tag="VW144_000221"
     CDS             complement(59180..59737)
                     /locus_tag="VW144_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9599728.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            59862..60707
                     /locus_tag="VW144_000222"
     CDS             59862..60707
                     /locus_tag="VW144_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9599729.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAENIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            60766..61941
                     /locus_tag="VW144_000223"
     CDS             60766..61941
                     /locus_tag="VW144_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9599730.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDNQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEKNREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            61961..62416
                     /locus_tag="VW144_000224"
     CDS             61961..62416
                     /locus_tag="VW144_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9599731.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWPGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(62436..63190)
                     /locus_tag="VW144_000225"
                     /pseudo
     CDS             complement(62436..63190)
                     /locus_tag="VW144_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274794.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            63273..64751
                     /locus_tag="VW144_000226"
     CDS             63273..64751
                     /locus_tag="VW144_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEU9599732.1"
                     /translation="MVFLTDLRKHDRIVKSINQTEGYLTTQVAFSYFEKGDQSLTMSE
                     KSQWGSKLGFILASAGSAIGLGSVWKFPYMTAANGGGGFLLIFLISTILIGFPLLLAE
                     FALGRSAGVSAIKTFGKLGKNNKYNFIGWIGAFALFILLSFYSVIGGWILVYLGIEFG
                     KLFQLGGTGDYAQLFTSIISNPAIALVAQAAFILLNIFIISRGVQKGIERASKVMMPL
                     LFIIFVFIIGRSLSLPNAMEGVLYFLKPDFSKLTSAGLLYALGQSFFALSLGVTVMLT
                     YASYLDKKTNLVQSGISIVAMNISISIMAGLAIFQARSPFNIQSEGGPSLLFIVLPQL
                     FDKMPFGTIFYVLFLFATVTSSVVMLEINVGNITNQDNSKRAKWSVILGILTFVFGIP
                     SALSYGVMADVHIFGKTFFDAMDFLVSNLLMPFGALYLSLFTGYIFKKALAMEELHLD
                     ERAWKQGLFQVWLFLLRFFVPSFQSSSLWSSLPNLCNQKGLE"
     gene            complement(64772..65716)
                     /gene="manA"
                     /locus_tag="VW144_000227"
     CDS             complement(64772..65716)
                     /gene="manA"
                     /locus_tag="VW144_000227"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEU9599733.1"
                     /translation="MSEPLFLQSVMQEKIWGGAKLRDEFGYDIPSEKIGEYWAISAHP
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDDLLTKVPVKAG
                     DFFYVPSGTMHAIGAGIFILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTVKADDLRSTLLVSNDFFAVYKWEITGKVDFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGSHFILPSDVEAWTLEGQGLELIVSHP"
     gene            complement(65848..66129)
                     /locus_tag="VW144_000228"
     CDS             complement(65848..66129)
                     /locus_tag="VW144_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU9599734.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDSVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
BASE COUNT        19577 a        14509 c        12169 g        20283 t
ORIGIN      
        1 aacaaagagg ttttagaggc ctatttaccg tgggactaaa gttgtacaag aaaagtgcaa
       61 ataagaaatc tccagattag gaactatccg tgagttctct agtctggaga tttttcaata
      121 gacttcgtta ttggacggtt accaagttac tcaaagtaca gatttgaagc tgcagataag
      181 actgacgaaa tcactttaat actaaatgaa aatcaaaaag caaactaaga agctagcctc
      241 aggctgtact tgagtacggt aaggcgacgc tgacgtgatt tgaagagatt ttcgaagagt
      301 ataaaacact gttttgaggt ggtggataaa tcggctgggt aaaaactcaa tctcaggaga
      361 aaatgaagta aatctcccca caactataga aaaagatttg attctgctcc tcacacaata
      421 aaatcttttt cttttattaa aaacgaatcg tttcacgtgt tttctcatat gaggtaacaa
      481 agcgattggt tacaccaggc tctgccactt ccaatgcttg agaaatcttg tcaaatgaag
      541 cgtgataatc aaattctttt tcaaaaatat ctaaagcttc gttaaatgct tcttgaatgc
      601 gttcatcaaa tgagcgatag cggttagaat attgcaagag ttgctctgtc aaagttgcat
      661 attgtacaat attataagtt tccgtttcta aagcttccat atcattcgtt gcaatttcaa
      721 gaactcgggt aacagattca atgttaatca ttttttgttc taactcaacc attaaatcct
      781 cggtattatt gcttgccgta aagaataact tcaagaaagt ttgtggaata cctggcagat
      841 tgcgtttttc catgtatcgc ttgatagtat ggagacgatt gacataaaca ttggcctttt
      901 gacgtgcatt aatatcatct ttctcaattt gtgtcaggcg ctcactaact gaaatttgct
      961 catcttcaat atcttttagt tgagtttgta aatcctcaag attttcttca agaactgaat
     1021 aagcttgggt tggttcttct tgattcgaag ttacctcaac aatagctgcc tcaaaactct
     1081 ctaattctgt ctgaatacga cgaacatggc ttgcagctgt ctcaggaagt aaataggtct
     1141 tgttcaaacg tgcaatatct tctcccaata aagtattatt ctctttcata tgttgaaggt
     1201 aagttggaag agttgcaagt agattttcca ctactttctg agcagcaatt tctcgagtaa
     1261 aaatatcata caaggcattg atttcctctt gtgcctgtcc attctcatat tcggcattat
     1321 ccaattccaa ctgacgaata ttctcttggt ttttcttgaa tgcttcataa agcaagtgga
     1381 aacgcgcttc aatatccgtt tcaacaaaat gataattagc atcaattagt ttacgataac
     1441 cggcttccaa atcctgtaat tgatctggca attctgtaga aagcgtcgta accaaggctg
     1501 gaacacgatc cacaatatga cttaaggcca aaatgtgatt ttctgtatta tccaaaatca
     1561 ctgcggcttc cacagggtca cccgatgaat tcaaggttac aaattgtgaa aattcagatt
     1621 ggatattttc taattgtttt tcaatttcat ccaaggcttg accatactgt tctgaatttt
     1681 cagcaactct atgctgaagt tcctcaaata aatccaaagc atgaagaaca cgaccactat
     1741 ttttagattc ttgcttctct aagtctgcca aagcattgcg aattgccgca atatcttctt
     1801 caatcaaagt aatttgactc tcaatttggt caatttgatg actggccttg agaaaacgaa
     1861 atgaatggtt atagccttct gcttcaaaga gattattttc aatatcggca aaagagttga
     1921 gagataaatc gacccatttt tgattccatt cacggaaagc cacttgactt tgtccaatca
     1981 agtgcatatt ttttacagct tctacttcat catttactgg aagattgtat agttcttctt
     2041 ttctttcttc tagcgcctct aatctcccct cgtttcgctt acgtagaaag attgccacta
     2101 cataagccag aactaaaatg actgcaattg caaccattaa ataaattagt tgtccattag
     2161 acatatcaaa ctcctttttt acttgaaaca atcgtaacga ttatatcata ttttttagac
     2221 caagggaact acttctccct attttttaga catcaagtgt actatagaca gcattttctt
     2281 cgataaactc acgacgaggc tctactcgat cccccatcaa catatcaaag attttatctg
     2341 cttctgcagc atcatctaca gaaactctag ccatcaagcg atgttcggga tccatggttg
     2401 tttcccacag ctgatgatcg tccatttcac ctagcccctt ataacgctga atagtcggtt
     2461 tggtacgacc ttcactataa cgggctaaag cttcttggag tttgatttct tgatctgcac
     2521 ccggctggat atattcttta atctcgcttc caaccttgac accatagatt ggtggttggg
     2581 caatataaac ataaccagct tctaggattg gtttcatata acgataaatc aaggttaaaa
     2641 gaagggtacg aatgtgggct ccatcgacat cggcatcggt catcaaaacg agtttttggt
     2701 aacgggcttt cgaaacatca aattctgcgc caaatcctgt tcccatggct gtgaaaagac
     2761 tacgaatttc ttcgttagct agaatcttat ccatacttgc tttttcaacg ttcaaaatct
     2821 taccgcgaat tggaaggata gcctgaaact cacggttacg accagatttg gctgatccac
     2881 cagctgagtc tccttcgacg atgaagagtt ctgtttcagc agggttatta gaagaacagt
     2941 ctgctagttt ccctggaagg ttggaaattt ccaaaccaga ttttttacgt gtgacttcac
     3001 gcgcacgctt ggcagccaca cgagccttgg cagctaaaat ccctttttcc acgatacgtt
     3061 tggcaatctg tggattttcc atgaggaaat cggagaaggc ttcactgaag aggcgattgg
     3121 taatcttgac cacttcgcta tttcccaatt tggtcttggt ttgtccttca aactgtggat
     3181 ttgggtgttt aactgagata actgcagtta agccttcgcg aacatcttcc cctgttaggt
     3241 tgtcttcatt gtctttcagt aacttatttt tacgagcata atcattgata acacgtgtca
     3301 aggctgtacg gaaaccttgt tcatgcgttc caccttcatg tgtatgaata ttattggcga
     3361 aactcatgac attttcatgg taaccagttg tgtactgcat ggctacctca actgtgatat
     3421 catccatctc accgtctgta tagattggtg tatcaaagat tacatccttg ttctcgttga
     3481 tatattcaac gtaactagca atcccacctt cataatgata atgcttggtt tgttccaaac
     3541 cttcacgctt atctgtaatt gaaatttgaa gaccgcgatt tagaaaggcc aactcttgaa
     3601 tccgtttatt taatttatca aaatcaaaga ttgttgtttc agtgaagatt tttgggtccg
     3661 gtgtgaagtg aacagttgtt cctgttttat ccgtatctcc aactatttca agatctgcga
     3721 caacatgacc acgacggtat tcttggtaat gaatcttacc atttttgtga acatgaacgt
     3781 ctaattgagt ggaaagggca ttaactactg acgaccccac cccgtgaaga ccacctgaaa
     3841 ccttgtatcc accaccgccg aactttcctc cagcgtgaag gactgtaaag acggtctcaa
     3901 cagcaggacg gcctgttttt tcctgaatat cgactgggat accacgccca tcatccacaa
     3961 cagtaatcga atcatctggc tcaataaaaa cttgaatatg gctggcaaat cctgccaagg
     4021 cctcgtcaat tgagttatca acaatttccc agactagatg gtgaagacct tcttttgagg
     4081 ttgatccaat gtacatccct ggacgcatac gaacagcctc taagccctct aaaacttgaa
     4141 tttgactggc atcataatcc tgtgcctgca gatttttgat ttcttctgtc atcttattcc
     4201 tttttcttat atgtctaata cttgtcttaa attcacgata ctggtaaaca agtgggtatc
     4261 aagtcctgca gcttgacctg cttcgatatc aatcggccga tcaccaatga caagaccaga
     4321 gctaatctga tacttttctc ttaaataaag catggattcg ggatttggct ttctcttaaa
     4381 gcctgagcta gaagtcacca cttctgtaaa ataagctgct atagaggttt tttctaaaat
     4441 ttccaaaacc tgatcatttc gatgagagac caaaaaatga cggccacctt gatttgaaat
     4501 gacttccaat aggtcagaaa ctccttcaaa taaaatcggg tgttcaagct ctctggcttc
     4561 attttccttg tacttttcta aaaaattctc taaattggga gcgaatgtct caatcgcaaa
     4621 aggagtagaa acctttaaag cttgatagac actgtcatgg tcttgtgtga taccatacag
     4681 tgccaatgtt tcaacaaatg cagctgttga agtttcataa ttatccagta aagttccacc
     4741 taaatcccag atgtaatcgt gatatttcat acccttcatt atagcatttt tttatgaaaa
     4801 aagcgatgat ttccctgagt tttccacata aaaaacgaga gaataactcc cgttttactg
     4861 atttttacca aagacatctt gatagagcat tcctgttcgt aaactctctg cgtcccctcc
     4921 tagttgctcc accaactcgt aggcaaaggc aagggctgtt gaaggacccc gactggttgt
     4981 caactgacca tctaccacta ctgtttcctt gacatagtga ccatcaagga tttgctcttg
     5041 aacgccgtca taacaagtgt atcgcttatt tttcaatatc tctgcttgat tgagggcaat
     5101 tggtgccgca caaatggctg ctagtttctt cccttcttgc tcgaagcttt gcaattcttg
     5161 aatcaaggtc tgattatcac gtaaatgtgc agaaccaggc atacctccag gaagaacaat
     5221 catatcatag tctgataaat ctccatcaaa gacatgatct gctcttactt ggattgcatg
     5281 cgaacccgtt acttgctctt caaaaccaac catatcacat gtgatattgg ctcgacgcaa
     5341 gacatctaca actgtcaagg cttcaatttc ttcaaagccc tgagctaata taactgctac
     5401 ttttaccatg attttctcct tatttaattt gttttaatac aacctttttc cgtttgaatg
     5461 gaacttttcc gctcttttct tcagctagat tagtctggta actcatcgat aaaagcaaac
     5521 caacaccaat cagattactg ataatagctg atcccccttg cgaaatgaaa ggcaagggaa
     5581 tccccgtcaa aggaagtagt ccagtcacag caccgatatt ctcaaagatg tggaagagca
     5641 acatcataat caaacctgtg gaaatataag tgtagaactg gttatttgat ttaagagtaa
     5701 tcttcaacat acggtaaatc aacatgagat agagggcaat aaccaggaca gagccaataa
     5761 agccaaaatc ttctgcaata accgtaaaaa tcatatctga ctctcgaact gggataagca
     5821 gattcgaagc attaaatccc tgaccaaata agccaccact cccaatggca atctgccctt
     5881 gagcctgctg gtaagtcgtt gtttgggcaa actcaaaggg attgagccaa gccaaaatcc
     5941 gattgatttg gtaggtcggc attccaatct ggtgaagaaa agctcgtccg tccttgctaa
     6001 taaagatagc taagaaacca gcaactcctg ttacagcagt cacaaatact gggataataa
     6061 ttttccaaga aacccctgat aataaaacga ttcctgagaa aatggctaca aaaaccaaag
     6121 ccgtccccaa gtcactttga agtgctaaaa gaactaggac tggaatggta aagagaatca
     6181 tccagaaaat taacaaaaag tccagcggaa ccgtgcgtct ccattcctta tgtttctttg
     6241 taaattggac aatgacacga gccaacatga ggatatagga tatcttcata aattctgacg
     6301 gttggaatag ggtaattcca tttattgata cccagttttt ggcacccgtt gatgcaacta
     6361 agcttggatt ataaaataca atcggcaaga tcataagtcc caagcctaaa atatatagaa
     6421 agggggtcac cttccaaaga aattctgtat taaagagcat gaccacaaaa ccaatcacaa
     6481 gccccaaggc aatccaggcg acctgctgcc ctaaaatggg cagaatatta ttggggtaat
     6541 catgactaac ggctatatag atagccacca caccgatgac cagtagaaaa aatactggca
     6601 agagcaaact gtaatcgact cttgagtcga gagaacgttt catatatact aaccttatac
     6661 tttcatacaa tactatttat caaagttcat taaaaaatct atcaatagcc tcgtcaactt
     6721 cggatcgaga gatggtttta acagtcgctt cttctgctag agatgctact atttgtttgc
     6781 cgtatcgttt tccgacgatt ctcctatcca aaataagagt taaggaacgt tggtattcac
     6841 gtctcatact tcttcccaaa gcctgtttta aacgaataat ggccattggc aattgataat
     6901 cataaaaggc atttttccct tcttgattca gttcttgatt aatctttttc gtcaagggtt
     6961 cttgaggatt ttggaaagga agcctcggta caacttgaat cacaaaagga tggcttgaaa
     7021 aatcaactcc ctcccagaaa cttgctgcac caagcaagat ttgttgttca cctttttcaa
     7081 agcgtttctt tagctgatga acatccccat ttttatactg ggccaagtgg ctaactgtaa
     7141 gtaaatccga tactgctaga agcatgtctt tagcggtaaa gagaaccaaa atcggttgct
     7201 ggaaagcttg aatttccact agtaaagcag ctacctctct ggcatagact tctaaggagg
     7261 tttctgttac cagagggaaa tctttgacca tgaccacttc ttgttcctgt tttccccgag
     7321 attcaatctt gacaaattta gcttcaggat agcctaaaag gtctgccaaa gaaaccctct
     7381 gactaatctc aagagtagcc gatactccca ggacttggca actctcagga agtaaagagg
     7441 acagaagagt acggcctgat tttgtagaag aaatctgaat tttcttttga ctcgtttcag
     7501 ttacttcaag ccagtaatca ccttcagctg taaaatagcg aaccagctca tcaaagcctt
     7561 ctacttctaa ttctgaaaaa tactgatgga gattatccag agaatctaag atatttttcc
     7621 tagatttgcc agactgaaat tgttctatca agtagagaca ctcaaagcga atactttcta
     7681 gtatccgttg ttgaaccctg ttttcttctc ctactaaagc cttatcaatt aaatcgataa
     7741 tagattgtat atcgtaggtc tcttgaagca gattttctag agctaacaaa atcttttgga
     7801 cttcatcaat aatcagtaaa cggtcactga caaattcagg attatcttca agtctggtca
     7861 cgagataggc atgattagtc actaaaagct tgcaagtctc tgccctttct tgactacgtt
     7921 tccaaaaatc ttccgtcaca aataagctat gggatgataa attcccatca tgacgaaggt
     7981 ctgctagaaa atgttggtaa cggtagagtt gcccgatttc atccaaatct cctgtctctg
     8041 tctcagtaag ccagaccaag acttgcattt taaagcgtct aaataagcga ttttcatcat
     8101 tttcctgcaa ggaatgataa aaggcatcca acttcagata attttgtggt ccctttaagc
     8161 tatgaatatc tgtatggaac acttccttga gacgtttacc ttcttcttcc atgatttgat
     8221 tttgaagaat ctttgtcgga acactaagaa caatttgtcg ctctttggat tgagataaag
     8281 cgggtaagag atagccatag gttttcccaa tccctgtcgg cgcttgaatc agagagacag
     8341 gttcatcttt caatagcaag ccaacctctt tagcaaaact ttcttgttcc tccctcactt
     8401 caaggttcaa cagagaaata tttttagaaa agtcttgaga tagttttcgt agctccagag
     8461 aagctgccgt tttcttaaaa tacagacctt gaacttggac caagtctgga gaactcagga
     8521 tagattggtt gcgataagtt tcctcaataa ccaggtagga ctcatatagg agagcgtcag
     8581 ccatttccag caagcgttcc aagagacctt taggaagctg ggtcatcttt tcccgtaaaa
     8641 aaagaagtaa ttctgctgta gcttgggcat ctgaaagggc tgtgtgtgcg tgtttaagag
     8701 gaattcctaa ttctcgacac aaaatcggca agctatattt ttccagttca gggaaaaaga
     8761 cctgggccaa ttcgaccgta tcaacacgag ggtttcttag ctcatagcct tcaaaaaata
     8821 aattttccgc caagagatta gcatcaaact gaacattatg ggctacaaaa atcccatcct
     8881 ccaccaagtc aaagattttt ctggcaactt gcgaaaaatc aggtgcttgc gccagacgtt
     8941 ggtctgtcaa tcctgtcagt tctttgatat gagcatccaa gggttcatgc ggattgacat
     9001 ccgtcgtata gtgatcgacg atttctccat cctcaatcac gacaattccc acttggataa
     9061 ttttagcctt actacctgtg ctagttgcct ctaaatccac cacaacatag cgattaccaa
     9121 tcgttttcat tataaaaaat tataccaaaa aaagggccat ttggcacctg caaacatagg
     9181 ataattttca aactcaagaa cactactttt cttagaaatc ccatcgaata gagcattcta
     9241 gcctgctgat ttttttaaaa tagtgaagaa atacgaaaaa ttgagagaag aaatgttttc
     9301 atcttccttc tctcaacgaa tcatgtattt tatttaacca tgtcaggatc gtagtcataa
     9361 aatcctgtat caaggaaacg gtttttaggg tgaagtttac gaacttcttc atccagcaag
     9421 aaggcttggt catggctaaa gttgccctct tgaatcaagc tacgcgcttg gataaagagt
     9481 tttgcaatct caacaaagtt ctgaccagga gtgtagagcc cttctagttt ccagcgagta
     9541 aagccatgct ccaccaattc tgttaatttg atcatcaaat caaggtcatt gttggcaaag
     9601 atatgggtcc catgattatc ttcaaaaatg gaatagtggc tctctggatc acttggctca
     9661 gccaagaaga ggtcacgttt atgcgtcttt tcatcatcga tatgtgtaaa gttatagtag
     9721 ttttgcaaga gtggacgttt agaatgatgg atgacgctag caccgtaaac caaaacttca
     9781 gcaggaattt ccaaaatctc tggcattttg aaaagttcag ctgatggaat ttcacgcgcc
     9841 aaaacagcct cagatgcgcc agccttttgt ccccagaagt taatctgacg actgctagtt
     9901 accatggttg aagcatcgta gatggtctta aatgaataac catcgcggtt aactacgtaa
     9961 aagacgcctg catccccaat cgtaatatag tctgtcttga tttcttccaa gaagtctaag
    10021 aaaggcttga tacggtccat catatcttgg tgcatgagag cattgaccgc aacgagcaat
    10081 tccttaccag catcatgaac caacttagcg atttcacgta attggtcata actaaaggtc
    10141 gttggcagac gaaggccaaa atctttctca ccgacataga tacggtctac gccagcttcg
    10201 agtagttgtt caacttgttc aatactttca gcagttgctg taatgacaat cttttccata
    10261 agaaaaatta taccacattt ctccaaaatc atccatgctt tgaaaatgaa aagggacttt
    10321 attctttttg taacctttct gtaataattt tctgccgaaa aaatgataaa ataggtatgt
    10381 actgttaagg agagaatcat gcccgtaaga aaattacaat cctatgaggt agactatcaa
    10441 gaagaattaa accagcagct tcctcattac caagcttata cacctgaagc gcaatctgat
    10501 gccaatctca aggaaatcct attttttatt aatatcgctg ttttttgtat ctgtattgct
    10561 atctttagtt ttatcttttt agcattaaaa ttatcaactg ctcttgcctt tgccgcagca
    10621 atcggattca gcttacttgt tttaaaagtt caacggtcta ttatcaaacg aaaacgtaga
    10681 agataaatcc aaaatatcgc cccagttgga gcgatatttt tattttttct ttttagatgg
    10741 tgtctgaatg gcaatcttca cttcaaagat tgttcccttg ggtttattat ctttgacagt
    10801 aacagttcct tttagagcat ctacaatttg cttggctagg gataatccta aaccaaaacc
    10861 acctttttgc cgggttctag ctttgtctac tcgataaaaa cggtcaaaaa ttttcttttt
    10921 atcttctgtc gaaataccga ttccattatc agaaacaagt aaataaagat tgcgatcggt
    10981 cgccgagata agaaaatcaa tttcaccatc ctcctcagta tacttgacgg cattatcgaa
    11041 aagaatggtc atcagttgtt tcagaagaag ctgatctgtg acaattgttc gatggatacg
    11101 attttcaaaa cggaagacac gattattttc cgaagcaatc atctcgtagt ttgtgaaagt
    11161 tgtattaaaa aagctagttg gaacttctgc aagctccggc ttaatcccat catctctccg
    11221 agctaagttc agcaagcttg tcgttaaaaa acgcatattt cggacttctt ccaaactcga
    11281 tgcaatgctt tcgctcacat ccataatggt agcttctggc ttacgaaaaa gggtctctaa
    11341 gcgattttgc aaaactgcga gtggagttcg taactcatga ctggcatttt ccacaaaaga
    11401 ctgttgcttc tgcatactct caagcagggg cctaacactg accctagcta gatagagact
    11461 ggcaagtaaa gacaaaatcc agaaactagc catcacgacc acaatcaatt gctcatgctt
    11521 ttgactagcc tgctccaact gactggtatt aatcaagaca gcagcatact tgatattggt
    11581 tgaaaccgaa ctaatattgg tctccatcaa aatcacacga tagatttctt cctgcccata
    11641 gctattaaaa acctgaatct ggtagatatg tcctagttct ttcttttcta acttaatctt
    11701 atccaagccc aaaaatcgat ttccagaaag aagttgagta aagtctttat caaagagaat
    11761 gacttccgta ttggaactga cattaggttt tatttctact ttactagcgt ccgccctagc
    11821 attttctaaa tctttaatct cttctgttgc cctatttata gccagctgaa taactgcttg
    11881 aggattttca ctcagtccat gaagcttatc gtccaccgaa gtataaagac tcgaatgcat
    11941 gacttgtaaa ataatcagag tcattgtaga aaaaatcagg gtgaagacac cgaagttgcg
    12001 gataaaataa ctaaagtcat ccgcatacca tgttttttta agtttactga acatctttta
    12061 aaagataccc aacactacgc aaggtttgca aattctctgc aaaagtggtt ccctttaatt
    12121 tcttacggac ttttgaaaca tagacttcga caaccgaaat cgttgtatca ctatcaaatc
    12181 cccatagacg gtcaaaaatc tgcgtcttag gcaaaatcac attttgattt tgaaggaaat
    12241 aaactagtaa atcgaactct ttccccagca attcgacagg agtatcttca actttaacgg
    12301 tattggttga taaattaacc acgatattcc cataagtcaa ggtgttttca ttaaacttcc
    12361 ctgaacgttt gagaagggcc tgaatccgca ttttaagttc ttctaggtag aaaggtttgg
    12421 tcagataatc atccgctccc agttcaaatc catgtccctt gtcatccaaa ctttcctttg
    12481 cagtcataat cagaactggt gtcgtaattc ccttttcacg caattctttt aagacttgga
    12541 aaccattttt ttctggcaac atcaaatcca gcaaaatcaa gtcatagaca ccactctcag
    12601 cttcgtagag accttcttct ccatcaaata cctgcataac atccgcaaaa tcgtctaaaa
    12661 agtcaaatac tgaatttgac agacctaggt catcctcaac caataagatt tttatcatga
    12721 gaaactcctc cttattaaaa ctattatacc aaatttgcct taaaaaaaac tcaactctct
    12781 gcattttaca tgagatagct gagttttctt tttattttag gcttatttat gcatttccgt
    12841 attgaagaac aactgcttcg actgcagctt tttcacggct aatcaagtca acacgcgctg
    12901 caatttcctt gattcccata ccgatgttac ggctaagagc aaggtcagaa agttgcggtt
    12961 caaagaactc cttgtattcc gccaagcgtt gctgagtctt aaatacatga gcaggaagga
    13021 taacaaagct atcaaagctc atatctcctc caagggctgc cttaatccaa gcccagtttt
    13081 cacgcgccca agaccaagct gttttctgag ttgcttgatg agctaggaat tggtaatacc
    13141 aagcagacaa gtcctgtggt ttgaccacaa atttgtcctt ccaagaagta atcaagtttt
    13201 ggatattatc cgcatctgta ctgtatgcaa gagctgctgt caactggcgt ttaaagacag
    13261 catctgttgc gtgagtataa gtatcaagat aaagtgctaa caagtcttta gtctcatgat
    13321 gtttcatctc attaatcaga acttgtgaac gaatagctgc tgggagttct gcaagattct
    13381 ccttgtgtgt tgcgaagatt tggctagcga cttgacgagc ttctgcatca tttgagcgaa
    13441 tcatcatcga aacagccagc tgacgaacca attcatcctc atctgattct ccgtctttag
    13501 cttcaaaacc aagacggtca tagttatgac gagccaattt agcaaccagt cctttgaagg
    13561 ctgtttcagc atccgttcct tcatcaataa agcgctcaag ggctgaaatc acttgagaaa
    13621 cagctgaaac caccagataa gactcttcct tagcaagttt atcaagaact ggaagcaagt
    13681 ctgcataaga aatgtgccct gcctcagcca acaaacgacg ttcttgaaca atttgcagtt
    13741 tgcttgtgtt atcaagtgtc tctagctcag caagaacagc ttctaacaag tctccttgat
    13801 agtcggtaat atagtgggca gtattttcag tgttgagacg aagagctcct tcattttcag
    13861 caagaagagc tgcgtagcca gggatttcga tactttcagt ttcgagtgta tcaggcaagc
    13921 ctttccagtt gctattgagg ggcaccaccc agagacggtt cttgtcttcg ttctcaccga
    13981 tgaagaattg tttttgtgaa atcttcaaga catcattttc aactttgaca gtaagaactg
    14041 ggtaaccagg ctgttccaac caagaatcca tgaaggctgc gacatcacgt cctgacgctt
    14101 gaccaagggc atcccaaagg tcactaccaa tggtgttgct gtattggtgt ttttcaaagt
    14161 aggcatgcaa acctttagca aaatcagcat ctcctagcca acggcgaagc atgtgcatga
    14221 gacggcttcc tttggcatag acgatagcgc cgtcaaagag tgtattgatt tcatctggat
    14281 gtttaacttc gacgtggaca gactgaacgc catcagtagc gtcacgttca agagcaagag
    14341 gtactccacc tgtttggaaa tcttcaaaga tattccagct tggttcgatg gtatccacac
    14401 agacgtattc catcatatta gcgaaacttt cattgagcca aaggtcatcc caccatttca
    14461 tagtcacgag gttcccaaac cattggtgag ccaattcatg ggccacaaca agggcaactt
    14521 gttgacggct agcaaatgta gagttctcat cgacaaccaa gtaaacttca cggtaggtca
    14581 caagacccca gttttccata gcaccagctg agaagtcagg aagggcgatg tggagagatt
    14641 gaggaattgg atacttaact ccatagtaat cttcgtaaaa ctcgatagag cgaacagcga
    14701 tatccagtga gaaatcaaga tttgaaagtg gatgtgcttt ggttgagtag acacctacca
    14761 gggtaccatt tttagtttta gcggtcaccc cttgcaaatc accagcaaaa aaggccaaca
    14821 agtaagaaga catgcgaggt gttgtctcaa acttccagat acctgtttcc ttacggtttt
    14881 caacatcgat ttctggcatg tttgacaagg ccaattcacc ttctgcttgg tcaaagcgaa
    14941 gagagaggtc aaaagttgct ttggcttcag gctcatccac acatgggaaa gcttcgcgcg
    15001 caaaatggct ctcgaactga gtagacaaga cctccttctt gactccatca actgtataat
    15061 aagaagggta aatccctgtc atgttgtctg taattttacc agaaaaggca agaaccaatt
    15121 caacttgacc agcctcagcc aattcgatat gaagggcttc attgtcatgg tcaactgtaa
    15181 atggacgagc ttgacctgca acttctacag aggtgatttc caagtctttt tggtggaggg
    15241 agatgcggtc actctgtgct ttaccagtga tggtcacttt cccagaaaaa gtcttggtct
    15301 cacgactcaa atctaaaaat aaatcataat gttcaggaac aaattgctta ataaaatgtt
    15361 caactgcttg catagttttc tcctattcta agtttaagag ctgctgctct tcttcaaaca
    15421 aacttattat atcatatttt gcttgaaaaa aaaggattgg acagcgattt ccaaaacttt
    15481 cttccttcta atagtctaca gtgggtagac cttgcgaaat tcttctaaaa caaccttgct
    15541 ttcagatgta aaagtcagcc ctacttcacc caaacagtct gtgaaaaagt cctcatgaac
    15601 ctggcgccaa taggcaagtg atttgtctcc ctcaccttcc ttaaaggcat gttgcgcaga
    15661 aacttgattg aagagttcaa cagaaacctt tgtaatttcg acaatgcaga cagactgatt
    15721 ttgactatct aaaatgatat cgaaggtccc tacttgggga agtggttcgt cttctagtac
    15781 atagaggtca taggctgatg ctgttgctgt cttttctcct ttaaacacca aatccgctaa
    15841 aaggtctggt tcaactccaa aagcccaagc atcgatttca tctccgatca aaggattgat
    15901 ttgcttgtat ttattccaca tttcttgcgg tatcatgggt actcctttgt aattttttac
    15961 tttcttcttt tatgtgttta agatgatctg gatggtcaat ctctaaatca aaaatctctg
    16021 gaatagaact gtagtggata atgcacttga tacccaactg attcattttt tgtatgaaag
    16081 aagtattcag atagcctgct acagcaaaat caatcttgtt ctttcttgct ttatcctgca
    16141 tatctcttag catatctaac attattggac tttccatatc atgccattga ctgtttctca
    16201 tagtcgcaaa aacaaaggaa gtcaaatcat tcattccaac tacaatcttt gaaatgcccg
    16261 tttccagtat actagataag tcaaaatacg ctgacggtaa ttcaatcatc gttccgactt
    16321 tcccagtaaa accctgctga cgcaatactg taatagcttg ttttaattgg tcgtcatcat
    16381 tgacaaaagg aaagataaca gatagattgg ggttggtttg ataaacttct gtaacgacat
    16441 gtgcttcagc ctgaaattca tccaaacacg ccagtaaacg cctggttcct ctatagccaa
    16501 acaagggatg cccttcgtca aaaaactctc tagtccccac taaacaattg gcttctgtat
    16561 tcgttaattc agaaaaacga taccaaactt cttcatctga gtacagggag cagatagtct
    16621 ctagataatc ttttacaaat tgctgacaac ttgctaatat gatgttttga ttaagctctc
    16681 tcaacaaata ttccccacga atcatgccga cgtggtgaaa tagttgagga taaatttttt
    16741 caagaatttt ttcgccacta agggcaagtt gatttctcat cattcacctt ccaattcatg
    16801 taagaagtct tgtccagttc tggaaatcct aataattcag acttaacctt caagactaat
    16861 ggcgatgcat tttcttctgt aatttcttga atatccatcc aaatatatcc aagtgaatca
    16921 ttcgcaccat cagacacagc ttccgaagtc gtaacttgag gtgcactctc attcatttca
    16981 acatcataca aggccatgac atggtgaacc ataaaatttt ttaactcttc tctgacgaaa
    17041 acatcgtaga ttcgaggatt agagtagctt ctaacagtaa atctcgtctc ttccataact
    17101 tctctagtca gcgtttccgt cagtccttca ccaagttgct gactgcctcc aggtagatca
    17161 taccgatgtt gataagggcc tctcgttttt tcaatgcaaa gtaactttcc attttcaaag
    17221 caaacacagt agaccccaaa gtgttttttg atttccatcc aactcctcct acttcaaaga
    17281 ccagccacca tctattgtca agatttgtcc ttgcatggcg ctcgcttttc cacttgctaa
    17341 aaaaagacta agctctgcta tttcctctgg ctcaatccag cgcttgattg gggtttcact
    17401 agccacccag tcagccaaac cacctggttc aaaatccgca gcggtcatag ctgtcttgac
    17461 tgctcctgga gcgataccaa agacctgaat cccagcttca gcatagtcta gagccaactg
    17521 cttggtgaag ccagccaagg catgcttgga tgaagtatag gcgtgaccac ctccacctgc
    17581 taggctagaa gcaatggaac acatattgat gatgattccc tttttatttt ccagcatttg
    17641 tgtcaaataa taccgagtca actctactgg aataatgtag ttgatttcaa aaatctcttg
    17701 aatgtcctgc gccgtttgtt ccaacagtgg tttgtaatca tccaaaactc cggcagtatt
    17761 acacaaaaca tccacctgag ggcaccagtc aaaaataggt tccaagtcca aggtcaaatc
    17821 tctctgtaaa aagtgaaaat caccctctaa gagtggcttt tcaccttggt caactccata
    17881 aacttgatag cccttctcta aaaagaggcg agcttgagcc aatccgatcc ctgaactcac
    17941 tcctgtaatg agtacacgtt tagtcatgca cttctaccca atccgttgcc aaaacatcac
    18001 aagctgtcgg gctccacatg gaaaaacctt ctccttctcc agacacgttg attaggaaat
    18061 aaggtgtcac ttcaagtgca agcccatttt gctcgatggt atcaaagagt tggacatagt
    18121 tttccgcacc tccccaacca gttcgtacat attttttctt agcctttaag ccaggtaaaa
    18181 tttcttcaaa tgtcatgttt ttctccttta atgtcaataa ttctcccctt cattataaca
    18241 aaaaaaccgc tttaaaacgg ctttttgact gtgatttatt caaacctgct actacttacg
    18301 gcaaattatt ccctgcagca agataaattt cataccattc ttttcttgtt aagctaaagt
    18361 ttgccgctcg gctaacttct ctcaagtgct taggatttgt tgtacctacg actgcctgca
    18421 tttttgctgg ataacgcaat atccaagaaa tggcaatagt tgaagaggtt actccatatt
    18481 taatagctaa acgatcaagt acttgattta aagcttgaaa tttctcattt ccaacaaagt
    18541 tccctttaaa atacccgaat tgcaagacag accatgcttg aatgaccaca tcgtgtaatt
    18601 tgcaatattc aaaaatgctg ccatctcgca tagctgcttg actatcttcc atattaacat
    18661 gaaaagctga ttcaaatcct ggagtaaaag ccgcactcaa ttgtagctga ttaacagcta
    18721 acggctgctt gacatctttt ttaagcaact ccatcatcat aggattttga ttagaaactc
    18781 caaaatctcg aactttacct tgtttataaa ggagatcaaa ggcttctgct acttggtcag
    18841 attccatcaa agcatctggt cgatgaagga gcaagctatc tagatgatca atcttcaatc
    18901 tttgcaaaat accgtctact gattttataa tatagtcctt agaaaaatca aaataggtaa
    18961 attcttcaat gcgaatgcca catttggact gaatccacat cttttctctt aaatctggac
    19021 gattttttag gacaagacct aacagttctt cacaacgacc acgaccataa atatcagcca
    19081 agtcgaaggc attgattcca acagaaagtg ctgtttctac aagctcttca acttctttta
    19141 cagacttatc ttttattctc atcattccga gaacaatttc tgataattct ttgtcatctt
    19201 gaccaagagt tatgtatctc atcaaatttt tctcctttaa tttctaacat tcttcccttc
    19261 attataacaa aaaaccgctt tgcaacggct ttttgactat acttcactcc attttatctt
    19321 cttaaaccca cggaacaaga caaagattcc aataaagagg acagctaaag gaataacttt
    19381 tgtaaggaaa acatttgaaa ttcccatcca ctcataataa cggagcagag aacccaccac
    19441 aagatgggca ataatcatac tgacaaatgg acgaaagacc gcttctttcc aattccaaat
    19501 accgataact agcgaaatcg taaagacaga caaactatcc cagggagccg gaatataaaa
    19561 ggctccttct tgtatgaagc ttgccattcc tacatatcct aaaacaacta gaagaactat
    19621 agtcccaaca acaatgtaag tgccaatttt cattttagga gaatcttgga ctaaacttct
    19681 tcgtaaaatt gtggccacaa gtccaaatcc aatcagaaaa ataagaagtt gccctaaaaa
    19741 tgtgagcaaa ttgactgtta agagaggacc tttagaaaaa tcacttagta gttgataata
    19801 acgtaatacc gccaggacaa gaattggcgt caaaagggac tctttgatag aactgcgagg
    19861 tgctcccttg agaatctctt tcattatttt tttaggattc ttacctagat aatcctctgc
    19921 actcatgcca tctcgttctg cttctgagaa atctagcatc atcaaataga tctgctctct
    19981 gagatagtct tcatcataga gaaatccagc aagattaaaa ctttcccaca actcctcaaa
    20041 atacttttga ttctcctcag aaaactcatg tagcaaagcg cttgtttctt cgtaatactt
    20101 cattttcttc atggtttaac ccccattctt aatcccttct actttttgac tcaaatcgtc
    20161 ccattgttgc caaaagactg agacacgctc ttctccttct ttcattaatg aaaaatactt
    20221 ccgatctgga ccatctggcg acgggcgcat gtcgcctctt atccattgat ttttttctaa
    20281 cttttgcaac aaaggataaa tagttcctgg aacgatagta tcaaatccag cctctcgcaa
    20341 agtctgaacc aactcataac cataccgctc tttttgacca atcatatcca agacacaacc
    20401 ttcaagaaca ccttttaata gctgagtttc tttcatcact tctcccttct aatctatttt
    20461 gtaataccta ctagtaactt cacctatagt atatcacttc tacactagtt tgtaaagcat
    20521 aatagttaat actcttcgaa aatctcttca aaccacgtca gcgtcgccct accgtatgta
    20581 tggttactga cttcgtcagt ttcatctaca acctcaaaac agtgttttga gctgacttcg
    20641 tcagtttcat ctacaacctc aaaacagtgt tttgagctga cttcgtcagt ttcatctaca
    20701 acctcaaaaa catgttttga gctgacttcg tcagttctat ctacaacctc aaaacagtgt
    20761 tttgagcaac ctgcggctag cttcctagtt tgctctttga ttttcattga gtataaataa
    20821 aaaaacagaa ctagcctgaa ctagtcctgt ctacttttac ccaatcacac ttccatttgg
    20881 tacagctgga tcaactgtga gaagggttaa tttgccatca tgttcagctg agagaatcat
    20941 accctggctg acatattttt tcatcatttt acgtggtttg aggttagcaa cgatttgaac
    21001 tttcttgccg accaattctt gttcatttgg atagtatttg gcaattcctg aaagaatctg
    21061 acgatcttct ccatcaccag catccaagca gaattgaagc aacttatctg aaccttctac
    21121 tttagacact tctttgactt ctgccacacg gatttcaacc ttgtcaaagt cttcaaactt
    21181 gatttcatcc ttgtttagtt tgagctcaac ttcgtccgga ttccattctt tttcgactgc
    21241 tggtttattg ccttccattt gttccttgat ataggcgatt tcttcttcca tatttagacg
    21301 tggaaagata ggtgttcctt tggcaactac agtcacatct gctgggaagt cagccaaact
    21361 caagttttca agactagaaa cttcttccaa accaagttga gtcaaaactg cacgactagt
    21421 ttccatcata aatggttcaa tcaagtgagc aactacacga atgctggctg ccaagtggct
    21481 catgacactt gccaattggt cacgaagagc ttcatccttg gccaagaccc atggtgcggt
    21541 ctcatcgatg tatttattgg tacgagagat cagagtccag actgcttcaa gcgcacgtgg
    21601 atagtcaact gcttccatgt gtgtatggaa gtctgcgatt gattgttctg caacctcagc
    21661 aagaacatga tcaaattcag tcacaccttc tacataggca gggatttgtc catcaaagta
    21721 cttattaatc atggaaaccg tacggttaag gaggttccca aggtcattag ccaattcata
    21781 gttgatacgg ccaacatagt cttcaggagt aaaggttccg tctgaaccaa ctggaaggtt
    21841 acgcatgagg tagtaacgaa gtggatctag tccataacgc tctaccaaca tttcagggta
    21901 aacgacattc ccttttgact tagacatttt tccgtctttc atgacaaacc aaccatgggc
    21961 aatcaaacga tcaggtaatt taacatccaa catcataaga aggattggcc agtagataga
    22021 gtggaagcga aggatatctt ttcctaccat atggaagact gttccattcc agaacttgtc
    22081 aaagttacca tgttcgtctt gagcgtagcc aagagctgtc gcatagttaa gaagggcatc
    22141 aatccaaacg tagacaacgt gttttggatt tgatgggaca ggcactcccc atgtaaaggt
    22201 tgtacgagat accgccaaat cttccaaacc tggctcgatg aagttgcgta gcatttcatt
    22261 aaggcgacca tctggcgtga taaattcagg atgagctttg aaaaattcga ccaaacggtc
    22321 ttggtatttg ctaaggcgaa gaaagtatga ttcttcagaa acccattcaa cctcatgacc
    22381 tgatggagca ataccaccag tcacatttcc agcttcatca cggaaaactt ctgccagctg
    22441 gctttctgta aagaattctt cgtctgatac tgaataccaa ccagagtatt cacccaagta
    22501 aatatcatct tgagcaagta agcgttcaaa gacctgtgcg acaacttttt catggtagtc
    22561 atcagttgta cggataaatt tatcgtatga gatatctagt aattgccaga gttctttaac
    22621 tccaaccgcc attccatcaa cataggcttg aggtgtaata ccagcttctt ccgctttctg
    22681 ctggattttc tgaccatgtt catcaagacc tgtcagataa aatacatcgt agcccatcag
    22741 gcgtttgtaa cgtgctagga catcacatgc gatagttgtg taggcagaac cgatatgaag
    22801 tttcccagat ggatagtaaa tcggcgttgt aatataaaaa tttttttcag acataatttt
    22861 tcctttccag gcaaatgaaa cctgtttttc taacacttca ttatatcaca tttttaatga
    22921 atttcgatag ggaaatccat acaaaaacaa gatagacgag tgtccatctt gttgatctca
    22981 ttcataacga agggcttcaa ttggatcaag tttcgatgcc ttgttggctg gcaagactcc
    23041 aaaaatcata ccaacactag ccgaaactgc aagactaaat agggcaactg ggattgatac
    23101 tccaacttct ataccttcta ttaaaccttg cagtaacaaa cctgctaagg cagttaaacc
    23161 acttgcaatt gtcaagccaa ttaagccacc taacaaggtc aaaatcatgg attcaatcaa
    23221 aaactgaatt aaaatattgg cacgtgttgc acccaaagcc ttacgaagac caatctcacg
    23281 agtgcgctct gtcaccgaaa ccagcatgat gttcatgaca ccagttcctc caacaaagag
    23341 agaaatccct gcgatggaac taataatcgt cgtcataaaa ctaaacgatt gttgaatttc
    23401 tgcaaataca acggactcat ctgccacctg gtattctccc tgttgtaagc ctgcaagctc
    23461 tgtcattttt cgtgccagtt ctggacccag agttggggtt aaactggtat cattcactcg
    23521 aaagacaata ttagctattt catctacatt aaaattcgca gcaagggaga tattggtagt
    23581 aataggcaag ccaccaaacc catatatttt tgatctttta gcctccggac tagtataaac
    23641 cccaatgacc cggtaactaa atccattgac ttctacaacc ttgttaatag cctcttgagg
    23701 agattcaaat aaactaatgg acaattcctc atctagcaaa atgacacttg caaactcttt
    23761 gaaatcttgc tctctcagac tacgacctgc aataatttca ttcttaacag cgtccatgta
    23821 agttctgttt ccacctgtca aattagcatt ctcaaccttt ttatcttgat aggtcaagat
    23881 ggcattcgtt gaattggtta catagtaact atccactccc ttcagtttag ctgcctcttg
    23941 gacccaggat tcttgcggtt ttggcggttc aacaggaact tcctcttcct ttccagaaac
    24001 cgtaaaagct gattgtttct gagtaaaaga cccatcttta ctttttttag gagagaaaaa
    24061 gacgctaata tttttctgag atttagtcat atctttattg acttgacgag atagggaatc
    24121 acccaaagcc ataatcacaa caactgatga aacaccgata ataatcccaa tcatagtaag
    24181 caaagaacgc atcttgtgag ccatgataga tgaaaaggca aatttcagat tctgcatctt
    24241 agttttcctc ctttcctaac tgagcactgt cagacgaaat gaccccatcc cgaatgacaa
    24301 tctgacgttt ggcataggca gcaatctcag gctcatgcgt taccatgata atggtttttc
    24361 cttctttatt caaatcaacc aataattgca taatttggtt acctgttttg gtatccaagg
    24421 ctcctgtcgg ttcatccgct aggataatag aaggattgtt taccaaggca cgcgcaatgg
    24481 ctacacgttg cttttgacca ccagataatt ctgaaggtaa atggtgacta cgttctgtca
    24541 attcaacctt gtctaaatat tcctcagcca acttgcgacg ttttgaagac gaaactcctg
    24601 cgtaaatcaa gggcaattct acattttgca gagcattgag cttcgataga agaaagaact
    24661 gctgaaagac aaaaccgatt tgttggttac ggaccttagc tagttgtttt tcaccaagcc
    24721 cagccacttc ttgaccttca agataatatt ctccactggt tggtgtatcc aacatgccaa
    24781 tcgtattcat cagagtggac ttaccagacc cagatggtcc catgatggct acaaattcac
    24841 cctcattcac ttctagattg atatttttga gaacctgcag ttcttggtca ccattacggt
    24901 aacttctgaa gatatttttt agactaatta gttgcttcat cagccttcac ctcttttcct
    24961 tcttccaagg aagatgttgg attactgatg accttagcac cgttcgttaa accagaagtg
    25021 atttcttgat tttctgcgtc agcatttccc aatgaaacct caactttttt agccttttgt
    25081 tgttcatcca caatccagac ataattttta ctatcatcca ttactagact gctaacagga
    25141 acaagaatag ccttagtttt gcttttaacc tcaatgttga cagaaaaacc ttgtttcaaa
    25201 tcaccaacct cgcctgtcac atcaatagta taagggtatt tagaacctgt attattcccg
    25261 gctgctggac tagctgcttc accattgttt ttaggatagt cagaaatata gcttaatttc
    25321 ccagtccatt ttttatcagg atacacttta gaagtaaagc ttacttcttg acctacagaa
    25381 aggttggcta gattgtactc agacaattct cccttgactt gtaaattttc attgctgaca
    25441 atatgaacca taacttgact cgcccctgtt ggagatttag aaacattgct attgacttcg
    25501 actacagttc cctctagggt actgagaaca gttgttgcat ccaattgact ttgagccttg
    25561 cttaattgcg ctgcagcatc tgcacgcgca tcacgggcat cacccaattg agcatcaata
    25621 gaagcaacag aatttccagc cactggagtt gggctttgca ccgttgcatc ttctcctcct
    25681 actggcgctg gtaactgtgg agcctgagct gaagcggctt catttcgtgc ttgattgagt
    25741 tcattgatat gacgatctgc cttagctact gctcgactag ctgaatcata ggccgcctgc
    25801 gcttctgaac tactgtactt gactaaagcc tgcccttcgc tgaccttatc gcccacagaa
    25861 acaaggattt catctaaatc acccttacta gcatcaaaat aaacatattg ttcatttttt
    25921 gctgttactg tccctgacaa taaaacagag gatgccacgc ttccttcatt ggcaacaaca
    25981 agatgagtag gctcatcttt tagagcagtc tgagaaggtt gtctaaagag taaaatcccc
    26041 ccggcaccca atacaactac actcgcagca ccgattgctg catacagttg ccacttttta
    26101 gctttaccat tctttttctt cataatgaaa ctccttttct tttttacaat actttgctat
    26161 tataccaaat ttccctccag caaacaatgc agttcagatg gaaataatgg cagttcagga
    26221 ttaaacaatc gttcggaatt ttgcttttct attgtactag atatataata cactatcttg
    26281 acttatttgg caagcttttt tgcaattttt tgtgcgtagc agatttttgc aatcaaatca
    26341 aaaaaagaat aaaatataac tatcaaaaaa gacactctac tatttaaaag attacaaagg
    26401 agttttcaat gagagaatat gatatcattg ctatcggtgg aggtagcgga ggaatcgcta
    26461 ccatgaaccg tgctggtgaa catggagcca aagcagccgt tattgaggaa aagaaattag
    26521 gtggaacctg tgtcaacgtc ggttgtgttc ctaaaaaaat catgtggtac ggggcgcaaa
    26581 tcgctgagac tttccatcaa tttggagaag actacggctt taagactact gatcttaact
    26641 ttaactttgc aaccctacgt cgcaatcgtg aagcctacat tgatcgcgct cgttcttctt
    26701 atgatggtag ttttaaacgc aacggtgtag acttgattga aggtcatgct gaatttgtag
    26761 attctcatac tgtaagcgta aatggtgaac tgattcgtgc taaacatatc gtgattgcta
    26821 caggtgccca tccaagtatt cctaatattc ctggtgctga gctaggtggc tcttctgatg
    26881 atgtatttgc ctgggaagaa cttccagagt cagttgccat tctaggcgct ggttatatcg
    26941 ccgttgaatt agctggcgta ctccacactt ttggtgtcaa gacagatctc tttgttcgcc
    27001 gcgatcgtcc tttacgtggt tttgattcct acatcgttga aggtttggtc aaggaaatgg
    27061 aaagaacaaa cttaccactt cacactcaca aagtccctgt caagttagaa aaaactactg
    27121 acggcattac cattcatttc gaagatggta ctagtcacac agctagccaa gttatctggg
    27181 ctacaggccg ccgtccaaac gttaagggct tgcaacttga aaaagctgga gtgactctga
    27241 acgaacgtgg ctttatccaa gtggatgaat accaaaatac tgttgttgag ggaatctatg
    27301 ctctaggtga tgtaacgggc gagaaagaac tgactccagt tgcaatcaag gccggacgta
    27361 ccctatctga acgtctcttt aacggaaaaa ctactgcaaa aatggattac tcaactattc
    27421 caactgttgt cttttcacac cctgctatcg gaactgttgg tttgacagaa gagcaagcta
    27481 ttaaagaata cggtcaagac caaatcaagg tttacaaatc aagctttgca tctatgtact
    27541 ctgcttgcac tcgcaaccgt caagaatccc gtttcaaact cataacagct ggttcagaag
    27601 aaaaagttgt cggacttcat ggaattggct acggcgttga tgaaatgatt cagggatttg
    27661 ccgttgctat caaaatggga gcaaccaagg ctgactttga cgcaactgta gcgattcacc
    27721 caacttcatc tgaagaattt gtaaccatgc gttaatcgaa acaaaagaag ctgatataaa
    27781 tatgtctgct tcttttttaa tgattttcga atcaacaaaa aattatccaa cttgactagt
    27841 cacgaaaatc ggctgttaca gtttttgtta ccttgttttt aaaaaatggt tgacatcgac
    27901 tcctatatga gtataatagt tactatacat tagaaaagag gttaactatt ttgaaaaaag
    27961 ctcacgttta tgctatccct gctattgggg ctgctctcat tgctgttttg gcacaaatca
    28021 gtcttccaat tggacctgtt cccttcactc tgcaaaactt tgcaatcggc ttgattgcta
    28081 ctgtctttag accgagagag gctgtacttt ctgctggact ctatcttctt ctaggtgcta
    28141 tcggtcttcc tgtctttgca ggaggtggag ctggttttca ggctttagtt ggccctactg
    28201 caggctatct ttggttttat ctcgtttact ctggacttac ttcctctcta accaacagca
    28261 agagtggtgt tgttaagatt tttcttgcaa acctcttggg tgatgccctt gtctttgtcg
    28321 gcgggattct cagcttgcat ttcctagctg gaatggcatt tgaaaaagct cttgttgtgg
    28381 gggttcttcc ctttatcatt ccagaccttg gcaaacttct ggctattagt tttattagcc
    28441 gtcccctact tcaacgcctt aaaaatcaga cttactttac taactaaaaa aggataccga
    28501 gttatcatga ctcaatatcc ttttttttat tttgaaaact tatactcaat gaaaatcaaa
    28561 gagcaaacta ggaagctagc cgcaggctgc tcaaaacact gttttgaggt tgtagatgaa
    28621 actgacgaag tcagtaacca tacatacggc aaggcaaagc tgatgcggtt tgaagagatt
    28681 ttcgaagagt attagttgcc tttaaaggca tccaccatcg tttgaaattc ttcatttgag
    28741 agagtaatcc ctttgcccat tttagtatgg tctggactcc aagcacgaat atcaaacttt
    28801 gcaggggcac cattaaagct cacacggtta atttccttgg tccaaccttt ttcgttttca
    28861 gaaagagtca acaagtgctc ttcgatttca aatgtaaatt ctgccatttt cttctccttt
    28921 tttagtttca ttagtttatt cgtaaaatct tgtagatttt aggaaaattt tatataatat
    28981 tgatataaaa gaagggaggc caatatgaga cataaattcc agcaagttct agataaaata
    29041 catgattttt taaatggaca tgaccaacct gaccagactg aaaccaactc ccttacagcc
    29101 actattgaag aggctatcca gaaacaaacc gctgttcacc ttatcttgtc tgagacaagc
    29161 tttacaggtg atatcatcaa atatgatcag caaggccagc aaattatcgt gaaaaatttt
    29221 tccaaaaatg tgagccggat tatccgtata agcgatattc aacgcctgcg atttgtcccc
    29281 tcaactgtcc aaacagccca aaaaaataga tttaagaaag agtgagatgt agttgcttca
    29341 tcccactctt tttcttagcg aatttgttca aaatgtaaat gaactgcgat atgatctcca
    29401 taaccacttc tttccaagtc acgttgtaaa cgataggaaa tatagtgttc tgcaatggta
    29461 atgtaacctg cgcccaataa acgatgttca accatagatt gaatcatact gatagtcgca
    29521 cgttccacct tggcttcttg taaatccaaa actaccttct tagtgacttg agcaagattt
    29581 tgacgcaaat catctgtcaa aacataaaca gtttgggctg ccttcaagat ggcttggtaa
    29641 atcttatctg gattaaattc agcaatttcg ccattacgtt tgattacttg cataggtttc
    29701 tcctttattc tttgttttct ttgatttctg ccagcatttt ttcttcttct actgtcagtt
    29761 gataatgttc aagtaaatcc ggtctgcgct cgtaggtttt ctttaaactc tcgtacaatc
    29821 gccactgacg aatcttttca tgatggccac tcatcaatac atctggcacg accatgcctc
    29881 gataatcata gggacgtgtg tactgaggat attctaaaag acctgaagaa aaactatcat
    29941 cttggtggct agactccttg ccaatcactt ctggaatcag gcgaactgta gcatcaatca
    30001 tggtcatagc tgccaattct ccaccagtga ggacatagtc gcccagggaa atctcatctg
    30061 ttaccaaggt cttaatgcgc tcatcataac cctcatagtg cccacagata aagattagct
    30121 cttcctcttg agccaaatct tcagcataag cctgatcaaa ctgctttcca gcaggatcga
    30181 ggagaataac gcgcggattt ttcttttcaa tagcatcaaa ggaatcgaaa ataggttgtg
    30241 ctctgagcaa catgccctga ccgcctccgt agggctcatc atctacatga cgggcctttt
    30301 cagcattttc tcgaaaatta tgatactgga tatccaagag ccctttttct cgagcctttc
    30361 caacgattga gtgctccagt ggagaaaaca tctctggaaa gagggttaaa atatcaatct
    30421 tcatcgtcta acccttctaa gatttccaca tcgacccgtt tatttggaat atcaacattg
    30481 agaaccactg gtgggatata aggtaaaagc aaatcacgtt tgccttttcg tttgaccacc
    30541 cagacatcat tagcacctgg ttgcaggatt tccttgatgg ttccaaccaa gctatcaccc
    30601 tcatagactt ccaaaccgat aatctcgtga tagtaaaatt caccatcgtc taggtcattc
    30661 aaatcttcct cagcgacctt gagactgtat cccttgtact tttcgatagt attgatatgg
    30721 tacatatctt tgaatttaat aatgtcaaag ttcttctgtt tacggtggct agcgatggtc
    30781 actgtttgga caaactgatc tttttcatca aacaaagcca gctcagctcc ttttttaaac
    30841 cgttcttctg caaaatccgt cacagacaag actcgcatct caccctgtaa tccctgcgta
    30901 ttaacgattt tcccaacatt aaagtagttc atcttgtctc ctgtaatctc cttttttcca
    30961 tcttattctg acaattctcg aataatagcc gcaatttttt ccgattctga ccattgtaaa
    31021 taatggtgat tccctcctaa aatgagttta gtattggaag tccaatattc tgattctctg
    31081 tactcttttt ctctataagt ctgacaaaaa ataaatatag ggacatgaga ttctatcgac
    31141 aaattctcaa aatcttcctc agtaatctct ccagatatct gaaattctgg atcttgattt
    31201 tccaactcta agcctttttc ttgcattaat tcccagattt ttttattcgt ttcaggacta
    31261 aatgttgctt gagttaagtt cttaaaataa agttcaggac cacactcgtc aatcagcctc
    31321 atctgctctt ccatttctgg ataaggattt tctgaaaaat cagcaaacat gactttttta
    31381 gttgtcggtt caattgctac taaagtctga cacttaattg gtttctcgag taatttgcaa
    31441 gctaaaattc cactccaact atgtacacaa agtatatatt cagaaatccc taattcttca
    31501 agtgcttcat aaaccacatc tgcaagatta tctagatttt ttccagcttg gtcataaatc
    31561 ggactcctac ctgtgttcgg aaaatcaatt gtcaaataac caattgtagg aggaggtttt
    31621 tcaagtataa gtgaaaaatt ttcataactt ggtagcaaac ctgctccatt tagacaaact
    31681 agcactttct tttgcttttg ataagcaaca gagagactac caatttctgt agatacttca
    31741 aacctcttca taaagaaatc cactgattct atataatgaa ttattaaaaa tccttatcct
    31801 ttattttatc acgttccaag gattttctca agttggagga aggggacaac atctctactt
    31861 tcccttcaag aatccttcca aatcatgttt atgttggtaa ttaacggctg cggttttgtc
    31921 tttctcaaag atggttctag ttaggtcaat atgcttgata gcagcaatag cgacggtgta
    31981 gagaatgata tcagccagtt ctctggcaag ttcctcgttc gaatcctatc ccttcttttc
    32041 gaccagagcg cctattcaaa acctcggcta cttctccgac ttcctccact aacttcataa
    32101 agagaccttc atcagtccga gactgctgtt aatgttcgag taagtagtct tggaattgcc
    32161 taaacgttca atcttttata gtatattgaa actagaatag tacaccttta cttctaaaac
    32221 attgttagaa atcgatttga ctgtcctgat cgatttgtcc tgttattatt tcattttact
    32281 atatcttcta ttccacacaa aaaagcgaga catccgtccc gcccttctta tttttcgtca
    32341 ataacgattc ttactttttt gtattcagtt gggacagagt agacaatcgt tcttatcgca
    32401 gaaatagtgc gacccttacg accgattaca cgacccacat cgctttgatc aagattcaaa
    32461 tgatattcca aaaattctgg tgtatcctca atcttgatag ttaaggcatc tggttgtgaa
    32521 attaagggtt tcacaatcgc aataatgaga ttttcaatcg tatccatctg tcaacctact
    32581 ttaaacttat tttgaaaatt tagaatcgtg gaattttttc aatacgcctt cttttgaaag
    32641 gatgttacgt actgtgtctg aaggttgagc tccattagcc aaccatgtaa gaacgcggtc
    32701 ttctttcaaa gttacttggt tttcagcaac aagtgggttg taagttccaa ctgtttcgat
    32761 gaaacgtccg tcacgtggtg aacgtgaatc tgctacgttg atacggtaga aaggtttttt
    32821 cttagaaccc atacgagtca aacggatttt aactgccatt tttaaagtct catttcttta
    32881 attttttatt tcggtgaaat agctgagcta tttagcacat gttctattat agcagatttc
    32941 tggcaggtgt caagaaaaaa acttgacaga ctataaattt cttaaagcat ttaaaaattt
    33001 gttagaatag gaaataaatg tttgaaagat gctatccgtg aatactattt tagaaaaatt
    33061 ttataaagaa caccaagtca aacccttcat atcgcctgaa cgagatttgg atacttggct
    33121 tctaagtccc aagcccgttc ccaagcgaaa tatggactta ttagtagatg attcactagc
    33181 aggagatatc attttactat ggagaatcca gtttggtact tttaccactg aaacatgatt
    33241 ttaaaaactc ccattcgaat atgattcaaa attcgcttag aatcaatgtt ttcccacttt
    33301 ttcaaaaaat aaattttcat ctaatttaaa taactttcaa ttaagtgtga attttgctta
    33361 aaatcattaa aaaacacccc atggtgtgaa ccatgaagtg ttgtaaatgc tgtgttgtag
    33421 ctagttctta gcgtttagag aactggctag ctttacgagc tttcttaaga tctggtttcc
    33481 aaaatacttt gagtacactt atggtattga tgcccctaaa catctaaaaa ctctggtaga
    33541 aaaagggtat gctcttgtcg aaacttcttt tgactcactt gaccatctaa ataataccat
    33601 gaaaaagaat atcctgaaga agaagggagt tactggactt tctaaaatga aatcttgcga
    33661 cctggatcaa gcccttcatg agtatttttc agaagaagaa ttagctggtc actttcatgt
    33721 ccttctatgg acttttttac aatggcattg ctatcacacc caatacctat ctaagcgcct
    33781 ggttcgtaaa ctttattgca gctcttcctc taaatttcct aattgttgaa ccaattgccc
    33841 gttttatact aagttctttt cagaaaccat ttactgggga agaagttgaa gaagttgaag
    33901 atttttaaga tgatgatgaa atcccaacta ttatctaagc cagttctgta aactactaat
    33961 atttgaaatc cacttccttt tagggtacaa tggttataaa tgaatttttg agaggaacag
    34021 aatgaaaaaa ctagcaaccc ttcttttact gtctactgta gccctagctg ggtgtagcag
    34081 cgtccaacgc agtctgcgtg gtgatgatta tgttgattcc agtcttgctg ctgaagaaag
    34141 ttccaaagta gctgcccaat ctgccaagga gttaaacgat gctttaacaa acgaaaacgc
    34201 caatttccca caactatcta aggaagttgc tgaagatgaa gccgaagtga ttctccacac
    34261 aagccaaggt gatattcgca ttaaactctt ccctaaactc gctcctctag cggttgaaaa
    34321 tttcctcact cacgccaaag aaggctacta taacggtatt accttccacc gtgtcatcga
    34381 tggctttatg gtccaaactg gagatccaaa aggggacggt acaggtggtc agtccatctg
    34441 gcatgacaag gataagacta aagacaaagg aactggtttc aagaacgaga ttactcctta
    34501 tttgtataac atccgcggtg ctcttgctat ggctaatact ggtcaaccaa acaccaatgg
    34561 cagccagttc ttcatcaacc aaaactctac agatacctct tctaaactcc ctacaagcaa
    34621 gtatccacag aaaattatcg aagcctacaa agaaggtgga aaccctagtc tagatggcaa
    34681 acatccagtc tttggtcaag tgattggcgg tatggatgtt gtggataaga ttgctaaagc
    34741 cgaaaaagat gaaaaagaca agccaactac tgctatcaca atcgacagca tcgaagtggt
    34801 gaaagactac gattttaaat cttaaaaacc aaaaaaatac agtatccaca ttcggtactg
    34861 tatttctttt actctcattc ttaagttaaa ttattaaaat cccatatttg gtctatccaa
    34921 ccttcataaa agtctggatc gtggcagacc ataaggatag atcccctgta ttctttgaga
    34981 gcgcgtttga gctcatcctt tgcatccaca tccaaatggt tggtcggctc gtccagcact
    35041 aaaacgttgt tttcacgatt catcaagaga cagaaacgaa ccttggcttg ctctccccct
    35101 gataatactt gaatctggct ttcaatatgt ttggttgtca aaccacaacg ggcaagggct
    35161 gcacggactt ctgcttgatt aagggcagga aaggcattcc agacagcttc aagaggagtt
    35221 tggcgattac cgccttctac ttcctgctca aaataaccaa gttctaaata atctccacgc
    35281 tccacttccc cagcgattgg cgagataatg cccaagagac tcttcaagag agttgttttt
    35341 ccaataccat tagcaccaat aatcgcaacc ttttgattgc gttcgaaggt aagatttaaa
    35401 ggcttagtaa gaggacggtc gtaaccaatt tgcaagttct tggcttggaa gataaagcgc
    35461 cctggtgtac gagctggttt gaaatcaaag gatggttttg gtttctcact ttggagttcg
    35521 ataatatcca tcttatccaa tttcttttga cgagacatag ccatattacg agttgcaaca
    35581 cgggctttat tacgagccac aaagtccttg aggtctgcaa tctctttctg ctggcgttcg
    35641 taggctgcct ctagctgaga tttcttcata gcataaactt cttggaactg gtagtagtca
    35701 ccagagtaac gcgtcagctg ttgattttcc acatgataga caatattaat aacgtcattg
    35761 aggaatggaa tatcgtgcga aatgagaaca aaggcattct catagttttg gagatagcgc
    35821 ttgagccaat caatatgctc agcatccaag tagttggtcg gctcgtccaa cagcaaaata
    35881 tcaggctttt caaggagaag ttttgccaaa agcaccttgg ttctttgccc acctgacaaa
    35941 gaagttacat ccgtatccat gccaaagtcc ataataccaa gagcacgcgc tacttcgtca
    36001 atcttagcat ccaaggtata gaaatcacga ctctccagac ggtcttgaag ttctcctact
    36061 tcttccatga gagcatcaac atccgcgccg tcttcagcca ttttcatata gaggtcattg
    36121 atacgagctt cagctttgaa aagctcatca aaagccgtac ggagaacatc acgcaccgac
    36181 tgtctttcag caaggacaga gtgctgatcc aagtaaccag ccgtcacata tttggaccac
    36241 tcaacctttc cttcatctgg cagcatttta ccagtcacga tactcataaa ggttgatttt
    36301 ccttcaccat tggcaccgac caggccgata tgttctccct tgaggagacg gaaggacaca
    36361 tcttcaaaaa ttgcacggtc accaaaaccg tgactcagat ttttaacttc taaaatactc
    36421 attttaattc cttaccttgt ttttatgtaa tcgtttataa aggagccaag ccagatagcc
    36481 acccaaagtg ttggtccaca aatcatcaat ctcaaagacg cgattgaaat caaagaaaaa
    36541 gtccaagatt aattgcgtac actcgattcc aagactcaca agaaaactaa aaagaaggac
    36601 cttttttgtt ttccgcaaat ttggaaatag ataaaggagt tggaaaatca gaggaaaaaa
    36661 caagaagaca ttgaggatat tttgtaaaaa aatccaacat aattgtccaa tgtcactcac
    36721 ttcgcccagt ttccagagag aattgaaagg agtcaaaaga aaaaccaggc gtccaagatg
    36781 ctgaatacct ggagttccca ctcccacggt agattgttct tgaggagtaa agcaaaaaca
    36841 gacaatgcaa atgctataga aaatgactcc ccagaccaaa atatgattat aagtcttctt
    36901 caccattaag gatttaccgc tgcgactgcc ttctggcggt cacgtttcat tgtgttagag
    36961 cgcaattgtc cacaagctgc gtcaatatct gtaccatgct cttgacgaac cacacagttg
    37021 accccttttt tcttaagcgt atcatagaaa gccaacacgc gctctttggg actacggcta
    37081 tattggtcat gctcactaac tgggttataa ggaatcaagt ttacataaga caatttcttg
    37141 atgttcttga gcaattcagt caattccaag gcttgttcta caccgtcgtt gacttcatta
    37201 agcatgatat attcaaaggt tacacgacgg tttgttgtct caatgtagta ttcaatagca
    37261 gcaaagagtt tttcaatcgg aaaggcacgg ttaatcttca tgatacttga acgaagttca
    37321 ttgttaggtg cgtgaagaga cacggcaaga ttgacctgaa ccccttcatc agcaaagtca
    37381 cgaattttat gagccaaacc tgaggttgaa accgtgatgt gacgagcacc gatagccatt
    37441 cctttatcat cattgatagt acgaaagaaa ttcaagacat tgttgtaatt atcaaagggc
    37501 tcaccgattc ccatgacaac gatatggctg acgcgttcat cctgaccacg ctcatcaaag
    37561 tatttctgaa ccagcatgat ttgcgctacg atttcaccgt tattgaggtc acgttgcttc
    37621 ttaatcaaac cagaggaaca gaaggtacaa ccgatattac agccgacctg agtggtcaca
    37681 cagacagata aaccatagtg ttgacacatg agtacagtct caattaacat accgtcaggc
    37741 aattcaaaga gatatttgac tgtaccatca gcagactctt gcacaatacg ttgtttcaag
    37801 ggattgacca caaactggtc attgagctta gcaatcaaat ccttggaaag gttggtcatt
    37861 tcttcaaatg actgcacacg tttacggtag agccattccc agatttgatc tgcacggaat
    37921 ttcttttctc cctgctccaa tacccattcc tgcatggttt gatgtgccaa actatgaatt
    37981 gagggtttca tttcttctcc ttattctcta ctcacttctg acgaatgaca aaatgacgtt
    38041 gttccttgtc ctctttctga gaatgctggt ctttctgacg acgtctattt ttcttatctg
    38101 cattcgactt tcgtttagtt tgagtcggtt tctttccttt tctagaaggt gtttcttctt
    38161 ccgtcttacg cattttcttg tcaaatgatg ctcgcttagg ggcttcattt tctaagacaa
    38221 aataggcaca accataacta caatactcta aaaggtagtc ttgtaaacga ctgatttttt
    38281 caagtttttc ttctgttcgg tcatccttgt aaaaacctcg taggcgaagc tgttcattgc
    38341 tccagtcccc cacgatataa tcaaacttgg ttaatacttc tgaaaaacgc tgattaaaag
    38401 tcgtcacatc aaaggcatcc ttgatatttt caaccaagga aaaagctatc ccttccgttt
    38461 cgaccttgtc cccgtgtaaa tggaactccg gaccaggaaa cttgttatag ttgtataatt
    38521 caggtgcaat ttcttttcgc atagatatcc tttttccacg attacttaat actttattct
    38581 accataattt ctagcagtta gcacgtttct cataaaaatg aaaaaagtct gacgattttg
    38641 tcagaccaga atcttataac ctaaaaaaga gaagaacaat tcttccctcc aactatcatt
    38701 atttagcagc tgcgtacaat tcatctactt tattccagtt gattactgaa aagaaagctt
    38761 tgatatagtc aggacgcacg ttgcggtatt tcacgtagta agcatgttcc caaacgtcca
    38821 agcccaagat tggtttttta ccttctgaga ttggtgtgtc ttggtttgct gttgaagtca
    38881 cttcaagttt cccttctttg ttgacaacca accatgccca acctgaacca aaacgagttg
    38941 ttgctgctgc agtgaaggct gcttggaatt cttcaaatga accaaatgtt gcatcgattg
    39001 ctgctgccag ttctgctgaa ggagctgttt tctcgggagt catcaattcc cagaaaagag
    39061 cgtggttcaa gtgtccgcca ccattgttga taagtgcttg acggatatca gctggaatag
    39121 attctacatc agcaagcaag gcttcaaggt cttcaccgat ttcagggtgt ttttctaaag
    39181 ctgcattggc attgttgaca taagtttgat ggtgtttgtc atggtgcaaa tgcattgttt
    39241 ccgcatcgat gtatggttcc aaagcgtcgt atgcatatgg aagttctggt aagataatag
    39301 ccatctgtaa tacctctttt tctttctata tgaaaatgat aacgcaaaca ttcacttttt
    39361 tcaagttttt tgcttataga taaggaaatc actgacatac tttctaataa aataagcaaa
    39421 tcagcaagaa acccacgaca agataatcct gacgcgggtt gtagtttcaa caaatgtcaa
    39481 ttgacctgac tagcaatctg taagagtgcc ttttcaaaaa ggaaaccttt ttcataaaga
    39541 cctgtcttaa tctgatagtc tgtctcaatc aaataggaaa tagcttgctt cagaaagctc
    39601 aaagaaagtc ctctcgaatc tcttaatgca aacttgattt gataaggatt tgggttacgt
    39661 cccagataac tacctaaact acttgcaatc tgcgattctg tttggccaga ctccgccaaa
    39721 atcttcacct gagtaaaagt ccgaaattgt cccagcatga ctgcaatcag tttgatttca
    39781 tcttcccctt gcaaggtcaa gtctctcacc aaatcgcgcg cctgatccat ctttttagtc
    39841 agaataaact gagttaaatc aaaaatattg tcctgcaagg tcttgggaat tgcgttaaca
    39901 atatcctctt cctcgataac agaatccgcc ttataggact gtaaaaagag aagatttttc
    39961 tggatttcgc taaattgaaa ccccgacttg atgaggagat tttcaaaaga atgattggta
    40021 aactgcagac cttgtttctg actccacttt tggaaatact ggcgcaattc ttgttctttt
    40081 acttctactg catcgaagac cttggcatca cgcttaagta atttgactaa ccgtcttttg
    40141 ctatccagct ttccttctgc aaagattatc aacttggttg ttggagaagg attgtcaagg
    40201 tattcctcaa atgacttaag ctcatcatct gtcaaaaagc gtttcttagc agtcgtgata
    40261 tccataaaat aatctaatat cacgattttc tcatccgcaa agaaaggaag gctgaccaac
    40321 tccagttcca catccttgta aactacttct ttcatatcaa agtaggcaaa gttgaggtca
    40381 gcagaatcat acccaatctg tttcaacact tgactcttca tcacttcaaa ctgaccctga
    40441 tctgtccctg taaataggct caggctcggt aaatttgata aagtcaactt ctgactttct
    40501 tcaatggcta gcatcgtctc tcctttcttc agatttttcg atttaattta gtcaatatag
    40561 cgcaatttcc cacggaaatc ttctaagctc tcgtagcctt tttccaccat gattgctttc
    40621 agttcattgg taatgcggtc aaaagcactg acgccttctt tgtgaagggt cgttcccacc
    40681 tgcaccatac ttgctccaca gaggatgtgt tcaaaggcat ctcgaccagt cagaacgcca
    40741 cctgttccga taatttggat ttgaggattt aaacgttgat aaaaggcgtg aacattggct
    40801 agagcagtcg gtttgatgta ttctccacca attccaccaa aaccattctt aggccgaata
    40861 acgacagatt cgtcttctat atagaggccg tttccgatag agttaacgca gttgacaaac
    40921 ttgagcggat atttgttgaa aatagctgcc gcttgatcaa agtgaacaat atcaaaataa
    40981 ggtggcaatt taattccaag aggtttggtg aagtaagcaa acacttctgc caaaatccgg
    41041 tctgttgtct caaaatcata ggcaatctga ggtttacctg gaacatttgg acaggaaaga
    41101 tttagctcag tcagaccacg aaaatcactc tcttggactt ttttcaaaat agtatgggtt
    41161 tcctctggag acatgccgac cagagataag aagaaagttc ggttcgactc tttttcctgc
    41221 aaatctaaaa gataatccaa ataatagtct aagccattat ttggcaagcc catagagttg
    41281 atggaaccaa gtggaacatc ttggtagcgt ggctcaggat tcccctgacg gaagtccaag
    41341 gtcgctgtct tagtaacaaa ggttcctgcc gctgagtttt tgacctcttc taactcctct
    41401 atcgtcatac aagccacacc tgctgcattc atcaagcaat tgtcaaactc aaaaccagca
    41461 atttgtgttt tcgttgatac catgattcta atcctccaga tttcttttat tgctaatatt
    41521 ataccataat ttcaattcta ggtttactag attctgtggt tctatctatt tattcggaaa
    41581 agaaagtcta gtacagaagt catgctatgg aaacagctag tgtattagaa gtttgaacaa
    41641 ggcttgggta acagactttt gaatgaagtc caagcaacaa ggagaaatct caaccatgag
    41701 tccattccat gactctgttg ctctagaaaa ccttgcaata gtcaagtgat aacgaataaa
    41761 actttggatt gaaatatccc caagattatc tattacgagg agaaatctta acctttatca
    41821 tttttaaaga aatatagtaa aatgaaacaa gaacaggaga aatcgatcag gacagtcaaa
    41881 ttgattgcta acaatgtttt agaagttgag gtgtactatt ctagtttcaa tctactatac
    41941 atgtaaatct ctactttcgc aaaatggatt ttcagaacag tatcccagta tagttaaaaa
    42001 ctataaaaaa gagaggacgt tcccctctta aaatcttatt ttactgcttc tttcaaagca
    42061 tctaccttat ccaaacgttc ccatggaaga tcaatatctg tacgtcccat gtgaccgtaa
    42121 gccgatgttt gacggtaaat tggacgcttg aggtccagca tttggataat ccctgcaggg
    42181 cgaaggtcaa agatttgacg agccgctttt tcaagttgac tttcagctac tgttcctgta
    42241 ccgaaagtat cgatacgaac agaaacaggt tgcgcaacac cgatagcata ggccaactgc
    42301 acttctgcct tcttagcaag gtctgctgca acgatattct tggcaatata gcgagccgca
    42361 taagaggctg aacgatccac cttagtcgca tctttaccag agaaggcacc accaccatga
    42421 cgagagtagc caccataagt atctacaata atcttacgac cagtcaaacc tgagtcccct
    42481 tgaggaccac cgattacaaa acgacctgtc ggattgataa agaattttgt cttatcatca
    42541 agataagaag atggaataac ttctttgatg accttgtcaa tcacatcttg atggatttgt
    42601 tcattagtgg cctctggatc atgctgagta gaaataacga ctgtatctac acgtaccgga
    42661 cggtcatttt catcgtactc aactgtaact tgtgattttg catctggacg gagatagcta
    42721 atttctccag acttacgaag ttctgccaga cgacgaacca atttatgact gagtgcaatt
    42781 ggcaatggca tgagctcttc tgtttcatct actgcaaatc caaacatgag cccttggtca
    42841 cctgctccaa tcaagtccag tggatcttga tcagcatttc cacgaacctc caaggcttcg
    42901 ttaacacctt gagcgatgtc aggagattgt tccaccaaag atgggtgtac tcccaccgtc
    42961 tcagcagaaa atccatattc tgtattggta taaccaatct ctgcaatggt atcacgaacc
    43021 acacggttaa tatccacata ggcatttgta gaaatttcac caaaaacgtg gacagaacca
    43081 gtatatacag ctgtttcagc agcaacgtgc gcctctggat cctttgctaa aatagcatcc
    43141 aaaatcgcat ctgaaatttg gtctgcaatc ttatccggat gcccctcaga tacagattca
    43201 gacgtgaata atttacgctc tgacataaaa atgtcccccc ttaaaaaata ttgttataga
    43261 gttacaaagt actgatagga aaactcttcg taataaatac ctaccatctt atcgggacta
    43321 tataacttta tcattatacc atttttaatc gcaatttgca gtctttaatg tagtgtttcg
    43381 gtttagatag ttcgttctca taaagcaaaa agttggagct caatactcca acaatttcta
    43441 ttaaaatgtt tggcgtttag ctttacgctc tgcacgaccg cgagcgcgat tttcagcacg
    43501 cttggtttta cggcgctttt catcaaccgc ccattgaatt ttcttcttat aacccggttt
    43561 gacttttttc tttttctttt taaccaaacc aatcatttcg atatcaagtt tatcttgttt
    43621 tttctcacgg ttggcacgac gatcacggtc ataggtatct tgaaattccc cgtctttgac
    43681 catcttagga ctaaacttga tttccaattt ctccaactca cggatatccg agtcatcact
    43741 tggctgataa agggtaatag ctgtacctgg taggccattt cgtccagtac gaccaacacg
    43801 atgaacaaaa aaagataagt cttgcggaat ggcatcattg atgacatggc tgacaccttc
    43861 aatgtcaatc ccacgcgctg ccaaatctgt tgcgacaata tactcaaaat ccagattttg
    43921 cacctgattc atgattcgct tgcgttcacg aggggcaata tctccatgga tttttgcaac
    43981 cttcaagcct tgagcagtca gatatgaatg caattcatca gcacgcgttt tagtgttaac
    44041 aaaaatcatt gccaaatacg gctgcatcaa ctgagtcaac tggtaaattt gagcattctt
    44101 atcatgtccc ttggtcgaaa tcaaccaatt atcaatggtg tcagaaataa ccgttttggt
    44161 cttaattttc tccataacag gatttgataa gtattttttc aagaatggtt gcagtttttg
    44221 tgggatagtc gctgagaaga ccatgaattg caagtctttt ggaagactgc cagcaatctt
    44281 atcaacagtt tccaagaatc ccatatccaa ggtcatatct gcttcatcaa caacaaatgt
    44341 cttggcttta tgaatagcta aatcaccaga tttaaccaag tcgtagatac ggcctggtgt
    44401 tccaataaca atatgaggct gattgcttgc caatttctca atctggcgag ccttatccgt
    44461 accacccaca taattaacca cacgaacttc gacatctgag tgagctgaaa tctgacgcgc
    44521 tacttggtaa atttgagtag ccaactcacg actcggtgca gtaatcactg cttgcacact
    44581 atcgctagct tcatctaatt gctggaaaat cggtaacaag aaagtatgag tcttacctga
    44641 acctgttttt gattctccta ctaggtcacg acctgccaaa acaataggaa tcaacttgtc
    44701 ttgcacctct gttggagttg taaattttaa ctccttcaag gcttctctaa tatagttttt
    44761 aaattgaaat ttcgtaaatg acataacatc ctcgattcta tctatcttat caattatacc
    44821 atattttatt ccattacagt agtctcactt atttaggcta tttccagtag cttctctagt
    44881 aagaaaaggc tggaatttta tagttccaac ctcttttcag ttattatttc cagtttaaca
    44941 tagcattcaa gccatagtga tcactcactt gtggactctt gttaccatca aatacgacat
    45001 gtaaattttc caccgctaac tctttggtag taaagacata atcgattcga aggggttcag
    45061 tgttcccttt ccagccatca atttcaggcg gaacagtata gctaccactt ttctcttgag
    45121 caacttcaaa tgcgtcttgt aagcctaatg gactagctaa aatagcttgg taaccttcct
    45181 gacctgctgg gttgttaaaa tctccagcta gtaaaagtgg cttgttcaat tttttcaaga
    45241 cagcctcaaa tcgtgcccat tcttcttgga aacctttatc ccaccaagag agatgaacac
    45301 tggcaactgc tagctccttg ccatcgacta cagtttcagc tagggcaaca cggcgagtat
    45361 gatagtctgt tggatcatcc acatccgaaa ccaaaatttc tctggcttca ataggtgttt
    45421 tagacaagat agccacacct tcatggtagc ggtcatagcc gatatggtta taagcccagg
    45481 tccagtagta atttttccct tgctctaaca acttttcaac caagagtcta acataatggt
    45541 cttggtgaat aggctcagct gctggcaaag cttgataaag gtcattaacc tccacctctg
    45601 acgaggtcat ctcctgattg atttcttgga aacaaatcaa atcatagtcc ttttcaagaa
    45661 tatcttcaag caaaatctgg aatttttcct ctgcttcttt ctccatccaa ctgtgagtat
    45721 tgagtgttaa aaatttcatt cttttctccc aaaataagag gttggaatac agcttccaac
    45781 ctcaagtata ttacaattct actttagcaa ctgctgtttt agctgcaaga gaacctgttt
    45841 tttctaattt aactgattta attgcatcac catttgtgaa gacaactact gttgaagttt
    45901 cacgtcctgc tgcacggata gcatccaagt cagctgtgac aaggagatct cctgctgcaa
    45961 ctttttgtcc ttcagcaaca tgaactgtaa atggtttacc ttcaagactt actgtgtcca
    46021 aaccaatgtg aaccaatact tcaagacctg cttccgtcac aataccaaaa gcatgttttg
    46081 ttgggaagat gcttgataca gtacctgaaa ctggagatac aatgtttcca tttgcaggtt
    46141 ctactgcaaa tccatcaccc atcatttttt gagcaaatac tggatccttt acttgttcca
    46201 aagcaacaac ttgaccgtct gctactgagt aaacttcctc agtaagatct ttgaagtgaa
    46261 cagtgttttg ttgtgcttca gtcatttggc ttggaagagt ttcaggaatg atttcacctg
    46321 aatcaaggat atcttggata tcagatttca aaatgtcagc ttttggaccg tagatagctt
    46381 gaaccccttg tcctttcatc acaagaccca tagctccttc tgctttccat tgctctgcat
    46441 ttcctacttt atctgcatct ttaacagtta cacgaagacg agtcatacat gcatcaacat
    46501 caacgatgtt tacacgtcca ccaagaaggt tgataatgtt tacagcttga gagcctgctg
    46561 caactttcac ttcgctgctg gtttcttctg aaccttcagc agtttcgtag tttccgttgc
    46621 gccctggagt tgcgtagttg aatttttgaa tcatgaagtt tgcgataaag tacatgatta
    46681 cagcaaagag aacagttacc caaacgaagt taacgatatc cataccaata ccagcactga
    46741 ttgcaatagg tgtacgagtc aagaactcga ttgaaccgaa tgagtgcata cgtaggttta
    46801 cgacgtcagc catagcgaag gcagcacctt gaacaagtga gtaaacaaga tacataggtg
    46861 ttgcgatgaa catgaacatg tattcgattg gttcagtaac ccctgtcaag aatgttgcaa
    46921 gagctgttgc aatcatcata cctttgtatt tatgtttctt gtcagcatca acattacggt
    46981 agatagcaac aatcacaccc atcaagatac cgaatgaacc gatcatttgt ccaactttga
    47041 aacgagctgg atgtactgta tctaacaagt gttggtattg actagcatca gtacctttaa
    47101 ggtttacaag gtctgttacc catgcaagcc atagtgggtc ttgaccgaat acttgagtac
    47161 ctttagctgc accagttaaa atgtcataag taccaccaag agctgtgtag ttcattggga
    47221 tagtcaacat gtggtgaaga ccaaatggca agagcaaacg ttccaaagta ccatacaaga
    47281 atggtgcaag aattggagca gtttcttgtg agttggcaat ccagataccg aagttattga
    47341 tacctgtttg aactactggc cagaaagcag caagtagaat tgcagcgatt gctgaacgaa
    47401 gaataactac aaatggtacg aaacgtttcc cgttgaagaa tgaaagtgca tcaggaagtt
    47461 tacggaagtt gtagtatttg ttgtaagcag ttgcccctac aaaacctgag ataatcccta
    47521 cgaatacccc catgttcaag gctggagctt caagaacact gataaagtaa tcagcaactt
    47581 tgattgaacc accaaagaaa gtagttacca tagcatctgg atttttcaac atatcgcctg
    47641 acacaccaaa gattgtacca gtgatacggt taatcaagat aaaggcaaga ccagcggcga
    47701 aagcaccacc agcacgttct ttagcccagc ttcctccaat ggctagggca aacaaaatgt
    47761 gaaggttacc gataaccccc caaccgattt gctcaagaat tccacctgtg atgacaagtg
    47821 gtgtaaaggt tgggttaatc atcacgatag acttaccgat tgaaatcatc aaaccagcag
    47881 ccggcataac cgcgataact accatcaaag ccttaccgaa tttttgccaa aattcgaaag
    47941 acaagacatt tttgaatgta tctttcatca ttcaaatctc ctttattttt atttaggcaa
    48001 acgttttacg aaaacgtttg caccttctat gatttaatta taccacttta attttttttg
    48061 taaaggcttt tataaaaaaa ttacactttt ttctaaaaaa tttctagtgc gccaaatttc
    48121 aagttcaaat tagccatcaa gaagtcttct ctgggggact tctttggaag ctcttttctt
    48181 ctacgcatct cttttcaaaa actcttgtag caaaaaagga gattttacaa tctcctcata
    48241 aaagcagcta ttttacctaa atcttcaaga atcggcgcat ggatattcct gatcccaatg
    48301 aaccaatgaa aaccccaatc acaaatagta gggcaatcat caacggacta aataaatctg
    48361 gactaatcat ggatagattt tgccctacca acgatttgtt gacagattgg taaacaattt
    48421 gataaacaat aaagaccaaa acagatggtg cgatagctcc caataaaccg ataaaggctc
    48481 cttctaacaa gaacggtcca cggatataac tgtttttagc tccgaccaag cgcatgattt
    48541 gaatttcgcg actgcgggaa ataatggtaa tacgaatggt atttgaaatc aagaaaactg
    48601 cgataaaaat taacaaagca gcaatcccta gtccccaaac acggataaat gaagctaact
    48661 tgaagagtct ttctgtattg gcaccgccat cttgaacctc agagacacct tcaatttttt
    48721 tagcatcttc ggctatagtt tttacatcat ttggagcgtt tgcctctaca atataggcat
    48781 catagagagg attggcatct ccttcaaaga ttttccagtt atctcccatt atctcggtta
    48841 atttttcata ttgttcttct ttacttgaaa aggtaacact tttaaccgta gacatgttct
    48901 tcaaagaatc atataccttg tggtagtcat tatttgtaac agtttgacct tctttttcaa
    48961 ttgtctgact attatcttcc acatcctttc ggatataaac tactacacgg acattatttt
    49021 caatatctgt agctagtttc gctgtattga aaataacaga tgcaaatatt gccaccaagg
    49081 tcaaagtaat catgactgaa ctgacagcag ctactgtcat ccaaccattt cgtttcaaac
    49141 tttttaaggc ttcaaataaa tggcgaaaaa atctactaat catcgtatcc atactctcct
    49201 tttgattcgt cacgaacgac acggccattt tcaatggcaa tgacacggtg gcgcaaggta
    49261 tttacaatct ggctattatg agtcgccatc aaaatagttg tcccttgtag gttaatccgt
    49321 tccaagagat tcataatttc ccatgaatta tccggatcca gatttcctgt tggctcatca
    49381 gctatcaata ctttgggatt atttacaatt gcacgcgcaa tcgcaatccg ctgttgctcc
    49441 ccacctgaga gttcatttgg gaaagaacga accttatgct tcaatccaac caagtccaaa
    49501 acttccatca ctcgtttttt gatattacgg cgattttccc cgattacttc catagcgtaa
    49561 gcaatatttt catagacagt tttctttggt aacaatttat aatcctggaa gacaacccca
    49621 acactacgac gtagaagcgg gacatctttc tttttgatct taaccagatt aaaaccagca
    49681 actgataggc ttcctttatc gatttttact tcacgataca gagaacgaat aaaagttgac
    49741 ttccctgctc ctgaaggtcc tacgatgtaa gcaaattccc ccggttgaac gctgaccgaa
    49801 acaccgcgta gagcagtcgt tccgttgtcg tattttttaa cgacatctct catttcaata
    49861 attgacatgt gagttccttt ctatcttagc taattcgcca cttgagataa gcatcgataa
    49921 aaccatctag gtccccatcc ataaccttat ctacctgagc aacctcaaag ctagttcggt
    49981 gatcttttac catagtataa ggcgtgaaga cataagaacg gatttggctt ccccaagtga
    50041 tctccttttt ctcacctttg agagaatcta cctccgcagc cttcttatct tgctccattt
    50101 gatagagctt agcctgcaac atcttcatgg cacgatctct atttccatac tgggtacgat
    50161 ctactgttga ttggacaaca attccagttg gaatgtgggt taaacgtaca cctgttgaaa
    50221 ccttattgac gttttgtcca ccggcaccac ctgaacggaa ggtatccatc ttgatatcat
    50281 cttcacggat ttccacttca atagtatcat ccaattctgg catcacttct acagatgtga
    50341 aagaggtatg gcgacgtttg gcagagtcaa atggtgagat tcgcactaag cggtgaacac
    50401 ccatttctga cttgaggaga ccataggcat taggcccttc aaatgataaa gttaccgact
    50461 taataccagc ctcatcacct gcttggtaat ccaacacttc cactttaaag cctttagcat
    50521 taccataacg agtatacata cgaagcaaca tatcacccca gtcctgcgcc tcagtaccac
    50581 cagaacctgg atggatttcc aagatggcat tgttgtggtc ataaggttct gacaagagta
    50641 gagtcatctc gtagctggtc attatcttat caagttcggc taactgcgct accagttcat
    50701 catgcactga ctcgtcttca gccaaaaaat ccaataaaat ttcgacttca tcctgcaact
    50761 cttccatctt atggaaggta ttgtaagtgt tttttaattc atttaattct tgcgacgttt
    50821 tttgggccgc aatattatcg ttccaaaaat caggttctgt catcttgttt tccaagatgg
    50881 caatctcttc ctctagccct tcgaggtcaa agagaccccc tgaaagaagc taatttttca
    50941 cgatttgcgt caattttctg acgaattact gaaatgtcca taaatactcc ttttttacat
    51001 tatttcatta tagcatattt tatcatttct ttccatcttt tgctctaatc cccttttcat
    51061 gaaaaaagaa gcctttgggc ttcccttctt acaagacttt tgctgaagtg cgagcgattt
    51121 cttccacttg aataccattt gcttcaaatt tttctttcaa ggctggtaaa tcaattgatc
    51181 catcgatttg cacttcgata atcaccttac catccttacg cggaatattg actgtatggg
    51241 agatattcaa attttcttct acaatcaaag aaacaatttt tccaagaaca ccaacttcat
    51301 cttctgtaac aaagcgtaca cgaatccctt cttcgccata acctgcaatt tcaagaaagg
    51361 cttggaaaac gtcacggtca gtaataactc cgtatacttg atggttatct acgacaggga
    51421 gaatactaat cttatttttc aacatcagat aagttgcatc ttctagacta gcatagcctg
    51481 agacagtgac aacatcgcga atcatgacat cttttacttt tgtcttattc agaagataat
    51541 tcatctcata gatagaaaga cttgttgctt tagatggact tgcttgtgca atggttccct
    51601 cagtcaccaa accaactaat tgatcatttt cgataacagg cagacggtgc aaaccttgct
    51661 ctctcatcaa atctgctgca tgagatactg ttgtatctgg actaatataa actaccttgc
    51721 gggtcataaa atctttaact gccatgagac ttctcctttt ctattcttat ccctattata
    51781 caatcttttt gtaaatctat caaacgctta catttctttt tcgaaattca gaaaagtatg
    51841 aataagctag caaaaagagc tctgaaatcg agctctgtga atgaaggtaa gaaatttttc
    51901 attcgatttt ttcaattatt agaaattaag ctttcaatgt agatgcagat ttgtttgcaa
    51961 tctcaatcta ccgactaaaa aggtcccccg gactatattg atgattactt cgtaatctcc
    52021 atctgccgac taaaacaatc cactggattg ttttagccac ctagatatgc ttttctgact
    52081 tcttctgatg aagcgagttc ttttcctgtt cctgatagga cgattctccc tgtttccagt
    52141 acatatcctc ggtcagagat tgcaagtgct ttattggcat tttgttcaat caagaggacc
    52201 gttgttcctt gcttctgaat atcttgaatg atatcaaaaa tttcttggat aaagattggg
    52261 gcaagtccca ttgatggttc atctaaaaga agaagttttg gtgttgacat gagggcgcgt
    52321 cccatggcaa gcatttgttg ttccccccct gaaagagtgg ctgcatcttg gttcttccgt
    52381 tcttcaagac gaggaaagcg tgagaaaacc ttcttcaagt tagcttgatt ttcttcacga
    52441 tttttcttta agaaagctcc catttcaaga ttttccataa cagtcaagcc aggaaagacg
    52501 tggcgtcctt ctggaacttg tgaaagacca cttgccacga ttttctgagc tggcattttt
    52561 tggatttctt gacctaaaaa ttcaatcttt cctgaacttg gtcgaaccaa acctgacaag
    52621 gtgcgaagaa tagttgtctt acctgcaccg ttggcaccga taagggaaac aacttctcct
    52681 tcattaactt caaagcttac atcacgaact gcttggatca taccgtaatg cacagaaaga
    52741 ttttcaactt ttaacataga cattaggctt cacctcctag ataagcttcg ataacgcgtt
    52801 tattggtctt aatttcgtct ggagttcctt gagcgattaa acggccatat tcaagtacgt
    52861 agatacgttc tgttacttcc atgaccagat tcatatcgtg ttcaatcaac atgattgtaa
    52921 tcttaaactc atctttgata cgacgaatta actcagtcaa ttcggctgtt tcctgtgggt
    52981 tcatacctgc tgctggttca tctaagaaga gaattttagg ttccgtagca agggcacgaa
    53041 caatttccaa acgacgttgt tgtccgtagg aaagattttt agcaagagtc tctgcatcac
    53101 catctaaatc aaagattttc aacaattcca aagctttagc ctttaattct ttttcactct
    53161 tgtaaaaagc tggtaagcgt aagaaactag taaaaacatg ctgtttgtga tggtttccaa
    53221 aagcaatcaa aacattatct aaaactgtta aatctttaaa gagacggata ttttggaaag
    53281 tacgtccaag tcccaaagag gcaatcttat aaggtgattt cccattcaaa aggtgaccat
    53341 ctagggttac tgttccctcg cttggttcat aaacaccggt caaaaggttg aaaagggtgg
    53401 ttttcccagc tccgtttgga ccgattaatc caaccagttc cccttcgttc aattcaagag
    53461 tcacatctcc aacagctgtt agaccaccaa aatgtttggt taactgtttt acttcaagta
    53521 atgccattag ttttgttcct ccttcttaga ttttttaaag aaacgtgata ggctcagttc
    53581 ccacgttcca aggagtccac ctggtctgaa aatcattacc aataccaagg ccaaagcgta
    53641 aataatcata cgcacactag caacatcttg gagaagcata ttcaaaattc ccagaacaat
    53701 agccgaaaca atcgcacctg taatggaacc gagtccacca aatacaacaa taatcaaaac
    53761 gttgattgag ttgatgaagg tgtaatcttt cggtacaaca gacccgataa atcctgcctg
    53821 aagtgaccca gcaatacttg cagtaatggc accaaagaca aaagcgatga ttttaatttt
    53881 agtcgtatta accccaactg actcagcagc gatttcatct tcacgaacag agagggttga
    53941 acgaccaatt gggctacgca agaagttcaa ggttgcaatg gttgtaatca cgacaaagaa
    54001 gtaaaccatt tgccaagttg taaagttagg aatccctaag atacctgccg caccatttgt
    54061 aaggcttcca ccattgatga taaagatgcg gataatttca gaaacaccta gagttgctac
    54121 cgcaagatag tcccccttca agcgcaaggt tggaatgccg acaagtaagg caactgctcc
    54181 tgaaagcaaa gcccctacaa gcatagctcc aaagaaggca ccgtaggttg gtgatttaga
    54241 accaataata gctgctgcat aggcaccaat cgccatgaaa ccagcatgac caagtgaaaa
    54301 ttgtcctgaa aaaccaacga ttaagttgag accaacagcc aaaataatat taattccaat
    54361 ttgttgtaaa atctgtacat agaatagatt aagtactccg actgaaacca gtacactaat
    54421 caagctatag ccagctaaca aaaggagtaa ccatagaata ttaactttta aattttcctt
    54481 catcgtttac accttctctt tcacattctt accaaggata ccagctgggc ggacaatcaa
    54541 gatcaacaac aagattccat aaacaatggc atcacggaaa tctgacatcc caaaggctgt
    54601 cgcaaaggtt tccaatagac caatcacaaa gccaccaaga gccgcaccag gaataattcc
    54661 gataccacca agtactgcgg caacgaaaga tttaagacct ggagtaaccc ccatcaaagg
    54721 ctcaagagag ttataataaa gagcaatcag aacaccagcc gcacccgcaa gagcagaacc
    54781 caaagcgaag gtaaagctaa tcgtacggtt tacattgatc cccatcaatt gtgccgcgtc
    54841 gctatctact gatactgcac gcatggcttt ccccatctta gtcttttgga caatgacttg
    54901 taacaaaatc atcaaaatca aggaaatggc caaaatcatt aactgcacat ttgttaagct
    54961 aattggtccc aaatcatatc gaactgtttg aatcgcttga gggaaggcac gggtattggc
    55021 accaaccaga tagaccattc catactccaa taggaaagaa accccaatag ccgtaatcaa
    55081 aacagcaata cgagtagagt ggcgcaaagg tcggtaagca agaaactcaa tcacgacacc
    55141 aagaatagct gtcactagca tagctacaat aagcgctaca aagaaattca tttggaaaga
    55201 attgatcaag aaataaccga taaaggctcc catcatataa atatcaccat gggcgaagtt
    55261 gatgagcttg ataattccgt aaaccatggt atatcctagg gctaacagcg cgtaaacact
    55321 acctagaatc aaaccattta cgagttgttg gagcataaga ttcactcttt ctatttataa
    55381 ttccgagggt tttccctcac tttttgatag gttcttatac tcaatgaaaa tcaaagagca
    55441 aactaggaaa ctagccgcag gttgctcaaa gcactgcttt gaggttgtag ataagactga
    55501 cgaagtcagt cacatatata atccaaggcg acgttgacgc agtttgaaga gattttcgaa
    55561 gagtattaaa tatcgaaaca gggagtgagt caaaggctca ttccctattt caacattttt
    55621 ctattatggt tttacaactt ctgctgcttc aactttacca ttgttcatgg tcatcatgta
    55681 agcagttttg actgtgttgt ggtctgcatc gaagcttgtt tgaccagtta caccttcaaa
    55741 atcttttgtt ttagcaaggt tattcttgat ttcacctgaa tttttagcac cttttgctgc
    55801 gtttgctaca aggtgaactg aatcataagc caaggctgca aatgttgaag gctcttcatt
    55861 gtacttagca cggtaagcgt caaggaaggc tttagcttta gctgaaactt ctacagtagt
    55921 tgagaagcct gagataaagt agatgtttga tgctttttca gcagttgctt gttgtacaaa
    55981 ctcctcaccg ttgaatccat caccaccaac gattggtttg tcaattccca tgccacgcgc
    56041 ttggtttaca attttaccag cctcattata gtaaccagga acaacgatag catcaaagtc
    56101 tttccctttc atttttgtaa gggctgcttg gaagtctgtg tcacctgcta cgaaagtttc
    56161 atctgcaacg atttcaccct tgtatgactc gcggaaagat tttgcaatcc ctttagcata
    56221 gtcactggca ttgtcagtgt aaagaacaac tttcttagca tttaattttt cagaaacata
    56281 gtttgagata atttttcctt ggaagctatc ttggaaagtt ccaataaaga ggtaatcttg
    56341 acctttagtc aatccatctt gagtcgcact tggtgagatc aatggaacac ctgcttttgt
    56401 agcgttcgct accgcagctg cagtcgcacc agatgtcgca ggtcctacga ctgctgatac
    56461 tttagattgg gttacaaggt tagttgtaac tgaagcagcc tcagctgttt cagacttatt
    56521 atctttatcg actacttcga tttgttttcc atcgatacca cctgcggcat tgatttcatc
    56581 aacagccaat tgggcacctt tttgttcagc tgttccgtat gcagctaaag aacctgattc
    56641 ttcaaagtta aacccgattt taattgtctt ttcctctact gagttaccag cagtgttgac
    56701 tgctccagac ttcacttctc cacaggctgc aagaagtgct acacttgcaa gcgccacaaa
    56761 cgatagggca aattttttct tcatcttttt gtctccttat ttcttaaata gtctgttaag
    56821 aatataccga attatcagaa aattgtcaat gttttccttg aactttttcg atgataacgt
    56881 tttcctatcg ataaagattg cccacaaagg gtgtttccaa ctcttggata tgacaaaccc
    56941 taactttttt aataaatttt tccttgctca agcgtccgac caattgctcc acttcttgag
    57001 ttggaacata gagttgtaag taacgatgtt tcttggaatg ataggtaata tctccataat
    57061 cctgcagttt tttggcatca cgattatagt aaagatagat aatcaagcca gagcgattga
    57121 ctttttcaaa catgataaat cctctttcta agaaatctct ccctattata ccaaaaaaag
    57181 caaacccagt cgagtttgcc ttctatcatc attagttcaa tgaattgttg gccataattt
    57241 catcaataaa gccatattca agtgtttcct gggcgctcat ccagttatca cgttctgcat
    57301 ctgcatggac tttttccatt gactgacctg aattttcagc caagattttt tccaaggtat
    57361 tacgagtttt gagcaagtgt tctgcagcga tagccatatc agtttgttgg gtaccaccac
    57421 ctgtaccgcc cattggttgg tgaatcatgt attcagcatt tggaagcatg aaacgtttgc
    57481 cttttgctcc acttgatgcg atgactgttc ccatagatgc agccattccc ataacaatgg
    57541 tttggacatc tgccttgata aagttcatgg tatctacgat tgccaaacca gctgaaacag
    57601 aaccacctgg tgtattgaca taaaggtaaa tatcttttgt actatcttgg gcatccaaga
    57661 aaagcaattg ggcaataaca gagttagcca tattgtcttc aaccggacct gtcagcataa
    57721 tgatgcggtc tttgagaaga cgtgagtaaa tatcgtaaga acgttctcca cggcttgttt
    57781 gttcaataac tacaggaatc attcatttct ccttttgagt tttaattttg ttggtcaaat
    57841 gactgaagat aagactatta taatatcttg gtcaaaaaag gtcaaatttt tgctctgctt
    57901 tcattagaca gaaacaaaaa cccaacctcc tttcgtgact ggaaatactt ttccaagtca
    57961 ttcttctttt cgatcttatt ttgtaccgaa caagcggtct ccagcatctc caagacctgg
    58021 aacgatataa ccgtgttcgt tcaaacgttc atccaaggct gctgtaaaga tttctacatc
    58081 tggatgagct tcttgaaggg cttttacacc ctctggagca gatacaaggc agacaaattt
    58141 gatatttgat gcgccacgtt ttttaagaga atcaacagcc aagattgctg agccacctgt
    58201 tgccaacatt gggtctacta caaaaatttg acgttggtca atgtcctcag gcaatttcac
    58261 caagtattca actggttgaa gtgtttcttc atcacggtac ataccgatgt ggccaacttt
    58321 agcagctgga accaagctca agagaccatc aaccatcccg atacctgcac gcaagattgg
    58381 gacgatggcc aatttcttac ctgccaattg tttttgaact gtttttgtaa ttggtgtttc
    58441 gatttccaca tcttctagtg gaagatcacg aagtacttca taccccatca acattgcaat
    58501 ctcatctact agctcacgaa aagcttttgt agaagtatct gtacgacgca agattgacaa
    58561 tttgtgttga atcagtgggt gattaataac ttcaattttt cccatttttg gaattccttc
    58621 tttcaattta ttcttcttat tataccaaaa aacggtttaa aaatctttct aagccattta
    58681 tttttgataa tttttacatt agatcagcct ctttaagagc tgtctgtact gtctcaagtg
    58741 gtaaatgggt caattctgtc cctttttctt gataaaggta ttgggcgtag tcgtccattc
    58801 ggtactggtt gatataaacc acgcgcttgc agccgacctg aagcaattgt tttgtacagt
    58861 tgagacaagg aaaatgggtt acataggctg taaagccttt gggaacacca cgctcagcac
    58921 cttgaaggat agcattgacc tcagcgtgaa gggtgcgaac acagtggcct tcaatgacca
    58981 aacattcgtg atcaatacaa tgctcagtcc ctgacaccga accattgtaa ccagtggaaa
    59041 taaccttatt atcttttacc agaatcgcgc ccactttagc acgtttacaa gtggaacgat
    59101 tcgcaattag tagggcttgg gctgcaaaat actcatccca ggccagtctt ttttcagtca
    59161 tctcttttct cctttttctc tattttttaa aaaatggtaa acctaaatct gcaatctttt
    59221 cagctggtac cttcatgcca tccttgatcc attttagaag gacagagacg atggctgagc
    59281 tccagaagga atgaagataa gagctgacac cttttgattt cccatggtat ttttctagaa
    59341 attcctgcat ggcttggaca aagatttttt ccagatggta atccaaggcc aattgaatta
    59401 ctctagcttc ctttctggcc tcccggaaaa ggtgaaccca aaccaaataa aggtctgtct
    59461 ttaaatcgta atgatgcagc tgttccataa tattgtggac agttcgttta aagacgctct
    59521 ctaaaatttc ctctttggag tcataattgc gataaaaggc cgcacgcgaa acacctgcac
    59581 gtttgaccaa ttcagaaata ctaatcttgg tcagctcctt tttttccaag agttgcaaga
    59641 gggctgtttc aatggcttct ctggttaata aattggattc ttggtttgat tttctgagat
    59701 tttcaagaga cttttcagag attctacgtt cagacataac attttctttc tacttgtcac
    59761 aacagacgga tgatgctttt gtttcaagag ttttcatgat ataattgtaa aaaaagacag
    59821 aacctttgtc aatgtataac tgacaaagat ggagaatttc tatgacttgg aagattattg
    59881 ctgactctgg ttgtgattat cgtcaactgc caacaccagc tattaacaca acctttgtaa
    59941 gtgtcccctt aaccattcaa gtagctgatc aggtctttgt tgatgacgcc agtctcgata
    60001 ttgaccaaat gatggaaacc atgtatgcaa ctgcagaagc ttcaaaatca gcttgtccaa
    60061 gcccagatga ctatttgcga gcatttgaag gtgccgaaaa cattttccta gtaaccatca
    60121 caggtaccct ttctggcagt cacaatagtg ctcaactagc aaagaatatt tatctggaag
    60181 accatcctga cactaagatt catgtgattg atagtttatc tgctggtggt gaagttgacc
    60241 tactcgtaga aaaattgaat gacttgattg accaaggctt gtcttttgaa gaagtggttg
    60301 aagctatcac cgcctatcaa gaaaaaacta agttgctctt tgtcctagcc aaagtcgata
    60361 acttggtgaa gaacggccgt ttgagcaagc ttatcggtac ggtcgttggc cttctcaaca
    60421 ttcgtatggt cggaaaagct agtgaaactg gaactctcga attgctacaa aaagcaaggg
    60481 gatcaaagaa atcagttcaa gctgcctatg atgagttagt aaaagctgga tatgctggtg
    60541 gccgtattgt catggcccaa cgcaataacg aaaaatgttg tcaacagctc tcagagcgaa
    60601 tccgtgaaac cttcccacaa gcggatatta aaattctacc aacctctggt ctctgcagtt
    60661 tctatgcaga agagggcggt ttgctgatgg gctatgaaat tgattaattg cattcttgac
    60721 aagaggtgac catcatctct tgtttttttg tggtacaata gagctatgaa acattttgat
    60781 actattgtca tcggtggggg acctgctggt atgatggcta cgatttccag tagcttttat
    60841 ggacagaaaa ccctcctcat cgaaaaaaat cggaaacttg gaaaaaaatt agctgggact
    60901 ggtgggggac gttgcaatgt gaccaacaat ggtagcttag acaacctgct agctggaatt
    60961 cctggaaacg gacgctttct ttacagtgtt ttctcccagt tcgataatca tgacatcatc
    61021 aactttttta cagaaaatgg tgttaaactt aaggtcgaag accacggacg cgtctttcca
    61081 gccagtgaca agtctcggac tattatcgaa gctttggaaa agaaaatcac cgaactaggt
    61141 ggtcaagttg ctactcaaat agaaatcgtt tctgttaaaa aagtagataa ccagtttgtc
    61201 cttaagtcag cggatcaaac cttcacttgt gagaaactca ttgtcacaac aggtggtaag
    61261 tcttatcctt cgactggttc gactggtttt ggtcacgaga ttgctcgcca ttttaagcat
    61321 accatcaccg atcttgaggc tgctgaaagt cctttattaa cagattttcc acataaagcc
    61381 ttacaaggga tttctttgga cgatgtgacc ctaagttatg gtaagcatgt catcactcat
    61441 gatttactct ttacccactt tggtttgtca ggtcctgctg ccctacgcat gtctagcttt
    61501 gtcaaaggtg gggaggttct ctcactcgat gttttgcctc aactttctga gaaggacttg
    61561 gttacatttc tagaaaaaaa tcgggaaaaa tccttgaaaa acgctttaaa aaccttgtta
    61621 ccagaacgct tggccgaatt ttttgtacaa ggatatcctg aaaaagtcaa acagctgact
    61681 gaaaaggaac gagaacagct tgtccagtcc attaaagaac ttaaaattcc tgtaactgga
    61741 aaaatgtccc ttgcaaagtc ctttgttacc aagggtggag tcagtctcaa ggaaatcaat
    61801 cctaaaaccc ttgaaagtaa gctggtacct ggcctccact ttgcaggcga agttatggat
    61861 atcaatgccc acacgggtgg ctttaacatc acttctgccc tctgtaccgg ctgggtggcg
    61921 ggaagtctgc attatgatta aagattaaag gttgtgaaga atgaacagaa gagaggcagt
    61981 cgaatttgtt aacatgtgta tgattaaaaa cggagataag gtcctggtcc aagaccgagt
    62041 tagtcccgac tggcctggca ttacttttcc tggtggtcat gttgaacgtg gcgaatcctt
    62101 tgtcgatgct gtcattcgtg aagtgaaaga agaaactggt ctgatcattt ccaaacccca
    62161 actctgtggt atcaaaaact ggtatgacga caaggattat cgttatgtcg tcctttttta
    62221 caagacagaa cactttactg gtgaactcca gtcttcagac gaagggaaag tttggtggga
    62281 ggactttgaa aatctttctc atctaaaact tgcaaccgat gatatgtctg atatgcttcg
    62341 tgtgtttcta gaagaagatc tcagtgaatt cttttactac aaaaacggtg acgactggct
    62401 ttatgatttg aagtaaaaaa taggctagga aattcctagc ctattttttc tgcatttgtc
    62461 tgaacgtaag ctgcaatggc atctgacgtg tactgggctg ttccagaacc aaacttgtca
    62521 atgttttcct taaactctgg gttgtagacg taacctttac cgatatgacc gaatacctca
    62581 atagagcagt caaatccata agtgcgaatg gcttgcaaga gcttggctgc ttgctcttgg
    62641 ttttcggttg ctgttgcagg taaaccaact tgaagatttt gtgccaaagt ttgaaagact
    62701 tggttaaagg cggccgtagc ctcgtcttcg tgaccttttt ggcgttcgag cgcttgtccc
    62761 atgacttctt gaccatattt ctctaccgct tcttggtggt atttttgatt gtcttgatag
    62821 ctaaatcccg tgaatttttc ctcaatggtc atttttcttt ctcctttttg ttcttgaata
    62881 gttttttgca aggtggaaat caaggtatcc agatgttgcc tttcgcgagt tagatagtcc
    62941 aactgcctag ttaaatgggg caataaatct gtcctttctt cctttaacag ctctgctatt
    63001 ttctctaaag aaaagcctag atatttgtag taaagaatga cctgaaggcg ttccaaatcc
    63061 tcttgactat aggttcgata gccgttttcc gactttaagg ggaccaagag tcctatcttg
    63121 tcatagtgat gcagggcagg gtcttgacag agacacctga aagctgcgca gcttctttta
    63181 tatggtacat gatttcctcc ttacaatgat agtataaccc ctccccttag gtcagagtca
    63241 agcgtttttg agaaaggtgt gaaaacttga aaatggtttt cttgacagac cttagaaaac
    63301 atgataggat agtcaagtct atcaatcaaa cagaaggcta tttgacaact caagtagctt
    63361 tttcttattt tgaaaaagga gatcagagtt taactatgtc agaaaaatca caatgggggt
    63421 cgaaacttgg ttttattcta gcatctgctg gctcggccat cgggcttggt tccgtttgga
    63481 agtttcccta catgactgct gctaatggcg gtggaggctt tttactaatc tttctcattt
    63541 ccactatttt aatcggtttc cctctcctgc tggctgagtt tgcccttggc cgtagtgctg
    63601 gcgtttccgc tatcaaaacc tttggaaaac tgggcaagaa taacaagtac aactttatcg
    63661 gttggattgg cgcctttgcc ctctttatcc tcttatcttt ttacagtgtt atcggaggat
    63721 ggattctagt ctatctaggt attgagtttg ggaaattgtt ccaacttggt ggaacgggtg
    63781 attatgctca gttatttact tcaatcattt caaatccagc cattgcccta gtagctcaag
    63841 cagcctttat cttattaaat atctttatta tatcacgtgg ggttcaaaaa gggattgaaa
    63901 gagcttcgaa agtcatgatg cccctgctct ttatcatctt tgtttttatc atcggtcgct
    63961 ctctcagttt gccaaatgcc atggaagggg ttctttactt cctcaaacca gacttttcaa
    64021 aactgactag cgctggtctc ctctatgctc tgggacaatc tttctttgcc ctctcactag
    64081 gggttacagt catgttgacc tatgcttctt acttggacaa gaaaaccaat ctagtccagt
    64141 caggaatctc catcgtagcc atgaatatct cgatatccat catggcaggt ctagccattt
    64201 tccaagctcg atcccccttc aatatccagt ctgaaggggg acccagcctg ctctttatcg
    64261 tcttgcctca actctttgac aagatgcctt ttggaaccat tttctacgtc ctcttcctct
    64321 ttgcgactgt cacttcttct gtcgtgatgc tggagatcaa tgtgggcaat atcaccaacc
    64381 aggataacag caaacgtgcc aaatggagtg ttattttagg aattttgacc tttgtctttg
    64441 gcattccttc agccctatct tacggtgtca tggcggatgt tcacattttt ggtaagacct
    64501 tctttgacgc tatggacttc ttggtttcca atctcctcat gccatttgga gctctctacc
    64561 tttcactttt tacaggctat atctttaaaa aggctcttgc aatggaggaa ctccatctcg
    64621 atgaaagagc atggaaacaa ggactgttcc aagtctggct cttccttctt cgtttcttcg
    64681 ttccgtcatt ccaatcatca tcattgtggt cttcattgcc caatttatgt aatcaaaaag
    64741 gacttgagta gtgaactcag gccctttctt tttatggatg gctaacaatc aattccaaac
    64801 cttgcccttc cagagtccaa gcttcaacat cacttggtag gataaagtgg ctgccttttt
    64861 gaattggata atttttcccg tcaacagtta gctgaccttg accagctaag acactcaata
    64921 agctgtagtc agctgtcttt tcaaagtcaa cttttccagt aatttcccac ttgtaaactg
    64981 cgaagaaatc attagataca aggagagtgg aacgcaaatc atctgcttta acagttacag
    65041 gacggctatt tgctggctca ccaatgttca agacatcgat ggatttttca agatgaagtt
    65101 cacgcaagtt gcctttgtca tccttgcggt caaagtcata gacgcgatag gtggtatcgc
    65161 tagactgctg ggtttcaagg atgaagatac ccgccccgat agcgtgcata gtcccgcttg
    65221 gtacatagaa gaaatctcca gccttaacag ggactttggt caacaagtca tcccagttct
    65281 tgtcctcgat ttgctggcgg agttcttctt ttgacttggc attgtgaccg tagataatct
    65341 ctgaaccttc atccgctgcg ataatgtacc agcattctgt ttttccgagt tcgccttcat
    65401 gctcgagtcc ataagcatcg tctgggtgaa cttggacact gagccagtcg ttggcatcga
    65461 ggatcttggt caaaagtgga aatacaggtt ctggacgatt gccaaataat tcacggtgtt
    65521 ccgcatacaa agtagcaaga tctgttccct cgtaacgacc attggcaact ttagagactc
    65581 catttggatg ggctgagatg gcccaatatt ctccgatttt ttcacttggg atgtcgtagc
    65641 caaactcatc acgtagcttg gctccacccc agattttttc ttgcataact gattgtaaaa
    65701 ataatggttc tgacatgtcg atctcctgtc tgatttttct cccctcatta tagcaaaaaa
    65761 agagttcgaa ttgaactctt ttttacatct tataaagcag ggagaagatt ttataaaaat
    65821 agtaaacaaa tgtgctctac tcgatgctta caccattgct ataaatgaca tgtaccaata
    65881 gaaggacttc ttcttgctac gtttgagagt cccatttcct acattatctc gatctacata
    65941 gataaagcca tagcgcttat tcatttcccc tgtgccagct gaaaccgaat cgatacagcc
    66001 ccatgtcgta taaccaagca agtcaacccc gtctttgtaa atggcatctc gcatggcctt
    66061 gatgtgggcc tctaagtaag taatccgata gtcatctgct acataaccat tctcatccgg
    66121 tgtatccata gcaccgagtc cattttctac gataatacta tactaaaatc aaaaagcatt
    66181 atataatagt gatatgaaat caactaaaga agaaatccaa accatcaaaa cactttttaa
    66241 agactctcgt acagctaaat atcataaacg ccttcaaatc gttctatagt aaaacgaaac
    66301 aagaacaggg caaatcgatc aggacagtca aatcgatttc taacaatgtt ttagaagtag
    66361 aggtgtacta ttctagtttc aatttattat atttcgtctg atgggcaaat cttataaaga
    66421 gattatagaa cttttatagt agtttgaaat aagatgtgaa caactctatc aggaaagtca
    66481 aactaattta tagaaatatt ttagcagcta aggtgtactg ttatagattc aatacact
//
LOCUS       DAVKJJ010000004        66124 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000004 DAVKJJ010000000
VERSION     DAVKJJ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 66124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66124
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.004"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            16..86
                     /locus_tag="VW144_000229"
     tRNA            16..86
                     /locus_tag="VW144_000229"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48..50,aa:Gly,seq:tcc)
     gene            122..195
                     /locus_tag="VW144_000230"
     tRNA            122..195
                     /locus_tag="VW144_000230"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:156..158,aa:Ile,seq:gat)
     gene            202..289
                     /locus_tag="VW144_000231"
     tRNA            202..289
                     /locus_tag="VW144_000231"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:236..238,aa:Ser,seq:gct)
     gene            complement(415..1350)
                     /locus_tag="VW144_000232"
     CDS             complement(415..1350)
                     /locus_tag="VW144_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEU9599735.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLIQCDNGKEIWLNSGEISLNSWK"
     gene            complement(1347..2207)
                     /locus_tag="VW144_000233"
     CDS             complement(1347..2207)
                     /locus_tag="VW144_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9599736.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELSQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYIRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            2315..4477
                     /locus_tag="VW144_000234"
     CDS             2315..4477
                     /locus_tag="VW144_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEU9599737.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQIEKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRRHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHDIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMMRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGLVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTVSNEAAVQFNYENQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRRL"
     gene            4512..5771
                     /locus_tag="VW144_000235"
     CDS             4512..5771
                     /locus_tag="VW144_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9599738.1"
                     /translation="MEWYKKIGLLATTGLALVGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            5785..6651
                     /locus_tag="VW144_000236"
     CDS             5785..6651
                     /locus_tag="VW144_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9599739.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIVMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            6665..7498
                     /locus_tag="VW144_000237"
     CDS             6665..7498
                     /locus_tag="VW144_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9599740.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            7809..9251
                     /gene="gtfA"
                     /locus_tag="VW144_000238"
     CDS             7809..9251
                     /gene="gtfA"
                     /locus_tag="VW144_000238"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEU9599741.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKALFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVVEIDLQNQTYRVIENGVEVSF"
     gene            9389..10582
                     /locus_tag="VW144_000239"
     CDS             9389..10582
                     /locus_tag="VW144_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU9599742.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            11268..13247
                     /locus_tag="VW144_000240"
     CDS             11268..13247
                     /locus_tag="VW144_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9599743.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAARETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            13314..14810
                     /locus_tag="VW144_000241"
     CDS             13314..14810
                     /locus_tag="VW144_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9599744.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGTLTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPVTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            14810..15736
                     /locus_tag="VW144_000242"
     CDS             14810..15736
                     /locus_tag="VW144_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9599745.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            15745..16812
                     /locus_tag="VW144_000243"
     CDS             15745..16812
                     /locus_tag="VW144_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9599746.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYAPCHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            16823..17749
                     /locus_tag="VW144_000244"
     CDS             16823..17749
                     /locus_tag="VW144_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9599747.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQSIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            18367..19176
                     /locus_tag="VW144_000245"
                     /pseudo
     CDS             18367..19176
                     /locus_tag="VW144_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813348.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            19188..20065
                     /locus_tag="VW144_000246"
                     /pseudo
     CDS             19188..20065
                     /locus_tag="VW144_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            20078..20881
                     /locus_tag="VW144_000247"
     CDS             20078..20881
                     /locus_tag="VW144_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9599748.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFIYVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKNLSVRKRAKDIARVFQYPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMAALVKPKLLLLDEHTAALDPKTSQMVMDLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(20914..21624)
                     /gene="treR"
                     /locus_tag="VW144_000248"
     CDS             complement(20914..21624)
                     /gene="treR"
                     /locus_tag="VW144_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEU9599749.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYIVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            21809..23776
                     /gene="treP"
                     /locus_tag="VW144_000249"
     CDS             21809..23776
                     /gene="treP"
                     /locus_tag="VW144_000249"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEU9599750.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLRDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEATKSAAKSNQNVV
                     QGVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQVQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            23954..25579
                     /gene="treC"
                     /locus_tag="VW144_000250"
     CDS             23954..25579
                     /gene="treC"
                     /locus_tag="VW144_000250"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEU9599751.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWTPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKYINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            25835..26083
                     /locus_tag="VW144_000251"
     CDS             25835..26083
                     /locus_tag="VW144_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEU9599752.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            26290..27084
                     /gene="racE"
                     /locus_tag="VW144_000252"
     CDS             26290..27084
                     /gene="racE"
                     /locus_tag="VW144_000252"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU9599753.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            27081..28052
                     /locus_tag="VW144_000253"
     CDS             27081..28052
                     /locus_tag="VW144_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEU9599754.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNAYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            28028..28549
                     /locus_tag="VW144_000254"
     CDS             28028..28549
                     /locus_tag="VW144_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU9599755.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWMEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            28546..29007
                     /locus_tag="VW144_000255"
     CDS             28546..29007
                     /locus_tag="VW144_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9599756.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            28998..29738
                     /gene="xerD"
                     /locus_tag="VW144_000256"
     CDS             28998..29738
                     /gene="xerD"
                     /locus_tag="VW144_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU9599757.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            29738..30466
                     /locus_tag="VW144_000257"
     CDS             29738..30466
                     /locus_tag="VW144_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEU9599758.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            30485..31054
                     /locus_tag="VW144_000258"
     CDS             30485..31054
                     /locus_tag="VW144_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEU9599759.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            31041..31763
                     /locus_tag="VW144_000259"
     CDS             31041..31763
                     /locus_tag="VW144_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9599760.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            31763..32005
                     /gene="yidD"
                     /locus_tag="VW144_000260"
     CDS             31763..32005
                     /gene="yidD"
                     /locus_tag="VW144_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU9599761.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(32230..33195)
                     /locus_tag="VW144_000261"
     CDS             complement(32230..33195)
                     /locus_tag="VW144_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9599762.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIIGMPTKTIPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSIKANIES
                     LASAFGETSIQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(33291..34043)
                     /locus_tag="VW144_000262"
     CDS             complement(33291..34043)
                     /locus_tag="VW144_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9599763.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            complement(34040..34996)
                     /locus_tag="VW144_000263"
     CDS             complement(34040..34996)
                     /locus_tag="VW144_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU9599764.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSFFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            complement(34986..35945)
                     /locus_tag="VW144_000264"
     CDS             complement(34986..35945)
                     /locus_tag="VW144_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9599765.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(36242..36352)
                     /locus_tag="VW144_000265"
     CDS             complement(36242..36352)
                     /locus_tag="VW144_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HEU9599766.1"
                     /translation="MNYLFQEDSSSQIFNLETKKGYTIKEIFKTAEELLN"
     gene            complement(36368..37069)
                     /locus_tag="VW144_000266"
     CDS             complement(36368..37069)
                     /locus_tag="VW144_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HEU9599767.1"
                     /translation="MTKTILVTGETGYIGSHTVKALLNAGYQVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKSYLEENQIDAALHCAGETVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNAAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            37469..38533
                     /gene="pepA"
                     /locus_tag="VW144_000267"
     CDS             37469..38533
                     /gene="pepA"
                     /locus_tag="VW144_000267"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEU9599768.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALMKKLDRSTVDLIKHY"
     gene            38555..38851
                     /locus_tag="VW144_000268"
     CDS             38555..38851
                     /locus_tag="VW144_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9599769.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLASFIKREESKYRGTATSALMYEELFKML"
     gene            39533..39976
                     /locus_tag="VW144_000269"
     CDS             39533..39976
                     /locus_tag="VW144_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9599770.1"
                     /translation="MRDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            39980..40744
                     /locus_tag="VW144_000270"
     CDS             39980..40744
                     /locus_tag="VW144_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU9599771.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVVDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHDIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            40759..41487
                     /locus_tag="VW144_000271"
     CDS             40759..41487
                     /locus_tag="VW144_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9599772.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKAPATDFVADLFGGSVHD"
     gene            41480..43000
                     /locus_tag="VW144_000272"
     CDS             41480..43000
                     /locus_tag="VW144_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEU9599773.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPNLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLAALIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLVQKEKENLVIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGKTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMEPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            43345..44151
                     /locus_tag="VW144_000273"
     CDS             43345..44151
                     /locus_tag="VW144_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEU9599774.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(44318..44854)
                     /locus_tag="VW144_000274"
     CDS             complement(44318..44854)
                     /locus_tag="VW144_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9599775.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNLPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            44987..45877
                     /locus_tag="VW144_000275"
     CDS             44987..45877
                     /locus_tag="VW144_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU9599776.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            46121..46474
                     /locus_tag="VW144_000276"
     CDS             46121..46474
                     /locus_tag="VW144_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU9599777.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            46475..47512
                     /locus_tag="VW144_000277"
     CDS             46475..47512
                     /locus_tag="VW144_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9599778.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLQSLLTLADVMPTCYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(47558..48565)
                     /gene="galR"
                     /locus_tag="VW144_000278"
     CDS             complement(47558..48565)
                     /gene="galR"
                     /locus_tag="VW144_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEU9599779.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGR
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            48730..49908
                     /locus_tag="VW144_000279"
     CDS             48730..49908
                     /locus_tag="VW144_000279"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEU9599780.1"
                     /translation="MAQHLTTEVLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            49927..51408
                     /locus_tag="VW144_000280"
     CDS             49927..51408
                     /locus_tag="VW144_000280"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU9599781.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLEMIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSRKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDRTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGILLD"
     gene            51478..51882
                     /locus_tag="VW144_000281"
     CDS             51478..51882
                     /locus_tag="VW144_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU9599782.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEDRQVRI"
     gene            52039..52893
                     /locus_tag="VW144_000282"
     CDS             52039..52893
                     /locus_tag="VW144_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEU9599783.1"
                     /translation="MKIKEIKKVTLQPFTKWTGGKRQLLPVIRELIPKTYNRYFEPFV
                     GGGALFFDLAPKDAVINDFNAELINCYQQIKDNPQELIEILKVHQEYNSKEYYLDLRS
                     ADRDERIDMMSEVQRAARILYMLRVNFNGLYRVNSKNQFNVPYGRYKNPKIVDEELIS
                     AISVYINNNQLEIKVGDFEKAIVDVRTGDFVYFDPPYIPLSETSAFTSYTHEGFSFAD
                     QVRLRDAFKRLSDTGAYVMLSNSSSALVEELYKDFNIHYVEATRTNGAKSSSRGKISE
                     IIVTNYEK"
     gene            52919..53689
                     /locus_tag="VW144_000283"
     CDS             52919..53689
                     /locus_tag="VW144_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU9599784.1"
                     /translation="MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNG
                     GISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYS
                     VGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLM
                     KELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGS
                     GTTGVVAKRLGRRFIGIDAEKEYLKIARKRLEAENETN"
     gene            53676..54542
                     /locus_tag="VW144_000284"
     CDS             53676..54542
                     /locus_tag="VW144_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnII"
                     /protein_id="HEU9599785.1"
                     /translation="MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIM
                     NSLIGSKNIQEDFLDLYQNYPEILKVVPLLIAKRLRDTIIVKDPIKDFYFDFSKRNYS
                     IEEYTMFLEKSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIVQSYLE
                     AEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLIEVNFY
                     SGSGSKLNETARSYKMIAEETKAIPNVEFMWITDGQGWYKAKNNLRETFDILPFLYNI
                     NDLEHNILKNLK"
     gene            complement(54629..55891)
                     /locus_tag="VW144_000285"
     CDS             complement(54629..55891)
                     /locus_tag="VW144_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEU9599786.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIAMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(55891..56472)
                     /locus_tag="VW144_000286"
     CDS             complement(55891..56472)
                     /locus_tag="VW144_000286"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEU9599787.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     regulatory      complement(56872..56967)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(57350..57478)
                     /locus_tag="VW144_000287"
     CDS             complement(57350..57478)
                     /locus_tag="VW144_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEU9599788.1"
                     /translation="MKANYPIYRPLTKRKFRVSDPYIYPHKFAVLDPNGYFLRFSE"
     gene            complement(57485..57736)
                     /locus_tag="VW144_000288"
     CDS             complement(57485..57736)
                     /locus_tag="VW144_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU9599789.1"
                     /translation="MDYNAVIPELLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLSL
                     EECQLMLEEGTKDQLAELTYPFGRGVNLSFGIKDVSKLY"
     gene            complement(57748..58575)
                     /gene="xth"
                     /locus_tag="VW144_000289"
     CDS             complement(57748..58575)
                     /gene="xth"
                     /locus_tag="VW144_000289"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU9599790.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            58709..59164
                     /locus_tag="VW144_000290"
                     /pseudo
     CDS             58709..59164
                     /locus_tag="VW144_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            59236..59613
                     /locus_tag="VW144_000291"
     CDS             59236..59613
                     /locus_tag="VW144_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEU9599791.1"
                     /translation="MKRLIDGKPTLLIKNGNIDPEACRSVGLSASDVALKLRSQEIFQ
                     MKQVKRAVQEQNGQLIVVQMGDENPKYPVVTDGVIQVDVLESIGRSEEWLLDNLSKQG
                     HDNVANIFIAEYDKGAVTVVTYE"
     gene            59819..61564
                     /locus_tag="VW144_000292"
     CDS             59819..61564
                     /locus_tag="VW144_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9599792.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYTTSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLLAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVNKGTI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILNPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGRHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            61554..63305
                     /locus_tag="VW144_000293"
     CDS             61554..63305
                     /locus_tag="VW144_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9599793.1"
                     /translation="MQNKQEQWVVLNRLMSYLKPYRLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLAYANRSVALDALFLRPAMSLLKLLGYAVLMTYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLTLGG
                     TYHKMYSLQAGAMAYTL"
     gene            63478..64170
                     /locus_tag="VW144_000294"
     CDS             63478..64170
                     /locus_tag="VW144_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9599794.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFLGDI
                     KIMFQTVFEVLK"
     gene            64213..65439
                     /locus_tag="VW144_000295"
     CDS             64213..65439
                     /locus_tag="VW144_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEU9599795.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERCLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
BASE COUNT        19183 a        12228 c        14837 g        19876 t
ORIGIN      
        1 attgatagta tttatgcggg tgtagtttag tggtaaaact acagccttcc aagctgttgt
       61 cgcgagttcg attctcgtca cccgctttga actttgttca aattaccaag ttttttaact
      121 tgggcgcgta gctcaggtgg ttagagcgca cgcctgataa gcgtgaggtc ggtggttcga
      181 gtccactcgt gcccattaat tggagaatta ctcaagaggc tgaagaggac ggtttgctaa
      241 atcgttaggt cgggtaactg gcgcaagggt tcgaatccct tattctccgt tttaatgaga
      301 gtttgtaact ctcttttttg tttttagaga caataaaact tcagatccgt aaaacttaat
      361 tgaattacta atctgaagtt atttttatat cgtttatatt ataattgtgt tattttactt
      421 ccaactattg agagaaattt cgccactatt tagccagatt tcttttccgt tatcacattg
      481 aattaaaagt tttccatttt ctgagatgtc tttagcaagt cccttgtagt ctttttgctc
      541 tagtgtgaaa gtgacttctt ttcctagaat gaatgactgt tttttgtata ggtataatag
      601 ctcttctgct ggtgtttcga agaaagcacg ccagatttct atgatcaatt cattccttgt
      661 tataggagct gtagctttaa ataagctggc agctttttct tttaattcct gagggaagtc
      721 tttaatagtg aaattgatac ctactccaat aatgatatct gtgactaagc cagtttctac
      781 agaggtcatt gcttcagtaa ggattcctcc aattttatga ttgtttagat agatatcatt
      841 gacccatttt atgtcgacat ctattaaagt taggttctta atagctttgt agacagctcc
      901 agctacaagt agtgtgtagg atggtaattt gtcatagggg agatttggtt taagatggag
      961 tgtcatataa ataccacctt gtggtgagta gaaggaacgt tgaaaacggc ctcggcctgc
     1021 tgtttgatag gaagctagat agagggtatt tgcttcatgg cctaaatcaa ttgcttcttt
     1081 tgcatctagt tgtgttgatt ttgtttcggg tttaaagctg actttaattg gaagattttc
     1141 ttctagaatc tctggaagaa taaggtcacc attcatcaat ttatatcctc tatttttgat
     1201 actatcaatt tcaatgcctt cttgttctag tcgcttgatg gctttccaaa ttgatgttcg
     1261 gcttagggat agtttttctg cgattttttc tccgctgata tagtcggttt ctttagatag
     1321 gatttggtag acagcttggt aggatttcat aatgttgcct ttctcactag ttggtattga
     1381 gagtattctt ttcttgtatg acttggagtc tgattaaagt attgtttata agctttcgaa
     1441 aaatggagtg gatctgaaaa acctaccgag tatgcaatta ccttgatgga ctcttgggta
     1501 ttttcgagaa gttgtctagc tcggtgcatt cgaatgtaga gtaggtattc tttgggtgat
     1561 aaggtattaa attctttgaa tacgcttgat aagtagcttc tgtgaacgga tagttctttt
     1621 gctaaatctt gaattgtaag tgattgagga tagtggctat caattaatcg tttgcattca
     1681 agatagagtt ggtgggttga tgaaatattc ttttttttct gattgggagc aatagttccc
     1741 agatgaaaca tcagttcatg aagttgtccc atgatatgga gttgagataa ttcacttgat
     1801 tttgtaatct gagcgaagcg gacaatgtct gagatgagtt ttgcagtagt ctgggtatga
     1861 caagtttcag attggatgag ataggattga tcagaaattt gagaaagagc aaaataatca
     1921 ggggatttcc ctccagtgat tcctaaccag tagtaggccc aaggttcttt actatctgct
     1981 tgataaaagg ttagttcctc tggttttaat agaaagaaat ctccttcttt taaatcaaca
     2041 attttaccct tgtaatgaaa ttttccttgt cctttagtaa tgtaatgtag gacgtatgta
     2101 tcacgaatgg ctggaccaaa agagtaatta ggtgtgcatt cctcatatcc ataaaagctt
     2161 agggcaaggt caattgttcc agtctggtat tctgaaaaaa ctagcatgtg ctacctccta
     2221 cctaacattt taccatattc ttgagacatt tttctatttt ggaaagcgat ttcaggtgat
     2281 aaaatataat caataaagtg atgaggtgaa gtaaatggga gttaggatag agaataatct
     2341 attttatgtt gagagtaaaa atctaagttt gattattgaa aatcgaaatg gctacttact
     2401 tttgaaacat ttaggaaaga ctattaagaa ctataaaggt tccaatagtg tttatgaacg
     2461 agaccatgcc ttttcaggaa atccaacggc tactaatcga acctttagtt tagatactca
     2521 gcgacagatt tttggacaac atggcttagg agattttagg aaaccaacca tacaggttca
     2581 gcatagtgta actgaagtaa cagactttcg atttgtagaa gcaaagattt taaaaggtca
     2641 gaatggtcca cagggcttac cttctccaca tagcatggac gatacagaga ctcttgtctt
     2701 aatgttagaa gattctaagg ctcaacttag tctgactttg tattatacta cttttaataa
     2761 tgatgcgact attgctagct acagtaaatt agataataat agtaatcagg aagttgtcat
     2821 ccataaagat ttttctttta tggctgattt tccagctgca gattacgaaa tagtaactct
     2881 gcagggtgct tatgctcgtg aaaagactgt tagacgtcaa caggtagaac aaggaatctt
     2941 ttcgattagc tcaaaccgag gtgcttctgg tcatgctcaa acaccagctc ttctactatg
     3001 cgaacaagga gtcacagagg atgctgggaa tgtgtttgct attcaactaa tgtatagtgg
     3061 caactttgaa gcttttgttc aaaaaaatca attgaatgaa gttcgggtgg ctattggcat
     3121 taatccagaa aacttttctt ggaagttagc tcctgaggaa tactttgaaa caccggtagc
     3181 tttagtgacc cattcagatc agggattaac tggtattagt catgaaagtc agaattttgt
     3241 actgaagcac attatgctaa gtgaattttc taaaaaagaa cgtccaattc taatcaataa
     3301 ctgggaagct acttactttg actttcagat agaaaaactg ttagagttag cagatgaagc
     3361 taagaaagtt ggcattgaac tttttgtatt agatgatggt tggtttggca atcgttttga
     3421 tgataatcgt gctttaggtg attgggttgt taatgaggaa aaactgggtg gaagtctaga
     3481 aagtctgatt tcagctatcc atgaaagagg tttgcagttt ggactttggt tagaacccga
     3541 aatgatttct gtagatagtg atttgtatcg tcgacatcct gactgggcta ttcaggttcc
     3601 tgactatgag catacttatt ctcggaatca attagtactt aatcttgcca atcctcaggt
     3661 agtagaatac ttgaaaagtg tcttagatca actcctatct tatcatgata ttgattacat
     3721 taaatgggat atgaaccgca atatcactaa gctagggaat ggattaactt atctagagac
     3781 acagatgcaa tctcatcagt acatgctggg gctttacgaa ctcgtttctt atctgacaga
     3841 gaagcacagc catattctct ttgagtcctg ctctggtggt ggtggacgaa atgatcttgg
     3901 tatgatgcgc tatttcccac aagtttgggc tagtgataat actgatgcca ttgcacgttt
     3961 accaattcaa tacggttcat cctatctcta tccaaccatt tctatggggg ctcatgtgtc
     4021 agcagtaccg aatcatcaga tgggacgaat gacaccatta gaaacacgtg gccttgtagc
     4081 aatgatggga aatttgggct atgaacttga tttgacaaat ttatcagatg aagagaaagc
     4141 tacgattgct aatcaggtga acttgtataa agaattacga ccagtagttc agttaggaca
     4201 gcagtataga ctaattaatc ctgatactgt atccaatgaa gcagctgtac aatttaatta
     4261 cgaaaatcaa acgattgtaa cctacgttcg cgttttatct gttgtagaga ccatggaaac
     4321 aactttaaaa ttaaaagatt tggatgaaga gggactatat aaattacagg aaaatggcga
     4381 agtttactca ggtgcagaac taatgtatgc aggcctaact gttatcttat cccaaggaga
     4441 ttttttgagt agacagtata tttttagaag actatgataa gggtgtataa atttataaag
     4501 gaggctttct aatggaatgg tataaaaaaa tcggacttct tgcaactaca ggtttagctt
     4561 tggttgggct cggcgcttgc tccaactatg gtaaatctgc ggatggcaca gtgaccatcg
     4621 agtatttcaa ccagaaaaaa gaaatgacca aaaccttgga agaaatcact cgtgattttg
     4681 agaaggaaaa ccctaagatc aaggtcaaag tcgtcaatgt accaaatgct ggtgaagtat
     4741 tgaagacacg cgttctcgca ggagatgtgc ctgatgtggt caatatttac ccacagtcca
     4801 tcgaactgca agaatgggca aaagcaggtg tctttgaaga tttgagcaac aaagactacc
     4861 tgaaacgcgt gaaaaatggc tacgctgaaa aatatgctgt aaacgaaaaa gtttacaacg
     4921 ttccttttac agctaatgct tatggaattt actacaacaa agataaattc gaagaactgg
     4981 gcttgaaggt tcctgaaacc tgggatgaat ttgaacagtt agtcaaagat atcgttgcta
     5041 aaggacaaac accatttgga attgcaggtg cagatgcttg gacactcaat ggttacaatc
     5101 aattagcctt tgcgacagca acaggtggag gaaaagaagc aaatcaatac cttcgttatt
     5161 ctcaaccaaa tgccattaaa ttgtcggatc cgattatgaa agatgatatc aaggtcatgg
     5221 acatccttcg catcaatggc tctaagcaaa agaactggga aggtgctggc tataccgatg
     5281 ttatcggagc cttcgcacgt ggggatgtcc tcatgacacc aaatgggtct tgggcgatca
     5341 cagcgattaa tgaacaaaaa ccgaacttta agattgggac cttcatgatt ccaggaaaag
     5401 aaaaaggaca aagcttaacc gttggtgcgg gagacttggc atggtctatc tcagccacca
     5461 ccaaacatcc aaaagaagcc aatgcctttg tggaatatat gacccgtcca gaagtcatgc
     5521 aaaaatacta cgatgtggac ggatctccaa cagcgatcga aggggtcaaa caagcaggag
     5581 aagattcacc gcttgctggt atgaccgaat atgcctttac ggatcgtcac ttggtctggt
     5641 tgcaacaata ctggaccagt gaagcagact tccatacctt gaccatgaac tatgtcttga
     5701 ccggtgataa acaaggcatg gtcaatgatt tgaatgcctt ctttaacccg atgaaagcgg
     5761 atgtggatta ggagtagaga gactatgaaa aaagtattac aaaaatattg ggcatgggct
     5821 tttgtggtca tccccctctt gttacaagca attttcttct atgtgccgat gtttcaagga
     5881 gccttttaca gttttaccaa ctggacagga ttgacttaca actacaaatt tgtcggctta
     5941 aacaacttta agctcctctt catggatcca aaattcatga atgcgattgg ctttaccgca
     6001 atcattacga ttgtcatggt ggttggtgag attgcactcg ggatcttcat tgcgcgtgtc
     6061 ttgaactcta aaatcaaagg ccaaaccttc ttccgtgctt ggttcttctt cccagctgtt
     6121 ttatctggtt tgacagtggc tttgatcttc aagcaagtct tcaactacgg tcttccagcg
     6181 attgggaatg cccttcatat tgaatttctc caaaccagtc ttttagggac taagtgggga
     6241 gcaatctttg cggctgtctt tgtccttctt tggcaagggg tggctatgcc catcatcatc
     6301 ttcctagctg gtttgcaatc tattccaact gagattacag aggcagcaag gattgatggt
     6361 gcgactagca agcaagtttt ctggaacatt gaattgcctt acttgctacc aagtgtctct
     6421 atagtcttta tcctagccct aaaaggtggg ctgactgcct ttgaccaagt ctttgccatg
     6481 accggtggtg gtccaaacaa tgccacaacc tcacttgggc tcttggttta taactatgcc
     6541 tttaaaaaca accaattcgg ttatgccaat gccattgccg taatcttgtt cttcttaatt
     6601 gtagtgattt cgatcatcca attgagagta tctaagaaat ttgaaattta agaggagaag
     6661 catgatgaaa caagatgaaa gaaaagccct gattggcaaa tacattctat tgattctagg
     6721 atcggttctg attttagtgc cgctccttgc taccctcttt agttccttca aacccactaa
     6781 ggatattgta gataatttct ttggctttcc aaccaacttc acatgggaca actttagccg
     6841 tctcttagct gatgggattg gaggctatta ttggaactct gtcgtcatca ctgtcttatc
     6901 tttacttgca gtaatgatct ttatacctat ggcagcctac tccatcgctc gcaatatgag
     6961 taaaagaaaa gcctttacca tcatgtatac cctcttaatc ctcggaatct tcgtaccttt
     7021 ccaagtcatc atgattccga ttacggttat gatgagtaaa ctcggtttgg ctaatacctt
     7081 tggtttgatc ttactctact tgacctatgc gattccacag accctctttc tctatgttgg
     7141 ctatatcaaa atctcgattc cagaaagtct ggatgaagca gcagagatcg atggggctaa
     7201 tcaatttaca acctatttcc gcatcatctt cccaatgatg aaaccgatgc atgcgacaac
     7261 catgatcatc aatgcccttt ggttctggaa tgacttcatg ttgccactcc ttgtcttgaa
     7321 ccgggattcc aaaatgtgga ctctgccttt gttccaatac aactacgcag gccaatattt
     7381 caacgactac ggaccaagct ttgcctctta cgtggtcggc attatcagta tcaccattgt
     7441 ctatctcttc ttccaacgcc atatcatttc aggaatgagc aacggggcag tgaagtaata
     7501 ctcaatgaaa atcaaagagc aaactaggaa gctagccgca ggctgtactt gagtacggta
     7561 aggtgaagct gacgtggttt gaagagattt tcgaatagta taagaagagc tcatggaatc
     7621 tatagtcttt gacacagagg aagagttcag cttgttgata attctatgta gaaaacaaga
     7681 aagtagtact ttgtttatca cagtctatat tagtacgagt ttgatgataa atagagattt
     7741 aaaatgaatt ttatttatga agccagatca tatgatactt ggcttcaatt agaaaaagga
     7801 gagtaatgat gccaattcaa aataaaacca tgttgattac ctattctgat agccttggaa
     7861 ataatcttaa agacttatat gataatttgg aagagcattt tggagatgct attggaggag
     7921 ttcacctttt accatttttc ccatcaacag gtgatcgtgg atttgcgcca gttgactacg
     7981 acgaagtgga ctcagctttt ggtgattggg aggatgttaa gcgtttaggt gagagatatt
     8041 atcttatgtt tgattttatg attaatcata tttctcgtca atccaagtat tataaggact
     8101 atcaagaaaa acatgaagcc agtgaattta aagctctctt tttaaactgg gataagtttt
     8161 ggccagaaaa ccgtccgaca cagtctgatg tagatttaat ttacaagcgt aaggatcgtg
     8221 caccaaagca agagattgtg tttgaagatg gttcagtgga acatttgtgg aatacctttg
     8281 gtgaggagca gattgatctt gatgtgacca aagaagtaac tatggaattt atccgtaaga
     8341 ccattcagca cttggcaagt aatgggtgtg atttgattcg tctagatgcc tttgcttatg
     8401 cagtgaagaa attggatact aatgatttct ttgtggaacc agatatttgg gatttattgg
     8461 acaaagttcg agatatcgct gctgagtatg ggacagagct tttacctgag attcatgaac
     8521 actattcgat tcagtttaaa atagcagacc atgattacta tgtttatgat tttgctcttc
     8581 caatggtgac actttatact ctttacagtt ccagaacaga gcgtttggct aagtggttaa
     8641 agatgagccc gatgaagcaa tttacgacgc tagataccca tgatgggatt ggagtagtag
     8701 atgtcaagga tatcctgacc gatgaggaga ttgactatgc ttcaaatgaa ctctataagg
     8761 ttggagccaa tgtcaaacgt aagtactcta gtgccgagta taacaactta gatatctacc
     8821 aaatcaattc aacctactat tcagcgcttg gagatgatga tgtcaagtat tttctcgctc
     8881 gtctaattca agcttttgcc ccaggtattc ctcaggttta ctatgtgggt ctattagcag
     8941 gcaagaatga cttgaaatta ttagaagaaa ctaaagaagg tcgaaatatt aatcgtcatt
     9001 actatagcaa cgaggaaata gcaaaagaag tgcaacgacc tgttgtgaag gcccttctca
     9061 atctattttc tttccgtaac cgttcagaag cctttgatct agaagggact actgagatag
     9121 agacaccaac agctcacagc attgtaatca aacgtcaaaa taaagataag tccgtaacag
     9181 cagtagtaga aattgatttg caaaatcaga cttatcgggt aattgagaat ggagttgaag
     9241 tatcattttg aagccttgat atggtggata aaatagggtt tttattttgg aaaacgtttc
     9301 ctttgttttc aaattgctaa aaaagtggta caatatagga tagcttacta ttatctgaat
     9361 cagctgattt ggagagaaag gattcatttt gaaatcaata ggctttattg aaaagctgaa
     9421 ggggttgtct agtaaagagc tgattttatt gggaattatc ctaagtatct ttttaccctt
     9481 ttatcttttt gtagttgtac tctgtttata tattatcagt ttgattttta caggagacat
     9541 gaaaagtatt cttcagaaaa tgggggagca tccgatgctg cttctttttc ttagctatag
     9601 tactgttata tccattcttg cacaaaattg gatgggtctt gtggcttcag taggaatgtt
     9661 tctatttact attttctttt tgcactatca gtcgatttta tcccataaat tctttcgatt
     9721 gattttgcag ttcgtcttgt ttggtagtgt cttgtcagct gcttttgcca gtttagaaca
     9781 tttccaaatt gtgaagaaat ttaactatgc ttttctttca cccaatatgc aggtgtggca
     9841 tcagaaccgg gcagaagtga ccttctttaa tcctaattat tatggaatta tttgttgttt
     9901 ctgtattatg attgctttct atctgtttac aacgaccaag ttgaattggt tgaaagtatt
     9961 ctgtgtgatt gcaggctttg ttaatctctt tggtttgaac tttactcaaa atcgaactgc
    10021 ctttcctgct attatcgctg gagcaattat ctatctcttt acgactatta aaaactggaa
    10081 ggccttttgg cttagtattg gggtcttcgc gattggtttg agtttcctct tttctagtga
    10141 tttgggagtt cgaatgggta ctttagactc ttctatggaa gaacgcattt ctatctggga
    10201 tgctgggatg gccttgttta agcaaaatcc tttttggggt gaagggccat tgacctatat
    10261 gcactcttat cctcggatac atgctcctta tcatgaacat gcccacagtc tttatattga
    10321 tacgattctg agttacggaa ttgtgggtac cattttatta gttttgtctt ctgttgctcc
    10381 tgttcgcttg atgatggata tgagtcagga gtcggggaaa cgtccgatta tcggccttta
    10441 tctatctttc cttacagtgg ttgctgtgca cggaattttt gacttggctc tcttctggat
    10501 tcagtcaggc tttattttct tgctagttat gtgcagtatt ccattggagc atcgaatgtt
    10561 ggtatcggac atgacggatt aagttttata agtttgaaat cttctacctt gggtaggaga
    10621 tttttttata ctatgggcat gtcgtgcatc tgaggtgtaa gtcctctgta gggatccact
    10681 accggtgaat acaatagcgc tccccaagcc tgactgtcgt gaggtagcgg attaaaatgg
    10741 tctggggata gatcgtttta agtctgacgc aggaaataag aattgtcaga gaaagcaata
    10801 gtgaaatcgt aggctatgaa tagaaacgtg atagcgagga gcatatagtg gataaggtag
    10861 cgagaaattt taaagtataa aagatagtct taatctatat atgtaaatca caaaagtaat
    10921 tgaattcgga ataaggttgg tgtgaaaaga catagtaaac tgaatcttga atactagaat
    10981 gatacttcta caatattgtt ataatttaat ttgaattcca aaatcacatt gttccgtccg
    11041 tatttcattt tactgtatgt cttcctaaac tccaaagttt ctgcgtcaaa tttcctattt
    11101 tcactgtagt ctttgacact tcgaacgaca atttgaatca tatattcaaa aaaaatgaaa
    11161 aatttgtgcc aacaagccta aatattctga aaatttgttt gatttttaat ggataatgtg
    11221 atataatggt tgtcaatcta taattcacat aaacagagga gtagaaaatg aaaaaaaatc
    11281 gtgtatttgc tacagcaggt cttgttttat tagcagcagg tgtacttgca gcatgcagtt
    11341 cttcaaaatc atctgattca tcagccccta aagcttatgg ctatgtttat acagcagacc
    11401 cagaaacctt ggactacctg atttcaagta aaaatagtac aacagtagtg acttcaaatg
    11461 ggattgatgg tttattcact aacgataatt acggtaatct tgctcctgca gttgcagagg
    11521 attgggaagt ctctaaggat ggtttgacct acacttataa gattcgtaaa ggggttaaat
    11581 ggtttacctc tgatggagaa gaatatgcag aggtgacggc taaagatttc gtgaacggtt
    11641 taaaacacgc agcagataaa aaatcagaag ctatgtattt agctgaaaat tcggttaaag
    11701 gcttggcaga ttatctatca ggaacttcaa cagatttttc aacagttggt gtcaaggcgg
    11761 ttgatgatta tacgttacaa tacactttga accagcctga accgttctgg aactctaagt
    11821 tgacctattc tattttctgg cctctgaatg aagaattcga aacatcaaaa ggaagcgatt
    11881 ttgctaaacc aacagatccg acatccttgc tttataatgg tccattcttg ttgaaagggt
    11941 tgactgcaaa atcttctgta gagtttgtaa aaaatgagca atattgggat aaagaaaatg
    12001 tccacctaga tactatcaat ctagcttact atgatggatc agatcaggag tcgctagagc
    12061 gtaacttcac tagtggagct tatagttatg cccgtcttta ccctaccagc tccaactatt
    12121 ctaaggttgc agaagaatac aaggacaata tctattacac acaatcaggc tctgggattg
    12181 ctggtctggg tgtgaatatt gatcgccaaa gttacaacta tacttctaaa actacagatt
    12241 cagagaaagt agctactaag aaggcattgc ttaacaaaga tttccgtcaa gccttgaatt
    12301 ttgctcttga tcgctcagct tactcagctc aaatcaatgg taaagatgga gcagctttag
    12361 cagttcgtaa tttatttgta aaaccagact ttgtttcagc tggtgagaag acctttggtg
    12421 atttagtcgc tgctcaactt cctgcttatg gtgatgagtg gaaaggtgtg aatttagctg
    12481 atgggcagga tggtttattc aatgctgaca aggccaaggc agagtttgcg aaagctaaga
    12541 aagctttaga agcagacggc gttcagtttc ctattcatct ggacgttcca gtagaccaag
    12601 catcaaaaaa ctacatatct cgtattcagt cctttaaaca atctgtagaa acagttcttg
    12661 gtgttgaaaa tgtcgttgtt gatattcaac aaatgacaag tgatgaattc cttaatatta
    12721 cttactatgc tgccaatgct tcatctgagg attgggatgt atcaggagga gtttcatggg
    12781 gtccagacta tcaagaccca tctacttacc tggatatttt aaaaacaact agcagtgaaa
    12841 ctacaaaaac atatttagga tttgataatc caaatagccc ttcagtagtt caagttggtt
    12901 tgaaagaata cgataaatta gttgatgaag ctgccagaga gacaagcgac ttgaatgtcc
    12961 gttatgaaaa atatgcagcg gctcaagcat ggttgacaga tagttcactc tttattcctg
    13021 ctatggcttc ttctggtgca gcaccagtgc tttcacgaat tgttccattt actggagctt
    13081 ctgcgcaaac aggctctaag gggtcagatg tttacttcaa atatttgaaa ttacaagata
    13141 aagtggtgac taaggaagag tatgaaaaag ctcgtgaaaa atggttgaaa gaaaaagctg
    13201 aatcaaatga gaaagctcaa aaagaattgg caagtcatgt gaagtaaata atttctaata
    13261 gaactttctc tccatgagga gaaggttctt ttgggatttt taaaggaaat aatatgaaaa
    13321 aatatatttt tatgcgtgtt ttgcggtcat tggtttcgat tttcttagta acgactttga
    13381 cctacacgat tatctatacc ttggttcctc gaaaattgat tttcaagcag gatcctaact
    13441 ataataaaat tgcgacaacg gctgataaac gtgataacta tgaaaatact gtgtttgagc
    13501 gtatgggcta cattgagtat tacgatacta aagagttgca agaaaaggca agtagcatgg
    13561 attcttctgt aacagtagaa gcaaatgcga ccaataaagc tatttatgaa aagtacatca
    13621 atcaattagg tcatggttgg actttgggag aatttactga aagtggtcaa ttctatgcta
    13681 ctcgtgaaat tccaattttt gaacgtgttt ttcacttcta tgctaacttg attgacattg
    13741 accatacaaa taaaatccaa gaccctgaaa atccagactt gaaacgctac cttcgttttg
    13801 aaaatgatcc agctatcgga tggtcattgg tcggttcagg aactaaacat aaatatctct
    13861 tgtactttaa cagtcagttc ccatttgttc atcaaaactt tgtgaacttg aatttaggtg
    13921 actcttaccc aacctatgct aatacaccag ttcttcaggt tattactcaa ggtcaaggac
    13981 aaaccaaaac tgcccaagtt cagttcccaa caggtaagaa aacgtcttct gtaaatattt
    14041 actcaagaac ctacaagtca cctagtcagg ctgactctcg tgaagtagct agctatggga
    14101 aagatgatcc ttatacagcg actgaaagta attaccaata tccatctatg attgtcagct
    14161 ctgctattac tggtttgatt ggtttggttc ttgcctatgc tcttgccgtg ccacttggtt
    14221 cagccatggc tcgtttcaag aacacttgga ttgatagcct atcaacaggg actttgacct
    14281 tcttgcttgc tcttccaacg attgccttgg tttacatcgt tcgattgatt ggatcatcta
    14341 ttgcccttcc agattcattc cctatcttgg gagctggaga ttggcgttct tacgttttac
    14401 cagcagtcat ccttggtttg ttgggtgctc ctgttacagc catttggatt cgtcgttaca
    14461 tgattgactt gcaatctcaa gactttgttc gtttcgctcg tgcaaaaggt ttgtctgaaa
    14521 aagaaatttc aaacaaacac atctttaaaa atgccatggt tccgctggtt tcaggaattc
    14581 ctggtgccat tattggggtt atcggtggtg caacccttac tgaaacagtc ttcgccttcc
    14641 caggtatggg taaaatgttg attgactctg taaaagcatc taataactct atggtcgttg
    14701 gtcttgtctt catctttaca tgtatttcta tcttctcacg tcttttggga gatatttgga
    14761 tgactattat tgacccacgt attaaattga ctgagaaagg aggcaaataa tgtctacaat
    14821 cgataaagaa aaatttcagt ttgtaaaacg tgacgatttt gcctctgaaa ctattgatgc
    14881 gccagcatat tcttactgga aatcagtgtt taaacaattt atgaagaaaa aatcaactgt
    14941 agtcatgttg ggaatcttgg tagccatcat tttgataagt ttcatctacc caatgttttc
    15001 taagtttgat ttcaatgatg tcagcaaggt aaacgacttt agtgttcgtt atatcaagcc
    15061 aaatgcggag cattggttcg gtactgacag taacggtaaa tcgctctttg acggtgtctg
    15121 gttcggagct cgtaactcca tcctcatttc tgtgattgcg acagtgatta acttggttat
    15181 cggtgttttt gtcggtggta tttggggtat ttcaaaatca gttgaccgtg tcatgatgga
    15241 agtttacaac gtcatctcaa acatcccacc tcttttgatt gttattgtct tgacttactc
    15301 aatcggagct ggattctgga atctgatttt tgccatgagc gtaacaacat ggattggtat
    15361 tgccttcatg atccgtgtgc aaatcttgcg ctatcgtgac ttggaataca acttggcgtc
    15421 acgtactttg ggaacaccaa ccttgaagat tgttgccaaa aatatcatgc ctcaattggt
    15481 atctgttatt gtgacaacca tgactcaaat gcttccaagc tttatctcat acgaagcctt
    15541 cttgtctttc ttcggtcttg gattaccgat tacagtgcca agtttgggtc gtttgatttc
    15601 ggattattca caaaacgtaa caaccaatgc ttacttgttc tggattccat tgacaaccct
    15661 tgtcttggta tccttgtccc ttttcgtagt tggtcaaaac ttagcggatg ctagtgatcc
    15721 acgtacacat agataggagt agaaatgaca aaagaaaaaa atgtaatttt gactgctcgc
    15781 gatattgtcg tggaatttga cgttcgtgac aaagtattga cagccattcg cggcgtttcc
    15841 cttgaactag tcgaaggaga agtattagcc ttggtaggtg agtcaggatc aggtaaatct
    15901 gttttgacaa agaccttcac aggtatgctc gaagaaaatg gtcgcattgc ccaaggtagt
    15961 attgactacc gtggtcagga cttgacagct ttatcttctc acaaggattg ggaacaaatt
    16021 cgtggtgcta agattgcgac tatcttccag gacccaatga ctagtttgga ccccattaaa
    16081 acaattggta gtcagattac agaagttatt gtaaaacacc aaggaaaaac agctaaagaa
    16141 gcgaaagaat tggccattga ctacatgaat aaggttggca ttccagacgc agatagacgt
    16201 tttaatgaat acccattcca atattctgga ggaatgcgtc aacgtatcgt tattgctatt
    16261 gcccttgcct gccgacctga tgtcttgatc tgtgatgagc caacaactgc cttggatgta
    16321 actattcaag ctcagattat tgatttgcta aaatctttac aaaacgagta tcatttcaca
    16381 acaatcttta ttacccacga ccttggtgtg gtggcaagta ttgcggataa ggtagcggtt
    16441 atgtatgcag gggaaatcgt tgagtatgga acggttgagg aagtcttcta tgccccttgc
    16501 catccatata catggagtct cttgtctagc ttgcctcagc ttgctgatga taaaggggat
    16561 ctttactcaa tcccaggaac acctccgtca ctttatactg acctgaaagg ggatgctttt
    16621 gccttgcgtt ctgactacgc aatgcagatt gacttcgaac aaaaagctcc tcaattctca
    16681 gtatcagaga cacattgggc taaaacttgg cttcttcatg aggatgctcc aaaagtagaa
    16741 aaaccagctg tgattgcaaa tctccatgat aagatccgtg aaaaaatggg atttgcccat
    16801 ctggctgact aggaggaagg aaatgtctga aaaattagta gaaatcaaag atttagaaat
    16861 ttccttcggt gaaggaagta agaagtttgt cgcggttaaa aatgctaact tctttatcaa
    16921 caagggagaa actttctcgc ttgtaggtga gtccggtagt gggaaaacaa ctattggtcg
    16981 tgctatcatc ggtctaaatg atacaagtaa tggagatatc atttttgatg gtcaaaagat
    17041 taatggtaag aaatcgcgtg aacaagctgc ggaattgatt cgtcgaatcc agatgatttt
    17101 ccaagaccct gccgcaagtt tgaatgaacg tgcgactgtt gattatatta tttctgaagg
    17161 tctttacaat caccgtttat ttaaggatga agaagaacgt aaagagaaag ttcaaagtat
    17221 tatccgtgaa gtaggtcttc ttgctgagca cttgactcgt taccctcatg aattctcagg
    17281 cggtcaacgt caacgtatcg gtattgcccg tgccttggtc atgcaaccag actttgttat
    17341 tgcagatgag ccaatttcag ccttggacgt ttctgtacgt gcccaagtct tgaacttgct
    17401 caaaaaattc caaaaagagc tcggcttgac ctatctcttc atcgcccatg acttgtcggt
    17461 tgttcgcttt atttcagatc gtatcgcagt tatttacaag ggtgttattg tagaggttgc
    17521 agaaacagaa gaattgttta acaatccaat tcacccatat actcaagcct tgctttcagc
    17581 ggtaccaatc ccagatccaa tcttggaacg taagaaggtc ttgaaggttt acgacccaag
    17641 tcaacacgac tatgagactg ataagccatc tatggtagaa atccgtccag gtcactatgt
    17701 ttgggcgaac caagccgaat tagcacgtta tcaaaaagga ctaaactaat aatggtttta
    17761 taatttccat gtcaattttt atggaaatta taaaactttt tttgttggac tgatttgaaa
    17821 aactttgaaa gcttcctgaa agaattttcg aaaaatttta aaaattctga aatattattg
    17881 acaggtgatg aaaaaggtgg taggatagga agcatcaaaa aagaaagcag aaaaagaaat
    17941 gaataagaga caagccgtaa ctatgaatca aaacttttac tttagctact ttagatagtg
    18001 tttgtagaca tgtcaccatg gatgcaaaca gttcaagcaa tttgtttgta tctatgtggc
    18061 taagattttt gattgtggtc catatagatg aatatctaaa tggactagct aagttgtaac
    18121 ttgttaggtt atttcaagta tccgtttaga tagtatctag gcggtttttt gttttatctt
    18181 ttctgagaag gagtttcatt atgaaagttg ttcgaaaatc actagccccc tcttggtagt
    18241 ggggctcctc ttgacttctc tgatcagttt gcatcagttg aaggcagata agaaaaagat
    18301 gtctttcgta tcggtatttc gcaatttatt acccaccagt ccttagacgc tactagagaa
    18361 gaatttgtgg atgagctggc caaacaaggc tatgttgagg gggaaaatat tgagattgat
    18421 ttgcaaaatg tacagggaga acaaagaaat ctaaaaacga tttcccagca actagcagaa
    18481 tccagcgatg tcgttctagc catcgcaagg ccttctgctc agagcttggc caatacgacg
    18541 caaacgacac cagttatctt ttcagctgta acagacccag tcagtgccaa gttggttgag
    18601 tcaagagaac atcctggggg caatgtaact gggacaagcg atcagtcatc agatgccatt
    18661 tcaacccaaa tcaatttgat aaagaaagtg ttgccaaagg ctaaaacgat tggaatcctc
    18721 tatactcaga gcgagcccaa ttcggttgtt taaaatgatg aagctaagcg acttctgaaa
    18781 gaaaaaggct ttaccgttgt tgaaaaaaca atcttggaca gtaataacgt caaggcggca
    18841 gcagagagtt tgatggcaga ggtggatatg gtttttgtcc caactgataa tatcatttcg
    18901 tcaaccatgg aaacagttaa gcaggtttct attaaacaca aggttccagt attcggtggt
    18961 tcaacagaaa tgattgctgt tggtgacttg tataactacg gaaccaatta tgaagaattg
    19021 ggaagacaga cagctcgcat gctgattcgt gttttaaaag gtgagaaacc agaaaatata
    19081 gcagttgagt tgcctgaaaa actggaattg cataccaatc aagaaatggc agatgcattg
    19141 ggaattgata ttagtaagtt agaaggcaag gaataaggag gattaagatg aattttgtgt
    19201 tatctagttt atcagaaggt ttgttgtggt cgattgtggc gattggggtc tacttgactt
    19261 tccgtatttt ggatattgcg gatataactg ctgagggagc ctttcctctg ggggcagcag
    19321 tcgttgtatc acagatacag gcaggggcaa atccttggct tgcgacttta cttgctttgc
    19381 tggcaggtat ggtagcaggt cttgtatcag gaatgctcca caccaagatg aaaatcccag
    19441 ctctcttgac agggattgtg accttgacag ggctctattc aatcaatatt aaaattatgg
    19501 gcagtgtacc caatctttcc ttgggagatt ctgcaaccgt ctttaaacag ttggcgagct
    19561 tgggtctgac aaatgaagga gctgttttct cacttagttt agtctgtctc ttacttgttt
    19621 ggtcttgacc cttttgatga aaacagagat tggtttggtc ttgcgttcga ctggggataa
    19681 tatcccgata agtgaggcca atggagtcaa tgtagacacc atgaagattg ttggttacat
    19741 gatttcaaac ggtttgattg ccctatgtgg ttccttgttt gcccaaaatg atggattttc
    19801 agatgtgact tctgggacag gaaccattgt tgttggtttg agctcggaag tcttgataca
    19861 cgacttgacc attggaggcc gcttattatc catcggaatc ggtgctattg tttaccgttt
    19921 gattatttta aatatctatg aaattccaaa tctagatcaa aacttggtac gtctctttaa
    19981 tgcaatcttg ctcgctttgg ttttatttgc accagaattg caaaagagat taaagattcg
    20041 tggtctgaaa ttgagaaatg aataggagga gaaagttatg gcaagtttgt taacacttga
    20101 aaatattcac aagacatttg aagcagggac ggtcaatgag aaccatgtcc tcaaaggatt
    20161 agatttagag gttgaagagg gagattttat ctatgtgatt ggtggaaatg gagcaggaaa
    20221 atccactttg atgaatatct tggctggtaa tctatcggtg gacgaagggg accttctact
    20281 ggcagggaaa tccattaaaa atctgagtgt cagaaagaga gccaaggata ttgcccgtgt
    20341 ttttcaatat cctaagatgg gaacagcttc tcgtttgacg attgaggaga atatggctat
    20401 tgccttgaga cgtgggcaaa agagagggct tggttggggt gtgaaggaga aggatagaat
    20461 ccagttccaa gaggccttga aggagttgaa tattggtctt gaaaaccgct taaaagtaga
    20521 tactcaatat ctctcaggag gacaaagaca ggctttgacc ctagtcatgg cagctctggt
    20581 gaaacccaaa cttttacttt tggatgaaca tactgcggca cttgacccga aaaccagtca
    20641 aatggtgatg gacttgacgc aaaagattgt ggagcaccat cagtggacga ctttgatgat
    20701 tacccatgat atgaatcatg cgattgagta cggcaatcgt ctcattatgc tctatcaagg
    20761 caagatagtg gtggatgtca agggagaaga gaaaaagcat ctgacagttg aagaccttat
    20821 gcatctcttc cagaaaaata gtggccaaag tctggtcagt gatgaattag ttttgggata
    20881 aagaaaaagg ctgagatctg gtgtctcaat cttttatttc ttgcgttttg caaaatctac
    20941 aaatctaaat ttttctaact tatggcgact ttcggtaaac tgaaattggc gtccgtcttg
    21001 gagatagact tttgatttaa tcgaaacgat ataagggtct ttgccaatgt ccatgagaat
    21061 cttgtctcgg tcgcttgagt ggtcgatggt gatttccttc tgagcataat caataaggag
    21121 tttgaggcca ttttctatat aagaatagat agactgctca gcaatttggc gagtgagatt
    21181 tggaattagt tccatatcca gatagtccgt atccagaacg gataccagat catccaccac
    21241 acgctggcgg accaccttcc aaaccatccg aaactctggg aaaccggtta tcagtgagga
    21301 ttttttatca ataataatct tgtccagact gaccacgttg gttttagagc gcagtccaag
    21361 ttctttaact agttcttggt agctggttag gttggataca gggaaattga cggtttcttc
    21421 tttgacgact tgagaacctt gccctcttat ctttttgatc aatccttcct cttggaggag
    21481 agacagggcc tttcggacgg tatcacgact cacttgatat tgctccataa ggtcgtgctc
    21541 gctaggaagg aaatctccga cagcgtaagt ctcgttttga atggtttctt ggatttgctt
    21601 aaataattgt tggtatttct tcattttgcc tgctttaata ggtttttagt ctagtttaat
    21661 gaacacctgt atctatttta ccatacaagt gctagaaaat ttacaaaaaa acaccctttt
    21721 tataccaaac aagttgcaga caagtttggt atcgtttaca atatacttat caaatcaaac
    21781 ttgctttgac aagtataagg agaatcaaat gggaaaattt gaacaagaag ccaaagatct
    21841 gcttcaggca attggaggca aagaaaatgt gactgccgta actcactgtg cgacacggat
    21901 gcgctttgtt ttaagagatg ataagaaggc taatgttaaa gctatcgagt caattccagc
    21961 tgttaaagga acttttacaa atgcaggtca atttcaggta atcattggaa atgacgtgcc
    22021 catcttttat aatgatttta cagccgtttc aggtattgag ggtgtttcca aagaagcaac
    22081 caagtctgca gctaagagta atcaaaacgt ggttcaaggt gttatgacca ctctggcgga
    22141 aatctttact ccgattattc cagccttgat tgttggtgga ttgatcctcg gtttccgtaa
    22201 tgtcttggaa ggtgtccatt ggtcgatgtt ggatggcaag accatcacag aatcctctca
    22261 gttttgggca ggggttaatc acttcctctg gttgcctggt gaagctatct tccagttctt
    22321 accagtaggg attacttggt ctgtttctcg taagatggga accagccaaa ttttgggaat
    22381 tgttctcgga atctgtttgg tatcgcctca gttgctcaat gcctatgcgg ttgcttcaac
    22441 gccagcagct gatatcgcgg caaactgggt ttggaatttt ggttatttta ctgttaatcg
    22501 tattggttac caagtccaag ttatcccagc cttgcttgca ggtttgagtc tgtcttatct
    22561 tgaaattttc tggcgcaaac atatcccaga agtcatttcc atgatttttg tacctttttt
    22621 gtcattgatt ccagccttga ttttggctca tactgttttg ggaccaatcg gttggacaat
    22681 tggacaagga ctttcatcag ttgtcttggc aggtttaact ggtccagtta aatggctctt
    22741 cggtgcaatt tttggtgccc tctacgctcc atttgtcatc acaggtctgc accatatgac
    22801 caatgccatt gatacacaat tgattgcgga tgctggtggc actgccctat ggccaatgat
    22861 tgctctttct aatattgctc aaggctcagc cgtgtttgcc tattatttca tgcatcgcca
    22921 tgatgagcgt gaggctcagg tttcacttcc tgcaaccatt tcagcctatc tcggtgttac
    22981 agaaccagct ctttttgggg ttaacgtaaa atatatttat ccatttgttg ctgggatgac
    23041 tggttcagcc cttgcaggca tgttatccgt tacttttaat gtaactgcgg cttctattgg
    23101 tatcggtggt ttgccaggta ttctctctat tcaacctcaa tacatgctgc catttgcagg
    23161 aactatgcta gttgcgattg ttgttccaat gctcttgact ttcttcttcc gcaaggctgg
    23221 tctctttaca aaaacagagg gcgatacgaa cttgcaggca gaattcgttg ctcaagaaga
    23281 agcagaattt gtgaaccatg aaccagtaga acttacttcg gtagaaatta tcagcccact
    23341 aactggccaa gtgaaagaat tgagtcaagc gacggatcct atttttgcat caggtgtcat
    23401 ggggcaaggt ctagtcattg aaccaagcca aggtgagttg acctctccag ttaatgggac
    23461 agtgacggtt cttttcccta ccaagcatgc catcggcatt gtctctgacg agggagttga
    23521 attgctcatc cacatcggta tggatacagt aggtcttgat ggcaaaggtt ttgaaagtct
    23581 tgtagtccaa ggagatcacg ttacagttgg tcagcaactg attcgttttg atatggatgt
    23641 cattaaggct gcaggtctgg tgacagaaac tcctgttatc atcaccaacc aagatgctta
    23701 tacagcgact attcccggaa cttatccgac aacgatccaa gctggagcat ctctcatggt
    23761 cgctacacga atctaatact cttcgaaaat ctcttcaaac cacgtcagct ctatctgcaa
    23821 cctcaaaata gtgttttgag caacctgtgg ctagcttcct agtttgttct ttgattttca
    23881 ttgagtataa ttgaaatagc acaaataggg acggaagact gcttcctccc ttttatcaga
    23941 aaaggagaaa cagatgacac ttgataaagg aaaagtagtc tatcaaatct atccaaaatc
    24001 ttacaaagac accactgaaa acggtattgg ggatttccgt gggattatcg aaaaaattcc
    24061 ctatttagcc aaactaggtg tggatatggt ctggctcaat ccattctatc caagccctca
    24121 acgggataat ggttacgata tttcagatta tatggcagtg gatcctcttt ttggtgatat
    24181 ggctgatttt gaggaaatgg tgtgtgtcgg taaagagcac aagattgact ttatgctgga
    24241 catggtactt aatcattgtt cgacagagca tgaatggttt cagaaagccc tagctggtga
    24301 taagtattat caagactttt tcttcatcca agaccaacca acagattggc agtctaagtt
    24361 tggtggttct gcatggacgc ctttcgggga tactgggaaa tactatcttc acctatttga
    24421 tgagacccag gctgacctta actggcgcaa ttccaatgtc cgtaaggagc ttttcaaggt
    24481 tgttaatttc tggcgcgaca agggtgtcaa aggtttccga tttgatgtga tcaatttgat
    24541 cggtaaggac gaggtttcag tggactgtcc tgaaaatgaa gggaagccag cttatacaga
    24601 caaacccatc gttcataact atttgcgtat gatgaaccaa gccacttttg gatctgacga
    24661 tagctttatg acagttgggg aaatgtcttc taccaccatg gaaaactgtg tcctctattc
    24721 gtcgcctgat cgtcaggaat tatccatgac ctttaatttt catcacctca aggtggacta
    24781 taaggatgga caaaagtgga ccttggctcc ctttgatttt gaagagttga agagtcttta
    24841 ccatagttgg ggtaaggaaa tgagtgataa agacggttgg agtgccctct tttggaacaa
    24901 tcacgatcaa ccacgtgcct tgaaccgttt tgtcgatatt caaaacttcc gcaaggaagg
    24961 agccaccatg ctagcagcca gcattcacct gtcacgtggg acaccttata tctacatggg
    25021 tgaggaaatc gggatgattg acccagacta tgattctatg gctgattatg tggatgtcga
    25081 atcgcttaat gcctatcaga tgctcttaga agaaggaaag agtcagcaag aagccttcca
    25141 gattattcaa gctaagtcgc gtgataattc acgcattccc atgcagtggg atgcttcgga
    25201 aaatgcagga ttttcaacag gcactccttg gttgaaagct ggtaaatcct ataaatacat
    25261 caatgtagaa aatgaaatcc agggtccgat ttttaccttc tatcaagact tgattcgact
    25321 gcgtaaggaa atgccaatca tttcagaagg aagttacaaa ccagcctttg aagatagcaa
    25381 acaagtctac gcttttgaac gccagtttga ggatcaaaag ttactagtgc tcaataactt
    25441 ttatgctaaa gaagtggaaa tcgacttgcc agcagtctac caaaatggac agattttgat
    25501 atcaaactat gaagatgcag aagtgtctga gaaaattcta ctcaaaccct atcaaacact
    25561 ggctatttat gtaaattaat actcttcgaa aatctcttca aaccgtgtca gttttatctg
    25621 caacctcaaa gcggtgcttt gagcaacctg tggctagctt cctagtttgc tctttgattt
    25681 tcattgagta taaacttagc aaggagacct ctttttcaga aaagcagggt tctttttgct
    25741 gtctattcca tccctgaaag tgtagaacaa actggtcagg gtggctagtt tgtggtataa
    25801 tgaaaggaac tcaaaaagaa acaggagaaa aatgatggat ttacttttag caattgtatt
    25861 gattgtgcta gcttttctag gaggagctct tggaggaatg tacttggttc gtaagcaaat
    25921 cgaaaaagaa tttgctgaca acccacgttt gaatgctgaa gcagttcgta ctcttttgag
    25981 tgcaaatggt caaaaaccaa gcgaagctaa ggtacaacaa gtttaccacc aaatcatccg
    26041 ccaacaaaag gcagcccttg ctaacaataa aaagaaaaaa taaataagga aaagtctggg
    26101 atgaaagttc cagacttctc actatgttgg ttatggttta gggatgagac tttttccttg
    26161 cattctattg atatttgatt atgattaaga gagaggtagt tgacattcta tcaagctgtc
    26221 ggtcagttcg ttcagtacaa ggaatcataa tgaacgatca atcagaaaaa agactagaaa
    26281 gaagactgta tggataatcg accaattggt tttttggatt cgggtgtcgg gggcttgacc
    26341 gttgtgcgcg agctcatgcg ccagcttccc catgaagaaa tcgtctatat tggagattcg
    26401 gcgcgggcgc cctatggccc ccgtcctgct gagcaaattc gtgaatatac ttggcagctg
    26461 gtcaactttc tcttgactaa ggatgtcaaa atgattgtca ttgcttgtaa cactgcgact
    26521 gcggtcgtct gggaagaaat caaggctcaa ctagatattc ctgtcttggg tgtaattttg
    26581 ccaggagctt cggcagccat caagtccagt caaggtggga aaatcggagt gattggaacg
    26641 cccatgacgg tacaatcaga catataccgt cagaaaatcc atgatctgga tcccgactta
    26701 caggtggaga gcttggcctg tcccaagttt gctcccttgg ttgagtcagg tgccctgtca
    26761 accagtgtta ccaagaaggt ggtctatgaa accctgcgtc ccttggttgg aaaggtggat
    26821 agcctgattt tgggctgtac tcattatcca ctccttcgcc ctattatcca aaatgtgatg
    26881 gggccaaagg ttcagctcat cgatagtggg gcagagtgcg tacgggatat ctcagtctta
    26941 ctcaattatt ttgaaatcaa tcgtggtcgc gatgctggac cactccatca ccgtttttac
    27001 acaacagcca gtagccaaag ttttgcacaa attggtgaag aatggctgga aaaagagatt
    27061 catgtggagc atgtagaatt atgacaaata aaatttatga atataaggat gaccaggact
    27121 ggtatgttgg gtcttatagt atttttggtg gcgttaacag tttgagcgac tataagacag
    27181 attttcctct gtttgaattc tccaaaatat ttggagatga agagtatggt ttcccgcttt
    27241 cagttactgt tttacgctat ggttctacct accgtttgtt ctcctttgtg gtagacatgc
    27301 ttaatcaaga aatgggacga aacttggaag ttattcaacg tcatggggcc ctgctcttgg
    27361 ttgaaaatgg gcaactcttg tatgtagaat tgcctaaaga aggggtcaat gttcatgatt
    27421 tctttgagac aagcaaggtc agagaaacct tgttgattgc gactcgtaac gaaggtaaaa
    27481 ccaaggaatt ccgagctatc tttgataagt taggctacga tgtggaaaat cttaatgcct
    27541 accctgacct gcctgaagta gcagaaacag gtatgacctt tgaagaaaat gcccgcctta
    27601 aggcagaaac catttctcaa ttaacgggca agatggtttt ggcagatgat tctggtctca
    27661 aagtcgatgt ccttggtggc ttaccaggcg tctggtcagc tcgtttcgca ggtgtgggag
    27721 caactgaccg tgaaaataat gccaaactct tgcacgaatt ggccatggtc tttgaactca
    27781 aggaccgctc ggctcagttc cacacaaccc tagtcgtagc cagcccaaat aaggaaagtt
    27841 tagttgttga agcagactgg tcaggttata ttaactttga acctaagggt gaaaatggct
    27901 ttggctatga tcccctcttc cttgtaggag aaacaggtga gtcatcagct gaattaaccc
    27961 tggaagaaaa aaatagtcaa tctcaccgtg ccttagccgt taagaaactt ttggaggtat
    28021 ttccatcatg gcaaagcaaa ccatcattgt aatgagcgat tcccatggcg atagcttgat
    28081 tgtggaagaa gtccgtgatc gctatgtggg caaagtcgat gccgtttttc ataacggcga
    28141 ttctgaacta cgtccggatt ctccactttg ggagggcatc cgcgttgtta aagggaacat
    28201 ggacttctac gccggctacc cagaacgtct ggtgactgag cttggttcga ccaagattat
    28261 ccaaactcat ggtcacttgt ttgacatcaa tttcaacttt caaaagttgg actactgggc
    28321 tcaggaggaa gaggccgcta tctgcctcta tggtcacttg catgtgccaa gtgcttggat
    28381 ggaaggcaag atcctctttc taaatccagg ttctatcagc caaccacgag gtaccatcag
    28441 agaatgtctt tatgctcgtg tggagattga tgatagttac ttcaaagtgg actttttgac
    28501 acgagatcac gaggtgtatc caggtttgtc caaggagttt agtcgatgat tgccaaggag
    28561 tttgagactt tcttgttggg gcaggaggaa acttttttga cccctgctaa aaatctagct
    28621 gtgttgattg atacccacaa tgcggatcat gcgaccctct tgctcagtca gatgacctat
    28681 acccgtgttc ccgttgtgac agatgaaaaa cagtttgttg ggacgattgg actcagagat
    28741 attatggctt atcagatgga gcatgacttg agccaagaaa tcatggcgga tacggatatc
    28801 gttcatatga caaaaacgga cgtagcggtt gtttcgcctg atttcaccat tacggaggtc
    28861 ttgcacaagc tagtagatga gtccttctta ccggttgtgg atgcagaggg tattttccaa
    28921 gggattatta cgcgcaagtc catcctcaag gccgttaatg ccctcttgca tgactttagt
    28981 aaggaatatg agattcgatg ccaatgagag acaggatttc agccttttta gaggaaaagc
    29041 agggcttgtc tgtcaattcc aagcagtcct ataagtatga tttggagcaa tttttagaca
    29101 tggtaggtga gcggatttct gagaccagtc tcaagattta ccaagcccag ctagccaatc
    29161 taaaaatcag cgcccagaag cgaaagattt cggcctgtaa ccaatttctc tactttctct
    29221 atcaaaaagg agaggtggac agcttttacc gcttggaatt agccaaacaa gctgaaaaga
    29281 agacggaaaa gccagagatt ctatacctag actctttttg gcaggaaagc gaccatccag
    29341 agggccgctt gctagcgctc ttaatcctag aaatggggct cttgcccagt gagattttag
    29401 ccatcaaggt tgcggacatc aatctggatt ttcaggtgct gcgaatcagc aaggcttccc
    29461 aacagaggat tgtcaccatt cccacggcct tgctttcaga attggaaccc ttgatggggc
    29521 agacctatct ttttgaaaga ggagagaaac cctattctcg tcagtgggcc tttcgtcagt
    29581 tagaatcttt tgtcaaggag aaaggttttc catccttatc agctcaagtc ttacgtgaac
    29641 agtttattct aagacaaata gaaaacaagg tcgatttgta cgaaattgca aaaaaattag
    29701 gattaaaaac agtcctgacc ttagaaaaat atagataatg gatattaaat taaaagattt
    29761 tgaaggaccc ctggacttac tcttgcatct ggtttctaag taccagatgg atatctacga
    29821 tgtgcccatt acggaagtca tcgaacagta tctagcctat gtctcaaccc tgcaggccat
    29881 gcgtctggaa gtgacgggtg agtacatggt catggctagt cagctcatgc tgattaagag
    29941 tcgtaaactc cttccgaagg tagcagaagt gacagacttg ggggatgacc tggagcagga
    30001 cctcctctct caaatcgaag aatatcgcaa gttcaagctc ttgggtgagc acttggaagc
    30061 caagcaccaa gaacgggccc agtattattc caaagcgccg acagagttga tttacgaaga
    30121 tgcggagctt gtgcatgaca agacgaccat tgacctcttt ttggcttttt caaatatcct
    30181 agccaagaaa aaagaggagt ttgcacaaaa tcacacgact atcttgcggg atgagtataa
    30241 gattgaggac atgatgatta tcgtgaaaga gtccttgatt ggacgagatc aattgcgctt
    30301 gcaggatttg ttcaaggaag cccagaatgt ccaagaggtc atcaccctct ttttggcaac
    30361 cctagagtta atcaaaaccc aggagctgat cctcgtgcaa gaggagagtt tcggagatat
    30421 ctatctcatg gaaaagaagg aagaaagtca agtgcctcaa agctagactt gatagagagg
    30481 aaagatgagt actttagcaa aaatagaagc gctcttgttt gtagcgggtg aagatgggat
    30541 tcgggtccgc cagttagctg aactcctctc tctgccaccg acaggcatcc agcaaagttt
    30601 aggaaaatta gcccagaagt atgaaaagga cccagattcc agtttggctt tgattgagac
    30661 aagtggtgct tatagattgg tgaccaagcc tcaatttgca gagattttga aggaatactc
    30721 taaggcgcct atcaaccaga gcttgtctcg ggctgccctt gagaccttgt ccattattgc
    30781 ctacaaacag ccgattacgc ggatagaaat tgatgccatc cgtggagtta actcgagtgg
    30841 agccttggca aagttgcagg cttttgacct gataaaggaa gacgggaaaa aggaagtatt
    30901 ggggcgcccc aacctctatg tgactacgga ttatttccta gattacatgg ggataaacca
    30961 tttagaagaa ttaccagtga ttgatgagct tgagattcaa gcccaagaaa gccaattatt
    31021 tggtgaaagg atagaagaag atgagaatca ataagtatat tgcccacgca ggtgtggcca
    31081 gtaggagaaa agcagaagag ctgattaagc aaggcttggt gacggttaac ggccaagtgg
    31141 tgcgtgaact agcaaccact atcaagtcag gcgacaaggt cgaagttgaa ggtcaaccta
    31201 tctacaacga agaaaaggtc tactatctgc ttaacaaacc acgcggtgtg atttccagtg
    31261 tgacagatga taagggtcgc aagacggttg tcgacctctt gcccaatgtc aaagagcgta
    31321 tttaccctgt gggtcgtttg gactgggata catcaggtgt cttgattttg accaatgatg
    31381 gggactttac agacgagatg attcaccctc gtaatgagat tgacaaggtt tatgtcgcgc
    31441 gtgttaaagg tgtggccaat aaggacaatc tccgcccctt gacccgtggt cttgagattg
    31501 atggtaagaa aaccaagcca gctgtttatg aaattctcaa agtggaccca gtcaaaaatc
    31561 gctctgtggt gcagttgacc atccatgaag ggcgtaacca tcaggttaaa aagatgtttg
    31621 aagctgttgg tctccaagta gataagttgt ctcggactcg tttcggacac ctagacttga
    31681 caggactccg tccaggagaa tcccgtcgtc ttaataaaaa agaaatcagc caactacaca
    31741 ccatggctgt aactaaaaaa taatgaaacg aattttaata gcgcctgtgc gcttttacca
    31801 acgttttatc tcaccagtct ttccaccctc ttgtcgcttt gagctgactt gttccaacta
    31861 catgattcag gctattgaaa aacatggttt taagggggta ttgatgggct tggctcggat
    31921 tttacgttgt catccctggt cgaaaacagg taaggatccc gttccagacc acttttccct
    31981 taaacgaaat caagaagggg aatgaggtgg ggtaaataga tttcaaaatg ataaaaacgc
    32041 atcctatcag gtttgagtga acttgatagg atgcgtttta gaatgtcaaa attttatact
    32101 cttcgaaaat ctcttcaaac cgcatcagct ttcatctgca acctcaaaac agtgttttga
    32161 gcaacctgcg gctagtttcc tagtttgctc tttgattttc attgagtatt aaattgagtt
    32221 tgaagtggct tatttcaaag ctttttgtat gtcttcaatc atgagttttg ttgattcaag
    32281 tccgcctccg cttagatacc agaggtctgg tgttagttgg ataatcttac catttttagc
    32341 agcaggtgtt tcagcgataa gggcattttc taggacaccg tcgttgctag agttgtcccc
    32401 accgatggca agggtacggt tgatgacaaa gaggatgtca gggttgattt ctttgacact
    32461 ttcaaagctg acttcttgtc cgtggcgtga gtcttcaaat tttgtatcag ttggtttgaa
    32521 tttcaaggtt tggtataaga aagagaaacg agatttggca ccaaaggctg ccatttttcc
    32581 ttcattaagg aggatcgcaa gggctttttt gtcagagctt tcatttttag tagcgacttc
    32641 ttggatgctc ttgtctagct tggccaattc ttccttggct ttttgtatac tagtttcgcc
    32701 gaaggcactt gctaaggatt cgatattagc cttgatagaa gtccagtagt cgtccttgct
    32761 tgcttggaag agaacggttg gggcgatttc tttgaatttg tctacgaatt tttgtgtacg
    32821 tggtgaagcg ataatcaaat caggctcaag ggcggcgata gcttctaaat caggttcttt
    32881 catagaacca acatttttga cagttcccac taggtctttt agataagtcg gaatagtttt
    32941 tgtaggcatt ccgatgatat ttttttcaaa tcctaaagcg cgaatagtat ccgcagcgcc
    33001 gaggtcaaag gtcacaatct tttcaggaac tttggaaagt ttgacctcgt ccagtgaact
    33061 tttaatggtt acctctgttg gagcagagct actggtctct gtctgactag tgcttgagtt
    33121 tgtactacat gcaccaagta ggagcaagaa gctggccact agggcagtga aataaagttt
    33181 aagggatgtt ttcataattt ctccttttta aaatgtgata acgatttagg gagtctctta
    33241 atcttattga ctaagagact gaaggttctc taacttgagc ttttatgtta ctagctatag
    33301 atacagatct ttttgtcatt gatatcagct agcgtgatgg gaatctcata aagttgactg
    33361 agcaggtcag cctgcatgat ttgatcggtt cttcccttgc taaagacctg gccgtccttg
    33421 aaggcgacaa tttcatctgc atactgactg gccatgttga tatcgtggag gacgatgata
    33481 atggtcttgc cgagttcctc caccagtcgt cgaagaatct gcatcatgct gacgctttgc
    33541 ttgatatcga gattgttgag tggttcgtcc agcaagataa agtccgtatc ctgggccagt
    33601 accatagcga taaagacgcg ctggagttgc ccccctgaca ggctattgat gtagcggtct
    33661 tttaagttgg tcagttccaa atagttcaga gtttctcgga ttttttccca gtcttctgat
    33721 ctaagtcgac ctcggctgta gggaaaacgt ccaaaactga ccagttcttc aacagtcaat
    33781 ttggcttggt aattgatttt ctgttttagg atggttagtt cttgggccag ttcttgcgaa
    33841 ttccagctct cgatttcacg tcctttgata ctgagaactc cctgatcttt cttggttagt
    33901 ctgctcatga tggagaggag agtcgatttt ccagcaccat ttggaccaat aaaggctgtc
    33961 agtttttgag gactgacttc aagcgaaatg ccttgcaaaa tatcctgttt ttgaatggat
    34021 ttgtcaatgt tttccagttt cactgacgag acctcctata tagtaagata aagaataaga
    34081 agccacccac actctcaatg atcatactga tacgaatttc cagtgcaaag actcgttcaa
    34141 tcaaggcttg ccccaaggtt aagctaataa atccaaccag aatggccact ataaagagta
    34201 acttgtgctg atagtctttg acaatcaggt aggtgaggtt agccagcata aagccgaaga
    34261 aggccatagg tcctaccaag gcagtggccg ttgaggtcaa aagcacgatt ccccagagga
    34321 gctctttctg ttctttttca acatcgagtc ccaatatctg agccgtttct ctttgcaggt
    34381 gcaagacatc tagaacgact gcttttcgaa agaaaaagat tgtcaaagcg aggatgatca
    34441 gagaaccgat ggctaggatg gaagtgttga gatgttgaaa ggaggcaaaa agactatttt
    34501 gcagtttatc gtattcgttt ggatccatta ggacttgaag gaaggtgcta atatttcgaa
    34561 agagacttct gagcgctaga cagatcagca ggatgaagac caggtcttgc ttcatcagtg
    34621 tcttcaagta accttgtaag gcgagaaaga agaaggactg gacaagaagt aagactagga
    34681 attctaagat aggggatttg ccaagttgaa gaaacttgct ttcaaaaacc agtagtaggg
    34741 tttgtagtag gacgtagaag gattcaattc ccaaaatact aggcgtcagg aagcgatttt
    34801 ccgtcagggt ttgaaaacta atggtcgaaa tcccagtcgc gatggctacc aagagataaa
    34861 cgatgatctt ttgggaacgc aacttccaag caaaggctga caagtgagtg atgggccaaa
    34921 agtagagaag acaagctccg atggcaagaa taatgagaat ccagaagagc ttggtatgtt
    34981 tgcttttagt ctgcatcttt tcgtccccct ctccagagaa gtaggataaa gacgagacta
    35041 ccgatgattc ctagcaagag actgacagac aactcatagg gcctaatcag aactcgggat
    35101 aggatatcgc aagccagaac tagattggca ccaaccagtg cgaccatgag tttggtttga
    35161 cttagattat ctccatagcg cttgcgaaca agattgggaa cgataactcc gagaaatggt
    35221 aagccaccca cggtaatcat ggtgacgctt gtcgttagcg ccaccagaaa gagggccagt
    35281 ttttcaagta gggagtagga aatccccaaa ctctcgctgg tttctttccc cagattcatg
    35341 atggtgaagg tttgggataa tttccaaacg gttatcagga tgatgaggcc taagaagagc
    35401 cactcatact gatgggtctg aatcatggag aaggagccct gggtccaggc agtcatactc
    35461 tgaaccagat tgaaacgata ggcgataact tctgtgactg agccgataat cccgctatag
    35521 atgatcccaa tcagaggcaa catccacctt tcctttacag taaaaatggt cataaaggct
    35581 aggaagaaga gggtgaatac gatggatgaa acaaaagcga agagcatctt ttgggtcaga
    35641 ctagccgatg gaaagacaaa aaggctcagc accattccca gtttggcggc ttcagtcgtt
    35701 ccaactgtac tcggtgcagc aaactgattt tgggtaatag tctgcatgag aaggcctgcc
    35761 atactcatac tagaggcagt caggagaata ctgatagttc ttgggagacg ggactcttga
    35821 aagaggagcc aggtctgctg gtcgaaatca aatagctttc cccatgaaaa atcactggtc
    35881 ccaatgctaa tagagagaaa gactaggagt agaagtaagc caattaaata atgagaaagt
    35941 ttcatacccc gtcctttcat gtagatttgg tatcgaaaga tatctgcgga tataaatgta
    36001 acattatttt tctaatctgt caataaaatt tctgacaatt taataaatac aacaaggaga
    36061 gagcaacaag actttctcct ttgttatcct attctaaaat gtttttacct taatctgata
    36121 aaataatatc ttcgagggag tagctagccg tccaatcaag atattgttta gcttttgaag
    36181 catctgctag gacactggct gggtcactag cacgtcgagc aacaatctcg tgtgggattt
    36241 tttaatttag taattcttca gcagttttaa agatttcttt gatagtatag ccttttttag
    36301 tttctaagtt aaagatttga gaagaactgt cttcttgaaa taggtagttc attcctttaa
    36361 catgagccta tgcaaggtcc aagacataaa tgtaatctcg aatacatgaa ccgtcacgtg
    36421 tatcgtagtc atctccaaat atttttaagc tatcattttg tcccaatgcg gtcttgttga
    36481 tatttggaat gatgtgagtt ggatttttca cacgcagacc gtttgaagca tccatttcag
    36541 ccccagcagc attaaagtaa cggaaaataa catatttcca gtcgtagcga ttggccatcc
    36601 agtaaatcat tcgttcgccc atcagttttg tctctgcata agggttgaca gggtcgagca
    36661 gggtatcttc agtcaccggc ttgtcaatac agttatttcc atagagagaa gcagtcgaag
    36721 agaacatgat tttttgaatg ccaacttcag ataagacttt gagaacttgg ttcataccag
    36781 caacgttggc agtgaagtat ttacttggat tttcaatact ttcgctcaca acagtttcac
    36841 ctgcacaatg gagggcagca tcaatctgat tttcttctaa gtaagacttt aaggcgctag
    36901 catcataaac atccagttct ttaaagctag cacgactatc cacagccgct cgatttcctg
    36961 tagagagatt atcgaggaca tgcacctgat agccagcatt taaaagagct ttaacggtat
    37021 gggagccaat gtagccagtc tcacctgtaa caagaattgt tttggtcatg attttttcct
    37081 tttctagttt tgtactatag tagattgaaa taagatatga ataactctat taggaaagtc
    37141 aaattaattt ctagaaatgt tttagcagct acagcgtact attccaaact caaccaacta
    37201 tatatttaaa gaaagaagaa agggaagtca actattttct gtaaatttca tgaaaaatct
    37261 aaacaacttt gatggtgtat tgttttttgt ttggaaataa atattcgagt taattttgga
    37321 atgtttacta gattttgcac tacatgcaag agaatactat ccttttttaa gatagagcag
    37381 gactaagata ctaacttaga aatccacctt cccactatcc ttctcctata aaaagtggta
    37441 aaatggatga aagaaagaag gaactgaaat gacaacatta ttttcaaaaa ttaaagaagt
    37501 aacagaactt gctgcagtct caggtcatga agcgcctgtc cgtgcttatc ttcgtgaaaa
    37561 gttgacaccg catgtggatg aagtggtgac agatggcttg ggtggtattt ttggtatcaa
    37621 acattcagaa gctgtggatg caccgcgcgt cttggtcgct tctcatatgg acgaagttgg
    37681 ttttatggtc agcgaaatca agccagatgg taccttccgt gtcgtagaaa tcggtggctg
    37741 gaaccccatg gtagttagca gccaacgttt caaactcttg actcgtgatg gtcatgaaat
    37801 tcctgtgatt tcaggttctg ttcctccgca tttgactcgt ggaaaggggg gaccaaccat
    37861 gccagccatt gccgatatcg tttttgatgg tggttttgcg gacaaggctg aggcagaaag
    37921 ttttggcatc cgtcctggtg ataccattgt accagatagt tctgcaattt tgacagccaa
    37981 tgaaaaaaat atcatctcaa aagcttggga taaccgctac ggtgtcctca tggtaagcga
    38041 gctagctgaa gctttatcag gtcaaaaact cggcaatgaa ctctatctgg gttctaacgt
    38101 ccaagaagaa gttggtctgc gtggcgctca tacctctaca accaagtttg acccagaagt
    38161 cttcctcgca gttgattgct caccagcagg tgatgtctac ggtggtcaag gcaagattgg
    38221 agatggaacc ttgattcgtt tctatgatcc aggtcacttg cttcttccag ggatgaagga
    38281 tttccttttg acaacggctg aagaagctgg tatcaagtac caatactact gtggtaaagg
    38341 cggaacagat gcaggtgcag ctcatctgaa aaatggtggt gtcccatcaa caactatcgg
    38401 tgtctgcgct cgttatatcc attctcacca aaccctctat gcaatggatg acttcctaga
    38461 agcgcaagct ttcttacaag ccttgatgaa gaaattggat cgttcaacgg ttgatttgat
    38521 taaacattat taaaccataa gggaggtggt agggatggta gaaccaaacc tagaaagcct
    38581 tataaaagat ctttacaatc atgctcgaca tgatttgagt gaagatttag ttgctgctct
    38641 cctagagact actaaaaaac tgcctactac aaatgagcaa ttgcaggcag ttcgtctctc
    38701 aggcctggtc aatcgtgaat tgctcctaaa tcccaaacat ccagcacctg agttgctcaa
    38761 cttggctagc tttatcaaaa gagaagaatc caagtacaga ggaactgcga cttctgcgct
    38821 tatgtatgag gaactcttta aaatgctttg attccattgt ggatcaaagc attttttata
    38881 agacctatct cgtgagcgtt caaatagtag taacaaggaa tcatgagaag tcagccgaga
    38941 ccatagtagt gaagaaactt ctgtaatgga agtggagcga agtgaggaat cgtcaaacgg
    39001 taggggatag gctgtttgaa gggtgctacg aaaaagatag ggtaaggtga aattataacg
    39061 ggatgaaata agatatgaac aaattgattg aggaaattaa atgaatttat agaaatcttt
    39121 tagcagtcat ggtgtcctat tccagttgca aaatgctata aatacgaaca aattgattgg
    39181 gaaattcaaa tcgcttttta tcaatgtttt agaagataca gtgccctagt tcaacttcaa
    39241 tatactataa aagaacagat ggaagcaatt atgatttact tctatcttta aaataaatgc
    39301 tctgatagga aaaatgtgtc ccctcataaa atataggata gtacggatgg aaggcttttg
    39361 tagactggag gacgaaggaa tttagaaact ctcgttaaaa gttagttgag ccggagtatg
    39421 gtggtgtgga taattatcaa aaattatttc caaatcgaaa tagttgacag atagaacgat
    39481 gagtgttaca attgttttat tcatttcgat atcgaaataa attggaggtg ttatgagaga
    39541 tagtcatttg ctagcccatc atattcgttt gttgaatggg cggatttttc aaaagttact
    39601 gagtcaagat cctgaggctc tttatagggg tgaacagggc aagattttag cggttttatg
    39661 gaatagtgaa actggctgcg caactgcgac agatatcgcg cttgcgactg ggcttgcgaa
    39721 taatacgctg acgactatga taaaaaagct agaggaacaa aagcttgtaa ttgttagtcc
    39781 gtgtggaaaa gacaagcgta agaagtattt agttttaacg gagttaggca agtcccagaa
    39841 agaagtgggg catcgtgtca gtcagaaatt ggatactatc ttttacaaag gattttcaga
    39901 ggaagaaatt caccaatttg aaggttttca agaaagaatt ttggcgaatc tgaaagagaa
    39961 gggaaatgag gtttagaata tgtcacaaca agtgaaaaat gctcacaacc tatacatcca
    40021 tgccattcaa gacggacgag ttgctgaggc tcaagcccag tctgtagggg atacctatat
    40081 ccaacattcg acaggtgttc ctgacgggaa agaaggattt gcagccttct ttgcagattt
    40141 ctttgagcgt catccagagc gtcagattaa gattgtccgc accattgagg atggcaatct
    40201 ggtctttgtt catgttcatc aatatctgaa tggtggagaa gctcaatggg tgacgacgga
    40261 tactttccgt gcggatgaga atggtcgtat tgttgagcat tgggatgtcg ttgactacta
    40321 tcgaacacct gaaaatgacc aactagatca aatttttgga gattttgaaa tcaaggattt
    40381 ggacaagaaa gcagaaaata aaaagctggt tcgccgtttc ttgacagaaa ttttccaaaa
    40441 tggggagcta gagcagtgga gtgattatgt ggcagacgat ttgattcagc ataatcatga
    40501 tattggacaa ggaagtgctg cttataaaaa ctatgtggct gaatatagtg tcacttttga
    40561 ctttgttttc caactcttag gacaaggaaa ctatgtggtt agctatggtc agactcagat
    40621 tgatggcgtt gcttatgccc agtacgatat cttccgttta gagaacggga aaattgtgga
    40681 gcattgggat aataaggaag ttatgcctaa ggtagaagac ttgaccaatc gagggaagtt
    40741 ttaaattgag gacaaagaat gattgaatac aaaaatgtag cactgcgcta cacagaaaag
    40801 gatgtcttga gagatgtcaa cttacagatt gaggatgggg aatttatggt tttagtaggg
    40861 ccttctgggt caggtaagac gaccatgctc aagatgatta accgtctttt ggaaccaact
    40921 gatggaaata tttatatgga tgggaagcgc atcaaagact atgatgagcg tgaacttcgt
    40981 ctttctactg gttatgtttt acaggctatt gctctttttc caaatctaac agttgcggaa
    41041 aatattgctc tcattcctga aatgaagggg tggagcaagg aagaaattac gaagaaaaca
    41101 gaagagcttt tggctaaggt tggtttacca gtagccgagt atgggcatcg cttacctagt
    41161 gaattatctg gtggagaaca gcaacgggtc ggtattgtcc gagctatgat tggtcagccc
    41221 aagattttcc tcatggatga acccttttcg gccttggatg ctatttcgag aaaacagttg
    41281 caggttctga caaaagaatt gcataaagag tttgggatga caacgatttt tgtaacccat
    41341 gatacggatg aagccttgaa gttggcggac cgtattgctg tcttgcagga tggagaaatt
    41401 cgccaggtag cgaatcccga gacaatttta aaagcgcctg caacagactt tgtagcagac
    41461 ttgtttggag gtagtgttca tgactaattt aattgcaact tttcaggatc gttttagtga
    41521 ttggttgaca gctctatctc aacatttgca gttgtcgctt ttgaccttgt tactagctat
    41581 tttgcttgcg attcccttgg ctgtttttct tcgctatcat gagaagctgg ccgactgggt
    41641 cttgcagatt gcaggtattt tccagaccat cccgtctctg gccttgttgg ggctctttat
    41701 ccctttgatg ggaattggga ccttgccggc tttgacagct ctagtgattt atgcgatttt
    41761 ccctattttg caaaatacta tcactgggct gaagggaatt gatccgaacc tgcaagaggc
    41821 tgggattgcc tttgggatga ccagatggga acgtctcaag aaatttgaaa ttccactcgc
    41881 catgcctgtt atcatgtctg ggattcggac ggcagctgtt ttgattatcg gtacggcaac
    41941 cttggcggcc ttgattggtg cagggggact aggttccttt attcttttgg gaattgaccg
    42001 taataatgcc agtttgattt tgattggggc actttcttct gcagtgctag ccattgcctt
    42061 taacttccta ctaaaagtga tggaaaaagc aaaattacgg acgattttct caggttttgc
    42121 cttggtggct ttattactgg gtctgtctta tagtccagct cttttggttc aaaaagagaa
    42181 ggaaaacttg gttattgctg ggaaaatagg tccagaacca gaaattttgg ccaatatgta
    42241 taagttgctg attgaagaaa ataccagcat gactgcgact gttaaaccga attttgggaa
    42301 gacaagcttc ctttatgaag ctctgaaaaa aggcgatatt gacatctatc ctgaatttac
    42361 tggtacggtg actgaaagtt tgcttcaacc atcacccaag gtgagtcatg aaccagaaca
    42421 ggtttatcag gtggcgcgtg atggcattgc taagcaggat catctagcct atctcaaacc
    42481 catgtcttat caaaacacct atgctgtagc tgttccgaaa aagattgctc aagaatatgg
    42541 cttgaagacc atttcagact tgaaaaaagt ggaagggcag ttgaaggcag gttttacact
    42601 cgagtttaac gaccgtgaag atggaaataa gggcttgcaa tcaatgtatg gtctcaatct
    42661 caatgtagcg accatggagc cagcccttcg ctatcaggct attcagtcag gggatattca
    42721 aatcacggat gcctattcga ctgatgcgga attggagcgt tatgatttac aggtcttgga
    42781 agatgacaag caactcttcc caccttatca aggggctcca ctcatgaaag aagctcttct
    42841 caagaaacac ccagagttgg aaagagttct taatacattg gctggtaaga ttacagaaag
    42901 ccagatgagc cagctcaact accaagtcgg tgttgaaggc aagtcagcaa agcaagtagc
    42961 caaggagttt ctccaagaac aaggtttgtt gaagaaatga tgggacagag actttaaaca
    43021 tactgaactc aacttcctta aacgactaga taaaagaatg ctcagacaat ttcgtctggg
    43081 cttttttgat gtgagaatga ctgaatgtca cttaaaatgg aaagaaagag aaaattttca
    43141 tgattttcta aaattttact gtaaagacag ttgactattc ataaaatagg tgtataatat
    43201 tttgtgaact acttaacaaa taagaggttt cagctcatgt cgactaaggt tagaaatctt
    43261 gtgtataggc agttcattat ctaatagagc gttgcgtccg aaagtctatc cagacacggc
    43321 tctttaaaaa caaaaggaga aatgatgcat acttatttgc aaaagaaaat tgaaaatatc
    43381 aaaacaaccc taggtgaaat gtcaggtggt taccgtcgta tggttgcggc tatggctgat
    43441 ttaggatttt caggaactat gaaggctatc tgggatgacc tctttgccca tcgtagtttt
    43501 gcccagtgga tttatttgct ggttttagga agttttcctc tctggctgga gttggtttac
    43561 gaacatcgta ttgttgactg gattgggatg atttgtagct tgacagggat tatctgtgta
    43621 atctttgtat cggaaggtcg agcaagtaat tatctttttg gcttgattaa ctctgttatt
    43681 taccttattt tggccctaca gaaaggcttt tatggtgagg tgctgacgac actttacttc
    43741 acagtcatgc agccaattgg acttctagtt tggatttatc aggcacagtt taagaaggaa
    43801 aagcaggagt ttgtcgcgcg taaactggac ggcaagggct ggacaaagta tctttccatt
    43861 agtgtgcttt ggtggttggc ctttggcttc atttatcagt ctattggtgc caatcgtccc
    43921 tatcgtgatt caatcacaga tgcaaccaat ggggtagggc aaatcctcat gacagctgtt
    43981 taccgtgaac agtggatatt ctgggcggct accaatgtct tttcaatcta tctctggtgg
    44041 ggagaaagcc tgcaaattca agggaaatat ctaatttatc tcattaacag tctagttggt
    44101 tggtatcaat ggagcaaggc agctaagcag aatactgatt tacttaacta ggaaaagatg
    44161 tttgaaagtg ctgttttgag atttcgatta aaacagatat agttgataat caaggattta
    44221 tagtatgaaa aagaggatcg gcgggtcctc ttttgttgtt gaaaagataa aaaactcagt
    44281 aacctagaaa taagacaact gaagctttac tctatattca atttttagga atgagaaggt
    44341 ctagataaaa ttggacaact tcctggtctg tgaaatcttg accttttttg agccaccagg
    44401 tcaatgtctc gataaagttg gacatgacca agtgttggag gtaagaagta ggcagattag
    44461 ggtgggcttc ttttaaatta tcagctagca cggaatagac atggtgttct agctctttat
    44521 ggagttgacg gaggaagtag tcatttttgg aaaatagcag actggtgata tggtcttggt
    44581 ttttatgaaa atggagaaag aggtgggcga ggtagtcctc ggttgaaatg gcttgctctc
    44641 tttcaaaaag atgatggaag aggtagcggc agagctggtc cagaagaagc tccttactct
    44701 catagtgaca gtaaaaggtg gatcgtccca catctgcgag atcaatgata tcctgaacag
    44761 tagtggcctc gtagccctta gcattcaaaa gttgtataaa agcttgatag atggcttttt
    44821 tggttttgct gatacggcgg tcaatgttag tcatatggac acttaaggca aattgttcag
    44881 aactgaataa agctgacgtt ttgcttctat cctttctttg agttttagtg gataatgata
    44941 atgaacaagg tgttcataaa tctattataa caaaggaatg agaaatatga aggcaaaata
    45001 tgctgtttgg gtggcttttt tcttaaattt gacttatgcc attgttgagt ttattgcagg
    45061 tggagtattt ggttctagcg ctgttcttgc tgactctgtg catgacttgg gagatgcgat
    45121 tgcaattgga atatcagctt ttctagaaac aatctccaat cgtgaagaag acaatcagta
    45181 caccttgggc tataagcggt ttagcctgct aggagccttg gtaacagctg tgattctcgt
    45241 aacgggctct gttctagtca ttttggaaaa tgtcacgaag attttgcatc cgcaaccagt
    45301 caatgatgag gggattctct ggttaggaat tattgcgatt actatcaatc tgttagcgag
    45361 tctggtggtt ggtaagggaa agacaaagaa tgagtctatt ctgagtctgc attttctgga
    45421 agatacgcta gggtgggtag ctgttatcct gatggcgatt gttcttcgat ttacggactg
    45481 gtatatccta gatcctcttt tgtcccttgt catttctttc tttattcttt caaaagccct
    45541 tccacgtttt tggtctacac tcaagatttt cttggatgct gtgccagaag gtcttgatat
    45601 caagcaagta aagagtggcc tggagcgatt ggacaatgtg gccagcctta atcagcttaa
    45661 tctctggact atggatgctt tggaaaaaaa tgccattgtc catgtttgtc taaaagaaat
    45721 ggaacatatg gaaacttgta aagagtctat tcgaattttc ctaaaagatt gtggttttca
    45781 aaatattacc attgaaattg atgctgacct agaaactcac caaacccata agcgaaaggt
    45841 gtgtgacttg gaacggagtt atgagcatca acattagaaa aaagtgaaaa atacttgggt
    45901 actatcttat ttggaataga gtaatttctt tattatttaa atatttcaaa aattggtaag
    45961 agaagagcat tgtataaact ccagatatat gattgttaat gataaaaatt tttcgattag
    46021 atacaaaatg cttgacttgg agtcaactca aagttatata ataagataag tgagttagaa
    46081 tagcgtgaat tcagtgaatg aaatgagagg aggttagcgt gtgaatatta aatctgccag
    46141 tgatttgttg ggaatttcag cggatacgat tcggtattat gaacgggttg gtcttgtgcc
    46201 accgattact cgtactgcta ctgggattcg tgattttcaa gatcaggata tcgaagcgct
    46261 ggaatttatt aagtgttttc gttcggcggg tgtctctgta gatagtttag ttgactatat
    46321 gtcgctctac caaaagggag atgaaacgag agaggagagg cttggtattt tagaagagga
    46381 aaagcaaaaa ttagaggagc gcttgtctca gctacagaca gctttaaatc gtttaaatct
    46441 caaaattaaa ctttataagg aaggaaaatt ttaaatgaaa tcagcagtat atacaaaggc
    46501 aggtcaggtt ggacttgcta gcattgaacg tccgcaaata atagaagcgg atgatgtgat
    46561 tattcgtgtg gttcgtgcgt gcgtttgtgg ttcagattta tggaggtacc gtaatccaga
    46621 aacgaaagct ggacacaaaa atagtggaca cgaagcgatt gggattgttg aagaagctgg
    46681 ggaagccatt acgacggtga aaccaggtga ttttgtgatt gtccctttta cacatggatg
    46741 tggtgagtgt gatgcctgtc ttgctggatt tgacggttct tgcgacaatc atattggcaa
    46801 taatttgggg ggtgattttc aggcagaata tattcgcttc cactatgcaa actgggcgct
    46861 ggttaaaatc cctggtcaac cttctgacta tacagaaggg atgctccagt cccttttgac
    46921 tcttgcagat gtcatgccga catgctatca tgcggcgcgt gttgcaaatg ttcaaaaagg
    46981 ggacaaggtt gttgttatcg gtgatggggc tgttggtcaa tgtgctgtca tcgcggctaa
    47041 gatgcgtgga gcatcacaaa ttatccttat gagccgtcat gaagaccgtc aaaagatggc
    47101 tatggagtca ggtgcgacag ctgttgttgc agaacgtggt caagaaggaa ttaccaaggt
    47161 gcgtgaaatc ctcggtggag gagcagacgc agcacttgaa tgtgttggta cggaggctgc
    47221 tatagaacag gcgctaggtg ttcttcataa tggagggcgt atgggctttg taggagtccc
    47281 acactataat aatcgtgctc ttggttcgac atttatgcaa aatatctctg tagcaggtgg
    47341 ggcagcttct gctacaacat acgataagca atttttacta aaagccgtcc ttgatggtga
    47401 tatcaatcca ggtcgcgtct ttacttcaag ttataaactg gaagatatcg accaagccta
    47461 caaagatatg gatgaacgta agacaattaa gtctatgatt gtaatcgaat aaaaaacgaa
    47521 taggagtttt agaactctat tcgtttttta tgttatccta ttcttgattt agggtacttt
    47581 ctcttaatgt cagtctggtt cccagcatgg tcaggctagg aattttgcga ccgtggagga
    47641 cttccttgtt aagaatatcc atacctgctc gccccatttc ttcagtataa actgtaatac
    47701 tagagagggg aggatagacc tgtttggtca gactggtgtc gttgaatgaa atgaggctga
    47761 cgcgatctgg caggctgatt ccagcttctt ggagggcacg gagggcaccg atagctaaac
    47821 tatcgctggc tgcgaaaaat gctggcggaa gttggtctcc caagctctga atggcctcct
    47881 tcattaagtc atagccagac tgggcagtaa atcttccttg aaagaccagt tcatcatgat
    47941 agattcccct cgcttgactg tagtttttga agttttctag acgcttgtcc tgaatgattt
    48001 cttcttggtc tgttgtttcc tcaagccctg ttagaatccc gatgcggtcc atcccttgac
    48061 tgaggaaata atcgacaacc tgtttcatag cagtgtaaaa atccgtgata atacaggtat
    48121 gtcccaggga aagtgtatcg ctgtctagaa atacaagagg cttttggtat tcttcaaagg
    48181 cagaaatctg agctcggcta aattttccga tgcagagaat cccaatcact tcctcgctta
    48241 aggtaaaagg atggtcatta aaatagcgca agatatcgta gtccaactct tgggctcttt
    48301 tttctattcc taggcgaatc tggtagtagt agaggtcgtc tagctcccct tgttcgctga
    48361 cccattggat aatggcaatc ttttgcttgg gcttgtggga ctcgcctgtc ttgagatgtt
    48421 tggtgtagcc tagctcttca gcaacggtta aaatacggtg tctggtttct tctgtaacag
    48481 ataggctctg gtcgcggttg aggacacggg atacggtcgc gattgagaca gaggctagct
    48541 gtgcaatgtc ttttaaggta gccataaatc ctccttgatt aggttagtat atcatatttt
    48601 tcttcttttt actgatattt tagtaaaatt ttagtaaaaa ggattgacct tgggaaattc
    48661 cttggataca atagaaagaa aacgattaca cgttaagatg gcttaacgga cagtcaaagg
    48721 agaattcata tggcacaaca tcttactact gaagtccttc gcaaagactt tcttgctgtt
    48781 tttggtcaag aagcagacca aaccttcttt tcaccaggcc gtattaattt gattggtgaa
    48841 cacacagact acaacggtgg gcacgttttt cctgctgcta tttccttggg aacttacggt
    48901 gcagctcgta agcgtgatga ccaagtcttg cgtttctact cagctaactt tgaggacaag
    48961 ggcattatcg aagtgcctct cgctgacctc aagtttgaaa aagagcacaa ctggaccaat
    49021 tatccaaaag gtgtccttca tttcttgcaa gaagctgggc acgtgattga caaaggtttt
    49081 gatttttatg tttatgggaa tatcccaaat ggtgctggtt tgtcttcttc agcatccttg
    49141 gagctcttga caggggtagt ggcagagcat ctctttgatt taaaattaga gcgtctcgat
    49201 ttggttaaaa tcggaaaaca aacagaaaat aactttatcg gagtcaactc tggtatcatg
    49261 gaccagtttg ctattggtat gggggctgat caacgtgcta tttaccttga taccaacacc
    49321 ttagagtatg atttggtgcc acttgatttg aaggacaatg tcgttgttat catgaacacc
    49381 aacaaacgcc gtgaattggc ggactctaaa tacaatgaac gtcgtgctga gtgtgaaaaa
    49441 gcagtggaag aattgcaagt tgccttggat attcagaccc tgggtgaatt ggacgagtgg
    49501 gccgttgacc aatatagcta tttgattaaa gatgaaaatc gtttgaaacg tgctcgccat
    49561 gctgtgcttg aaaaccaacg tacccttaaa gctcaagcag cccttcaagc aggtgatttg
    49621 gaaacatttg gtcgtttgat gaatgcgtca cacgtttctc tggagcatga ttatgaagta
    49681 actggtttgg aattggatac tcttgttcac acagcttggg cacaagaagg agttctcggt
    49741 gctcgtatga caggggctgg ttttggtggc tgtgccattg ccttggttca aaaagatact
    49801 gttgaggcct ttaaggaagc tgtaggcaaa cactacgagg aagtagttgg atacgctcca
    49861 agcttctata tcgctgaagt tgcaggtggg actcgcgtcc ttgactagtc aaaaggaggc
    49921 tctatagtga ccttagtaga taaatttgta acacatgtca tttctgaaag ttcatttgag
    49981 gaaatggatc gaatctacct gaccaatcgt gttttggcac gagtgggaga aggtgttttg
    50041 gaagttgaga ccaatctgga taaattgatt gacctcaagg accagctagt tgaggaagcc
    50101 gttcgattag agatgattga ggatagtcag actgcgcgtg aaatccttgg tactgaactg
    50161 atggacttgg tgactccttg tccgagtcag gtcaatcgtg acttctggga agcctatgcc
    50221 tactctcctg agcaagcgat agaggatttt tatcaactca gtcggaaaaa tgactacatc
    50281 aaactcaagg ccattgctaa aaatatcgct tatcgtgttc catctgatta tggagaactt
    50341 gaaattacca tcaatctctc taagcctgaa aaggatccga aagagattgc ggtagccaag
    50401 ttggtgcaag ctagtaatta tcctcagtgt caactttgtt tagagaatga gggttaccat
    50461 ggtcgggtca accacccagc tcgcagcaac caccggatta tccgttttga aatggttggt
    50521 caggaatggg ggttccagta ttcgccctat gcttacttta atgagcattg tatcttttta
    50581 gatggccagc atcgtcccat ggccattagt cgtcagagtt ttgaacgtct gttggctatc
    50641 gtagagcagt ttccaggata ttttgctgga tctaatgccg acctgccgat tgtggggggc
    50701 tctattctaa ctcatgatca ttatcaggga ggccgtcacg tatttcctat ggaattggct
    50761 cccttgcaaa agaccttccg atttgctggt tttgagcagg tcaaggctgg aattatcaag
    50821 tggccaatgt ctgtcctacg cttgacttcg gattccaaag aggatttgat taacttagct
    50881 gataagattt tccaggaatg gcgtcagtat tcagattcta gtgtgcagat tttagcagag
    50941 acagacggga caccgcatca caccatcaca ccaatagcgc gtaagcgtga tggacagttt
    51001 gagttggact tggtcttgcg agacaatcag acttcagcag agcatcctga tggcatctat
    51061 catccccaca aggatgtcca acatatcaag aaggaaaata tcggcttgat tgaggtcatg
    51121 ggcttggcaa tcttgccacc acgtctgaaa gaagaagtgg agcaagtcgc tagctatctt
    51181 gtaggagaag ctgttacagt tgccgattat catcaggagt gggcagacca actcaaatcc
    51241 caacatccag accgaacgga taaagaaaaa gcccttgcaa tcgtcaagga ctctgtgggt
    51301 gctatctttg tgcgtgtact tgaggatgca ggagtctaca agcagacaga acaaggtcag
    51361 acagccttta tgcgctttgt ggaacaggtc ggaattttac tagactagga gctttctcct
    51421 tgcacagtcc tataaaaagt agtatcatag tgtcgtttga aatatcagga ggaaacgatg
    51481 aaagactttc attttgacgc tatatctgcc tttgaaaatt acgaaattga aaaaatgaga
    51541 gatggtcacg ttgtggtgac gaccaaagta gtgaactcgt cgctcaacta ctatggcaat
    51601 gcccatggtg gctatctatt cacgctatgt gaccaaatca gtggtttggt ggttatctcg
    51661 ctgggacttg atggggtgac actccaatcc tctatcaact accttaaggc aggaaaactc
    51721 gacgatgtat tgaccattaa aggagaatgt gtccatcaag gtcgtacaac ctgtgtaatg
    51781 gatgtggata tcaccaatca agaaggcaga aatgtctgca aagcaacctt taccatgttt
    51841 gtcacaggcc agcggtcaga agacagacag gtaaggatat aaagagtaat ttaatagagt
    51901 gaggtgaatt ttggaattta taagatttgc cttactcatt ttttgatatt gatatgattt
    51961 aattctaaaa tagaaaattt agtaatgtgg tagaaaatac aagagttgtg ttttaatttc
    52021 tatggtataa ttaaaagcat gaagataaaa gaaataaaga aagttacttt acaaccgttc
    52081 acgaaatgga caggtggtaa aagacaatta ttgcctgtta ttagagaatt aatacctaaa
    52141 acctataaca ggtatttcga accttttgtt ggaggtggag ctttattttt tgatttggct
    52201 cctaaagatg cagttattaa tgattttaac gctgaactaa taaattgcta tcaacaaatt
    52261 aaggacaatc ctcaagaatt gattgaaatt ttgaaagttc atcaggaata taattcaaaa
    52321 gaatattatt tagatttacg ttctgcagat cgtgatgaaa gaatagatat gatgtccgaa
    52381 gtacaaagag ctgcacgtat tctatatatg ttgagagtga actttaatgg tctatatcgt
    52441 gtgaattcta agaatcaatt taatgttcca tatggacgtt ataagaatcc taaaattgtt
    52501 gatgaggaat tgatatctgc tatttcagtt tatataaata acaatcaact agaaattaaa
    52561 gtgggagatt ttgaaaaggc aattgtagat gttcgaacag gagattttgt gtattttgac
    52621 cctccatata ttccattgtc tgagacgagt gcatttacgt cttatactca tgagggattc
    52681 tcttttgcag atcaagtaag attaagagat gcctttaaga gattgagtga tacaggagct
    52741 tatgttatgt tatcaaattc ttctagtgct ttagtagagg agttgtataa ggattttaat
    52801 atacattatg ttgaagctac ccgaactaat ggagcaaaat cttcaagtcg aggaaaaatt
    52861 tctgaaatta tagtcacaaa ttatgaaaaa taacgaatat aagtatggag gtgttcttat
    52921 gacaaaacca tactacaata aaaataagat gattcttgtt cattcagata cgttcaagtt
    52981 cttatcaaaa atgaaaccag aaagtatgga tatgattttt gctgatccac cttatttttt
    53041 aagtaatggt ggaatatcta attctggggg acaagtagtt tctgttgata aaggagattg
    53101 ggataaaatt tcttcattcg aagaaaaaca tgagtttaat cgtaaatgga ttcgcctagc
    53161 aaaagaagtt ctgaagccta atgggacggt atggatttca ggtagtttgc acaacatata
    53221 ctcagttgga atggcattag aacaagaagg ttttaaaatt ctgaataata ttacttggca
    53281 gaaaacaaac cctgccccca atttatcttg tcgttatttt acccattcta ctgaaaccat
    53341 tttatgggcc agaaaaaatg ataaaaaagc tcgtcattac tacaattatg atttaatgaa
    53401 agaattgaat gatggaaaac aaatgaaaga tgtctggacc ggttctttaa caaagaaagt
    53461 tgaaaaatgg gctgggaaac atccaactca aaaaccagag tatttgttag aacgtattat
    53521 tttagcctct actaaagagg gtgactatat tctagaccca tttgttggta gtggcactac
    53581 gggtgttgtt gcgaagcggt taggtagaag atttataggt atcgatgctg aaaaagaata
    53641 tttaaaaatt gcaaggaaga ggttggaggc agaaaatgaa acaaactaga aattttgatg
    53701 agtggttgtc aacgatgacc gacacagtag ctgactggac ttattataca gattttccta
    53761 aggtttataa aaatgtatct agtataaaag ttgctctaaa tattatgaat agtcttattg
    53821 gtagtaaaaa tatacaagag gattttttag acttatatca aaattatcca gaaattttga
    53881 aagttgtgcc tctacttatt gctaaacgtt tgagagatac gattattgtt aaggatccga
    53941 taaaagattt ctattttgat tttagtaaac gaaactatag tattgaagaa tatactatgt
    54001 ttttggaaaa atcaggtata tttgatttat tgcaaaatca tttagtatca aatttagttg
    54061 actacgttac aggtgttgag gttggtatgg atactaatgg gcgcaaaaat cggactggag
    54121 atgcgatgga aaatattgtt caaagctatc ttgaagctga agggtatatt ttaggagaga
    54181 atttatttaa agagattgaa caaaatgaaa tagaggaaat tttttctgtc gatttatctg
    54241 ctattacgaa tgatgggaat actgtgaaac gttttgattt tgtcataaaa aatgaacaag
    54301 tgctttatct gattgaggtt aatttttatt caggaagtgg ttcaaaatta aatgagacgg
    54361 ctagatctta caaaatgatt gctgaagaaa ctaaagctat tcctaatgtt gagtttatgt
    54421 ggattactga tggacaaggt tggtataagg ctaagaataa tttacgagaa acatttgata
    54481 ttttaccatt cttatataat attaacgatt tggaacataa tattttaaag aatttgaaat
    54541 aggtccaatt caattagatg ggaattggga ttgattgtat ttataatcaa tttgtgataa
    54601 acaaaaaaga ggctcgcacc tctttttctt atttcttttt atgatttaat acggcattga
    54661 ggacaatggc gagtaggctg gctacgacga ttccgtttga gaagaacatt tggaaggctg
    54721 tcggcatgct gacaaagaga ttactgttgt tgagaccgac acctgcagcg attgaaacag
    54781 ctgcgataag gaagttgtgt tcattgttag caaagtcaac tcgggcgagg atttgcatcc
    54841 cttgaataga tacaaaacca aacatcacca gcatggcacc gccgaggacg gagcttggaa
    54901 tgatttgggc aagggcacca aacttaggaa gcagtccaag gagaaccagg aaaccagctg
    54961 cgtagtagat tggcaggcgt tttttgatgc ctgacaattt aaccaaacca acgttttgtg
    55021 aaaatccggt gtaagggaag gtgttaaaga ttcctccgag aagtaccgcc aaaccttctg
    55081 cgcggtaacc gttgcgaagg cgcgtgctgt cgattggatc ttttgtgata tcagacaagg
    55141 ctagataaac accagttgac tcaaccatag acaccgttgc gatgatacac atcatggcaa
    55201 tagatgagat ttcaaaggtt ggcatcccaa agtagagtgg agttgggaca tggacaagtg
    55261 gagctaccgc aacaggagag aagtccacca agcccatagt agcagcaatg gcagttccaa
    55321 caaccagacc aatcaaaata gagatagact tgataaatcc tttggtaaag atgttgatca
    55381 agaggataat cagaacagta atagctgcaa gcaagagact ttgaccagtt ggctctggaa
    55441 cgttatttcc catatttcca atagcgacag ggatcaaggt taaaccaatc gtggtaataa
    55501 cagatcctgt tacgatagat gggaagagat tggctacttt tgagaagatg cctgaaacaa
    55561 gaaccacgta aatcccagat gcgataaggg caccaaacat agcgccacta ccatggcttt
    55621 gcccaatcat aatcaaggga gcgaccgact ggaatgcaac tccaagaacg actgggagtc
    55681 caatcccaaa gtatttgttg agttggagtt ggaggaaggt tgccacccca cacatgaaga
    55741 tatctgtaga aatcaggtag gtcaactgct cagctgaata gccaagggct gtcgcaatca
    55801 tgatgggaac caggatagat cctgagtaca tggctagtaa gtgctgcaag cccagaacgg
    55861 ctgcttgcga gtgtttttct tgagtttgca ttagagatct gcctccttaa atacgacctg
    55921 accattttca aaacgatcca agcgagcaag tgataggaca gggtagcctg ctttttcaag
    55981 caaatcacga ccatcttgga aggatttctc aatcacgata ccgatagctt ggactgtggc
    56041 accggcctgt tcgatgattt gaatcaagcc tttagcagct tggccattag caaggaaatc
    56101 gtcgataatc aaaaccttgt cctctggtga gaggaatttt ccagcgatag aaacagtgct
    56161 ggtcacctgc ttggtaaagg agtagacttg agcagttaag atgccttcgt tcatggtgat
    56221 gttcttagct tttttggcga aaatcatggg aacgtttaag gcttcagctg taaaaacggc
    56281 tggggcaata cccgacgctt caatggttac gaccttggta atgccagcag cagcaaattt
    56341 ttccgcaaaa accttaccaa tctctcgcat caagctaaag tcaacttggt gggttaaaaa
    56401 ggaatctacc ttgaggatgt tatcacccaa gatatgccca tccttgagga tgcgctcttc
    56461 taataatttc ataagacctc ctaaagtcta aaagttaatt tacttgttgt ttaaatgttt
    56521 ctatagtgat cccttttgct aatactatat atttgataaa actattacga gcgaagcgag
    56581 tcttatcaaa tatttcccgt tgtagtggta tcatagacaa taatcttgtt attgtctatg
    56641 acgggatttt tgagagtaaa atagttcggg gaactatttt agcctaagcc tagaaatgaa
    56701 agagctaggg gctcaaaaat tagggatgaa attccctgga ttcctgaaat tattcacagg
    56761 ataatttcac ctcccgtccg cactaattaa gggaaatatt aaaaatattc aaaaaagacc
    56821 tacttaatct ctaagtaagt cccccaaata ggcatggcaa aaacggccat atctcactgc
    56881 tgacttactt attgttaggt gttccggcac cttgtagaaa cgtcgtgcca attcacgaca
    56941 taaacaagta aaacgatatt caattttaaa taggtttgag ccaatgtttt tattttacac
    57001 taaataactt tataaatcaa ctattttgtt agtgttttgg tttaaaaaac gaacaaacag
    57061 aagaaggagt gaacaaaaac tgcattgtaa gctaacattt atactaaatg aaaatcaaag
    57121 agcaaactag gaagctatcc acaacctcaa atcactgttt tgaggttgtg gatagaattg
    57181 acagagtcag tatcatatac ctacggtaag gcgacgttga tgtggcttga agagattttc
    57241 gaagagtatt agaagatttt tccatcataa aaggcatact atcaagtttt taaacacctg
    57301 acaatatgcc tttttctaac tttaaagact tttcccaatt tttattattc tactcgctaa
    57361 atcttaaaaa atagccattt ggatccaaaa ctgcaaattt atgaggatag atatagggat
    57421 cactgacacg aaactttctt ttggtcaagg gacgataaat aggatagttt gccttcatca
    57481 ctctttaata gagttttgaa acatccttta tgccaaagga gagattgact ccacgaccaa
    57541 aggggtaggt cagttcagct agttgatcct ttgttccctc ctctaacatt agttgacact
    57601 cttcaagaga aagaaagaga aagttttctt ctggacgttg gtattcaatc ctaaaaccca
    57661 gtaaaccaca gtagaaggac cgagactgtt cgatattcga tacaagcaac tcgggaatga
    57721 ccgcattgta gtccatatag aaaatcctta caagtcaatt tccaagacaa tcggtgtatg
    57781 gtcttggcga gcacctgagt caatcatatc agatttagtg accttgtcag cgatacggtt
    57841 acttgtgagc cagtagtcga ttctccagcc tgtattgttg attttagaag ttttgctgcg
    57901 ttgtgcccac caagtgtagc gttcaggaac atcgccatga acatggcgga aggtgtctgt
    57961 aaatccagtt gccaaaaggt tggtaaatcc agcacgttcc tcgtcagtaa atccaggtga
    58021 acggcggttg ctagcaggat ttgcaaggtc gatttcattg tgggctacgt tgtagtcacc
    58081 ggtcgcaagg actggttttt ctttgtctag ttcagccaaa tactcagcat atttaacatc
    58141 ccagacttgg cgttcttcca agcgtttgag accgtcacca gcgtttggag tgtaaacttg
    58201 ggttacgaaa aatgcatcaa attctagagt gatgatacgg ccttccaagt ccatggtaga
    58261 aggggcaccg atttctggga agctgatcgt aggcgtaagt tctttcttat aaaggaacat
    58321 ggttccagca tagcctttac gggcaggctc ttgggaagag cgccacgtgt tttcgtagcc
    58381 tgggaagagt tcttctaaaa tttccacgtg tttctttgta ggtcctttgg cagaaagctt
    58441 ggtttcttgg atagcaataa tatcagcatt ttcagcgacc aaggtttgta ggacttcttg
    58501 ggacaatttg gcacgagctg agtcactagt tagggcagcg tttagggaat caatattcca
    58561 tgagataagt ttcataaagt tacctttttc attcagatta tagattttat tataccaaaa
    58621 aaagatctat ttccccaacg tatggtttga aaaattactc tctttcgttt ataattaaga
    58681 atgattttat gaaagggagt gaaaatacat gaaattctac tcttatgact atgtactcag
    58741 ccaaatcggt cagcaaaatg gtatcatggt tggctttggg attgttctat tagctgtgac
    58801 agtttttttg ctttcaaggc ataccatgat aaaaaaggaa ccaaatttcg tgagttggtc
    58861 atgatttcag ctctggcctt atttagctct gcttttggtc agcatcacga cttatcaaaa
    58921 caatcaagtt tctaacaata aatttcaaac ttcacttcat ttcatcgagg ttgtttccaa
    58981 agatttggga gtagacaagt cagaagtcta tgttaatact tccacaaaca aagatggcgc
    59041 acttatcaag gtgggagatc gctattatcg tgccctaaac ggaagtgagc cagacaagta
    59101 cctgttagag aaagtcgaat tgtataagac agacgcaatt gaactggtgg atgtgaacaa
    59161 atgacactta attatatcga aattttaatc aaactggtct tgactctcaa atagctcaac
    59221 aacaatgttc actttgtgaa acgtttgatt gatggtaagc caactctcct tatcaaaaat
    59281 gggaatattg acccagaagc ctgtcgttca gttggtttgt ctgcatcgga tgtagccctc
    59341 aaacttcgta gtcaagagat tttccagatg aagcaagtca aacgagctgt gcaagagcaa
    59401 aatgggcaac tcatcgttgt gcaaatggga gatgaaaatc ctaagtatcc agttgtgact
    59461 gacggtgtga ttcaagtaga tgtcttggaa tcgattggtc gtagcgaaga gtggttgctt
    59521 gataacctca gtaaacaagg gcatgacaat gtagccaata tctttattgc tgaatatgac
    59581 aagggtgctg ttacagtcgt aacttatgaa taagaaaaac ctggggtctt gtactcttcg
    59641 aaaatctctt caaaccgcgt caacgtcgcc ttgccgtatg taggttactg acttcgtcag
    59701 ttctatctac aacctcaaag cagtgctttg agcagcctgc ggctagtttc ctagtttgct
    59761 ctttgatttt cattgagtat tggcctcagg tttccatttg caatcagaaa gggattttat
    59821 gtccattatt caaaaacttt ggtggttttt caagttagaa aaacgccgtt atctagtcgg
    59881 gattgtggcc ctggtcttgg tttccgtcct caatctcatt cctcctatgg ttatggggcg
    59941 ggttattgat gccatcacat cggggcaatt aacccagcag gacctccttc ttagcctatt
    60001 ttacttgcta cttgcagcct ttggtatgta ctatttgcgc tatgtgtggc gtatgtatat
    60061 ccttgggacc tcttattgct tgggacagat catgcggtct cgcttgttta agcatttcac
    60121 aaaaatgtcg tcagcctttt atcaaaccta tcggacgggt gatctgatgg cacacgcaac
    60181 caatgatatc aatgccttga ctcgtttagc aggtggcggt gtcatgtctg cggtggatgc
    60241 ctctatcacg gctctggtga ctttgttgac catgctcttt agcatctcat ggcagatgac
    60301 tcttgttgcc attctccccc tacctttcat ggcctatacg actagtcgcc tagggagaaa
    60361 gactcataag gcctttggcg aatcccaagc tgctttttct gaactcaata acaaggtaca
    60421 ggagtccgta tcaggtatca aagtgaccaa gtctttcggt tatcaggcag acgagttgaa
    60481 gtcttttcag gcagtcaatg aattaacctt ccaaaagaac ctgcaaacca tgaaatatga
    60541 tagtctcttt gaccctatgg ttctcttgtt tgttggttcg tcctatgttt taacgctttt
    60601 ggttggctcc ttgatggttc aggaagggca gattacagtt gggaatctag tcacctttat
    60661 cagctatttg gatatgctgg tctggcctct tctggccatc ggtttcctct ttaatactac
    60721 tcagcgaggg aaggtttctt accagcggat tgaaaatctt ttgtctcagg aatctcctgt
    60781 acaagaccct gagtttcctc tggatggtat tgaaaatggg cgtttggagt atgccattga
    60841 cagctttgct tttgaaaatg aggaaacact gacggatatt cactttagtt tggcaaaagg
    60901 gcaaacactg ggcttggttg ggcagacagg ctctgggaaa acgtccttaa tcaagctcct
    60961 cttgcgtgaa tacgatgtga ataagggtac tatttatcta aacggtcacg atattcggga
    61021 ctatcgtctg acagaccttc gcagtctcat gggctatgtt cctcaggacc agtttctttt
    61081 tgcgacttca atcctagaca atatccgctt tggcaatcct aacttgcccc tttcagcggt
    61141 cgaggaagct actaagctag cccgagttta ccaagatatt gtagacatgc ctcaaggatt
    61201 tgatacgctg attggtgaaa aaggagtcag tctttctggt ggtcaaaagc aacggttggc
    61261 tatgagtcgg gctatgattt taaaccctga tatcttgatt ttggatgatt ccttatccgc
    61321 cgtagatgcc aagacagagt atgcgattat cgacaacctc aaggagacgc gaaaggacaa
    61381 gacaaccatt atcactgccc atcgcctcag tgctgttgtc catgcagatt ttattttagt
    61441 tctacaaaat ggtcaaatta tcgaacgagg caggcacgaa gacttgctag cgatggatgg
    61501 ctggtatgcc caaacctacc agtctcagca gttggaaatg aaaggagaag aagatgcaga
    61561 ataaacaaga acaatgggtt gtattgaatc gcttgatgtc ttatctcaag ccttatagac
    61621 tcctgacctt tttggcactc agttttctcc tagcgacgac ggtcattaaa agtgtcatac
    61681 ccctcgtggc ttcccacttt atcgaccagt atctcagcaa tcttaaccaa ctagccgtta
    61741 ccgttttgct ggtctactat ggtctctaca tcctacaaac tgtagttcag tatgtaggca
    61801 atcttctctt tgcgcgcgtg tcttacagta ttgttaggga tattcgtcga gatgcctttg
    61861 ccaatatgga gaaactgggc atgtcttact ttgacaagac gccagcaggt tctatcgttt
    61921 ctcgtttgac caacgatacc gagacgatta gtgatatgtt ttctgggatt ttatccagct
    61981 ttatctcagc agtttttatc tttctgacaa ccctttatac catgttggtg ctggattttc
    62041 gtttgacggc tttagtcttg ctctttcttc ctttgatttt ccttttggtc aatctctatc
    62101 gaaaaaagtc agtgaaaatc atcgagaaaa ccagaagtct cttgtcagat atcaatagta
    62161 agctggcaga gaatatcgag ggaatcagga ttattcaggc ttttaatcaa gagaagcgcc
    62221 tgcaggcaga atttgatgaa atcaaccaag agcacttggc ctatgccaac cgttctgtag
    62281 ccttggatgc cctctttttg agacctgcca tgagtttgtt gaaacttcta ggttatgctg
    62341 tcttgatgac ctattttggc tatcgtggtc tttctatcgg gataacggcc gggaccatgt
    62401 atgcttttat ccagtacatc aaccgcctct ttgacccttt gattgaggtg acgcaaaact
    62461 tttcaacact gcaaacgtct atggtttctg caggccgtgt ctttgcctta atcgatgaga
    62521 ggacctatga gccccttcaa gaaaatgggc aagccaaagt caaagaaggc aatatccgtt
    62581 ttgaacatgt gtgtttctcg tatgacggta aacatccgat tctggatgac atttctttct
    62641 ctgttaataa gggtgaaacc attgcctttg taggtcatac aggttcaggg aaatcgtcta
    62701 ttatcaatgt cctcatgcgc ttttatgaat tccagtcagg gagagttctc ttggatgatg
    62761 tggatatcag ggatttcagt caagaagagc tgagaaaaaa catcggtttg gttttacagg
    62821 aacccttcct ctatcatgga actattaagt ccaatatcgc catgtaccaa gaaatcagtg
    62881 atgagcaggt tcaggctgcg gcagcctttg tggatgcaga ttcctttatt caagaacttc
    62941 ctcaggggta cgactcccct gtttccgagc gtggttcgag cttctctact gggcaacgcc
    63001 agcttcttgc ctttgctaga acagtcgcca gccagcctaa aatcctgatt ttggatgaag
    63061 cgacagccaa tattgactct gaaacagaaa gcttggttca agcttctctg gcgaagatga
    63121 gacagggccg aacaactatt gctatcgctc accgcctttc tactattcaa gacgccaact
    63181 gcatctatgt cttggataag ggacgcatta tcgagagtgg aacccatgag gaactcttga
    63241 ctctgggagg aacctatcac aagatgtata gtttgcaggc aggggccatg gcctatactc
    63301 tttgaaaatc tctttaaacc atgtcagctt tatctgcaat ctcaaagctg tactttgatt
    63361 ttcattgagt actagaagga aatccttcaa attacagatt tctttcaccg ccttttccat
    63421 tttgtggtat aatgaaaaat gttgacaaat agtataataa aaacaaagga gaacagcatg
    63481 ctgaaatggg aagacttgcc tgtggaaatg aaatcaagcg aggttgagtc ttactaccag
    63541 cttgtctcta aaaggaaggg ttcgctgatt ttcaagcgtt gcttggactg ggttttggcc
    63601 ttggtcttac tggttctgac ctctcccatc tttctcatct tgagcatttg gatcaagttg
    63661 gatagcaagg ggccagtgat ttacaagcaa gagcgtgtga cccagtacaa ccgtcggttc
    63721 aagatttgga agttccgtac catggtgacg gatgcggata aaaaaggaag tctggtgact
    63781 tctgctaacg atagccgtat taccaaggtt ggaaatttca tccgacgtgt ccgtttggac
    63841 gaactgcctc agttggtcaa tgtccttaaa ggtgagatgt cctttgtcgg tacacgacct
    63901 gaagtgccac gttatacaga gcagtatagc cctgaaatga tggcaacctt gctcttgcaa
    63961 gcaggaatta cctctccagc cagcatcaac tacaaggatg aggacaccat catcagtcaa
    64021 atgacggaga aaggtctgtc agttgatcag gcctatgtgg agcatgttct tcctgaaaag
    64081 atgcgctata acctcgccta tctccgagag tttagttttc ttggggacat caaaatcatg
    64141 tttcaaaccg tgtttgaggt actaaaataa agtagtcata agaaaatgag tacagataaa
    64201 aggagcaaat caatgccaaa ttacaatatt ccattttcac cgcctgatat cacagaagca
    64261 gaaattgctg aagtagcgga taccctgcgt tctggttgga tcacaacagg tcctaaaaca
    64321 aaagaactgg agcgctgctt gtctctttac acacagacac ctaagactgt ttgtctcaac
    64381 tctgcgacag ctgctctgga gttgatttta cgcgttttgg aagtgggacc tggtgatgaa
    64441 gtcatcgttc cagccatgac ctatacggct tcatgtagtg tcattacgca cgtgggagca
    64501 acccctgtca tggtggatat ccaagcagat acgtttgaga tggactatga cctgcttgag
    64561 caagctatca ctgagaaaac taaggtgatt atcccagtag agctcgcagg gattgtttgc
    64621 gattatgacc gtttgttcca agtcgtggag aaaaaacgtg acttctttac cgcttcaagc
    64681 aagtggcaaa aggcctttaa ccgtattgtc attgtctctg atagtgccca cgctttggga
    64741 tctacttata aaggacaacc ttctggttct atcgctgact ttacttcctt ctcattccat
    64801 gccgttaaga actttacaac ggcagaaggt ggaagtgcga cttggaaagc caatccagtg
    64861 attgatgacg aagagatgta caaggaattc caaatccttt cccttcacgg gcaaactaag
    64921 gatgctcttg ccaagatgca attggggtca tgggaatacg atatcgttac accagcctat
    64981 aagtgcaaca tgaccgatat catggcttca cttggtttgg tacaattgga ccgctatcca
    65041 agtttgttgc aacgccgtaa ggacattgtg gaccgctatg atagtggttt tgcaggttct
    65101 cgcatccatc ctttggcaca caagactgaa actgtcgaat cttcacgcca cctctacatc
    65161 acccgtgtag aaggagcaag cctagaagaa cgcaacctca tcatccaaga attggctaaa
    65221 gcaggaattg caagtaatgt tcactacaaa ccgcttcctc tcttgacagc ctataagaat
    65281 cttggatttg atatgacgaa ctatcctaag gcctatgcct tctttgagaa tgaaattacc
    65341 ctccctcttc atactaaatt aagcgatgaa gaagtagact atatcattga gactttcaaa
    65401 acagtttctg aaaaagtgct aactttatca aaaaaatgac aaactacagt caagcgaaag
    65461 tgatcctgtc cctaaaaagt ctaattgagt gtaaaaactg ttgttttcaa ttgataatag
    65521 tttacacctg tagttgaggc ctctttctcc tcagagagag aatttttata ggattttcct
    65581 ttcttgtggg agtcccgtgg tttgaaataa gatgtgagca atttggtata gcatttagaa
    65641 tccttactag acatcattta gaaaatctag tgtcttgttc tagttttcaa ttcaccctat
    65701 tttttgaaag acgtgagttt ccatgagtga gattgtggaa actagcgtct tttttttgtt
    65761 ttcagaatat tgttcaaagt tttgtgcctg tctttcatgt tctagtcatt cttttgcatg
    65821 atagaattta tagcatgttg atattataac aacgcaaata tcccatatgt ttagtgatgc
    65881 ttgctataca tcattagatc tcctgcgaga caatctataa aacacttgtc tacgattacc
    65941 tatatgccct attccagtat tttagaagca ctgcatctat ttttatcgag gttaaatcta
    66001 gcttttatag aaggtctatt taagaaatat attgtagtgt tttagtttca atccgccata
    66061 tgagcgatat tcatgtaaat atccctggcg aatgcttgta tgacaagata tttgttcttt
    66121 catt
//
LOCUS       DAVKJJ010000005        53271 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000005 DAVKJJ010000000
VERSION     DAVKJJ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 53271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53271
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.005"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            33..848
                     /locus_tag="VW144_000296"
     CDS             33..848
                     /locus_tag="VW144_000296"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEU9599796.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            867..1508
                     /locus_tag="VW144_000297"
     CDS             867..1508
                     /locus_tag="VW144_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEU9599797.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRREEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            1539..2540
                     /locus_tag="VW144_000298"
     CDS             1539..2540
                     /locus_tag="VW144_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU9599798.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEKV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            2550..3179
                     /locus_tag="VW144_000299"
     CDS             2550..3179
                     /locus_tag="VW144_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU9599799.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHTETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
     gene            complement(3558..3943)
                     /gene="tnpA"
                     /locus_tag="VW144_000300"
                     /pseudo
     CDS             complement(3558..3943)
                     /gene="tnpA"
                     /locus_tag="VW144_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596946.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(4327..7530)
                     /locus_tag="VW144_000301"
     CDS             complement(4327..7530)
                     /locus_tag="VW144_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEU9599800.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKGG
                     AITISSHEKLRAAVHRMVPIEAKKKYKLRFKIKTDHKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTARLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYYWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVGRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            7753..8229
                     /locus_tag="VW144_000302"
     CDS             7753..8229
                     /locus_tag="VW144_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEU9599801.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQAQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRIEWNFSKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            complement(8451..10664)
                     /locus_tag="VW144_000303"
     CDS             complement(8451..10664)
                     /locus_tag="VW144_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEU9599802.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSIPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(11065..11241)
                     /locus_tag="VW144_000304"
     CDS             complement(11065..11241)
                     /locus_tag="VW144_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9599803.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            11388..11810
                     /locus_tag="VW144_000305"
     CDS             11388..11810
                     /locus_tag="VW144_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9599804.1"
                     /translation="MRFIVETFPIFSISNDLLQLLTSWKKQQKYELAKFGIISNPEQF
                     IFTYIDTKGNINKPLHSDYLNNKMKTIRKRHPELTHATPHKLRHTGATLAKQAGMSLE
                     AISEALIHSDTGTTQIYVNTSNVVPMAVGEFALQSLKQ"
     gene            complement(12071..12463)
                     /gene="rpsI"
                     /locus_tag="VW144_000306"
     CDS             complement(12071..12463)
                     /gene="rpsI"
                     /locus_tag="VW144_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU9599805.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(12482..12928)
                     /gene="rplM"
                     /locus_tag="VW144_000307"
     CDS             complement(12482..12928)
                     /gene="rplM"
                     /locus_tag="VW144_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU9599806.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            13285..13602
                     /locus_tag="VW144_000308"
     CDS             13285..13602
                     /locus_tag="VW144_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEU9599807.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(13752..14564)
                     /gene="folK"
                     /locus_tag="VW144_000309"
     CDS             complement(13752..14564)
                     /gene="folK"
                     /locus_tag="VW144_000309"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU9599808.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(14607..15161)
                     /gene="folE"
                     /locus_tag="VW144_000310"
     CDS             complement(14607..15161)
                     /gene="folE"
                     /locus_tag="VW144_000310"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU9599809.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(15142..16464)
                     /locus_tag="VW144_000311"
     CDS             complement(15142..16464)
                     /locus_tag="VW144_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9599810.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLMGGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALAVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEEISWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(16466..17440)
                     /gene="folP"
                     /locus_tag="VW144_000312"
     CDS             complement(16466..17440)
                     /gene="folP"
                     /locus_tag="VW144_000312"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU9599811.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGADLVNDITGLMGDEKMAHVVAEARAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEDLMVAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASTIRLADEAENLDLKQYK"
     gene            complement(17535..18242)
                     /locus_tag="VW144_000313"
     CDS             complement(17535..18242)
                     /locus_tag="VW144_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9599812.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGLVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            complement(18295..19713)
                     /locus_tag="VW144_000314"
     CDS             complement(18295..19713)
                     /locus_tag="VW144_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU9599813.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLVITLTKVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            19903..20715
                     /locus_tag="VW144_000315"
     CDS             19903..20715
                     /locus_tag="VW144_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9599814.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKRTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            21054..22073
                     /gene="adhP"
                     /locus_tag="VW144_000316"
     CDS             21054..22073
                     /gene="adhP"
                     /locus_tag="VW144_000316"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEU9599815.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLTLAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            22306..23295
                     /gene="manX"
                     /locus_tag="VW144_000317"
     CDS             22306..23295
                     /gene="manX"
                     /locus_tag="VW144_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEU9599816.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            23323..24126
                     /locus_tag="VW144_000318"
     CDS             23323..24126
                     /locus_tag="VW144_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEU9599817.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            24150..25061
                     /locus_tag="VW144_000319"
     CDS             24150..25061
                     /locus_tag="VW144_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9599818.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(25117..26451)
                     /gene="pepC"
                     /locus_tag="VW144_000320"
     CDS             complement(25117..26451)
                     /gene="pepC"
                     /locus_tag="VW144_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEU9599819.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALECRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(26763..26993)
                     /locus_tag="VW144_000321"
     CDS             complement(26763..26993)
                     /locus_tag="VW144_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9599820.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            complement(27003..28244)
                     /locus_tag="VW144_000322"
     CDS             complement(27003..28244)
                     /locus_tag="VW144_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEU9599821.1"
                     /translation="MVLPNFKENLGKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFKNAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            complement(28266..28418)
                     /locus_tag="VW144_000323"
     CDS             complement(28266..28418)
                     /locus_tag="VW144_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9599822.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            complement(28439..28717)
                     /locus_tag="VW144_000324"
     CDS             complement(28439..28717)
                     /locus_tag="VW144_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEU9599823.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            complement(28710..28973)
                     /locus_tag="VW144_000325"
     CDS             complement(28710..28973)
                     /locus_tag="VW144_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEU9599824.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            complement(29075..33466)
                     /locus_tag="VW144_000326"
     CDS             complement(29075..33466)
                     /locus_tag="VW144_000326"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEU9599825.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPAFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFESVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            complement(33570..35651)
                     /gene="fusA"
                     /locus_tag="VW144_000327"
     CDS             complement(33570..35651)
                     /gene="fusA"
                     /locus_tag="VW144_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU9599826.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEEIRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            complement(36076..36546)
                     /gene="rpsG"
                     /locus_tag="VW144_000328"
     CDS             complement(36076..36546)
                     /gene="rpsG"
                     /locus_tag="VW144_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU9599827.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            complement(36566..36979)
                     /gene="rpsL"
                     /locus_tag="VW144_000329"
     CDS             complement(36566..36979)
                     /gene="rpsL"
                     /locus_tag="VW144_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU9599828.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            37396..41238
                     /gene="spuA"
                     /locus_tag="VW144_000330"
     CDS             37396..41238
                     /gene="spuA"
                     /locus_tag="VW144_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEU9599829.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPGNANASLEKTEEKPAASPA
                     DPAPQTGQDRSSEPTTSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDD
                     VEKPSENWPNGALSFKDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVE
                     KLVPKMNEAWLDQDYKVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQW
                     PDGTDFTATGKYGRYIDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQ
                     IFLKDDDESIYTNPYYVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHL
                     GNKVTITDVAIDEAGKKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGK
                     LGADLKEEGKQVDLTLWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDST
                     NKLGITDFTGYYYQYQIERQGKTVLALDPYAKSLAAWNSDDSKIDDAHKVAKAAFVDP
                     AKLGPQDLTYGKIHNFKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDY
                     LKDLGVTHIQLLPVLSYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSD
                     PKNPEKRIAEFKNLINEIHKRGMGAILDVVYNHTAKVDLFEDLEPNYYHFMDADGTPR
                     TSFGGGRLGTTHHMTKRLLIDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARAL
                     NPNLIMLGEGWRTYAGDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPA
                     FITGGKRDVNTIFKNLIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKA
                     ENYAEIHRRLRLGNLMVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHL
                     LRDKDGNPFDYPYFIHDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQS
                     TDAFRLKSLQDIKDRVHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKARE
                     FNLGTAFAHLRNAEVLADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTS
                     HESTAEEKPDSTPSKPEHQNEASHPEHQDPAPEARPDSTKPDTKVADAENKPSQATAD
                     SQAEQPAQEAQASSVKEAVRNESVENSSKKNIPATPDRQAELPNTGIKNENKLLFAGI
                     SLLALLGLGFLLKNKKEN"
     gene            complement(41338..42387)
                     /locus_tag="VW144_000331"
     CDS             complement(41338..42387)
                     /locus_tag="VW144_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU9599830.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAVHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGSNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            42444..42581
                     /locus_tag="VW144_000332"
     CDS             42444..42581
                     /locus_tag="VW144_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9599831.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEVVDRTDEISNHTYGKATLTWFEEI
                     F"
     gene            complement(42658..44466)
                     /gene="glmS"
                     /locus_tag="VW144_000333"
     CDS             complement(42658..44466)
                     /gene="glmS"
                     /locus_tag="VW144_000333"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU9599832.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKKALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(44658..46037)
                     /gene="bglA"
                     /locus_tag="VW144_000334"
     CDS             complement(44658..46037)
                     /gene="bglA"
                     /locus_tag="VW144_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU9599833.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIHDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KKLSDNNGF"
     gene            complement(46137..47990)
                     /locus_tag="VW144_000335"
     CDS             complement(46137..47990)
                     /locus_tag="VW144_000335"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU9599834.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(48003..49262)
                     /gene="rseP"
                     /locus_tag="VW144_000336"
     CDS             complement(48003..49262)
                     /gene="rseP"
                     /locus_tag="VW144_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU9599835.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(49284..50087)
                     /locus_tag="VW144_000337"
     CDS             complement(49284..50087)
                     /locus_tag="VW144_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU9599836.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMYLFGLF"
     gene            complement(50096..50854)
                     /locus_tag="VW144_000338"
     CDS             complement(50096..50854)
                     /locus_tag="VW144_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU9599837.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            complement(51085..51549)
                     /locus_tag="VW144_000339"
     CDS             complement(51085..51549)
                     /locus_tag="VW144_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU9599838.1"
                     /translation="MAAGSVRNFIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLE
                     KKLREDFPQYQWELKNQVYMHQHSPHTASYTSSRDAMSKYPERCTAVGLRLNEESDFE
                     LYAPYGLEDILNFQVRPTPHFLENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(51638..52609)
                     /gene="ruvB"
                     /locus_tag="VW144_000340"
     CDS             complement(51638..52609)
                     /gene="ruvB"
                     /locus_tag="VW144_000340"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU9599839.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            52770..52862
                     /locus_tag="VW144_000341"
     CDS             52770..52862
                     /locus_tag="VW144_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEU9599840.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
BASE COUNT        15587 a        12127 c        10078 g        15479 t
ORIGIN      
        1 gaagtcttaa caaatttgaa aggaagtatc aaatgacaaa tacatcattc tcaattgagc
       61 agttttcttt aaaaggaaaa attgctctca tcaccggcgc ttcttatgga attggatttg
      121 ctattgccaa atcctacgct gaagccggcg ctactattgt ctttaacgat atcaatcaag
      181 atctggtcaa taaagggatt gaagcttatc gtgaagttgg catcgaagcc catggatatg
      241 tctgtgacgt gacagacgag gacggtatcc aagccatggt caagcaaatc gaacaagagg
      301 ttggtgtcat tgacatcctc gttaataacg ctggtattat ccgccgagtt ccaatgtgcg
      361 aaatgagcgc cgctgatttc cgtaaggtca tcgatattga cttaaacgca ccatttatcg
      421 tttcaaaggc agttattcct tctatgataa agaaagggca tggaaagatt atcaatattt
      481 gttcgatgat gagcgaactg ggacgtgaaa cagttagcgc ttatgctgct gctaaagggg
      541 gcttgaaaat gttgacccgc aacattgcgt ctgaatacgg tggagccaat atccaatgta
      601 acggaattgg accgggttat attgccactc ctcaaacagc acctcttcgt gagttgcaag
      661 aagatggttc tcgccaccca tttgaccagt tcatcattgc aaaaacacct gctgcacgtt
      721 ggggaaatcc tgaagatttg atgggccctg ctgtctttct cgctagtgat gccagcaatt
      781 ttgtcaatgg ccacatccta tatgtagatg gcggtatctt agcctacatc ggaaaacaac
      841 ctgagtaaaa atagaaagaa gatcttatga aaatcgcatt aatcaatgaa aatagtcaag
      901 ctagcaagaa tcacattatt tacgatagtc taaaagaagc gacagataaa aaagactacc
      961 aattatttaa ctatggtatg cgtagagaag aaggagaaag tcaattaact tatgtgcaga
     1021 acggactaat ggctgccatc cttttaaata caaaggcagt tgactttgtt gttaccggct
     1081 gtggtacggg tgtaggggct atgcttgctt taaacagctt ccctggtgtt gtctgtggtc
     1141 tagcagtgga cccaactgac gcttaccttt attctcaaat caatggtggt aacgccttgt
     1201 ctatccctta tgccaaagga tttggctggg gggcagaact gaccctcaaa ttgatgtttg
     1261 aacgcttatt tgctgaagaa atgggcggtg gctacccaag agaacgtgta atccctgaac
     1321 aacgtaacgc tcgtatctta aacgaggtga aacaaatcac ccacaatgat ttgatgacca
     1381 tccttaaaac aatcgaccaa gacttcctca aagacaccat ctctggcaaa tacttccaag
     1441 aatacttctt tgaaaactgc caagatgatg aagtcgctgc ttatttgaaa gaagtattag
     1501 ccaagtaaag ctattctaaa ccagaaagga actaatggat gacgaaaata ttactgtttg
     1561 gcgaaccatt aattcgaatc tcaccattag atgccaccag tatcggcgat catgttgcca
     1621 gttcgactta ttttggcgga tcagaaatta acatcgcttg taatttgcaa gccctgggta
     1681 tctcaacgaa agtctttacc gcactccctg ccaacgagat tggagatcgt tttctcacat
     1741 tcttgaaaca gcaccaaatc gataccagtt caatctgtcg gcttggcgat cgaatcggcc
     1801 tctactattt ggagaacggc tttggttgtc gtcaaagtga agttttctac gatcgtaagc
     1861 atacgagtat cagccagatt cggccaaaca tgctagatat ggattctctc tttcagggga
     1921 ttagccattt tcattttagt ggaatcaccg tagctatcgg tcaagaggtc cgtgcgatcc
     1981 ttctcctact cttggaagaa gccaagcgcc gaggaattgt cgtttcaatg gatctcaatc
     2041 tgagaacaaa gatgatttca gtcctagaag ccaagtatga attttctaag tttgcacgtt
     2101 ttactgacta ttgcttcggt attgatcctc tcatgattga tgaccaaaat ctagagatgt
     2161 ttccaagaga cagtgctagc ctagaaaagg tggaaaatcg catgcgactt ttaaaagaag
     2221 cctatggttt caaggccatt ttccataccc tccgctctag tgatgagcaa gacaaaaatg
     2281 tctatcaagc ctatgctcta gaagaacgat ttgaagagtc tgtccaacta aaaactgcag
     2341 tctatcaacg aattggtagc ggggatgcct ttatatctgg tgccctttac caactactcc
     2401 atcattcctc cctaaaaact accattgact ttgcagttgc gagcgcaact ctcaaatgca
     2461 ctcttccagg agaccatctc tccacttccg caactagtat tgaaaattta ctggcaaatg
     2521 cacaagatat cattcgttag gagaattaca tgaccaaatc agatacaatt attgaactaa
     2581 aaaaacagaa aattgtcgct gtcattcgag gaaatacaaa ggaagaagga ctacaagcct
     2641 cgattgcttg tatcaagggc ggtatcaaag ctattgaaat cgcctatacc aatcagtatg
     2701 caggacaaat catcaaggaa cttgtagact tgtatcagga cgatcagagt gtttgtatcg
     2761 gtgcaggtac tgtgcttgat gccgtaaccg ctagagatgc cattctagcc ggagcaaatt
     2821 acgttgtttc tccatctttc catactgaaa ctgcgaaaat gtgcaatctc tacagcacac
     2881 cgtacattcc aggctgtatt accctcacag agatcacgac tgcacttgaa gccggtagtg
     2941 aaatcatcaa actcttccca ggtagtactc tcagtccagc atatatctct gcagtcaagg
     3001 caccgatccc acaagtttcc gtaatggtaa ccggaggagt cggtctaaac aacatccctc
     3061 aatggttcgc tgctggtgca gacgccgttg gaattggtgg cgaactcaat aaactcgctt
     3121 cccaaggcaa ctttgaccgc atcagcgaga ttgcccaaca gtatgttaca ctcagataaa
     3181 atcataacca cccgtcaaac gggtggttta tcccagagct ataagcccaa attaccagcc
     3241 agcgtctaaa gacgctggct tttacgttgt tcaagcctta ttgctcttga ctcgtcactt
     3301 gcctctttaa gagactttgg tattacttac cactatccct aaagggatcc tcatattctt
     3361 ttacactcaa tttatctagt gctatagtag attgaaactg gaatagtaca cctctgcttc
     3421 taaaacattg ttagaaatcg atttgactgt cctgatcgat tttgtcctgt tcttatttca
     3481 ttttactata tatcatgctt tactcgttct caaattttca tactcgtgaa gaaatcatcc
     3541 actggataat ttctttaatc ttgactatat ttcttaattg tggctttatt aagccctact
     3601 gtacttacaa aataaccttc cgcccagaaa tgccgattcc caaacttgta cttgagattg
     3661 gcgtgtttgt caaacatcat gagtgcactt ttgcctttta aataccccat aaaacttgaa
     3721 acacttagcc ttagtggaat actgactaac atgtgtacat ggtctggcat taagtgaccc
     3781 tcgatcattt caacaccttt ataactacac aagcgatgaa atatttcgcc caaactactt
     3841 ctatattgat tatagatgac ttttcgtcta tacttagggg tgaacacaat atgatagaac
     3901 acatccactt tgtgtatgat aaactatgag tcttttgtgc catatttttt ctcccttcgc
     3961 tttacaagtg gagtgaacac ctttattgta tcgtgtttgg agtttttttg gtataacctt
     4021 cgacgcgcac ccgcatagcg ggtggttgtt ttgtctcgca cctcacggag cgagacggac
     4081 ttatatagtg gagtgaaata ggatacgaac aaattgatta ggaaaatcaa atgaatttat
     4141 agaaatcttt tagcagttat aacgttctat tctagtttca aaacgctata gtcacataat
     4201 aatgaagtaa aaaaggataa gtatcaactt atcctttttt aaaagaaaaa tccgaagata
     4261 tttggccttc ttcggatttt ttctattttc cacagtttca tgtaattcat ctagatgatg
     4321 aacaaattag ttgttctttc ctctacggaa taaataaaac gccccaagta gcaagaaccc
     4381 tagacttgcc aagattgact gaccttctcc tgtctgaggg agattctttt gatccgaatg
     4441 gttcttttcc tcttcagatt tttccttttc ttttgaattc tgtacttgtg gctgagctgc
     4501 ttgctctagc tttttaaaga cttcctgatc tggagctgat tcctgggttt caggattata
     4561 gtaggcaatc ttatattcat ccccttcttt tcgaatggta tagactccac gtttcaaaac
     4621 ttggaattgg ttggaaatag tagagacaga atcatcatat ttcacaatgc cccaaactcc
     4681 ttgtttggca tcataaacag actgaagggt ttcgttattt tcgatgaggc tactttctaa
     4741 ctcttttatc atttgattga aggtggcacg acccacgtta gggatgagca tatagccata
     4801 agaatctcca ttttgcttat gagcctgact aatcgtaaga aattcatttt caacttcctt
     4861 gtctgactgt ccttcattga tatccttcca ggctcccttt tgcaaagcct tactcatact
     4921 gattgaactc ttcttaaaga aaaagtaacc aatattcttt ttcgaatcgg atgattctaa
     4981 aaagacactt tgggtttcag gataatcctt ttcttgttct gtaagggagg cttctttatc
     5041 attgacatag actttatatg gattacttga ttccagtttt ctctggtcaa ttgtagttgc
     5101 agcagtatct gttgaagtgt tttggatatt gcttcctaaa aaggcgatct tatcctttag
     5161 cataaaccag ctcttatgag cagtcaatgt ttgattccag ttggtgaaat ccatggttgc
     5221 tgtcgcattg gcatcatcta gtttgctcgt tccaacgaaa gcagacggta aaactttacc
     5281 tgtatcgcta tccgctctct tagcatccgt ctctgttgta ccaggcatct tatatggatt
     5341 aactgttggc cagtagccat cgctatagtg actcaaatcg ccattgtaaa gatagaacat
     5401 cccatcactc gtataccaac cacgtttatt ttccttgttc atgtgttcgt aattcaaggt
     5461 acgactggaa aagagtgaca agccaaatcc aaaccctttc tctgcattgt acatggctgt
     5521 tttatccatc ttgttaaagg cagataggta acttgttctt ggaacacttg cgactcctgc
     5581 atcacttaac aaggattgca tcaaactgat atccttataa gtcttcaaat tcttaaagac
     5641 atcataataa ctatccgatt gaacaatggt cttcacaaga ctctgcaaac gttgtttggt
     5701 ttctccttca gacatatccg ctattcggtg aatccctctt agtacttcta ctgcggccac
     5761 gtgcccctcg ctatttgcac gactgatcga gcgtccacga ctcatatcca tcagctctcc
     5821 attcaccagc aaaggagcaa acgatttatc aatccagtag tacatggttt gcattttatc
     5881 tttatcgatt ggattcttgg tcttttgaat gactggcaac agttgagaca ggccatcaat
     5941 caaaacattc ccataagcac ccgtataggc aacattggta tggtcgatat aggatccatc
     6001 ttgataaaaa ccttcacctt ggtctaccaa cttgaacact tgctcaatcg agcgaatggt
     6061 agaagaaatt tcttgatcat ccttacgcag taaaccagct attactttta ctcttcccat
     6121 atcaactaag tttccaccta gagccttaaa tgggttatca gtcgtctttc ggaaatgttc
     6181 gggatctggt acaaattttt caatcacatc tgtatatttt ttaatttcct catcagagaa
     6241 gtattctttc atcagagaca aggtattgtt gatggcacga ggtgtaccga tttcataatc
     6301 ccaccagttc ccaacaatgc tcttttcact attgtagaca tgtttatgca tccattccat
     6361 ggaatccctg actgttcgaa cgacagtttc atcttgataa taacgagaag aaggattggt
     6421 cacttgcttg gccatctcct ccaatttccg ataagtggca gtcagatttg cagacatttt
     6481 ataatttgaa aatttttccc acaaataggt gcggtccgcc tgacttgaaa tactagatag
     6541 gctatcagct acctttcctt ccaattcctg gtttaatttg gccatctgtt catttttaga
     6601 atcatagtat tgattcccag cgatgatgcc attccagtca tccaaacggg ctgtgtatgc
     6661 atccttaaca gaggctagaa tcttcaaagg aatctttttc acttccttgc catctttact
     6721 gacaatgaca ttggttgtcc cttccttaag aggttctaaa attccatttt tgactgaagc
     6781 aacgtcagga ttttctacct tataagtata gtccgcaaga gaaaaaacat gtttttttcc
     6841 aattggtaaa tcaatctttt cctcaagctg tttatctgtt tgagaatcct cagaaagctg
     6901 gtctgctacc tctaccagct caatatcctt aaaggaaaca gtcccagttc ctgtttcata
     6961 gaataactcc agcttgattt tatcaacatc taaagtcggg ctatagtctg cttcaatggt
     7021 ctgccagtcc tttgttcctg acgtcgttgc agaattccac aatcgcttgt ccttaccact
     7081 ttcctcaatg atacgaacct tggcaatccc gactttatga tctgttttaa tcttgaaacg
     7141 cagtttatac tttttcttag cttcaatagg aaccatacgg tgaaccgctg cccttaattt
     7201 ctcatggctt gagatagtga tagccccacc cttagcctca atgactcgag ttgaggcatc
     7261 tgcagaacta ttcttctggt ctacccaagc tgaccacccc tgagctttgc ttcctgtcca
     7321 cggactgctt ccgtcctccg tctgaccaaa atcaccattt tcaactaaat tttttgtatc
     7381 cctttgctga tactgaactt ccttctggct ttgctgaatg gtattcgtag tcgtttcttc
     7441 cgctccaact gtcatataat ggtttaataa aaatcctgat aaaacaagtg aagacaaact
     7501 cacaatgagc ttctttgttt ttgtttgcat gtatacactc caataaacat attgtaaaat
     7561 caataggaac taaatctcaa attagagatt tatagcaact aagtaaaccg gtttaattaa
     7621 atcataccat ttttaaagtt atttgtaaac ggtttctatc atattgaaga cttattcttc
     7681 aacaatatct tccatccgac tcgacgaaaa aagtaaaaac tgttacaatg tagaaaaaga
     7741 aaagaggccc tcatgacttc actttatgat ttttccgttt tgaaccaaaa caaccaagca
     7801 actcccttgg atagctatcg tggcaaagtt ctcttgattg tcaacactgc tactggatgt
     7861 ggtttaacgc cccagtacca gggacttcaa gaactctatg aacgctatca agctcagggc
     7921 ttcgaaatat tggatttccc ttgcaatcag tttatgggac aagcacccgg cagcgcagag
     7981 gaaatcaacg ccttctgtag cctacatttt caaaccacct tcccacgttt tgccaagatt
     8041 aaggtcaacg gtaaggaagc agaccctctc tatgtctggt taaaagacca gaaatccggc
     8101 ccactaggaa aacgaatcga atggaatttc tctaagtttc tcatcggtcg agatgggcaa
     8161 gtctttgaac gcttttcttc aaaaacagac ccaaaacaaa ttgaagaggc gatacaaact
     8221 ctactataat tcacaatctc actatgatta ggtttccttt aacctgatga atagtgagat
     8281 tttttgatgg gctttgactt aaatagaaaa acaccccatg atataaaaca tgaagtgttg
     8341 taaagtctat gttgtaggtg cttatttcac attttcaatg tgaccagtga taacgaatac
     8401 catacagaat cttcatatac actaaacaaa tgactttcta attatttcaa ttagttttgg
     8461 ctagtaaata tcatttccaa caaacgccct ctcaattcct tatcctgatg atgcaagata
     8521 ttcattaagt catgagagtt tttcgcattg atgaattgat ttaacaatct atcttttaat
     8581 tcatatggaa gagaagctgt ctttagtagt ctaaaaactt cgtcatttaa agatgtcctt
     8641 ttattatctt tccattcaaa tttagctgta tcattcttat ttggcaattc aattatagac
     8701 acattcgttc ctttaaaatg aattctatgt tttctattgc ttggaacgat actagaatct
     8761 ccttgtaatg ctaactctac cattcccatt tcccaatcga ttgataatct tgttttatat
     8821 ctttgaccat tttgatcttc aagcatttca aaagaatgtt gttttcctgg gaatacatac
     8881 caatctacaa cttcaggtaa atcaacaccc atacctatct cagaaccaac caagggaatg
     8941 attgcaccac tttttgcaaa cacaggcgta gtcgagatgt ctctataaac acttaacttc
     9001 acaccacctg tgtatttttt ctctgaaaaa aagtcatacc attcaccttc agggaaccat
     9061 acatctactt ttgcagattg gaatgtcaaa tccatctttt ctacaatggg agccaccatc
     9121 agttctgttc caaaaaagta ttgatttgga acattatagc tctcatcatt ctctggatag
     9181 aaataataga ttggactgat taatggggca ccttcctcat gtgtctttac attcatggta
     9241 tatagataag gaatcatctg atgtctcaaa cgaaggtatt tcttcataat cttagatgtt
     9301 gtttctgaaa aaaaccaagg ttctttacta ttaaaaggac ttctagaact atgtaatcga
     9361 gtaatcggac taaaaacacc aaactgtagc catctagttt gtagctcttc gtcataatcc
     9421 cccaacatat gtccaccgat atcatgactc caccaactat aaccgatatt agatgctgtc
     9481 gctgtaaaat agggttgaaa tcttaaggaa ttccaactaa taatagtatc ccctgaaaaa
     9541 ccaacagggt agcggtgact accaggacct gcatatcttg ataaaatcaa accaccttct
     9601 gcatttttac aactatcctg atagtgataa tggtttaaaa gccaaagtgg atctagcata
     9661 ccttgtgtcc cttgttgcca gtcaatccac caaaaatcta ctccctgctt ttctagctca
     9721 taatgaacat ctttaaagta ggcttcccta aaagagggat taaaaaaatc aaaaatagca
     9781 ggttcttcta gttctacatt taaccccaat cgttttgcga tttgaggata agcttcttca
     9841 taagcccgta tcccatcagc aggatggaca tttaaggaga gttttaactt tctatcatga
     9901 agttgttgca ataactgttc tggatttggt attaagtttc tattccaact atatcctgtc
     9961 cagccacttc caaaacgagc tggaatgtca gttatatgcc aatccatatc taacacaccg
    10021 atagataatg gaattttctc tgtttcaaat ctgtctatta aatccaagta ttcatccgac
    10081 gtataaggcc aatatctact ccaccaattg cctaaagcat atcttggcaa caagggtatt
    10141 gaaccagtca aatggtaaaa gtctctgatt gctcctctat aatcatgccc ataggcaaag
    10201 aaatacaggt caatttgatt ttctctctca atataaccag attgttcatc ccaaataaat
    10261 ccttgagaat catccaataa ggctatacca tttcggctaa taattccatc ttctaacgag
    10321 attgctccat ctgccttatc cagagtccga gctgttcctt ttaacgtttc aatagattca
    10381 ccgaaatacc agcgactacc atatacggca aaatttcctt ttaattctat aaataaattt
    10441 tcggcgttaa attctccttt attaaagtgc agatgaaaat agtccgtcat aatatctagt
    10501 acatttgatg tctcgatata atctaacgaa acttggccaa aatctctatt atagataagt
    10561 tgtgtcgttc tatcctcaaa atttccagtt cgagagtatt ctaaccttac tagcttgtct
    10621 gttaatacag agattcgata aaactctccc ttaaaaattt tcaatttgtt ttcctccttt
    10681 tatggtagca taaaaacaga acgcaccatt tttgatgcgt ttttcattat tctgaatgca
    10741 atgttctatc tgttatatct atgacaaata atagtcaatt gaaaaaatgc agtggacaaa
    10801 atatctttta acaaaccaag agtttattaa agagttatca cttttcaact tttctaagct
    10861 tatgcagttg tgaaacaaac tacttttaaa ctattaacta agataggatt gataaataat
    10921 ttcaaactct tactagcaat catacgatat tcaagctcac gtgctttttt ccttcctgct
    10981 tatttcttag aactgaagaa cccggatcgg tatataaatt atcaggatca acatagtcat
    11041 aagactcata acagttgcgt ttcattaagt catccccaga gcaagagctt catctcgtaa
    11101 tttttcaaca tcactaaccg taggtcgcca tccttcaatc atatttgtac ttaaagcata
    11161 ccaaacactc ttaaaaacgg atcggttttc aaaagctatt cccatgattg tcatcttttc
    11221 tttatctata tctaaggaca tatgctacct cctttagata cattatacca tgtttctatg
    11281 tagcttttaa aaattttatt ttgtttgtca tatctaagtt ttcagcacgc ttatcctatt
    11341 ttataagcct caaacccaaa tataaaacgc attaaccatc tgataatatg cgttttattg
    11401 tggaaacatt tcccattttc tctatttcta atgacttact tcaacttctg acttcatgga
    11461 aaaaacaaca aaaatatgaa ctggcaaagt ttggtatcat cagtaatcca gaacaattca
    11521 tctttactta catagatacc aaaggcaata tcaataaacc tttacattct gactatctca
    11581 ataataagat gaaaactatc agaaaacgac acccagagct tacacacgct acgccacaca
    11641 aactacgtca taccggagca acgctcgcta aacaagcagg aatgagctta gaagccattt
    11701 ctgaagctct aatacatagc gacacaggta caacacagat ttatgtcaat acttctaatg
    11761 tagtccctat ggccgtcggt gaatttgcct tacagtctct aaaacaataa ggtgaaaata
    11821 aggtaaactt tgaggtgaac tttttcaaaa aacataaaaa aagcacccat gaggtaaact
    11881 cttgagtgct ttgaaacgtt gatataattg tattgattaa cgttttgagg caactgacct
    11941 tgtcaggttg cagccacatt tgtaagcgac taaagtcgca acaactgtgt caattgcacc
    12001 aatttagtaa gaaagtataa aaaagaacac cccgaaaggt gctctgtata agtatagtaa
    12061 ttctttcgaa ttaacgttta ctaaattgtg atgctttacg agctttctta agacctggtt
    12121 tcttacgttc aactttacgt gagtcacgtg taagaagtcc tgcgcgtttc aatgaatcgc
    12181 ggaagtctgg gtctacttga agaagggcac gagcgatacc gtgacggata gctcctgatt
    12241 gaccagcgta tccaccacct acaacgttaa cgaaaacgtc gtatgaacct acagttgaag
    12301 taactgcgaa tggttggttg atgacaagac gaaggtcagc gtgtgggatg tactcttcaa
    12361 catctttttt gttaacagtg attttaccag ttcctggaac aaggcgaacg cgtgcaacag
    12421 cgtttttacg acgtccagta cctgcatatt gtgcttgtga catactttat tgttcctttc
    12481 cttagataag tcctgaaatg tcaagaactt ctggttgttg tgcagcgtga gtgtgctcag
    12541 ctccaacaaa tactttcaac ttcatacctt gagcgcgtcc aagtgtattg tgtggaagca
    12601 tacctttaac tgatttctca atcaaacgta ctgcattttt agaacgaagt tcacctgcag
    12661 agatttgttt caatccacct gggtggtttg agtgagtgta gtagatttta tcagttgctt
    12721 ttttaccagt caatttaact ttttcagcat tgataacaat cacgaagtca cctgtatcag
    12781 tgtgtggtgt aaatgttggt ttgttttttc cgcgaagtac gctagcaact actgcagaaa
    12841 gacgtccaag tggtacatca gttgcgtcaa ctacgtacca tttacgttca acttggcctg
    12901 gtttagccat aaatgttgtt ttgttcatga tttctcctat atatagttcg atttttgttt
    12961 acggtgatgg gtgttccttc ccatcgaaaa gtatttggaa ggttccgggg cctttcaaat
    13021 ggggtaaaca ataccgccta ctatcatacc aaatttcacc cctaaaagtc aatagatatg
    13081 aaaaatattt ttcggtaact tctcaaagta actgaaagtt atataatcaa gttaatttgg
    13141 gaatttacct attaacttcg gtagattttt gggaattttt ttccttcttt ttccctgtag
    13201 ttaaaatcct tttcaaaaaa tcaccttttc ttgattttta atcctatttg ctgtatgata
    13261 agggaaaaga aaggggacag agatatggct tttaccaata cccacatgcg gtctgctagt
    13321 tttggcattg ttaccagctt gcctgatgac atcattgact ctttttggta tattatcgac
    13381 catttcttaa aaaatgtctt tgaattggaa gaagaactcg agtttcaatt gcttaataac
    13441 caaggaaaga ttaccttcca cttttcaagt caacacctcc ctacagccat tgattttgac
    13501 tttaaccatc ctttcgaccc tcgttatccc ccaagagtac tggttttaga catggacggt
    13561 agagaaacta tcctcctccc agaagaaaat gacctatttt aatactcaat gaaaatcaaa
    13621 gagcaaacta ggaagctagc cgcaagctgt acttgagtac ggtaaggcga cgctgacgtg
    13681 gtttgaagag attttcgaag agtataaaaa ctctagcctt cagttgcaag tgactgaaaa
    13741 ctagagtttt tctatttttt caaagcatca tacaagttgc ggatcggttg ttttaatatc
    13801 ggatggataa aatgaggcgc aatttcctgt aaggactcaa ggacaaaaag gcgttccgct
    13861 atgtaaggat gaggcaatat gaggtcgtct gtataaagga tctggtcctc cacaaagagc
    13921 aagtccaaat caatcaaacg aggtccccaa tgcacttctc tcacccgtcc cagctctgac
    13981 tcaatggcta acaaggtttc taacaagtct tgtgctggta gccaggtttc cacctcaacc
    14041 acttgattgg caaagctatc ctgctccact ccaccccaag gctccgtcgc taagacactg
    14101 gactctttga gaatatggat gccacgagct cgcagtttgt caatggcttg cttcaagttt
    14161 gcttgcttat ctcccatatt gcttcctagg gcgataaagg ctcgttgctt gcggcgatga
    14221 atggttaccg agcaagtatc tagtgacaaa tgcaccggtg cccaaggttt tttcagttcc
    14281 aacttcattt cttggacaag aggataagac tcaaaggtac gttccaccag tttataggct
    14341 accgtttcaa tcaaatcttc actcgtttcc tgaaaccaag tcgtccactg ctgacacaat
    14401 tctccgtaat ggacagaggc tgttaaatcc aagtctgtag ctgccttggt catatcatag
    14461 gataggatgg ctgaaacgac aaatttctgc cccaattctt tctcactagg aaaaagacca
    14521 tgataggcaa acatttccaa atccttaatc tgcagttgat ccataatgat tcctttctag
    14581 aaaaatccgc ttgaaacgga ttctttttat agccccatta aacgataagc ttggtcgcgg
    14641 aggtccttat ctgtttcaaa tagaccacga gctactgtcg tcaaggttgc agtgcctggt
    14701 tttctaacac cacgcatact catacacata tgttccgcct caatgacaac aaaggctcct
    14761 ttagcaccta gatagtccat caaggcatcg gccacttcga tattcaaacg ttcttgaatt
    14821 tgtggttttt tcgaataaac ttcaaccgta cgggctagct tagacaagcc tgccacacga
    14881 ccatctggaa tgtaggcaat gtgcgctcta ccataaaatg gcaagaagtg gtgttcacac
    14941 atggtatgga aaaagatatc cttttctacc accatattat cgtcaataat ttcaaaggat
    15001 tttgacaaat gttcctctgc tgtttgacca agacctgaaa aaatctcttg atacatacgg
    15061 gctacacgag caggtgtttc ctgcaagccc tcgcgattag cgttctctcc tacagcctcg
    15121 ataatcattt ttacagctgc ttcaatcttt tgtgtatcca ttctcgttct tcctctccat
    15181 cagataggcc ctcacttggc tcaagaaata caaggaacct gtgacaatcc taactgtttg
    15241 tttctcttct tttttatctg tcaaattctg ctctagaaaa tcatgccaat cttggtagct
    15301 gagatttcta gacttagctg cctctttcag cacgctttca tccgtcgccc gactatccgc
    15361 aaaatgtgtt agagtaagct cggtatctgg catggctccc agcaagtcca acatatcctc
    15421 caaggctttg gttttgatac aagtgaagag gatttcctta tgataatccg caaaacgttc
    15481 ttgcaaggtt accaacaagg ccttgatagc atggggatta tgggctccat ccaaaatcat
    15541 caagggatct cttgacacga tttccaaacg ccctggccaa cttatttctt ccaaggcttg
    15601 accaagaaag tcattgcttg ctagctctcg accatcttct tgacaaaaag tatcaagtaa
    15661 agctatggcc atcccagcat tctctatttg gtacaaacca agcaggctag tctggaagcg
    15721 accttgtctg acagcacttg tatagtcaaa gacttcccct gtcaccacac tttcttgatg
    15781 acgaacctga taatctgtcc cgtaggcaag tctcggcgca tctttccctt ccgcaatgcg
    15841 gtcaatcaca gccaaggctt ctggagcaat acgccctgtt accaagggca tcccttgttt
    15901 gataatacct gccttctgct ctgctatggc ctccaaggtg tcaccaagta gggccacatg
    15961 atccaagcca atagttgtaa ttcctgtcaa aatgggctga cagacattgg tactatccaa
    16021 aagtccaccc atgccaactt ccatgatggc cacatctact tgctctgagg caaagtagtc
    16081 ataggccagg gctgtgataa tctcaaactc ggttgtgccc tgtaaattgg cgaccgcttc
    16141 tccccccatc aaagactgat agtctgccat gagagcttct agcctcgctt ctgagatcga
    16201 ttccccattg atgctaatct ggtctgtgta atgaatgaga tagggcgagc taaacacgcc
    16261 aactctcagc cctagctttt ctagcatctt tttcaaaaaa gcaatagtcg agcccttgcc
    16321 gttagttcct ccgatatgga ggaccttgag tttgagatgg ggattgccac gcaaagctaa
    16381 cagttccacc attcgttcca agccaaaatg cggttgatcc gtccggtagt tagcaatcca
    16441 ctgattgttt tcaatttctt tcatcttatt tatattgttt taaatctaaa ttttccgctt
    16501 catcagccag acgaatagta gaggcaattt caactgccat cctgtgacta gctacgtcat
    16561 gcacgcgcac cacttctaca ccctgtctcg cagcgatact ggttacatga gccgaagccg
    16621 tgtcccgatt gcggaaacca agctctgtct caggattgac ttcaaaacca ttctcctcta
    16681 ggatattgat gacaaatcgc ttgcgcgaaa ctccgagaaa gattggatag cctttctgat
    16741 gtagtttatc caggtcccgt aaaagaagca gattttcttt cttggtcaga ccaaagccaa
    16801 ttcctggatc caacaggata ttttctggtg caataccagc ttctgccgct ctcgctagtg
    16861 ctcgttcaaa gaaagccacc atcaagtctt cgattggcaa tgtttcaaag tcagctaact
    16921 ctttttctgt aaaggtttga ccaaaaccaa aatgagggaa gataagcgaa ctaggatgct
    16981 gaggtcgagc cataactggg ttaaacatga tgaccacttt cgctctcgct tcagctacca
    17041 catgagccat tttctcatca cccataagac cagtgatatc attgactaga tcggcaccag
    17101 cagccaaagc agcctctgct acttgactct tccaagtatc aatagagatg aggacatcac
    17161 tttccttgcg aatcgctttg atcactggaa caacacgctg gatttcctct tctatctcaa
    17221 catagctact tcccggccga gtcgattctc cgccgatatc tagcatactg gctccttctg
    17281 ctatcaattt acgagcctgc tggagcgcct gctcaagagc aaaaaattga ccaccgtccg
    17341 aaaaggagtc tggggttaca ttgataattc cgcaaataac tgtctttgca tgattggctt
    17401 tacttgacat atcggtcact ccctcaagga ttttcatcat attatttttc tattttacca
    17461 taaaaagaaa aagatggaca cgattgcatt catctttccc tagtagaaag aggtcggttc
    17521 ctacataaaa acttttaaac agaaattcct aatgtccgac tcataatcac cacaagagcc
    17581 aacaaacaga aagcaatccc attaacaatc atgtgaagca agatcgacat ttccaaacgt
    17641 tgggtcttgt aggccgtcca agatagaact gtcgacatac ctccataaat caataaagaa
    17701 ggtaaattac ttggttgatg caataaagca aacacaatcg taccgactac aaatcccaag
    17761 ttctccttgc ctcggaaaat ctttttagga acaagcccac ggcacaagat ttcctcacaa
    17821 atcggagcaa gcaaggctag caagaagaaa ctggaaatca acgaactatt ttgaaccata
    17881 tcgttaatct gagactggtt agctgttgtc gtctcatttg acagttgcaa caagatagaa
    17941 ccaagtatat ttgacccgac aataactaaa taactcaagc ccaaacgtgc caaatcttta
    18001 gctctaaaaa aagaaaaatt aaaactagct aacttggttt tacgagctcc cataataaat
    18061 agagccagaa ccacaattga aaggccagca actatcagcc ctgactgtag cagggctact
    18121 tcttttaaag tcaaaataga ggtaaccact aggggaacct gatataaaag gacggcaagt
    18181 aaaaagacca aaatccagcc tgcacgattc aacaattctt tccacatatt tttctctttc
    18241 atcacctatc tcctttgtat taaaaaaggg gggaaatccc cctgggtcat tctattataa
    18301 ggccatgctg atgtagttaa ggataaacaa ggcatccaaa atccaaatca tggcatgaac
    18361 atctttaact tgacctttga caagcttagt caaagtgtaa gtcaagaaac caactgcaat
    18421 cccttgagtg atagagtagc tgaatcccat aaagatagat gtgaagaagg caggaactgc
    18481 ttcagacata tcatcccaat ggatattttt caagctacca agcatcataa tcccaacgat
    18541 aatcaagatt ggagctgtag ccgctgttgg tacgatcgct agaagtgggc taaagaagct
    18601 tgaaatcgca aaacagatag ctacaaccaa ggctgtcaaa ccagtacgtc cacctgcacc
    18661 gataccagca gcagactcaa cataagtcgt tacgtttgaa gtacctgcaa tggcaccgac
    18721 tgtcgttcca atcaaatcag agtaaagagc cttatccaat ttggctgatt ggtgattttc
    18781 accatttgtc gctacgatac caactttttc acctgtaccg atcaaggtac caattgtgtc
    18841 aaaaatatct gtcaatgaga aggcaagaat ggccatcaga gtttcaggca agcgagctgt
    18901 gtctgaaacc aaagctccca aaccttctga accaagagct gcaccaaaga ttgtcttcaa
    18961 atcttcaaaa gctgcaccaa catggttatt agcaaaatcg atactagaca aatcaaccaa
    19021 accaactgca atagcaagaa cagttgttgt caagatagag agaataattc cccctttaat
    19081 ccctttgatg acaaagaaga tagtaatggc aagtcctgca agagccacta aaacagctgg
    19141 attattaaag ctgaccaatc ctggaactgc tgaagagttt gctgcaatcg ttgcttgagc
    19201 tttgtcagcc ccttctccta caacagtata gttgcctgga tcaatcgtaa atttcaaaag
    19261 tccagcattc ttaatcccta cataggcaag gaagacaccg ataccagctg aaatagccga
    19321 gcgaagggca tttggaatcg attcaatgat cattttacga acctttgtca aggtaataac
    19381 caatgaaata atcccacaga tgaagaccat agctagggct tcttgccatg aataaccaag
    19441 tccaaatacg actgtaaagg taaagaaggc attgagtccc atacctggtg cttgagcgta
    19501 tggcaagtta gcatagaagg ccatcatcaa ggtaccagca actgcaccga taatcgttgc
    19561 aagaaacaca ccctgagcag gcattcctgt ttgcgaaaga atttgtgggt ttacaaagag
    19621 aatatagctc attgcaaaga aagttgttaa accagcgaga acctctgtac gaacgtctgt
    19681 accgttctct tttagtttaa ataatttgtc catcatcaat ctccttttaa tttttacgaa
    19741 caataataga attatatcat ttatcattca ctttttcaat gtctttgttt gatttgttta
    19801 agaaattaat ccaatttcat tttttgtttg gaatctgctt ttctgcctgc tttctgttat
    19861 aatagaagac atcttatctg aaaacacact agaaaggtcc atatgacgaa aaaaattatt
    19921 gcagttgacc tggatggaac cctgctcaac tcagacagtc aaatttctga ctttaccaaa
    19981 agaaccatca aaaaagttgc tgaaaaaggc catcaggtta ttattacgac aggtcgccct
    20041 taccgtatgt caaaagattt ttaccgtgaa ctgggcttag acactcctat gattaacttc
    20101 aacggatccc ttactcattt accagaccaa gtttgggatt ttgaaaagtg tttgactgta
    20161 gacaaaaaat atctgctaga tatggttcaa cgttcagagg acattcaagc cgattttatc
    20221 gctggagaat atcgtaaaaa attctacatt acaaatccca atgaagaaat tgccaatccc
    20281 aaactatttg gtgtagaagc tttccagcct gaagatcaat tccagcctga attggtgacc
    20341 aaggacccta actgtatcct cttgcagacc agagccagtg acaaatatgc cttggcaaaa
    20401 gaaatgaacg ccttctacca gcatcaactt tctatcaata cctggggagg tccgctcaat
    20461 atccttgaat gtaccccaaa aggtgtcaac aaggcctttg ctttggacta cttgctcaag
    20521 ataatgaatc gtgacaaaaa agatttgatt gcctttggag atgaacacaa tgataccgaa
    20581 atgctcgctt ttgctgggaa gggttatgcc atgaaaaatg ccaatccaga gctactccct
    20641 tatgcagatg agcaaatttc ccttaccaac gaccaagatg gggttgccaa aaccctacaa
    20701 gacttattct tataacctat actgatactc aatgagaatc aaagagcaaa ctaggaagct
    20761 agccgtaggt tgctcaaagc actgctttga ggttgtagat gaagctgacg tggtttgaag
    20821 agattttcga agagtatgac cagcttgcaa gctggtcttt gtgctctttt cacagtctca
    20881 tcaaccagtt aatcgattgt tctacttttt gtctccaaaa ccttggaaaa ggcttccttt
    20941 tgtgatatac ttcacatata aatacaagag agctcgtcac actcgactct gttgacaaaa
    21001 aatcaatcca gtcccgttgt ccctgttctc ggtcaatatc aaggaggata gctatgaaag
    21061 ctgttgttgt aaatccagaa agcactggtg ttgctattga agaaaaagta ctccgtccac
    21121 ttgaaactgg ggaagcactt gtagaagttg aatactgtgg cgtttgccac accgacctcc
    21181 acgttgctca tggtgacttt ggtcaagtcc caggacgtgt tcttgggcac gaaggtatcg
    21241 gtatcgttaa agagattgct ccagatgtga aaagccttaa agtcggtgac cgcgtcagcg
    21301 ttgcttggtt ctttgaagga tgtggcactt gcgaatactg tacaactggt cgcgaaaccc
    21361 tttgccgtac agtgaaaaat gctggctact cagtagacgg tggtatggct gaacaatgta
    21421 tcgtaactgc tgactatgct gtcaaagttc ctgacggact tgatccagcc caagcttctt
    21481 ctatcacatg tgctggagta acaacctata aagctatcaa agaagcaaaa gttgaaccag
    21541 gccaatgggt tgttctttac ggtgctggtg gacttggtaa cctcgctgtt caatacgcta
    21601 aaaaagtatt caatgctcat gttatcgcag tcgatatcaa caatgacaaa cttacccttg
    21661 caaaagaagt aggcgctgac attgtgatta acggcctcga agttgaagat gtagctggac
    21721 tcattaaaga aaaaactgat ggaggagctc attcagctgt cgtaactgct gtgtctaaag
    21781 ttgccttcaa ccaggctgtt gactccattc gtgctggtgg tcgcgtcgtc gctgttggtc
    21841 ttccttctga aatgatggaa ctcagcatcg ttaaaacagt cctcgatgga atccaagtca
    21901 tcggttctct tgtcggaact cgtaaagact tagaagaagc cttccaattt ggtgcagaag
    21961 gtctggtagt cccagttgtt caaaaacgtc cagtagaaga tgctgttgcc attttcgacg
    22021 aaatggaaaa aggccaaatc caaggacgta tggtactcga cttcacccac taatttaata
    22081 gaaacctatt ttaaaagttg gaagcttctt ccaacttttt tatattaaat tttaaataag
    22141 aattcaagat tactcctcaa aaactcaaca tatcaacata caaaaataaa aattttttaa
    22201 ttttttatga taaatagttg ctttttttat gaaaacggtt tatacttaaa cagtcttaaa
    22261 gttttcttaa atgaaaacta aaacaaaaaa ggaggaatga caataatgag tatcggaatc
    22321 attattgcga gccacggcga atttgctgcg ggtattcatc agtcaggatc tatgatcttt
    22381 ggtgaacaag aaaaggttca agttgtgacc tttatgccaa atgaaggtcc tgatgatcta
    22441 tacgctaagt ttaataacgc tgttgctgca tttgacgcag aagatgaggt tctagttttg
    22501 gctgaccttt ggagtggttc tccatttaac caagctagtc gcgtgatggg agaaaatcct
    22561 gagcgtaagt ttgccatcat cacaggactt aacttaccga tgttgattca agcctacaca
    22621 gagcgcctca tggacgctgc tgcaggtgta gaaaaagtcg ctgctaatat cattaaagaa
    22681 gccaaagatg gcatcaaagc tcttccagaa gagctaaatc cagttgaaga agttgcaagc
    22741 gctgcagctg ctccagttgc ccaaactgct atcccagaag gaactgttat cggagacggt
    22801 aaattgaaaa tcaatcttgc ccgtcttgac acacgtctac ttcacggtca ggttgcaact
    22861 gcttggactc cagattcaaa agcaaatcgt atcatcgttg cttcagataa cgtggctaaa
    22921 gacgaccttc gtaaagaatt gattaaacaa gcagctccag gtaatgtcaa ggctaacgtg
    22981 gttccaattc aaaaactgat tgagatttca aaagacccac gttttggaga aacacatgcc
    23041 cttatcttgt ttgaaacacc tcaagatgcc cttcgtgcca tcgaaggcgg cgtgccaatc
    23101 aagactctta acgttggttc tatggctcac tcaacaggta aaacattggt caataccgtt
    23161 ttgtctatgg acaaagaaga tgttgctaca tttgaaaaaa tgcgtgactt gggtgttgaa
    23221 tttgatgtcc gtaaagtacc aaatgattct aaaaaagatt tgtttgactt gattaacaaa
    23281 gccaatgtca aataagccat tatttatgaa aggattttaa acatgtctat tatttctatg
    23341 gttttagtag tcgttgtagc cttctttgca ggtcttgaag gcatcctcga ccagttccaa
    23401 tttcaccaac cacttgtagc ctgtaccctt attgggcttg taacaggtca cttggaagca
    23461 gggattatcc tcggtggatc gcttcaaatg attgcccttg gttggtcaaa tatcggtgct
    23521 gctatcgctc ctgatgctgc acttgcttct gtcgctgctg ccattatcat ggttcttggt
    23581 ggtgacttta ccaagactgg tatcggtgtt gcccaagcgg ttgctatccc tcttgccgta
    23641 gctggtctat tcttaactat gattgttcgt acaatttcag ttggattggt tcacactgct
    23701 gatgctgccg ctaaaaaagg tgacttcggc gctgtggagc gtgctcattt cattgcgcta
    23761 cttttccaag gacttcgtat cgcgcttcct gcagctcttc tccttatggt accaactgaa
    23821 actgtacaaa gtatccttag tgccatgcca gactggctca aagatggtat ggctatcggt
    23881 ggtggtatgg tcgttgccgt tggttacgcc atggttatca acatgatggc aactcgtgaa
    23941 gtatggccat tcttcgctct tggtttcgtt cttgctgctg tgtcagatat tactctaatc
    24001 ggattcggtg ctatcggtgt tgctatcgct cttatctacc ttcacctttc taaaactggt
    24061 ggaaatggtg gcggaggagc cgcaacttct aacgacccaa tcggcgatat cctagaagac
    24121 tactaagata agaaaggact gaaaacatca tgactgaaaa acttcaatta actaaatcag
    24181 atcgtaaaaa agtttggtgg cgttcaacct tcttacaagg gtcttggaac tttgaacgga
    24241 tgcaaaactt gggctgggct tatacactca ttccagctat caaaaaactc tatactaaaa
    24301 aagaagatca aatcgctgct cttgagcgtc accttgagtt cttcaacact catccatacg
    24361 tagctgctcc agtcatgggg gttactcttg ctcttgaaga agaacgtgct aacggtgtgg
    24421 aaatcgatga cgctgctatc caaggggtta aaatcggtat gatgggacct cttgctggta
    24481 tcggtgaccc agtattctgg tttacagtac gcccaatcct tggatctctc ggtgcttcac
    24541 ttgcccttac tggcaatatc ttggggccac tcctcttctt tgttgcatgg aacttgattc
    24601 gtatgtcatt cttgtggtat gttcaagaga ttggatacaa ggctggatca gaaatcacta
    24661 aagatatgtc tggtggtatc cttcaagata tcactaaagg agcttctatc cttgggatgt
    24721 tcattcttgc tgtccttgtt caacgctggg taaatattaa atttgctttc gatgtttcta
    24781 aagttcaact agatgaaaag gcttatatcc attgggataa attgccagaa gggtctaaag
    24841 gtatccaaga agcattcgca caagtaggac aaggattgtc tcaaactcct gaaaaagtta
    24901 ctactttcca acaaaacttg gatatgttga ttcctggatt atcaggacta ctccttactt
    24961 tactttgcat gtacttactt aagaaaaaag tatctccaat cactattatc cttgccctct
    25021 tcgcagtggg tattgtggca catgttcttc acatcatgta atcaagcaac taaaaaggaa
    25081 ccaggttcta aaatctgatt ccttttttct atgcttttat tcagccaagg ctcccattgg
    25141 atcccatggt gcaagtacga ttggttctgc tccataggca gcttgttctt ctgctgtcag
    25201 caattcctta cgaacaacga tttggtatgt gtattcgtcc atccaagcgt ctgaggcaac
    25261 aaagtaacca tctgtaccga ccttgtctcc ccatgagttt tcaaccttcc acttggttga
    25321 tttaccattt tcgtccaagt caacacctgt caagaccatg gcgtgggtca tcaagctttc
    25381 gctatagtcc aaacgtccag ccttgtcttg agtaagttta atgtccatgc ttgattcaaa
    25441 gtcataaaca tctgtcgcaa ggatgccagc cttacggttg ctgagctggc cgacatcaga
    25501 accaaaccaa acagtctcac ctgcttgcat ttgggcaatc gccaattctt tcaagcgctc
    25561 cattggaacg ttgatgtagc gaactgcacg gctaccaacc acattcccca acatctcaac
    25621 tgtgtaagat tttccgtaag gtttatcagc agttggagca ttgataacag aaacgtagtc
    25681 ttctaaagga agattgacat atttcttgta aaactcttgt ggtgtgattc ctttttcact
    25741 tttatagttg ttatctttat cgcgataagc aaagtcaaac ttgcgtggtg gaagtcctaa
    25801 tgacatagca agaaagttaa agatttcttg caagaggtct tctttcttag cttgaacagt
    25861 cgcttgatct gcaccagaaa caagcaaatc acgcaagatt tgagcatctt gacgaagcaa
    25921 tttattaagg atcgcattta gctcacgact gctgctagat gaaacagact caggataaac
    25981 tgacttaggc acgacaccgt atttttcaaa gagggaaacg accatatccc attgaccgcc
    26041 atcttgttga ggtgtttgga gtaagaagct aaccttgcgg ctagtcaatt cttggtctga
    26101 agtcgcaatg acttgctcca agaaccagtt tgatttctca tacttatccc agaagaaagt
    26161 gtgggcttgt gacaactcaa agttctccaa tttgtattgc gagatgagtt tgtggcggaa
    26221 ggtgttgaga gccgcaaaca tccagcaacg accagacgct ttctggttag tgaccttgtc
    26281 cttggttaaa tccaatgaga aaacaggtgt gttgtctaca tggctttggc gacattccag
    26341 agctgcaaaa attccgttgt ggctggcagc attttcaatc gcttggtatt ttacatttgc
    26401 ttcatagttg gcaaatagtt tatcagtaaa tgattcttga atcgcgttca tagattcctc
    26461 cttttattct acagtctatt ataactcttt tcacaaagga agcaactaaa aaacataaaa
    26521 ggcgaggaca ttgtccctca cctttttcga atttatactc ttcgaaaatc tcttcaaacc
    26581 acgtcaacgt cgccttgccg tatatatgtt actgacttcg tcagttctat ctgcaacctc
    26641 aaaacagtgt ttttagctga cttcgtcagt tctatctgca acctcaaagc agtgctttga
    26701 gcaacctgcg gctagtttcc tagtttgctc tttgattttc attgagtatc agatttagga
    26761 aattaacttc ctcgtctcca aaaaatagct aaaacaatca tggctcctaa aatcgctggg
    26821 ataatagctg ttcctgataa aactggcccc caagttccaa agagcaagtg acctagaaag
    26881 gctccgatcc aaccgagaaa catttttcca aaacatccca ttcgctctcc acgattggtc
    26941 atagcacctg ctaaaaatcc cactaggaga ccaacgaaca tacttcctaa catattatct
    27001 ccttaatttg cccaattccc attacggaaa agaggtaccc gcgttccatc ctcacgaata
    27061 ccatcgatat ccatttggtt agaaccaatc ataaagtcta cgtgaacatc tgaacggtta
    27121 agccctgcag cttcaagctc ctcttcgctc atctccgctc catcaacaac gctagtcgca
    27181 taggctgcac cgatagccaa gtggtttgac gcattttcat cgaaaagggt gttaaaaaag
    27241 gtaatgcctg actgagaaat tggacttgga tctggtacca aggcacattc acccaaggca
    27301 cgcgcacccg catttttaaa gacaaggtct ttcataacct gatcaccctt ctcagcagtg
    27361 atatctacga tttgtccatc cttaaaggtc accttaatgc cttcaatgat atttccgttg
    27421 tagctaagcg gttttgtaga agtgacataa ccatctgcac gacggaagtc aggcgctgtg
    27481 aagacctctt ctgtcggcat atttggcaag aattcttcgc cctgtgcatt gacagcacca
    27541 gctgattccc aaacgtggtt ctttggcaaa ccaagtgtta aatctgttcc tggcgctgtg
    27601 tagtgaaggg ctgaaaattg ctccttatta agcatatcgg ccttgctctt gagaatggct
    27661 gcatgttcct cccaagcctt aacaggatct gcttcgtaga cacgacaagt tttgaagatt
    27721 tgatcccaaa ggaaatcaac tgcttcttcg tcgctcgcag catttgggaa gactttctta
    27781 gcccactcaa gtccagcagc ggctgctaca gtccagctaa ccttgttaga ttgagttgcg
    27841 atacgcatag gcttcatggc aagtcccata gctttagcag aagctgaaag cttgtcagcg
    27901 tccactccgt tcaaggcacc tggatcagat gaacgaaccc caagacggct agccttgttc
    27961 tccaaaagat agttcatctc agcaatcttg tattctggca cattgtccaa acgctccatc
    28021 ggggcatgga ggaatttctc acggttaatc acatcatctg tccactgaac gatgacctca
    28081 tgcgcaccca aggcataagc ttctttcacg attagatgtg ccaattcacg ttgctccaca
    28141 tcaatagaga gagccaaagt gtgaccaggt tgcacgttaa ttccgttcgc aaccaacaat
    28201 ttcgcatatt ttcctagatt ttctttaaaa tttggtaaaa ccattctgtt ttctcttttc
    28261 tatttttatt tttctttcaa agcagaaaag attcctgcta aagcaatagc acctgacaag
    28321 agtgctgtcg atagaagaat tgtacgatca gcaagttcta attgatattc aaataccttc
    28381 ttagccggct tatcttccgc aaatattctt agtttcatga gaaccccctt aaaatagatt
    28441 aaaataaatc actgtgactg cctgtcctca gcaaatttaa aatcaattcc gacttgtcta
    28501 ctttataaac caaaagccaa tctggctggg tatggcattc acgaactcct tgaaaatgct
    28561 tggatgccgt caatgaatga tcacgatatc tggcaggatg ttctttttct tgaaccagaa
    28621 aattcaaaac ttcttctaat aattctgcct tcaaaccacg cttcatagcc aacttaaaat
    28681 cttttttaaa ctgtttatga taacgaatct taagcacgta agtcctccat caaatctgag
    28741 actgattcaa aagactggct catattacga ttttggtcta aatccgttaa cgcttggagc
    28801 aacttccgat tttcgtctag tctaacatca aaaggtaatc cctgatattg aattgcctga
    28861 cgaaggaaaa tattaatagc tgttgtcata tccattccca gattactaaa cacctgttgg
    28921 gcctgctcct taacctcact atccagacgg atgcttatac tcatctttga catactctca
    28981 ccctctttcc atagactatt ttaacaaaaa agaaagctaa tgtaaatcta ttggatatac
    29041 gttagcctct tttaatagat tattaagcaa ttttttaaaa caactcatca aacaaactca
    29101 actggttatc ctctggcata tttccaagaa tacccatctc atccatcttt tcaaccaagg
    29161 ttgatgagag tccaccacgc ttgcgtagtt ctgttttaga gaggaattct ccctcttcac
    29221 gcgcccgcac cagttgcttg gcaacgttct ctcccagacc atccattgct acaaatggtg
    29281 ggataagggt atccccgtcg atgaggaact ctgtcgcctg actacggtag agatctaatt
    29341 taccaaactt gaaacctcgt tcccacatct cattgacaat ctcaagagtt gtatagagat
    29401 cgatttccac attagaggct tcattgttct tccgtttttc agagatttct tccattctgc
    29461 gcttgatgac ctccaagccc gcacccatgg tcttgatatc aaaagcctta gcacgaatgg
    29521 agaagtaagc acagtagtaa taaataggat ggtgaacctt gaagtaagct acacgcaagg
    29581 ccatcataac gtaggctgcc gcatgggcct tagggaacat gtacttaatt ttcccacagg
    29641 attcgatata ccactctggc accttattag ccttcatggc ttcgatatag ccatttctct
    29701 cctcttctga aatctttagc cacaaaccct tacgtacccg ttccataatg gtaaaggcca
    29761 tcttaggttc cagacccgca tgcatgaggt aaaccatgat gtcgtcccga caaccgataa
    29821 cagtcgatag gtccgctatt ccttgcttaa tcagatcctg agcattcccc aaccaaacgt
    29881 cagtaccgtg ggacagacca gacagctgaa gcaattccgc aaaggttgtc ggatgggttt
    29941 cgtctaccat tccacgtacg aaatttgttc caaactctgg aatccccaac atacccgtag
    30001 gcgttccaat ttgttcaggt gttaccccta gtacatcagt cccagaaaag agtgccatca
    30061 cgccttcgtc atccatagga attttattag ggtcaatacc agacaaatcc tgaagttttc
    30121 gaatcatggt cggatcatca tgtcccagta catcgagttt gaggacgttc tcatcgatat
    30181 cgtggaagtt aaagtgagtg gtctgccatt cagccgtgac atcatctgct ggatactgga
    30241 caggcgtaaa atcgtagaca tccatgtagt tcggaataac aacgattccc cccgggtgtt
    30301 ggcctgttgt ccgcttgaca cccgccgctc cttgagcgag gcgttctact tctgcatcac
    30361 gataaaactt gccataatct cgctcgtaac ccttgacaaa tccataggca gtcttggcag
    30421 ctaccgtacc aaccgttccc gcacggaagg catattcttc accaaagata tcacgcacat
    30481 ccaagtgggc gctaggctga tcttctcccg agaagttcaa gtcaatatca ggaaccttat
    30541 ccccatcaaa accaaggaag gtctcaaacg gaatatcctg tccgttttta ctgagtttgt
    30601 gaccacagtt tggacagtcc ttatggggca tatcaaatcc tgaaccgtac gaaccatctg
    30661 tgataaactc actgtactga cactgaccac agacatagtg aggagagaga ggattgacct
    30721 ccgtaatccc aatcatggtc gcaacgaaac tagatccgac agacccacga gaaccaacca
    30781 aataaccccg ttcattagaa cgttgcacca gcatctgcga tgccagataa atcacagcaa
    30841 atccattccc cagtatggat gttaattctt tttcaatccg caaatcaaca atatctggca
    30901 gcggatttcc ataaatctca aaagctttct tataggtcaa ctcagcaact gtttcttcag
    30961 ccttgtcgat gaaaggcgta tacaagtcac ccttaacgac ttcaacggat tcaaatattt
    31021 ctgccaaggc attggtgttt tcaataacca gtttacgagc cagttcctct cccaaaaagg
    31081 caaattcatc caacatctca ttagtcgttc gaaaatgagc ctttggaagt ggtgctggtt
    31141 gggcatgttc accatgaccg atagttcgat taatcatcgc accctgtccc aaactacgga
    31201 cgataatttc acgataaatc tcttcttccg gttcgatata gtgaacattt cccgtagcca
    31261 gaacaggctt gccaaggcgg tctccaacct ctatcaaact cttgataatg gtctggagtt
    31321 cctccatatc cttgacctgc tctttggcaa tcaagggtgc atagatagcc ggtggcatga
    31381 cctcgataaa atcataatac ttggccacct caaccgccgc atccacacct tgagaaacga
    31441 ccacgtcaaa aacttcaccc tctgaacagg ctgaacctaa aatcaagccc tctcgatggg
    31501 catctagaac cgttctcgga atccgtggca ctccttcaaa atacttggta ttagacaagg
    31561 aaaccagctt aaagatattt tttagaccta cctgattctt gacatagatg gtcgcatgct
    31621 tgatccgagc ttttttgtaa gaatctggac tgattagatc aatgttgagt ctagctaaat
    31681 cggtcacacc atgtttttct gctacctctt tgataaagat gaaaagcaga cgaccagtcg
    31741 cttccgcatc gtagttggcc atgtggtgat gttccaaggc cacaccaaaa cgcttggtca
    31801 aaggccccaa accatggcgt ttatactcag gatagaggtt tctagcaaac tccagcgtat
    31861 caataactgg ctgactaatc tttggaagat catgccgctc ataattagca ttcataaagc
    31921 caacgtcaaa ggtagcattg tgggcaacta ggaccgtatc cttgcaaaat tcttggaatt
    31981 cttgcaaaac ttgttctagt ggtttggcat ttttgacatg atcatctgta attccagtta
    32041 actctgtagt aaaggctgac aagggatgcc caggattgat aaattcatca aattcagcaa
    32101 taacattccc cttgtacatc ttagaagccg caacctgaat caagtcatta tagatagctg
    32161 aaagtcccgt cgtttccacg tcaaagacca cgtaggttgc ttctgacaag tccatctcca
    32221 cttcgttata gacgataggg acacggtcct ccacgatatt ggcttccatc ccatagatca
    32281 gctggattcc cgctttctta gccgccttat agccatgtgg aaaggactgg acattcccat
    32341 ggtccgtgat agcaaccgcc ttgtgtcccc acttagcagc tgttgcaaca atctcttcga
    32401 cctctggcaa agcatccata gtcgacatgt tagtatgagc atgaaactca acccgacgct
    32461 caccttctgg catcaaatcc ttccgctcat agtgaacaac ttcctgcaga tcctgtacgt
    32521 tcatagtcaa atcgcgtgtg aagttattca tctccacatt ccctcgaact cggagccaag
    32581 aattcttctt gatgaggtca aacttctggg cctcttcctc gtttttaacc cacttttgca
    32641 tagaaaaact tgaagtatag tccgtcattt taaagttgat taaaacacga cctgttctag
    32701 tcactttttg ctccacatca aaaacaaccc cttcaaatac cagacgattt tcctctgtcg
    32761 tcacttcgat cataggagta atctccgcct tatccagctt gggtttagct gcagcttttt
    32821 tcgcttgaaa atcaaaggct ggtttctctt ccgctggagg aggtgccatc tgctccagtt
    32881 gttccatagc acggagcgct tcctcattgg cagcttgaac aatctgctca ttttcagcat
    32941 gaaaggcctc ttcctgctct tgggtcagga catcattctt ctcgacttga cagttaaaag
    33001 ttggaaaacc aaacttttca agttgtttgg ctaaattagg aagatgattc ttcttaaaat
    33061 gttccttatc aatcgcttca gatccttcaa taaatagctg attaccctca gcacgaactt
    33121 gcaaattttg ataaagggac ttaaaacctt gactagcaca tggaccttca gagaaagcct
    33181 ccctatagta ggactgcaag agctgatttg aaaattcttg agaccgagcc ttaatttcaa
    33241 aaacagcttt attgcctgtc ttagaaaatt cttcgctcaa acctttcttt aattctaaaa
    33301 agatttcaat cggtaaaata ttagaaaata cgaaatgaaa ctcccatacc ttactaattt
    33361 tatgaaccac aactcgctca atattggcct gtgctaaagc aggagcctgt ctcatttcag
    33421 caggcatccc caattgattc atcaaaattt caaaactatt tgacattcat tttcctcaca
    33481 ttattcttct actattttac catatttaga ggtattttct aaagacaaaa ggaagccact
    33541 aagtgacttc cttctagagt gaggacggat tagtcttcac ctttattttt cttaataatt
    33601 tcttcttgta ctgacttagg tacatcttcg tagtggtcaa ataccatcat gaatgtacca
    33661 cgtccttgag atgcagaacg aagaactgtt gcgtaaccga acatttcagc aagtggaacg
    33721 taagcacgaa cgatttggct gttaccgtgt gcttccatac catctacacg tccacgacga
    33781 gcagttacgt gacccataac atcaccaagg ttttcttctg gaacagtgat tgttacaagc
    33841 atcattggtt caaggatagc tggttgtgct gatttagcag cttctttaag ggaaagtgaa
    33901 gccgcaatct tgaaggcagt ttcagatgag tcgacatcgt gatatgaacc atcataaagc
    33961 ttagctttaa cgtcaaccat tgggtaacct gcaagaacac cgttagccat agattctacc
    34021 aaaccttttt caaccgctgg gataaattca cgaggaacca caccaccgac gattgcgttt
    34081 tcgaattcga atcctttacc ttcttcgttt ggagtaaatt caatccatac atcaccgaat
    34141 tgacctttac caccagactg acgtttgaag aatccacgtg cttgagtaga agcgcggaat
    34201 gtttcacggt aagatacttg aggagcacct acgttcgctt caactttgaa ctcacgacgc
    34261 atacgatcaa caaggacgtc aaggtgaagt tcacccatac ctgagataac tgtttcacca
    34321 gtttcaacgt ttgtttcaac gcggaatgtt ggatcttctt cagccaattt ttgaagggcg
    34381 atacccatct tgtcttggtc agctttagat tttggctcaa ccatcaattg gataactggt
    34441 tctggaacgt tgattgactc aaggatgatt ttagcttttt catctgtcaa tgagtcacca
    34501 gttgtagtat ctttcaaacc aacggcagca gcgatatcac ctgagtaaac agtgtcgatt
    34561 tcttgacggc tgttagcgtg catttgaagg atacgtccga tacgttcacg tttaccttta
    34621 gaagtattca atacgtatga acctgattga agaacacctg agtaaacacg gaagaatgtc
    34681 aaacgaccta cgaatgggtc agtcatgatc ttgaaggcaa gagctgcaaa tggctcttcg
    34741 tcagatgctg gacgaatttc ttcagcgtct gtatctgggt taataccttt gattgctggg
    34801 atgtcaagtg ggcttggaag gtagtcgata accgcatcaa gcatcaattg aacacctttg
    34861 tttttgaagg ctgaaccaca caatactggg aagaattcaa cgttgatagt cgctttacgg
    34921 ataccagctt tcaattcttc gttagtgatt tcttcacctt cgaggtattt catcatcaat
    34981 tcttcgtcag tttcagcaac tgcttcaatc aatttttcac ggtattcttg agcttggtca
    35041 aggtattcag ctgggatgtc ttcttcaagg atatccgtac caaggtcgtt agtatagatt
    35101 tcagctttca tcttgatcaa gtcaatgata ccacggaagt catcttcaga accgattggc
    35161 aattggattg ggtgtgcatt tgcttgaaga cgatcgtgaa gtgtgcttac agagtaaagg
    35221 aagtcagcac cgattttgtc cattttgttg gcaaatacga tacgtggaac tccgtactca
    35281 gttgcttgac gccaaactgt ttcagtttga ggctcaacac ctgattgtga gtcaagaacg
    35341 gtaaccgcac catccaatac acgaagagaa cgttgtactt cgattgtgaa gtccacgtgt
    35401 cctggtgtgt cgatgatgtt tacgcggtgg ttgttccatt gagctgtcgt cgcagcagat
    35461 gtgatcgtga taccacgttc ttgctcttgc tccatccagt ccatttgtga cgcaccttcg
    35521 tgagtttcac cgattttgtg gattttacca gtgtagtaaa gaatacgctc agtagttgtt
    35581 gttttaccgg catcgacgtg agccatgata ccgatattac gagttttttc aagtgaaaat
    35641 tcgcgtgcca tgaggtttgt ttctcctatt tatttttgat ttctattcta ttataacacg
    35701 attttaataa aaacggatag gcaggaccta cccgttctca atgttttcat gctattgttg
    35761 gtttcaactt acgagatggt aagctgagtt atagctaata ctaatcgatt tagctaattt
    35821 gaacccgggc taaagttagt tagccgatgt gagctggaac gggatgctgc gtgaaaaaga
    35881 taaaactcct tgtattcgtc gaatactgcg tcagtttcct atttttacct tgcatcctta
    35941 ccgttcctag catcatgatt tagttgagct caagctaaaa gtctggagaa aaagatgaat
    36001 ctcattggtt gcaaccgcaa cctgcttcga ttccctattt tctctgggac tttcttaacg
    36061 ctttcgcatc ctatcttacc aacggaagtg tgcgaatgca cggttagctt cagccatacg
    36121 gtgagtatct tcacgtttct taactgctgc accagtgttg ttagcagcat ccaagatttc
    36181 ttttgcaaga cggtcttgca ttgtgtgttc accacgaaga cgagcgattg ttaccaacca
    36241 acgaagtcca agtgttgtac gacgttctgg acgaacttca actgggactt ggtagttaga
    36301 accaccaaca cgacgtgcac gtacttcaag tacaggcatg atgttttcca tagctgtttc
    36361 aaatacttca agtgcatcgt tgccagtagc ttctttgatt tgctcaaaag caccgtaaac
    36421 gattgaagca gcagtaccac gtttaccatc aagcataacg cggttgataa gacgagtaac
    36481 tagttgtgaa ttgtatagcg gatctggcaa tacgtcacgt tttggagctc tatttttacg
    36541 actcatttct ctttatcccc tttccttatg cttttggacg tttagtaccg tatttagaac
    36601 ggccttgttt acgatcgtta acacctgcag tatcaagtgc accacggacg atatggtaac
    36661 gtacccctgg aaggtctttt acacgtccac cgcgaagaag caccacgctg tgctcttgca
    36721 agttgtgtcc gatacctggg atgtaggcag taacttcgat aaggttgctc aaacgtacac
    36781 gagcgaattt acgaagggct gagttaggtt ttttaggtgt cattgttcca acacgagttg
    36841 caacaccacg tttttgtggt gaagaaacgt ttgtttgaac ttttttatga ctattgtaac
    36901 caacgttcaa agctggtgat ttagattttt ctacttttga tttacgcggt ttgcgaacca
    36961 attggttaat tgtaggcatc tacattctcc tgtgtttttt tatttttggt gatgatacac
    37021 ttggtgacag atatcatctg tgtgtacttt tgcaacattt gtcagcacgt ccctgtacac
    37081 tcttgagaga ccaaaagtaa aaagtaccgt ctattattgt aacaaaattt tcccttgctt
    37141 gtcaagatat ttttcttttt tattggtaat gttagagatc aatttctgaa taaaaatagc
    37201 tgagaacaca tcatcttcct aataacacgg cttggcttac gtgacatttt tatctccgtc
    37261 atttatactg atatataaaa tagtacggaa aacttctcaa aagtaagaat ttctttcaca
    37321 aatcaaagaa aacgcttgca tattgtttta ctacatgcta tactgatata gaaatttatt
    37381 gattggagaa tcattatgag aaaaacacca tctcacactg agaaaaaaat ggtctacagc
    37441 atccgttccc tcaaaaatgg aactggttct gtccttatcg gcgcaagcct tgtcttgctt
    37501 gccatggcta caccaactat ctcatccgac gaaagtacac caaccactaa cgaacccaac
    37561 aacagaaata caaccaccct tgcccaacct cttactgata cagcagctgg ctctggtaag
    37621 aacgaaagtg atatttcttc acctggaaat gcaaacgctt ccctagagaa aacagaagaa
    37681 aaacctgctg caagcccagc cgatccagca ccacaaactg gacaagatcg ttcaagtgag
    37741 ccaactactt ctactagtcc agtaacaact gaaactaagg cagaagagcc catcgaagat
    37801 aactacttcc gtatccatgt caaaaaactt cctgaagaaa acaaggatgc tcaaggacta
    37861 tggacttggg acgatgttga aaaaccatct gaaaactggc caaacggagc tttgtccttc
    37921 aaggatgcca agaaagatga ctacggctat tacctagatg tcaaattaaa gggagaacaa
    37981 gccaagaaaa ttagcttcct catcaacaat acagctggaa aaaatctaac cggcgataaa
    38041 tctgtagaaa aactagttcc aaaaatgaac gaagcttggt tagaccaaga ttacaaggtt
    38101 ttctcttacg agccacagcc tgcaggaact gttcgcgtca actactaccg cacagatggc
    38161 aactatgaca agaaatctct ctggtactgg ggagatgtga aaaatccaag tagcgctcaa
    38221 tggcctgacg gaacagactt tacggctaca ggcaaatatg gccgctatat cgacattcct
    38281 cttaatgaag ccgcaagaga atttggattt ttattactag atgagagcaa acaaggagac
    38341 gacgtgaaaa tccgtaaaga aaattataag ttcacagatt tgaaaaatca tagccaaatt
    38401 ttcctaaaag acgatgatga atcgatttac acaaatccat actatgtcca tgatatccgt
    38461 atgacaggag cccaacacgt aggcacttct agcattgaaa gtagcttttc aacacttgtc
    38521 ggtgctaaaa aagaagatat cctcaaacac tccaacatca ctaatcacct aggaaacaag
    38581 gtaactatta ccgatgttgc aatcgatgaa gctggtaaga aagtgaccta cagcggagat
    38641 ttctctgaca caaaacatcc ttatactgtt agctacaatt ccgaccaatt cactaccaaa
    38701 acaagctggc gcctgaaaga tgagacatac agctatgatg gcaaactggg agctgaccta
    38761 aaagaagaag gaaaacaagt tgatttgacc ctttggtcac caagtgctga taaggtttct
    38821 gttgttgtct acgacaagaa tgaccctgac aaagtagttg gaactgtcgc tcttgaaaaa
    38881 ggggaaagag gaacttggaa acaaactcta gacagcacaa acaaactcgg aatcacagat
    38941 ttcactggct actattatca ataccaaatc gagcgtcaag gtaaaactgt tcttgcactc
    39001 gatccttacg ctaaatctct tgctgcttgg aatagcgacg attccaagat tgacgatgcc
    39061 cataaagtgg ctaaagccgc ctttgtagat ccagctaaac tcggacctca agacttgact
    39121 tatggtaaga ttcacaattt caagactcgt gaagacgccg ttatctacga agctcatgtg
    39181 cgtgatttca cttcagatcc tgccattgca aaagacttga ccaaaccatt tgggactttt
    39241 gaagccttca ttgaaaaact agactatctc aaagacttgg gtgtaaccca tatccagctc
    39301 cttccagtct tgtcttacta ctttgtcaat gaattgaaaa accatgaacg cttgtctgac
    39361 tacgcttcaa gcaacagcaa ctacaactgg ggatatgacc ctcaaaacta cttctccttg
    39421 actggtatgt actcaagcga tcctaagaat ccagaaaaac gaatcgcaga atttaaaaac
    39481 ctcatcaacg aaatccacaa acgtggtatg ggagctatcc tagatgtcgt ttataaccac
    39541 acagccaaag tcgatctctt tgaagatttg gaaccaaact actaccactt tatggatgcc
    39601 gatggcacac ctcgaactag ctttggtggt ggacgcttgg ggacaaccca ccatatgacc
    39661 aaacggctcc taatcgactc tatcaaatac ctagttgata cctacaaagt ggatggcttc
    39721 cgtttcgata tgatgggaga ccatgacgcc gcttctatcg aagaagctta caaggctgca
    39781 cgcgccctca atccaaacct catcatgctt ggtgaaggtt ggagaaccta tgccggtgat
    39841 gaaaacatgc ctactaaagc tgctgaccaa gattggatga aacataccga tactgtcgct
    39901 gtcttttcag atgacatccg taacaacctc aaatctggtt atccaaacga aggtcaacct
    39961 gcctttatca caggtggcaa gcgtgatgtc aacaccatct ttaaaaatct cattgctcaa
    40021 ccaactaact ttgaagctga cagccctgga gatgtcatcc aatacatcgc agcccatgat
    40081 aacttgaccc tctttgacat cattgcccag tctatcaaaa aagacccaag caaggctgag
    40141 aactatgctg aaatccaccg tcgtttacga cttggaaatc tcatggtctt gacagctcaa
    40201 ggaactccat ttatccactc cggtcaggaa tatggacgta ctaaacaatt ccgtgaccca
    40261 gcctacaaga ctccagtagc agaggataag gttccaaaca aatctcactt gttgcgtgat
    40321 aaggacggca acccatttga ctatccttac ttcatccatg actcttacga ttctagtgat
    40381 gcagtcaaca agtttgactg gactaaggct acagatggta aagcttatcc tgaaaatgtc
    40441 aagagccgtg actatatgaa aggtttgatt gcccttcgtc aatctacaga tgccttccga
    40501 cttaagagtc ttcaagatat caaagaccgt gtccacctca tcactgtccc aggccaaaat
    40561 ggtgtggaaa aagaggatgt agtgattggc tatcaaatca ctgctccaaa cggcgatatc
    40621 tacgcagtct ttgtcaatgc ggatgaaaaa gctcgcgaat ttaatttggg aactgccttt
    40681 gcacatctaa gaaatgcgga agttttggca gatgaaaacc aagcaggatc agtcggaatt
    40741 gccaacccga aaggacttga atggactgaa aaaggcttga aattgaatgc ccttacagct
    40801 actgttcttc gagtctctca aaatggaact agccatgagt caactgcaga agagaaacca
    40861 gactcaaccc cttccaagcc tgaacatcaa aatgaagctt ctcaccctga acatcaagat
    40921 ccagctccag aagctagacc tgattctact aaaccagata ccaaagtagc tgatgcggaa
    40981 aataaaccta gccaagctac agctgattca caagctgaac aaccagcaca agaagcacaa
    41041 gcatcatctg taaaagaagc ggttcgaaac gaatcggtag aaaactctag caagaaaaat
    41101 atacctgcaa ccccagatag acaagctgaa cttccaaata caggaattaa aaacgaaaac
    41161 aaactcctat ttgcaggaat cagcctcctt gcgctccttg gtctcggttt cttactaaaa
    41221 aataaaaaag agaactaaac tagccctcct atagaaaaat cccccaagca ttatagctcg
    41281 ggggattaat tttcgtacaa tatttgttgt cctaataaac ttgattagga ttttttatta
    41341 agcctctttc atagcaaaat aagctcgtac tttgggtgca acttgtgttc cgaagagttc
    41401 aatagctctc agaacctggt catgaggcat agaaccaagc ggtagatgaa gcatgaagcg
    41461 gtccaatcct aaatcctcta tcatgcgaat caatttttcg gccacctgat ctggattgcc
    41521 aacaaacatg gcgccatttg accctacctg ctccaaatat tgctcataac gcaattcctg
    41581 ccagtgcgga cggtctttgg aaatagcatc caccacttgc ttagtcggat ggaaataatc
    41641 tttcaccgcc tgctcaccat cttccgcaat ccacccccaa gaatgggctc ccactttcaa
    41701 gtctttgtca gcatggaccg cttcgcttcc aatctcacga taagcctgaa tcaacttttt
    41761 aaaataacgt ggattaccac caataatagc atatacaatc ggtagaccag cctgagcaat
    41821 cttcactgtt gattcgacat gaccacctgt agctatccac aagggcaatt tgtcctgaac
    41881 tggacgagga taaacttctt taccagcaat cgtttgagtc aatcgacctt gccagtctaa
    41941 cttggtcttt tcattgacta actgaagcaa gtctaatttc tcatcaaaaa gagagtcgta
    42001 gtctttcaag tcataaccaa acagagggaa agattccgtg aaagagcccc ttccagccat
    42061 aatctccgct cgtccatttg acaaagcatc gatagtggca tactgttgga acaaacgaat
    42121 cggatccatg cttgacagaa tgctgactgc actggtcaaa cggattttct tggtattgac
    42181 tgccccagcg gccaggacaa tctctggggc tgatactgca aaatccgccc gatggtgctc
    42241 gccaatccca tatacgtcca aaccaacctt gtcagccagc tcaatctctg ccaccaactg
    42301 gcgaatgcgt tcagcatgac tgtaagtttg tccagtccct tcaagctccg ttatttcccc
    42361 aaatgttgaa attcccaatt ctaccattgt gattctcctt gtctatctct gtccttcaat
    42421 ttgaaaaatt attctaacac tcaatgaaaa tcaaagagca aactaggaag ctagccgcag
    42481 gttgctcaaa acagtgtttt gaggttgtgg atagaactga cgaaatcagt aaccatacct
    42541 acggcaaggc gacgttgacg tggtttgaag agattttcta agagtataac acgaatcttg
    42601 agtacaagca accaatttgc tcacaagaaa aagcctagat aactagactt ttttagctta
    42661 ttctaccgtt actgacttgg caaggttacg tggtttgtcc acatcgaggc cacggtggag
    42721 ggttgcaaag taagcgacta attgcgttgg tacgaccatt gaaattggtg agaggtatgg
    42781 atgtacggtc gtaaggacga tatcgtcggt atctttggct acattctctt ctgcgatagt
    42841 gaggactttg gcaccacggg ctgcgacctc ttggatattt ccacgagtat ggttggcaag
    42901 aactggatct gacaagagag ccaaaacagg cgttccttct tcaatcaagg caatggttcc
    42961 gtgcttgagt tctcctgccg caaaaccttc acactggata taagaaatct ctttgagttt
    43021 gagacttgct tccatggcta cgtaataatc ttgaccacgt ccgatgtaaa aggcgttacg
    43081 agttgtttca agaagttcac gaaccttggc ttcaatggtt tctttctctg aaagagttga
    43141 ttcaatagac tgagctacga ttgacaattc atgaaccagg tcaaaggctt gcgctttagc
    43201 attaccattt gcttctccga ctgcttttgc aaggaaggca agggctgcga tttgcgctgt
    43261 ataggcttta gttgatgcca cggcaatttc aggacctgcg tgaaggagca tggtatagtt
    43321 ggcttcacgt gagagggttg aacctggaac atttgtcact gttaagcttg gaattcccat
    43381 ttcattagcc ttgaccaaaa cttgacgact atccgctgtt tcaccagatt ggctgataaa
    43441 gatgaagagt ggtttcttgc tgagaagtgg cataccgtag ccccactcag atgagattcc
    43501 aagttcaact ggtgtatctg tcaattcttc caacatcttc ttagaagcaa atcctgcatg
    43561 gtaagatgtt ccagctgcaa ggatgtagat gcggtctgcg tcttgaacag ccttaatgat
    43621 atctgggtct acgacaactt gaccagcctc atctgtgtag gcttggatga gtttccgcat
    43681 aacagttggt tgctcgtcaa tttccttgag catgtagtaa gggtaagttc ccttaccgat
    43741 atctgacaag tcaagttcag cagtgtagct agcacgctca cgacgatttc catcatagtc
    43801 ttgaacttcc acgctatcag ccttgacgat taccaactct tggtcatgaa tttccatgta
    43861 ttggttggtt tcacgaatca tagccatagc atctgagcag accatattgt agccttcccc
    43921 aagaccaatc aaaagtggag atttgttttt cgctacatag atgacatctg gattttcaga
    43981 gtcaatcaag gcaaaggcat atgaaccacg gataatatga agagcttttt taaaggcttc
    44041 aagaactgag agaccgtctt cttccgcaaa ttttccaatc aaatgtacgg cgatttccgt
    44101 atctgtttgc cctttgaagt ggtgcccagc aaggtattct tctttaattt caaggtagtt
    44161 ttcaatcacc ccattgtgaa ccaagacaaa acgttctgtc tcagagcggt gtgggtgagc
    44221 attgtcttcc gttggtttcc catgagttgc ccaacgagta tgtccgatac cagttgttcc
    44281 ctcaacacca gctgtcttgg cagacaattc tgcgatacga ccaacagcct tcaccaaatg
    44341 gttatcagca ccatctagga caaaaattcc cgcagaatca tagccacggt attcaagctt
    44401 ttcaagccct tgaatcaaaa tatcagttgc atttgtgttt ccaacaacac caacaattcc
    44461 acacatagta tatacgacac aggcaagctg tgctttctcc ttaaaattgg tatagtctaa
    44521 ttcatctttt atagaatcag caaaaacagt atatacttgt ttctttcact tgtcaagagt
    44581 aaaaattggt atagttcaaa ttaagctcct gtaagcaaaa aactctgacc gattgggata
    44641 atcagtcaga gtccttttta aaatccatta ttatcgctta attttttgaa ccagtggcct
    44701 gatttcttca gacgacgttc ttgcgtttcc aagtctaatt cgaccaaacc atagcgattt
    44761 ttatagctgt tgagccatga ccagcagtca ataaaggtcc aaatcaagta gcccttacag
    44821 ttggcaccat cttcaatggc acggtgaagt tcacgaagat gaccttttac aaagtcaata
    44881 cggtaatcat cttgaatcat tccatcttga cggaattttt cttccccttc aacacccata
    44941 ccattctcag tcaacatcca ttcaatattg ccataatttt ccttgatatt ttgggcgatg
    45001 tcataaatcc cttgctcata aatctcccaa ccacggtgag aattgatttt acgtccaggc
    45061 atcacataag gctcgtaaaa atgttctggt aagagtggac tctctggatg cttagcaaat
    45121 cgaggagcca taacacgcaa aggttgatag tagttcacac caaggaagtc caccgtatta
    45181 tcatgaatga gttccaactc ttcctctgta gcatcaggta aaagaccgtg ttcatgcaag
    45241 atttctacca actcctgtgg ataagtcccc aagacagatg gatctaagaa agattgggcc
    45301 tgaaaaaggg ccgcaatacg agctgccttg acatcagcag gatgctggct acgtggataa
    45361 gccggtgtca agttgaggac aatcccaatc ttggaatcag gcaaaagttc atggcaagcc
    45421 ttaacagccc ggctgctggc caattgtgta tgataggcta ccttaacagc tgcctctgca
    45481 tccaccttat gtggataatg ggcatcataa aaataaccaa attctacagg aacgatgggc
    45541 tcgttaaagg taatccattg atccactaaa tctccataag tctcaaaaca aaaacgagca
    45601 tagtcttcat aggctgagac tgtcgcctta ttttcccaac catcaccatc ctcttgaagg
    45661 gcaaaaggta aatcaaaatg atagagattg actaacagac gaattccttt agccttaata
    45721 gcctcaaaga ccttacgata aaaatccaca ccttgagggt tgacttttcc acagccttgt
    45781 ggaaaaatcc gtgaccactg aatagaagtc cgaaagactg tgtgaccagt ctctaacaaa
    45841 agctcaatat cccgctccca attttcataa aaagtcgatg tcttatctga accaatccca
    45901 ttatagtaac gatttggctc cacttggaac cagtaatccc agagattgtc tcccttaccg
    45961 tcaccagcta cacgtccttc tgtctgcggt ccagaagtag aggatcccca gacaaaatcc
    46021 tttggaaatc ttagcataac atttacctct ttatctactc atttctccca ttatacagaa
    46081 aaaacaaggt aaaaactagt tacatttttt ccttgttttt cttctgatta tagtttttat
    46141 ttcttgctta ggatttcaag cgtttcaagc acgttatctg catgaacctc aatggtgtca
    46201 ccagttgcct tgatcttaac ttctacaatg ccatcggccg cttttttacc aacagtgata
    46261 cggattggaa gaccaatcaa gtcactatcg ctaaatttaa caccgacacg ttcgttacgg
    46321 tcatctgtca agacttcata accagctccc atcaagcttg cttcaagttt ttctgtcaag
    46381 gcttgcgctt cttcatcctt gacattgaca gtaatcaaat gcacatcaaa tggtgccaat
    46441 tctttaggga aattgattcc ccaagcgtaa cggtattcac cttttggcgt tttgttaaca
    46501 aagaggcgag cgtgttgctc catcactgct gaaagaagac ggctgacacc gataccgtaa
    46561 catcccatga tgattggcac agcacgacca ttttcatcca agacatctgc tcccatgctt
    46621 gctgagtagc gagttccgag tttgaaaata tgaccgatct caataccacg cgcaaagtta
    46681 aggacacctt gtccatctgg ggaaatttca ccctcacgaa cttcacggat atccacatat
    46741 tctgcagtaa aatcacggcc tgggttcaca ccagtcaagt ggtagccatc ttcgttagca
    46801 ccgacaactg cattgcgaac atcttgcacc ttacgatctg caataatttt aatattctct
    46861 ggcaaaccaa ctggtccaag tgaaccaaat cctgcttgaa caacattcgc cacttcttct
    46921 tcgctagcaa cgtcaaagaa atctgctcct aagtggtttt tcaacttgac ttcgttgagt
    46981 tggtcatttc caactagaag ggctgcaaca agctcaccat ctgcaatgta gaagagggtt
    47041 ttaatcgttt gttcttctgg aacattgagg aaagctgcaa cttcatcaat tgatttaaca
    47101 tctggcgttg caacacgagt aacttcttct tcagcgacaa cacggttgct tggtttgtac
    47161 tcgtttgttg ccatttctaa gttagctgca tagctagact cacttgagta agcaatggta
    47221 tcttcaccag agactatcca tttgagcaat tctgccttga tttcttcttg cacttctgca
    47281 ggaatttcgt caaatgaggc aactgacttg tccaagacaa cccagcggtc aaggtctgta
    47341 cgagcagatg taatggccat aaattcttgg ctatccttac cacccatggc tccaccgtca
    47401 ccaataatag ccttgaagtc taaaccacta cgagtgaaaa tacgctcata ggctgctttg
    47461 tactcatcat aaacactatc caaactatca tagttagcgt ggaaactata agcatccttc
    47521 atgataaact cacgtgtacg aagaagtcca ttacgtgggc gtttttcatc acgatacttg
    47581 ggctgaattt gataaaggtt gagtggcaat tgcttgtaag atttaacaga atcacggaca
    47641 atagctgtaa aggtttcttc gtgagttgga cctaagataa agtctgattt ttcacggttt
    47701 tttagtttgt aaaggtcttc accataggtt tcgtaacgac ctgattcacg ccacaattct
    47761 gcactaagaa gggctggagc caacatctca acagcaccaa tcttttcgaa ttcttggcgc
    47821 atgatgtttt tagctttttc aatcacacga ttggcaagtg gtagataaga ataaacacct
    47881 gctgaaactt ggcgaacata accagcacgc aacataagag catggctgat aacttgagca
    47941 tcgcttggca tttcgcgaag cgttgggata ggcattttac tttgtttcat aatattcctc
    48001 gattatctaa aaaagagtcg cataatgtca ttccaagtca cagcaatcat caagacaacc
    48061 atgatgacca ctccggccaa ggtgacatag gtttcaattt cttgtttcaa tggtttgcgg
    48121 cggatggctt ctaggatatt gagcacaatc ttaccaccat ccaaggctgg aatcggaata
    48181 agattaaaaa tcccaatatt gatggaaatc attgccaaga agtacaagat attttcaatt
    48241 ccatttttag cagcatcact acttgcctta aagatagcaa caggtccacc caacttgttc
    48301 aaatccggtt ggaaaatcag atttttcaga gctgagagaa ttcggagagc tgagtcagca
    48361 gcagttgtaa aaccacctac aaacatggat agaaaatctg acttaacccc cggttgaaca
    48421 cctagaaggt aacgaccttg actatcttcg ggtgtaacag tgacttgttt gtcactcccc
    48481 ttttcagaaa tagtcacatc caaagtcggt gccgtcttat ctttggtttc tgtttccaca
    48541 gcttggatca agctttccca gttgctaacc tcatgtgagc caatcttggt aatttgtgcc
    48601 gtttctggta ctcctacctt ggccaaggca ccttggggca tgatatggaa ctgattggta
    48661 tcaacatctc tgacaccacc ctgcataaag attaaaaccc aaaaaacaac gacacctaag
    48721 ataaaattgt tcataggacc tgcaaaattg gtaatcagtt tgccccagat agtcgcattt
    48781 tgatattgaa catctaaagg tgcaatccga acctcagtac catctgcttc cacaaccgtt
    48841 gcatcgtgat ccactgcaaa tgttttttct tcttccagaa ccaatccttt gataaagagc
    48901 ttgtcttcaa aatcaaactg ggtcacctgc atagggaggg ctgtttgatc caatttttta
    48961 cctgagagat tgatgcgttt aaccttacca tcatcagcaa gtgtcaaact aacaggcgtt
    49021 cctgtcttga tttcagttgt atcatcaccc caaccggcca tgcggacata gccacccaga
    49081 ggcaagattc gaatggtata ggccgttcca tccttgccaa tgtgagcaaa aattttaggt
    49141 cccataccga tggcaaattc acgtactaaa atccctgatt tcttggcaaa gtagaagtgc
    49201 ccgaactcgt gcaccactac aataatcccg aaaaccagaa taaaggttaa aattccgagc
    49261 atagcgtttc ctccgtcttt tgattaaaag agtccaaata agtacatgat tggaaataca
    49321 agcaacatac tatcgaaacg atccaaaaca ccaccatgtc cagggataaa tttcccagaa
    49381 tccttaacac caaaatgacg tttgatcgaa ctttctagta aatcaccaaa ttgtccagca
    49441 atgctaaaga aaatagcaaa gactgacatc ttgtaaattc catatggaag agcaactgta
    49501 ctgtcaacta tcataaagat aatggttact aaaattgctc ctaaaatacc acccaaggca
    49561 ccctcaaggg ttttattagg cgataccctt ggtgctaact ttcgtttccc atagttcatc
    49621 ccaacaagat aggcaccact gtctgtcgcc cagacgatac acaaggctaa gagagccttg
    49681 tccaaacctg caacacgagc atctagtaaa gcattaaatc caaagcccac gtagaagctc
    49741 atagcaagag ggaaaaccgc atcctcaatc gtataagact tgctaaaaac ggtcgttcct
    49801 aacatgattg aaatcaaaac actataggca accacattcc catcaactgg caaaaaagtc
    49861 aggtaattct ccaagggaat ggtcaatgca aaggttgcaa agagggtcaa gaggccctcc
    49921 atcgtcatgg tctctagacc tctcatcttc aaaagttcat gcatggctag catggctatg
    49981 attccgattg ctatctgaag caagaggccc ccaatcatta aaattggtag gaaaatagcc
    50041 agggcaatcc ctgcaaacaa ggttcttttc tgtaaatcct gggtcatatt tcctcctaaa
    50101 ctcctccaaa tcggcgatga cgacgattat aggcaagaat agcttcctgc aaggccgctt
    50161 cgtcaaaatc aggccataag gtgtccgtaa aataaagctc actataggct ccctgccatg
    50221 gaagaaaatt gctcaaacgt aattctccac tagtacggat aatcaagtct gggtctcgta
    50281 agtccttagg caaatgctga gtaaagagat agttaccaat caattcctct gtgatgtcac
    50341 ctgggttgat tttggcatct aaaacatcct gggaaatcaa cttaagcgcc tgtgtaatct
    50401 cagcacgtcc accatagtta agagcaaaat taagaatcaa tcctgtgttg ttcttagtca
    50461 attcctcagc cttggttaaa gcttcaaagg tttgcttagg caggcggtct gtctccccaa
    50521 tcatttgaat cttaacatta ttcgcatgta gttccgggac ataattatca taaaactcta
    50581 ctggcaagtt catgataaac ttgacttcct gatctggacg ggtccagttt tccgtagaaa
    50641 aagcatagac cgtaataacc ttgacgccca gtttgttggc tgccttggtc acggtttgca
    50701 atgcttccat gcccgcctta tgtccaaaaa ctcgcggttg catacgtttt ttagcccaac
    50761 ggccattgcc atccatgatg atgccgatat gagcaggaac ctgtgtcgga acctctactt
    50821 ccacagcctt atctttctta aaaaatccaa acatgatctt attcctattc aaaaatctat
    50881 cgtttcatta taccatattt ccccattttc ttctatcact aagctattta ttctcaggca
    50941 ccatgcccat ttttcaaaaa aataagccgc ctgattgggc gactttattt ttatagggag
    51001 attattatga aaaagtttta ggagtttaag ttaaggtctt cttaacttat gaacttagtg
    51061 tacactccct agcttaaagt ttccttaagt atttttaaaa atcaaatttt tccatttctc
    51121 ctgccaattt ttcttggata aacgtgtttg atagagttcc attcggtctt cattttctaa
    51181 gaaatgagga gttggacgaa cttgaaaatt caaaatatcc tccaaaccat aaggtgcata
    51241 gagttcaaaa tctgattctt cattcaagcg cagtccaact gccgtacacc gttctggata
    51301 cttactcata gcatcacgag aactggtata ggaagcagtg tgaggactgt gctgatgcat
    51361 atagacctga tttttcaatt cccactggta ctgaggaaaa tcctctctca gctttttctc
    51421 cagtaataag gtttcctcat aagaaaaatc tggatcaaag aaaatcacat ctatatctgt
    51481 ttcatgatca aaaggggatt tgtctgacaa aagattccag atgaaatttc tgacagaacc
    51541 tgctgccaac tacgagtctt tcaaaccaag gtctcggatg atcgtcagaa tggccatcat
    51601 atctggattt tctctaaaag cctctaagat ttcttgctta tttttcactg tattcataac
    51661 ctaagtgctc atatgcctta gcagtcgcca cccgtccaga ccgtgtccgc atgataaaac
    51721 ctttttgaat caagtaaggc tcatacatgt cttcaactgt ctcacgctct tcggcgatat
    51781 tgacagaaag agttcctaga ccaacaggtc ctccactgta catctcaatc atggtacgaa
    51841 ggattttttg atccacatag tccaaacctt catggtcaac atccagcata gtcaaagcct
    51901 tatcggtaat aatatcatcg attaccccat tccccattat ctgggcaaaa tcgcgcacgc
    51961 gcttgaggag acgattggca atacgagggg ttccacgact acgtagggcc aactcagatg
    52021 ctgcctcatg agtgatttcc atctcaaaaa tatctgccgt ccgctcgaca atttctgtca
    52081 agtcagcatg ggcataatac tccatatgac ctgtaatccc aaaacgtgcc cgtagtggat
    52141 ttgagagcat accagcccga gtcgtcgcac caatcaaggt aaaaggaggc aactccaaat
    52201 gaacactgcg actgccttca ccagccccaa tcataatatc gatgtagaag tcctccatgg
    52261 cactataaag cacttcttcc actgacatgg gtaagcgatg aatctcgtca ataaagagga
    52321 catctccagg ctctaactca ttcaaaatcg ctaccaaatc acccgctttt tcgataacag
    52381 gaccagacgt ttgcttgaga ttgactccca gttcattggc aatgacaaaa gccatggttg
    52441 ttttcccaag ccctggaggg ccaaataaga gcacatgatc cagcgcttca tcccgcattt
    52501 tagcggcttc gataaagatc tgaagctgat ccttaacctt atcctgacca atatattcac
    52561 gtaaatactg aggacggagc gtgcgttcta ctaactcctc atcacccatc atctcattat
    52621 ctaaaattct actcatggct ctattatatc aaaaaaacaa gccacaaaca aaaaagccac
    52681 ctgattgggt gactcctaag tttagcactt atgtggtata atattatacg acactgctac
    52741 accgcctacg aaaggaggtg agatagccca tgatggaatt agtactcaaa actatcatcg
    52801 gaccaattgt ggtcggtgtc gttcttcgta tagtcgataa atggctaaac aaggacaaat
    52861 agtgtcaaaa aaagacctca agcttatttg gtcgtgagct tggggtcttt tctagcctat
    52921 gatatagaac tagtactcaa ttccttttta ttatcccata gttcacgaat tttgtcaaag
    52981 gtttacattt tcttcaaccg ctgtacgaca agacggttaa gattaagaga acgttaggga
    53041 ttctatcaat ttcatagaaa ttttgatttc gtaaacgaag agacaatctt acatgtcact
    53101 tctcatttaa tacgccacta ctagacaagc aaaatcatta ttacagtagt tccagttctt
    53161 caattaacag tcacttacaa tcaaattgag tttgaactag ctgaagcgac cacagaccta
    53221 tttcttagtt atattcgcta aaaaagtccc cgccaattcc ccgaccaaaa t
//
LOCUS       DAVKJJ010000006        51851 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000006 DAVKJJ010000000
VERSION     DAVKJJ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 51851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51851
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.006"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            1..980
                     /locus_tag="VW144_000342"
                     /pseudo
     CDS             1..980
                     /locus_tag="VW144_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1069..1758)
                     /gene="rplA"
                     /locus_tag="VW144_000343"
     CDS             complement(1069..1758)
                     /gene="rplA"
                     /locus_tag="VW144_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU9599841.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(1966..2391)
                     /gene="rplK"
                     /locus_tag="VW144_000344"
     CDS             complement(1966..2391)
                     /gene="rplK"
                     /locus_tag="VW144_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU9599842.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            2523..2660
                     /locus_tag="VW144_000345"
     CDS             2523..2660
                     /locus_tag="VW144_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9599843.1"
                     /translation="MKIKEQTRKLAAGCSKYSFEVADKTDEVSSKHCFEVVNRTDAVS
                     R"
     gene            complement(2700..3416)
                     /locus_tag="VW144_000346"
     CDS             complement(2700..3416)
                     /locus_tag="VW144_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEU9599844.1"
                     /translation="MKKRYIVLSGLLAVTLAACSQEKAKNEDGTAKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(3442..3903)
                     /locus_tag="VW144_000347"
     CDS             complement(3442..3903)
                     /locus_tag="VW144_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9599845.1"
                     /translation="MCLICQRIEWIKAGENPYFVKELETGYVVIGDHQYFKGYTLFLA
                     KEHVTELHHMETSVKLRFLEEMSLVQEAVAKTFKAEKMNIELLGNGDAHAHWHLFPRR
                     SGDMRGHGLNGRGPVWWVPWEEMAAEDCQVKSHELEQMIKALSDELEKYTV"
     gene            complement(3905..4942)
                     /locus_tag="VW144_000348"
     CDS             complement(3905..4942)
                     /locus_tag="VW144_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEU9599846.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTETGFIKSRHLDDKVSAAILLNLLRVYKEEGIELPVTTHFAFSVFEE
                     VGHGANSNIPSQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVVLA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLKSALVD"
     gene            complement(5040..6365)
                     /gene="brnQ"
                     /locus_tag="VW144_000349"
     CDS             complement(5040..6365)
                     /gene="brnQ"
                     /locus_tag="VW144_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEU9599847.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(6536..6628)
                     /locus_tag="VW144_000350"
     CDS             complement(6536..6628)
                     /locus_tag="VW144_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9599848.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(6671..6823)
                     /locus_tag="VW144_000351"
     CDS             complement(6671..6823)
                     /locus_tag="VW144_000351"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9599849.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            complement(6983..7561)
                     /locus_tag="VW144_000352"
     CDS             complement(6983..7561)
                     /locus_tag="VW144_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU9599850.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFLLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(7615..9015)
                     /gene="pepV"
                     /locus_tag="VW144_000353"
     CDS             complement(7615..9015)
                     /gene="pepV"
                     /locus_tag="VW144_000353"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEU9599851.1"
                     /translation="MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLQGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(9028..9633)
                     /locus_tag="VW144_000354"
     CDS             complement(9028..9633)
                     /locus_tag="VW144_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU9599852.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIADRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            9804..10604
                     /locus_tag="VW144_000355"
     CDS             9804..10604
                     /locus_tag="VW144_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9599853.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(10786..11619)
                     /locus_tag="VW144_000356"
     CDS             complement(10786..11619)
                     /locus_tag="VW144_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU9599854.1"
                     /translation="MNHKIAILSDIHGNATALEAVIADAKNQGVSEYWLLGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDRVLEALDGQYGLEDPQEVQLLRMTQYLMERMEPA
                     TIVWLRSLPLLEKKEIDGLRFSISHNLPDKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELVNILFRKV
                     AYDYEAELEFAKSKGLPFIEMYEELRREDNYQGHNLELLASLIEKHGYVEDVKNFFDF
                     L"
     gene            complement(11609..13456)
                     /gene="uvrC"
                     /locus_tag="VW144_000357"
     CDS             complement(11609..13456)
                     /gene="uvrC"
                     /locus_tag="VW144_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU9599855.1"
                     /translation="MNNLIKPKLELLPTSPGCYIYKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNEHYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKNDEAYFKSMAQEVSDFLKGQDDKIIDDLKSKMAVTAQSMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGRPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     GVDEIVEVGVPRAVAEAVQRKLNPQETEILLQVAEERVDYQTEGNHNEP"
     gene            complement(13495..14196)
                     /locus_tag="VW144_000358"
     CDS             complement(13495..14196)
                     /locus_tag="VW144_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEU9599856.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETNE"
     gene            complement(14275..15507)
                     /gene="murN"
                     /locus_tag="VW144_000359"
     CDS             complement(14275..15507)
                     /gene="murN"
                     /locus_tag="VW144_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEU9599857.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQDLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQNKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(15512..16732)
                     /gene="murM"
                     /locus_tag="VW144_000360"
     CDS             complement(15512..16732)
                     /gene="murM"
                     /locus_tag="VW144_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEU9599858.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQTIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMIWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(16844..17623)
                     /locus_tag="VW144_000361"
     CDS             complement(16844..17623)
                     /locus_tag="VW144_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU9599859.1"
                     /translation="MAVMKIEYYSQVLDMEWGMNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(17692..19353)
                     /locus_tag="VW144_000362"
     CDS             complement(17692..19353)
                     /locus_tag="VW144_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9599860.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(19528..21750)
                     /gene="recJ"
                     /locus_tag="VW144_000363"
     CDS             complement(19528..21750)
                     /gene="recJ"
                     /locus_tag="VW144_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU9599861.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIAFMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAIVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            22027..22785
                     /locus_tag="VW144_000364"
     CDS             22027..22785
                     /locus_tag="VW144_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9599862.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLETVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLAHDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            22796..23590
                     /locus_tag="VW144_000365"
     CDS             22796..23590
                     /locus_tag="VW144_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9599863.1"
                     /translation="MKKKFFLSALLISLFGLAAAKPVQADTSIADIQKRGELVVGVKQ
                     DVPNFGYKDPKTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIA
                     TFTITDERKKLYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITE
                     LGKKKGLKFKFVELGSYPELITSLHAHRIDAFSVDRSILSGYTNKRTALLDDSFKPSD
                     YGIVTKKSNTELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            23603..24280
                     /locus_tag="VW144_000366"
     CDS             23603..24280
                     /locus_tag="VW144_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9599864.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            24290..24949
                     /locus_tag="VW144_000367"
     CDS             24290..24949
                     /locus_tag="VW144_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9599865.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALVEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            25207..26397
                     /locus_tag="VW144_000368"
     CDS             25207..26397
                     /locus_tag="VW144_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEU9599866.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTIKNSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(26718..27599)
                     /locus_tag="VW144_000369"
     CDS             complement(26718..27599)
                     /locus_tag="VW144_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEU9599867.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(27740..29068)
                     /gene="vncS"
                     /locus_tag="VW144_000370"
     CDS             complement(27740..29068)
                     /gene="vncS"
                     /locus_tag="VW144_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEU9599868.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMYLDAQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            complement(29065..29721)
                     /gene="vncR"
                     /locus_tag="VW144_000371"
     CDS             complement(29065..29721)
                     /gene="vncR"
                     /locus_tag="VW144_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEU9599869.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASIAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(29770..29853)
                     /gene="pep27"
                     /locus_tag="VW144_000372"
     CDS             complement(29770..29853)
                     /gene="pep27"
                     /locus_tag="VW144_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEU9599870.1"
                     /translation="MRKEFHNVLSSGQLLTDKRPARDYNRK"
     gene            complement(30033..31412)
                     /gene="vex3"
                     /locus_tag="VW144_000373"
     CDS             complement(30033..31412)
                     /gene="vex3"
                     /locus_tag="VW144_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEU9599871.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(31464..32111)
                     /gene="vex2"
                     /locus_tag="VW144_000374"
     CDS             complement(31464..32111)
                     /gene="vex2"
                     /locus_tag="VW144_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEU9599872.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(32124..33401)
                     /locus_tag="VW144_000375"
     CDS             complement(32124..33401)
                     /locus_tag="VW144_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9599873.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
     gene            complement(33758..33928)
                     /locus_tag="VW144_000376"
                     /pseudo
     CDS             complement(33758..33928)
                     /locus_tag="VW144_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(34071..34670)
                     /locus_tag="VW144_000377"
     CDS             complement(34071..34670)
                     /locus_tag="VW144_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9599874.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEETGYNNNSSLYTTIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEITVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNHP
                     AGAPTFAKGEGEHANDIVATYSDGTTYYAPLNDVTKYAR"
     gene            complement(34912..35796)
                     /locus_tag="VW144_000378"
     CDS             complement(34912..35796)
                     /locus_tag="VW144_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEU9599875.1"
                     /translation="MREKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLTSW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(35800..36186)
                     /locus_tag="VW144_000379"
     CDS             complement(35800..36186)
                     /locus_tag="VW144_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEU9599876.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(36179..37522)
                     /locus_tag="VW144_000380"
     CDS             complement(36179..37522)
                     /locus_tag="VW144_000380"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU9599877.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLDLGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMTKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLAAQGIKLLDTKDGVRWTRD"
     gene            complement(37604..38488)
                     /locus_tag="VW144_000381"
     CDS             complement(37604..38488)
                     /locus_tag="VW144_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9599878.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFEREIASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(38500..39117)
                     /gene="cysE"
                     /locus_tag="VW144_000382"
     CDS             complement(38500..39117)
                     /gene="cysE"
                     /locus_tag="VW144_000382"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEU9599879.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(39133..41346)
                     /gene="pnp"
                     /locus_tag="VW144_000383"
     CDS             complement(39133..41346)
                     /gene="pnp"
                     /locus_tag="VW144_000383"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU9599880.1"
                     /translation="MAKQVFQTTFVGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELITFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDHKPKKEFTETPKDSE"
     gene            complement(42031..42897)
                     /gene="metF"
                     /locus_tag="VW144_000384"
     CDS             complement(42031..42897)
                     /gene="metF"
                     /locus_tag="VW144_000384"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEU9599881.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(42961..45210)
                     /gene="metE"
                     /locus_tag="VW144_000385"
     CDS             complement(42961..45210)
                     /gene="metE"
                     /locus_tag="VW144_000385"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU9599882.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVSAYQEVFAKLAELGTTRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLTVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDALKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEVILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            complement(45471..45737)
                     /locus_tag="VW144_000386"
     CDS             complement(45471..45737)
                     /locus_tag="VW144_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9599883.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGKTSPQLDTVLKVLASLGKTLAVVPLEQGKS"
     gene            complement(45863..46266)
                     /locus_tag="VW144_000387"
                     /pseudo
     CDS             complement(45863..46266)
                     /locus_tag="VW144_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            46422..47423
                     /locus_tag="VW144_000388"
     CDS             46422..47423
                     /locus_tag="VW144_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9599884.1"
                     /translation="MIKTFLSALSVILFVIPIIAYSFFPSSNLNNWLSTRPILAQIYA
                     FPLATATMASILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNTTIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            complement(47491..49896)
                     /locus_tag="VW144_000389"
     CDS             complement(47491..49896)
                     /locus_tag="VW144_000389"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU9599885.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVIIGTDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDN
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SRVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VRVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKYDGTAGELTATQKLRRQVRTIAE
                     GVGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPREELPNEINSFAFALTGLVAEKDFQTVAVPVDFFYA
                     KGILEALFARLGLQVTYTATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEAALQPAAPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(49973..50482)
                     /locus_tag="VW144_000390"
     CDS             complement(49973..50482)
                     /locus_tag="VW144_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9599886.1"
                     /translation="MIRKVEVADVEVLAKIAKQTFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            complement(50482..51528)
                     /gene="pheS"
                     /locus_tag="VW144_000391"
     CDS             complement(50482..51528)
                     /gene="pheS"
                     /locus_tag="VW144_000391"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU9599887.1"
                     /translation="MSTIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
BASE COUNT        14627 a        11789 c         9741 g        15694 t
ORIGIN      
        1 attgctcaaa agttaattga aaagatttct atgactgata ttgcccatca gctttccatc
       61 tcaacttcaa ctgttattcg taagctcaat gattttcact ttaaacatga tttttcttgt
      121 cttcctgaga ttatgtcttg ggatgagtat gcttttacaa agggaaagat gagcttcatt
      181 gcgcaagatt ttgataatct taatattatc actattctta aaggcagaac acaagctgtc
      241 atccgaaatc actttcttcg ctacgataga gctgtccgat gtcgcgtcaa aatcattact
      301 atggatatgt ttagccttta ctatgacttg gctaaacagc ttcgctttca aatttctagg
      361 ctcaggctga aacagtctcc cagactgttt cactcccgaa tgctaaaatc gttcttgaac
      421 gctttcacat tgtacaacat cttagccgtg ctatgagtcg tgtgcgtgtc caaatcatga
      481 atcagtttca tcgaaaatcc catgaataca aggctattaa gcgctactgg aaactcattc
      541 aacaggatag ccgtaaactg agtgataagc gattttatcg tcctactttt cgtatgcact
      601 taaccaataa agagatttta aacaagcttt tgagctattc ggaagacttg aaacaccact
      661 atcagctcta tcagctcttg ctttttcact tccagaataa ggaaccagag aaatttttcg
      721 gacttattga ggacaatctt aagcaggctc atcctatttt tcagactgtc tttaaaacct
      781 tcctcaaaga taaagaaaag attgtcaacg cccttcaact acactattct aatgccaaac
      841 tggaagcgac caataatctc atcaaactta tcaagcgcaa tgcctttggt tttcgaaact
      901 ttgaaaactt caaaaaacgg atttttatcg ctctgaatat caaaaaagaa aggacaaaat
      961 ttgtcctttc tcgagcttag ctttttttca acccactaca gttgacaaag agccaaaaaa
     1021 agttgagaca tagcgtcttc aactttttta cattacttta actactgatt aaagtgagtt
     1081 tacatcaact ttgataccaa caccttgagt agttgtgata gtcaagtttg ttacgtaagt
     1141 tcctttagct gtagctggtt ttgctttttg gattgtttcg ttgaaagctt taaagttttc
     1201 aaccaatttt tcagcttcaa atgatacttt accgatgatt gcttgaacgt tacctgcacg
     1261 gtcagcacgg taagtgattt taccaccttt agactcttca actgctttag caacatccat
     1321 tgttacagta ccagttttag ggtttggcat caagttacgt ggtccaagga cacgtccaag
     1381 acgtccaaca agagccatca tatcaggtgt agcgataact acgtcgaagt ccaaccaacc
     1441 gtcgttgatt ttagcaacaa ggtcatcttc accaacaaag tctgcaccag cagcttttgc
     1501 ttcttcagct tttgcaccac gtgcgaaaac aagaacacgt gaagttttac cagtaccgtt
     1561 tggcaatacc attgctccac ggatttgttg gtcagctttt ttaacgtcga tgttcaagtt
     1621 gtaagcaact tctacagttg cgtcaaattt tgcaaagtta gtttcttttg caagtgctac
     1681 agcttcttct acgctgtatg cttttgtgct gtcgattttc tcaagagcag cacgaagttg
     1741 tttgcttttt ttagccattt tctattctcc ttgtaagtgg ttcaatcgat tttcatctcc
     1801 cacgtcactt ctcgaaatga tgaagtcttg cgggttttca gtctactatt tttctcttcc
     1861 tacggcctta agctcccttg ctgtacccaa gtacaagcct cagtcacttg cctagtatga
     1921 aaagcaacta atagactgct gtgagatttt ctactgtgtt attgattagt caacaacagt
     1981 gaatcccata gaacgagcag taccttcgat catacgcatt gcagactcta cgtttgctgc
     2041 gttcaaatct ggcatcttag tttctgcaat ttcttgtact tgtgcacgag taactgtagc
     2101 aactttagtt ttattaggtg tacctgatcc tttttcaaca cctgcagctt ttttcaaaag
     2161 aacagcagct ggtggtgttt tcgtaacgaa agtaaatgat ttatcttcgt aaactgagat
     2221 aacaactgga atgatcatac cagcttggtc agctgtacga gcgttgaact cttttgtgaa
     2281 tcccatgatg ttgataccag cttgaccaag agcaggtcca accggtggag ctggtgtagc
     2341 tttaccagca gggatttgca attttacaag tttttcgact tttttagcca tttttaaatc
     2401 ctcctttgtg gttttggcgg taattagaga tttttacctc ccacaagtat gtttttcgca
     2461 tacccattca ttatacacta tttatctgga attgcaagag aaaagtttat tttttatact
     2521 caatgaaaat caaagagcaa actaggaagc tagccgcagg ctgctcaaag tacagctttg
     2581 aggttgcaga taaaactgac gaagtcagct caaaacactg ttttgaggtt gtgaatagaa
     2641 ccgacgcagt cagtaggtaa gacgacgctg acgtggtttg aagagatttt cgaagagtat
     2701 taatcgacgt agtctccgcc ttcaaagcca tagtattctt ccaaactgag accacttgca
     2761 gcaatttctt tagcttcttt tcctacataa cgcaggtgcc attcctcagc catatagcct
     2821 gtttcctttt ccttgccttt gagataacgg acaacaaagc cataatcagc tgcatgatcc
     2881 aagagccatt gggctgcttt ttcttctgtc accaaatcac catcagtccc aatcacatca
     2941 aaggccaagc ctgtctggtg ttcgctatag ccaggacggg cagagtaacg gtcagctgct
     3001 gcctttccat cttggttgac ataatcttga tagagcttgg tctgagtttc ataacttcta
     3061 aaaccactgt aatgatcact aatagggaaa cctgcctctt gcatcgcttt gatgagtttg
     3121 accaactcag ccttggctgt tggattttcc ccgggattat agtctttcga caaaggataa
     3181 tgtttattgg ctacgatgat ttcgtcgtat tttccttgga tgctgtagta atcaccttta
     3241 ttgaccactt ctgctttttt ctgggcagcc ccttgagact tactgccgac tgtcccatca
     3301 gctttggctg tctgttctgt cttagctgtg ccatcttcat tttttgcttt ttcttgtgaa
     3361 caagctgcta gggttactgc cagcaaacca gataaaacga tgtatctttt tttcattttt
     3421 tcttcctcta ttcttcctct cttagaccgt gtacttctct aattcatcgg ataaggcttt
     3481 aatcatttgt tccaactcat gggatttcac ttggcaatct tctgccgcca tttcttccca
     3541 gggcacccac cagactggac cacggccatt caatccatga cccctcatat cacctgaccg
     3601 tcttggaaag agatgccagt gagcgtgggc atctccattt cctagaagtt caatgttcat
     3661 cttttcagct ttaaacgttt tggcaacagc ttcttggacc aaactcattt cctctagaaa
     3721 acgaagtttt acagaagtct ccatatgatg gagttctgtg acatgctctt ttgctagaaa
     3781 taaggtatag cccttaaagt attggtggtc tccaataaca acatagcctg tttctagttc
     3841 ttttacaaag tagggatttt cccctgcctt gatccattca attctttgac aaatcaagca
     3901 catattagtc taccaatgca ctcttaagat aagcatcgac catacgctca gtcgccacta
     3961 ctgaatcaat gtgtgttcgt tcgtaagagt gacttgactc aataccagct ccaaggagag
     4021 catgctttac ttctgctcct gcagacatag ctgccgaagc atccgaaccg taaaatggat
     4081 aaatgtcgag cttaaatgga atatcttgct ccttcgccaa aactaccaag tgttgacgga
     4141 agtcatagtg atagggacct gaagcgtcct tgacacagat agacactgta tactcatccg
     4201 tctgctggtc atctcccata gctcccatat ctacagccag atattctact acctgacttg
     4261 gaatattgga gtttgcaccg tggcccacct cttcgaagac tgaaaaagca aaatgagttg
     4321 ttactggcaa ttcaatcccc tcttccttat aaacacgaag aagattgagc aaaattgctg
     4381 cgctgacctt gtcatccaag tggcgagact tgataaaacc agtctctgtc acgacagttc
     4441 gtggatcgaa gctgataaaa tcaccgacct caatcccaag agcacgagtt tctttttcat
     4501 tgcttacttt tacatccaaa cgcacttcca tattgtcctg cgtgcgttct gcagttcctg
     4561 catccttgta gacatggcaa gaagtttggt ggatgaggat ggttcctgat accttttgac
     4621 ctgtgctagc cacatgaacg gtacagtttt caccttcaat catgttccaa ggaaaaccac
     4681 cgatacggtc caatttaaga cgaccatctg gtttgacagc acgaacaata gcacccagcg
     4741 tgtccacatg ggcagtcaca tagcgatgtt gctcatcatt ttgacctttg atggtcacat
     4801 tgacaccacc cttggctgtg cgaactggct ggtaaccaaa accttctaga gtcttgacta
     4861 aataattcga aatttcacga gtgaagcccg ttggcgatgc gatggctgtc agttctttga
     4921 tatattctac tgtttgattc atttcttcac ctctttcgct acctattata acacatttat
     4981 catcggataa aaaaagaaaa tcactcctgt agacttggct acaaaagtga tttaagtgat
     5041 tattccattt caaaaacatc gctttcttgc ttgtttggta gaaccaatga gagcaagatt
     5101 ccaacaatgg ctggcaccaa ccatgggaga gatgccttgg caaaaggaag aacgctaaca
     5161 agatttgcaa gaaactcaac cttaaacgag cttcctagta cgcttgcaat ggcaataact
     5221 gtaaccacag caattgtcaa ctgcatacct ggttttgaaa gggcaacaaa tttgttgaca
     5281 atgacaatca taacgatagc aatcgtgatt gggtacaaga taaccagtac tggaattgag
     5341 tacttgataa tcgcatcaag acccaaattg gcaatagcaa atccaatcaa ggtaaaggct
     5401 gtcgcataaa ccttgtagct gatttgtggg aagcgctcat taaagaactc agctgttgac
     5461 acaatcaaac caacagtcgt tgtgaagcag gttacggtaa ccatagctgc aaggaagagt
     5521 tgagctgttg agccaaagat ttcttgagtg gcttgtgaca agatgtaaac acctggtgtt
     5581 ccacccttca tcgcttcagc tggtactggg aaatgatttc caagaaaacc taaaccgatg
     5641 taaagagcgc tgaaggcaag ggcaacaacg ataccaacaa cccaaatagt tgaaatgtat
     5701 tctttcttac ttgaaaatcc aagttgtttc aaggtttgaa ctgcgattac gctaaaggcc
     5761 actgaggcaa gggcgtccaa ggtattgtaa ccttctagga aacctgtacc aaaggcagaa
     5821 gcttgataag cagctgaagc agcttgagga cttgttccac catatttgat agctcccaga
     5881 acgaccaaga taacaatcaa aattgcaaag actggcgtta aaatacgtcc aatgcggtct
     5941 aagatttttg atggattaag cgaaatcaaa taggctgccg caaaatacag aaccgtaaat
     6001 acaatcaagc caagtccttt atttgcatcc gacaaaaggg ggctaatccc tacttcgtaa
     6061 gctgttgtag cagtacgtgg ggtagcaaag aatggaccga ttgacaagta aagaactgag
     6121 aggtaaagag tcgcaaacca aggcgctatc ttcgttgaaa tctcgtagat atatccttta
     6181 ggatttagcg ttccaataat aagggtcaag acggcgatac caacgcctga aaagacaaaa
     6241 cctgcgatgg caggaagaaa atgttctcca gatagagcac ctagagaagg cggaaaaatc
     6301 aagttccccg caccaaaaaa tattccaaac aggagcaaac ctgttagggt acctttttta
     6361 gccataaaaa tctccttcat atttataaaa tactgaccta gtataccacg attaggagtc
     6421 tgaaaaaagc atcacgaggc aaatattaaa tgttttcaat tttcttaaaa ataagaatta
     6481 tctaaaaata aaactctcct acccagtcca caccaagtag gagaaatgca aaattttaaa
     6541 gttcttcaaa ggctaacatg ccaccctgga cattgataac attataacct tgttctaata
     6601 ggaattggca agcacgcgcc gatctcattc cagatttgca aataatataa tgcaagcgat
     6661 ctttgtccaa ttaatcataa ctatcagcca attgactaag cggtaggttg tgggcacctt
     6721 ctaaatgaag agctgcaaac tcatccactt ctctcacatc cactaaagaa agctggtcgt
     6781 tttggtaaag ctggtaaaat gcgtcaaagg ctatttcttt cattcttttt cttctaatac
     6841 tcttcgaaaa tctcttcaaa ccacgtcagc gtcgccttac cgtgcgtatg ttaccgactt
     6901 cgtcagtttt atctgcaacc tcaaagctgt actttgagca gcctacgact agcttcctag
     6961 tttgctcttt gattttcatt gactataaaa tggttttaat tctttttttc aaatctggca
     7021 ctacttctgc ctcaaaccaa ggatttttgg ccatccagat ttgatttcgt ggtgatgggt
     7081 gaactagcag aaaataggct ggcagatagt ctttatagtg tttcaccctc tccgttacct
     7141 tcccactgat tttctcctgt aaatagtagg cttgggcata ttgcccaatc aagagggtta
     7201 actgaatatc aggcaattcc tgtaagagct gcggatgcca tttttctgca aaacctgtac
     7261 gaggcggaag atcacccgac ttgccatgtc ctggaaagta gaaatccata ggcaaaacag
     7321 caaaataacc tgaattgtaa aaggtatctt catctacacc tagccagtcc cgcaagcggt
     7381 caccactttt atctttccag taaaggcctg cttcttgagt tttaagtccc ggagcctgac
     7441 cgatgatatt gatgcgagca gtttttggcg ctgcaaagag aggctcaatg ccacgctctg
     7501 tatagctggc attctgcgaa tccgccatga tagcctgttt tattctttcg atttgagaca
     7561 tctactttcc ctccaaaaag aagtccaagc ataagatctc agacttctat cgttttattt
     7621 gatcaattcg taaatagctt cggcataaat tgctgctgct cggaagagat cgtccaaggc
     7681 aataaattca ttggcttggt gcatagtatc aattgagtct gggaacatag caccataggc
     7741 aactccgcgc tctaacaagc gaccaaaggt tccaccaccg atgacttgct catgaccttg
     7801 taggccagtt tgtttttcat agacattcaa caaggtttgc acaagtggat cttccattgg
     7861 cacatagtga ggggtatgac catgttctga tagactgaca gaagcaactg gcaagttttc
     7921 taggattgac ttgatttgtt ctggacttgt tccttttgga tagcggatgt taagggcaat
     7981 ggtattatca gcacttgttt catcgaagcg gaagacgccg gcattcatag aaagagcccc
     8041 catcttttca tccacatgag caatcttgag attttcaccc tcatgatcgt tcaagagaat
     8101 tttacctgcg atgtcaaggt agtctttggc tggaccagca aagccaaact ggctgaggaa
     8161 gagggcaagg taagttgcgc cattgacacc tgaagcaggc atagcaccgt gggctgattt
     8221 accaatgatc gtcaccttgt atttgccagc ttcttcttgg agttctcctc taagtttgtg
     8281 ttctgcaaca aaggcatcta gtttagcttg caagtcagcc aaatcacctg aaacgactgc
     8341 tgttgctgat tctggtacca tattttcacg taaaccacct gtaaagctgt gaagacgggc
     8401 agcacctgta ttttctcctg caaagtggag gtattccgtg atatttcctt tttcaccatt
     8461 gatgattgga aattcagcat ctggtgagaa accgaaatct ggtttggcaa gtcctacgtg
     8521 ctcaaagtag tagtccatgt ctgcccagcc tgattcttcg tctgttccaa cgatgaagcg
     8581 aactttctta gaagttggaa ggcctaattc tttgatgatt ttcaaaccat agtagcaagc
     8641 tgttgtaggt cccttgtcat ctgaagcacc acgcgcataa aggcgaccat ctttgatagt
     8701 tggtgtgtaa ggatctgtat cccaaccgct accagctgga accacatcca tatgggcaaa
     8761 gattccgaga acttcttctc catcaccaaa ctcaaaatgt cctgcatagt tatcaacatt
     8821 tttagttgga taaccatcac ggtctgcgat ttcaaggaat ttttccaagg cttttactgg
     8881 accaggtcca aatggatgtt gggcatcagc cttgctatca tcacgttctg agttgatttc
     8941 caaaaggcta aacaagtcag ccaagaggtc ttctttgcgt ttttctactt ctgctgtaaa
     9001 atcaattgct atcatttttt cttcttccta tcttttttcg atgatttcat ctactggcaa
     9061 gcggtagctt ggttccaatt tgtcgtctgt gtaacccact gtgatcaaga gttctgggcg
     9121 gaaacggtct gcgatttcca aaacttcatt aacttttgat ttgtcaaaac caaggataat
     9181 attggaacca attccttggt ctgtaagagc gagaaccaag ttcatagcaa ccaaacctgc
     9241 attgagtgct aggtagtcgc tgacttgttg ttcattgtag cgtgcaaatt cagctggcaa
     9301 atttttcatg aagtattgaa gttgttcttc tgagaaattg ttagcaccac caactcgggc
     9361 aatcttacga gcgcgtttgg caaggtctgt atctgtaaat agggcaatgg ttacaggtgc
     9421 tgatgatacc tgttcaaaat tggaaccata agctaacttt gccagttcag catttttctc
     9481 acgtaccacc acaaatttcc aaggctggct gttgtgggcg cttggcgcca aggttgcaat
     9541 ttcgatagcc gtacgcacat ctttgggatc aacaggctta tcagtaaaat gcttggtcgc
     9601 atgacgtttt ttatttaatt caagaaattt cataatcgat ttccttttct aattctactg
     9661 cttccattct accataattt gaaagagctt gcagggattt ttcatgatta agtttttcta
     9721 tcacagacat aaaagatata tattggttta tcaggaaagt ttctgataaa ctagcaagta
     9781 ctttacattt gaatggagac agtatgaaaa aatttagcct attactagct atcctaccat
     9841 ttttggttgc ctgtgggaat caagctacac ccaaagagac tagcgctcaa aagacaatcg
     9901 tccttgctac agctggcgac gtgccaccat ttgactacga agacaagggc aatctgacag
     9961 gcttcgatat cgaagtttta aaggcagtag atgaaaaact cagcgactac gagattcaat
    10021 tccaaagaac cgcctgggag agcatcttcc caggacttga ttctggtcac tatcaggctg
    10081 cggccaataa cttgagttac acaaaagagc gtgctgaaaa atacctttac tcgcttccaa
    10141 tttccaacaa tcccctcgtc cttgtcagca acaagaaaaa tcctttgact tctcttgacc
    10201 agatcgctgg taaaacaaca caagaggata ccggaacttc taacgctcaa ttcatcaata
    10261 actggaatca gaaacacact gataatcccg ctacaattaa tttttctggt gaggatattg
    10321 gtaaacgaat cctagacctt gctaacggag agtttgattt cctagttttt gacaaggtat
    10381 ccgttcaaaa gattatcaag gaccgtggtt tagacctctc agtcgttgat ttaccttctg
    10441 cagatagccc cagcaattat atcattttct caagcgacca aaaagagttt aaagagcaat
    10501 ttgataaagc gctcaaagaa ctctatcaag acggaaccct tgaaaaactc agcaatacct
    10561 atctaggtgg ttcttacctc ccagatcaat ctcagttaca ataacactct tcgaaaatct
    10621 cttcaaacca cgtcagcttt atctgcaacc tcaaagctgt gctttgagca acctacggct
    10681 agcttcctag tttgctcttt gattttcatt gagtataaga tatactaaaa gcgatgggat
    10741 cagccaatcg cttttttagt ttgcattggt tattttagga aactcttaca aaaaatcaaa
    10801 aaaattcttc acatcctcta catacccatg cttttctatt aagcttgcta atagttccag
    10861 attgtgcccc tgatagttat cttcacgacg tagttcttca tacatttcga taaagggaag
    10921 ccccttggat ttggcaaatt ctaactcagc ctcgtaatca taagcgactt tacgaaatag
    10981 gatatttacc aattccccat cttcaacctc tatcacggca tactgggcac ggtgattttt
    11041 taacgcctcc caattaaaat agggcatgcc aatcgaccct ggattgatga tttgttgccc
    11101 ttgactacca taacgaagca actgcttgtg aacatgacca taaactgcca cgtccgtttc
    11161 cgcatctagc agttggtcaa atttctctgt atcattctca actagcaagt caccaccata
    11221 gtttttgtca ggtaaattat gagagataga aaagcgcaat ccgtcaattt ctttcttttc
    11281 cagcaaaggc aagcttcgta gccagacaat cgttgcaggc tccattcgct ccatcaaata
    11341 ctgtgtcata cgcaagagct gaacttcctg tgggtcttct aagccatatt gcccatctaa
    11401 agcctcaagg acacgatcat cccaattgcc tcgaacactt gctgtgatag gaaggtcctt
    11461 tagcagggcg actaagtcat ttgcgcctgg accaggaaga aaaatatctc ccagaagcca
    11521 gtattcactg actccttgat ttttagcatc tgcaatcact gcttctagcg ccgtcgcatt
    11581 gccatgaata tctgataaaa ttgcgatttt atggttcatt gtggtttcct tctgtttggt
    11641 aatctactct ttcttccgcc acttgaagca agatttctgt ttcctgcggg ttcaactttc
    11701 tttgcacagc ctctgctact gctcttggta ctccaacttc gacaatctca tccacaccgg
    11761 cttccttgat tttggtcaga gacttgaagt gcctcatgag attttgcttg cgtttaggtc
    11821 ccagaccatc aatcccatcc aattgagatg agaaagaatt tttggagcgc agttggcggt
    11881 ggaaggtaat ggcaaagcgg tgcacctcat cttggatgcg ttggaggagg aaaaattcct
    11941 gagaattgcg agacaagtcc accacctcaa gcggatctcc aaagagcagt tcatgggttt
    12001 ggtgcttatc attcttttgc agacctgcaa ttggaatatc caagcccagt tcttcttgga
    12061 tgacttgctt agcaatattg acttgacctt gccccccatc aatcacaatc aaatctggag
    12121 gagtcaaagc ctcacgctgt actcgaccat agcgtctgcg aatgacttct ctcatactag
    12181 catagtcgtc cggcccaaca acggttttta tcttgtactt acggtaatct tttttacttg
    12241 gcctgccgtt aacaaagacc accatggctg aaacaggact ggtccccatg atgttggagt
    12301 tatcgaagga ctcgatgcga actggagtcg gaatttggag caaacgtcct agattttcaa
    12361 tagctccttg ggtcttttcg actgattttt ctagcaggtt aaatttctgc tccagactaa
    12421 cacgtgcatt ttttatggct agattgacca gctgtttttt ctctccacgt tggggtttga
    12481 gaatcttggt atccaccaaa gccttgacag cttcttcgtc aatatcctgc ggaatcagta
    12541 cctcattggg aactaggtga gatttttctt gatagaattg tcccacatag gtcaagaaat
    12601 cctcatctgg atcattgaag taggggaaga gattgacatc gcgctcgatg agcttgccct
    12661 gacgaacaaa gaaaacctgc acacacatcc agcccttatc cacatagtag ccaaagacat
    12721 cgcgattttg gagatcctta gccatgaccc gttgcttggt tcgaagtgtt ccaatggcct
    12781 gaatcaggtc acggtattcc gctgcccgtt caaactccat actttgtgct gttactgcca
    12841 ttttactctt gagatcatcg atgattttgt catcctgacc tttcagaaaa tcagacacct
    12901 cctgggccat ggacttgaaa taggcctcgt cattcttaca gatggtgtgg gccatacatt
    12961 ggccgatatg gtagtaaaaa cagaccttag agggcgggtt ggtacatttt cgaaaaggga
    13021 aaatcctatc tagaagtcgc ttgatttcat tggctgcccc cacatctgga taagggccaa
    13081 aataaagacc accgtccttt ttgacctgac gagtaatgat aagccgtgga tagtgctcat
    13141 tggtgatttt gataaaagga taagacttgt catccttgag catgatattg tacttgggct
    13201 tattttcctt gatcaggttg atttccagca gaagcgcctc aatattagac tccgtaacaa
    13261 taaattcaaa atccacaatt tcagacacca aggcctctgt tttggtatca tgacttccac
    13321 ggaaataaga cctcacgcga ttacgcagat ttttagcctt tcctacatag ataatggtgc
    13381 cgtttttatc cttgtaaatg tagcaaccag ggctggtcgg caagagctct agttttggtt
    13441 taatcaagtt gttcatagtt ccattatagc aaaaaagtag ggaaagatag taccctactc
    13501 attagtctca tatatttttc ggaggtttgc aacatcctct tcctggatac cataaaagga
    13561 acctgctggt tgcatgacag acttctcatc tgtatggtcc aagccaatcg catgacctaa
    13621 ctcatgttct gccgtatgga caaggcgttc ataggaatag ccatagtatg gattggacaa
    13681 ataataatga ttcaaccgca ccgttacgga caagaattgc cctgttaaga gattagtttg
    13741 actttccgcc tctcctgcca caggagtgcc tccgtcgttc atctccgtag ccgtaatatc
    13801 cgccttacta gactcagtca cgagttcaaa gttaaaagca ccagtttgat tccagttctg
    13861 gattgcttct aaataagcct cttgaaagcg tgaatccatc tgcggatcca ggtaaatacg
    13921 agcagaggcc tgtgcccacc ttccttcaga atgctggtga ctatctgtct ggcttaactt
    13981 aggcagttgc cctgttttct cccattggtc aatactttgt tgaccaattt ttactgaccg
    14041 ttctgcttgc tttagcgctc cttgaaaatc cccgttcaga taccagagaa gtccgaaagc
    14101 aagcacacat aagagcacaa ctatccaaac cagacgccaa aacaaacgcc acacaaaaga
    14161 aaagaaagcc cctatcaaac gaaaaagcca gcgcatgtct ctccaccttt ctagcaaata
    14221 agaattattt tactaaaaga agctaaaaga aatctaaata ctgacttttt catcttaacg
    14281 tcctactatt tttttgagta actggatagc tttgtatttt aaaggcgatg gatggtaacg
    14341 gaaagtaccc gctttgcgta caatatagcc attaaaattc tgtttaaaac gcaaaacacc
    14401 atcacttcca tcaaaaatcc cttgaatgcc taggaaattg tatttaggta ttccacgttt
    14461 tatgctttcc aacataacat atttttgaag cagtgcaggg gcatagaact tattaaactc
    14521 agtgtaggaa ccactaaaga gataagtcgt ttcctgaggc atataaacaa ataaactccc
    14581 agctaaaaca atatcttctt ctccatattt ttcaatcaag tctcgcgctt ctgcttttcg
    14641 aacttcaaac gtttcaaatt gactagaata ttctctcagt tgattttgtt ttttctcaga
    14701 atgaggattt ttactcaaat caagtcgcaa cttgtccaag ttttcttcta gtttattttg
    14761 ttcaccttgc aatttgctca tatagtccga aaaatttaag cttgctatga gaaactccgc
    14821 ttgttctcca aaagtatcat aaaaatgctc ataatattct aaacttttat cactatattc
    14881 tctacgttca gaggtttctt ttgttatatt cttaaaaatc gatagttctt cacgttttaa
    14941 ctttttcaac cgaatgccaa aggtttcagc ctttttcacc aagggtttac cctttttgct
    15001 aaaactttta agcaaactct tttcagttaa ttcagttaaa tctttatagt ataaccaatc
    15061 tggttctcca cctgggtaac ctgttgttaa gccatcaaat tgataaccta aatcagtcaa
    15121 atcttgaata atactttttt tctcagcatc tattggatta ccttggctat caaaagtttg
    15181 ataagtttca tagggtttta caagcaactc taatacacca ttttgcttgg catattcttt
    15241 taactctgca taaaaaactg gaagagcatc ttgttgggta taaatcggcc ccgaattgag
    15301 ttccatatgc agaccaccca gcatgggcaa gctataaacc agagctgcaa cttgaatttc
    15361 tccttcttgt ttcaaagcaa gataaacaat tcgagcccct cttttttcta gcaaatcccc
    15421 catctggaca gattgcataa aggaacgaga agaaacctga tcagaataag tctgaaactc
    15481 ttctttcgtg agtgttgtta gtgccatata cttactttct atgttttttt cttaatgttt
    15541 tacggaaatc aagagcaagt cttaacagag gatagagagg atgagtgggc attgtaaatt
    15601 cacccaagta ttcttcaatc gttggattaa atttttcctt aaaatgataa agtccaccat
    15661 tgagagagtt ttcaacacca cctaaatttt gccagatcat acctcgctca aaggcatagc
    15721 gagccgtttc ataccatgtt aaaattggtg cattgtaacg tttaaaatca tcatccatac
    15781 cagcatatat attgacagag gtagtaccaa attccaaact caaagtagcc gctaaaggaa
    15841 ctctcgcttg acctacatct atatattcct gcaagaaggt caattcctct aacaaacgtt
    15901 ctttttcctt cttctgcgct tctacttttg aagttcgagt cgactcagta aacgtctctt
    15961 ccaaggctct atttttcgct aactgttctt ctaattcttg cgaacgttta gaaacatcca
    16021 aggtggccaa ggtgatatag gccttgtcct taaaattatc taacaatttt ttataatagg
    16081 cttcattcct caaatgaatc tcttttcgct tctcagtttt tttcatcaac tccgaaaatg
    16141 aatctaatag ttccagtcca ccatattgaa tctcaagccc tttgtttcgt gctgttcgaa
    16201 tagtctgttt tgttgacttg gaaagtttat cttcttcaaa attttccttg tatattttcg
    16261 cctgaatacg aggttgaatg gtgtctccca tttcctccgt ttttcctgac caccttactc
    16321 ccatttgttg caaactatca ataatagcca gattttcagg aaattctgtc ttttcctgat
    16381 tgattaaact ttgagatagg caaatacttg ggtcaaaagt cacaaaaacc gctctcttac
    16441 tgcgagcata ggacttaata gactgaatgg caaaattcaa gagttcttta tccccataat
    16501 ccaatatagg tcctcttggg atgtaaaaca ttttatagcc tagcggaaga gttctaatca
    16561 aaatactagc tgtcgccagt aatttttctt ccctgtaaac accaaacttc tcatgttgcc
    16621 aattagactt aacttcctcc caagcactac tttgtaatac attggctaat tcatgttctt
    16681 tgacaaactg atcatattct aatgtgggaa tgccaatttg ataacggtac atttcctact
    16741 ctctttcctc cagtatttta ttactattct actactttcg tttgaaaagt ctagtgaaaa
    16801 catattttat tttagttcta ctccccctat gctgaagttc aaactaagtc agtctctctt
    16861 ctaatttgaa atcaattggt agggttgtta aaaaaacttc caattgtttt tcccagtagt
    16921 accactcgtg agttccagcg ctatggctat aggtcacatc aaaacctagt tttttgagat
    16981 ttttcactgc gagattattg gcttcgtaca agaaatcctg ttcgccacac cacgcccaaa
    17041 gtttggtctt tttatccgat tttttagcca gactttcaag agaataggga ctagttgtcc
    17101 agtctctaat ctctccaaaa acacctctcc agtaggctgg acttcccaga ttttgacttt
    17161 caggagaaaa gttttgaaag ctgagggcac ctgaaaaact agctgcatga gaaaaacgat
    17221 ttgtcgcaag agccagtttg aaacagccgt agcctcccat agaaagacca gctataaagg
    17281 tcttttcacg cttgctagtc atattaggga agaagcgttt cagaacctgt ggcaattcct
    17341 ctgctagagc cgtgtagtag tcaaaaccat actgggtatc ggtgtaccaa ccattgctgg
    17401 tattgggcat aacaacgatg agattagttc ctcgaagcaa gcgttctaca ttggtccgct
    17461 taagccaact attatgattt ccagacatcc cgtgcaaaag gtacaagacg ggaatatctt
    17521 cacactctgg ttcttccact cgattggcat cagggtagag gacattcatc ccccactcca
    17581 tatccaatac ttgtgagtaa tactcgattt tcattactgc catgattttc tccttctatt
    17641 tttctataag aaaaagccga aactcgactt tctctctgtt tatttcaacg attattttgc
    17701 ttccatgact actggtaaaa tagctggacg acgcttggtt tggtcaaaga gatacttggt
    17761 caaattatct cgaaccttcc ctttaagatc tgcccagtca aagtcatctc cttgaagata
    17821 atcttctacc gtttggttaa tcaattctga actttcacgg agaatatcgc gactcttctt
    17881 gagataaaca aatccacgcg tgtgaacacg agctctagcc acaattttct tctcacgacg
    17941 gttgactgta atagccacga tgaaaattcc atcctctgac aagaccttac ggtcacgaag
    18001 aacaacattt ccaacatcac caatggcatt cccatcaatt aagatatctc ctgctgaaac
    18061 cgatccagct ggaacaaagt ctccattctc gtaagccatg gtcgtcccct ttttaggaat
    18121 gaagatgcgt tctggcaaca tcccaactgc catggcagcc ttagcgtgag catccaactc
    18181 acgatactcc ccttggacag ggaagaggta cttaggttgc aaaagattga tcatcagctg
    18241 caaatcacgc acatttccgt gccctgatac atgtaaactt tgggtaatca atttgacaac
    18301 cccacctgcc tgataaatca tattttctac acgcgcaaca aaggcttctt tagcaataga
    18361 cggagccgta gcaatataga ccaggtcccc atccttgatt tctacataac gatggcgacc
    18421 aatcgacatc ttacgaagtc cattgatagg ttcacccata cgacctgtct caagaataat
    18481 caactcatgg tcttcaaagc gagacatatc tttaggctta atcaagagaa tttcgttggc
    18541 taaagacaac ttcttaagac gaatcgctgt gcggacgata ttttcaatat caaatcctgt
    18601 caagacgata cgtcgacctg ttttatccgc agcgtcaaaa atctgctgaa tacgagaaag
    18661 attactggaa acagctgcaa cgatgatacg accttcccag tcagcaatag tttgggtaat
    18721 ttcatcccta acttcacttt cactagccac ctgaatattg ctgtctgcat tggccgaatc
    18781 actgaggaga gccaggacgc cgtcacgacc aatctctgcc aaacgagcaa aatcagttgc
    18841 ataagattca ctagccgttt ggtcaaattt gaagtcacct gtataaacga tgcttccttc
    18901 cgatgtcttc aagacaattc ccagactctc tggaacggag taagtcgtag ggaagaagga
    18961 aaccactgtc ccaccaaaat caatctccgt attctcatca atgacatgga aatcattaaa
    19021 tttcttaacg gcatcatttc ctttgacaaa gagctttgcc aactcaatgg tcaactcaga
    19081 cccaaataca ggaactttag cctctgccaa gagataaggt agagcaccaa tcgcatccgc
    19141 atgcccgtgg gtcaagaaaa ccccagcaat acggtcgcta ttttcaaaaa ggtaatccat
    19201 gtttggaatc accacatcga cccctaattg ttcattttca ggatatttta accctacatt
    19261 caaaacaaaa atggactctc caatttcagc aatgtacata ttttttccat tctcacgcac
    19321 accaccaagt gttgttaaac tgatattact catttttcct ccaaaatctt agtttatctt
    19381 gattataggg caactctacc ctcttattct aaaagttcta atactttcag ccgaatcttt
    19441 tcctaccatt ataccatttt tttgatgatt ttcaaaatga aggtctgttt tcaaaaaaga
    19501 gttgatttcc caactctttc ctaacttcta ctctttttcc atcaaaaaat cataaatttc
    19561 ttgcaccgta cccagcgcca tcatttcttg gtctttaacg gtttgtttga gattttggta
    19621 aatttgactt tctcctatct cccgctttgg cgcctcttta ttgactgtca tcacaccatc
    19681 ttttatcgtc acaaagccta gttcttcaaa tacttgaatc atcttgacca gcaagatttg
    19741 ttgaatatta agatatgcag ccaaatcttt cagcttgtag cgaatatcaa actctgggaa
    19801 ctggtaaata gtcttgtaca atttggcaaa ctgatctcta gtcccataac ctgtcagata
    19861 ataggccttg tcaatatcat ttttgaaata gacagcagag aaatgctgtt cctgaaaaat
    19921 ggtcttcagc tgagtaatat cctctggaat gttttttacg acaatagctt cactagccgc
    19981 aagatttggc agttctccag gaaaatccaa gactggaaca ccttctggca agactgcatt
    20041 ttttccacga atgttaaaaa gttgaacacc ttccactcgc gcatccacca tcatcaactg
    20101 gagggcagtt tggccattcc attggttgac agacaattta accgctaact ctagattctt
    20161 ggtttgagaa aactctgtcg cccatctgcc ttgaccaaag gctaccactt caaaactcgc
    20221 ctcacccttg gaaattttca gctttagatg ggcattacct gcccccatag tacgagcact
    20281 ttcgacctga aaattcttga tataaaaaat aggtttctga ttatccattc caaaaggagc
    20341 taaacgttca aaacttttga ccgtttccaa gctaagtgcc tccaaatcca actcttcatc
    20401 taggtttaac ttattcttgc caccagcatc tgcacctttt tcacgaacat aatcttccaa
    20461 aacctgagat aaatctgaga gttgctcaac ttccagcgtc atacccgctg cacctgcatg
    20521 acctccaaag gcgataaaga ggtctcgatg gggatccaga gcttcaaaaa tatcgaccgc
    20581 ttccacacta cgagcactgc ccttggcacg accgtcttct atattaagaa caatgactgt
    20641 ctgtcccaat tcttccaata aacgaccagc cacgattcct agaaccccag gattccagcc
    20701 ttccttggcc aagacctgaa ccttcttctc aggatccacc atggtcttgg cttcttcata
    20761 gatagactga acgatttcct tgcgctcttc gtttttctgg tgaatcataa aggcaatctc
    20821 atgcgcttcc tcatcatcaa atccagtcaa caaatcaatg gcaggattgg gatcatccaa
    20881 gcgacccaag gcattcaaac gaggagcaat ctggaaacca accgtttctt ctgttacttc
    20941 gttggcagca atcccagcca tgtccagcat ttcttgcaga ccaatgcgct gggtatgacc
    21001 caacatttcc agaccatatt gaactaagat acgattttca tccgtcagac tcaccatatc
    21061 tgcaatagtt ccaatagcga ccaaatcaag caattccact tgcacttctt ctaacagggc
    21121 acaagccaac ttgaaagcaa ctccacaacc agccaaatat ttaaaaggat aatccgcatc
    21181 tggatgttca ggatggacaa tagcataagc atctggcagg gtttcaggca tggaatgatg
    21241 gtctgtcaca atgacatcta ctcccataga ctgagccaat gcaatagcct catgaccagc
    21301 aaccccattg tccaccgtca caatcaagga aattccttct tgctcgataa agtatttata
    21361 aacactagca ttagggccat agccatcggt aaaacgattt ggcaggtaaa ctcggcactc
    21421 agcaccaagt tgttccaaac tttccttcac aatagaagcc gaagtcatgc catccgcatc
    21481 atagtctcca taaacgagaa tattttcccc ttcttcaata gcctgacgaa tccgctccac
    21541 tgccttgtcc atatcatgga gcagataagc atcatgtaag tcctccaagg aaggttctaa
    21601 aaacttcttc agactttctt ggtcctgaat ccctctctca aacaataacc gagccacctc
    21661 aggacccagt ccagccttct tggctatctt tgtaaaatcc gcatcttcta cctgcagggc
    21721 aaactgccat tcataagtag gtgttatcaa aaagacatcc actctttcta agaattctat
    21781 tgcttcatta taacatgatt tagacgtttt ctctatccag attgcttttt tataacattt
    21841 tatagtatat tgaaactaga atagtacacc tctacttcta aaatattgtt agaaatcgat
    21901 ttgactgtcc cgatcgattt gtcctgttct tatttcacta tagtgaattt tttcagatat
    21961 ggtttgacaa gaacattctt ttattgtaga atcgtgttat aaaatctaac acaaaggaga
    22021 tctctcatgg ctttagtaga atttaaaaac gtcgaaaaat attacggaga ctaccacgca
    22081 ctccgcaaca tcaatctccg ttttgaaaaa ggacaagttg ttgtcctgct tggaccttct
    22141 ggctctggga agtccactct tatccgtacg atcaatggtt tagagactgt tgacaaagga
    22201 agtctcctag tcaatgggca ccaagttgct ggtgccagcc agaaagattt ggtacctctt
    22261 cgcaaggaag tcggcatggt ttttcaacat tttaaccttt atccacacaa aactgtgtta
    22321 gaaaacgtaa cacttgcacc cattaaagtt ctaggaattg ataaaaaaga agctgaaaaa
    22381 accgcccaaa aatatctgga atttgtaaat atgtgggaca agaaagattc ctatcccgcc
    22441 atgctatctg gtggacaaaa acagcggatc gccatcgctc gtggtcttgc tatgcatccg
    22501 gaactcctcc tctttgatga accaacatct gctcttgatc ctgagactat cggagatgtt
    22561 ctagcagtta tgcagaaact ggcgcatgat gggatgaaca tgatcatcgt tacccacgaa
    22621 atgggctttg ctcgagaggt tgcggaccgc attatcttta tggccgacgg agaagtttta
    22681 gtagatacga cagatgtcga taactttttt gacaatccaa gcgaacctcg tgcccaacaa
    22741 ttcctcagca aaattatcaa ccacgaaagt gacaaagtca aataaggagg cgcctatgaa
    22801 aaaaaaattc tttttatcag cattattgat tagccttttc ggccttgctg ctgccaaacc
    22861 agtccaggct gatactagta tcgcagacat tcaaaaaaga ggcgaactgg ttgtcggtgt
    22921 caaacaagac gttcccaatt ttggttacaa agatcccaag accggtactt attctggtat
    22981 cgaaaccgac ttggccaaga tggtagctga tgaactcaag gtcaagattc gctatgtgcc
    23041 ggttacagca caaacccgcg gcccccttct agacaatgaa caggtcgata tggatatcgc
    23101 gacctttacc atcacggacg aacgcaaaaa actctacaac tttaccagtc cctactacac
    23161 agacgcttct ggatttttgg tcaataaatc tgccaaaatc aaaaagattg aggacctaaa
    23221 cggcaaaacc atcggagtcg cccaaggttc tatcacccaa cgcctaatta ctgaactggg
    23281 taaaaagaaa ggtctgaagt ttaaattcgt cgaacttggt tcctatccag aattgattac
    23341 ttccctgcac gctcatcgta tcgatgcctt ttccgttgac cgctctattc tatctggcta
    23401 cactaataaa cggacagcac tactagatga tagtttcaag ccatctgact acggtattgt
    23461 taccaagaaa tcaaatacag agctcaacga ctatcttgat aacttggtta ctaaatggag
    23521 caaggatggt agtttgcaga aactttatga ccgttacaag ctcaaaccat ctagtcatac
    23581 cgcagattaa ggaggatacc ctatgacaga tttatcatct tggacagcct attttcagga
    23641 ttttggacaa tttttcaatg gtttcctctt tactcttgcc ctagcgattg gatcctttat
    23701 cctcgccatg gtcttaggaa tcttctttgg tgccatgtca accagtaaac gtccaatttt
    23761 acgcatttta gctcgcatct ttgttgaatt ttaccaaaat actcccctct tggtgcagtt
    23821 tgttattgtt ttttatggcc tacctcttat cagtgaccat atcatcatga ttccaattta
    23881 ttggacagcc gttctctgcg tgggactcta tcacggcgct tatatcgctg aggttattcg
    23941 ttcagggatt cagtctattc ctagtggtca gatggaggcc gccttgtcgc aaggttttac
    24001 ctatatcagt gccatgcgct tgattatctt gcctcaggcc ttccgcatca ttctccctcc
    24061 attgaccaac caaattgtta acctcatcaa gaacacctct actgtagcta tcatctctgg
    24121 agtagacttg atgtttgtga ctaagtcttg gtcggctctc aacggaaact atatcccagc
    24181 ctttttaggt gctgctcttc tctactttgc cctatgcttc cctgttgccc agtttggtcg
    24241 caagatggag caagccaaca aaaaagccta ttcactttag gaggttacta tggaatctat
    24301 tttagaagtt ttaaccccag ataacctagt ctttatcttt aaaggatttg gcttgaccct
    24361 ctatatttct ctgattgcca tcatcctctc tactatcatc ggtacggtgc tagctgtcat
    24421 gagaaatggc aaaaatcctg tcttgcgaat catctccagt atttatatcg agtttgtgcg
    24481 caacgttccc aaccttctct ggatttttac tatctttttg gtgttcaaaa tgaaatccac
    24541 accagcaggt attacagcct ttactctctt tacatcagca gccttggttg agattattcg
    24601 aggcggtctc aatgccgtag acaagggaca atacgaagca ggaatgtcac aaggcttcac
    24661 ctcagcccaa atcctctact acatcattct cccacaagcc atccgcaaaa tgctaccagc
    24721 catcatttct cagtttgtta ccgtgattaa ggataccagt ctcctctact ctgttatcgc
    24781 cctacaagaa ctctttggag ccagccaaat tctcatgggc cgttattttg aaccagagca
    24841 ggtcttcagt ctttacatcc tgattgccct catctacttc agctttaacc tagcaatttc
    24901 tagcctgtct catatgctag ccaaacgttg gcaacaagct gcagaataat actcttcgaa
    24961 aatctcttca aaccacgtca gctctatctg caacctcaaa acagtgtttt gagcaacttg
    25021 cggctagctt cctagtttgc tctttgattt tcattgagta taaacagcca ctctccagtt
    25081 caattggaga gttttttgat gagagtagat atttatagac ctcaatttga caccatacat
    25141 tttctatgat aaaatgtaac aaattcattt acaacaaatc gaaatctaaa tgaaatggaa
    25201 ttttctatga aatcagtcaa aggactactc tttatcatag ctagttttat cttgactctt
    25261 ttgacttgga tgaacacttc tccccaattc atgattccag gactagcttt aacaagccta
    25321 tctctgactt ttatcctagc cactcgtctc ccactactag aaagctggtt tcacggtttg
    25381 gagaaggtct acaccgtcca caaattcaca gcctttctct ccatcatcct actaatcttt
    25441 cataacttta gtatgggcgg tttgtggggc tctcgcttag ctgctcagtt tggcaatctt
    25501 gccatctata tctttgccag catcatcctt gtcgcctatt taggcaaata catccaatac
    25561 gaagcttggc gatggattca ccgcctggtt tacctagcct atattttagg actctttcac
    25621 atctacatga taatgggcaa tcgtctcctt acatttaatc ttctaagttt tcttgttggt
    25681 agctatgccc ttttaggctt actagctggt ttttatatca tttttctata tcaaaagatt
    25741 tccttcccct atctaggaaa aattacccat ctcaaacgct taaatcacga tactagagaa
    25801 attcaaatcc atcttagcag acctttcaac tatcaatcag gacaatttgc ctttctaaag
    25861 attttccaag aaggctttga aagtgctccg catccctttt ctatctcagg aggtcatggt
    25921 caaactcttt actttactat taaaaattca ggcgaccata ccaagaatat ctatgataat
    25981 cttcaagccg gcagcaaagt aaccctagac agagcttacg gacacatgat catagaagaa
    26041 ggacgagaaa atcaggtttg gattgctgga ggtattggga tcaccccctt catctcttac
    26101 atccgtgaac atcctatttt agataaacag gttcacttct actatagctt ccgtggagat
    26161 gaaaatgcag tctacctaga tttactccgt aactatgctc agaaaaatcc taattttgaa
    26221 ctccatctaa tcgacagtac gaaagacggc tatcttaatt ttgaacaaaa agaagtgccc
    26281 gaacatgcaa ccgtctatat gtgtggtcct atttctatga tgaaggcact tgccaaacag
    26341 attaagaaac aaaatccaaa aacagagctt atttacgaag gattcaagtt caaataagta
    26401 atcatactct cctgggattt cccaggagag ttttttatag ccaaatgaaa taagacagga
    26461 acgatggaat tttggcattc aaatcaattg ataagaatgt tttatagtgg agtgaaatag
    26521 gatacgaaca aattgattag gaaaatcaaa tgaatttata gaaatctttt agcagttatg
    26581 ttgtcctatt ctagtttcaa aacgctatag aagcagcatt gtgctagtct agattcagtt
    26641 tactatacta aaacgagtag cttgaaatca aaaaacccac cctcacaggc aggttttatc
    26701 tgtattattc agctagatta tgctttacct tctgaaccga atacgtcgat acgttcttca
    26761 accgatgctt ggatagcttt tacaccgtca gccaagaatt tacgtgggtc gaagagtttt
    26821 ttcttgtcgt attctgcttc gtttgcttcg tagtcacgag caaatttacg agttgcgtta
    26881 gcgaatgcga tttggcattc tgtgttaacg ttaactttgg caacaccaag tttgatagct
    26941 gcttggattt gctcatcagg aatacctgat ccaccgtgca atacgattgg gaatcctgga
    27001 agagcttctg tcaatttttg caagtggtca aggtcaagac cttcccagtt tactgggtaa
    27061 ggaccgtgga tgttaccgat accagctgcc aagaagtcga taccagtttc aaccattgct
    27121 ttagcgtctt cgattggagc caattcacct ttaccgatga ttccgtcttc ttcaccaccg
    27181 atagtaccaa cttcagcttc tactgagata cctttagcgt gtgctttttc aacaacttct
    27241 ttagccaatt taaggttttc ttcaactgga aggtgtgaac cgtcaaacat gattgaagta
    27301 taaccaactt cgatacactc aagtgcatct tcgtagtgac cgtggtcaag gtggatagct
    27361 actggtacag tgatacccat tgattcaaca aggttagcga tcaagttgcg agcaactttg
    27421 taaccaccca tgtatttagc agcacccatt gaagtttgga tcaaaactgg agctttttta
    27481 gcttctgctg cgcgcaagat agcttgagtc cactcaaggt tgtttgtgtt aaatccacca
    27541 actgcataac cgttgtcacg ggctgcttgg acaaattttt ctgctgaaac gattgccatt
    27601 ttatcaggcc tcctgtatat ttttatgggt catcccattt acattgttca ttttatcact
    27661 ttttgccaaa aaaatctagt ttttcccgca gtttcgattg attttcttct aactccatct
    27721 atgtaaaccc tttctctccc tagtcttgga cgacttttgg aaaatctata aagaaggtta
    27781 aacgattctc ctccatctcg aaacgataag ctaatttttc atgttctaat agactcttaa
    27841 ccacaaagag ccccatacca gaccccttga ccttgcgact ggcattgtca gaaaaagact
    27901 gggctagttt ttcttgttcc tctgagctac agctattttc gataaaaagt tctccttctc
    27961 tttctccaat tcgaactaag ccacctggaa cagagtgctt aatggcattg ctgatgagat
    28021 tagaaagaat caacttcata actgatgggt ttagataagc ctgctgatgg gtcaaactat
    28081 tgtctatctg gagctctctt tccttggcta gcaaggcata atctttgacc agattttgcg
    28141 tcatctggag gaggtcaatt gtttccctat catctcgcaa ttcctgcaca gaagagaggg
    28201 aaagtatctg cagaacatgg tgattgagtt catccacaat ccccaaggca actcccagat
    28261 actggtctct atccttataa cgaccgatat tctctctcat attttcgatt aggattttca
    28321 aactagccag cggtgttttc aattcatgag aagctcctcg taggaattcg accttcatct
    28381 tctccagctg gagaatggct tcattctttt catgcaagtc cgcaataaca gtcaagagat
    28441 gctggtagag gctattgatt tgttccttga gattacctat ctcatcctta gaatccacgc
    28501 gcaatcgcac ttgggcatcc aggtacatca tccgacgagt cacccgcttg atttccaaaa
    28561 tcggtgcaac aatagtccga gcgtagatgt aggccaccaa gagggaaatc agaaaggagg
    28621 ccagcaaggt atagggaaga aactggagac tgatttgctc cgcttccttt tgtaaatcca
    28681 tggaagctag aaactggaga atcatagtac caccgtcttg cgttttcacc tcgcgctcct
    28741 caataaagag agaggttgtc tggcggtctg tgtccagagg aagactgtcc ttgacttcta
    28801 acttgtcctc ggtcatctca cctttgacgg tccccttgat atcactagtc tgggaataca
    28861 agtctaacac ttgctcgata ctctgcctat ctttcccttc tagggactgg gcaatggctg
    28921 ttgccttttg accaatggtt tcctgacgat gactcagata agtcgaagga aaaagaaaat
    28981 aaatagctaa atgaaggcag ataaccagaa cactaaatat cgagaaggta tagataaata
    29041 tctttgcaaa taaacctgtt cgtttcattt tcgctccaat ttataaccaa cattgcgcac
    29101 agtgaggata caatccaagt ctagcttttt ccgcaattcc ttgatataaa catcaataac
    29161 acggtcaaag ggaacctcat ctgtcgcttt ccagacggca tcgataatct gagatcgagt
    29221 caaggcccgg ccttcatttt tcactagata gtccagaatt tccaactctt tggcattgat
    29281 aggcacttct tgacctgcga tgcttgcact gtagctttca aagtccacct tggtatcctt
    29341 gtaagaaaag attcgtcctg tatcgtagta gcgcttgaaa atcgcgtcca ccctcacttt
    29401 taaaagggag agggagaaag gtttttccag atagccatct gccaaagagg caaaggcact
    29461 catcttgtat tcctcatcct gaaaagctgt caacatcaag acaggaacct gactggtttt
    29521 acgaatctca gctaggactt ctaagccgtt gagcttgggc atctggatat ccagtaaaac
    29581 cagggccacc tcatagctag aaaattgctc cagagcttcc tgaccgtccg ctgcctcaat
    29641 agtttcatag ccacaatccg tcaaataatc actgaccccc tcacggatca tctcttcatc
    29701 ttctacaatt aaaattttca tactttaact gctctctatt ttttattttt cttagaataa
    29761 atacctactc tattttctat tatagtctct tgctggcctt ttgtctgtaa gtaactgacc
    29821 actagataaa acgttgtgaa attcctttct cataaattcc ataactttag tatattatat
    29881 ttaagcacta aagtacaaag aaagcaactg aaagcaatga ttttcaccac tgctttcaga
    29941 tttattttga attgttaaat agccattcct atccactatt cttgaataga aacacaagat
    30001 gcaatcttta ttccagactc attttttcaa atttattcac catccagcaa gagttctttt
    30061 ggttgttttc taaggagatt gcttgaagca agcgccataa cgagaaccac tagaaccaag
    30121 gcaaggacaa aaatgatgat aaagtctgat gtctgaatgg aaatgtctag gctcgacaag
    30181 gtcttgctaa agccatctac ttctgcacca ccaccaaggt tagaggcttg agccgcctta
    30241 ctagcctgtt tggcaacacc tgaagtcaca ttggcaagga cagtgtttcc aattgcacgg
    30301 gcagtgtaat tagctaggaa gtaagcagaa actagagcag ggatagcaat caagatagat
    30361 tcggtgatga attgacccaa gatacttgcc tgcttgaggc cgatagagag gagaattccc
    30421 acttccttgc gacgggcgtt gatccaaagg ctgagcaaga gggcaaggag gagaactgag
    30481 aagctcaagc taccccagaa gaggaggttg gccatcttgt acataccaga gatagattgc
    30541 tcaagagctg ggtagttaga ggagctcttg acgagtgtgt agctcttcca gttgatacca
    30601 ctgatgccat tcaactcttt cataacatca tccaagttct tgtctgctgt tacaaagaag
    30661 gttgcgtccc cataaatggc tgtgtcttct gtgtatccat aaagttttgc agcagtgtga
    30721 atgtctgtaa tagctgtgtt ttcgtaaagt tcttgtgagt aggttactgc tgacttatta
    30781 tgaccatcaa agagtccctt gattgtcact tcaactgttt ccttggctcc tttttcatta
    30841 tctgcatcgt agatattaga gtccagttta accttgtccc ctactttcca gccgtgtttg
    30901 gctgccaagt ccttgtgcaa gaggatttta tccttgtcgt cgttggttaa gtgctctcct
    30961 tcgactagtt tataagaacc agagacaaac ttgtcttctt tagaggagtc attgacacct
    31021 gtaatcatca agctacttcc aaaacgtttg gcacgatcag cagtgagatt cttcttggtt
    31081 tctggcgttt caatcaggtc atatccagtc aaatctccga tagcgttgat acgtttgaca
    31141 taagactcga tggccttgtt ttcggtgatt tttttgatgt cttcaccctt gatattccca
    31201 gcaccacgag gcgttccttg gttgacgcga cgattgattt gcatggagaa gctattggtg
    31261 atatttttaa aggtctcctg agaagccttg gcagtagctc ccttgattga caagccgacc
    31321 aaactcaagc tcgccatgag gagaataatc aggaagatga caatcgattt gaaaaacttc
    31381 cttgtaacat aggcaaatgc gttgtgtaac atagattccc tttctagatt ttgttttaat
    31441 cattctatca aaataagctc aaattattta ctagtattgc gcgtttcagt cagtttctta
    31501 tcctttaatt caagtgtaat atctgacgct tgtgccactt ctttactgtg agttacgaca
    31561 atcacacatt tacctgtttt ctgggcaagt gatttgagta gttcgacaat atctccagca
    31621 gttttaggat ccagatttcc tgttggctca tcagctagaa taactggagc ttctgagacc
    31681 aaactgcgag caatggcaac acgttgctgt tgaccacctg ataactggag aacattccgc
    31741 ttgatctggc tttcatccaa accaagctca agaagtgtat tcttgcttgc ctttttgttg
    31801 accaatcgga tattttccag cggagaaaga taatctatca agttataatt ttgaaagacc
    31861 agggaaatat ggtgcatgcg atggtaagaa tagcccttct tacgaatatc ctctccttga
    31921 aaaaggatag aaccttcaac aggactatct agaccagcaa gtagggacaa gagtgtggat
    31981 tttcctgctc ctgactcccc aataatactg taaaattttc caggttcaaa attataatcg
    32041 atctgatata ggactgcttc agcagtattc ttataacggt aggtaacatc ttgtaattgt
    32101 aataaagtca tgatttctcc ttcttaacta atagatgata aaatttcttt cggtgatttt
    32161 ctaaataaga ataggaaaca aagggctaca gataagcaac taagcagaac tagaaaaaca
    32221 taggattctg caaaagataa gatgctagtt gataaactgc ttgctttggc tagtgtatct
    32281 tgtaagcttg cctgatctcc acttgctagt agagtttgga gtaggtaagt tgtgattccg
    32341 tttcctgcaa caaatgctgg aagcaaagct ccaagagata ccaaaactac ctctaaacag
    32401 aattgtagga agatcgagct cttgcctttt ccaagtgcaa gtaaaatccc cacttcatag
    32461 acccgttctc tcaaccagag agacaaaacc agaattaagg ctccagctcc tgctatcaac
    32521 atcccataaa ggaagatggt caggaaggtt tggaaagttg caactgagtc tttgatttgt
    32581 tcaaaagcct tgttttcctt ttcgacttgg tagccttgat tttccaaggc caagttttct
    32641 acctgcttca tgagtccgtc catttcctta ggattttcta catagaagcg tgctgcactg
    32701 acttgagctt cactattgcc caaaagggtt tggctacttt catagtctgt aaagacttga
    32761 ttttcactga agtcagaaga caagcctgtg aatttctctt gttttttacc agaaaagatg
    32821 ccaataatct caaactctac tgtttgtcct tttccagatt cagactgacc agcatccaag
    32881 ccaatcttgt catgaagtga aagaccgttc ttcttagcca attcttcgtg gataaggatt
    32941 ttcttggaat cccctttttg aaggtgtcgc ccttctttta gattgaaagc cgaactggta
    33001 aaggttacat ccttggatga atcctcaaga gccgttaagc taaccaagtt attgtctgca
    33061 gctgataaat catcacgctc cacgctctgc tctccagtca ctgcttcctt gtcttttagt
    33121 tttgcgaccg tctcaagttc aggagagaca ttttccagcc ccttaatctt gcttacagat
    33181 gctaggtctg acaacttgaa tgtctgacca ttctctatct tcttaataga aaaagatgta
    33241 ttgagtgatt tataaagatt gctttctact gttttgttgg acttcatcag agtcaaacag
    33301 gctgaaattc cggccaataa gaccaataaa atcagaaata aaataaaact tctcagtcgc
    33361 tttctgctga cataagccca agatctttgg attggattca tttgtcacct ccatatttgt
    33421 aagactatta taaaacccaa atatgaaatg tttatgaaat acgaaaaaaa aatatcgagt
    33481 aggggataat ctctagcccc tctcacacca ccgtacgtgc cgttcggcat acggctgttc
    33541 aactaacttt taacgcatgt cgttcaaggt aataatccaa acacgaaacc agtccacgtt
    33601 tttcaaggac tggttttgat atagcacgtt taagtaccga cttctgagct actatagtag
    33661 attgaaacta gaataatata cctctacttc taaaatattg ttagaaatcg atttgacttc
    33721 cctgatcgat tcgtcctatt cttatttcat tttaatataa ttgatagtgg tcgccccagc
    33781 cagatacctt atctgctatc catttaggaa cccctaactt aagcaatccc cataatcgtc
    33841 tcgatttctt cttccattgc ttccagataa tcactcgtag gcgagtacgc aagcgctcat
    33901 ctatgctagt gactatactt ttcatattta taattcattc ctttcgtttc actcaaggca
    33961 caacacagaa tgaaaaagtg ttgtgatctt tattttgttt tataataata gtgagaaagc
    34021 ctatcactac tacaaatcac ggggaggtga ataagtgagt ggtacagcca ctacctcgca
    34081 tattttgtca catcatttaa cggtgcataa taagttgtac catctgaata agttgctaca
    34141 atatcatttg catgctctcc ttcaccttta gcaaaggttg gagctcctgc tggatgattt
    34201 ttatttgcct ctttcaattt ttcaataatg gcattttttt ctgtatcttt tatattatca
    34261 ggatttttca ctaagatttt gtctggatat gtcggtttag cagaaacaat ttttactgtt
    34321 atttcttttt tattcgaagc acttgtccag tttccagcat tatctttagc atttaatttt
    34381 acagtaattc ctgaactagg aacttcagta gcaggttgat tatcaacatt attcaacttt
    34441 aatttcaaaa gagctgttgc atcagacgtt ttatcaatcg ttgtatataa tgatgaattg
    34501 ttattataac cagtttcttc atatttagct gtttgtgagc tacttgaaac agaactgaaa
    34561 ttatgcccac tacctccctg attatcttca atgcttacgt ctaaatgaac ttccccacta
    34621 ttatttggct tagcaacaac tgttatagta aaataacata aaatttgcat aaatagatta
    34681 gggaaatcaa agcaacttct aggaatgttt tatactcttc gaaaatctct tcaaaccacg
    34741 tcagcttcac cttgacgtac tcaagtacag cctgcggcta gcttcctagt ttgtactttg
    34801 attttcattg agtattatca gtcacagtgt actttcccag catcaagcca ctataactct
    34861 gcacataaaa atggagaaga tggcaatcct cttctccaaa tattaacttc tttacaaacc
    34921 aactatagtt gacaaagaac ctaaaatcaa ttgataacac aaggtcaggt cggtcaactc
    34981 tttcaactga agccctgtca actcttccca tttatcaatc ttgtattgga gagaattgcg
    35041 gtgcagatag agttgctggg ctgttttagt gagaacagca ctattttccc aaagagagag
    35101 aatgatttcc tgaatctgat cttgatccaa aatcatctgg tgtagacatt ctttaattgg
    35161 cttcaagtcc acgagtcttt ctcccatact ccaaagatag agctgagaaa aagtatgaac
    35221 accttggtga ccctgacgcc accatgtctt gaacaaatcc cgctcagctt tgattaagtc
    35281 tgatagggct tgatgtcctg tctgagacca aacctgaccc aacatgatag aaagacgaag
    35341 tccaaaatca tactcaaccg cttcaatcgt atcacttaaa atatctctta cagaagtgta
    35401 tttgtcttgt tgaagcacga aaacataatc ctgagctccg acctgtagca ctgtctgaca
    35461 attcggaaaa agagtccgca tcatatctag ccaagaagtc agattttcct gctgaaaata
    35521 agaaagatgg caataaacca actgaatctt tttaaaaact tgcggtgcct gtcccttgcc
    35581 ttcaaccaga taggaatacc aagggtttag cgaacgagcc tgctcctgct gggtcaaaag
    35641 ggcaaccaac tgcttttcac gctcgctgag cccagcttcc tccagcaaaa tccactgctg
    35701 agaagctaaa gggagcgtga gatagccctc tttctctact ggttggtctg aaatccgagc
    35761 ctcaggaaac cagtcttgta gttctttttc cctcatgttc tagccctcca ctttttggat
    35821 gcaccatgaa accaaactct caagacgttc cagattctca gtcatatgga gatagcccat
    35881 aaccgcttca aatcccgtgg acatacgata agtcacgaca tctgcatttt tagcctttgt
    35941 gtggctattg gtattgcggc cacgtttgta gatttcttct tctttttccg ttaggacctg
    36001 ctcctccaac atgagagcaa tcaggcgagc ctgagccttg gctgacacat acttggttgc
    36061 ttcttgatgg agtttattgg gtttggtcat acctttgagg atgaggtggc ggcgaatata
    36121 catagaatac accgcatctc cctcaaaggc tagcgcaatc ccgttaatga gattgacatc
    36181 aatcacgtgt ccacctcact ccatcttttg tatccaatag tttaatgcct tgagcagcca
    36241 gttggtcacg gatttggtct gctgtctcaa agtctcgatt ggcacgcgcc tcttggcgtt
    36301 tttgaatcaa gtcttcaatc tctgcatcca aaacttcctc aacaaagaca attccaaaaa
    36361 tttctaacat atctgcaaga gcttgcttga cacttgcatc atagttccct gagttgatcc
    36421 atttggtcat ttcaaagaca actgtgatac cgttggcagc attaaaatct tcatccatag
    36481 ctgctacaaa cttatcttta aagttttgta actcttgggc atccacgttt cctgtaaatg
    36541 gttgttcgta agtattcttc agatacttga gattggtctc ggcatcgcga actgcctttt
    36601 ccgtgaagtt gataggctta cggtagtgct gggtcgcaaa gaagaaacga agtacttgcc
    36661 catcaagagt tttaagggca tcgtgtaccg taatgaagtt acccaaggac ttagacattt
    36721 tgacattgtc gatattgaca aagccattgt gcatccagta gttagcaaaa gccttgcctg
    36781 ttttagcttc agattgggca atttcattgg tgtggtgcgg aaattctagg tcagctccac
    36841 caccgtggat atcaatggta tcacctaaaa tctctgtcga catgactgaa cactcaatgt
    36901 gccaacccgg acgtccaggt ccccaaggac tatcccaaga aatctcacct ggtttggaag
    36961 atttccatag agcaaaatct acaggatttt ccttacgagc cgtttcttca tcggtacgac
    37021 ctgaagcacc tagatccaaa tcttccaagg ttttattagc caatttggca tagttgtggg
    37081 atttttctac acggaaatag acatcccctt gactctcata ggcaaagcct ttctcgatca
    37141 agtcttccac aaaacggatg atgtctgcca taaactccac tacacgcgga tggcgagtcg
    37201 caggtttcac gcctaaggcc gtcacatcct cacgaaaggc agcgatgtac ttatccgcaa
    37261 cctcctgagg cgtgatacct tcttccctgg cacggttgat aatcttatca tccacatctg
    37321 taaaattgga aatataggca accttatacc cacggtactc aaaatagcga cgaatcgtat
    37381 caaaagctac cgtcgaacgg gcgtttccca cgtggatata gttgtacacc gttggcccac
    37441 aaacatacat cttgatcttg ccgtcctcaa tcgggacaaa ttctcgcaaa tcacgagaca
    37501 tggtgtcata gattttaatc ataaatcata atcaggaaag ctgaaatcca agaacaatta
    37561 gtttcatcac taaaagttca agtaaatttc agtccgaata tctctacact tcggaatccc
    37621 ttgctccttt ctcattcaga taaaccacct gagtctgttt gacaaagcca attttttcat
    37681 acaaacgttt ggcacctaca ttgctatctt ccactgcaat ctgaaattcc ttgtcatttt
    37741 gctcaattag ttggttgacg agggattttg ctaagtagct tccatagcct ttcccacgtt
    37801 caggttccga tattgctaaa ccgtagaagt aattcgtatt agtcgataaa tctaccgtgc
    37861 aagtcccaat aacctgaccg tcttttaaca aaatatataa gcgactttct ggatccttca
    37921 gagcttcagc gacatatcta tccacaactt ctctcgattc atgttcctct gaaaatgcct
    37981 gaaattttaa ttgactaatt tgatcctgat acgaactatc tgctaacaaa acttcaagat
    38041 gggaaacatt tgctaacgga taaggtcttc tatccttacc taaccaagtt tctgtctctt
    38101 catcctcgat tagtccccag ttactggcaa agtcaggatg attctctaaa aaaatacgtt
    38161 ctgtctgaaa agtgactgac cgaatgggga aagaagctat ttctctctca aaactagtaa
    38221 acaatgcacg cgcaatcccc tgacggcgat gacctggatg aaccagtatc gttacttcta
    38281 catcttggtc atctgcatag acagttaata aaccaacaag ttcgcctttt tcataataaa
    38341 ggaaaaaggc gggcatgttt gggtcaaaat taagcatgtt agagagatag ggatcgcgat
    38401 aggtaccgtc atagttttgg caacagttaa ttactttttt cgcctcagat agctcctctt
    38461 ggcttaactt gtttcttgct tgaatcatat aggtatcctc tacaaaccag acgatctgtg
    38521 actggcatct ttagcctgct cgagtttatt gacataatac tctcgttttt cttcgacttc
    38581 gtgaatgaca ggctcatctt tcttaccatg aagacggaca atcttggccg gaataccgac
    38641 aaccgtcacg tcactaggta catctgctac gacaactgct gcagcaccga ccttggcatt
    38701 ttcaccaatt tccacaggcc cgataacttg ggcatgggct gatatgaggg ctccctttcg
    38761 tacagtcgga tggcgtttgc cacagtcttt ccctgttccc ccgagagtca ctccgtgata
    38821 gagaagaacg cctttttcaa caatcgctgt ctctccaatc accagaccag aaccatggtc
    38881 aataaaaaca cctgaatcaa tctgggctcc tggatgaatc tcaatctgag tccaaaagcg
    38941 ccaaaactga ctgtacatac gagctaatag tttgaagccg tgcttccaga gaaaatgcga
    39001 gagacggtgg gccgccaagg ccttgacacc tggataagtc agcaaaacct ccaaagtggt
    39061 gcgggccgct ggatcatttt cttttacaat atcaatggtt tcgcgccacc accccataca
    39121 tttctccttt tcttattctg aatcttttgg tgtttctgta aattctttct taggtttgtg
    39181 atccttttga tgacgtgggc ggtgagggcg ctcagacttt tcaccttttt catcatgctc
    39241 aggttttggc ggacgaggta gaagagcctt catagaggca tcgatacggc ctttttcatc
    39301 aatcttgata accttaacat caacttcatc cccgatttct accaaatcct ctacacgatt
    39361 ggtacgagtc caagccatct cagagatatg aacaagggca tctgtcttat caaagaggtt
    39421 aacaaaggca ccaaatttct cgatacgaac gactttagca cggtaaactt catccacttt
    39481 ggcttcacga accaaaccag caataatttc tttggcacgg ttaatagcat cttggtcact
    39541 agagtagata gacacatttc cttcttcgtc tatatcaatc ttaacacctg tttcagcgat
    39601 aatcttgtcg atggtttctc cacccttacc gatgacaatc ttaatcttgt ccacatcaat
    39661 cttgatcgta tcaattttcg gagcagttgg agccaattct ggacgaactt ctggaatggt
    39721 tgcttcaatg acatcaagga tttcaaaacg cgctttcttg gcttgagcaa gagcctccgt
    39781 caagatttct gcagtaatcc cttgaatctt gatatccatt tgaagggctg taatcccatc
    39841 acgagtacct gcaaccttga agtccatatc tccaaagtga tcttccaaac cttggatatc
    39901 tgtcaatact gtgtagttat ttccatctga gataagtccc atagcaatac cagctactgg
    39961 cgccttgatt ggcacaccac cagccataag ggcaagagtt cccgcacaga tagaagcttg
    40021 agatgaagaa ccgtttgatt ccaaaacttc tgctactaga cggatagcgt atgggaattc
    40081 ttccaagctt ggcaagactt gagcaagagc acgctcacca agagcaccgt gaccgatttc
    40141 acgacgacct ggcgcaccgt aacgacctgt ttcccctaca gaatattgag ggaagttata
    40201 gtggtgcata aagcgtttct tgtactctgg atccaaacca tcaatgattt gagtttctcc
    40261 catcggagcc aaggtcaaga ctgaaagagc ttgagtttgc ccacgagtaa agagacctga
    40321 accatgtaca cgaggaagga agtcaacaac cgcatccaaa ggacggattt catcgacctt
    40381 acgaccatca ggacgcacct tgtcttctgt aattaaacgt cgcacttctg cgtgttccat
    40441 ttgttccaag atttcagcca catcacgcat aatacggtca aattcttcgt ggttcgcata
    40501 tttttcttcg taaacggcag tcacttggtc tttcactgct tgagttgcag cttcacgggc
    40561 caatttctct tctacttgaa ctgccttttg gaggtcactg ttgtaggctg caatgatttc
    40621 agcttgcaat tcagcatcca cgtgaagcaa ttccacttct gctttttctt taccgacagc
    40681 agcaacgatt tcttcttgga aggtaatcaa ttctttgaca gcttcgtgcc ctttaagaag
    40741 cgcttccaac atgatttctt ctgacaattc tttggcacca gactctacca tgttgatagc
    40801 gtgcttggtt ccagctactg tcaattcaag aagagattgc tctgcttgtt cttgacttgg
    40861 gttgatgatg atttggccat ctacatatcc cacttgtacc ccagcaattg gtccgtcaaa
    40921 tggaatatct gaaatagaca gtgccaaaga tgaaccaaac atagcagcca ttggtgcaga
    40981 tgcattttca tcataagaaa gcactgtatt gatgacttgg acttcattac ggaaaccttc
    41041 cgcaaacata ggacgaatcg gacggtcaat caaacgcgct gtcaaggtcg catctgttga
    41101 aggacgtcct tcacgtttca taaagccacc aggaaacttc ccagccgcat acattttttc
    41161 ttcgtagttg acttggagtg ggaagaaatc cccagttgcc attttcttag acataacggc
    41221 agcagtcaag acagttgact caccgtaacg tacgacaaca gagccatttg cttgcttagc
    41281 aacctgacca gtctctacaa ttaactcacg acccacaaaa gtcgtttgaa acacttgttt
    41341 tgccatttta atcccctttg gattgatgaa attatacgcc ttgcctacaa agatcaagat
    41401 accaaggacg tcaaaagcaa agtaaaaata ggaaactgac gaagtcttcg atgaagacaa
    41461 gacagtttat cttttttaca cagcttttcg gccgtgttca attacacaag atattttgga
    41521 cggttcggct tgccgaacat ttctgtagaa aaataggaag gtgacgtcgc actcgacgag
    41581 tgctaggaag cttatctttt ttcctaagaa atgagaccaa aattcaatta ctcaagatgc
    41641 taagccgtca agcaactcaa aggaagatag gaaatcgaac gacggagcga ctgctcctag
    41701 ggagatttat ctttttccac agagttgtag gcaagttcag ttttcaagat acatcattag
    41761 aaaggtttaa tattaaagta tctaaacctt tcacgctaat tgctatcggg cgattagcta
    41821 aatgctttac taactctttc gtcaaataac atcgatttga ctcactcgtg tcgttaaatc
    41881 ttacagttta aatgcattgt attatttaat accttcatct ttgtatcaag tacgtacaga
    41941 atctatttta tcatattttt cttaaaaagt gaggtctttg ccattaaaaa ggaaccattc
    42001 ccctcacctg agaagaatgg tttgctttta ttatcctaga gactggtgat taaacaaggc
    42061 atgggttgct tgatggatgt attttgctgt atcagcatta ttcatcgtat agagatgcac
    42121 accggcaaca tcctgagtta ccaagtccac gatttggtcc actgcatagg caagtcctgc
    42181 tgctctgagc gactcagggt catgctcata cttgtctaag atggctttaa atttgcgtgg
    42241 aagatggata ttctcacaag tcttcaagag tcggagagcc tgatttcgat tcagaattgg
    42301 cataattcct gcatgaatgg gaacatcaat cccagccaag atgcacttgt cctgaaaatc
    42361 atagaagcgc tcattgtcaa agaagagctg agttacgagg ctcgaacagc ctgcatccac
    42421 tttcttctta agattttgaa tatctgaaat ctgatttggc gaatctggat gcccttctgg
    42481 atagcaagct ccaataatat caaagtgagg ggtttgttcc ttgataaact caatcaagtc
    42541 ggttgcatag cggaaatcct tttgtggttc cacgtctgga ataatatccc cacgaagagc
    42601 caagattttc tgcaccccaa ctttgtccaa gtcagcaata gtttcagcaa ccttgtcctt
    42661 agttagataa atagctggca agtgggcaat ggtcggaatc gccaaatcat tttggataaa
    42721 gtcagccaaa cgaaccgtcg tttccttgat attaaattta ttattgctgg cagttacact
    42781 gataaaatgg ggagccaact cctgcatatc ctgcaaggct gaaataatgt taccattacc
    42841 cacggctggg tttggaggga acacttcaaa tgagagtgac ggtgtttggc gtgacatatg
    42901 taataacctt ttctagttga tttctttttg aacaaccact gtatggagag aaatccaatc
    42961 ttacaatttc tcacgcgcag ctttagctgc ttcaacaagg cggatcaagc tttcttttgt
    43021 ttctgggata ccacgtgttt tcaaaccaca gtcagggttg atccaaactt tcttgcttgg
    43081 cactttagca aggatgactt cgattgtgtt gtcgatttcg ccttcatttg gtacacgagg
    43141 tgagtggata tcgtaaaccc caggtcccac ttctgtttgg aagtttttcg ctttgagtgc
    43201 gtccaagatt tcaaggtttg aacggctagc ttcaaaggaa ataacgtctg catccatgtt
    43261 atcgatagct gggatgatat ctgtaaattc tgagtaacac atgtgagtgt ggatttgtgt
    43321 gtctggcgct actgttgagt gtaccaagcg gaaggcagga atagcccagt caaggtagtc
    43381 ttcgtaccag tcgctacggc ggagtggcaa tttttcacga agagcagcct cgtcgatttg
    43441 gatgattttc acaccagcag cttcaaggtc aagtacttca tccttgatag caagggcgat
    43501 ttggagagtt gaatccttga tagagatgtc ttcacgtggg aatgaccagt taaggatggt
    43561 aacaggtcca gtcaacatac ctttaacagg tttgtttgta cgactttgtg catagctaga
    43621 ccatttaaca gtgatagggt taagacgagt gacatcaccc cagatgattg gtggttttac
    43681 cccacgcata ccgtatgatt gtacccatcc atttttagag aagaggtatc ctgacaagtt
    43741 ttgaccgaag tactcaacca tgtcattacg ctcaaattca ccgtgaacaa ggacatcaaa
    43801 gtcaatatct tcttgccact tgatccattc gtcaatcgtt tcagcaagga aagcgtcgta
    43861 ctctttttga gacaattcac ctttacggta agccaaacgt ttggcacgaa cttcttttgt
    43921 ttgagggaat gaaccaatcg ttgttgttgg aagagctgga agtttgaaag cttcttcttg
    43981 gatagcttca cgttctgcaa aggctggcaa acgagtgtag tctgcgtctg tcaagccagc
    44041 gatacgcgca cgaagttcag cattttcacc aacacgctca gtcgcaaaga gttctttgtt
    44101 ggctgcaaga gcttctgaac cttgaccatt tcggatagca tccaagtcac ggatttcatc
    44161 caatttttca actgcaaagg caaagtggtt caagagtgct ggttcaaatt cttcattagc
    44221 agttgtaaat ggcacatgaa gaagtgagca agagcttgtc aaaacgatgt tttcagctgg
    44281 gatttgctca agaacagtca agctcttttc gtagttgttg cgccagatgt ttttaccatt
    44341 gacaatacct acatagagag tcttgtcagc tgggaagcca cctttaacga gttcaagagt
    44401 tttcttacct tcaacaaagt caagaccgat agcatctact ggtaagttta caaggtcagc
    44461 gtatacgtca cgaacatcac cgaaataagt ttgaagcaag acttcaagac cttttttgtc
    44521 agccaagagt ttgttgtaaa ggttcaagaa aagagctttt tcttcagctg tcaagtcttt
    44581 tacaagagcc gcttcatcca attggatgcg agtcgtacca agttcagcca atttagcaaa
    44641 aacttcttgg taagcagaca ctaagctatc tacgaagtcg tctgctttca cgccttcttc
    44701 aaagtctgac aattgaagga aagtgaaggg acctacaaga acaggacgag tgttcaatcc
    44761 aagttctttg gcttcttgga actcatcgaa aatcttgtga ccagccaatt ttacttgagt
    44821 gtctttttca aatttaggaa cgatgtagtg gtagttagtg ttgaaccatt tcttcattgg
    44881 aagggcgcga acgtcccctt tttctccctg gtaaccacgt cccaaagcga agtagcgctc
    44941 aaggtcagac aagtccaagt tttgaacgga tgcaggcacc acgttgaaaa ggaaagccgc
    45001 atctaggaag ttatcatagt gagaaaagtc atttgatgga atttcagtga tgcctttttc
    45061 tttgacaatg ttccagtgtt tagcacgcaa gtcttttgct gctgctaaaa gttcttcttc
    45121 tgagatttct tttctaaagt atttttcagt tgtaaatttt aattcacgga attcgcccaa
    45181 acgagggaaa ccgatgattg tagttgacat gatgtgtcct ccaaaatttg ttgttgaaac
    45241 tatcttaaca gaaaagaaag cgtctgtata attgtaaaaa attagggttt gatatagttt
    45301 gaaactatat atctgtttcg gacaaaagaa aaagacttga agcaaacgtc tcaaatcctt
    45361 tgtaattctt actttacagc tatattccaa ttagaatact aaaacatgtt attagtaatt
    45421 cttataagtg actatgacct gttattagaa aagactataa ctgattctag tcaacttttt
    45481 ccctgttcaa gtgggacgac tgctagtgtc tttcctaaac tggctaggac ttttaagact
    45541 gtatccaact gaggactagt ctttcctgtc tccatcctag ctatgacagg ctggcttatt
    45601 ccactgactt cttccagctt tttctgactg attccttgtt catacctagc ctcaatcaac
    45661 tcgctcatga tagccactcg catatcactt tcaagaattt cctccttgct aaagagctca
    45721 gatggacatc cttccaatta ctcccaatag cactattctt catcacttaa ccctcttttt
    45781 tttacgtcta tgtattttta aaaaaatgag cgaattatga ttcgatagat tgaccagtgg
    45841 gtttaaagtt ggtgctagcc tatttcttaa gcgattttcc ttttctagga taaagcatat
    45901 cctgcttgct taaccccaat tttccacgat gaatccaata gtaaatggtt gaaattccca
    45961 cgttaacccc tttaaccata accatcattt caggcgaaaa tttttggtta tgtatagtgg
    46021 agaatctttt cctttagttt cttaagactg ttgagcgtag tcggcagaat aaacctcttt
    46081 gaagcgccct tttccaagac attgtcggac tgtcccacgc tttatttcag tgtggatagt
    46141 ttgaggagct tttccaagta gagaggcaat ttctctattt gattttcctt cttttttcca
    46201 tcgttcgatt aagcgacggc tatcgattgt caaatgtttg ccttttgtag tataattgtc
    46261 ttgcatttct gtgcctttta atcatttcaa tcttaaattg gacttttttt acttgggttg
    46321 tacttaatct ataaggaaga caataaaaag aatatcaatc aagtaaagtc acaaagtcac
    46381 attagctccg atcaaccatt gcaaattgag gtactcacac aatgattaaa acatttctct
    46441 ctgccctttc ggtcattctc tttgttatcc ctatcatagc ttattctttt tttccatctt
    46501 ctaatcttaa taattggcta tctaccagac ctatcttggc acagatttat gccttcccct
    46561 tagctactgc aactatggct tctattttaa gtttcgtatt tttttcccta tctttttaca
    46621 agaaaaataa acaaatacgg ttttactctg gcattttgct cttactatcg ctcatattac
    46681 tattattcgg aacagataaa accctttctt ctgcatcaaa taagactaaa accttaaaat
    46741 tagtaacttg gaacgtcgct aatcaaatag aagcacaaca tattgagcga atttttagcc
    46801 attttgacgc cgatatggct atattccctg aactagctac caatatcaga ggtgagcaag
    46861 aaaaccagag aatcaaacta ttgtttcatc aagttggact ttctatggcc aactatgata
    46921 ttttcacttc tccacctacc aatagtggaa tagctcctgt gactgtgatt gtcaagaaaa
    46981 gttatggttt ctatacagaa gctaaaactt ttcatacaac acggttcggg acaattgtat
    47041 tacattcgag aaaacaaaat ataccagata tcattgcctt gcatactgct cctcctctgc
    47101 caggtttaat ggaaatctgg aagcaagact taaacatcat tcataatcaa ttggcttcaa
    47161 aatatccaaa ggctattatt gcaggtgatt ttaatgcaac tatgcgtcat ggagcacttg
    47221 caaaaataag ctctcatagg gacgcattaa atgtactgcc accttttgaa agaggaactt
    47281 ggaatagcca aagtccaaaa ctttttaata caacaataga tcatatttta ttgcctaaaa
    47341 accactacta tgttaaagat ttagacattg taagttttca aaactctgat catagatgta
    47401 tttttacaga aatcacattt taattatttt atataaaatc acccctctag tgttcataaa
    47461 ctagaggggg aatttgtatc ctactatcgt ttaacgcact tctgcattga ctttttcttc
    47521 gagagacgct tggatttttt ccatgtagcg tgcgacttct tcgtccgtta agctgtcttc
    47581 tggattttgg aaggtcaagc tataagccat tgacttcata ccaagtccca gtttttcacc
    47641 tgagaagacg tcaaagagtt tgatatctgt caaacgtttc acgccggcag cttggatagc
    47701 atctacaact tcttggtgag tcacttctgc cttgaggaga agggcaacgt cacggctgac
    47761 tgctgggaat ttcgtgattt ccacaaatgg agccgctggc tgaagcgcag cttcgatagc
    47821 tgaaaggtta agctcagcta catacgtttc tggaatatca taagccttag cagtgactgg
    47881 atgcacttgg ccaaggaaac caagaacttg gtcaccgagt gaaatcacgg ctgtacgacc
    47941 tggatgaagg ctagcgattt cagatgttgc tgtataggtt acttggagtc ccaaacgagc
    48001 aaatagcgct tcaaggattc ccttagcata gaagaaatca actggaactg ctactgtttg
    48061 gaaatctttt tcagcaacca agcctgtcaa ggcaaaggca aaactgttga tttcatttgg
    48121 aagttcttct cttggattac ctgtttgttc aaagactttt ccaatctcgt aaagggccaa
    48181 gtttttattc ttacgagcca cgttgtaggc aacggtatca aggatacctg aaatcatatt
    48241 ttgacggagg actgaacgat ccacagtcat tggccacata agttcagtaa ggttacttgg
    48301 ttgagccgta aactcaactg ctttttcagg agttgttaga gtataggtga tgatttctgt
    48361 caaacctact ccttcagcaa tagtacgaac ttgacggcgg agtttttgtg tggctgtcaa
    48421 ttcacctgct gtaccatcgt attttggtag actagttggc aagcggtcat aaccatagat
    48481 acgagcaatt tcttcaaaga ggtcagcttc gattgtgata tcccaacgac gacgtgggac
    48541 tctgactgta aagctgtctg catttccaga aagaccaaag ccaagacgac ggaagacgtc
    48601 ttctacatca gcataagaca gctcagttcc gaggacacgg ttaacatcag caagggttga
    48661 agaaacttct acatctgaag tatcaagctc acccgctgaa acgatgccct tacgcaccgt
    48721 cgcacctgca agttccgcaa tcaggctagc tgccgcatca agggcttcat taactgttgc
    48781 cacattaatt cctttttcaa agcgagaaga tgactcagaa cgaaggttca ggcgaccact
    48841 tgtcttacgg atagatttgc cattgaaaac agcagcttca aggacaacac gactagattt
    48901 ttcagagatt tctgttgctt gaccacccat gacacctgca agggctactg gcttgtctgc
    48961 gacagtgatg actaggtcat tcacgtccaa gtcacgttct tcaccgtcca aggtcaccaa
    49021 tttttcacca gcacgcgctt cacgcacacg gatgtcagtc ccttcaaagt tatccaagtc
    49081 aaaggcatgc attggttgac caaaatagag caggatgtag ttggtcacgt ccactacgtt
    49141 attgatggga cggattcctt cgttcatgag aaggttttgc aaccattgtg gacttggtgc
    49201 gatagtcaca ttgtccaaga tacgagctgc atagtaaggc gccttgtctg tcccaatgat
    49261 gacagaaagg gcatctgccg cagcttcatt agtttctgtt agagtaaatt ctttaaagtt
    49321 gactgccttg tcatagatgg ctgccacttc gtgagccact ccacacatag aaagggcatc
    49381 tgcacggttt ggtgtgatgg aaagttcgat gatttcatca tccaagtcta ggtaagaaaa
    49441 gacttcctca cctggcacgg catcttcagg caagatttgg atgccatctg cgaattcctt
    49501 aggcacaact gagtcagaaa tccccaattc accaagtgaa cagatcattc caagtgactc
    49561 caaaccacgg atttttcctt ttttgatttt gtagttatca gcgatacgag ctcctggaag
    49621 agccaccatg accttgatcc cagcacgcac atttggggca ccacaaacga tctgacgctc
    49681 ttcttcttcg ccaacgttaa cctgacaaac atgaaggtga gtctctggca catcttcgca
    49741 agacaagacc tcaccgacga caatttttga gagaccagca gctggtgatt cgacaccctc
    49801 tacctcgatt cctgtagttg acattttttc agccaactct tgtgatggca catcaatgtc
    49861 caccaattct tttaaccatt tataagatac aagcataatt tagttctcca gaatgacagt
    49921 tgtcactcta gttcttttcc tttcctatca tttcaataga agaatcctct tcttacctta
    49981 atttctttct cagtaaccaa tccgtatcta ctttttgacc aaccataaaa tgatgttggc
    50041 taaatttttc aaaaccatat cgattataaa acgcttgagc ttttgtatta tgctcccaaa
    50101 cacctaacca agcccaagaa aaactatttt ttgtagcaag ttcaagtgcg aattcaaaca
    50161 gttgcttacc tagtccaaat ccttggaatt tttgtagcac atagaggcgt tgaatttcaa
    50221 aagcgtcctc taattctctc tcagtttgag cacttcccca gttgactttg agaaaaccag
    50281 ctatctcctc ctcatgcata atgaaatagg tttcagagtc aggatttccc aactcagttg
    50341 acaaagtttt tagactataa gcctcttcaa agtattcctg taactgctct tccgtattat
    50401 catacgcaaa ggtttcacga aaggtttgtt tggcaatttt agccaacacc tcaacatctg
    50461 ccacttctac ttttctaatc attatttaaa ctgttctgag aagcggacat ctccttggta
    50521 gaatccacgg atatcgttga ttccataacg gagcatagct acacgctctt gtccaagacc
    50581 aaaggcaaag ccagagtata cagtcgcatc gataccactc atttcaagga cacgtgggtg
    50641 aaccataccg gcccccataa tttcgatcca acctgttttc ttacatacgt tacagccttc
    50701 tccaccacat ttgaagcaag aaacatccac ctcaacagat ggctctgtga atggaaagta
    50761 agatggacgc aaacgaattt gacgctcttc accaaacatt ttttggacaa tcaactgaag
    50821 cgttccttga agatcagcca tagagatatt tttcccaact accaagcctt cgatttggtg
    50881 gaattggtga ctgtgggtcg catcgtccgt atcgcgacgg aagacacgcc ctggcgagat
    50941 catcttcaaa ggacctttag aaaaatcatg ggcatccata gcacgcgcct gaactggaga
    51001 cgtgtgggta cggagcaaga tttcttcagt gatatagaaa gtatcctgca tatcacgagc
    51061 tgggtggtct tttggaaggt tcatacgttc aaagttatag tagtcttgct ccacttcaaa
    51121 accatccacg acttgataac ccataccgat gaagatatct tcgatttctt cactggtttg
    51181 tgtcaaaacg tgacggtgac cagtcgcaac tggacgacct ggaagcgtca catcgatact
    51241 ctcgctagcc agttgagccg cgactttctt ttcttccaag agcttagctg tttcttcaaa
    51301 agcagctgtc aagacatcac gagcttcatt gacgtgtttc ccgatgattg gacgcatctc
    51361 agcagaaaca tctttcatcc ctttgaggat ttcagtgagc gaaccctttt taccaaggac
    51421 agagacacgc aaatcttgca tctctttttc atttccagca gtaatctgct tcaagctagt
    51481 cagcgtttct tcgcgaagcg cttttaattg ttcttcaata gttgacatat ttcctccatc
    51541 agtctctcgt agataaaaag aaaaccacat gccaaaaact ccactcggag cgttgacacg
    51601 cggtaccatc cgttttcatc tgacaagtca gaccttcatt tctaaatcca tgcgcaagtg
    51661 aattcaccca gctttcatat agagagcttg cagtcacggc tctcctccct gatatacttc
    51721 ccttgggcta ctagtctttc agattcctat tcaattacta cttagtttat cagattttta
    51781 ccattcttgc aagacctatc ttacttctgc ttgttagctt attcttatct aactttatag
    51841 cggattgaaa t
//
LOCUS       DAVKJJ010000007        51082 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000007 DAVKJJ010000000
VERSION     DAVKJJ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 51082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51082
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.007"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            33..227
                     /locus_tag="VW144_000392"
     CDS             33..227
                     /locus_tag="VW144_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9599888.1"
                     /translation="MEAEAIRRNCERGAIFSGKIKYHEDSQFKGDHYVECYAVLDNTV
                     IARDRITVPIDPLCGKDFIE"
     gene            472..1806
                     /gene="trmFO"
                     /locus_tag="VW144_000393"
     CDS             472..1806
                     /gene="trmFO"
                     /locus_tag="VW144_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEU9599889.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            1890..2627
                     /locus_tag="VW144_000394"
     CDS             1890..2627
                     /locus_tag="VW144_000394"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU9599890.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            2636..3193
                     /gene="frr"
                     /locus_tag="VW144_000395"
     CDS             2636..3193
                     /gene="frr"
                     /locus_tag="VW144_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU9599891.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            3253..4107
                     /gene="cvfB"
                     /locus_tag="VW144_000396"
     CDS             3253..4107
                     /gene="cvfB"
                     /locus_tag="VW144_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEU9599892.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            4116..4331
                     /locus_tag="VW144_000397"
     CDS             4116..4331
                     /locus_tag="VW144_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEU9599893.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            4417..5385
                     /locus_tag="VW144_000398"
     CDS             4417..5385
                     /locus_tag="VW144_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU9599894.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQAIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(5363..5542)
                     /locus_tag="VW144_000399"
     CDS             complement(5363..5542)
                     /locus_tag="VW144_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9599895.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLRPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            5578..6078
                     /locus_tag="VW144_000400"
     CDS             5578..6078
                     /locus_tag="VW144_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9599896.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            6081..6341
                     /locus_tag="VW144_000401"
                     /pseudo
     CDS             6081..6341
                     /locus_tag="VW144_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(6708..7034)
                     /locus_tag="VW144_000402"
     CDS             complement(6708..7034)
                     /locus_tag="VW144_000402"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9599897.1"
                     /translation="MVDVAVDQGGVIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(6980..7393)
                     /locus_tag="VW144_000403"
     CDS             complement(6980..7393)
                     /locus_tag="VW144_000403"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEU9599898.1"
                     /translation="MGAHFLTKQAGGSGVLLGGVPGVPKGKVTIIGGGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLMSNSFNIEASVRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            complement(7394..7819)
                     /locus_tag="VW144_000404"
     CDS             complement(7394..7819)
                     /locus_tag="VW144_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEU9599899.1"
                     /translation="MLIGIPKEIKNNENCVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDDLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            7996..8565
                     /locus_tag="VW144_000405"
     CDS             7996..8565
                     /locus_tag="VW144_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9599900.1"
                     /translation="MESIFVKLAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKDNPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            8633..9283
                     /locus_tag="VW144_000406"
     CDS             8633..9283
                     /locus_tag="VW144_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU9599901.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSIDELKKVSGIGAKTIEKLKDYVTVD"
     gene            9267..11507
                     /locus_tag="VW144_000407"
     CDS             9267..11507
                     /locus_tag="VW144_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEU9599902.1"
                     /translation="MLQWIKNIPIPLIYLSFLLLWFYYAIFSASYLALLGFVFLLVCL
                     FFQFPWKSASKVLVICGIFGFWFVFQNWQQSQASQNLADSVEGVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVARRPLVFGQPNAWLLILLLI
                     SLALVYDLRKNIKGLTVLSLLITGLFFLTKYPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     LEVTKAFHVGEILVSKGSLKQKQFVVELQATQTKVRSMTVGENLPIFGSQLEVLSLRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPTFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            11906..12493
                     /locus_tag="VW144_000408"
     CDS             11906..12493
                     /locus_tag="VW144_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9599903.1"
                     /translation="MLQLTHVTLKTRQVILQDADFTFKKGRFYGLLAINGSGKTTLFR
                     AISNLIPISSGNIAAPPSLFYYESIEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            12497..13678
                     /locus_tag="VW144_000409"
     CDS             12497..13678
                     /locus_tag="VW144_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9599904.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILALFVSVLGVTFYFNSQTANSVS
                     LERELETRLVDRERVINEYEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKDYEFVSNDPTASSELKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEAIIPTLFVIGIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYSIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFIESWGSSRKKEVFK
                     V"
     misc_feature    13838..13969
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            13985..14515
                     /gene="infC"
                     /locus_tag="VW144_000410"
     CDS             13985..14515
                     /gene="infC"
                     /locus_tag="VW144_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU9599905.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            14548..14748
                     /gene="rpmI"
                     /locus_tag="VW144_000411"
     CDS             14548..14748
                     /gene="rpmI"
                     /locus_tag="VW144_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU9599906.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            14800..15159
                     /gene="rplT"
                     /locus_tag="VW144_000412"
     CDS             14800..15159
                     /gene="rplT"
                     /locus_tag="VW144_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU9599907.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            15217..15597
                     /locus_tag="VW144_000413"
     CDS             15217..15597
                     /locus_tag="VW144_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9599908.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            15847..16647
                     /locus_tag="VW144_000414"
     CDS             15847..16647
                     /locus_tag="VW144_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEU9599909.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            16658..17596
                     /locus_tag="VW144_000415"
     CDS             16658..17596
                     /locus_tag="VW144_000415"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEU9599910.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEYAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            17638..19539
                     /locus_tag="VW144_000416"
     CDS             17638..19539
                     /locus_tag="VW144_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEU9599911.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKMAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYM
                     AKNETVDGYQLGSDGKWLGGKTTNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWL
                     DKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIP
                     VASHLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINN
                     SLLENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYL
                     SAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMK
                     INEKLGGKD"
     gene            complement(19600..21255)
                     /gene="pavA"
                     /locus_tag="VW144_000417"
     CDS             complement(19600..21255)
                     /gene="pavA"
                     /locus_tag="VW144_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEU9599912.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTKSLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            21360..21857
                     /gene="ybeY"
                     /locus_tag="VW144_000418"
     CDS             21360..21857
                     /gene="ybeY"
                     /locus_tag="VW144_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU9599913.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAKKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            21838..22233
                     /locus_tag="VW144_000419"
     CDS             21838..22233
                     /locus_tag="VW144_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEU9599914.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFGFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            22250..23149
                     /gene="era"
                     /locus_tag="VW144_000420"
     CDS             22250..23149
                     /gene="era"
                     /locus_tag="VW144_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU9599915.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            23198..24022
                     /gene="mutM"
                     /locus_tag="VW144_000421"
     CDS             23198..24022
                     /gene="mutM"
                     /locus_tag="VW144_000421"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEU9599916.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDTYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            24022..24627
                     /locus_tag="VW144_000422"
     CDS             24022..24627
                     /locus_tag="VW144_000422"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU9599917.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQAVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSNQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            24614..25813
                     /locus_tag="VW144_000423"
     CDS             24614..25813
                     /locus_tag="VW144_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEU9599918.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRIFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            25813..25962
                     /gene="rpmG"
                     /locus_tag="VW144_000424"
     CDS             25813..25962
                     /gene="rpmG"
                     /locus_tag="VW144_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9599919.1"
                     /translation="MRVKINLKCSSCDSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            26002..26235
                     /gene="secG"
                     /locus_tag="VW144_000425"
     CDS             26002..26235
                     /gene="secG"
                     /locus_tag="VW144_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU9599920.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            26337..28691
                     /gene="rnr"
                     /locus_tag="VW144_000426"
     CDS             26337..28691
                     /gene="rnr"
                     /locus_tag="VW144_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEU9599921.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQTLGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVSSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDTVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKL
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            28654..29121
                     /gene="smpB"
                     /locus_tag="VW144_000427"
     CDS             28654..29121
                     /gene="smpB"
                     /locus_tag="VW144_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU9599922.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            29137..29997
                     /gene="tehB"
                     /locus_tag="VW144_000428"
     CDS             29137..29997
                     /gene="tehB"
                     /locus_tag="VW144_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEU9599923.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            30070..31023
                     /gene="coiA"
                     /locus_tag="VW144_000429"
     CDS             30070..31023
                     /gene="coiA"
                     /locus_tag="VW144_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEU9599924.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            31042..32844
                     /gene="pepF"
                     /locus_tag="VW144_000430"
     CDS             31042..32844
                     /gene="pepF"
                     /locus_tag="VW144_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9599925.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALVVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDCDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            32846..33559
                     /locus_tag="VW144_000431"
     CDS             32846..33559
                     /locus_tag="VW144_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEU9599926.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQRLFDATLDNSELTATLVPLG
                     DGILMLRKNVADVQLPDSE"
     gene            33626..34567
                     /gene="prsA"
                     /locus_tag="VW144_000432"
     CDS             33626..34567
                     /gene="prsA"
                     /locus_tag="VW144_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEU9599927.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(34686..35027)
                     /locus_tag="VW144_000433"
                     /pseudo
     CDS             complement(34686..35027)
                     /locus_tag="VW144_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            35441..35536
                     /locus_tag="VW144_000434"
                     /pseudo
     CDS             35441..35536
                     /locus_tag="VW144_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(35789..36409)
                     /locus_tag="VW144_000435"
     CDS             complement(35789..36409)
                     /locus_tag="VW144_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9599928.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGSESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTPNTWNDTSYQS"
     gene            complement(36409..36891)
                     /locus_tag="VW144_000436"
     CDS             complement(36409..36891)
                     /locus_tag="VW144_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEU9599929.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(36891..37733)
                     /locus_tag="VW144_000437"
     CDS             complement(36891..37733)
                     /locus_tag="VW144_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEU9599930.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(37828..38628)
                     /locus_tag="VW144_000438"
     CDS             complement(37828..38628)
                     /locus_tag="VW144_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEU9599931.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            38772..40151
                     /gene="glmU"
                     /locus_tag="VW144_000439"
     CDS             38772..40151
                     /gene="glmU"
                     /locus_tag="VW144_000439"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU9599932.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            40161..40706
                     /locus_tag="VW144_000440"
     CDS             40161..40706
                     /locus_tag="VW144_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9599933.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTARRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            40718..41032
                     /locus_tag="VW144_000441"
     CDS             40718..41032
                     /locus_tag="VW144_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEU9599934.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            41049..41741
                     /locus_tag="VW144_000442"
     CDS             41049..41741
                     /locus_tag="VW144_000442"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEU9599935.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNTQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            41818..42309
                     /gene="rocS"
                     /locus_tag="VW144_000443"
     CDS             41818..42309
                     /gene="rocS"
                     /locus_tag="VW144_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEU9599936.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            42407..42997
                     /locus_tag="VW144_000444"
     CDS             42407..42997
                     /locus_tag="VW144_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU9599937.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGVLDLSQLFD"
     gene            43006..44769
                     /locus_tag="VW144_000445"
     CDS             43006..44769
                     /locus_tag="VW144_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU9599938.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
     gene            44983..45625
                     /locus_tag="VW144_000446"
                     /pseudo
     CDS             44983..45625
                     /locus_tag="VW144_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            45792..45992
                     /locus_tag="VW144_000447"
     CDS             45792..45992
                     /locus_tag="VW144_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9599939.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATQAVLLDAQKAPEDSALTE"
     gene            46017..46433
                     /locus_tag="VW144_000448"
     CDS             46017..46433
                     /locus_tag="VW144_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9599940.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            46664..47371
                     /gene="ccdA2"
                     /locus_tag="VW144_000449"
     CDS             46664..47371
                     /gene="ccdA2"
                     /locus_tag="VW144_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9599941.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLTLASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            47382..47939
                     /gene="sdbB"
                     /locus_tag="VW144_000450"
     CDS             47382..47939
                     /gene="sdbB"
                     /locus_tag="VW144_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEU9599942.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIS
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            48313..49704
                     /locus_tag="VW144_000451"
     CDS             48313..49704
                     /locus_tag="VW144_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU9599943.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIALPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKVPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            49847..50764
                     /gene="adcAII"
                     /locus_tag="VW144_000452"
     CDS             49847..50764
                     /gene="adcAII"
                     /locus_tag="VW144_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEU9599944.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            50772..>51082
                     /locus_tag="VW144_000453"
     CDS             50772..>51082
                     /locus_tag="VW144_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9599945.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     EL"
BASE COUNT        15497 a         9304 c        11274 g        15007 t
ORIGIN      
        1 tttcgcagga agtctattgg aaagtaagaa atattgaagc tgaggctatt agaagaaatt
       61 gtgagcgtgg tgctattttt tcaggtaaaa taaaatatca cgaagattca cagtttaaag
      121 gagatcacta tgttgaatgt tatgctgttt tagataatac ggttatagca agagatagaa
      181 taacagtccc tatcgatccg ttatgtggaa aagattttat agagtagcat ataattgatt
      241 cttaactgga atactcacta tctctttaca tcaagaaaat gactaaacag ggaagtttgc
      301 cttcttccct ttttttgtta tactagtaga agaaaaaata gaaagatttg tgggagtgaa
      361 aaagtcctgt ggactttttc agcctgagcc aagaaactcg aaagcttgta agtctgattg
      421 gctttttcaa tgtgaatctt aacttcacac tcccaaagag gtattagtgt cgtgtctcaa
      481 tcttatatca atgttatcgg tgctggtttg gcaggttctg aagcagctta ccaaatcgca
      541 gagcgtggta ttccagttaa actatatgaa atgcgtggtg tcaagtctac accccagcat
      601 aaaacagaca attttgctga gttggtttgt tccaattctt tgcgtgggga tgctttgaca
      661 aatgcagttg gtcttctcaa ggaagaaatg cgtcgcttgg gttctgttat cttggaatct
      721 gctgaggcta cacgtgttcc tgccggtgga gcccttgcgg tggatcgtga tggtttctct
      781 caaatggtga ccgaaaaagt ggtcaaccac cccttgattg aagtggtgcg tgatgaaatt
      841 acagaattgc caacagatgt tattacagtt gtggcgactg gtcccttgac tagcgatgcc
      901 ctggctgaaa agattcatgc ccttaataat ggcgatggct tctatttcta cgatgcagca
      961 gcgcctatta tcgatgtcaa cactatcgat atgagtaagg tctatctcaa atctcgttat
     1021 gataagggag aagcagccta tctcaatgct ccaatgacca agcaagagtt tatggatttc
     1081 catgaagcct tggtcaatgc ggaagaagca ccgctcaatt cttttgaaaa agaaaagtac
     1141 tttgaaggat gtatgccaat cgaagttatg gccaaacgtg gcattaaaac tatgctttat
     1201 ggccctatga agccagtcgg tcttgagtat ccggacgact acacaggacc tcgtgatgga
     1261 gaatttaaaa caccttatgc ggttgtgcaa cttcgtcagg ataatgcagc tggtagccta
     1321 tacaatattg ttggtttcca gacccacctc aaatggggag aacaaaagcg tgtcttccaa
     1381 atgattccgg gtcttgaaaa tgcggagttt gtccgttatg gtgtcatgca tcgcaattct
     1441 tacatggatt caccaaatct tcttgagcag acttaccgtt ccaagaaaca accaaatctc
     1501 ttctttgctg gtcagatgac gggggtggaa ggctatgttg agtcggcggc ttcgggctta
     1561 gttgcgggaa ttaacgcagc tcgtctcttc aaggaagaaa gcgaggttat tttccccgag
     1621 acgacagcga ttggaagctt agctcattac attacccatg ccgacagcaa acatttccaa
     1681 ccaatgaatg tcaattttgg gatcatcaag gagttggaag gcgagcgtat ccgtgataag
     1741 aaggctcgtt atgaaaaaat tgcagagcgt gcccttgccg acttagagga atttttgact
     1801 gtctaatttt tttgaaagaa ttgttcatga tactataaaa atcttagaaa ttgtgataaa
     1861 ataggtagga tgaaagaagg agagtgaaaa tggcgaatcc caagtataaa cgtattttaa
     1921 tcaagttatc aggtgaagcc cttgccggtg aacgtggcgt agggattgat atccaaacag
     1981 ttcaaacaat cgcaaaagag attcaagaag ttcatagctt aggtatcgaa attgcccttg
     2041 ttatcggtgg aggaaatctc tggcgtggag aacctgcagc agaagcaggt atggaccgtg
     2101 ttcaggcaga ttacactgga atgcttggga ctgttatgaa tgctcttgtg atggcagatt
     2161 cattgcaaca agttggggtt gatacgcgtg tacaaacagc tattgctatg caacaagtgg
     2221 cagagcctta tgtccgtgga cgtgcccttc gtcaccttga aaaaggccgt atcgttatct
     2281 ttggtgctgg aattggttca ccatacttct cgacagatac aacagcggcc cttcgtgcag
     2341 ctgaaatcga agcagatgcc atcctcatgg ctaaaaatgg cgtcgatggt gtgtacaatg
     2401 ccgatcctaa gaaggacaag acagccgtta agtttgaaga attgacccac cgtgatgtta
     2461 tcaacaaagg tcttcgtatc atggactcaa cagcctcaac cctctcaatg gacaacgaca
     2521 ttgacttggt tgtcttcaac atgaaccaat caggcaacat caaacgtgtc gtatttggtg
     2581 aaaatatcgg aacaacagtt tcaaataata tcgaagaaaa ggaataagaa agaatatggc
     2641 taacgtaatt attgaaaaag ctaaagagag aatgacccaa tctcaccaat cacttgctcg
     2701 tgaatttggt ggtatccgtg ctggtcgtgc caatgcaagc ttgcttgacc gtgtacatgt
     2761 agaatactat ggagtcgaaa ctcctcttaa ccaaatcgct tcaattacga ttccagaagc
     2821 gcgtgttttg ttggtaacac catttgacaa gtcttcattg aaagacatcg aacgtgcctt
     2881 gaacgcttct gatcttggta tcacaccggc taatgacggt tctgtgattc gcttggttat
     2941 cccagctctt acagaagaaa ctcgtcgtga ccttgctaaa gaagtgaaga aggtcggcga
     3001 aaatgctaaa gtggctgtcc gcaatatccg tcgcgatgct atggacgaag ctaagaaaca
     3061 agaaaaagca aaagaaatca ctgaagacga attgaagact cttgaaaaag atattcaaaa
     3121 agtaacagac gatgctgtta aacacatcga cgacatgact gctaacaaag agaaagaact
     3181 tttggaagtc taaaaataaa cagaaaaact cagttggcat tgctggctga gttttattcg
     3241 aaagaaggaa atatgaatac aaatcttgca agttttatcg ttggactgat catcgatgaa
     3301 aatgaccgtt tttactttgt gcaaaaggat ggtcaaacct atgctcttgc taaggaagaa
     3361 ggtcaacata cagtagggga tacggtcaaa ggttttgcat acacggatat gaagcaaaaa
     3421 ctccgcctga caaccttaga agtgactgcc actcaggacc aatttggttg gggacgtgtc
     3481 acagaggttc gtaaggattt gggtgtcttt gtggatacag gccttcctga caaggaaatc
     3541 gttgtgtcac tcgatattct ccctgagctc aaggaactct ggcctaagaa gggcgaccaa
     3601 ctctacatcc gtcttgaagt ggataagaaa gaccgtatct ggggcctctt ggcttatcaa
     3661 gaagacttcc aacgtcttgc tcgtcctgcc tacaacaaca tgcagaacca aaactggcca
     3721 gccattgttt accgtctcaa gctgtcagga acttttgttt acctaccaga aaataatatg
     3781 cttggtttta ttcatcctag cgagcgttac gcagagccac gtttggggca agtattagat
     3841 gcgcgcgtta ttggtttccg tgaagtggac cgcactctga acctctccct caaaccacgc
     3901 tcctttgaaa tgttggaaaa cgatgctcag atgattttga cttatttgga aagtaatggc
     3961 ggtttcatga ccttaaatga taagtcatct ccagacgaca tcaaggcaac ctttggcatt
     4021 tctaaaggtc agttcaagaa agctttaggt ggtcttatga aggctggtaa aatcaagcag
     4081 gaccagtttg ggacagagtt gatttaggga ggcttatgag aaaatcattt tacacttggc
     4141 tcatgaccga gcgcaatcct aaaagtaaca gtcccaaagc aattttggca gacctcgctt
     4201 ttgaagaggc agcttttcca aaacatacag atgattttga tgaggtcagt cgctttttgg
     4261 aggagcatgc cagtttctct tttaacctag gagattttga cagcatttgg caggaatatc
     4321 tagaacacta gcatttattc attgggtttg ggctagtaat ttctccatcc ctctgctata
     4381 ataaaaagaa ataaaaggat tagagaggtt ctttatttga aggaacattc aatagacatt
     4441 caactgagtc atccagatga cctgtttcat ctttttggtt ccaatgaacg ccatcttcgt
     4501 ttgatggaag aagagcttga tgttgtgatt catgctcgta cggagattgt ccaggttttg
     4561 ggagaagagt ctgcctgtga ggaagcccgt caagctattc aggctttgat ggtcttggta
     4621 aatcgtggga tgaccgttgg tacgccagat gtagtcactg cgattagcat ggtcaaaaat
     4681 gatgaaattg acaagtttgt cgccctttac gaagaagaaa ttatcaaaga taatactggg
     4741 aaacctatcc gtgtcaaaac cctagggcaa aagctttatg tggacagtgt caaacagcat
     4801 gatgtgacct ttggaattgg gccagcaggt acagggaaga ccttccttgc agtgaccttg
     4861 gcagtgactg cccttaaacg tgggcaagtc aagcgaatta tcctaactcg tccagcggtg
     4921 gaagcgggag agagtcttgg atttcttccg ggtgatctta aggagaaggt ggatccttac
     4981 cttcgtcctg tttacgatgc cttgtatcaa attcttggga aagaccaaac gactcgtctc
     5041 atggagcgtg aaattatcga aattgcgccc cttgcctata tgcgtggccg gaccttggat
     5101 gatgcctttg tcattctcga tgaggcgcaa aacacgacca tcatgcagat gaagatgttc
     5161 ttgacgcgtt taggctttca ttctaagatg attgtcaatg gagatatcag tcagattgac
     5221 ctgccacgta atgtcaagtc cggtttgatt gatgctcaag agaaactcaa gaacatccat
     5281 cagattgact ttgttcattt ttcagccaag gatgtggttc gccatccagt tgtcgctcag
     5341 attatccgag cctatgaata ttctactgaa gttgcacacg actgattttg aggaagttcg
     5401 cctgcaaaag aatatacttt tgagagagga aaatccagtt gtataggcta aaggttttat
     5461 ccaaaggtct gagacaacga ttaggcacga tggaaagaac ttttatgtgg ctgatgacga
     5521 tcagtgcatc ttcctgtgtc ataatcacag ggcacaagaa agtaggaatt tgaaaagatg
     5581 attgaccaac tatctaagta ttacagttgt aggatactaa ctgaaaagga tattccaagt
     5641 attttatctt tatatgaaag taatcctctg tattttcagc attgtccacc agagccaaat
     5701 tttgcaactg taaaagagga catgctttgt ctacctgaag gtaaagctaa ggctgataag
     5761 ttttttgttg gattttggaa tggatctgac cttgtggctg ttatggattt tgtctatgcc
     5821 tatcctgatg aggagactgt ttttattggt ttgtttatgg ttgatcaagc ctatcagagg
     5881 aaagggattg gtagtcatat tgtgacagaa gcactagctt attttgctaa gaactttcga
     5941 aaggcacgtt tggcttatgt taagggaaat ccgcaatctc agcatttttg ggaaaagcag
     6001 ggttttaaat caattggatg cgaggttaag caagaactct atacggttgt tatcgctgaa
     6061 cagagcctag aagattagaa atggcatcaa gtaagaacta tttggaattt gttttggaac
     6121 aattatcagg attagatgat gtgacttacc gttccatgat gggggagtat attctttact
     6181 tccgcggcaa gattattggc ggcatttatg atgatcgctt tttagttaaa cccgtgcaag
     6241 cagtcttaga taagattgac caatcttctt ttgagtttcc atacaaaggt gccaaagaaa
     6301 tgatttgagt ggaagaactt gataataaga tgtttctata agacctgatt ttagctatgt
     6361 ataaccaact gccaacgccc aaacctaaaa agaaaaagca agggtgaacg aagtgaaaaa
     6421 gaagtctgct aaggccctgt ctttgcacgg gtaaaatttt atatataaaa agaagctggg
     6481 actaaagagc tcagcttcct ttggtttata taattgtcat tacaagacga agtggttggg
     6541 cgaaactctg ttgactttat tcaatttaga gtttcttatg cacaattgag tctggaacga
     6601 aagtctccag ttgcaaagta tacagtacaa taaaccaacg atgtaatagc tgatgacaca
     6661 aagcacagtg ggtaggactt gcgaagtcac ccttttcttt tcaaaattta tactaaatca
     6721 ttgatatcag tgtagtcacg attaagtcct tgagcaactg gtaggctagt caagtaacct
     6781 tgataagtgg tcacaccttg acgcaagcct tcatcttcag agattgcttg tgcgaatcct
     6841 ttgccagcca aagcttcgat ataaggaaga gtgacattgg ttagggcgat ggttgaagtg
     6901 cgggcaaccg caccagggat attggcaacg gcatagtgga gaacaccgtg tttttcatag
     6961 acgggttcat cgtgcgttgt cacacggtca gctgtttcga taacgccacc ttggtcaaca
     7021 gcaacgtcaa cgatacagag cctggacgca tttgtttgac catctcatct gtcaccaatt
     7081 ccggtgcttt tgcaccaggg atgagaatgg ctccaatcac cacatcagca tctctcacac
     7141 ttgcttcaat gttgaatgaa ttagacataa gagtttgaat ttgacttcca aagacttctt
     7201 ctagaactga gagacgcttg gcactaatat ctaaaatagt cacttgagca ccaagaccaa
     7261 gggcgatgcg ggcagcatgt gtaccgacga caccaccacc gatgatagtt acttttcctt
     7321 ttggaacacc tggtacacca ccaagtagaa caccagagcc accagcttgc ttagtaagga
     7381 agtgagctcc gatttaaaca gccatacgac ctgcaacctc actcatagga acgaggagcg
     7441 gtagttgtcc ttgattgtca cgaacagttt cataggcaat tcctgttgtt tttgctgcta
     7501 acatagcatc tgctaattct ggagcagcgg ccatgtgcaa gtaggtgaag agaagaagat
     7561 cgtcgcgcaa gtaaccgtat tcagaactta aaggttcttt tactttcaca accaactctg
     7621 ctgcccaagc ttcaccagca gtagcgacaa tctcagctcc ttgcttttga tagtcagcat
     7681 cagtaaagcc agaaccgaga ccagcatttg tttcgataag gacacgatga ccacgactaa
     7741 ctaagctatg aacacctgca ggtgtgaggg cgacacagtt ttcgttattt ttaatttctt
     7801 ttgggattcc gattaacatt gagataacct acctttcaat tgacggtctt gttttggttg
     7861 tcacattcca gttcataaat caaaaatgtg acggtttcat tgtatatgaa accgcttcaa
     7921 aaatcaagaa aaacttgtca tccaaatttt ttatgctaga ctagtgaaaa tcaagctttg
     7981 attggttgga aaagtatgga atcaattttt gtaaaacttg cccagtatcc gtctatagaa
     8041 acggagcgtt tattgcttag acctgtaact ttggatgatg cggaagcaat gtttgactat
     8101 gcctcggaca agggtaatac acgttacact tttccaacca atcaaagctt ggaagaaacc
     8161 aagaataaca ttgctcagtt ctatttggct aatcccttgg gacgttgggg aatagaacta
     8221 aaaagcaatg gtcagtttat tggaaccatt gacttgcaca agattgattc tgttcttaag
     8281 aaggcagcta ttggctacat tatcaataaa aagtattgga atcaaggatt aacgacagaa
     8341 gccaatcgtg ctgtgattga gctagctttt gagaagatag ggatgaataa gttgactgcc
     8401 cttcacgata aggacaatcc tgcgtcagga aaggtcatgg agaaatcagg catgcgtttt
     8461 tcccatgcag aaccatatgc ttgtatggac cagcatgaaa aaggccgaat cgtgacaaga
     8521 gttcattatg tcttgaccaa ggaagactat tttgcaaata aataagcagt tgaaaagaaa
     8581 tttttcgact gttttttctt cctcttacga ataatctaag agaggagaaa atatggaagc
     8641 aattatcgag aaaatcaaag agtataaaat catcgtcatc tgtactggtc tgggcttgct
     8701 tgtaggcgga tttttcctgc taaagccagc tccacaaaca cctgtaaagg aaacgaattt
     8761 gcaggctgaa gttgcagctg tttccaagga tttggtatcc gaaaaggaag tgaacaagga
     8821 agagaaggaa gaaccccttg aacaagatct aatcacagta gatgtcaaag gtgctgtcaa
     8881 atcgccaggg atttatgact tgcctgtagg tagtcgagtc aatgatgctg ttcagaaggc
     8941 tggtggcttg acagagcaag cagacagcaa gtcgctcaat ctagctcaga aagttagtga
     9001 tgaggctctg gtttacgttc ctactaaggg agaagaagca gttagtcaac agactggttc
     9061 ggggacagct tcttcaacaa gcaaggaaaa gaaggtcaat ctcaacaagg ccagtctgga
     9121 agagcttaaa caggttaagg gactgggagg aaaacgagct caggacatta tagaccatcg
     9181 tgaggcaaat gggaaattca agtcgattga tgaactcaag aaggtctctg gcattggtgc
     9241 taagaccata gaaaagctaa aagactatgt tacagtggat taagaacatc cccattcccc
     9301 taatttacct gagttttttg ttactttggt tttattacgc tattttctca gcatcctatc
     9361 ttgctttgtt gggctttgtt tttctgctag tctgtctctt tttccaattt ccttggaaat
     9421 ctgctagcaa agttctagtg atttgtggaa tctttggatt ttggtttgtt tttcaaaatt
     9481 ggcaacagag tcaagcgagt caaaatctgg cggattctgt tgaaggggta cggattttgc
     9541 ctgatactat taaggttaat ggtgatagtc tatcctttcg tggcaagtct aacggtcgtg
     9601 ctttccaagt ctattataaa ctccagtccg aggaggagaa agaagccttt caagctttaa
     9661 ccgacctgca tgagatagga ctagaaggga agctttcgga gccagaaggg cagagaaatt
     9721 ttggtggctt taattaccaa gcctatctga agactcaggg aatttaccag actctcaata
     9781 tcaaaacaat ccagtcactt caaaagattg gcagttggga tataggagaa aacttgtcca
     9841 gtttacgtcg aaaggctgtg gtttggatta agacgcactt tccagaccct atgcgcaatt
     9901 acatgacagg actcttgctg ggacatctgg acaccgactt tgaggagatg aatgagcttt
     9961 attccagtct aggaattatc cacctctttg ccctatctgg catgcaggta ggttttttca
    10021 tgaatggatt taagaaactt ctcttgcgat tgggcttgac ccaagaaaag ttgaaatggc
    10081 tgacttatcc cttttccctt atctatgcgg gactaactgg attttcagca tcggttattc
    10141 gcagtctctt gcaaaagcta ctggctcaac atggggttaa gggcttggat aattttgcct
    10201 tgacggtgct tgtcctcttt attgtcatgc caaacttttt cttgacagca ggaggagtct
    10261 tgtcctgcgc ttatgctttt atcctgacca tgaccagcaa agaaggggag gggctcaagg
    10321 ctgttactag tgaaagtcta gtcatctcct tgggcatatt gcccattcta tccttctatt
    10381 ttgcggaatt tcaaccttgg tctatccttt tgacctttgt cttttccttt ctatttgact
    10441 tgaccttctt accgctcttg tctattttat ttgtcctttc ctttctctat ccagtcattc
    10501 agctgaactt tatctttgaa tggttagagg gcattattcg cttggtctcg caggtggcaa
    10561 ggagaccgct tgtctttggt caacccaacg catggctttt aatcttattg ttaatttcct
    10621 tggctttggt ctatgatttg aggaaaaaca ttaaaggatt aacagtattg agtttattga
    10681 ttacaggtct ctttttcctt accaagtatc cactggaaaa tgaaatcacc atgctggatg
    10741 tggggcaagg agaaagtatt ttcctacggg atgtaactgg gaaaaccatt ctcatagatg
    10801 taggtggtaa ggcagaatct tataagaaaa tcgaaaaatg gcaagaaaag atgacgacca
    10861 gcaatgcaca gagaagcttg ataccctatc ttaaaagtcg aggagtagct aagattgacc
    10921 agctgatttt gacaaatacg gacaaggaac atgtcggaga tttgttagag gtgaccaagg
    10981 ctttccatgt aggggagatt ttagtatcaa aaggcagttt gaagcagaag caatttgtgg
    11041 tagaactaca ggcgactcaa acaaaggtgc gtagtatgac agtaggggag aacttgccca
    11101 tttttggaag tcagttagaa gttctatctc taaggaaaat gggagatgga ggacacgatg
    11161 ataccctagt tctgtatggg aaattcttgg ataagcaatt tctcttcacg ggaaatttgg
    11221 aggagaaagg agagaaggac ttgctgaagc actatccaga cttgaaagta aatgttttga
    11281 aagctagcca acatggcaat aaaaaatcat caagtccaac ctttctagaa aaactcaaac
    11341 cagagcttac tcttatctca gttggaaaga gcaatcgaat gaaactcccc catcaggaaa
    11401 cattgacacg actggaaggt atcaatagca aagtttatcg aactgaccag caaggagcta
    11461 tacgttttaa ggggttggat agttggaaaa tcgaaagtgt tcgataggaa agataaatgt
    11521 tgtagattag tgaaataaac taaaaatttg ttgcataata atgataaaaa tggtataatg
    11581 aaaacgtatt caatattgag gatataaaat cattaaaaat cagcaaaagt tattttatta
    11641 gttagtttat aatctattgg tcttcttcag tccagtgtat ctgctaagag tgttcttaaa
    11701 agtatcggta tgattggaat atcttctata agtctagtat gaatttccat ggatatagac
    11761 atactaacgt tccgtcatgg tctcgttatt acagctattc tgagtatcca gttggttctg
    11821 gctggaacta cggtcgttat gagatcataa actattacag cggaggctat taattcttaa
    11881 agagtgagaa aaaggagggc tagatatgtt gcagcttact catgtgacct taaaaacgcg
    11941 acaagtcatc ttgcaagatg cggatttcac ctttaaaaag ggtaggtttt atggtcttct
    12001 tgctatcaat ggctctggaa agacgaccct gttccgtgcc attagcaatt taattcccat
    12061 aagtagtgga aatatcgcag ctcctccttc tttattttat tatgagagta ttgaatggct
    12121 ggatggaaac ttaagtggga tggactacct tcgtcttatc aaaaacatct ggaagtcaga
    12181 cctaaacttg agagatgaaa tcgcctactg ggaaatggct gactatatca gtcttcccat
    12241 ccgcaagtat tccttaggca tgaagcaacg cttggtgatt gctatgtatt ttctcagtca
    12301 ggccaaatgc tggctcatgg atgagattac aaatggctta gatgagtatt atcgacagaa
    12361 gttttttgat aggttagcac aaatcgatag acaagaacag ctggttcttt taagttccca
    12421 ctataaggaa gagttggttg atatctgcga tagagtagta accattcatc aggggcagat
    12481 agaagaggtt tagtttatga aagatgttag tctattttta ttgaaaaaag ttttcaaaag
    12541 ccgcttaaac tggattatct tagctttatt tgtatctgta ctcggtgtta ctttttattt
    12601 taatagtcag actgcaaact cagtcagctt ggagagagag ttggaaactc gtcttgtaga
    12661 ccgtgagaga gtcatcaatg aatatgaaga gaaactctcc caaatgtctg ataccagctc
    12721 ggaggaatac cagtttgcta aaaataattt agacgtgcaa aaaaatcttt tgacgcgaaa
    12781 gacagaaatt ctaactttac taaaagaagg gcgctggaaa gaggcctact atctgcagtg
    12841 gcaagatgaa gagaaagatt atgaatttgt atcaaatgac ccgactgcta gctctgaatt
    12901 aaaaatgggg gttgaccgcg aacggaagat ttatcaagcc ctgtatccct tgaacataaa
    12961 agcacatact ttggagtttc cgacccacgg gattgatcag attgtctgga ttttagaggc
    13021 tatcatccca accttatttg tgattggtat tatttttatg ctaacacaac tatttgcaga
    13081 aagatatcaa aatcatctgg acacagctca cttatatcct gtttcaaaag tgacatttgc
    13141 aatatcctct cttggagttg gagtgggata tgtaactgtg ctgtttatcg gaatctgtgg
    13201 cttttctttt ctagtgggaa gtctgataag tggttttgga cagttagatt atccctactc
    13261 aatttatagc ttagtgaatc aagaagtaac tattgggaaa atacaagatg tattatttcc
    13321 tggcttactc ttagctttct tagcctttat cgtcattgtg gaagttgtgt acttgattgc
    13381 ttattttttc aagcaaaaaa tgcctgtcct ctttctttca ctcattggga ttgttggctt
    13441 attgtttggc atccaaacaa ttcagcctct tcaaaggatt gcacatctga ttccctttac
    13501 ttacttgcgt tcagtggaga ttttatctgg aagattatct aagcagattg ataatgtcga
    13561 tctaaattgg agcatgggga tggtcttact tccttgcctg attatccttt tgttagtggg
    13621 gattctattt atcgaaagct ggggaagttc acgtaaaaag gaagttttta aagtttagtt
    13681 ttcctatagg gaagctaaag aaaaactaac atagggagag aatcaacttg gttctctctt
    13741 tttgattcga aaaccaaacc aaaatacaaa cacaaacttt tcaaaaaata actttttatc
    13801 ttgacaagag ctagaaaact tggtatcata taaaagttga gaaaagcaga agtgagagct
    13861 tctcgccttg tgacattaag ttgcctggcc ctacggatga aaagtttcga agaaacgcta
    13921 tcataacgtg cgggcttgta tatttacgag tccgctattg tttttctcta ataaaacaaa
    13981 agaggtgaaa accatagcaa agcaagactt attcatcaat gatgagattc gtgtacgtga
    14041 agttcgcttg attggtcttg agggagaaca gctaggcatc aagccactca gtgaagcgca
    14101 agctttggct gataacgcta atgttgacct agtattgatt caaccccaag ccaaaccgcc
    14161 tgttgcaaaa attatggact acggtaagtt caaatttgag taccagaaga agcaaaaaga
    14221 acaacgtaaa aaacaaagcg ttgttactgt gaaagaagtt cgtctaagtc caacaattga
    14281 caagggtgac tttgatacaa aacttcgtaa tgcacgtaag ttccttgaaa aaggaaataa
    14341 agttaaggta tctattcgct ttaagggtcg tatgattacc cataaagaga ttggtgcaaa
    14401 agttttagcc gagtttgctg aagcaactca agatattgcc atcatcgaac aacgtgctaa
    14461 aatggatgga cgtcaaatgt tcatgcaatt ggcgccagcg actgacaaaa aataattgtc
    14521 agaaagttaa ataaaggaga aaaaatcatg ccaaaacaaa aaacacaccg cgcatcagct
    14581 aaacgtttca aacgtacagg ttctggtgga cttaaacgtt tccgtgctta cacttctcac
    14641 cgtttccacg gaaaaactaa gaaacaacgt cgtcatcttc gtaaagcatc tatggtgcat
    14701 tcaggagatt acaaacgtat caaagcaatg cttactcgct tgaaataata gcttgactgt
    14761 aagtaaacaa ttctaggaaa tatttggagg aaataaaaca tggcacgtgt taaaggtggc
    14821 gttgtatcac gcaaacgtcg taaacgtatt cttaaattag caaaaggtta ctatggagct
    14881 aaacacatct tgttccgtac tgcaaaagaa caagtaatga actcttacta ctatgcatac
    14941 cgtgaccgtc gtcagaaaaa acgtgacttc cgcaaattgt ggattactcg tatcaatgcg
    15001 gcagctcgta tgaacggact ttcatactca caattgatgc atggtttgaa attggctgag
    15061 atcgaagtta accgtaaaat gcttgctgac ttggctgtta acgatgcagt agctttcaca
    15121 gctcttgcag atgcagctaa agcaaaactt ggtaaataac aactgatgag actggaacag
    15181 gattgtccag tctttttaaa aataaaggag aaagatatgg cttcaaaaat gctacacact
    15241 tgcttacgag tagaaaatct tgaaaaatca atcgcatttt atcaagatgc ttttggtttt
    15301 aaagaattgc gtcgcagaga ttttccagac catgccttca cgattgtcta tctaggtctt
    15361 gagggtgatg attatgagtt ggagttgact tataactacg atcacggtcc gtatgtggtt
    15421 ggtgatgggt ttgcccatat cgccctcagt acacctgatc ttgaggcact tcatcaagaa
    15481 cacagtgcaa aaggctatga agtgactgag ccaaatggtc taccaggaac tacaccaaac
    15541 tattactttg ttaaagaccc tgatggatat aaggtcgaag tcattcgtga aaaataattt
    15601 cgtgcaagtt aagtagaata ttcgttttct acttgacttt ttttagctga aagagtatac
    15661 taataaaaat tcaaccttta aggggagtcc agagagactc acaaggtgtc agataaaaga
    15721 atagtgcaat tttctagagg agaccttttg agtgtgctct cttgtgttgt acgattttaa
    15781 ctgaggcctt gcactagcaa ggtcttttct ttatctggtc cccttaaaat ttaaggagga
    15841 aaagttatga atctcacatg taagaagcgt ttgggtgtca ttcggttgga aaccatgaag
    15901 gtggttgcac aagaggaaat cgcgccagca atctttgaat tagtcctaga aggagaaatg
    15961 gttgaagcca tgcgagcagg ccaatttctt catctgcgtg taccagacga tgcccatctc
    16021 ttacgtcgtc ctatttcaat ttcgtctatt gacaaggcaa acaagcagtg tcacctcatt
    16081 tatcggattg agggagctgg gactgcaatt ttttcaacct taagtcaggg agacactctt
    16141 gatgtgatgg ggcctcaggg aaatggtttt gacttgtctg accttgatga gcagaatcag
    16201 gttctccttg ttggtggtgg gattggtgtt ccacccttgc ttgaggtggc caaggaattg
    16261 catgaacgtg gagtgaaagt agtgacagtc ctcggttttg ctaataagga tgctgttatt
    16321 ttgaaaacgg aattggctca gtatggtcag gtctttgtaa cgacagatga tggttcttat
    16381 ggcatcaagg gaaatgtttc cgttgttatc aatgatttag atagtcagtt tgatgctgtt
    16441 tactcgtgtg gggctccagg aatgatgaag tatatcaatc aaacctttga tgatcaccca
    16501 agagcctatt tatctctgga atctcgtatg gcttgtggga tgggagcttg ctatgcctgt
    16561 gttctaaaag taccagaaag cgagacggtc agccaacgcg tctgtgaaga tggtcctgtt
    16621 ttccgcacag gaacagttgt attataagga gaaaattatg actacaaatc gattacaagt
    16681 gtctctacct ggtttggatt tgaaaaatcc gattattcca gcatcaggct gttttggctt
    16741 tggacaagag tatgccaagt actatgattt agacctttta ggttctatta tgatcaaggc
    16801 gacaaccctt gaaccacgtt ttgggaatcc aactccaaga gtggcagaga cgcctgctgg
    16861 tatgctcaat gcaattggct tgcaaaatcc tggtttagag gttgttttgg ctgaaaagct
    16921 accttggctg gaaagagaat atccaaatct tcctattatt gccaatgtag ctggtttttc
    16981 aaaacaagag tatgcagctg tttctcatgg gatttccaag gcaactaatg taaaagctat
    17041 cgagctcaat atttcttgtc ccaatgttga ccactgtaat catggacttt tgattggtca
    17101 agatccagat ttggcttatg atgtggtgaa agcagctgtg gaagcctcag aagtgccagt
    17161 ttatgtcaaa ttaaccccga gtgtgaccga tatcgttact gtcgcaaaag ctgcagaata
    17221 tgcgggagca agtggcttga ccatgatcaa tactctggtt ggaatgcgct ttgacctcaa
    17281 aaccagaaaa ccaatcttgg ccaatggaac aggtggaatg tcaggtccag cagtctttcc
    17341 agtagccctc aaactcatcc gccaagtagc ccaaacaaca gacctgccta tcattggaat
    17401 ggggggagtg gattcggctg aagctgccct agaaatgtat ctggctgggg catctgctat
    17461 cggagttgga acagctaact ttaccaatcc ttatgcctgc cctgacatca tcgaaaattt
    17521 accaaaagtc atggataaat acggtattag cagtctggaa gaactccgtc aggaagtaaa
    17581 agagtctctg aggtaaactg caatcaatct gttcttgatt ttttattagt ttgtaatatg
    17641 aatttaggag aattttggta caataaaata aataagaaca gaggaagaag gttaatgaag
    17701 aaagtaagat ttattttttt agctctgcta tttttcttag ctagtccaga gggtgcaatg
    17761 gctagtgatg gtacttggca aggaaaacag tatctgaaag aagatggcag tcaagcagca
    17821 aatgagtggg tttttgatac tcattatcaa tcttggttct atataaaagc agatgctaac
    17881 tatgctgaaa atgaatggct aaagcaaggt gacgactatt tttacctcaa atctggtggc
    17941 tatatggcca aatcagaatg ggtagaagac aagggagcct tttattatct tgatcaagat
    18001 ggaaagatga aaagaaatgc ttgggtagga acttcctatg ttggtgcaac aggtgccaaa
    18061 gtaatagaag actgggtcta tgattctcaa tacgatgctt ggttttatat caaagcagat
    18121 ggacagcacg cagagaaaga atggctccaa attaaaggga aggactatta tttcaaatcc
    18181 ggtggttatc tactgacaag tcagtggatt aatcaagctt atgtgaatgc tagtggtgcc
    18241 aaagtacagc aaggttggct ttttgacaaa caataccaat cttggtttta catcaaagaa
    18301 aatggaaact atgctgataa agaatggatt ttcgagaatg gtcactatta ttatctaaaa
    18361 tccggtggct acatggcagc caatgaatgg atttgggata aggaatcttg gttttatctc
    18421 aaatttgatg ggaaaatggc tgaaaaagaa tgggtctacg attctcatag tcaagcttgg
    18481 tactacttca aatccggtgg ttacatgaca gccaatgaat ggatttggga taaggaatct
    18541 tggttttacc tcaagtctga tggtaaaatg gctgaaaaag aatgggtcta cgattctcat
    18601 agtcaagcct ggtactactt caaatctggt ggctacatgg cgaaaaatga gacagtagat
    18661 ggttatcagc ttggaagcga tggtaaatgg cttggaggaa aaactacaaa tgaaaatgct
    18721 gcttactatc aagtagtgcc tgttacagcc aatgtttatg attcagatgg tgaaaagctt
    18781 tcctatatat cgcaaggtag tgtcgtatgg ctagataagg atagaaaaag tgatgacaag
    18841 cgcttggcta ttactatttc tggtttgtca ggctatatga aaacagaaga tttacaagcg
    18901 ctagatgcta gtaaggactt tatcccttat tatgagagtg atggccaccg tttttatcac
    18961 tatgtggctc agaatgctag tatcccagta gcttctcatc tttctgatat ggaagtaggc
    19021 aagaaatatt attcggcaga tggcctgcat tttgatggtt ttaagcttga gaatcccttc
    19081 cttttcaaag atttaacaga ggctacaaac tacagtgctg aagaattgga taaggtattt
    19141 agtttgctaa acattaacaa tagccttttg gagaacaagg gcgctacttt taaggaagcc
    19201 gaagaacatt accatatcaa tgctctttat ctccttgccc atagtgccct agaaagtaac
    19261 tggggaagaa gtaaaattgc caaagataag aataatttct ttggcattac agcctatgat
    19321 acgacccctt acctttctgc taagacattt gatgatgtgg ataagggaat tttaggtgca
    19381 accaagtgga ttaaggaaaa ttatatcgat aggggaagaa ctttccttgg aaacaaggct
    19441 tctggtatga atgtggaata tgcttcagac ccttattggg gcgaaaaaat tgctagtgtg
    19501 atgatgaaaa tcaatgagaa gctaggtggc aaagattagt actataagtg aatatgattt
    19561 gagtgaatag taagttaaaa atcctgattt caagtaaaat caggattttt tcatggatgc
    19621 aatttttttg gagtctggtg tgacgcggag ggtcttttgt cctgtgtaag tgacaaagcc
    19681 gggttttcca ccagttggtt tattgagttt tttgacttca atcatatcta cctgcaccag
    19741 attcgacagg cgcccttgag agaagtaggc agctaactct gctgcgtctg tcttgactgc
    19801 atcagatggg tcaagatttc ctgagatgac aacatggctt ccaggaatgt ccttagcatg
    19861 gaaccaaagt tcctccttgc gggccatttt aaaggtcaat tcctcatttt gaagattgtt
    19921 tcgtccgaca tagatgatgg ttttgccatc gcttgctaga tattgttcta gttttttgcg
    19981 tttctggatt ttctcccgtt gtcttctgcg gataaaacct gtttgaatca attcttcacg
    20041 gatttcagcg atttcttcca gtccagcttg gttgaggacg gtttctacac tttccagata
    20101 gagaatagtg gctttggttt cttcaatcaa atcagtcaag tatttgacag cttctttgag
    20161 tttctgatac cgtttaaaat agcgttgggc attctggttg ggagtcagag ccttatcaag
    20221 cgcaatcatg ataggttggt tggtatagta gttgtctagg ataacctggt cttggtcgtt
    20281 aggcacttgg tggaggaagg ttgtcagcaa ttctcctttt tgacgaaatt cttcagcgtt
    20341 gtctgtcgcc agtaactctt tttcctgttt tttgagtttg tgtcggtttt tctgaagttc
    20401 attttcaaca cgacgaatca gttcactggc ctgctgtttg acgcggtcgc gctcagcctt
    20461 atccttatag taggtgtcca acaaatcaga aagatttgca aaaggctctc ccacctgatt
    20521 tgcaaaagga actggactga aggaagtctc agtcaagcat ggcttggttt cttgattgaa
    20581 aaaatttcgg aaagcggaaa gtttttcact aaccagtatc ctttccaatt catttgccgt
    20641 atcgcgtccc agaccttgaa agaggctttg aagatttttt gctgttagtt cttgggtttg
    20701 caggatttca aagagctttt catccttgat agtaaaagga ttgagagatt ttgtacttgg
    20761 cggagcgata taggtcgatc ctggaagtaa ggtgcggtag ctattttgtg aaaagccgac
    20821 gtgtttgata acttcgagga ttttatgact gcttttatcg accagtagaa tattactgtg
    20881 tttccccata atttcgataa tcaaggtagc ctggatatgg tctccaatct cgtttttatt
    20941 ggaaactgta atttccacaa tacggtcatt ttccacttgc tcaatcgact caatcagggc
    21001 cccctgcaaa tactttctca aaaccatgat aaaggtagaa ggttgagctg gattttcaaa
    21061 agtcgtttgg gtcagctgaa tgcgtccaaa aactggatgg gcagaaagga gcaggcgatg
    21121 gctttggcga ttgctgcgga tttgcaagac caactcttgt tcaaaaggct gattgatttt
    21181 ctggatgcga ccattcacta attcgcttcg caattcctca actatgtggt gtaaaaaaaa
    21241 tccgtcaaat gacatcgttc tctccttgtg attgtattcc atagtattat atcaaaaagg
    21301 tagaataaaa tcatggaaat gtggtataat aaagccaagt aaagagaaac gagaagcaca
    21361 tgtatattga aatggtagat gaaactggtc aagtttcaaa agaaatgttg caacaaaccc
    21421 aagaaatttt ggaatttgca gccaaaaaat taggaaaaga agacaaggag atggcagtca
    21481 cttttgtgac caatgagcgt agtcatgaac ttaatctgga gtaccgtgac accgaccgtc
    21541 cgacagatgt catcagcctt gagtataaac cagaattgga aattgccttt gacgaagagg
    21601 atttgcttga aaatccagaa ttggcagaga tgatgtctga gtttgatgcc tatattgggg
    21661 aattgttcat ctctatcgat aaggctcatg agcaggccga agaatatggt cacagctttg
    21721 agcgtgagat gggcttcttg gcagtacacg gctttttaca tattaacggc tatgatcact
    21781 atactccgga agaagaagcg gagatgttcg gtttacaaga agaaattttg acagcctatg
    21841 gactcacaag acaataaacg aaaatggaaa aatcgtgacc tgatatccag tttagaattt
    21901 gccatcacag ggatttttac tgctatcaag gaagaacgca atatgcgaaa acatgcagtg
    21961 acggctctag tggtcatcct tgcaggtttt ggttttcagg tgtcacgaat cgaatggctc
    22021 tttctcctat tgagcatttt cttggtggta gcctttgaaa ttatcaattc tgctattgaa
    22081 aatgtggtgg atttggccag tcactatcac ttttccatgc tggctaaaaa tgccaaggat
    22141 atggcggctg gcgcggtatt agtggtttct ctttttgcag ccttaacagg cgcattgatt
    22201 tttctcccac gaatctggga tttattattt taaacagtaa gaggaaatta tgacttttaa
    22261 atcaggcttt gtagccattt taggacgtcc caatgttggg aagtcaacct ttttaaatca
    22321 cgttatgggg caaaagattg ccatcatgag tgacaaggcg cagacaacgc gcaataaaat
    22381 catgggaatt tacacgactg ataaggagca aattgtcttt atcgacacac cagggattca
    22441 caaacctaaa acagctctcg gagatttcat ggttgagtct gcctacagta cccttcgcga
    22501 agtggacact gttcttttca tggtgcctgc tgatgaagcg cgtggtaagg gggacgatat
    22561 gattatcgag cgtctcaagg ctgccaaggt tcctgtgatt ttggtggtga ataaaatcga
    22621 taaggtccat ccagaccagc tcttgtctca gattgatgac ttccgtaatc aaatggactt
    22681 taaggaaatt gttccaatct cagcccttca gggaaataac gtgtctcgtc tagtggatat
    22741 tttgagtgaa aatctggatg aaggtttcca atatttcccg tctgatcaaa tcacagacca
    22801 tccagaacgt ttcttggttt cagaaatggt tcgcgagaaa gtcttgcact taactcgtga
    22861 agagattccg cattctgtag cagtagttgt tgactctatg aaacgagacg aagagacaga
    22921 caaggttcac atccgtgcaa ccatcatggt cgagcgcgat agccaaaaag ggattatcat
    22981 cggtaaaggt ggcgctatgc ttaagaaaat cggtagcatg gcccgtcgtg atatcgaact
    23041 catgctagga gacaaggtct tcctagaaac ctgggtcaag gtcaagaaaa actggcgcga
    23101 taaaaagcta gatttggctg actttggcta taatgaaaga gaatactaag tagaggtagg
    23161 ctcatgcctg cttcttgttt ttacagaagg aggacttatg cctgaattac ctgaggttga
    23221 aaccgtttgt cgtggcttag aaaaattgat tataggaaag aagatttcga gtatagaaat
    23281 tcgctacccc aagatgatta agacggattt ggaagagttt caaagggaat tgcctagtca
    23341 gattatcgag tcaatgggac gtcgtggaaa atatttgctt ttttatctga cagacaaggt
    23401 cttgatttcc catttgcgga tggagggcaa gtatttttac tatccagacc aaggacctga
    23461 acgcaagcat gcccatgttt tctttcattt tgaagatggt ggcacgcttg tttatgagga
    23521 tgttcgcaag tttggaacca tggaactctt ggtgcctgac cttttagaca cctactttat
    23581 ttctaaaaaa ttaggtcctg aaccaagcga acaagacttt gatttacagg tctttcaatc
    23641 tgcccttgcc aagtccaaaa agcctatcaa atcccatctc ctagaccaga ccttggtagc
    23701 tggacttggg aatatctatg tggatgaggt tctctggcga gctcaggttc atccagctag
    23761 accttcccag actttgacag cagaagaagc gactgccatt catgaccaga ccattgctgt
    23821 tttgggccag gctgttgaaa aaggtggctc caccattcgg acttatacca atgcctttgg
    23881 ggaagatgga agcatgcagg actttcatca ggtctatgat aagactggtc aagaatgtgt
    23941 acgctgtggt accatcattg agaaaattca actaggtgga cgtggaaccc acttttgtcc
    24001 aaactgtcaa aggagggact gatgggaaaa atcatcggaa tcactggggg aattgcctca
    24061 ggtaagtcaa ctgtgacaaa ttttctaaga cagcaaggct ttcaagcagt ggatgccgac
    24121 gcagtcgtcc accaactaca gaaacctggt ggtcgtctgt ttgaggctct agtacagcac
    24181 tttgggcaag aaatcattct tgaaaacgga gaactcaatc gccctctcct agctagtctc
    24241 atcttttcaa atcctgaaga gcaaaaatgg tctaatcaaa ttcaagggga gattatccgt
    24301 gaggaactgg ctactttgag agaacagttg gctcagacag aagagatttt cttcatggat
    24361 attcccctac tttttgagca ggactacagc gattggtttg ctgagacttg gttggtctat
    24421 gtggaccgag atgcccaagt agaacgctta atgaaaaggg accagttgtc caaagatgaa
    24481 gctgagtctc gtctggcagc ccagtggcct ttagaaaaaa agaaagattt ggccagccag
    24541 gttcttgata ataatggcaa tcagaaccag cttcttaatc aagtgcatat ccttcttgag
    24601 ggaggtaggc aagatgacag agattaactg gaaggataat ctgcgtattg cctggtttgg
    24661 taattttctg acaggagcca gtatttcttt ggttgtacct tttatgccca tcttcgtgga
    24721 aaatctaggt gtagggagtc agcaagtcgc tttttatgca ggcttagcaa tttctgtctc
    24781 tgctatttcc gcggcgctct tttctcctat ttggggtatt cttgctgaca aatacggccg
    24841 aaaacccatg atgattcggg caggtcttgc tatgactatc actatgggag gcttggcctt
    24901 tgtcccaaat atctattggt taatctttct tcgtttacta aacggtgtat ttgcaggttt
    24961 tgttcctaat gcaacggcac tgatagccag tcaggttcca aaggagaaat caggctctgc
    25021 cttaggtact ttgtctacag gcgtagttgc aggtactcta accggtccct ttattggtgg
    25081 ctttatcgca gaattatttg gcattcgtac agttttctta ctggttggta gttttctatt
    25141 tttagctgct attttgacta tttgctttat caaggaagat tttcaaccag tagccaagga
    25201 aaaggctatt ccaacaaagg aattatttac ctcggttaaa tatccctatc ttttgctcaa
    25261 tctcttttta accagttttg tcatccaatt ttcagctcaa tcgatcggcc ctattttggc
    25321 tctttatgta cgcgacttag ggcagacaga gaatcttctt tttgtctctg gtttgattgt
    25381 gtccagtatg ggcttttcca gtatgatgag tgcaggagtc atgggcaagc taggtgacaa
    25441 ggtgggcaat catcgtctct tggttgtcgc ccagttttat tcagtcatca tctatctcct
    25501 ctgtgccaat gcctctagcc cccttcaact aggactctat cgtttcctct ttggcttggg
    25561 aaccggtgcc ttgattcccg gggttaatgc cctactcagc aaaatgactc ccaaagccgg
    25621 catttcgagg atctttgcct tcaatcaggt attcttttat ctgggaggtg ttgttggtcc
    25681 catggcaggt tctgcagtag caggtcaatt tggctaccat gctgtctttt atgcgacaag
    25741 cctttgtgtt gcctttagtt gtctctttaa cctgattcaa tttcgaacat tattaaaagt
    25801 aaaggaaatc tagtgcgagt aaaaattaat ctcaaatgct cctcttgtga cagtatcaat
    25861 tacctaacca gtaaaaattc aaaaacccat ccagacaaga ttgaggtttt aaagtattgt
    25921 cccaaggaaa gaaaagtaac cctacatctt gaatctaagt agattttatg gtaaaataat
    25981 agggatttta aggagtttga tatgtataac ctattattaa ccattttatt agtattatct
    26041 gttgtgattg tgattgcaat tttcatgcaa ccaaccaaaa accaatccag caatgtattt
    26101 gatgccagtt caggtgattt gtttgaacgc agtaaagctc gcggttttga agctgtaatg
    26161 cagcgtttga cagggatttt agtctttttc tggctagcca ttgccttagc attgacggta
    26221 ttatcaagta gataagaaaa taatgggcag gactaggtct ttgcctcttt ttatttttaa
    26281 aggatgtttg agaaggtttt acagtaaaag aaaattaaaa aatctagaaa gaaaatatga
    26341 aagatagaat aaaagaatat ttacaagaca agggaaaggt gactgttaat gatttggctc
    26401 agactttggg aaaagacagt tccaaggatt ttcgtgagtt gattaaaacc ttgtccttaa
    26461 tggaaagaaa gcaccagatt cgctttgaag aagatggtag tctgacatta gaaattaaga
    26521 aaaaacatga gattaccctc aaggggattt ttcatgccca taaaaatggc tttggctttg
    26581 ttagtctgga aggcgaggag gacgaccttt ttgtagggaa aaatgatgtc aactatgcta
    26641 ttgatggtga taccgtcgag gtagtgatta agaaagtcgc tgaccgcaat aagggaacag
    26701 cagcagaagc caaaattatt gatatcctag aacacagttt gacaacagtt gtcgggcaaa
    26761 tcgttctgga tcaggaaaag cctaagtatg ctggctatat tcgttcaaaa aatcagaaaa
    26821 tcagtcaacc gatttatgtt aagaaaccag ccctaaagtt agaaggaaca gaagttctca
    26881 aggtctttat cgataaatac ccaagcaaga aacatgattt ctttgtcgcg agtgttctcg
    26941 atgtagtggg acactcaacg gatgtcggaa ttgatgttct tgaggtcttg gaatcaatgg
    27001 acattgtatc cgagtttcca gaagctgttg ttaaggaagc agaaagtgtg cctgatgctc
    27061 cgtctcaaaa ggatatggaa ggtcgtctgg atctaagaga tgaaattacc tttaccattg
    27121 acggtgcgga tgccaaggac ttggacgatg cagtgcatat caaggctctg aaaaatggca
    27181 atctagagct tggggttcac atcgcagatg tttcttatta tgtgaccgag gggtctgccc
    27241 ttgacaagga agcccttaac cgtgcgactt ctgtttacgt gacagaccga gtggtgccaa
    27301 tgcttccaga acgactatca aatggcatct gctctctcaa tccccaagtt gaccgcctga
    27361 cccagtctgc tattatggag attgataaac atggtcgtgt ggtcaactat accattacac
    27421 aaacagttat caagactagt tttcgtatga cctatagcga tgtcaatgat atcctagctg
    27481 gcgatgaaga aaagagaaaa gaatatcata aaattgtatc aagtatcgaa ctcatggcca
    27541 agcttcatga aactttagaa aacatgcgtg tgaaacgtgg agctctcaat tttgatacca
    27601 atgaagcgaa gattttagtg gataaacaag gtaagcctgt tgatatcgtt cttcggcagc
    27661 gtggtattgc cgagcggatg attgagtctt ttatgttgat ggctaatgaa acagttgccg
    27721 aacatttcag caagttggat ttgcctttta tctatcgaat tcacgaggag cctaaggctg
    27781 aaaaggttca gaagtttatt gattatgctt cgagttttgg cttgcgcatt tatgggactg
    27841 ccagtgagat tagtcaggag gcacttcaag acatcatgcg tgctgttgag ggagaacctt
    27901 atgcagatgt attgtccatg atgcttcttc gctctatgca gcaggctcgt tattcggagc
    27961 acaatcacgg ccactatgga ctagctgctg actattatac tcactttacc agtccaattc
    28021 gtcgttatcc agaccttctt gttcaccgta tgattcggga ttacggccgt tctaaggaaa
    28081 tagcagagca ttttgaacaa gtgattccag agattgcgac ccagtcttcc aaccgtgaac
    28141 gtcgtgccat agaagctgag cgtgaagttg aagccatgaa aaaggctgag tatatggaag
    28201 aatacgtggg tgaagagtat gatacagttg tatcaagtat tgtcaaattc ggtctctttg
    28261 tcgaattgcc aaacacagtt gaaggcttga ttcacatcac taatctacct gaattttatc
    28321 atttcaatga gcgtgatttg actcttcgtg gagaaaaatc aggtatcact ttccgagtgg
    28381 gtcagcagat ccgtatccgt gttgaaagag cggataaaat gactggagag attgattttt
    28441 cattcgtacc tagtgagttt gatgtgattg aaaaaggctt gaaacagtct agtcgtagtg
    28501 gcagagggcg tggttcaaat cgtcgttcgg ataagaagga agacaagaga aaattaggac
    28561 gctcaaatga taagcgtaag cattcacaaa aagacaagaa gaaaaaagga aagaaacctt
    28621 tttacaagga agtagctaag aaaggagcca agcatggcaa agggcgaggg aaaggtcgtc
    28681 gcacaaaata aaaaggcacg ccacgactat acaatcgtag atacgctaga ggcagggatg
    28741 gtcctgactg gaactgaaat caagagtgta cgagctgctc gaattaatct caaggatggc
    28801 tttgctcaag tgaaaaatgg agaagtttgg ctgagcaatg ttcatatcgc gccttacgaa
    28861 gagggcaata tctggaacca ggaaccagaa cgtcgtcgta aactcctgct ccataaaaag
    28921 caaattcaaa aattggaaca agagaccaaa gggacaggaa tgaccttagt tccccttaag
    28981 gtctatataa aagatggcta cgctaagctt cttttaggac ttgccaaagg gaagcatgac
    29041 tatgacaaac gggagtctat caaacgtcgt gagcaaaatc gagatatcgc gcgtgtgatg
    29101 aaagctgtta atcagcgata aaaagaggaa ttgaaaatgg aaaaattagt tgcctataaa
    29161 cgcatgcctt tgtggaataa acaaacaatg cctgaagctg ttcagcaaaa gcacaataca
    29221 aaagttggga cttgggggaa aattactgtc ttgaagggag ctctcaagtt tattgaattg
    29281 acagaagaag gggaagttct agctgaacac ctctttgaag caggggcaga caatccaatg
    29341 gcccaacctc aagcctggca ccgagtggaa gctgccacag atgatgtgga atggtacttg
    29401 gaattttatt gtaaacctga ggattatttt gctaaaaaat acaataccaa tcctgttcat
    29461 tcagaggtcc tagaggccat gcagacagtg aaacaaggga aagctttgga tttaggttgt
    29521 ggtcaggggc gtaattctct ttttctagcc cagcaagatt ttgatgtgac agctgtagat
    29581 caaaatggac tagctcttga aatcttgcaa agcattgtgg agcaggaaga tttggacatg
    29641 cctgttggcc tttacgatat caattcagct agcattgaac aagaatatga ttttatcgtt
    29701 tcaacagttg ttctcatgtt tctacaagcg gaccgcattc cagctattat tcaaaatatg
    29761 caggagaaaa ccagtgttgg tggttacaac cttatcgttt gtgccatgga cacggaggat
    29821 tatccttgct cggttaactt cccattcacc tttaaagaag gagaactggc agactattac
    29881 aaggattggg aattggttaa gtacaatgaa aatccaggcc atttgcatcg tcgcgatgag
    29941 aatggcaatc gtattcaact acgctttgcg accttactag ctaagaaaat caagtaaaca
    30001 cacatgaaga ttaggaattt tcctgatctt ttttcttttt tacgaatgat atagaaaagg
    30061 agggaattca tgtttgttgc gagagatgct aggggagaat tggtaaatgt gttagaggat
    30121 aaacttgaga agcaagcata cacctgccca gcttgtggag gccagctcca tttgcgtcaa
    30181 ggaccaagtg tacggacgca ttttgcccat aaatccttaa aagactgtga ttttttcttt
    30241 gaaaatgaaa gtccagaaca cctggccaat aaggaatccc tctatcactg gttgaaaaaa
    30301 gagacaaagg ttcaattaga gtacccgctt tcagaactta aacagattgc ggatgtattt
    30361 gtaaatggca atctagctct agaagttcag tgtagtccct tgcctcagaa agtccttaaa
    30421 gagcgaagtg agggctatcg tagtcagggt taccaagtac tgtggttgct gggtcaaaaa
    30481 ctgtggctca aggagcgttt gactcgtcta cagcaaggtt ttctttattt cagtcaaaac
    30541 atgggctttt atgtttggga attagacaag gaaaaacaag ttttaagact caaatacctg
    30601 atttaccagg atctccgcgg taaactccat tatcaaatca aggaattttc ctatggtcaa
    30661 ggtagtttat tggaaatatt gcgtcttccc tataagagac aaaaaatatc tcattttaca
    30721 gtttctgagg acaaggacat ctgtcgctat atccggcaac aactttatta tcaaaatctc
    30781 ttttggatga aagaacaagc agaagcctat caaaagggag aaaatatcct gacttatgga
    30841 ctgaaagaat ggtatccaca aattcgacca atagtgggca aatttttcca gattgaacaa
    30901 gacttgacta gctattatca gcacttttat acctattacc aaaaaaatcc tcaaaatgat
    30961 tggcaaaagc tttatccacc agccttttat cagcaatatt tcttgaaaaa tatggtagaa
    31021 tagaaaggat ggaggaatct aatggtatta caaagaaatg aaataaatga aaaagataca
    31081 tgggatctat caacgatcta cccaactgac caggcttggg aagaagcctt aaaagattta
    31141 acagaacaat tggagacagt agcccagtat gaaggccatc tcttggatag tgcggataac
    31201 ctactagaaa tcactgaatt ttctcttgaa atggaacgcc agatggagaa gctttacgtt
    31261 tatgctcata tgaagaatga ccaggataca cgtgaagcta agtatcaaga gtactatgcc
    31321 aaggccatga cactctacag ccagttagac caagcctttt cattctatga gcctgaattt
    31381 atggagatta gcgaaaagca gtatgctgac tttttagaag ctcaaccaaa gctgcaggtt
    31441 tatcaacact attttgacaa gctcttgcaa ggcaaggatc acgttctttc acaacgtgaa
    31501 gaagaattat tggctggagc tggagaaatc tttggttcag caagtgaaac cttcgctatc
    31561 ttggacaatg cggatattgt gttcccttat gtcctagacg atgatggtaa agaagttcag
    31621 ctatctcatg ggacttacac acgtttgatg gagtctaaaa aacgtgaggt tcgccgtggt
    31681 gcctatcaag ctctttatgc gacttacgaa caattccaac acacctatgc caaaactttg
    31741 caaaccaatg ttaaggtgca aaattaccgt gctaaagttc gtaactacaa gagtgctcgt
    31801 catgcagccc tcgcagcgaa ttttgttcca gaaagtgttt atgacaattt ggtagcagca
    31861 gttcgcaagc atttgccact cttacatcgc tatcttgagc ttcgttcaaa aatcttgggg
    31921 atttcagatc tcaagatgta cgatgtctac acaccgcttt catctgttga atacagtttt
    31981 acctaccaag aagccttgaa aaaagcagaa gatgctttgg tagtcttggg tgaggattac
    32041 ttgagccgtg ttaaacgtgc cttcagcgag cgttggattg atgtttacga aaatcaaggc
    32101 aagcgttcag gtgcctactc tggtggttct tacgatacca atgcctttat gcttctcaac
    32161 tggcaggaca atctggacaa tctctttact cttgttcatg aaacaggtca cagtatgcat
    32221 tcaagctata ctcgtgaaac tcagccttat gtttacggag attactctat ctttttggct
    32281 gagattgcct caactaccaa tgaaaatatc ttgacggaga aattattgga agaagtggaa
    32341 gacgacgcaa cacgctttgc tattctcaat aacttcctag atggtttccg tggaacagtt
    32401 ttccgccaaa ctcaatttgc tgagtttgaa cacgccattc accaagcaga tcaaaatggg
    32461 gaggtcttga caagcgattt cctaaataaa ctctacgcag acttgaacca agagtattat
    32521 ggtttgagta aggaagacaa tcctgaaatc caatacgagt gggctcgcat tccacacttc
    32581 tactataact actatgtata tcaatattca actggctttg cggccgcctc agccttggct
    32641 gaaaaaattg tccatggtag tcaagaagac tgtgaccgct atatcgacta cctcaaggca
    32701 ggtaagtcgg actatccact taatgtcatg agaaaagctg gtgttgatat ggagaaggaa
    32761 gactacctca acgatgcctt tgcagtcttt gaacgccgtt taaatgagtt tgaagccctt
    32821 gttgaaaaat taggattggc ataaaatggt tgaatcgtat agtaagaatg ctaaccataa
    32881 catgcgtcgt cctgtcgtca aagaagaaat tgtagacttg atgcgtcagc gtcaaaagca
    32941 ggtcacaggt ttcttgaaag aattggaaga ctttgcccgc aaggaaaata ttcctattat
    33001 tccccatgaa acggttgctt atttccgttt tcttatggaa accatgcagc ctaaaaatat
    33061 tctggaaatt gggacggcta tcggtttttc agctctcttg atggctgaac atgcgccaaa
    33121 tgctaagatt acaactattg atcgtaatcc agaaatgatt ggttttgcca aggaaaattt
    33181 tgcccagttt gacagtcgca agcaaatcac tctcctagag ggagatgcgg tggatgtctt
    33241 atctacactg acagagtctt atgatttcgt ctttatggat tctgccaagt ctaaatacat
    33301 cgtctttctg ccagaaatcc tcaaacattt ggaagttggt ggtgtggttg tcttggatga
    33361 tatttttcaa ggcggtgatg ttgccaagga cattatggaa gtccgtcgag gtcagcgaac
    33421 catctatcga ggccttcaaa gactatttga cgcaacctta gacaattcag aactcaccgc
    33481 aacattagtg cctttaggag atggtattct catgcttcgt aaaaatgtag cagatgttca
    33541 attgcctgac agcgaatgat tttcagaaaa atttaagaaa aaatagtaaa atagatagag
    33601 taacacttat ctcaaaggag tagacatgaa gaaaaaatta ttggcaggtg ccatcacact
    33661 attatcagta gcaactttag cagcttgttc gaaagggtca gaaggagcag accttatcag
    33721 catgaaaggg gatgtcatca cagaacatca attttatgag caagtgaaaa acaacccttc
    33781 agcccaacaa gtcttgttaa atatgaccat ccaaaaagtt tttgaaaaac aatatggctc
    33841 agagcttgat gataaagagg ttgatgatac tattgccgaa gaaaaaaaac aatatggcga
    33901 aaactaccaa cgtgtcttgt cacaagcagg tatgactctt gaaacacgta aagctcaaat
    33961 tcgtacaagt aaattagttg agttggcagt taagaaggta gcagaagctg aattgacaga
    34021 tgaagcctat aagaaagcct ttgatgagta cactccagat gtaacggctc aaatcatccg
    34081 tcttaataat gaagataagg ccaaagaagt tctcgaaaaa gccaaggcag aaggtgctga
    34141 ttttgctcaa ttagccaaag ataattcaac tgatgaaaaa acaaaagaaa atggtggaga
    34201 aattaccttt gattctgctt caacagaagt acctgagcaa gtcaaaaaag ccgctttcgc
    34261 tttagatgtg gatggtgttt ctgatgtgat tacagcaact ggcacacaag cctacagtag
    34321 ccaatattac attgtaaaac tcactaagaa aacagaaaaa tcatctaata ttgatgacta
    34381 caaagaaaaa ttaaaaactg ttatcttgac tcaaaaacaa aatgattcaa catttgttca
    34441 aagcattatc ggaaaagaat tgcaagcagc caatatcaag gttaaggacc aagccttcca
    34501 aaatatcttt acccaatata tcggtggtgg agattcaagc tcaagcagta gtacatcaaa
    34561 cgaatagtcc aaatcaatga gtcagggaaa aaactcgact tcaggaaaaa atgaagcaaa
    34621 cattcccaca ataaaacgca tagtacaagg tttgtactgc cccccaaaaa agttagacaa
    34681 ttaatttatc cgaaggattt agttctgtat tgcacagagc taagtccttt tagttttatc
    34741 ttaattctct tattgttgta ataatcaata tagtctataa tggcttgttc caattgatta
    34801 agtgatttaa atgtttgctc atagccataa aacatttcgg attttaaaat gccaaagaaa
    34861 gattccatcc taccgttgtc ttggctgttt cctttgcgtg acatagatgc ttgaattccc
    34921 ttactctcta ggaagcgatg ataagaatcg tgttggtatt gccagccttg gtcactatgg
    34981 agaatcgtat tctcgtagtg cttctctttg aatgcctgtt ccaacattaa cgatcaatca
    35041 atttaatcat gtacctaata ttagaattgt ttatctcaaa tttatttgaa agtttctcta
    35101 agctatatcc ttgttttcta agttcataga tctgaacttt atcatcataa gttaatttca
    35161 taataaaaac accccaaaag ttagattttt tctgtctaac ttttgggggg cagttcattt
    35221 aacacctgat aatatgcgtt ttttgatttt aaagactttt tgaccagtct cattttttta
    35281 acttgatact cagtgcaaag caaagattaa actaggaagc tagctgtagg ctgctcaaag
    35341 aacagctttg aggttgtaga taaaacttgt gaggtcacca acatatataa tgtgaagctg
    35401 acgtggtttg aatagatttt agaagagtat gagtctggaa gttttaatgg ataatgcaag
    35461 attccataga atgggtaagc tagagttctt atgtgaagag tttgggcata aacttttacc
    35521 ttttcctccc tactcatctt agtatagaaa agtgaatctg aaatagtaca taactgcttc
    35581 taaaacattc ttataaattg atttaaattc tcaaatcata ttattcagtt cttatttcat
    35641 tttgttctac aatcctgttg agaagacacg tgttcatatc aaaaaggtat tggcaagttg
    35701 caataccttt ttacgaggct ctgttgtctt atttttgttt caactgacta tatctcctat
    35761 ggttctagtt cagaaggcta ggctataatt atgattgata agaagtatca ttccaagtat
    35821 tgggagtgaa tgtttcaaaa tcatgggttt ctataatggt caggctggca tttgctagac
    35881 cgccatcttt acgaagaagt ggttctttat agcctaggag agtacgaaga ctggcagtaa
    35941 gattggcgcc gtgtccgaca attagaatac gttcagctgg actatctttt aatgatttga
    36001 taaattggat ggtccgttga gttgtactat agagggattc ggatccgaac attcgagtgt
    36061 caaattgagc aagatttgaa cgaaaagcct ggatttgttg cgggtaaata gcttccaagg
    36121 ttgcaatttt caaaccttct aacttcccaa gttgccattc acggagatta ggaacgattt
    36181 ctaaagaaca gggggtatag agttgacttt ggataatctc agcagatttg accgctcgag
    36241 gtaaatcact tgaataaatc tgatcaaaag gaatttcctt gagatactga ccaagtcgtt
    36301 ttagggtttc aatggattca ggaagaagag gagaatcacc actagcacct tgaaaacgac
    36361 cttcttggtt ccagagggta cgaccgtggc ggacaaagta gagtttcatt acttgatgtc
    36421 ctccaaaata tctacaaagt ctgcctttac aaagctagcc aagtcttgtg gcgcgacgat
    36481 aatgctgtgt ccgacttcgc ctgcagagac aatcatttga tccaaatcta gagcaatttt
    36541 atcgataaaa atgggataat tgtgtttctg acgaattccg acaggattat tggctccatg
    36601 aatgtaacca gttgtttttt ctaagtcctt ttgtggaatc atgctcactt ttttattgcc
    36661 agaaatttta gctagtttct tttcagacaa gtgctgagtg atagggacaa ttccgataat
    36721 cggtccggtc ttgtctccca aaagcgccaa ggttttgaaa atctgatctc gttcataacc
    36781 ttgaggaagc tctccttcta gggcattgat ttgaatcccc tgatgaggga tagctgcttt
    36841 agataggatt tgttccacca atgttttttt gattttaact ttttttgcca ttatttatat
    36901 ttatcctcca attgactcat ccaaatacca agccagattc ccagcgcaaa gaagaaggcg
    36961 atgatgacat aaccgacaag tgaaagtcct gtgtattgga tactttcagc gtttcctgca
    37021 tttggaatta agatcaaaag ggtacttgat aggacgatac cgatgatgaa atgatagacg
    37081 cgtgagtggt agttgtttaa ggcataatcc atcaattttg aaaaaacgat gagagttgca
    37141 cctgcaccaa ttccaatcgg aaagaaggtt cccaagagat caaaagtttt aaaaccagtc
    37201 aacataggag catagagtcc caaaatcaaa agtaagttcg atgggctgag gccaggaacc
    37261 aagacgccaa gggccaatag tgcgcctgct aggacgaagt taagaaagct ggcgcttaag
    37321 gttccaacga caaaatttaa ggcatagagt cctaatccag aaatgataaa ggttgtccat
    37381 aaccaagcta aatcaatctt gtctcggtca gattctcgag ttgattcttt gaggaggcta
    37441 ggaactgtac cgataatagc tcccgcaaag ctccataata caaagacctg ataattttca
    37501 agcaggtatt caatcgggta ggaaaataag ccgattccca gaagcatacc gatggcaact
    37561 ggaataaagt acaaaacatt ttctttaagg tctttaaagg gatgggccag aaagccaatc
    37621 attcgttcat agattcctaa gattgctgct agaacccctc cggaaattcc cggtaggata
    37681 aatccaagag caattacaat ccctttaata acgcgtgcta accatgagag catatatttc
    37741 ctccaactat ttctatttca aaaattcctt actatattgt atcacaatca gacacaaaaa
    37801 gaaaaagcaa atgataaaca aatgctttta aggttttaaa aaaagttttc gaaaggtttc
    37861 ttctttattt tttaagggag agataacgtt gatatctaaa tcgtggtcaa agccggcaat
    37921 ttttccttta gatgtgtatt ggtgaatatc ataatctaaa tcagttttag gactgctctc
    37981 caaaaatcct gagtctgagc cgtaggacgg aatccaaaca gaggtaaact tgcctgtatc
    38041 aatactgtgt tcttccatga agtagacacc aacgtagatg ccgatgtttt tagcacctag
    38101 tgatgctagt tttgctcgaa agttttcgac accttcgttc atattagaca tggttttgtc
    38161 ttccacgtca agccaatagt aactagggct gtaaggagag gcagcattgt agaaaacttc
    38221 ggcagccttt tccatttctt ggacactttt tccagctaca taagcgtaga cagcaactgg
    38281 gacattccgc ttttgaagtt cagtgatatg actcttatag gccttgtcta ttccattgat
    38341 aaatgaagca tcattttctt ttgttgtttg agcaccactg tgaacacgaa caatagcacc
    38401 tgaaatattt tgtgagaggg catcgtagtt gatttcctca ggacgctgcc agccagagag
    38461 gtcaataatc ggtttgtcta agtgtttcaa agcctgtgct tcaatctgtg ctatattgga
    38521 ttttgtttta aacgattggc tgtcattaag tgggcgattg atgattaaaa tgaacatcat
    38581 aatcccaaaa aaactaaata aaataagtgg atgaatttgt tttctcatat cttataagtc
    38641 taccctaaaa atcaaaaaaa atcaaaaaaa tgggttaagg aagagacttt agagcatttt
    38701 ttcattcaag agtgcggaat gatttgaaat atggtataat aaaagggaat ttctacagaa
    38761 aagagaagat tatgtcaaat tttgccatta ttttagcagc gggtaaaggg actcgcatga
    38821 aatctgattt gccaaaagtt ttgcacaagg ttgcgggtat ttctatgttg gaacatgttt
    38881 tccgtagtgt gggagctatc caacctgaaa agacagtaac agttgtagga cacaaggcag
    38941 aattggttga ggaggtcttg gctggacaga cagaatttgt gactcaatct gaacagttgg
    39001 gaactggtca tgcagttatg atgacagaac ctatcttaga aggtttgtca ggacacacct
    39061 tggtcattgc aggagatact cctttaatca ctggtgaaag cttgaaaaac ttgattgatt
    39121 tccatatcaa tcataaaaat gtggccacta tcttgactgc tgaaacggat aatccttttg
    39181 gctatggacg aattgttcgt aatgacaatg ctgaggttct tcgtattgtt gagcagaagg
    39241 atgctacaga ttttgaaaag caaatcaagg aaatcaacac tggaacatac gtctttgaca
    39301 acgagcgttt gtttgaggct ttgaaaaata tcaataccaa taacgctcaa ggcgaatact
    39361 atattacaga cgtcattggt attttccgtg aaactggtga aaaagttggc gcttatactt
    39421 tgaaagattt tgatgaaagt cttggggtaa atgaccgtgt ggcgcttgcg acagctgagt
    39481 cagttatgcg tcgtcgcatc aatcataaac acatggtcaa cggtgttagc tttgtcaatc
    39541 cagaagcaac ttatatcgat attgatgttg agattgctcc ggaagttcaa atcgaagcca
    39601 atgttacctt gaaagggcaa acgaaaattg gtgctgagac tgttttgaca aacggtactt
    39661 atgtagtgga cagcactatc ggagcaggag cggtcattac caattctatg attgaggaaa
    39721 gtagtgttgc agacggtgtg acagtcggtc cttatgctca cattcgtcca aattcaagtc
    39781 tgggtgccca agttcatatt ggtaactttg ttgaggtgaa aggatcttca atcggtgaga
    39841 ataccaaggc tggtcatttg acttatatcg gaaactgtga agtgggaagc aacgttaatt
    39901 tcggtgctgg aactattaca gtcaactatg acggcaaaaa caaatacaag acagtcattg
    39961 gagacaatgt ctttgttggt tcaaattcaa ccattattgc accagtagaa cttggtgaca
    40021 attccctcgt tggtgctggt tcaactatta ctaaagacgt gccagcagat gctattgcta
    40081 ttggtcgcgg tcgtcagatc aataaagacg aatatgcaac acgtcttcct catcatccta
    40141 agaaccagta ggagcctatc atggagtttg aagaaaaaac gcttagccga aaagaaatct
    40201 atcaaggacc aatatttaaa ctggtccaag atcaggttga attaccagaa ggcaagggaa
    40261 ctgcccgacg ggatttgatt ttccacaatg gggctgtctg tgttttagca gtaacggatg
    40321 aacaaaaact tatcttggtc aagcagtacc gcaaagctat cgaggctgtc tcttacgaaa
    40381 ttccagccgg aaaattggaa gtaggagaaa acacagcccc tgtggcagct gcccttcgtg
    40441 aattagagga agaaacagcc tatacaggga aattagaact cttgtacgat ttttattcag
    40501 ctattggctt ttgtaatgag aagttaaaac tatatttagc aagcgatttg acaaaagtgg
    40561 aaaatccgcg tccgcaggat gaggatgaaa ccttggaagt ccttgaagtg agcttagaag
    40621 aagcgaaaga attaatccaa tcaggtcata tctgtgatgc caagacaatt atggctgttc
    40681 agtattggga gttgcagaaa aaatagagga ggtcagtatg ggtaaatctt tattaacgga
    40741 tgaaatgatt gaaagagcta atagaggcga aaaaatttca ggtcctcctt tgctagatga
    40801 taatgaggaa actaagattt taccaacctc ttcttcccgt tttggttatg ccaatcctaa
    40861 ggatcatggt tttagccagg aaaccttgaa gattcaggtc gaaccatcta ttcataaaag
    40921 ccgtcgtatt gaaaatacca agagaaatgt cttcaattct aagttgaata aaatcttatt
    40981 tgcggtcatc tttctcttga ttttgcttgt tttagcaatg aaacttttgt aatagaaaag
    41041 gaattgaaat gaaaatagga attattgctg ctatgccaga agaactggct tatctggtcc
    41101 agcatttaga taatacccag gagcaagttg ttttggggaa tacctatcat acaggaacca
    41161 ttgcttctca tgaagtcgtt cttgtagaaa gtggaattgg taaggtcatg tctgctatga
    41221 gtgtggcgat tttggctgat catttccagg tggatgccct tattaatacg ggttcagctg
    41281 gggcagtagc agaaggtatc gctgttgggg atgtcgtgat tgctgacaaa ttagcctatc
    41341 atgacgtgga tgtcacagct tttggctatg cttatggaca aatggcgcaa caaccgcttt
    41401 atttcgaatc agacaaaacc tttgttgctc aaatccaaga gagtttatct caattggacc
    41461 aaaactggca tcttggtttg attgctacag gagatagttt tgttgcagga aatgacaaga
    41521 tagaagcgat taagtcccat ttcccagaag ttttagccgt ggagatggag ggggcagcta
    41581 ttgctcaagc agcgcatgcc ctcaatctcc cagtcttagt catcagagct atgagtgaca
    41641 atgccaacca tgaagcaaac atcttttttg atgagtttat tatcgaagct ggacgtcgct
    41701 ctgcccaagt cttgttgacc tttttgaagg ctttagatta agcggaaatt tgacagtttt
    41761 tctagcttat gataagattt aagtaaagaa aagctagaaa acgtttcaga ggatattatg
    41821 agtattgaaa tgaccgtcag tgagattgca gaggtcttag gattatctcg ccaagcaatc
    41881 aataaccgtg tcaaagaatt accagaagaa gacacagata aaaatgacaa aggggtaaca
    41941 gttgttacca gaagtggctt gattaagcta gaagaaatct ataaaaaaac gatttttgaa
    42001 gatgagcctg tcagtgaaga tgtcaaacaa cgtgaactga tggagattct agtggatgag
    42061 aagaatgcag aaattcttcg tctttatgaa caattaaaag ccaaggatcg tcagttatca
    42121 gaaaaagacg agcagatgcg tatcaaagac cgtcagattg ctgagaaaga caaacaatta
    42181 gatcagcaac aacaattgac ccttcaagcc atgaaggatc aagaaaatct taagctagag
    42241 ctggaccaag caaaagaaga agtccaatcc actaagaaag gcttttttgc tcgtttattt
    42301 ggaggataat caaggagctg tttaggttaa atgctaacct gatggcttct tttgtttgtg
    42361 aatagtatag ttgattagaa cagagacagt agaggagagg tacagaatgg aacgattaga
    42421 agattacatt gcttttgact tagaatttaa tcagcacgaa ggacaaacac acttgattca
    42481 agtatcggca gtgcgtttta gagatggtcg ggaaatcgat gcctatgatt cctatgttca
    42541 taccagcgtc cctctaaaaa gctttatcaa tggtttgacc gggattacag ctgagacctt
    42601 aaaagatgct cctccagttg agaaagtctt agaagaattc caagagtttg tgggttcttt
    42661 gctgatgata ggctacaatg ctgctaaaag tgatttacct attttagcag agcatggttt
    42721 agactatagc caacagtaca aggtggattt gtatgatgaa gcttttgaac gtcggagttc
    42781 agatttacat ggtattgcca atctcaaatt gcaaactgtt gcctcatttc ttggatttca
    42841 aggtcagtct cataatagtt tagaggatgc tcggatgacg gcgcgtgttt acgaatcctt
    42901 cttagagtcg gatcaagcta gggaattgtt agagacagaa agtagcttat caaacaatcc
    42961 ttttggtgtc ttggacttgt ctcaattatt tgactaggag tgcctatgaa acctgaaaaa
    43021 cctaaaaaat tgggtcttaa acagatttac ctcaatcttg ataaaatcct ctttctattt
    43081 ttcttgattt ttatgatagt ggagtatttg tgggtaccat tcaattcatg gttggctagc
    43141 ttcttattag atcagacagg ttatcttttt atttcctata acaatgtctt agccatcctt
    43201 actcattctc cattgattag tctagctttt ttagccttga ttgtaattaa ccttctggtt
    43261 gcttattttc aaataggact cctctttatt ggtgctcgtc atctcctcta taatgaaaag
    43321 cgaaggctaa ttgagtatat ccgcaaggtt ttccatgaaa gttttggatt tatgaaaaag
    43381 ctaacttgcg ctaaagcctt gtttatcttt ttctatatag ctatgctttt tccttttata
    43441 cggaaaatgc tgaagattta ttatcttaat aaaatcgtca ttccagagtt tatccaagct
    43501 tatttagaag ataaatattg gatgtggtgg ctgagcatcc tacttctatc tcttatcttt
    43561 ctctatgtct ctgtcagatt gatgtttgcc cttccgaaga ttgtatatga tcagctgact
    43621 gttcgagaag cagtgatgtt tagcttggag aaaaccaaga agagagttac tttttatgct
    43681 tggaatttat tttacatctt gctcaaagca catttattat tttatcttcc tttgattcct
    43741 ttattattgg ctcaaacttt ggtagatgat atcactcaga gagagtctct gattcttggt
    43801 attttgaatt ttgtcgtgat taaaaatctc tattatatgg ctctgactta ttttttggtt
    43861 aaatttgttt catttttgac aggaaaggag ctggatatgc ttcctaggag agaaaaagat
    43921 cacatcgtgc gatggggtgt catgacttgt gccagtcttt tctttgcctt agaaggttat
    43981 atttatctgg aggctcccat ggttcatcta cctcaactta tttctcaccg aggggtttcc
    44041 aatgcaaatg ggattcaaaa tacagtagag tccttggaaa ctacagcaca actcaaacca
    44101 gacttggtgg agacggacgt gcaggaaaca aaagatgggc agtttgtcat gatgcatgat
    44161 gctaacttga aaaatctagc aggtatcaat aaaagtcctc aagacttaaa cttggaggag
    44221 cttaaaggga ttgatatttt tgaaaatggc taccagacta aaatttcaag ctttgaagat
    44281 tatctcagtc gagccaacga acttggtcaa aaattactaa ttgaaattaa aaccagtaaa
    44341 aaagatagtc cagacatgat gaaccgcttt ttagcccgtt atgctgcaaa gctcaagatt
    44401 tatggacatc aaatccagtc tttagactac catgttgtcg aaaaagtaag acagtatgat
    44461 gccgaactgc cagtttattt catcatgccc tacaattctg tctttcctaa aacaagagcg
    44521 acaggatata ctatggagta ctcaaccttg gatgaatatt ttgtaagcaa actatggaca
    44581 acggatcaga aactttatgt ctggactgtc aatgattcag aagctatcag caaatctctt
    44641 cacttaggag tagatggagt gattacagat gatttggaaa aagttcagcg ggaaatagaa
    44701 gtagcccaag aagatccaga atatacggat ttgctcttga aaaaagcatt ggaattcttt
    44761 gaattttagt aataaaaaag aggaacggtt gttcctccca tgataggtaa gtattcttgt
    44821 gaatgcttgc ttttttgtgg ttgcaatccg tttacgagaa ttgtttgtat tgttcttgtt
    44881 tcaatttact ttatagtgct ttgaagttgg aatgctacac tataacttct aaaatatagt
    44941 gaaatgaacc aaaaatagta cacaatgtgg tataatcttc ttatggcata ttcaatagat
    45001 tttcgtaaaa aagttctctc ttattgtgag cgaacaggta gtataacaga agcatcacac
    45061 gttttccaaa tctcacgtaa taccatttat ggctggttaa agctaaaaga gaaaacagga
    45121 gagttaaacc accaagtaaa aggaacaaaa ccaagaaagg ttgatagaga tagacttaaa
    45181 aactatctta ctgacaatcc cgacgcttat ttgactgaaa tagcttctga ctttggctgt
    45241 catccaacta ccatccacta tgcgctcaaa gctatgggct acactcgaaa aaaagaacca
    45301 cacctactat gaacaagacc cagaaaaagt agccttattt cttaaaaatt ttaatagttt
    45361 aaagcaccta gcacctgttt agattgatga aacaggattc gatacttatt tttatcgaga
    45421 atatggtcgt tcattaaaag gtcagttaat aagaggtaaa gtatctggaa gaagatatca
    45481 gaggatttct ttggttgcag gtctaacaaa tggtgagtta atcgctccaa tgacttacga
    45541 agagacgatg acgagcgact tttttgaagc ttggtttcag aagtttctct taccaacatt
    45601 aaccacacca tcggttatta tagtaaaatg aaataagaac aggacaaatc gatcaggaca
    45661 gtaaaatcga attctaacaa tgttttagaa gcctatatgt actattctag tttcaatata
    45721 ccataactgc tcctagtcaa cctgcaagtc ctgtcccagc tccaagctca gctccgactc
    45781 aagatcctag agtggtagca agcgcttcgg ctagctcaac gagcacacaa gctcaagagc
    45841 aagttgacaa gtctgaactt cgtgccttga gtcaagagtt agaccaacgc ttgaaagctt
    45901 tggcaacagt atctgatcca aaaatcgatg caacccaggc tgtcctccta gatgctcaaa
    45961 aagctccgga agatagtgcc ttgacagagt aaggtctgca tactgcagta gagtctgtga
    46021 aggctgcctt ggactctctc aaagatgtga aagcaaatgc tagcgacagc aagcctgcac
    46081 aggacaagaa ggatgcaaaa caaggaacgg aagatagtaa ggattcagat aagatgactg
    46141 aaacaaactc agttccggca ggagtgattg tggtcagtct acttgccctc ctaggcgtga
    46201 ttgccttctg gctgattcgc cgtaagaaag agtcagaaat ccagcaatta agcacggaat
    46261 tgatcaaggt tctaggacag ctagatgcag aaaaagcgga taaaaaagtc cttgccaaag
    46321 cccaaaacct tctccaagaa acccttgatt tcgtgaaaga agaaaatggc tcagcagaga
    46381 cagaaactaa actagtagag gagcttaaag caatccttga caaactcaag taaaacatga
    46441 tttatgaaat gtcagaccta aaagacattt tcgttcacaa aagcagtgac gagtaatcct
    46501 tgtcgttgct ttttggctat tttgggggat aagatccgcc ttgactagca aaagaaaaga
    46561 ttggtccaat agtgcccgat acgattttgc gtagcagaaa gcttacaaat aaaattgaat
    46621 atagtaaaat aggattagaa ttattaagaa agttggttct ttattggaaa cgatagtatt
    46681 tttaatctct gtttttctag caggtgtttt atcctttttt tctccttgta tttttcctct
    46741 tctgccagtc tatgctggga ttttattgga tgatcaagaa agtgcaaaaa gcttttcttt
    46801 gtttgggaga aaggttctct ggtcaggctt gattcgaaca ctttgcttta tcgctggtat
    46861 ctctctcatt ttctttattc taggctttgg tgctggttac tttggtcata ttctctatgc
    46921 aaattggttt cgatatgtca tgggagctat tattatcatt ttgggtcttc accagatgga
    46981 aatttttcat ttgaagaaat tagaagttca aaaaagtttt acctttaaaa aatcagattc
    47041 taatcgttat tggtcagctt ttttacttgg tattaccttt agctttggtt ggacaccttg
    47101 tattggtcca gttttaagtt ctgttttagc acttgcggct tctggaggca atggtgcttg
    47161 gcaaggagcg atttatactc tcatttacac tctgggcatg gcccttcctt tcttggtctt
    47221 gacactagct tcaggtctag tcatgccata ttttagtaaa atcaagcgtc atatgatgct
    47281 actaaagaaa attggtggtt tcctcattgt tttaatggga attttgttac tattaggaca
    47341 agtaaatgtt ctagctggaa tttttgaata aaggaggaca aatgaaaaaa gtaatgtttg
    47401 ctggcttaag tctcttgtca ttagttgtat tgatggcctg tggtgaggaa gaaactaaaa
    47461 agactcaagc agcacaacag ccaaaacaac aaacgactgt acaacaaatt tctgttggaa
    47521 aagatgctcc agactttaca ttgcaatcca tggatggcaa agaagttaag ttatctgatt
    47581 ttaagggtaa aaaggtttac ttgaagtttt gggcttcatg gtgtggtcca tgcaagaaaa
    47641 gtatgccaga gttgatggaa ctagcggcga aaccagatcg tgatttcgaa attcttactg
    47701 tcattgcacc aggaattcaa ggtgaaaaaa ctgttgagca attcccacaa tggttccagg
    47761 aacaaggata taaggatatc ccagttcttt atgataccaa agcaaccacc ttccaagctt
    47821 atcaaattcg aagcattcct acagaatatt taattgatag ccaaggaaag attggaaaga
    47881 ttcaatttgg tgctatcagt aatgcggatg cagaagcagc atttaaagaa atgaactaga
    47941 atcgataaaa tctgattaca agatgtagtc agattttggt aacttctaaa actaacttcc
    48001 agtttcccac aacttagctt ttagtatgag aaaaacgcgt gacatcagtg gaaatccgtc
    48061 ttagactaac ttccactggt tttctttttc ttgatatata agggttcaaa agcgaaaaga
    48121 ctcagaaaaa gtgcacgaca aatagcccta aaacagagtc gtcttagagt aacttccagt
    48181 tttgggagag agatggaagt tactttgaga agttacggta gtcagatttt tttagaaaaa
    48241 cattttataa atattcacat atctcctata tttatggtaa aatagaatta tcagtttatt
    48301 ttggagtcaa agatgaatat atttagaaca aagaatgtta gtttagataa aacagagatg
    48361 cataggcatt tgaagttatg ggatttgatt ttgctgggta tcggagccat ggtagggaca
    48421 ggcgtcttta caatcacagg tactgcagct gcaacacttg ctggcccagc cctagtgatt
    48481 tcaatcgtta tttctgcctt gtgtgtggga ttatcagccc tcttttttgc agaatttgcc
    48541 tcgcgagtac ccgctacagg aggtgcctat agttacctct atgctatctt aggagaattc
    48601 cctgcctggt tggctggttg gttaaccatg atggagttca tgacagccat atcaggcgta
    48661 gcttcgggtt gggcagctta ttttaaaggc ttgctctctc aatatgggat agcccttcct
    48721 caggctttaa atggtacctt taatcctcaa gcagggacat ttgttgattt attgcctatt
    48781 cttgtcttgg tcttggtgac ttcgcttgtt ttactaaatg ccaaggcagc cttacgcttt
    48841 aattcgattc tagttatttt gaaattttcc gctttagctc tctttgtttt agtaggaatt
    48901 tggaatatta agtttgataa ttggagcaat tttgctcctt acggctttgg acaaatctat
    48961 ggagctagta ctggtattat ggctggtgcg tccttgatgt tcttcggttt tttgggattt
    49021 gaatccatct ctatggctgt agatgaagtc aagactcctc aaaaaaatat tcctagaggg
    49081 attgtcttat cgctttctat cgtaaccatt ctctatgcct tggtgaccct tgttttgact
    49141 ggtgtggttc actatagtca tttaaatgtc gatgatgccg ttgcctttgc ccttcgtagt
    49201 gttgggatta gttgggcagc caactatgtg tcattagtgg ctatcttgac cttgattaca
    49261 gtttgtattt cgatgaccta tgccctatcg cgtatgattt acagtttagc acgtgacggc
    49321 ttagtgcctg ctgcctttaa ggaactgacg aagactagca aggtaccaaa gaatgctact
    49381 atcttgacag gtctagcttc agcagtagca gcaggaatgt tcccactagc cagtattgca
    49441 gcctttttaa atatttgtac cttagcctac ttgatcatgc tggcttatgg tctgattcgc
    49501 ttacggaaag aaaaaggaat gcccaaagct ggggaattta aaacaccatt ggtacctctg
    49561 ttaccaattt tatcgatcat catttgttta tccttcatgt tacagtataa tatgaatacc
    49621 tggattgctt tcctagttgc gttgttagta ggaagtatta tttacttcac ttatggctat
    49681 aagcattcta ccatagaaga ataaagtgaa agtctattga gttgttgaca ctcagcagat
    49741 ttttatatgt gaaagaaatt tcaaattttt tctaaaaata tcttgacaga ttaaattttc
    49801 aggagtagaa tatttactag ttaattaaat ggttaaggag ttgttcatga agaaacaaaa
    49861 tttattttta gtcctgttaa gtgtctttct tttgtgcttg ggggcttgtg gtcaaaagga
    49921 aagtcagaca ggaaagggga tgaaaattgt gaccagtttt tatcctatct acgctatggt
    49981 taaggaagta tctggtgact tgaatgatgt tcggatgatt cagtcaagta gtggtattca
    50041 ctcctttgaa ccttcggcaa atgatatcgc agccatctat gatgcagatg tctttgttta
    50101 ccattctcat acactcgaat cttgggcagg aagtctggat ccaaatctaa aaaaatccaa
    50161 agtgaaggtc ttagaggctt ctgagggaat gaccttggaa cgtgtccctg gactagagga
    50221 tgtggaagca ggggatggag ttgatgaaaa aacgctctat gaccctcaca catggctaga
    50281 tcctgaaaaa gctggagaag aagcccaaat tatcgctgat aaactttcag aggtggatag
    50341 tgagcataaa gagacttatc aaaaaaatgc gcaagccttt atcaaaaaag ctcaggaatt
    50401 gactaagaaa ttccaaccaa aatttgaaaa agcgactcag aaaacatttg taacacaaca
    50461 tacagccttt tcttatctag cgaagagatt tgggcttaat caacttggta ttgcaggtat
    50521 ctctcctgaa caagaaccaa gtccacgaca actaacagaa attcaggaat ttgttaagac
    50581 ctataaggtt aaaacgattt ttacagaaag taacgcttct tcaaaagtag ctgaaactct
    50641 tgtcaaatca acaggtgtgg gtcttaaaac tctgaatcct ttagagtcag acccacaaaa
    50701 tgacaagacc tatttagaaa atcttgaaga aaatatgagt attctagcag aagaattaaa
    50761 gtgaggaaag aatgaaaatc aataaaaaat atctagcagg ttcagtggca gtccttgccc
    50821 taagtgtttg ttcctatgaa cttggtcgtc accaagctgg tcaggttaag aaagagtcta
    50881 atcgagtttc ttatatagat ggtgatcagg ctggtcaaaa ggcagaaaat ttgacaccag
    50941 atgaagtcag taagagagag gggatcaacg ccgaacaaat tgttatcaag attacggatc
    51001 aaggttatgt gacctctcat ggagaccatt atcattacta taatggcaag gttccttatg
    51061 atgccatcat cagtgaagaa ct
//
LOCUS       DAVKJJ010000008        49512 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000008 DAVKJJ010000000
VERSION     DAVKJJ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 49512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49512
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.008"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            120..911
                     /locus_tag="VW144_000454"
     CDS             120..911
                     /locus_tag="VW144_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEU9599946.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLLDHLGDPSPEIRDDLVFTSF
                     ARGIQEELFTQEQFHFIAEGVSSDVGLDKEIDKVGLPTLERSFRALVYANLLYADANQ
                     QSVFYQGLQLEIRNVFSNQGLHYLSKEKDTTGFSSQYGWIHSFAHGADLLTEVVCHPD
                     FPINRIHEVFDILGQLFKRMSIRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVD
                     FPIEEREDFYKFSNFRSCLVEVYVQLDQRNSLQDDLKEAIQSFQY"
     gene            1022..1690
                     /locus_tag="VW144_000455"
     CDS             1022..1690
                     /locus_tag="VW144_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEU9599947.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGFDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLGN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(1900..2433)
                     /gene="queF"
                     /locus_tag="VW144_000456"
     CDS             complement(1900..2433)
                     /gene="queF"
                     /locus_tag="VW144_000456"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEU9599948.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWAMQHYETVEAVTFAYGQRHHLE
                     IQIAREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKPLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            complement(2739..3053)
                     /gene="trxA"
                     /locus_tag="VW144_000457"
     CDS             complement(2739..3053)
                     /gene="trxA"
                     /locus_tag="VW144_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9599949.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            complement(3070..3291)
                     /locus_tag="VW144_000458"
     CDS             complement(3070..3291)
                     /locus_tag="VW144_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9599950.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            complement(3391..3771)
                     /locus_tag="VW144_000459"
     CDS             complement(3391..3771)
                     /locus_tag="VW144_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9599951.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            complement(3915..4868)
                     /locus_tag="VW144_000460"
     CDS             complement(3915..4868)
                     /locus_tag="VW144_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9599952.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEKIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYGQ"
     gene            complement(4936..6154)
                     /gene="sstT"
                     /locus_tag="VW144_000461"
                     /pseudo
     CDS             complement(4936..6154)
                     /gene="sstT"
                     /locus_tag="VW144_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            6293..6853
                     /locus_tag="VW144_000462"
     CDS             6293..6853
                     /locus_tag="VW144_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEU9599953.1"
                     /translation="MQKFIQAYIEKLDVTSIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            6910..7818
                     /locus_tag="VW144_000463"
     CDS             6910..7818
                     /locus_tag="VW144_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9599954.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLENHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            7770..8360
                     /locus_tag="VW144_000464"
     CDS             7770..8360
                     /locus_tag="VW144_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEU9599955.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            8363..9469
                     /gene="yqeH"
                     /locus_tag="VW144_000465"
     CDS             8363..9469
                     /gene="yqeH"
                     /locus_tag="VW144_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEU9599956.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAYEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            9684..9995
                     /gene="yhbY"
                     /locus_tag="VW144_000466"
     CDS             9684..9995
                     /gene="yhbY"
                     /locus_tag="VW144_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEU9599957.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            10047..10676
                     /locus_tag="VW144_000467"
     CDS             10047..10676
                     /locus_tag="VW144_000467"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU9599958.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRDFIAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            10676..11269
                     /gene="yqeK"
                     /locus_tag="VW144_000468"
     CDS             10676..11269
                     /gene="yqeK"
                     /locus_tag="VW144_000468"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEU9599959.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLDLIDRYQLDFDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEIHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            11270..11770
                     /locus_tag="VW144_000469"
     CDS             11270..11770
                     /locus_tag="VW144_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9599960.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            11783..12136
                     /gene="rsfS"
                     /locus_tag="VW144_000470"
     CDS             11783..12136
                     /gene="rsfS"
                     /locus_tag="VW144_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU9599961.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            12239..12985
                     /locus_tag="VW144_000471"
     CDS             12239..12985
                     /locus_tag="VW144_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9599962.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEAPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            12995..14092
                     /locus_tag="VW144_000472"
     CDS             12995..14092
                     /locus_tag="VW144_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEU9599963.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQSMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHTIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVEAVATKRY
                     TKARVRRLLTYILVQARENVLPEAIHVLGFTEKGRQHLKSLKGQVNLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETS"
     gene            14330..14584
                     /locus_tag="VW144_000473"
     CDS             14330..14584
                     /locus_tag="VW144_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEU9599964.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSIQVHVIEE"
     gene            14588..14842
                     /locus_tag="VW144_000474"
     CDS             14588..14842
                     /locus_tag="VW144_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEU9599965.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            15033..16646
                     /locus_tag="VW144_000475"
     CDS             15033..16646
                     /locus_tag="VW144_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU9599966.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            16776..17402
                     /gene="gmk"
                     /locus_tag="VW144_000476"
     CDS             16776..17402
                     /gene="gmk"
                     /locus_tag="VW144_000476"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU9599967.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            17427..17741
                     /locus_tag="VW144_000477"
     CDS             17427..17741
                     /locus_tag="VW144_000477"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU9599968.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            17807..20203
                     /locus_tag="VW144_000478"
     CDS             17807..20203
                     /locus_tag="VW144_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU9599969.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFDSEDSLAFSSLDLGKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVRRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQMVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSHSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQILTQVAGRAGRAEKAGQVLIQSYNPQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            20216..21151
                     /locus_tag="VW144_000479"
     CDS             20216..21151
                     /locus_tag="VW144_000479"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU9599970.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKSEEEKLNWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            21144..22457
                     /gene="rsmB"
                     /locus_tag="VW144_000480"
     CDS             21144..22457
                     /gene="rsmB"
                     /locus_tag="VW144_000480"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEU9599971.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFTNGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVADRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            22472..23212
                     /locus_tag="VW144_000481"
     CDS             22472..23212
                     /locus_tag="VW144_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU9599972.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDEE"
     gene            23209..25188
                     /gene="pknB"
                     /locus_tag="VW144_000482"
     CDS             23209..25188
                     /gene="pknB"
                     /locus_tag="VW144_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEU9599973.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            25303..26061
                     /locus_tag="VW144_000483"
     CDS             25303..26061
                     /locus_tag="VW144_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9599974.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            26063..26512
                     /locus_tag="VW144_000484"
     CDS             26063..26512
                     /locus_tag="VW144_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9599975.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            26574..27199
                     /locus_tag="VW144_000485"
                     /pseudo
     CDS             26574..27199
                     /locus_tag="VW144_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            27267..27515
                     /locus_tag="VW144_000486"
     CDS             27267..27515
                     /locus_tag="VW144_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9599976.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
     gene            27646..28818
                     /locus_tag="VW144_000487"
     CDS             27646..28818
                     /locus_tag="VW144_000487"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEU9599977.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLNKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            28818..30092
                     /locus_tag="VW144_000488"
     CDS             28818..30092
                     /locus_tag="VW144_000488"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEU9599978.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(30127..31092)
                     /locus_tag="VW144_000489"
     CDS             complement(30127..31092)
                     /locus_tag="VW144_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9599979.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(31073..32527)
                     /locus_tag="VW144_000490"
     CDS             complement(31073..32527)
                     /locus_tag="VW144_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9599980.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLIHFRETGVKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQNLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDCFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANIQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            32782..32908
                     /locus_tag="VW144_000491"
                     /pseudo
     CDS             32782..32908
                     /locus_tag="VW144_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915902.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator T protein"
     gene            32903..34840
                     /locus_tag="VW144_000492"
     CDS             32903..34840
                     /locus_tag="VW144_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEU9599981.1"
                     /translation="MDYSKVAAEVIEAVGKDNLVAAAHCATRLRLVLKDEAKVNQAAL
                     DNNADVKGTFSTNGQYQIIIGPGDVNFVYAEIIKKTGLKEVSTDDLKEIANKDKKFNP
                     LMDLIKLLSDIFVPIIPALVAGGLLMALRNFLTSPDLFGPQSIEDMYPAIKGFSAMIQ
                     LMSAAPFMFLPVLVGISAAKRFGANQFLGAAIGMIMTTPDLGGKEAFWDILGFHVTQT
                     NYAYQVIPVLVAVWLLANLEKFFHKKLPSAVDFTFTPLLSVMITGFLTFTVIGPVMLV
                     VSDAITNAIVWLYNTTGAFGMGLFGGTYSLIVMTGLHQSFPAIETQLLSAYNNNGTGF
                     GDYIFVVASMANVAQGAATLAVYFLTKNAKTKGLSSSAAVSAFLGITEPALFGVNLKY
                     KFPFFCALAGSAIGAFVAGLIHVIAVSLGAAGFIGFLSIKAGSIPMYIIAEIMSFVAA
                     FAFTYFYGKTKAASVFADEAATATAETVTEPTVEAPVVEETDTLQNETLVTPIVGDVV
                     ALADVNDPVFSSGAMGQGIAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLI
                     HVGIDTVSMNGDGFEAKVAQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYA
                     SVAPVATGSVAKGNAVIEVKI"
     gene            34989..35876
                     /locus_tag="VW144_000493"
     CDS             34989..35876
                     /locus_tag="VW144_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9599982.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(36196..36813)
                     /locus_tag="VW144_000494"
     CDS             complement(36196..36813)
                     /locus_tag="VW144_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEU9599983.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(37228..37416)
                     /locus_tag="VW144_000495"
                     /pseudo
     CDS             complement(37228..37416)
                     /locus_tag="VW144_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(37926..38006)
                     /locus_tag="VW144_000496"
                     /pseudo
     CDS             complement(37926..38006)
                     /locus_tag="VW144_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            38123..39016
                     /locus_tag="VW144_000497"
     CDS             38123..39016
                     /locus_tag="VW144_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9599984.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            39013..40212
                     /locus_tag="VW144_000498"
     CDS             39013..40212
                     /locus_tag="VW144_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9599985.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVEVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(40565..41332)
                     /locus_tag="VW144_000499"
     CDS             complement(40565..41332)
                     /locus_tag="VW144_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9599986.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(41349..42044)
                     /locus_tag="VW144_000500"
     CDS             complement(41349..42044)
                     /locus_tag="VW144_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9599987.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            complement(42049..42414)
                     /locus_tag="VW144_000501"
     CDS             complement(42049..42414)
                     /locus_tag="VW144_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9599988.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            42554..43027
                     /gene="nrdR"
                     /locus_tag="VW144_000502"
     CDS             42554..43027
                     /gene="nrdR"
                     /locus_tag="VW144_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEU9599989.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            43028..44197
                     /locus_tag="VW144_000503"
     CDS             43028..44197
                     /locus_tag="VW144_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEU9599990.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLGHPVYRRLLEKKIGDVAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKEATIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRDR
                     GQKAKTQKQNQTAPEKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            44198..45094
                     /gene="dnaI"
                     /locus_tag="VW144_000504"
     CDS             44198..45094
                     /gene="dnaI"
                     /locus_tag="VW144_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEU9599991.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            45091..45804
                     /locus_tag="VW144_000505"
     CDS             45091..45804
                     /locus_tag="VW144_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEU9599992.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            45818..47128
                     /gene="der"
                     /locus_tag="VW144_000506"
     CDS             45818..47128
                     /gene="der"
                     /locus_tag="VW144_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU9599993.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            47219..47554
                     /locus_tag="VW144_000507"
     CDS             47219..47554
                     /locus_tag="VW144_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9599994.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            47694..48029
                     /locus_tag="VW144_000508"
     CDS             47694..48029
                     /locus_tag="VW144_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9599995.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     SLFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGVRH"
     gene            48214..49005
                     /locus_tag="VW144_000509"
     CDS             48214..49005
                     /locus_tag="VW144_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9599996.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            48998..>49512
                     /locus_tag="VW144_000510"
     CDS             48998..>49512
                     /locus_tag="VW144_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9599997.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLLVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFL"
BASE COUNT        14668 a         9489 c        11160 g        14195 t
ORIGIN      
        1 gaaatatgct gtttctctga ctataaatgt ctttttcttt ttaacttaat tttacaaatt
       61 ggtaaggtca tttaagactg cttttttgaa cagttgctag aaatgtagag gtattgacca
      121 tgtatcaaga tttactaaga aaaatagcag aagaaaaacc aaattataac caggaggaaa
      181 tccagtggtt gcttgatcat ttgggagatc cttctccaga aattcgcgat gaccttgttt
      241 ttacaagctt tgctagaggg attcaggaag agctatttac acaggagcaa tttcatttca
      301 ttgctgaagg ggtttcatct gatgtaggac tagataaaga gattgataag gtaggtctgc
      361 caacacttga acgttctttt agggcacttg tttatgcaaa tctcttgtat gcagatgcca
      421 accagcaatc ggttttttat cagggattac aattagaaat tcgtaatgtc ttttcaaatc
      481 aaggcttgca ctatctttca aaagaaaagg atacaacagg tttctcaagt cagtatggtt
      541 ggattcattc ttttgcacat ggagccgatt tactgacaga ggtggtctgt catccagact
      601 ttcctataaa cagaattcat gaagtatttg acatacttgg ccaactattt aaaagaatgt
      661 cgattcgctt tacagatgat gaggattggc gtttagcaag agtgatctat gaacctattt
      721 tacaagggaa gttggagcaa gaacaagtag cttcttggat aaaaactgtt gattttccga
      781 tagaagaaag ggaggatttt tacaaatttt ccaactttag atcctgtctg gtggaagtct
      841 atgtccaact tgaccagaga aatagtttac aagatgacct gaaagaagct atccagtctt
      901 ttcaatacta gggattagct aatagttcta tgattttcaa aaaatttcca atcaattttc
      961 ttgaaaccct ttcctacttt tgatagacta gtatctagtt tttgaaaaaa ggagaaagaa
     1021 aatgaaaaaa tttgttgctg agttaatcgg tacgttcatg cttgtgttcg ttgggacagg
     1081 agctgttgtt tttggaaatg gttttgatgg ccttggtcac cttggaatcg cctttgcctt
     1141 tggtttggca atcgtggtgg cagcctactc aatcggaact gtttcaggtg ctcacttgaa
     1201 cccggctgtt tcgattgcta tgtttgtaaa caaacgtttg tcatcttcag aacttgtaaa
     1261 ctacatcctt ggtcaggttg ttggagcttt catcgcttct ggcgcagtct tcttcctctt
     1321 gggtaattca ggtatgtcaa ctgctagcct tggtgaaaat gccttggcaa acggtgtcac
     1381 tgtctttggt ggtttcttgt ttgaagtcat cgcaactttc ttgtttgtct tggttatcat
     1441 gactgtgact tcagaaagca agggcaatgg cgcgattgct ggtttggtaa tcggtttgtc
     1501 attgatggcg atgattcttg tcggattgaa gattactgga ctttcagtaa acccagctcg
     1561 tagcttggca ccagctgtct tggtaggcgg cgcagccctt caacaagttt ggattttcat
     1621 ccttgcacca atcgctggtg gagttcttgc agcccttgtt gcaaaaaatt tccttggaac
     1681 agaagaataa ttgaaactca aaaagccttg ctcctcatct tgaggaacag ggctttttcg
     1741 tatgatactc ttcgaaaatc tcttcaaacc acgtcagctt caccttgccg taggtatggt
     1801 tactgacttc gtcagtttca tctacaacct caaaacactg ctttgagcaa cctgcggcta
     1861 acttcctagt ttgctctttg attttcattg agtatgagtt tagcggttgt caattttctc
     1921 tggataaagg tcgtgttgga agaggcgttg ttctgccaag ccctcatact tagttccttg
     1981 cttaccgtag ttatagtagg ggtcgattga aatgccaccg cgcggagtga attttcccca
     2041 gacttctaaa tagcgagggt ctagcaagtt gaccaagtct ttcccgatgg tgttgataca
     2101 gttttcgtgg aaatctccgt ggtttcggta gctaaataga tatagtttga ggggttttga
     2161 ctcgacacag agcttgtcag gaatgtagga aatatgaatc gtcgcaaagt ctggctgagc
     2221 agtgatttgt cccagcagag acatatcgag gatatggtga cgaatgccct gttccttagc
     2281 gatttctcta gcaatttgaa tttcgaggtg atgacgttgg ccgtaggcaa aggtgacagc
     2341 ttcgactgtt tcatagtgtt gcatggccca gaaaaggcag gttgttgaat cttgaccacc
     2401 actaaagacg accaaggctg attgacgttt catagtactc cttccaaaat gggaaatgtt
     2461 cagagcacgc aaaaagctcc cattagggag ctaaaaaata ccaaatcgag gtttttttag
     2521 cgatggcata tcccaaacat cgtaatattc tacttatata gtaaaatgaa ataagaacag
     2581 gacaaatcga tcaggacagt caaatcgatt tctaacaatg ttttagaagt agaggcgtac
     2641 tattctagtt tcaatatact atagtctagc atattttttg aaaaatagca aagggcaaga
     2701 aaaaagagac caaagaaagt acttggtctc tcgtttgatt agctcaattc agcaatgatg
     2761 gccttgattt gttctgctgt gtgaacacct gcaacttgtt tgacaacttg gccgtctttt
     2821 ttgaagagaa gagttggaat agacatgatt ccaaaagcac gagctgtgtt tggattttca
     2881 tcaacgtcca ttttaacgat tttcaagaca tcttctgaaa gttcttcaga caatttgtcc
     2941 aagattggac cttgcatacg acatggacca caccaagttg cccagaagtc tactaagacc
     3001 aaaccgtctt ttgtttcttg ttcgaatgtt gcatctgtaa ttgcttttgc cattgtattt
     3061 ctcctttttt tagttatatt ggcttaaatc ttgtttcatg agatagaaga agatatctcc
     3121 ataagtccca tggtagtcca aattatgacc cttgtaagtt aatttttgga cagggtagta
     3181 gtctgcgacg ccgataaggc aagcttgttg cgaacgttca aagtcttcat aagactcgaa
     3241 agtcacagtt ctttcgttct tgctggcatc tatataggta atttcaatca tatttaaaac
     3301 tcctttgttt aatgctaact ttattttact ccttataaaa gagaatgtca agaaaaatga
     3361 ttgcgcacgc aacttttttt aaaaatcatc ttaaatcaag aaatccaaac ctgcttccaa
     3421 gctttcttcg acagtttttt gtagcgaggc cagtgtcttt tgcccatcat ttgtcaggca
     3481 gataaaacta gagcgtctat cttgatggca acacatgcga ctgagtagac cgcaattttt
     3541 agcttccaag cgagccacca tcctagaaac tgcgctcggg ctcagatgaa gtttatctgg
     3601 caggtcaatc tggcgtagag atttttcttc agccaagtcc agatagtaga gcaggtagaa
     3661 ctctttcaag gtcagacttt gctcgctctg ttgggcaatg gtctcttcca agagactttc
     3721 aatttctttc tgacgccgat tgaagtcaaa ccatttttcc aaataggtca tagtgtctcc
     3781 tttcttttta gagtcataat atagaagaaa gtccattaac gggcagtctc tgcgtcacaa
     3841 gatgattgcg catgcaataa ttatactact tttcaagaat gctggcaagc tctgtttttt
     3901 agtggttttc ttttttactg tccatatttt tggtaaaaat caacttttac ttggatgaag
     3961 gttttggctt cacgtaggag ttgaagaaga gtggcgcggg tttcaaactc ttctcttgtc
     4021 ttgggcagac tgcggttccg gaagacttcc agataacgtt caatttcatc tagcaaatca
     4081 gaagcaggat tggtctggct cagttgacct gcaatttttg aaaagagttg cgctaagatc
     4141 aggctttcac tggcggcaag gtgacaagtg ttaatctgtt gggccatgtt tctcaggata
     4201 cgactttgtc gctgtctcat ctcaaagtag tggatatggt agtcggtctg gtgaaagagg
     4261 tggtcagagt gatccaaata gaccagtctg agggcttctt tcaaaagcgt gtctaattct
     4321 gctaccagct gtgctcggtt gcgtccgtct cctctggata aatagtattt gaagcgctgg
     4381 aggatatctt ttaacttttc ttccaccagc gtgtggtagt gctggatttt ctcttctcgt
     4441 gaaggcatat agagattaac aagcaaggca aatcctgtac caatagcaaa gagaaggaat
     4501 tcattgacta gaaggtctgg agaggttgac tcttgaacca agagatggct aaccaaaaca
     4561 gtgcttggtg tgatgccaat ttcccagccc atcttgtagg ctaaaggaac gtagaaggcc
     4621 agatagaggc cgagactcca gatatgaaat ccgctcaagt gaaaagctag aacaccgata
     4681 gccagagcta gaagcataga aaaaagacga ttgcgagcca gttttaaagt acttctacgc
     4741 gtatcagata ggctcaagag agcgataatt ccagccgaaa ctgctgacga aagattgaga
     4801 aaataagcaa gcaggcaggc aagacaggta gctaagatga gcttggtcgt acgttggcta
     4861 atagacataa gaatttccta ataagttaga ataaaagcgt aaaagacaag acatgagcag
     4921 gcttgccttg atgagttatt ttttacgggt tgctgcgtat tcggcaacgg cggtaaagag
     4981 gacatctgta gaagagttaa gggctgtttc acatgagtct tggatgacac caatcacaaa
     5041 accaacccca acaacttgta tggcaatatc gttagaaata ccgaaaaggc tacaagcaac
     5101 tgggataaga aggagggaac ctccggcaat acctgaagca ccacaggctg agatagctgc
     5161 taccacactg aggacaaagg ctgtggcaaa gtcaacagga attccaagag tgttaactgt
     5221 aacaagggtc aaaacgttaa tggtaatcgc tactccagcc atattgatag tagaaccgag
     5281 tgggatagaa acagaatagg tatctgggtt gagtccaagg tcatggcaga gtttcatgtt
     5341 gacaggaatg ttagtcgcag aactacgagt gaaaaaggct gtcacaccgc tgacacggag
     5401 gcagttccaa actagagagt aaggattgcg tctcataaag aagaaggcaa tcaaagggtt
     5461 gaccacaggg gcaacaaaaa gcatagtcgt tactaatgga accaataaaa taccgtagtt
     5521 ggcaaggctt ccgactccct tgtcagaaat ggttttaaaa acaagaccaa ggattccaaa
     5581 tggagccaga ttgatgatcc attcgacaat tttagaagtc acgtcagcga tagtttttag
     5641 caattcttta ctatttttac tggttttact ggcttctctc atagcgattc caaaaatgac
     5701 tgcccaagat aagattccaa tatagttagc agtaagcagg gcgttgactg ggctgtcaac
     5761 cagtttgagc aagaggttgc tgaggacctg cccaatccca tctggtggtg caatttcagt
     5821 attagcacta tttagggtaa tttcaatagg gacgatgaaa cttgctagta cagctacaag
     5881 agcagcgtcg aaagtctcta tcataggata tacaagaaaa caacagtttt catattgcta
     5941 tcttgtccct tttgatgttg ggaaagggca ttggcaacga gggcaaagac taggatagga
     6001 gcaacagctt ttagacctcc aatgaataaa tccccgagta gcccaatccc tgagagatta
     6061 ggaagggtca gttctaggat tccccacaaa gcataccaat caagatacgc ttgacaaggc
     6121 ttgccttatt ccaagcatga atgattcttt tcataataat ctcctttttg tgtagtgatt
     6181 atgattatag tataaatgat agacaaaatc aagaattttc tgtctatttt ttgaatattt
     6241 atggagaatg agactgatga aaatatggta taatgaaata aaggagtttt atatgcaaaa
     6301 atttattcag gcttatattg aaaagctaga tgtgacaagc attatcgaga atattctaac
     6361 caaggtcatt tctcttttac tgcttttgat tgtattttat attgctaaaa aaatgcttca
     6421 taccatggtg cagagaattg tcaaaccttc tctaaaaatg tctcgtcatg atgtcggacg
     6481 ccaaaaaacc atctcacgtt tactagaaaa tgtgtttaat tatacgctat atttcttttt
     6541 actctactgc attttgtcga ttttaggttt gccagtttct agtttgctgg ctggagctgg
     6601 tattgctggg gtagcgattg gtatgggagc ccaaggcttt ctgtctgatg tcattaatgg
     6661 cttttttatc ctctttgaac gtcaactgga tgtgggagat gaggtcgttc tgacaaatgg
     6721 accgattact gtatcgggta aggttgtcag tgtgggaatt cgtacgacac agcttcgtag
     6781 cgaggagcaa gcccttcact ttgtccctaa ccgaaatatc acagttgtta gcaatttctc
     6841 acgcacagac tagacctgtt attttaagta atttgtggta caatagaggg agtttaataa
     6901 ggagaaaaga tggttttaga aaagcagttg ggcaatggtt gtacctggat agacctagac
     6961 ctaggaaagt tgaataaact agaagacctt tctgaaattt acggtttgga caaggaaacc
     7021 attgaatacg cactggatag aaacgagcgc gcccacatgg actaccaccg tgaaagtggg
     7081 acggttacct ttatctataa tgtcctagac gtaaaaaagg acaaggccta ctatgagact
     7141 tttcccatga cctttatcgt cgagcatcgt cgcctgatta ccattagtaa taccaagaac
     7201 gcctatgtca ttgaacagat gactcgttat ctggagaacc atgacacgct ttcgatttat
     7261 aagtttctct ttgccagtct ggaaatcatc agcaatgcct actatcctgt cattgagcag
     7321 atggacaaga gtagggatga ggtcaatgac ctcttgcgcc agcgaactac caagaaaaac
     7381 ctctttgccc tgtctgattt ggagactggt atggtttatc tgacggcagc tgccaaacaa
     7441 aatcggattt tgttagagca tattcaaggt catgccttgt atcgtagttt tgatgagatt
     7501 gagagagaac agtttgatga tgccatgatt gaggctcatc agctggtatc catgacagac
     7561 ctaatctctc agattttaca gcagctttca gcctcttaca acaatattct aaacaataat
     7621 ctgaatgaca atttgacaac cttgactatc atttcagtct tgctagctgt tttggcagtc
     7681 gtgacaggct ttttcggaat gaatgttccc ttacctttaa cagatgagcc ccatgcttgg
     7741 ctctatatca gtttggctag tgcaggtttg tggattgttt tatccttgtt actaaggaaa
     7801 attgcgaaaa aaagttaaga aaaggagcca gaatggcgat tgaaaattat ataccagatt
     7861 ttgctgtgga agcagtctat gatctgacag tcccaagcct gcaggcgcag ggaatcaagg
     7921 ctgttttggt cgatttggat aataccctca ttgcttggaa caaccctgat ggaacgccag
     7981 agatgaagca atggctacat gaccttcggg acgcgggtat tggcattatc gtagtgtcaa
     8041 ataacaccaa aaaacgcgtt caacgagcag ttgagaaatt tgggattgat tacgtttact
     8101 gggccttgaa gcccttcaca tttggtattg accgtgctat gaaggaattc cactatgaca
     8161 aaaaggaagt ggtcatggtt ggcgaccagc tcatgacaga tatacgagca gcccaccgtg
     8221 cagggattcg gtcaatttta gtcaaaccct tggtccaaca tgactcaatc aaaacgcaga
     8281 ttaaccgaac tcgtgagcgt cgtgttatga gaaaaatcac tgaaaagtac ggaccgatta
     8341 catataaaaa aggaatttaa ctatggaaga aattctctgt attggttgtg gagcaaccat
     8401 tcagacgaca gataaagctg gtcttggttt taccccccag tcggcacttg aaaaaggttt
     8461 ggagactggc gaagtctatt gccaacgctg tttccgtctc cgccactaca atgaaatcac
     8521 agatgtccag ttgacgaacg atgatttcct caagctcttg cacgaggtgg gagacagtga
     8581 tgctttagtg gtcaatgtta ttgatatctt tgattttaat ggatctgtca tcccaggttt
     8641 accacgtttc gtctcgggca atgatgtcct cttggtagga aataaaaaag atatccttcc
     8701 taagtcagtt aagtctggta agattagcca gtggctcatg aaacgtgcct atgaagaagg
     8761 tcttcgtcca gtcgatgtgg tcctaacttc agcacaaaat aaacatgcca ttaaggaagt
     8821 cattgacaag attgaacact accgtaaggg ccgcgatgtc tatgtggtcg gtgtgaccaa
     8881 cgttggaaaa tcaactctaa tcaatgctat tatccaagaa atcacgggtg atcagaatgt
     8941 catcactact tcacgcttcc cagggacaac cttggacaaa atagagattc cgcttgacga
     9001 cggatcttat atttacgata cgccgggaat tatccaccgc caccagatgg ctcactactt
     9061 gacggccaaa aacctcaagt atgtcagtcc taaaaaggaa atcaagccta agacctatca
     9121 gcttaatcct gagcaaaccc tatttttagg tggtttggga cgctttgact ttatagcagg
     9181 agaaaagcaa ggatttactg ctttctttga taatgaactc aaactccatc gtagcaagct
     9241 tgaaggagct agtgctttct acgataagca cctgggaact cttctgacac caccaaatag
     9301 caaggaaaaa gaagatttcc caaggctagt ccagcatgtc tttaccatta aagataagac
     9361 agacctagtc atttcaggcc taggctggat tcgtgtaaca ggcatagcaa aagtcgccgt
     9421 ctgggcacca gaaggcgtcg ccgtcgtcac acgaaaagca attatttaag cacagaaagg
     9481 aaagggttgt ctgaatttgg gcgagcaagg cgagccccat agagaatact tttcgctgtg
     9541 gtgtaagttg gtacaagtga ttgtaccaac tgcggaaaat ttgagacctt aggctcaaat
     9601 tttagtcatg aaagtccgaa ggactttgct gacgtccgtc accacttcag aaaagtataa
     9661 aaagaaactc ttttaaagaa attatgtcat taacatcaaa acaacgtgcc ttcctcaaca
     9721 gccaggcaca caccctcaaa cctatcatcc aaatcgggaa aaatggactc aacgaccaaa
     9781 tcaaaaccag cgtccgtcaa gctcttgatg cgcgtgaatt aatcaaggtt actctcttac
     9841 aaaacacaga tgaaaacatc cacgaagtag ctgaaatttt ggaagaagaa atcggtgtgg
     9901 atacagtcca aaaaatagga cgcatcttga ttttgtttaa acaatctagc aagaaagaaa
     9961 atcgcaagat ttctaagaaa gtcaaagaaa tctaagaccg aaactccaaa taactgtttt
    10021 tatagagaaa taaaggggac tagcctatga caatcgaact attgactccc tttaccaagg
    10081 tagagttgga gccagaaatc aaggagaaaa aacgcaaaca agttgggatt ttagggggga
    10141 attttaaccc tgttcacaat gcccatctca ttgttgcgga tcaagtacgg caacagttgg
    10201 gactggatca agttcttctc atgcctgaat accaacctcc tcacgttgat aaaaaggaaa
    10261 ccatccctga acaccatcgt ctcaagatgc ttgagttggc aattgaggga attgacggcc
    10321 tagtcattga aaccattgag ttggagcgca agggtatttc ctacacctac gacaccatga
    10381 agattttgac agagaagaat ccagatacgg attattactt tatcatcggt gccgacatgg
    10441 ttgactatct gcctaagtgg taccgaattg atgaactggt tgacatggtt cagtttgtgg
    10501 gggttcagcg tccacgctac aaggcaggga cttcctatcc agttatctgg gtggacgtac
    10561 cgctcatgga tatctcgtcc agcatggtgc gggacttcat tgcccaaggt cggaaaccca
    10621 actttctcct acctcagcca gtgctagact acatcgagaa ggaggggctc tactgatgtc
    10681 ctatcaagac tatatcaact gctcccgtga ggccttgttg gaaaaaatgg cagagcttct
    10741 acctgagaaa cgtctaaccc attgtctagg tgtggagcgt gccgctatgg aattagctca
    10801 gcgctttggt gtagatgttg agaaagcaag tctagcaggt cttcttcatg actatgctaa
    10861 aaagttgtca gatcaggaat ttctagactt gattgaccgt tatcagctag atttcgacct
    10921 caaaaactgg ggtaataatg tctggcatgg tatggtcgga atttacaaaa ttcaggaaga
    10981 tttggatttg catgattctg aaattctgcg agccattgaa attcatacag tcggagctgg
    11041 tcaaatgaca gacctagata aggtcatcta cgtcgcagac tatatcgagc acaatcgggc
    11101 atttcctgga gtggatgtgg cgcgtgagat tgcaagtcta tcgctcaata aggcggtggc
    11161 ctacgaaaca gctcgtaccg tggagtatct agctcatcag ggattcccca tctatcccca
    11221 aacccttgaa acctataacg cctttgtgca ctatttgaaa gaggactaaa tgaagacggc
    11281 ttttataatc attgatgttc agaatgtttt agtagaaact gggttccaga caaaaagtct
    11341 attggaaaaa atttcttatt tacaaaacca ggctagaagc aagaatattg aaattatcta
    11401 tgttcaacat attgagaact ctgaagctca aacatcagaa gattggcagt tatctgcgct
    11461 tttaaatcga aaacctgctg aaaaggtctt tcagaagaag tataacagta ttttcaaaga
    11521 aactggctta aaagaatact tggataagca ggggattgaa aaattagttt tatgtggtat
    11581 gcagacagaa tattgtgtgg atacctctgt caaggttgcc tttgaatatg gctatcagct
    11641 tattgttcca gaaggtgctg tcacaacctt tgatggggat gacattccag cagaaacgat
    11701 taatgaattt tatgaggaca tttgggagga gcgctttgca gatgttctag attacaaaca
    11761 tattttctaa aaagaggatt aaatgaacga aaaagaatta ctagaactag tcgtgaaagc
    11821 ggctgatgag aaacgtgcgg aggatatcct cgcacttgat gtacaagatt tgactagtgt
    11881 gacggactac tttgtcatca ctagctcaat gaatagccgt cagttggacg ctatcgcagc
    11941 taatatccgt gaaaaagtag ctcaagcagg ctttaaaggt agccatgtcg aaggcgatgc
    12001 agctggaggc tgggtcttac tggacctcgg tgctgttgtc gtgcatatct tttcagaaga
    12061 aatgcgtgcc cattataatc tagagaagct atggcatgag gcgaattcag tagatatttc
    12121 agaagctctt gcttagaaga tgaactcagt tgtagtcaac tgggtttctt tgcttatttt
    12181 agaaaaattg atagaaaggt aaagggctag tggtgtttac catggagctc ttagtatcat
    12241 gattatggcg acttatgaaa cctttgcggc ggtctatgat gcggtcatgg acgatagttt
    12301 atacgacaaa tggacgaatt tttctctgcg tcatttgcct aagaccaagg agagaaagaa
    12361 actcttggaa ttggcttgtg ggacaggaat tcaatcagtg cgcttctctc aggctggttt
    12421 tgatgtgact ggacttgact tgagtgcgga tatgttgaag attgcggaga agagagcaac
    12481 ttcagccaag caaaagattg cttttataga aggtaatatg ctgaatttgt ccaaggcagg
    12541 gaaatacgat tttgtcacgt gttattcgga ctctatctgc tatatgcagg atgaggtgga
    12601 agtaggggac gtctttaagg acgtgtacaa tgcgctgaat gaagagggag ttttcatctt
    12661 tgacgtgcac tcgacctacc agacagatga agtattccct ggctattcct accatgaaaa
    12721 tgcggaagat tttgccatgc tttgggatac atatgaggac gaagctcctc actccatcgt
    12781 gcatgagctg actttcttta tcaaggaggc tgacggttcc tttagtcgcc acgatgaagt
    12841 acatgaggag aggacttatg agatcttgac ctatgatatc ttgctggaac aggctggatt
    12901 caagtccttc aaactctatg cagactttga ggataaggaa ccaacagaaa ctagcacccg
    12961 ttggtttttt gtggcgcaga agtaggagaa catcatgacc atcacaggta ttatcgcaga
    13021 gttcaatcct tttcataatg gccataaata cctgctggat caggcagagg gactgaaaat
    13081 tgttgccatg tcagggaatt tcatgcagcg tggagagcct gctattgtgg ataagtggac
    13141 aagggcccag atggcgctgg aaaatggcgc agacttggta gtggaattgc cctttttagt
    13201 cagtgttcag gcagctgatt tctttggtca aggagctgtg gatatcttgg atcgcttggg
    13261 cattgatagt ctagtttttg ggacagagga agttcgagat tatcagaaaa tcgctgactt
    13321 atacacagag aaaggtgctg agatggagaa atttgtggaa aatctgcctg attccctttc
    13381 ctatccccag aaaacccaat ctatgtggaa ggaatttgct ggtcttgatt tttcaggtaa
    13441 tacccccaat catgttcttg ctctggccta tgcaaaggcg gttgcaggac gaaatatcaa
    13501 gctgcatacg attcagcgtc agggggcagg ttaccattct gtgaataaag atgtggactt
    13561 tgcctcggcg acagccctcc gtcaacatca gaaggaccaa gatttcttag aacgctttat
    13621 gccttctgtt gccctatttg agcaggccag taaggtgatc tgggaagact attttccctt
    13681 gctccgctat caaatcttgt caaatcccga cctaaccacc atctatcagg tcaatcaaga
    13741 aatggctgtg cgcattaaag aagctattaa aacagcccag tctgtagaag agttggtcga
    13801 ggcggttgcg accaaacgtt acaccaaggc gcgtgtcaga cgcctattga cctatatttt
    13861 ggtgcaggct agagaaaatg tcttgccaga agccattcat gtccttggat ttactgaaaa
    13921 aggcaggcaa catctcaagt ctctgaaagg acaggtcaat ctagtcagcc gaattggcaa
    13981 agaaccttgg gatgctatga cccaaaaagc agaccagatt taccaactag gaaagccaag
    14041 tatagcagag caaaattttg gaagagtgcc gattagaata gaaacaagct aagtctaccg
    14101 aaaggtaggc tttttgagat tttttacgaa taatactctt cgaaaatcaa attcaaatcg
    14161 cgtcaacgtc gccttgccgt actcaagtac agcctgcggc tagtttccta gtttgctctt
    14221 tgattttcat tgagtataaa tagatagaaa agaaaatctt gtacaagttc ctgacaattt
    14281 ctttcttttc gtgtataata gtggaaaaga gctaaaacga ggtatggtta tggaagcagt
    14341 cctttactca acattccgaa atcatttaaa ggactacatg aagaaggtaa atgatgaatt
    14401 tgagcctttg acggtggtca ataaaaatcc agatgaggac attgtagttc tttcaaagag
    14461 cgaatgggac agtattcagg aaaccctgag aatcgctcaa aataaggagt tatcagacaa
    14521 ggttttgcga ggaatggctc aagtccgtgc tggaagcatt caggtccatg tgattgagga
    14581 gtgatgaatg ctactcaagt ttacagaaga tgcgtgggca gattattgct actggcaaaa
    14641 ccaagataaa aaaacactaa aaagaatcaa taaattaatc aaggatattc aacgcgatcc
    14701 atttacagga ataggaaaac cagagccact caaatatgat taccaaggag cctggtcacg
    14761 gcgtattgat gcagaaaatc gcttgattta tatgatggat ggagatagcg tggctttctt
    14821 gtcctttaaa gatcattact aagtctattg aaaggtaggc tttttctcaa gtctctacta
    14881 aactactatt tctaaaaaac aagtttattt tggcaaatgt gttccaaatt ccccaaaaca
    14941 tattctattt taggtttgtg aaaatcactt ttttatggta gaatgtaaaa gaatgtatgt
    15001 cattcggaat aacaataaaa tgaggtaaaa acatggaaat catgtcgctt gcgattgctg
    15061 tttttgccgt catcattggt ttagtcattg gatatgtcag catctcagct aagatgaaat
    15121 catctcagga agctgcagag ttgatgcttt taaatgctga acaagaagca actaatttac
    15181 gtggacaagc tgagcgtgaa gcggatttac ttgttaatga agccaaacgt gaaagcaagt
    15241 ctcttaaaaa agaagcacta ttggaggcca aagaagaagc cagaaaatac cgtgaagaag
    15301 tggacgctga attcaaatca gaacgtcaag aactcaaaca aatcgaaagt cgtttgacag
    15361 agagagctac tagccttgac cgtaaggacg acaatttgac gagtaaagaa caaacacttg
    15421 aacaaaaaga acaaagtatt tctgatagag cgaaaaacct tgatgcgcgt gaagagcaat
    15481 tagaggaagt cgaaagacaa aaagaagcag aactagagcg tattggtgct ctgtctcagg
    15541 cagaagcacg agatattatc ttggctcaga cagaggaaaa cttgaccagg gagattgcca
    15601 gtcgcattcg cgaagctgag caagaggtca aggaacgttc tgacaaaatg gccaaggaca
    15661 tcttggttca agctatgcaa cgtatcgctg gtgaatatgt agcggagtca acaaactcaa
    15721 cagttcatct gccagacgat actatgaagg gacgcattat tggtcgtgaa ggtcgtaaca
    15781 ttcgtacctt tgaaagtttg acaggggtcg atgtgattat cgacgataca ccagaagtgg
    15841 tgaccttgtc aggatttgat ccgattcgtc gtgagattgc ccgtatgact atggaaatgt
    15901 tgctcaaaga tggtcgtata catccagctc gtatcgaaga gttggttgag aaaaaccgtc
    15961 aagagattga caataagatt cgtgaatacg gtgaggctgc tgcctatgaa attggtgcgc
    16021 caaaccttca tccagacttg atgaagatta tgggacgttt gcagttccgt acttcatatg
    16081 gacaaaatgt tttgcgccat tcgattgagg ttgctaagtt ggctggtatc atggcgagcg
    16141 aacttggtga aaatgcggct cttgcccgtc gtgctggatt ccttcacgat atcgggaaag
    16201 ccattgacca tgaggttgaa ggtagccacg ttgaaatcgg tatggaattg gcccgtaagt
    16261 acaaggaacc cccagttgtg gtgaatacga ttgctagtca ccacggagat gttgaagctg
    16321 agagcgtgat agcagttatc gtcgctgcag cagatgcctt gagcgcagcc cgtccaggtg
    16381 ctcgtagtga gtctcttgaa agctacatca agcgtctcca tgatttggaa gaaattgcta
    16441 acggctttga aggagtgcaa actagctttg cccttcaagc aggacgtgaa attcgtatca
    16501 tggtcaatcc aggaaaaatc aaggacgaca aagtcacaat cttggctcac aaagttcgta
    16561 agaaaattga aaacaatctc gattatccag gaaatatcaa ggtaaccgtg attcgcgagc
    16621 ttcgtgcagt agattatgct aaataaataa gaaagagcag ttgaaaaatt actgcttttt
    16681 tgttacacta gatagaaaga ctgtaaagga taatctggct ttttgccatt attctagaga
    16741 aatgttattt cacagattga ctaaaaggag acacaatggc agaccgaggc ttactaatcg
    16801 ttttttctgg tccttcaggg gttggaaaag gaacggttag aagagagatt tttgagagtt
    16861 ctgaaaacca atttcaatat tctgtatcga tgacgacacg cgcacaacgt cctggagaag
    16921 tggacggtgt tgactatttc ttccgtactc gtgaagaatt tgaagagctg attcgtcaag
    16981 gacagatgtt ggaatacgca gaatatgtcg gcaactacta tggaactcct ctgacctatg
    17041 tcaatgaaac cttggacaag ggaatcgatg ttttccttga aattgaagtt cagggtgctc
    17101 ttcaggtcaa gaaaaaggtt ccagatgctg tctttatctt cctgacacca ccagatttgg
    17161 atgaattgca agatcgcttg gtaggtcgtg gaacagatag tgcagaagtg attgcccaac
    17221 gaatcgaaaa ggccaaggaa gaaattgccc tcatgcgtga gtatgattat gcgattgtca
    17281 acgatcaggt gcccctagct gctgaacgtg tcaaatgtgt gattgaagca gaacacttct
    17341 gtgtggatcg tgtcattggt cactatcagg agatgttacc aaaatctcca actacccgat
    17401 aaaatttaga aaataggtac aagcaaatga tgttaaaacc ctctattgat accttgctcg
    17461 acaaggttcc ttcaaaatat tcactcgtaa tcttggaagc aaaacgtgcc cacgaattgg
    17521 aagcaggtgc cccagcaact caaggtttca agtctgaaaa atcaactctt cgcgctttag
    17581 aagaaatcga atcaggaaac gttacaattc acccagatcc agaaggaaaa cgtgaagcag
    17641 tgcgtcgccg tatcgaagaa gaaaaacgcc gcaaagaaga agaagaaaag aaaatcaaag
    17701 agcaaattgc taaagaaaaa gaagatggtg aaaaaattta aggttggggg gactcaatct
    17761 tattttttct attgcaagaa tgtactgaca aggaggaggt gagaagatgg ccctagctaa
    17821 gattatcgta gatgtgccct tgatgcagac ggaccagccc tatagttaca ggattccgga
    17881 ggaatttgag ggaatgctgg aagttgggat gcgggttcat gtgccttttg gtaagggtaa
    17941 tcgcctgatt caagggattg ttcttggttt ggagtcccaa tcagatggag aagagatgga
    18001 gcaagattta aaagatattg cagaggtgct ggatttttct cctgttctca cgccagaaca
    18061 actctggctg gctgaggagt tacgcaaatc tgtcttctcc tacaaaatct ctatcctcaa
    18121 ggccatgctt ccgggatttc tgaattctag ctatgacaag attctctatc ctctggaagg
    18181 cttgagtcag gaagaacgag tacgcctgtt tgattcagaa gattcgctag ccttttcttc
    18241 tctggattta ggtaagcaag ctgaaatgat gcgtttgact agaaaaggcc tgcttggtct
    18301 ggaatatcag gcagtcgatc aaaagaaggt caagacccag tcttggtatg aggttgacca
    18361 tgctcaatta gaaggtgttg agatttctac acgtgccaag aaaaagttgg aactgagaga
    18421 ttatctgctg tctcatccag agagcgcttc cttggctagc ttgttagagt cctactcgcg
    18481 agagcaagtt aacttctttg tggatcaagg tgctgttact atagtccaaa aggaagttcg
    18541 acgctcggct gcttattttg aaggaattga agcaagtaga cctttggagt taaatccaga
    18601 acaaagacag gcgcgtgatg cggttgtgag ttctattggc agttctcaac ctccctttct
    18661 acttcaaggg attacaggaa gtgggaagac cgaggtttac ttgcagatta tccaaggtgc
    18721 cctggataag ggcaagacag ctattttgct ggtacctgag atttccttga ctcctcagat
    18781 gaccgagcgt tttatagcac gttttgggga caaggtagcc attcttcact caggattatc
    18841 caatggtgaa aagtatgatg aatggcgcaa ggtggagcgt ggcgatgccc aagttgttgt
    18901 tggtgccaga tctgctatct ttgctcctct gaaaaatctg ggtgtcatga ttattgatga
    18961 agagcatgaa gcggcttata agcaggacag caatccccgt taccatgcca gagaggtagc
    19021 tattttacgg gctcagtata atcaagcaac tctggtactt ggatctgcaa cgccgagctt
    19081 agagagccgg gcacgtgctg gcaaaggcgt ctatcaacac ttacgtctaa cccaacgtgc
    19141 caatcctttg gctacaatcc ctgaggttca agtgattgac tttcgagact atatcggaca
    19201 aaatgagacg tcaaacttta cgcctccttt gctagaggct atccaagacc gtctggttaa
    19261 aaaagagcag atggttctca tgctcaatcg ccgtggttat tctagctttg tcatgtgtcg
    19321 ggagtgtggg acggtggata cttgtcccaa ctgcgatatt tctctgacct tgcatatgga
    19381 taccaagact atgaactgcc attattgtgg tttctcgaag gatattcctc aggtctgtcc
    19441 taactgtaag agccacagta ttcgttacta tgggacggga actcagaagg cttatgatga
    19501 gctagcagaa ctctttcccc aagctcgcat tttgaggatg gatgtggata cgactcgaaa
    19561 gaaaggcagt caccaagcct tgcttgacca gtttgggcga ggggaagcgg atattttact
    19621 tggtactcag atgattgcaa agggtttgga ttttcccaat gttaccctag tcggagttct
    19681 aaatgcggat acggccttga atctgcctga tttccgttct tctgagagaa ccttccagat
    19741 cttgactcag gtggcaggtc gagcaggtcg ggctgaaaaa gcaggtcaag tcttgataca
    19801 gtcctacaat ccgcagcact atgctattcg atttgccaag gatcaagatt acgaaggctt
    19861 ttatgcctat gaaatgggaa tcagacgaca actcggctat ccaccttact atttcacgat
    19921 tggtattacc ctttctcaca agaaagaaga agaggttttc aaacgtgcct atgaagtcat
    19981 gaacattttg cggtcaggct tgtcagagac cagtcccatt ctggggccaa cgccaaaacc
    20041 gattgcccgt acccacaacc tctatcatta ccagatttta attaaatacc gtttagaaga
    20101 tgagctgggg ccgaccctca accaggtcct ggccttgact caagaacggg aaaatagtga
    20161 gctccgtctc agcattgacc atgagccgca gcaattttta taagaaggag aagatatgac
    20221 aaaactaatc tttatgggga cccccgactt ttcagcaaca gtcttaaaag gacttttgac
    20281 agatgaccgt tacgaaattc tagccgttgt gacccagcca gaccgtgctg ttggtcgtaa
    20341 aaaagttatc caagaaaccc cagtcaagca ggctgccaag gaagcaggat tatctatcta
    20401 tcaacctgaa aaattatctg gaagtccaga gatggaagat cttatgaagc taggagcaga
    20461 tggaattgtg actgctgctt ttgggcagtt tctcccaagc aaactccttg atagcatgga
    20521 ctttgctgtc aacgttcatg cctccctcct tcctagacac cgtggtggtg cgcctatcca
    20581 ttatgccttg attcaagggg atgaggaagc tggtgtgacc atcatggaaa tggttaagga
    20641 aatggatgca ggagatatga tttctcgtcg cagcattccg atcacagatg aggacaatgt
    20701 tggcaccttg tttgaaaaat tggcgctagt tggtcgtgat ttgcttttgg acactctgcc
    20761 tgcctatatt gctggtgata tcaaacctga accgcaggat acgagtcagg ttaccttctc
    20821 tccaaatata aagtcagagg aagaaaaact gaactggaac aaaaccaatc gtcaactctt
    20881 taaccaaatt cgtggaatga acccctggcc tgttgcccat actttcctta agggtgaccg
    20941 ctttaagatt tatgaagccc taccagtaga aggtcaggga aatccaggtg agattctctc
    21001 tatcggcaag aaagaattga ttgtcgcaac ggctgaaggg gctctatccc tcaaacaagt
    21061 gcagccagct ggtaagccta agatggacat tgcttccttc ctcaacggag ttggacgtac
    21121 attgactgta ggagaacgat ttggtgacta aagtagaaac ggctagaagt ttagctctag
    21181 cagtgctaga ggatgttttt gtgaatcaag catattcaaa tattgcctta aataaacacc
    21241 tcaaggggag tcagctctct gcagcagaca agggcttagt gaccgagctg gtctatggaa
    21301 cggtagcccg taaactgact ctggaatggt acctatccca ctttatcgaa gacagagacc
    21361 agttagacag ctggctctat gtccttcttc tcatgagtgc ctaccaactc cgctatttgg
    21421 acaagattcc agatcatgct gtggtcaatg aagcagtgga attggccaaa ctccgtaaaa
    21481 aaggcagtga aaaattggtc aacgctgtcc ttcgccgtat cttgcgtgaa ggctggccag
    21541 atattgctag catcaagcga aaaaacaagc gtgactccat tgcctattct ctcccagttt
    21601 ggctagttgc caaactcaag gaagaatacg gagaggagag agcaaaagcc atctttgaaa
    21661 gcctgttagt gcgaaacaaa gccagtattc gtgtaacaga cctaagtcga aaagaggaaa
    21721 tccaagcctt gttggaggcg aataattccc ttttagcaac cactggtctg gttaaggagc
    21781 aagggcattt tgcagggcat aatttgttta cgaatggagc cattaccatc caagacgagt
    21841 ccagtcagct ggttgctccg acgcttgatt tacaaggtga tgagcgagtg cttgatgcct
    21901 gtgcggctcc aggtgggaaa acagcccata tagcctctta tcttacgaca ggtcaggtta
    21961 ctgctctgga cttgtacgac cacaagttgg atttaatcca agaaaatgcc caacgtctgg
    22021 gagttgcaga tcgggttcaa actcaaaaat tagatgccag aaaggtgcat gagttttttg
    22081 accaggattc ctttgataag attttggtgg atgctccttg ttcaggaatc ggtcttttgc
    22141 gccgaaaacc agatatcaaa tacaataaag aaacggcaga tttcgcgtcc ttgcaggaaa
    22201 ttcagttaga aatattaggt agtgtttgtc aaatactagg caaaggtggt ataataactt
    22261 atagcacctg cactatcgtc tcagaggaga attttcaagt cgttaaggcc tttttagaga
    22321 gtcatcccga gttcgagcag gtaaaactag aacatgaatg taaggatatc atgaaagacg
    22381 gctgtatcct cattacacct gaattgtatg gaagtgatgg attcttcatc agtcaatttc
    22441 gcaagatatc ggattaggaa ggaactgaca catggaaatt tcattattaa cagatgttgg
    22501 tcagaaacga acaaataacc aagactatgt caaccactat gtcaatagag ctggacgtac
    22561 catgattatt ttagctgatg ggatgggagg tcatcgtgca gggaatatcg ctagtgaaat
    22621 ggcggtcaca gacctgggtg tagcttgggt tgatacccag atcgatacag tcaatgaagt
    22681 gcgtgaatgg ttcgcccatt acctagaaat tgaaaatcaa aagattcacc agcttggtca
    22741 ggatgaagct tacagaggta tgggaactac tttggaagtc cttgctatta ttgataatca
    22801 ggctatctat gctcatattg gtgattcgcg tatcggcttg attcgtggag aagaatacca
    22861 tcagttgacg agcgatcatt ccttggttaa tgaattgctc aaggctggtc aattgacacc
    22921 agaagaggca gaagctcatc cgcaaaaaaa tattatcacc cagtctattg ggcaaaaaga
    22981 tgaaattcag cctgattttg ggacagttat ccttgagtca ggtgactatc tcttgctcaa
    23041 tagtgacggc ttgaccaaca tgatttcagg cagtgagatt cgtgatattg taaccagtga
    23101 tattccttta gcagataaaa cggagacact tgttcgtttt gctaacaatg caggaggttt
    23161 agacaacatt acggttgccc ttgtttctat gaacgaggag gatgaagaat gatccaaatc
    23221 ggcaagattt ttgccggacg ctatcggatt gtcaaacaga ttggtcgagg aggcatggcg
    23281 gatgtctacc tagccaaaga cttaatctta gatggggaag aagtggcagt gaaggttctg
    23341 aggaccaact accagacgga cccgatagct gtagctcgtt ttcagcgtga agcgagagct
    23401 atggcagatc tagaccatcc tcatatcgtt cggataacag atattggtga ggaagacggt
    23461 caacagtatc ttgcaatgga gtatgttgct ggactagacc tcaaacgcta tatcaaggaa
    23521 cattatcctc tttctaatga agaagcagtc cgtatcatgg gacaaattct cttggctatg
    23581 cgcttggccc atactcgagg aattgttcac agggacttga aacctcaaaa tatccttttg
    23641 acaccagatg ggacggccaa ggtcacagac tttgggattg ctgtagcctt tgcagagaca
    23701 agtctgaccc agactaactc gatgttgggc tcagttcatt acttgtcacc agagcaggcg
    23761 cgtggttcga aggcgactgt gcagagtgat atctatgcca tggggattat tttctatgag
    23821 atgttgacag gccatatccc ttatgacggg gatagcgcgg tgaccattgc cctccagcat
    23881 ttccagaaac ccctgccgtc cgttattgca gaaaatccat ctgtacctca ggctttagaa
    23941 aatgttatta tcaaggcaac tgctaaaaag ttgaccaatc gctaccgctc ggtttcagag
    24001 atgtatgtgg acttgtctag tagcttgtcc tacaatcgta gaaatgaaag taagttaatc
    24061 tttgatgaaa cgagcaaggc agataccaag accttgccga aggtttctca gagtaccttg
    24121 acatctattc ctaaggttca agcgcagaca gaacacaaat caatcaaaaa cccaagccag
    24181 gctgtgacag aggaaactta ccaaccacaa gcaccgaaaa aacatagatt taagatgcgt
    24241 tacctgattt tgttggccag ccttgtattg gtggcagctt ctcttatttg gatactatcc
    24301 agaactcctg caaccattgc cattccagat gtggcaggtc agacagttgc agaggccaag
    24361 gcaacgctca aaaaagccaa ttttgagatt ggtgaggaga agacagaggc tagtgaaaag
    24421 gtggaagaag ggcggattat ccgtacagat cctggcgctg gaactggtcg aaaagaagga
    24481 acgaaaatca atctggttgt ctcatcaggc aaacaatcct tccaaattag taattatgtc
    24541 ggccggaaat cttctgatgt tatcgcggaa ttaaaagaga aaaaagttcc agataatttg
    24601 attaaaattg aggaagaaga gtcgaatgag agtgaggctg gaacggtcct gaagcaaagt
    24661 ctaccagaag gtacgaccta tgacttgagt aaggcaactc aaattgtttt gacagtagct
    24721 aaaaaagcta cgacgattca attagggaac tatattggac ggaactctac agaagtaatc
    24781 tcagaactca agcagaagaa ggttcctgag aatttgatta agatagagga agaagagtcc
    24841 agcgaaagcg aaccaggaac gattatgaaa caaagtccag gtgccggaac gacttatgat
    24901 gtgagtaaac ctactcaaat tgtcttgaca gtagctaaaa aagttacaag tgttgccatg
    24961 ccgagttaca ttggttccag cttggagttt actaagaaca atttgattca aattgttggg
    25021 attaaggaag ctaatataga agttgtagaa gtgacgacag cgcctgcagg tagtgtagaa
    25081 ggcatggttg ttgaacaaag tcctagagca ggtgaaaagg tagacctaaa taagactaga
    25141 gtcaagattt caatctacaa acctaaaaca acttcagcta ctccttaaaa gcagatggat
    25201 aatcaaaatg agtaatcaat ctttgtcatg atttcatggc aaagattttt tttgagtcca
    25261 gatttgtgat agaatagagg gagttgataa aaggaggaaa gcatggaaga atcaaaagaa
    25321 ttaaatgccg tcattgatgt gattatgcta gcggggacta ttctccttaa aagtggctca
    25381 gaaatccatc gtgtagaaga taccatgatt cgaatcgcgc attcgcaggg gattgtggat
    25441 tgcaatgtcc ttgccatgcc tgccgctatc tttttctcta ttgaaaatac caatatttcg
    25501 cgcatgaagc gcgtgacctc ctcttcttat aacatcgaaa aagtctgcga tgtgaaccag
    25561 atttctcgtc agctagttgg ggggcagatt gacttagaaa cagcctttaa gcaattgacg
    25621 gccttgcaag ctcaacccct tccctatact aagttgcagg taactctggc tgcgaccttt
    25681 agtgctcctt tcttttcagt tatgtttagc ggaaatatct acgacgcact tggggcagga
    25741 gtggcgacct tatttggttt tgccttttcc ctctatgtgg aaaaatttat ccgaattccc
    25801 tttgtgacag cctttgctgg agcctttgtc tttgggataa ttgcccagtt ttgggctcgc
    25861 tacacaggtt ttccttcaac ggcagatttg attatagctg gtgcggtcat gccgtttgta
    25921 ccaggtattg ccttgaccaa tgcggtccgt gatattatga ccaaccacat aaactctggt
    25981 atgagtaaga tgtttgaatc cctgctcatt accctcgctt taggggcagg aacttctgtc
    26041 gccttggtat tgatgaacta atatgacact aacaaccttt ttattacaag cagtagcaag
    26101 ttttcttgcc attatcactt ttttaatcgt actcaatgtg caacggtcta tgctcttacc
    26161 tggagggatt ttgggcatga ctgtctggct aatctatctc ttgctcaagg aaccgaccaa
    26221 tgtcattgta gctaccttca ttgcagccat tattggttct tgtgtcagcc agattttaag
    26281 tattctttat aagacacctg ctgtggtctt tatcttggcc attttggcac cgctggttcc
    26341 aggttatctc tcctaccgaa caactgcctt ttttgtgaca ggggactata ataaagcact
    26401 ggcaagtgcg accttggttg tcatgttggc tttggtaatc tctattggaa tggctagcgg
    26461 aacagtgatt ctcagactgt atcattatat aaaaacacat cgagtatcgt agactttaca
    26521 gaaataaaag aattttctga aaaatgagat aaataaatta acaacgcttt ctatatgtgc
    26581 gagaataccg cacttatgaa gaaattgcgg ctgattttgg tatccacgaa agcaacttac
    26641 tccgtcggag ccaatgggtt gaagtaactc ttgttcaaag gtggtgttac gatttcaaga
    26701 actcctctca gttctgaaga cacggtaatg attgatgcga cggaagtaaa aatcaatcgc
    26761 cctaaaaaaa acaattagcg aatgattctg gtaaaaaaaa tgccacgcta tgaaggctca
    26821 agcgattgtc acaagtcaag ggagaattgt ttctttggat atcgctgtga actattgtca
    26881 tgatatgaag ttgttcaaaa tgagtcgcag aaatatcgga caagctggta aaatcttggc
    26941 tgacagtggt tatcaagggc tcatgaagat atatcctcaa gcacaaactc cacgtaaatc
    27001 cagcaaactc aagccgctaa cagttgaaga taaagcctat aaccatgcgc tatctaagga
    27061 gagaagcaag gttgagaaca tctttgccaa agtaaaaacg tttaaaatgt tttcaacaac
    27121 ctatcgaaat catcgtaaac gcttcggatt acgaatgaat ttgattgctg gcattatcaa
    27181 tcatgaacta ggattctagt tttgcaggaa gtctactact ttccttattg tctgtaaatc
    27241 tactgacctt gttgtttatc ccagtcatgg tttctagttc gggctcagag tttcaaagtg
    27301 gatggcaaga gcatcaattg attgctgaga aggttagtaa aacacttgac aagacatttg
    27361 ataaggatgt cagagaaatt ccgaccagtc agttttatca aaaatttgta gatgagatgg
    27421 gaaggactta ctcaggaaat ttgatcctcc aggagctgat aactgtgaat ggagcttata
    27481 aagctactta tatcggtgaa ctctctagca actaatcatt ctttaaaatg atgaccaata
    27541 agaggcttga tttggtgaat caagtctttt ttctctcttt tactgccatt tgtgataaaa
    27601 tagtatagaa cgatttttta catgaatgat aaaacagagg taaatatgac aatcggtatt
    27661 gataagattg gttttgcgac cagtcaatat gtcttgaaat tacaagactt agcagaagcg
    27721 aggggaattg accctgaaaa attaagtaaa ggactcttac tcaaggaatt gagtattgcg
    27781 cccctaactg aggacatcgt gaccttggcg gccagtgcta gtgactctat tttaactgag
    27841 caagaaagac aagaagttga catggtcatt gtggctaccg agtcaggaat tgaccagagt
    27901 aaggctgcgg ccgtctttgt gcatggcttg ctgggcatcc agccctttgc tcgtagtttc
    27961 gagattaaag aagcctgcta tggggcgaca gctgccctcc attatgccaa attgcatgtg
    28021 gaaaattctc cggagtccaa ggtattggtc attgccagtg atattgccaa atatggtatt
    28081 gaaactccgg gagaaccaac tcaaggtgct ggaagtgtag ctatgttgat tacacaaaat
    28141 ccacgcatga tggcctttaa taatgacaat gtagctcaga cccgtgacat catggatttc
    28201 tggcgaccaa attactcgac aactccttat gtaaatggtg tctattctac ccaacaatac
    28261 ttggatagtt tgaaaacgac ttggcttgaa tatcaaaaac gctaccagct tactttggat
    28321 gattttgcgg ctgtttgttt ccacttgcct tatcctaaat tagcgctaaa aggcttgaaa
    28381 aaaatcatgg ataagagcct gcctcaagag aaaaaagacc tcttacaaaa gcattttgac
    28441 cagtctattc tctacagtca aaaggtgggg aatatctaca caggttcact tttccttgga
    28501 cttttgtctc tcttggaaaa tacagatagc ttgaaagctg gggataaaat cgccctttat
    28561 agttacggaa gtggagctgt ggctgagttc ttcagtggtg aattggttga aggatatgaa
    28621 gcttatttga ataaagaccg tttgaacaag ctcaaccaac gaactgccct atccgttgca
    28681 gactatgaaa aggtcttttt tgaggaagta aacttggatg aaacaaactc tgcccagttt
    28741 gctggctatg aaaatcaaga ttttgccttg gttgaaattc tcgaccacca acgccgttat
    28801 agcaaggttg aaaaataatg aagataagtt ggaatggatt ttctaaaaaa tcataccaag
    28861 agcgcctcga gctgttaaaa gctcaggcgc tccttagtcc tgagagacaa gctagtctgg
    28921 agaaggatga acagatgagc gtgactgtgg cagaccagct gagtgagaat gtagtgggaa
    28981 ctttttctct gccttattca ctggttccag aggtacttgt caacggtcag gaatacaccg
    29041 ttccctacgt gacagaagaa ccctctgtgg ttgcggcggc cagctatgcc agcaaaatca
    29101 tcaagcgtgc aggtggtttt actgcacaag tccatcagcg acagatgatt gggcaggtag
    29161 ccctttatca agttgctaat cctaaactag cgcaagagaa gattgccagc aagaaagcgg
    29221 agctcttgga gcttgccaat caagcctatc cttctatcgt taaacgtgga ggtggggcgc
    29281 gtgatctgca tgtcgagcag ataaaaggcg aaccagactt tctcgttgtt tatattcatg
    29341 tcgataccca ggaagccatg ggtgccaata tgctcaacac catgctggaa gccttgaaac
    29401 cagtcttaga agaactcagt cagggacaga gtctcatggg aatcctgtcc aactacgcga
    29461 ctgattctct ggtgactgca agctgtcgca tcgcctttcg ctacttgagc cgccaaaagg
    29521 atcaaggacg agagattgcg gagaaaattg cgttggctag tcagtttgcg caggctgatc
    29581 cttaccgagc tgctactcat aataaaggaa tttttaatgg tattgatgcg attttgattg
    29641 ccactggtaa tgactggcgt gccatcgaag ctggggccca tgcctttgcc agtcgagatg
    29701 gacgctatca aggtcttagc tgctggacgc tggaccttga aagagaagaa ttggtcggtg
    29761 agatgaccct gcccatgcct gtagcgacta agggtggctc tatcggcctc aacccacgtg
    29821 tagctctcag tcatgatcta ctaggaaatc cttctgccag agaattagcc cagattatcg
    29881 tgtccatcgg tcttgctcaa aattttgcag ccctcaaagc cttggtaagt acgggcatcc
    29941 agcaaggcca catgaaacta caggccaaat ccctagctct cctagctggg gctagtgaat
    30001 ctgaagttgc tcccctagta gagcgcctca tctcagataa aacctttaac ctagagacag
    30061 cccagcgcta tctcgaaaat ttaagatcat aaaaactcag acgaatcggt ctgagttttc
    30121 ttgtgtttaa atactttttc ctggtaatag cgtaactggt aagaagtaac cagttgtggc
    30181 gacttccttg ccttcaatct tttgcaagag aagatcaata gtgagacaag caatctcttc
    30241 caaaggttgc ttgatagtag ccaattgagg gtagtaattt tcgataaagt aggtcccatc
    30301 atagccgatg accttgagct cttttgggac agaaatgccc aattcttgag cgattttaat
    30361 gaccagaata gctgtcaaat catccgaagc aaaaatggca tctggttttt cccgggtcaa
    30421 gatattcttg atttccattt cttttctgac gggagaaaag tcactggaaa cattgataat
    30481 aggagctttt gggagtacgg atgcaaaacc agcgtggcgc agtccggttg gcgaattgga
    30541 attgtcattc cctgtaatca tgatgataga ctgggcacct gtcttgacca aggtttgggc
    30601 agcaagaacc ccaccagcat agttgtcaga ggagacaaca gggatgtctg gcgataggtt
    30661 tcggtcaaag gaaataatcg gcgctgtcac acgattgtag tcttcgattc ctaggttgtg
    30721 actaccagaa atgatgccgt ctacctgatt ggcttccaac atttcgatgt attcgcgttc
    30781 cttctcagaa tcatgttcac tgttgcagat gatggtcttg taaccatttt tgaagagttg
    30841 gtgttccaat ttatcaatca attctgcata gaaaacattg gaaatattgg ggaaaatcaa
    30901 gccgattaac ttagctgatt ttccttgcag actacgagcc aggttgttgg gtttatagcc
    30961 caattctctc atggcttcat tgactttttg gatggttttc tcagatagat accctttttt
    31021 attgataacc cgagaaacgg tagtaggact gacgcctgca agtttggcga catcagttag
    31081 ttttgcgacc ataatctaat tcatagtaag ttccagttgg gtttccagat ttgatcagga
    31141 taccattttg gtccgcatgt gggaagacac gaccagaaaa tactttttct cctttattga
    31201 tgaaaatttc aaagacagag ttatcgatga agattgtagc agtagtagcc tgattctcga
    31261 tagggcaaga acgagttgtc ccaaattctt gggcatactg ttctccagcc tggctacgat
    31321 ccactgtcac ttgaccgttt acaaggtcaa agttgattga aagtccctta ccttctttat
    31381 cagcaagtaa gacaatctcg ctctggatat tagcttccaa gttgagttca agttcgtaag
    31441 tgttcttggt ttgggaacgg tttgagaagg cttcttcaga agcacgaagg tccttaatag
    31501 cagcgactgg atactggtag agcttgtcgt ctttgatagt gagttccttg accaaagaga
    31561 aggttccttg gtgatcaaaa cagtcagatg ggtaagaaac atctggcaaa ccaagccagc
    31621 taactgctag agcacgccca tcaggagcgt tgaaggcttg agttgcatag gcttcgaaac
    31681 cgtaatccat gttttgaagt tgagacacat ctaccatttt ggcattttta gggtcaaagg
    31741 aagccccaat cttatacata tttggaaaga tattatcgta gtctagaact ttcttatcca
    31801 atccttgtgg acagtagaga aggacaggct gttcctctac aaagaccaga ttaggacatt
    31861 ccatcatgta ggcagtacgg tcgttagcaa agtcaaggtc gccaactgct tgccagtttg
    31921 tgtagtcgtt attgacagcc ttgtagagac ggacgaagcc ttttttctcc aagttctgtc
    31981 caccgacgac agcatagtat tgacctttaa agttaaaaat ttgtggatcg cggaagtggt
    32041 cagtagagtc tgctggctgg tcaatcaaga tcttgtcaat ctttgtaatc ttaccctcct
    32101 tgtccatcaa agcaccgatt tggtatgggt gacggatcca gttttcatcg cgaacatttc
    32161 ctgtataaaa taggaataag ttatcgccaa attgcatggc agaaccagag taggcaccgt
    32221 ggctatctaa tggagtatcc ggcaaaactt tgactccagt ttctctaaag tgaatcaaat
    32281 catcactttc tagctgtgcc caagatttta aaccgtgggc tgcaccaaaa ggaaaattct
    32341 ggtaaaagag gatccacttg ccatcaaagt aagaaaagcc atttgggtcg ttgagaagtc
    32401 ctgtttttgg ctcaacatgg taatgagtat gccatggaga ttgtgccata ttttccttta
    32461 tatgttgaat ttcttcttgc gtccaatctt gataaagtct gtaacgacgc tcagttgtcc
    32521 attccattca ttcctccaaa aatcttatca cttttaatag taactgtttt cgataaaaaa
    32581 tgcaagccct ttatgttaaa aaagagaaaa aactgtcaaa cgattatcat aaaataaata
    32641 caggaaatca atcaaatttt tacgaaaatg tgaaagataa tgaaataaaa gactttttaa
    32701 ccaaggtttc aaacttattt ttcaagaaat tcctgatgaa acagtcaaaa acaatcaatc
    32761 taaagcttgc ccactaagta gatagaatat tttttctgca aaacgcttga catattttta
    32821 aaacatgata aaataatagt cgtaagggcg aataaaatcg ctttcaaaca agaacaaaat
    32881 gttatataag gagatttttg ctatggatta tagtaaagtt gctgccgagg tcattgaggc
    32941 tgtcggtaag gataatcttg tagctgcagc tcactgtgcg acacgtcttc gcttggtatt
    33001 gaaagacgaa gcaaaagtga atcaagcagc tctggataat aatgcggatg tcaaggggac
    33061 ttttagcact aacggacaat atcaaattat cattggtcct ggtgatgtta acttcgtata
    33121 tgcggaaatt attaagaaaa ctggtttgaa agaagtttcg acagatgatt tgaaagagat
    33181 tgctaataaa gacaaaaagt tcaatccatt gatggacttg attaaacttt tgtcagacat
    33241 ttttgttcct attatccctg ccttagttgc aggtggtctc ttgatggctt tacggaactt
    33301 cttgacatca ccagatttgt ttggacctca atcaattgaa gatatgtatc ctgctatcaa
    33361 aggcttctca gctatgattc aattgatgtc tgctgctcca tttatgttcc tccctgtttt
    33421 ggttggtatt tcagcagcca agcgctttgg agcaaaccaa ttccttggtg cggcaatcgg
    33481 tatgattatg accactccag atttgggtgg taaagaagcc ttctgggaca tcttaggttt
    33541 ccatgttaca caaacaaact atgcttatca ggttattcca gtccttgttg cagtctggtt
    33601 gttagctaat ttagaaaaat tcttccacaa gaaattgcca tctgcagttg acttcacttt
    33661 tacaccgctt ttatcagtga tgattacagg attccttacc tttacagtta tcggtcctgt
    33721 tatgttggtt gtgtctgatg cgattacaaa tgcgattgta tggttgtaca acacaacagg
    33781 tgcctttggt atggggctat ttggtggaac ttactcatta atcgttatga cgggtcttca
    33841 ccaatcattc ccagcaatcg aaacacaatt gttgtctgca tacaataata acggtactgg
    33901 atttggagac tacatctttg ttgttgcttc aatggcaaac gtagcacaag gtgcggctac
    33961 tcttgcagtc tacttcttga caaaaaatgc taaaacaaaa ggattgtcca gttcggctgc
    34021 tgtttctgcc ttccttggta ttacagaacc tgctctcttt ggggtaaact tgaaatacaa
    34081 attcccattc ttctgcgccc ttgctggttc agcaattggt gcctttgtag caggtttaat
    34141 tcacgttatt gcagtttcat tgggagcggc tggatttatt ggattccttt ctattaaggc
    34201 tggttcaatc cctatgtata taattgcgga aattatgtcc tttgttgccg cctttgcttt
    34261 cacttatttc tacgggaaaa caaaagcagc tagtgtcttt gctgatgaag ctgccacagc
    34321 aaccgcagaa acagttacag aacctacagt agaagcacca gtagtggaag agactgatac
    34381 tcttcaaaat gaaacacttg taactcctat cgttggtgat gttgtcgctc ttgctgatgt
    34441 caatgaccca gtcttctcaa gtggagctat gggacaaggt atcgctgtga aaccaagcca
    34501 aggcgtggtc tatgcaccag ctgatgctga agtttcaatt gcctttccaa cagggcacgc
    34561 ttttggtttg aaaacaagaa atggtgctga agttttgatt cacgttggta ttgacactgt
    34621 atctatgaac ggtgacggtt ttgaagcaaa agttgctcaa ggtaataagg tgaaagctgg
    34681 cgatgttctt ggaacatttg actcaaacaa aatcgctgca gctggacttg atgatacaac
    34741 aatggttatc gttacaaata cagctgacta cgcttcagta gctccagtcg caacaggttc
    34801 agttgcgaag gggaatgctg tgatcgaagt gaaaatctaa tcaatcctct ctaatgtgaa
    34861 aacgaacaaa tgacatgttt gttcgttttt attagatagt aagatttaca gaggaaaatc
    34921 taaaaaatag agacatttag actttcgaag tatgctataa taaagaaaat aaaaacaaga
    34981 ggtttatcat gacaaaatta tatggaagct tggaagcggg cggtacaaag tttgtctgtg
    35041 ctgtcggtga tgaaaacttt aacgttgtag aaaaaacaca atttccaaca acaactccaa
    35101 tcgaaacaat cgataaaacc attgagttct tctcaaaatt cgataacctt gctggtcttg
    35161 cagttggttc atttgggccg attgatattg acaaaaactc aaaaacttat ggctttatca
    35221 cgacgactcc aaaaccaaac tgggcaaatg tggacttgct tggtgccctt cgtcgcgccc
    35281 taaacgtgcc aatgtacttc acaacagacg taaacagctc tgcttacggt gaaatggttg
    35341 cccgtaacaa tgctggtggt cgtatcgaaa acttggttta ctacacaatc ggtacaggta
    35401 tcggtgcagg cgtcatccaa cgtggtgagt ttatcggtgg tgtgggtcac cctgaaatgg
    35461 gtcattatta tgttgctaga cacccaatgg atattgaaaa agagtttaag ggtgtttgtc
    35521 ctttccataa gggatgtcta gaaggttatg cagctggtcc aagtttggaa gctcgtacag
    35581 gtgtacgtgg ggaaactatt gaactcaaca accctgtttg ggatgttcaa gcctactata
    35641 tcgctcaagc tgcggttaat gcgacagtga ctttccgccc agacgtgatt gtctttggtg
    35701 gaggggtcat ggctcaacaa catatgctgg accgtgtccg tgagaaattt acatctcttc
    35761 ttaatggtta cctaccagta ccagatgtgc gtgactatat cgtgacgcca gcagtcgcag
    35821 gaaatggttc tgccacactt gggaactttg ttcttgcaaa agaagtttca aaataaagca
    35881 caaaatccgc ttgggaaacc aaacggattt tgtggttgaa aacaatattt ttgagccttt
    35941 ccttaagtga ttacggacgg tagcgacttt cttcgaaatt ccatacctaa actttgagcc
    36001 tagtgtctca aagtttccga atacctgaaa ccattagatt tcaggtgttt ctatcacggc
    36061 ggaaaggcta ggtaagtcct cgcctcacta tcgaaaaata tgcatgattt ccataccaaa
    36121 gtatatttag tatcaagcta tagttaatct acgctttaaa aatccacttg ggaaaccaaa
    36181 cggatttttt acgtgtcaaa gcatttgcca gaaaaagtca ataatatagg tcagaccgta
    36241 ggtataaacc acgagggtaa agggtttggt cagaatgatg atggtcatgt agcgcttgaa
    36301 actcatcttg gtcagggcag ccagcataca gagaaagtca gctgggctaa tgggccaaat
    36361 catcataaaa ataaagaagc ggtcaaaacg attgccctta tctagccagc cgatgtactt
    36421 gtcgtaggtg cgcttgctga cgacagactg gacaaaggca gctccgtata ggcgcactag
    36481 ataaaagata atggcacagc caatcacgat gccgatatag ttgtagatag tcccgatgat
    36541 gtgcccgtag ataaagaccc cagccaccga ggtcaaggcc cctggaatga tagggacgac
    36601 agtctgtaaa atctgtaaaa agataaagag aggtggaccc cagatgcctg cctgctggat
    36661 aaaggcagag agggtttcct tggattgtaa aatcccagcc tgataagccc aaatacagaa
    36721 aatcaaactc ccaaccccac cgacaatcga tgagatattg ataactcgct gcagaagggg
    36781 agaaacagct ttcatttgtt tatcctgtga catgtaaact cctcatagat agtatatttc
    36841 tactatacca tattttggag gagaaagggg gaatgagtct tttttgataa ggaatagaaa
    36901 gtaaatagcc cttcgagact tagaataggt ctgtcaacta aaggttatat gcattctagg
    36961 tttcgaataa tcgcaattcc ataaatttaa aagcattcta agtttcgaat agcttatctg
    37021 agataatttt gaggcattcg cgctttcgaa tagtcatttt ccctaaagtt tagatgtatt
    37081 ctgaagctag aaagactaaa atctaaaagt agtaagagtc tctctctgat ggacgtaatt
    37141 tatggctagt atgaatcctt tgaattgtta gttaatttta atattaatca gttctgaaat
    37201 aattactaaa tatagttaga tggtgaatta attattgcta aatttcaagt tcaagttagt
    37261 catccagaag tcttctctgg gggacttgta gttcaagcat tttttaggat agttgttaat
    37321 ccacttttcg atgaatgcga cttcctttgt agtcgttttc ttagttccct gaggtaacca
    37381 tctatgaatg aacttgttgt gattttgatt agatcctata gtggtgtgca taatagatat
    37441 agcagattga aactagaata gtatacatct acttctaaaa cattgttaga aatcaatttg
    37501 actgtcctga tcaatttgtc ctgttcttat ttcattttac tatataatcc ttagaaaaca
    37561 tatcggacaa gcggttgaat tccgttccgt ccattagtag ataaaggatt actccttctt
    37621 gaaatgacag atgaacttgt ttcttataaa aatgttatct gaaactattc gcttttttcc
    37681 agaagtttta gaggaagaaa actttatgag gaaaaaggag ttactcaaat aacaattcag
    37741 gattaaaaat agacagttga ggagcggaag gtataaatta agtttgctac tgtataatgg
    37801 atttatcact aatactcttc gaaaatctct tcaaaccacg tcagcttcgc cttgccgtag
    37861 atatagttac tgacttcgtc agttctatcc acaacctcaa aacagtgttt tgagctgact
    37921 tcgtcagttt tatctgcaac ctcaaagctg tactttgagc agcctacggc tagctttcta
    37981 gtttgctctt tgcttttcat tgagtataaa agtgctccag gccatatttt acacagcaat
    38041 agtcttctaa aaatggacaa aaaacattgt aaaggctatc aaaaaggagt ataatgagtg
    38101 caagaaattt cggaggtgaa agatgctaga agtaagaagt ctagagaaaa gttttggctc
    38161 caagcaagtt ttgtttggta ttgactttca agcgcgacca ggtcgtattt tgggactagt
    38221 cggaaaaaat ggtgctggaa aaacaacgat tttccatagt attttgaaat tcctagaata
    38281 tcagggagaa atcggtctag atggtcagga tattcgtcag gagacctatg ctcggattgg
    38341 ttatctgcct gaagaacgca gtctcatgcc taaattgaca gttcttgaac aagttcgcta
    38401 cttggcgact ctaaaaggca tggatgccaa agaagtcaaa gaaaaactcc ctcaatggat
    38461 gaagaggttg gaagtgaaag gaaagctgac agataaaatc aagagtctgt caaaaggaaa
    38521 tcagcagaag attcagctca ttattactct gattcatgaa ccagacctga ttatcttgga
    38581 tgagcctttt agtggattgg acccagttaa tacagaattg ctcaaacaag tcatttttca
    38641 ggaaaaagag cgcggagcaa ccattatctt ttctgaccat gtcatgacca atgttgagga
    38701 actttgtgac gatattctga tgatccgaga tggccgtgtg gtcttgcacg gaccagttca
    38761 ggatgtccgc aatcaatacg ggaaaacgcg tctctttgtt tcaagtgaac gaagcaagga
    38821 agaattggaa aatcttcctc atgtcaaaca ggtgagcttg accaaacaag gcagttggaa
    38881 attgattttg gaggatgaga gcgctggaag ggaactcttc ccaatcttga ctcaaggtca
    38941 atatatcgca acatttgacc aacaagcgcc aactatcgat gaaatcttta aactagaatc
    39001 aggagtggaa gtatgagaaa tatgtgggtt gtaatcaagg aaacctatct tcgacatgtc
    39061 gagtcatgga gtttcttctt tatggtgatt tcgccgttcc tctttttagg aatctctgta
    39121 ggaattgggc atctccaagg ttcttctatg gctaaaaata ataaagtgga agtagtgaca
    39181 acagtgccat ctgtagcaga aggactgaag aatgtaaatg gtgttaactt cgactataaa
    39241 gacgaagcaa gtgccaaaga agcaattaaa gaagaaaaat taaaaggtta tttgaccatt
    39301 gatcaagaag atagtgttct aaaggcagtt tatcatggcg aaacatcgct tgaaaatgga
    39361 attaaatttg aggttacagg tacactcaat gaactgcaaa atcagcttaa tcgttcaact
    39421 gcttccttgt ctcaagagca ggaaaaacgc ttagcgcaga caattcaatt cacagaaaag
    39481 attgatgaag ccaaggaaaa taaaaagttt attcaaacaa ttgcagcagg tgccttagga
    39541 ttctttcttt atatgattct gattacctat gcgggtgtaa cagctcagga agttgccagt
    39601 gaaaaaggca ccaaaattat ggaagtcgtt ttttctagca taagggcaag tcactatttc
    39661 tatgcgcgga tgatggctct gtttctagtg attttaacgc atattgggat ctatgttgta
    39721 ggtggtctgg ctgccgtttt gctctttaaa gatttgccat tcttggctca gtctggtatt
    39781 ttggatcact tgggagatgc tatctcactg aataccctgc tctttatttt gatcagtctt
    39841 ttcatgtatg tagtcttggc agccttccta ggatctatgg tttctcgtcc tgaggactca
    39901 gggaaagcct tgtcgccttt gatgattttg attatgggtg gtttttttgg agtgacagct
    39961 ctaggtgcag ctggtgacaa tctcctcttg aagattggtt cttatattcc ctttatttcg
    40021 accttcttta tgccgtttcg aacgattaat ggctatgcgg ggggagcgga agcatggatt
    40081 tcacttgcta ttacagtgat ttttgcggtg gtagcaacag gatttatcgg acgcatgtat
    40141 gctagtctcg ttcttcaaac ggatgattta gggatttgga aaacctttaa acgtgcctta
    40201 tcttataaat agaagagcct tgcgaaagcg aggtttttaa aggtgaaaag ggtggaatca
    40261 cgaacctaat tatgtacaca ttatgtaaac aagttgcttg aaaactcaat tgtatagtga
    40321 actgaatctg gaataggata atattacttc taaaacattc ttacaaattg atttgaattc
    40381 caaaatccca tcgttccgtt cttatttcat tttgcgatat agtgaaagga aataagtcaa
    40441 ctcacaaaaa acgcataata tcaaggattt ttaacacttg atactatgcg ttttctaatt
    40501 ttaaagactt tttgcctggt ctcttttgtt ctatgagtat gttacatatc taggtaaaaa
    40561 ttatttaaag caaattaacc ttatttctca agatgtagta ggttccgaga taaaagatag
    40621 ctatgaatat gagttcttta acaatggcta tacctgctcc catatagaaa tggtcattga
    40681 gtcctaccag tgaattgaca tagaaattag aactaagatt gaagaaaagt tcaataaatc
    40741 caatgacgat ttggatacca atgtaggctg caacagcgag tgctgtacgg tattcattga
    40801 aaagctgtcc aatggaaata gccaggtaga tgcagaggat tcctgaaatg gtatttagta
    40861 ggaaggatat acctgtaaga aagatctgag ggagatgtgt ttctacaaat gtaatcacat
    40921 aagaaagagg aatccattct ggagctgtta aagccagaat aataacagca cttagagcca
    40981 atacagcggt gctaatcaat gaccagataa aggcaccgat tagtttggct gtgatgatat
    41041 ggtgttcaga aactggcaag gtcaaagtca gatagccttg tcggtcgtag acactacctt
    41101 tgaagcgttt aataatcaag aaaatggttg aaatcgcaag tgtaagcatc aagccaccaa
    41161 agactgtagc tagaaaaagt agcatagtag cctgactttc ttggtaaggt agatttttaa
    41221 agccttgtgt ctgtattccg atgagggcag aaaggactag cacggctgcg tagagggcta
    41281 aataccactt gttaacattt ttaaattcgt agtgaactaa attccaaaac ataaataatc
    41341 tcctttgctt aggccttaaa ttcctgacgg aagagttggt caatggattc acctgactcg
    41401 tagcgaatat catctacatt tccttgacgg acgacttttc cgtcttttag gaagacaatt
    41461 tcatccaaga ttggctcgat atcagaaatc aagtgggtag aaatcaaaac ggtagaagtt
    41521 ggtgagtagt tgttgataat ggtattgagg atataagcac gggctgctgg atccacccca
    41581 ccaatgggtt catccaaaac atagagacga gcatcacggc tcataaccaa aatcagttga
    41641 accttttctt tgtttccttt tgatagtttc ttgagacgac tattttcatc aatgcccagg
    41701 tctgcaagta gatgatgagc gcgttcaaga ttgaaatctt tatagaaggt cttgaagtag
    41761 gttagggctt ctttgacctt catttgctca ttgagatagg tcgtatcagg caaataagct
    41821 acaacggcct tggttgctgg gcttgggtcc atgtcgttga tgaggacacg tccttgatct
    41881 ggttgtaaga ggccattaat tagtttaatc agggttgttt ttcctgagcc gtttggccca
    41941 agaaggccga caatttttcc agctggaatg tcaagagaaa cattttcaag ggctggtgtt
    42001 gctccatatg atttggatac attttcaaat gctagtaatg acataggctt aaactccttt
    42061 aatataatca ctgactacgc ctggtagttc ttctttttca tagccaaaat gggtcatgga
    42121 ggaaacgaag tgttccaatt cttcttctga taattgtttg cgtgactggg cgattagctc
    42181 cttatcctta gtcacaaatc gtccagttgt tcgcttgctg tagacaaatc cttctcgttc
    42241 aaggtctgat aaggctcttt ggatggtatt gggattgaca ccagcctcgc tagctagctc
    42301 cctcacggtt ggaagttgtt gattgggttc cagtgtatgg gaaacaatct gaagcttgat
    42361 tttctccata atctgtaaat agatgggttt tttgttgtca aatgtccagg acatcttagt
    42421 ctcctttctt ttaccttgtt gtcttaatta aataatacaa caaaacaaaa aacttgtcaa
    42481 gcaaaaagaa gaattgtttt gggaatcgct taaaaaatgg tataatgaaa agaaaacgat
    42541 aaggagggaa aggatgcgtt gtccaaaatg tggggctacc aagtcaagtg ttatcgatag
    42601 tcgccaagca gaagaaggga acaccattcg tagaagacgt gagtgcgacg aatgccaaca
    42661 ccgttttaca acctacgaac gagtagaaga aagaacctta gtggttgtta aaaaagatgg
    42721 cacacgggaa caattctcca gagataaaat ctttaatggg attatccgct cagcccagaa
    42781 acgtcctgtg tcaagtgatg aaatcaacat ggtagtcaat cgtatcgaac agaaactccg
    42841 tggtcgaaat gaaaatgaaa ttcaaagtga ggacattggt tcactcgtca tggaggagtt
    42901 ggctgaattg gacgagatta cctatgtacg ttttgctagt gtctatcgta gttttaagga
    42961 tgtcagtgag ttagagagct tgctccaaca aatcacccag tcctctaaaa agaaaaagga
    43021 aagataaatg aagccaattg accgtttttc ttatctaaag aataatcggg tgtcgcaaga
    43081 tacctcatct ctggtacagt gctacctccc gattatcggt caggaggcac tgagccttta
    43141 tctttataca atcagttttt gggataatgg tagaaaggaa tatctttttt caagcattct
    43201 caaccatctc aactttggga tggatagact gataaaatca ttgaaaatct tatctgcttt
    43261 taatctcttg accctctatc aaaagggtga tgtttatcag ctagccctcc atgctcctct
    43321 atctagtcaa gacttcttgg ggcatcctgt ttatcgcaga ctcttagaga aaaagattgg
    43381 cgatgtagct gtggaggatt tgaaagttga aagtgctgat ggtgaagaaa tacctgtctc
    43441 actcaatcaa gtctttccag aattggcaga actaggcagt caagaagacc ttggtctcaa
    43501 gaagaaagtg gccaatgatt ttgacttgga acattttcgc cagctgatgg ctcgagatgg
    43561 acttcgtttt gcggatgagc agtccgatgt cttaaacctc tttgctattg ccgaggagaa
    43621 gaaatggact tggtttgaaa cctatcaatt ggccaagtca acagctgttt cccaggttat
    43681 ttcaaccaaa cgcatgcgtg aaaaaattgc tcaaaaacca gtttcctctg actttagtct
    43741 taaggaagcg accattatca aagaagctaa aagtaaaact gccctgcagt tcttagcaga
    43801 aatcaagcaa acacgcaagg gaaccattac ccaaacagaa agagaactct tgcaacagat
    43861 ggctggcttg ggcttactgg acgaagtcat caatattatt ctcttattga cctttaataa
    43921 ggtggattcg gcaaatatca atgagaaata tgccatgaag gtagccaatg actatgccta
    43981 tcaaaagatt cattcggcag aagaggccgt tttacgaatt cgtgatcgag ggcagaaagc
    44041 aaaaacacaa aaacagaatc agactgcccc agaaaaaacc aatgttccta aatggagtaa
    44101 tcctgaatat aagaatgaaa ccagcgagga aactcgtctg gaactagaac gtaagaagca
    44161 agaactatta gctcgattag aaaaaggagg agattagatg gaaagtgtcg gagacgtact
    44221 caaacgtcaa cccagccgtt tttattatca agatttggtc cagaaaatca tgaaggaccc
    44281 tgatgttgcg gcctttatcc agcaagaatc cttaactcca gaggaattaa atcgcagtat
    44341 ctccaagttt aatcagtaca tcaccgagcg tgacaagttt ctccgtgggg atacggatta
    44401 tattgccaaa ggctacaagc cgattttggt taagaatcat ggctatgcag atgtttcata
    44461 tgaagaaact cctgagctaa tcgcggctga aaaagaagcg gctattaaga accgtctcaa
    44521 gttaatcaat ctaccagcca gtctcaagaa agctagtttg gctcaagttg acttggatga
    44581 tttggggcgc ttaccagttt ttgaaaagct actagccttc gtggagcaat atccagctat
    44641 tcgaaaaggt ctttacttat atggagactt tggtgtgggt aaaagtttca tggtggcggc
    44701 cttagctcat gatttatcag aaaaacgtgg tgtttcatca actctcctcc actatcctag
    44761 ctttgtcatt gatgtcaaaa atgctatcag tgatggcaat gttaagacct tggtggatga
    44821 gattaagctg tctgaggtcc tgattttaga tgatattggc gccgagcaat caaccgtttg
    44881 ggtgcgtgac gaaatcctgc aggtcattct ccaatatcgg atgcaggaaa atttaccgac
    44941 ctttttcaca tccaacttca actttgaaga tttggagaag cattttgcga aagtgaaaca
    45001 tggaaatgac gaaacctggg aagccagacg ggtcatggaa cgcatccgtt atttggctga
    45061 ggagactcgt ttagaaggag taaaccgtcg atgacagaaa ccattaaatt gatgaaggct
    45121 catacttcag tgcgcaggtt taaagagcaa gaaattcccc aagtagactt aaatgagatt
    45181 ttgacagcag cccagatggc atcatcttgg aagaatttcc aatcctactc tgtgattgtg
    45241 gtacgaagtc aagagaagaa agatgccttg tatgaattgg tacctcaaga agccattcgc
    45301 cagtctgctg ttttccttct ctttgtcgga gatttgaacc gagcagaaaa gggagcccga
    45361 cttcataccg acaccttcca accccaaggt gtggaaggtc tcttgattag ttcggtcgat
    45421 gcagctcttg ctggacaaaa cgccttgttg gcagctgaaa gcttgggcta tggtggtgtg
    45481 attatcggtt tggttcgata caagtctgaa gaagtggcag agctctttaa cctgcctgac
    45541 tacacctatt ctgtctttgg gatggcactg ggtgtgccaa atcaacatca tgatatgaaa
    45601 ccgagactgc cactagagaa tgttgtcttt gaggaagaat accaagaaca gtcaactgag
    45661 gcaatccaag cttatgaccg tgttcaggct gactatgctg gggcgcgtgc gaccacaagc
    45721 tggagtcagc gcctagcaga acagtttggt caagctgaac caagctcaac tagaaaaaat
    45781 cttgaacaga agaaattatt gtagaaagtg agaaattatg gccctaccaa ctattgccat
    45841 tgtaggacgt cccaatgttg ggaaatcaac cctatttaat cggatcgctg gtgagcgaat
    45901 ctccattgta gaagatgtcg aaggagtgac acgtgaccgt atttatgcaa cgggtgagtg
    45961 gctcaatcgt tcttttagca tgattgatac aggaggaatt gatgatgtcg atgctccttt
    46021 catggaacaa atcaagcacc aggcagaaat tgccatggaa gaagcagatg ttatcgtctt
    46081 tgtcgtgtcc ggtaaggaag gaattaccga tgcagacgaa tacgtagccc gtaagcttta
    46141 taagacccac aaaccagtta tcctcgcagt taacaaggtg gacaaccctg agatgcgaaa
    46201 tgatatctat gatttctatg ccctcggttt gggtgaacca ctgcctatct catctgtcca
    46261 tggtatcggt acaggggatg tactagatgc gattgtggaa aaccttccaa atgaatatga
    46321 agaagaaaat ccagatgtca ttaagtttag cctgattggt cgtccaaacg ttggaaaatc
    46381 aagcttgatc aatgctatct tgggcgaaga ccgtgttatt gctagtcctg ttgctggaac
    46441 aactcgtgat gccattgata cccactttac agatacagat ggtcaagagt ttaccatgat
    46501 tgatacggct ggtatgcgta agtctggtaa ggtttatgaa aatactgaga aatactctgt
    46561 tatgcgtgcc atgcgtgcta ttgaccgttc agatgtggtc ttgatggtca tcaatgcgga
    46621 agaaggcatt cgtgagtacg acaagcgtat cgcaggattt gcccatgaag ctggtaaagg
    46681 gatgattatc gtggtcaaca agtgggatac gcttgaaaaa gataaccaca ctatgaaaaa
    46741 ctgggaagaa gatatccgtg agcagttcca atacctgcct tacgcaccga ttatctttgt
    46801 atcagcttta accaagcaac gtctccacaa acttcctgag atgattaagc aaatcagcga
    46861 aagtcaaaat acacgtattc catcagctgt cttgaacgat gtcatcatgg atgccattgc
    46921 catcaaccca acaccgacag acaaaggaaa acgtctcaag attttctatg cgacccaagt
    46981 ggcaaccaaa ccaccaacct ttgtcatctt tgtcaatgaa gaagaactca tgcacttttc
    47041 ttacctgcgt ttcttggaaa atcaaatccg caaggccttt gtttttgagg gaacaccgat
    47101 tcatctcatc gcaagaaaac gcaaataaaa aagtagaatc tggaatgaca gttccggatt
    47161 tttttgatat aataaaataa tagaaaacgc tatcaaaaga aggagggtga tgtcggtgat
    47221 ggaacatttg tttaaattct tacttttagc accgtacttt tattttgata actggattga
    47281 aaaggccaac agaaatagta agttttttcc aattttttat tatttttact ggttttacat
    47341 ccccttctat tctcttttta gccttgcttg gacagttgtt tcagttctgt ttttcaatac
    47401 cgtcttgaga aatgtgacag atatcaagtt atggggcatt tggtttcttt ttattctgct
    47461 agctattggt atgaattggt taacttattc ctgtttcaaa gaaatgtttc gcttgagaca
    47521 ggaactaggg aaatctaagg gtggaaggca ttgatttata tcggtttcta tttgtaggag
    47581 gtggcttatg gtacctctaa aatcatttat tacacgctat tccaaggttt atattgataa
    47641 taatatttaa tagaacgaaa ctaaagaaaa caatttaaaa aaggaggctt gtgatggaac
    47701 acttgtttaa aatgataatc ttattaccct gtttttactt tttcagctgg attgacaagg
    47761 ataatagaga aagtaaattt ttcccaattt tttactattt ttactggatt tacatcaccc
    47821 tctattctct ttttagcctt gcttggacag ttttttcagt tctgtttttc aatatcgtct
    47881 tgagaaattt gacagatatc aagttatggg gtatttggct attattacta cttatcgctt
    47941 ttgctagtga ctggttagac tatatctgtt tcaaaaaaat gcttgacttg agacgggaac
    48001 tagggaagtc taaaggcgta aggcattgat ttatactctt cgaaaatctc ttcaaaccac
    48061 gtcaacgtcg ccttggatta tatatgtaac tgacttcgtc agtcttatct acaacctcaa
    48121 agcagtgctt tgagcaactt gcggctagtt tcctagtttg ctctttgatt ttcattgagt
    48181 attatattac tttctatttg taggaggtgg cttatgaaga ttcctctctt aactttggca
    48241 aggcataaat ttgtttatgt cttgcttact ttgctttttc ttgctttggt ttatcgtgat
    48301 gttttgatga cttatttctt ttttgatatt catgcgcccg atctagctaa attcgatgga
    48361 caagcaatta aaaatgactt attaaaatca gcattagatt ttcgtattct ccagttcaat
    48421 ctaggttttt atcaatcatt tattattcca atcatcattg ttttgctagg ttttcaatat
    48481 attgagctga aaaataaagt tttacgattg agtattggaa gagaagtgag ttatcaaggg
    48541 ttaaaaagaa agttgacttt gcaagttgca agtatccctt gtttgatata tttagtgact
    48601 gtgctgataa ttgcaattat aacctatttc tttgggactt tttctcctct tggatggaat
    48661 tctctatttt ctgatggaag tggtttacaa agactcctag atggagagat aaaaagctat
    48721 ttgttcttta cttgtgtcct actaatcggt attttcatca atgcaatcta ttttttacaa
    48781 atagttgatt atgtggggaa tgtgactcgt tcggcaatca cctatttgat gtttctttgg
    48841 cttggttcta tgctgctcta tagtgccttg ccttactata tggttcctat gacgagtttg
    48901 atgcaagcta gctatgggga tgtaagtttg atgaaactct ttactcctta tatcctttat
    48961 attgtccctt atatggtgct tgaaaaatat gaagataatg tttaagaatt ttaacaatat
    49021 ttggctaaag agaaagatcg ttttactact tcgtatagtt ctgatgatga ttttgataaa
    49081 ctatctattg tcaacagcgg ttcaaaagca ggatgctgtt atctttttca agagagaatt
    49141 gatttcaatc ttttcctata atgactattc tgaagcgaat ttagaaatcc ccaaactctt
    49201 gttaaacctt tcgcttttca tggtaggatg gctctctgtc attttacttg aaagtgattt
    49261 ggcagaccat taccatcact tgattcgcta tcaatcaagc tcctttttcg attatacaag
    49321 gaaacgattg gttgtcattt ctaaattttt tactcaagat ttacttgtct ggttccttgg
    49381 tttacttccg ctaggaattc atttcaaaac agtcgcactt ttctttttac ttgctcagtt
    49441 aatgatgttg tacttactac tgtcttatct gatagcactg attagtgcgg gcgctggttt
    49501 ttcctttttt ct
//
LOCUS       DAVKJJ010000009        45594 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000009 DAVKJJ010000000
VERSION     DAVKJJ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45594
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.009"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            556..1008
                     /locus_tag="VW144_000511"
     CDS             556..1008
                     /locus_tag="VW144_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9599998.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1027..2364
                     /locus_tag="VW144_000512"
     CDS             1027..2364
                     /locus_tag="VW144_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU9599999.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2443..3327
                     /locus_tag="VW144_000513"
     CDS             2443..3327
                     /locus_tag="VW144_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9600000.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3327..4160
                     /locus_tag="VW144_000514"
     CDS             3327..4160
                     /locus_tag="VW144_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9600001.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4178..6271
                     /gene="nanB"
                     /locus_tag="VW144_000515"
     CDS             4178..6271
                     /gene="nanB"
                     /locus_tag="VW144_000515"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEU9600002.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPALIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISPLLLDKLSGDSQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSSSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6283..7386
                     /locus_tag="VW144_000516"
     CDS             6283..7386
                     /locus_tag="VW144_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9600003.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7560..8258
                     /locus_tag="VW144_000517"
     CDS             7560..8258
                     /locus_tag="VW144_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU9600004.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8277..9794
                     /locus_tag="VW144_000518"
     CDS             8277..9794
                     /locus_tag="VW144_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9600005.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNIADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9919..11247
                     /locus_tag="VW144_000519"
     CDS             9919..11247
                     /locus_tag="VW144_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9600006.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11499..12365
                     /locus_tag="VW144_000520"
     CDS             11499..12365
                     /locus_tag="VW144_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9600007.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12381..13220
                     /locus_tag="VW144_000521"
     CDS             12381..13220
                     /locus_tag="VW144_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9600008.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13493..13945
                     /locus_tag="VW144_000522"
     CDS             13493..13945
                     /locus_tag="VW144_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9600009.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            14042..14386
                     /locus_tag="VW144_000523"
     CDS             14042..14386
                     /locus_tag="VW144_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600010.1"
                     /translation="MVSSKYAARASFLDGQGITVDEMSWIIRGIVNGLIGRYIKLGTY
                     AAKYGISMARSILSRAAATAAARVRLLTKIFGWILRIAVLAADVYGNFANNIAAAWDA
                     HDKIPNNGRINF"
     gene            14673..15317
                     /locus_tag="VW144_000524"
     CDS             14673..15317
                     /locus_tag="VW144_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9600011.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLTWKEVLQKGLILASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15486..16403
                     /locus_tag="VW144_000525"
     CDS             15486..16403
                     /locus_tag="VW144_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU9600012.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKDKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            16421..17305
                     /locus_tag="VW144_000526"
     CDS             16421..17305
                     /locus_tag="VW144_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9600013.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17321..18172)
                     /locus_tag="VW144_000527"
     CDS             complement(17321..18172)
                     /locus_tag="VW144_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU9600014.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDGNCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18372..20429
                     /gene="pbp2b"
                     /locus_tag="VW144_000528"
     CDS             18372..20429
                     /gene="pbp2b"
                     /locus_tag="VW144_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEU9600015.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEINKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20440..21036
                     /gene="recR"
                     /locus_tag="VW144_000529"
     CDS             20440..21036
                     /gene="recR"
                     /locus_tag="VW144_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEU9600016.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21212..22255
                     /locus_tag="VW144_000530"
     CDS             21212..22255
                     /locus_tag="VW144_000530"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU9600017.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            22339..23712
                     /locus_tag="VW144_000531"
     CDS             22339..23712
                     /locus_tag="VW144_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU9600018.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAYLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFNANFLEQVLDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIHQAFQDLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGNQSVQLHNQMILSLSPDVLDTVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLSANDQILLKGSNSMNLAMLVE
                     SLENETK"
     gene            23699..24310
                     /locus_tag="VW144_000532"
     CDS             23699..24310
                     /locus_tag="VW144_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9600019.1"
                     /translation="MKPSDFVKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQGSDLDAEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEGSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDINRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDQLPVLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            24401..25105
                     /locus_tag="VW144_000533"
     CDS             24401..25105
                     /locus_tag="VW144_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEU9600020.1"
                     /translation="MNLWDIFFTTQATKPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIVKEA"
     gene            25324..26697
                     /gene="ftsA"
                     /locus_tag="VW144_000534"
     CDS             25324..26697
                     /gene="ftsA"
                     /locus_tag="VW144_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU9600021.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26714..27973
                     /gene="ftsZ"
                     /locus_tag="VW144_000535"
     CDS             26714..27973
                     /gene="ftsZ"
                     /locus_tag="VW144_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU9600022.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETAELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            27978..28649
                     /locus_tag="VW144_000536"
     CDS             27978..28649
                     /locus_tag="VW144_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU9600023.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28659..29198
                     /locus_tag="VW144_000537"
     CDS             28659..29198
                     /locus_tag="VW144_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEU9600024.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTSVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29198..29461
                     /locus_tag="VW144_000538"
     CDS             29198..29461
                     /locus_tag="VW144_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU9600025.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29458..30243
                     /locus_tag="VW144_000539"
     CDS             29458..30243
                     /locus_tag="VW144_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU9600026.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30252..31040
                     /gene="divIVA"
                     /locus_tag="VW144_000540"
     CDS             30252..31040
                     /gene="divIVA"
                     /locus_tag="VW144_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEU9600027.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMAELQARIEV
                     ADKELSEFEAQIKQEVEAPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31388..34180
                     /locus_tag="VW144_000541"
     CDS             31388..34180
                     /locus_tag="VW144_000541"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU9600028.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEYGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEGPAPEAALSFEDVAFTVERATGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
     gene            34583..34968
                     /locus_tag="VW144_000542"
                     /pseudo
     CDS             34583..34968
                     /locus_tag="VW144_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            35503..36195
                     /locus_tag="VW144_000543"
     CDS             35503..36195
                     /locus_tag="VW144_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEU9600029.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            36248..38659
                     /locus_tag="VW144_000544"
     CDS             36248..38659
                     /locus_tag="VW144_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU9600030.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGRI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALVTTSYVYKGTRFE
                     REAFASFPDDLLVQRFTKEGAETLDFTIELSLTCDLASDEKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLEAVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            38909..39610
                     /locus_tag="VW144_000545"
     CDS             38909..39610
                     /locus_tag="VW144_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9600031.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            39612..42320
                     /locus_tag="VW144_000546"
     CDS             39612..42320
                     /locus_tag="VW144_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9600032.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALTTHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEELEQILSDNGKVRLRLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQAQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLAFVLSLLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKAL
                     YKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVVAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLIAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(42416..42907)
                     /gene="tpx"
                     /locus_tag="VW144_000547"
     CDS             complement(42416..42907)
                     /gene="tpx"
                     /locus_tag="VW144_000547"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEU9600033.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(43030..43959)
                     /gene="psaA"
                     /locus_tag="VW144_000548"
     CDS             complement(43030..43959)
                     /gene="psaA"
                     /locus_tag="VW144_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEU9600034.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEANLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(43991..44839)
                     /locus_tag="VW144_000549"
     CDS             complement(43991..44839)
                     /locus_tag="VW144_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9600035.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(44836..45558)
                     /locus_tag="VW144_000550"
     CDS             complement(44836..45558)
                     /locus_tag="VW144_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9600036.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT        13879 a         8138 c        10009 g        13568 t
ORIGIN      
        1 aaactaattt agagaatgaa atatagagta tttctctctt aaaagttttt ggtgaaacga
       61 gatgtagaaa ggagatttag ccaaagagtc tattagtgct agaataatag attagaatta
      121 ttttagaaaa acgaagtgag cagcttataa attcaagtcc ccaaatagat tcatactagt
      181 atcttttgca aaaaataaag ggcgacttcc ttcatgaata tcaatttcat ctataaggaa
      241 ggtagctaat tgaactaact tatttattct gtttgtcgct agaaaaatca gacctccttg
      301 tgaagattga ggagatactt aatgaaaatc aaagaagaaa ctagcaagct agtagcagat
      361 tgcccaaaac accgctttga ggttgtagat aagactgacc tatataatcc aaggtgaagc
      421 gactgtggtt tgaagagatt ttcaaagagt ataggctaga gagtagtgtt tttatgtcct
      481 tctagtagaa aatgctagac agaagaatgg ggaacttgga taggaaaaat agattgagaa
      541 aggaggttag aagagatgat tattacaaaa attagccgtt taggaactta tgtgggagta
      601 aatccacatt ttgcaacatt aatagatttt ctagaaaaaa caggactaga aaatttaaca
      661 gaaggttcga ttgctatcga tggtaatcga ttgtttggga attgctttac ttatctagca
      721 gatggtcaag caggggcttt ctttgaaacc caccaaaaat atttggatat tcatttagtt
      781 ttggaaaacg aagaagccat ggctgttaca tcgccggaaa atgtaagcgt tacccaagaa
      841 tatgatgaag agaaagatat tgaattatac acagggaaag tggaacagtt ggttcatttg
      901 agagctggcg aatgcctcat cacttttcca gaagatttac atcaacccaa ggttcgtata
      961 aatgatgaac ctgtgaaaaa agttgtcttt aaagttgcga tttcttaatg tagaaagaga
     1021 agaacgatga aaaaaatgag aaagttttta tgtctagctg gaattgcgct agcggctgtt
     1081 gccttggtag cttgttcagg aaaaaaagaa gctacaacta gtactgaacc accaacagaa
     1141 ttatctggtg agattacaat gtggcactcc tttactcaag gaccccgttt agaaagtatt
     1201 caaaaatcag cagatgcttt catgcaaaag catccaaaaa cgaaaatcaa gattgaaaca
     1261 ttttcttgga atgacttcta tactaaatgg actacaggtt tagcaaatgg aaatgtgcca
     1321 gatatcagta cagctcttcc taaccaagta atggaaatgg tcaactcaga tgctttggtt
     1381 ccgctaaatg attctatcaa gcgtattgga caagataaat ttaacgaaac tgccttaaat
     1441 gaagcaaaaa tcggagatga ttactactct gttcctcttt attcacatgc acaagtcatg
     1501 tgggttagaa cagatttgtt aaaagaacat aatattgagg ttcctaaaac ttgggatcaa
     1561 ctctatgaag cttctaaaaa attgaaagaa gctggagttt atggcttgtc tgttccgttt
     1621 ggaacaaatg acttaatggc aacacgtttc ttgaacttct acgtacgtag tggtggagga
     1681 agcctcttaa caaaagatct taaagcagac ttgacaagcc aacttgctca agatggtatt
     1741 aaatactggg ttaaattgta taaagaaatc tcacctcaag attctttgaa ctttaatgtc
     1801 cttcaacaag ctaccttgtt ctatcaagga aaaacagcat ttgactttaa ctctggcttc
     1861 catatcggag gaattaatgc caacagtcct caattgattg attcgattga tgcttatcct
     1921 attccaaaaa tcaaagagtc tgataaagac caaggaattg aaacctcaaa cattccaatg
     1981 gttgtttgga aaaattcaaa acatccagaa gttgctaaag cattcttaga agcactttat
     2041 aatgaagaag actacgttaa attccttgat tcaactccag taggtatgtt gccaactatt
     2101 aaggggatta gcgattctgc agcctataaa gaaaatgaaa ctcgtaagaa atttaaacat
     2161 gctgaagaag taattactga agctgttaaa aaaggtactg ctattggtta tgaaaatggg
     2221 ccaagtgtac aagctggtat gttgactaac caacacatta ttgaacaaat gttccaagat
     2281 atcattacaa atggaacaga tcctatgaaa gcagcaaaag aagcagaaaa acaattaaat
     2341 gatttatttg aggctgttca gtagatgtaa aagactagaa aataggtggg atagtgagct
     2401 gaaaagctct agcccaatct tgtaaaagaa gggagaagga gaatggttaa agaacgtaat
     2461 ttaactcgct ggatatttgt tttgccagct atgattatcg taggattact ctttgtttat
     2521 ccgtttttct cgagtatttt ttatagcttt accaataagc atttgattat gcctaattat
     2581 aaatttgttg gtttggctaa ctataaagct gtgctatcag atcccaactt ctttaatgcg
     2641 ttctttaatt caattaagtg gaccgttttc tcattagttg gtcaagtttt agtagggttt
     2701 gtattggctt tagctcttca cagagtacgc cacttcaaga aattatatag gacattattg
     2761 attgttcctt gggcatttcc taccatcgtt attgccttct cttggcagtg gattctaaac
     2821 ggggtttatg gctacttacc taatctaatc gtaaaattag gtttaatgga acatacacct
     2881 gcatttttga cagatagtac atgggcattc ctatgtttgg tgtttatcaa catttggttt
     2941 ggagcaccaa tgattatggt taatgtgctt tcagctttgc aaacagtacc agaagaacaa
     3001 tttgaggctg ctaagataga tggtgcttca agttggcagg tgttcaagtt tatcgtcttt
     3061 ccacatatta aagtggttgt aggacttcta gttgttttga gaactgtatg gatctttaat
     3121 aactttgaca ttatctacct cattactggt ggtggaccag ccaatgctac aacgacgctt
     3181 ccaatttttg cttacaacct gggctgggga actaaattgt tgggtcgtgc ttcagcagtt
     3241 acagtattgc tctttatctt cttggtggcg atttgcttta tctactttgc tatcatcagt
     3301 aagtgggaaa aggagggtag aaaataatga agaagaaatc cagtatttat ttagatattc
     3361 tctcacatgt acttttagtt ggtgcgacca tcgttgcaat tttcccattg gtatggatta
     3421 tcatatcttc tgtcaaaggg aaaggggaat taactcagta tccaacacga ttttggcctg
     3481 aacagtttac attagattat ttcactcatg ttatcaacga tttgcacttc attgataaca
     3541 ttcgaaacag tttaatcatt gccttggcta caacccttat tgcgattatt atttctgcta
     3601 tggcagccta tggtattgtt cgattctttc ctaaattggg agcaatcatg tcgagactac
     3661 tcgtcattac ctacattttc ccaccaattt tgttagcaat tccctattca attgccattg
     3721 ctaaagttgg gttaacaaat agtttatttg gcttgatgat ggtttatcta tcttttagtg
     3781 ttccatatgc agtttggctc ttagttggat ttttccaaac agttccaatt ggaattgaag
     3841 aagcggctag aattgatggt gcaaataaat ttgttacgtt ttataaagtt gtgctaccga
     3901 ttgtagcacc aggtattgta gcaacagcta tttatacatt tatcaatgct tggaatgaat
     3961 tcctgtatgc cttgattttg attaacaata caggaaagat gacagtagca gtagcccttc
     4021 gttcacttaa tggttcagaa atactagact ggggagatat gatggcagcg tctgttattg
     4081 tagttcttcc atcaattatt ttcttctcta tcatccaaaa taagattgca agtggattat
     4141 cagaaggatc tgtgaagtag acgaaagaag gaaaaaaatg aataaaagag gtctttattc
     4201 aaaactagga atttctgttg taggcattag tcttttaatg ggagtccccg ctttgattca
     4261 tgcgaatgaa ttaaactatg gtcaactgtc catatctcct atttttcaag gaggttcata
     4321 tcaactgaac aataagagta tagatatcag ccctttgtta ttagataaat tgtctggaga
     4381 tagtcagaca gtagtaatga aatttaaagc agataaacca aactctcttc aagctttgtt
     4441 tggcctatct aatagtaaag caggctttaa aaataattac ttttcaattt tcatgagaga
     4501 ttctggtgag ataggtgtag aaataagaga cgcccaaaag ggaataaatt atttattttc
     4561 tagaccagct tcattatggg gaaagcataa aggacaggca gttgaaaata cactagtatt
     4621 tgtatctgat tctaaagata aaacatacac aatgtatgtt aatggaatag aagtgttctc
     4681 tgaaacagtt gatacatttt tgccaatttc aaatataaat ggtatagata aggcaacact
     4741 aggagctgtt aatcgtgaag gtaaggaaca ttacctcgca aaaggaagta ttgatgaaat
     4801 cagtctattt aacaaagcaa ttagtgatca ggaagtttca aatattccct tgtcaaatcc
     4861 atttcagtta attttccaat caggagattc tactcaagct aactatttta gaataccgac
     4921 actatataca ttaagtagtg gaagagttct atcaagtatt gatgcacgtt atggtgggac
     4981 tcatgattct aaaagtaaga ttaatattgc cacttcttat agtgatgata atgggaaaac
     5041 gtggagtgag ccaatttttg ctatgaagtt taatgactat gaggagcagt tagtttactg
     5101 gccacgagat aataaattaa agaatagtca aattagtgga agtgcttcat tcatagattc
     5161 atccattgtt gaagataaaa aatctgggaa aacgatatta ctagctgatg ttatgcctgc
     5221 gggtattgga aataataatg caaataaagc cgactcaggt tttaaagaaa taaatggtca
     5281 ttattattta aaactaaaga agaatggaga taacgatttc cgttatacag ttagagaaaa
     5341 tggtgtcgtt tatgatgaaa caactaataa acctacaaat tatactataa atgataagta
     5401 tgaagttttg gagggaggaa agtctttaac agtcgaacaa tattcggttg attttgatag
     5461 tagctcttta agagaaaggc ataatggaaa acaggttcct atgaatgttt tctacaaaga
     5521 ttcgttattt aaagtgactc ctactaatta tatagcaatg acaactagtc agaatagagg
     5581 agagagttgg gaacaattta agttgttgcc tccgttctta ggagaaaaac ataatggaac
     5641 ttacttgtgt cctggacaag gtttagcatt aaaatcaagt aacagattga tttttgcaac
     5701 atatactagt ggagaactaa cctatctcat ttctgatgat agtggtcaaa catggaagaa
     5761 atcctcagct tcaattccgt ttaaaaatgc aacagcagaa gcacaaatgg ttgaactgag
     5821 agatggtgtg attagaacat tctttagaac cactacaggt aagatagctt atatgactag
     5881 tagagattct ggagaaacat ggtcggaagt ttcgtatatt gatggaatcc aacaaacttc
     5941 atatggcaca caagtatctg caattaaata ctctcaatta attgatggaa aagaagcagt
     6001 cattttgagt acaccaaatt ctagaagtgg ccgtaaggga ggtcaattag ttgtcggttt
     6061 agtcaataaa gaagatgata gtattgattg gaaataccac tatgatattg atttgccttc
     6121 gtatggttat gcctattctg cgattacaga attgccaaat catcacatag gtgtactgtt
     6181 tgaaaaatat gattcgtggt cgagaaatga attgcattta agcaatgtag ttcagtatat
     6241 agatttggaa attaatgatt taacaaaata aaggagaaaa acatggttaa atacggtgtt
     6301 gttggagcag ggtattttgg agctgaattg gctcgctata tgcaaaagaa tgatggagca
     6361 gagattactc ttctctatga tccagataat gcagaggcga ttgcagaaga attgggagca
     6421 aaagtagcaa gttccttaga tgagttggtt tctagcgatg aagtagattg tgttatcgtc
     6481 gcaactccaa ataatcttca taaggaaccg gttattaagg ctgcacagca tggtaaaaat
     6541 gttttctgtg aaaaaccaat tgcgctttct tatcaagatt gtcgcgagat ggtagatgcg
     6601 tgtaaagaaa acaatgtaac ctttatggca ggacatatta tgaatttctt taatggtgtt
     6661 catcatgcaa aagaactcat taatcaagga gttatcggag acgttctata ttgtcataca
     6721 gctcgtaatg gttgggaaga acaacaaccg tcagtatcat ggaaaaaaat tcgtgaaaaa
     6781 tcaggtggtc acttgtatca ccacatccat gaattggatt gcgttcaatt ccttatgggg
     6841 ggcatgcctg aaactgtaac catgacaggt ggaaatgtgg cccatgaagg tgaacatttc
     6901 ggtgatgaag atgatatgat ttttgtcaat atggaatttt ctaataagcg ttttgccttg
     6961 ttagaatggg gttcagctta tcgttggggt gaacattatg tcttaatcca aggaagcaaa
     7021 ggtgccatcc gcttagactt attcaactgt aaaggaactc ttaagctaga tgggcaagaa
     7081 agctatttct tgattcacga atcgcaagaa gaagatgatg atcggactcg tatctatcat
     7141 agtacagaga tggatggagc aattgcttat ggtaaaccag gtaaacgtac tccattatgg
     7201 ctatcatctg tcattgataa agaaatgcgc tatctgcatg agattatgga aggagctcca
     7261 gtatcagaag aatttgcaaa acttttgaca ggtgaagctg ccctagaagc aattgctact
     7321 gcagatgctt gtacccagtc tatgtttgaa gatcgcaaag taaaattgtc agaaattgta
     7381 aaataaattt tggtattctc ctatttatag gtcgacttgc tcctctgaaa gtacttttag
     7441 aggagctgtt tgactttgct agtttttgaa actgaaatct attatactac aaactattga
     7501 aagcgtttta attttaaggt ataataatct catagaaata aagaaaagga ggaaagagga
     7561 tgccacagat tagcaaagaa gccttgattg agcaaatcaa agatggaatc atcgtttctt
     7621 gtcaggctct tcctcatgaa ccgctttata cagaagcggg aggggtgatt cccttgctgg
     7681 tcaaagcggc tgagcaaggt ggagcagtcg gtatccgagc aaacagtgtt cgcgatatca
     7741 aggaaattaa ggaagtcact aaacttccaa tcattgggat tatcaaacgt gattatccac
     7801 ctcaggaacc cttcatcacg gctactatga aagaagttga tgaattggca gaactggaca
     7861 tcgaggtgat tgctctggat tgtaccaagc gtgaacgcta cgatggtttg gaaattcaag
     7921 agttcattcg tcaggttaag gagaaatatc ctaatcagct tttgatggct gatactagta
     7981 tcttcgaaga agggctagca gctgtagaag caggaattga ctttgtcgga acaaccttat
     8041 caggctacac atcctacagt ccaaaagtag acggtccaga ttttgaattg attaagaaac
     8101 tctgtgatgc cggtgtagat gtcattgcag aaggaaaaat tcatacacca gaacaagcca
     8161 aacaaatcct tgaatatgga gtgcgaggca tcgttgttgg tggcgccatt actagaccaa
     8221 aagagattac agaacgcttc gttgctagtc ttaaataaga tgtgaggggg agttttatgt
     8281 ttaaagtttt acaaaaagtt ggaaaagctt ttatgttacc tatagctata cttcctgcag
     8341 caggtctact tttggggatt ggtggtgcac tttcaaaccc aaccacgata gcaacttatc
     8401 caatactaga caatagtatt tttcaatcaa tattccaagt aatgagctct gcaggagagg
     8461 ttgtattcag taatttgtca ctacttctct gtgtgggatt atgtattggc ttagcgaaac
     8521 gagataaagg aaccgctgcg ttagcaggag taactggtta cttagttatg actgcaacga
     8581 tcaaagcttt agtaaaactt tttatggcag aaggatctgc aattgatact ggagttattg
     8641 gagcattagt tgtcggaata gttgccgtat atttgcacaa ccgatataac aatattcaat
     8701 taccttccgc tttaggattc tttggaggtt cacgcttcgt tcctattgtt acatcgttct
     8761 cttctatctt gattggcttt gtcttctttg ttatttggcc acctttccaa caacttcttg
     8821 tttctacagg tggatatatt tctcaggcgg gtccaattgg aacttttcta tatggatttt
     8881 taatgagact ttctggagca gtaggcttac atcatataat ttaccctatg ttttggtata
     8941 ctgaacttgg tggtgttgaa actgttgcag gacaaacagt ggttggagct caaaaaatat
     9001 tttttgctca attagccgat ttggcccatt ctggattatt tacagaagga acaaggtttt
     9061 ttgcaggtcg tttctcaaca atgatgttcg gtttaccggc tgcctgttta gcgatgtacc
     9121 atagtgttcc taaaaatcgt cgtaaaaaat acgcgggttt gttttttgga gttgctttaa
     9181 catcttttat taccggtatt acagaaccaa ttgaatttat gtttctattc gtcagtccgg
     9241 ttctatatgt tgttcacgca ttccttgatg gtgttagctt ctttattgca gacgtcttaa
     9301 atatttcaat aggaaacaca ttttcaggag gtgtaatcga tttcacttta tttggaattt
     9361 tgcaggggaa cgctaagacg aattgggttc ttcagattcc atttggactt atttggagtg
     9421 ttttgtatta tattattttt agatggttca ttactcaatt caacgttcta acgccaggac
     9481 gaggagaaga agtagattct aaagaaattt ctgaatccgc agattcaact tcaaatattg
     9541 cagattattt aaaacaggat agcctacaaa ttatcagagc cttgggtgga tcaaataata
     9601 tagaagatgt agatgcttgt gtgacacgtt tacgtgtagc tgtaaaagaa gttaatcaag
     9661 ttgataaagc acttttaaaa caaattggtg cagttgatgt cttagaagtg aagggtggca
     9721 ttcaagcaat ctatggagca aaagcaatct tatataaaaa tagtattaat gaaattttag
     9781 gtgtagatga ttaagtactt actgacttaa taaaaaacag aggagagtga tggatgagta
     9841 ggatgaaatg aaatcgcata caagaaataa agaactcatt atccaagttg gatacgctta
     9901 ttacatagga gaatacaaat gaaatttaga aaattagctt gtacagtact tgcgggtgct
     9961 gcggttcttg gtcttgctgc ttgtggcaat tctggcggaa gtaaagatgc tgccaaatca
    10021 ggtggtgacg gtgccaaaac agaaatcact tggtgggcat tcccagtatt tacccaagaa
    10081 aaaactggtg acggtgttgg aacttatgaa aaatcaatca tcgaagcgtt tgaaaaagca
    10141 aacccagata taaaagtgaa attggaaacc atcgacttca agtcaggtcc tgaaaaaatc
    10201 acaacagcca tcgaagcagg aacagctcca gacgtactct ttgatgcacc aggacgtatc
    10261 atccaatacg gtaaaaacgg taaattggct gagttgaacg acctcttcac agatgaattt
    10321 gttaaagatg tcaacaatga aaacatcgta caagcaagta aagctggaga caaggcttat
    10381 atgtatccga ttagttctgc cccattctac atggcaatga acaagaaaat gttagaagat
    10441 gctggagtag caaaccttgt aaaagaaggt tggacaactg atgattttga aaaagtattg
    10501 aaagcactta aagacaaggg ttacacacca ggttcattgt tcagttctgg tcaaggggga
    10561 gaccaaggaa cacgtgcctt tatctctaac ctttatagcg gttctgtaac agatgaaaaa
    10621 gttagcaaat atacaactga tgatcctaaa ttcgtcaaag gtcttgaaaa agcaactagc
    10681 tggattaaag acaatttgat caataatggt tcacaatttg acggtggggc agatatccaa
    10741 aactttgcca acggtcaaac atcttacaca atcctttggg caccagctca aaatggtatc
    10801 caagctaaac ttttagaagc aagtaaggta gaagtggtag aagtaccatt cccatcagac
    10861 gaaggtaagc cagctcttga gtaccttgta aacgggtttg cagtattcaa caataaagac
    10921 gacaagaaag tcgctgcatc taagaaattc atccagttta tcgcagatga caaagagtgg
    10981 ggacctaaag acgtagttcg tacaggtgct ttcccagtcc gtacttcatt tggaaaactt
    11041 tatgaagaca aacgcatgga aacaatcagc ggctggactc aatactactc accatactac
    11101 aacactattg atggatttgc tgaaatgaga acactttggt tcccaatgtt gcaatctgta
    11161 tcaaatggtg acgaaaaacc agcagatgct ttgaaagcct tcactgaaaa agcgaacgaa
    11221 acaatcaaaa aagctatgaa acaatagtcc ttagttattc tataaaaagt agttttttaa
    11281 agaacctaag agtgtatacc cccttttccc tctacacaga tagtgtaaga aaagggggct
    11341 tttgtttaaa atgtaagaaa ctgtcacgaa attaaaatga agttcttaca taagcgaacc
    11401 ataaaaaatt tcattttgat tttaaaacag ttcaagaaag tcaaaaaatt attctatttg
    11461 aaagagaggt gccgactgtg aaagtcaata aaatccgtat gcgggaaaca gtgatttcct
    11521 acgctttcct agcaccagta ttattcttct ttgtcatctt tgtgttggct ccgatggtga
    11581 tgggcttcat tacaagtttc tttaactact caatgactaa atttgagttt gtgggcttag
    11641 ataactatat ccgtatgttt aaagatcctg tctttacaaa atctctgatt aacacagtta
    11701 ttttggttat tggatctgta ccagttgttg ttctattctc actctttgta gcatctcaga
    11761 cctatcatca aaatgtcatt gccagatcct tctaccgttt cgtcttcttc cttcctgttg
    11821 taacgggtag tgttgccgtg acagttgttt ggaaatggat ttatgacccg ctatcaggga
    11881 ttctaaactt tgtccttaag tcaagccaca tcatcagcca aaacatttct tggttgggag
    11941 ataaaaactg ggcattgatg gcgattatga ttattctctt gaccacttca gttggtcagc
    12001 ccatcatcct ttatatcgct gccatgggga atattgacaa ttcactggtt gaagcggcgc
    12061 gtgttgatgg cgcaactgag tttcaagttt tttggaagat taaatggcca agccttcttc
    12121 caacaactct ttatattgca atcatcacaa caattaactc attccagtgt ttcgccttga
    12181 ttcagctttt gacatctggt ggtccaaact actcaacaag taccttgatg tactaccttt
    12241 acgaaaaagc cttccaattg acagaatacg gctatgccaa cacaattggt gtcttcttgg
    12301 cagtcatgat tgctatcgta agctttgttc aatttaaagt acttggaaac gacgtagaat
    12361 actaaagaaa ggagacagct atgcaatcta cagaaaaaaa accattaaca gcctttactg
    12421 ttatttcaac aatcattttg ctcttgttga ctgtgctgtt catctttcca ttctactgga
    12481 ttttgacagg ggcattcaaa tcacaacctg atacaattgt tattcctcct cagtggttcc
    12541 ctaaaatgcc aaccatggaa aacttccaac aactcatggt gcagaaccct gccttgcaat
    12601 ggatgtggaa ctcagtattt atctcattgg taaccatgtt cttagtttgt gcaacctcat
    12661 ctctagcagg ttatgtattg gctaaaaaac gtttctatgg tcaacgcatt ctatttgcta
    12721 tctttatcgc tgctatggcg cttccaaaac aagttgtcct tgtaccattg gtacgtatcg
    12781 tcaacttcat gggaatccac gatactctct gggcagttat cttgcctttg attggatggc
    12841 cattcggtgt cttcctcatg aaacaattta gtgaaaatat tcctacagag ttgcttgaat
    12901 cagctaaaat cgacggttgt ggtgagattc gtaccttctg gagtgtagcc ttcccgattg
    12961 tgaaaccagg gtttgcagcc cttgcaatct ttaccttcat caatacttgg aatgactact
    13021 tcatgcaatt ggtaatgttg acttcacgta acaatttgac catctcactt ggggttgcga
    13081 ccatgcaggc tgaaatggca accaactatg gtttgattat ggcaggagct gcccttgctg
    13141 ctgttccaat cgtcacagtc ttccttgtct tccaaaaatc cttcacacag ggtattacta
    13201 tgggagcggt caaaggataa tactctgcga aaatcgaatg caaactacgt cagcttcacc
    13261 ttgccatact taagtattgc ctgcggttag cttcctagtt tgttcttcaa ttttcattga
    13321 gtataggaaa atcaatctat caagatacag aagtatattt tatagattta gagaatatag
    13381 aggttataag tgtctacaaa atggagggta tgcagttact ttatgaagtt ttgtcagaca
    13441 cttatcaact taagaatggt tttagttaac tatcagaaac gaaggaaaga gtatgatttt
    13501 tgacgatttg aaaaacatca ccttttacaa agggattcat cccaatttag acaaggctat
    13561 cgactacctc taccaacatc gtaaagattc attcgaatta ggaaagtatg agattgatgg
    13621 agataaagtc tttctagttg ttcaggaaaa tgtcctcaat caagttgaga ataatcaatt
    13681 tgaacaccat aagaactatg cagatttgca tttgctgata gaagggcatg aatattcgag
    13741 ctacggttca cgtatcaaag acgaggcagt agcattcgac gaagcgagtg acattggctt
    13801 tgttcattgt catgaacact acccgctctt gttgggttat cacaattttg cgattttctt
    13861 cccaggtgag ccacatcagc caaatggtta tgcaggcatg gaagaaaagg ttcgaaaata
    13921 tctctttaaa atcttgattg attaaaaata ggatgaattg tttttttgta aagctttgat
    13981 aatactctac catgaaattg atctttgtga ggtagagaaa tgagaataaa atatttaaaa
    14041 attggtatct tctaagtatg ctgcaagagc tagtttctta gatggacagg ggattacagt
    14101 tgatgagatg tcctggataa ttaggggcat tgtgaatgga ttgattggta gatacataaa
    14161 attaggtact tatgcagcta agtatggtat tagtatggcg cgctcgatct taagtagggc
    14221 agctgcaact gcggcagcaa gagtaagatt attgactaag atttttggat ggattttacg
    14281 aatagctgtg ctcgcagctg atgtatatgg taattttgcc aacaatattg ctgcagcttg
    14341 ggatgcacat gataaaatcc ctaacaatgg tcgtataaac ttttaaaatg cgagaatgaa
    14401 agcactttgt atttttttat tgtatatgtt agcttggaca gtgcttgcaa tgataatccg
    14461 tggagggcta gatggatttg atgaacacac ttggcgtaat attttagcta gtgcgttgtc
    14521 ctcgctatac atacattata tatccataga tagaaaaaga aaaaagtcca aaagaaaaaa
    14581 gaaaaaatag atttgttcat ggtagggact tatgaaagct ttactaacaa aaaagaaaac
    14641 agtttacaaa gaaaaatgat ggaggagcaa acatggcaca aaaaggagta agccttatca
    14701 aggcagcatt tgatacagat aactttctca tgcgttttag tgagaaagtc ttggacatcg
    14761 tgacagccaa tcttcttttt gtcgtctctt gtttgcccat cgtgacgatt ggagtggcta
    14821 aaatcagcct ctacgagacc atgttcgaag ttaagaagag cagacgggtg cctgttttta
    14881 aaatctatct aagatctttc aagcaaaatc tgaaactagg tcttcagctg ggtttaatgg
    14941 agttaggaat tgtgtttctt accctttcag atctctatct tttctggggt caaacagctc
    15001 tgcccttcca attgctgaaa gccatttgtc taggcattct gattttcctt acgattgtga
    15061 tgttggctag ctatccaatc gcggcacgtt atgatttaac ttggaaagaa gtgttacaaa
    15121 aagggcttat cttggctagt tttaactttc cttggttctt cctcatgtta gccattcttg
    15181 tcctcattgt gatggttctt tatctgtccg ccttcagtct actcttaggt ggctcagtct
    15241 tcctactttt tgggtttgga ctattggtct ttatccagac tggattgatg gagaaaattt
    15301 tcgcaaaata ccaataggag ccttatttct gaaactactt tcaaaggctc caaacgctat
    15361 tctataagcg agaaactaaa atctaagtgt ctataagata ttgaaagcct tttcggtaaa
    15421 gtgtatacta aagaagtaaa gaaaaatctg ctttagcaga aaataaaaat aataggagaa
    15481 aatctatgtc agatttgaaa aaatacgaag gtgtcattcc agccttctac gcatgttatg
    15541 atgatcaagg agaagtaagc ccagaacgta cgcgtgcctt ggttcaatac ttcattgata
    15601 aaggtgttca aggtctttat gtcaatggtt cttctggtga atgtatctac caaagcgttg
    15661 aagatcgcaa gttgattttg gaagaagtca tggcggtagc caaagataaa ttgaccatta
    15721 ttgcccatgt tgcttgcaat aatactaaag atagtatgga acttgctcgc catgctgaaa
    15781 gcttgggagt agatgctatt gcaacgattc caccaattta tttccgcttg ccagaatact
    15841 cagttgccaa atactggaac gatatcagtt ctgcagctcc aaacacagac tacgtgattt
    15901 acaacattcc tcaattggca ggggttgctt tgactccaag cctttacaca gaaatgttga
    15961 aaaatcctcg tgttatcggt gtgaagaact cttctatgcc agttcaagat atccaaacct
    16021 ttgtcagcct tggtggagaa gaccatatcg tctttaatgg tcctgatgag cagttcctag
    16081 gaggacgcct catgggggct agggctggta tcggtggtac ttatggtgct atgccagaac
    16141 tcttcttgaa actcaatcag ttgattgcgg ataaggacct agaaacagcg cgtgaattgc
    16201 agtatgctat caacgcaatc attggtaaac tcacttctgc tcatggaaat atgtacggtg
    16261 tcatcaaaga agtcttgaaa atcaatgaag gcttgaatat tggatctgtt cgttcaccat
    16321 tgacaccagt gactgaagaa gatcgtccag ttgtagaagc ggctgctgcc ttgattcgtg
    16381 aaaccaagga gcgcttcctc taatctaaaa ggaggtattt atgacatatt acgttgcaat
    16441 tgatatcggt ggaaccaaca tcaagtatgg tttggttgat caagaggggc aacttcttga
    16501 atcgcatgaa atgccaactg aggcgcataa gggtggacct catatcttac aaaagaccaa
    16561 agatatcgta gctagttatt tagaaaaagg cccagtagca ggtgttgcca tatcttctgc
    16621 tgggatggtg gatccggata agggtgagat tttctatgct gggccgcaaa tccctaacta
    16681 cgcaggcacc cagttcaaaa aggaaatcga agaaagcttt actattcctt gtgagattga
    16741 aaatgatgtc aactgtgcag gtcttgctga ggcagtatct ggttcaggca agggagcaag
    16801 tgtgacactt tgcttgacca ttggaacagg tatcggtggt tgcttgatta tggataggaa
    16861 agtcttccat ggttttagca attcagcctg tgaagtcggg tatatgcata tgcaggatgg
    16921 agcttttcag gatctcgctt ctacaacagc tttagtgaaa tatgtagctg aagcccatgg
    16981 agaagatgtt gatcagtgga atggccgacg tatctttaag gaagctactg aaggaaacaa
    17041 aatctgcatg gaaggcattg accgtatggt tgactatcta ggaaaaggtc tggcaaatat
    17101 ttgctacgtt gccaatccag aagtggttat tcttggtggt ggtatcatgg ggcaagaggc
    17161 tatcctcaaa cctaagatcc gcacagcctt gaaagaggct ttggtaccaa gtttagcaga
    17221 aaaaacacga ttagaatttg cccatcacca aaatacagca gggatgttgg gtgcatatta
    17281 tcattttaag acaaaacaat cctagtttgg ctcagccaaa ctaggatttt cttacacgtt
    17341 tttgtctacg atagccgttg agttttttat tttcccagta gctattaaag attttttcct
    17401 tgctttcgcg attgatttcc aaaaagtagg cataaatcaa atcgataaag aagagcatag
    17461 gaagttgagc ggatattcgt tggatatagg agggttggct gtgggtggct acaagaacag
    17521 tctctgtata ggtctggcta tctttattgg gaacacttgt aaagagtaca gtctttgccc
    17581 ccatctcctt ggcatctaat agactatcta aaatagaagg agttgagcct gaaagtgaga
    17641 agcccagtac tagacaattt ccatccatga tgctggttgt ccaggcaaag ccgtcttggt
    17701 ctgtcaaagc ttcgcagacc acacctagtc gcataaaacg taatttcatt tcacgggcga
    17761 cgaggccaga actccctgtt ccaaagaagt agatacgctc agcatcttcg attagctggg
    17821 caattcgctc tagttggatt tcgtcaatca agtcttgtgt ttgttccctc atattgctat
    17881 aacttctgag gactcgtttg gtcagtggac tgtgcttgga gacttggttg gcttgatttt
    17941 ctgcctgatg ttggtattgg aaaataaatt ctcggtagcc agtaaagcca cactttttag
    18001 caaagcgggt caaagcagct tgagaaatat gtaatttttg ggtgacttgt tgagaagata
    18061 aatcatctgt aatcgtttca gcttgcaaaa aatagcgagc gatttcttgc tctaggtctg
    18121 tcatttcttc aaaatgtgaa tcaatgatag ttgcgatatc tggtttgtcc atagggaaag
    18181 ctcctttaca tgagtcatac tggaagacta gatcagggaa tagtcacatt tcattataac
    18241 acataatata aggatagata aataaaaacg catttctgtt ttaaaaacga aaaaatcgaa
    18301 aaagcttctt tcttttccat aattttctac tcaaattgtg gtacaattaa gagtaagatt
    18361 ttaagttaga aatgagactg atttgtatga gaaaatttaa cagccattcg attccgattc
    18421 ggcttaattt attgttttca atcgtcattt tactctttat gaccattatt ggtcgtttgt
    18481 tgtatatgca ggttttgaac aaggattttt acgaaaaaaa gctagcctca gctagtcaga
    18541 ccaagattac aagcagttca gcccgtgggg aaatttatga tgctagtgga aaacctttgg
    18601 tagaaaatac gttaaagcag gttgtttcct ttacgcgtag caataaaatg acggctacag
    18661 acttaaaaga aacagctaaa aagttactga cttatgtgag catcagttct ccaaatttga
    18721 cagaacgcca gctggcggat tactatttgg ctgatcctga aatctataaa aaaacagtgg
    18781 aagctctccc aagtgagaaa cgcttggatt cagatggcaa tcgtctatcc gaatcagaac
    18841 tgtataacaa tgcggtcgat agtgtccaaa caagtcaact aaactataca gaggatgaaa
    18901 agaaagaaat ctatcttttt agtcagttaa atgctgttgg aaactttgcg acaggaacca
    18961 ttgctacaga tcctctaaat gattctcagg tggctgttat tgcctctatt tcaaaggaga
    19021 tgcctggcat tagtatttct acttcttggg atagaaaggt tttggaaact tccctttctt
    19081 ctatagttgg gagtgtatcc agtgaaaaag ctggtctccc agcggaagaa gcagaagcct
    19141 atcttaaaaa aggctattct ctaaatgatc gtgtaggaac ctcctatttg gaaaagcaat
    19201 atgaagagac cttacaagga aaacgctcgg taaaagaaat ccatctggat aaatatggca
    19261 atatggaaag cgtggataca attgaggaag gtagtaaggg aaacaatatc aaactgacca
    19321 ttgatttggc tttccaagat agcgtggatg ctttactgaa aagttatttc aattccgagc
    19381 tagaaaatgg tggagccaag tattctgaag gtgtctatgc agtcgccctt aacccaaaaa
    19441 caggtgcggt tttgtctatg tcagggatta aacatgactt gaaaacggga gagttgacgc
    19501 ctgattcctt gggaacggta accaatgtct ttgttccagg ttcggttgtc aaggcggcga
    19561 ccatcagctc aggttgggaa aatggagtct tgtcaggaaa ccagaccttg acagaccagt
    19621 ccattgtctt ccaaggttca gctcccatca attcttggta tactcaggct tacggttcat
    19681 tccctatcac agcggtccaa gctctggagt attcatcaaa tacctatatg gtccaaacag
    19741 ccttaggtct tatggggcaa acctatcaac ccaatatgtt tgtcggcacc agcaatctag
    19801 agtctgctat ggagaaactg cgttcaacct ttggcgaata tggcttgggt actgcgacag
    19861 gaattgacct accagatgaa tctactggat ttgttcccaa agagtatagc tttgctaatt
    19921 acattactaa tgcctttggg cagtttgata actatacgcc gatgcagttg gctcagtatg
    19981 tagcaactat tgcaaataat ggtgttcgtg tggctcctcg tattgttgaa ggcatttatg
    20041 gtaataatga taagggagga ctgggtgact tgattcagca actgcaaccg acagagataa
    20101 ataaggtcaa tatatccgac tccgatatga gcatcttgca ccaaggtttt tatcaggttg
    20161 cccatggtac tagtggattg acaactggac gtgccttttc aaatggcgcc ttggtatcca
    20221 ttagcggaaa aacaggtaca gccgaaagct atgtggcaga tggtcagcaa gcaaccaata
    20281 ccaatgcggt ggcctatgcc ccatctgata atccccaaat cgctgttgca gtggtctttc
    20341 ctcataatac caatctaaca aatggtgtag gaccttccat tgcgcgtgac attatcaatc
    20401 tgtatcaaaa ataccatcca atgaactaga aaggaaatta tgctttatcc aacacctatt
    20461 gccaagttga ttgacagtta ttctaagtta ccaggtatcg ggattaagac ggctacgcgt
    20521 ctggcctttt atacgattgg gatgtctgct gatgatgtca atgaatttgc aaaaaatctc
    20581 ctttctgcta agagagaatt gacatattgt tctatttgtg gacgtttgac agacgacgat
    20641 ccttgttcta tctgtactga tccgactcgt gaccagacaa caattttagt tcttgaggat
    20701 agtagagatg tggcagccat ggaaaatatc caagaatacc atggactcta tcatgtcctt
    20761 catggcctca tttctcctat gaatggtatc agtccggacg atatcaatct caagagcctt
    20821 atgactcgtc ttatggatag tgaagtttca gaagtgattg tggcgactaa tgctacagcg
    20881 gatggtgaag cgacttccat gtatctttca cgtttgctca agccggctgg tatcaaggtt
    20941 acgcgtctag cacgaggtct cgctgtggga gcggacattg agtatgcgga cgaagtgaca
    21001 ctcttacgag cgattgaaaa tcggacagag ttgtaagtgt aggcaaattt acgaactcca
    21061 ttcatttata aaaaatcaaa gaggctgaaa atcgttccta tcggcctctt tttgtatagt
    21121 gtgatgagta ggatcaggtt caagttttaa aaaaccaagc aaatatgata tactaaagag
    21181 cgagtattct agtagaatta ggacaaataa tatgaaacaa acgattattc ttttatatgg
    21241 tggacggagt gcggaacgcg aagtctctgt cctttcagct gagagtgtca tgcgtgcggt
    21301 caattacgac cgtttcacag tcaagacttt ctttatcagt cagtcaggtg actttatcaa
    21361 aacacaggaa tttagtcatg ctccggggca agaagaccgt ctcatgacca atgaaaccat
    21421 tgattgggat aagaaagttg caccaagtgc tatctacgaa gaaggtgcag tggtctttcc
    21481 agtccttcac gggccaatgg gagaagatgg ctctgttcaa ggattcttgg aagttttgaa
    21541 aatgccttac gttggttgca acattttgtc atcaagtctt gccatggata aaatcacgac
    21601 taagcgtgtt ctggaatctg ctggtattgc ccaagttcct tatgtggcta tcgttgaagg
    21661 cgatgatgtg actgctaaaa tcgctgaagt ggaagaaaaa ttggcttatc cagtcttcac
    21721 taagccgtca aacatggggt ctagtgtcgg tatttctaag tctgaaaacc aagaagaact
    21781 ccgtcaagcc ttaaaacttg ccttccgata tgacagccgt gtcttggttg agcaaggagt
    21841 gaatgcccgt gaaattgagg ttggcctctt gggtaactac gatgtcaaga gcacgctacc
    21901 tggagaagtt gtcaaggacg ttgcctttta tgactacgat gccaagtata ttgataacaa
    21961 gattactatg gatattcctg ccaaaatcag tgatgatgtg gtggctgtca tgcgtcaaaa
    22021 tgcagaaaca gccttccgtg ccattggtgg ccttggtcta tctcgttgcg atttcttcta
    22081 tacagataag ggagagattt ttctcaacga gctcaatacc atgccaggtt ttacccagtg
    22141 gtctatgtac ccactacttt gggacaatat ggggatcagc tacccagaac taatcgagcg
    22201 tttggttgac cttgccaagg aaagttttga caagcgcgaa gcgcatttga tataaaatga
    22261 aagagagggt agaagccaga accatcactg cacggtgact agagttctcg gacttcagcc
    22321 ctttttaaag gagtagaaat gaaattaaca atccatgaaa ttgcccaagt tgttggagcc
    22381 aaaaatgata tcagtatctt tgaggacacc cagttagaaa aagctgagtt tgatagtcgt
    22441 ttgattggaa ctggagattt atttgtgcca cttaaaggtg cgcgtgatgg ccatgacttt
    22501 attgaaacag cctttgaaaa tggtgcagca gtaaccttgt ctgagaaaga ggtctcaaat
    22561 catccttaca ttctagtaga tgatgttttg acagcctttc aatccctagc atcctactat
    22621 cttgaaaaaa cgactgttga tgtctttgct gttacaggtt caaatggcaa gacaacgact
    22681 aaggatatgt tggcgtattt actgtcaaca agatacaaga cctacaaaac acaaggcaat
    22741 tacaataatg agattggcct tccttacaca gttctccaca tgcctgaagg aacagaaaag
    22801 ttggtcttgg agatggggca ggaccacttg ggagatattc atctcttgtc tgaattggct
    22861 cgtccaaaaa cagccatcgt gaccttggtt ggagaagctc atttggcctt tttcaaagat
    22921 cgttctgaga ttgctaaagg aaaaatgcaa attgcagacg gaatggcttc aggttccttg
    22981 cttttagcgc cggctgaccc tatcgtagag gactatttgc caactgataa aaaggtggtt
    23041 cgttttggtc aaggagcaga gctggaaatt actgacttgg ttgagcgcaa agatagtctg
    23101 accttcaatg ccaatttctt agagcaagtc cttgatttgc cagtaactgg caagtataat
    23161 gcgacaaatg ctatgattgc atcctatgtt gccttgcaag aaggagtttc agaggagcaa
    23221 attcatcagg ccttccaaga tcttgaattg acgcgtaacc gtaccgaatg gaagaaagca
    23281 gccaatggag cagatatcct gtctgatgta tacaatgcca atccaactgc tatgaagttg
    23341 attttagaga ctttctctgc cattccagcc aatgaaggtg gcaagaaaat tgcggtttta
    23401 gcggacatga aggaactggg taaccagtca gttcaacttc ataatcagat gattttgagc
    23461 ctttctccag atgtgcttga taccgtgatt ttctatggag aagacattgc tgaattagcc
    23521 caattggcca gtcaaatgtt cccaattggc cacgtttact acttcaagaa aacagaagac
    23581 caagaccaat ttgaagacct agtcaagcag gtcaaggaaa gcctcagtgc caatgatcaa
    23641 atcttgctca agggctctaa ctctatgaac ctagctatgt tggtagaaag tttagaaaat
    23701 gaaaccaagt gattttgtta agtatctgca aagaatgatt gcccttacag atactggctt
    23761 aacctttaca aaagatcctt ttgaccgtga gcgctacgag gacttgcgaa gtctgttatc
    23821 tgaaatgttg aatcagggat cagacctgga tgcagaagaa gtagcagaag tcttgaaacc
    23881 aacttcagct tatgcgactc ccttaatgga cgtccgtgct tggattgttg aggatgagaa
    23941 gatttgtctg gttaggggac aaggagaggg tagttgggct ttgccaggtg gctttggtga
    24001 agtcggctat tctccaactg aaaatattct caaggaaatt gaagaagaaa ccggttttaa
    24061 agccaaagtt gaaagactgc tagctgtttt tgatatcaat cgtttccaac tacagagcaa
    24121 acaatatgca aagtttgttt ttgaatgtaa gcttctcgat ggacaattcc aagaaaatca
    24181 agaaattgca gaccttcaat tttttgccat tgatcaactg ccagtcttat ctgaaaaacg
    24241 cattaccaag gagcaaatag agattctttg gcaggtttat caaggtcaga gggagcaata
    24301 tcttgactaa gaagatgatt atcgtatttt taaatccatt tttaacaact agcatggtat
    24361 aataatatgc aggaaaattt tgaattatga ggaagactag atgaatttat gggatatttt
    24421 ctttacgact caggcaacca agccgcccaa atttgacctt ttttggtatg ttagcctatt
    24481 tacgctctta gccttaacct tttatacagc ccatcgctat cgtgaaaaga aggtttacca
    24541 acgatttttc caaatcttgc agactgttca gttaatcctt ctttatggtt ggtactgggt
    24601 caatcatatg ccactgtcag aaagcctacc cttttaccat tgccgtatgg ctatgtttgt
    24661 ggtactcttg cttcctggtc aatccaaata taaacaatac tttgcattat tgggaacatt
    24721 tgggacatta gcagcctttg tttatccagt gccagatgct tacccttttc cacatatcac
    24781 cattctatcc tttatctttg gtcatttagc actcttgggg aactctctag tttatctatt
    24841 gagacagtat aatgcgcgat tgctggatgt gaagggaatt tttctcatga cctttgccct
    24901 aaatgccttg atttttgtgg tcaatttggt gacaggtggc gattacggat ttttgacaaa
    24961 accgccattg gttggggatc acggtctagt agctaattat ttacttgttt caattgtgct
    25021 ggtagctact atcagtttga ctaagaaaat cttagaattc tttttagctc aagaagcaga
    25081 aaaaatgatt gtaaaggaag cttaacacag agctttcttt tttgctctta gagagttttt
    25141 acaatcagct tataaaataa gaatttctga atagacaaac tcaaaaaatg gctgggaaat
    25201 ttaggaaaaa agcaagcacg attaaatttt ttgtgttata atattttgtg aatagctatg
    25261 cctatgttta gctatggaat aatacgaagt gcgaaacttg gaagatagag aggaagcgat
    25321 gtaatggcta gagaaggctt ttttacaggt ctagatattg gaacaagctc tgtcaaggtg
    25381 cttgtggccg agcagagaaa tggtgaatta aatgtaattg gcgtgagtaa tgccaaaagt
    25441 aaaggtgtaa aggatggaat tattgttgat attgatgcag cagcaactgc tatcaagtca
    25501 gccatttccc aagcggaaga aaaggcaggc atttcgatta aatcagtaaa tgtcggcttg
    25561 cctggtaatc ttttgcaggt agaaccaact caggggatga ttccagtaac atctgatact
    25621 aaggaaatta cggatcaaga tgttgaaaat gttgtcaaat cagctttgac aaagagtatg
    25681 acacctgacc gtgaagtcat tacctttatt cctgaagaat ttattgtgga tggtttccaa
    25741 gggattcgtg acccacgtgg catgatgggg gttcgccttg aaatgcgtgg tttgctttat
    25801 acaggacctc gtactatctt gcacaatttg cgtaagacgg ttgagcgtgc aggtgttcag
    25861 gttgaaaatg ttatcatttc accactagca atggttcagt ctgttttgaa cgaaggggaa
    25921 cgtgaatttg gtgctacagt gattgatatg ggggcaggtc aaacgactgt cgctacaatc
    25981 cgtaatcaag aactccagtt cacacatatt ctccaagaag gtggagatta tgtaactaaa
    26041 gatatctcca aggttttgaa aacctctcgc aaattagcgg aaggcttgaa actgaattac
    26101 ggggaagcct atccgcctct tgcaagcaaa gaaaccttcc aagtagaggt tattggagaa
    26161 gtagaagcag tcgaagtgac ggaagcctac ttgtcagaaa ttatttctgc acgaatcaag
    26221 cacatccttg aacaaatcaa gcaagaatta gatagaaggc gtctattgga cctccctggt
    26281 ggtattgtct taatcggtgg gaatgccatt ttaccaggta tggttgagct tgctcaggaa
    26341 gtctttggcg tccgtgtcaa gctttatgtt ccaaatcaag ttggtatccg taatccagcc
    26401 tttgcgcatg tgattagttt atcagaattt gcgggtcaat taacagaagt taatcttttg
    26461 gctcagggag cgataaaagg tgagaatgac ttaagtcatc agccaattag ttttggtggg
    26521 atgctgcaaa aaacagctca gtttgtacaa tcaacgcctg ttcaaccagc tcctgctcca
    26581 gaagtagagc cggtggcgcc tacagaacca atggcggatt tccaacaagc ttcacaaaat
    26641 aaaccgaaat tagcagatcg tttccgtggc ttgatcggaa gcatgtttga cgaataaaga
    26701 ggaaaaataa attatgacat tttcatttga tacagctgct gctcaagggg cagtgattaa
    26761 agtaattggt gtcggtggag gtggtggcaa tgccatcaac cgtatggtcg acgaaggtgt
    26821 tacaggcgta gaatttatcg cagcaaacac agatgtacaa gcattgagta gtacaaaagc
    26881 tgagactgtt attcagttgg gacctaaatt gactcgtggt ttgggtgcag gaggtcaacc
    26941 tgaggttggt cgtaaagccg ctgaagaaag cgaagaaaca ctgacggaag ctattagtgg
    27001 tgccgatatg gtcttcatca ctgctggtat gggaggaggc tctggaactg gagctgctcc
    27061 tgttattgct cgtattgcca aagatttagg tgcgcttaca gttggtgttg taacacgtcc
    27121 ctttggtttt gaaggaagta agcgtggaca atttgctgta gaaggaatca atcaacttcg
    27181 tgagcatgta gacactctat tgattatctc aaacaacaat ttgcttgaaa ttgttgataa
    27241 gaaaacaccg cttttggagg ctcttagcga agcggataac gttcttcgtc aaggtgttca
    27301 agggattacc gatttgatta ccaatccagg attgattaac cttgactttg ccgatgtgaa
    27361 aacggtaatg gcaaacaaag ggaatgctct tatgggtatt ggtatcggta gtggagaaga
    27421 acgtgtggta gaagcggcac gtaaggcaat ctattcacca cttcttgaaa caactattga
    27481 cggtgctgag gatgttatcg tcaacgttac tggtggtctt gacttaacct tgattgaggc
    27541 agaagaggct tcacaaattg tgaaccaggc agcaggtcaa ggagtgaaca tctggctcgg
    27601 tacttcaatt gatgaaagta tgcgtgatga aattcgtgta acagttgttg caacgggtgt
    27661 tcgtcaagac cgcgtagaaa aggttgtggc tccacaagct agatctgcta ctaactaccg
    27721 tgagacagtg aaaccagctc attcacatgg ctttgatcgt cattttgata tggcagaaac
    27781 agctgaattg ccaaaacaaa atccacgtcg tttggaacca actcaggcat ctgcttttgg
    27841 tgattgggat cttcgccgtg aatcgattgt tcgtacaaca gattcagtcg tttctccagt
    27901 cgagcgcttt gaagccccaa tttcacaaga tgaagatgaa ttggatacac ctccattttt
    27961 caaaaatcgt taagtaaatg aatgtaaaag aaaatacaga acttgttttt cgagaagttg
    28021 cagaggctag tctgagtgct catcgagaga gtggttcggt ctctgtcatt gcagttacca
    28081 agtatgtaga tgtaccgaca gcggaagcct tgcttccgct aggtgttcat catatcggtg
    28141 aaaatcgtgt agataagttt ctggaaaaat atgaagcttt aaaagatcga gatgtgactt
    28201 ggcatttgat tggtaccttg caaagacgta aggtgaaaga tgtcattcaa tacgttgatt
    28261 atttccatgc attggactca gtaaagctag caggggaaat tcaaaaaaga agtgaccgag
    28321 tcatcaagtg tttccttcaa gtaaatattt ctaaagaaga aagcaaacac ggtttttcga
    28381 gagaggaact gctggaaatc ttgccagagt tagccagact agataagatt gaatatgttg
    28441 gtttaatgac gatggcacct tttgaggcta gcagtgagca gttgaaagag attttcaagg
    28501 cggcccaaga tttacaaaga gaaattcaag agaaacaaat tccaaatatg cctatgaccg
    28561 agttaagtat gggaatgagt cgtgattata aagaagcgat tcaattcggt tccacttttg
    28621 ttcgtatagg tacatcattt tttaagtagg agagaaccat gtctttaaaa gatagattcg
    28681 atagatttat agattatttt acggaggatg aggattcaag tctcccttat gaaaaaagag
    28741 atgagcctgt gtttacttca gtaaattctt cacaggaacc ggctctccca atgaatcaac
    28801 cttcacagtc ggctggtaca aaagagaaca atatcaccag acttcatgca agacaacagg
    28861 aattggcaaa tcagagtcag cgtgcaacgg ataaggtcat tatagatgtt cgttatccta
    28921 gaaaatatga ggatgcaaca gaaattgttg atttattggc aggaaacgaa agtatcttga
    28981 ttgactttca gtatatgaca gaggtgcagg ctcgtcgttg tttggactat ttggatggag
    29041 cttgtcatgt tttagctgga aatttgaaaa aggtagcttc taccatgtat ttgttgacac
    29101 cagtgaacgt tattgtaaat gttgaagata tccgtttacc agatgaagat caacagggtg
    29161 agttcggttt tgatatgaag cgaaatagag tacgataatg atttttttaa ttcgtatgat
    29221 ttataatgca gtggatattt actccctgat tttggtagcc ttcgctgtca tgtcttggtt
    29281 tccaggtgcc tacgaatcca gtttaggtcg ttggattgta gcgttggtga aaccagtgct
    29341 tgctcccttg caacgcctgc ctttacagat agcgggtctt gatttatctg tttgggttgc
    29401 gattgttttg gttcgatttt taggagaaaa cctagtgcgt tttctggcga tgataggatg
    29461 aataaaggga tttatcagca tttctccata gaagatcgtc catttcttga caagggaatg
    29521 gaatggataa agaaggtaga agatagctat gctccttttt taactccttt tatcaatcct
    29581 catcaggaga agctattaaa gattttggcc aaaacctatg gtcttgcttg tagcagtagt
    29641 ggggaattcg tctcgagtga gtatgttcga gttttattat acccagatta tttccaacca
    29701 gagttttcag attttgaaat atctctccag gaaattgtgt attccaataa atttgaacat
    29761 ttaacgcatg ctaagatttt aggaacagtc atcaatcaat tagggattga acggaaactt
    29821 tttggagata tcctagtaga tgaagaacgg gcgcagatta tgattaatca gcagtttctt
    29881 cttctctttc aagatggact aaagaaaatt ggtcgtatac ctgtttcgct ggaggaacgt
    29941 cctttcaccg agaaaataga taagctagaa cagtatcgag aactggattt atctgtgtct
    30001 agttttcgat tagatgttct tttatcaaat gttttgaaac tatctaggaa tcaagcaaac
    30061 cagttgattg aaaagaaact tgtccaagta aattatcatg tggtagacaa atcagattac
    30121 actgttcaag ttggagactt gattagtgtg agaaaatttg gtcgcttgag attacttcaa
    30181 gataagggac aaacgaaaaa agagaagaaa aaaataaccg tccagttatt attaagtaag
    30241 tgaggaatag aatgccaatt acatcattag aaataaagga caagactttt ggaactcgat
    30301 tcagaggttt tgatccagaa gaagtcgatg aatttttaga tattgtggtt cgtgattacg
    30361 aagatcttgt gcgtgcgaat catgataaaa atttgcgtat taagagttta gaagagcgtt
    30421 tgtcttactt tgatgaaata aaagattcat tgagccagtc tgtattgatt gctcaggata
    30481 cagctgagag agtgaaacag gcggcgcatg aacgttcaaa caatatcatt catcaagcag
    30541 agcaagatgc gcaacgcttg ttggaagaag ctaaatataa ggcaaacgag attcttcgtc
    30601 aagcaactga taatgctaag aaagtcgctg ttgaaacaga agaattgaag aacaagagcc
    30661 gtgtcttcca ccaacgtctc aaatctacaa ttgagagtca gttggctatt gttgaatctt
    30721 cagattggga agatattctc cgtccaacag ctacttatct tcaaaccagt gatgaagcct
    30781 ttaaagaagt ggttagcgaa gtacttggag aaccgattcc agctccaatt gaagaagaac
    30841 caattgatat gacacgtcag ttctctcaag cagaaatggc agaattacaa gctcgtattg
    30901 aggtagccga taaagaattg tctgaatttg aagctcagat taaacaggaa gtggaagctc
    30961 caactcctgt agtgagtcct caagttgaag aagagcctct gctcatccag ttggcccaat
    31021 gtatgaagaa ccagaagtag ctccaatgca tccgataggt ccaacaccag ctacagaaac
    31081 tgttgattca ataccgggat ttgaagcacc gcaagaatct gttacaattt tataagaaat
    31141 attctgagaa caatatctta tccttatatt tccagcgagc aggagatggt gtgagtcctg
    31201 taatccctat tgataagatt atcctctcaa aaactcaagt ctgaagctag taagatttga
    31261 cgtttcccac gttacgggat aagagggaga aagactaaat ctttttccga ataaaggtgg
    31321 taccacgatt ttcgtccttt ttggaagtcg tggtttttaa tttgttatta tttataaagg
    31381 agataccatg aaactcaaag acacccttaa tcttgggaaa actgaattcc caatgcgtgc
    31441 aggccttcct accaaagagc cagtttggca aaaggaatgg gaagatgcaa aactttatca
    31501 acgtcgtcaa gaattgaacc aaggaaaacc tcatttcacc ttgcatgatg gccctccata
    31561 cgctaacgga aatatccacg ttggacatgc tatgaacaag atttcaaaag atatcattgt
    31621 tcgttctaag tctatgtcag gattttacgc accatttatt cctggttggg atactcatgg
    31681 tctgccaatc gagcaagtct tgtcaaaaca aggtgtcaaa cgtaaagaaa tggacttggt
    31741 tgagtacttg aaactttgcc gtgagtacgc tctttctcaa gtagataaac aacgtgaaga
    31801 ttttaaacgt ttgggtgttt ctggtgactg ggaaaatcca tatgtgacct tgactcctga
    31861 ctatgaagca gctcaaattc gtgtatttgg tgagatggct aataagggtt atatctaccg
    31921 tggtgctaag ccagtttact ggtcatggtc atctgagtca gcacttgctg aagcagagat
    31981 tgaataccat gacttggttt caacttccct ttactatgcc aacaaggtaa aagatggcaa
    32041 aggagttcta gatacagata cttatatcgt tgtctggaca acgactccat ttaccatcac
    32101 agcttctcgt ggtttgacgg ttggtgcaga tattgattac gttttggttc aacctgctgg
    32161 tgaagctcgt aagtttgtcg ttgctgctga attattgact agcttgtctg agaaatttgg
    32221 ctgggctgat gttcaagttt tggaaactta ccgtggccaa gaactcaacc acatcgtaac
    32281 agaacaccca tgggatacag ctgtagaaga gttggtaatt cttggtgacc acgttacgac
    32341 tgactctggt acaggtattg tccatacagc ccctggtttt ggtgaggacg attacaatgt
    32401 tggtattgct aataatcttg aagtcgcagt gactgttgat gaacgtggta tcatgatgaa
    32461 gaatgctggt cctgaatttg aaggtcaatt ctatgaaaag gtagttccaa ctgttattga
    32521 aaaacttggt aacctccttc ttgcccaaga agaaatctct cactcatatc catttgactg
    32581 gcgtactaag aaaccaatca tctggcgtgc agttccacaa tggtttgcct cagtttctaa
    32641 attccgtcaa gaaatcttgg acgaaattga aaaagtgaaa ttccactcag aatggggtaa
    32701 agtccgtctt tacaatatga tccgtgaccg tggtgactgg gttatctctc gtcaacgtgc
    32761 ttggggtgtt ccacttccta tcttctacgc tgaagatggt acagctatca tggtagctga
    32821 aactattgaa cacgtagctc aactttttga agaatatggt tcaagcattt ggtgggaacg
    32881 tgatgccaaa gacctcttgc cagaaggatt tactcatcca ggttcaccaa acggcgagtt
    32941 caaaaaagaa actgatatca tggacgtttg gtttgactca ggttcatcat ggaatggagt
    33001 ggtggtaaac cgtcctgaat tgacttaccc agccgacctt tacctagaag gttctgacca
    33061 ataccgtggt tggtttaact catcacttat cacatctgtt gccaaccatg gcgtagcacc
    33121 ttacaaacaa atcttgtcac aaggttttgc ccttgatggt aaaggtgaga agatgtctaa
    33181 atctcttgga aatactattg ctccaagcga tgttgaaaaa caattcggtg ctgaaatctt
    33241 gcgtctctgg gtaacaagtg ttgactcaag caatgacgtg cgtatctcta tggatatctt
    33301 gagccaagtt tctgaaactt accgtaagat tcgtaacact cttcgtttct tgattgccaa
    33361 tacatctgac tttaacccag ctcaagatac agtcgcttac gatgagcttc gttcagttga
    33421 taagtacatg acgattcgct ttaaccagct tgtcaagacc attcgtgatg cctatgcaga
    33481 ctttgaattc ttgacgatct acaaggcctt ggtgaacttt atcaacgttg acttgtcagc
    33541 cttctacctt gattttgcca aagatgttgt ttacattgaa ggtgccaaat cactggaacg
    33601 ccgtcaaatg cagactgtct tctatgacat tcttgtcaaa atcaccaaac tcttgacacc
    33661 aatccttcct cacactgcgg aagaaatctg gtcatatctt gagtttgaaa cagaagactt
    33721 cgtccaattg tcagaattac cagaagttca aacttttgct aaccaagaag aaatcttgga
    33781 tacatgggca gccttcatgg actttcgtgg acaagcacaa aaagccttgg aagaagctcg
    33841 taatgcaaaa gttatcggta aatcacttga agcacacttg acagtttatc caaatgaagt
    33901 tgtgaaaact ctactcgaag cagtaaacag caatgtagca caacttttga tcgtgtctga
    33961 gttgaccatc gcagaaggac cagctccgga agctgccctt agcttcgaag atgtagcctt
    34021 cacagttgaa cgtgctactg gtgaagtatg tgaccgttgc cgtcgtatcg acccaacaac
    34081 agcagaacgc agctaccagg cagttatctg tgaccactgt gcaagcatcg tagaagaaaa
    34141 ctttgcggaa gcagtcgcag aaggatttga agagaaataa gattgaaaag tctaggcaaa
    34201 attcaatttg agaagaaaag acaactaatt ttatagtcta ttaaacgcat tgtatcacgt
    34261 ttttgaatac ctgatatgat gcgtttttta tttattttaa aaatttgcga ggtatgactt
    34321 tttatactca acaagaatca aagagaaact tagcaagcta acagtagtaa gataaaatag
    34381 gaatttgata ttagggataa gattggtaaa tagtgtaata tttttacaac aataaattta
    34441 tatagttatt tctggtttct gaaaagtatt atattttatt tcatattata caaattttta
    34501 ttttataata tcagaacata ctttttttaa aagcaaatat gatacaattt tatttgaaaa
    34561 aaataaaaaa ggagatttta ttataaaatt aaaaagactt gctttaatta gtggtatcgt
    34621 cggtcttgtg ggaggaattt tacttcttat tggtcctttt gtcttgttgg gaatagcggt
    34681 aaacacagct gctacaactc ttaatggagg agctactgca ggggcttttt caggtgtagc
    34741 cttactcttg aatgccttga agattgcaaa tcctgttctt ggtatcattg ctattgttta
    34801 ctataaagga gataagcgtg taggtgcagc tccgtctgta ctaatgattg tttctggtgg
    34861 agttagtctc attctattcc gttcttagga tgggttgggg ggatttttgc tattatcgga
    34921 ggatctctat tcctttcaac attgaagaaa ttcaaatcag aagaataaaa ggtattttag
    34981 catgaaaaga acaaaaaagt ttatcggtat aggagtagct ctattatctc tttctcttct
    35041 agttgcatgt ggaacataaa gttcaaagaa tacttcaaca agtaatgatg agaagacagt
    35101 agcaacatcc aatagttcaa aagaaacaat cactttcgat acaccggttg taacagacga
    35161 tgcgattgaa tcaatacgca cttatgcaga ttatatagat ctttataaaa atatttttga
    35221 tgattatttt actaaagctg aggaaggttt caaaggcata gctatggaaa ataatgactc
    35281 gtttactaaa ctaaaagagt caactcaaaa attattcgat gcgcagaaaa aaaggttaaa
    35341 taatgaagat agaatagaaa caaccaaaaa caatgtgatt gccaaacatt gtcaaacagt
    35401 cctttccttt ttggttttga ctagcttttt tgtgaaaaat tgtgtaaaat agaatagata
    35461 aacgagggga aacctcggaa aatttaaagg agaatccatc taatggtaaa attggttttt
    35521 gctcgccacg gtgagtctga atggaacaaa gctaaccttt tcactggttg ggctgatgtt
    35581 gatttgtctg aaaaaggtac acaacaagcg attgacgctg gtaaattgat caaagaagct
    35641 ggtatcgaat ttgaccaagc ttacacttca gtattgaaac gtgctatcaa aacaactaac
    35701 ttggctcttg aagcttctga ccaattatgg gttccagttg aaaaatcatg gcgcttgaac
    35761 gaacgtcact acggtggttt gactggtaaa aacaaagctg aagctgctga acaatttggt
    35821 gatgagcaag ttcacatctg gcgtcgttca tacgatgtat tgcctccaaa tatggaccgt
    35881 gatgatgagc actcagctca cacagaccgt cgttatgctt cacttgacga ctcagtaatc
    35941 ccagatgctg aaaacttgaa agtgactttg gaacgtgctc ttccattctg ggaagataaa
    36001 atcgctccag ctcttaaaga tggtaaaaac gtattcgtag gagctcacgg taactcaatc
    36061 cgtgcccttg taaaacacat caaaggtttg tcagatgatg agatcatgga cgtggaaatc
    36121 cctaacttcc caccattggt attcgaattc gacgaaaaat tgaacgttgt ttctgaatac
    36181 taccttggaa aataaaaaat tgtaagccta ggattgattt ctaggctttt tatgttagta
    36241 tggaagtatg ataaggaata aaaaacaaga ttatgtactg gcctacaaac aaccagcttc
    36301 aaccacttac atgggttggg aagaagaagc tttaccgata ggtaatggtt ctttaggagc
    36361 aaaagtattt ggccgtatag gggctgaacg gattcaattt aatgaaaaaa gtctctggtc
    36421 tggaggtcca cttcctgata gttcagatta tcagggtgga aatcttcagg atcagtatgt
    36481 ttttttagct gagattcggc aggctttgga gaagagagat tacaatctgg ctaaggaact
    36541 ggctgagcag cacctaattg ggccaaaaac gagtcaatat gggacctatc tgtcttttgg
    36601 ggatattcac attgagttca gccagcaagg tacgactttg tctcaggtga cggactatca
    36661 gagacagctg aatattagta aggcacttgt gacgacttct tatgtctata agggaacgcg
    36721 atttgaacgt gaagcttttg cgagttttcc agatgatctc ttggttcagc gttttactaa
    36781 ggaaggggcg gaaactctag attttactat agaactatcc ttgacctgtg atttggcttc
    36841 tgatgaaaag tatgagcagg aaaaatctga ttacaaggag tgtaagttgg atattactga
    36901 ttctcatatc ttgatgaagg gaagagttaa ggataatgat ctgcggtttg ctagttatct
    36961 agcttgggaa acggatggag atattagagt ttggtcagat agggttcaga tatcaggagc
    37021 cagttatgcc aatctcttct tggccgctaa gacggatttt gcccaaaatc ctgctagcaa
    37081 ttatcgcaag aaactagatt tagagcaaca ggtgatagac ttggtggaca cagctaaaga
    37141 aaagggctat acccaattga aatcaaggca tatcgaggac taccaagcct tattccagcg
    37201 tgttcaattg gatttggaag ctgatgttga cgcatccact acagatgatt tgttaaaaaa
    37261 ttataagcca caagaagggc aggctttgga ggagctgttc ttccagtatg gacggtattt
    37321 attgattagt tcgtccagag actgcccaga tgctctacca gctaacctac agggagtctg
    37381 gaatgcggtc gacaatcctc cttggaattc ggactatcac ttaaatgtca atctgcagat
    37441 gaattattgg ccagcctatg ttaccaatct cctagaggcg gtctttccgg tcatcaacta
    37501 tgtagatgat ttgcgtgtct atggtcgtct agcggctgta aagtatgcag gaatcgtctc
    37561 tcagaaaggt gaggaaaatg gttggttggt tcatactcaa gcgactccct ttggttggac
    37621 ggcacctggt tgggattact attggggttg gtcaccagct gccaatgcgt ggatgatgca
    37681 aaccgtttat gaagcctatt cattttatag ggaccaagac tatctcaggg agaaaattta
    37741 tcccatgttg agggaaacgg ttcgtttttg gaatgccttt ttacataagg atcagcaggc
    37801 gcagcgttgg gtgtcttctc cgtcttattc tccagaacat gggccgattt cgattggcaa
    37861 tacctatgac caatctctga tttggcagtt atttcatgat tttattcagg ctgctcagga
    37921 attgggactg gatgaggact tgttgactga ggttaaggag aagtctgatt tactaaatcc
    37981 tttgcaaatc actcaatctg gtcgaatcag ggagtggtat gaggaggaag agcagtattt
    38041 tcaaaatgag aaagtggagg cccagcaccg gcacgcttcc catctagtgg gactctatcc
    38101 tggcaatctc tttagctaca agggacaaga gtatattgaa gcggcgcgtg ctagcctcaa
    38161 tgatcgtgga gatggcggca caggctggtc caaggctaat aagatcaatc tctgggcgcg
    38221 tttgggagat ggcaatcgcg cccataaatt attggcagag cagttaaaga catccacctt
    38281 gccaaatctt tggtgtagcc atcctccttt tcagatagat ggtaattttg gtgctactag
    38341 tggcatggca gaaatgttac tccagtctca tgcagcttat ctggtacctc tagctgccct
    38401 acctgatgct tggtcaacag gttctgtttc aggcttaatg gcacgtggac attttgaagt
    38461 gagcatgagc tgggaagata aaaaactctt acagttgacc attttatcaa ggagtggagg
    38521 agatttgcga gtttcttatc cagatattga gaagagtgtg attaaaatga atcaagaaaa
    38581 aataaaagcg aaatgcatgg ggaaagattg tatttcggtg gcaacagcag aaggtgatct
    38641 tgttcaattt tatttttaag aagatgttat aaggcagtaa tttgaaactg ccttttaata
    38701 aggatttaag aatataagca gttttcaact agttgaaaaa gcgttataat gataatagga
    38761 agtaatactc aatgaaaatc aaagagcaca aacaaggaag ctagccgcag gttgctcaaa
    38821 acagtgtttt gaggttgcag atggaagctg acgtggtttg aagagagatt ttcgaggagt
    38881 ataatttgtt tgatagaggg tgggtctgat ggcttatatt gagatgaaac actgttacaa
    38941 gcgttatcag gttggggaca cggagattgt ggctaatcgt gatgtgaatt ttgagattga
    39001 aaagggggag ctggttatta tccttggtgc ttcaggtgca ggcaagtcaa cagttcttaa
    39061 ccttcttggg ggaatggata ccaatgatga aggggaaatc tggattgatg gtgttaatat
    39121 tgcggattat agttcccacc agcgcaccaa ttaccgtaga aatgatgtgg ggtttgtttt
    39181 tcagttttat aatctagttt ctaatctgac agctaaggaa aatgtggaac tggcttctga
    39241 aattgtgaca gatgccttga atcctgatca ggtcttgaca gatgtaggtc tggctcatcg
    39301 tctaaataac tttccagccc agctttctgg aggggagcaa cagcgagtct ccattgcacg
    39361 cgcggtagcc aaaaatccta aaattctcct ttgtgatgaa ccgactggag ccttggatta
    39421 tcagacgggc aagcaggttt tgaaaattct ccaagacatg tctcgtcaaa agggagcgac
    39481 ggtgatcatt gtgactcata atggagcttt ggcgcccatt gctgatcgcg tgattcatat
    39541 gcacgatgcc agtgtcaagg atgtggtgct caaccagcat cctcaggata ttgacagttt
    39601 ggagtactag catgatcaag cgaaaaactt attggaagga cttaattcag tccttcacag
    39661 gctccaaggg gcgtttttta tccatcttga tcctgatgat gttgggatct ctagccttag
    39721 taggcctcaa agtaaccagt cccaacatgg aggcgacagc taatgcttat ttaacaactg
    39781 ctcaaacctt agatttggca gtcatgtcta actatggctt ggatcaagca gaccaagaag
    39841 aactaaaaca gacggagggc gcagaggtcg agtttggcta tttgacagat gtgactatgg
    39901 ataatgggca ggatgccatt cggctgtact ccaaaccaga gcgaatttca acctttcagc
    39961 taagaaaggg acgacttcct cagtcagaca aggaaatcgc tttgaccact catttgcaag
    40021 gccaatacag cgtgggacag gagattagtt ttaaagaaaa agaagagggt cattcctctt
    40081 taaaagacca tacttatacc attactggtt ttgtggattc ggctgaaatc ctctcccagc
    40141 gagatatggg ctacgcagga agtggaagtg ggactctgac agcctatggg gtgattttac
    40201 ctagtcaatt tgatcagaaa gtctacaata tagctcgttt gaaatatcaa gatttagcag
    40261 gtttaaatgc cttttcatca gcttatgaag aaaaatccaa gcaacatcag gaagagcttg
    40321 aacaaatttt atcagataat ggcaaggtac gtctgcgact tttgaaaaaa gaaggacaag
    40381 agtctctaga caaggggcaa gagacccttg acaaggctca gactaatttg caggaaggca
    40441 agcgtcgttt agcagctgct caagctcgta tacaggctca agaaagtcaa ctagccttgt
    40501 ttcctcaagc tcagagagag caggctagtg ctcaacttac ccaagccaag caggaattgg
    40561 gcaaggaaga ggacaaacta aagcaagctg aacaaaatct agcccaagaa aaggaaaaat
    40621 tagaaaaaca tcagcaagtc ttggatgatt tggcggagcc aaggtatcag gtttataatc
    40681 gtcagaccat gccaggtggt cagggctatc ttatgtatag caatgcttca tccagtattc
    40741 gagcagtggg caatatcttt cctgtggtac tttatgccgt agcagccatg gtgaccttta
    40801 cgaccatgac tcgctttgta gacgaagagc gaactcatgc agggattttt aaggccttgg
    40861 gttatcgtag taaggatatt atcgccaagt ttctccttta tggactagta gctgggactg
    40921 tcggaacggc tctaggtagt atacttggtc attatttgct agccagtgta atttcaagtg
    40981 tcattacaaa aggcatggtg gtgggagaaa ctcagattca gttctattgg acctatagct
    41041 tactagcttt tgtcttgagc ttgttggcga gtgtgttacc agcctatctg gtggcttgga
    41101 gggaacttca tgacgaagca gcccagcttc tacttcctaa acctcctgtc aaaggagcta
    41161 aaatcttatt ggagcgtatc ggttttatct ggcgtcgtct cagttttact cataaggtaa
    41221 cagcccgcaa catctttcgt tataagcaga gaatgttgat gacaatcttt ggtgtggcag
    41281 gttctgtagc tctgctcttt gcaggtttgg gaatccaatc ttctgtagca ggagttccgt
    41341 ctaaacagtt tcaacaaatc caacagtatc agatgcttgt ctctgaaaat cctagtgcga
    41401 ccaatcagga caaggtagag ctagcagaag tgttgaaagg gcaggagata ctagcctacc
    41461 agaaaatcta ttctaaagcg ctatacaagg atttcaaagg caaagctggt cttcaaaaca
    41521 ttactcttat gatgatagag aaggaagatt tgactccctt tatccatctt caacatcatc
    41581 agcaggagct gacattaaaa gatggcatcg ttattacagc taaactcgcc cagctggcag
    41641 gtgtcaaggt tgggcagact ttagaaattg aaggtaagga actaaaggtc gttgctatta
    41701 ctgagaacta cgttggtcac tttatttata tgagtcaggc tagctatgag caactttacg
    41761 gacagctacc ccaagccaac acttatctgg tctcattaag ggataccagt gcaactagta
    41821 tcgaaagtca ggcgggcttg cttatgaatc aatctgcggt gtccagcgtt gtccaaaatg
    41881 cttcagccat tcgactcttc gactctatcg ctagctcact caatcagacc atgaccatct
    41941 tggtcatcgt atcggttcta ttagctattg tcatccttta caatctgacc aatatcaacg
    42001 tagctgagag aatccgtgaa ctctccacta tcaaggttct tggttttcat aataatgaag
    42061 tcaccctcta catttaccgt gagacgattg tgctgtccct tgtgggaatc gtacttggtc
    42121 tgatagctgg tttctattta caccaatttt tgattcaaat gatttcgcct gcgactattc
    42181 tcttttatcc gcaggtaggc tgggaagtct atgtaatccc agtggcagca gtaagcatca
    42241 ttttgacctt gcttggtttc ttcgtcaatt attatctgag aaaggttgat atgttagaag
    42301 ccctgaaatc tgtagagtaa ggtagttatt tttagctgat tgaacttcta tttactaata
    42361 ttcaaaaatc ctccgtttca aagagcaggg aactctttgt gacagaggat tttttctata
    42421 gggctttagc agctgcaatt gcggcttcga agtttggctc agaattgata ttatccacgt
    42481 attcaacgta gcgaatcgta ttgtcagtat cgaggacaaa gactgcgcgt gctaataggt
    42541 gccattcgtt gatcaagagg gcataatcgc gcccgaaaga atggtcaaag tagtctgaaa
    42601 gcataatggc attgtcaagg ccttcagcac cgcaccaacg tttttgagca aaaggtaggt
    42661 ccattgaaac agtcaatacg accgtgttgt ccagtccagc caattcttca ttaaaacgac
    42721 gtgtttgagt tgagcagatg cctgtatcga tagaaggaac gacactcaag acttttttct
    42781 tgccatcaaa atcagccaga gattttttag aaagatctgt tgtagtaaga gaaaaatcaa
    42841 gcgccttgtc gccgacttgt agttgtttac ctgtaaagct cacaggattt ccgagaaaag
    42901 ttaccatagg atactccaat cttttttctt ccattttagc tgaaacagtc ggaattttcc
    42961 aatgatttga ccggaaatat gggcatagaa aaaacgccag ctcatgtgag aatgacgttt
    43021 ttcataggtt tattttgcca atccttcagc aatcttgtca aggttgtatt tcatcatgct
    43081 gtagtagctg tcgccttctt taccttgttc tgcgatagag tcagtaaaga tttgagcgta
    43141 gattgggatg tttgtgtctt gagaaacagt tttcattgga cggtcatcca cacttgattc
    43201 tacaaagagt gatggaactt ttgtttggcg aagtttttca accaaggtct tgatttgttc
    43261 aggagttcct tcttcttcag tattgatttc ccagatgtaa gcacttggga caccataggc
    43321 tttagagaag tatttgaatg ctccttcgct ggttacaatg agtttctttt cagcagggat
    43381 cttattaaat ttatccttac tttctttatc aagtttgtct aacttatcag tatattcttt
    43441 gagatttttt tcatagaatt ctttattgtt agggtctttg gcgctcaatt gtttggcgat
    43501 atttttagca aaaataatac cgttttcaag gttaagccaa gcgtgtgggt cttcttttcc
    43561 tttttcattt tgaccttcaa ggtagataac atcaacgccg tcgctgactg cgaagtagtc
    43621 tttgttttca gttttcttgg cattttctac caattttgta aaccaagcat tgccacctgt
    43681 ttcaaggttg ataccgttat agaaaatcaa attagcctca gaagttttct taacgtcttc
    43741 aggaagtggt tcgtattcgt gtgggtcttg cccaatcgga acgatactat gaaggtcaat
    43801 tttgtcacca gcaatatttt tagtaatatc agcgatgatt gagtttgtag caacaacttt
    43861 tagtttttga ccagaagttg tatctttttt tccgctagca catgctacaa gaatgattgc
    43921 agaaagaaag agaacgagta atgtacctaa ttttttcatt agatcctcca atttattagg
    43981 gctttgcccc ttattttaac aaatgtttat ttttcagttt caaatatcgt tgtttgggag
    44041 cgataaagaa gctaatgaga aagaaactag cagctgtaag cacgatacta gaacctgccg
    44101 caacattaaa actatagcca ataaagagtc ccaaaactga agcagtagct ccgaaggttg
    44161 aggaaaggaa aatcatactt ttcagactat tagcatacag ataagcagtt gcagctgggg
    44221 taatcagcat ggctacaatc aggatagttc cgacactttg catggctgtc acagacacga
    44281 gagtcaggag taccatgaga aggtagtgat agaaattgac aggcattccc atggctttag
    44341 ccaagagttc atcaaaggaa gttatcaaga gttgcttgaa gaaaatccag attaacaaga
    44401 ggatagctgc ccccacaccc atagtaataa acatatccgt atcttggacg gccaggatat
    44461 taccaaaaag gatatggaaa aggtcagttg aacttttagc gacaccaatc aagatgatac
    44521 cgagggctaa gaaagaagaa aaggtaatgc cgatggcggt atcgcttttg ataatcgagt
    44581 ttcctttgat gtaggtaatg atgatggcag ctagcaatcc aaagacaatg gctccgataa
    44641 agaagtcaag gcccaagatg aaggataggg ctacacctgg taagacagca tgtgaaatgg
    44701 catctcccat gagtgacatc ccgcgtagaa taatgaaaca tcccacagct ccagctacaa
    44761 tcccgacgac aatagctgtt atcaaggcat tttgtaggaa atggaatttt tgcaatccat
    44821 cgataaattc tgcaatcata ggtcacctcc attgaaaaag agttgattac cgtaagcttc
    44881 ttttagattg gtttcggtaa aagtttcttt tgttggacca aaggcaatca cttctcgatt
    44941 gacaagtaag acttgatcga agtagtgggg aatcttgctg aggtcgtggt gaacgatgag
    45001 aaccgtcttc ccagcttttt tcaaatctct cagcgtattc atgatgattt cctcactgac
    45061 agagtcaatc ccagcaaagg gttcatccaa gaggatatag tcggcttcct gcaccaaaca
    45121 tctggcaatc aagacccgct ggaattgacc tccagacagt tgactaattt gacgttcagc
    45181 gtagtcagct aggccgacga tttcaagggc ctcttgcact ttcttccaat gtttagcctt
    45241 taaacttcga aagagaggaa tagagggaaa tagtcctaac gagacgcatt ccttgacctt
    45301 gatgggaaag ttgtagtcga tattgatttt ttgttcgaca taggcaattc ggtgtaagga
    45361 ttttttaact tccttgtcat cgagaaatgc ctgaccttga tgtgggataa ttcccaacat
    45421 accttttaat agtgttgatt tcccagcgcc gtttggacca atgatgccgg taattgttgg
    45481 tccatggagc actagtgaaa tatccttaag tgccaacgtt tctttgtagg agacactgag
    45541 gttttcgata cgtatcataa acttgtattc ctcctgtctc ttaatataca ttaa
//
LOCUS       DAVKJJ010000010        44107 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000010 DAVKJJ010000000
VERSION     DAVKJJ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44107
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.010"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            126..2018
                     /gene="pepO"
                     /locus_tag="VW144_000551"
     CDS             126..2018
                     /gene="pepO"
                     /locus_tag="VW144_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEU9600037.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(2140..2769)
                     /locus_tag="VW144_000552"
     CDS             complement(2140..2769)
                     /locus_tag="VW144_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9600038.1"
                     /translation="MKIYKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            3763..5985
                     /locus_tag="VW144_000553"
     CDS             3763..5985
                     /locus_tag="VW144_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU9600039.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            6015..6458
                     /locus_tag="VW144_000554"
     CDS             6015..6458
                     /locus_tag="VW144_000554"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEU9600040.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(6725..6910)
                     /locus_tag="VW144_000555"
     CDS             complement(6725..6910)
                     /locus_tag="VW144_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600041.1"
                     /translation="MKKIIFIKTIQLLVIDGIMLAFLTFKRGLTWDWILIYSGWLIFF
                     HPVLLTYLSNQLCDHFS"
     gene            complement(6929..7342)
                     /locus_tag="VW144_000556"
     CDS             complement(6929..7342)
                     /locus_tag="VW144_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600042.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFIIRLETSFEWLLLSGLIFFLA
                     QGLLLFLLIVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMS
                     IITACHSTFYYMTADYLRENYPDFYDKHISLWECL"
     gene            complement(7473..8180)
                     /locus_tag="VW144_000557"
     CDS             complement(7473..8180)
                     /locus_tag="VW144_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEU9600043.1"
                     /translation="MKKSLKIFATSKWFDLFGVALVVGIAIASGYLNSRLDKFVDWGL
                     WTALVPFGLISVTNVGISMLSTRFTGKLSKWGNYFGIVNTILSGAIDYILGNKAAIIT
                     YPVTFLIYTFAIKKWKASQEGRPNQISQKQVKLAVIIISIIAFLFAFVTNYIGYEGKM
                     NLLAYVTTIAFALSLIANALNALKLTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFY
                     ILNAIGALFVWNDEEVR"
     gene            complement(8299..8949)
                     /locus_tag="VW144_000558"
     CDS             complement(8299..8949)
                     /locus_tag="VW144_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU9600044.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMVIAKQLYVEKIN"
     gene            9061..9903
                     /locus_tag="VW144_000559"
     CDS             9061..9903
                     /locus_tag="VW144_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU9600045.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQLHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            9979..10416
                     /locus_tag="VW144_000560"
     CDS             9979..10416
                     /locus_tag="VW144_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU9600046.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            10485..10787
                     /locus_tag="VW144_000561"
     CDS             10485..10787
                     /locus_tag="VW144_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600047.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSFISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(10878..11951)
                     /locus_tag="VW144_000562"
     CDS             complement(10878..11951)
                     /locus_tag="VW144_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEU9600048.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPSQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSRQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILDAGISSINDLASLKALEYIHHLFVQAQSLTPK
                     ERETLGRLTQLLIDTRYHAWKNR"
     gene            12107..12784
                     /locus_tag="VW144_000563"
     CDS             12107..12784
                     /locus_tag="VW144_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9600049.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            12777..13751
                     /locus_tag="VW144_000564"
     CDS             12777..13751
                     /locus_tag="VW144_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9600050.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(13800..15743)
                     /gene="thrS"
                     /locus_tag="VW144_000565"
     CDS             complement(13800..15743)
                     /gene="thrS"
                     /locus_tag="VW144_000565"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU9600051.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(15903..16124)
                     /locus_tag="VW144_000566"
     CDS             complement(15903..16124)
                     /locus_tag="VW144_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU9600052.1"
                     /translation="MELQELVERSWAIRQAYNELEVKHHDSKWTVEEDLLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIW"
     gene            16981..17610
                     /locus_tag="VW144_000567"
     CDS             16981..17610
                     /locus_tag="VW144_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU9600053.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTPDFPEEFKPLAKAQADEFDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNGKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            17751..18020
                     /gene="rpsO"
                     /locus_tag="VW144_000568"
     CDS             17751..18020
                     /gene="rpsO"
                     /locus_tag="VW144_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU9600054.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            18835..19449
                     /locus_tag="VW144_000569"
     CDS             18835..19449
                     /locus_tag="VW144_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEU9600055.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(19742..20491)
                     /locus_tag="VW144_000570"
     CDS             complement(19742..20491)
                     /locus_tag="VW144_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU9600056.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            20624..22960
                     /locus_tag="VW144_000571"
     CDS             20624..22960
                     /locus_tag="VW144_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU9600057.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFQGQVVYPMLSNLPFSSDRKWGAMELEGLGIVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLNY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELIALAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRRSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEIATLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            23062..23739
                     /locus_tag="VW144_000572"
     CDS             23062..23739
                     /locus_tag="VW144_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEU9600058.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVPHVSK"
     gene            23726..24523
                     /locus_tag="VW144_000573"
     CDS             23726..24523
                     /locus_tag="VW144_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU9600059.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            24540..25643
                     /locus_tag="VW144_000574"
     CDS             24540..25643
                     /locus_tag="VW144_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU9600060.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTPLASGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPDLTGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            25650..25796
                     /locus_tag="VW144_000575"
     CDS             25650..25796
                     /locus_tag="VW144_000575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600061.1"
                     /translation="MKIKEQTRKLAAGYSKHCFEVVDRTDEVSNHTHGKATLTWFELD
                     FRRV"
     gene            25899..26918
                     /gene="galE"
                     /locus_tag="VW144_000576"
     CDS             25899..26918
                     /gene="galE"
                     /locus_tag="VW144_000576"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9600062.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKVMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            26920..27885
                     /locus_tag="VW144_000577"
     CDS             26920..27885
                     /locus_tag="VW144_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9600063.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAAHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(27936..28142)
                     /locus_tag="VW144_000578"
     CDS             complement(27936..28142)
                     /locus_tag="VW144_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU9600064.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            28194..28670
                     /locus_tag="VW144_000579"
     CDS             28194..28670
                     /locus_tag="VW144_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEU9600065.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            28679..29350
                     /locus_tag="VW144_000580"
     CDS             28679..29350
                     /locus_tag="VW144_000580"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU9600066.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            29481..29819
                     /locus_tag="VW144_000581"
     CDS             29481..29819
                     /locus_tag="VW144_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEU9600067.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            29822..30451
                     /locus_tag="VW144_000582"
     CDS             29822..30451
                     /locus_tag="VW144_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEU9600068.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            30691..31842
                     /locus_tag="VW144_000583"
     CDS             30691..31842
                     /locus_tag="VW144_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9600069.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            32155..32904
                     /gene="truA"
                     /locus_tag="VW144_000584"
     CDS             32155..32904
                     /gene="truA"
                     /locus_tag="VW144_000584"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU9600070.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFILDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            32894..33667
                     /locus_tag="VW144_000585"
     CDS             32894..33667
                     /locus_tag="VW144_000585"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9600071.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            33645..34106
                     /locus_tag="VW144_000586"
     CDS             33645..34106
                     /locus_tag="VW144_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9600072.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            34331..35668
                     /locus_tag="VW144_000587"
                     /pseudo
     CDS             34331..35668
                     /locus_tag="VW144_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(35796..35999)
                     /locus_tag="VW144_000588"
     CDS             complement(35796..35999)
                     /locus_tag="VW144_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9600073.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(35992..37074)
                     /locus_tag="VW144_000589"
     CDS             complement(35992..37074)
                     /locus_tag="VW144_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9600074.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTIFETAEFENLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     IIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(37200..37982)
                     /locus_tag="VW144_000590"
     CDS             complement(37200..37982)
                     /locus_tag="VW144_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEU9600075.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(37982..39325)
                     /locus_tag="VW144_000591"
     CDS             complement(37982..39325)
                     /locus_tag="VW144_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU9600076.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(39424..40740)
                     /locus_tag="VW144_000592"
     CDS             complement(39424..40740)
                     /locus_tag="VW144_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEU9600077.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYTTVAGTGVNIFDAEAHFLS
                     NKVIEIQAGDEKQELTAETIVINTGAVSNVLPIPGLATSKNVFDSTGIQSLDKLPEKL
                     GVLGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            40934..42160
                     /locus_tag="VW144_000593"
     CDS             40934..42160
                     /locus_tag="VW144_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEU9600078.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPRVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSASGKEKTDSLTQGMTANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGILGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            42359..43933
                     /locus_tag="VW144_000594"
     CDS             42359..43933
                     /locus_tag="VW144_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9600079.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
BASE COUNT        12830 a         8296 c         9990 g        12991 t
ORIGIN      
        1 ttatgcgttt tatcatggaa agatttttta taatagctaa aaaataatag ttaaatggtt
       61 attaattgca ttccctagtg atttttgtta agataaatgc aaatacaaat gaaagcgaga
      121 acaagatgac acgttatcaa gatgattttt atgatgctat caatggagaa tggcaacaga
      181 cagctgaaat cccagcagat aagtctcaaa ctggaggttt tgttgattta gaccaggaaa
      241 ttgaagacct gatgctggcg acaacagaca agtggttagc aggtgaagag gtgcctgagg
      301 atgctatctt ggaaaacttt gtcaaatacc accgcctagt tcgtgatttt gacaagagag
      361 aagctgacgg tatcacacct gtcttaccac tccttaaaga attccaagaa ttggaaactt
      421 ttgcggattt tacagctaaa ctagcagagt ttgagcttgc aggaaaacca aacttccttc
      481 cttttggtgt atcgccagac tttatggatg ctagaatcaa tgttctatgg gctagcgctc
      541 caagcacaat cttgccagat acgacctact atgcagaaga acatcctcag cgcgaagaac
      601 tcttgactct ttggaaagaa agcagcgcaa atctcctcaa ggcttatgat ttctctgatg
      661 aagaaattga agacttgcta gaaaaaagac ttgaattgga ccgccgagtt gcggcagtgg
      721 tgctctctaa tgaagaaagt tcagaatatg ctaaactcta tcatccatat tcttacgaag
      781 atttcaagaa attcgcgcct gccctacctt tggatgactt cttcaaagca gttattgggc
      841 aattaccaga caaggttatt gtagacgagg aacgtttctg gcaagcagca gagcaattct
      901 acagtgagga agcctggtct ctccttaaag caaccttgat tttgagtgtt gtcaatcttt
      961 caaccagcta tttaacagag gatatccgtg ttttgtctgg tgcctacagc cgtgcccttt
     1021 ctggagttcc agaggcaaaa gataaggtca aagcagctta tcatctagca caagaacctt
     1081 tcaagcaagc cctgggtctt tggtacgccc gtgagaagtt ctctccagaa gccaaggcgg
     1141 atgtggagaa aaaagtggca accatgattg atgtttataa ggagcgtctg cttaagaatg
     1201 actggctcac tccagaaacc tgtaaacagg ctatcgtgaa gctcaatgtg atcaaacctt
     1261 atattggcta tccagaagaa ttgcctgcac gttacaagga taaggtagtg aatgaaactg
     1321 ccagtctttt tgagaatgct ctagcctttg cgcgtgtgga aatcaagcac agttggagta
     1381 agtggaacca gcctgtagat tataaggaat ggggcatgcc tgctcatatg gtcaatgcct
     1441 actacaatcc tcagaagaac ctgattgtct ttccagcggc cattttacag gcgcctttct
     1501 atgacttgca tcagtcatct tctgctaact acggtggtat tggggcagtg attgcccatg
     1561 aaatttccca cgcctttgat actaacgggg cttcctttga cgaaaatggt agcctcaagg
     1621 attggtggac agagagcgac tatgctgcct tcaaggagaa aacacaaaaa gtcattgacc
     1681 aatttgatgg acaggattct tatggagcaa ccattaacgg taaattgact gtatcagaaa
     1741 acgtggctga cttgggagga atcgcagcag cgcttgaagc agctaagaga gaagcagact
     1801 tctcagcaga agagttcttc tacaacttcg gtcgcatctg gcgcatgaaa ggtcgaccag
     1861 aatttatgaa acttttggct agcgtcgatg tgcacgcacc agccaaactc cgtgtcaatg
     1921 tgcaagtacc aaacttcgac gatttcttta caacctatga tgtcaaagaa ggagacggaa
     1981 tgtggcgttc accagaggag cgcgtgatta tttggtaata caaaaaatct agtcatcaga
     2041 aaaaggcatc caatcacgtg tgaaaacctt gataggatgc cttttgtaat agaaagattt
     2101 gctggatagt ttacttaaat ttcgattgtc atcatcttgc taaaaaaagg gattgaaggc
     2161 cttttcgtga gcgatagtag tagctggacc atgtcctgga tagacatcgt agtttggtag
     2221 ggtgaagagt tgggtctgga tactatgaag gagttgctcc atgctaccag tcggaaggtc
     2281 ggtccgtccg atagtttcgc ggaatagagc atctcccgtc aagactaggt gagcatcagg
     2341 aaagactagg gaaacaccac cgatagagtg ccctggggtc ggtagaacct taaaacgaaa
     2401 ttcctctagt tggtattctt cgtgaaagac aaaggtgtgt tctgcaggtt ttgtgaccac
     2461 atctgccata tcatcgtggc gagggagacc ggagagatta tcgacaggag tgtagagcca
     2521 gctggcttcg ctctctgcga tatagacagg aggattgcca aacgtctcgc gaaccaagtc
     2581 cagactcatg atatggtcat aatgggcgtg ggtcaagaga atagcacaga tcggtttgtt
     2641 gatcttctcg attgtctgac gaatggcttc ccaatgacta ccaggatcga cgacgatgag
     2701 gtgcttttcg ccttctagat aataggtatt ttcataggca acaggattca cggttttata
     2761 gattttcata ctagctccaa tctcaaagaa tatactaata ctcttcgaaa atctcttcaa
     2821 accgcgtcaa ctttatctgc aacctcaaag cagtgctttg aacagcctgc ggctagcttt
     2881 ctagtttgct ctttgatttt cattgagtat aaattaagta tagcacagtt agggagaata
     2941 ggtaaggatt taaaatgaac ttaaaaacca gcatgactag atgaagacag gctggttatc
     3001 aatttatcaa tattggaaag gtgtcaattg cccttcatag gttgcataaa ctccgtcatt
     3061 agcttgtttg atagacgtga tgtttagagt gctaccgtag ttggcttctc atttgtcgct
     3121 atattcacta taagttatcc tactgggcag gttctggtcg ttagcatagt attgaacgag
     3181 ttttttatag ctttgctggc tgaataggaa caggcagcaa actcctaaca ttaggagagc
     3241 aaagatagaa atagcagttt ttttcatagg tattctcctt ataaattaaa ggatagaaga
     3301 aagtatcttg aggattttta tactcttcaa aaatctcttc aaaccgcgtc aacgtcgcct
     3361 tgccgtatat atgtgactga ctttgtcagt cttatctaca acctcaaagc agtgctttga
     3421 gcaacctgcg gctagtttcc tagtttgctc tttgattttc attgagtatt aattccaaaa
     3481 tccagtcaag gtaccagtgt atgtgatacg agttccaccg tttatctttc gtatcttcaa
     3541 tggaattggg gaaatcagaa agtctttggg ttacaatatg gtttccgttc ttctatctct
     3601 ccttctatca cctaagttta aaatctaact tcgtagtata ttatgggcca ataattcaga
     3661 attataaagc aattttgaat tatttttttc ttattctgtt ccaatctagt tttcatattc
     3721 ttaaaaaagt gataaaatgg taggaagaat tggagagtag agatgccgaa agaagtgaat
     3781 ttaacaggcg aagaagttgt cgctttaacc aaagaatatt taacggaaga ggatgttcat
     3841 tttgtccata aggccttggt ctatgctgtt gaatgccaca gtggtcaata tcgcaaatca
     3901 ggcgagcctt atatcattca ccctatccaa gtggcaggta ttttagctaa gctaaagctg
     3961 gatgctgtaa cagtagcttg tggattcttg catgatgtgg tggaagatac agatgcgacc
     4021 ttggacgatt tggaaagaga gtttggtcct gatgtgcggg tgattgttga cggagttacc
     4081 aagcttggca aggtcgagta caaatcgatc gaggagcaat tagcggaaaa tcatcgcaag
     4141 atgctcatgg ccatgtctga ggacatccgc gttattttgg tcaaactgtc tgaccgcttg
     4201 cacaatatgc ggaccctgaa acatcttcga aaagacaagc aggagcgtat ttccaaagaa
     4261 accatggaaa tctatgcccc acttgcccat cgtttgggga tttccagtgt caaatgggaa
     4321 ttagaagact tgtctttccg ttatctcaat ccaacggagt tttacaagat tacccatatg
     4381 atgaaggaaa agcgcaggga gcgtgaggcc ttggtggatg aggtagtcac aaaattagag
     4441 gagtatacga cagaacgtca cttgaaaggg aagatttatg gtcgtcccaa gcatatttac
     4501 tcaattttcc gcaaaatgca ggacaagaga aaacggtttg aggaaatcta tgatctgatt
     4561 gctattcgtt gtattttaga tacccaaagt gatgtttatg ccatgcttgg ttacgtgcat
     4621 gaattttgga aaccgatgcc aggtcgcttc aaagattata ttgccaaccg caaggccaat
     4681 ggttatcagt ctatccatac gactgtttat ggaccaaaag ggccgattga attccagatt
     4741 cgaaccaagg aaatgcacga ggtggctgag tacggggttg cggctcactg ggcttataag
     4801 aaaggtataa aggggcaagt taacagcaag gaatcagcta ttggaatgaa ctggatcaag
     4861 gagatgatgg agctccaaga ccaggctgat gatgctaagg aatttgtgga ctctgttaag
     4921 gaaaactatt tggctgagga gatttacgtt tttaccccag atggagctgt ccgttccctt
     4981 cccaaagatt caggaccgat tgattttgcc tacgaaatcc ataccaaggt cggtgaaaaa
     5041 gcaactggtg ccaaggtcaa tggccgcatg gttccactga caaccaagtt aaagacaggg
     5101 gatcaggttg aaattatcgc caacccgaac tcctttggac ctagccgtga ctggctcaat
     5161 atggtcaaga ctagcaaggc gcgcaataag attcgccagt tctttaaaaa ccaagataag
     5221 gaattgtctg tcaacaaggg tcgtgagatg ctgatggctc agttccaaga aaatggctat
     5281 gtggcaaata aatttatgga caagcgccac atggatcaag ttctgcaaaa gaccagttac
     5341 aagacagaag actccctctt tgcggccatt ggttttgggg aaatcggtgc gattaccgtc
     5401 tttaatcgtc tgactgaaaa ggaacgccgt gaggaagagc gtgccaaggc caaggctgag
     5461 gcagaggagc ttgtcaaagg tggcgaggtc aaggttgaaa ataaagaaac tctcaaggtc
     5521 aagcatgagg ggggagtggt tattgaaggt gcttctggtc tcctagtgcg gattgctaag
     5581 tgttgtaacc ccgtgcctgg tgacgatatt gttggctaca ttaccaaggg tcgtggtgtg
     5641 gctattcacc gtgtggactg tatgaacctg cgtgcccaag aaaactacga gcaacgtctc
     5701 cttgatgtgg aatgggaaga ccagtactct agctcaaata aggagtatat ggcccatatc
     5761 gatatctacg gtctcaaccg tacaggactg ttgaacgatg tactgcaagt tctttcaaat
     5821 acaaccaaga atatttcaac ggtcaatgcc caaccaacca aggatatgaa gtttgctaat
     5881 atccatgtgt ccttcggtat tgccaacctc tctacactga ccacggttgt cgataaaatt
     5941 aagagtgtgc cagaagttta ctctgtcaaa cggaccaacg gctagttgtc ctagctctta
     6001 ctagaaaggc tattatgaaa atcattatcc aacgggttaa aaaagcccaa gtgagtatag
     6061 aaggccagat tcagggaaaa atcaatcagg gacttttatt gctggttggt gttggaccag
     6121 aggaccaaga ggaagatttg gactatgctg tgagaaaact ggtcaatatg cggatttttt
     6181 cagacgcaga aggcaagatg aacctgtctg tcaaagatat tgaaggagaa atcctctcta
     6241 tttctcagtt taccctcttt gcggatacta agaaaggcaa tcgtccagcc tttacagggg
     6301 cagctaaacc tgatatggca tcagacttct atgatgcttt caatcaaaaa ttagcgcaag
     6361 aagtgcccgt tcagacaggt atctttggag cagatatgca ggttgagctg gttaataacg
     6421 gacctgttac cattatccta gatactaaaa agagataaga aagaccaagc ccagtcggct
     6481 tggtctttct catctatcat aaaatactcc aaaaagaaat cggttcttga tatgcttggg
     6541 ggaagtctct tttcaggctt tggcagatgc gataggaagg gatgagatgt cctaggatga
     6601 ggagagttcc ctgaaggaaa agtggaatat cacttaaaga caccaaggag agaatcatca
     6661 ggctatccca taggagaatt tgtaaagcaa aacgccagaa tctcggtcta atctgggaat
     6721 agagttaact aaagtggtca caaagttggt ttgaaagata ggtcaatagc acaggatgaa
     6781 agaaaatgag ccaaccgcta taaatcaaaa tccagtccca agtaagcccc cttttaaatg
     6841 tcaaaaatgc cagcatgatt ccatcaatga caaggagttg aatggttttg atgaagatga
     6901 tttttttcat gctaaaacct ccttttcctt agagacactc ccataaagag atgtgtttgt
     6961 cgtaaaagtc tggatagttt tctcttaggt agtcagctgt catataataa aaagtagaat
     7021 ggcaagcagt gataattgac ataagagatg aaaaacgctg agaactaatg aaggctaaga
     7081 gaacaaaaaa tagaaaatca atggtgagaa cccctaaata gtagaagcga atctttttat
     7141 tgatttgagg ataaatctca gcgaattgat gtttgagtcg gacgatcaag agaaatagca
     7201 ggagtccttg agcaaggaag aaaattaaac cagagagcaa gagccactca aaagatgtct
     7261 cgagtctaat gataaaaaat gcaaatagta gcaaatcaat aactgcaatt gctataaaat
     7321 ggattctata gagttttttc atgacaagac ctccctcttt tatctaactt cattctactc
     7381 caaaagaatg ggagttacaa ctaaaatgat aaaaatagca gaagggagat tctcttaagt
     7441 tggctagtat tctttatttg agtttccttc tattatctaa cttcttcatc attccagaca
     7501 aataaagctc cgattgcatt gaggatataa aagatgtatt taccgatatt ggcgaagttt
     7561 ccttgaatac cagcttttgt cagctgaacg aaattgtaaa tcaaccaaaa gccccactga
     7621 gttgttagtt ttaatgcatt caaagcattg gcaatgaggg atagtgcaaa ggcaatagtt
     7681 gttacgtagg caaggagatt catcttgccc tcatatccga tatagttggt cacaaaggca
     7741 aagaggaagg cgatgatgga aatgatgatg accgccaatt ttacctgttt ttggcttatt
     7801 tggttgggtc tgccttcttg cgaagctttc catttcttta tagcaaaggt ataaatgagg
     7861 aaggtgacgg gataggtaat gatggccgcc ttatttccaa ggatataatc aatagcaccg
     7921 gacaaaatgg tattaacaat accaaagtaa tttccccatt tgcttaattt ccccgtgaaa
     7981 cgagtggaca acatggaaat cccaacgttg gttacggaaa tcaatccaaa gggtacaaga
     8041 gctgtccata gtccccagtc tacaaattta tcgaggcgtg agttgagata accagatgca
     8101 atcgcaatcc caacgaccaa agcaaccccg aagaggtcaa accatttaga tgtagcaaaa
     8161 atttttagtg attttttcat aggttaaact acctttcttt ttttttcaaa tattctacca
     8221 ccaaatgaaa gtaaaataaa atgatagaaa taaaaccctg aaaataaaac tttcagggtt
     8281 ggtaggggac ttgagaaatt agttgatttt ctcgacatag agttgttttg caatgaccat
     8341 attcacttgt aaatcctcgt cgttactgag gatagtgaag gtattgctga agccatcaaa
     8401 ctgcttgact tggagctggt caccgatgtg aagtgagtgc ttgtccagat aatggagaat
     8461 gtcaaaacta tcgtgcaccc gagtcaggcg gtaggcgcca gcttccttga tatcagctag
     8521 tgggaggtta ttgatttcaa cgaggagttc tcccttggca ggaatagttc ctccgtgggg
     8581 gcaggtttta gggaaaccta gcagtttatc tagtctttcc acgaacaggt cagagacagt
     8641 gtgttccaag acctcagctt cctcgtgaat ctggtcactt gtatagtcta aatgatgaac
     8701 tagaaaaact tcaatcaagc ggtgcttacg atagagctca gagaccagtt tgagaccgag
     8761 gtcagtcagt agatagccac attccttgtc ctttaggatg agattttcac ttttcattcg
     8821 tttgatcatt tcagttacgg cagggggaga gacttgcatg cgagccgcaa tttccttgtt
     8881 ggtaatctta tgcaggtcta tgccaatttc ataaatacat tttagatagt cttctttgtt
     8941 tggggtcatt cgtttcctct tgtctaatat ctttatctag tatatcaaaa aaagctagat
     9001 ttctctagct gtgacttttt atgttatact tattgcaaga gaaaaaacga ggagatggat
     9061 atgacgaaaa tagctcttct ttcagatatt catggaaata ccaccgcctt ggaggctgtt
     9121 ttggcagatg ctcggcagct aggagtggat gaatactggc ttttgggaga tattctcatg
     9181 ccagggacag gacgtagaag gattttggac ttgttggatc aactaccgat tacggctaga
     9241 gttttgggaa actgggaaga cagtctttgg catggtgtcc gtaaggaatt ggatagtact
     9301 cgccccagtc aacgctatct cttgcgccag tgccagtatg ttttagagga aatttcccta
     9361 gaagaaattg aagtgctcca caatcaacct cttcaacttc atcgtcagtt tggggatttg
     9421 acggtgggaa ttagccatca tctgcctgat aagaactggg ggcgagagtt gattcatact
     9481 ggcaaacaag aggagtttga ccgcttggtg actcatcctc cctgtgatat tgctgtttat
     9541 ggacatattc accagcagtt gcttcgttac gggactggtg ggcaattgat tgtcaatccg
     9601 ggttcgattg gccaaccttt ctttttggac gctcagctac ggaaggactt gcgggcccag
     9661 tatatgattt tggagtttga tgacaagggc ctggtagata tggacttccg acgggtagac
     9721 tacgatgtgg cagctgaatt gcagctggct aaagacctga gacttcccta ttttgaggtt
     9781 tattatgaaa gtctggtcaa tgggatccac catactcatc atcaggaatt tttgagagaa
     9841 ttggctcaga aagagggctg cgatcgggag ttagacgact ggttgaaaag tggtaacgat
     9901 tgacattaca actaaaaagg gtataataat agaaaaagaa gagtagaggc agggtagcct
     9961 cgtaaaaagg agaagaggat gcaaattcca agtagattta ccattgcgac tcatatgctg
    10021 ataatcattg ccctcgaggg gaaggaaagc aaggtgacca gtgattttct ggctgctagt
    10081 gtcggggtca atcctgtcat tatcagaaag atcttgtccc agttgaagaa ggcagagctg
    10141 atttcagtag cgcgtggaac gggcggaaca gagattgtca aggaccttaa ggatattagt
    10201 cttttagatg tttatcaggc ggtcgaatgt cttggtaaga caggtcaact cttcagtttc
    10261 catgacaatc cgaatcccaa ttgccctgta ggagctcata ttcatgatgt tttggatcaa
    10321 aaattggaga gaattcagtt gactatggag gcagaacttg gtcagaccag tctagaaaaa
    10381 gtcgtggccg atgcagagag tcagatgaag gattaagagg gataaatgcc ctcttttttt
    10441 ctttggataa ttatgataaa atgtagctat taaaggagta agaaatgtat cttgttatag
    10501 gaattgtatt agcctttata gtgtcatttt ggaaggataa tagaagtttg tggaatccag
    10561 tattattttt attatccttc atttcaagtt atttttatct atcctacctt ttttataaaa
    10621 atggatacga gaatgttcag ttagcttttt atgtatttgc ctttgtctta cttccttttt
    10681 tgctttttct aagtggcatc tttttaatct ataatggggt cattttgttg aaacgagagg
    10741 gacgttccaa gccccattat ctctcaatgc tctttgattt ttattgacta ttagattcta
    10801 tttaaaaaga cttagaatgc acatgaaagt atgtattcta ggtcttttgt tttgtataca
    10861 tttaattttc aagagtatta tctatttttc catgcatggt aacgagtatc aatcaataac
    10921 tgggtaaggc gacccaaggt ttctctttct tttggagtga gggattgagc ttggacaaag
    10981 agatgatgaa tgtattcaag cgcctttaag gaagccaagt cattgataga gctaatacca
    11041 gcatcaagaa tagtgccaaa gaagaggtcg aagtagagct gaagttcttc gtcagggact
    11101 gtggccaccc attctttaaa ggttgtgtct acttgccggc tatcactgtt ggtcttatcc
    11161 agttgaacga agtgcttgtc ctcaatctgc caactaaagg tatcgtgctg ggcgatgcca
    11221 cccagggcag tactctgaac gatgatttgg tgagcaggaa tttccagcat cataccgata
    11281 atggaacctt gtggaatgaa tatcttgctt ctatccatta tcctttgata acccgcagtc
    11341 tgtgtcaatt cttgatggag accaggtgca tcaaatgtat aaactgctgt gatctgattt
    11401 tgcaaacttt gctcaatttg gctagcagca tagatagcga gatttcctcc cttggaatgc
    11461 ccagccagaa taaccttttg cttaggatga tgggcaaaaa agttctttaa atagcgaagg
    11521 gcgtgctttt gagaaggaat ttccttcata taggtcaggt ggaaatcttc cttccagcca
    11581 atgatactgt catctgtccc acgaaagaca atcagatagg tatcgaggct gacacgataa
    11641 gtcatagccg caaattgctt ttgcagttca gggtcgatgt cgttgataaa atgggagagt
    11701 ttgcaatttt tgaagcgctt gtgttgagcc aattcatcta ataattgaag gcgatttttg
    11761 ctagtaagca tggtgggctc tcttggaacc tgaggtgcta gatctaaaag acgttgagga
    11821 agtgtggaga ccagattatc aaaggagagg taggtgattt ctattaaggt tagaatgtct
    11881 aactcattca agggaaggtc gtaataagaa tcatatgcga catctttcag atagtcaaaa
    11941 atattggcca taagagctcc tttcttttta tttatcttag caaatttccc tcaaattagc
    12001 tagtagcata gcctgtttgt gctggctaaa aacaggctat ttcaaattca gtttcagacc
    12061 atctagcatg gaaaaatctg ttataataat ggaaaaggag aagcgcatgc acaagatttt
    12121 attaatagaa gatgatcagg tcattcgtca acagattggg aaaatgctct ctgaatgggg
    12181 atttgaagtg gtcctggtag aagactttat ggaagttttg agtctatttg ttcagtcgga
    12241 acctcatctg gtcctcatgg atattggttt gcccttgttt aatggttatc actggtgtca
    12301 ggaaatccgc aagatttcca aggtacctat catgtttctt tcttcgagag accaggctat
    12361 ggatattgtc atggcaatca atatgggggc ggatgacttt gtgaccaagc cttttgacca
    12421 gcaggttctt ttagctaagg ttcagggctt gttgcgtcgt tcctatgagt ttgggcgtga
    12481 tgagagtttg ctggaatatg ctggtgttat cctcaatacc aaatccatgg atttacatta
    12541 tcaagggcaa gtcttgaatt tgaccaagaa tgaattccag attttacgcg tgttatttga
    12601 gcatgcaggc aacatcgtag cacgtgacga cctgatgcgg gaactttgga acagtgactt
    12661 tttcattgat gataataccc tctctgtcaa tgtggctcgt ttgcgtaaaa agttggagga
    12721 gcagggattg gtaggattta tcgagaccaa gaaaggaata gggtatggat tgaagcatgc
    12781 ttgattggaa acaatttttt ctagcctatc tgcgctcccg tagtcgtctt tttatctatc
    12841 tgctttcttt ggcatttctt gtcttactct ttcagttttt atttgccagt ctaggaattt
    12901 acttcctcta ctttttcttc ttgtgttgct ttgtaaccat attatttttc acttgggaca
    12961 tattggtgga aacgcaggtc tatcgccagg aacttctcta tggagagagg gaagccaagt
    13021 ctcctttgga aatagcttta gcagaaaaat tagaagcgcg tgagatggaa ctctatcagc
    13081 agaggtcaaa agcagaaaga aaactgacgg atttgctgga ttactatacc ttgtgggtcc
    13141 atcagataaa gacccccatt gcagccagtc aactcttagt tgcagaagtg gtcgaccgcc
    13201 aactgaagca gcagctagaa caggaaattt tcaaaatcga ctcctatacc aacctagttt
    13261 tacagtacct gcgtttagaa agtttccatg atgatttggt cttaaagcag gttcaaattg
    13321 aggacttggt caaggaaata attcgtaaat atgctctttt ctttattcaa aaaggcttaa
    13381 atgtcaatct acatgacctt gataaagaaa tcgtgacgga taaaaagtgg ctgctagtgg
    13441 ttattgagca aatcatctca aacagtctca agtacaccaa ggaaggtggt ctggagattt
    13501 atatggatga ccaagagctt tgtatcaaag atacgggaat cgggataaaa aacagtgatg
    13561 tcctccgagt atttgaacgt ggcttttcag gatacaatgg ccgtttgacc cagcagtcct
    13621 ctggacttgg cctttatcta tctaagaaaa tttctgaaga actggggcac cagattcgta
    13681 tcgagtctga ggtcggaaaa ggaacgacag tgcggattca gtttgctcaa gtgaacttag
    13741 tccttgagta aaaatagatg agaaaaagcc tccagattgg aggcttttat gctagactct
    13801 tatttctcaa cgcgtgattt gttggcgata tcagctagga tagcttgaac aaaattatca
    13861 actgagacag tttgtgtttc tttttggccg tagcgacgaa cgttgactgt ttcgtcttcc
    13921 atttctttgt ctccaacaat taattggtaa ggaatcttgc tagtttgtga agcacggatc
    13981 ttgaactgca ttttttcatt gcgctcatct acgtctgcac ggacaccgcg gtcacggagt
    14041 ttcttggcta cttcccaagc gtagtccacg tgtttttcgt tagatactgg gataagggtt
    14101 acttggtgtg gtgccagcca tgttgggaag gcacccttgt agttctcaat caagatagct
    14161 gtgaagcgtt ccatagttga gataacccca cggtggatca tgactggacg gtgatcttcg
    14221 ccatcagctc cgatgtattt gaggtcgaag cgttctggca acaagaaatc aagttggata
    14281 gtagaaaggg tttcttcttt tccaagggca gttttaatct ggatatccaa ttttggtccg
    14341 tagaaggctg cttcaccttc ggcttcaaag tagtccacgc ccatttcatc aagggctgca
    14401 cgaagcatgg tttgggcatt ttcccacatc tcatcgttat caaagtactt atgagtatct
    14461 tgagggtcac gaagagagag gcggaagcgg tagtcagtca agttgaagtc ttcataaaca
    14521 tcgataatca actgaagggc acgttggaat tcttcttgga tttgttctgg agtaacgaat
    14581 aggtgaccgt cgttgagtga catttcacgt acacgttgaa ggccagtgag ggcaccagat
    14641 ttttcgtaac ggtgcatcat accgatttca gcgatacgga ttggcaattc acggtaagag
    14701 tgaacatggt gtttgaaaac ttggatgtgg tgcggacagt tcattggacg aaggacaaat
    14761 tcttccccgt cacccatgtc catggttggg aacatgtctt cttggtaatg atcccagtgg
    14821 ccagaagtct tgtaaagctc aacagaagca agtggtggag tgtagacgtg ttggtagcca
    14881 gaagccaact ctttgtttac gatgtagcgt tccaattcac gacggatagt cgcaccattt
    14941 ggcaaccaga atggcaaacc ttgtcccact tcttgtgaaa tcataaagag gtcaagctct
    15001 ttaccaagtt tacggtggtc acgttcctta gcttcttcac gcatttgaag gtagtttttc
    15061 aagtctttct tgtcaaacca agctgtaccg tagatacgtt gcatcatagc gttgtcgctg
    15121 tttccacgcc agtacgcacc agctacatgg agaaggtgga agatttggat acgacctgtt
    15181 gatggaacat gaggtccacg gcagaggtct acatattcac cctgacgata gatagtcaaa
    15241 ccgccttcgt cttctgagtg ttcttcaatc aattccaact tgtaagggtc atttttgaag
    15301 atttcacgtg cctcgtcttt agtcacttct tcacgaatag atgggaagtt ttctttgacg
    15361 attttttgca tttcttcttc gatacgagga aggtcttcgt tagagatttg accagctgtg
    15421 ttgtcagtat cgtagtagaa accatcttcg atggctggac caactcccaa gtgaatgtct
    15481 gggaaaagac gacgagctgc ttgggcgaac aagtgagctg ctgagtgacg caagattgga
    15541 agggcatctt cgtgatcagg tgtcacaatt tcgatgcttc catcttcagt gatagcgcga
    15601 gtagtgtcga tgagtttgcc gttgaattta ccagccaagg cttttttagc tagggaattg
    15661 ctgatagatt gggcaatttc aaaagttgtt acgccagatt cgaattcacg aacagcgcca
    15721 tctgggaaag taatgttaat catgatgttc tcctttttta ttatttcata gtttctaagc
    15781 attgattttt tatcagcaga ctacttccaa gtcctaacgt tcaattgctt ttctttatca
    15841 gagaggaagt tttccatttc cgtcagaatg tctatatcca aacgttgaga aagttctaat
    15901 agtcaccaga tattttctga aagtttttgt tccagtgtgt agggtgtttc atcatagtag
    15961 cgtccttgtt ttgtcatcac cagtcgttgg aaatttccaa tatcattaga taaagccaag
    16021 aggtcttctt ctaccgtcca cttggaatca tgatgcttaa cttccagttc gttataagct
    16081 tgtcggattg cccaactgcg ctccactaat tcttgcaact ccataattag tatcctaata
    16141 aaaaaatgcg acatcctcga aaggacgtcg caacgtggtt ccaccttcat ttatgtacct
    16201 caaaagagag ggacacctct gattggctct aacgtggcca ccgttttatg ttttcataaa
    16261 aaactcaaga gtagtatcag tttagacttc ctatgcgttt ccagcaacca cgcactctct
    16321 agtagaaagg gactaagtga cttgtctcta tggattatta tagcatgatt gtgagatttt
    16381 tcaaggattt tgtgaaagtt ttttgttttt cattttttgt agaatatgct tgatgctaat
    16441 accagaatcc atgcccacca gagcaactgt ttcatagatt agcaagtaaa ttagtataaa
    16501 ctcaccaaag gttatgatag taaagtaaaa gagcagtccg ttaccaacta accatcaaag
    16561 tgagaaacgg aaacggtagc tatagagtaa cgatacaatc aagattacca gaggtgtaaa
    16621 gaagagaaga ttgtttatat agtgtaaggg ggtcagaaac aactagcaag aacaactcat
    16681 aaagtggcca ataaaagaat aatatgacca gatagtaggg gagatgggaa tagaatctac
    16741 gcatgataat cacctcgttt taaaaaaaca gacaacttag atctcttgct atgattatta
    16801 tagcatattt aaaacaagaa agtaaatttg ttgacaaaga agattttttt tcactagaat
    16861 catggttagt tttcttggta gaaatatttt gataataatt aagcaagact attgacattg
    16921 attattaaga gtgtcacaat tgctgtaact aaaataatta caacaagaga ggtataaaaa
    16981 atgaaactag cagttattgc agcaaatgga caagcgggga aagcaattgt tgaagaagca
    17041 gtcaaacgag gacatgaagt aacggctatt gttcgttctg aaaacaaaag tcaagctgag
    17101 tctattatta aaaaagattt atttgagttg acaaaagatg atcttactgg atttgatgct
    17161 gttatttcag cgtttggtgc ttatacacca gatacgctac cgctccatag caaatcaatc
    17221 gaactattta atcagcttct tgctggaaca cagacacgtt ttctagttgt agggggagca
    17281 ggaagccttt atatagatga aacaaaaacg acacgacttt tagatacgcc tgattttcct
    17341 gaagaattta agccactagc aaaagctcaa gcggatgaat ttgatcttct tcgtacaaaa
    17401 aataatctca actggacttt tgtcagtcct gctgtagatt ttattcccga tggtgaaaaa
    17461 acaggaaact acattctagc aggagaaatt tttactacta atggaaaagg aataagccag
    17521 attagttatg cagattatgc tattggattg gtagatgaac ttgaaaaagg tcatcatata
    17581 aaagaacgta tctctttact tgaaaaataa taactatgag agtattaatg tttgacagaa
    17641 aatttaaatc ttggtaaaat agggattgtc gtaaagacaa ataacttctt cttggttaca
    17701 ggcatgccaa cctgtcactc ggatgaagcc aaataaaaag gagaaacatc atggcaatct
    17761 caaaagagaa aaaaaatgaa atcattgcac aatatgcacg tcacgaaggt gatacaggtt
    17821 cagtagaggt tcaagttgct gtccttactt gggaaatcaa ccaccttaac gaacacatca
    17881 aacaacacaa aaaagaccac gctacttacc gtggattgat gaaaaaaatc ggtcgccgtc
    17941 gtaacttgct tgcatacttg cgtaaaaacg acgttaaccg ttaccgtgag ttgatcaact
    18001 ctctaggact tcgtcgctaa ttcaagatac aaaggccgtc aaaagcacaa agcaaaaata
    18061 ggaaaattga cgaagaaact tcagtttcta ggagatttta tcttttttgc caagtgctta
    18121 ggccgtgttc aattgagcat atcttgataa tgaagctact ctaaattggg tagctttttt
    18181 gcgttgtcac cttacctcga tatactcaag tataatcttt ggttacggtt cctagcactg
    18241 taaggtaaaa taaaccagct tttctccctt cggggagatt ttttttgttt cactgaaagt
    18301 ttcgtacgat cttcgcctcg tcgcctagtc tataaacgat ttatctagca agaatcatga
    18361 tgctaagggc gtcaaaaatc cgtatgaaaa tagggaaatg acacagtgtt cgatgagcac
    18421 aaggagtttc atctttttca ctaggatttt agcccgggct caaatcagct ctctgacttc
    18481 agagggcttt tttattgagg ttttatcggt aacaattttg gagactacaa aaacctcaaa
    18541 cggtatcagc taattacacc ataaaggtgc gcagttactc ggcttttcaa gccgagtaac
    18601 tgtatgcaag cccctagaag agcccactct ttacaaagta aagtatagta tgttatactt
    18661 tacatggaag tagtcactga attccagtta gaaattactt tgtaactacg ttttgaggag
    18721 gagtaaaatg ctttcctacg ttcgacatta cccactagcg atagctaaat taatgtgtct
    18781 gtgctctcct aaaatctgct gatttattac tgactaatac aggagttttt ttttatggga
    18841 cagacaatca tatctgctat tggtgtttat atttccacca gtatcgatta tttaattatt
    18901 ttaattattt tatttgcaca gctatcacag aataaacaga aatggcatat ttatgcgggg
    18961 caatatctag gcacaggctt acttgtaggg gcgagtttag ttgctgctta tgtcgttaat
    19021 ttcgtgcctg aagaatggat ggttggattg cttggtttaa tccctatcta tttagggatt
    19081 cgctttgcaa ttgttggaga agatgcggaa gaagaagagg aagaaattat tgaaagatta
    19141 gaacaaagca aggcaaatca actgttttgg acagttacat tgctgacaat tgcgtctggc
    19201 ggagataatt taggtatcta tataccttat tttgcttcgt tagattggtc acagaccctc
    19261 gtggccttgc ttgtgtttgt aatcggcata attatctttt gcgagattag tcgggtgtta
    19321 tcctctattc cgttaatatt cgagacaatt gaaaaatacg agcgaatcat tgtgccctta
    19381 gtattcattc tacttggact atacatcatg tatgaaaatg gcacgataga gacttttctg
    19441 atcgtgtaga tttttttgtt tcactaggat tttagcccga gctcaaatca gctctctgat
    19501 tttcagaggg ctttttcatt gagtataaat ggacaatctt aaagttactc tattgtagag
    19561 tggcttttga tttgattagg agattcttat aaatatagta aaatgaaaca aaaacaggac
    19621 aaatcgatca ggacagtcaa atcgatttct aaaaatgttt taagaagtag aggtgtacta
    19681 ttctagtttc aatttgatat ataaatgaaa accttagctg tttggccaag gttcatttct
    19741 tctatgcaag ttcttctctt ttcttatctg ggttccagat gaagcttgcg ataaagctaa
    19801 agatgatggt taggatagcg aggataatag caaggatgag ggcagggatg cggataaacc
    19861 accagagtgg atttggtttt ttatcattgt gccattgttt cgtttcttcg tccagacgtt
    19921 ggaattctgt ttgaatacga ttggcgactg tttcaatgcc ttcatcattc attttcttga
    19981 tatctgagat atcgattgga tttccaaagt tcatatcgac acgttcacgg ctaatcaagc
    20041 ccttcaaagt catgggaccg gtgtaggtaa ccggcatgat acggaccttg gccattttgg
    20101 caatcagtgc tgcgcccccc ttgacatcgt ttgagtggcg gctaccactt ggaaacatga
    20161 tgagagagcg gtcacttttt ttgagaacgt tgataggata tttgatggct gaggcgctgg
    20221 gattttcacg gtcgatggga aaggcgccac acatacgaat ccaccaacca aagatacggt
    20281 tggtaaagag ttcttttttt gccataaaga tgaactgttt tggcttggtc gcaaaggcca
    20341 tataaacagg atcccaccag gtacggtgag gcgcaactaa aatataattt tcatcttgat
    20401 taggaatttt atcagtatta tgatagtgag cattgccatt gatggaccat aagagcaata
    20461 caactaatcc acgcaaataa gtataaaaca tgcgatctcc ttcgattgtt ttcttgttat
    20521 tattatacct tatcaaagga gggctggcaa acttttccct tgactagatg catatttggg
    20581 atgaaattag aattctgtta aaaaaaatga tataatagaa tttatggata aaaataagat
    20641 tatgggatta acccaaagag aagtcaagga aagacaggct gagggtttgg tcaatgactt
    20701 taccgcatca gccagtacca gcacttggca aatcgttaaa cgaaatgtct ttaccctttt
    20761 taacgctttg aactttgcca ttgctttggc ccttgccttt gtgcaggctt ggagcaatct
    20821 ggtcttcttt gctgttatct gctttaacgc tttttctggg attgtgaccg agctacgagc
    20881 caaacacatg gtggacaagc tcaatctcat gaccaaggaa aaggtcaaaa ccatccgtga
    20941 tggtcaggaa gttgctctta atcctgaaga attagtgcta ggagatgtca ttcgtttgtc
    21001 tgcaggagag cagattccta gtgatgcctt ggttttggaa ggctttgcgg aagtcaatga
    21061 agccatgtta acgggagaaa gtgatttggt gcaaaaggaa gttgacggct tacttttgtc
    21121 aggaagtttc ctagccagtg ggtcagtttt atctcaagtt caccatgtcg gtgcagacaa
    21181 ctatgctgcc aaactcatgc ttgaggctaa gaccgttaaa cccatcaact cccgtatcat
    21241 gaaatcgctg gacaagttgg ctggttttac tgggaagatt atcattccct ttggtctggc
    21301 tctcttgctg gaagccttgc ttttaaaagg cctgcctctc aagtcatctg ttgtaaattc
    21361 gtcgacagct cttttgggaa tgttgcctaa gggaattgcc cttttgacca ttacttcgct
    21421 tttgactgca gtgattaagc taggtttgaa aaaggtcttg gtgcaggaga tgtactctgt
    21481 tgagaccttg gcgcgcgtgg atatgctctg tctggacaag acgggcacca tcacccaagg
    21541 aaagatgcag gtggaggctg ttcttccact gacggaaact tacggtgaag aggctattgc
    21601 cagcatcttg actagctaca tggcccatag tgaggataaa aatccaacag ctcaagccat
    21661 tcgccagaga ttccaaggtc aagtagtcta tcctatgctc tctaatcttc ccttctccag
    21721 cgaccgcaag tggggagcca tggagttaga aggtttggga atagttttct taggggcacc
    21781 tgagatgttg ctggattctg aggtcccaga agccagagag gccttggaga gaggttcacg
    21841 tgtcttggtc ttagctctca gtcaggagaa attagaccat cacaaaccac agaaaccatc
    21901 tgatattcag gctctagcct tgctggaaat cttggatcct attcgagagg gagcggcaga
    21961 gacgctgaac tatctccgtt ctcaggaagt ggggctcaag attatctctg gtgacaatcc
    22021 agttacggta tccagcattg cccagaaggc tggttttgcg gactatcaca gctatgtcga
    22081 ttgttcaaaa atcacggatg aggaattgat tgctctggcg gaggagacag cgattttcgg
    22141 acgtgtttcc cctcatcaaa agaaactcat tatccaaacg ctgaaaaaag cgggtcatac
    22201 aacagctatg actggggatg gagttaatga tatcctggct cttcgtgagg cggattgttc
    22261 tatcgtaatg gcggagggag acccggcaac ccgtcagatt gccaacctgg ttctcttgaa
    22321 ctcagacttt aatgatgttc ctgagattct ttttgaaggt cgtcgtgtgg tcaataacat
    22381 tgcccacatc gctccgattt tcttgataaa gaccatctac tcattcttgc tcgcagttat
    22441 ctgtatcgcc agtgctttac taggtcggtc tgaatggatc ttgattttcc ccttcattcc
    22501 aatccagatt accatgattg accagtttgt ggaaggtttc ccaccattcg ttttgacttt
    22561 tgagcgaaat atcaaacctg tcgagccaaa cttcctcaga agatccatgc ttcgtgccct
    22621 accaagtgcc ctcatggtcg tgtttagcgt tctttttgtg aaaatctttg gaacgagtca
    22681 aggttggtct gagttagaaa tctcaactct cctctattat ctcttggggt caattggttt
    22741 cttatccgta tttagagcct gcatgccatt cactctatgg cgagtcctat tgattgtctg
    22801 gtcagtagga ggcttcctag ccacagctct cttcccaaga attcaaaaac tgcttgaaat
    22861 tgcaacctta acagaacaaa cgttgcctgt ttatggtgtc atgatgttgg tctttacagt
    22921 gattttcatc ctgactagtc gctatcaagc tagaaaataa agaaagactg caatctgtgg
    22981 attgcagttt ttttaggtac aagattgcca gccgaaatgt ggtataataa gaggtaatag
    23041 agttttggaa agtgagagaa gatgatttca aagagattag aattggtagc ttcctttgtg
    23101 tcacaggggg ctattttact agatgtggga agtgaccatg cttatctgcc tatcgagttg
    23161 gttgagagag gccaaatcaa aagcgctatt gcaggtgagg tggtggaagg tccctatcag
    23221 tctgcggtta aaaatgttga ggctcacggc ctaaaggaga aaatccaagt ccgtttagcc
    23281 aatggcttgg cagcttttga agagactgac caagtgtctg tcattaccat tgctggcatg
    23341 ggtggtcgtt tgattgctag gattttagaa gaaggtttgg ggaagttagc taatgtagag
    23401 cgtttgatcc tccagcccaa taatcgtgaa gacgacttgc gtatctggct acaggatcat
    23461 ggattccaga ttgtagcaga aagcatctta gaagaagctg gcaagtttta cgagattttg
    23521 gtggtggaag caggacaaat gaagctatca gccagtgatg ttcgctttgg tcccttcttg
    23581 tccaaagaag tcagtccagt atttgtccaa aaatggcaaa aagaagctga gaagctagag
    23641 ttcgccctcg gacaaatccc agaaaaaaat ctggaagaac gtcaagttct agtagataag
    23701 attcaagcta tcaaggaggt gccccatgtt agcaagtgaa gtgatccaag cttatgaagc
    23761 cttttgccct caggaatttt ctatggaggg agacagccgt ggtctgcaaa ttggcacttt
    23821 agacaagggt atccaaaggg tcatggttgc cctcgatatt cgtgaagaga cagtggcaga
    23881 ggccattgaa aagggtgtgg acttgattat cgtcaagcac gcgcctatct ttcgtcctat
    23941 caaggacttg cttgccagcc gtccgcaaaa tcagatttac atcgacctga ttaagcatga
    24001 tatcgcagtt tatgtcagcc ataccaatat tgatatcgtt gaaaatgggc tcaatgactg
    24061 gttttgtcag atgctaggaa tcgaggagac gacttatctg caggaaacag gtccagaacg
    24121 tggaattgga cgtattggga atattcagcc tcagacattt tgggaattgg cccaacaggt
    24181 caagcaagtc tttgacctag atagccttcg aatggtgcat tatcaagaga atgatttgca
    24241 gaagcctatt tcaagagtag caatttgtgg tggaagcggg cagtctttct ataaggatgc
    24301 tttggcaaag ggggcagatg tctatatcac tggcgacatc tactaccata ctgctcagga
    24361 tatgctgtct gatggcttgt tagcattgga tccaggtcac tatatcgaag tgctttttgt
    24421 ggaaaaaatc gcagcactcc ttactcaatg gaaagagaaa aaaggctggg aactagagat
    24481 tttacctagt caagcatcga ccaatccttt ccgtcatatc tagttagaaa gtgaagacaa
    24541 tgaaaaaagt tgccattatt ggagcaggaa ttgtgggagc aacagctgcc tactacctct
    24601 cgagagaaag tgacttagag gtgaccgttt ttgaccatga gcaaggtcaa gccaccaagg
    24661 ccgcagcagg aattatcagt ccttggtttt ccaaacgccg taataaagcc tggtacaaga
    24721 tggcgcgctt gggggctgat ttttatgtgg atttattagc tgatttagag aaatcaggac
    24781 aagaaatcga cttttaccag cgttcgggag tctttctttt gaaaaaggat gaatccaagt
    24841 tggaagaact ttatcaactg gccctccagc gcagagaaga atctcccttg atagggcaat
    24901 tagccattct ggaccaagct tcagctaatg aattattccc tggtttgcag ggatttgacc
    24961 gcctgctcta tgcttctggt ggagcgagag tagatggcca actcttggta actcgtttgc
    25021 tagaagccag tcatgtcaag ctggtcaaag aaaaagtgac tctgacaccg ttagcatcag
    25081 gctaccagat tggcgaagag gtgtttgatc aggttatttt ggcaacggga gcttggttgg
    25141 gggacatgtt agagccttta ggttatgaag tggatgtccg tcctcaaaaa ggacaactac
    25201 gagattatca gcttgcccaa gacatggaag attaccctgt tgtcatgcca gaaggggagt
    25261 gggatttgat tccctttgca ggtgggaaat tatccttggg cgctacccat gaaaatgaca
    25321 tgggatttga tttgacggta gatgaaccct tgctccaaca aatggaggag gccaccttga
    25381 ctcactatct gattttggct gaagctactt caaaatctga gcgtgttgga atccgtgcct
    25441 acaccagtga tttctctcct ttctttgggc aggtgcctga cttaactggt gtctatgcag
    25501 ccagtggact aggttcatca ggcctcacaa ctggtcctat tattggttac catttagctc
    25561 aactgatcca agacaaggag ttgaccttgg accctgaaaa ttacccaatt gaaaactatg
    25621 tcaaacgagt aaaaagcgaa taatactcaa tgaaaatcaa agaacaaact aggaaactag
    25681 ccgcgggtta ctcaaagcac tgctttgagg ttgtggatag aactgacgaa gtcagtaacc
    25741 atacccatgg caaggcgacg ttgacgtggt ttgaattgga ttttcgaaga gtataagcat
    25801 tttactgaaa ttttatcagg tagtttagga tggcaaatga cattccctgt caaaaatggt
    25861 aaaataagaa aaaataatct gagaatcgag gaaaaaagat gcaagaaaag attttggtaa
    25921 ctggtggtgc cggttttatt ggaacccaca ctgttattga attgatccaa gcaggccatc
    25981 aagttgttgt ggtggataat cttgtcaata gcaatcgtaa gagtttagaa gttgttgaaa
    26041 gaatcacagg agttgagatt cctttctatg aggcagatat tcgtgacaca gataccctca
    26101 gagatatttt caagcaggaa gaaccgactg gtgtcattca ctttgctggt ttgaaggctg
    26161 ttggcgaatc aacacgtatc cctcttgcct actatgacaa caatatcgct ggaactgtca
    26221 gccttttgaa agtcatggaa gaaaacaact gtaaaaacat catcttcagt tcttctgcga
    26281 cagtttacgg ggatccgcac acagtgccca tcttggaaga tttcccactt tcagtgacca
    26341 acccatacgg tcgtactaag ctcatgctag aggaaatttt gactgatatt tacaaagcag
    26401 actcagaatg gaatgttgtc ttgcttcgtt actttaaccc aatcggagcc catgagagtg
    26461 gtgatttggg agaaaatcca aacggtattc caaacaatct cttgccatat gtgactcaag
    26521 tagccgttgg aaaattagag caagtgcaag tgtttggaga cgattacgat acggaagatg
    26581 gaacaggtgt tcgtgactat atccacgttg tcgatttggc taagggtcac gttgcagctt
    26641 tgaaaaaaat ccaaaaaggt tcaggactaa acgtttataa ccttggaact ggtaaaggtt
    26701 actcagttct tgaaattatc caaaacatgg aaaaagcggt gggacgtcct attccttacc
    26761 gcatcgtaga acgtcgccca ggtgatatcg ctgcctgcta ctcagatcca gcaaaagcta
    26821 aagcagaact cggttgggaa gcagaactcg acatcaccca aatgtgtgaa gacgcatggc
    26881 gttggcaaag caagcatcca aatggatttg aagactaaga tgatgatttc aatcatcgtc
    26941 ccttgtttaa acgaagagga agtacttcct cttttttatc aagctctgga agctttactt
    27001 ccagatttgg aaacagaaat cgagtatgtc tttgtcgatg atggatcgag tgatgggacc
    27061 ttggaactct taaaggccta tcgggagcaa aatccggcag cccattatat ttctttctcg
    27121 cgaaattttg gcaaagaagc agccctttat gcaggcttgc aatatgcgac aggagatttg
    27181 gtggtggtga tggatgcaga cctccaagat cctcctagta tgttgtttga gatgaaaaat
    27241 gtactagaca aaaatgtaga cttggactgc gttgggacac ggagaactag tcgggaggga
    27301 gaacccttct ttcgcagttt ctgtgctgtt ctcttttatc gcctcatgca aaaaatcagc
    27361 ccagtagctc tgccgtcggg tgtccgtgat tttcgtatga tgagaaggtc tgtggtcgat
    27421 gccattttaa gcttgactga gtccaatcgt ttttcaaagg ggctcttcgc ctgggtgggt
    27481 ttcaaaacct actatttgga ctatccaaat gtcgaaaggc aggctggcaa gaccagttgg
    27541 agttttaggc aactcttttt ctactccatt gaagggatta ttaatttttc agattttccc
    27601 ttgattatag cctttgtagc tggtctccta tcttgttttc tttctctgct gatgaccttt
    27661 tttgttgtgg ttcggaccct cattttgggc aatccgacat ctggttggac ctctctgatg
    27721 gctgttattc tctttcttgg aggcattcaa ctcttgacca ttgggattct cggaaagtat
    27781 atcagtaaga tttatttaga gactaaaaaa agaccacttt atcttatcaa agaaaaaagt
    27841 gaccttcctg attttacaga aaaaaataaa gtgaaaagac tataatttta catggaaata
    27901 tgctaaacta gagggagtgg attatcgcca ttgatttagg cttcctggtt tccaatcagg
    27961 gcgcgagttg ggcaattttt aacagcctct aagacatcct gactaggaga aatttctttt
    28021 tccagttggt cagggtcatc gtaaaaacgc acgattccat tatcgtggta atcaaataaa
    28081 tcagaataag tttggcaaag cccacaggcg atacatcgtt caggtataag tgtgattttc
    28141 atgttcatat ttatattgta ataagaaaag taaaaaaaaa caaggagtaa ggtatggcaa
    28201 aagaaccgtg gcaagaagat atctatgatc aagaagaatc aagagcagag cgtcggcatc
    28261 gaaaccacgg aggggctgat aggatggcta atcgtatttt gacgatccta gctagtattt
    28321 tctttgtaat tgtggtggtg atggtcatcg ttctcatcta tctatcatcg ggggggagta
    28381 atcgcacagc agccttaaaa gactttcatg attctgatgc aagtgtagta caaatctcat
    28441 cttcaagcag ttctcagcct gagcagagtt cagagccaga atctacttct agtagttcag
    28501 aagaagctgc taatcctgaa ggaacgatta aagttctcgc aggagaaggg gaagcagcta
    28561 ttgccgctcg tgcaggaatc tccattgctc agttagaggc cttgaatcct gggcacatgg
    28621 ctacaggatc ttggtttgct aatccaggtg atgttataaa aataaaatag gagccgatat
    28681 gaaaacaatt caaattgcta ttgatggtcc tgcttccagc ggtaagagta cggtcgcaaa
    28741 gattattgct aaggattttg gattcaccta ccttgataca ggagctatgt atcgtgcagc
    28801 gacctatatg gctcttaaga accaattagg agttgaagaa gtcgaagccc ttctagcctt
    28861 gttggaccag catccaatca gctttggacg ttcagaaact ggagaccagc ttgtttttgt
    28921 aggagatgtg gatattaccc atcctatccg tgaaaatgaa gtgaccaatc atgtttctgc
    28981 tattgcagca attcctgaag tgcgtgagaa actggtttct ctccaacaag aaattgccca
    29041 gcaaggcggg attgtcatgg atggtcgcga tattggaact gttgtattgc cacaagcaga
    29101 attgaaaatt ttcctagtag cttctgttga tgagagagca gagcgtcgtt acaaggaaaa
    29161 tattgccaag ggaattgaaa cagaccttga aaccctaaaa aaggaaattg ctgcgcgtga
    29221 ctacaaggat agtcatcgtg agacttctcc tctcaaacaa gcagaggatg ctgtctacct
    29281 tgatacaact ggtttgaaca ttcaagaagt agttgaaaaa atcaaagcag aagctgaaaa
    29341 aagaatgtaa atgtagaaaa gccgatagat tgatatcggt ttttttctaa atttgtcaaa
    29401 atgctgattt tagggtataa tagttgctgt tcgagaaatt ttgattttca aagaatagtg
    29461 aatgatgata aggagaaacc atgaacaatt taccaaattg tccaaaatgt aactcagagt
    29521 atgtctacga agacggtgcc ctactggttt gcccagagtg tgctcatgag tggaatcctg
    29581 ctgaagttgc agaagtagaa gaaggtcttg tcgctatcga tgccaacgga aataaattga
    29641 ctgatggtga tacagtaact ctcatcaagg acttgaaagt aaaaggtgcg ccaaaagatt
    29701 tgaaacaagg gacgcgcgtg aaaaatatcc gcatcgtaga aggcgaccac aatatcgact
    29761 gtaagattga tggttttggt gccatgaaac ttaaatcaga gtttgtgagg aagatttaag
    29821 catgtcaaaa cactataaac ttgtatttta tagccgtatc ttcttgtttc tagcggcttt
    29881 tacgggagtt tatcttgaaa ttactaggca tggtggtttt gggatgcttc tctattacac
    29941 ggttctgtcc aacctcttgg taactatttt taccctttat cttctaaagg ttatgagccg
    30001 tgtaggtgaa aattggcaaa ggccaagtct cttgcgctta aaaggtgggg tcaccatgag
    30061 tatcatgatt acctgtgtga tttaccattt cctcttggcg cccattgcga ctaatttcta
    30121 taccctagaa aatttccttt gccactatat cgttcccatc tggtttttag cggataccct
    30181 cttttttgac aaacagggtc aatacaagat ttgggatcca gcagtgtgga ccattttacc
    30241 ctttttgtat atgatgtttg ctctttttaa tggcttggtt ctaaaactca atattccaaa
    30301 tgccaaggat aatcctttcc cttacttctt tttgaatgtg aacaagggtt ggaatgttgt
    30361 gtttaagtgg tgtctgatta tctttgttgc ctatatggta gcaggattta ttttctactt
    30421 tatcaagcaa atcaagagaa agtcatccta atactcttcg aaaatctctt caaaccacgt
    30481 cagtttccat ctgcaacctc aaaacactgt tttgaacaac ctgcggctag cttcctagtt
    30541 tgctctttga ttttcattga gtataagata caagggattc attttcgaat ttaagaagga
    30601 gtctacaaac caatgaggct tcttcttttc ttgcttattt gataactatc aaaaaagtag
    30661 aagattaaga gaaatataga aagagatttc atgaaacaat ttttagaacg ggccagcatt
    30721 ttggctctct ccctcgtttt gattacctcc ttttccattt cgagtgctct accagccatg
    30781 tttgactatt atcagggtta ttctaaggaa caaattgagc tcttggtgag cttgccttcc
    30841 tttggaatca tgatgatgtt actgctaaat ggtttcttag aaaaaatatt tcctgagcgc
    30901 ttacagatta gtttgggctt gctgatttta tcattgagcg gtacagctcc cttctggtac
    30961 caagcctatc cctttgtctt tggaacacgg cttctctttg gtttgggtct tgggatgatc
    31021 aatgccaagg ccatttctat tatcagtgaa cgctaccaag gaaaaaggcg aattcagatg
    31081 ttagggctac gcgcttctgc agaggtcgtt ggagcttctc tcattacctt ggccgtcggt
    31141 cagttgttgg cctttggttg gacagctatc tttctagcct atagtgctgg atttttggtg
    31201 ctgccccttt atctgctctt tgtcccttat ggaaaatcaa agaaagaagt caagaaaaga
    31261 gcgaaggaag caagtcgttt aactcgagaa atgaaaggct tgatttttac cttagctatc
    31321 gaagcggcag ttgtagtttg taccaataca gctattacca tccgtattcc aagtttgatg
    31381 gtggaaagag gattggggga tgcccagtta tctagttttg ttcttagtat catgcagttg
    31441 atcgggattg tggctggggt gagtttttct ttcttgattt ctatctttaa agagaaactg
    31501 ctcctctggt ctggtattac ctttggcttg gggcaaattg tgattgcctt gtcttcatcc
    31561 ttgtgggtgg tagtagcagg aagtgttctg gctggatttg cctatagtgt agtcttgacg
    31621 acggtctttc aacttgtctc tgaacgaatt ccagctaaac tcctcaatca agcaacttca
    31681 tttgctgtat taggctgtag tttcggagcc tttacgaccc catttgttct aggtgcaatt
    31741 ggcttactaa ctcacaatgg gatgttggtc tttagtatct taggaggttg gttgattgta
    31801 atctctatct ttgtcatgta cctacttcag aagagagctt aggattgatt cctaagtttt
    31861 tcttttgata ctcaatgaaa atcaaagagc atagttgatt gagtttggaa tagtacgctg
    31921 tagttgctaa aatatttcta gaaattaatt tgacttttct aatagagtta ttcatatctt
    31981 atttcaatct actatagaaa gctagccgca ggttgctcaa aatagtgttt tgaggttgca
    32041 gatggaaact gacgtggttt gaagagattt tcgaagagta tgagattttt atacccatac
    32101 aatatttgtt ttaggtcctt gtttacagac taatttcttg ataaaataga ggttatgacg
    32161 agatataaag caactatttc ctatgatggt tatgcctttg ctggctttca gcgccagtct
    32221 catgcgcgta gcgttcagga agaaattgaa aaaaccttga ccagattaaa taaagggcaa
    32281 accattactg ttcacggtgc tggtaggaca gatagtgggg ttcatgccct gggacaggtc
    32341 attcattttg acctgcctta tcagatggat gaggagaaac tccgttttgc cttggatacc
    32401 cagtctcctg aagatattga tgtgatttcg attgagcttg tggcagatga ttttcattgc
    32461 cgttatgcca aacatagcaa gacctatgag tttattttgg atagaggacg tcccaaaaat
    32521 cctatgcgcc gtcactatgc cacccacttt ccctacccac tcgatgtgga acgaatgcag
    32581 attgcaatca aaaagctaga gggaacccat gattttaccg gttttacagc ctctgggact
    32641 agtgtagagg ataaggttcg caccatcaca gaagctagtt taatagtcga tgagacagga
    32701 caatttttga cctttacctt ttcaggaaat ggtttcttgt ataaacagat tcgcaatatg
    32761 gtggggactc ttctcaaaat cggtaacaac cgcatgccag tagagcagat tgatctcatc
    32821 ttggagaaga aggacaggca acttgcaggt cccactgcag caccaaatgg tttgtattta
    32881 aaggagattc gttatgaaga ataatcgtat tttagcactt tctggaaatg atatttttag
    32941 tggtggtgga ctgtcagctg atttggctac ctataccttg aacggcttgc atgggtttgt
    33001 agcagtgact tgtttgacag ccttgacaga aaaaggattt gaagtctttc caactgatga
    33061 taccattttt caacatgaat tagatagctt gcgtgatgtg gaatttgggg gaattaagat
    33121 tggtcttctc cctactgtca gtgtggctga gaaggccttg gactttatca aacaacgccc
    33181 aggagtacct gtggtgttgg atcctgtctt ggtctgcaag gaaacgcatg atgtagctgt
    33241 cagtgagctc tgccaagagt tgattcgctt tttcccttat gtcagtgtga ttacgcctaa
    33301 tctcccagaa gcagaattat tatccggtca ggaaattaaa accttggaag acatgaaaac
    33361 tgcagcgcag aaattgcatg atttaggagc gccagcagtc attatcaagg gaggcaatcg
    33421 tcttagtcag gacaaggctg tggatgtctt ttatgatgga cagaccttta ctatcctaga
    33481 aaatccagtt atccaaggcc aaaatgctgg tgcaggttgt acctttgcct ctagcattgc
    33541 cagtcacttg attaaaggtg ataaactttt gccagcagta gaaagctcta aggctttcgt
    33601 ttatcgtgct attgcacaag cagatcagta tggagtaaga caatatgaag caaacaaaaa
    33661 caactaaaat cgcccttgta tccctattaa ccgccctttc tgtggttcta ggttatttct
    33721 taaaaatccc aacacctaca ggaattctaa ctcttttaga tgctggtgtc ttctttgcgg
    33781 ccttttactt tggtagtcgt gaaggagcgg tagtcggagg actagcaagt ttcttgattg
    33841 acctcttatc aggctaccct cagtggatgt tctttagctt ggtcaaccat ggcttgcagg
    33901 gatttttcgc aggatttaaa ggaaaaagtc agtggttagg ccttatttta gcaactattg
    33961 ccatggtagg aggctacgcc ttgggttcta ctttgatgaa tggctgggca gcagccctcc
    34021 cagaaattct accaaatttc atgcaaaata tggtagggat gattgtagga tttattctta
    34081 gtcaaagtat caagaagatt aagtaatact cttcgaaaat ctcttcaaac cacgtcagtt
    34141 tccatctgca acctcaaaac actgttttga gcagcctgcg gctagctttc tagtctgatt
    34201 ttcattgagc ataaagaggt tgagtaaaaa gtctttcaaa taagaaaaac gcatagtata
    34261 ggatgttgaa atgatgaact gccccccaaa agttagacag aaaaaatcta acttttgggg
    34321 tgtttttatt atgaaactaa cttatgatga taaagttcag atctatgaac ttagaaaaca
    34381 aggatatagc ttagagaagc tttcaaataa atttgggata aacaattcta atcttaggta
    34441 catgattaaa ttgattgatc gttacggaat agagttcgtc aaaaaaggaa aaaatcgtta
    34501 ctattctcct gtttaaaaca agaaatgatt aataaagtct gacatgaagg ctagactaaa
    34561 gatagagttt ctcttgaata cggtctccca agtcgtacga tacttcttaa ctggctagca
    34621 caatacagga aaaacgggta tactattgtt gagaaaacaa gagggagagt acctaaaatg
    34681 ggacgtaagc ccaaaaagag acctgaagag aggacagaat tagaacgtct tcaagcagaa
    34741 aatgagtacc tgagagcgga gaatgccatc ctaaaaaagt taagagaact ccgattgaag
    34801 gaggaaaaag agaaagaaga aagacagaaa ttgttcaaga attaatgact gagttttcgt
    34861 tagatcttct tctaaaagcc attaaactag ctcgttcgac ctactactat cacttgaaac
    34921 agctagacaa accagataag gaccaagagc ttaaagctga aattcaatcc atttttatcg
    34981 aacacaaggg aaattatgct tatcgccggg ttcatttaga actaagaaat cgtggttatc
    35041 tggtaaatca taaaagagtt caaggcttga tgaaagtact caatttacaa actaaaatgc
    35101 gacagaaacg aaaatattct tctcataaag gagacgttgg taagaaggca gagaatctca
    35161 ttcaaggcca atttgaaggc tctaaaacaa tggaaaagtg ctacacagat atgacagaat
    35221 ttgccattcc agcaagtact caaaagcttt acttatcacc agttttagat ggctttaaca
    35281 gcgaaattat cgcctataat ctttcaactt cacccaactt agaacaagta caaacaatgt
    35341 tggaacaggc attcacagag aagtactacg agaatacgat tctccatagt gaccaaggct
    35401 ggcaatatca acacgattct tatcatcggt tcttagagag taagggaatt caagcatcca
    35461 tgtcacgcaa gggaaacagc caagacaacg gtatgatgga gtccttcttt gggattctga
    35521 aatttatggc tatgagaaaa catttaaatc acttaatcaa ttggaacaag ctattgtaga
    35581 ctatattgat tactacaaca ataaacgaat taaggtaaaa ctaaaaggac ttagtcctgt
    35641 gcagtacaga actaaatcct ttggataaat taattgtcta acttttgggg ggcagtacat
    35701 tttaacactt gataatatgc gttttattgt gggaagattt tcctcatttt ctagtgaaat
    35761 tgaacttttt ctaacatctt ttgatagatg acttcttaat caattatcat ttccattgtt
    35821 gtttcttggg gtttcatccc ttgtctttga taaaagcgga gagcaccctc gttatcattc
    35881 cagacactta gggttagatt attgcagcca atttctttag cataatagca gaaaaatgac
    35941 aataaaatag cccctagata ttttgaaagg aaagcatagg ggctatatag tttaatcaaa
    36001 ataaagcaaa tctttgctgg tgcttgtaaa gaggtcgaag ccatccttgg taacaacacc
    36061 gcagtcttca atacgaacac cgactttacc agggatatag atacctggtt caacagagaa
    36121 gcacatgcct tcttcgatga ccatgtcgtt tccttccatg atagatggga attcatggac
    36181 atccataccg ataccatgcc cgagacggtg gttgaagtac tcaccataac cagctttttc
    36241 gatgatctca cgggcagcgc ggtccacttc atgagcagtc acacctggct tgataaagtc
    36301 aagagcagct tgttgggctt caagagtcaa gttgtaaata tctttcttga attggtctgg
    36361 tttgccgaca gcgactgtac gagtcatatc tgacgcatag ccattgacca gaacccccag
    36421 gtcaaagagg agaagagcat cattttcaac cttattagct gctggaatgc cgtgtggatt
    36481 cgcagcatta tcaccagtca agaccatggt atcaaagctc atttcgtaac cttcacgttt
    36541 catggcaaag tcaatttgtg cgatgatatc tgtctcagtc ttatcaagag aaatattgtc
    36601 aaaaccaaca tgaacagcct tgtcagcata aagacctgca accatcattt tttgtacttc
    36661 atcagctgat ttgatgaggc gcatgcgttg gatacgagga gtgaggtttt caaactcagc
    36721 agtttcaaaa attgttttca aaccatggta tttggtcaag atgagattgt caaactcaac
    36781 agcgacacgt ttgaagtcaa gttgtggaag agcatgtttg attttttgcc atggattttc
    36841 agaatcgaca tagcccacta ctgggaagga aacggtgcta cttgcacgtt ctacttcaag
    36901 agctgggaca aagaggagag gttcctgatc cgctaggaca aagaggaaca tttggcgttc
    36961 atggggatca ctgtaaaaac cagtgaggta attgattgtg acggggtcag atacgacagc
    37021 gacgtctagt ttttctgatt caagatatgt taggatttgt tgtaatttag acatatgcta
    37081 ccttctttct acccctctat tttggcaaaa aatgagagaa aatgcaaggg agaagggtaa
    37141 aagaaaagac aaaaaactcc gacaagataa cggagttctt tgatatcatt ttcctttgtt
    37201 taagaaaagt cagccttgct tttgacgtca ctgtactctt cagcgatttc ttggctgagg
    37261 atatcctcat aggcagggag ttggatatcc tgaccatatt tcttcttgag ggcagtagta
    37321 actaggcgat aagccagatt ggcattacga gagaggatag gcccgtggaa gtaggaacca
    37381 aagacattct tataatgaac cccttcaccg accttttctt cgttgtttcc atttccatag
    37441 acaacttgtc ccagcggttt ttggtcatca gagaggaagg tacggccttg gtgattttca
    37501 aatccatagt aggtttcatc gaaatcttca ttgtgaatct tgatgtcacc gataaaacgg
    37561 ttattggtct ggttgagcgt gtagtgtccc atgaccccta gcccttcgat acgttttcct
    37621 gaagcttcaa cataatattg acccaatagt tggaaaccac cgcagatagc cagaactaca
    37681 ccgtcgtttt ggatgtagtt gtcaatgctc tcttttttag caggtaggtc gtctgcaatg
    37741 atactttgtt caaagtcttg accaccaccg aaaaaggcga tgtcgtagtg attttcatca
    37801 aagtcatcat ggagagaaac gatgtcaacg gtcacatggg ctcccagttt ttcagccaca
    37861 tacttgagca tgaggatgtt tccattgtcc ccgtaggtat tcatgagatt tccgtagagg
    37921 tgggcaatgt tgagctgata ggggtaattg ccatcttttg aggaaagtga agtataaacc
    37981 attagttcat ctcctttcta acaatctgac gactagccag cagttcacga aattccagca
    38041 tggcagtata agttgccaga atataggcat gcttgcagtc ttgattctca atggtcttga
    38101 gaacttgctc cagattactc gtttcagtga ttttctcagc tggatagcca gtcactcgga
    38161 ggcgacgagc gatttcagaa tgacgaacac cgccagcgtt gatttcagga atgtccatgt
    38221 cagtgatttg ctcaaagtct gcatcccaga tccagctagt gtcaattcca tctgcatagt
    38281 tggcattaag gaggacagat aggctaaatg gataaggtgc tagtttgatc atttcgatag
    38341 cttgggttgc accgactgga tttttaatca agacaagggt acattccttg tcaccgatat
    38401 gaaaggtttc ttggcgtcca aagacagcac ggctcttgtc aaatccctgt ttgatgagtt
    38461 gcgaatcggc acctaggaaa cgggcgatgg ccacagcagc tagggcgtta tatatattat
    38521 agagcccgcc gatttggata ccgtattctt ggccgtctat gacaaagcga gagcgattgt
    38581 tggtcaactc aaccaatttt gtcaaacgat agtcgagatc aggacgttta catccacaac
    38641 cttcacagat ataggcaccc aagtttgcat aggtattatg ctcatatttg aggatgcctt
    38701 ggcagtcagg acagagaatc ccttcggtat tgtagtgagc cagttgggct ggtccttttt
    38761 ccaagtcaaa accaaaatac tctatagggt ttggaatagt tggcttgtag aaaagtggac
    38821 tgtctccgtt aaggagaaca gtagcagttg gaactttccg aatggcatcc aatatcatgt
    38881 tataggtagt atagatttca ccgaaacggt ccatctggtc acggaagata ttagtaatga
    38941 caaaaagact aggctggata tagtcacaga tacgagatag actggcttcg tcaatttcga
    39001 ggacggcaat attttttcca gttttagaag atttggctgt taggaaggtt gttgcaatcc
    39061 ctgtaatcat gttggcaccg cttgggttgg ttagaacttg accataaacc tcttttaaaa
    39121 tgccgacagt gagggcagtt gtcagggttt ttccatttgt tccagtgaca acgacaatct
    39181 cgtagttctt agctaggttt tgtaaaatat ctttatcaaa ttgaagggcg actttacctg
    39241 ggagcgtact tccacgtcca agacggctta aaacgaagtg ggaagaacgc ccagcaagaa
    39301 ggcccaaagt agtttttaag ttcatgtttc tattatatca caaaagaggg aaaagacaga
    39361 tttggtgttt cgtagaagta aaaacagccc aaagagggct gtcagttaag atgagatttc
    39421 aacttaaatc gcaaacaagt cattcaagtt ctcagccaag gttgggtgag tgaagatttg
    39481 ttttgtgaag taagtgtaag gaatcttgtt gtccatagca acagtaatga tgttgatgat
    39541 ttcttgagaa ccttctgaga agatgcttgc accaagaatt tcttttgttt cagtattaac
    39601 aacagctttg aaagctccgc gaaggtctcc atttacgtga ccacgaggca tggctgcaac
    39661 agggatttct ttcactgcgt atggaagttt caaatcagct gcttggcttt cagtcaaacc
    39721 aacttgtgaa agtgcaggtg tgatgaacat agtatttggc acattgagac ggtcctcaag
    39781 tgtgtagctg ccatctccag caaggtagct gtaaacaaca cggaagtcat caagtgaaat
    39841 gtaagtaaat tgaagaccac cgttgacatc tccaactgca aagacaccag gaacgtttgt
    39901 ttgacagtgt ttatctactt taatagcgcc acgttcagtt agttcaatat ctgtattttc
    39961 aagttgaagt ggttctacat ttggtttgcg tccagttgcg tagagaaggg cgtcgaaacg
    40021 gtaagtttcg tcttcagtta cgacaagcac ttggtcaccg tcgtttttaa tttcagtagt
    40081 atggatattt tgaagcaatt caataccgtc ttcttccatg tattgtttag caagagctgc
    40141 gatggaaggt tctgcacgag gtaggaatgt atccaaggca tctaggactg taaccttgct
    40201 tcctagttta ttgtaaaggc cagcaaattc aagaccgata tttccgccac caaggactcc
    40261 aagtttttca ggcaatttat ccaagctttg gatacctgtt gagtcaaaga cgtttttgct
    40321 tgtagcaagt ccagggattg gcaagacgtt tgaaacagca ccagtgttga tgacgatagt
    40381 ttcagcagtc aattcttgtt tttcatcacc agcttggatt tcgatgactt tatttgaaag
    40441 gaagtgagct tcagcatcaa agatattgac gccagtacca gcaacagtcg tatagttttt
    40501 accgttgagg cgaccagtga tcgtgttttt ggtagcaatg acttcttcaa aagacaagtc
    40561 tttctcagca gcaaccagca aagttttagt tgggatacaa ccgatgttga tacaagttcc
    40621 accgtacata gctttgctac gttcaacgag ggcaactttt ttgccagctg aagccaattt
    40681 acctgctagt gttttaccag ctttaccaaa tccgataacg attaaatcat aagttaacat
    40741 ttagagttcc tcctattcgc atttcattct agcacaagaa aaaaactttt gcacaattct
    40801 gctacggaaa agacagaaaa agataaaaac gctttccaga caaaaaacta gtaatttttt
    40861 gacaagcttg ataaactaaa atcctatcta tttttatata agatagaaaa aatgttcgaa
    40921 aataggaggt tttatgaaat cgaatcgtta tattattgcc tttgctgggg tcattttaca
    40981 cttaatgctg ggttcgactt atgcttggag tgtttatcgt aaccctatta ttgaaaaaac
    41041 gggatgggat caggcttctg ttgcctttgc ctttagtcta gcaatctttt gtttgggctt
    41101 atcggctgca tttatggggc gtttggtaga aaaatttggt ccgagagtca tggggagtct
    41161 atctgctttt ctatacgcag gtggaaatat cttaacagga tttgcaatag accgtcagga
    41221 actgtggttg ttgtatctag cttatggcat tttaggtggg cttggtttgg gagcaggcta
    41281 tattacccct gtgtcaacga ttataaaatg gtttcctgat aaacgtggtc tcgcaacagg
    41341 tttagcgatt atggggtttg gttttgcttc tttattgact agtcccatag cgcaacatct
    41401 catcgcaggg gtagggcttg tagaaacttt ttatatttta ggtgcaagtt actttattat
    41461 catgctccta gcttcacaat tcattaagcg tccaaatgag caagagcttg caattttatc
    41521 tgcttcaggg aaagaaaaaa cagactctct gacgcaaggg atgactgcaa atcaggctct
    41581 aaaaagcaat cggttttata tgctttggat tattttcttt atcaacatag cttgtggttt
    41641 aggcttaatt tcagcggcat cgccaatggc acaggagatg gctggcttgt ctacaagtca
    41701 tgcagcagta atggtgggta ttttggggat tttcaatgga tttggtcgct tgctctgggc
    41761 gagtttgtct gactatatcg gtcgccctct aacctttagt atattactgc ttgtcaatct
    41821 tttcttttct ctctcacttt ggctctttac agattccgtt ttatttgtag ttgctatgtc
    41881 tattttgatg acttgctatg gagctggttt ttctttgatt ccagcttatc tcagtgatat
    41941 ttttggaacc aaggaattgg ccgctctgca tggttatatt ttaacagctt gggcaatggc
    42001 tggtttagcg ggacctattt tattagcaga gacttataaa atggctcatt cgtacacaca
    42061 aaccttgttc gtttttctca ttttatacag tatcgccttg gctttgtctt attatctagg
    42121 tcgttcaatc aaaaaagaaa gtcaaaaacc gcttacatga ttttctgtag aaatggggct
    42181 atcttttgct ctggtcttgt gttatactag ataggttgca aagaaaacag tacttttctt
    42241 ttgtggaaaa gaagcaacac gattttatat ctagttatat gaaaatacgt cataaaaaga
    42301 aaagtataaa ctaagcccta tagggtggct tcgcaccacc tttagaaaga agaataacgt
    42361 gaaatttaat gaattaaact tgtctgctga tttgctagca gaaattgaaa aagctggttt
    42421 tgtagaagct agtcctatcc aagaacaaac tattcctttg gcccttgaag gcaaggatgt
    42481 tatcggtcaa gctcagactg gtacaggaaa aactgcagcc tttggcttgc ctacccttga
    42541 aaaaatccgt acagaagaag cgactatcca agctttggtc atcgctccaa ctcgtgaact
    42601 agctgtccaa agtcaagaag aactcttccg ctttggtcgt agtaagggag tcaaagtccg
    42661 ttcagtatat ggcggatcaa gcattgaaaa acaaattaag gctcttaaat ctggtgccca
    42721 tatcgtggtg ggaactccag gtcgcctctt ggacttgatt aaacgcaagg ccttgaaatt
    42781 acaagacatt gaaaccctta tccttgacga agcggatgaa atgcttaaca tgggcttcct
    42841 tgaagacatc gaagccatta tttcccgtgt acctgagaac cgtcaaactt tgcttttctc
    42901 agcaactatg ccagatgcca tcaaacgtat cggtgttcag tttatgaaag cccctgaaca
    42961 tgtcaagatt gcggctaagg aattgacaac agaattggtt gaccagtact atatccgtgt
    43021 taaggaacaa gaaaaatttg acaccatgac tcgtctcatg gatgtggcac aaccagaact
    43081 cgctattgta tttggtcgta ccaaacgccg tgtggatgaa ttgactcgtg gtttgaaaat
    43141 tcgtggcttc cgtgcagaag gaattcatgg cgacctagac caaaacaaac gtcttcgtgt
    43201 ccttcgtgac tttaaaaatg gcaatcttga tgttttggtt gcgacagacg ttgcagcgcg
    43261 tggtttggat atttcaggtg tgacccatgt ctacaactac gatattccac aagatcctga
    43321 gagttatgtt caccgtatcg gtcgtacagg tcgtgctggt aagtcaggtc aatctattac
    43381 ttttgttgct ccaaacgaaa tgggttacct tcaaatcatt gaaaacttga ctaagaaacg
    43441 catgaaaggt ctcaaacctg caagtgtaga agaatccttc caatcaaaaa aacaggtagc
    43501 tctcaagaaa atcgaacgtg attttgcaga tgaaaccatt cgtgccaact ttgagaaatt
    43561 tggtaaggat gctcgcaaat tggctgctga gtttactcca gaagaattgg caatgtatat
    43621 cttgagtctg acagtccaag acccagatag ccttccagaa gtggagattg cacgtgaaaa
    43681 accactaccg tttaaaccat caggtaatgg tttcggtggt aaagctaagg gaggtcgtgg
    43741 aggccgtcgt ggggacgacc gtcgagagcg tgatcgccgt ggcaatggtc gccgtgatga
    43801 gttcaaaaaa ggaagtcgtg gcaacgatcg ttttgataag gaaaaacgtt accgtaagga
    43861 taataaaaaa ccacgcaata ctttaagcga aaagcaaaca ggctttgtta ttcgtaacaa
    43921 gggcgataaa taagaaaaag cggttcaaaa tgaatcgctt ttttatataa ggagaccgaa
    43981 tgtgaaaaga caaataaaat tattatatta tcttttataa tgttatgata cacaaaaata
    44041 ggcaacttgt caatagaaaa ggaacaattt aattaaaaat attctggttt ttaattaaat
    44101 tgtaagt
//
LOCUS       DAVKJJ010000011        41947 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000011 DAVKJJ010000000
VERSION     DAVKJJ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41947
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.011"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(221..1855)
                     /locus_tag="VW144_000595"
     CDS             complement(221..1855)
                     /locus_tag="VW144_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600080.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLFMFINIAFRFPFEEMPSFFSTMVAILLVLAFSTSFTAFYNVFYESK
                     DLASYRPYAFKESEIIMAKGLSVLLPALAGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVTLVMVVAVHFLAQTRVFRKYRSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEDKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVVTVLFTSAFFPYL
                     MMIGLISKIRDSQIVPDIHPPYWLPLFFIALFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIYAVQSFLPVLTLLGLSLYLGLPIISMIYLIVAWILASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLST
                     ILAISLGVGALILLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(1836..2585)
                     /locus_tag="VW144_000596"
     CDS             complement(1836..2585)
                     /locus_tag="VW144_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9600081.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLTSSDLETSLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCNRIAILKKGHLIYCGSVEN
                     LRKDHPDQSLESIYLSLAGRKEEVADASQGH"
     gene            2764..4218
                     /locus_tag="VW144_000597"
     CDS             2764..4218
                     /locus_tag="VW144_000597"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEU9600082.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNDFHWHWYHFT
                     GTDYDAKRRKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLNY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFIDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(4308..6926)
                     /gene="alaS"
                     /locus_tag="VW144_000598"
     CDS             complement(4308..6926)
                     /gene="alaS"
                     /locus_tag="VW144_000598"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU9600083.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GNIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDAKEVNGHRYIASQLSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGR
                     NQAKIQELLDAVAGKL"
     gene            complement(6948..7433)
                     /locus_tag="VW144_000599"
     CDS             complement(6948..7433)
                     /locus_tag="VW144_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEU9600084.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(7708..7947)
                     /locus_tag="VW144_000600"
     CDS             complement(7708..7947)
                     /locus_tag="VW144_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600085.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8208..9278)
                     /locus_tag="VW144_000601"
     CDS             complement(8208..9278)
                     /locus_tag="VW144_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9600086.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9275..10048)
                     /locus_tag="VW144_000602"
     CDS             complement(9275..10048)
                     /locus_tag="VW144_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9600087.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(10045..10851)
                     /locus_tag="VW144_000603"
     CDS             complement(10045..10851)
                     /locus_tag="VW144_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9600088.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10832..11989)
                     /locus_tag="VW144_000604"
     CDS             complement(10832..11989)
                     /locus_tag="VW144_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9600089.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(12048..12998)
                     /gene="murB"
                     /locus_tag="VW144_000605"
     CDS             complement(12048..12998)
                     /gene="murB"
                     /locus_tag="VW144_000605"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU9600090.1"
                     /translation="MSVREKMLEILEGIDIRFQEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR"
     gene            complement(13110..13637)
                     /locus_tag="VW144_000606"
     CDS             complement(13110..13637)
                     /locus_tag="VW144_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEU9600091.1"
                     /translation="MIYGLAMAAFFEETARLVFFKWLEKKRSLEKADALAYGLGHGGL
                     ELIFLGLTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQSLSVWQIYLLGFERILAL
                     GFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLSQVGWLTNPVLVEVILALEL
                     VLVAYGTKEIFCKKS"
     gene            complement(13672..13863)
                     /locus_tag="VW144_000607"
     CDS             complement(13672..13863)
                     /locus_tag="VW144_000607"
                     /inference="COORDINATES: protein motif:HMM:NF021592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEU9600092.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKRR"
     gene            complement(13873..14604)
                     /gene="budA"
                     /locus_tag="VW144_000608"
     CDS             complement(13873..14604)
                     /gene="budA"
                     /locus_tag="VW144_000608"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEU9600093.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14746..16017
                     /locus_tag="VW144_000609"
     CDS             14746..16017
                     /locus_tag="VW144_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU9600094.1"
                     /translation="MKKQSKYKEVVSYLKNGIESERFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEERVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(16037..16852)
                     /locus_tag="VW144_000610"
     CDS             complement(16037..16852)
                     /locus_tag="VW144_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9600095.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(17009..17662)
                     /gene="phoU"
                     /locus_tag="VW144_000611"
     CDS             complement(17009..17662)
                     /gene="phoU"
                     /locus_tag="VW144_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9600096.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(17674..18432)
                     /gene="pstB"
                     /locus_tag="VW144_000612"
     CDS             complement(17674..18432)
                     /gene="pstB"
                     /locus_tag="VW144_000612"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9600097.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(18445..19248)
                     /gene="pstB"
                     /locus_tag="VW144_000613"
     CDS             complement(18445..19248)
                     /gene="pstB"
                     /locus_tag="VW144_000613"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9600098.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(19259..20143)
                     /gene="pstA"
                     /locus_tag="VW144_000614"
     CDS             complement(19259..20143)
                     /gene="pstA"
                     /locus_tag="VW144_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9600099.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(20133..21050)
                     /gene="pstC"
                     /locus_tag="VW144_000615"
     CDS             complement(20133..21050)
                     /gene="pstC"
                     /locus_tag="VW144_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9600100.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(21050..21928)
                     /gene="pstS"
                     /locus_tag="VW144_000616"
     CDS             complement(21050..21928)
                     /gene="pstS"
                     /locus_tag="VW144_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU9600101.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(22502..23806)
                     /locus_tag="VW144_000617"
     CDS             complement(22502..23806)
                     /locus_tag="VW144_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEU9600102.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(23807..24580)
                     /locus_tag="VW144_000618"
     CDS             complement(23807..24580)
                     /locus_tag="VW144_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU9600103.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSSTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRSVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(24570..24848)
                     /locus_tag="VW144_000619"
     CDS             complement(24570..24848)
                     /locus_tag="VW144_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEU9600104.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(24848..25249)
                     /locus_tag="VW144_000620"
     CDS             complement(24848..25249)
                     /locus_tag="VW144_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEU9600105.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(25459..26076)
                     /locus_tag="VW144_000621"
     CDS             complement(25459..26076)
                     /locus_tag="VW144_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEU9600106.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLTLVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            complement(26066..26839)
                     /locus_tag="VW144_000622"
     CDS             complement(26066..26839)
                     /locus_tag="VW144_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEU9600107.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(26850..27587)
                     /locus_tag="VW144_000623"
     CDS             complement(26850..27587)
                     /locus_tag="VW144_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEU9600108.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKV"
     gene            complement(27592..28722)
                     /locus_tag="VW144_000624"
     CDS             complement(27592..28722)
                     /locus_tag="VW144_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEU9600109.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(28806..29195)
                     /locus_tag="VW144_000625"
     CDS             complement(28806..29195)
                     /locus_tag="VW144_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU9600110.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIHITEE"
     gene            complement(29211..29594)
                     /locus_tag="VW144_000626"
     CDS             complement(29211..29594)
                     /locus_tag="VW144_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEU9600111.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(29595..30383)
                     /locus_tag="VW144_000627"
     CDS             complement(29595..30383)
                     /locus_tag="VW144_000627"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU9600112.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(30376..31311)
                     /locus_tag="VW144_000628"
     CDS             complement(30376..31311)
                     /locus_tag="VW144_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEU9600113.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINIADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(31441..31617)
                     /locus_tag="VW144_000629"
     CDS             complement(31441..31617)
                     /locus_tag="VW144_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU9600114.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(31757..32464)
                     /locus_tag="VW144_000630"
     CDS             complement(31757..32464)
                     /locus_tag="VW144_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEU9600115.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESNLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            32615..33643
                     /gene="queA"
                     /locus_tag="VW144_000631"
     CDS             32615..33643
                     /gene="queA"
                     /locus_tag="VW144_000631"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU9600116.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGSHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQRIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHSIQEHYRFFS
                     FGDAMFIY"
     gene            complement(34192..35319)
                     /locus_tag="VW144_000632"
     CDS             complement(34192..35319)
                     /locus_tag="VW144_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_135354310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9600117.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGSIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGHVTEKVTVD
                     RKEATDTVIKVPAKSKVEEVLVPFTTKYEADNDLSAGQEQEITLGKNGKTVTTITYDV
                     DGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDDPTLDKGQEVGEIG
                     KITLTTIYAVDERDGTIEETTSRQITKEMVKRRIRRGTREPEKVVVPEQSSIPSYPVS
                     VTSNQGTDVAVEPAKAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGS
                     MKVNQWFQVGGKWYYVNTSGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(36035..36586)
                     /locus_tag="VW144_000633"
     CDS             complement(36035..36586)
                     /locus_tag="VW144_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9600118.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGHGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(36833..37657)
                     /gene="nadE"
                     /locus_tag="VW144_000634"
     CDS             complement(36833..37657)
                     /gene="nadE"
                     /locus_tag="VW144_000634"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEU9600119.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(37654..39114)
                     /locus_tag="VW144_000635"
     CDS             complement(37654..39114)
                     /locus_tag="VW144_000635"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU9600120.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(39230..39718)
                     /locus_tag="VW144_000636"
     CDS             complement(39230..39718)
                     /locus_tag="VW144_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600121.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYIIYLPWIFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(39708..39917)
                     /locus_tag="VW144_000637"
     CDS             complement(39708..39917)
                     /locus_tag="VW144_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9600122.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            complement(40302..41031)
                     /locus_tag="VW144_000638"
                     /pseudo
     CDS             complement(40302..41031)
                     /locus_tag="VW144_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(41047..41838)
                     /locus_tag="VW144_000639"
     CDS             complement(41047..41838)
                     /locus_tag="VW144_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9600123.1"
                     /translation="MNFALNKGEIVGQVGRNGVGKSTLMKILVQNNQPTSGNIISSDN
                     VGYLIEEPKLFLSKTGLENLKYLSNLYGVDYNQERFRSLIQELDLTQSINKKVKTYSL
                     GTKQKLALLLTLVTEPDILILDEPTNGLDIESSQIVLSVLKNLALHENVGILISSHKL
                     EDIEEICERVLFLENGLLTFQKVGKDSHNFLFEIAFSSATDRDIFITKQEFGDIVQEE
                     GLRITMSGNIQSSELFKFFNENSIKVVDFETKKETLKDIYLNRSK"
BASE COUNT        12615 a         9450 c         7803 g        12079 t
ORIGIN      
        1 atacttggaa cacacacatt ataggaatta acgtttttga aattgaaaaa tatccaaata
       61 aataaactat aaacaacaaa aatagaacta tgttatattt cttattcaaa acattcctcc
      121 ctatattttt gattaccaat cttaatcatt tacaactaca ttctaacaaa ctataaaagc
      181 gtttgtcgaa ttgaatttat caagcaagcg accaaccagc tcatcttttt tctatttctg
      241 ccaatatgcg tgacaggtaa taatgatagc caaaaatagc aagagcaagc aggaggataa
      301 gagctcctac tcccaagctg atggcaagga tagtggagag agactgaacc aagaatatgc
      361 tcccaattac aagggccatc aggattgcac tataaataaa caataaaact atggcgacta
      421 tgccatttga acgattcacc aggtccgtaa tgctactcca attggttgac agatttttaa
      481 cgtccttaaa gtaatggtgg caagaaagga tgacactggc aaggatccat gccacaataa
      541 ggtaaatcat cgaaatgatg ggcaagccta gatatagaga aagaccaagc aaagtcagaa
      601 ctggtaaaaa ggactggaca gcatatataa tccaaaattt cactttcaca taacgagcaa
      661 agtcaaaggg taaactctta agaaaatcaa cattttccct ctccaaggac aaggcaattg
      721 aatgcaggct ggtgatattg ttattgacaa ctgctataaa gagagctata aaaaacaagg
      781 gtaaccagta tggaggatga atgtctggaa ctatctgaga atctcggatt ttggaaatca
      841 gaccgatcat catgagataa ggaaagaaag cacttgtaaa aagcactgta accacgccag
      901 tcccctgtcc caagagggtg aggtggtagc gtaaaaccat gcgaaaaaat ccctttttag
      961 tggttgaaat tctctccttg ctgcgacgtt cttttttgac cttatcctca ctattaagca
     1021 ggatcacgtc ataaaaacga ggaaggacct tctttttggt cagataaagc aggaagagag
     1081 ttagtcctat ccaagcgagc agacccacta aggcttctgt cgaaaaaggc tccactgcta
     1141 ttttgtaaaa gatatgaaga ggataaagga gaaatggaat gtctctaact ttgtcaacaa
     1201 tacttccaaa agtcgactga agaaagaaga taaatattaa aggtatgaga actcctatcc
     1261 caatcatcac attcgaaaaa atagaccgat actttctgaa gaccctagtc tgagccaaga
     1321 aatgtactgc cactaccatc actaaagtaa cagagacaaa taataaggtc aaggacagta
     1381 gcatcaaagg caaacccaac cacagagaag gtgctagcct aatgtagagg accagaaaat
     1441 aagctaggat tggtacaatt ccagctagag ctggcaagag gacagacaga cctttggcca
     1501 ttataatctc tgattcttta aaggcatagg gcctatagga ggccaggtcc ttactctcat
     1561 aaaagacatt gtaaaatgca gtgaaagaag ttgaaaaggc cagcaccagt aaaatagcaa
     1621 ccatcgtact aaaaaaactt ggcatttcct caaaaggaaa acgaaaggct atatttataa
     1681 acatgaaaag catcaagaga ctagaaaaaa tgtaagaact taggactcta gcggaaacat
     1741 ttactttttt cccaggattc ttagcctgct tctttcgtag attagccaga ttagcttctt
     1801 gagatgaata gaggatattg atatcaacta attttttaat gaccttgaga cgcatccgca
     1861 acctcctctt ttctaccagc aagactaagg tagatacttt ccaaagactg gtctgggtgg
     1921 tctttcctca agttctctac actaccacaa tagatcaaat gccccttttt caaaatggca
     1981 atccgattac agacttgctc ggctacctct aggacatgag ttgaaaacaa gaccgtcttc
     2041 cctttttgtg catgttcctt catcatctgt ttcaaatcaa aggcagcctg gggatccaaa
     2101 ccagtcaagg gttcgtccaa gacccaaata tcaggatcag acaagagtgc cccgatgaca
     2161 aaaactttct gacgcattcc atgagaaagg gtttcaataa cctgataacg attttcagca
     2221 aaatcaaaaa cgttcaatag cctagctaga ctagtctcca agtcagagct agtcagatca
     2281 taggatgagg cgatcaattc ccaaaattca ttggccgtta aacgtaaaaa taagtcaggc
     2341 gagtctgcta cgtagccaat ctttcgtttg atcgccaagc gattttccga taactcctga
     2401 ccatctacca aaatacgacc actgctgggt gaaatgatac tgactaggga ttttatggtg
     2461 gtcgattttc cagccccatt atgaccaatc aagcccataa tctctccatt ttcaatctgc
     2521 aaattgagat tactcaaggc ctctttatca ccatacaact tactaacatt ttgaaattca
     2581 atcatgggat gactcctatc tttatctgta ttatatatcg ctatactagc actttgatac
     2641 aaaaaaatca atactttatt ttaagtagtt aaaatagttt ccttctatct tacgcaaacg
     2701 tttgctccga gagatacttt atgatagaat aaagaacaag attgacaagt aagaggaaac
     2761 atcatgcaaa atcaaacact catgcaatac tttgaatggt atctgcccca cgacggtcaa
     2821 cactggacgc gtctggctga aaatgctcca cacctagctc atctggggat cagtcacgtc
     2881 tggatgccac cagccttcaa ggcaaccaac gaaaaagatg tcggctatgg ggtctatgac
     2941 ttatttgact taggagagtt caaccaaaaa gggactgtcc gcaccaagta tggtttcaaa
     3001 gaagactatc ttcaagccat tcaagccctt aaagcgcagg gaattcaacc tatggccgat
     3061 gtagttctca accacaaggc tgctgctgat cacagggaag cctttcaggt tattgaagtg
     3121 gatcctgtag accgtacagt tgaacttgga gaacccttca ccatcaatgg ctggactagt
     3181 tttaccttcg atggtcgcca agatacctac aatgacttcc actggcactg gtaccacttc
     3241 acaggtacag actatgatgc caaacgccgt aaatccggga tttacctaat ccaaggagac
     3301 aacaagggct gggctaacga ggaattggtc gataacgaaa acggaaacta cgactacctc
     3361 atgtatgccg acctagactt taaacatcct gaagtcatcc aaaacatcta tgactgggct
     3421 gattggttca tggaaacgac tggtgtagct ggtttccgtt tggatgccgt taagcatatt
     3481 gactctttct ttatgcgcaa tttcatccgc gatatgaagg aaaaatacgg tgacgatttc
     3541 tatgtttttg gtgaattttg gaacccagac aaggaagcca atctgaacta tctcgaaaaa
     3601 acggaagaac actttgacct tgtcgatgtt cgtctccacc agaatctctt tgaagccagt
     3661 caagctggcg caaactatga ccttcgtggt attttcacag atagcctggt tgaactcaag
     3721 cctgacaagg ctgtgacttt tatcgacaac cacgataccc aacgaggaca agcccttgag
     3781 tctaccgttg aagaatggtt caagccagca gcctatgccc tcattttgtt acgccaagac
     3841 ggccttccat gtgtctttta cggagactac tatgggattt caggccagta tgctcaagaa
     3901 gatttcaaag aaatccttga ccgcctccta gccatccgaa aagacttggc ctatggagaa
     3961 caaaatgact actttgacca tgctaactgt atcggttggg tacgttcagg tgctgaaaat
     4021 caatccccaa tcgcagtcct tatctcaaat gaccaagaaa acagcaagtc aatgtttgtc
     4081 ggtcaagaat ggactaatca aacctttgta gatttacttg gtaaccacca aggtcaagtt
     4141 acaattgatg aggaaggtta tggacaattc cctgtctcag ctagatccgt aagtgtctgg
     4201 gcagtcaata ccatctaata gctcataata accaagctag gtccaagcgg atttggcttt
     4261 tttgtattca caaaaagacc tacccaaatg gatagatctt tacttgatta caatttacct
     4321 gctactgcat ccaacaattc ttggatctta gcttggtttc ttcctcctgc catggccatg
     4381 tctggtttac caccaccacg tccatcgatg attggtgcta attctttgac aaggtttcct
     4441 gcatgaaggt cttttgtctt gcttgctaca aggacattga ctttgtcacc gatagcggca
     4501 actaggacaa gaagatcaga gtagtctttt tgtttccagt tatctgcaaa agtacgaagg
     4561 gcaccggcat cggatacaga caattgacta gcaatgtaac gatgaccgtt gacttcctta
     4621 gcatctttga agatatcgcc tgcggctgca gctgcggctt tttctttcaa ctcagcattt
     4681 tctttttgaa gttgacgaag ttgttcttga agtccttcta ccttgtgagg tacttccttg
     4741 acttgaggtg ctttcaaggt tgctgcgaca gctttaagag catcctcttg ttcacgatag
     4801 gcttcaaagg cttccttacc agtcactgcc aagatacggc gagttcctga accgattcct
     4861 tcttctttga caattttgaa gagaccaatc tcagaagtgt tgccaacatg agtaccacca
     4921 caaagttcaa tagagtagtc accgatagtc acgacacgaa cttccttgcc gtatttctca
     4981 ccaaagaggg ccatagctcc catttcttta gcagtgtcaa tatccgtttc aactgtcttc
     5041 acttcaagtg cttcccaaat tttctcgtta acttgctgtt caatcgcacg aagttcctca
     5101 gcagttactg cttggaagtg ggtaaagtca aagcgaagga attcaacttc gttaagagat
     5161 cctgcctgtg ttgcgtggtt tccaaggata ttgtgaaggg cagcgtgaag caaatgagtc
     5221 gcagtgtggt ttttcatgac acggtgacgg cgattgctat caattgccaa ggtatattct
     5281 tggttcaagg caagcggtgc aaggacttca actgtatgaa gggcttgacc atttggggct
     5341 ttctgaacat tggtcacagt agccacaacc ttacctgact catccaagat ttgtccgtag
     5401 tcagctacct gtccacccat ttcagcataa aatgacgttt ccgcaaagat aagagaggca
     5461 gttccttctg aaacagctcc tacttctgca ttgtcagcaa cgatagctac caatttagaa
     5521 gacaattggc tagcattgta gttgaagaca ctttctacag tgatgttttg aagagtttca
     5581 ttttgcatac ccattgagcc acccttgaca gctgacgcac gcgcgcgttc ttgctgttct
     5641 ttcatggctg cttcaaaacc ttcacggtct acagtcatac cagcttcttc agcaatttct
     5701 tcagtcaatt caactgggaa tccgtatgta tcatagagtt taaagacatc ttgaccagca
     5761 atgacagatt gacctttttc tttcaagtct gccacaatgc cttgggcaaa gtgttgacct
     5821 gagtgaaggg tacgggcaaa tgattcttct tcgctcttaa cgattttctc aataaagtca
     5881 cgtttctcaa gcacttctgg gtagtagctt tccatgatgt ttccaacagt tggaacaagt
     5941 ttgtaaagga aaggctcgtt gatacccaat ttttgaccat gcatagaagc acgacggaga
     6001 agacgacgaa gaacataacc acgaccttca tttcctggaa gggcaccatc accgatagca
     6061 aatgaaagag aacgaatgtg gtctgcgata accttgaagc tcatgttgtc gccatcttgg
     6121 tcataaacct taccagacaa tttctcgact tcacggataa tcggcataaa gaggtccgtt
     6181 tcaaagttgg tcttagcccc ttggataaca gccaccaaac gctccaaacc agcgcccgta
     6241 tcaatgttct tatgtggcaa ttccttgtat tcgctacgag gaacagcagg gtctgcgtta
     6301 aattgtgaca aaacgatgtt ccagatttca atataacggt cgttttcaat atcttctgca
     6361 agcaggcgaa gaccgatatt ttctgggtca aaggcttccc cacggtcaaa gaagatttct
     6421 gtatctggtc cagaaggtcc cgcaccgatt tcccagaagt tgtcctcaat tggaatcaag
     6481 tgacttggat ccactcccac ttcaatccag cggttgtaag aatctttatc gtctggatag
     6541 taggtcatgt aaagtttttc agcagggaaa tcaaaccatt cagggcttgt caaaagctca
     6601 taagcccaag tgatagcttc gtcacggaag taatccccga tagagaagtt ccccaacatt
     6661 tcaaacatgg tatggtgacg cgcagtcttc cctacgtttt cgatgtcgtt ggtacggata
     6721 gccttttggg cattggtaat acgtggattt tcagggataa tggtcccgtc aaagtatttc
     6781 ttaagggttg ctaccccaga gttgatccac aaaagagttg ggtcatttac aggaaccaaa
     6841 cttactgatg gttctactga gtgacctttg gtcgcccaga aatcaagcca catttggcgt
     6901 acttgtgcac tagatagttg tttcatattg tctccttatt tacttgttta atgtgattgg
     6961 ctttccagca tttccacata gtcaatcgcg acacagaggg aaatgactag gtctgcataa
     7021 gcgtcttcaa gaaccgttac ggtataggta gaggtcagat ggaagagttc cttcttaatt
     7081 tccgcaatca actgatcgcg atcatccagc aatttgaaat tcaaatccca gatattgccc
     7141 tcgatacgaa gacctagatt atcaaactca tacttatctc gccagaaggt caacttctta
     7201 cgaatgacaa aactcgagcc atcccgaagc tgaatctcaa aacgaggaag caaggtcaag
     7261 atttctttac taatctcact gacttgttca ccagccgcat catagatggt aaaggtttta
     7321 ggaatcttaa aaaatgatcc ctccacctga taggcaattt ctcccctgtc atccttgata
     7381 gcgaagcgtt cgcctccaag acgaaacttt tgtttgacaa gaaatgtttt catcaacacc
     7441 tccaaaaatc aaaagacaag ctcatatcac gaagggcgaa aaaccgcggt accaccttca
     7501 ttcaatgaac ttgtcattct cttgttctta tgcaattgta tgattgagca gcatgacttc
     7561 ctagcttaga tggctcgcag caccgccatt tctctggact aagacaagtg aaaatcaatt
     7621 ctcaactttc ttattataac gtttttttaa gcttgcgtca actggaaatg atctccgttg
     7681 aattagacca attccctaca tctctgatta ctttttcagg atatattttt tcttactgcc
     7741 gtttttcttt ttatcccaaa ttttcatatt actaaacaca gctactagaa tatttccaaa
     7801 tataaaggtg cctatcaccc aatatatgga ctcagttgtt aggtattgtc gatccaagcc
     7861 atcctttaaa tggaatagta tagcagtttg gttaacaatc ataaaggttg gccagaaact
     7921 ttttttgaaa aaagtagaca ttttcattat ttgttgccgc tttctgtaag gttaatactc
     7981 aataaaaatc aaaaagcaaa ctaggaagct agcctcaagc tgtacttgag tacggcaagg
     8041 caacgctgac gtggtttgaa gagtataggc ttagtatact actaggcaag caaataagca
     8101 aataaacaaa taaacaacta gaatagaaaa agatagggct ctaaaaactg acttctattc
     8161 cttaaaaacg aaccagcttg actgattcgt cttcttacgt ttatctccta cttccgatac
     8221 attttaaact gtaggaagag gtcgctatat ttccctgtcc atttatggtc aaatttctca
     8281 taaacttcta ggtgtttcat ggtttcaaca tcgggataga aggccttatc ttcctttgtt
     8341 tcctctggga gcaattcctt cgctggtagg tttggtgttg aatagccgac atactccgca
     8401 ttttggagag cattttcagg tttcaacata aagttgataa aggcataggc tgagttttgg
     8461 tttttaactg ttttgggaat gaccatattg tcaaaccaaa gattgctggc ctctgtcggt
     8521 accacataac gtagattttc atttttttct aacatttggc tggcttcacc agagaaggtc
     8581 acgccgattg caacattatt ctgaatcata tagcccttca tctcgtccgc aacgatagcc
     8641 ttgatatttg gagtcagttt gtagagctta tccactgtct cttccaactg ctgcagatcc
     8701 ttggagttga gactgtagcc gagggaattg agtcctagtc ccagcacctc acgcgcccca
     8761 tcaaagagca tgatagaatt cttatactcc ggcttccaaa ggtcatccca atgctcaggc
     8821 gcttcatcta ccatggtttc gttgtagaca attcctaagg ttccccagaa gtaagggatg
     8881 gagaatttat tacctgggtc aaaggactgg ttgagaaact ctggtccgat attttcgatt
     8941 ccttcaattt ttgaataatc aagcggaacc aagaggtctt cgtccttcat cttgttaatc
     9001 atgtattcac ttggaatggc aatatcgtag gtcgttccac cctgctttat cttagtgtac
     9061 atggcttcgt tggagtcaaa agtctcgtac tgaacttgaa ttcctgtttc ttctgtaaac
     9121 tgagtcaaga gttcaggatc gatatagtct ccccagttat agataaccaa tttttgacta
     9181 tctcgactat tgattttact atctaaatga gtcgcaattc cccacaagac aaggataatc
     9241 gctgcaattc ctgctaaaaa tgaatagatt tttttcatgc ttgctcctcc ttctcacgag
     9301 agataaagta ataacctaca actaggataa tactaaagag aaagactaga gcagacaggg
     9361 cattgatttc taaggaaatc cccttgcgag cacgagagta aatctcgact gatagggttg
     9421 aaaagccatt tcctgttaca aagaaggtca cggcaaagtc atctaacgaa taggtgaagg
     9481 ccatgaaata accagtaatg atagacggag tcaggtaagg aagcatgatt tccttgaaca
     9541 tctgaaattg actagctccc aagtcatagg ccgcatgaat catgtcgcca ttcatttcct
     9601 tgagtcgagg caagaccatc aagaccacga taggaatgga gaaggccacg tgactagata
     9661 gaacggtcaa aaagccaagt gaaaacttga gttgggtaaa gagaatcaag aagctagcac
     9721 caatcataac gtcaggcgca accatgagga tattattgag tgatagaaag gcttcttggt
     9781 atttcttacg agactggtag atgtaaatgg caccaaaagt cccgataatg gtcgctatca
     9841 aagctgatag gaaggccaag aaaaatgtct gagccaaaat cagcatgagt ctcccatctc
     9901 caaacatggt ttcaaagtga gtccagctaa aacctgtaaa gctattcata tcatcaccag
     9961 cattaaaggc atagccaatc aagtaaaaga taggcaggta gaggaccaga aagaccagtc
    10021 ccagataaag gttggcaaat tttttcatcg ttctctcctt tccttagtca cccacatggt
    10081 gatgaacatg gtcaggatga gaatcacacc gatggttgaa cccataccat agttgtcatt
    10141 ggttagaaaa ttctgctcaa tagccgtccc caaggtgata acgcggttcc caccaatcaa
    10201 acgggtcagc atgaagagac tcaaacttgg gataaagacc gactgaaccc cacttcgcac
    10261 accgttcata gatagaggga agatgacatg acggaaggtc tcccacttag ttgcaccaag
    10321 gtcataacta gcattgatga gattattatc catatcgtct aagacattga aaatcggcaa
    10381 aatcataaag gggagctcga tgtagcttgc gacaaagata aaggaaaaat cggtaaaaag
    10441 caactgttgt gaaccaattc cgataaattc caagaattgg ttaatagagc cattttgacc
    10501 aaaaatcccg ataaaagcat aggctttaag gagcaaattg atccaggtag gaaggataat
    10561 cagcatgagc cagagttgac ggtgcttgag acgggtcaaa aagagggctg ttggatagct
    10621 gataagcaag gtcacaaagg tcacaatgcc tgcataaagc actgagttga aactcatttt
    10681 gaggtaggtc aagttttgtg acgcaaagta agatttataa ttttctaaac taaactggcc
    10741 ttcaatgttg aaaaaggatt gtccgaaaat caagaccaag ggtgccaata caaagagggc
    10801 aatccaaagc atgtagggca ctacaaagag tttagagctt gttttcttca tctctttcct
    10861 cctcgatcgc attgatcaga cctgcttctt gctcttcgat ttccacgtat tcttcgatac
    10921 gagcatcgaa ctcttcttcg gtttcattga gacgcatgat gtggatgtct tctgattcaa
    10981 agtccagacc gatttcctca cccacgatgg ccttacgggt tgagtggatc atccattcat
    11041 ttccaagttc gtcataggcg ataatttcat aatgaactcc acggaagagc tgggtgtcga
    11101 ccttaacttg gagtttgcct tcttcaggaa gggtaatgcg caagtcctct ggacgaataa
    11161 cgacctcaac aggttcattt ggcttcatcc caccatcaac cgcttcaaag cgtttgccgt
    11221 taaattcgac caagtagtcc tcaatcatgg tacctggcaa gatgtttgac tccccgataa
    11281 aggtggcaac aaagtggttg attggctcat cgtagatgtc cacaggggtt ccagactgga
    11341 caatctcgcc atcattcata acgaaaatcc agtcactcat ggcaagagct tcttcctgat
    11401 cgtgagtgac aaagacaaag gtaatgccca atcgttgttg taattcacgc aattcgtact
    11461 gcatgtctgt tctcaatttc aagtccagcg ctgataaagg ctcgtccaac aagaccacac
    11521 ggggttggtt gatgatagca cgggcgatgg ccacacgctg acgttgtcct ccagaaagtt
    11581 tgcggatgga acgtttttca taaccttcca actgaaccat cttgagaact tccgctacac
    11641 gctgctcgat ttctttctta tcaattttac gcaagcgaag tggaaaggca acattttcaa
    11701 acacattcat atgtgggaac aaggcatagg attggaagac ggtatgtacg tcgcgcttgt
    11761 tggttggaat atcattgata cgaacaccgt ctagcatgat atctcctgtc gtcgcatcca
    11821 gtaaacctgc aataatgttt aggatagttg atttccccga accagatgca cctagaaggg
    11881 tgtagaattt cccttcttcc aactcaaagt tgatgtcttt gagaaccttg gtgttgctgt
    11941 cttcaaaaac tttagagacg tttttgaatt cgataattgg ttttttcaat tggcataaat
    12001 tccttctttt tcatagatta accgatcggg gctctgtcag gtccccacta cctcttgcag
    12061 ggagtaaaac cacctgcata catcttcgct accgataggc tttcacccaa gatccggact
    12121 tctctttcaa gcgtaatacc tgagtgttcc ttgacttttt cgataaccga ttggatcaag
    12181 tcctcgtagt ctttggccgt tccatctgcg acattgatca taaatcctgc atgcttttct
    12241 gacacttcta cgccaccgat acgatagcct ttcaagccag cttctgaaat taactgacct
    12301 gcaaaatgcc cgactggacg cttaaagacc gagccacaag atgggtattc caaaggttgc
    12361 ttgagttcac gtaggtgcgt caagcggtcc atttcctgct tgataacctg atgggttcct
    12421 ggagctaggg caaatttaac tgacaagaca actgcaccag actcctgaat agctgaatgg
    12481 cggtaaccaa aagccaagtc tttagcagac agggtttcga tttctccatc cttggtcaag
    12541 accttacaag actgcaagat gtgagcaatc tcgccaccat aggcacccgc attcataaag
    12601 acagcaccgc caacgcttcc tggaatacca caagcaaact caaagccagt taaactatga
    12661 cggagggcaa tgcgagttgt ttcaatcaag ttagccccag cttctgcttc aatggtatag
    12721 ccatcaacag aaacgttatt gagcttgtca cacaagatga caaatccacg aatcccacca
    12781 tcacgaacga tgatattgct tgcattgcca agaaccatcc aagggatatt ttcttggttg
    12841 gcaaatttca caacgcgagc caactcaaaa cgatttcgtg gaaagaccaa ataatcagcc
    12901 tctccaccta cttttgtata actatagcta tgcaagggtt cctgaaaacg gatatcaatt
    12961 ccttctaaga tttcaagcat tttttctctt acagacatgt cactcttcct tttacaaaat
    13021 tcattccatt ataccatttt tagagacatt tgacgaccat aaaaatacct tgtttggatt
    13081 ttgcataaga aaaagaggtt tccccctttt tatgattttt tacaaaagat ttccttggtt
    13141 ccataggcga ccagaacgag ctccagtgct agaatcactt caaccaagac tggatttgtc
    13201 aaccagccta cttgggaaag agatggtgcc agatcaaaga aggcatgcaa gccataggct
    13261 gctaggagat aaatccattt cttctggcga acagcttggt aaacccaaac tgtcaaaagt
    13321 aattggaaac caagcgccaa gattcgctca aaaccaagca aataaatctg ccagaccgaa
    13381 agtgactgaa tggtttttaa catattttca gacagtaatt gcataacctg tggattctga
    13441 gtttgaactg ccgaaagaac aatgtaaaga ttgagtaaac tagtaaggcc tagaaaaatc
    13501 aactccaagc caccatgccc cagtccatag gccaaggcgt ctgccttttc caaacttctc
    13561 tttttctcca accatttgaa gaaaacaaga cgagccgttt cctcaaaaaa tgctgccata
    13621 gctaggccat agataatata gacaagcggg tgatcttgca agagggcaat actaccgtct
    13681 tttttgagga tgcaaaatca agatatgcac cagtttttcc aaaatctgcg aagagacaaa
    13741 gaaagcgact gcacccaagc ccaagacagc tagattaatc tggtatttct ttttggcaaa
    13801 ccaaatgctt cctatcagaa aagctagcaa cagcaacatg gtaataatga tatgaatggt
    13861 cattttcttc tcctattctg ccttttcaat atcttttttc atctcgtcaa cattgaactt
    13921 agcaaacaag tattgacggt cttggactgg gaaacgttgg tccaactggt caactgctcc
    13981 cacctcgata atgccttcct tgatgacaaa atccatgaca tgtccaccga aggtcaaatc
    14041 atctgatatg aagtgcagat ggtagcctgc cacactgacc ccatggaaaa tctcaggcgt
    14101 ccagaaacca acaatagtcc ccgcaacatt gtcacgacta tattccggtt gatgggttgc
    14161 gacatcagca aacttggtat cgggtgttga cttaggaatc atacgcacat gcatatgtga
    14221 aaattccccc cgaatcttga tagaacggaa aagattttcc ccatcataat aagactcaat
    14281 tcgttctttc aattccttgt ctgtcatctc aaagcgctgg cggaaaatga cctctgcctg
    14341 atgcggtacc actgcagcgt aaggaataag ggcatctggt gacacttcca caatttctgg
    14401 ctggtctcct gaccctttgg cctgataagc cttgccatcc aagacaatca attccccatc
    14461 aatggaatcc aaggttccca aaccaaggtc accatgttct agcaattctc ctactgtcat
    14521 agtaccacca taaaggccag ccatcaaggc tccaagagta ttgtattgaa ataatttcac
    14581 tggttcctgc acgttcttat ccattcactt tcttgtctgt tattctataa atcttactcc
    14641 taagtatacc acatttgccc ctagatgtga acgagagaaa cgctctagac attgccaaga
    14701 aggaaaaaaa agggtacaat gtaacaaaat caagggaggt ctggaatgaa gaaacaaagc
    14761 aagtacaaag aggtcgtttc ctatctgaaa aatggtatcg agtctgaacg atttccgacg
    14821 ggtagtcgcc tgccttctat ccgtcaactg agccttgact ttcactgcag caaggacacc
    14881 attcaacgag ccctgctgga attacggcac gaacaatacc tctatgccaa gcctcagagt
    14941 ggctactatg tattagaaca agggcaacat caagacctag aaatcgaggt taccgacgaa
    15001 catgccagtg cctatgacga tttccgactc tgtgtcaatg aaaccttgat tggccgagaa
    15061 aactacctct tcaactacta tgacaatcaa gaaggattag aagacctaag acagtccatt
    15121 cacaaactcc tctttgagca agctctctac tgcaaggcta accaactagt actgacttct
    15181 ggaacccaac aagccttgtt tatcctctct caaatatcct ttcctagaca agccaaggaa
    15241 atcttggtgg aacagccaac ctaccatcgg atgaatcgcc tcttgattgc acaggggctg
    15301 gactatcaaa cgattgaacg aggcattgat gggattgact tggaggagct ggaaggccac
    15361 ttcaaaacag gaaaaattaa atttttctac accattcccc gatttcacta tcctctggga
    15421 cattcctatt ctgagcagga caaacgatct attcttaact tagctgccaa gtatgatgtc
    15481 tatatcgtag aggacgatta tctgggtgat ttggactcca agaagggcca aaccttccac
    15541 tatcttgata cagaggagcg tgtcatttat atcaagtcct tctcgaccag cctttttcct
    15601 gcccttcgta ttacagcact cattcttcca aatgctatca aagaagcatt tgtggcctac
    15661 aaaaatatcc tagactacga cagcaacctc attatgcaaa aggccctgtc actctatatt
    15721 gacagtcaat tgtttgaaaa aaatcgtttg gctcgcttga ccaatcatga atcttaccaa
    15781 aaacaaatcg aggaaaggat aactaaaaca ccttgtcccc ttcctcatta tcccctacac
    15841 gatggtttat tgctagacct gagacagtat cctaaaatcg ccagtctcaa acacagtcaa
    15901 ctgggcttgg acttctttga agaggcctat ttaagcacct gtccttatca atttgccaag
    15961 gtgtccctag acaatctgga aaatgtttta aactatttaa aagcagaatt ggaatgacat
    16021 ccaactctgc tttttcttat tcttcaactt cagttagact tgctgctttt tcaagatagc
    16081 tttggtaagt tccttcatcc ttaagttttt ggatgacctt gtctaccact tctttcaaat
    16141 catcactatt ttttctaaga gcaacggcat tggcgtcgcc gtccttcatc ttcaagctga
    16201 cagttgcgac agctaagcca gcgtttttag cagcataact aagtgcaaca ggctcatcca
    16261 tatgaacagc atctactttt ccagcctgca attcattgac tgcttcaccc atattagtta
    16321 gggaagttaa ttgagctttt ggcaattgtt ccttgaccat tgattctgga acagtccctt
    16381 tttgggctgc aatattagca ctttctaggc tagttaaatc cttgtatttt tccacatcag
    16441 ccttacgaac caagaaacta atcttgtttt catagtatgg gattgaaaaa tcaaagactt
    16501 cttttctctc gtcagtagca ctaattcctg caactgctag gtcagcctta ccagtttgaa
    16561 gactggtcaa aacattgtca aaactcatgc ttgagatttc caacttaacc ccaagttcat
    16621 cagcgatagc ctgagccatg tcgatgtctg caccgactac ctgattcttt ccgtcaacca
    16681 atgattgaaa ttcaaagggt gcatagtcag gactggttgc cacaactaat ttcccctttt
    16741 gcttaatggc ctcaacagct gactgagaac cattagaacc tgactggcaa gctactaaga
    16801 agaaacttgc aagaaaatta cataaaacaa atatccattt ttttgatttc ataaataaac
    16861 aacctctctg ttaattttgt ataattataa cgatatccaa gttacttgtc aagtgttttt
    16921 taaattttta tctcaaaaat attttttcgt tcaaaaaaag gagccatcag ttgatttcaa
    16981 gctccttttt atacagaatt aaactatttt atagttcgac aatcttacct gtttcaaagt
    17041 agacaaccca ttcacagata tttttagcat agtcaccgat acgctccaag taggaaataa
    17101 cttggaaata atcacgaccc gtaacaatgg cttctggatt tttcttaatc tcttcagtcg
    17161 caaggtcacg gatagtttca aaatagtggt taatttgctc atccatggag gccacccggt
    17221 atgcgtcgtc aacagaacca ttaagataaa gatcaagtgc tgcttcaaca acgcttttaa
    17281 cttcacgtcc cattttttta atttcttcct ctacagctgg gatgcgctct tcccccttca
    17341 tacggatggt tgcctgggca atggctacag cgtgatcccc catacgctcc acatctgata
    17401 cagccttaag gacagtcaag actgtacgca aatcttgaga gactggttgt tggagtgcga
    17461 tcatttcaaa tgatttcttt tccagtttca cttcgtattc atttacttct gcatcatctt
    17521 cgatgacctc ttttgccagg tcacggtcat gcgtgacaaa agcacgtacc gtacgattga
    17581 tttgtgagag cacttcttgt cccatagcgt agaactggtt atgtaatttc tctaaatctt
    17641 cttcaaattg agatcgtaac atctttcatc tccttatcca aattttcctg taatatagtc
    17701 ttccgtttcc ttgtgttggg gatcaaggaa catctgcttg gtatcattaa attcaatcaa
    17761 atctccatct aggaaaaatc ctgtcttatc agagatacgt gaagcttgct gcatggaacg
    17821 ggttaccaga agcatggtgt acttgtcttt tagaccatac aaggtttcct caattttacc
    17881 agctgaaatc ggatccaaag ccgaagttgg ctcatccaag aggatgattt taggactagt
    17941 tgccaagaca cgggccacgc agacacgctg ctgttgacca cctgacaatc caatagctga
    18001 atcatataga cgatccttga cctcatccca gatagaggca ccttgcaagg ctttttctac
    18061 ggcttcatcc agaacctgct tatccttaat tccattgata cgaagcccgt agacaacatt
    18121 ctcatagata gtcataggga aaggattagg ttgttggaaa accattccga tttccttacg
    18181 taattcaacc gtatctgtac gcggactgta gatgttgtga ccattgtaca ccacggatcc
    18241 agttgtggtc acctctggat tgagatctcc catgcggttg agagacttga ggagggttga
    18301 cttccctgat ccagatggac caatcaaggc tgtaatttcc ttaggttgga aagataggga
    18361 aacactattc aaagccttct ttttattata ataaacggac aggtctgata cctgtaaaat
    18421 cgcatctgtc atacggtttc ctttctaacc aaagtgacca gatacatagt cattggtgga
    18481 ctgtagcttg gcattttgga aaatagttgc agtcttgtca tactcaatca aatcacccaa
    18541 gtaaaagaag cctgtatagt cacttgcacg agcagcctgc tgcatattat gcgttacaat
    18601 gatgatggta aagtttttct tgagctcaaa catggtctct tctagttgca tggtcgcaat
    18661 cggatccaag gctgaggctg gctcatccat taagaggata tctggcttaa cagagatggc
    18721 acgagcgata cagagacgtt gttgctgacc acctgataag gtcaaggctg acttgtggag
    18781 atcgtcttta acctgatccc agagggcagc ctgacgaagg gaggtttcta cgatttcatc
    18841 taggacttgc ttatccttaa ctccagcacg ttcatgcgca aaggtaatat tacggtaaat
    18901 tgacttagca aatggattgg ggcgttgaaa aaccattcca atgtgtttac gcatttcata
    18961 aacgttgatt tctggacggt tgacatcaat tccacgatag agaatctgcc cagttacttt
    19021 agcaatatca atggtatcat tcatgcgatt gagactgcgt aagtaggtag atttccccga
    19081 tcccgacgga ccaatcaaag ctgtaatttt atttctttca aattgcatat caatcccctt
    19141 aatggattca tttttaccat agtaaacatg gacatcctta gtagaaaggg ctactttttc
    19201 ttcaggaaag gtaaggatat gcttctcatc ccagttatat gttgacatgg cttctccttt
    19261 aggcagcggt taatttcttg tgtagatagc ttccgaactt acgagctcca aagttaaaaa
    19321 tcaggataaa gatcaggagc acagcggcag aacctgctga tacaatggtt ccatctggaa
    19381 tagtgccttc actattgact ttccagatat ggacagccaa ggtttctgct tgacggaaga
    19441 tagagatggg gctagtcaca ctgaggatat tccagttaga ccagtcaaga gctggcgccg
    19501 attgccctgc tgtatagatc agagctgcag cttcgccaaa gatacgacca gatgccaaga
    19561 cgacacccgt tacaatacct ggaagcgctt ctggaataac aacatgaacc actgtctccc
    19621 agcgagaaat cccaagagcc agaccagcct cacgttgggt atggtgaacg tgtttcaaac
    19681 tatcctctac attacgcgtc atctgaggca agttaaagac tgtcaaggcc aaggcacctg
    19741 aaatgattga aaatccatac tcaaactgga ctacaaagat caagtaacca aagagaccca
    19801 ccaccactga tggtaaagag gacaagattt caatacaagt ccgcacaaag ttggtaacag
    19861 gacctttttt agcatattca gccaagtaaa tcccagctcc catagaaaga ggtacagaaa
    19921 taatcaaggt aatgaccaat aggaaaaagg aattgtaaag ctgaatgcca atcccaccac
    19981 ctgcttgaaa agcagaagac cttccagtca agaaagacca agagatatgg ggcaagcccc
    20041 gaaccaagat atagagaatt aaggaagcca agattgtcac aatgatgcta gcaatcgtat
    20101 agaggacagc tgttgcaagt ttatctaatt tcttagcgcg cataattttt ctttcctctt
    20161 tctttcgtaa tcaatttaat cacactgtta aaaactaaac tcatcaagag cagtaccaag
    20221 gccagtgacc agagaacatt attatttaca gttcccatga cagtgttccc aattcccata
    20281 gttaatatag aagttaaagt tgcagctggt gtggtcaagg aagttgggat aacagctgag
    20341 tttccgacaa ccatctggat agctaaagcc tcaccaaagg cacgcgccat cccaaagacc
    20401 actgcagtga aaataccaga acgggccgcc ttcaagatca cacgccagat agtctgccag
    20461 cgagtggctc ccatagcgaa actggcttca cgataataac gaggaaccgc acgcaagcta
    20521 tccgttgtca taaaggttac ggtcggcaaa atcatgacaa agaggacgga aatccctgac
    20581 aaaatcccaa aaccagtccc accaaagaca ctgcgaacaa agggaacgac gacttgcaag
    20641 ccaataaatc cgtacactac tgaaggaatc ccaaccagga gttcaatagc tggttgcaaa
    20701 atcttcgccc cttttggtga tacttcggtc ataaaaactg ctgcaccaat agcaaagggg
    20761 gttgcgataa gggctgagag aatggtaacg ataaaggaac ccaaaatcat aggaagggca
    20821 ccaaattctt tactagaagg attccaagtt cctcccaaaa gaaagtcaaa gatattcaca
    20881 ccattgacaa agaaggtcga caagcctttt tgcgctacga aaaccaaaat catggccaca
    20941 aggatgacta tcaaagaaag acaggcaaag gtcaaacctt ttcctaattt ctccagacga
    21001 gaattctttg atggaagcaa cattttctta gctaattctt cttgattcat tattgtctcc
    21061 cttccaacac tgtcacagtt ccggcagcat ctttttcaac cttcatttcc ttaatcggaa
    21121 tatacttcaa tcctttgaca atcccttctt gggtctcatc cgagagaaca aaattgagaa
    21181 attctgcagc caactcattg ggctgcccca atgtatacat atgctcataa gaccacaagg
    21241 gccaattatt gctacttata ttttctggac ttaagtcata gccattcaac ttcatgcttt
    21301 tgaccgaatc atctatatag gtaagagata aataagagat agctcctgga ctttttgata
    21361 cgattgattt taccgctcca tttgaatcct gctcctgact ttgcatggca gactgacctt
    21421 ccataatgac agtatcaaag gtagcacgag agccagagcc ggctgcccga ttgataacag
    21481 agatgggtaa gtccttacca ccaacctctt tccaattggt tacctcacct atgaagattt
    21541 gacgaagttg ctctgtcgtt aggttatcaa catcaacctc cttattgaca atcagagcca
    21601 agccagctac cgcgaccttg tggtcaacaa gagcagaagc atcaattccg tctttttcct
    21661 cagcaaatac atctgagttt cctatatcaa ctgccccaga ctgaacctgg gacaagcctg
    21721 taccagaacc tcccccttgg acattgaccg tttttccaac atgaatcgtg ccaaattcat
    21781 ctgccgctac ttcaaccaag ggttgcaagg cagttgagcc aacagccgtt atggattctc
    21841 cacgatcaat ccagctagca cagcctacta aacaagccgt cagccaaaaa gcgataagag
    21901 acagagcaag cttttttctt tttttcactg tttttctcct cgaaaataat tatgaatact
    21961 gtgaattttt taagtagttc tttatgagtt gacgcatgaa ttcttaccaa atttctgcgc
    22021 aattgattat ttatataata taggctatat tactctttcc taacctcctt ttttcatatg
    22081 tggataaaat ctcttgtcta tcccttcccc cattgtcacc cattataatc atttcgtgtc
    22141 tctttttccc ctttttaatg caagggaaat tactctcctc agatgataat ccaaaagcta
    22201 gaaaggtatc tcaaacctct ctactctccc agactagttt acaactaaaa ggaaaagatt
    22261 ctattttatg agaaatctag tttacaagcg gtaagaacgc taataactaa acttcttgta
    22321 ctctttgaaa atctcttcaa accagtgttt tgagctatct atggctagct tcctagtttg
    22381 ctctttgatt ttcatcgagt agtaaaacta catgtgatgg caatcaagat atcaagaatc
    22441 atcctactaa aaaaatccat actttcacta taacatagaa taagatattt gactagcatt
    22501 ttcatttgaa tctgaggcct tttggaaaat aatttttcaa aacatttcca gtaacctttg
    22561 caaagcccaa gccattgcct ttaaccaaaa cttggtacca accatttggc agactttctg
    22621 ccagctgaac ggtttctcca gccgcatact tgacaaacgc ttcttggcca atttcaaccg
    22681 actgttcgac ctgactcggt ttcaaggcta aaccaagagc gaaactgggc tcaaagcgtt
    22741 tcttcttaaa agtgcccaga tgcagtccat tgcgagcaat cttgagcttc cctaaatctg
    22801 gcaaaagttc tggcaagaga taaagctggt ctccaaaagt ctgcaagata cccggtagat
    22861 tgaccttcaa atggttttgg gcaaattcct gccacaaggc aacttgttca cggctgaggt
    22921 tactcttact tgccttaaat ttaggagctg gattgttacc cttaaactgt agatgggcaa
    22981 caaactgacc ctctccctta aactgatgag ggtacatccg agccgtttct ggcaggtcaa
    23041 taccagctac cattccattg atatgctcta ctggcaacaa gtcaaaatca tactcttcca
    23101 gcaaccaatt gacaatctct tcgttttcct cgggtgccca ggtacaggtc gaataaacca
    23161 gacgaccacc ttcagctaac atggtcactg catcctccag aatttctctt tgcaagctag
    23221 cacattgact cggataatct aagctccaat agtccatagc atcaggttgc ttacgaaaca
    23281 ttccttcacc agagcaaggg gcatcaagaa cgattaagtc aaaatagcct ttaaagacct
    23341 tgaccaagcg gtcggcagat tcattggtca ccacgacatt tgtcgctcca aaacgctcca
    23401 tgttttcaac caaaatctta gcccgtttgc ttgaaatttc attggaaaca agtagcccct
    23461 cacctgctag ataggctgcc agttgagttg atttgccccc cggtgcagca gccaagtcca
    23521 agaccttcat accaggactg ggttgggcta cttgagccac catttgagca gcaggttctt
    23581 gcgaataaac taaacctgta gcatgctcag gcgatttccc tgaaaccttc ccatagtggc
    23641 cccaaggggt ttgagtaatg gcatcagaaa aggaaagttg ctcttctttt aagggattga
    23701 cccgaaaggc cgaaaccgct tcctcctcaa aagaggcaag aaaatctctt gcctcatctc
    23761 ctagtatctc tttatatttt tcaacaaatc cttctggaaa ttgcatttaa gttcttttcc
    23821 tttcgtaaat ataggactga atttcctcct gcatctcaag aggcaccatc atgaccggct
    23881 gtctggtttg aaaatcagga gcttcaccaa aaagggtcac aacccgatag cccagacttt
    23941 cccctaaaat actagctgcg gcataatccc atggttgcag ataagtgaga taggtcaaca
    24001 aacgccctga caaaatcttg gcaaaactaa tggccgcact tccatagaca cgaacaccaa
    24061 gaaccgatcg gctcaaatca gccagccccc attcattggt ttccagcata ccactattcc
    24121 ctgcaatgag aaaatctcca agtggtttag ttttaaaagg agctaggggc ctatcattta
    24181 gacaaactgg aaattcccca ccaccgtggt aacaatcccc tttgaccaca tcataaatca
    24241 gaccaaactg tccctgacca ttttcaaaat aagccatcat aacagcaaaa tcttcctgct
    24301 gggctacaaa attattggta ccatcaatgg gatcaatgac ccaaaccttg ccctcttgaa
    24361 ccgaggctcg cagacaacct tcttcagcac aaatcttatc ctcaggataa cgggacaaaa
    24421 tctcaccaac caagagttcc tgaacttctt tgtccagtct ggtcaccaaa tctgttgaag
    24481 aggacttggt ttcaacacgc aagtcttcct gcatatggtc aagaatgtac tggcctgctt
    24541 tcttaacaag ctctttagca aattcaaatt tactttccaa gagaaatctt tccttcccct
    24601 ttttctttgg cagcctgaac tgctcggtaa agtgaatagc cactaaccgt ttcaaattct
    24661 cgtcccaaac gtttctcttc gctcttgctt ggcacgaccg ctttgaatcc cttataggag
    24721 tccagtaact ttttagcctc tactttgcct tcataggcag cttcaacatc attaaaaaaa
    24781 gaaagcactg aagcaagttc ttcagtgctc cacgacaaat ctagtgggta actatactgt
    24841 ttgttcatta actaatacca gctctcattc ttgcttcttt tagttcttgc ttacgataac
    24901 tacgagggag aaaagcacga atctcatctt cattaaaacc gatttgcata cgcttggcat
    24961 caataataat tggacgacgc aaaagactag gatactgctc aatcaaatga agcaattccg
    25021 ataccgaaat actctctaca tcaatattca atttttgaaa aatttttgaa cgagttgaaa
    25081 tgatgtcatc agtaccattt tcggtcaagg aaaggatgtg ttgcaattct tttcttgtta
    25141 aaggactggt cataatattg tgttccacaa agggaacctt atgtttttct aaccaggcct
    25201 tagccttacg acatgatgta cagctcggtg atagaaatag tgtaatcatg cttttctctt
    25261 cttatctata ctttgctact tctattatac aaaaaaataa ggcgcttgac tagggatttt
    25321 tagaaaaaaa gcctattttt tcaagaaaaa taggctgttt gcgaacgatt gacacaattg
    25381 gatttggtta attcactctt aacgatggtt ttaaacgata tatattttta tatatgcaaa
    25441 ttaaaaacat ctttcatttc acttcctacg acttttcaga cacagatacc caaagaagct
    25501 ttcatagagg gcaaaaaaga ggaggaaggc atgaagaaag aaggtctctg gcaaaatcat
    25561 aataacagga tccttggctg gatcaaaaag ccaggtatca tctcccacaa agagaatttg
    25621 atggaaaaga gtaaagaatt ggtcaaaacc aatcaaaact cccccaagtc caatcatcac
    25681 aggtaagact actagagtca ggagactttt tcgataaaga gacaaaaagt cctttttcac
    25741 aatcctattg acaaagacat agaaacttgg cagtgtcact agagctacta gctgaaccaa
    25801 atgaaagaga ttcttgacca ctgcgaaatg gtgcagacca gctgctgacg aacgaaaatc
    25861 aggcatctgt aagacctgac taaaaggatt ggtcagataa ttcatcaaga tatgaaaatt
    25921 gtattgaatg gtttctggtt ttagatagac tcgattcgtt aagtttagcc actgaatctc
    25981 cataggatag aaaatccaag ccagataaat ggtcagaagg attgagaggg agaggagaaa
    26041 gagcatagag ccccaaaagg tcaatttagt tttcatcaaa atcccactcc gcaaggctag
    26101 aaaccacatg tgtcggtgcg attggcaggc cagctacttc ttctgccttg gtaaaacctg
    26161 tcgtcaccaa gagtgttgga atgccattgt caatcccagc ccggatatca gtcaaataat
    26221 tgtccccaac catgattaac tcttcacgtt ccaaacctaa gtgctcaacc gccttgtcca
    26281 taatgatggc atttggtttt ccgatataaa ccggcttcac tcgtgtcgct acttcaagca
    26341 gcgtaatcag tgagccagca cctggcaaaa gaccacgttc cgtcgggatg ttgaggtcag
    26401 gattggttcc gataaaatgg gcaccctttt gaatagcaag agttgctgtg gcaaattttt
    26461 catagtcgac ttgccaatcc agaccaacta ccacgtaggc agggttttcc ttgtcttcca
    26521 cataaccagc cgccttgatg gcttccttga gtcctgcttc tccgacgaca tagacggtct
    26581 tttcaagccc caaatcattc atatagtcga tggttgccaa agtcgctgtg tagacagtcg
    26641 atagaggcgt atcgatatta aaattctgag ccaacatctc cttaacactc tctggagtgc
    26701 gggttgtatt gttggttaca aagagatagg gaatgtcccg cttttgcaat tcatgaacaa
    26761 aagtctctcc agcagggatt cggtctttcc ccttataaat ggttccgtct aaatcgatta
    26821 aatagccttt atatttcatc tatttctccc taaacctttt ttatttcttg ccaagtaatg
    26881 attgcttggg cattgataac cccatcactt gtaatttcat gcttgctttc cagtccagtc
    26941 cgttcaacag ccgatgtaat caccccacct ggtcgaactt ccttgacata cttgaggttg
    27001 attttcttgg gaatatagtg ggtcaaaaaa tccgctccca tgacctcaaa aatccagtcc
    27061 aagtatttac tgttattgac atgaccattc atatccaagt cgtaaaaacg aacatggtaa
    27121 tccttgctga tcggctcttc caaggattca tacttcggtc cacggataag ttttttatca
    27181 aaatcagact ggtaaggagc cacaatctca ggttcaacag catggacttt tcgactgtcg
    27241 cggtccatga gaacaaaggt cgccatcata tggatgagct cctgccccgc ttcattataa
    27301 atagtaaagc gacggtagca aaaaagtcga ttgtagctca aggcttccgt ttcgatggta
    27361 atttcttccg caaaacgagg caaacgaacc acctcaatat catattctgc gataatccag
    27421 accagattat agtcttccaa aatggcctta tcactaactc ccagttcaat cgattgcatc
    27481 cctgaaactt gcagtgacag caaaatcaca tctggaagtt tgatatgacc gttcatatca
    27541 gccatatcaa aaggaatttt cattttcatt tgataagtta agcccatgat cctactccaa
    27601 aataaatcgt tctgctacag tatctcccaa aaagagacct ctctttgtca tgcgaacgtg
    27661 gtcaccctca atctgcatga ggccttgttg aaccaaatct ctgacaattt ctccataaag
    27721 tccagcaaaa gactgtccaa atttttcctc aaatcgcgcc atggaaaccc cggatttctt
    27781 gcggagtccc aagaacattt cttcttccat ttgctccttt tgactcaggt gatcttctgt
    27841 aatacaagca ttgccttcct caaccgcact gagataatga cgaatgggac catgattttt
    27901 atagcgtact ccattgacat aaccagatgc ccctgcacca ataccatagt attcagcatt
    27961 gtcccagtac atgagattat gacgactttc aaaaccgggt ttggagaaat tagaaatctc
    28021 ataatgctca aaacccgctc gctccagctc tgcaatgatg tactcaaaca tctccgcttc
    28081 tagttcctcc ttaggcagag gcaatttccc acgtcgcatc cggttcataa agaccgtatg
    28141 gttttctaaa atcaaactat acaaactcat gtggggaata tccaatccaa tggctttagc
    28201 cacattttcc tttacttgct ccatggtctg accaggcaga gcataaatca aatcaatgga
    28261 gatgttgtca aaaccagcca gtttcaggcg atcgatattt tcataaatat ccttctccaa
    28321 atgactgcgc ccaatctttt tcaacatctt atcatcaaag gtctggacac ctagcgaaac
    28381 acgattgaca gccgaatttt tcaaaacagc tatcttatcc gcatccaaat cgcctggatt
    28441 ggcttcaatg gtcaactctt ccaagacaga caaatccaag tttttagtca agccattcag
    28501 taacacctcc agttgcggag ccgacagggc tgtcggtgtt ccaccaccga tataaagggt
    28561 tgacaacttt tcaatatcat aagaacgaaa ctcttccagc agatgctcta aatagctgtc
    28621 gactggctga tttttgatga agacctttga aaaatcacaa taataacaaa tctgggtaca
    28681 aaatgggatg tgcacatagg ctgacgttgg ttttttctgc atagtaatta ttataccaca
    28741 aagactagat tccagataaa aatcaccatc cccagataca tagtccgtcc ggagatggtg
    28801 atggtttatt cttctgttat atgaatcaca atctcttctg agtcatcaag agcttcggct
    28861 ttttcttgcc attgttcctt gagattattt aattgatttt ttgatgcttc tgtcgcttga
    28921 aaagcatagg atttagcttg agcaagtata ctgtccacag tgatttcacc tgactcaacc
    28981 tgttcttttg ttttcagaac aaaatctgta gcctgctcct taacttctgt cagtttttca
    29041 cagacttgct ccttggcata ctccggatct tctctcaaat catctagaaa atcttgagcc
    29101 tgactgcaaa cttgtttgcc cttatcactt gttaaaaaca aggcaagagc tgcacctgaa
    29161 accgttccta aaaggattga ggataattta cccataagga ttctcctttt ttattttttg
    29221 aaaaatttac ttgcaagacg aagagctgac agacttgcac cagtcttgag tgtttttgaa
    29281 ccagctgatg aagctttctt gctcaagaca cgcgcatggt cattgaggtc tgaaacagat
    29341 agagataaat ctgcaacagc actgaagagt ggatcaatcg tagccacctt gacattgata
    29401 tcatctgcca agacattgac cttagccaac aactcattgg tgtgatgcaa ggtcacatcc
    29461 acatctgaag tcaaggtttt aatcgtcttt tctgtttcat cgatgacacg accaagcttt
    29521 tgtacagtaa tgatcagata gaccaaaaag acaatcaaag ctagggcaac aagaatatat
    29581 gcaacttcta acatttagtt ttcctcctct gtaatatagt aaggggcctt ctttcgattt
    29641 tgataaataa cgatcattat accgagaccg ataaggacaa ctgacagcca ttgggacact
    29701 cgaagtccga agaacatgag actatctgtt cgcatacctt cgatgaccat acgaccgaaa
    29761 ccataccaaa tcaagtaaaa ggccgtgata tgacctcgtc tgagactctt ccatttccgt
    29821 ctaaaaatca gaatcaaggc aaagccaagc agattccata gagactcata aaggaaagtc
    29881 ggttgacggt agctcccctc aatatacatc tggtcacgga taaagccagg tagataatcc
    29941 agattatcca ctgttgcacc ataagcttct tggttaaaga aattacccca acgccccaaa
    30001 ctttgagcaa tcataacgct aggcgccgca atatctagaa aatcccaagt attgatgagt
    30061 ttacggtcag caaagatata gagcacaaga gccccagtta tcaaaccacc gtaaatggcc
    30121 aaaccaccat tccaaatggc aaaaatctct cctaaattct gactatagta atcaaatcgg
    30181 aaaataacat agtagagacg agctcctaaa atagccaagg gaaaggctac taagataaaa
    30241 tctaaaatat cgtctggtat gatcttcttt ctaggtgctt ctttcatggt caaataaacc
    30301 gcaagaatca agcctgtcac aatacataag gcataccaac gaatggctag gggtcctagt
    30361 tgaatagcaa ttggatcaag cattttgcac ctcatttcga gcgatgagac ttgtcagtcg
    30421 ttcgtcgaac aaacgggtcg catcaaagcc catttccttg gcacgataat tcatggcagc
    30481 tgcctcaatc acaacagaga tattacgacc tgttttaact ggaatacgaa tacgaggaat
    30541 ggctacgcca gaaacttcaa gttcctctgc attgtttcca agacgatcaa aggtcttatg
    30601 cgtatcgtaa ttttccaaat agacagcaag ctgaacctgt gaagaatcct tgacagcact
    30661 cgcaccgtag agactcataa catcgataat accaacccca cgaatttcaa tcaagtgttt
    30721 caaaatttca gctggttcac cccagagagt aatctcatcc ttggcaaaga tatcgacacg
    30781 gtcatcggct accaaacggt gaccacgttt gacaagctca agacctgtct cactcttacc
    30841 aattccacta tctccctgaa tcaagacgcc catcccataa atatccatca agacaccgtg
    30901 cacactggta cgttctgcca aacgagaatc cagatagcta gataactctc cagataaacg
    30961 actggtagct gcacggctgg ttaaaatagc aatcttacat tctctagcag cctttaacat
    31021 ctcctctgga accaccaaac cacgggcaac aatgactgct ggtgtctcag gtagaaacat
    31081 ttttttcaaa acttcataac ggctgttgga aggcatgctg atcagataag accactcctt
    31141 catccccaaa agttgaatcc gctctggtgt atagtagtca aaatagcctg tcatttcaag
    31201 accaggtcgc gtaatatccg ctatattgat ttccttttca agcaattctg gttcaccata
    31261 gacaatgtct agtctaagct tttcaatcac ttcttttact aaaaccgaca tcatttcctc
    31321 cttcaaatga gttcttctct ctattatatc aaattgtagg aggaagtttc agatttttgc
    31381 aggctttgga gtatttattt aaaagaaaaa ggactagatt tctctagcct ttcatttcaa
    31441 ttagaatttt ttacgtttac gagctacttc tgatttacgt ttacgtttta cagaaggttt
    31501 ttcatagaat tcacgtttgc gtgtttcttg aagagtacca gctttagtaa ccgcacgttt
    31561 gaaacggcga agtgcgtcgt caagagattc attcttacgt actactgttt tagacatttt
    31621 tttcactccc ttcaagttca atatctattt cattctacac gtaaaaggaa taattgtcaa
    31681 gcaaaaatgc ggtttaattt tgattttttc ttccatttat acaagagttg aaagcgttca
    31741 atagaacatt gcttttttat ttttcgagta agctaagcgc ttcagcatct gcgatgatgg
    31801 ttacatcagg gtgattttgg aggctacttg caggtaggtt ctcagtcact gggccagata
    31861 ctgttccggc aatggcttct gctttcgact caccgtaagc aaaaagaata atagacttgg
    31921 catccaaaat gtttttaatc cccattgaaa tagcttgggt tgggacgtct tcaatcttgg
    31981 caaagaagcg tgcattggct tcgatagtag actggtcaag ttctactaga tgcgtttgac
    32041 tgtcaaatgg agtaccaggc tcattaaagc cgatatgccc attgcgaccg attcccaaga
    32101 tttggaggtc aactggatgg tcagccaaaa tttggttgta gcgttcaact tcagcttcag
    32161 cattgtcctt aacaccacga ggcaagaaac tttctttaaa tggtttttgg ttgaacaagt
    32221 tttcttgcat gaagtaacga taagactgtg gattgtcccc atcaaggccc acatactcat
    32281 caaggttgac actggttaga tttgaaaaat caaggttact ctcaacaatt tccttgtaaa
    32341 attcaagtgg gctacttcct gtcgcaagtc ctagagtttg agctccatta gccaattttt
    32401 ccttcaaaat ctcaaaagca acttttccac cttgaacttg gttttcaact ttaataactt
    32461 tcatcttttc atcctccatt tctgtctata tttattatat ggtatagacc aatttttgtc
    32521 aagtgaaaac gatttaattt ctaaaaaaag agcgtccaaa cttgaaacgt gttataatgg
    32581 ataggattag aacttagaaa gaatgaacag atatatgaat acagctgatt ttgatttcca
    32641 cttgccagag gaattgattg cccaaacgcc ccttgaaaaa cgtgatgcct ccaaactcct
    32701 catcgtcaac cgtgagacag gagaaatgca agataaacac ttccactcta ttattgatat
    32761 gctggaacct ggtgatgccc ttgtcatgaa cgacacccga gttctccctg cccgcctcta
    32821 tggtcaaaaa gtggagacag gaagtcatgt ggaacttctc ctccttaaga acactagtgg
    32881 agacgagtgg gaagttctgg ctaaacctgc caaacgcctc aaggtcggta ctcgtatcag
    32941 ctttggtgat ggccgcctca gcgctgtcgt tacagaagaa ttgacccacg ggggacgcat
    33001 tgtccgcttt gaataccaaa gaattttcct agaagtcttg gaaagtctgg gagaaatgcc
    33061 tctgccacct tatatccacg aaaaattaga tgaccgtgaa cgttatcaaa ccgtctacgc
    33121 caaggaaagc ggctctgctg cagcaccgac tgctggtctt cacttcacca aagaactgct
    33181 ggcagaaatc caagctaagg gtgtccatct agtctatctg actctccatg tcggactcgg
    33241 aacctttaga cctgtttctg tggataatct ggacgaacac gaaatgcact cagagttcta
    33301 tcaactttct gaggaagctg cggccaccct tcgctctgtc aaagaaaatg gtggtcgtgt
    33361 catcgctgtc ggaaccactt ctatccgtac cttggaaact attggttcca agtttgatgg
    33421 gcaaatccaa gcagattctg gttggaccaa tatctttatc aaacctgggt atgagtggaa
    33481 ggtcgtggat gccttctcaa ccaacttcca cctgccaaaa tcaactctgg tcatgttggt
    33541 ttctgccttt gcaggccgtg aattagtctt agatgcctac caccattcca tccaagaaca
    33601 ctaccgcttc ttcagttttg gtgacgccat gtttatttat tgagaaagaa tttctctaaa
    33661 tcttctaata ccaataaatc gctaagatat tatttcaaag aacatctaca attgaaactc
    33721 tagctagctg tagaagacgc ctagtacatt gaaattaaaa tgcaagacac atgagtagca
    33781 ccatatttgt tgctcttatc tgtcctcgca agagactatt atgagttatt tcagaatcat
    33841 tcactacttt gaccctgact ctccttagtc tcaaaatcaa agacttatac tcttcaaaaa
    33901 tctcttcaaa ccgcgtcaac gtcatcttgg attatatatg tgactgactt cgtcagttct
    33961 atctacaacc tcaaagcagt actttgagca acctgcgact agttttctag tttgctcttt
    34021 gattttcatt gagtattaaa caaaaagtga acaaagctga attctaatgt acagaagact
    34081 aggcttgttc acttttttat agtcgctata agatgacctt atctatagct ttttatatat
    34141 aattatatat tcagacatac tattatcaat tttgtcgcag ggaggaatct gttaacgcac
    34201 ccattcacca ttatcattga ctctatagcc atctatactt gtattgaccg ctaactcacc
    34261 cgatgtattt acataatacc atttaccacc aacttggaac cattgattga ctttcataga
    34321 accgttgctg ttgaggtagt accatgaacc attaacttgt acccaacctg ttgccatgga
    34381 accatcagta ttataaaaat accacatacc attttcttgt ttccagcctg ttgttggagc
    34441 aactgcttta gctggttcta ctgctacatc tgttccttgg ttagatgtaa cagatacagg
    34501 atacgaagga atagatgatt gctcaggaac aacaactttt tcaggttctc tcgtccctct
    34561 ccttatacgt ctttttacca tctctttagt aatttgacga gaagtagttt cttcaattgt
    34621 tccatcacgt tcatctacag catagattgt agtaagagta attttaccaa tttctcctac
    34681 ttcttgacct ttatcaagag ttgggtcatc gagatattct gtttcgattg gaatttcttg
    34741 gacaagaact tggggcttgg ttcctttttt aacaactctt gtttgagagt cttctttttg
    34801 acttaaagta ctctcagtta cttgtccact ctttccatct acatcataag ttatcgttgt
    34861 aactgttttc ccattctttc ctagagtaat ctcttgctcc tgtcctgcag aaaggtcatt
    34921 gtctgcttca tatttagtag taaatggaac aagaacttct tcaaccttgc ttttagctgg
    34981 aactttgata actgtatccg tggcttcttt tctatcaaca gtaacctttt cggtaacatg
    35041 accagtctct ggattaacat cgtaggtcct tgtcgtagtt acatagccat cctctccatc
    35101 aattgtaaca ggattttcac tacggtcttt tgtttcatct ttttcataac gaattcgcgt
    35161 acttgaaatt ttcttggtta ctaccttagg tttagtcgct acttttacaa taatactccc
    35221 attgtcagcg tcatcatact ctattccctc ttctttatct ctagtatcat ctctgacata
    35281 ttgaatccca tcagcagcat gaacaaaact tgtattcaga ttcctcctaa aaataaagtt
    35341 agcccgatta ccgcagaacc aaaaatcttt ccgagtttac gtaatgcata gcgcttatta
    35401 gtattagatt ttgccattac atcctacttc tagtatagca tcttttctat caaacgttaa
    35461 acagtatact ttatatatca aatagactta gaatgatata ttgattattg aactaacact
    35521 ttaactatat cgtaatcaat ctcatatata aaggattgca gacatcttat ctaaatacat
    35581 gctaatatat ttagatacaa acattccaac ttgataattt tcactcatct ttcatcattc
    35641 cttatacaac tatgcagtat aaatagaata gttttctcat cagaatgaga ctattttaat
    35701 attagataat ttgatagaaa aaaacataaa taaaattggt tgaatttaat tccctaactc
    35761 gatagaagat tctattttaa atttaacaac agctaggatt acactataaa aagcacatta
    35821 ccacctgttc gactggagaa tctagtcgat tagctccctc ttagttacaa gcttaaagcg
    35881 attttttaac taatactaaa ccagattaaa gaaggaacta gaacatctgt cttgtactaa
    35941 tcaatatgct attttaaaat tgcaaataaa cttggaatgc taattattat atttacaata
    36001 aatagttgac ttcgatagaa acagaaaata gaattcaaca ttcttcccta cttataccat
    36061 aataaataag atccgcaaga tagccttttc tctctacacc attaacaatg gtttgtagat
    36121 aggacattcc agccttttcc atgacacgac ctgaagctgg gttgagactg gcataacgtg
    36181 ctctgacctt ttgaaaacct gcttgagtaa aacaaaagtc caagacagct ttcaaggcct
    36241 ctgtcatcat accatgtccc cagtaagcct tgcctagcac atagccgatt tcacagctta
    36301 aatcagcctc gtctatctta acgatgctga tatctcctat cacttgctct gggttttctt
    36361 ttagacaaat ggcccattta taatagttga gattagtata ggaagcaacc caattgcgaa
    36421 tcgagtttcg agtgatttcg acatcaggat ggggatccca ggtaacatag gtcagattct
    36481 cagcggatga agcccaattt tgaaacatgg cttctgcatc actctccaca aatcttctta
    36541 aaatcaaacg ttctgtctgt aatatttgcg taccgataga tttcatgatg ctacctcact
    36601 tccttatttc tatgctcgta gtaaaaagac tcaactcagt aacagctaga aaacgagttt
    36661 gtcttctaat ttcttgacgc agttgctgca tggtttgaaa tgcgctctag caatcttttt
    36721 caaacctagt catcgttgca ggggtaagac gatgaaatcg aactcttcga gttaccgaat
    36781 ctgcctcatt ttcttatttc taaactcggg gttcaagatc ccccggacct ttttactccc
    36841 aaaagtcatc aaatacggtg atgggtaagt ggcgtttgtg ttggcctttg tgccaccagt
    36901 tttcaatggt cgcttgagct tctgggctga ttgttttgcc ttctaggtaa tcgtcaatct
    36961 ctgcataggt gactccaagt gcgacttcgt cagctaggcc tggtttgtct tcttctaggt
    37021 ctgccgttgg gattttttca taaagggctg gctctgcgcc aagtttctgc aagagttgtt
    37081 ttccttggcg tttattgagg cggtaaagag ggagaatatc cgcaccgccg tcaccaaact
    37141 tggtaaagaa acctgtgata ttttccgcgg cgtggtctgt tccaatgacc gctccgctat
    37201 gggaaccagc aagggcatac tgagcaatca tacggcaacg tgccttgata ttccccttgt
    37261 tgaagtctga aacaggacta cctgtcgctt caactgcagc tgtcatggca tcagctgatt
    37321 ccttgatatt cacaaccaag ctgacatctg gctggatgaa ggctagggct ttttgagcat
    37381 ctgcttcatc agcttgcact ccgtatggca ggcggacagc gataaatttg tagctatcgt
    37441 ctcccgtttc agctcgcagt tcttccatag ctaattgcgc caaacgtcct gccaaggttg
    37501 aatcttgtcc cccagaaatc cctagtacaa aggtttttag gaagggatgt tttttcagat
    37561 atctttttaa gaaatcaata gaacgacgga tttcttcctg ggcatcaatc actggtttga
    37621 cacccagctc ttggataatc gtttcttgca aactcattct tcttctcctt caccaagggc
    37681 ttccttgcgc atcttatcaa tcaagtccat cttatcttgc catacatcac gcgccaaatc
    37741 cactggatag tgctgcggat tgagcacacg cttatactca tcccacaact tgtcaaattc
    37801 cttacgggca taatcctgaa tgtcagtcaa actaggcaag ttgtaaacta atataccttc
    37861 tttgaagata tccaccaaga gaggaacggc atcaaaatta cgaaccgtct tcttgatgta
    37921 tgtataggtc ggatggaaca tcttgatttc tgtcatgtcg ctaatatcca caccatcata
    37981 agtgatgtag tcaccttctg acttgccttt ttcacgactg gtaatgcgcc acacctgctt
    38041 cttacctggc gtcgacactt tttccgcatt attagacagc ttaatcgtat tgcgcatctg
    38101 accagtttca tcttcgattg caacaatctt gtaaaccgcc ccaagagctg gctggtcata
    38161 ggctgtaatc agcttggtac ccacgcccca gacatcaatc ttggcctttt gcatcttgag
    38221 gttgaggata gtattttcgt ccaaatcatt agaagcataa atcttagcct ctgtaaatcc
    38281 agcctcgtcc agttgctgac ggactttctt agaaatgtag gcaatatccc cagagtcaat
    38341 ccgcacaccc ataaagttaa tctgatcacc cagctcacgc gccacctgaa tggcagctgg
    38401 tacaccgatg cgaagggtgt cataggtatc cacaagaaag acacaatttt tgtgggtcgc
    38461 agcgtaagcc ttgaaagctt catagtcatt gccataaacc tgtaccaagg catgggcatg
    38521 ggttcccaaa acaggaatgt caaagagctt acccgcacgc acgttgctgg ttccattggc
    38581 gccaccaatc acagctgcgc gtgttcccca gatggccgca tccatttctt gagcccgacg
    38641 tgtcccaaac tccatcaagg gttcatcttc gataaccgaa cgaatacgag ctgccttcgt
    38701 cgccaccaag gtctggtagt tgacgatgtt caaaagagcc gtttcgacca actgacattg
    38761 ggctagaggt ccttccacct gcacaatcgg ttcattagca aaaaccaaat ccccttcttg
    38821 ggcagaacga acggtcaact ccaacttgaa attgcgaagg taatccaaga acgccccatg
    38881 ataaccaagc gactccaaat aggctatatc actatctgaa aaacgcaagt cttcaagata
    38941 gttcacaatt ctttccaaac ctgcaaaaac cgcatagccg ttcttaaaag gctgttggcg
    39001 gaaatacacc tcaaagaccg ccttcttatt gtgaatccct tggtcaaagt aaacctgcat
    39061 catgttgatc tggtacaagt ccgtgtgcaa tgtcaaacta tcatctggat acatactttt
    39121 cctacttcct tagctagaaa cccatgaaaa ttttcaagaa ctttcatgta ttccaataaa
    39181 ttagtactat tatatcacat tttagctgga ttgagaaaag agtaacaagc tattctccac
    39241 tctccaattc atccatatct tgttcaaatt ttttctgagc ccattcgcca tagctcttaa
    39301 gaccaagatt gccaataaag atccacggaa ggtaaatgat ataagtaatg acccaagcag
    39361 acaggtattt aaaattcaaa ggattgtgct gataaatttc tatgttgaat tgataattct
    39421 gcaacatcaa aagagccgta atagccaagg ttaggaaaaa acaacccaaa atcgtaaaat
    39481 gaaaacgact atagtaggtc actcccagat aacgggcacg attgaaaaag taaaatgtcc
    39541 ctatgatgat aacgattagc agcatattag aattaaaaag gcttggtgct aatactgaaa
    39601 tgatataaga taggagcgac aaagcaatgc agatatagaa actttcagag cccgctttat
    39661 tgaacagttg ttcttctctt tcgtctagta attgataata ataaaatcta tttttcatct
    39721 tcttcctccc aaaatagttg gtctagggtt ttccctaaac atctgcaaat agactggcag
    39781 agcgagagac tgggattgta ttttcccgcc tctattaaac caatagtctg gcgtgtcacc
    39841 ccgacagcct ctgccagttg accttgtgtt aaatcacgct ctacccgagc taattttaat
    39901 tttaaatttt tagccacctt cgtcctcctt atagttttaa tactcatcta ctcttaaaaa
    39961 atccaaaacc aacacaagct atcagctatt tattataacc ccattaattt gcattccagt
    40021 attatcttca gccgaaaatc agtattttag gttgtttgaa ataaacttcc tagtttactc
    40081 ttttattttt atttagtatt agctttagta agatctttta aacgaatttt tattgcttat
    40141 atttcttgtt tagtttagtt ggtttcatat cactattata taatgctttt ggaaatgagc
    40201 ctagtataag tcaaaaacgt gttgaatagt gaaagcagga gcataccaag atgactgatt
    40261 ttttactgta ttttaggtat gcaaaataat actaaatgaa actaaaatga atacttcttg
    40321 cttttcttga gagtgaaaaa gtacaataaa atgattgtag tgattaccac aagatactga
    40381 ctaatagaca tcgtatttga agtcacaaat acaggtaaag aataaaaaag gaagaagttc
    40441 aattgctttg ataaacaaat cattactagt attcatcatc atcgtattta atatattaga
    40501 gataaagagt agtaaagcta ctaaaatacc aaataccaag ccacttttca atttgtataa
    40561 atagaaaatg taagataata gaaaataaaa caggactaac aaaattgtat tgactacaat
    40621 agctactata aaatttaatc ctaaatcacc tggaataccc aatttactta ctatgactag
    40681 taacacaatg ccattcaaag cataaataat ggaaactgag atagcatata gaaaattaga
    40741 caagacgtaa atcatccgat ttttctgttg attataaaat aaactgatcg tattgtggga
    40801 aaaatccctg ctaataattc ggttagcatg tataacagcc attaacatac cgagcatagc
    40861 ataaaagttt gaaatatgct taataccaag cgatgtccct tgagtcttta gaataaagaa
    40921 tgtaagcaat atcggcggta taaacgagat aatcaaaccg agatatatta cgcctgatga
    40981 atataaatcc cttatatttt ccttaataaa attcaattta tttaatttca tgattataac
    41041 cttcctttat tttgaacgat ttagataaat atctttaagc gtctcttttt tagtttcaaa
    41101 atcaactact ttaatagagt tttcgttaaa aaatttaaaa agctcactac tttgaatatt
    41161 cccagacata gtaattctca gtccctcttc ctgaacaata tccccaaatt cttgtttggt
    41221 aatgaaaatg tctctatctg tagctgatga aaaagctatc tcaaacaaga aattatgact
    41281 atcttttcct actttttgaa atgtcaaaag cccgttctcc aagaaaagaa ccctctcaca
    41341 aatttcttca atatcttcta atttatgact cgatattaaa attcccacat tttcatgtaa
    41401 agctaaattt tttagaaccg ataaaactat ttgtgatgat tcaatatcta aaccattagt
    41461 cggttcatct aaaatcaata tatcaggttc cgtaacgaga gttagaagca aagctaattt
    41521 ttgttttgta cccaaagaat aggtctttac ttttttatta atagactgag tcaaatctaa
    41581 ctcttggatc aaacttctaa atctttcttg attgtagtca acaccatata aatttgacaa
    41641 atattttaaa ttctctaaac ctgttttaga taaaaataat tttggttctt cgattaaata
    41701 cccaacatta tcactgctta taatattacc tgaagtcggt tgattattct gaacaagaat
    41761 tttcatcaac gtactcttac caactccatt tctccctact tgaccaacaa tttcaccctt
    41821 gttaagtgca aaatttatgt ctttgagaat gattgaatga ccataatgct tacttacatt
    41881 tataacttac attttttatt cctccattaa tattattggt tttctctttg attctcatta
    41941 attttaa
//
LOCUS       DAVKJJ010000012        41339 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000012 DAVKJJ010000000
VERSION     DAVKJJ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41339
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.012"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            166..1395
                     /gene="arcA"
                     /locus_tag="VW144_000640"
     CDS             166..1395
                     /gene="arcA"
                     /locus_tag="VW144_000640"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEU9600124.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHDIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            1453..2469
                     /gene="argF"
                     /locus_tag="VW144_000641"
     CDS             1453..2469
                     /gene="argF"
                     /locus_tag="VW144_000641"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU9600125.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            2634..3581
                     /gene="arcC"
                     /locus_tag="VW144_000642"
     CDS             2634..3581
                     /gene="arcC"
                     /locus_tag="VW144_000642"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEU9600126.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIILTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            3790..5301
                     /locus_tag="VW144_000643"
     CDS             3790..5301
                     /locus_tag="VW144_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEU9600127.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYEA
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            5323..6654
                     /locus_tag="VW144_000644"
     CDS             5323..6654
                     /locus_tag="VW144_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU9600128.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNDLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVKLLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            6749..6876
                     /locus_tag="VW144_000645"
                     /pseudo
     CDS             6749..6876
                     /locus_tag="VW144_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836165.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
     gene            complement(7059..7350)
                     /locus_tag="VW144_000646"
                     /pseudo
     CDS             complement(7059..7350)
                     /locus_tag="VW144_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7338..7589)
                     /locus_tag="VW144_000647"
     CDS             complement(7338..7589)
                     /locus_tag="VW144_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9600129.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGAQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            complement(7988..8872)
                     /locus_tag="VW144_000648"
     CDS             complement(7988..8872)
                     /locus_tag="VW144_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9600130.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(9017..10168)
                     /locus_tag="VW144_000649"
     CDS             complement(9017..10168)
                     /locus_tag="VW144_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEU9600131.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGRPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPQALTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(10314..12080)
                     /locus_tag="VW144_000650"
     CDS             complement(10314..12080)
                     /locus_tag="VW144_000650"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEU9600132.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            complement(12137..15253)
                     /locus_tag="VW144_000651"
     CDS             complement(12137..15253)
                     /locus_tag="VW144_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU9600133.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVPFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNNHEVFKQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLA
                     EVEVYTYK"
     gene            complement(15263..17557)
                     /locus_tag="VW144_000652"
     CDS             complement(15263..17557)
                     /locus_tag="VW144_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU9600134.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPRTKIGSNGQIQEWLEDYEEVEPGHRHTSPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQDREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            complement(17616..18416)
                     /locus_tag="VW144_000653"
     CDS             complement(17616..18416)
                     /locus_tag="VW144_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9600135.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            complement(18413..19186)
                     /locus_tag="VW144_000654"
     CDS             complement(18413..19186)
                     /locus_tag="VW144_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9600136.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            complement(19211..19681)
                     /locus_tag="VW144_000655"
     CDS             complement(19211..19681)
                     /locus_tag="VW144_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9600137.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            complement(19672..20103)
                     /locus_tag="VW144_000656"
     CDS             complement(19672..20103)
                     /locus_tag="VW144_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9600138.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVDELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISNYDSKINLEEIVHNAQNSLFNVKQQLNVEEEEDLCL"
     gene            complement(20096..20536)
                     /locus_tag="VW144_000657"
     CDS             complement(20096..20536)
                     /locus_tag="VW144_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEU9600139.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSVSCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            complement(20548..21186)
                     /locus_tag="VW144_000658"
     CDS             complement(20548..21186)
                     /locus_tag="VW144_000658"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEU9600140.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     SQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMELMAEKFKTYGQRK"
     gene            complement(21301..22704)
                     /locus_tag="VW144_000659"
     CDS             complement(21301..22704)
                     /locus_tag="VW144_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEU9600141.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKEE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFSTESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEGTAVGNIVVQLIAMGQLKGTEEAH
                     HVIEEFLQLESYYSQKN"
     gene            22880..23653
                     /locus_tag="VW144_000660"
     CDS             22880..23653
                     /locus_tag="VW144_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9600142.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTLPDQKLLDAVELFDGEWVSDIQ"
     gene            complement(24824..26329)
                     /gene="adcA"
                     /locus_tag="VW144_000661"
     CDS             complement(24824..26329)
                     /gene="adcA"
                     /locus_tag="VW144_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEU9600143.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(26339..27145)
                     /locus_tag="VW144_000662"
     CDS             complement(26339..27145)
                     /locus_tag="VW144_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9600144.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            complement(27138..27842)
                     /locus_tag="VW144_000663"
     CDS             complement(27138..27842)
                     /locus_tag="VW144_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9600145.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            complement(27842..28282)
                     /gene="adcR"
                     /locus_tag="VW144_000664"
     CDS             complement(27842..28282)
                     /gene="adcR"
                     /locus_tag="VW144_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEU9600146.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            complement(28437..29705)
                     /gene="dltD"
                     /locus_tag="VW144_000665"
     CDS             complement(28437..29705)
                     /gene="dltD"
                     /locus_tag="VW144_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEU9600147.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKSSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            complement(29698..29937)
                     /gene="dltC"
                     /locus_tag="VW144_000666"
     CDS             complement(29698..29937)
                     /gene="dltC"
                     /locus_tag="VW144_000666"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEU9600148.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            complement(29951..31195)
                     /gene="dltB"
                     /locus_tag="VW144_000667"
     CDS             complement(29951..31195)
                     /gene="dltB"
                     /locus_tag="VW144_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEU9600149.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVIT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            complement(31192..32742)
                     /gene="dltA"
                     /locus_tag="VW144_000668"
     CDS             complement(31192..32742)
                     /gene="dltA"
                     /locus_tag="VW144_000668"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEU9600150.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            complement(32763..32894)
                     /locus_tag="VW144_000669"
     CDS             complement(32763..32894)
                     /locus_tag="VW144_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEU9600151.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            complement(33125..33829)
                     /locus_tag="VW144_000670"
     CDS             complement(33125..33829)
                     /locus_tag="VW144_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9600152.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAYLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            complement(33899..35725)
                     /gene="glpO"
                     /locus_tag="VW144_000671"
     CDS             complement(33899..35725)
                     /gene="glpO"
                     /locus_tag="VW144_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEU9600153.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKKEVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKHLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELTLSPVDFLLRRTNHMLFMRDSLD
                     SIVEPILDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            complement(35767..37275)
                     /gene="glpK"
                     /locus_tag="VW144_000672"
     CDS             complement(35767..37275)
                     /gene="glpK"
                     /locus_tag="VW144_000672"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU9600154.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            complement(37434..38861)
                     /locus_tag="VW144_000673"
     CDS             complement(37434..38861)
                     /locus_tag="VW144_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9600155.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELAIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            39026..39898
                     /gene="hslO"
                     /locus_tag="VW144_000674"
     CDS             39026..39898
                     /gene="hslO"
                     /locus_tag="VW144_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEU9600156.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKIAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGVEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            39885..40865
                     /gene="dusB"
                     /locus_tag="VW144_000675"
     CDS             39885..40865
                     /gene="dusB"
                     /locus_tag="VW144_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU9600157.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLID
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(41095..>41339)
                     /locus_tag="VW144_000676"
     CDS             complement(41095..>41339)
                     /locus_tag="VW144_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choline-binding protein"
                     /protein_id="HEU9600158.1"
                     /translation="ATGWLQNNGSWYYLNANGSMATDWVKDGDTWYYLEASGAMKASQ
                     WFKVSDKWYYVNGSGALAVNTTVDSYRVNANGEWVN"
BASE COUNT        12388 a         8950 c         7392 g        12609 t
ORIGIN      
        1 ctatcctatg ttatgtataa taacataaaa ttgtgctaaa catcataatt tatattattt
       61 tccgagaaat tatagccatt gacattacct aggaattgcg ttataacata catgtaagcg
      121 gtacccaaaa tggtgccaag tcaaaatttt taaggaggaa aatacatgtc ttctcatcca
      181 attcaggtct tctcagaaat tgggaaactg aaaaaagtta tgttgcaccg tccaggcaag
      241 gagttagaaa acttgttgcc ggactatctt gaaaggcttc tttttgatga tattcctttc
      301 ttggaagatg ctcaaaaaga acatgatgca tttgcccaag ctcttcgcga tgaaggaatt
      361 gaggttctct acctagaaca actcgctgct gaatcattga cctctccaga aatccgcgat
      421 caatttatcg aggaatactt agacgaagcc aacatccgtg atcgtcaaac caaggttgct
      481 attcgtgaat tgcttcacga catcaaggac aaccaagaat tggttgaaaa aacaatggct
      541 gggattcaaa aagttgaatt gccagaaatt cctgacgaag ctaaagatct aactgactta
      601 gttgaatcag attatccatt tgcaattgac ccgatgccaa acctctattt cactcgcgac
      661 ccatttgcaa caattggaaa cgccgtatcg cttaaccaca tgtttgcaga cactcgtaac
      721 cgtgaaacac tctacggtaa gtatatcttc aaataccacc caatctatgg cggaaaagtg
      781 gatttggtct acaaccgtga agaagatacg cgtatcgaag gtggagacga gctagttctt
      841 tctaaagacg tccttgcagt aggtatctct caacgtacag acgcagcttc tatcgaaaaa
      901 cttttggtca acatcttcaa gaaaaatgtt ggcttcaaga aagttttggc ctttgaattt
      961 gctaacaacc gtaaattcat gcacttggat actgtcttca ctatggtaga ctatgacaag
     1021 ttcactattc acccagaaat cgaaggcgac cttcacgttt actcagttac ttacgaaaac
     1081 gaaaaactta aaatcgttga agagaaaggt gacttagctg aacttcttgc tcaaaacctt
     1141 ggtgtagaaa aagttcattt gattcgttgc ggtggtggca atatcgtagc agctgcgcgt
     1201 gaacaatgga acgacggttc taacactttg accatcgcac ctggtgtggt agttgtttat
     1261 gaccgcaata ccgtgaccaa taagattttg gaagaatacg ggcttcgctt gattaagatt
     1321 cgcggaagtg aattggttcg gggccgtggt ggacctcgtt gtatgtctat gccatttgaa
     1381 cgtgaagaag tgtaatcgct gttcggcatt cgtcaataga aaatgtaaaa aatagaaaga
     1441 ggaaataata aaatgacaaa ttcagtattc caaggacgca gcttcttagc agaaaaagac
     1501 tttacccgtg cagagttaga ataccttatt ggtctttcag ctcacttgaa agatttgaaa
     1561 aaacgcaata ttcaacacca ctaccttgct ggcaagaata tcgctctcct atttgaaaaa
     1621 acatctactc gtactcgtgc agcctttaca actgcggcta tcgaccttgg tgctcaccca
     1681 gaatacctcg gagcaaatga tattcagttg ggtaaaaaag aatctactga agatactgct
     1741 aaagtattgg gacgtatgtt tgacgggatt gaattccgcg gattcagcca acgtatggtt
     1801 gaagaattgg cagaattctc aggcgttcca gtatggaacg gtctaactga cgaatggcac
     1861 ccaactcaaa tgctcgctga ctacttgact gttcaagaaa acttcggtcg cttggaaggc
     1921 ttgacattgg tatactgtgg tgatggacgt aacaacgttg ccaacagctt gctcgtaaca
     1981 ggtgctatcc ttggtgtcaa tgttcacatc ttctcaccaa aagaactctt cccagaaaaa
     2041 gaaatcgttg aattggcaga aggatttgct aaagaaagtg gcgcacatgt tctcatcact
     2101 gaagatgctg atgaagcagt taaagatgca gatgttcttt acacagacgt ttgggtatca
     2161 atgggtgaag aagacaaatt cgcagaacgt gtagctcttc ttaaacctta ccaagtcaat
     2221 atggacttag ttaaaaaagc aggcaatgaa aacttgatct tcctacactg cttgccagca
     2281 ttccacgata ctcacactgt ttatggtaaa gacgttgctg aaaaatttgg tgtagaagaa
     2341 atggaagtaa cagacgaagt cttccgcagc aagtacgctc gccacttcga tcaagcagaa
     2401 aaccgtatgc acactatcaa agctgttatg gctgctacac ttggtaacct ttatattcct
     2461 aaagtataat tttagataat aaaccgtcta ccaacagcta tgagggctgc gactaatagc
     2521 tttagtccgg tcctctttta tgtaatggta atctattatt tcttataaaa tatgtgaaaa
     2581 atcattaaat tgaaatctaa acgcattcta ttgagtgtga taaaggagaa tttatggcaa
     2641 atcgtaaaat tgtagtagct ttgggaggaa atgcgattct ttcttctgac ccatcagcaa
     2701 aggctcaaca agaagcttta gttgaaacag ctaagcatct tgtaaaattg attaaaaatg
     2761 gagatgatct gattatcact cacggtaatg gacctcaagt tgggaatctc ttgctccaac
     2821 atttggcatc agactctgaa aagaaccctg ccttcccact cgactcactt gtcgctatga
     2881 cagaaggtag catcggtttc tggttgaaaa atgctttgca aaatgctctc ttggatgaag
     2941 gcatcgaaaa aaatgttgcc tctgttgtaa cgcaagttgt cgtagataaa aatgatccag
     3001 cttttgttaa cttgagtaaa ccaatcggtc ctttctattc agaagaagaa gcaaaagcag
     3061 aagccgaaaa aagcggagcg actttcaagg aagatgctgg ccgtggctgg cgtaaggtcg
     3121 ttgcctcacc aaaacctgtt gacatcaaag aaattgaaac catccgtact cttttaaata
     3181 atggtcaagt cgtcgtagct gcaggtggtg gcggtattcc cgtcgtcaaa gaaaacaatg
     3241 gacatttgac tggtgtcgaa gcggttattg ataaagactt cgcttcccaa cgtttggcag
     3301 aattggttga tgcagatctc ttcatcattt tgacaggtgt agattatgta tttgttaact
     3361 acaacaagcc aaaccaggaa aaattggaac atgtgaatgt tgcccagctg gaagaatata
     3421 tcaaacaaga tcagtttgca ccaggtagca tgcttccaaa agtagaagca gctatcgctt
     3481 ttgtcaatgg tcgtccagaa ggaaaagcag ttattacttc ccttgaaaat ctaggcgcct
     3541 tgattgaatc tgaaagcgga acaattattg aaaaaggata agttgtttta ctaataagat
     3601 gtattctatt tctagtatct ttatatcaaa ttagaaatta ttcttgaaaa catgtacaat
     3661 atttcaaaag atactagttt tagactttaa tatggtaaaa caaatataaa tagaaagcgt
     3721 tttcttgaat gtttatttaa gaaagtagtt ggttttttac actttgttag acatcaggag
     3781 gaaaaacaaa tgagtgaaaa agctaaaaaa gggtttaaga tgccttcatc ttacaccgta
     3841 ttattgataa tcattgctat tatggcagtg ctaacttggt ttatccctgc gggggccttt
     3901 atagaaggta tttacgaggc tcagcctcaa aatccacaag ggatttggga tgtcctcatg
     3961 gcaccgattc gggctatgct aggtactcat ccagaggaag gttcgctcat taaagaaacg
     4021 agcgcagcga ttgatgtagc cttcttcatc cttatggttg gtggtttcct tggcattgtc
     4081 aacaaaactg gtgctcttga cgtagggatt gcctctatcg tgaagaagta taagggccgc
     4141 gaaaaaatgt taattttggt actgatgcct ttgtttgccc tcggtggtac aacttatggt
     4201 atgggggaag aaacaatggc cttctatcca ctccttgtgc cagttatgat ggccgttggt
     4261 tttgatagcc tgactggtgt tgcaattatt ttgctcggtt ctcaaatcgg ctgtttggca
     4321 tctactctga atccatttgc gacaggtatt gcttcagcga ctgcgggagt tggtacaggg
     4381 gacggtatcg tacttcgtct gatcttctgg gttaccttga ctgctcttag tacttggttt
     4441 gtttaccgtt atgcggataa gattcaaaaa gatccgacta agtcactggt ttatagtact
     4501 cgcaaagaag atttgaaaca ctttaacgta gaagaatctt catctgtaga atctacactt
     4561 agcagcaaac aaaaatcagt tctcttctta tttgtgttga cattcatctt gatggtattg
     4621 agcttcattc catggacaga ccttggcgtt accatttttg atgactttaa tgcttggttg
     4681 actggtcttc cagttattgg taatattgtc ggttcatcta cttctgcact aggtacttgg
     4741 tacttcccag aaggcgcaat gctctttgcc tttatgggta tcctgattgg tgttatttat
     4801 ggtcttaaag aagataagat tatctcttcc ttcatgaatg gtgctgctga cttgctcagt
     4861 gttgccttga ttgtagcgat tgctcgtggt attcaagtta tcatgaacga cggtatgatt
     4921 accgatacaa tcctcaactg gggtaaagaa ggcttgagcg gtctatcttc acaagtcttt
     4981 atcgttgtaa cttatatctt ctatctacct atgtcattct tgatcccatc ttcatctggt
     5041 cttgccagcg caactatggg tatcatggct ccacttggag aatttgtaaa tgtccgtcct
     5101 agcttgatta tcactgctta ccaatctgct tcaggtgtct tgaacttgat tgcaccaaca
     5161 tctggtattg tgatgggagc tcttgcactt ggacgtatca acattggtac ttggtggaaa
     5221 ttcatgggca aactcgtagt cgctattatt gtagtgacca tcgcccttct tctccttgga
     5281 accttccttc cattcctata aaatagtgag tgaggtgatt ccatgaaaat agatataaca
     5341 aatcaagtta aagatgaatt tcttatatca ttaaaaacct tgatttccta tccttcagta
     5401 ctcaatgaag gagaaaatgg aacacctttt ggacaagcaa tccaagatgt cctagaaaaa
     5461 actttagaga tttgtcgaga cataggtttc actacctatc ttgaccctaa aggttattac
     5521 ggatatgcag aaatcggtca gggagcagag cttctggcca ttctctgtca tttggatgtt
     5581 gttccatcag gtgatgaagc agattggcag acaccgccat ttgaagcaac tatcaaagac
     5641 ggctgggtat tcggacgtgg tgtccaagat gataaaggcc cttcgctcgc agctctctat
     5701 gcagtaaaaa gcttgctgga ccaaggtatt cagttcaaaa agcgcgtacg ctttatcttt
     5761 ggtaccgatg aggaaaccct ctggcgctgc atggcacgct acaataccat cgaagaacag
     5821 gccagcatgg gctttgcacc tgactcatct tttcctctga cctatgctga aaaagggctt
     5881 ctacaggtca aacttcatgg ccctggatcg gatcaactag agcttgaagt aggaggcgcc
     5941 tttaacgttg taccagacaa ggccaactac caaggtcccc tctatgaaca ggtttgtaac
     6001 gatctcaaag aagctggtta tgattaccaa tccactgaac aaaccgtaac ggttctcgga
     6061 gtgccaaagc atgctaagga tgctagtcaa ggtatcaatg ctgtcatccg actagctacc
     6121 attcttgctc ctctccaaga acaccctgct ctcagctttc ttgcaacaca agcaggtcaa
     6181 gacggcacag gaagacaaat ctttggtgat atagcagatg aaccttctgg tcacctatcc
     6241 tttaatgtcg caggtctcat gatcaatcat gaacgttctg aaatccgtat tgacattcgg
     6301 actcctgtct tagctgacaa ggaagaacta gtaaagttgc ttacaagatg tgcacaaaac
     6361 taccaactcc gctacgaaga gtttgactat ctagcgcctc tatacgtcgc aaaagacagt
     6421 aaactcgtta gcacactgat gcaaatctac caagaaaaga ctggcgataa cagtcctgct
     6481 atttcatccg gtggtgccac ttttgctcgc accatgccaa attgtgtagc cttcggcgcc
     6541 ttattcccag gagcgaagca gacagaacat caggcaaatg aatgtgccgt tctagaagat
     6601 ttgtaccgtg ctatggatat ttatgccgaa gccgtctatc gacttgcaac ttaatcaggc
     6661 aactgtttct accaaaaaaa atcgaccgat taatgaactg caccccaaaa gttagacaga
     6721 ataaatctaa cttttggggt gttttattat gaaattgaat tatgaagata aagttcagat
     6781 ctatgaacta agaaagcaag gacaaagctt caaacagctt tcaaaaagat ttgtgtggat
     6841 gtttctggtc taaagtcatc tgaatctttg agatgagctt tataaatcgc ttttttcagt
     6901 ttttgcactg gtgtttcgat aaactcaaac tttttagccg tggtattgcc tgattttata
     6961 gtatattgaa actagaatag tacacctcta cttctaaaac atttttagaa atcgatttga
     7021 ctgtcctgat cgatttgtcc tgttcttatt tcattttact atatttgagc cacttcgtct
     7081 ttaacggctt tattcataag ctcttgtaat ttttctttac tatcaattac ttctgatttt
     7141 ccgttgtaat ttattgtaat aggttttaac ttacctaatt tctcgacacg ctcattaatt
     7201 tgatcttttt ttgaaggctg catatgtttt tcctaagatt ttttcgaaaa tatatttatc
     7261 agatagcggt ttgccttctt cttcagcttg gtttttgtat tgatttgaaa cataaggaac
     7321 aaatccttca tagtaaccta atgctcccat aagttcaaaa gcttgttttc taattcaaac
     7381 cattgcaact cagatttcag cttttcagat aaatcctgct catccaaata atgacttgaa
     7441 attagtgctg aactcgtttc tgtatcctgt acaggctgag cacccatacc agcaaaaaat
     7501 aaactcgttc ctagcaagac cgaacaagct cctattgcat atttcctcaa agaaaaacgc
     7561 tgctttctct caaattgaaa ttctttcatc tcatctccca tcattcatta ttactgtata
     7621 ttttgtatat cagaaatagt ttgtattcac aaatctttct agttattccc ttatcattcc
     7681 taattaaggg agataacata caataatttt tagttaaatg tatatcgatg ttttttgttt
     7741 ttcttaataa acgcaataca aaaagagcct gttgccaagc tctttgtact caatgaaaat
     7801 caaatagcaa actaggaaac tagccacagg ttgctcaaaa caccgttttg aggtagtaga
     7861 taagactgac gaagtcagct caaaacactg ttttgaggtt gcagatagaa ctgacgaagt
     7921 cagtaacatc tatacggcaa ggcgacgctg acgtggtttg aagagatttt cgaagagtat
     7981 tagtctatta tttcttctca gcgcgaaggg ctgacaagat ttgtgttcgg atatcatcca
     8041 caccatttgg agtatttggt aaaaagatag tttgatttcc tttagaggca aaggtattca
     8101 aggtatccaa atactggttg gtcaagagga tagacatgat ttgttcttct gtcatgccaa
     8161 cattggcttc cttgagttcg gtgatagact ctgccaatcc atccacaatc gccttacgtt
     8221 gttgggcaat ccccacacca tgaaggcggt ctttttctgc ttctgcttca gctgcagtga
     8281 caattttaat cttgtcagct tccgccaatt cttgtgctgc gacccgctta cgttgcgccg
     8341 cattgatttc attcatggat tgcttaactt ctgcatccgg ttcgactttg gtaatcaagg
     8401 ttttcacgat aatgtagccg taagtggtca tttcttctgc tacttggtgt tgaacttcaa
     8461 gggcaatctc atcttttttc tcaaacaatt catccaaggt taattttgga acagaagagc
     8521 gaagagcatc ttcgatataa gatttaatct gagattctgg acgtatgagt ttatagtaag
     8581 catctgtcac gctctgctcg ttgacacggt actgagtcgc tacattcatc ataacgaaca
     8641 cattgtcctt ggtcttagtc tcaaccacaa tatcactttg caacaagcgc aactgaatcc
     8701 gtgctgcaat cgagtcaatc ccaaaaggca agcgaatatg aataccgcta ttagcaacct
     8761 tttggtattt cccaaagcgt tcaataatcg ccaccgactg ctgacgaacc acataaactg
     8821 tactcagtgt gactatcacc aataggagca cacaaacaat cagaaaaatc atgaaaaata
     8881 ttgccataat ggaacctcca caagtatttt tctagtatta tagcacattt aaagaaggct
     8941 gtgccgtttt tactgcgatt tttcctgaaa tgtcaataat tagaggtgaa ttgtcctatt
     9001 gtcgtctaat ctcttgctaa aataactctt tataaaaggc aatcgtttct tctaaggttg
     9061 gcataaatgg atttcctggt gcgcaggcat caatcaaggc attcttagaa aggtattcaa
     9121 agtcgaaatc tttttcttca ataccaagtt cagtcaattt cttaggaata cctactgtct
     9181 cagaaagctt ctcaatctca gcaatcgcat aatcggcaca ttcttgatct gatttacctt
     9241 ctacatgaag tcccaaggct ttggcaacat tgcggaaagc ttctggtaca cgtttagcat
     9301 tttcacgctc tataactggt agcaacatgg cacagcaaac accatgtggc aaattatata
     9361 ctgcaccgag ttggtgagcc attgagtgaa cataaccaag accagcatta ttaaagctca
     9421 taccaccaag gaagatggca ttgaccatac cttcacgcgc ttcaatatca tgtccatttt
     9481 ctacagcacg aggaagatat tccttgataa gctcaatagc accaatagac agtttcttgg
     9541 ttacatcata agcaccaggt gttaccaaag cttcaacagc gtgagtcaga gcatccatac
     9601 cagtagcagc agtcaaggct tgaggtttgg aaagcatgag ttccgggtca ttgacagaga
     9661 taagagcaag gctattcttg tcaaccatta ccatcttaac cttgcgttct tcatcagtaa
     9721 tcacatagtt aatagtgatt tctgcagaag ttcctgccgt tgtattgata gccacaactg
     9781 gcaagccttt tttagcagac ttatgaagcc cttcataatc ctgtggtctt ccaccatttg
     9841 tagccatgat agaaatacaa ctagctgcat cctgtggaga acccccacca agactgatga
     9901 taaaatcaca tccatgttct tgcaaagcag ctaccccatc tgtgacattc ttacaagtag
     9961 ggtttggctc tacatcgcta aagatgacat attcgattcc ttctgtatcc agtggtttta
    10021 aaaccttagg taaaatgtca ctgccttcga tgtacttatc tgtcaccaaa agtgcctttt
    10081 tatagccaag ttccttgata taaggaccca cttcatttac aacaccttta ccaataaggt
    10141 tgactgccgg aacataaaat gtagccatat aattttcctc ctgcgccttt gcgctatcta
    10201 tttaacatca ttatattact ttttgtattc gttttcaatt atttgtgaat tgtttaacaa
    10261 atctttttaa aaggatagga gggacgtaag ttcacctcct atataaacaa gttttattta
    10321 tgtagtggtc ccaacaactg acatgcacga tagtctgctg attctagatc ttctgttcca
    10381 aataaggacc aatttttagg tctaaagata tcttcctcag gtacattatg catattgaca
    10441 ggaattctca acatagaagc caaggtaatc aagtctgctc caatgtgtcc atatgttatg
    10501 gctccgtgat tagctcccca attattcatg acgtcataga cagacttgaa agcacctttt
    10561 cctgtcaaac gtggagcaaa ccaagtagtt ggccatcctg gatctgtacg attatctaaa
    10621 gtatggtgaa catcttcagg aagttcaagt gtataacctt ctgcaatttg tagcactgga
    10681 ccaacccctt ttagaagatt gagacgtacc attgttactg gcatatcccc cttcgtcaag
    10741 aaacgagttg agaatcctcc tccacggaag tattcgcggt ttgctggtgg gaagtctgta
    10801 ttttcaagca tagcctgcac ttcactttct tccaactccc agaatggttt cataacaggt
    10861 ttgccatctc gagtagcttg acctgtacca tccaatgtac aagaaccaga gttgattaga
    10921 tgtaagaagc cagctgcagc acgaccctct aaagtatgtc ccgttacacg tttaacagcc
    10981 tctgggctcc aataagtacg cacatcagca aagatttgtg gagtatttgt taatagataa
    11041 ttaaagagca tagacacacc atttagtgaa tcattctctg tcgcaaatac aaatggtttt
    11101 cgaataccat tccagtcaaa ctgagtattg aggaaagttt ccataaaatc tccatttgga
    11161 aaatggtctg tccactgacg ttgaccttgg aaaccagcta ctaaagcatg gtgaccaacc
    11221 gcttcttcct caaaaccaag ttcagcaagt cttgggttac caaccattaa gtcacgtcca
    11281 atcatgaaca tcttaataac aaattcccat tgtttttctt tctcttcttt agaaagaatc
    11341 aaatcctcac gatttcggtc aaccccttct ttgatatgtt cttttaccca aaccatagca
    11401 cgttcgaact cttctgggtc ataaatacca cggtccatac ggcgcgtgaa ctccgtcata
    11461 tctaccgatt catttcgcat tcctaagtat tcttggaaga aatctggatt tacaatagaa
    11521 ccgccaatcc ccatcgaaac actacccatt gataggtaag cagtgtctct catcaagcca
    11581 gttgcaagag ctgcacgcgc ataacgtaaa agtttttctt tgacatcttc tggaatatct
    11641 gtatcattag cttcctgaac atctctacca taaatcccaa aggctggaat ccctttttga
    11701 gcatgtgaag ctagtacagc tgcaagatag acagctcctg ggcgttctgt cccattaaat
    11761 ccccaaatag catgaggaat atctggagac atatccatag tttcactacc ataacaccag
    11821 catggtgtaa ctgtaattgt tgcgcaaaca tttgattttt taaacaactc atgggaagct
    11881 gcagcctctg gaacacgacc aatggtagat ggagaaatca cacattccac aggttcccca
    11941 tctggatatt tcaatgtgct tgaaatcaaa tctgccacac ttttagccat gttcattgtt
    12001 tgtacttcaa gtgattcgcg tacaccttga cgacgaccat caatagtcgg acgaatccca
    12061 atacgtggat gttgaatcat actattttcc tcttattttt ctgtttcttt catactaccg
    12121 atgataattt ttaaaattac ttataagtat aaacctctac ctctgccaaa cttaaaggaa
    12181 ttcctgcgtt tctcaaagaa attctaacct tttttccctt cagccctttt aagtctatgg
    12241 atagattatt gctttctaaa ctgtcaatat gttgtttaaa aacttcatga ttatttgaat
    12301 catatataat aatatcaaaa tttgataatc tctgaatttc agcatctgtt ctattataaa
    12361 taattaactt ttctaattct tcggtttgtg ctaaatctat ctcccaccat gaaggagaat
    12421 ctcctttggt atgagtcact gaatgatgac cataatctcc gtttttattt ccatcaactg
    12481 ctagacgaga aaaaccattg taatccgttg aactctgtcg agtttctttg gttaatgcaa
    12541 tattagaaag tttcttcgga gttctctttg aaccatagac ttcaacttcc gctaaactta
    12601 acggaatagc tgctgatttt aattctattt tgactagctt agcccctaca gaaggtactt
    12661 ttaaagataa caaaccatca actactttat caaaatgtct tctaaaaact tcttctcctg
    12721 atgaagatag aaaaataaca tcaaaattag ataaacgctg aggttcggca tctgttctat
    12781 tataaatatc aactttagaa accgtaaact cttctcccag atcgacctgc caccaagcgt
    12841 tagaatctgc cttagtatga gtcaccgaat gatgtccgta atctccatct ttattaccat
    12901 caactgctaa agcagctaca ccctcataat tggttgaact ttgagtagct accttatttg
    12961 tagagactac tttatcttct gttattttat ctatatcctc ttcagattgc ttaccatctg
    13021 attctctaaa aacctctact tctgctaaac tcaaaggcac tccactcgtt agtaatttaa
    13081 ctttaatata ccttgctcct ttttctttga aatctagact aacagattca cccgacaaat
    13141 tattaacatg tttcttagca acttcgtttc tattattgtc atatagaatc acatcaaaat
    13201 tagatagacg ttgagtctca gcatctgtgc gattataaat tttaacaagc ccaactttat
    13261 ccattttttt caaatcgact tcccaccaag agggattatc tgccctagtg tgtgttaccg
    13321 aaccagagtt aaaattacca tttctgttac catccacagc tctattaggg tctccattgt
    13381 aatctacaga actttgtgtt gttggttttg catatgctat attcaaatta ccatcattaa
    13441 atttatctgg tgttgggaaa gggactgggc ctgtcccttc agtatttata gacatctcta
    13501 ccattccatt atgattaacc gtaatggtat aaacatgggt attctcatcc caattttctg
    13561 tataagtata atgattttta tcgccactta tatttatctg aggtttatga ccagtatgcc
    13621 cttttaatgt aagtgttgtt gtcctaaagt cattatctac aggattgatg gaataatttt
    13681 tgcttatcca gttcgctaac tctaattctt ctttcttcca actttttgat gcatcaaaat
    13741 ttcctgataa tgcccacata gctgtcttat ctgtcctgta attgttcaat aaaatatgta
    13801 aattatttgg attttcttta acaacagcgt aagtatgtgg cgtcaaatct aacgataacg
    13861 actttgtatt attagtatac ataggcaaca ttacttgctg atttttattg atattagcat
    13921 tactattata aatataccag gaattaccta cacgctgagc atacccatct ccttcataaa
    13981 gttttggata aagcgagttt aaatagttga ctttactaga caattcctca ctatttttag
    14041 tcaaaatttt tgcattaggg aatatagatg aaatcttttc cttatcaatt ttctcatgta
    14101 ttacaggaag aatatgatat ctcccattat tatataaagg cattgtttca tcattcgaat
    14161 aaagtccttg ataaaatccg tttaatgaac taatcctacc ttctccattc caaaatacag
    14221 cttttgttct atttacaact tcttccttac ttggagctgg attttgtata gcatgtctaa
    14281 agaaaggaat aataccttta gtaaatgctg gagttggtac atcatttgtc ataaatgtat
    14341 acgcggcaca ttcaaaatta taaactgtgc ctcccccagt atatacattc atcatttcca
    14401 tagcaatcat tgattctggt tccgatgcat aggatctcat atccctagct cttccagttt
    14461 caaatgtgtt tgtataatgt ttttcccacc atttccatgt atctgttgat gagccccagt
    14521 tatcacataa gccactcaac caaaatccac taacgataga atctgtaccc gcatcatctc
    14581 ttattggcgt atttttagtt gccaacacca aatttttatg atatttttga ctcgcttcaa
    14641 agaatgtcgg atcattcata atagtttccc agaaccattt ttcatgatca tgccagataa
    14701 aatgcgctcc atatttggca caaacttcca aatatttagc actatgcgga gctaactggt
    14761 tattgtaaat ccaataattc tcaatattta aaacaccttt taacacacta tacttttgga
    14821 attgttcatc taaccactct ggaggaactg tattacgctc tccagccgac ataataacca
    14881 agaaaactgg aatgtttaga ctttgagctt cctccaacat cttgacatac cattctctca
    14941 attctttcgt atctcgtgga atacaacttg ttggtttaca gacttttgca ggatgaagtt
    15001 caatcgctgc atatggcttt acatcttcag gaatagcttc ccatgctccc ttcaatgtgt
    15061 tcccccacca taatccgtta ccattatcat tgccatacaa cggagaaagc aacaatggcg
    15121 attcattatt aatcgtcgtt ctcatttgaa cacgattatc agccttaact cctgctaaca
    15181 atccaaaaca taacatccca atacatacaa acagtattgt gattaatttt ctttttattt
    15241 tttctttatt catgacttat acctaaaaat ttaactcaat aattttttct gaattaaata
    15301 ccaattcaat attttgtaca tcagtatttt tgccatatat tcttactctt actttttgat
    15361 ctttgtttcc tccttccaat tttaggaatg ttatatcccc atttttccaa gcaaacgata
    15421 ccttatatcc tcctcttact ctgaaacctt tcacttctcc ttctgaccaa gcagaaggta
    15481 aagctggaat tagtgataac caattatgat ggctctgtac taataattca caaattccac
    15541 tcaccaaacc taaattacca tcaatttgaa atggtggatg gtcaagaaat aaattgccaa
    15601 gagtcgcatt atttaacaaa ccattaatct ggttataagc aggttcacct tgatatagtc
    15661 tcgcaaaaaa atgaatcagc catgcagcac tccaacctgt ttgtgtacta gcatgcaaac
    15721 cacttactaa ccaattatta atcgcttgct ccctatcctg tgaagataaa aagttagcgt
    15781 ttgataatct cctattgata gtgattttag ctgcttctgc taattccgga gttttatgaa
    15841 tatcaatctc attataagga taaagcccaa atagaggtga agtgtgtcta tgaccaggct
    15901 ctacttcttc ataatcctct agccattctt gtatttgccc attactacct atttttgttc
    15961 taggtaattt cttttttaac tccttcacac gactaataaa atccgaattg tctcctaatt
    16021 gttttgcaat tccaatgcat gaatcacaaa aatatcttaa aatttgatta tcaattgtag
    16081 atgatagaca agcatttcct tcaataccat tttttaagcg atatttattt tccggtgaga
    16141 cacttggacc tgtcatcaag tagccatcca cctcaaataa ataatcttca aagaaaagaa
    16201 atgcttcttt tatcatttca aaatgttccg taagaatacg ctcatcttgg aaatataaat
    16261 agtgttccca aatatgagta cataaccatg gaatagttaa tacccaaatt gcagccccca
    16321 tggcatgaga ttggggagcc gtatcgccaa aaccatccgt attatgatgt gctgtaaaac
    16381 ctctagctcc atacattttc ttagcggtta gtcttcccgg ttctctcatt ctttcgagca
    16441 tatcaaataa tggatattct acttctggta aatcacatgg acctaccatc caataattca
    16501 tttgagtatt aatattaatc gtatatttag aaccccaaat tggatttaat tcatcacacc
    16561 atattccttg aagattggca ggtaaaccat tcggttgact agacgatatt aacagatatc
    16621 ttccataatg aaataacaag ttagtcaagt agttactata ctttttagtg ttttcaagaa
    16681 gtagattcgt tggaatgcta agacaatctt tactatagtc tagtttaaaa tcaactctat
    16741 taaattgctc ctgatatttt tttacatgtt catctttttc tgtaaagtaa tcaatactac
    16801 taaattctcc ctgaagagaa gaaatatcta tatttcccca ataatccgtc attgatttga
    16861 gataaagaaa tacctctgta gcattccgaa taactattgt ctctcccaat acacttactt
    16921 caccatccgt aaccttagaa tgacatacta ctttaaactg aacacctttt ctacctccag
    16981 cagaggccga cattaaaatt gtacttgaat ccagtttaga tacttcatca ttaaaccgtt
    17041 tatttctacc caaattaatg tttaaattta atgtgttttg aactgatgac actatacggc
    17101 aacataaaat attcttatta aaactcgtaa aatattctct ttttatttgt aaattacaac
    17161 tattaggctc aaacacaaca ttagaaatag ctgtatctaa atctagctct ctttcataca
    17221 atgaaagagc acaagactga atatctatat gctcaatgta aagttcccca agtaattcat
    17281 agtggctttg atctcttggg gtagcaaaca ttgttaactt tatcaattct tcggctttct
    17341 gaatttctcc atctaaaaga tattcccgaa tttttttaag atgcaatagt gagtctggat
    17401 tatttctatc tgactttcct ctataccaaa tagtctcatc gtttagttga atacattctt
    17461 ttgtagctga accataaatc ataccaccta aatgaccgtt cccaatcggc aaggcttcat
    17521 tccaatttga ggcagctttc ttataccata atttcactct ttacctctct tccaaaatgg
    17581 aggtgatatc ccaaatacca cctccacact ttctactact tcaaaattcc taaaacaact
    17641 aggataactc caatagccat aacactaaag attagtacaa ccggattctt tccttttcta
    17701 attaatttac acattaataa agtcaaagcc atagggataa atcctggaat taattttgta
    17761 atcatttctt gaacagaaat aacaagttcc ccctgcttaa attctaatcc aaaattaata
    17821 ccaaccattg atggaatcaa accacccact agtactagcc ctaatgctgt cgccatactc
    17881 gtaacgcgat tcaatgttcc ttttgtatta ttttcttgga taagactaga acccttagta
    17941 tacccatatt tcaaaccgaa atatttaaca ggaatattaa taatattaaa caatattaag
    18001 gcgataaaga tacctaaagc accgccgtct ttagaataag acgcaccaat actaaaacag
    18061 ataggaacta gtgtcagcca gaacaaacta tctcctagac cagccagtgg ccccatcaag
    18121 ccaactttaa taccagtaat tgaagctgca ccttcatttc cttcttgttc ttccatagcg
    18181 gaagtaactc caagaataaa tggtgccgct gtttgatgag tattgaaaaa ctcaaggtga
    18241 cgtttcattg caggagaagc tgaatctttg tctgggtata gttttttcaa tactggaaga
    18301 attgtatata gaaaacctag gttctgcatt ctctcatagt tccatgaaga accatacata
    18361 aaacttcgtt tgaaaacttt tttaagttca actttagtta ctttattatt catcatagat
    18421 caagatcctc ctcaacatca ctacttgaag ttgtttcttg tggtttactt ccaatcatat
    18481 cgtagaagta tgctaccgca ataccaacta gtgagatccc aatggttgac attcctccat
    18541 acacagaaca aatgaatcca atcaacaaga atacccacat atttttcttg agcatcatat
    18601 ttaaaagcat accaaaacca attgaaggta gtaaagcacc tgcagagtta agtccctgca
    18661 taacgattgg tggaacctta gcaaacaagt ctgccacata ttcccctcca agcataatag
    18721 ctaggaaaat tggtacaatt ttaaataacg tgattaaaac taaacttgaa taatgataaa
    18781 aaccgatctt tgaataatct ccgtttttag catcattatc agcttttttc acaaaataaa
    18841 catctaaagt ttttgcaaga acatccaact gttgggtagc aactgcaatt ggaactgcta
    18901 tagcgatacc agcagtttct ccttgaccag ataaaatacc aaatgcagta ccaataatcg
    18961 cacctgaaat agtatctggt ggagtgtaac ctccaatacc tgttacaccg agccaagtta
    19021 attcaagagc tgaaccaata agaacacctt gggtgaaatc tcctaagact aagccaacca
    19081 ttgcacttgt aactaacgga cggtgaatca taaataacgg tccattatag tcaatagtgg
    19141 caataatccc aactaatgtc accagtaatg cttgtgtgaa tagcatatac tgtacctcct
    19201 taaattattt ttataaaaat ttttccaaca ttgtaacttt atcattagga accatttgaa
    19261 tttcaactct cgttccctta tcaactattt tcttaaaata atatttgtct tcttcaccta
    19321 gagctacacc tcccgttacc ttttcatttt cctccgtttt actcatctgt cctacattga
    19381 ggtactccaa ttttaaattt ccttcaatag aatcatacac atcttttgga tttgtatata
    19441 tcagcattgt tctctttttt attggcacag agtttaaaac ttccacaaac cgttttacac
    19501 taaagaaaac aatttttaaa cctactggtg cagaaatctt taaaatagat tgtcgtgttt
    19561 tatcttctga gatgcgatca ttaacaatga taacttgctc aatatcatac ttttttagcc
    19621 acgtagtgac aacttgacca tgtaccagac ggtcatcaat acgaacaaac tctatagaca
    19681 taaatcttct tcctcctcta cgttaagttg ttgtttaaca ttaaacaaac tattttgagc
    19741 attgtgaaca atttcttcta aattgatttt tgaatcataa tttgatatta attccactag
    19801 gagagggata ttaaaccctg ttacaatatc aactgaatcc aaatttaaaa accgtgacaa
    19861 agcaacatta ttaggacttc ctccaatcaa gtcagtcaaa acgataacct cttgatttga
    19921 gtctaacagt tcatccactt gatttttaaa ataatgctca aactctacaa tattctctcc
    19981 aggcatcaaa cctaatgtcc taactctatc atttgttgtc tcaccaacaa acatttctgc
    20041 ggtcatgaga gctccgctag caaaattacc atgactggca actaaaattc ctctattaaa
    20101 cattttctct ttcaacaact actcctttct taaggataat attagcataa agtgaagttt
    20161 ctcctgtagc aacaatagca taagctttct tactacgttc ataaaagtct tctcttctaa
    20221 gataagtaat cgttttaaga tctgtaccat gaccttcaat catctgtcta taggtacccc
    20281 atatcttagg aatatcatca cccgaaacaa cgttcataaa ctgaattgaa ctatcgacgt
    20341 aactatctaa tggcattaaa tacagaatgg aatctaataa ttctggaata tttacaccat
    20401 cacaacgaat taatttattc gcacatgaga cggaagggta attcgcgtca gctaatacta
    20461 tttcatctcc atgtcccatt tccattaaag tcttcaataa atctggagaa atatttttcg
    20521 gtatatgttt taacattaat atcctcccta ttttctctga ccgtatgttt taaatttttc
    20581 tgccatcaat tccatctcct catcaggaag aactactggc tctccaaaat tcttagctaa
    20641 acaataaatt tttgcacaat attcaacttc ttcaacaata ttaaatgcat tcaataaatt
    20701 ttgtgaacct gctaaaattc catgattcgc tagtaaaact gctctacgac cttccattgc
    20761 tttagctgca ttcacagcca attctttcgt gccatatgtt gcatactcag ctacccgaac
    20821 atctttccct gccactgcaa tcatataatg actcgctgga agtggttctc tgagacaagc
    20881 taatactgtt gcataagttg tatgagcatg gataattgca tcgatatcat cacgagtttg
    20941 atattgaatc aaatgcatat accattcgct agatggcaag cgttctccct ctacaacatt
    21001 tccattaata tccatcacta caatatcgga ttctttgatt tcaaagaaat caatacccga
    21061 cggggtaatt gccatcaatt gtttttcacg atcgaaaaca ctgagattcc cacctgttcc
    21121 tttcgtcaaa tctgtttcta ctagcttctt gccatactta attaattctt gttttacatc
    21181 tgacattctt gtcctcctca tatcaaaaca ctaaatattt ttcaattcac caattatccc
    21241 cccttcttgt acaaaactta agaaggggga gggaggcaaa tttacaaact ctcaatcttt
    21301 ttaattcttt tgggagtaat aactctctaa ttgtaggaac tcctcaataa catggtgagc
    21361 ttcttccgtc ccttttaatt gtcccatagc tatgagctgc acaacaatat tccccacagc
    21421 tgtaccctca gtcgaacctg taagaacctc tttaccagtt ctatcagcaa tcatttggtt
    21481 aaaataactg gctctagcac ctcctccaat cacatatatc ctcttataaa ccttatgagt
    21541 tagttcttct agtaactcta tcgctttcct atacgtttca gctaggcttt cataaacaat
    21601 cttaaatagt tgtccatctg tccactctct agtttcatga tgataagcta gatattctgt
    21661 caaagtcttg tgcatatcag attctgttga aaattcagtt gattcagtat caatcagagg
    21721 aagattttct ttttctttct ccaccattgt cctaatatca tcaaaagaat aggctttccc
    21781 tcttcgttca aatgaacgtc ttagttcctc tatgatccac aaccctgtac aattcttcag
    21841 aaatgtaatc actccatctt taccgacttc atttgtaaat ccataactga aggattcggt
    21901 agtaagaatc ggtgaagtaa gttccactcc aaccaaagac caagtacctg atgaaataaa
    21961 taaactacct tctgtcttag gtactgagac aatcgcgctt gctgtatcat gactacaaac
    22021 attcacaaca ggaatatcgc ctaaaccata ctcctctttt atccttccaa gaacatttcc
    22081 ctctgaaaca atttcaggaa gtaaagatga atccaattca aatagtttta agatattctg
    22141 attccaattt tgactcctag gatcaaataa ttgagttgtt gaagcaatgc ttttttctgt
    22201 agcaaactta cctgtcaaga gataattaaa caaatctggc attaaaagaa tcttattggt
    22261 cttatagaaa gagtcaggag attcttgacg tgccttaaag agttgaaaca aggtatttat
    22321 ctccataatc tgatttcctg tctctgaata cagtttttct aattcagtca tttcagatat
    22381 ttcctttaac actccctttg ttctttcatc acgataatga acaggttgtg ataacagctt
    22441 accttcatta tcaatcagtc caaaatcaac tccccatgtg tcaataccga tagataaaat
    22501 cttgtaacta gtattagcca agcggatact ttcaagaatt ttagctagta gaaagtcaat
    22561 atcccaagat aaatgccctt ttactctaat aggtagatta gaaaagcgat ttatttcttc
    22621 cattactagt ttattttcag aaaggtaacc aacgattgct cttccagaag tcgcacctaa
    22681 atctattgct aaaaccgctt ccatttttct tctctcttcg tccttgatta actttattat
    22741 aatccccacg ttttattttt tcaatatgtg aacgcttact tgaataatgt tcaaatgtta
    22801 acccaatatt cataaaaata attcacattc ctccaatttg tgctataata tagttgaaat
    22861 gataaattag gaggagctta tgataaaaga tgaacgtgta cttgaattga ttgaaattat
    22921 caaaaagaaa aaaagaattg ccgtaaaaga gctggcagaa atcactttct ccagcacaag
    22981 taccttacgt cgtgatttaa ttttcttaga aaatcaaggt cttatcaaaa gaaagcacgg
    23041 atacgtgacc ctgtcctcta tgaacacaat tgaactttct catcaaatac gtgaaggaga
    23101 aagtactagg caaaaaagac taatcgctag tctcgctaaa gactttattc ggtctggtat
    23161 gtgtatctat ctagattcta gtactactgt ctacgaactt tgtccctatc tttctgaact
    23221 tgataatttg attattttta caaatggttt acatactgca caaaccctat ctgaaactgt
    23281 taaagatagc tccaaaatct ttatcacatc tggcgaggtc aaacatcaat cctgttccgt
    23341 ggtcaactat gataaggaaa attctttatt agatcatttt aatatcgatt tagcattttg
    23401 ttcagcaaga ggtattgatg accaatatgt ttatgaagct tctctcagcc aagctatttc
    23461 aaaaaagaat attattgaca aagcccatga aaccatctta ctgattgata gttctaaatt
    23521 ttacaagact ggatttttta aaattaatcc cctatccaaa tacacaacct ttatttctga
    23581 caccttgcca gaccaaaaat tattagacgc agtcgaatta tttgatggag aatgggtttc
    23641 tgatattcaa tgaaaatcaa ataggaagct agtctcagct tgctcaaagt acagatttga
    23701 aaattgatga agtcagctcg aaacactgtt ttgaggttgt agatagaact gatgaagtca
    23761 gtcacatata tacggaaaga cgaagctgac atgatgtgaa aagattttcg aagagtattc
    23821 gttctgtctg ctgactgatt tacaaatatt caccacatga cattattttc ttagcaaccc
    23881 taaaaacaga gactggacaa aaactcaatt tcaggagaaa atggagtaaa ccttcccaca
    23941 ataaaacgca tagtatcaag tttttgcaca cctgatatta tgcgtttttc tgattttaaa
    24001 gactttgtcg cctagaccct atatttcaag aattaagcga ccttgatgat ctaaatgtat
    24061 tgtggtatac tatttttgtc atcgattacc gattacgttc ctatgggttc gtgagaggag
    24121 gtgaaactaa tgagccttat catagagctt gctcttacta ttatagcgga tgttatagct
    24181 ggaattatct tgtatttcgt ctgcagatgg ctagatagta aggagtagac cgactgacta
    24241 gcctataaac acccgttaaa tcgctaagat acgtcaaaaa agcccttaac tacggccata
    24301 gttaggggct ttggtgttct aatgaacctt atcaattaac tacattttag catataatct
    24361 acaatagttc aagcattaag tgaggttgat gatctaaatt tagtgtggta taatactttt
    24421 gtcatcggtt accgattacg ttcctatggg ttcgtgagag gaggtgaaac taatgagcct
    24481 tataccagag cttgctctta ctattatagc agatgttata gctggaatta tcttgtattt
    24541 cgtctgcaaa tggctagatg gtaagaagta gaccgactga ctagcctata aacacccgtt
    24601 aaatcgctaa gatacgtcaa aaaagccctt aactatggca ctagttaggg gctttggtgt
    24661 tctaatgaac cttatacact aactacattc tagcatataa gcccagatat ttcaagagtt
    24721 ttatttattt tttcaggttc ccttagttct gaaaggtcta taatgaagtt agccatctag
    24781 tatcaaaaaa ccgactagct cttatgaact agtcgatttc tcatcaatgc gccaacattt
    24841 cttgggcgat ttcttggcca gataggttat ctgggtagta ggttggccag ttgtccattt
    24901 cttcaaagag ggcttcttgg cttgtgcctc caaagaagat atggaaatgt tctgccttaa
    24961 ctggggcgat attgtggtca ctaaactgaa catacttgaa ttgtccagcg tcagcatctg
    25021 tggcttcaaa taggaaacgc acgccacgat tgcctttctt gtaagtcaaa attttcttac
    25081 cgacatactt gtaagtgtat ttcttgcttt gtccaccttg aacaaattcc atagtattat
    25141 cagtaatgtt aatcttagtc acatctgtct gatagccttt tgtatagtaa gccttgtact
    25201 cagcctgggt catcttacca gttaacttag ccttgtagtc aaagacttgg tcaaacgtgc
    25261 cgtcttcaag gaaaggataa actgattgcc agttacctgc atagtcactc aaggtacggt
    25321 ccttgacagc tgcatcctcg aagtaaccat tttggactgt cttggtatcc tctgcctttt
    25381 caggttcgat tgctgggcct tcttggtctg ttgtttgttt caaagccttg aggtttttct
    25441 ccatcacgga aatgtagttt tctccagcct tggtgtcctc ttctgtcaga ctttctaaag
    25501 gattgaggac atcagttttg acacctgctt cttttgaaag tgtgttagca agggcttgtg
    25561 aggcattttc ttcaaaatag atataggcga ttttattttt cttgacatac tctgtcaatt
    25621 ctgccaagcg agcagctgat ggctctgcat ctggagaaag tcctgagatt gcgacttgtt
    25681 tgagtccata gtccaaggca agatagttaa aggctgcgtg ttgagtcaca aagctctttt
    25741 gttttgcttg agacaagcct tctgcgtaag ccttatccaa ggcttgcaat ttttcgatat
    25801 aggcagctgc attcttctca aaggtctctt ttttatcagg ataatctgct gacaagctgt
    25861 cgcggatgtg ctctactagt ttaatggcac gaactggtga taaccaaaca tgggggtcaa
    25921 actcatggtg atgaccttct tctccatggt catgatctcc ctcttcttcc tcgccacctg
    25981 gcaagagcaa catatcgcct gtcgccttga tggttttcac ttttttctta tccaaggtat
    26041 ctagcaattt aggtacccat gtttccatgt tttcattttc ataaacaaag gtatctgcat
    26101 cttggatttt ggcaactgcc ttggcagatg gttcgtattc atgaggttct gtcccagcac
    26161 cgattaggag ttctacatta gccgtatctc ctgcgacttg cttggtaaat tcatagacag
    26221 ggtaaaaggt tgtcacgata ttgagtttac catctgcctg tttttgattg gaacaagcca
    26281 ctaaaaacaa ggcacataga ctggctagta ataagctaat ttttttcacg ttcgtctcct
    26341 atttgataaa acgtcttact aaactgatta gtataaagac agttacaaaa ataatggtaa
    26401 tacttgcact tgcaggtgtt tctgcatagt aggaaatgta aagtcctgct accattccca
    26461 aaaagccaat cgcactggca agcagcataa ccgatttaaa gtttttcccc agacgcaggg
    26521 caatactagc tggcaagacc ataatggtcg ataccagaag agctcctgct gcaggaatca
    26581 taagggcaat agccacccct gtcaccatgt taaaaagaat ggacatggta cgaactggca
    26641 agccatccac aaaggccgta tcttcgtcaa aagttaagat atacatagga cgaagaaaga
    26701 gaaaggtcaa aatcaaaaca accgccgcaa tgacaaagag ggaaatgacc tgttcttcac
    26761 tgatagtcac gatcgaacca aagagatatt ggtccaaact cattgaactc gagcttttac
    26821 ccttgctcat gacaatcaga gaaacagcca gacctgttga catgaggata gctgtcccga
    26881 tttccataaa gctcttgtaa accgtacgga gatactccag aaagaccgcc gcaatcaaga
    26941 caatggcaat agtagaaaca gttggagaaa tccccaaaac cagaccaaag gctacacctg
    27001 aaagtgagac gtggctaagg gtatcactca tcaaactctg acgacgcaag atgaggaagg
    27061 ttcccaatac cggtgagaaa agactcatag caataaccgc caaaaaggcg cgctgtataa
    27121 agtcgtaaga taataaacta agcatggccc acctcctggc cattctcatg aacgttgaaa
    27181 caacgccatg gcgagtcttg gttacggact agatgaatat tgcgatccgc ataatcctta
    27241 acttcttcag ggtcatgggt aatcatcaaa acagccttgc catgatgatg ggcgctgtgg
    27301 tgcatgagtt cgtaaaattc atttttactt cctgcatcca tccccgttgt tggctcgtct
    27361 aggataaaca catcagggtc agaagcaaac atacgcgcaa tcaccgctcg ctgcttttgt
    27421 cccccagata gagaccccaa gcgtttgtct cgatgttccc acatgccaac tgagtccaga
    27481 ctggccttga tatgctcctc atcatgagca ttcaaacgac ggaaccagcc ttttctcgga
    27541 tagcgacccg acttgacaaa ttcatagacc gtacttggaa aaccagcatt aaaactggca
    27601 atttgttgag gaagataggc tattctcaat ttcttacctt gcgtatttgt ctttgaaata
    27661 gccacctttc caatgcgtgg ttgcagaatt ccaagactag ccttgatgag cgtcgtctta
    27721 gccgctccat tttccccagt caaggtaaca aattccccac tatcaacact ataattgata
    27781 tgttcaagaa caggctcctt atcataatag aaggacaaat cctctaccgt aatatatctc
    27841 attatttgat ttctcctact aaagcagtca aaaaccgctg aatcactttt tgttcatttg
    27901 gagtaaactg agtcgccact tgttcatagg ttaaaagtgt atgctcatgg tgatggtggt
    27961 gctcctcagc gattggacga gccaagtcag tcaactgata aaaaatcaca cgcgcatctt
    28021 tagaatcttt agatgtttcc aacatccctt ccttgaccaa agacttaatg gccttggtaa
    28081 ctgccgcctg actgacattg agacgacggg ccaattctga atttgttaaa gattcctctg
    28141 acaagagcat aaggatatgc tcctgagtat tggtcagggc cacctcgcta gtgcaatgac
    28201 ctattaggat ttcatgctga ttttccgcct gcaaaatcac ctcattcaaa aaagcattga
    28261 tatcctttgc tagctgtctc atatctgact cctttccttt tagacttctc ttttttaaga
    28321 gaaaaatact attctttgac attttgttta ccagttaatt atatcacaag caaaaaaaga
    28381 gtcaagaaaa aacgtgaaaa ctagtttcat tcttgaactc ttctatatta tattatctat
    28441 tgaaattctt tgacatctcc atcataagtc gcccaatctt tgctgaaaaa gcgctcattc
    28501 agatggtaag tcggagctgg tgtgggattg gataggaaag gatcaactgc cttgtcaaaa
    28561 gccaaccaac ccaaccagcc aaggtgaatg gtgtccttca taaagaaagg ctccccgccg
    28621 tccttagaaa aatctgctat attggtaaaa ccttgacttt ctaactggta gcgaatcttc
    28681 tgcaccgttt gttggtacat atcctctcgt agaccagcat agtccatcca ttttttatta
    28741 acaggtggaa tgataaaaat cgggtttacc ttagatttag aaaactgtgt taaaaccaac
    28801 tgcaagtcat tatactctgg cgacttgaga taggtaaagc ttttctgaga atcctttaat
    28861 ttcttcaaat ccttcttgat ctgctcatta tagaaataat tttccattcc catctcatta
    28921 ttggaagtat ttttttcagc atctgctttg acaacatctt ctattgcttg ataagaaaac
    28981 tggtctggca agatttttaa atacttagct acatgcttat cgtagttaac atagcctcta
    29041 accgaaaact gaccaaaaaa ggaggcttgg cgttcattaa aacgagccaa taattcaatc
    29101 atttcattgt ctgctgtcga caattcttct ttacttgcca acttctgaac caggtccttc
    29161 atagctacgt ttgggaactg ttgcagtaag cgagtcgctg catattgact agcctgatcc
    29221 ccagattgat gtttcagaaa actagtcaac tggtctccat taaaatactg ctggaaggct
    29281 gctggatcat agccattttt actgaaccac tgaggtgaga taacatacac aacttgttta
    29341 ttctccagct gtggtaacat ctgttgcatt ccaaaatatt ggttaagcga tgcagctccc
    29401 ccctgtccta aaagataagg acggtaggaa cgattgtatt tctcagctaa taccgcagga
    29461 tgagcaccgt caaaacgaag ccattcacta gagccaaaga agggaacaaa acgcacattt
    29521 ggatcagata gtgctctgac tttttgactt cgctccttaa aactatcgat agtagtagcc
    29581 actgctgaac tcttttcagc tcctagatta tgatgcatct cagtaggata aaagaaaatg
    29641 agcagaaaaa ccaacaaacc agcgatcaag accggtccga agatcatcca taagcgttta
    29701 agcattttgt agctccacaa taccagctat gattttatta gctgtattcc agtcgtcacg
    29761 accaaactct gttacaggga cacgaatgtc aaaacggttc tcaatctcca caatcaactc
    29821 aaccgttccc atactatcca agacacctgc atcaaaaaga tcttcatcca tcatgtcaga
    29881 aacatcttcc ataaacaact catcaataat ttcaataact tctgatttga tatccatatt
    29941 ttatttcctt ttatttttta aaccatagat tattcaagaa tccagaaaag attaagaatg
    30001 acaacatgac aacatggaaa gtgataacca tgccaagcaa ctgaatccag cgattctcag
    30061 gtagggcagc cttccctgct tttttccgtt ccttattgag cgtttttttc ttgcgaaccc
    30121 aggcatcatt gatgaccaag cctagtccat gaaagagtcc ataggcgata tagtaccagg
    30181 tcacaccatg ccaaaatccc ataatcagca tatttacaat gtaggccatg cttgaggtta
    30241 cattacgatt tttaaagact ttctttctgg ttaacaccat caccattcgc ataaagacaa
    30301 agtcacggaa ccagaaggac agactcatat gccagcgatt ccaaaactcc tttaaatccc
    30361 ttgataaaaa gggcttgtta aagttgatag ggctacggat tcccatcaag tttgagatgg
    30421 ccaaagcaaa catagaataa cctgcaaagt caaagaagag ctccagacca aaagtataca
    30481 taactgccaa ggcatagaga ttaaagaagc cacctgactg caaggctaaa ttcttcagag
    30541 gaggtagtaa ggtctctcct aaaacatgag ctaggataaa cttatacaaa aagccccgca
    30601 tgatatagcg gacagattca tccagcatat ccatcaactc atctcgctca ggaatggcct
    30661 gataattttc attaaatcgc ttaaaacgat cgattggacc actcgagaaa gttggcatga
    30721 agagaaggaa acggaggaat tcccagaggg taaaatcctt aatcactcca tctctcagct
    30781 cgatgataat cccaaccgaa cgaaatgtca ggtaagaaat tcccaagaac ccaagcaaag
    30841 actgcgttcc attgatagct ggttgcacct tgacaaagat aatcggaagt agggacagaa
    30901 aactaactaa gtagaagacc cacttgccat ccttgctttt tcgataatgc ttgtagaaaa
    30961 gcaggagcaa tatttcccag caaaggtaaa tacccaaggc agctagttga ttggtctttc
    31021 cacccaccaa catggtgaca ataaagaaga gacttaccaa cacttcatac caggcaaagc
    31081 gtttcttgaa aaagagacct ataaagatgg gcaaggttgc agcaatcaca taaacaaaat
    31141 actgaggatt gccatatggc tctaaatgag gaagctgttg aaaaaactcc atcatctctt
    31201 attcacctcg ttaatcaatc ctttgatgtc aatctttcca tttggagtta gtggcaaact
    31261 gtctcggtaa aggaatttag atggcatcat gtaggacatc atgatgtctg tcaggtcttc
    31321 cttgatggcc ttggtaatat cgatatctcg ctcaaactgc tcacgaacac cgtcttttaa
    31381 gatgacataa gccaatagat tttgtacctt gtggtccttg ttatagcgcg gtactgcgac
    31441 agcagattcg ataaagcgag acttgttgag gttttgagag acatcttcta actcaatgcg
    31501 gtaaccgtta aacttaatct ggaagtccat gcgtccgccg tagagaagca agccctcatc
    31561 tgtcatggtt cccacatcgc ctgtgtgata ggctggcaga ccttcaaact caaagaaggc
    31621 ttctgctgtt ttttcaggat tgttcatata accttttgaa acagctggcc cagaaacaat
    31681 gatttctccc tgttcgccat ttggcagttt atttccttcc tcgtcaatga taaaggttgg
    31741 agaatcagcc ttggtatagc cgattggtag gcgtttgaga gtcgctaaca tctcgtctgt
    31801 aacagcaact gctgacagag ctactgtcgc ttctgttggg ccgtaagcat tgatgatacg
    31861 ggcatttggg aaacgctcgc gcagtttttg agctgttttg accgtcaatt cttcaccatc
    31921 aaagtagaaa tgcgtgattc caggcatttt ctcactgttg aagtattcag acaacatggc
    31981 catatctgca aaggatggtg ttgatgtcca gatagcgatt ggcaatgaaa agatagccgc
    32041 aaagagttgc ttaaagtcct gagtgatgac tgaaggaaga gtgaaaagcg taccaccaag
    32101 tgccaaggtc ggtgcccaat acatgacaga caagtcaaaa gaataaggtg gctgtgccag
    32161 catttgcgga cgactcggtg tcgcaaattc cttatccgta atcatccagt ttgtaaagct
    32221 gaggagatta tcatgtgaaa tctgcactcc cttaggctta ccagtcgtac cagaagtaaa
    32281 gataatgtag taattatcat ctcccttgac tggatgcgtg atttcatagt tgttcccttg
    32341 ggcaaaggct tcttgaacct gagctagatt tatcattggt gtagaaacct gctccaaggg
    32401 aaaggctgaa atggcaataa tcaagcttgg ctctgctact tctaaaatag ctgaaactcg
    32461 ctccaaggcc gaatggctat caattggaat gtaggcatga cctgacttag tcagcgctac
    32521 aaaggttgcc aacatttcat attcttggcc accaaaaaca accacaggag acttctcagg
    32581 caagcctagt tggtcaatga ctgcagctaa actatccgaa tcagccttta aatcgccata
    32641 agtgtgttcc tgccccaaaa cattatagac aggatagcta ggctgtgtct gagcaaaatg
    32701 ctcaatggtt tcaatcatat ctgctattgg tttatttgac acaataggga ttctccttca
    32761 agttaaaatt cattatagat aaagcttcct tgaccctgac caagatagct aaagaagtaa
    32821 agcagcccta gaaagataag aaaatacaag gctgtccgac caagaaagag gtacaattct
    32881 tttctctgtt tcatcaagaa aaaccattca tttctgtaat ttttcgctaa aataagagtg
    32941 attcttacta gcttattttt ctaccattgt accactttat atagtatctt ttcaattgtt
    33001 taccgtatgt ttccaataga tttcagctta ttttaaggat tatgcggttt ttctatatct
    33061 atttcaaaag aaaaaagcct gagattttca tctcaagctc tagcaataat agttattttc
    33121 taaactaaaa aagtgagaat acaagcacgg ccaaggctgc tccgataaca gggcctacaa
    33181 caggaatcca agcgtaagac cagtctccgt ctcccttgtt tggaattggc aagatgctgt
    33241 gcatgatacg aggtccaagg tcacgagctg ggttcaaggc ataacctgtt gtcccaccta
    33301 gtgatagacc gataccgaca atcaaagttc ccactgcaaa ggttccgata cctgcctgaa
    33361 agtcgtaaag acccaaagca aagattgtca acaccaaaac aaaagttcca aggatttcgc
    33421 taatcaagtt tgatacagta tccttgatgg ctggtccagt actgaaggtt gccaggatat
    33481 tgcctgcatt ttcttctgcc tcatagtgag gtttgaattg caaccaaacc aaaatctgac
    33541 ccagcatggc ccctgcgaac tgggctaaga tataaggcaa aacggaagcc caaggcaaac
    33601 caccttttaa ggccacaccg atggtcacag ctgggtttaa ataagctgga ctgagcttgc
    33661 cagatacaaa gactgcaacc gcaactgcaa tcccccaacc catagtaatc acaatccaac
    33721 ctgagctatt gctcttggtt ttaggaagaa ccacacctgc aacaacacca tttcctagaa
    33781 gaatcaggat taaagtccct agaaattctc caaataattc attcatcatc tttctgtctc
    33841 cattaaaaag aagggacggg cgacaaggaa tgctgccctc cacctctttt attttttctt
    33901 aattttttaa ttctgctaaa tcgttgttag cgagagctgc ttcgacatca gcacggtaag
    33961 ttgctttttc ttcttctgtc cagtcataga atcgtcccat ttcatccaaa attggctcaa
    34021 cgatactatc caagctatca cgcataaaga gcatgtgatt ggtacgacga agaaggaagt
    34081 caactgggct aagagtcaac tcattgcgca ttgcatagtg aagggacaag gtgtctgcca
    34141 agctaagacc tggcgcttgt tccaagctgt gagcaagtgc aaagaccttc ggtgcatttg
    34201 aaccgtaaag atttgctagg taatgagctt ccttgctatc caaaccacgt gaaactccaa
    34261 gttgcgcaaa ggcttcgatt tctgaatcca catttgctgg gttcaattct ccacctgaaa
    34321 cagggtaagt tttagaattg atcaatttaa agctacggtc aaattctgct ttgaggatgt
    34381 caaccacgcg ctccatagct ccttcagcca tcttacggta gtctgtgatt ttaccaccag
    34441 caagagtcaa gagaccattg tcatcacggt ccaagctaga cccacgagaa actgcagatg
    34501 gatccaaatg tttctcagat gtgctacttt caagcttgct gacagcagac tcaacatctt
    34561 cacgtgtttt ttctttggag agataagatt caacagtcgc aatcaagttg tcaaagcttt
    34621 catcactgat ggtaccgtta tttccaccat tatagtcaga ggcactgttc cctgcaatca
    34681 atggacgaag acctgcccag ctgctttcga tatcatcaat ggtgatgttg gattctggga
    34741 agcggttgtt gacaatgcca agtagataat ctacatcttc ttgagttact tttggatgct
    34801 ccaaatcacc tgtgtagtct gtatcagttg taccaaagta agtcttgttt tcacgtggga
    34861 gaacaaagac catacgaccg tcacccaaac ctgtgtcgaa gtaaactggc tgtgaaacct
    34921 tgattttact tgaatccact accaagtgaa ctcccttagt tgggcgcatt tgtgagaatt
    34981 gcgttccctt attagacaaa ttacgtactt tatcactcca aggacctgtt gtattaataa
    35041 ccagacgggc cttgatttca aatacttggt ctgtcaagag atcacgagct acaacacctg
    35101 taatcttgcc actttcgtca aagaggaagc cttctgcctt cacgtggttg gcaatgaggg
    35161 caccgtcttg gttggcacgt ttgatgtttt caatcacgag acgcgcatcg ttgttacgga
    35221 agtcaagata cactccacct cctaccaagc cttccttctt caagtttggc tggcgttcca
    35281 agacttcttt cttgctcaaa accttgttcg cagttggtgt gttgctcaca cctgctaaaa
    35341 ggtcgtacaa gtccatggct actttaagac ggaagaggct aaaggttgct ccatcttcat
    35401 cgtaaactgg taagagcatt ggatctggtt ttggaatgtg tggagcgatt tgttgaacca
    35461 ctgcacgttc agaaaccgta tctgagacca cttctacgtc gaattgtttg aggtagcgaa
    35521 gacctccgtg aaccaatttt gttgaacgac tagatgttcc ttctgcaaag tcttgcattt
    35581 caatcaaacc agtctcaaga ccgctagctg ccgcctgcaa ggctacacca gctcctgtga
    35641 ttcctccacc gataatcaag aggtccaggg tgcgttcctg cattttttta attgacaatt
    35701 cacgtgtttt ctttgaaaat tccatattta cacactttct aaataaatcg ctttattctg
    35761 ccagtattag tcgtctactt ccgcaaagac ttgagttgct ttcacagcct tcttccagcc
    35821 cttgtagagt tgttccttgc gagattcatt catagatggc tcaaagagtt ctcctgtctc
    35881 gttcaagagt ttcaactcgt ccaagtcttt ccagtaccct actgataaac ctgctaggaa
    35941 ggccgctcct agagctgttg tttccaagtt tttagcacgt gcaatatcaa tgcctaaaat
    36001 atccgcctgg aactgcatga ggaagttgtt catggctgca ccaccatcca ccttcaatac
    36061 ttgaatggca gtctgagtat ccacttgcat ggtgtcgatg atgtcacgca cttgataagc
    36121 aatagattgc aaagtcgcct tgataaagtc ttctttgctt gttccacgag tcaaaccaaa
    36181 gacggaacca cgagcatttt ggttccagta tggagcgcct agacctgtaa aggctggaac
    36241 gacataaact tcatcgttgt tgtgagaatc acgagcgtat ttttcagatt ctggtgaatt
    36301 ttcaaccatg cgaagaccgt cacgaagcca ctgaatagca cttcctgcga tgaagataga
    36361 accttccaag gcataataaa ccttaccgtt gattccgtaa ccaatggttg tcaagaggtt
    36421 gttttcagac aactgcatct cttccccagt attcatgatg atgaaagagc ctgttccata
    36481 agtattctta accatacctg gctcaaaagc caactgtcca aagagggctg cttgttggtc
    36541 cccagccata cctgagattg gcacctctcc accgtagaaa tggaatggag ctgtcttgcc
    36601 gtagatttcg gagttagaac gaacttctgg aagtatagcc ttcggaatgt taaggatttc
    36661 caaaatctca tcatcccatt tgagttcttt aatgttataa agcatggtac gagctgcatt
    36721 tgagtagtca gtcacgtgag ccgcaccgtc agtcaatttc caaaccaacc aagtatcgat
    36781 agtaccaaag agcaattccc ctttttctgc tcgctcttga gcgccttcta catgatccaa
    36841 aatccaacga accttggtag cagagaagta agcatcaata atcaaaccag tcttttcatg
    36901 gaatttttcc acataacctt ggctttttag ttgctcagcc aaaggtgctg tctggcgtga
    36961 ctgccaaacg atagcattgt agataggaag tcctgttttc ttatcccaga caaccgttgt
    37021 ttcacgttgg ttggtaatcc cgattgcctc gatttgattt ggcttgacgc cactttcgat
    37081 gaaagcaccc gcaataactg actgaacaga gttccaaatt tcattggcat tgtgctcaac
    37141 ccaacctgcc tgagggaaaa tctgggtaaa ctctttttga ctcgagctaa ccttttcccc
    37201 ttttttgttg aaaatgatgg cacgagaact tgtagttccc tggtcaatgg ccatgatgta
    37261 tttttcttgt gacatgtact gtcctcctga taaagatctt gaggtgtttc ctctttacac
    37321 ttactagtat acaaaataaa gcgctttctt gtttatcaac tttttttaat ttttaaaaaa
    37381 atgtgaggag attgtgtgaa tttcacaaaa aagacctgga ttaaccaagc cttttaagtg
    37441 aataataact gacgaatccc tgccaaatct tccatatcca agtcattttt taaataatag
    37501 actggggtct gttccttctt atgaatcggg ttattggtaa ccagcaaatc ataatgccga
    37561 ctagggtcat aagcttcaat ggtaataaaa cgattgtatt ccaaataccg tttcaaaatg
    37621 gctgccatcc ttgaaaagac aagaaaacca gatgtcaaat ccagaccaat ccgcatatgc
    37681 tggccaccgt tttcagccat atattcgatt aactggagcc attcccagag aatcttctta
    37741 tctaaatctg tcccctgttg aaaagcaggt agagcatgaa aaatctcctc tgctgtctct
    37801 ttaaaatcat gttccatcag taaatatgga tgacgattct ctaactggta cttgtagcga
    37861 tcctgtaaaa tatagccctt atagagatag acttgagcac aaagctgact gagttcatag
    37921 gtgacatggt cctctgtata atcccccaat agttcctcct tcttcatttc ttgaatcaat
    37981 tgcgtcagta aatctgccaa ctgccctcca aaaccaagaa tatattccat agtatgaaga
    38041 ggaagaatgc gatgagatag gagaaaagag aagaatatca tcatctcacc atcctcaact
    38101 cctagtggaa tatgttgccc agcaaaaaag gacggaacct ttctcagcaa acgaagatag
    38161 aagatattgt caaaataccc tcgcattttc tcttctatga cttgaaactg acaagcattg
    38221 acccgaagac gttgttgact gatgtgagcc cagagaacca agtccaattt ctgccccaca
    38281 gacaaacttg caccgcagat ttcctctaaa ttggcaatct cctgttttct ctctggtttc
    38341 tgcatgtgac cttcccattc ctgactcgac cagacctttc ggaaaagaca gaaatagaaa
    38401 tagcgaatct gatgctctgg acctcgccaa cgtccatttt ggatggataa atcaaattct
    38461 gacaaaatct gatttaaacc agccaagtga cgaccaagcg tagcctcgct aatcaccaat
    38521 tcttgagcca gctgatgggc taaaaactgt tggtggtaga gaagataaac caaaatctgg
    38581 tatttaacag cactctccaa aaacaagctc cgaatatctc tccccttggt agctgcaccg
    38641 atagacagac gcagattttc atcttctgag tgaatagcca gctctaagcc actatccaaa
    38701 gccttgtcat tgagcagggt gacatatttg gttagggttg cttttgaaaa tcctgtttct
    38761 tccattacag ccttgacggt cgtctgagac tcttgtaata gaaaggaaag gattgaaaat
    38821 tgaccacact cggctttctc catcaaatct cctaaataca tttgttaccc ctttcatttt
    38881 ctaccttaag catagcataa atcttacaaa tgctaaaata atctgtttct ctttttattt
    38941 tcatgctgat ttcctggtcc attatcctga aaatcagtaa acacacggct ccccctttgg
    39001 gcatttttat gctaaaatag tagctatgga taaaattatt aaaactatat cagaaagcgg
    39061 agcctttcgt gcttttgtcc ttgatagcac tgaaaccgtc cgcactgctc aagaaaaaca
    39121 tcaaacccaa gctagctcaa ctgtagcgct tggtcgaact cttatcgcca gccagattct
    39181 cgcagccaat gaaaaaggaa ataccaaact tacagttaag gtgttgggat ctagctctct
    39241 aggtgctatt atcaccgtcg ctgataccaa ggggaacgtc aaaggctacg ttcaaaatcc
    39301 tggtgttgac atcaaaaaga ctgcgactgg tgaagtccta gtcggacctt ttgttggaaa
    39361 tggtcaattc ctcgttatca cagactacgg tactggaaat ccttacaact ctataactcc
    39421 cctcatctct ggagaaatcg gtgaagacct tgccttttac cttactgaaa gccaacaaac
    39481 gccttcagcg gtcggcctca atgtcctttt ggacgaggaa gacaaggtca agattgcagg
    39541 tggtttccta gttcaagtct tgccaggagc caagaaagaa gagattgctc gctttgaaaa
    39601 acgcatccaa gaaatgccag ctatctctac tcttctcgaa agcgacgacc atatcgaagc
    39661 cctcctcaag gctatctacg gggacgaagc ctacaagcgt ctttctgaag aagaaatccg
    39721 tttccaatgt gactgtagcc atgaacgctt tatgaacgct cttgccagcc ttccaagctc
    39781 agacttacag gaaatgaaag aggaagacca cggggtagaa atcacttgtc aattctgcca
    39841 aactacttac aactttgatg aaaaggacct ggaggaactc attcgtgaca aatcttaata
    39901 caccttttat gattggcaat gttgagattc ccaatcgtac cgttttagcg cctatggctg
    39961 gcgtgaccaa ctcagccttt cgtaccatcg caaaagagct cggagctgga ctcgttgtaa
    40021 tggaaatggt ctctgacaag ggaatccaat acaacaacga aaaaaccctg cacatgcttc
    40081 atatcgatga gggcgaaaac cctgtctcta tccaactttt tggtagcgat gaagacagcc
    40141 tagcacgcgc agcagaattc atccaagaaa acaccaagac cgatatcgtc gatatcaaca
    40201 tgggctgccc tgtcaacaaa atcgtgaaga acgaagctgg tgctatgtgg ctcaaggatc
    40261 ctgacaagat ctactctatc atcaacaagg tccagtctgt ccttgatatc ccacttactg
    40321 tcaaaatgcg taccggctgg gcggacccat ctcttgcagt agaaaatgct ctcgctgctg
    40381 aagctgcagg tgtttctgcc ctcgccatgc atggccgtac ccgtgaacaa atgtatactg
    40441 gccacgcaga ccttgagacc ctttacaagg ttgcccaagc tctaaccaag attccattca
    40501 tcgccaacgg tgatatccgt actgtccaag aagccaagca acgcatcgaa gaagttggtg
    40561 ctgacgcagt catgattggc cgagctgcca tgggaaatcc ttacctcttc aaccaaatca
    40621 accattactt tgaaacagga gaaatcctac ctgatttgac ctttgaagac aagatgaaga
    40681 tcgcctacga acacttgaaa cgattgattg acctcaaagg agaaaacgtc gcagttcgtg
    40741 aattccgcgg cctcgctcct cactatctcc gtggaacatc tggcgctgcc aaactccgtg
    40801 gagccatttc gcaagctagc accctggcag agattgaagc cctcttgcaa ttggagaagg
    40861 cttaatagtt taaaacccgt aactctctta aagagcctct tgaatgccgc cattctttcc
    40921 tcatcaaaaa aactccgtag tctctagcgc tataaatcca gctagagatt acagagttat
    40981 tttttaatct aaacttccta caagggtata ttatctactg aaaggatatg gtatttgtag
    41041 ggaatacttt aaagagttct tacaggaagt cagtattaac tagttatatt aggtttagtt
    41101 tacccattca ccattggcat tgactctata gctatctaca gttgtgttga ctgcaagggc
    41161 acctgagcca ttgacatagt accatttatc tgatactttg aaccattggc ttgctttcat
    41221 agcacctgat gcttcaagat agtaccaggt atctccatct ttcacccaat ctgttgccat
    41281 tgaaccgtta gcgtttaggt agtaccatga accattgttt tggagccatc ctgtcgcca
//
LOCUS       DAVKJJ010000013        39197 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000013 DAVKJJ010000000
VERSION     DAVKJJ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39197
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.013"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(147..1814)
                     /locus_tag="VW144_000677"
     CDS             complement(147..1814)
                     /locus_tag="VW144_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEU9600159.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDAVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(1817..2182)
                     /locus_tag="VW144_000678"
     CDS             complement(1817..2182)
                     /locus_tag="VW144_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9600160.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(2339..2527)
                     /locus_tag="VW144_000679"
     CDS             complement(2339..2527)
                     /locus_tag="VW144_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU9600161.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(2643..2819)
                     /locus_tag="VW144_000680"
     CDS             complement(2643..2819)
                     /locus_tag="VW144_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU9600162.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     DGMWRAENVTQDLI"
     gene            complement(2930..3127)
                     /locus_tag="VW144_000681"
     CDS             complement(2930..3127)
                     /locus_tag="VW144_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU9600163.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNV"
     gene            complement(3555..5099)
                     /locus_tag="VW144_000682"
     CDS             complement(3555..5099)
                     /locus_tag="VW144_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEU9600164.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLELLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMSPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            5312..5614
                     /gene="gatC"
                     /locus_tag="VW144_000683"
     CDS             5312..5614
                     /gene="gatC"
                     /locus_tag="VW144_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU9600165.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGIDRDRLFKNVPEKDNYYIKVPAILDNGGDA"
     gene            5614..7080
                     /gene="gatA"
                     /locus_tag="VW144_000684"
     CDS             5614..7080
                     /gene="gatA"
                     /locus_tag="VW144_000684"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU9600166.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAHVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TIQSAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQVAAAFEATTDYHKQQPVIFGGDN"
     gene            7080..8522
                     /gene="gatB"
                     /locus_tag="VW144_000685"
     CDS             7080..8522
                     /gene="gatB"
                     /locus_tag="VW144_000685"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU9600167.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LDGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            8651..9211
                     /gene="efp"
                     /locus_tag="VW144_000686"
     CDS             8651..9211
                     /gene="efp"
                     /locus_tag="VW144_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU9600168.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            9233..9622
                     /locus_tag="VW144_000687"
     CDS             9233..9622
                     /locus_tag="VW144_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9600169.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            9615..10037
                     /gene="nusB"
                     /locus_tag="VW144_000688"
     CDS             9615..10037
                     /gene="nusB"
                     /locus_tag="VW144_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU9600170.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(10087..10323)
                     /locus_tag="VW144_000689"
     CDS             complement(10087..10323)
                     /locus_tag="VW144_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9600171.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(10336..10809)
                     /locus_tag="VW144_000690"
     CDS             complement(10336..10809)
                     /locus_tag="VW144_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600172.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIGLYVTIILGLGADVYSL
                     ILTMGLVGSFLLLIWRLKKKKMLFIFEKKSWNWSFVFYLFATYVVYQILGNFWARYAH
                     LINHRNIHDEYFTVLFSNGQPTFLSTILSFVLPVIIGPVFEETLDRGYFMNTFFP"
     gene            complement(10827..11012)
                     /gene="briC"
                     /locus_tag="VW144_000691"
     CDS             complement(10827..11012)
                     /gene="briC"
                     /locus_tag="VW144_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEU9600173.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            complement(11171..11938)
                     /locus_tag="VW144_000692"
     CDS             complement(11171..11938)
                     /locus_tag="VW144_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEU9600174.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEIGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(11935..12801)
                     /locus_tag="VW144_000693"
     CDS             complement(11935..12801)
                     /locus_tag="VW144_000693"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEU9600175.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(12838..14205)
                     /gene="accC"
                     /locus_tag="VW144_000694"
     CDS             complement(12838..14205)
                     /gene="accC"
                     /locus_tag="VW144_000694"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU9600176.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(14217..14639)
                     /gene="fabZ"
                     /locus_tag="VW144_000695"
     CDS             complement(14217..14639)
                     /gene="fabZ"
                     /locus_tag="VW144_000695"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU9600177.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            complement(14636..15121)
                     /locus_tag="VW144_000696"
     CDS             complement(14636..15121)
                     /locus_tag="VW144_000696"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU9600178.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVASEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(15124..16359)
                     /gene="fabF"
                     /locus_tag="VW144_000697"
     CDS             complement(15124..16359)
                     /gene="fabF"
                     /locus_tag="VW144_000697"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU9600179.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(16381..17112)
                     /gene="fabG"
                     /locus_tag="VW144_000698"
     CDS             complement(16381..17112)
                     /gene="fabG"
                     /locus_tag="VW144_000698"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU9600180.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVVAIDGGLSM"
     gene            complement(17146..18066)
                     /gene="fabD"
                     /locus_tag="VW144_000699"
     CDS             complement(17146..18066)
                     /gene="fabD"
                     /locus_tag="VW144_000699"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU9600181.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(18059..19033)
                     /gene="fabK"
                     /locus_tag="VW144_000700"
     CDS             complement(18059..19033)
                     /gene="fabK"
                     /locus_tag="VW144_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEU9600182.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            complement(19152..19376)
                     /locus_tag="VW144_000701"
     CDS             complement(19152..19376)
                     /locus_tag="VW144_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9600183.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(19436..20410)
                     /locus_tag="VW144_000702"
     CDS             complement(19436..20410)
                     /locus_tag="VW144_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEU9600184.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGKELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(20410..20844)
                     /locus_tag="VW144_000703"
     CDS             complement(20410..20844)
                     /locus_tag="VW144_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9600185.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(20938..21723)
                     /locus_tag="VW144_000704"
     CDS             complement(20938..21723)
                     /locus_tag="VW144_000704"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU9600186.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            22048..23412
                     /locus_tag="VW144_000705"
     CDS             22048..23412
                     /locus_tag="VW144_000705"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU9600187.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAKKALYNAFFGE
                     SKED"
     gene            23415..23786
                     /locus_tag="VW144_000706"
     CDS             23415..23786
                     /locus_tag="VW144_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEU9600188.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGCKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            24000..25274
                     /gene="serS"
                     /locus_tag="VW144_000707"
     CDS             24000..25274
                     /gene="serS"
                     /locus_tag="VW144_000707"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU9600189.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(25299..26198)
                     /locus_tag="VW144_000708"
     CDS             complement(25299..26198)
                     /locus_tag="VW144_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEU9600190.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(26308..26856)
                     /locus_tag="VW144_000709"
     CDS             complement(26308..26856)
                     /locus_tag="VW144_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU9600191.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(27074..28396)
                     /locus_tag="VW144_000710"
     CDS             complement(27074..28396)
                     /locus_tag="VW144_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEU9600192.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTIRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSIAESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMAIIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(28487..28575)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(28739..31075)
                     /locus_tag="VW144_000711"
     CDS             complement(28739..31075)
                     /locus_tag="VW144_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEU9600193.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEKLARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVHFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDIFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(31179..31727)
                     /locus_tag="VW144_000712"
     CDS             complement(31179..31727)
                     /locus_tag="VW144_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU9600194.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(31724..32026)
                     /gene="zapA"
                     /locus_tag="VW144_000713"
     CDS             complement(31724..32026)
                     /gene="zapA"
                     /locus_tag="VW144_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU9600195.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            32113..32994
                     /locus_tag="VW144_000714"
     CDS             32113..32994
                     /locus_tag="VW144_000714"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEU9600196.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEKKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            32998..33612
                     /gene="lepB"
                     /locus_tag="VW144_000715"
     CDS             32998..33612
                     /gene="lepB"
                     /locus_tag="VW144_000715"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU9600197.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            33692..33814
                     /locus_tag="VW144_000716"
     CDS             33692..33814
                     /locus_tag="VW144_000716"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorohydrolase"
                     /protein_id="HEU9600198.1"
                     /translation="MKIKEQTRKLAVGCLKQCFEVVDRTDEVSSKYCFEVADGS"
     gene            33891..36257
                     /locus_tag="VW144_000717"
     CDS             33891..36257
                     /locus_tag="VW144_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEU9600199.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLSKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTHLEKIYRQSKESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(36305..37588)
                     /locus_tag="VW144_000718"
     CDS             complement(36305..37588)
                     /locus_tag="VW144_000718"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU9600200.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     EDGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(37782..38141)
                     /locus_tag="VW144_000719"
     CDS             complement(37782..38141)
                     /locus_tag="VW144_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9600201.1"
                     /translation="MDSPKSLASYRVIGISDRVLEILKEYLLLEEGDYIFTNNGSPLM
                     ITCFNYFLKNRFRKSEIKKDDFVLTAHVFRYSHISLLAELEVPITAIMDRVDYTNETK
                     ILSVYTHVTEKMKSNIT"
     gene            complement(38440..39045)
                     /locus_tag="VW144_000720"
     CDS             complement(38440..39045)
                     /locus_tag="VW144_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU9600202.1"
                     /translation="MKNGKDVTYRGSANFIKDNVLITAAHNIYKHEFGKDAEELYVTP
                     ARTPNSVPFGRIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQ
                     KNLTGITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASN
                     RVVGVHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGW"
BASE COUNT        11478 a         8940 c         7477 g        11302 t
ORIGIN      
        1 caaaattttt caagataaaa gaagaaattt tctgaatttt ctattttaac gtttatttat
       61 gaatgttatt tttgttttta ttaaaaagat accgatttca tttactaaaa agaaaaaaga
      121 actgatttct cagtccttca ttaatcttat tccacactaa ataggtatgg gtaaacaggt
      181 tgttgacctt ggtgaatctc gacttcaacg tcttcgaatt cttctacgat ttcttgagcg
      241 atttcattgg caagttcttc gcttccgtct tcacctacat agaaggttac gatttcactg
      301 tcttcatcca acatatgttt caaggtttca gtcaatgttt ggtgcatatc agggtttgac
      361 acaagaattt ttccatccac catacctaaa ttatcgtttt catggatttc taagccatcg
      421 atcgttgtat cacgcacggc tgttgtgacg cttccgctaa cgacatcgct aagagcagct
      481 gtcatacgct cttggttttc ttcaatggac ttgcttggat caaaggcaag aagacttgtc
      541 ataccttgag gaagagtgcg agcctctacc actaccgctg gttgctccaa aacttctgcc
      601 gcagattgag ctgccatgaa gatgttcttg ttgtttggca agaagatgat gttacgggca
      661 ttaacctgtt caacagcctt gataaagtct tctgttgaag ggttcatggt ttgaccgcct
      721 tcgataacat aatccacgcc ttgagaacag aagatatctg ctagaccttt accagccacc
      781 acagcaatca aagcatactc tttttcttca gccgacttga taacttgagt agcttctttc
      841 tcaacctgtg cttcgtgttg gttacgcata ttgtcaactt ttaccttgac caagctacca
      901 tatttgagac cttcttgcat aacaagtcct ggatcttctg tatgaacatg gactttgaca
      961 atttcatcat cgttaacaac gaggagagaa tctccaagct catccaagta gttacggaat
     1021 tcatcgtagt caaaatcttt agcataggtt ggaccttgct taagagctac catgatttca
     1081 gtacagtaac caaacgtgat gtcctcagtc gctacgtgac cagctacaga cttatgatgc
     1141 tctacattga tcatctcact catgttggca ggagtcgcta caaagtcctc agatgcaata
     1201 tattcgccag taagggctga aaggaaacct tcgtagatga agaccaatcc ttgaccacct
     1261 gagtccacaa cgccaacttc tttcaatact ggaagcatgt ctggtgtttt agctagagct
     1321 gttttagcac cttccaaggc tgcgcgcatg acttcaacag cgtcatctgt ttgctcagct
     1381 tttttcttag caccgatagc agctccacga gaaactgtta aaatcgttcc ttcaacaggt
     1441 ttcatcactg ccttataggc aacttccaca cctgattgga aggccagagc caagtcttga
     1501 cctgttaact cgtctttatc cttgatagct tgggaaaatc cacggaaaag ctgagacgta
     1561 atcactcctg agttcccacg cgcacccatc aaaagccctt tggcaagaat gctcgctact
     1621 tctccaactg tagaagctgg cttgtctgca acttctttag caccattttc aatggtcatt
     1681 cccatatttg tcccagtatc tccatctgga actggaaaga cgtttaatga attgacatat
     1741 tcagcttgct tattcaagcg agttgatgca gcctgcacca tttcttgaaa taagctagta
     1801 gtaatttttg acacggttat tctcctacaa ctttgatatt ttgaatgtag acatttacag
     1861 tctgagcagt aattccaagc tggttttcca agctaaaggc aacacgctct tgaatgtttt
     1921 ttgacacttc actaatcttt gttccgtagc ttaacacggt atatacatca actgcaatac
     1981 tgccatcttc ggctgccttt acgacgacac ctttagaata attttcctta cctagcaggg
     2041 cttggaaatt atctttgagg gcatttttac tagccatacc gaccacacca aaaatctcag
     2101 ttgctgctcc acctacgacg gttgcaatca cttcatctgt tagttcgatt tgaccatctt
     2161 ttgtattaat ttttacagtc atccttttta cctcaactag ttgatactct attttatcat
     2221 atttcagccc aagtgtaaaa gcagaatgat gtatcagcgg atatttactc tatttttcaa
     2281 acgatgttat acccacaata aaagaaaaaa gaccctaagg tctcctttgc ttttattatt
     2341 aaacgcgttc aactttacct gatttcaaag cacgagctga agcccaaact tttttaggtt
     2401 taccatcgat aagaacagta actttttgaa ggtttggttt tacggcacgt tttgtttggt
     2461 tcatcgcgtg tgaacggttg tttcctgata cagtcttacg acctgtaaag taacatactt
     2521 tagccattgt gttttcctcc tattagatct aatatagcgg atgtgctagc accacatacc
     2581 gtactatgtt atcacacttt cttgtttttt gcaagggaat tggaagattt tttatttgtg
     2641 tcttaaatca ggtcttgcgt gacattttct gctctccaca tgccatcgtt gattaacaga
     2701 acaccagaat taaaattatg tgtataaaaa tcatctctaa ctgcagctaa gggtatagcc
     2761 gtcaagtcca aatcccacag ctcatctatc gattttctta caacaatatc tgaatccaaa
     2821 tacagtacac gagactcgct tacatacttt ggaataaaat acctaaaaaa gccgcatatg
     2881 aaagtccctc aaaggggaga cgataacctt tcagaatact actgtcaatc taaacattca
     2941 caatctcact attcaaagtc tctagtcttt tttccattaa ttggaaccat tctcgcggaa
     3001 ggtcatcatt aaaaacataa aacttaagat tataatgatg aacacaaaga gattttattg
     3061 ttgtttcaac tttatccata taagcattat ctgcacctaa gacaatcgct tttttctctt
     3121 ctttcacttt ttatctcatt tctttttatt cccatcatat gtttctacgt aaccattatt
     3181 ttcgcctatt cgttcgtaaa accataccag tggagatttt agatgaagtc ccattacggt
     3241 ttacaatttt tacattacga cacagagttt tacaaatcga tttcatttgc caaacgtagt
     3301 tagtaaggca gttagctagg ctacctactt tctgctacga cggaaaaatc cacaaatcaa
     3361 taccattcgt ggatttttcc tagtgaagcg tttaggtaag ccgccatagc ggttaccgat
     3421 tattagatta cgacacggag tttggcaaat cgattctatt tgccaaacgt agttagtgag
     3481 gcagttagct agttcgccaa atagcgacta gcgtccaaca atttggaact ttagttccaa
     3541 ttgttggtac tgagtcacat cttctcctct aactctacgt ctggatactt gtccgcaaac
     3601 cagcggaggg caaagtcatt ttcaaagaga aagactggtt ggtcaaaacg gtctttggct
     3661 aagatattgc gacttgacga catccgttca tccaagtcct caggcttgat ccaacgaacg
     3721 gtctttttac ccattgggct catgaccact tccgcattgt attcgccttc catacggtgt
     3781 ttaaagactt caaactggag ttgaccaaca gctcccagca tgtactcacc tgtttggtaa
     3841 ttcttataaa gctgaacggc tccttcttgc accaattgct caatcccctt gtggaaggat
     3901 ttttgcttca taacattctt agcagaaact ttcatgaaaa tttcaggagt aaaggttggc
     3961 aatggttcaa attcaaactt gttttttcca accgtcaagg tatccccaac ctgataagta
     4021 ccggtatcgt aaaccccgat aatatcacct gccacggcat tggtcacatt ctcacgactc
     4081 tccgccataa actgggtaac attagatagt ttagccccct taccagtacg agggagattg
     4141 acactcatgc cgcgctcaaa ttcgcctgat acgatacgga caaaggcaat acggtcacgg
     4201 tgacgaggat ccatgttggc ttggatttta aagacaaagc ctgagaaatc cttgtcataa
     4261 ggatccacaa tttcaccatc tgttttcttg tgaccatgtg gttctggagc aaacttgagg
     4321 aagatttcaa ggaaggtctg cacaccaaag tttgtcaggg ctgaaccgaa aaagacaggc
     4381 gtcaattctc cagccagaat agcttcctct gaaaactcat tcccagcttc atttaaaagc
     4441 tcaatgtcat ccttgacttg ctcgtagaaa ggattgctac caaaaagttt gtctccatct
     4501 tctaggctag caaaacgctc atccccttta taaagttcta aacgttggtt atagaggtca
     4561 tacaagccct caaaggcttt ccccatcccg ataggccagt tcatagggta gctagcaatg
     4621 cccaagattt cttccaattc ttgcaagagt tccaaaggct cacgaccgtc acggtccagc
     4681 ttgttcataa aggtaaagac tggaatgcca cgatgtttca caacctcaaa caatttcttg
     4741 gtttgagcct cgatcccctt ggcagagtcc acgaccatga ccgcagcatc caccgccatc
     4801 aaggtacgat aggtatcttc tgagaagtcc tcgtgccctg gcgtgtctaa gatattcacg
     4861 cgcttgccgt cgtagtcaaa ttgcataaca gatgaagtaa cagaaatccc acgttgcttc
     4921 tcgatatcca tccagtcaga tttagcaaaa gtccctgttt tcttcccttt taccgtacca
     4981 gcctcacgaa tctcaccccc aaagtagagt aactgctcag tgatggttgt tttccccgcg
     5041 tccgggtggg agataatggc aaaggtacga cgtttcttaa tttcttcttg aatattcata
     5101 agttctcttt ctttgattct ctatttttct tgtttcaata gctgagaatg atttttacat
     5161 tggattttac cattcctttc aatactccat tatatcggat tttagcattt ttttcaattt
     5221 ctatttcttt tcacttcccc ctcccttatt tataggaaaa tatggtaaaa tagaacagac
     5281 taaaaatcat catttcacga aaggatgcaa gatgaaaatt acgcaagaag aggtaacaca
     5341 cgttgccaat ctttcaaaat taagattctc tgaagaagaa actgctgcct ttgcgaccac
     5401 cttgtctaag attgttgaca tggttgaatt gctgggcgaa gttgacacaa ctggtgtcgc
     5461 acctactacg actatggctg accgcaagac tgtactccgc cctgatgtgg ccgaagaagg
     5521 aatagaccgt gatcgcttgt ttaaaaacgt acctgaaaaa gacaactact atatcaaggt
     5581 gccagctatc ctagacaatg gaggagatgc ctaatgactt ttaacaataa aactattgaa
     5641 gagttgcaca atctccttgt ctctaaggaa atttctgcaa cagaattgac ccaagcaaca
     5701 cttgaaaata tcaagtctcg tgaggaagcc ctcaattcat ttgtcaccat cgctgaggag
     5761 caagctcttg ttcaagctaa agccattgat gaagctggaa ttgatgctga caatgtcctt
     5821 tcaggaattc cacttgctgt taaggataac atctctacag acggtattct cacaactgct
     5881 gcctcaaaaa tgctctacaa ctatgagcca atctttgatg cgacagctgt tgccaatgca
     5941 aaaaccaagg gcatgattgt cgttggaaag accaacatgg acgaatttgc tatgggtggt
     6001 tcaggtgaaa cttcacacta cggagcaact aaaaacgctt gggaccacag caaggttcct
     6061 ggtgggtcat caagtggttc tgccgcagct gtagcctcag gacaagttcg cttgtcactt
     6121 ggttctgata ctggtggttc catccgccaa cctgctgcct tcaacggaat cgttggtctc
     6181 aaaccaacct acggaacagt ttcacgtttc ggtctcattg cctttggcag ctcattagac
     6241 cagattggac cttttgctcc tactgttaag gaaaatgccc tcttgctcaa cgctattgcc
     6301 agcgaagatg ctaaagactc tacttctgct catgtccgca tcgccgactt tacttcaaaa
     6361 atcggccaag acatcaaggg tatgaaaatc gctttgccta aggaatacct cggtgaagga
     6421 attaacccag aggttaagga aaccattcaa agtgccgcta aacactttga aaaacttggt
     6481 gctattgtcg aagaagtcag ccttcctcac tctaaatacg gagttgccgt atactacatc
     6541 atcgcttcat cagaagcttc atcaaacttg caacgctttg acggtatccg ttacggctat
     6601 cgcgcagaag atgcaaccaa ccttgatgaa atctatgtaa acagccgaag ccaaggcttt
     6661 ggtgaagagg taaaacgtcg tatcatgctg ggtactttca gtctttcatc aggttactat
     6721 gatgcctact acaaaaaggc tggtcaagtc cgtaccctca tcattcaaga tttcgaaaaa
     6781 gtcttcgcgg attacgattt gattttgggt ccaactgctc caagtgttgc ctatgacttg
     6841 gactcactca accatgaccc agttgccatg tacttagccg acctattgac catacctgta
     6901 aacttggcag gactgcctgg aatttcgatt cctgctggat tctctcaagg tctacctgtc
     6961 ggactccaat tgattggtcc caagtactct gaggaaacca tttaccaagt tgctgctgct
     7021 tttgaagcaa caacagacta ccacaaacaa caacccgtga tttttggagg tgacaactaa
     7081 tgaactttga aacagtcatc ggacttgaag tccacgtaga gctcaacacc aattcaaaaa
     7141 tcttctcacc tacttctgcc cacttcggaa atgatcaaaa tgccaacact aacgtgattg
     7201 actggtcttt cccaggagtt ctgccagttc tcaataaagg ggttgtcgat gccggaatca
     7261 aggctgctct tgccctcaac atggacatcc acaaaaagat gcactttgac cgcaagaact
     7321 acttctatcc tgataacccc aaagcctacc aaatttctca atttgatgaa ccaatcggat
     7381 ataatggctg gattgaagtc gaactagaag acggtacgac caagaaaatc ggtatcgaac
     7441 gtgcccacct agaggaagac gctggtaaaa acacccatgg tacagacggc tactcttatg
     7501 ttgacctcaa ccgccaaggg gttcccttga ttgagattgt atctgaggca gatatgcgtt
     7561 ctcctgaaga agcctatgct tatctgacag ccctcaagga agttatccag tacgctggca
     7621 tttctgacgt taagatggaa gaaggttcta tgcgtgtgga tgccaacatc tcccttcgcc
     7681 cttatggtca agaaaaattc ggtaccaaga ctgaattgaa gaacctcaac tccttctcaa
     7741 acgttcgtaa aggtcttgaa tacgaagtcc aacgccaggc tgaaattctt cgctcaggtg
     7801 gtcaaatccg ccaagaaaca cgccgttacg atgaagcgaa taaagcaacc attctcatgc
     7861 gtgtcaagga aggggctgca gactaccgct acttcccaga accagaccta cccctctttg
     7921 aaatttctga cgagtggatt gaggaaatgc ggactgagtt gccagagttt ccaaaagaac
     7981 gtcgtgcgcg ttatgtatct gaccttggtt tatcagacta cgatgctagt cagttgactg
     8041 ctaataaagt cacttctgat ttctttgaaa aagctgttgc cctagatggt gatgccaaac
     8101 aagtctctaa ctggctccaa ggggaagtcg ctcagttctt gaatgctgaa ggtaaaacac
     8161 tggaacaaat cgaattgaca ccagaaaact tggttgaaat gattgccatc atcgaagacg
     8221 gtactatttc atctaagatt gccaagaaag tctttgtcca tctagctaaa aatggcggtg
     8281 gcgcgcgtga atacgtggaa aaagcaggta tggttcaaat ttcagatcca gctatcttga
     8341 tcccaatcat ccaccaagtc tttgccgata acgaagctgc tgttgccgac ttcaagtcag
     8401 gcaaacgtaa cgccgacaag gcctttacag gattccttat gaaggcaacc aaaggccaag
     8461 ccaacccaca agttgccctt aaactacttg cacaggaatt ggcgaagttg aaagaaaact
     8521 agacagaaca aaaccagccc taaggttggt tttttcttct ctaccaactc ccaataacta
     8581 ttttggcttt atttccagag tattttatgg taaaatgaag agtaataata tttattaaag
     8641 aggtaaaaac atgattgaag caagtaaatt aaaagctggt atgacctttg aaacagctga
     8701 cggcaaattg attcgcgttt tggaagctag tcaccacaaa ccaggtaaag gaaacacgat
     8761 catgcgtatg aaattgcgtg atgtccgtac tggttctaca tttgacacaa gctaccgtcc
     8821 agaggaaaaa tttgaacaag ctattatcga gactgtccca gctcaatact tgtacaaaat
     8881 ggatgacaca gcatacttca tgaatacaga aacttacgac caatacgaaa tccctgtagt
     8941 caatgttgaa aacgaattgc tttacatcct tgaaaactct gatgtgaaaa tccaattcta
     9001 cggaactgaa gtgatcggtg tcaccgttcc tactactgtt gagttgacag ttgctgaaac
     9061 tcaaccatct atcaaaggtg ctactgttac aggttctggt aaaccagcaa cgatggaaac
     9121 tggacttgtc gtaaacgttc cagacttcat cgaagcagga caaaaactcg ttatcaacac
     9181 tgcagaagga acttacgttt ctcgtgccta atctctagaa agaggtcatt ctatgggaat
     9241 tgaagaacaa cttggcgaaa tcgttatcgc cccacgtgta cttgaaaaaa tcattgctat
     9301 cgctactgca aaggtagagg gtgttcactc tttttcaaac agatcagtgt ctgataccct
     9361 ttcaaaactt tcactcggcc gtggcattta tcttaaaaac gtggacgaag aactcacagc
     9421 agatatctat ctctaccttg agtacggagt aaaagttcct aaggtagcgg ttgctatcca
     9481 gaaagctgtc aaagatgccg tccgtaatat ggctgatgta gaactcgctg ctatcaatat
     9541 tcacgttgca ggtatcgtcc cagataaaac accaaaacca gaattgaaag atctatttga
     9601 cgaggacttc ctcaatgact agtccactat tagaatctag acgccaactc cgtaaatgcg
     9661 cttttcaagc tctcatgagc cttgagttcg gtacggatgt cgaaactgct tgtcgtttcg
     9721 cctatactca tgatcgtgaa gatacggatg tacaacttcc agcctttttg atagacctcg
     9781 tttctggtgt tcaagctaaa aaggaagaac tagataagca aatcactcag catttaaaag
     9841 caggttggac cattgaacgc ttaacgctcg tggagagaaa cctccttcgc ttgggagtct
     9901 ttgaaatcac ttcatttgac actcctcagc tggttgctgt taatgaagct atcgagcttg
     9961 caaaggactt ctccgatcaa aaatctgccc gttttatcaa tggactgctc agccagtttg
    10021 taacagaaga acaataaaaa gaaaaagtgt ttgatagata tcaaacactt ttttctttgc
    10081 ctcccactat ctaaaaaaat gataatagat ataattgtaa acaaaaatcc agataggttt
    10141 tgcatgattg agaaagttaa aaaaactatg gcagagaatc gttaatctca gattgtcggt
    10201 agaacgataa acaagggcaa aaaagaaacc aatcagacta taatataata aactaattgg
    10261 atctctgtga gatagtatca aatggctaat cccaaagatg atagcagata ggataacatc
    10321 caaatagtac ttggactagg gaaagaaggt attcataaaa taccctctat caagagtctc
    10381 ctcaaaaaca ggaccgatga ttacaggcag gacaaaagat aagatagtcg ataaaaaggt
    10441 tggttgtcca tttgaaaaaa gcacggtaaa atactcatca tgaatattcc tatgattaat
    10501 caaatgagca tagcgtgccc aaaaattacc gagaatctga taaaccacat aagttgcaaa
    10561 taagtagaag acaaatgacc agttccagct ctttttctca aagataaaga gcatcttttt
    10621 cttttttaac ctccaaatta atagaaggaa acttcccact aatcccattg ttaaaataag
    10681 agaatagaca tcagctccta accctaaaat gatcgtcaca tacaatccaa ttgtttgtgg
    10741 taaataggta gatagtaaaa taataagcaa aaatattcca aattgtctta gtttttttgt
    10801 gtttctcatc gtactttttt gaaagattac cctgctcgga agccgtactt ccaagcatct
    10861 atataagaat taagtgcccc ttgcctcata tagggagcaa attctctata atataaccat
    10921 ctactatatc catcttccca aacagcaaga ccacctgaag tttgctccaa gtcctcagtt
    10981 gaaagaactg taaatgtatt tgtacctgtc attgcaagta ccttcttaaa atagattgtt
    11041 gtaggctcac atttatagta tatttctttt tttgtctatt ttatagccca tctcctcaac
    11101 tggcaatttt tcgacctgaa ttacattttt ccataaaaaa tgagaccttt ctagtctcat
    11161 ttagtcattc ttagtatttt ctaaatcgtt gatagcgttc ttccagcaac tcttctagcg
    11221 gtttttgtga aagtctagcc agctccgttt ggagttcttt tttgacactc ttaatcagtt
    11281 ctttactaga aagtcctatt tcagaaatca ccttatccac cacgtccatt tctaacagtt
    11341 catgcgaagt gattttcatc agttctgctg cttccatggc gcgagtaccg tccttccata
    11401 aaatggaagc aaagccttct ggactgagaa tggcatagat agaattttcc agcatccaga
    11461 cacggtccgc gacagctaga gccagagccc cgcctgaacc accttcaccg ataataatgg
    11521 cgataatagg aactttcagg tcactcattt ccatgagatt gcgagcgata gcttcccctt
    11581 gaccacgttc ttctgctccg acaccaggat aagcacctgc tgtattaata aaggtcacaa
    11641 ctggacggcc aaatttctca gcctgtttca tcaaccgcag tgcctttcgg tagccttctg
    11701 gatgtggttg gccaaaattc cgtttgaggt tgtcttgcaa actcttgcct ttttggatac
    11761 caacaactgt tacagcttgg tctccaagcc aaccaatacc accaacaact gcaccatcat
    11821 cacgaaaaga acggtcacca tgtaattgga taaattcatc aaaaatgcct gtcgcaaagt
    11881 ccaaggttgt caagcgactc tgctcacgcg cttctctgac tatttttgca atattcatct
    11941 aggactccct ccatgcaatc tgactaggct agcaatcgta tctggtaagt ctcttctttt
    12001 gacaatagca tccacaaagc catgttctaa taggaattct gccttttgga aatcctcagg
    12061 caagctttca cgaaccgtat tttcaatcac acgacgccca gcaaaaccaa ccaagctctg
    12121 tggttcagcc agaatgatat cgccttccat agcgaaagaa gctgtcacac caccagtcgt
    12181 tggatctgtc aaaatggtca ggtaaaagag accagcattt gaatgacgtt taaccgccgc
    12241 agagatctta gccatctgca tgagactcat gattccttcc tgcatacggg ctccaccaga
    12301 ggctgtgaat aggacaactg gcaatttttc gacagtcgca tactcaaaca aacgagtgat
    12361 tttttcacct acaaccgtac ccatagaagc catgataaag ttagaatcca taatcccaag
    12421 agccacagtc tgacctttaa taagagcagt tcctgtcaca acggcttcat gcagacctgt
    12481 tttttcacgc atagatgcca gtttcttttg gtaaccaggg aaatgcaagg gatccttgct
    12541 ttcaatccct gtaaacaatt ctttgaaggt tcccatatca atcgtcaaag ccaagcgttc
    12601 ttgggcagaa atacgaaagg tatagctaca gtgcggacag atacgttcac ttcccagatc
    12661 cttctgatag atggtatgct tacagcctgg acactgggaa aataattcat ctggaacctc
    12721 tggcttagct tgaggttttt ccctaaccga acgattggga ttgattcgaa tatacttatc
    12781 ttttttacta aatagagcca ttgattcccc ttttcggttt aaactcttaa agtcatttta
    12841 ttctttttct tgatatttag gtaagaaggt ttccatcaag aaggaagtat cataatcccc
    12901 agcaatgaca ttgcgatctg aaatgaggtc aagctggaaa tctgcattgg tctgcactcc
    12961 ttcaatctct aattcataga gggcacgttg cattttcatc aaggcgtcaa aacgattttc
    13021 gccgtgtact atgattttgg caatcatact atcataataa ggcggaatgg tataacctgg
    13081 ataaactgct gaatccacgc gcaagccaac tccaccactt ggcagataga gattagtaat
    13141 cttacctgga cttggagcaa agttaaaggc tgggttttct gcattgatac gacactcgat
    13201 ggcatgaccg cgtaggacaa tatcttcttg cttaacagac aaaggctgac ctgccgcaat
    13261 gcaaatctgt tccttaacga tatcaacacc tgaaacaaac tctgttactg gatgttctac
    13321 ctgaacacga gtattcatct ccatgaaata gaaattgcta cttgcttcat caagaagaaa
    13381 ttcaatggtt cctgcattct catagccaac aaactctgcc gctcgaacag cagcagcacc
    13441 tatttcatga cgcagcgttt ttccgattgc aatcgaggga ctttcttcca aaaccttttg
    13501 gttattcctt tgaagagaac aatcccgctc acccaagtga atcacatgtc catgctcatc
    13561 acctaggatt tgaacctcaa tgtgccgagc tggatagata acccgttcta tgtacatggc
    13621 accattgcca taattggcct tggcctcact agaggcagtt tcaaaggcag aaacgaggtc
    13681 atctggtttt tcaaccttac gaatcccttt accacctcca cctgctgaag ccttgagcat
    13741 aacaggatag ccaatttttt cagcaacaat caaagcttct tcagagttat gcacttctcc
    13801 atctgaacct ggtataacag gcacacctgc tttaatcatc tgagcacgcg cattgatctt
    13861 atcccccatc atatccataa catgaccaga tggaccgata aacttgatac ctacttcttc
    13921 acacatggtc gcaaatttgg aattttcact gagaaatcca aaaccagggt gaatagcttc
    13981 tgcctcagtc aagactgcag ctgatagaac tgcattaata ttgagataag actctgttgc
    14041 cttgccagga ccaatacaaa ctgcttcatc tgccaaaagc gtatgaagag cttccttatc
    14101 agcagttgaa taaaccgcta ccgtcgcaat ccccaattca cgtgccgcac ggataatacg
    14161 aaccgcaatt tcaccacgat tggcaattaa aatttttcga aacatggaga acctccttag
    14221 ttcccaattg caaaagtaag gataccactg gctgcaagct tgccatccac ttcagccttt
    14281 gcttcaacca cagctatggt gccacgacgt tttacaaaag tcgctgtcat aaccaattgg
    14341 tcgcctggta caacttgctt cttgaactta accttgtcca taccagcgta aaagaccagt
    14401 tttcctttat tttcaggttt tgataactcc aacacaccag cagtttgcgc caaggcttcc
    14461 ataatcagaa cacctggcat aactgggtat tgaggaaagt ggccgttaaa gaaaggttcg
    14521 ttaatggtca catttttgat agcaacaatg gtatcctcgc tcacttccaa gacacggtcc
    14581 actagaagca taggataacg gtggggaaga gcttctttga ttccttgaat atcgatcatt
    14641 tgatacgtac caatccttta ccaaactcaa ccatttcttc gttagagacg agaatttccg
    14701 ttaccacacc atccttagga gctgggattt cattcatgac tttcatggct tcgataatta
    14761 ccaatgtttg accttttttg acactatcac caactgtaac gaaggcaggt ttatctggtc
    14821 cagcagccaa gtaaaccact ccaacaagtg gactctctac aagatttccc tcactagcca
    14881 cacttgcttc agctggagct ggaacttctt ctgctacagt ctctgctgga gcagatgtag
    14941 gagctactgg actcggtgtt gctagaacgg gtgctggagc gacttgagtt gcaacttcag
    15001 gcacaggtct cgcttcattc ttgctaaact gcaactcatc cgtcccattt ttataagaaa
    15061 attctctcaa acttgactgg tcaaattgag tcatcaagtc tttaatatcg tttaaattca
    15121 tacttatcta ttctcccaac gtttgaaagc aagaactgca ttgtggcctc caaaaccaaa
    15181 agtatttgaa atagcgtatg gaatttcttt ctccaagcct tgtccataaa cgacattagc
    15241 ttcgatataa tctgatactt cacttgtccc agctgtcatt ggtacaaagt tatgacgcat
    15301 agcttcgatg gtgacgatag cttctactgc acccgcagcc cccagcaaat gtcctgtaaa
    15361 agacttggtt gatgatacag gtacttcctt accaagaaca gctacgatag caccactttc
    15421 tcctttttca ttggcaggag ttgacgttcc gtgagcattg acataggcta cttgctctgg
    15481 agaaatctca gcttcttcca aggctagttt gatggccttg atagctccct gaccttctgg
    15541 atgtggagaa gtcatgtggt aggcatcaca agtatttccg taaccaacca cttcagccag
    15601 gatagtagct ccacgttttt cagcgtgttc aagactttct agaaccaaca tccctgaacc
    15661 ttcacccata acaaacccat tgcgatcctt atcaaatggg atcgaagcac gagttggatc
    15721 ctctgtagta gagagagctg ttaaggcttg gaaaccagcg atggcaaaag gtgtgataga
    15781 agcttctgtt cctcccacca acatcacatc ttggaaacca aacttaatgg agcggaaggc
    15841 atccccaatc gcatcatttg atgaagagca ggcagtattg atagatttac aaacaccgtt
    15901 tgcaccaaaa cgcatggcta cattcccaga agccatattt ggtaaagctt ttggaagagt
    15961 cattggtttg acacgtttgg gtcctttttc atgaaggcga agtacctgat cttcaatttc
    16021 cttgattcca ccaataccag atgcaacgat aacaccaaaa cgatccctat taagagcctc
    16081 tacatcaaga ttggcatgat ttacagcctc ttgggctgca tacaaggcat ataaagaata
    16141 gttatcaaaa cggttggtat ctttttttac aaagtattta tcgaacggaa aatcttggat
    16201 ttctgccgca ttatgcacat caaagtcact atgatcaaat tttgtaatgc caccaatgcc
    16261 gattttccca gttgctaaac tattccaaaa ttcttctggt gtatttccga ttggagatgt
    16321 tactccataa cctgttacca ctactcgatt tagtttcatt cttttcacct ctagctttcg
    16381 ctacatactt aagccaccat caatggcaac cacttgacca gttagataat cttggcctgc
    16441 taaaaatact gtcaaatctg caacctgctc tgcctgccca aattctttca tcggaatctg
    16501 agctagtgta gcttccttaa tcttatctga taagatagct gtcatatcag actcaatcat
    16561 tcctggagca atcacattga ctcgtatatt ccgactagcg acctcgcgtg ccacagactt
    16621 ggtaaagcca atcaagccag ccttagaagc agcatagtta gcttgaccaa tattccccat
    16681 caaaccaaca acactagaca tattaatgat agcaccttct ctggctttca tcatcggttt
    16741 caagactgat tgtgtcatat taaaggcacc agtcagattg accttgagca ctttttcaaa
    16801 atctgcttct gtcatcttga gcataagagt atcttgggta atccctgcat tgttgaccaa
    16861 aacatctact gaacccagtt ctgcaatagc ttgatcaatc atacgcttag cgtctgcaaa
    16921 atctgataca tctcctgaaa tgggaaccac cttgatacca tagtttgaaa actcagcgag
    16981 caattcttct gagattgccc cacgactgtt taagacaatg ttggctcctg cttgagcaaa
    17041 cttgtgggcg atggcaagac caattccacg actcgaacct gtaataaaga tatttttatg
    17101 ttctagtttc atttttttcc tttcaaaact tctacttatt ttagtctatt tttctaaaag
    17161 tgctactaaa ctcgcttgat cttccacatg agctaagtga gcagtttgat caattttttt
    17221 aacaaaacct gacaagactt tccccggtcc aatctcgata aagttgctta tgcctgcttc
    17281 ttgcatgacc ccaatacttt catagaaacg aacgggttcc ttgacctgac gcgtcaagag
    17341 ctgagcaatg tcctcttttt gcatcacagc agcttctgta ttgccgacta ggggacaagt
    17401 aaaatctgaa aaacttacct gagctagagt ttcagctagt ttctggctag caggctcaag
    17461 gagagcggtg tgaaagggcc ctgacacctt aagaggaatc aagcgtttgg cacctgcttc
    17521 ttgcaaaagt tcaaccgctc gatcaactgc aaccacttct ccagcaatga cgatttgtgc
    17581 aggtgtgtta tagttggctg gagtaaccac tccaagttca gaagcttttt gacaggcttc
    17641 ttcaatgacc tctactggcg tattgagaac tgctaccatc ttgccagagt cagcaggagc
    17701 cgcttcttcc atataggctc cacgcttagc taccaaggca accgcatctt caaaatccaa
    17761 ggcgccgctt gccaccaagg cagagtattc tccaagagac aaaccagcaa ccatatcagg
    17821 ctgatagccc ttttcttgca ataaacggta gatagcaacc gaagtcgcta gaatggctgg
    17881 ttgcgtatag cgggtctgat tgagtttgtc ttcttccgta tcgatgagat aacgtaaatc
    17941 ataaccgagc acctgactcg ctcgatcaat cgtttctttg acaatcggat actgatcata
    18001 gaaatcccgt cccatcccta gatactgggc accttgacca gcaaataaaa aggctgtttt
    18061 agtcatttct tacaactcct gcccagcgag aggcttcttc ttgaattttc ttagcggctc
    18121 cgtaatacaa atcttttagg atttcttcag ctgtttcttc tttagaaaca agccctgcga
    18181 tttgacctgc catgacagag ccaccatcca catcaccgtg aacaactgct ttggctaggg
    18241 cacctgctcc catttgttca aagatttcta aatcaggatc ttcctgctta aaggcatctt
    18301 tttcagccag ttcaaaatct ctagtcaact gatttttaat agcacgaaca gcatgaccaa
    18361 agtgctgagc tgaaatcgta gtatcaatat cccttgcttt taaaattttc tccttgtagt
    18421 ttggatgggc attcgactct tttgcaacta caaaccgtgt ccccacctgt acagcctctg
    18481 cacctagcat aaagccagcc gcagcacctt caccatccgc aattcctcct gcagcaataa
    18541 caggaataga tatagctgtg gctacctgtc gcaccaaggt catggttgtt aatttaccga
    18601 tatgcccccc agcttccatt ccttctgcaa taacagcgtc tgcaccgatt ttttccatgc
    18661 gtttagctaa agcgacacta ggaacaacag gaataacgat tatcccagct tcatggaaac
    18721 gttccatata cttgcttgga tttcctgctc ctgttgtgac aactttaaca ccttcttcaa
    18781 taacgagatc cacgatatct tccacaaagg gagataagag catgatgttg accccaaagg
    18841 gtttatcagt caatgatttg attttatcaa tattggcctt gacaacttct ttcggggcat
    18901 ttcccccacc gataattcct aatcctccag ccttggaaac agcccctgcc aaatcaccat
    18961 cagcaaccca ggccatccct ccttggaaaa taggatagtc aatcttcaat aattctgtaa
    19021 tacgcgtttt catagtgcct ccaaccttcc ttgcttacgt aatagttcga tttcaccata
    19081 atttgacagt caaactatta cctaaacaag agggagtggg tttctcccta ctccttctac
    19141 taatattctg cttattttgc ttgctcttca acgtaagcaa ccaagtcacc aactgttttc
    19201 aagtcatttt ctgcttcgat ttggatatca aaagcatctt cgatttctga gattacttgg
    19261 aacaagtcca atgaatctgc gtccaaatca tcaaaagttg attcaagtgt tacttctgat
    19321 gcgtcttttc caagttcttc aacgataatt tcttgtactt tttcaaatac tgccatgata
    19381 ggactccttt aaaataaata gtttttttat aacaatgtgt tcaccacatg attacctaaa
    19441 ttgtaagaat gagcgtgccc caggtcaagc ctccaccgaa gcctgataga agaacagtct
    19501 ggctaccatc taaagggatg agaccttgtt ctacacactc tgaaagtaaa atcgggatac
    19561 tggctgcact ggtattgcca tattccatca tattggctgg aagtttggct cggtcaacac
    19621 caatttttct agccatctta tccaaaatac ggtcattggc ttgatgaagt agcagataat
    19681 ccaagtctgt cacctctata ggagattcat caatagtctg cttgatagac ttggctacat
    19741 ctcgaatggc aaaatcaaag actgtgcgtc catccatctt caaaaacgaa tctgcacttt
    19801 cttgatctga aaatggagaa tgcaaacctg aatgcccata agttaaacac tcgctgcgac
    19861 ttccatcgct attgagactc tcagctaaga aatgctcttg ctcgctagct tctaacaaga
    19921 caccaccagc accatctcca aacaacacag ctgttgatcg atccgaccaa tcgactgcct
    19981 tagagagggt ttcactacca atcaccaagc ctttttgaaa gcgaccagaa gcgataaact
    20041 tttcagcagt tgaaagagca aatacaaatc cactgcaagc cgcggttaag tcaaaagcaa
    20101 aggctttatt agcgccaata ttagcttgaa cacgagcagc tgtagagggc atcatcgaat
    20161 ctggagtaat ggtagctagg atgataaaat ccagttcttt tcctgttatt ccagcttttg
    20221 ccatcagttt cttagcaacc tctgtagcca aatcactggt agattctgtt cttgaaatat
    20281 gcctttgtcg tattcccgtt cgacttgaaa tccactcatc attggtatcc ataatctgag
    20341 ccaagtcgtg atttgtaacc acttgctctg gcacataatg agcaacctga cttatttttg
    20401 caaaagccat tatttcaaat cctccaaaaa ttggtaaaga ttagtcaagc ctttacccat
    20461 gacagcaatt tcttcctcgc tcatgccatc aataattttt tctaccatgg ccttgtggaa
    20521 gcgtttatgc agtctatgaa tcaagcgacc cttctttgtc aaatgcagat gcaccacacg
    20581 acgatcctgt tctgaccgaa ctcgctcaat gtagccctta cgctctaaat tgttcaaact
    20641 tgtcgtaaca gttccaagag ttaccatcaa ctcttttgac acttgacttg gagtcacatc
    20701 tggagcctta ccgatgacat caatcgtatg catttctttg atggagatat ccttaaaacg
    20761 actacctctc aagttcactt cctcaattac aaggacattg ttaaatatag atgttaaata
    20821 ttcattgatt cgttggtagt ccatttttca tatccctcct tcttcaaaaa acttttacaa
    20881 tcaaaacatt tgacagaaag aattatatca aaattcaaag aatgatgcaa gtatttttta
    20941 ttttcctgta aatttaggtc ttcttctctc cgaatgagct cgcactcctt ctttgaaatc
    21001 ctctgtttga gctaaggatt tctgtaggtt cagttctaaa gtagcgtaac cctgccagtc
    21061 tttaaactgg ctctcccaaa ccaacttctt aatggcagca taggaattac ttgaagcacg
    21121 tcttaatttt ttaagaagct gttctctcgt cttttcaagt ttttcagctt cagaaacgcg
    21181 gtaaaccaac ccccattcta gggctttttc tgccgttaaa gcctctcctg tcatagctaa
    21241 ttgagcagca cgcgtcacac cgatactgcg gctcaagaga tgaattcccc ctgcatctgg
    21301 agccaaacca acaccaacaa aggcttggat aaacttagcc ttatccgtcg ccaaacagaa
    21361 atctgccgca acagccatat tcgctgcggc acctgcaaca gcaccgtcaa cttccattaa
    21421 aacaggtttg gctatttgct tgattttata agaaatcgta ttgaccaact ctgcgatttt
    21481 tgtcaatgat ggaatatcat cctcatccac tgcccgcttc atctctacca aatctccccc
    21541 aactgagaag acctttccat tggcattgat taagataaaa tgcacagctg gattttcttc
    21601 tgccaaagtc agagcttcta aaatctcctc acacatggga atatgaaaac catttgcgac
    21661 ctcaggacgg ttcaaggtaa ggattgccaa gtcctcttca agctgataaa taatgtgttc
    21721 catcagggct cctttttatt ttataagcag tttatactct tcgaaaatct ctttaaaccg
    21781 cgtcagcttc catctgcaac ctcaaaacag tattttgagc tgacttcggc tagcttccta
    21841 gtttgctctt tgattttcat tgagtgttaa attatttcac tgtcaaagta tatctttttt
    21901 aggagcagaa agcaagaaaa aatcgactga aagtgtctca gccgataaga aaggctcgct
    21961 ttaggctaga aagcatctta ttttcttcat tgaaaaaagc ctttctttct gctataatcg
    22021 tataaaatac ttactttagg agttcttatg aaagttgtta aatttggagg tagttctctt
    22081 gcctctgcta gtcaattaga aaaagtttta aacatcgtca aaagcgattc agagcgtcgt
    22141 tttgtagtcg tttctgcgcc tggtaaacgc aatgctgaag atactaaggt tacggatgcc
    22201 ctgattaaat actaccgcga ctatgttgcg ggtaacgata ttagcaagag ccaaagctgg
    22261 attatcgacc gctatgctgc tatggttagt gaattgggac taaaaccagc tgtgctagaa
    22321 aaaatttcta aaagcattca cgccttggcc actcttccta ttgaagaaaa tgaatttctc
    22381 tacgatactt tcctagcagc cggtgaaaat aacaatgcca aattgattgc tgcctacttt
    22441 aaccaaaatg gtatcgatgc acgctatatg caccctagag aagctgggat tgtggtcaca
    22501 agtgaacctg gtcacgctcg catcattcca tcaagttatg acaagattga agaattgaca
    22561 aacaccaatg aagtccttgt cattcctggt ttctttggtg tcactaagga aaatcaaatc
    22621 tgtactttct cacgtggagg ttctgatatt acaggttcta tcattgctgc tggtgtcaaa
    22681 gctgacctct atgaaaactt tacggacgtt gatggtatct ttgcagccca ccctggtatt
    22741 atccaccaac cacactcgat tcctgagttg acctaccgtg aaatgcgcga gttggcctat
    22801 gcaggcttct cagtccttca tgacgaggct cttcttcctg cctaccgtgg aaaaattcct
    22861 ctggttatca agaataccaa caaccctgac catccaggta ctcgtatcgt tctaaaacac
    22921 agtaatgatg aatttccagt tgtgggaatt gctggtgact caggctttgt cagcattaac
    22981 atgtcgaaat acctcatgaa ccgtgaggtt ggatttggcc gcaaggttct gcaaatcctg
    23041 gaagaactta acatcggttg ggaacatatg ccaacaggta tcgacgatct ttctatcatt
    23101 ctccgttctc gccaactaac tcccatcaag gaagaagaaa tcctgcgtca gttggttcaa
    23161 aaggctaaag tagatcatgc agaaatcgaa cacgaccttt ctatcattat gattgttggt
    23221 gaaaaaatga agagccacat cggagtgact gctactgcga cacgcgctct atctgaaaac
    23281 aaaatcaaca tccagatgat gtctcaaggt tctagtgaag tttctattat gtttgttgtc
    23341 aataaagacc aagagaaagc agcaaaaaaa gccctctaca atgccttttt tggagaaagt
    23401 aaggaagact aagcatggcc caatcactta acaaaacagt actcctcagt acgacaggca
    23461 cttcctacct ctctatcgct gggaaagttg ggaaattcct tgtcggagat caggctttgg
    23521 aattttaccc agatgtcaat gtcgaacaat ttatccagat tccttggagc catatcaatc
    23581 agattggagc caatgtcact ggatgcaaaa tcagccgcca cttcgaagtc tttacagaca
    23641 aaggaaaatt cctctttgct tcaaaagact caggtgccat tctcaaaatt gcacgcgaaa
    23701 aactaggcaa tgacaaggtc gtcaaacttc cgaccctgat tcagacaatt agccaaaaat
    23761 ttaaaaatct atttgcaaaa aagtagaaac tttagtataa tcatagcaac ggataagtag
    23821 gttatcttct tctttcagaa agtctgcggg tactgcgagc agataggagg gataggtgaa
    23881 attctaccgt tagtaacaat tacatacaca atcaagtgca cacctgtgaa gttggatgga
    23941 accgtggccc tgccactcca acgttttgtc aggcgtgctt ttttcataaa ggagttctta
    24001 tgttagatat caaacgtatt cgtacagatt ttgaagctgt cgcagaaaaa ttagctacac
    24061 gtggtgtaga tgctgctgtc ttgaatgaaa tgaaagaaat cgatgctaaa cgtcgtaaca
    24121 tcttggtcaa ggttgaaact ctcaaagcag aacgtaacac agtttctgct gagattgccc
    24181 aagctaagcg caacaaggaa aatacagatg acaagattgc tgccatgcaa aatctatctg
    24241 ctgaggttaa agccttggat gctgaattgg cagaaatcga tgctaaattg acagaattta
    24301 caacgactct tccaaatatc ccagctgaca gcgttcctgt tggggctgac gaagacgaca
    24361 atgtggaagt tcgccgttgg ggtactccac gcgagtttga cttcgaacct aaagctcact
    24421 gggatctcgg tgaagacctt ggtatccttg actgggaacg cggtggtaag gtaacaggcg
    24481 ctcgcttcct cttctataaa ggcctcggtg ctcgtttgga acgtgctatc tacaacttta
    24541 tgttggatga acatggaaaa gaaggctata ctgaagtcat cacaccttac atagtcaacc
    24601 atgattctat gtttggtact ggtcagtatc caaaatttaa ggaagatact tttgaactca
    24661 gcgataccaa ctttgtcttg attccaactg ctgaagttcc tctgacaaac tactaccgtg
    24721 atgaaatctt agacggcaaa gatcttccaa tctacttcac tgctatgagt ccgtcattcc
    24781 gttctgaggc tggttctgcc ggtcgtgata cgcgtggctt gatccgtttg caccaattcc
    24841 acaaggttga aatggtcaaa tttgccaaac cagaagaatc ttacgaagaa ttggaaaaaa
    24901 tgacagccaa cgctgaaaac attcttcaaa aactcaacct tccataccgt gtcgttgctc
    24961 tctctactgg agatatgggc ttctcagctg cgaagactta cgacttggaa gtgtggattc
    25021 cagcacaaaa caattaccgt gaaatctcaa gctgttcaaa cacagaagat ttccaagccc
    25081 gtcgtgccca aatccgttac cgtgatgaag cagatggcaa ggtgaaactc cttcatacct
    25141 tgaacggttc tggacttgca gttggacgta cagtggctgc aattcttgaa aattaccaaa
    25201 atgaagatgg ttctgtgacc atcccagaag cacttcgtcc atacatgggt ggagctgaag
    25261 tcatcaaacc ataaaaaata aggtttagct atttctagct agaccttttt tcgtaaccaa
    25321 atcagataag cacctagtac aaagaataaa atagttaggc atataatggt ttcagccaat
    25381 accaggtaat ccagaaatgg aagtttcaaa attccctgag ccatcttgag cgaggtcgct
    25441 gtgataatgg ttgggaaggt gagggctgag aaggctggtt gaaaaccttg ttttaaaatg
    25501 ttgggcagac gagttaaaac aaagaaaaag aaggattgag aagccaaaat catgacaatc
    25561 aagacccaag tcggcaggct ggttcctcct actcgaacta gagaagccaa gagtagagag
    25621 aaaggagcac agtagattcc ttcttgtcca agcaaggcta gtgggagtgg atgtttcttt
    25681 aaatcgctat aaataagggg atagagatag aaggtcaaga gaaaaccaaa actcaaggtc
    25741 gcataggcaa tttcgataat acctaccaga ggataggtca aggcagccac tgctatcccc
    25801 acatagagaa ccgtccagct tggagtggca tgaaccctcc gccctggaca agcaaacttg
    25861 atggtaaaac cagcaatcaa ggtcaaatcc aagagaaatg aaaaccacca aatcccttgt
    25921 gctaccaaag gaagataaga gaatacgcga aagacatagg tcgataaaat catcccagcc
    25981 ataggaaagg ttgccattcc tgacaaaaga gggggcttgg tcaattcttg cttggtttct
    26041 ttccaattaa agagatgcag aattagaaag taaatccata aaaccaaacc agtcagacta
    26101 aaaagatggg atagaaccgg caacgtatct aaaataagat ttccagctcc tgccaaacct
    26161 agcaaacaac ctgaaaatac taaggggagt tttttcatcc taacctccaa taatcatgtt
    26221 agtttcagta taacataaaa gcgcttaaat gaggatttaa aaaaacgaag tccgcttatt
    26281 tcagacttca ttttactcag atatgaatta ggcataaggt tgcaattctg gattaattgg
    26341 tgtattagct aagttgttgg catagttaca gaggattgct aggctgacac caaaaaccac
    26401 atccaaggca ttttgttgag tgtagccagc ttctaaaaac tcagacaagg cttcatctcc
    26461 tacacgaccc ttggtattga taactgccaa ggtaaactta gctagggtat ccaatttagg
    26521 atctgtttca attggagtac gattgcgaag agcttgaatc aagtcatcat tcatctggat
    26581 ttgtttgatg gaaaaggctg tgtgacctgc gacacagaag gcacaaccat tggtcacggc
    26641 tgccgtgatt tgcaccactt cacgctcaac gggtgtcagg ctgttgcgac ggtggataga
    26701 tgagacaatt tggtaggctt ctaaaacagt cggggcattg gccaagagac cgattaggtt
    26761 gggaatatag ccattgttgt ctttttctac tgtttcaaga atttctttca cttctgctgg
    26821 tgctgactct actgtatgga tagtaaatgt tgtcataaga tacctctttt cttattattg
    26881 acactaatat tattggaaaa tcttataaaa tcctgattcc taagtttatc taagataaag
    26941 ctttattctc tcataagatt ttcgttgtta tattagttta tcacacttcc aatcacttgc
    27001 ataatatata ttatatatca ggctgataaa aattatttat aggcaaaaaa atcacacgag
    27061 ctgtgtgatt ccattatttg tcaaaatact ttttagtttc agcaataacg actggcgaca
    27121 agaccaagag ggcaatcaag tttggcagag ccatcaaggc gttaacgata tctgcgataa
    27181 tccagaccat atccaactcg ataaatcctc ctaacaagac catgagcaca aaaaccacac
    27241 ggtagagcca gataaagcga accccaaaga ggaactcaaa acagcgttct ccgtaatagt
    27301 tccaacctag aatcgttgta aaggcaaaaa gtacaaggaa gatggtcaag agggcaggcc
    27361 caaagtgtga aaagactgtt gagaaagctg actgagtcaa ggcaacccca ttcaagtcac
    27421 cactccaaac tccagttacc aagatggtca aaccagttag agtacaaatg atgagggtat
    27481 caataaaggt tcctgtcatg gaaatcaaac cttgctctac tggttcattt gtcttggcag
    27541 ctgcagctgc aataggagca gaacccagac cagattcgtt tgagaacaca ccacgcgcca
    27601 caccattttg aatagccatc cgaacgctag caccagcaaa tccacctacc gcagcaaggg
    27661 gactaaaagc tgaggtaaag actaaagcga ttgtgccagg gatttttcca atattaaaga
    27721 aaataactgt aagagttcct aagatataaa tgatggccat aaaaggaaca acagtagttg
    27781 aaaccttaga aatagacttg agtccaccaa agactgcaat cgctacaaag acagacaaga
    27841 cgagagctgt gatggctggc gaaatcgtcg ttgtattttg gatagattct gcaatcgagt
    27901 tgacttgggt gaaggttccg attcccaaga gagcaaccaa tactcctgct actgcaaaca
    27961 aaacagcaag tggtcgccac ttttctccca tccctagaag gatataatgc atgggacctc
    28021 ccgctactgc accatggtcg tccttggtgc ggtatttgat ggccaagagt ccttccgcat
    28081 acttggtagc cattccaaag aaagccgcca tccacatcca aaatagagct cctggtccac
    28141 caaccttgat agccgtcgca actcctatga tatttcctgt tccaacagtt gatgccaagg
    28201 ctgtacacag agctgcaaaa ctggatacat caccatgtcc cttatcctgg ataaaaataa
    28261 gctgaaaagc cttgggcaga cgcaaaacct gcaagagtcc tagccgaata gttaggtaaa
    28321 tccctgttcc gaccaataaa atcaagaggg gcggtcccca agcaaaagca tcgattgatt
    28381 taagcaattc taacatttcc ttctcctatc gtttcaaccc caaaagaaag agcacatgca
    28441 agatacatgt actctggaat gcttagataa atgctaaaaa gcggtctatc ctagctctgt
    28501 ccttttacct gagagtttga gcagttgcct gccttgcccc ttcggtgcct ttacggtctc
    28561 tccagagttc cgtccattta cagtcatgga aaatcaaacg attccccact tctattaaac
    28621 ttcattcggt gttggtattt aattgattct aattttacaa aaaatgttgg cttttgtcaa
    28681 tgtgtttatt agtaaaaatt agttcaacag tttttacttt ataaagtcca gaatactgct
    28741 atcctttaaa agtgacaata gtcgcaccac tgcctccagc attttgtggg gcatagccga
    28801 aactcttgac atgtttgttt ctttgcaagt atttggtaac tccttcacgg atgactcctg
    28861 ttccgatacc atggatgata tcaacttgag ccatattgtt aagcaaggct tggtcgatga
    28921 agatatctag ctcattcatg gcttcttcat agcgcttgcc tcgaagatcc agtctagctt
    28981 gaggtcctcg cccagaagtt cgtttcacaa cattgacctg cttcttcttg actggttttt
    29041 cttgctgggc ttgaacaaga tcaaactctt tctcttccaa ggtcatctta atcaagccaa
    29101 cttgggcttc ccagcggccg tccttgagtt gactggtcaa ggtaccacgc tgaccataac
    29161 tgagaaccac gatatcatct cccacctttg gagctcgttt tttcttggcc ttttgaagga
    29221 ccttgttttt agacaagtcc actttttcag gagccaattt tttcaacttg gccttggctt
    29281 caatgatttc gtggggcttg agttgggatt tactgtggag atttttgaga atctggtcac
    29341 tttcacttag ggccatatcc acaatctcag cagcctgttc acgcgccttg ttaagctcgg
    29401 tttccttttc acgattaagc tcgttgtaga gtttttttag cgcacggttc atcttgagat
    29461 tttcttgctc cacctcacgg atattgtcca aacgtttgcg gctttccagc gtctgctctt
    29521 ctaattgctc aatgatacga ttgacgtcat tgtcctgatc gatctgctga ctggcatctc
    29581 ctacgataac ttcagatagg cctagacgtt tggcaatttc aaaggcatta cttcggccag
    29641 gaacaccctg cataaagcga taggtcgggc gaagagttgc agtatcaaac tccatactgg
    29701 cattttgcac aaaggctgtc tcaataccgt aggccttgag ttctggatag tgggtcgtcg
    29761 ccatggtctt gatttgacgc aggcgaaggt cctccagaat agccatggca agggctgctc
    29821 cctcttgggg atcagtacca gcccccagct catccaaaag taagagtgaa tgttggttga
    29881 ccttgccaag aatatccacg atattggtca tatggctaga gaaggtagac aagctctgct
    29941 caatagactg ctcatctcca atatcagcaa agatttcttc aaaaatacca acacgacttc
    30001 ccttgtctgc taaaatcggc aatcctgact gggccatgac ctgtgtcaag cccagagttt
    30061 taagcatgat ggtcttccca cctgtattgg gacctgtaat gacaatagct gttaaatctt
    30121 gaccaaaatg gacatcattt gcgacggcat ttttgaccaa aggatggcag acatggagca
    30181 gttgaatctc ttgattttct gacagctgag gcacgactgc ttgtctttct tggataaatc
    30241 gaaccttggc acgaatcaag tccagatgac cgataatcca agcgtcatta gcaatctcag
    30301 ccgcatgagg gcggacacgc tcagaaattt cttggagaat gcgaagcatt tcatagcgct
    30361 catctgctcg cagactagca atttcttcgc tcagtttgac tacctcacgt ggttcgatat
    30421 agacggtgtt tccactagca gaaatatcat gaacgacacc tgcaatctta ttgcggtagg
    30481 tgtttttgac tggtaaaacc tgacggccat ttctgctagc aacaattcct tctgtcaaca
    30541 tctgcgcttt ttgcttgagc aagtcttgta aaacatcgcg tacctgactc tcgctatcat
    30601 gtatttttcg acggattcgc gccaattttt cactggcaaa attttcaatg aaacccgcat
    30661 cattaaaggc ctgaagattt ccttgtaatt gcggaaaatc atgtaatttc tcaaaccaaa
    30721 gggctaattc ttccaagctg acattttcca gattggcgta aaaactttga agttctcggc
    30781 tggcaagaag cacgcgtttc aagagtagga actcctcgat attgagatcc gctcccatct
    30841 ccaacctctt gcagactcct gcaatttcct tagttgagag aatagtaaaa tgcggttgct
    30901 cgacgaaaag agcctgcatt tccttcatct cagcaaaagc ctgtttgatt ttatctgctt
    30961 tggcagtcgg agccagttgt ctcaattgct ccaagccctg ctcggtcaac aaatgaggct
    31021 caaacaaggc cttgacctta tcgaactcta atgtttctaa tattttctta ttcatcttta
    31081 tttccttgtc tttgaatgtc taggtgtggt agctttttac attctgatag attcgagtca
    31141 aatctgtaaa caaagggcta ggaaaactcc tgcccttttt atccgattaa atttgtcacc
    31201 cagatttgtt tgagccaact ggttgttatc ggtatgctct ggatgatgtg ttttgcgacg
    31261 atactctttt caagaggatt ttgtataact gccatgggga tggtcgccaa gattgtcaag
    31321 gccatttgca agacaaataa ggtcaccaac atggacaaga tacctgctga aacttggaac
    31381 aacttaccac ccagtttttt gctaggaatc aagtgtaaga gaagaccaag caaacgacca
    31441 atgctataga caatcccaaa tacaagcaag tagccgatgc ctgcataaaa gaccttatcc
    31501 agctgaaaga gttgatccga tgggaaaaag aaagtcccct gaccttcctg cgaatttgca
    31561 taagggagca ataaatggaa ttgctctcca agccccttat aaaactggcc agccataaaa
    31621 gccgatgcca tggctgaaat caggtaataa acctgtaaga gcaggcctct ccgatagccg
    31681 atataaaatc cccaaaccaa gaccaataga agaaggaatg aaatcataag gaatcctcaa
    31741 tcttgctctg ttcttgctta caagtcacaa gcttgtgacg gagttcttct agctcttgct
    31801 ccttatcgtc aaattcaatc tcacggctga gctgagttga taaacagttg actgccaaca
    31861 aaagagcgat tgtttcatca tctgcgctag gcatttgttc tttaattgct tggtattttt
    31921 ctgtcgcaac cttagcgatt tcctccataa aaaggttatc atgttcgctt gtcaaggtta
    31981 acgatttttt cccgaatgta aatttgaatc gatttagatt tgccataaaa ttcacctcac
    32041 gatattatac caaaattcgc taattttgtc agtttttaca aattttactg cttttgtggt
    32101 acaatagaaa ctatggcaag tatcacactc acaccaagcg aaaaggatat tcaggctttt
    32161 cttgaacact atcaaaccag tctggctcct agcaagaatc cctatatccg ctactttttg
    32221 aaactacctc aagcaacggt ttctatctat acttctggaa aaatcttgct tcagggtgaa
    32281 ggggctgaaa aatacgccag tttctttggc tatcaagctg tagagcaaac cagcggacaa
    32341 aatcttcctt taattgggac agatgaggtg ggaaatggtt cctactttgg tgggcttgca
    32401 gttgtggctg cctttgtcac acctgaccag cacgactttt tacgaaaact cggtgtgggg
    32461 gattctaaga ctctgaccga ccaaaagatc cgtcagattg ctcctattct caaggaaaaa
    32521 atccagcacc aggcactcct tctctcaccc agcaagtaca acgaggtcat cggagaccgc
    32581 tacaatgctg tttcggttaa ggttgccctc cataatcagg ctatctatct cctccttcaa
    32641 aaaggtgttc agcctgagaa aattgtgatt gatgccttta ccagtgctaa aaattatgac
    32701 aagtacttgg cacaagaggc caatcgtttc agcaatccta tcagcttaga aaaaaaggct
    32761 gagggcaaat acttggctgt cgcagtttct tctgtcattg cgcgtgatct ctttctggaa
    32821 aatcttgaaa atctgggacg agaactgggt tatcagctcc caagtggagc tggaacagct
    32881 tctgacaagg tggctagcca gattttgcaa gcctatggta tgcagggact cagcttctgc
    32941 gccaaattgc actttaaaaa cactgaaaaa gcgaaaaaac gcttagaaag gtaagttatg
    33001 aattcattta aaaatttctt aaaagagtgg ggattgttcc tcctaattct gtcattacta
    33061 gctttaagtc gtatcttttt ttggagcaat gttcgcgtag aaggacattc catggatccg
    33121 accctagcgg atggcgaaat tctcttcgtt gtaaaacacc ttcctattga ccgttttgat
    33181 atcgtggtgg cccatgagga agatggcaat aaggacatcg tcaagcgcgt gattggaatg
    33241 cctggcgaca ccattcgtta cgaaaatgat aaactctaca tcaatgacaa agaaacggac
    33301 gagccttatc tagcagacta tatcaaacgc ttcaaggatg acaaactcca aagcacctac
    33361 tcaggcaagg gctttgaagg aaataaagga actttcttta gaagtatcgc tcaaaaagcc
    33421 caagccttca cagttgatgt caactacaac accaacttta gctttactgt tccagaagga
    33481 gaataccttc tcctcggaga tgaccgcttg gtttcgagcg acagccgcca cgtaggtacc
    33541 ttcaaagcaa aagatatcac aggggaagct aaattccgct tctggccaat cacccgtatc
    33601 ggaacatttt aagagaccta agaggccgag aatcaccaat ctcagcctct tcttctatcg
    33661 tgagaagtga ttgtttattg acaaatactc aatgaaaatc aaagaacaaa ctaggaagct
    33721 agccgtaggc tgcttaaaac agtgttttga ggttgtggat agaactgacg aagtcagctc
    33781 aaaatactgt tttgaggttg cagatggaag ctgacgtggt ttgaagatat tttcgaagag
    33841 tataaaataa aaacaatccc aactctcacc tattcatgca aaggaatttt atggaagttt
    33901 atttttcagg aaccattgaa cggattattt ttgaaaatcc cagcaatttt tatcgcatcc
    33961 tcctcctaga aatcgacgat acggacgcag aggattttga tgattttgaa atcattgtca
    34021 caggaaccat ggctgatgta attgagggcg aagactatac tttttggggg caaattgtcc
    34081 agcactccaa gtatggagaa caactgcaaa tcagtcgtta tgatcgcgca aaaccaacta
    34141 gtaagggctt ggtcaagtac ttttcaagta gccatttcaa ggggattggt ctcaagacag
    34201 ctcagaaaat cgtggatacc tatggcgaaa ataccattga cgaaattttg caacacccag
    34261 aaaagttaga aggcatcgca ggactctctg ccaaaaatcg cgaggctttc gtctccactc
    34321 tccgtctcaa ctacggaacg gagatggttt tgtccaaact agccaactac ggcattccca
    34381 acaaactagc ctttcagatt caagactttt acaaggaaga aacccttgat gtggttgaaa
    34441 attatcccta ccagctggtt gaggatatca agggtttggg ctttaccatt gctgaccaac
    34501 tagctgagga actaggcatc gaaagtcagg ctcctgaacg cttccgcgcc ggtctagttc
    34561 acagtctttt tcaggcctgt atggaaacag gggacaccta tgttgaagca cgggatttgc
    34621 tggaacaaac ccttactctc cttgagtctt cccgtcccgt ggaactagac cccagccaag
    34681 ttgcccaaga gctctcctac ctgatcgaag aagacaaggt tcagcagatt gataccaaga
    34741 tctttgacaa cagcctcttt ttcgctgagg aaggcatccg cagtcacttg gttcgtatcc
    34801 ttgaaaaagg aaaacagaag agtcatgatt tagaaaccat tcaaaaacat atcactacta
    34861 tcgaggaaga gctgggaatt gagtatgata acattcaaaa acaggctatc tgtgatgcta
    34921 tccagaataa ggtctttatc ctgacaggtg ggcctggtac tggtaagaca actgttatca
    34981 atggaatcat cgctgtttat gccctgttag agggacttga cttcaggaag aaaagcaatc
    35041 tgcctattct tcttgctgct ccaactggtc gagctgctcg tcgcatgaat gaattgacag
    35101 gtttgcctag cgcgaccata catcgccact tgggaatgac aggtgacgat gataccagtc
    35161 atctggaaga ttacctagat gctgacttta tcatcgtgga tgaattctct atggtagata
    35221 cttggctggc caatcaactc ttctccaata tctcttctaa cagtaagatc ctcatcgtag
    35281 gtgacagtga tcagctacct tctgtcagtc ctggacaggt tctagcggat ctgcttcata
    35341 ttcctttgat tcctcagact cacttggaaa aaatttatcg acaaagcaaa gaatcaacca
    35401 tcgtcaccct agctagtcag attcgacagg gcatcttgcc agctgatttc acccaaaaaa
    35461 aagctgaccg ttcctacttt gaaattgcta gtggccatat tcctgccacc attgaaaaaa
    35521 tcttaggtgc cgccctcaga aatggtattc ctgcccgtga tatccaagtt ctggctccta
    35581 tgtaccgagg gacggcaggg attgatgcta tcaatcagct catgcaagac ctgctcaatc
    35641 ccccacaaaa agatcaactc agttttgaag ctcctcagtg ccactatcgt aagggagaca
    35701 aggtcattca tttggttaac gatgctgaaa tcaatgtctt taatggggat ttaggagcta
    35761 tcacagacct gattcctggt aaatacaccg aatcgaaaca agacgagatt gtcattgatt
    35821 ttgatggtaa tgaagtctct tacccccgta acgaatggta caagattcgc ttggcctatg
    35881 ccatgagtat tcataagtct cagggaagtg agttcccagt tgtcatccta ccgattacta
    35941 gtgctagccg acgtatgctg gagcgaaatc tcatctacac agccattaca cgtgccaaaa
    36001 gcaaactcat cttactaggc gaattacaag ccttcgacta tgctacccaa cacatcggaa
    36061 ctgcccgaaa aacctatctg attgaacgct tcagtgatct actggagaat gttgaagaaa
    36121 agcaacaagc tgtctctgaa acagtcacat caagtgcctc tgaacaatcc tacatcctaa
    36181 ccgaagaaaa ctgggacagc attccagcca tgattgggat tacagacaca gacctcaaag
    36241 agatttttgg aaaatagtgc atcagaaaaa ccaccaattc aggtgggttt tctgtttatt
    36301 aagattattt tactgttgct gtgcttgtaa tcaattcaac agcttttttg atagtgatat
    36361 catgtttcaa catgtcagct gaaagcaagt tttgaacttg tgcaacttcc atgttgtagt
    36421 ctgctgccaa ttgctcaact tctttttgga tttcttcttc tgaagcatca aatccttcag
    36481 ctttggcaac tgcttcgata acaaggttag tcttagtacg tgactcagct tctgcttggt
    36541 attggttgtg aaggtcttct tgagtagttc cagtaatttg gaagtacatg tcagggttga
    36601 tcccttgacg ttgcaagttc ccaaggaatt catttactga acggtgaact tcttcgtgga
    36661 tcatttcttc tggaagttct acgatttcag cattttctac agctgtatca attgctgcac
    36721 cttcaactgc atctttgtaa gcttcttctt tagcagcagc caattctttg cggtattttt
    36781 ctttcaagtc agcaagtgtt tcaacttctt catcaatgtc ttttgcaagt tcatcgtcaa
    36841 gagctggaac ttctttagct tttacttcgt ggatagttgt cacgaattta gcttctttac
    36901 ctgcaaggtc ttctgcttgg tagtcttctg ggaatgttac gataacatca acggtttcgc
    36961 cagctgagtg acctaccaat tggtcttcga aaccagggat gaattgacct gaaccaagtc
    37021 caagtgagaa gttttcacct tttccaccgt caaattcaac accgtcgata gaaccaacga
    37081 agtcgatcac aacagtgtcg ccgtcttcag cagcagcttc cttgataacc aattcagcca
    37141 ggttgttgcg ttcacgttcg atacgctctt cgacatcagc gtcagttact tctttttcta
    37201 catcaactga tacttcaagg tttttgtagt cacccaattt tacttcaggt tttgtaacaa
    37261 cttcagcagc gataacccag tcttgacctt tttccattga agttacgtca atttttggtt
    37321 gggcaaccac ttcaagacca gcctctttta cagctgcttc ataagcgttt ggcaaaagag
    37381 cgttcataac gtcttggtaa agtgattctt caccaaattt tttatcgaag ataggacgtg
    37441 gaaggtgacc tttacggaaa cctggaacat taagagattt cttcactgac ttgaagacac
    37501 ggtccaattc tggtttgatt tggtcttgag agatagtgaa agtcaagaca ccacggtttg
    37561 tttctttgtt ttcaaatgat acagacattc tgtcatttct ccttaaaatt ttttaaatac
    37621 agtctattat aacattttct ataaataaaa tctagactaa tgcagacaat tatagacaac
    37681 aagccttttt cctaactaaa acactcataa agtatagaca attttagact actttggata
    37741 tcttttggtt agtcattttc caaaactaat ttatctaatt tttaagttat atttgacttc
    37801 atcttctcag taacatgagt ataaacactt aaaatctttg tttcattagt ataatctaca
    37861 cgatccatta tcgctgttat aggtacttcc aattcagcaa gcaaacttat atgcgaatat
    37921 ctaaaaacat gagcagtcaa tacaaaatca tctttcttaa tttcactttt cctaaacctg
    37981 tttttcaaaa agtaattgaa acaagttatc attaagggag aaccattatt tgtaaatata
    38041 taatcacctt cttctaaaag aagatattct tttaaaattt ctaacactct atcactaata
    38101 ccaattactc tatatgaagc taaacttttc ggactatcca aaaacacttc gttattggac
    38161 gattacatta tagtaaaacg aaataagaac aggacaaatc gatcaggaca gtcaaatcaa
    38221 tttctaacaa tgttttagaa gtagaggtgt actattatag tttcaatcta ctatacaaga
    38281 cgaaattgaa gaaatctttc cattataaaa cgcatattat caaggttttt aaacacctaa
    38341 taatatgcgt tttttcctat tttaaagact tttatccacc tattatacaa agttaagtat
    38401 ccatcacagt ttacaattag ccttcattac ctaacccatt caccatcctt caagagagta
    38461 acctttaaga accgaataaa taaatggcaa atttcgttca tttaatttaa ctgcactgtt
    38521 aatttgatta gctccatctc ctaaagtatg cactcctact acgcggttac tagcatcata
    38581 aactgtagat ccactagacc cctctaaagt atcaacttgg tagtccaaga acatgccatc
    38641 atcacttaaa acttgttttt tatctgtata catttgatga attttaaaat tatatgatgg
    38701 atagcctgtg atagtcacag ttattcctgt caaatttttt tgactagtag gaagacccaa
    38761 agtccctaat tttgcaccaa tgggctcttc tagaattaat aaagccaagt catattccct
    38821 tgcatcctta gaatttaaat ttctaaattc cttcaaataa cgaacttctt ttacttttat
    38881 ccgaccaaac ggcacactat ttggagtacg agcaggagta acatatagtt cttctgcatc
    38941 tttaccaaac tcatgtttat aaatattatg agcagcagta ataaggacat tatcctttat
    39001 aaaattggca ctccctcgat aagttacatc tttaccattt ttcacaccac gaaattctga
    39061 ctctacataa gctgatgtag aataaggata ttgtaaagta ttttttacct taactaaatc
    39121 atttttacca ataatgactt ctgactgatt tgtataatat tgaccataag tcggtgaaac
    39181 attgggaagt gaaaaat
//
LOCUS       DAVKJJ010000014        38544 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000014 DAVKJJ010000000
VERSION     DAVKJJ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38544
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.014"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(693..1265)
                     /locus_tag="VW144_000721"
     CDS             complement(693..1265)
                     /locus_tag="VW144_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600203.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGIYLAQLWDKNDAIPNNGRINF"
     gene            complement(1886..2734)
                     /locus_tag="VW144_000722"
     CDS             complement(1886..2734)
                     /locus_tag="VW144_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9600204.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYNKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            2886..3164
                     /locus_tag="VW144_000723"
     CDS             2886..3164
                     /locus_tag="VW144_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600205.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            3166..5229
                     /locus_tag="VW144_000724"
     CDS             3166..5229
                     /locus_tag="VW144_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9600206.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(5307..6563)
                     /locus_tag="VW144_000725"
     CDS             complement(5307..6563)
                     /locus_tag="VW144_000725"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU9600207.1"
                     /translation="MHIFDELKDRGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADEHNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            6705..9170
                     /locus_tag="VW144_000726"
     CDS             6705..9170
                     /locus_tag="VW144_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9600208.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMTKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALVPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            9235..10146
                     /locus_tag="VW144_000727"
     CDS             9235..10146
                     /locus_tag="VW144_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9600209.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVSVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            10376..11074
                     /gene="dapD"
                     /locus_tag="VW144_000728"
     CDS             10376..11074
                     /gene="dapD"
                     /locus_tag="VW144_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEU9600210.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            11142..12272
                     /locus_tag="VW144_000729"
     CDS             11142..12272
                     /locus_tag="VW144_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEU9600211.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEAGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            12284..12823
                     /locus_tag="VW144_000730"
     CDS             12284..12823
                     /locus_tag="VW144_000730"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEU9600212.1"
                     /translation="MKSELRKQVLHEMKALSQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMDFVVYDLQQLVKTAFALLEPQ
                     GDLEVVDVSQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            12807..13484
                     /locus_tag="VW144_000731"
     CDS             12807..13484
                     /locus_tag="VW144_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU9600213.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIILRYATRNPYIQQLGQSYMTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRLYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(13551..14450)
                     /gene="galU"
                     /locus_tag="VW144_000732"
     CDS             complement(13551..14450)
                     /gene="galU"
                     /locus_tag="VW144_000732"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEU9600214.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDEATGMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMNDYEKTHAST
                     IAVMPVPHEDVSSYGVIAPQDEGKDGLYSVETFVEKPAPEETPSDLAIIGRYLLTPEI
                     FEILEKQAPGAGNEIQLTDAIDTLNKTQRVFARKFTGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(14472..15488)
                     /locus_tag="VW144_000733"
     CDS             complement(14472..15488)
                     /locus_tag="VW144_000733"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9600215.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKPYFKDVVLDENIIAYTDLAETLKNVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVAVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            15700..16080
                     /locus_tag="VW144_000734"
     CDS             15700..16080
                     /locus_tag="VW144_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9600216.1"
                     /translation="MRKFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLGVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIAQKKESVLIR
                     FLKSIMIGYLRKKDLSSQIIHIWH"
     gene            complement(16254..16679)
                     /locus_tag="VW144_000735"
     CDS             complement(16254..16679)
                     /locus_tag="VW144_000735"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9600217.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKSLTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGETCLTE"
     gene            complement(16747..17397)
                     /gene="phoU"
                     /locus_tag="VW144_000736"
     CDS             complement(16747..17397)
                     /gene="phoU"
                     /locus_tag="VW144_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9600218.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(17412..18164)
                     /gene="pstB"
                     /locus_tag="VW144_000737"
     CDS             complement(17412..18164)
                     /gene="pstB"
                     /locus_tag="VW144_000737"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9600219.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(18166..18981)
                     /gene="pstA"
                     /locus_tag="VW144_000738"
     CDS             complement(18166..18981)
                     /gene="pstA"
                     /locus_tag="VW144_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9600220.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(18974..19837)
                     /gene="pstC"
                     /locus_tag="VW144_000739"
     CDS             complement(18974..19837)
                     /gene="pstC"
                     /locus_tag="VW144_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9600221.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(19955..20830)
                     /locus_tag="VW144_000740"
     CDS             complement(19955..20830)
                     /locus_tag="VW144_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEU9600222.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(20997..22328)
                     /locus_tag="VW144_000741"
     CDS             complement(20997..22328)
                     /locus_tag="VW144_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEU9600223.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSALKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(22329..23036)
                     /locus_tag="VW144_000742"
     CDS             complement(22329..23036)
                     /locus_tag="VW144_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9600224.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(23120..23422)
                     /locus_tag="VW144_000743"
     CDS             complement(23120..23422)
                     /locus_tag="VW144_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU9600225.1"
                     /translation="MTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQLHYC
                     VPKGTIWGSTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTRY"
     gene            complement(23409..23630)
                     /locus_tag="VW144_000744"
     CDS             complement(23409..23630)
                     /locus_tag="VW144_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU9600226.1"
                     /translation="MNKSYFNPLAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINL
                     AGKGRALYTSIYFLLRRNESFTFSPIDSR"
     gene            23592..24148
                     /locus_tag="VW144_000745"
                     /pseudo
     CDS             23592..24148
                     /locus_tag="VW144_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(24463..26154)
                     /locus_tag="VW144_000746"
     CDS             complement(24463..26154)
                     /locus_tag="VW144_000746"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU9600227.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPEIAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            26290..26736
                     /gene="argR"
                     /locus_tag="VW144_000747"
     CDS             26290..26736
                     /gene="argR"
                     /locus_tag="VW144_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9600228.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            26787..29321
                     /gene="mutS"
                     /locus_tag="VW144_000748"
     CDS             26787..29321
                     /gene="mutS"
                     /locus_tag="VW144_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU9600229.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            29468..31162
                     /gene="patA"
                     /locus_tag="VW144_000749"
     CDS             29468..31162
                     /gene="patA"
                     /locus_tag="VW144_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEU9600230.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEVIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVLMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQDGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(31493..31762)
                     /locus_tag="VW144_000750"
                     /pseudo
     CDS             complement(31493..31762)
                     /locus_tag="VW144_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            31954..33720
                     /gene="patB"
                     /locus_tag="VW144_000751"
     CDS             31954..33720
                     /gene="patB"
                     /locus_tag="VW144_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEU9600231.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            33859..34548
                     /locus_tag="VW144_000752"
     CDS             33859..34548
                     /locus_tag="VW144_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9600232.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            34552..34731
                     /locus_tag="VW144_000753"
     CDS             34552..34731
                     /locus_tag="VW144_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600233.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            34718..36067
                     /locus_tag="VW144_000754"
     CDS             34718..36067
                     /locus_tag="VW144_000754"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU9600234.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            36191..37651
                     /locus_tag="VW144_000755"
     CDS             36191..37651
                     /locus_tag="VW144_000755"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU9600235.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREEFIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            37665..38099
                     /locus_tag="VW144_000756"
     CDS             37665..38099
                     /locus_tag="VW144_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9600236.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNEQ"
     gene            38223..38294
                     /locus_tag="VW144_000757"
     tRNA            38223..38294
                     /locus_tag="VW144_000757"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38256..38258,aa:Glu,seq:ttc)
BASE COUNT        11302 a         7323 c         8367 g        11552 t
ORIGIN      
        1 tttttatgat atacacactt aaattggtgt tgtttattac ctttcttgta ataagcttgt
       61 tacctgataa gatttttgga aaaaataaaa aaatttggaa aatagttttt gcaatattga
      121 cggcagtggc agcattgtca tttatgtact aagttatttt aagaatgtag ggaaataaac
      181 cctacatttt ttttagtttt ttctgttttc taaattctat ttatccaaga gcttcaacat
      241 ttcttgcttc ttcgcttcaa gttctgcacg cttttcttcg atttcggcat gttttttctc
      301 gagttcagaa caacttgcat cattgctaaa ttcttttcgc catcaggaga tagggtgagt
      361 ctacatgtct attactcacc caaagcagtc ctacaaagca ggaattttct gttacttttt
      421 tggaaatagt aacgtttata cagctttgac acttcgtatc aaagcgccaa acacactccg
      481 agggatttac agaaagcaga aaaggaatga tctggtataa gatcattcct tttctctctt
      541 tttctttaag taattatata caatgtacga cgaagtcgtc attgcaatgc tgatccacca
      601 cctaaaggga actttaaaca acattgataa gataaagaat ataaacaacg aaaatacgtt
      661 atacccaatt aattttattg tatatctcat gattaaaagt taatccttcc gttgttagga
      721 atggcatcat ttttatccca taattgtgct aaataaatcc ccggtgataa taaattcata
      781 gcgaattcta aagcaacatc atttacaaac caactaccta gatatctaga aattgctgaa
      841 cgaatagcac tttttgctgc atgttttcct tttactttaa ttagatttgc aaggcctgca
      901 gtagttcctc ctaatgctaa agctattgca gtatctaata gagcacccat ttgattaact
      961 gtaatacctt gccaagctgc tctaaatgga gagtatgtag gtgggattgt ataatcgcct
     1021 tgtaattgtc ggttaattac ttctttgatc cattgttgtg agacgtctgg atgaaaagat
     1081 tggatttcgt ttgcaagtgt attgatttgt tcttctgtta gagaagtgac aggttgaagt
     1141 tccatatttg tttcaatttg tgatacttgt tcagaagcgt atacagctga aacacttgga
     1201 atcgctgata caattaacac aattgacgtc aaaaaaaccg aaataaattt cattaatttg
     1261 ttcatgagct tttctccttt ttatttgtat ctgcttacat tttatcatat actgttatta
     1321 tagtcaaaaa aatatgctat tatgttaaaa aatatttttc aaaatataaa tggacggatt
     1381 tattttggat tttatttgtt attttgacct gcctctatat tggtaaccat gatttgttta
     1441 ctctcaatca tcaagaattc tcttttcgtg gtagcgtttg gggtctggta ctggccttat
     1501 atcacttatt gttcattgat aagtttgtaa tttcgaatcg aaaataaaga ttagaactat
     1561 gattgactgg ctacttttaa tatttatttt ggaggaagat tttgtctcta ttatttatta
     1621 ttttaaattt atctattttg tacaagattt attctttgag acgggagaaa tcgaaatact
     1681 tgatttatac ggcctatatc atatttgggg taaatgtagt atatggtatt caatggttat
     1741 taagagaact gatttcaact atttcccctt aatgtgtatg gaaactataa caaaacgcat
     1801 aagattaagg tttgtgtacc tgttcttatg cgtttttata atattgaaga ttttcggtaa
     1861 gttttatacc tttgggaagt ctagtttaga atgctttccc aaccaaaatc tctgcttcga
     1921 tggtaatctc tgtcagttgg ctccagtcaa tcttgctctg gtcaacgtga aagaggagag
     1981 gagtcatact gagtagggct tggagttgtt ctgctgtgat agtcttagtc agagaggcag
     2041 tttgactaga taggatggta aagtgttcct ggaaatgctc cttgatatct tggttagaat
     2101 actccttgtt tgtcagctgg tcctgtactc tttgacggat ttctttgagg tgattttcag
     2161 ttgggataac ctttatcaag ataccgtctt tggataaaac gcgacgaaat tctccatagt
     2221 tggcaggtga gaagatatca agcagaatat ccatgttagc gtcttttata ggaagtcgtg
     2281 ccaagtcgcc aacaaaccaa ttgactgccc agttgggttc acttttagca gcgatttgga
     2341 ctgaatcttt ggagatgtca aaggcataga aagttttttc agagtgactt tcttgtagtt
     2401 tgcgagaata gaatccttca ccacaaccga tatccaaaat tgttgtggta gtttttgagc
     2461 ttgcaagcaa gtcagataca gcatctaaga tagcttggta aaagccggct tctaggattt
     2521 gttgacggtt ttgaaaattt tccttgttat agttagcaga ttgcttgatt tgaggtgcta
     2581 gattgacata gccaaatttc gccaagtcaa aagaatgacg gttgcagcac ttgaaattag
     2641 tctctaacag agtcagattt tcttgacaga taggacaggc aaaggcagtc gcagaagcaa
     2701 aacgctgaag tttgggcttg agatttgtat tcatagttta agtgtatcaa aagaggcaaa
     2761 tgaaagcaac tttaggaata agtagatggg agattcagta gaaataaaac taattctcca
     2821 atttatagaa tgaaattgct tttatatcta aattgctata taataatgat aaggaggaaa
     2881 taagtatgtt aaaagaagta ttaaccgttg caaaagttgc gaaaaaatct tcattatttt
     2941 tgggtggtgt cgcatttggt acccttggtt tgaaaatctt agcaagtaag gaagctaaaa
     3001 aaggttattc taaagctttg gctaaggctt acaagttgaa agacgagcta gatgcatctg
     3061 tttctgttgt gaagcaacat ggagacgatg tcttgcaaga tgccaaatat ttgtacgagc
     3121 aagagaaaaa agaagagcaa ttagatagcc ttataggtga ataatatgtc ttttaaagtg
     3181 ctacatagag gataccaaca tatccgacta tcatcttctt tttcacttac cttggatatt
     3241 caagactatc ttcgttcctt ggcgagagat gaaaagggga ttgagtctat ccagttttac
     3301 atggatcaac agcactttac tctacgcata aaagaaggct tttctgtatt agataatgca
     3361 gaagcctttt taaaaagaat tgataaaggg aaagtttctg agttgatgac tcttcccatt
     3421 cgtagagaag agagtgctta ttctattgtt tcaggtgcag cggttaagcg tgtacttttt
     3481 cgtagttttg tgccgtatcc tattcgctat atatggactt gttatcaggc tttgggttat
     3541 attagagaag cctatcaaac actagcgcgt aaggaactaa ctatggaagt cttggactgt
     3601 tcggctattt tattgtcttt gtttatgaac caatccaaga cagctagcaa tatcatgttt
     3661 atgcttgatt tggggaatca tttagatcag tggtccttga aaaaaactgc aacagattta
     3721 gaacagagtc ttcttgcaaa agagagcgat gtattcctag tacagggcga tacggttgtt
     3781 agtataaaga gttccgatgt tcaaatagga gatgtcttga tcttatctca aggaaatgaa
     3841 attctgtttg atgggcaagt agtttcaggt ttaggtatgg tcaacgaaag ttccttgaca
     3901 ggagagagtt ttccagttga aaaaagagag tctgatttgg tttgtgcaaa tacagtatta
     3961 gaaactggag agttacgcat tcgtgtaaca gataatcaga tgaacagccg tattttacag
     4021 ctgattgagt tgatgaagaa atctgaagaa aacaagaaaa cgaaacaacg ctatttcatc
     4081 aagatggcgg ataaagtcgt caaatataat ttcttggggg ctgggctgac ttacctattg
     4141 acaggttctt tttctaaggc tatttctttc ctattggtcg atttctcctg cgctttgaaa
     4201 atctctactc ctgtagctta tttgacagtt atcaaggaag ggttgaaccg tgaaatggtg
     4261 attaaggatg gagatgttct ggagaaatat ctggaagttg atactttctt gtttgataag
     4321 acaggaacaa tcacaactag ttatcctata gttgaaaagg tgttaccttt tggagactat
     4381 agtgaggaag atattctcag aatcagtgcc tgtcttgagg aacacattta tcatcctatt
     4441 gctaatgcca tcgtcaagca agctgagata gagggaattg aacatgagga aatgcatggg
     4501 aaactccaat atatcgcaag caaggggatc aaatctcata tagatggcca accagttctt
     4561 attgggaatt atgtcttgat gcaggatgag cagattcata tcagttcaga acaaaatgct
     4621 ttaattgaag agtacaagag tcactacaat ctcttattct tggcttatca aaatgaattg
     4681 attggaatgt tctgcattca tactcctttg agaaaagaag caaaaacagc cttggataaa
     4741 cttaaggcac aggggaaaaa attgattctg gcaacaggag acaccttgat taggacagag
     4801 gaattagtca aagatttgcc ctttgatcag gtctatacag acttgaaacc tgatgggaaa
     4861 tttgagttag tagagaaact gcagaaagca ggtcacacta ttttgatggt tggagatgga
     4921 ttgaatgact cagcggcttt aactctatca gatatcggtg tggtgatgaa tgagagtgca
     4981 gatatttcta agcagatgag tgatatctta ttgttagata atcgtttgga tttcttccaa
     5041 gagttggatt cgttatcatc atctttgcaa acactcatca agaaaaatat tcaagatacc
     5101 gttgtcgtaa atagtagttt gattggcttc ggcttattta attggctcag tccttcaaat
     5161 ctctctatct tacataatct aacaaccttg cgcattgtcc tgcgtagcct gtctattaaa
     5221 aatagataga tgagagttat taacagcaaa aaggagttac ctttctcgag gttaactcct
     5281 ttgtttatag ataaatgttg aatagtttag taagtcaata caaagtattt tttcttccca
     5341 cgacggataa cagtcagttc attttctaac ttatcagcgt cactcaagac atagtcaagg
     5401 tcttggatgc ggtcgccgtt tacgtagatg gctccgtttt ggacgtcttc acgggcttgg
     5461 cgttttgagt taactatacc agatgagacg agcagttcca cgatattgtt gtgttcgtct
     5521 gcctgtactt ggtagtttgg cacaccacga agtccttgtt tgagctcttt gacagaaagg
     5581 tttttgatgt ttcctgcaaa gagttgctca gtgatgttaa gtgcttcttt gtaggcttct
     5641 tctccgtgaa caagtgtaac aacttcacga gccaagactt tttgagccaa gcgttcgtgt
     5701 ggcgctgctt caaattgttt acgaatatct tcaatctcat caagtgacaa gaaagtaaag
     5761 attttcaaga agcgaacagc gtcagcgtcc atcacgttca tccagaattg gtacatttcg
     5821 tatggagaag tcttttcggg attgagccag acggcatttc cttctgattt accaaatttc
     5881 ttaccagttg catctgtgat tagtggaaca gtgataacgt gaccagtctt gtccgcctta
     5941 cgacgaagca attcagtacc agctgtcata tttccccact ggtcagaacc accgatttga
     6001 agagtgacat tatggtcttg gttaaggacg aagaagtcgt acccttgcat gatttggtaa
     6061 gcgaactcag tgtaagaaat tcctgtttcg atccgttttt taacagattc cttactcatc
     6121 atgtagttga ccgtgaagta ttttccaata tcacggagga agtcaatgaa gctgatgctg
     6181 ccaaaccagt cgtagttgtt gaccatgaca gccttgtttt cgccattttc aaagtcaaga
     6241 aaacgagaaa gttgtccttg gatagacttg acccagccat ctactgtgtc ttttgtttgg
     6301 agactacgtt cagcatcttt gaaggacgga tctccgatga gacctgtagc accgccaacg
     6361 agcgcataag gtttgtgacc tgctagttgc aagcgacgac ttgtcaagat tgcgacaagg
     6421 tggcctaggt gaaggctgtc agcagttgga tcgtagccag tataataaga aacttgacct
     6481 tcttctaggg ctttacgcaa agcttcttca tcagtcgttt gaaatatcaa accacgatct
     6541 tttagctcat caaaaatgtg catgtgtctt ttctcctttt taaaattatt gtttctacct
     6601 attgtatcac aaacttgggc aatagcctag cagaatagga aagaaagtgg ctattttatt
     6661 gaaaatttcc cttttgtggt ataatagata aagtgaggaa atccatgcaa aatcaattaa
     6721 atgaattaaa acgaaaaatg ctggaatttt tccagcaaaa acaaaaaaat aaaaaatcag
     6781 ctagacctgg caagaaaggt tcaagtacca aaaaatctaa aaccttagat aagtcagcca
     6841 ttttcccagc tattttactg agtataaaag ccttatttaa cttactcttt gtactcggtt
     6901 ttctaggagg aatgttggga gctgggattg ctttgggata cggagtggcc ttatttgaca
     6961 aggttcgggt gcctcagaca gaagaattgg tgaatcaggt caaggacatc tcttctattt
     7021 cagagattac ctattcggac gggacggtga ttgcttccat agagagtgat ttgttgcgca
     7081 cttctatctc atctgagcaa atttcggaaa atctgaagaa ggctatcatt gcgacagaag
     7141 atgaacactt taaagaacat aagggtgtag tacccaaggc ggtgattcgt gcgaccttgg
     7201 ggaaatttgt aggtttgggt tcttctagtg ggggttcaac cttgacccag caactaatta
     7261 aacagcaggt ggttggggat gcgccgacct tggctcgtaa ggcggcagag attgtggatg
     7321 ctcttgcctt ggaacgcgcc atgactaaag atgagatttt aacgacctat ctcaatgtgg
     7381 ctccctttgg ccgaaataat aagggacaga atattgcagg ggctcggcaa gcagctgagg
     7441 gaattttcgg tgtagatgcc agtcagttga ctgttcctca agcagcattt ttagcaggac
     7501 ttccacagag tcccattact tactctcctt atgaaaatac tggggagttg aagagtgatg
     7561 aagacctaga aattggctta agacgggcta aggcagttct ttacagtatg tatcgtacag
     7621 gtgcattaag caaagacgag tattctcagt acaaggatta tgaccttaaa caggactttt
     7681 taccatcggg cactgttaca ggaatttcac gagactattt atactttaca actttggcag
     7741 aagctcaaga acgtatgtat gactatctag ctcagagaga caatgtctcc gctaaggagt
     7801 tgaaaaatga ggcaactcag aagttttatc gagatttggc agccaaggaa attgaaaatg
     7861 gtggttataa gattactact accatagatc agaaaattca ttctgccatg caaagtgcgg
     7921 ttgctgatta tggctatctt ttagacgatg gaacaggtcg tgtagaagta gggaatgtct
     7981 tgatggacaa ccaaacaggt gctattctag gctttgtagg tggtcgtaat tatcaagaaa
     8041 atcaaaataa tcatgccttt gataccaaac gttcgccagc ttctactacc aagcccttgc
     8101 tggcctacgg tattgctatt gatcagggct tgatgggaag tgaaacgatt ctatctaact
     8161 atccaacaaa ctttgctaat ggcaatccga ttatgtatgc taatagcaag ggaacaggaa
     8221 tgatgacctt gggagaagct ctgaactatt catggaatat ccctgcttac tggacctatc
     8281 gtatgctccg tgaaaagggt gttgatgtca agggttatat ggaaaagatg ggttacgaga
     8341 ttcctgagta cggtattgag agcttgccaa tgggtggtgg tattgaagtc acagttgccc
     8401 agcataccaa tggctatcag accttagcta ataatggagt ttatcatcag aagcatgtga
     8461 tttcaaagat tgaagcagca gatggtagag tggtgtatga gtatcaggat aaaccggttc
     8521 aagtctattc aaaagctact gcgacgatta tgcagggatt gctacgagaa gttctatcct
     8581 ctcgtgtgac aacaaccttc aagtctaacc tgacttcttt aaatcctact ctggctaatg
     8641 cagattggat tgggaagact ggtacaacca accaagacga aaatatgtgg ctcatgcttt
     8701 cgacacctag attaacccta ggtggctgga ttgggcatga tgataatcat tcattgtcac
     8761 gtagagcagg ttattctaat aactctaatt acatggctca tctagtaaat gcgattcagc
     8821 aagcttcccc aagcatttgg gggaacgagc gctttgcttt agttcctagt gtagtgaaat
     8881 cggaagtctt gaaatcaaca ggtcaaaaac cagggaaggt ttctgttgaa ggaaaagaag
     8941 tagaggtcac aggttcgact gttaccagct attgggctaa taagtcagga gcgccagcga
     9001 caagttatcg ctttgctatt ggcggaagtg atgcggatta tcagaatgct tggtctagta
     9061 ttgtggggag tctaccaact ccatccagct ccagcagttc aagtagtagt tctagcgata
     9121 gcagtaactc aagtactaca cgaccttctt cttcaagggc gagacgataa ttctctaaat
     9181 gaagtggcca atcaatgaat tgccactttt ttcctttcgt gttacaataa gggtatgaat
     9241 cagtatcaga aaaagattgt taacggaaaa atttattcgc tcctatccgg cttaatatgg
     9301 ggaatctgtg gaattttagg agagtacttc tttactcatt atcaggtgtc ttcgggctgg
     9361 attacctcta tgcgtttgac actggcaggg agtcttgtac tcatttggtc tgcaatacaa
     9421 ttaaaatcgc aagtgctaga tatttggcga gacaagaaaa attacctgcc ctttttagcc
     9481 tatgctattt tggggatttt ttcagttcag tattttttct atctctgtgt agaatactca
     9541 aatgctacga cagcaactat tttacagttt attagccctg tctttatcct cttttacaat
     9601 cgcttggttt atcaaaaacg agcgtcaaaa agcgctgttt tctatgtttt ggttgccatg
     9661 ctgggtgttt gcttgatggc gacaaaggga gacctctctc agttatccat gacgccgcta
     9721 gctcttataa caggtttgct gagtgccatg ggtgttatgt ttaatgttat cttgccccaa
     9781 ccttttgcta agcgttatgg ttttgttcct acggttgggt gggggatgat tttggcaggt
     9841 ttgtttagca atgtcctctc gccggtttat cagctttcct ttactcttga tatttggagt
     9901 atcttgattt gcctcattat cgctttcttt ggaacggctt ttgctttttt catttccatg
     9961 aaggctgtgt ccttggtttc tcctttggtg gtttccgtta tcagtgccag tgaacctctc
    10021 tcttctgctc tcttgagtgt tttgttctta ggattagtag tggattggtc cctccttcta
    10081 gctatagcct tgattatttt acccatgata tttttgtcta tagaagaagc gaaagaaagt
    10141 agataaaaag tcttttctta attctaaaag tgtgctatac tagaatagtc aataaaacac
    10201 ggggagatag ttgtgaagag tgtttttagg ttgtatcggt aggggcttgc ttttaccgac
    10261 agcacgtttt atctcacatc cctaaaaaat catacctaaa aacagacaaa aaggtttcaa
    10321 atttgaggcc ttttttggta gaataggtat cattataacg aaccaggagg cacctatgac
    10381 tgctacaaaa atgaacgctc aagaaattat ccaatttatc gccaatgctg aaaagaaaac
    10441 cagtgtcaaa gtaacctttg aggggcaact cgcaactgct gtgcctagct ctgttgtcaa
    10501 actaggaaat gtcctattcg gagactggaa ggacgtggct ccgcttcttg aaggtttggt
    10561 agaaaatcaa gattatgttg tcgagcaaga tgctcgtaat tctgcagttc ctttgctaga
    10621 taagcgtgct atcaacgctc gtatcgagcc aggtgctatt atccgtgacc aggtggaaat
    10681 tggtgacaat gctgttatca tgatgggagc tgttatcaat atcggtgctg aaatcggtgc
    10741 tggaaccatg attgacatgg gtgccatcct tggtggccgt gccatcgttg gaaaaaatag
    10801 ccacgttggt gcaggtgcag ttttggcagg tgtgattgag ccagctagtg ctgaaccagt
    10861 ccgtgtcgga gacaatgttc ttatcggtgc taatgcagtg gttatcgaag gagtccaaat
    10921 cggtagtggt tcagttgtcg cagcaggagc tattgttacc caagatgtcc cagaaaacgt
    10981 ggtagtagca ggtgttccag ctcgtatcat caaagaaatt gatgcccaaa ctcaacaaaa
    11041 aacagcgcta gaggatgcgc ttcgtacctt gtaattgtaa aagtaaaaaa gaggcggaac
    11101 cctttttcca gcctctttct gctatatcgg aggacagata gatgttagat ttgattcaga
    11161 ctagacgaga tttacaccag ataccagaga ttggcttgga ggagttcaag actcaggctt
    11221 atttgctgga tgtgattgag aaattgacta cgggcaagga ttttgttcaa attcgtactt
    11281 ggcggacagg gattttggtc tacctgcagg gaagtcagcc ggagcgaacc attggttggc
    11341 ggacagatat tgatggcctg cctatcgtcg aacaaacagg tctacctttt gcttctcaac
    11401 accaaggtcg catgcatgcc tgtggccatg attttcatat gaccattgcc ttaggctgtc
    11461 ttgagcgcgc ccttgaggag caacctaaaa ataatctgct ctttctattt cagcctgctg
    11521 aagaaaatga agctggtggt atgctcatgt atgaggctgg tgcttttgga gattggttgc
    11581 caaaccaatt ctatggtctc catgttcgtc cagatttgaa agtcggccag attgcgacta
    11641 atactcatac actctttgca ggaacttgcg aggtgaagat ccgtttcaaa ggcaaaggtg
    11701 gccacgcagc ttttcctcat gaagccaatg acgccttggt ggcggctagt tactttgtga
    11761 cccaagtgca gtcagttgtt agccgcaatg tcaacccaat cgagggagca gtggtgacct
    11821 tcggcgtttt tcaagctgga accaccaaca atgtcatcac agacacagct tttttacatg
    11881 gaactattcg tgccttgaca caggacatga gtctcttggt gcaaaagagg gtaaaaacag
    11941 ttgcagaagg ggttgcagct gcctttgata tggaagtcga agtggaactc aaacaaggtg
    12001 gatacctacc tgttgagaac aatccagcct tggcgcgtga actgatggac ttctttgacg
    12061 agaaagacgg aatcgagttg attgatatcg aacctgctat gacaggcgag gactttggtt
    12121 atctcctttc aaaggttgat ggcgtgatgt tttggctagg tatcgatagt ccctacgctc
    12181 ttcatcaccc tcaaatgagt cctaaggaag aagtcttagc tattggggtg gctgcggtgt
    12241 ctagtttcct gaaaaagaag gcagcagaat agagggcttg tctatgaaat cggaattacg
    12301 caagcaagtc ttgcatgaaa tgaaggcttt atctcaagaa caaaaacagg ctatagacca
    12361 agctttaacc gagcgaattt tacaacaccc cttttatcaa gaagccaagg tcatcgcaac
    12421 ctacctctct ttttctcatg agtttcaaac gcgggaactg attgagcagg cgctgaagga
    12481 cggcaagaag gttttaatac ccaaaactta tcccaagggg cgcatggact ttgtagtcta
    12541 tgatctgcag cagttggtaa aaactgcctt tgccttactg gagccacagg gagatttgga
    12601 agtggtggat gtatctcaga ttgatttgat tcatgttcct ggcctggctt ttacgacgga
    12661 tggatatcgg attggatatg gtggaggtta ttatgaccgc tatctggaac atttttctgg
    12721 tcatactttg agtacggttc atccttgtca aattcaggac tttatacctg aaaaccatga
    12781 tattcctgtt caggaggtat taattgatga aggaaatctt tgatagacgt taccctgtga
    12841 cgagtttctt cctcttagtg acggccttgg tatttttact aatgttggtc actgcaggcg
    12901 gaaactttga cagggcagat acattatttc gatttggagc catgtatggg ccagctattc
    12961 gcctctttcc cgagcaggtt tggcgtctct tgtctgccat ttttgttcat attgggtggg
    13021 aacatttcat tgttaatatg ctttcacttt attatcttgg aaggcaggta gaggagattt
    13081 tcggctctaa gcagttcttt tttctctatc tcttatcagg aatgatgggc aatctctttg
    13141 tttttgtatt tagtcctaaa tccttagcag caggcgcctc tacctctctt tatgggctat
    13201 ttgccgcgat tatcattctt cgctatgcaa cgcgtaatcc ttatatccag cagttgggcc
    13261 aatcctatat gacacttttt gtggttaaca ttattggaag tgttctgatt ccagggatca
    13321 gcctagcagg ccatatcggt ggagcagttg gtggcgcatt tctagcagtt atctttccag
    13381 ttagaggaga aaaacggctg tataacacca gccagagatt aggagcggta gtcttgttcg
    13441 taggactcgc cattttgctt ttctacaagg gaatgggaat gtgacgaagt tgaaagaaaa
    13501 tttgtgtaat gaaaaaagat aaggcatttt ttgaacctta tctttttatt ttattccttc
    13561 tcagtcaatt cttttccaag ttggatgagg taattcttca aatcatcttt gacttgtgga
    13621 tgtttgaggg cgtagtcaat agatgttttc ataaagccaa acttatcccc aacgtcgtaa
    13681 cgagctcctg taaatttacg ggcaaaaaca cgctgtgttt tattaagagt atcgattgca
    13741 tcagtaagtt ggatttcatt tccagcacca ggagcttgct tttcgagaat ctcaaaaatt
    13801 tctggtgtga gtagatagcg accgatgatt gccaaatcac ttggagtctc ctcgggagct
    13861 ggtttttcaa caaaggtttc aacactgtaa agaccatctt ttccttcgtc ttgaggagca
    13921 atgactccat atgatgaaac gtcttcgtgt ggtacgggca tgacagcaat ggttgatgcg
    13981 tgtgtctttt cgtaatcatt catcagttgt tttgtcaaag gaacggcctt ctcgtctgtg
    14041 atgtccatca agtcatcacc cagcataaca acgaagggtt catttcctac aaaagctttg
    14101 gcttgtaaga cagcgtctcc aagaccacga gggtgagttt ggcggatgaa atgaaggcgc
    14161 atgccagttg cttcatctac caattttaac aaatccgttt tgcctttttc cttaagattg
    14221 tattcaagct caaagtttga atcaaagtgg tcctcaatag aacgttttga tttaccagtg
    14281 acaactagaa tatcttcaat acctgatttg agagcctctt ccacgataaa ctggatagtt
    14341 ggtttgtcta cgattggcaa catttctttg gcaagggcct tggttgctgg taaaaatcga
    14401 gttcctagtc cagcagcagg gataactgcc tttctaactt ttgatgtcat aaaaatcctt
    14461 tctatagagg gttaagacca ctcattttct gctttaaatt cattgttcat gatgtcataa
    14521 atggcatctt tgatattggt tccgtgataa ataacttggt aaatagcctg tgtaatgggc
    14581 atatagactc caagttcttg ggctagttca taggctgctc gagtcgttga aattccttcg
    14641 attaccatgc ccatattagc ttctatatca gctagggatt ctcctcgtcc gagagcatct
    14701 ccagctctcc agttacgaga gtggatggaa gttcccgtta cgatcaaatc tcccacacca
    14761 gataagccgc tataggtcaa tggactggcc ccgagtgcta cccctaggcg tgtgatttct
    14821 gctaaacctc gagcgatgat ggctgcctta gcattatcac caaatccaag accatgtaaa
    14881 gctccagcac cgacagcaat aatattttta agagcaccag cagtttcaac cccgataaca
    14941 tccgtattgg tataaagtcg gaagtagtga ttactaaata gctcctgaac gtattgagct
    15001 gtttgtaaat ctttagaagc agcagttatt aaagttaggt cacgcacaat ggtctcttct
    15061 gcatgactag gccctgaaac agcgacgata tcactacgga gatgttcagg aatttcttct
    15121 tcaagaatgg ttgataatcg tttatggcta tcaggttcta atccctttga tgcgtgcatg
    15181 atgataacct tatggtccaa ggtttgtgca acttgctggg caacaagtcg tgtcactttt
    15241 gttgggacaa caaacaaaat cgcatccaca tttttcaatg tttctgctaa gtcggtgtag
    15301 gcaatgatat tttcgtctag aacgacatct ttaaagtagg gcttattagt atggtgtgta
    15361 ttaatttcat tgatttgctc gggaagattt ccccaaatac gtacctcgtg tccattgtca
    15421 tttaagactt gtgaaagggc ggttccccaa gaaccaggcc ccaagacggc gacggtttgt
    15481 ttttccatga tttcctcctt gatagatttc tttctcttat tatatcatat tctaggggat
    15541 tttgcacttg cattactggg gtatggtgga aaaatacaca aagatcgaga tgaagggata
    15601 aactcttagt ctttagttaa agtgagaaga aatctgatag atagtttgat tcagtatatg
    15661 ctataaatag aggtaaacaa ctcaggaggt tcttgtaggt tgcgaaagtt tggcgaaaaa
    15721 attaaaagat tacgtttggc taaaaaaatc agtcgttcag aattttgtgg tgatgagtct
    15781 gaattaagta tccgtcaatt aattagaatt gaaaatggag aatccagacc aacactaaca
    15841 aagttaaaat atatcgctga acgtttgggg gttgaagatt acaagttgat gccaagttat
    15901 atagagttgg ataaggaata cctagaattg aagtatttct tgatgaggac tcctacatac
    15961 gaagatgaaa ctatcgccca aaagaaagag agtgttttga taagattttt gaagagtatt
    16021 atgataggct acctgaggaa gaaagattta tcatcccaaa ttattcatat ctggcactaa
    16081 cgaactacac agttcaaaaa ttaccagaaa agctagttga aatactgtcc ttctggtgat
    16141 tttgcttaat ttctgatgtt cgagacatga ttgaggagat ttgtcttgat tttttcctaa
    16201 aaaagggcag actcctccct tggtttgatg ctcgattttt aaaataaggt gacttattct
    16261 gttaggcatg tttccccctg ccctctagct ctatatggaa cagggagtat cagatttatc
    16321 tggcagattg tatcaaataa ggcttgaaac tgttttgaat aggcgtatag acttgagaat
    16381 ttgagaatat gtcgtctagc agtagagaca tattttccgg caatatgaag gaagagacgt
    16441 cgaaaacgct tgatagtcaa ggactttact tcatcaccag ctagctgctt taaaaagagg
    16501 tagagattat aagccagaca gcccatcatc atacgtactt cattcttaat catggtcgaa
    16561 ctatctgtct tatccccaaa gaatcctgct ttcctttctt tgataaagtt ttcagcattc
    16621 cctcgttcac gatagaattg aaacagctcc tcactatcta cactgcttaa attggtcaaa
    16681 tgaacttttt tacaaccata aaaaagagca cttaatcgta ctcttctctt ttaaggatta
    16741 gttgaattaa ttcaaatcca ctagttctcc tgtttctagg tagactaggc gttcacaaat
    16801 gttagcaatg taatcagcga agcgttctag atgccctatg atataaaggt attgagttcc
    16861 attgggaatt gaggtttctt ggtctttcat aagtccaatg atttcctttg ataaggcata
    16921 ataatattgg tcaatctgtt catctttttg agcaatacta atagcttttg aggcttggtg
    16981 caaaggaaag gcaaccaata aatcggctag catgctgagg gataatttac ccatttggtg
    17041 taactgttct tcgtcagggg ctagttgatt ttcttttagt tgcaaaacag ctttggcaat
    17101 gcctgccata tggtctccca tacgttcaag gtctgaacaa gaagacatga tgctaatcac
    17161 aaatcgaagg tcagacactt gtggctgctg caaggccaac aaacgggcac aggtcaattc
    17221 gatagcgctt tgaccttggt tgatagcatg atccttattg ataattagct ctgccatctc
    17281 cttgtctttg gaggctaagg ccagtaaggc ttttgaagct gtttcaagga caagttgccc
    17341 tagtcctaaa aaggattgtt ctaattcatg caattctaag tcaaattgat ttctcatagg
    17401 tttttccttc cttatccgaa ccgtcctgaa atatagtctt ctgtgcgctg atcttttgga
    17461 ttggtaaaca cgtcaacggt atctccaaat tcgcaaattt ctcctgttaa gaaaaaagca
    17521 gttttatctg aaatacgtga agcttgttgc atgttatggg taacaatgat aatcgtataa
    17581 tcctttttta gttgctgaat gaggtcttca atttttaaag tggagatagg gtctaaggct
    17641 gaagtcggct catccattaa cagaatatca ggttctactg ctaaagctcg cgcaatgcaa
    17701 aggcgttgct gctgaccgcc agataaggac atggcactct ttttaagatc atctttgact
    17761 tcttcccaaa tggctgcccc ttttaaagat ttctccacta aggcatctaa ttgttttttg
    17821 tctcgaatac catgtgtcct tgggccataa gccacgttat catagataga catggcaaag
    17881 ggattaggct gttgaaaaac catccctaca cgcttacgta gctgattaag gttgaatttg
    17941 ctactataaa tatcttgctc atctaagagg acttggcctt caatatggca agaaggaacc
    18001 aaatcgttca tccggttaag ggtttttaga aaagttgatt tgccacaacc agatgggcct
    18061 atcaaggcag taatctgtct ttctggtaat tgaatcgaaa tattttttaa ggcttgaaaa
    18121 tccccgtaaa ataagtctag gtgtctgact gaaaatgttc ccatactagg aagctccttt
    18181 cacaagtttt cgagataata agcttgatag agtatttatc attaaaacag taataatcaa
    18241 aatcacaccg gtagcatagg cttcattgac atgtagcccc tcacttgaca gcatatacat
    18301 atgcagggct agagaacgac ctgaagacat gagactactt ggcgtattgg tagaggtacc
    18361 taatgtatac atgagggcag ctgtctcacc aacgatacgg ccaatagcta gtatcactcc
    18421 agctaaaata cctggcatgg caactggtag aacaattcta aaaacagtcc gtaacttacc
    18481 tgccccaagt ccataacttg cttgacgcat gctatcacta acagataaaa gggcttcttc
    18541 tgttgagcga ataatgactg gcaacaccat gataactgag gttaagattc ctgataacag
    18601 agagtattga aaacctaaga agactacaaa gaagagcatg ccaaacagac caaaaacaat
    18661 ggaaggaatc ccagacaagg tatctgaggc caatcgcatg attttaacac aaagggaatc
    18721 tttttttgta tattccacaa gataaaaacc agcaaaaatc cctatgggca aggctaaaag
    18781 aagagcacca aagaccagaa taacggtgga aataatcgct ggcataaggg aaatgttctc
    18841 agaagtataa gtccaagaaa agagggatag acttagatga ggtaagcctt tgatgaggat
    18901 aaaaccaatg attaaaaaga gagagccaaa ggttaaagct gaaaaacaat aaacgagaag
    18961 ttttagcagg tatttactca taagatgatt ttcctttcaa gtaggcaaag taggcattaa
    19021 tcaagagaat aaggaaaaag agaactgctg aggttgcaat aagggcttcc ctatgctgac
    19081 ctgatgcgta agccatttcc agaacaatat tggttgttaa ggttctggtt cctgaaaaga
    19141 gtccacttgg aataatcggc tggttgcctg ccaccaaaat aactgccatg gtttcaccta
    19201 ctgcgcgacc gattcctaaa ataactgctg ataaaatacc agatctcgca gctggcaaga
    19261 tgacactaaa aatactccgt tcatgactag ctcctagagc caagctacca gaataatacg
    19321 ttttgggaac tgttcggata gcagattctg acaaactgat aatggttggc aaaatcatta
    19381 ttcctaatag taacgaagcg gttaggacac tcatgccatt tcctaaaaag cttctaatcc
    19441 aaggcaccaa taattgtagg ccgaaaaaac cataaacaat agatggaatg gccgccatca
    19501 agttgatagc tgattttaag aagccataga cgggctttgg acaataataa accataaaca
    19561 ccgatgtcaa gatgcctgtt ggcaccccaa tcacaatcgc tcctaaggta attaataagg
    19621 aaccaacgat cattggtaaa ataccatagc ttgccggaat gttcgttggc gaccaatcac
    19681 tgcctaataa aaaacgggca aagccgtagt tagccatgaa aggtaagcca ttactaaaaa
    19741 taaagaaaca gattagcaaa atagctacaa cagctactgt tgcactcatg aaaaaaattg
    19801 ccctaaaaac tgcttctttg aaggcttgtt ttgtcacatc ttgtcctttc tagtgaagaa
    19861 agtaagggag atacgacacc tccctacttg ccttctttat cttattgtac gatgaaacgt
    19921 ctgcatctct ttagagattt atggagcaaa cattttattt aatcttgtcc caggtggtta
    19981 atttgccact aaaaacgtct gcaagttcag ccatactgac ttggcttgcc ttattgtcat
    20041 tattgaccac aacagcaata ccgtctaaag caatagcatc atgggtgaga ctcttacctt
    20101 cttcaggagt taattcccta gaaaccatac caatatcagc ggttttctcc ttaacagcgg
    20161 taatacctgc tgaagaccca ttagaggtaa tatcaatcgt aacttctgga ttttcttttt
    20221 tataagcttc tgctaatttt tccattaaag aagatactga agtggaacct acaacagaca
    20281 acttgcctga taagtgttgg cttgtatatt ctgtggtttc agttttagct tcaataaatt
    20341 tattatctgt gaccacttgt tgaccttgtt tggagtggat aaagctgata aaatcttgac
    20401 ctagcttgga aagattagaa gaccaaacaa tgttgaaggg acgttgaaga gggtattcac
    20461 catctaaaac tgtgtctcga ctagccttga caccatcaat ctctaaagcc ttgacagatt
    20521 tcgttaaaga tcccaaggag atgtagccga tagcattagc attcccttga actgctgaga
    20581 gaacaccttc tgtactattt tgaatcacag ctgttttggc agtgttgtca atttttttat
    20641 caccgtcttt tttgagaatc cctgtgattt ctgtgaaggc accccgtgtt ccagagccat
    20701 tttctcgtga aatcacctca atcgttcctg aagctgactg tttggaagca gctgactgat
    20761 tgccacaggc aacaagccca aatcctgata agccaatggc tgcaagagta agcatttttt
    20821 tgaatttcat aataatcacc tttatctcta tgtatttttc ttgtgtaggc ttactacatt
    20881 tatagtctaa caagtctttg taaaggttta tccctgattc atgtaaagat tgtgtaaaga
    20941 atcaaaaaaa gccacttttg aaaaatggct gctcctaaaa atagctttaa aaattattag
    21001 tcctgtgcga aagattggtt aggaagaaaa atcgtgaagc aactgcctct gccaagctga
    21061 ctcgtcaccg tgacttggcc acctaataat tgactgagtt ctttgacaat ggcaaggcca
    21121 agaccagtgc caccagtttg tctgcttcga cctttattaa ctcggtaaaa acgttcaaaa
    21181 atacgatcct gctctaattg actaatacca atccctgtat ctgatacaga aatcttaatg
    21241 ccttcgttca ccttttgggt cttgacctca atttttcccc cttgttcagt gtaacggatg
    21301 gcattggata aaagattgag taagatttgg gaaagtaatt gactatctga tacgagggtg
    21361 acatcatctg gcacctgcac ctttagctgt aaatccttct tcttgagctg aggttgcaag
    21421 ctttgagtca aatcctgtac aaattctgcc aaagaaaggg tcgtccattg tataggcatt
    21481 tgttgagcct tagataaggt aagaagatgc tcaacaatat gctcaagacg caaactttct
    21541 ttgtaaataa tgtctagaaa gtcatccttg agcgcttctt cttcagctga catcccctta
    21601 atggtttcag caaagccctt aatcgaagta actggtgtcc tcaattcatg ggaggcattt
    21661 gagacaaagg ctaaatttaa cttttcataa gttctaatcg ttgttaaatc atatagcaag
    21721 acgagcacag cttccacaga ttgggtgggg ctaaaaacgg gaactgctgt cacttctaaa
    21781 atcaagtcac cctcatgaaa cccacttact tcttgtttta accttgtttt ttgatcaaag
    21841 gcttggtgaa ctaaatttcg aatatccatc cgtttgaggt catcaagtga acttatgtcg
    21901 ccgtccacat cgggaaaata atgaggcaga gagcgactgg ataataacat ctgaccttga
    21961 gcggaaacta aaaacgtccc catggttagg tgcgacagaa gaacctccat tgtttcggct
    22021 aggtccttgt attgctgatc ctgttgggag actttggttt ttagggcaga cacatactga
    22081 gccaaagact ttaagtcttc ttgccctttt tctaaaaagt attcactact ggtcaagaga
    22141 ggttggtgca aggtctcaaa agcaacttcc catttccaaa ggcaaaagag ccagtagcca
    22201 ccttgtccca aagaaagggc tagaagaaag agaccgatgc ctttactgat ccaagttaat
    22261 gccatccctg caatcagaat gaggctaaca cttagattga ctagccaaaa ttgaaggtag
    22321 cgtttcatct ataactcctt gaacttataa ccataacccc gaatggttcg aataaattga
    22381 ggggctttag gattgtcttc aattttttcc ctcaacttac caatatgaac gtccaccaaa
    22441 cgtgtttcct gcccaaagtc atacccccag atacgttcca aaagacgctc tctagtcagt
    22501 gtcatgttgg gatgtttcat aagatagagc aagagttcaa attcttttgg ggtcaaactc
    22561 agtaacttat tcgccttgta gacttcatga cgctcagggt atactttcaa ggtcccaaat
    22621 agccaagaat cgtcagcgat attatctgaa tcatctcctt cttgttctcc tttagttcgc
    22681 ctgaggacag ccttgacacg cgccagcaat tctctagggc taaaaggctt ggtcaggtag
    22741 tcatcagccc ctaattccaa ggccaaaacc ttatcaaatt catcactttt cgcagaaacc
    22801 atcataattg gagttttgac gcctttggct ctcagccgct tacaaacttc catgccatct
    22861 aattgtggta acatgatatc aagcaagata aaatcaaagg gttctgtttc tgccaaagct
    22921 aaggccttcc gtccatttgt taccaattga gtagaaaagc cttccttact taaatggtag
    22981 tcaagcaatt tcagaatgtg ttcttcatca tccactaata agacttgttt tgtcatctat
    23041 tatctcctat tggtaacatt ataacacaat tatcagaaat cctaacattg ctaaatcaga
    23101 ttaaatttgc ttatcaagac tagtatctgg tcaaacgctc aatcatctcc ttgtgctctg
    23161 gataggtcgc cagtagatct accctttcaa ataattcaaa atcctcaaat tcaaaaccag
    23221 gagcaacaag acaagaaacc agagcatcat ccttatcaac tgttgatccc caaatagtgc
    23281 ccttaggaac acagtagtga agttgttgcc ctttggatat gtccaggcct aaagtgactg
    23341 cttcgtagtg accatctgct gtgatcatgt gaacagtaag tggggatcct gcatgaaaat
    23401 accagatttc atctgctgtc aatcggtgaa aatgtgaagg attcgtttct tcttaataag
    23461 aaataaatac tggtataaag cgcccttccc ttaccagcaa ggtttatagt gtctgaagct
    23521 ttttttgttt gtctaaaata gccaccttca acatggggag ctaactctag agttcttatc
    23581 aagtcttctt tatccgtcgg agccaatggg ttgaagtaac tcttgttcaa agtgatttta
    23641 cgatttcaag aactcctctc agttctgagg acacggtaat gattgatgcg acggaagtac
    23701 aaatcaatcg ccctaaaaaa agaattagcg aatcattctg gtaaaaaaat gccacgctat
    23761 gaaggatcaa gcgattgtca caagtcaagg gagaattgtt tctttggata tcgctgtgaa
    23821 ctattgttat gatatgaagt tgttcaaaat gagtcgcaga aatatcggac aaactggtaa
    23881 aatcttggct gacagtggtt atcaagggct catgaagata tatcctcaag cacaaactcc
    23941 acgtaaatcc agcaaactca agccgctaac agttgaagat aaagcctata accatgcgct
    24001 atccaaggag agaagcaagg tcgagaacat ctttgccaaa gtaaaaacgt ttaaaatgat
    24061 ttcaacaacc tatcgaaatc atcgtaaacg cttcggatta cgaatgaatt tgattgctgg
    24121 tattatcaat catgaactag aattctagtt ttgcaggaag tctattattt ggttaggtga
    24181 attagtgaag cgtttaggca agtgtctctg gttacgacgt catggcctct aaatcgatta
    24241 tatttagggg tcatgactag tgaagcagtt agctagttcg catataagcg gctagcgtct
    24301 aacaattagg aactttagtt ccaataactt taagattatg acgttttagg acataaatcg
    24361 atcatattta tgtcctaaaa ctagtgaagc gcctagccaa agtccgaata ggatttggcg
    24421 ttagttactt agattgcttt gcaatcaagt aactttggcg atttacatct tctctggcgc
    24481 ttctactcca agcaagcgaa gggcttcttt gagaacgact gcggttgcgt agctgagggc
    24541 tagacggctg tcgcgttctg ggctttcatc caagatacgt gtatgtgcat agtatttgtt
    24601 aaaggattga gccaggctaa ttgcaaattt agcaatgata gaaggttcaa agttatctgc
    24661 cgcacggttg ataatacgtg ggaagtcttg aatgagttta atgatttccc agctttcagt
    24721 atcattcaag ctatagttgc cagctatttc tggtttgaaa tcggctttgc gtaagataga
    24781 ttggatacga gcgtaggcat attgaacgta aggtccagtt tcaccctcga aggataccat
    24841 agcttctagg tcgaagtcgt atccatttgt acggtcagtt ttgaggtcat agaatttaat
    24901 ggctccaacc ccaacagcat gtgctacttg gtctttgttt tctagttcag gatttttagc
    24961 ctcgatttgg accttggcac ggctaacagc ctctgcaaca gtaggctcta gcaagatgac
    25021 attcccttta cgagtagaga gcttcttccc ttcttttgta accagaccaa aaggaacgtg
    25081 ggtaatgtcg tcactccaat cgtagcccat ctcttgcaag acagctttga gttgtttaaa
    25141 gtgggcagat tgctcttgac caacgacgta gatagattta gcaaattggt attcattttt
    25201 acggtagagg gctgcagcca agtcacgtgt gatatagaga gttgcaccat cagacttctt
    25261 gatgagggct ggatgttcaa ttccatattt ctcaagattc acaacttggg caccttctga
    25321 ttcaagaagt agtccttttt cagaaagaat gtctacaact gcatccatct tatcattgta
    25381 gaaggcttct ccgttatagc tgtcaaattc aaccttcaat tcattgtaaa ggcggttaaa
    25441 ttccactaaa ctttcatcgc ggaaccattg ccaaagagcg agagcttcct catctccatt
    25501 ttcaagttta cggaaccatt cgcgcgcttc ttcatccaag ctagggtcat tttcagcttc
    25561 agcgttgatg cggacataga gtttaaggag ttcatcgatt ggatgagctt ttacagcttc
    25621 ttcgtcgccc cattttttgt aggcaacaat caacatccca aattgtttac cccagtctcc
    25681 caaatggttg accttgaccg tttgataacc gattttttgg aaaatatgtg acaagctatc
    25741 tccgataaca gttgaacgca ggtggccaat agaaaatggt ttagcgatat tcggactaga
    25801 catgtcgata acaacatttt cttgtttgcc aatattttgg tcagcatagt gttctttttc
    25861 agtggtaaca gcttgcaata cttgagcaga aatggcagat ttatcaagga aaaagttaac
    25921 gtaaggtcct gttgcgacaa ctttttcaaa ggcttggctg ttcatttttt cagccagttc
    25981 agccgcaatc atttgtggtg ctttacgttc gacttttgca agagaaaaag cagggaaagc
    26041 aatgtctccc atttctgagt ttttaggggt ttccagtaac tttaaaatag cctcttggtc
    26101 caggctatca atgatgctag ataattcgct agcaatcaat tcttttgtat tcattaagag
    26161 ctcctttttg gacttttcta ctattttatc acaattttaa agaaagaaga aaaaattttt
    26221 gaaatctcct gtttttttgg tataatatgg ttataaatat ggttataaat atgcacgcaa
    26281 gaggatttta tgagaaaaag agatcgtcat cagttaataa aaaaaatgat tactgaggag
    26341 aaattaagta cacaaaaaga aattcaagat cggttggagg cgcacaatgt ttgtgtgacg
    26401 cagacaacct tgtctcgtga tttgcgcgaa atcggcttga ccaaggtcaa gaaaaatgat
    26461 atggtgtatt atgtactagt aaatgagaca gaaaagattg atttggtgga atttttgtct
    26521 catcatttag aaggtgttgc aagagcagag tttaccttgg tgcttcatac caaattggga
    26581 gaagcctctg ttttggcaaa tattgtagat gtaaacaagg atgaatggat tttaggaaca
    26641 gttgctggtg ccaatacctt attggttatt tgtcgagatc agcacgttgc caaactcatg
    26701 gaagatcgtt tgctagattt gatgaaagat aagtaaggtc ttgggagttg ctctcaagac
    26761 ttatttttga aaaggagaga cagaaaatgg cgatagaaaa gctatcaccc ggcatgcaac
    26821 agtatgtgga tattaaaaag caatatccag atgctttttt gctctttcgg atgggtgatt
    26881 tttatgaatt attttatgag gatgcggtca atgctgcgca gattctggaa atttccttaa
    26941 cgagtcgcaa caagaatgcc gacaatccga tccctatggc gggtgttccc tatcattctg
    27001 cccaacagta tatcgatgtc ttgattgagc agggttataa ggtggctatc gcagagcaga
    27061 tggaagatcc taaacaagca gttggggttg ttaaacgaga ggttgttcag gtcattacgc
    27121 cagggacagt ggtcgatagc agtaagccgg acagtcagaa taattttttg gtttccatag
    27181 accgcgaagg caatcaattt ggcctagctt atatggattt ggtgacgggt gacttttatg
    27241 tgacaggtct tttggatttc acgctggttt gtggggaaat ccgtaacctc aaggctcgag
    27301 aagtggtgtt gggttatgac ttgtctgagg aagaagaaca aatcctcagc cgccagatga
    27361 atctggtact ctcttatgaa aaagaaagct ttgaagacct tcatttattg gatttgcgat
    27421 tggcaacggt ggagcaaacg gcatctagta agctgctcca gtatgttcat cggactcaga
    27481 tgagggaatt gaaccacctc aaacctgtta tccgctacga aattaaggat ttcttgcaga
    27541 tggattatgc gaccaaggct agtctggatt tggttgagaa tgctcgctca ggtaagaaac
    27601 aaggcagtct tttctggctt ttggatgaaa ccaaaacggc tatggggatg cgtctcttgc
    27661 gttcttggat tcatcgcccc ttgattgata aggaacgaat cgtccaacgt caagaagtag
    27721 tgcaggtctt tctcgaccat ttctttgagc gtagtgactt gacagacagt ctcaagggtg
    27781 tttatgacat tgagcgcttg gctagtcgtg tttcttttgg caaaaccaat ccaaaggatc
    27841 tcttgcagtt ggcgactacc ttgtctagtg tgccacggat tcgtgcgatt ttagaaggga
    27901 tggagcaacc tactctagcc tatctcatcg cacaactgga tgcaatccct gagttggaga
    27961 gtttgattag cgcagcgatt gctcctgaag ctcctcatgt gattacagat gggggaatta
    28021 tccggactgg atttgatgag actttagaca agtatcgttg cgttctcaga gaagggacta
    28081 gctggattgc tgagattgag gctaaggagc gagaaaactc tggtatcagc acgctcaaga
    28141 ttgactacaa taaaaaggat ggctactatt ttcatgtgac caattcgcaa ctaggaaatg
    28201 tgccagccca ctttttccgc aaggcgacgc tgaaaaactc agaacgcttt ggaaccgaag
    28261 aattagcccg tatcgaggga gatatgcttg aggcgcgtga gaagtcagcc aacctcgaat
    28321 acgaaatatt tatgcgcatt cgtgaagagg tcggcaagta catccagcgt ttacaagctc
    28381 tagcccaagg aattgcgacg gttgatgtct tacagagtct ggcggttgtg gctgaaaccc
    28441 agcatttgat tcgacctgag tttggtgacg attcacaaat tgatatccgg aaagggcgcc
    28501 atgctgtcgt tgaaaaggtt atgggggctc agacctatat tccaaatacg attcagatgg
    28561 cagaagatac cagtattcaa ctggttacag ggccaaacat gagtgggaag tctacctata
    28621 tgcgtcagtt agccatgacg gcggttatgg cccagctggg ttcctatgtt cctgctgaaa
    28681 gcgcccattt accgattttt gatgcgattt ttacccgtat cggagcagca gatgacttgg
    28741 tttcgggtca gtcaaccttc atggtggaga tgatggaggc caataatgcc atttcgcatg
    28801 cgaccaagaa ctctctcatt ctctttgatg aattggggcg tggaactgca acttatgacg
    28861 ggatggctct tgctcagtcc atcatcgaat atatccatga gcacatcgga gctaagaccc
    28921 tctttgcgac ccactaccat gagttgacta gtctggagtc tagtttacaa cacttggtca
    28981 atgtccacgt ggcaactttg gagcaggatg ggcaggtcac cttccttcac aagattgaac
    29041 cgggaccagc tgataaatcc tacggtatcc atgttgccaa gattgctggc ttgccagcag
    29101 accttttagc aagggcggat aagattttga ctcagctaga gaatcaagga acagagagtc
    29161 ctcctcccat gagacaaact agtgctgtca ctgaacagat ttcactcttt gatagggcag
    29221 aagagcatcc tatcctagca gaattagcta aactggatgt gtataatatg acacctatgc
    29281 aggttatgaa tgtcttagta gagttaaaac agaaactata aaaccaagac tcactagtta
    29341 atctagctgt atcaaggaga cttctttgac aattctccac ttttttgcta gaataacatc
    29401 acacaaacag aatgaaaagg agctgacgca ttgtcgctcc cttttgtcta ttttttaagg
    29461 agaaagtatg ctgattcaga aaataaaaac ctacaagtgg caggccctgg cttcgctcct
    29521 gatgacaggc ttgatggttg ctagttcact tctgcaaccg cgttatctgc aggaagtctt
    29581 aggcgccctc cttactggga aatatgaagt tatttatagt atcggggctt ggttgattgg
    29641 tgtggccgta gtcggtctag ttgctggtgg actcaatgtt gtcctcgcag cctatattgc
    29701 ccaaggagtt tcatccgacc ttcgggagga tgccttccgt aaaattcaaa ccttttctta
    29761 tgctgatatt gaacaattta atgcgggaaa tctagtcgtt ctaatgacaa atgatatcaa
    29821 ccagattcag aacgttgtca tgatgacctt ccaaattctt ttcagacttc ccctcttgtt
    29881 catcggttcg tttatcctag cggttcaaac cttaccttct ctgtggtggg tgattgttct
    29941 catggtagtc ttgatttttg gtttgactgc tgtcatgatg ggaatgatgg ggcctcgttt
    30001 tgccaagttt caaacccttc ttgagcgcat caatgccatt gccaaggaaa atttacgtgg
    30061 cgttcgtgtg gtcaagtcct ttgtccaaga aaaagagcaa tttgctaagt ttacagaggt
    30121 ctcagatgag cttcttggtc aaaaccttta cattggttat gccttttcag tagtggaacc
    30181 ctttatgatg ttggttggtt acggggcggt cttcctctct atttggctgg tcgcgggaat
    30241 ggttcagtcg gatccgtctg ttgttggttc catcgcttct tttgttaatt acctaagcca
    30301 gattatcttt accattgtta tggttggatt tttgggaaat tctgtcagcc gtgccatgat
    30361 ttccatgcgt cgtattcgag aaattcttga cgcagagcca gctatgacct tcaaggatat
    30421 cccagatgaa gagttggttg gaagtcttag ctttgaaaat gtgaccttta cctatccaat
    30481 ggacaaggaa ccgatgctga aagatgtgag ctttactatt gaacctggtc aaatggttgg
    30541 tgtagttgga gcgactggtg caggaaagtc aaccttggct caattgattc cacgtctctt
    30601 tgatccacag gacggggcca ttaaaatcgg tggcaaggat attcgagaag tgagtgaagg
    30661 aaccctgcgt aaaacagttt ccatcgttct ccaacgtgcc attcttttta gtggaacgat
    30721 tgcagataac ttgagacagg ggaaggggaa tgctactcta tttgaaatgg agcgcgcagc
    30781 caatattgcc caggctagtg aattcattca tcgtatggag aaaacctttg aaagtccagt
    30841 tgaagaacgg ggaaccaatt tctctggtgg acaaaaacaa aggatgtcga ttgcgcgtgg
    30901 gattgtcagc aatccacgta ttctgatttt tgacgattcg acctcagcct tggatgccaa
    30961 atcagagcgc ttggtgcaag aagctttgaa taaggacttg aaggggacga caaccattat
    31021 tattgctcaa aaaattagct cggttgtcca tgcagacaag atcttggttc taaatcaagg
    31081 acgattgatt ggtcaaggca cgcatgcaga cttggttgcc aacaatgccg tttaccgtga
    31141 aatctatgaa acacagaaat gaaagacaaa ctataagaaa agtcaatagt tttatctaaa
    31201 ttatttctta tttcaatttg atgatttggc gatgatttta gagcacggca aaaggccctt
    31261 gaaaaagtcc attttttcaa aggtaatcct gtgttaattt cagaaattac atcacttttt
    31321 gttcgtcaaa tggcagctct ttttttagga tataaaacag ggttcggata agtttttttg
    31381 caaggtggat gatggctaca ttgtaatgtt ttccttgttc taatttagtc ttaagatagg
    31441 ccttaaaagc aggcgaaaag cgagggcatg ctttggcagc ttgtatgagt acctaccgca
    31501 gatgagggga actccgtttg accattcttc ctgctaaatc aatctgatct gactgataaa
    31561 tagaagaatc cagtccagcg aaagcttgta attgagcagg attatcaaag gcatgaatat
    31621 ttcgaatctc agctaaaatg accgccccta aacgatcccc aatcccagta accgtcgtga
    31681 tgaccgagtt gaactcagcc atcaagtcat tgacacatgt ttccgccttg tcaatgagcc
    31741 tcttgtaatg tttgatgttt tcattacacg agataaaacg tctatgcgtt atcaaactca
    31801 ttaccaatta aaacaaaaag ctgtggttag atcctttcgg aaattgtcaa gcgattggag
    31861 gaaatgaact aatccacagc ggcttattcc aagtatacca cttgggcttt ggcagtagct
    31921 aactgcgcta aatataatat aaggaggagt aaaatgaaga cagttcaatt tttttggcat
    31981 tattttaagg tctacaagtt ctcatttgta gttgtcatcc tgatgattgt tctggcgact
    32041 tttgcccaag ccctctttcc agtcttttct ggacaagcgg tgacgcagct agccaattta
    32101 gttcaagctt atcaaaatgg caatccagaa cttgtatggc aaagcctatc aggaatcatg
    32161 gtcaatcttg gcctgctggt tttggttcta tttatctcta gtgtaatata catgtgtctc
    32221 atgacgcgcg tgattgcaga atcgaccaac gagatgcgca aaggcctctt tggtaagctt
    32281 gctcagttga cggtttcttt ctttgaccgt cgacaagatg gcgatatcct gtctcatttt
    32341 accagtgatt tggataatat cctccaagcc tttaacgaaa gcttgattca ggtcatgagc
    32401 aatattgttt tatacattgg tctgattctt gtcatgtttt cgagaaatgt gacgctggct
    32461 ctcatcacca ttgccagcac cccattggct ttccttatgc tgattttcat cgtgaaaatg
    32521 gcacgcaaat acaccaacct ccagcagaaa gaggtaggga agctcaacgc ctatatggat
    32581 gagagcatct caggccaaaa agccgtgatt gtgcaaggaa ttcaagagga tatgatggca
    32641 ggatttcttg aacaaaatga gcgcgtgcgc aaggcaacct ttaaaggaag aatgttctca
    32701 ggaattcttt tccctgtcat gaatgggatg agcctgatta atacagccat cgtcatcttt
    32761 gctggttcgg ctgtactttt gaatgataag tctattgaaa caagtacagc cctaggtttg
    32821 attgttatgt ttgcacaatt ttcacagcag tactaccagc ctattatcca agttgcagcg
    32881 agttggggaa gccttcagtt ggcctttact ggagctgaac gaattcagga aatgtttgat
    32941 gcagaggagg aaatccgacc tgaaaaggct ccaaccttca ctaagttgca agaaagtgtt
    33001 gaaatcagtc atatcgattt ttcatacttg cctgataaac ctattttgaa agatgtcagc
    33061 atttctgccc ctaaaggcca gatgacagca gttgttgggc cgacaggttc aggaaaaacg
    33121 actattatga acctcatcaa tcgcttttat gatgttgatg ctggtggtat ttattttgat
    33181 ggtaaagaca ttcgtggcta tgacttagat agtcttagaa gcaaggtggg aattgtattg
    33241 caagattcgg tcttgtttag cggaacgatt cgagacaata tccgatttgg tgtgccagat
    33301 gctagtcagg aaatggttga ggtagcagca aaagcaaccc acattcacga ctatatcgaa
    33361 agtttgcctg ataagtacga tactcttatt gatgatgacc agagcatctt ttcaacaggg
    33421 cagaagcaat tgatttcaat cgctcgaacc ctgatgacag atccagaagt tctcattctc
    33481 gatgaagcaa cttcaaacgt agatacggtg acagaaagca agattcagca tgccatggag
    33541 gtggttgtag caggtagaac tagtttcgtc attgcccacc gcttgaaaac cattctcaat
    33601 gcagatcaga ttattgtcct taaagatgga gaagtcattg aacgtggtaa ccaccatgaa
    33661 cttttgaagc taggtggctt ttattcagaa ctctatcaca atcaatttgt tttcgaataa
    33721 gaaagaagtt gtcctatgtg ggcagctttt tcttgtccat aaaaaatgtt tatcacagcc
    33781 ttaaaaaaaa catattagac gaaagtcatt ttgagtgata tgataggact atcgttagca
    33841 ttcgaaagga gaggtatcat ggctagaacg gttgtaggag ttgctgcaaa tctatgtccc
    33901 gtagacgcag aaggcaaaat cattcattca tctgtatctt gtagattcgc agagatcatt
    33961 cgtcaagtcg gtggtctccc tttagtcatt cctgttggtg atgagtcagt tgtacgtgat
    34021 tatgtggaaa tgattgacaa actcattttg acaggaggcc aaaatgttca tcctcagttt
    34081 tatggagaga aaaagaccgt cgagagcgat gattacaatc tggtccgtga cgaatttgaa
    34141 ttggcactct tgaaggaagc gcttcgtcag aataaaccaa ttatggcaat ctgtcgcggt
    34201 gtccaacttg tcaatgttgc ctttggtgga accctcaatc aagaaatcga aggtcactgg
    34261 caaggactac ctttcgggac atctcactct attgagacag tagaaggaag cgtggtggct
    34321 aagctatttg ggaaagaaag tcaggtcaac tcagtccatc gtcaaagcat taaagatttg
    34381 gcacctaatt tccgtgtaac tgctattgat tcaagagacc aaactattga agcgattgag
    34441 tctatcgacg agcaccgcat tatcggttta cagtggcatc cagagtttct ggttaatgaa
    34501 gaagatggca atttagaatt atttgagtat ttattgaatg aactgtaacg attggaacat
    34561 ctggtcgttc tttcttttat tttttcaaaa tttttagaat gtggcatttt acgcaaacgt
    34621 ttgaattctg ataaaaattg gagaaattcg acaaaaaaac ttgaaaaaaa cgaaggtaag
    34681 cgttatgata gaaaagaaga aatattggag gaagaacatg tcacatatta aatttgatta
    34741 ttcaaaagtt ttagacaaat ttgttgcacc acatgaagtg gaatacatgc aatcacaagt
    34801 aacagcagca gatgaattaa tccgtaaagg aactggtgct ggtagcgact tcttgggatg
    34861 gttggacctt cctgaaaaat acgaccgcga agaatttgac cgcatcttga aagctgctga
    34921 gcaaatcaag tcagacagcg atgttttggt tgtgatcggt attggtggat cttaccttgg
    34981 tgcaaaagca gcaatcgact tcttgaatca ccattttgct aacttgcaaa caaaagaaga
    35041 acgcaaagct ccacaaatcc tttacgcagg aaactcaatc tcatctactt accttgctga
    35101 cttggtagag tatgttgcag acaaagactt ctcagtaaac gtaatttcta aatcaggtac
    35161 aacaactgaa ccagcgattg ctttccgtgt ctttaaagaa ctcttggtta agaaatacgg
    35221 tcaagaagaa gctaacaaac gtatctatgc aacaactgac cgccaaaagg gtgctgttaa
    35281 ggttgaagca gacgctaacg gttggggaac atttgttgtt ccagatgata tcggtggacg
    35341 cttctcagta ttgacagccg ttggtttgct tccaatcgca gcatcaggag ctgacataaa
    35401 agctcttatg gaaggtgcga atgcagctcg caaagactac acttcagaca aaatctctga
    35461 aaacgaagct taccaatacg cagctgttcg taacatcctt tatcgtaaag gctatgcaac
    35521 tgagatcttg gtaaactatg agccatcact tcaatacttc tcagaatggt ggaaacaatt
    35581 ggctggtgaa tcagaaggaa aagaccaaaa aggtatctac ccaacttcag ccaacttctc
    35641 aactgacttg cactcacttg gtcaatttat ccaagaagga actcgtatca tgtttgaaac
    35701 agttgtccgt gttgacaaac ctcgtaaaaa cgtgcttatt cctactttgg aagaagacct
    35761 tgacggactt ggttaccttc aaggaaaaga cgttgacttt gtaaacaaaa aagcaactga
    35821 cggtgttctt cttgcccaca cagatggtga tgtaccaaac atgtatgtga ctcttccaga
    35881 gcaagacgct ttcactcttg gttacactat ctacttcttc gaattggcaa ttgccctttc
    35941 aggttacttg aatgctatca acccatttga ccaaccaggt gttgaagctt ataaacgtaa
    36001 catgtttgcc cttcttggaa aaccaggatt tgaagaattg agcaaagaac ttaacgcacg
    36061 tctataatag aagaaaagag tggtttgccc actcttttta ctctctttat ccatagaaat
    36121 tggactcagc caagacttgt gatataatat agaaagcaaa aaggcagacg cctagataat
    36181 aggagaaact atgtcaaaag atatccgcgt acgttacgca ccaagtccaa caggactact
    36241 acacatcgga aatgctcgta cagcattgtt taattacttg tatgcgcgcc atcatggtgg
    36301 aacatttctc atccgtatcg aagatactga ccgtaaacgc catgtcgagg atggtgaacg
    36361 ttcacaactt gaaaaccttc gctggttagg catggattgg gatgaaagtc cagaatcaca
    36421 tgagaattat cgccagtctg agcgtttgga cttgtatcaa aaatatattg accaactatt
    36481 agctgaagga aaagcctata aatcttacgt tacagaagaa gagttggcag ctgaacgcga
    36541 acgccaagaa gtagctggcg aaacaccacg ctacatcaat gaataccttg gtatgagtga
    36601 agaagaaaaa gcagcttaca tcgcagaacg tgaagcagca gggatcatcc caactgttcg
    36661 tttggctgtc aatgagtcag gtatctacaa gtggcatgat atggtcaaag gcgatatcga
    36721 atttgaaggt ggcaatatcg gtggtgactg ggttatccaa aagaaagacg gttacccaac
    36781 ttacaacttt gccgttgtta tcgatgacca cgatatgcaa atctctcatg ttatccgtgg
    36841 agatgaccat attgctaata caccaaaaca gcttatggtc tatgaagctc ttggttggga
    36901 agctccagag ttcggtcaca tgaccttgat tatcaactct gagactggta agaagttatc
    36961 taaacgtgat actaatacac ttcaatttat cgaagattac cgaaagaaag gttaccttcc
    37021 agaagcagtc tttaacttta ttgctcttct tggttggaac ccaggtggtg aagatgagat
    37081 tttctctcgt gaagaattca ttaaactttt tgatgaaaac cgcctcagca agtcaccagc
    37141 agcctttgat cagaaaaaac tagactggat gagcaatgat tatatcaaga atgcagacct
    37201 tgaaaccatc tttgaaatgg caaaaccatt cttagaggaa gcaggccgtt tgactgacaa
    37261 ggctgaaaaa ttagttgagc tctataaacc acaaatgaaa tcagtagatg agattatccc
    37321 attgacagat cttttcttct cagatttccc agaattgaca gaagcagagc gcgaagtcat
    37381 gacgggtgaa acagttccaa cagttcttga agcattcaaa gcaaaacttg aagcgatgac
    37441 agatgatgaa tttgtgacag aaaatatctt cccacaaatt aaagcagtcc aaaaagaaac
    37501 aggtattaaa gggaaaaatc ttttcatgcc tattcgtatc gcagtttcag gcgaaatgca
    37561 tgggccagaa ttaccagata caattttctt gcttggacgt gaaaaatcaa ttcagcatat
    37621 cgaaaacatg ctaaaagaaa tctctaaata aagaggatac aataatggac atctgggaaa
    37681 agatgtacga agaagcacag aaactataca atccacatga agtatcagac tttgtttatg
    37741 ccaatcatgt tgtagctgca gtagaagcag gagatggaca aatatttaca ggattctgta
    37801 tggagggaac ctgtggtgtt ttccatctct gcgcagaacg ggcggccctc ttcaatatgt
    37861 accaattttc aggacaaact aaggttaaga aagtattagc ctttcgagac aaaccacctt
    37921 atggtggaag ttcagccatg ccttgcggtg cttgtagaga attcctttta gagcttaatg
    37981 ctgaaaataa agatgcagaa ttcatgatgg actataatat aagaaaaaca gttaaagtcg
    38041 cagaactaat cccttattgg tggggagaag aacgtgcttc taagtttaat gagcaataga
    38101 agagtcagta taattctggc tctttttact ataagtttca aaaaaaatga aaaaaattag
    38161 aaaaagtagt tgacaaagtt tgaaaagact gtataatagt aagagttgaa aataacaact
    38221 caggtccgtt ggtcaagggg ttaagacacc gccttttcac ggcggtaaca cgggttcgaa
    38281 tcccgtacgg actatggtat gttgcggttg gaacacttga tgaaaaaaag tttaaaaaaa
    38341 cttaaaaatc ttcaaaaaag tgttgacaag tgaaagtggc tgtgatatac taatatagtt
    38401 gtcgcttgag agaagcaagt gacaaagacc tttgaaaact gaacaagacg aaccaatgtg
    38461 cagggcgcta caacgtaagt tgtagtactg aacaatgaaa aaaacaataa atctgtcagt
    38521 gacagaaatg agtgagaact caaa
//
LOCUS       DAVKJJ010000015        38130 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000015 DAVKJJ010000000
VERSION     DAVKJJ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38130
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.015"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(105..2363)
                     /locus_tag="VW144_000758"
     CDS             complement(105..2363)
                     /locus_tag="VW144_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9600237.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVNLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2592..2822)
                     /locus_tag="VW144_000759"
     CDS             complement(2592..2822)
                     /locus_tag="VW144_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEU9600238.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3012..3695
                     /locus_tag="VW144_000760"
     CDS             3012..3695
                     /locus_tag="VW144_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9600239.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3695..4429
                     /locus_tag="VW144_000761"
     CDS             3695..4429
                     /locus_tag="VW144_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9600240.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4583..5440
                     /locus_tag="VW144_000762"
     CDS             4583..5440
                     /locus_tag="VW144_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEU9600241.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLAHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5582..6454
                     /locus_tag="VW144_000763"
     CDS             5582..6454
                     /locus_tag="VW144_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU9600242.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPESMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            6736..7419
                     /gene="rpiA"
                     /locus_tag="VW144_000764"
     CDS             6736..7419
                     /gene="rpiA"
                     /locus_tag="VW144_000764"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU9600243.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7433..8644
                     /locus_tag="VW144_000765"
     CDS             7433..8644
                     /locus_tag="VW144_000765"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEU9600244.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEADIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            8646..9188
                     /locus_tag="VW144_000766"
     CDS             8646..9188
                     /locus_tag="VW144_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEU9600245.1"
                     /translation="MTRYALLVRGINVGSKNKVVMAELHQELTNLGLEKVESYINSGN
                     IFFASIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            9204..10013
                     /locus_tag="VW144_000767"
     CDS             9204..10013
                     /locus_tag="VW144_000767"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9600246.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10489..10884
                     /locus_tag="VW144_000768"
     CDS             10489..10884
                     /locus_tag="VW144_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HEU9600247.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEITEPAQADDIRKVVGELAKDISITKLYMTGHSLGGYLAQIAAVEDYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWDAKNGF"
     gene            10894..11469
                     /locus_tag="VW144_000769"
     CDS             10894..11469
                     /locus_tag="VW144_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU9600248.1"
                     /translation="MESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKARFFKKQALPQSSSQPSAEPME
                     NIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDGSGKEIE"
     gene            11494..12006
                     /locus_tag="VW144_000770"
     CDS             11494..12006
                     /locus_tag="VW144_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEU9600249.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNGETAI
                     QVSKSVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVTVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            12660..13370
                     /gene="deoD"
                     /locus_tag="VW144_000771"
     CDS             12660..13370
                     /gene="deoD"
                     /locus_tag="VW144_000771"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9600250.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLGDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKELGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(13393..13917)
                     /locus_tag="VW144_000772"
     CDS             complement(13393..13917)
                     /locus_tag="VW144_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEU9600251.1"
                     /translation="MKITIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFLPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFDWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(13970..14206)
                     /locus_tag="VW144_000773"
     CDS             complement(13970..14206)
                     /locus_tag="VW144_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU9600252.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSTKLAK"
     gene            complement(14274..15194)
                     /locus_tag="VW144_000774"
     CDS             complement(14274..15194)
                     /locus_tag="VW144_000774"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEU9600253.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            15384..15787
                     /locus_tag="VW144_000775"
                     /pseudo
     CDS             15384..15787
                     /locus_tag="VW144_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16003..16584
                     /locus_tag="VW144_000776"
     CDS             16003..16584
                     /locus_tag="VW144_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9600254.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGEKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLAQRNAEKNNA
                     KATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLEIGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            16581..17859
                     /locus_tag="VW144_000777"
                     /pseudo
     CDS             16581..17859
                     /locus_tag="VW144_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
     gene            17877..18539
                     /gene="deoC"
                     /locus_tag="VW144_000778"
     CDS             17877..18539
                     /gene="deoC"
                     /locus_tag="VW144_000778"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU9600255.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYGFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSTVKAFETKEAIQNGADEIDMVINVGALKSGDL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKVLACQLAQKAGADFVKTSTGFSTGG
                     ATIADVRLMRETVGPDMGVKAAGGARSYADALTFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            18526..18915
                     /locus_tag="VW144_000779"
     CDS             18526..18915
                     /locus_tag="VW144_000779"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9600256.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            19006..20058
                     /locus_tag="VW144_000780"
     CDS             19006..20058
                     /locus_tag="VW144_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEU9600257.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQDWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            20202..21737
                     /locus_tag="VW144_000781"
     CDS             20202..21737
                     /locus_tag="VW144_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9600258.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQGLGVLMAGGNLGKEK
                     SDV"
     gene            21730..22788
                     /locus_tag="VW144_000782"
     CDS             21730..22788
                     /locus_tag="VW144_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9600259.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGFGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            22791..23747
                     /locus_tag="VW144_000783"
     CDS             22791..23747
                     /locus_tag="VW144_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9600260.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGVIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
     gene            23832..25181
                     /locus_tag="VW144_000784"
                     /pseudo
     CDS             23832..25181
                     /locus_tag="VW144_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(25224..25865)
                     /gene="plsY"
                     /locus_tag="VW144_000785"
     CDS             complement(25224..25865)
                     /gene="plsY"
                     /locus_tag="VW144_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU9600261.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            26001..27944
                     /gene="parE"
                     /locus_tag="VW144_000786"
     CDS             26001..27944
                     /gene="parE"
                     /locus_tag="VW144_000786"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEU9600262.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            28364..30835
                     /gene="parC"
                     /locus_tag="VW144_000787"
     CDS             28364..30835
                     /gene="parC"
                     /locus_tag="VW144_000787"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEU9600263.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLV
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEAILYVEVLDQFDDATTYFAATRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            30967..31989
                     /locus_tag="VW144_000788"
     CDS             30967..31989
                     /locus_tag="VW144_000788"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEU9600264.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            32045..32749
                     /locus_tag="VW144_000789"
     CDS             32045..32749
                     /locus_tag="VW144_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9600265.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     STTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            32814..33503
                     /locus_tag="VW144_000790"
     CDS             32814..33503
                     /locus_tag="VW144_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEU9600266.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            33500..34423
                     /locus_tag="VW144_000791"
     CDS             33500..34423
                     /locus_tag="VW144_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEU9600267.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            34438..35082
                     /gene="pcp"
                     /locus_tag="VW144_000792"
     CDS             34438..35082
                     /gene="pcp"
                     /locus_tag="VW144_000792"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU9600268.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            35250..35480
                     /locus_tag="VW144_000793"
     CDS             35250..35480
                     /locus_tag="VW144_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEU9600269.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            35530..35610
                     /locus_tag="VW144_000794"
     tRNA            35530..35610
                     /locus_tag="VW144_000794"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35564..35566,aa:Tyr,seq:gta)
     gene            35620..35691
                     /locus_tag="VW144_000795"
     tRNA            35620..35691
                     /locus_tag="VW144_000795"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35652..35654,aa:Gln,seq:ttg)
     gene            35853..37055
                     /gene="rpsA"
                     /locus_tag="VW144_000796"
     CDS             35853..37055
                     /gene="rpsA"
                     /locus_tag="VW144_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU9600270.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(37140..38130)
                     /locus_tag="VW144_000797"
                     /pseudo
     CDS             complement(37140..38130)
                     /locus_tag="VW144_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT        11433 a         6817 c         8625 g        11255 t
ORIGIN      
        1 actatatatc ataatggaca aagggcaagc tgtcaagaaa ttacagttat tttagtaaaa
       61 aaagaagcag aaaaatttca ttttctactc tttttctata ggatttattt ttctttttca
      121 gaacttttaa cttcagcttt tttggcagat ttaatctgaa tgtttccctt gctagtcata
      181 actgctttga gatctttttc gcttggattt tcaaggtagt agtcagtgat tgtgtcctca
      241 atatagtctt gaatagtccg acgaagtggg cgtgctccca tttttggatc ataacctagg
      301 tcaaccaact tttccttgac cttatcagtt acatccaaac gaatgttgtt gctagagagg
      361 cgcttgttaa catctgctag catgagctcg acaatctgaa ggaggttatc cttgctgaga
      421 gccttaaatt cgataatgcc atcaaaacgg ttcataaact ctgggctaaa gaagttaccg
      481 agttcaccga ggacagaatt ggtacgtcct tctctagcag caccaaatcc aacgctagct
      541 tcggtctttc ctgtacctgc atttgaggtc atgataatga tggcatcctt gaagctaacg
      601 gtgcgtcctt gcccgtctgt caaacgacca tcgtccaaga cttgaagaaa catgtgcata
      661 acatctgggt gagctttttc cacttcatcg agaaggatga gagaatatgg attgtggcga
      721 actttttcag ttaattgacc agcctcatca tagccaacat aacctggagg ggcgccgacc
      781 aacttagcca cactatgttt ttccatgtat tcactcatat caaagcgaat catactatca
      841 gcagaaccaa aaagttcgat agccagttgt ttggaaagtt ctgtcttacc gacaccagtt
      901 ggcccaacga agaggaagct tccgattggg cggttagggg taccaagtcc gacacgatta
      961 cggcgaatag ccttggcaat cttatcgact gcatcatctt ggccaataac atgagacttg
     1021 agatcttcgg ctagatggat gagttgagat tgttctttct ctttcaaatc accaacaggg
     1081 atattggttt tctgctcgat aatgtgctca atagttttct cgctgatgat aggagtatcc
     1141 tggtctgtga tctttttctt ttgcatttcc ttatacttgg caatctggtc gcggaagtag
     1201 gccgccttct caaaatcttc ttctcgtgta gcttgagact tgagattttc agcctcaatc
     1261 aagcgctgat caattacttt aggatccaca aaattcaagg tcaagttcat cttagaacca
     1321 gcttcatcta ggaggtcaat ggccttgtca ggcaagaagc gatcttggat gtagcgattg
     1381 gaaagagttg cagctgcttc aatcgcagca tctgtatatt gaacgtggtg gtaatcttcg
     1441 tatttctttt gaatcccttt gagaatagtg attgtttcgt ccaccgttgg ttcatcgact
     1501 ttaacaggct gcatacgacg ctcgagggca gcatcctttt caatgatacg gtattcattg
     1561 agggtagtag caccgactag ttgcagttct ccacgagcaa gggctggctt gaggatattt
     1621 cctgcgtcca tattaccatc actcgcagaa ccagcaccaa caatttcatg gatttcatcg
     1681 ataaagagga tgatgtcttc acgtttgcga atttcttcca tgagtttttg catgcgttct
     1741 tcaaattgtc ctcgaatccc cgttccttga actaagttaa ccacatccag acggatgact
     1801 tgtttacctt ggagtttatg tggcacatcg ccatcgacaa ttttctgagc tagaccttcg
     1861 acaacggccg tttttccgac accaggttca ccgataagga caggattatt cttggttcta
     1921 cgattgagaa tctcgatgac acggataatc tcatcgtcgc gcccaataac ggggtcaatg
     1981 tctccacgac gggcaatttc agttacatta ataccaaatt cttccagcag gcctttttct
     2041 tggctaggtg gcggagtttg agcagatcca cgattttggg aaccataacc gccgtttcca
     2101 ccgtaacctc cacctgattg ggttggggga ataggaggag tattgctaga aggtctgaaa
     2161 ttgtttagat cattgaagaa atcaccaaag ggatcgaagt cacgattgtt cagatccgtc
     2221 atacctttga agaggctatt gttaggatct gttttgataa tcttatagca gttttgacag
     2281 aggtcaattt gtttttgttt tccattgaga ttggtgtaaa gatgaattgt tgagtcgtta
     2341 attttacagt tttgacaaag catacatcta cctcatttct ttctttagcc tgacttgttt
     2401 aaaggtcaaa aatagtcaaa ttctatactc tcattataac aggattgggg tgtaaagcaa
     2461 gtaaaaagat tgaagctttg agaagttgtt acaatgaaaa tttttgtgaa tttggactat
     2521 aaaaaaatcc tatttgtgag acaaaaagga ttttacttag acatgcaggg acaatcccag
     2581 cttgttttag attagtagag aagttcgtaa atttccattg cgattaagtc aatgctatca
     2641 aacgtatacg tttcgcgatc ttctacgtca cgaagggtaa agattgggcc gttttcttcg
     2701 tcgtggctgt acgcaacttc tgcaacaaca actccttcgc gttcaaaatt acgttttaaa
     2761 tttccgccat cgtttgccat tgcttcaagg cggttgatga ttctaaccaa atgtgattcc
     2821 attttgattc tccttcattt cttatcgtta ctattttacc ataaaggagg ccaacttgac
     2881 tagaaaaaac taagatttct cgctaattct gctagtttaa agatttttaa ataaattgga
     2941 taaaaaattt aaattttaat tcaaaacatg ttataatcat acagtattct attttagaaa
     3001 gcagtgtgac tatgaatttt tcttttttac ctaagtattt accttatttt aactatgggg
     3061 ctgttgtgac gattcttatt tctatctgtg ttatcttttt gggaactatt ttgggtgttg
     3121 tcttggcttt tgggcaacgt tcaaagttta aaccgcttgt ttggttggcc aacttgtacg
     3181 tttggatttt ccgtgggaca ccgatgatgg ttcaaattat gattgccttt gctcttatgc
     3241 atatcaatgc tccgactatt cagattggaa ttttaggtgt tgatttttcg cgtctgattc
     3301 cagggatttt gattatctct atgaatagtg gtgcttatgt ttcggagact gttcgtgccg
     3361 gaatcaatgc ggttccaaaa ggtcagctag aggcggctta ttcgctaggg attcgtccta
     3421 aaaatgcgat gcgttatgtg attttgccac aagcagtcaa aaatatcttg ccagcattgg
     3481 ggaacgaatt tatcaccatt atcaaggaca gctccctctt atcagctatt ggggtcatgg
     3541 agttgtggaa tggggctaca acagtttcta caacaaccta tctaccttta acaccacttt
     3601 tatttgcagc attttactac ttgattatga cctctattct gacagtagcc ttgaaagctt
     3661 ttgaaaaaca tatgggacaa ggagataaga aataatgaca gaaaccttga taaaaattga
     3721 aaatttacat aaatcttttg gaaagaatga agtattgaag ggcatcaacc tcgagattaa
     3781 aagaggagaa gttgtcgtta tcatcggtcc ttcagggagt gggaaatcta ccttgcttcg
     3841 ctctatgaat ttgttggagg aagcaaccaa ggggaagctt atctttgagg gagtcgatat
     3901 tacggacaag aagaatgacc tgtttgccat gcgtgagaag atgggcatgg ttttccaaca
     3961 atttaacctc tttcctaata tgactgtgat ggaaaatatt accttgtcac ctatcaagac
     4021 taaaggtgaa agcagggaag ttgctgagaa gagagcccaa gagcttttgg aaaaagttgg
     4081 tttgccagat aaggcagacg cttatccaca gagtctgtcg ggtgggcaac aacaacggat
     4141 tgccatcgcg cgtgggttgg ctatggaacc agatgttttg ctctttgacg aaccaacttc
     4201 agccttggat cctgaaatgg taggtgaggt attggctgtt atgcaagacc ttgccaagtc
     4261 agggatgact atggttatcg taacacatga gatgggattt gcccgtgagg tggcagatcg
     4321 tgttatcttt atggcagacg gtgtggttgt tgaagatggg acacctgagc agatttttga
     4381 acaaacccaa gaacaacgga ctaaagattt cttgagtaag gttttataat ctattttaaa
     4441 atttcaagac aagattagtg aaaagttcaa ccagagcttt ttcttatagt ttgaaactat
     4501 aggataacct aggaaagaag tgttagaggg cactagcagt ttttggtata atggaagata
     4561 tttgaaagaa aagagaagtg atatgacaca gattattgat gggaaagctt tagcggccaa
     4621 attgcagggg cagttggctg aaaagactgc aaaattaaag gaggaaacag ctctagtgcc
     4681 tggtttggta gtgattttgg ttggggataa tccagccagc caagtctacg ttcgcaacaa
     4741 ggagaggtca gctcttgcgg ctggttcccg tagcgaagta gtgcgagttc cagagactat
     4801 tactcaagag gaattgttag acctgattgc taaatataat caggatccag cttggcatgg
     4861 gattttggtc cagttgccat taccaaaaca tatcgatgaa gaggcggttt tattagccat
     4921 tgacccagaa aaggatgtgg atggtttcca tcccctaaac atgggacgtc tttggtctgg
     4981 acatccagtc atgattccct cgacacctgc aggaattatg gaaatgtttc atgaatatgg
     5041 gattgacttg gaaggtaaaa atgcggtcgt aatcggtcgt tcaaatatcg ttggaaaacc
     5101 tatggctcag cttcttttgg ctaaaaatgc gacagtaacc ttggcccact cacgaactca
     5161 taatcttgcc aaggtggctg ctaaagcaga tattcttgta gtcgcaatcg gccgcgccaa
     5221 gtttgtgact gctgactttg tcaaaccagg tgcggttgtc attgacgttg ggatgaatcg
     5281 agatgaaaat gggaagctct gtggagatgt tgattatgag gcagttgcac cacttgctag
     5341 ccatatcacg ccagtccctg gaggtgtcgg tcctatgacc attaccatgc tgatggagca
     5401 aacttatcaa gcagcacttc ggacattgga tagaaaataa gagaaacatt cgattaatag
     5461 agtgtatttt ctatagctat atctaaaatg gcagaaatga atattaaatt ttagatataa
     5521 gattacaaaa ggaggtctgc gtctcctttt tattgtataa taaaagtgag aggaaaaaag
     5581 gatgaaagtg attaatcaaa ccttattaga aaaagttatt attgaacgtt ctcgttcaag
     5641 tcataaagga gattatggtc gtctgctgct gcttggtggt acttatcctt atggaggtgc
     5701 catcatcatg gctgctttag cagctgtaaa aagcggtgca ggattggtga ccgttggaac
     5761 ggacagggaa aatatcccgg ctctgcacag ccatttacct gagtctatgg ccttttctct
     5821 tcaagaccag caattgttaa aagagcaatt ggagaaggca gaagttgtct tgctggggcc
     5881 tggtttacga gacgatgctt ctggagaaaa tctagtaaaa caggtctttg ttaatttaag
     5941 ccaaaatcag attttgattg tagacggagg tgccttgacc attcttgcta ggacaagttt
     6001 gtcatttccc tcaagccagc ttatcctaac tcctcaccaa aaagaatggg aaaaattatc
     6061 tggaattacg attgaaaagc aaaaggaaga tgcaacggct agtgttctga cttccttccc
     6121 tcaaggaaca attttggtgg agaaaggccc agctactcgt atttgggaag ttggccaatc
     6181 tgattattac cagttacagg ttggtggtcc ttatcaggcg actgggggaa tgggagatac
     6241 tctggctggg atgatcgcag gatttgtagg ccaattccga caggccagtc tctacgagcg
     6301 tgtggcagta gcgacccatc ttcattcagc catagcccaa gaactatctc aagaaaatta
     6361 tgtggtctta ccgacggaaa tcagcaggta tcttcccaaa ataatgaaaa ttatttgtca
     6421 acaagagagg gtaagcaaag ataaacttgt ttaaaataga cagataaatc tacttccttc
     6481 tcttaattta tgattcttga aatgcgcctg ctttaaggct aaactagacg tatcaaagtt
     6541 aagagtttgt ttttgtaact ggaaagagat gaacaaatca aatacataaa agataaactc
     6601 atttttattc gtttggtaaa aataaaatgc atatttttaa agaaagattg atgaaaaatg
     6661 tagatttgat gtttttactt gaataaaaag cggtttcata gtaaaataag aattgagaat
     6721 gatggaggta aatcggtgga aaatctgaag aaaatggcag gtatcaaggc tgctgagttc
     6781 gtgagcgatg gaatggtcgt tggacttgga acaggctcga ctgcctatta ttttgtcgaa
     6841 gaaatcggtc gtcgtatcaa ggaagaagga ttgcagatta cagctgtaac aacttctagt
     6901 gtgaccacta aacaggcaga aggccttaat atcccgctca agtctattga ccaagtagac
     6961 tttgtcgatg tgacggttga tggcgcagat gaagtggata gtcagtttaa tggaatcaaa
     7021 ggcggtggtg gtgcccttct catggaaaag gtggtcgcaa caccatcaaa agaatacatt
     7081 tgggtggtgg atgaaagcaa gctggtcgaa aaactaggtg cttttaaatt gccagtagaa
     7141 gtggttcagt atggtgcaga gcaggtcttt cgtcattttg aacgagctgg ctacaaacca
     7201 agtttccgtg aaaaagacgg ccaacgtttt gtgacggata tgcagaattt tatcattgac
     7261 ctcgccttgg atgtcattga aaatccaatt gcttttggac aagaattgga ccatgtcgtt
     7321 ggtgttgtgg agcatggttt attcaaccaa atggtggata aggtaatcgt tgctggacga
     7381 gatggagttc agatttcaac ttcaaaaaaa ggaaaataga agggggcata agatgtctaa
     7441 atttaatcgt attcatttgg tggtactgga ttctgtagga atcggtgcag caccagatgc
     7501 taataacttt gtcaatgcag gggttccaga tggagcttct gacacactgg gacacatttc
     7561 aaaaacagtt ggtttgaatg tcccaaacat ggctaaaata ggtcttggaa atattcctcg
     7621 tgaaactcct cttaagactg tagcagctga aagcaatcca actggatatg caacaaaatt
     7681 agaggaagta tctcttggta aggatactat gactggacac tgggaaatca tgggactcaa
     7741 cattactgag cctttcgata ctttctggaa cggattccca gaagaaatcc tgacaaaaat
     7801 tgaagaattc tcaggacgca aggttattcg tgaagccaac aaaccttatt caggaacggc
     7861 tgttatcgat gattttggac cacgtcagat ggaaactgga gagttgatta tctatacttc
     7921 agctgaccct gttttgcaga ttgctgccca cgaagacatt attcctttgg atgaattgta
     7981 ccgtatctgt gaatacgctc gttcgattac ccttgagcgt cctgcccttc ttggtcgcat
     8041 cattgctcgc ccttatgtag gtgaaccagg taacttcact cgtacggcaa accgtcgtga
     8101 cttggctgta tctccatttt ccccaactgt tttggataaa ttgaatgagg ctgatatcga
     8161 tacttatgct gtgggtaaaa tcaacgatat ctttaacggt gctggtatca accatgacat
     8221 gggtcacaac aagtcaaata gtcatggaat tgatacacta ttgaagacta tgggacttgc
     8281 tgagtttgaa aaaggattct cattcacaaa cctagttgac tttgatgccc tttacggcca
     8341 tcgtcgtaat gctcacggtt accgtgattg cttgcatgag tttgatgaac gcttacctga
     8401 aattatcgca gctatgagag agaatgacct tctcttgatt actgcggacc atggaaatga
     8461 cccaacgtat gcaggaacgg atcacactcg ggaatatatt ccattgttgg cctatagccc
     8521 tgcctttaaa ggaaatggtc tcattccagt aggacatttt gcagatattt cagcgactgt
     8581 tgccgataac tttggtgtgg aaactgctat gattggggaa agtttcttag ataaattggt
     8641 ataagatgac gcgctatgct ttgctggtga gaggtatcaa tgttggtagt aagaataagg
     8701 tcgtcatggc ggagcttcat caagaattga caaacttggg actggaaaag gttgagagct
     8761 acatcaacag tggcaatatt ttctttgctt cgatagattc caaagcccaa ttggttgaaa
     8821 agctagagac tttctttgca gtccattatc catttattca gagcttttct ttactgagtc
     8881 tagaggactt tgaggcggaa cttgaaaatc taccagcttg gtggagcaga gacttggcac
     8941 gaaaagattt tctcttttac actgagggtt tggatgtgga ccaagtcatc gcgacagttg
     9001 aaagtttaga gctgaaagat gaagtgcttt attttggaaa acttgggatt ttctggggga
     9061 aattttctga agaatcctat tctaagactg cctatcataa gtacttgctg aaggtgcctt
     9121 tctaccgcca cattactatt cgtaatgcta aaacctttga caaaattggt caaatgctaa
     9181 aaaaataata aaggagacac acaatgacat ttttaaacaa aatccatgaa actgctactt
     9241 tcctgaaaga aaagggaatt gcagcccctg agttcggtct aatccttgga tcaggacttg
     9301 gagaattggc agaagaaatc gaaaatccag ttgtagtaga ctatgctgag attccaaact
     9361 ggggccgttc aacagtagtc ggtcatgctg gtaaattggt atatggtgaa ctggcaggtc
     9421 gcaaggtctt ggctcttcaa gggcgtttcc atttctatga agggaatcct ctggaagtgg
     9481 tgactttccc agttcgtgtg atgaaagttc ttggatgtga aggtgttatt gtaaccaatg
     9541 cagctggcgg tatcggattt ggtcctggta ccttgatggc tatctcagac catatcaaca
     9601 tgacggggca aaatccattg atgggtgaaa acttggatga ctttggccca cgtttcccag
     9661 atatgtctag ggcctacaca ccagaatacc gtgccactgc ccatgaagtg gctaaaaaac
     9721 ttaatatcaa gcttgatgaa ggtgtctata tcggagttac tggtccgact tatgaaacac
     9781 cagcagaaat tcgttcctat aagacactgg gagcagatgc agttggtatg tctacggttc
     9841 ctgaagttat cgtggcagcc cactctggct tgaaagttct gggaatttca tgtatcacta
     9901 actttgcggc cggtttccaa gaagaactca atcacgaaga agttgtagaa gtgactgaac
     9961 gtgttaaagg tgatttcaaa ggcttgctta aagcgattct tgctgaattg taagaaaaaa
    10021 gatttaaaag ggggagtgcc tctgtctttt caggattgac tgcctatccg tattaaagaa
    10081 gaaacagagg aatactatga gcttcttcct gctcttataa ctgaaagaag cggaagaata
    10141 ggtgtgtctg atctgatagc cagcattgtg aaagacaaga ttctaggata ctagcattag
    10201 cttcctagcc aagcagacta gtatgataag gagagatgag aatgaattga ctttctgaat
    10261 ttctcagtct tatcatatat agcacaatga gatttcgctt gagtctgctt gtaaataaac
    10321 gaaaagaaag ataagaaata atgaaaattg gtcaacgaat tatgcgcttt ggcataaaaa
    10381 attaagtatc ggagttgtat ctgttgtagt cggctttgat ttctagctcc agctggaatt
    10441 tcagccaatg aagtaaagca agatgtaaca tctgaagtgg taataggtgt gctagattct
    10501 aaggaggaat tgaaagagtc agaaaatgat gctccaaaac tagaaactcc tcttagagag
    10561 gagccaagac tagctcctca aacgcttccg gaagcaagtg aagttcttga aaacaaaagg
    10621 gaagagtcaa aagtagagat aacagagcca gctcaagcgg atgatatccg caaggttgtt
    10681 ggggaattag ccaaggatat aagtattact aagttgtata tgacaggtca ttctcttgga
    10741 ggctacctag ctcagattgc agcggttgaa gattaccaaa aatatcctga tttttataac
    10801 catgtattga ggaaagtgac aactttcagt gctcctaaag tgattacttc cagaactgtt
    10861 tgggatgcta agaatggttt ctgagatgtt ggtttggaaa gtcgtaaatt agctgttagt
    10921 ggaaaaatta agcattatgt ggttgataat gacaatgttg tgactccctt gattcataat
    10981 aatcgtgata ttgttacatt tacaggtaat tcacgcttta aacaccgttc tcgtggctat
    11041 tttgaaagtc caatgaatga tattcctaac tttaatattg gtaaacaagc taccttggat
    11101 aaacatggtt atcgtgatcc gaaattggat aaagcgcgat tctttaagaa acaggctcta
    11161 cctcaatctt ctagtcaacc aagcgctgaa ccaatggaaa atattgcctt aggaaaacag
    11221 gttactcaaa gttcgacagc tttcggagga gatgctagaa gagctgtgga tggcaaagtc
    11281 gatggtaact atggtcacaa ttctgtcact catacaaact tccaatctaa gccttggtgg
    11341 caagtagatt tggctaaaga agaaaccatt cgccaaatca atatttacaa ccgaacagac
    11401 actgcccagg atagattggc aaactttgat gtcattcttt tagacggttc tggtaaagaa
    11461 attgagtgaa aacgtataat atctcctaaa gatgtgtcag cacaaattac gattaaccat
    11521 aaaaaagcgc gctatgttcg gattgagcta gaaggctata atgccctcag tcttgcagaa
    11581 gttgaagttt tccgctttat agctacgaat ggtgaaacgg cgatacaagt ttctaagtca
    11641 gttcaaccaa tcagtcagac tcctgtgaaa gataaaacat tgacaattca acacagtgga
    11701 gcttacattg cccgctactc cataacttgg gaagaagtta cagtagataa agatggaaac
    11761 caagttgttc gtagtcattc ttgggaagga aacggtcgca accagactgc aggttttgtc
    11821 ctcaacctcc caatcaaaga aaatatgaga aatctgcgag ttaagattga gaaaaagacg
    11881 ggcctactat ggaatagatg gcaaacaatc tatgaaaaca gaccaatttt agctcaaccc
    11941 caccgtaaaa ttacccattg gggtacgaca ttgaattcca aggtgagtga cgatgatgtc
    12001 ttgtaatctg atggtacaat gacagttagt ttgtctagtt tataagaaag tactacctga
    12061 gcttgaatag aactcaggta gctctctgtg aaagaacaaa attaatactc aatgaaaatc
    12121 aaagagcaaa ctaagaaact agccgcaggt tgctcaaagc actgctttga ggttgtagat
    12181 aagactgacg aagtcagtca catatataat ccaaggcgac gttgacgtgg tttgaagaga
    12241 ttttcgaaga gtataaacag aaaggtagag cgcgtgttct aatttgaaca cgagtagaaa
    12301 acttttctaa aaacaaaaac gaaaggatgg gtaaactgta ttcgctgaac tgaatacggg
    12361 cgactctcct ctaaatcaaa attaagaaag gaattgaccc caccctaaaa gtagtgggaa
    12421 aaagatagtt ggtctagcga gcatcgctca ctgcgcccaa ctcctatttt cccttcgctt
    12481 tttgatgggt ttggtatctt tctcaatata aaatataaaa taaagaaagg tagagcgtgt
    12541 gttttgattt gaacacgagc ggaaaactcg gaaaatagat aatctgactg aaaaatcagg
    12601 atttctcgtc aggttcctaa ttttcagtcg ttttcttctc gctctttgta tcataaatta
    12661 tgtctatcca tattgctgct cagcagggtg aaattgctga taaaattctt cttcctgggg
    12721 atcctcttcg tgctaagttt attgcggaga atttccttgg tgatgctgtt tgttttaacg
    12781 aagtgcgtaa catgtttggt tacactggta cttacaaggg tcaccgtgta tctgtcatgg
    12841 gaactgggat gggaatgcca tctatttcga tttatgcgcg tgagttaatc gtagactacg
    12901 gtgtgaagaa attgattcgt gtgggaactg caggttcttt gaatgaagag gttcatgttc
    12961 gtgaattagt tttggcgcag gcggctgcaa ccaactcaaa catcgttcgt aatgactggc
    13021 cacagtacga ttttccacaa attgctagct ttgatttgct tgataaagcc taccatatcg
    13081 ccaaagaact tggtatgact actcacgttg ggaacgtttt gtcatctgat gtcttttact
    13141 caaattactt tgaaaagaat atcgagcttg gtaaatgggg agtcaaggct gtggaaatgg
    13201 aagcagcagc tctttactat cttgctgccc aataccatgt tgatgcgcta gctatcatga
    13261 ccatctctga tagcttggtc aatccagacg aagacacaac tgcagaagaa cgtcaaaata
    13321 ccttcactga tatgatgaag gttggtttgg aaaccttgat tgcagaataa ttatagccaa
    13381 aaaggaagcc ctttatgaat tgtaggactt ccttttctta tccagaaatt gatctagctc
    13441 tctctgattt cgaagaatag tgactttatg tgaatattct tggcaaagtt tttggtaatt
    13501 ttctttttga gttttgctac gcccatccca aagaatccat ctgataaact cccagtcaaa
    13561 gcgttcaggg caatctgccg ccatactttc tctgactttt ccacggtatt taagataacg
    13621 cttaaaggct ctaaagagac aggtcaatgg caaaaaattg agaaagatga tttggtcagc
    13681 ttcttgcatt cgttcttggt agtagcacca agaataatta ccatcgatga cccaagcttt
    13741 atgcttggtg agaaagtttt ttatctcggt taacatccat tcgcagtcac tgtcttgcca
    13801 accaggttga aattggagtg tgtccatgtg cagttttgga atggagtagt agttagataa
    13861 cttttctgct agagttgact taccagaacc agaatatccg ataattgtga ttttcatttt
    13921 ctaccttttc ctatttggag acaaaaaaac agcctctatg gactgtttct tatttagcaa
    13981 gtttagttga aagacgagct ttatcgcggc ttgctttgtt tttgtgaatc aaacctttag
    14041 tttctgcttt atcgatagct gagctagcag cacggaaaag ttcttcagat gggtttgctt
    14101 cgaaagcttt tatagcagta cgcatagctg atttttgagc tgagttcttt tcgttttgtt
    14161 taacgttcaa ttcagcgcgt ttgatagctg atttaatgtt tgccaatgtt cttacctcca
    14221 tatttactaa ctataccatt atatatgaaa acttacgttt tgacaaggga aaattacttt
    14281 tttaagtaaa tttcatcgat ttcatggttc ttgcttttat gaagaatcac ttctgcacga
    14341 tttctggttg gttcaatata attttgcaga tttgtgagat tgatactgat ccagacctga
    14401 tgggcaaagg actccacttc cccaatcggc atctgagtaa aacgataata gtagctatca
    14461 gggtcgtttt gggctaggct cagcatcttc aagaaacggt ccagatacca actttcaata
    14521 tcatccactc cagcatctac atagatggaa aagtcaaaga agtcagtgat atagagacga
    14581 tcgttttgtg gattttgaaa gacattaatt ccctcaacaa ttacaaaatc agcagctttg
    14641 acactttgtt tcttttcggg tacgatgtcg taaacttcat gagaatagac aggaatatct
    14701 acatcttgtc catttttgat gcggtccaag aagttgagaa gagcttccat atcatagctt
    14761 tcaggaaatc ctttacgatt taaaatcccc tgctcaatca aggtttgatt gggatagaga
    14821 aaaccatcag ttgtaaccaa ctcaaccgta gcatctgtaa acgtacggga cagtaggatt
    14881 tgaagtaggc gactggttgt ggattttcca acggcaacac tcccagaaac cccaataata
    14941 aaaggttgag atttactttc acgttggagg aaaattcctt ttgaaaaggc taaatcttcc
    15001 ttagttcgct tgtaaatctg aatcagatga gccaagggga gatagatatc tgtaacgtct
    15061 tggagactga tttggtcatt aaaactcttg atagattcca attcttcttc tgtcaaagga
    15121 ggtgttgtct ttcgatgtaa agattgccaa gtctggcggc tgattttttc aaaatgtaaa
    15181 aattcgttgg tcatttgttt tcccctagat attttgtcta tcataccata aaaagctaaa
    15241 aaaataaagc ggtttcttga atgtggtaag tcaatggtat atagtataag tgatcaattt
    15301 attgatgaaa taggagatag attctactga tttataagct tataaagggg gctttttagt
    15361 tcacaactta taggaggtgt actatgaaaa tcttaaaacg ttacatattg gaactctgtt
    15421 ttattttaag ttttgcttta ccttttataa aaggaaccaa tgcagataat ggtagatgct
    15481 ttgtggaaac ctattacggt tttacttttt tgatggaaca tgctattgta acagctgtct
    15541 ttatctgttc gttcttaatt gctttcttac taaaaaacga tggacgaaat ggattgctgc
    15601 gggtagttat tgctttttag tcctacggat tgcgacagag ggttatttct tccacatgtc
    15661 actagaagat ttgatacgac tttggacaag tttggaaatc ctgacacaaa cgtatcagtt
    15721 gggcttttat ctaaacattt tgttaggaat tttgttgata ataaagtatt ttaaggttaa
    15781 gcaatagaaa taataatctg cctgattata gatgataaac tttttgtttt tatctggact
    15841 gtgatattta tagttctaaa tggtttatta tttataagtg ttgaaagaga ttgaagaaaa
    15901 tggcttcgtg acttgctttc ttcggtcttt ttcgttttat tatcaatttg taaacgattt
    15961 acaatttgag tcaaattatg gtaaaatagt gtcatgagta aaatgtacta tgcagaaaat
    16021 cctgacgctg ctcacgacat tcatgagttg agagtggact tgttgggaga aaaaatgact
    16081 tttttgacag atgcgggtgt ttttagcaag aaaatggttg actttggaag tcaactcttg
    16141 ctcaagtgtc tggatgtcaa ccaaggagag acagtcctag atgtaggctg tggttatggt
    16201 ccattaggtt tgtccttggt caaggtttac ggagttcagg cgaccatggt tgatattaac
    16261 actcgtgccc tggatttagc tcagagaaat gctgaaaaaa ataatgcaaa agcgacgata
    16321 tttcaatcta atatttatgc aagagttcaa gggcgatttg accatgtcat ttccaatcct
    16381 cctatccgtg cgggcaagca agtcgttcat gaaatcatcg agaaaagtaa agatttcttg
    16441 gaaattggtg gagatttgac catcgtcatc cagaaaaaac aaggagctcc aagtgccaaa
    16501 tctaagatgg aagatgtgtt tggcaattgt gaaatcctta aaaaagacaa gggatactat
    16561 atccttagaa gtgtgaaaga atgagagcag ttgatttaat ccaaaaaaaa acgagacggt
    16621 caagaactga cttcaagtga aattgaatgg ctgatagaag gctatgtatc aggaactgtt
    16681 cctgattatc agatgtctgc ctttgctatg gctgtttatt ttaaaggaat gacgactcga
    16741 gaaatctctg atttaacgat gaatatggtt aagactgggc aagagtttga cctgtcagcc
    16801 attgatggtg tgaaagttga caagcattct actggtggtg tcggcgataa ggtaaccttg
    16861 attttagctc ctcttgttgc tagcttcggt gtgcctgtag caaaaatgag tggtcgtggt
    16921 cttggccata caggcggaac aattgataaa ttggagtcca ttaggggcta tcaagtagaa
    16981 cgtagtcaag aggattttat tcgtcaggtt caggatattg gagtatctgt cattgggcaa
    17041 tcagaccagc tggtcaaagc agataagctt ctctatgccc ttcgtgatgt gaccgcaact
    17101 gtcgacacga ttcctttgat tgcgagttcg gtgatgagca agaaaattgc ggcaggggcg
    17161 gatgctattt tgctagacgt gactgtcggt gagggtgcct tcatgaagac ggttgatgag
    17221 gcgcgtgagt tggctcaaac tatggtagat cttggtaagg ttgttggacg gaagacagta
    17281 gcagtgatta cggatatgag ccagcctttg ggacgagcga ttggaaatcg tctggaaatc
    17341 cttgaagcat tggagatttt gcaaggtcaa ggacgtcagg atattaccca ctttatttgt
    17401 gaattggctc aaattatgct tggtttggca aatgttaaca aaactgttga agaagttcgc
    17461 caacatcttg aaaatggtca agcactggct aagtttgagg aaatggtgca agcccaaggt
    17521 ggagacttgg aagacctcta tcgtcctgta aatgtagctc atgtggtgga aatccctgct
    17581 caggaaacgg gtgtcatttc agctcttcca gctatggact ttggtcttta tgccatgagg
    17641 ctgggtgctg gtcgtgcagt taagtctgat gtcttggact atgaaacagg aattgttttt
    17701 gaaaagaaga ttggagactc cgttcaaaag ggtgaaattg ttgcaaaagt atacacaaat
    17761 gaaaaaattc cacctcaact agttacagat ttccaaaaat gtgttaaaat aagtgatgaa
    17821 gtgaaaaaaa tccgtgaaat tgtagaaatc atctcctaaa tggagggaaa accgaaatga
    17881 aattaaataa atatatagat catacgcttt taaaacaaga tgcaaagaaa aaacaaattg
    17941 atagtttgtt gtctgaggct agagagtatg gctttgccag tgtttgcgtt aatccgacct
    18001 gggttgaaca tgctaaaaaa ggacttgaag gcacagatgt taaggtttgc acagtagtag
    18061 gtttcccctt gggagcaaca acttcaactg tgaaagcttt tgaaacaaaa gaagctatcc
    18121 aaaatggggc tgatgagatt gacatggtaa tcaatgttgg ggctctcaag tcaggtgatt
    18181 tggccttggt tgagtcggat attcgtgcag tagtggaagc aagtggggac aagttagtga
    18241 aagttattat tgaagcttgc cttctgacag accaagaaaa agttttggct tgccaattag
    18301 cccaaaaagc aggagctgac tttgtcaaaa catctactgg cttttcaact ggtggtgcta
    18361 cgatagcaga tgttagatta atgcgtgaaa cagttggacc tgatatgggt gtcaaggccg
    18421 ccggtggagc tcgttcttat gcagatgctc ttacctttgt cgaagcaggt gcgacccgta
    18481 tcggaacgtc agctggggta gctattttaa aaggagaatt ggcagatggc gactactgag
    18541 ttgattgaac tggcaattga aaccagcaaa catgcctatg tcccctattc tcactttcct
    18601 atcggagcag ttttagtagc caaagacgga agtgtttaca cgggagtgaa tatcgaaaat
    18661 gctagctatc ctttgaccaa ttgcggtgag agaacagcta tttttaaggc tatatctgaa
    18721 ggccaaagag aattttcaga attgattgtc tatggtcaaa ctgaaaaacc gatttcacca
    18781 tgtggtgctt gtcgccaagt aatggtcgaa ttttttgaac aagatttaaa agtgacccta
    18841 gtcgcaaaag ataaatcgac ggtcgagatg acggtcgggg agttacttcc atactctttt
    18901 acagacttaa attagtctga gtcgctcttt gagtggcaag ggtctttgtg accaatcaat
    18961 ccatacttgc aacatcgttg cacatcttat ttaggaggtt cagtaatgaa caagaaacaa
    19021 tggctaggtc ttggcctagt tgcagtggca gcagttggac ttgctgcatg tggtaaccgc
    19081 tcttctcgta acgcagcttc atcttctgat gtgaagacaa aagcagcaat cgtcactgat
    19141 actggtggtg ttgatgacaa atcattcaac caatcagctt gggaaggttt gcaagactgg
    19201 ggtaaagaac acaatctttc aaaagataac ggtttcactt acttccaatc aacaagtgaa
    19261 gctgactacg ctaacaactt gcaacaagcg gctggaagtt acaacctaat ctttggtgtt
    19321 ggttttgccc ttcacaatgc agttgaagaa gcagctaaag aacacactga cttgaactat
    19381 gtcttgattg atgatgtgat taaagaccaa aaaaatgttg cgagcgtaac tttcgctgat
    19441 aatgagtcag gttaccttgc aggtgtggct gcagcaaaaa caactaagac aaaacaagtt
    19501 ggttttgtag gtggtatcga atctgaagtt atctctcgtt ttgaggcagg attcaaggct
    19561 ggtgttgcgt cagtagaccc atctatcaaa gtacaagttg actacgctgg ttcatttggt
    19621 gatgcggcta aaggtaaaac aattgcagcc gcacaatacg cagccggtgc agatattgtt
    19681 taccaagtag ctggtggtac aggtgcaggt gtctttgcag aggcaaaatc tctcaacgaa
    19741 agccgtcctg aaaatgaaaa agtttgggtt atcggtgttg accgtgacca agaagcagaa
    19801 ggtaaataca cttctaaaga tggcaaagaa tcaaactttg ttcttgtatc tactttgaaa
    19861 caagttggta caactgtaaa agatatttct aacaaggcag aaaaaggaga attccctggc
    19921 ggtcaagtga tcgtttactc attgaaggat aaaggggttg acttggcagt aacaaacctt
    19981 tcagaagaag gtaaaaaagc tgtcgaagat gcaaaagcta aaatccttga tggaagcgta
    20041 aaagttcctg aaaaataatg gatggaagtc atttcttagg aagcggccaa gggccgcttt
    20101 tttaagagtt gagacaaagt cattttgtct cagtaaagct tgctactaga caaactagca
    20161 agttttattg aaataaaata agactctgaa aggaagagca catggcacac gaaaatgtca
    20221 ttgagatgcg tgatattacc aaggtgtttg gtggatttgt tgccaacgac aaaatcaact
    20281 tgcacctacg aaaaggtgaa attcatgcac ttttaggaga aaatggggct ggtaagtcca
    20341 cgctaatgaa catgttagca ggccttcttg aaccaaccag tggtgaaatc gcggtcaacg
    20401 gtcaagttgt caatctcgac tccccatcta aagcagctag cttgggaatc gggatggttc
    20461 accagcactt tatgttggtt gaagccttca cagtggctga aaacatcatt ttaggtagtg
    20521 aattgactaa aaatggtgtg ctagatatcg ctggagctag caaagaaatc aaggctcttt
    20581 ctgaacgtta tggcttagct gttgaccctt ctgccaaggt agcagatatc tcagttggag
    20641 cccaacaacg tgtagaaatt ttaaaaacac tttatcgggg ggctgatatc cttatctttg
    20701 acgaaccaac ggctgttttg actccatcag aaattgatga gttgatggct attatgaaaa
    20761 atcttgtcaa agaaggaaaa tcaattatct tgattaccca caaattggat gaaattcgcg
    20821 cagtttctga ccgtgttaca gttatccgtc gtgggaaatc aattgaaacc gttgaaattg
    20881 caggggctac caatgctgat ttggcggaaa tgatggtagg acgttctgtt tcctttaaaa
    20941 cagagaagca agcctctaaa ccaaaagaag tggttttgtc tatcaaagat ttggtggtca
    21001 atgaaaaccg tggtgttcca gctgttaaaa atctgtcctt ggatgttcgt gctggagaga
    21061 ttgttggtat tgcggggatt gatggaaatg gtcagtctga actgattcaa gccattacag
    21121 gtcttcgtaa ggttgaatct ggtagcattg agctaaaagg agattcaatt gtaggcttgc
    21181 acccacgtca gattacagaa ctaagtgttg ggcacgttcc agaagaccgt caccgtgatg
    21241 gcttgatttt ggaaatgatg atatctgaaa atattgccct tcaaacctac tataaagaac
    21301 cacatagtaa aaatggaatt ttgaattatt caaatattac ttcttatgct aaaaagctga
    21361 tggaagagtt tgatgttcgc gctgccagtg aattagttcc tgcagctgca ctctcaggag
    21421 gaaatcaaca aaaagcaatt attgctcgtg aaattgatcg agatcctgat ctccttatcg
    21481 ttagccagcc aactcgtggg ttggatgtcg gtgccattga gtatatccac aaacgcttga
    21541 ttgaagagcg tgataatggc aaggctgtcc ttgttgtcag ctttgaattg gatgagattt
    21601 taaacgtctc agaccgtatt gccgttatcc acgatggtaa gattcaaggt attgtatcac
    21661 cagaaacaac caataaacaa ggacttggtg tcttgatggc tggtggaaac ttgggaaagg
    21721 agaagagtga tgtctaaaaa attacaacaa atttcggttc ccttgatttc tgtattccta
    21781 ggaattttac tcggagccat tgtcatgtgg atcttcggtt atgatgctat ttggggctac
    21841 gaagaattgt tctatacagc ctttggcagt ctgcgtggga ttggagaaat cttccgtgct
    21901 atgggtcctc tggtcttgat tggttttggt tttgcggttg ccagtcgagc tggtttcttt
    21961 aacgtcggac ttcctggtca ggctttggca ggttggattc tcagtggttg gtttgccctg
    22021 tcgcatccag atatgccccg tcccttgatg attctagcaa ccatcgtgat tgccttgatt
    22081 gctggtggga ttgtcggagc gattccaggt attcttaggg cctatctagg gacgtcagag
    22141 gttattgtaa ccatcatgat gaactacatt gtcttgtatg tagggaatgc ctttatccat
    22201 gctttcccta aagacttcat gcaaagtaca gattcgacca ttcgtgttgg ggctaatgca
    22261 acctatcaga caccttggtt agctgagttg actggtaact cacggatgaa tattggtatt
    22321 ttctttgcca tcattgccgt tgcagttatt tggttcatgc tcaagaaaac aactcttggt
    22381 tttgaaatcc gtgcagttgg tcttaatcca catgcttcag aatatgctgg tatttctgcc
    22441 aagcggacta ttatcctatc tatgattatt tcaggtgcct tggcaggtct tggtggagct
    22501 gttgaaggtt tgggaacctt ccagaacgtc tatgttcaag gttcgtcatt agctatcgga
    22561 tttaacggaa tggcggttag tttgcttgcg gccaactcac caattggtat actctttgca
    22621 gccttcctat ttggcgttct ccaagttggg gctcctggta tgaatgcggc gcaggtacca
    22681 tctgagcttg tcagcattgt aacagcgtct attatcttct ttgtcagtgt tcattacctt
    22741 atcgaacgct ttgtcaaacc gaaaaaacaa gttaaaggag gtaagtaaag atgtctatta
    22801 taaccttgct ccccctcttg gtgtcttcta tgctgattta ctcagcacct ctcatcttta
    22861 caagtatcgg tggtgttttc tctgaacgtg gtggtgtggt aaacgttggc cttgaaggaa
    22921 ttatggttat gggtgccttt tctggagttg tctttaacct tgaatttgca gaacaatttg
    22981 gagcagcaac tccatggcta tccttgcttg tagcaggatt ggttggtagt gttttctcta
    23041 tcatccacgc agcagcgacg gttcatttcc gtgcagacca tgttgtcagc ggtacggtat
    23101 tgaacttgat ggcgcctgcc ttggctgttt tcttagttaa agttctttat aacaaaggac
    23161 aaaccgacaa cctaagtcaa acttttggac gctttgattt cccagtcttg gcaaatatcc
    23221 cagtgattgg tgatatcttc ttcaagtcaa ctagtctact tggttatctg gcgattgcct
    23281 tctcattcct tgcttggttt attctcttta agactcaatt tggtcttcgt ctccgctctg
    23341 tcggtgaaca tcctcaagca gcggatacct tgggaatcaa cgtctacaag atgagatatt
    23401 taggggttat tatttcaggt tttctaggtg gaattggcgg agcgatttat gcgcaatcaa
    23461 tctcagttaa cttctcagtg acaactattg ttggacctgg atttatcgcc cttgctgcga
    23521 tgatctttgg gaaatggaat ccaatcggtg ctatgctttc tagtctcttc tttggacttt
    23581 cacaaagttt ggctgttatc ggttctcaat tgccgttcct acaaggagtg ccagcggttt
    23641 atcttcaaat tgcaccttat gttttgacaa ttcttgtctt agcagccttc tttggaaaag
    23701 cagtcgcacc aaaagcagat ggtatcaact acatcaaatc aaaataagca tacaaaaaaa
    23761 cgtcagtcca atgactgaac tgccccccaa aagttagaca gaaaaaatct aacttttggg
    23821 gtgtttttat tatgaaatta acttatgatg ataaagttca gatctatgaa cttagaaaac
    23881 aaggatatag cttagagaag ctttcaaata aatttgggat aaataattct aatcttagat
    23941 atatgattaa attgattgat cgttacggaa tagagttcgt caaaaaagga aaaaatcgtt
    24001 actattctcc tgatttaaaa caagaaatga ttcataaagt ctgacatgaa ggctggacta
    24061 aagacagagt ttctcttgaa tacggtctcc caagtcgtac gatacttctt aactggctag
    24121 cacaatacag aaaaaaacgg gtatactatt gttgagaaaa caagagggag agtacctaaa
    24181 atgggacgta agcccaaaaa gagacctgaa gagaggacag aattagaaca tcttcaagca
    24241 gaaaatgagt acctgagagc ggagaatgcc atcctaaaaa agttaagaga actccgattg
    24301 aaggaggaaa aagagaaaga agaaagacag aaattgttca agaattaatg actgagtttt
    24361 cgttagatat tcttctaaaa gccattaaac tagctcgttc gacctactac tatcacttga
    24421 aacagctaga caaaccagat aaggaccaag agcttaaagc tgaaattcaa tccattttta
    24481 tcgaacacaa gggaaattat gcttatcgtc ggatttattt agaactaaga aatcgtggtt
    24541 atctggtaaa tcataaaaga gttcaaggct tgatgaaagt actcaattta caagctaaaa
    24601 tgcgacagaa acgaaaatat tcttctcata aaggagacgt tggtaagaaa gcagagaatc
    24661 tcattcaagg ccaatttgaa ggctctaaaa caatggaaca gtgctacaca gatgtgactg
    24721 aatttgccat tccagtaagt actcaaaagc tttacttatc accagtttta gatggtttta
    24781 acagcgaaat tatcgcctat aatctttcaa cttcacccaa cttagaacaa gtacaaacaa
    24841 tgttggaaca ggcattcaca gagaagcact acgagaatac gattctccat agtgaccaag
    24901 gctggcaata tcaacacgat tcttatcatc agtttcttga gggtaaggga attcaagcat
    24961 ccatgtcacg taagggtaac agcccagaca atggtatgat ggagtccttc tttgggattc
    25021 tgaaatcgga aatgttttac ggttatgaga agtcatttca gtcgcttaag caattggaac
    25081 aagccattgt agactatatt gattactaca acaataaacg aattaaggta aaactaaaag
    25141 gacttagccc tgtgcaatac agaactaaat ccttcggata aattaattgt ctaacttttt
    25201 ggggtcagta cagaactggc gttttatttt ttaggatctt gatgggttag gttcaatccc
    25261 caagggacca aattttcagt tttatttttg atacgagcta tattgtcctt atgacgaatg
    25321 ataatcaaac tagcaagtgc taagataata gcgatgaaga gagagtcata gttactcagg
    25381 ataaaaccaa aaagtggaaa gagcagaacc ccgataacag ccgcgatcga tgctgtgaca
    25441 ctagacagtg aaatcatact gccaagatag agagctccaa agaagataat cgcaaggtag
    25501 agacagaaga taggcgcaaa tccgaaaatc actccagcac tggttgcgac agccttacca
    25561 cctttaaatc ctgcaaatat agggaaggta tggccgataa cagccaaaag tccaaagatg
    25621 agaggagaaa cgccttgtag atgaaaaata atcggaagca gcgttgctag ggttcctttg
    25681 aaaaagtcaa tcacaaaggt tgccatacca gctttcttac ctaaaatgcg gaaggtgttg
    25741 gtcgttccag tgttaccaga accatgctcg cgtagattga tttgaaagaa tacttgtcca
    25801 atccagagac cagatggaat cgaacccagc agataggcta ggattaataa aactattgta
    25861 atcatactcc tattatatca tgaattggga aagaaaagca gagaatctct gctgaaattg
    25921 tcacatcgga gaaagaaaaa ttttgcaaaa tccttggaaa acctgtagaa tagtaaagat
    25981 gaacgaatag gaggttcctt gtgtcaaaaa aggaaatcaa tattaacaat tataatgatg
    26041 atgctattca ggtgctagaa gggttggatg cggtccgaaa acgtccaggg atgtatattg
    26101 gatcgaccga tggcgctggt cttcatcacc tagtttggga aatcgttgat aatgcagtcg
    26161 atgaagcctt gtctgggttt ggtgatcgta ttgatgtaac tatcaataaa gacggtagtc
    26221 taacggttca agaccatgga cgtgggatgc cgacaggtat gcacgctatg ggaattccaa
    26281 ctgttgaggt tatctttacc attcttcatg ccggagggaa attcggtcaa ggtggctata
    26341 agacatcagg tggacttcac ggagtgggtt cttccgttgt taacgccctt tctagctggt
    26401 tagaagttga aattacccgt gatggcgcag tttacaagca acgttttgaa aatggtggaa
    26461 aacctgtcac gactttgaag aaaatcggta cagcacccaa gtctaaaaca ggcaccaaag
    26521 ttacttttat gcctgacgcg actatctttt ctacgacaga tttcaagtac aataccattt
    26581 cagagcgcct taatgaatca gcctttctct tgaaaaatgt gaccttgtct ttaacggaca
    26641 agcgaacaga tgaagcgatt gagttccact atgagaatgg agtacaagat tttgtttctt
    26701 atctcaacga agataaggaa atcttgacgc cagttcttta ctttgaaggg gaagacaatg
    26761 gttttcaagt ggaagtagcc ctccagtaca atgacggatt ctcagataac attctatcct
    26821 ttgtcaataa cgttcgcacc aaggacggtg gaacgcacga gacaggactc aagtctgcca
    26881 ttaccaaggt catgaatgac tatgcacgta aaacaggtct tctcaaggaa aaagataaaa
    26941 accttgaagg ttcagactat cgtgagggac tagcggccgt tctttctatc ttagttcctg
    27001 aagaacactt gcagtttgaa ggacagacca aggataaact aggaagcccc ctagctcgcc
    27061 cagttgtgga tggaatagtg gctgataagt tgaccttttt ccttatggaa aatggggaat
    27121 tagcttctaa cctcatccgc aaggctatca aggcccgtga tgctcgtgaa gcagcacgta
    27181 aggcgcgtga tgagagccga aatgggaaga aaaacaagaa agataagggc ttgttgtctg
    27241 ggaaattgac cccagcccaa tctaagaatc ctgctaagaa tgaactctat ctagttgagg
    27301 gggactctgc cggtggttct gccaaacaag gtcgtgaccg caagttccag gctattctac
    27361 ctcttcgtgg taaggttgtc aatacagcca aggccaagat ggcggatatc ctcaaaaatg
    27421 aagagatcaa taccatgatt tataccattg gtgcgggtgt tggagcagac ttctctattg
    27481 aagatgccaa ctatgataag atcattatca tgaccgatgc ggataccgac ggtgcccata
    27541 tccagacctt gctcttgaca tttttctacc gttacatgcg tccgctagtc gaggcaggtc
    27601 atgtctatat tgccctccca cctctttaca agatgtccaa aggtaaaggc aagaaagaag
    27661 aagtggccta cgcttggacg gacggagaac tagaagaact ccgtaaacag ttcggtaaag
    27721 gcgctaccct ccaacgatac aaaggacttg gtgagatgaa tgcggaccag ctctgggaaa
    27781 caaccatgaa cccagaaaca cgtaccctca tccgtgtcac aattgaagat ttagcgcgcg
    27841 ccgaacgccg cgtcaatgtt ctcatgggag ataaggtaga accacgccgt aaatggattg
    27901 aagataatgt caagtttacg ctagaagaag cgacagtgtt ttaatgaaag aaaataatat
    27961 gttagtaaac tggaaattag agtcggatgt gaatgactat gtgaaaaagc agtttgaaaa
    28021 tcttggtttg aaaaaattgc aggattatta atgaagaatc tgctatgagt gcttatctca
    28081 aagaggcgtt taagggagcg gctaagacac aaactaagac taattttgga aaaccatatt
    28141 ttcatattga aaaatacaaa caggatagtt tggttactta tcgtaactga acacgggacg
    28201 aattcccgta aaatatcttt tactgcataa aaacctactc tacattcttt gaaaggagtt
    28261 gaacacgccc tagatactgt gtgaaaaaga taaattctct tgtgagtttg cttacttcaa
    28321 gaattttcta ttttcacttg gtattttatg ggctttgtat cttatgtcta acattcaaaa
    28381 catgtccctg gaggacatca tgggagagcg ctttggtcgc tactccaagt acattattca
    28441 agaccgggct ttgccagata ttcgtgatgg gttgaagccg gttcagcgcc gtattcttta
    28501 ttctatgaat aaggatagca atacttttga caagagctac cgtaagtcgg ccaagtcagt
    28561 cgggaacatc atggggaatt tccacccaca cggggattct tctatctatg acgccatggt
    28621 tcgtatgtca cagaactgga aaaatcgtga gattctagtt gaaatgcacg gtaataacgg
    28681 ttctatggac ggagatcctc ctgcggctat gcgttatact gaggcacgtt tgtctgaaat
    28741 tgcaggctac cttcttcagg atatcgagaa aaagacagtt ccttttgcat ggaactttga
    28801 cgatacggag aaagaaccaa cggtcttgcc agcagccttt ccaaacctct tggtcaatgg
    28861 ttcgactggg atttcggctg gttatgccac agacattcct ccccataatt tagctgaggt
    28921 catagatgct gcagtttaca tgattgacca cccaactgca aagattgata aactcatgga
    28981 attcttgcct ggaccagact tccctacagg ggctattatt cagggtcgtg atgaaatcaa
    29041 gaaagcttat gagactggga aagggcgcgt ggttgttcgt tccaagactg aaattgaaaa
    29101 gctaaaaggt ggtaaggaac aaatcgttat tactgagatt ccttatgaaa tcaataaggc
    29161 caatctagtc aagaaaatcg atgatgttcg tgttaataac aaggtagctg ggattgctga
    29221 ggttcgtgat gagtctgacc gtgatggtct tcgtatcgct atcgaactta agaaagacgc
    29281 taatactgag cttgttctca actacttatt taagtacacc gacctacaaa tcaactacaa
    29341 ctttaatatg gtggcgattg acaatttcac acctcgtcag gttgggattg ttccaatcct
    29401 gtctagctac atcgctcacc gtcgagaagt gattttggcg cgttcacgct ttgacaaaga
    29461 aaaggctgag aaacgtctcc atatcgtcga aggtttgatt cgtgtgattt cgattttgga
    29521 tgaagtcatt gctcttatcc gtgcttctga gaataaggcg gacgccaagg aaaacctcaa
    29581 agttagctat gattttacgg aagaacaggc tgaggctatc gtaactttgc aactgtaccg
    29641 tttgaccaat accgatgtgg ttgtcttgca ggaagaagaa gcagagcttc gtgagaagat
    29701 tgctatgctg gtggctatta tcggtgatga aaggactatg tacaatctca tgaagaaaga
    29761 acttcgtgag gtcaagaaga aatttgcaac tcctcgtttg agttctttag aagacactgc
    29821 gaaagcaatt gagattgata cagctagtct tatcgctgag gaagatacct acgtcagcgt
    29881 gaccaaggca ggttacatca agcgtaccag tccacgttcc tttgcggctt ccaccttgga
    29941 agaaattggc aagcgtgatg atgaccgttt gatttttgtt caatctgcca agacaaccca
    30001 gcacctcttg atgttcacaa gtcttggaaa tgtcatctac agaccaatcc atgagttggc
    30061 agatattcgt tggaaggaca tcggagagca tctgagccaa accatcacaa actttgaaac
    30121 gaatgaagca atcctttatg tggaagtact ggatcagttt gacgatgcga caacctactt
    30181 tgcagcgact cgccttggtc aaatcaaacg ggtagagcga aaagaattca ctccatggcg
    30241 gacctataga tctaagtctg tcaagtatgc taagctcaaa gacgatacag atcagattgt
    30301 agcagtggct ccgattaaac tagatgatgt tgtcttggtt agtcaaaatg gttatgccct
    30361 gcgtttcaat atcgaagagg ttccggttgt cggtgctaag gcagcaggtg tcaaggctat
    30421 gaatttgaaa gaagatgatg tcctccaatc tggctttatc tgtaatactt cgtccttcta
    30481 cctcttgacc cagcgtggaa gcttgaaacg tgtttccatt gaggaaattc tagcaaccag
    30541 ccgtgccaaa cgaggattac aagtcttgcg tgagttgaaa aacaaaccgc atcgtgtctt
    30601 cttggcagga gcagttgcag agcaaggatt tgttggcgat ttcttcagta cggaagtgga
    30661 tgtgaacgac caaactctgc ttgtccaatc caataaagga acaatctatg aaagccgatt
    30721 gcaagacttg aacttgtcag aacgcactag caatggaagc ttcatttctg acacgatttc
    30781 agatgaagaa gtttttgacg cttatcttca ggaagtagtt actgaagata aataagtgaa
    30841 atgaagaatc agttctaaga gctggttttt attggagaaa ttttctgaaa attacaaaat
    30901 atacttgcta ttttagaaaa atagtgtaga atataagaaa taggttttta gaataaggag
    30961 tggaatatga cagtaacgat tgattgggaa aacctcggtt tttcctatat gaaattacct
    31021 tatcgctatc ttgctcattt caaaaatgga caatgggatc aaggagagct tacagaggat
    31081 gcaactttgc atatttcaga gtcttctcca agtcttcact atggacaaca agcatttgaa
    31141 ggtttgaaag cttatcgtac taaggatggc agtgttcaac tgttccgtcc tgatgaaaat
    31201 gctaaacgcc tgcaacgtac atgtgaccgt ctcttgatgc cacaagttcc gacagacatg
    31261 tttgtagaag cttgtaaagc agttgtccgt gcgaatgaag aatacgtacc accatacgga
    31321 ataggtggaa ccttatatct tcgccctctt ttgattggtg tcggagatat tatcggggta
    31381 aaaccggcag aagaatacat tttcaccatc tttgctatgc cagttggaaa ttactttaaa
    31441 ggtggtttgg tcccaaccaa cttcttgatt caggatgagt acgaccgtgc agcaccaaat
    31501 ggtacaggtg cggctaaggt tggtggaaac tatgctgcaa gtctcttacc aggaaaaatg
    31561 gccaagtcac gtcatttctc agatgttatc tatctggacc catcaactca tacaaagatt
    31621 gaagaagtcg gatcagctaa cttctttgga attacagctg ataatgaatt tgtaacacca
    31681 ttgagtccat ctatcttgcc atctattacc aagtattcct tgctttattt ggcagaacat
    31741 cgcttgggat taactcctat tgagggtgat gttccaattg ataatcttga ccgttttgta
    31801 gaggcaggtg cctgtggtac agcagcggtt atttctccaa ttggaggtat tcaacatggt
    31861 gatgatttcc atgtattcta tagtgaaaca gaagtaggtc ctgtgacgcg taaattatat
    31921 aatgaattga cgggtattca gtttggcgat attgaagcgc cagaaggttg gattgtaaaa
    31981 gtagattaaa ataaaccaaa ggagattttt tatgaaatag aaaaagcggc tcttaacagc
    32041 aggagtggtc ctgagcacgt cagctatttt agtggcttgt ggaaaaactg ataaagaagc
    32101 agatgcaccg acaacatttt cttatgtcta tgcagtagat ccagcatcat tgggctacag
    32161 tatagcgact cgaacatcga cgacagacgt tattggaaat gttattgatg gtttgatgga
    32221 aaatgataaa tacggcaatg ttgctccttc tcaaaaagac tatgatttga atagtacagg
    32281 atgggctcca agctatcaag atccagcgtc ttacttgaat attatggatc caaaatctgg
    32341 ttctgccatg aaacaccttg gcattacgaa aggaaaagat aaggatgttg tagctaaacc
    32401 tggtttggat aaatataaga aattgttaga agatgctgtt tctgagacca ctgacctaga
    32461 gaagagatat gaaaaatatg ccaaagctca agcttggtcg acagatagtt cattattgat
    32521 gccaacagct tcatctggtg gttctccagt tgtaagtaac gtagtaccat tctcaaaacc
    32581 atactcacaa gttggtatta agggggaacc atatatcttt aaaggaatga aattgcaaaa
    32641 agatattgtt acaacaaaag aatataacga ggtttttaaa aaatggcaaa aagaaaaatt
    32701 ggaatccaat agcaaatacc aaaaagaact agaaaaatac attaaataag gaatggtatt
    32761 gatcttgata aaatttttaa aatactgtca ttttgaatat aaaggagttt gatatggagt
    32821 ggattagatt aataggaata gcaatcattg ttgtgggttt tattttaaaa tttgatacaa
    32881 ttgcaacagt agtcttagct ggtttggtta cagctttagt ttcaggtgtt tctctcgttg
    32941 aatttttgga gattttggga aaagaattta gcaatcagcg agtgctcacg atttttatgg
    33001 ttaccttgcc tcttgtgggg ctgtcagaaa cctttggact caagcaaaga tcaatcgatt
    33061 tgattcgaaa gattaaaggt ctgacagttg gaaacttcta tacagtttat ttctttattc
    33121 gagagttagc tggtttcttt tcaattcgtc taggaggaca ccctcagttt gtcagacctt
    33181 tggttcaacc tatgggagaa gcagctgcag agtctcaatt aggtagaaag ttaacagagg
    33241 ttgaagatga gacaataaaa gcgcgtgcgg ctgcgaatga aaattttgga aatttctttg
    33301 ctcaaaatac gtttgtaggt gctgggggag tcctcttgat agggggaaca ttagatcagt
    33361 taggctatga aagcaattat gcagggattg cttctacatc tattattgtt gctgttataa
    33421 cacttattgt agtggggatt tacaattatt tatttgataa aaaattgata tcaaaaaaga
    33481 ctaggggagg agaacaaaaa tgacagagtt ggcaaagcaa ctattagagt tgacctatat
    33541 tgtgattggt tgtcaatttc tccatacagc ctattgtagt tataaagata aaacaaaccc
    33601 agttcgactt gggacatctg cattttggac tctattgtct attacgttta taggtggttc
    33661 ctatatgcca aatatgagta ttggtattat tgtaatccta ttatcgctgt taacattgtt
    33721 taagcaagtc cgtatcggaa ccttgccatc cttagatgaa atgaaagcca atattgaatc
    33781 taacaggttg aaaaataaaa tttttattcc agttatgctg atggcaatac ttgcgttggt
    33841 cttagcgcaa atgattccag aatttagcaa gatttcgatt agccttgccg ccttgtttgc
    33901 tacaatttct gttcttgtga ttaccaatag tcaccctaag agtctgttat cagaaaataa
    33961 tcgaatgact cagcaagttt caacaagtgg gattgttcct caattattag gggctttggg
    34021 ggctattttt actgtagcag gtgttggtga tgttatctct catctgatta gcggtattgt
    34081 tccttcagat agtcgcttta taggagtttt ggcttatgtt cttggaatgg ttctattcac
    34141 aatgattatg ggaaatgctt ttgcagcatt caccgttatt acagcaggtg ttggagttcc
    34201 ctttgtattt gctctgggag ctaatccaat tgtggctggt gctcttgcca tgacagcagg
    34261 ttattgtggg accttattga ccccaatggc tgctaatttt aacgctctac cagcagcatt
    34321 gatggatatg aaagatcaga atggcgttat aaaggctcaa gcaggtgttg ctctagtaat
    34381 gattgttatt cacatattct taatgtactt tctcgcattt tagtaaagga aaataaaatg
    34441 aaaatattgg ttacaggttt taatcctttt ggaggtgaaa agattaatcc agctttggag
    34501 gctgtaaaat tattaccatc tgagattaat ggggctgaag ttcgctgggt agaaattcca
    34561 acggtttttt ataagtcgtc agaagtttta gaggcagaaa tattacgata tcaaccagat
    34621 gctgtacttt gtattggaca agcaggcggc aggaccggct taacacctga acgagtggct
    34681 attaatcaag atgatgctcg catacctgat aacgaaggca atcaaccaat tgatacaccg
    34741 attcgtattg atggagcatc ggcctatttt agtagtttac ctatcaaagc gatggtacaa
    34801 gctatcaaaa aacagggact tccggcagtt gtatccaata gtgcaggaac ctttgtttgc
    34861 aatcatttga tgtaccaagc tctttattta gtggataaaa aattccctaa tatgagagca
    34921 ggtttcatgc atattccata tatgatggaa caagtagtaa ataagccgaa tactgcagga
    34981 atgagtctat gtgatattgt aagaggcata gaggtagcta ttgaagctat tgtagactac
    35041 aaagataagg atttgcagtt agtaggcggt gagactcact gataacctaa aaaggatagt
    35101 caattatgct tgtttactaa ctattaacta tgctaaatca attgaggttg tttacataaa
    35161 actctatatc agagaagcct gatatagagt tttttcttgc tagttttagg attttcttgt
    35221 aaaatagaaa aagtgaagag aggtatgaaa tgagcaagaa agataaaaaa atcgaaattc
    35281 aagtagcgga tgccaaagtt aatgttggta aagacagttt tgaaggttat acattgacta
    35341 tcggtaaaaa agttatcgga gaaattgccg aattagacgg acaatttgcc attataaaga
    35401 atgggaatgt cgatagtttt tataaaaaat tggaaaaagc tgtggaaatt ttgattgaaa
    35461 attataattt agcaaaataa gtcttgtttt tttgaaattt tcatgatata atagtccatg
    35521 ttgattgtag gagagatagc gaagaggcta aacgcggcgg actgtaaatc cgctccttcg
    35581 ggttcggggg ttcgaatccc tctctctcca tttcattaat ggggtatagc caagcggtaa
    35641 ggcaagggac tttgactccc tcatgcgttg gttcgaatcc agctacccca gttcttaggt
    35701 aataatcaag atagaaagca aaatatctta gggtatttta tttttataat tgaaagacgt
    35761 gaatgatatg aacatgtcct tgcgggtgct taggaaaaaa attataagta tgtcaagttt
    35821 aagaaaaact tgattgttgg aggatttttt agatgaacga atttgaagat ttgctaaata
    35881 gcgttagtca agttgagact ggtgatgttg ttagtgctga agtattgaca gttgatgcga
    35941 ctcaagctaa cgttgcaatc tctggaactg gtgttgaagg tgtcttgact cttcgcgaat
    36001 tgacaaacga tcgtgatgca gatatcaatg actttgttaa agtaggagaa gtattggatg
    36061 ttcttgtact tcgtcaagta gttggtaaag atactgatac agttacatac cttgtatcta
    36121 aaaaacgcct tgaagctcgc aaagcatggg acaaacttgt tggtcgcgaa gaagaagttg
    36181 ttactgttaa aggaacgcgt gccgttaaag gtggactttc agtagaattt gaaggtgttc
    36241 gtggatttat cccagcttca atgttggata ctcgtttcgt acgtaacgct gagcgttttg
    36301 taggtcaaga atttgatact aaaatcaaag aagttaacgc taaagaaaac cgcttcatcc
    36361 tttcacgtcg tgaagttgtt gaagcagcta ctgcagcagc tcgcgctgaa gtattcggta
    36421 aattggctgt tggtgatgtt gtaactggta aagttgctcg tatcacaagc ttcggcgctt
    36481 tcatcgacct tggtggtgtt gacggattgg ttcacttgac tgaattgtca catgaacgta
    36541 acgtatcacc aaaatcagtt gtaactgttg gtgaagaaat tgaagtgaaa atccttgatc
    36601 ttaacgaaga agaaggacgt gtatcacttt cacttaaagc aacagtacca ggaccatggg
    36661 atggcgttga gcaaaaattg gctaaaggtg atgtagtaga aggaacagtt aaacgtttga
    36721 ctgacttcgg tgcatttgtt gaagtattgc caggtatcga tggacttgtt cacgtatcac
    36781 aaatttcaca caaacggatt gaaaatccaa aagaagctct taaagttggt caagaagttc
    36841 aagttaaagt tcttgaagtt aacgcagatg cagaacgcgt gtcactttct attaaagctc
    36901 ttgaagaacg tccagcccaa gaagaaggac aaaaagaaga aaaacgtgct gctcgtccac
    36961 gtcgtccaag acgtcaagaa aagcgtgatt tcgaacttcc agaaacacaa acaggatttt
    37021 caatggctga tttgtttggt gatatcgaac tttaatcaaa ttgaaaattc acaaaatcct
    37081 ttgtttacta aacaagggat ttttctggct ctttgtcaac tgtagtgggt tgaagtcagc
    37141 taagcttgag aaaggacaaa tttcgtcctt tcttttttga tgttcaaagc gataaaaatc
    37201 cgttttttga agttttcaaa gtttcgaaaa ccaaaggcat tgcgcttgat aagtttgatg
    37261 agattattgg tcgcttccag tttggcatta gaatagtgta gttgaagggc gttgataatc
    37321 ttttctttat ctttgaggaa ggttttaaag acagtctgaa aaataggatg agactgctta
    37381 agattgtcct caataagtcc gaaaaatttc tctggttcct tattctggaa gtgaaacagc
    37441 aagagctgat agtggtgttt caagtcttgt gaatagctca aaagcttgtc taaaatctct
    37501 ttattggtta aatgcatacg aaaagtagga cgataaaatc gcttatcact cagtttacgg
    37561 ctatcctgtt gtatgagctt ccagtagcgc ttgatagcct tgtattcatg ggattttcga
    37621 tgaaactgat tcatgttttg aacacgcaca cgactcatag cacggctaag atgttgcaca
    37681 atgtgaaagc gatcaagaac gattttagca ttcgggagtg aaacagtctg ggagactgtt
    37741 tcagcctgag cctagaaatt tgaaagcgaa gctgtttagc caagtcatag taagggctaa
    37801 acatatccat agtaataatt ttgacgcgac atcggacagc tctatcgtag cgaagaaagt
    37861 gatttcgaat gatagcttgt gttcttccct caagaacagt gatgatattg agattgttaa
    37921 aatcttgcgc aatgaagctc atctccatct cccgattgaa acagtcactc cccggactgt
    37981 ttcaacgtcc caggacataa tctcaggaag acgcgaaaaa tcatgctcaa agtgagaatc
    38041 attgagcttg cgaatgacag ttgaagttga gatggaaagc tgatgggcaa tatcagtcat
    38101 agaaatcttt tcaattaact tttgagcaat
//
LOCUS       DAVKJJ010000016        37904 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000016 DAVKJJ010000000
VERSION     DAVKJJ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37904
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.016"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            117..1142
                     /gene="trpS"
                     /locus_tag="VW144_000798"
     CDS             117..1142
                     /gene="trpS"
                     /locus_tag="VW144_000798"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU9600271.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            1294..2772
                     /gene="guaB"
                     /locus_tag="VW144_000799"
     CDS             1294..2772
                     /gene="guaB"
                     /locus_tag="VW144_000799"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU9600272.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(2823..3920)
                     /gene="recF"
                     /locus_tag="VW144_000800"
     CDS             complement(2823..3920)
                     /gene="recF"
                     /locus_tag="VW144_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEU9600273.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSDQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSKSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(3923..4291)
                     /gene="yaaA"
                     /locus_tag="VW144_000801"
     CDS             complement(3923..4291)
                     /gene="yaaA"
                     /locus_tag="VW144_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEU9600274.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            4450..5699
                     /locus_tag="VW144_000802"
                     /pseudo
     CDS             4450..5699
                     /locus_tag="VW144_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
     gene            5696..6979
                     /locus_tag="VW144_000803"
     CDS             5696..6979
                     /locus_tag="VW144_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9600275.1"
                     /translation="MTKVVFEEKYYPAVKEKVYRTRLANGLIVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEVTSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            7021..7842
                     /locus_tag="VW144_000804"
     CDS             7021..7842
                     /locus_tag="VW144_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9600276.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            7853..8398
                     /gene="pgsA"
                     /locus_tag="VW144_000805"
     CDS             7853..8398
                     /gene="pgsA"
                     /locus_tag="VW144_000805"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU9600277.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            8395..9222
                     /locus_tag="VW144_000806"
     CDS             8395..9222
                     /locus_tag="VW144_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9600278.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALDLVGMLDFKKREPARLSGGQKQRVAIAGVVVLRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSHNGYNLPENYLTESELEDKLWELL"
     gene            9207..10046
                     /locus_tag="VW144_000807"
     CDS             9207..10046
                     /locus_tag="VW144_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9600279.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            10039..10833
                     /locus_tag="VW144_000808"
     CDS             10039..10833
                     /locus_tag="VW144_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9600280.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKSMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            10892..11710
                     /gene="mreC"
                     /locus_tag="VW144_000809"
     CDS             10892..11710
                     /gene="mreC"
                     /locus_tag="VW144_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU9600281.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            11710..12204
                     /gene="mreD"
                     /locus_tag="VW144_000810"
     CDS             11710..12204
                     /gene="mreD"
                     /locus_tag="VW144_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU9600282.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVHDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            12298..13476
                     /locus_tag="VW144_000811"
     CDS             12298..13476
                     /locus_tag="VW144_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEU9600283.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQIGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            13700..14479
                     /gene="rpsB"
                     /locus_tag="VW144_000812"
     CDS             13700..14479
                     /gene="rpsB"
                     /locus_tag="VW144_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU9600284.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            14558..15598
                     /locus_tag="VW144_000813"
     CDS             14558..15598
                     /locus_tag="VW144_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEU9600285.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            15844..16131
                     /gene="tnpB"
                     /locus_tag="VW144_000814"
     CDS             15844..16131
                     /gene="tnpB"
                     /locus_tag="VW144_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9600286.1"
                     /translation="MIIQLSDLGQVHLVCGKTDMRQGIDSLAYVVKTHFELDSFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENGKLTWLSTEKDVKALAPEQVD"
     gene            complement(16322..16630)
                     /locus_tag="VW144_000815"
                     /pseudo
     CDS             complement(16322..16630)
                     /locus_tag="VW144_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            16621..17019
                     /locus_tag="VW144_000816"
     CDS             16621..17019
                     /locus_tag="VW144_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9600287.1"
                     /translation="MKAPIPKAPLAHSLGSASIIAHTIHQKFNLKVPNYRQEEDWAKM
                     GLPITRKEIANWHIKASQYYLEPLYNLLRERLLTQSLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(17216..18136)
                     /gene="cysK"
                     /locus_tag="VW144_000817"
     CDS             complement(17216..18136)
                     /gene="cysK"
                     /locus_tag="VW144_000817"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEU9600288.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(18421..19056)
                     /locus_tag="VW144_000818"
     CDS             complement(18421..19056)
                     /locus_tag="VW144_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEU9600289.1"
                     /translation="MEFRTIKEDDQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            19112..20410
                     /locus_tag="VW144_000819"
     CDS             19112..20410
                     /locus_tag="VW144_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9600290.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            20407..21069
                     /locus_tag="VW144_000820"
     CDS             20407..21069
                     /locus_tag="VW144_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEU9600291.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVGVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            21149..21697
                     /gene="raiA"
                     /locus_tag="VW144_000821"
     CDS             21149..21697
                     /gene="raiA"
                     /locus_tag="VW144_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU9600292.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            21833..23806
                     /locus_tag="VW144_000822"
     CDS             21833..23806
                     /locus_tag="VW144_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU9600293.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTSLLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSKAGEKLTEIVLNEIKEKEKEE"
     gene            23803..24255
                     /gene="rplI"
                     /locus_tag="VW144_000823"
     CDS             23803..24255
                     /gene="rplI"
                     /locus_tag="VW144_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU9600294.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            24299..25651
                     /gene="dnaB"
                     /locus_tag="VW144_000824"
     CDS             24299..25651
                     /gene="dnaB"
                     /locus_tag="VW144_000824"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU9600295.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            25653..25919
                     /locus_tag="VW144_000825"
     CDS             25653..25919
                     /locus_tag="VW144_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600296.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            26008..27354
                     /gene="cbpD"
                     /locus_tag="VW144_000826"
     CDS             26008..27354
                     /gene="cbpD"
                     /locus_tag="VW144_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEU9600297.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEMPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGHSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGYKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      27437..27539
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            27610..27900
                     /locus_tag="VW144_000827"
     CDS             27610..27900
                     /locus_tag="VW144_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEU9600298.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQGADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            27863..28621
                     /locus_tag="VW144_000828"
     CDS             27863..28621
                     /locus_tag="VW144_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9600299.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            28660..29667
                     /locus_tag="VW144_000829"
     CDS             28660..29667
                     /locus_tag="VW144_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9600300.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            29667..30395
                     /locus_tag="VW144_000830"
     CDS             29667..30395
                     /locus_tag="VW144_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9600301.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            30514..30972
                     /locus_tag="VW144_000831"
     CDS             30514..30972
                     /locus_tag="VW144_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEU9600302.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            30974..33406
                     /locus_tag="VW144_000832"
     CDS             30974..33406
                     /locus_tag="VW144_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9600303.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            33715..34368
                     /gene="rr06"
                     /locus_tag="VW144_000833"
     CDS             33715..34368
                     /gene="rr06"
                     /locus_tag="VW144_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEU9600304.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKEVLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            34365..35705
                     /gene="hk06"
                     /locus_tag="VW144_000834"
     CDS             34365..35705
                     /gene="hk06"
                     /locus_tag="VW144_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEU9600305.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            35788..36315
                     /locus_tag="VW144_000835"
     CDS             35788..36315
                     /locus_tag="VW144_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEU9600306.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYMTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            36394..>37904
                     /gene="pspC"
                     /locus_tag="VW144_000836"
     CDS             36394..>37904
                     /gene="pspC"
                     /locus_tag="VW144_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HEU9600307.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVAVASLFLGGVVHAEGVGGRN
                     TSTVTSSGQDTSKKYADEVKSHYQSILEKVRKSLEKDRHTQNVGLITKLSEIKKKYLY
                     ELEVNVLLEEKSKAELPSKAKAELDAAFEQFKKEPELTKKVAEAQKKVEEAKKKAEDQ
                     KEEDFRNYPTNTYKTIELEIAESDVKVKEAELELLKEEAKEHRDEGTIKQVEEKVKSE
                     KAEATRLEEIKTERKKAEEEAKRRADAKEQGKPKGRAKRGVPGEQATPDKKENDAKSS
                     DSSVGEETLPNPSLKPEKKVAEAQKKVEEAKKKAKDQKEEDHRNYPTITYKTLELEIA
                     ESDVEVKKAELELVKEEAKGSRNEEKVKQAKAEVESKKAEATRLEKIKTDRKKAEEAK
                     RKAAEEDKVKEKPAEQPQPAPAPQPEKPAPAPKPENPAEQPKAEKPADQQAEEDYARR
                     SEEEYNRLTQQQPPKTEKPAQPSTPKTGWKQENGMWYFYNTDGSMATGWLQNNG"
BASE COUNT        11905 a         6164 c         8384 g        11451 t
ORIGIN      
        1 tttacagtat cctttacaca aatctgtaaa aagcaaggct gatttatttt gataaattac
       61 ggttatttca ttaaaaaaat gctataattg aaaggactat atcgaaggag aacaaaatga
      121 ctaaacccat tattttaaca ggagaccgtc caacaggaaa attgcatatt ggacattatg
      181 ttggaagtct caaaaatcga gtattattac aggaagagga taagtatgat atgtttgtgt
      241 tcttggctga ccaacaagcc ttgacagatc atgccaaaga tcctcaaacc attgtagagt
      301 ctatcggaaa tgtggctttg gattatcttg cagttggatt ggatccaaat aagtcaacta
      361 tttttattca aagccagatt ccagagttgg ctgagttgtc tatgtattat atgaatctag
      421 tttcgttagc acgtttggag cgaaatccaa cagtcaagac agagatttct cagaaaggat
      481 ttggagaaag cattccgaca ggattcttgg tctatccaat cgctcaagca gctgatatca
      541 cagctttcaa ggctaattat gttcctgttg ggacagatca gaaaccaatg attgagcaaa
      601 ctcgtgaaat tgttcgttct tttaacaatg catataactg tgatgtcttg gtagagccgg
      661 aaggtattta tccagaaaat gagagagcag ggcgtttgcc tggtttagat ggaaatgcta
      721 aaatgtctaa atcactaaat aatggtattt atttagctga tgatgcggat actttgcgta
      781 aaaaagtaat gagtatgtat acagatccag atcatatccg cgttgaggat ccaggtaaga
      841 ttgagggaaa tatggttttc cattatctag atgtttttgg tcgtccagaa gatgctcaag
      901 aaattgctga tatgaaagaa cgttatcaac gaggtggtct tggtgatgtg aagaccaagc
      961 gttatctact tgaaatatta gaacgtgaac tgggtcctat tcgtgagcgc cgtattgaat
     1021 ttgctaagga tatgggagaa gtttataata tgattcaaaa aggtagtgaa agagcgcgtg
     1081 aagttgcggg tcaaacccta tctgaggtaa aaggggcaat gggacttcat tactttaact
     1141 aagtttattt tacaagaaac tatcatttct atctcgaaga aaagatagtt ttttatttac
     1201 ccctgtaaca tttgacaaat ttgtatagaa tggtatgata gatacaatat aaaaagagtc
     1261 aagctcaaaa agaaagaaaa gaggaaactt cgaatgtcta attgggacac taaatttttg
     1321 aaaaaaggtt ttacctttga tgatgtattg cttattccag ctgaaagtca tgtgttgcct
     1381 aacgatgcag atttaacaac taaattggca gataatttga ctttaaatat cccaattatt
     1441 accgctgcca tggacacagt tacagagagt caaatggcca ttgctattgc tcgtgcaggt
     1501 ggtctcggag ttatccataa aaacatgtca attgctcaac aagcagacga ggttcgtaag
     1561 gtaaaacgtt ctgaaaatgg agttattatt gatccgttct tcttgacgcc tgaacataca
     1621 attgctgaag cagatgagct tatgggtcgt taccgcatca gtggtgttcc agttgttgaa
     1681 acacttgaaa atcgtaaatt ggttggtatt ttgacaaacc gagatcttcg ttttatttca
     1741 gattataatc aaccaatttc aaaccatatg actagtgaaa atcttgttac tgctcctgtg
     1801 ggtacggatc ttgcaacggc tgagagtatt cttcaagagc atcgtattga aaaacttccg
     1861 ttggtcgatg aagaaggcag tctttctggt ttgatcacta tcaaagatat tgaaaaagtt
     1921 attgagtttc caaatgcggc taaagatgag tttggtcgtc ttctagttgc aggtgcagta
     1981 ggtgttactt cagatacatt tgaacgtgca gaggctcttt ttgaggcagg agcggatgcg
     2041 attgttattg atactgcaca tggtcattct gcaggtgtct tgcgtaaaat tgccgagatt
     2101 cgtgctcatt tcccagatcg gactttgatt gctggaaata ttgctactgc tgaaggtgca
     2161 cgtgcccttt atgaagcggg tgtagacgtt gttaaggttg gtattggacc aggttctatc
     2221 tgtactactc gtgtgattgc tggtgttggt gttccgcaag taacagctat ctacgatgct
     2281 gcagctgttg cgcgcgaata tggtaaaacg attattgctg acggtgggat caagtattct
     2341 ggagatattg taaaagcact tgctgcaggt ggaaatgctg ttatgcttgg atctatgttt
     2401 gctggaactg atgaagctcc aggcgaaact gaaatcttcc aaggacgtaa attcaagact
     2461 taccgtggta tgggatcaat tgctgctatg aagaaaggtt caagcgaccg ttatttccaa
     2521 ggttctgtca atgaagcaaa caagcttgtt ccagaaggaa ttgaaggtcg tgttgcttat
     2581 aaaggagcgg cagctgatat tgttttccaa atgattggtg gtattcgctc tggtatgggt
     2641 tactgtggtg cagctaacct taaagaacta cacgataatg ctcaatttat tgaaatgtct
     2701 ggtgctggtt tgaaagaaag ccatcctcat gatgtgcaaa ttactaatga ggcaccaaat
     2761 tattctatgt aaaaaacaat gaaaagaact ccagtgaaaa caggagttct tttataatgt
     2821 tgtcaatttc catttacagc agctttacca tcctgaatag tgaagatact tagattttct
     2881 ggcagatttt gaagatggtc taagcttgtt gttgtgataa aggtttggat tgattgagaa
     2941 atcgtttcta ataattttaa ctgtctagtg ttgtcaagtt cactcatcac atcgtcaagc
     3001 agtaatatcg gagattctgt ggtaatgctt tccattaatt cgatttctgc taattttatc
     3061 gagaggacga gactacgatg ttgaccttgg cttccgaaac tagcatccat cccatttata
     3121 taaaaagaaa tgtcatctcg atgaggaccg acaccagtat tctttttaaa taaatctctg
     3181 gatttacttt tttctaaagc aattttgaaa gattcggata agttttgttt gtcagttata
     3241 ttgacagaag attgatagga tattgacaac tcttcgatct gattagagag ttcaaaatgt
     3301 ttcttacgcc caaatgattc tagttttttt atgaaatcta agcggtgatt cattacacga
     3361 catccataat caactagctg atcatctaac acagaaagga atgtttcatc tattttttga
     3421 tctgatttta ggtaagtgtt tctttgcttt aggatgtggt tataattggt taagtcagat
     3481 aagtagattg gcttaatttg cccaagttcc atatcaatga attttcgtcg aatcgaaggt
     3541 gctcctttaa ttagttgtaa atcttcagga gcaaataaga caacattcat gtgtcctaca
     3601 taatctgaaa ggcgtgcctg ttttaagtga ttaacttttg ttacacggcc tttttgtgtt
     3661 agttcgattt ctaggggaat ggatccagtt tttttctgaa cgagacctga aagatgaagt
     3721 tgttcctcat caaaatgaat gagattttta tctgttcgag ttcgatgact acgcgttaag
     3781 gctaaaaaat agatagcctc taacatgttt gtttttcctt gtgcattacg tcccaaaaag
     3841 acattcaatt taggattaaa gtctattttc gtttctttgt aattacgaaa agtcttgaga
     3901 gatagatgtt gtagccacat gattatctac cagggaatcg tggagcttgt tggcttttag
     3961 gtgatgaagc aggtttcgat ttgtcttttt taactccctt attcatcttt ttgacaagtt
     4021 tagcgatccg ttctttttca actttatcag cttggtattc atcttgttct tcagaagtag
     4081 gttgtgtcaa caagatgtca atgttcatgt cagggatgtc aactttatcg ccaatacgaa
     4141 gtttttttcc acgacgactc tctaattccc tattaaagta aacagaatgt tcagagagaa
     4201 atgatttaat agctcctccg ctatgtgtaa ttccaagttc tttgagtagt gcttggaggg
     4261 taataaattc ttcaaataat ttgtattcca taattcacct caatctttgg tattatacca
     4321 tattttccta aaaatagtga gagagacaga gagaaatgta agcgattttt atttcaaaag
     4381 tgttacaggg aataagaaaa tttcaggttt tcgtgatata atagaagtct gtatataagg
     4441 aggtaaatca tggagttagt gcatggaatt tcaacacatt ttatccaatc aaaaaagttt
     4501 aaaacaaaca aaattaccgt gcgttttacc gctccattat cccttgatac gattgcaggt
     4561 cacatgttga gtgcaagtat gctagagact gctaatcaga tgtaccccac ttctcaagat
     4621 ttgaggagac acttggccag tctatacggt acagatatgt caaccaattg tttcagaaga
     4681 gggcaaagcc acattataga attgacattt acctatgttc gtgatgagtt tttaagtagg
     4741 aaaaacgtgc taacctctca gattttggaa cttgtaaaag aaactctttt ttcacccgta
     4801 gtagttgata atgggtttga tccggcctta tttgaaattg agaaaaaaca attgctagca
     4861 agtttagcag ctgatatgga tgattctttt tattttgcac ataaagaatt ggataaattg
     4921 ttttttcatg atgaacgtct tcaattggaa tatagtgatt tacgaaatcg tattttagct
     4981 gaaactccac aaagttctta ttcttgtttc caagaatttt tagccaatga tcgaatagat
     5041 ttctttttcc taggtgattt taatgaggtt gaaattcaaa atgtattaga atcatttggc
     5101 tttaaaggtc gaaaaggaga tgtgaaggtt cagtattgtc aaccttattc taatatcctt
     5161 caggaaggta tggttcggaa aaatgtggga caatccattt tggaattagg ttatcattac
     5221 cgttctaaat atggtgatga gcaacattta cccatgattg taatgaatgg tttacttggt
     5281 ggatttgctc attctaagct ctttacaaat gtccgtgaaa atgctggatt agcttatacc
     5341 atttcaagtg agcttgattt atttagtgga ttcttgagga tgtatgctgg tatcaatcga
     5401 gaaaatcgta accaggctcg taaaatgatg aataatcaac tgcttgattt aaaaaaggtt
     5461 attttacaga gtttgagtta aatcagacca aggaaatgat tcgttggtcg ttgttacttt
     5521 ctcaagataa tcaatcttca ttgattgaac gtgcttatca aaatgcctta tttggaaaat
     5581 cttcagcaga ctttaaaagt tggattgtaa agcttgaaca gattgacaaa gatgctattt
     5641 gtagagtagc taataatgtg aaactacaag cgatttactt tatggaagga atagaatgac
     5701 aaaggttgtt tttgaagaaa aatactatcc agctgtaaaa gaaaaggttt atcgaactcg
     5761 tttggccaat ggattgatag ttgctctttt gcctaaaaag gaatttaaag aggtttacgg
     5821 gagtgtcact gtacagtttg gttcggtaga tacgtttgtc acagaagttg acggagatgt
     5881 aaaacaatat cctggaggaa ttgctcattt tcttgaacat aaattatttg agagagaaga
     5941 ttctagtgat ttgatgtcgg cttttacgag tctaggtgca gatagtaatg cctttacaag
     6001 ctttacaaaa acaaactatc ttttttcagc aacggattat tttttagaaa atttagattt
     6061 acttgatgaa ttggtaacat cagcacactt tactgaagct tccattctga cagagcagga
     6121 tattattcag caagaacgag aaatgtacca agatgatcca gattcgtgtt tattcttttc
     6181 aactttagcg aatttgtatc ctggtacacc tttagcaact gatatagttg gaagtgagga
     6241 gtccatttcc caaatcaatc taactaattt gcaagaaaat tttacaaagt tttacaaacc
     6301 tgtaaacatg tctctgtttt tagttggtaa ttttgatgtg gagcgagtac aggactattt
     6361 tgaaagcaaa gaactgaaag attcagattt tcaggaagta gcaagagaaa agttgttttt
     6421 acagcctgta aagccaacag atagtatgag aatggaagta tcttctccca aactagcgat
     6481 tggagttaga ggtaagcgag aagtttctga agcggattgc tatcgacatc atattttatt
     6541 aaagttattg tttgcaatga tgtttggttg gacttcggat cgttttcaaa aatgttatga
     6601 atcaggtaaa attgatgcat ccttatctct ggaagttgaa gtaacaagtc gctttcattt
     6661 tgtcatgttg acaatggata cgaaagagcc agttgctttg tctcatcaat ttaggaaggc
     6721 tattcgtaat tttacaaagg atttagatat tacagaggaa catttagata ttatcaaaag
     6781 agagatgttt ggcgaatttt tcagtagcat gaactctctt gaatttattg caacgcaata
     6841 tgatgctttt gaaaatggtg agacaatttt tgatttgccg aaaattttac aggaaattac
     6901 tttagaggat gtccttgatg ctggacatca tttaatagat gatggtgaca tagttgattt
     6961 tacaatattc ccatcgtagt aacctattat aatagacact agaaagaagg gatgacaagt
     7021 atgagaaaaa aaacaattgg agaggtttta cgattagcta gaatcaatca gggattgagt
     7081 ttagatgaat tgcagaaaaa gacagaaatc cagttagata tgttggaagc aatggaagca
     7141 gacgatttcg atcaacttcc aagtcctttt tacacgcgtt ctttcttgaa aaaatatgca
     7201 tgggctgttg agttagatga ccaaattgtt ttggatgctt atgattctgg gagtatgatt
     7261 acttatgagg aagtagatgt tgatgaagat gagttgacag gtcgtagacg ttcaagtaag
     7321 aaaaagaaga aaaaaacatc atttttacct ttattttatt ttatcctttt tgctttatcg
     7381 attttaattt ttgtgactta ttatgtttgg aactatattc aaactcaacc agaggagcct
     7441 tctctttcta attacagtgt ggttcaatca acaagttcaa ctagctctgt tccccactcc
     7501 tcaagtagta gttcttctag tatagaatca gctataagtg tatcaggcga aggaaatcat
     7561 gtagaaatcg cttataagac aagtaaggaa acagttaaat tgcaattggc agtttcagat
     7621 gttacaagtt gggtcagtgt ttcagaaagc gaacttgagg gcggtgtaac cttatcgcca
     7681 aagaagaaaa gtgcagaagc aacagttgca actaaaagtc ctgtaacaat tacgttaggt
     7741 gttgtaaaag gtgttgattt gacagtagat aatcagactg ttgatttatc gaaattaaca
     7801 gctcagactg gacaaatcac tgtaaccttt actaaaaatt aaggaaaaac gaatgaaaaa
     7861 agaacaaatt cccaatctct taacaatagg tcgaattctc tttataccta tttttatctt
     7921 tattttaacg ataggaaatt cgatagagag tcatatagtt gcagctatta tctttgctgt
     7981 tgccagtatt accgactatt tagatggata tttagctcgt aaatggaatg tggtcagtaa
     8041 ttttggtaaa tttgcagatc ctatggcgga taagttacta gttatgtcgg cttttattat
     8101 gttgattgag ttaggtatgg ctccggcttg gattgttgca gtgattatct gtcgtgagtt
     8161 agctgtgaca ggtttaaggc ttttattggt tgaaactggt ggaacaattt tagcagcagc
     8221 aatgcctgga aaaattaaaa cttttagtca gatgtttgcc attattttct tgctattaca
     8281 ttggactttg cttggtcaag ttctacttta tgtagcctta tttttcacta tctactctgg
     8341 ctatgactat ttcaagggta gtgcctatgt atttaaaggg acatttggtt cgaaatgaaa
     8401 tcaataattg atgtaaaaaa tctttctttt cgctataaag aaaatcagaa ctactacgat
     8461 gtgaaggata ttacgtttca cgtgaaacgt ggagaatggc tttcgattgt agggcataat
     8521 ggtagtggta aatcaacgac ggttcgatta attgatggct tactggaagc agaatccgga
     8581 gagattgtaa ttgatggcca acgtttgact gaggaaaatg tttggaatat acgtcgtcaa
     8641 atcggtatgg tttttcaaaa tccagacaat caatttgttg gagcgactgt tgaagatgat
     8701 gttgcttttg gtttggaaaa tcagggactt tctcgtcaag aaatgaaaaa gagagtagaa
     8761 gaagctctgg atttagttgg catgttggac tttaaaaaga gagagccagc gcgtctatca
     8821 ggtggccaaa agcaacgtgt ggccattgca ggtgttgtag tcctaagacc agctatttta
     8881 atcttagatg aagcaacgag tatgttggat cctgaggggc gtagagaact tattgggaca
     8941 gtaaaaggaa ttcgaaaaga ctatgatatg acggtcattt ctattaccca tgatttggaa
     9001 gaagtcgcca tgagtgatcg cgtattggtc atgaaaaaag gggaaattga atcaactagt
     9061 agtccaaggg agcttttctc tcgaaatgat ttagatcaaa ttggattaga cgatcctttt
     9121 gctaatcaat taaaaaaatc tttgagccat aatggctata atttacctga aaattatttg
     9181 acagaaagtg agctagagga taagctatgg gaattgctct agaaaatgtg aattttacat
     9241 atcaagaagg tactccctta gcttcagcag ctttgtcgga tgtttctttg acgattgaag
     9301 atggctctta tacagcttta attgggcaca caggtagtgg taaatcaact attttacaac
     9361 tcttaaatgg tttattggtg ccaagtcaag ggagtgtgag ggtttttgat accttaatca
     9421 cctcgacttc taaaaataaa gatattcgtc aaattagaaa acaggttggc ttggtatttc
     9481 agtttgctga aaatcagatt tttgaagaaa cggttttgaa ggacgttgct tttggaccgc
     9541 aaaattttgg agtttctgaa gaagatgctg tgaagactgc gcgtgagaaa ctggctctgg
     9601 ttggaattga tgaatcactt tttgatcgta gtccgtttga gctgtcaggg ggacaaatga
     9661 gacgtgttgc cattgcaggc atacttgcca tggagccagc tatattagtc ttagatgagc
     9721 caacagctgg tctagatcct ctagggagaa aggagttgat gaccctgttc aaaaaactcc
     9781 accagtcagg gatgaccatc gtcttggtaa cgcatttgat ggatgatgtt gctgaatatg
     9841 cgaatcaagt ctatgtaatg gaaaagggac gtttagtaaa ggggggcaaa ccaagtgatg
     9901 tctttcaaga cgttgttttt atggaagaag ttcagttggg agtacctaaa attacggcct
     9961 tttgtaaacg attggctgat agaggcgtgt catttaaacg attaccgatt aagatagagg
    10021 agttcaagga gtcgctaaat ggatagtatg attttagggc gttatatccc aggggattcg
    10081 attgttcacc gattggatcc acgtagcaaa ttgcttgcta tgatgctact gattttgatt
    10141 gtgttttggg ctaataaccc cttgacgaat ctgattcttt ttatagcgac agggatattt
    10201 attgccttgt caggagtatc tctttcattt tttattcagg gcttgaagtc tatgtttttc
    10261 ttgattgcct tcacaactat ttttcaacta tttttcattt ctaatgggaa tgttttattt
    10321 gagttttcgt ttgtgagaat cacggattat gctttgcaac aagctgggat tattttttgt
    10381 cgctttgtat tgattatttt cttttcaact ttgttaacct taacaaccat gcctttaagt
    10441 ttggcatcag ctgtcgaagc tttattagca cctttaaagc gtgtgaaagt tccagttcat
    10501 gaaattggat tgatgctgtc tatgagtttg cgttttgtcc caaccttgat ggatgatacg
    10561 acgcggatta tgaatgcaca gaaagctcgt ggagtggatt ttggagaagg aagcatcgtt
    10621 caaaaagtaa agtcgatgat tcccattttg attcctcttt ttgcgacaag tttaaaacgt
    10681 gcagattcct tggctatcgc tatggaagcg cgtggctatc agggtggaaa aggcagaagt
    10741 caataccgac aattgaaatg gactctaaag gatacgctga ccattcttgt tattctcgta
    10801 cttggttgtt gtttattttt cttaaaatct tagtagcttt caggaattga taaaaagtta
    10861 ctgtaacagt ttttgatata aaggtaggga tatgaaccgt tttaaaaaat caaaatatgt
    10921 cattattgtt tttgtcactg ttctgcttgt gtcagctctc ttagcgacga cttattcaag
    10981 tacaattgtg acaaaattag gagatggaat ctcattggtt gatagagttg tacaaaaacc
    11041 ttttcagtgg tttgattctg tcaaatcaga tttggctcat ttgacacgaa catataatga
    11101 aaatgaaagt ttgaagaaac agctttacca attagaagtt aaatcaaatg aggtggaaag
    11161 tttaaagaca gaaaatgaac aactgcgcca attgcttgat atgaagtcta aattgcaagc
    11221 cacaaagact ttagcagcag atgttattat gcgttctccg gtatcttgga agcaggagtt
    11281 gaccttagat gcaggtagat caaaaggtgc ttctgagaac atgttagcta ttgcaaatgg
    11341 tggcttgatt gggagtgttt caaaagtaga ggagaactct actatagtta accttctgac
    11401 aaatacggaa aatgctgata agatttctgt taaaattcaa catggctcta ctacaattta
    11461 tggaattatt attggctatg acaaggaaaa tgacgttctt aaaattagcc aattaaatag
    11521 taatagcgat attagtgcgg gagataaggt gactactggt ggattaggaa actttaacgt
    11581 tgctgatatt cctgttggtg aagtggttgc cacaacgcat agtacagact atttgacacg
    11641 agaagtaact gttaaattga gtgcagatac tcataatgta gatgtgatag aattagtggg
    11701 gaattcataa tgagacagtt gaagcgagtt ggagtatttt tattgcttcc tttctttgtt
    11761 ctaattgacg cccatattag ccagcttctg ggctcatttt tcccccatgt acatttggct
    11821 agtcattttc tttttctatt tctcttattt gagacgatag aagtatcaga gtatctctac
    11881 ctagtctatt gttttgttat aggcttggtt catgatgttt actttttcca tctaataggg
    11941 attacaactc tcttatttat cttattggga gccttccttc ataaattgaa tagtgttatt
    12001 ttgttgaatc gttggacaag aatgctagct atgattgtgc tgacattcct gtttgaaatg
    12061 ggtagttatc ttttggcttt tatggtaggg ttgacagtag atagcatgtc gatttttata
    12121 gtctatagct tggtaccgac gatgatttta aattttttat ggattactgt ttttcaattt
    12181 atttttgaaa aatattatct ataagaacga catataaatg taacaaaggc gtaatattta
    12241 ttaggccttt ttttggtata ctagtattgt ctttaaaaga aggagtatct acgtaatatg
    12301 aagaaaaaaa tcttagcgtc acttttatta agtacagtaa tggtttctca agtagctgtt
    12361 ttaacaactg cgcatgcaga aacgactgat gacaaaattg ctgctcaaga taataaaatt
    12421 agtaacttaa cagcacaaca acaagaagcc caaaaacaag ttgaccaaat tcaggagcaa
    12481 gtatcagcta ttcaagctga gcagtctaac ttgcaagctg aaaatgatag attacaagca
    12541 gaatctaaga aactcgaggg tgagattaca gaactttcta aaaacattgt ttctcgtaac
    12601 caatcgttgg aaaaacaagc tcgtagtgct caaacaaatg gagccgtaac tagctatatc
    12661 aataccattg taaactcaaa atcaattaca gaagctattt cacgtgttgc tgcaatgagt
    12721 gaaatcgtat ctgcaaacaa caaaatgtta gaacaacaaa aggcagataa aaaagctatt
    12781 tctgaaaaac aagtagcaaa taatgatgct atcaatactg taattgctaa tcaacaaaaa
    12841 ttggctgatg atgctcaagc attgactacg aaacaggcag aactaaaagc tgctgaatta
    12901 agtcttgctg ctgagaaagc gacagctgaa ggggaaaaag caagtctatt agagcaaaaa
    12961 gcagcagctg aggcagaggc tcgtgcagct gcggtagcag aagcagctta taaagaaaaa
    13021 cgagctagcc aacaacaatc agtacttgct tcagcaaaca ctaacttaac agctcaagtg
    13081 caagcagtat ctgaatctgc agcagcacct gtccgtgcaa aagttcgtcc aacatacagt
    13141 acaaacgctt caagttatcc aattggagaa tgtacatggg gagtaaaaac attggcacct
    13201 tgggctggag actactgggg taatggagca cagtgggcta caagtgcagc agcagcaggt
    13261 ttccgtacag gttcaacacc tcaaattgga gcaattgcat gttggaatga tggtggatat
    13321 ggtcacgtag cggttgttac agctgttgaa tcaacaacac gtatccaagt atcagaatca
    13381 aattatgcag gtaatcgtac aattggaaat caccgtggat ggttcaatcc aacaacaact
    13441 tctgaaggtt ttgttacata tatttatgca gattaattta cagagggact cgaatagagc
    13501 cctcttttca ggttttaccg tgacaatccc tattaaaaat tatatcaaaa tagcttgaaa
    13561 atattggaaa agtatggtag aatgaaaatt gtcgtgtgaa cgataatact cattcttgat
    13621 gaattgtgaa gcagttgccc ttgggtcgtt ttgcgagttg aagtcaagaa gaggaaaaaa
    13681 acaaaaagga gaaatactca tggcagtaat ttcaatgaaa caacttcttg aggctggtgt
    13741 acactttggt caccaaactc gtcgctggaa tcctaagatg gctaagtaca tctttactga
    13801 acgtaacgga atccacgtta tcgacttgca acaaactgta aaatacgctg accaagcata
    13861 cgacttcatg cgtgatgcag cagctaacga tgcagttgta ttgttcgttg gtactaagaa
    13921 acaagcagct gatgcagttg ctgaagaagc agtacgttca ggtcaatact tcatcaacca
    13981 ccgttggttg ggtggaactc ttacaaactg gggaacaatc caaaaacgta tcgctcgttt
    14041 gaaagaaatt aaacgtatgg aagaagatgg aactttcgaa gttcttccta agaaagaagt
    14101 tgcacttctt aacaaacaac gtgcgcgtct tgaaaaattc ttgggtggta tcgaagatat
    14161 gcctcgtatc ccagatgtga tgtacgtagt tgacccacat aaagagcaaa tcgctgttaa
    14221 agaagctaaa aaattgggaa tcccagttgt agcgatggtt gacaccaata ctgatccaga
    14281 tgatatcgat gtaatcatcc cagctaacga tgacgctatc cgtgctgtta aattgatcac
    14341 agctaaattg gctgacgcta ttatcgaagg acgtcaaggt gaggatgcag tagcagttga
    14401 agcagaattt gcagcttcag aaactcaagc agattcaatt gaagaaatcg ttgaagttgt
    14461 agaaggtgac aacgcttaat ttatacaaat agtaattacc taggagggcg gggcttagcc
    14521 cggctctcct attttcaaaa aatataggag aattaaaatg gcagaaatta cagctaaact
    14581 tgtaaaagag ttgcgtgaaa aatctggtgc cggtgttatg gacgctaaaa aagcgcttgt
    14641 agaaacagac ggtgacatcg aaaaagcgat tgaattgctt cgtgaaaaag gtatggctaa
    14701 ggcagctaag aaagctgacc gtgttgctgc agaaggtttg actggtgttt atgttaacgg
    14761 taatgttgca gcagttattg aagtaaacgc tgaaactgac ttcgttgcaa aaaacgctca
    14821 attcgttgaa ttggtaaata ctacagctaa agtcattgct gaaggaaaac ctgctaacaa
    14881 tgaagaagct cttgctttga taatgccttc aggtgaaact cttgaagctg catacgtatc
    14941 tgcaacagca actatcggag agaaaatctc attccgtcgc tttgcattga ttgaaaaaac
    15001 agacgcacaa cactttggag cataccaaca taacggtgga cgtatcggtg ttatttcagt
    15061 tgttgaaggt ggagacgaag cacttgctaa acaattgtca atgcacatcg cagcgatgaa
    15121 accaacagtt ctttcttaca aagaattgga tgagcaattc gttaaagatg agttggcaca
    15181 attgaatcac gttatcgacc aagacaacga aagccgtgca atggttaata aaccagctct
    15241 tccacacttg aagtatggat caaaagctca attaactgat gatgttattg ctcaagctga
    15301 agctgacatc aaagctgaat tggctgcaga aggcaaacca gaaaaaatct gggacaaaat
    15361 tattccaggt aaaatggatc gcttcatgct tgataacact aaagttgacc aagcttacac
    15421 acttcttgca caagtttaca tcatggatga cagcaagaca gttgaagcat accttgaatc
    15481 agttaacgct tcggtagttg agtttgctcg ctttgaagtt ggtgaaggta tcgagaaagc
    15541 tgcaaacgac ttcgaagctg aagttgcagc tacaatggca gcagccttga ataactaata
    15601 atagaaagga agcaaattgc ttcctttttt gtatagggct tagatttttc ctgaaattgt
    15661 tttttcccca gtctcactcg attagaataa ttaaaacaaa aagccctata ataagggcta
    15721 agtgcattta ggagactata cttaaaattc atagtaagcg cgtcataaca aggtatctat
    15781 cctaattccc caacttccag tatactagat aaaaaataat tatcggagga aaggtattgt
    15841 actatgataa ttcaactaag tgatttaggt caagttcacc ttgtttgtgg caagacagat
    15901 atgaggcagg gaatagactc attagcctat gtagttaaaa cccactttga attggattct
    15961 ttctccggtc aagtctttct cttttgtggt ggacgtaaag accgctttaa agccctttac
    16021 tgggatggtc aaggattttg gctactatat aaacgctttg agaacggaaa actgacttgg
    16081 ctaagtacag aaaaggatgt caaagctctc gcacctgaac aagtagacta gcttatgaag
    16141 ggcttttcta tcactccaaa aatatagtag attgaaacta gaatagtaca cctctgcttt
    16201 taaaacattg ttagaaatcg atttgactgt cctgatcgat ttgtcctgtt cttatttcat
    16261 tttactataa atccatcaga aagtcgtgat ttctattgaa atgaggactt tctttttata
    16321 ctcatctgtt ttcaaaaagc attctagtcc atctccgatt aacgatggac tttatcacct
    16381 ccttctccag tccttgtata acatcttgga gttgattcat gacatcttcc aaagtttgaa
    16441 aggctttatt cttaaatcca cgtttacgaa tctctttcca cacttgttca atggggttca
    16501 tctctggtgt gtatggagga ataaaggtaa aaccaatatt agtcggaatc tttaaggtac
    16561 ttgatttatg ccatatagca ttgtccataa cgagtaaaag ataatcatct ggataagctt
    16621 gtgaaagctc ctattcctaa agcccctttg gcgcatagcc ttggctcagc ttctattatc
    16681 gctcacacca tccatcagaa gtttaatctg aaggtaccca attatcgcca agaagaagat
    16741 tgggctaaga tgggtttacc aatcacacgt aaggaaattg ctaattggca tatcaaggcg
    16801 agtcaatact atttggagcc cctttataac ctcttgcgag agagactatt gacgcagtcc
    16861 ttacttcatg cggatgaaac ttcttatagg gtgctagaga gtgatagtca gctgacttac
    16921 tattggactt ttttgtctgg gaaagctgag aaacaaggga ttacgcttta ccatcatgtc
    16981 ctaatcgatt tgttcatatc ttatttcaat ccattataaa tagtgagaaa tatctatcct
    17041 atcttctaga atgtcttcca aacgtggaaa ctctcgtaaa caaagaggtt ttagaggctt
    17101 atttaccatg gactaaagtt gtacaagaaa agtgcaaata agaaatctcc agattaggaa
    17161 ctatccgtga gttctctagt ctggagattt ttcaatatac ttcgttattg gacggttaca
    17221 attcataaag tgctgtagag agataacgtt caccgttatc tggtgctagg gcaaggactt
    17281 ttttacctgt acctaatttt ttggcaacct cgatggctcc gtagatagct gcagctgagg
    17341 aaatccctac aaggaagcct tcttttccac caatttcacg tccgagtgca agagcgtcat
    17401 ctgatgttac acgaacgata ccatcatagg ctttagtatc aagtgtatca ggaataaatc
    17461 cagctgagat accttgaatt ttgtgaggac caggtttttc accagataga atagcagatt
    17521 catctgcttc tactgcaaaa acttgaatgt tagaattttc tgatttgagt gcatgagaaa
    17581 caccagaaat cgttccaccg gtacctactc cagcaacaaa ggcatctaat ccatctttac
    17641 cgaaagcagc tagtatctca gctcctgttg ttctttcgtg tacttctgga ttagctggat
    17701 tgtcaaattg aagaggaagg aaaccatcac gttcagcagc gatttcttga gccttagcaa
    17761 tagcaccttt cattccctcg ctaccaggag ttaggacgag ttcagcacca taagcttgga
    17821 taattttacg tcgttctaca ctcatagttt caggcataac gatgacgact ttataccctt
    17881 tagcagcacc tacccatgaa agtccaatac cagtgtttcc acttgttgct tcaacaatag
    17941 tagaaccagg tttcagaata ccatcttgtt cagctttttc aatcatgcta agggcaatac
    18001 ggtcttttac agatgaacca ggattaaatg cttcaagctt tatatagacg tctgcagcac
    18061 cttctggcac gatgttgtta agtttaacaa tcggtgtttg accgattaat tcagtaatgt
    18121 tgttataaat agacatatga atacctcttt gtataagata tctttagtat aacgctatcc
    18181 ataccatttg taaaatatag aaatcctatc gaagcgatag gatttctata tatcatagat
    18241 caaagtcatt aattttcaag tgattgcgtt cagctaattc tagtaaatag gtgtgaagga
    18301 aaacgatatc aggtttcgtt tgaattgctt cattttctag tatattttgg tacgcatctt
    18361 tttattttgc tcgctaaagt agttgtgaga atgtcaacga aacgctgtat tattcattgt
    18421 ttacactaag tttacaggaa cttcaacctc tcgtaaacct tggtcagtta aagtgacttt
    18481 tccattaaaa aactccacaa gtgcagcttt aatagtttct ttttcttctt tatcaacata
    18541 aatcatcgta tcgacttgat ctgtaaagtt tgtatccagc tccatgagat catgttcttt
    18601 aaggaagtta ctgtactctt ggtactgagc ataagacatt tgaatagcaa tgccagcctg
    18661 ttcttttatt tcaataatac caatttcttt gacagctaag gcgacactgc cggcgtaagc
    18721 acgaattagt cctccagcgc ctagtttaat accaccaaag tagcgtgtca cgaccacaca
    18781 gacattggtg agattgtgat tttctagtac cccaagcatg ggaacaccag cagtaccact
    18841 aggctcacca tcatcacttg tacgtttaat ttcactacgt tctccaataa taaaggcaga
    18901 gcagttatgt gtcgctttgt agtgttcttt tttgatggca gtaatgaagt cacgagcctc
    18961 ttcttcgcta taaacacgct tggcatggca gataaagcga gattttttga tttcttcttg
    19021 gacttggtcg tcctctttaa ttgttctaaa ttccatgatt taattgtact aaaaaatcat
    19081 ctaaagtgct agtttttacg aataaagaag tatgaaagta aatttagatt atctcggtcg
    19141 tttatttact gagaatgaat taacagaaga agaacgtcag ttggcggaga aacttccagc
    19201 aatgagaaag gagaagggga aacttttctg tcaacgttgt aatagtacta ttctagaaga
    19261 atggtatttg cccatcggtg cttactattg tcgagagtgc ttgctgatga agcgagtcag
    19321 aagtgatcaa actttatact attttccgca ggaggatttt ccgaagcaag atgttctcaa
    19381 atggcgcggc caattaactc cttttcaaga gaaggtgtca gagggactgc ttcaagcagt
    19441 agacaagcaa aagccaacct tagttcatgc ggtaacagga gctggaaaga cagaaatgat
    19501 ttatcaagta gtggctaaag tgatcaatgc gggtggtgca gtgtgtttgg ctagtcctcg
    19561 catagatgtt tgtttggagc tgtacaagcg cctgcaacag gatttttctt gcgggatagc
    19621 tttgctacat ggagaatcgg aaccttattt tcgaacacca ctagttgttg caacaaccca
    19681 tcagttattg aagttttatc aagcttttga tttgctgata gtggatgaag tagatgcttt
    19741 tccttatgtt gataatccaa tgctttacca cgctgtcaag aatagtgtaa aggagaatgg
    19801 attgagaatc tttttaacag cgacttcgac caatgagtta gataaaaagg tccgtttagg
    19861 agaactaaaa agactgaatt taccgagacg gtttcatgga aatccgttga ttattccaaa
    19921 accaatttgg ttatcggatt ttaatcgcta cttagacaag aatcgtttgt caccaaagtt
    19981 aaagtcctat attgagaagc agagaaagac agcttatccg ttactcattt ttgcttcaga
    20041 aattaagaaa ggggagcagt tagaagaaat cttacaggag caatttccaa atgagaaaat
    20101 tggctttgta tcttctgtaa cagaggatcg attagagcaa gtacaagctt ttcgagatgg
    20161 agaactgaca atacttatca gtacgacaat cttggagcgt ggagttacct tcccttgtgt
    20221 ggatgttttc gtagtagagg ccaatcatcg tttgtttacc aagtctagtt tgattcagat
    20281 tggtggacga gttggacgaa gcatggatag accgacagga gatttgcttt tcttccatga
    20341 tgggttaaat gcttcaatca agaaggcgat taaggaaatt cagatgatga ataaggaggc
    20401 tggtctatga agtgcttgtt atgtgggcag actatgaaga ctgttttaac ttttagtagt
    20461 ctcttacttc tgaggaatga tgactcttgt ctttgttcag actgtgattc tacttttgaa
    20521 agaattgggg aagagaactg tccaaattgt atgaaaacag agttgtcaac aaagtgtcaa
    20581 gattgtcaac tttggtgtaa agaaggagtt ggagtcagtc atagagcgat ttttacttac
    20641 aatcaagcta tgaaggattt tttcagtcgg tataagtttg atggagactt cctgttaaga
    20701 aaagttttcg cttcattttt aagtgaggag ttgaaaaagt acaaagagta tcaatttgtt
    20761 gtaattcccc taagtcctga tagatatgct aatagaggat ttaatcaggt tgagggcttg
    20821 gtagaggcag caggttttga gtatctggat ttattagaga aaagagaaga gagagccagt
    20881 tcttctaaaa atcgttcaga gcgcttgggg acagaacttc ctttctttat taaaagtgga
    20941 gtcactattc ctaaaaaaat cctacttata gatgatatct atactacagg agcaactata
    21001 aatcgtgtta agaaactgtt ggaagaagct ggtgctaagg atgtaaaaac attttccctt
    21061 gtaagatgag gaaaaaattt gcaaaaatag aatgaaagcg ttataataaa atcataaaaa
    21121 caaaaatgtt tagaaagaag gtactcatat gattaaatat agtatccgtg gtgaaaacct
    21181 agaagtaaca gaagcaattc gtgattatgt agtttctaaa ctcgaaaaga tcgaaaagta
    21241 cttccaacca gaacaagagt tggatgcccg aattaactta aaagtttatc gtgaaaaaac
    21301 ggctaaagtg gaagtaacga ttccgcttgg atctattact ctccgcgcag aagatgtatc
    21361 tcaagatatg tatggttcaa ttgaccttgt aactgataaa attgaacgtc agattcgtaa
    21421 aaataaaaca aaaatcgagc gtaaaaataa aaataaggta gcaactggtc aattatttac
    21481 agatgctttg gtggaagatt caaatgttgt ccagtctaaa gttgttcgtt caaaacaaat
    21541 tgatttaaaa ccaatggatt tggaagaagc aattctacaa atggatttat tggggcatga
    21601 tttctttatc tatgtggatg ttgaagatca gacaaccaat gtgatttatc gtcgtgagga
    21661 tggcgaaatt ggtttgttag aggttaaaga atcttaattt caatatgtgt aaaggtaggt
    21721 ttactgaatt gtaaactacc tttttcttat aattgataga attactgatt acacttttaa
    21781 aaagtcgctt tttgaggggt attatggtat aatgggtgcc aagaggtttt gaatgaaaaa
    21841 attttatgta agtccaattt ttcctattct agtaggattg attgcgtttg gagtcttatc
    21901 cactttcatt atttttgtta ataataatct gttgacggtt ttaattttgt ttctttttgt
    21961 aggaggctat gtttttttat ttaagaaact gagagtgcat tatacaagga gtgatgtaga
    22021 acagatacag tatgtaaacc accaagcgga agaaagtttg acatctctat tggaacagat
    22081 gcctgtaggt gttatgaaat tgaatttatc ttctggagag gttgagtggt ttaatcccta
    22141 tgctgaattg attttgacca aggaagatgg tgattttgat ttagaagctg ttcaaacgat
    22201 tattaaggct tcagtaggaa atccgtctac ttatgccaag cttggtgaga agcgttatgc
    22261 tgttcatatg gatgcttctt ccggtgtttt gtattttgta gatgtatcca gggaacaagc
    22321 cataacagat gaattggtaa caagtcgacc agtgattggg attgtctctg tggataatta
    22381 tgatgatttg gaggatgcaa cttctgagtc agatattagt caaatcaata gttttgtagc
    22441 taattttata tcagagtttt cagaaaaata catgatgttt tctcgtcggg taagtatgga
    22501 tcgattttat ctatttactg actacacggt gcttgagggc ttgatgaatg ataaattttc
    22561 tgttattgat gctttcagag aagagtcgaa acagagacag ttgcccttga ccttaagtat
    22621 gggattttct tatggcgatg gaaatcatga tgagataggg aaagttgctt tgctcaattt
    22681 gaacttggct gaagtacgtg gtggcgacca ggtggttgtt aaggaaaacg acgaaacgaa
    22741 aaatccagtt tattttggtg gtgggtctgc tgcttcaatc aagcgtacac ggactcgtac
    22801 gcgcgctatg atgacagcta tttcagataa gattcggagt gtagatcagg tttttgtagt
    22861 cggtcacaaa aatttagaca tggatgcttt gggctctgct gtaggtatgc agttgttcgc
    22921 cagcaatgtg attgaaaata gctatgctct ttatgatgaa gaacaaatgt ctccagatat
    22981 tgaacgagct gtttcattca tagaaaaaga aggagttacg aagttgttgt ctgttaagga
    23041 tgcaatgggg atggtgacca atcgttcttt gttgattctt gtagaccatt caaagacagc
    23101 cttaacatta tcaaaagaat tttatgattt atttacccaa accattgtta ttgaccacca
    23161 tagaagggat caggattttc cagataatgc ggtcattact tatatcgaaa gtggtgcaag
    23221 tagtgccagt gagttggtaa cggaattgat tcagttccag aattctaaga aaaatcgttt
    23281 gagtcgtatg caagcaagtg ttttgatggc tggtatgatg ttggatacta aaaatttcac
    23341 ctcgcgagta actagtcgga catttgatgt tgctagctat ctcagaacgc gcggaagtga
    23401 tagtattgct atccaggaaa tcgctgcgac agattttgaa gaatatcgtg aggtcaatga
    23461 acttatttta caggggcgta aattaggttc agatgtacta atagcagagg ctaaggacac
    23521 gaaatgctat gatacagttg ttattagtaa ggcagcagat gccatgttag ccatgtcagg
    23581 tattgaagcg agttttgttc ttgcgaagaa tacacaagga tttatctcta tctcagctcg
    23641 aagtcgtagt aaactgaatg tacaacggat tatggaagag ttaggcggtg gaggccactt
    23701 taatttggca gcagctcaaa ttaaagatgt aaccttgtca aaagcaggtg aaaaactgac
    23761 agaaattgta ttaaatgaaa taaaggaaaa ggagaaagaa gaatgaaagt aatcttttta
    23821 gcagatgtta aaggaaaagg taaaaaaggc gaaattaagg aagtaccaac agggtatgcg
    23881 caaaactttc ttatcaaaaa gaatctagcc aaagaagcga ctgctcaagc tgtaggtgaa
    23941 cttcgtggta aacaaaaatc ggaagaaaaa gctcacgctg agatgattgc agaaggaaaa
    24001 gcaattaaag cacaacttga agcagaagaa actgttgtag aatttgttga aaaagttggt
    24061 ccagatggtc gtacctttgg ttctattacc aataagaaga ttgcagaaga attgcaaaag
    24121 caatttggaa ttaagattga taaacgtcat attcaagtac aagctccgat tcgagcggtt
    24181 ggtttgattg atgtgccagt gaaaatctat caagatatca caagtgtaat caatcttcgt
    24241 gtgaaagaag gataagttta caccttcttg acaagattgt aaaaggaagg gaagtctgat
    24301 ggcagaagta gaagagttac gagtacaacc tcaagatatc ttagctgagc aatccgtttt
    24361 aggggctatc tttattgatg agagtaaact tgtttttgtg cgagaataca ttgagtctcg
    24421 ggactttttt aagtatgccc atcgtttgat tttccaagcc atggtcgatt tatccgatcg
    24481 tggtgatgcc atagatgcaa caacggttcg tactatcctt gataatcaag gtgatttaca
    24541 gaatattggt ggcttgtctt acttggttga gattgtcaat tctgtgccaa cttctgctaa
    24601 tgcggagtat tatgctaaga ttgttgcaga aaaagcaatg ctacgtcgtt taattgccaa
    24661 gttgacagag tctgtcaacc aagcttacga agcgtcacaa ccagctgatg aaattattgc
    24721 tcaggcagaa aaagggctga ttgatgtcag tgaaaatgca aatcgaagcg ggtttaagaa
    24781 cattcgagat gtgttgaatc tcaactttgg aaatctggaa gctcgctcgc aacaaacgac
    24841 cgatattaca ggtattgcga caggttatcg tgatttggat catatgacaa caggacttca
    24901 tgaggaggag ttgattatct tagcagctcg tccagcagtt ggtaagacag catttgcctt
    24961 gaatatcgct cagaacattg ggactaagtt ggacaaaacg gttgctattt tttcactcga
    25021 aatgggtgcg gaaagcttgg tagatcgtat gttagctgca gaaggcttag tggagtcaca
    25081 ttctatccgt acagggcaat tgacagatga ggagtggcaa aaatatacta ttgctcaggg
    25141 taatctagct aacgccagta tctatatcga tgatacgcca ggtattcgga ttacagagat
    25201 tcgttctcgt tctcgtaaat tggctcaaga aactggaaat cttggtttga ttttgataga
    25261 ctatttgcaa cttatcacgg gaactggtcg agaaaatcgt caacaagaag tttctgaaat
    25321 ttctcgtcag ttgaaaatac tagccaagga attgaaggtt ccagtaatcg ctctgagtca
    25381 gctttctcgt ggtgtagaac aacgtcagga caagagaccg gtcttgtctg atattcgtga
    25441 atctgggtct attgagcagg acgctgatat cgtagctttt ctctatcgcg atgactacta
    25501 tgaacgtggt ggtgaagaag aggaaggtat cccaaataat aaggtggaag ttattatcga
    25561 gaaaaaccgt agtggagctc gtggaacagt ggaattgatt ttccaaaaag aatacaataa
    25621 attttcaagt atctcaaaga gggaggcata agatgtcaga tgcatttaca gatgtagcca
    25681 agatgaaaaa aatcaaagaa gaaatcaagg cacatgaggg acaagtcgta gaaatgactt
    25741 tggagaatgg tcgtaagcgc caaaaaaata gattgggtaa gctaattgaa gtttatccat
    25801 ccctatttat tgtggagttt ggggatgtgg aaggagataa acaagttaat gtttacgttg
    25861 aatcctttac ttactcagat attcttacag aaaagaattt gattcattat cttgactaaa
    25921 gtgagaaatt ttctcacttt ttcttttttc tccgaataat ttaggtgaag gcaatcatcg
    25981 ctttatatta tttttcaagg aggaagaatg aaaattttac cgtttatagc aagaggaaca
    26041 agttattact tgaagatgtc agttaaaaag cttgttcctt ttttagtagt aggattgatg
    26101 ctagcagctg gtgatagtgt ctatgcctat tccagaggaa atggatcgat tgcgcgtggg
    26161 gatgattatc ctgcttatta taaaaatggg agccaggaga ttgatcagtg gcgcatgtat
    26221 tctcgtcagt gtacttcttt tgtagccttt cgtttgagta atgtcaatgg ttttgaaatg
    26281 ccggcagctt atggaaatgc gaatgaatgg ggacatcgtg ctcgtcggga aggttatcgt
    26341 gtagataata caccgacgat tggttccatt acttggtcta ctgcaggaac ttatggtcat
    26401 gttgcctggg tgtcaaatgt aatgggagat cagattgaga ttgaggaata taactatggt
    26461 tatacagaat cctataataa acgaattata aaagcaaaca cgatgacagg atttattcat
    26521 tttaaagatt tggatggtgg cagtgttggg aatagtcaat cctcaacttc aacaggcgga
    26581 actcattatt ttaagaccaa gtctgctatt aaaactgaac ccctagttag tgcaactgtg
    26641 attgattact attatcctgg agagaaggtt cattatgatc aaattctcga aaaagacggc
    26701 tacaagtggt tgagttatac tgcctataat ggaagctatc gttatgttca attggaggct
    26761 gtgaataaaa atcctctagg tcattctgtt ctttcttcaa caggtggaac tcattatttt
    26821 aagaccaagt ctgctatcaa aactgaaccc ctagttagtg caactgtgat tgattactat
    26881 tatcctgggg agaaggttca ttatgatcag atacttgaaa aagacggcta caagtggttg
    26941 agttatacgg cttataacgg aagtcgtcgc tatatacagc tagagggagt gacttcttca
    27001 caaaattatc agaatcaatc aggaaatatc tctagctatg gatccaataa tagttcaact
    27061 gtcggttgga agaaaataaa tggtagttgg tatcatttca aatcaaatgg ttataaatca
    27121 acaggatggc tgaaagatgg ttctagctgg tattatttga aattatctgg tgaaatgcag
    27181 acaggatggt taaaggagaa tggctcgtgg tattatctgg gtagttcagg ggcaatgaaa
    27241 acaggctggt accaggtctc tggtaagtgg tattattctt actcttcagg cgccttagct
    27301 gttaatacga cggtggatgg ctacagagta aacagtgatg gagaacgagt atagaaaatt
    27361 ggagtaggag aaatttcctg ctcttttctg taagcagttt ccttgacatt tttcttattt
    27421 tgcgctatca tatatacata taatatatgg gagtctgtgt acagtctgag aggaagtgtt
    27481 aaacttcgac cgcacctgat ctgggtaatg ccagcggagg gaacgatact tagtctaatt
    27541 ttgcaccttt tccatgtatg gtaaaggttt ttcttttttt aaaaaggaaa acgagaagag
    27601 gaggttctta tgaaagcaag cattgccttg caagttttac ccctagcaca ggggattgat
    27661 cggatagctg ttattgatca ggtcattgct tatctgcaaa ctcaagaagt gacgatggta
    27721 gtgacaccat ttgaaacggt cttggaaggg gagtttgatg agcttatgcg cattctaaaa
    27781 gaagcgctgg aagtggcagg gcagggggca gacaatgtct ttgccaatgt caaaataaat
    27841 gtaggagaga ttttaagtat tgatgagaaa cttgagaagt atactgagac gacacattag
    27901 tctattgggc tttctcggag tcttgtcaat ctggcagtta gcaggttttc ttaaacttct
    27961 ccccaagttt atcctgccga cacctcttga aattctccag ccctttgttc gtgacagaga
    28021 atttctctgg caccatagct gggcgacctt gagagtggct ttactggggc tgattttggg
    28081 agttttgatt gcctgtctta tggctgtact catggatagt ttgacttggc tcaatgacct
    28141 gatttaccct atgatggtgg tcattcagac cattccgacc attgccatag ctcctatcct
    28201 ggtcttgtgg ctaggttatg ggattttgcc caagattgtc ttgattatct taacgacaac
    28261 ctttcccatc atcgtcagca ttttggacgg ttttaggcat tgcgacaagg atatgctgac
    28321 cttgtttagt ctgatgcggg ccaagccttg gcaaatcctg tggcatttta aaattccagt
    28381 cagcctgccc tacttttatg ctggtctgag ggtcagtgtc tcctacgctt ttatcacaac
    28441 agtggtatct gagtggttgg gaggctttga ggggcttggt gtttatatga ttcagtccaa
    28501 gaaattgttt cagtatgata ccatgttcgc tattattatt ctggtatcga ttatcagcct
    28561 tttgggtatg aagttggtcg atattagtga aaaatatgtt attaaatgga aacgttcgta
    28621 gaattagaat gtttctgaaa aagaaaagag gaaatcaaaa tgaagaaaac atggaaagtg
    28681 tttttaacgc ttgtaacagc tcttgtagct gttgtgcttg tggcctgtgg tcaaggaact
    28741 gcttctaaag acaacaaaga ggcagaactt aagaaggttg actttatcct agactggaca
    28801 ccaaatacca accacacagg gctttatgtt gccaaggaaa aaggttattt caaagaagct
    28861 ggagtggatg ttgatttgaa attgccacca gaagaaagtt cttctgactt ggttatcaac
    28921 ggaaaggcac catttgcagt gtatttccaa gactacatgg ctaagaaatt ggaaaaagga
    28981 gcaggaatca ctgccgttgc agctattgtt gaacacaata catcaggaat catctctcgt
    29041 aaatctgata atgtaagcag tccaaaagac ttggttggta agaaatatgg gacatggaat
    29101 gacccaactg aacttgctat gttgaaaacc ttggtagaat ctcaaggtgg agactttgag
    29161 aaggttgaaa aagtaccaaa taacgactca aactcaatca caccgattgc caatggggtc
    29221 tttgatactg cttggattta ctacggttgg gatggtatcc ttgctaaatc tcaaggtgta
    29281 gatgctaact tcatgtactt gaaagactat gtcaaggagt ttgactacta ttcaccagtt
    29341 atcatcgcaa acaacgacta tctgaaagat aacaaagaag aagctcgcaa agtcatccaa
    29401 gccatcaaaa aaggctacca atatgccatg gaacatccag aagaagctgc agatattctc
    29461 atcaagaatg cacctgaact caaggaaaaa cgtgactttg tcatcgaatc tcaaaaatac
    29521 ttgtcaaaag aatacgcaag cgacaaggaa aaatggggtc aatttgacgc agctcgctgg
    29581 aatgctttct acaaatggga taaagaaaat ggtatcctta aagaagactt gacagacaaa
    29641 ggcttcacca acgaatttgt gaaataatga cagaaattag actagagcac gtcagttatg
    29701 cctatggtca ggagcggatt ttagaggata tcaacctaca ggtgacttca ggcgaagtgg
    29761 tttccatcct aggcccaagt ggtgttggaa agaccaccct ctttaatcta atcgctggga
    29821 ttttagaagt tcagtcaggg agaattgtcc ttgatggtga agaaaatccc aaggggcgcg
    29881 tgagttatat gttgcaaaag gatctgctct tggagcacaa gacggtgctt ggaaatatca
    29941 ttctgcccct cttgattcaa aaggtggata aggcagaagc tatttcccga gcggataaaa
    30001 ttcttgcgac cttccagctg acagctgtaa gagacaagta tcctcatgaa cttagcggtg
    30061 ggatgcgcca gcgtgtagcc ttactccgga cctacctttt tgggcacaag ctctttctct
    30121 tagatgaggc ctttagcgcc ttggatgaga tgacaaagat ggaactccac gcttggtatc
    30181 ttgagattca caagcagttg cagctaacaa ccctgatcat cacgcatagt attgaggagg
    30241 ccctcaatct cagcgaccgc atctatatct tgaaaaatcg ccctgggcag attgtttcag
    30301 aaattaaact agattggtct gaagatgagg acaaggaagt ccaaaagatt gcctacaaac
    30361 gtcaaatctt ggcagaatta ggcttagata agtagaaaaa tagggagttg gtgaagatta
    30421 tcctttacca gcgccctttt tcttttaaaa atgagaaaat ttcggtataa tagtcaaaca
    30481 aggtcaaggt ttaaagagag aggtgggttt gttatgagat ttaaaaatac atcggatcat
    30541 attgaggcct acatcaaggc gattttagat caatctggta tcgtggagtt gcaacggagt
    30601 cagttggcag atacctttca ggttgttcct agtcagatta actacgtgat caagacacgc
    30661 tttacggaaa gtagaggcta cttggttgaa agtaagcgtg gtggcggagg ctacattcgt
    30721 ataggacgga ttgagttttc tagtcatcat gaaatgctcc gggagctgct ttactcgatt
    30781 ggtgagcgag tcagtcaaga aatttatgag gatattctcc agcttttggt tgagcaggaa
    30841 ttgatgacca agcaggagat gaatttgcta gaatcagtag ctttggatcg cgttttagga
    30901 gaagaagctc cagttgttcg agcaaacatg ctacgtcagg tcatacaaga ggtagataga
    30961 aaagggaagt aagatgaact attcaaaagc attgaatgaa tgtatcgaaa gtgcctacat
    31021 ggttgctgga cattttggag ctcgttatct agagtcgtgg cacttgttga ttgccatgtc
    31081 taatcacagt tatagtgtag caggggcaac tttaaatgat tatccgtatg agatggaccg
    31141 tttagaagag gtggctttgg aactgactga aacggactat agccaggatg aaacctttac
    31201 ggaattgccg ttctcccgtc gtttgcaggt tctttttgac gaagcagagt atgtagcgtc
    31261 agtggtccat gctaaggtgc tagggacaga gcacgtcctc tatgcgattt tgcatgatgg
    31321 caatgccttg gcgactcgta tcttggagag ggctggtttt tcttatgaag acaagaaaga
    31381 tcaggtcaag attgctgctc ttcgtcgaaa tttagaagaa cgggcaggct ggactcgtga
    31441 agatctcaag gctttacgcc aacgccatcg tacagtagct gacaagcaaa attctatggc
    31501 caatatgatg ggcatgccgc agactcctag tggtggtctc gaggactata cgcatgattt
    31561 gacagagcaa gcgcgttctg gcaagttaga accagtcatc ggtcgggaca aggaaatctc
    31621 acgtatgatt caaattttga gccgtaagac taagaacaac cctgtcttgg ttggggatgc
    31681 tggtgtcggg aaaacagctc tggcccttgg tcttgcccag cgtattgcta gtggtgacgt
    31741 gcctgcggaa atggctaaga tgcgcgtgtt agaacttgat ttgatgaatg tcgttgcagg
    31801 gacacgcttc cgtggtgact ttgaagaacg catgaataat atcatcaagg atattgaaga
    31861 agatggccaa gtcatcctct ttatcgatga actccacacc atcatgggtt ctggtagcgg
    31921 gattgattcg actctggatg cggccaatat cttgaaacca gccttggcgc gtggaacttt
    31981 gagaacggtt ggtgccacca ctcaggaaga atatcaaaaa catatcgaaa aagatgcggc
    32041 tctttctcgt cgtttcgcta aagtgacgat tgaagaacca agtgtggcag atagtatgac
    32101 tattttacaa ggtttgaagg cgacttatga gaaacatcac cgtgtacaaa tcacagatga
    32161 agcggttgaa acagcggtta agatggctca tcgttattta accagtcgtc acttgccaga
    32221 ctctgctatc gacctcttgg acgaagcagc agcaacagtg caaaataagg caaagcatgt
    32281 aaaagcagac gattcagatt tgagtccagc tgacaaggcc ctgatggatg gcaagtggaa
    32341 acaggcagcc cagctaatcg caaaagaaga ggaagtacct gtctacaaag acttggtgac
    32401 agagtctgat attttgacca ccttgagtcg cttgtcagga atcccagttc aaaaactgac
    32461 tcaaacggat gctaagaagt atttaaatct tgaagcagaa ctccataaac gtgtcatcgg
    32521 tcaagatcaa gctgtttcaa gtattagtcg tgccattcgc cgtaatcagt caggaattcg
    32581 cagtcacaag cgtccgatcg gttcctttat gttcctagga cccacaggtg tcggtaagac
    32641 cgagttggcc aaggctttgg cagaagttct ctttgatgac gaatcagccc ttatccgctt
    32701 tgatatgagt gagtatatgg agaaattcgc agccagccgt ctaaatggag ctcctccagg
    32761 ctatgtgggt tacgaagaag gtggggagtt gacagagaag gttcgcaata aaccctattc
    32821 cgttctcctc tttgatgagg tagagaaggc tcacccagat atctttaatg ttctcttgca
    32881 ggttctggat gacggtgtct tgacagatag caagggacgc aaggtcgatt tttcaaatac
    32941 cattatcatt atgacatcga acctaggtgc gactgccctt cgtgatgata agactgttgg
    33001 ttttggggct aaggatattc gttttgacca ggaaaatatg gaaaaacgca tgtttgaaga
    33061 actgaaaaaa gcttatagac cggaattcat caaccgtatt gatgagaagg tggtcttcca
    33121 tagcctatct agtgaccata tgcaggaagt ggtgaagatt atggtcaagc ctttagtggc
    33181 aagtttggct gaaaaaggca ttgacttgaa attacaagct tcagctctga aattgttagc
    33241 aaatcaagga tatgacccag agatgggagc tcgcccactt cgcagaaccc tgcaaacaga
    33301 agtggaggac aagttggcag aacttcttct taagggagat ttagtggcag gcagcacact
    33361 taagattggt gtcaaagcag gccagttaaa atttgatatt gcataaaaga ataaaagtat
    33421 cagcatctga ccataagtca cagtggagtg aaactcaatg aaaatcaaag agcaaactag
    33481 gcagctagcc gcaggttgct ttgaggttgt agatagagct gacgtggttt gaagagattt
    33541 tcgaagagta taagctgcaa gatgaatgat tttcttgtat tgacgttgtt gttgacaaaa
    33601 agtagcggat aaatgaaatc cattccatta tcatagatga tagactggta ggaaattttc
    33661 aaatagcata caggaaatag atgtatggag ttctggtagt agaaagggag agagatgaac
    33721 attttagttg cagatgacga ggaaatgatt agagaaggaa ttgcagcatt tctgacagaa
    33781 gagggttatc atgtcattat ggctaaggat ggacaagagg tcttggaaaa atttcaagat
    33841 ctccctatcc atctcatggt actggattta atgatgccta ggaagagtgg ttttgaagtg
    33901 ttaaaagaaa tcaatcaaaa gcacgatatt cctgtcatcg tcttgagtgc tctgggagat
    33961 gaaactactc agtcacaggt atttgatctc tatgctgatg atcatgtgac aaaacctttt
    34021 tctttggtac tgcttgtcaa gcgtattaag gcgcttatca gacgttacta cgtcatagag
    34081 gatatttggc gatatcagga tgtaacagtg gattttacct cttacaaagc acattataaa
    34141 aatgaagaaa ttgatctcaa accaaaggaa gtactggtac taaagtgttt gattcagcat
    34201 aaaaatcaag ttttaagtag agagcagata ttggaagaaa tttcaaaaga tgtagctgat
    34261 ttaccttgtg atagggtcgt tgatgtctat attcgtactc ttcgcaaaaa attagcttta
    34321 gattgtatcg tgactgtgaa aaatgttggg tataagatta gcttatgata aaaaatccta
    34381 aattattaac caagtctttt ttaagaagtt ttgcaattct aggtggtgtt ggtctagtca
    34441 ttcatatagc tatttatttg acctttcctt tttattatat tcaactggag ggggaaaagt
    34501 ttaatgagag cgcaagagtg tttacggagt atttaaagac taagacatct gatgaaattc
    34561 caagcttact ccagtcttat tcaaagtcct tgaccatatc tgctcacctt aaaagagata
    34621 ttgtagataa gcgactccct cttgtgcatg acttggatat taaagatgga aagctatcaa
    34681 attatatcgt gatgttagat atgtctgtta gtacagcaga tggtaaacag gtaacagtgc
    34741 aatttgttca cggggtggat gtctacaaag aagcaaagaa tattttgctt ttgtatctcc
    34801 catatacatt tttggttaca attgcttttt cctttgtttt ttcttatttt tatactaaac
    34861 gcttgctcaa tcctcttttt tacatttcag aagtgactag taaaatgcaa gatttggatg
    34921 acaatattcg ttttgatgaa agtaggaaag atgaagttgg tgaagttggt gaagttggaa
    34981 aacagattaa tggtatgtat gagcacttgt tgaaggttat tcatgagttg gaaagtcgta
    35041 atgagcaaat tgtaaaattg caaaatcaaa aggtttcctt tgtccgcgga gcatcacatg
    35101 agttgaaaac ccctttagcc agtcttagaa ttatcctaga gaatatgcag cataatattg
    35161 gagattacaa agatcatcca aaatatattg caaagagtat aaataagatt gaccagatga
    35221 gccacttatt ggaagaagta ctggagtctt ctaaattcca agagtggaca gagtgtcgtg
    35281 agaccttgac tgttaagcca gttttagtag atattttatc acgttatcaa gaattagctc
    35341 attcaatagg tgttacaatt gaaaatcaat tgacagatgc taccagggtc gtcatgagtc
    35401 ttagggcatt ggataaggtt ttgacaaacc tgattagtaa tgcaattaaa tattcagata
    35461 aaaatgggcg cgtaatcata tccgagcaag atggctatct ctctatcaaa aatacatgtg
    35521 cgcctctaag tgaccaagaa ctagaacatt tatttgatat attctatcat tctcaaatcg
    35581 tgacagataa ggatgaaagt tccggtttgg gtctttacat tgtgaataat attttagaaa
    35641 gctatcaaat ggattatagt tttctccctt atgaacacgg tatggaattt aagattagct
    35701 tgtagacaga ttagtttttt attaaagttc atatagggtt aacataagtg tgttattctt
    35761 tgtgtagata aaagaaagga tactaatatg gtattagcga ttattttagt aacattcctt
    35821 attcgattga tttttttaaa gcgttcgata gagaatgaga aacgaatcct tagcaatggc
    35881 gggaaagaat ttggagttga gaatacaaaa cgattaacta tggctcatat tgttttttat
    35941 ctctcttgct tggttgaggc aatggtgcac aagacaattt ttgatggcat gggcatggtt
    36001 ggtttagtct tgcttatttt ttctatgctg atgttgatgt tggtgattca cttgttggga
    36061 gatatttgga cagtgaagct tatgcttgtc aataatcaca aatatgtaga tcatatcttg
    36121 tttaggacag taaaacaccc taattacttt ttaaatattt tacctgagtt gattggcttg
    36181 accttgttga gtcatgccta tatgactttt gttttagttt ttccagttta tgcagttatt
    36241 ttgtatcgac gaatagctga agaggaaaag ctattacatg aagttataat cccaaatgga
    36301 agcataaaga gataaataca aaattcgatt tatatacagt tcatattgaa gtgatatagt
    36361 aaggttaaag aaaaaatata gaaggaaata aacatgtttg catcaaaaag cgaaagaaaa
    36421 gtacattatt caattcgtaa atttagtatt ggagtagcta gtgtagcagt tgccagcttg
    36481 ttcttaggag gagtagtcca tgcagaaggg gttggtggta ggaatacctc cacggttaca
    36541 tctagtgggc aagatacatc gaagaagtat gctgatgaag tcaagtcgca ttatcaaagt
    36601 atattggaga aggtcagaaa aagtttggaa aaagatcgac atacccaaaa tgtcggctta
    36661 atcacaaagt tgagcgaaat taaaaagaag tatttgtatg aattagaagt taatgtttta
    36721 ttagaagaga agtcgaaagc tgagttgccg tcaaaagcta aagcagagtt agacgcagct
    36781 tttgagcagt ttaaaaaaga accagaactt actaaaaaag tagcagaagc tcagaagaag
    36841 gttgaagaag ctaagaaaaa agccgaggat caaaaagaag aagatttccg taactaccca
    36901 accaatactt acaaaacaat cgaacttgaa attgctgagt ccgatgtgaa agttaaagaa
    36961 gcggagcttg aactattgaa agaggaagct aaggaacatc gagacgaggg cacaattaag
    37021 caagtagaag agaaagttaa gagtgaaaaa gctgaggcta caaggttaga agaaatcaag
    37081 acagaacgta aaaaagcaga agaagaagct aaacgaagag cagatgctaa agagcaaggt
    37141 aaaccaaagg ggcgggcaaa acgaggagtt cctggagagc aagcaacacc tgataaaaaa
    37201 gaaaatgatg cgaagtcttc agattctagc gtaggtgaag aaactcttcc aaacccatcc
    37261 ctgaaaccag aaaaaaaggt agcagaagct cagaagaagg ttgaagaagc taagaaaaaa
    37321 gccaaggatc aaaaagaaga agatcaccgt aactacccaa ccattactta caaaacgctt
    37381 gaacttgaaa ttgctgagtc cgatgtggaa gttaaaaaag cggagcttga actagtaaaa
    37441 gaggaagcta agggatctcg aaacgaggaa aaagttaagc aagcaaaagc ggaagttgag
    37501 agtaaaaaag ctgaggctac aaggttagaa aaaatcaaga cagatcgtaa aaaagcagaa
    37561 gaagctaaac gaaaagcagc agaagaagat aaagttaaag aaaaaccagc tgaacaacca
    37621 caaccagcgc cggctcctca accagaaaaa ccagctccag ctccaaaacc agagaatcca
    37681 gctgaacaac caaaagcaga aaaaccagct gatcaacaag ctgaagaaga ctatgctcgt
    37741 agatcagaag aagaatataa tcgcttgact caacagcaac cgccaaaaac tgaaaaacca
    37801 gcacaaccat ctactccaaa aacaggctgg aaacaagaaa acggtatgtg gtacttctac
    37861 aatactgatg gttcaatggc gacaggatgg ctccaaaaca atgg
//
LOCUS       DAVKJJ010000017        36633 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000017 DAVKJJ010000000
VERSION     DAVKJJ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36633
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.017"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            187..684
                     /locus_tag="VW144_000837"
     CDS             187..684
                     /locus_tag="VW144_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU9600308.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTAWDFTM
                     FEEKS"
     gene            684..2339
                     /gene="dnaX"
                     /locus_tag="VW144_000838"
     CDS             684..2339
                     /gene="dnaX"
                     /locus_tag="VW144_000838"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU9600309.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVNLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNAGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2367..2561
                     /locus_tag="VW144_000839"
     CDS             2367..2561
                     /locus_tag="VW144_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEU9600310.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2711..3481
                     /gene="sufC"
                     /locus_tag="VW144_000840"
     CDS             2711..3481
                     /gene="sufC"
                     /locus_tag="VW144_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU9600311.1"
                     /translation="MSVIEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3509..4771
                     /gene="sufD"
                     /locus_tag="VW144_000841"
     CDS             3509..4771
                     /gene="sufD"
                     /locus_tag="VW144_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU9600312.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKVTANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKDAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4782..6008
                     /locus_tag="VW144_000842"
     CDS             4782..6008
                     /locus_tag="VW144_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9600313.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILAEGDRVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQFASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            5995..6435
                     /locus_tag="VW144_000843"
     CDS             5995..6435
                     /locus_tag="VW144_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU9600314.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQTSLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQGQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6489..7901
                     /gene="sufB"
                     /locus_tag="VW144_000844"
     CDS             6489..7901
                     /gene="sufB"
                     /locus_tag="VW144_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU9600315.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8132..9373)
                     /locus_tag="VW144_000845"
     CDS             complement(8132..9373)
                     /locus_tag="VW144_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU9600316.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIEQI
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9476..10585
                     /locus_tag="VW144_000846"
     CDS             9476..10585
                     /locus_tag="VW144_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9600317.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGVFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            11231..11578
                     /gene="ssrA"
                     /locus_tag="VW144_000847"
     tmRNA           11231..11578
                     /gene="ssrA"
                     /locus_tag="VW144_000847"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(11748..11835)
                     /locus_tag="VW144_000848"
     tRNA            complement(11748..11835)
                     /locus_tag="VW144_000848"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11799..11801),aa:Ser,seq:gga)
     gene            11997..12737
                     /locus_tag="VW144_000849"
     CDS             11997..12737
                     /locus_tag="VW144_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9600318.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            12734..13645
                     /gene="pfkB"
                     /locus_tag="VW144_000850"
     CDS             12734..13645
                     /gene="pfkB"
                     /locus_tag="VW144_000850"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEU9600319.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSDGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            13642..15594
                     /locus_tag="VW144_000851"
     CDS             13642..15594
                     /locus_tag="VW144_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9600320.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            15758..17983
                     /locus_tag="VW144_000852"
     CDS             15758..17983
                     /locus_tag="VW144_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU9600321.1"
                     /translation="MLISLGIAILLIFAVFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKEIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(18027..18461)
                     /locus_tag="VW144_000853"
     CDS             complement(18027..18461)
                     /locus_tag="VW144_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600322.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNTKKEPTTSQTTTIVKEIQKESPQENTSPNKENKTSQEKTQQEETPKSSVKE
                     EKKEEQKTSTQDSSTPAPTPSKPATENEKQSNTPTSENKTNQ"
     gene            18624..20708
                     /locus_tag="VW144_000854"
     CDS             18624..20708
                     /locus_tag="VW144_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9600323.1"
                     /translation="MFEIRNLSLSFAGRRLLEPTDLIFERGKVYTIYGKSGVGKSSLL
                     NKIGLLSATDPSVSYFFDGKEIDIENKKSVSVFIAEEVAFVFQGRNLINDLTVFENLR
                     LALNFYDLSPDEIESKIDTVLADLQISSLKHAYPEDLSGGEEQRVAIARALVTGKTLI
                     LADEPTSSLDKENREKISALLIDLAHKYHKIVLIVSHDEAMIAIGDVQLHFKDHKLVG
                     NCLILNSDEGIKDSKKWSSCLLSSHTKLSVLKHRRPFLPRLLAFFISLVVAIAVTSIN
                     FQSLFADKYHQLVNNSLENGFLVINDSLHLQTTKVLDDFLSFDKEEITSISTSQNIQT
                     VNPYIEFLSLGMTFDNSKDYSQLQKNFQPTLTIDGQTRSLTRNFSIQPLYQTNTTQRQ
                     IEYFDRSNEKGIYLSEQFISDEKLPNIVSGTTVTLTFYIPISLYESSIEKEGNILKGD
                     GGLFVKVDKTYPVLGIVKKSYPFEYSPYGNTLFMNIAEMEELQNEAIQQHPMTKMALD
                     GFPLKSWMPSAIHVLLKDSGAIPEELSRIRAISSQITILSSYENYEEFNKGLTYIRQF
                     LMLLSLVLLILVIAVLSFVFFLLNRPRRFEVGILKSLGYSTQNIVWLFLKELIGYGKT
                     ISILASCLLVILSILAMQVLKLEISDVFKFYLTSVFTLIGLSVSVLIISGLLPIYTTC
                     RQTVVDTIRKNG"
     gene            20718..21095
                     /locus_tag="VW144_000855"
     CDS             20718..21095
                     /locus_tag="VW144_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600324.1"
                     /translation="MKHKIMKKIIVVVFAIPWLTFLSQVHADYVSKSSSLDGYSLVGL
                     NVLNVTFNASGYSDTYSGSVSNMYFTDWAWFPNTISNRSTWKSSIPYGQRANGTVHIG
                     VGVNSPWGAVNLWGGQNYFSLDF"
     gene            21255..22397
                     /locus_tag="VW144_000856"
     CDS             21255..22397
                     /locus_tag="VW144_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9600325.1"
                     /translation="MIYFDNSATTRPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWIIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKAQGFEVDFAPVDNKGFVDVEALEDLIRPDTTLVSIMAVNNEIGSVQPIEAI
                     SKLLADKPTISFHVDAVQALAKIPTEKYLTERVDFATFSGHKFHGIRGVGFIYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            22406..23620
                     /gene="thiI"
                     /locus_tag="VW144_000857"
     CDS             22406..23620
                     /gene="thiI"
                     /locus_tag="VW144_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEU9600326.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSAVQEIMQDIYKE
                     GMTFKISSKRSDHTFELDSRELNQTLGGAVFEAIPNVQAQMKSPDINLQVEIREEAAY
                     LSYETVRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            23755..24568
                     /locus_tag="VW144_000858"
                     /pseudo
     CDS             23755..24568
                     /locus_tag="VW144_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            24590..24925
                     /locus_tag="VW144_000859"
     CDS             24590..24925
                     /locus_tag="VW144_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEU9600327.1"
                     /translation="MHIEHLSHWSGHPNREMYLNRYGHGGIPVVVFASSGGSHNEYYD
                     FGMIDACASFIEEGRVQFFTLSSVDSESWLATWKNAHDQAEMHRAYERYVIEEAILLS
                     STRQVSLMA"
     gene            24922..25329
                     /locus_tag="VW144_000860"
     CDS             24922..25329
                     /locus_tag="VW144_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU9600328.1"
                     /translation="MMTTGCSMGAYHALNFFLQHPDVFTKVIALSGVYDARFFVGDYY
                     NDDAIYQNSPVDYIWNQNDGWFIDRYRQAEIVLCTGLGAWEQDGLPSFYKLKEAFDQK
                     QIPAWFAEWGHDVAHDWEWWRKQMPYFLGNLYL"
     gene            25342..26508
                     /locus_tag="VW144_000861"
     CDS             25342..26508
                     /locus_tag="VW144_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9600329.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
     gene            complement(26999..28669)
                     /locus_tag="VW144_000862"
     CDS             complement(26999..28669)
                     /locus_tag="VW144_000862"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU9600330.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVDAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAAL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWGKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            28899..29588
                     /gene="coaB"
                     /locus_tag="VW144_000863"
     CDS             28899..29588
                     /gene="coaB"
                     /locus_tag="VW144_000863"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEU9600331.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISAGQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            29600..30151
                     /gene="coaC"
                     /locus_tag="VW144_000864"
     CDS             29600..30151
                     /gene="coaC"
                     /locus_tag="VW144_000864"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEU9600332.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            30135..30698
                     /locus_tag="VW144_000865"
     CDS             30135..30698
                     /locus_tag="VW144_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9600333.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGITLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            30980..31507
                     /locus_tag="VW144_000866"
     CDS             30980..31507
                     /locus_tag="VW144_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9600334.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEILACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            31519..32034
                     /locus_tag="VW144_000867"
     CDS             31519..32034
                     /locus_tag="VW144_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEU9600335.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFKPLSELTDGVHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(32031..32498)
                     /locus_tag="VW144_000868"
     CDS             complement(32031..32498)
                     /locus_tag="VW144_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9600336.1"
                     /translation="MSRAQLTILTNICLIEDLEAQRVVMQYRAPETNRWSGYAFPGGH
                     VENGESFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVVCYKATEFSGTLRS
                     SDEGEVSWVQKDQIPNLDLAYDMLPLMEMMEASDKSEFFYPRRTEDDWEKKIF"
     gene            complement(32571..33059)
                     /locus_tag="VW144_000869"
     CDS             complement(32571..33059)
                     /locus_tag="VW144_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9600337.1"
                     /translation="MTVIIKSMETPEEIEGKPLVHWQTWREAYDGLLPAEFQETMTLE
                     RCRFFSQKYPENTLIAMDGMKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LIKAALIALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDEQEKILELGKPIKEKRMVFY
                     SK"
     gene            complement(33025..33588)
                     /locus_tag="VW144_000870"
     CDS             complement(33025..33588)
                     /locus_tag="VW144_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9600338.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKTNDGYYQINGNS"
     gene            complement(33598..35586)
                     /gene="uvrB"
                     /locus_tag="VW144_000871"
     CDS             complement(33598..35586)
                     /gene="uvrB"
                     /locus_tag="VW144_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEU9600339.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            complement(35663..36616)
                     /locus_tag="VW144_000872"
     CDS             complement(35663..36616)
                     /locus_tag="VW144_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9600340.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLATYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENCLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
BASE COUNT        10864 a         7139 c         7617 g        11013 t
ORIGIN      
        1 tttctaatga gttgtttgat cgcttttcat tataggtcat atgggacttt ttttctacac
       61 aaaaataggc tccataatat ccatagggga tttacccact acaaatatta tagagcccaa
      121 aatcctttgt ttactaaaca agggattttt cttttgtctc tgctcctttt ttgatataat
      181 agttctatgt taaaatcaga aaaacaatca cgttatcaaa tgttaaatga agaattgtcc
      241 ttcctattgg aaggcgaaac caatgttttg gctaatcttt ccaacgccag tgctctcata
      301 aaatcacgtt ttcctaatac cgtatttgca ggcttttatt tgttcgatgg aaaggaattg
      361 gttttaggcc ccttccaagg aggtgtttcc tgcatccgta ttgcactagg caagggtgtt
      421 tgtggtgagg cagctcactt tcaggaaact gttattgttg gagatgtgac gacctatctc
      481 aactatattt cttgtgatag tctagctaaa agtgaaattg tggtgccgat gatgaagaat
      541 ggtcagttac ttggagttct ggatctggat tcttcagaga ttgaggatta cgatgctatg
      601 gatcgagatt atttggaaca atttgtcgct attttgcttg aaaagacagc atgggacttt
      661 acgatgtttg aggaaaaatc ttaatgtatc aagcacttta tcgaaaatat agaagtcaaa
      721 acttctccca gttagttggt caagaagttg tggctaagac tcttaaacaa gcggtggagc
      781 aagagaaaat aagtcacgct tatctttttt ctggtcctcg tggaacggga aaaaccagtg
      841 ttgctaaaat ctttgccaag gctatgaact gtcccaatca agtgggtggc gaaccttgca
      901 ataactgcta tatttgtcaa gcagtgacgg acggtagttt agaagatgtc attgaaatgg
      961 atgcagcttc taataatggg gtagatgaaa ttcgcgaaat tcgtgataaa tctacctatg
     1021 cgcctagcct tgctcgttat aaggtttata tcatagatga ggttcacatg ctgtctacag
     1081 gggcttttaa tgccctccta aagacgctgg aagaaccaac acagaatgta gtctttattt
     1141 tggccactac tgaattgcac aagattcctg ctactattct atcccgtgtg caacgttttg
     1201 agtttaaatc aattaagaca caggatatta aggaacatat tcactatatc ttagaaaaag
     1261 aaaatatcag ttctgaacca gaggctgtgg aaatcattgc cagacgggcg gaaggtggaa
     1321 tgcgggacgc cttgtctatt ttggatcaag ccctgagttt gacacaggga aatgagctga
     1381 cgactgctat ctctgaagaa attactggca ccattagcct atcagccttg gatgattatg
     1441 tggcggcctt gtctcaacag gatgttccca aagctttgtc ttgcttgaat cttctttttg
     1501 acaatggtaa gagcatgact cgttttgtga ccgatctttt gcactattta agagacttgt
     1561 taattgttca aacaggggga gaaaatactc atcatagttc agtctttgta gaaaatttgg
     1621 cacttcctca aaaaaatctg tttgaaatga ttcgcttagc aacagtgaat ttagcagata
     1681 ttaagtctag tttgcagccc aagatttatg ctgaaatgat gaccgtccgt ttggcggaaa
     1741 tcaagcccga accagctcta tcaggagcgg ttgaaaatga aattgctacg ctgagacagg
     1801 aagttgcccg tctcaaacaa gagctttcta atgcaggtgc ggttcctaaa caagttgcac
     1861 cagctcctag tcgaccagct acgggcaaaa cagtctatcg tgtcgatcgc aataaagtgc
     1921 aatctatctt acaagaggcc gtcgaaaatc ctgatttagc acgtcaaaat ctaattcgtt
     1981 tgcagaatgc ctggggagag gtaattgaaa gtctaggtgg gccggacaag gctctgctag
     2041 ttggttctca accggttgct gccaatgaac accatgctat tcttgctttt gagtctaact
     2101 tcaatgctgg tcaaactatg aaacgagaca atctcaatac catgtttggt aatatcctca
     2161 gtcaggcggc aggtttttca cctgagattt tagctatttc catggaggaa tggaaagaag
     2221 ttcgcgcagc cttttcagcc aaagccaaat cttctcaaac tgaaaaagaa gtagaagaaa
     2281 gcctgattcc agaaggattt gaatttttgg ctgataaagt gaaggtagag gaagactaaa
     2341 gaaagatttc atgatacaat aagtttatga ataaacaaca atttattatt atggcgctat
     2401 ttacagctgc tgagacctat tttttcaatg aagcctggat gactggccgc tatattatgg
     2461 cagccttttg ggcaatttta ctctttagaa atttccgagt cagttatgtg atgggtaaaa
     2521 ttgttgatgt catcgatcag cattttaata ggaaagacta gccctcagct tccagacaaa
     2581 atcaaagcct tttaggcttt tttttgttat actagaaaag tatatttata gaatttttgc
     2641 tctatttctg gggaaatcag acgtttttct agtaagtact gtaaaagttt tgaaaaagaa
     2701 aggaactatc atgtcagtaa tagagatcaa agatcttcac gttgagattg aaggaaaaga
     2761 aattttaaaa ggggttaacc tgaccctgaa aacaggagaa attgccgcta tcatgggacc
     2821 aaatggtaca ggtaaatcga ctctttctgc cgctatcatg ggaaatccaa actatgaagt
     2881 aactaaaggt gaagttttgt ttgatggcgt aaacatcctt gagttggaag tggatgagcg
     2941 tgcgcgtatg ggacttttcc ttgctatgca atacccatca gaaatccctg gaattaccaa
     3001 tgctgagttt cttcgtgccg ctatgaatgc gggtaaagaa gatgatgaga agatttcagt
     3061 tcgtgagttt attactaagc tagatgaaaa aatggaattg ctcaacatga aagaagaaat
     3121 ggcagagcgt tacctcaacg aaggcttctc tggtggtgag aaaaaacgca atgaaattct
     3181 tcaacttttg atgttggagc caacatttgc tcttttggac gagattgact caggtcttga
     3241 tattgacgct cttaaagttg tgtctaaagg tgtcaatgcc atgcgtggtg aaggttttgg
     3301 tgctatgatc atcactcact accaacgtct tttgaactat atcacacctg atgtggtaca
     3361 cgtgatgatg gaaggtcgtg ttgtcctttc tggtggtcca gaattggctg cgcgtttgga
     3421 acgtgaagga tacgcaaaac tagctgaaga acttggctac gactacaagg aagaattgta
     3481 attccctcgt atcttttagg agaagtaaat gactagagaa aatattaaac ttttttcaga
     3541 aatgcacgct gaaccaagct ggttggctga tctccgtcaa aaagcttttg acaagattga
     3601 gactttggaa ttaccagtta ttgagcgtgt caaattccac cgttggaatc tgggtgatgg
     3661 aacgattaca gaaaatgagc catcagcaaa tgttccagat ttcacagctt tagatcatca
     3721 cttgaagttg gtgcaagtag gaactcaaac tgttttcgaa caaactccag ttgagttagc
     3781 tgaacagggt gttgtcttca cagactttca ctcagcttta gaagaaattc cagagctgat
     3841 cgaagaattc ttcatgtcat ctgttaagta tgatgatgac aagttggcgg cttaccacac
     3901 agcttacttt aacagtggtg ctgtactcta tattccagat aacgtagaaa tcacagagcc
     3961 aattgaagga attttctacc aagatagcga tagcaatgtg ccgtttaaca agcatattat
     4021 gattatcgct ggtaaaaatt ctaagattag ttatctggag cgtttagagt cacgcggtga
     4081 aggaagtgac aaagtaactg ccaatatcac agtggaagtg attgcacgtt ctggtgcgca
     4141 agtcaagttt gctgctatcg accgtctagg tgaaaacgtc actgcctaca ttagccgtcg
     4201 tggtaaatta ggcaacgatg caagtattga ctgggctatc ggtgtcatga acgaaggaaa
     4261 tgtcgttgct gattttgata gtgacttgat tggtaatggt agccatgctg acctcaaggt
     4321 tgtagctctt tcaagtggtc gtcaggtaca agggattgat actcgtgtaa ctaactatgg
     4381 ctgcaactct atcggaaata tcctacaaca tggggttatc cttgaaaaag caactttgac
     4441 tttcaatggt atcggccaca tcatcaagga tgctaaggga gcagatgcgc aacaagagag
     4501 ccgtgttctc atgctttcag accaagcgcg ttcagatgct aacccaattc ttttgattga
     4561 tgaaaatgat gtcactgcag gacacgcagc gtctatcgga caagttgatc cagaagacat
     4621 gtattacctc atgagtcgtg gattggataa ggcaactgca gagcgtttgg ttgttcgtgg
     4681 tttccttgga tctgttatcg tggagattcc agtcaaggaa gttcgtgatg aaatgattgc
     4741 aactatcgaa gagaaattgt caaaacgcta aggggaagcc tatgttagat gtagaagcga
     4801 ttcgcaagga ttttccaatt ttagatcaga ttgtcaatga tgaacctctg gtctatctgg
     4861 acaatgctgc gacgacacaa aaaccactag tagttctgaa agctattaac agttactatg
     4921 agcaggacaa tgccaatgtt caccgtggtg tccatacctt agcggaacga gcgacagctt
     4981 cttatgaagc tgctcgtgaa accattcgta agtttattaa tgcaggctct acaaaggaag
     5041 ttctctttac cagaggaacg acaaccagcc ttaactgggt ggcacgcttt gctgaggaaa
     5101 ttctcgctga gggagaccgg gtcttgattt cagtaatgga acaccattct aatatcattc
     5161 catggcagga agcttgtcga aagactggag cagagcttgt ctatgtctat cttaaagacg
     5221 gtgccttgga tatggaggat ttgcgagcta aattgactga taaggttaaa tttgtttccc
     5281 tagctcatgc ctccaatgtt cttggtgtgg tcaatccgat caaggaaatc actcaattag
     5341 cccaccaagt tggggcaatt atggtagtgg atggtgctca atctacacct catatgaaga
     5401 ttgatgtcca ggacttggat ctggactttt tcgccttttc gggtcacaag atggctggtc
     5461 cgactggtat cggtgtcctt tacggcaaag aaaagtatct tgagcaaatg tctccagtag
     5521 aatttggcgg cgagatgatt gattttgtct acgagcaatt tgctagttgg aaggaattgc
     5581 cttggaaatt tgaggctgga acgccaaata tggcaggagc tattggactt gcgactgcag
     5641 ttgattatct ggaaaagatt ggtatggatg ccgttgaagc tcatgaacag gaattgattg
     5701 cgtacgtcta tccaaaactg caggcaattg agggattgac catttacggt tctcaggatt
     5761 tggctcaacg ttcgggtgtt attgccttta acctaggtga tctccatcct cacgatcttg
     5821 cgacggctct ggattatgaa ggagtggctg ttcgtgctgg tcaccattgt gcgcaaccct
     5881 tgcttcagta tttggaagtc ccagcaacag ctcgtgcaag tttttatatc tacaatacca
     5941 aggcagattg cgacaaacta gtcgatgccc tacaaaagac aaaggagttt ttcaatggca
     6001 ctttctaaac tagatagcct ttatatggca gtggtagcag accattcgaa aaatccacat
     6061 caccaaggga agttagaaga tgctgagcaa accagtctca acaatccgac ttgtggggat
     6121 gtcatcaacc tctctgtcaa gtttgatgca gaggaccgtt tggaagatat tgcttttcta
     6181 aattcaggat gcacgatttc aactgcttct gctagtatga tgacagatgc cgttttagga
     6241 aaaaccaaac aagaaatttt agaactggcg actatttttt ctgaaatggt tcaagggcaa
     6301 aaagatgagc gtcaaggcca acttggagac gcggcattct tgtcaggtgt tgccaaattc
     6361 cctcaaagaa tcaagtgtgc aaccctagct tggaatgccc ttaagaaaac aattgaaaat
     6421 caagaaaaac agtaagacaa gtttcttttg tcttatgaat tattagaaat gaagaaagaa
     6481 aggatactat ggctgaagaa agagtagaac caaaaccaat tgaccttggt gaatataaat
     6541 ttggtttcca tgacgatgta gagcctgtct tatcgacagg aaaaggactc aacgaaggtg
     6601 ttattcgtga attatctgct gctaagggtg agcctgagtg gatgttggag ttccgtttga
     6661 agtcttatga aaccttcaaa aaaatgccca tgcaaacttg gggagcagac ttgtcagaga
     6721 ttgactttga tgacttaatc tactaccaaa aaccatctga caaaccagcc cgttcttggg
     6781 atgatgtacc tgaaaagatt aaagaaacct ttgaacgtat cgggattcca gaagctgaac
     6841 gtgcttattt agcaggggct tctgcccagt acgagtcaga agtggtttac cacaacatga
     6901 aggaagagtt ccaaaaatta ggtattatct ttacagatac agattccgca ctcaaggaat
     6961 acccagactt atttaaacaa tactttgcga agttggtacc gccgacagat aacaagttgg
     7021 cagccctcaa ctcagcagta tggtcgggtg gaacttttat ctacgtgcca aaaggtgtca
     7081 aggtagatat tccacttcaa acttatttcc gtatcaataa cgaaaatata ggtcagttcg
     7141 aacgtacctt gattatcgtt gatgagggag caagcgtcca ctacgtagaa ggatgtacag
     7201 caccaacata ttcaagcaat agcttacacg ctgccattgt agaaattttt gctttggacg
     7261 gagcttatat gcgttataca actatccaaa actggtctga taacgtctat aacttggtaa
     7321 caaagcgtgc taaggctcaa aaggatgcca ctgttgagtg gattgatgga aacttgggtg
     7381 ccaaaacgac tatgaaatat ccatctgttt accttgatgg agaaggagcg cgtggtacca
     7441 tgctctctat cgcctttgct aatgcagggc aacaccaaga cacgggtgct aagatgattc
     7501 acaatgctcc acataccagc tcgtctattg tgtctaaatc catcgctaaa ggtggaggaa
     7561 aggttgacta ccgtggacaa gtcaccttta acaagaactc taagaaatct gtttcccaca
     7621 ttgaatgtga taccattatc atggatgact tgtcagcatc agatactatt ccatttaatg
     7681 aaattcacaa ctcgcaagtg gctttggaac acgaagccaa agtatctaag atttcagaag
     7741 agcaattgta ttatctcatg agccgtggat tgtcagaatc tgaggcaact gaaatgattg
     7801 tcatgggatt tgtagaaccc tttacaaaag aacttccaat ggaatacgca gttgagctga
     7861 accgcttgat tagctatgaa atggagggat cagttggata aaatttgatt ttatactctt
     7921 cgaaaatctc ttcaagccac gtcagcatcg ccttaccgta tgtatggtta ctgacttcgt
     7981 cagtttcatc tacaacctca aaacagtgtt ttgagcaacc tgcggctagc ttcctagttt
     8041 gttctttgat tttgagtatt agatttactc aaaatcaagg attttgaaga tgaacttgta
     8101 tcaaaaaatc gcggtttaaa atcgcgattt tttataattt ctcgttaaca aagcggacaa
     8161 actgattcca ccaaactttt aagaagaagg ctttttcaat tttcttgtct gctaccattt
     8221 cgaaactagg gcgctctgtg gtgatgtaac cttgaccaat caagtccttg tcttcataag
     8281 tcaaatggcc aaccactgtt ccagcttcaa gtggtgctgg gattgctttg gaatcaggtg
     8341 tgaattgaac agattgggaa gattgattcc caatctgttc gattagatag atatcctctg
     8401 gagccactgc agttactgta tcttcttttc catcttgtac aggggctttg ctatcttgat
     8461 aggcatcgcc ttgttgaacg attttgcgaa gtgtaaatgt agaagaaata taatccatta
     8521 gggaagatgt agctgtaaat cgagcgtaag gattattgtc ttgatgatct gcatttaaaa
     8581 caactgtgat gactctcatg cctttttcga cagtagtacc aacaaaagac tctccagcct
     8641 tatctgttgt tcctgttttt agcccatcaa aaccaccacg gtaagcaggc ataccttcta
     8701 acatgtagtt ggttgaagtg attgtcatcc cagcaaaagt agaagaaggt tttttggtga
     8761 tttctaagac ttgtgggtat tttttgatga ggttgcgagc aacgatagcg acatcataag
     8821 cactaagctt attttcctca tcttttttag aacctgggta aatgttatcc cctagagttt
     8881 cattgttaag acctgtcgta ttgacaacag tggcatcctg aattccccat tccaagagtt
     8941 ttgcccgcat catatcgacg aaatcttttt ctgagccagc aattttctca gctagggcaa
     9001 tagcggcgct gttggcacta gataccagag ttgcttcaag caactcttcg acagtataat
     9061 tacgggcctc cataggaata ttactggctt cagaatttgt cgtcaattga taaggataat
     9121 cagaaatatc tacaggagtg gagagggtaa tacttccgtt ttccaaagct tcatagacca
     9181 gataaacagt aatcaatttt gttatggaag caatttcgac aggttgcgtt gcatccttct
     9241 catagagaat tttaccagta tttgcctcaa cagcaatcgc atgtttagcg gcaatggtaa
     9301 aatcttgagc aacagcagta gaagcacccc ctaaaagaga gacagttaac aaagttaaaa
     9361 atattttttt catagtagtc ttattctatc ataaagaaaa aaaatattct tgctttaata
     9421 attcatctgt taagcttttt gaaaatatgg taaaataaag tgagggaggt aactcatgtt
     9481 tcgtagaaat aaattatttt tttggaccac agaaatttta ctcttaacca tcatctttta
     9541 cctatggaga cagatggggt ctttgattaa cccttttgtt agcgtgctta atacaattat
     9601 gattccattt ttattagggg tctttcttta ttatttgaca aaccctattg ttactttctt
     9661 aaataaagtc tgtaaactca atcgtttgct tggtatttta attaccttgt gtactttggt
     9721 ctggggaatg gtcataggtg ttgtctatct cttacctatt ttgattaatc agttatctag
     9781 tttgattata tctagtcaaa ctatttatag tcgagtacaa gacttaatca tagacttatc
     9841 taattatcct gcgctccaga atttggatgt agaagctaca attcagcagt taaacttatc
     9901 ctatgttgat attcttcaaa atatcctaaa tagcgtatca aatagtgtgg ggagcgtctt
     9961 gtcagctctt atcagtactg ttttgatttt gattatgact ccagtttttt tggtttattt
    10021 cttattagat ggacataaat tcttgcccat gcttgaaaga acgattctaa agagggatcg
    10081 cttgcatatt gcaggcttat taaagaattt aaatgcgacg attgctcgct atattagtgg
    10141 agtttcgatt gacgcaatca ttataggttg tttagcttat attggctata gtattattgg
    10201 tttaaaatat gctttagttt ttgccatttt ttctggtgta gccaatttaa ttccttatgt
    10261 ggggccaagt attggtttga ttcctatgat catcgcaaat atattcactg atccccatag
    10321 actgctgatt gcagtgattt atatgcttgt tgttcagcag gtagatggca atatcttata
    10381 tcctcgaatc gtaggaagtg ttatgaaggt tcatccaatc acgattttag ttttactttt
    10441 gttgtcaagc aatatttatg gtgtagttgg aatgattgtc gcagtgccaa cctattctat
    10501 cttgaaagaa atttctaagt tcttatcccg tttgtatgaa aatcataaaa taatgaaaga
    10561 acgagaaaga gaattagcta agtaaaagtc aggagaaccc tgatttttct ttactggaag
    10621 tggcctttag attagaagac tgaaaataag ttaaagtctt aaactaattt tcacagctaa
    10681 gaatagtaga agttaatctg ataaaaatcg aaaaaaccag tggaattctg tgtcagggta
    10741 agttccactg gttttcatag tctattaaag ttcgaatgaa acctatttat agtagattga
    10801 aactagaata gtacacctct aattctaaaa cattgttaga aatcgatttg actgtcctga
    10861 tctattcgtc ctattcttat ttcattttac tatattttgg tgcaataagt gaaaagtagt
    10921 ccgaataata taaggattga ttttatagtt tttaaactca aatgaattaa aataaagaga
    10981 gtacgaaaat tctcatctga aagtatttta gaataattct cttcgtgaat ttcttcaaaa
    11041 cagatagctt catcttaggt atgtgttaat tggatttcgt aagctctatt tacaatcgta
    11101 aagttatgca ctgagaaagt tgtagttagt cttccagttt gctaaatttc tcgaatagta
    11161 tcttttttag gtatgtgatt tctttttgca tttttgagtt agataaggta taatggtttt
    11221 attgtctttt ggggtcgtta cggattcgac aggcattatg aggcatattt tgcgactcgt
    11281 gtggcgacgt aaacgctcag ttaaatataa ctgcaaaaaa taacacttct tacgctctag
    11341 ctgcctaaaa accagcaggc gtgacccgat ttggattgct cgtgttcaat gacaggtctt
    11401 attattagcg agatacgatt aagccttgtc tagcggcttg ataagagatt gatagactcg
    11461 cagtttctag acttgagtta tgtgtcgagg ggctgttaaa ataatacata acctatggtt
    11521 gtagacaaat atgttggcag gtgtttggac gtgggttcga ctcccaccgg ctccattatt
    11581 cctttgcatt cttttgcatt ccttggtaaa acgttgttaa atcaacgttt tttattttta
    11641 tctttggtat tcctttgcat tcttttgcta aaaagggagt cacaaacaga ccctatttta
    11701 aaaaaggata caacatttgt tgcatcctaa aaataatctt ttttcgacgg aagatatggg
    11761 attcgaaccc acgcacgcta ttacacgcct accgcgtttc caacacggcc tcttaagcct
    11821 cttgagtaat cttccaatac ttactcaaat agtctaccat aaaggctctt atcttgcaat
    11881 aaaaattcta gaaataagaa aaatgataga ttttgaaaga aaatgataaa aaatgcttga
    11941 ctttgaaata aagtatggta gaatgaatag tgtaaacgat aacaggaggt gattcagtgt
    12001 taaaaacaga acgtaaacaa ctgattctag aggagttaaa tcaacatcat gtagtttctc
    12061 tagaaaaatt agttagtttg ctagaaacgt cagaatcaac ggttcgaaga gacttggatg
    12121 agttggaagc ggaaaacaag cttcgtcgtg tgcatggtgg agcagaactc ccccactcct
    12181 tacaggaaga agaaaccatt caagaaaaat ctgtcaaaaa ccttcaagaa aagaagttgc
    12241 tggctcagaa agcagcctct ctcattaaag aaaaagatgt catctttatc gatgctggaa
    12301 caacaactgc ttttttgatt catgaattgg tcaataagaa tgttacagtt gtgaccaact
    12361 ccattcacca tgccgctcag ttggttgaaa agcagattcc aactgtcatg gttggaggaa
    12421 acgtcaagac ggcgacagat gctagtatcg ggggcgttgc tcttaaccag attaaccaat
    12481 tgcactttga ccgtgccttt atcggaatga atggtgttga cgatggctat tatacgactc
    12541 ctgatatgga ggagggagct gtgaaaagag ctattttgga gaatgccaag cagacctacg
    12601 tcttggtgga ttcgtcaaaa attggacaaa cttgctttgc caaggtagca ccactcaaac
    12661 gcgctatcgt tatcactagt caagggcatg agctcttgca ggttattaag gagaaaacgg
    12721 aggtaataga agtatgattt atacagtcac actcaatcca tccattgact atatcgttcg
    12781 tttggaccaa gtcaaagttg gtagtgtaaa tcgtatggac agtgatgata agtttgctgg
    12841 tgggaaagga atcaatgtca gccgtgtctt gaaacgtttg aatataccaa atacagcgac
    12901 gggatttatc ggtggcttta ctggtaaatt tatcacagat actttagcag aggaagaaat
    12961 cgagacacgt tttgtccagg tggcagaaga tactcgtatc aatgttaaaa tcaaagcaga
    13021 ccaagaaaca gaaatcaacg gaacgggtcc aactgttgaa tcggttcagc tagaagaatt
    13081 gaaagctatt ttatctagtc tgacagcaga agatacagtt gtctttgcag gttcaagtgc
    13141 taaaaatcta ggcaatgtta tctataagga tttgatttcc ttgacgcgcc agactggtgc
    13201 gcaagtggtc tgtgactttg aaggacagac cttaattgat agtttggact accagcctct
    13261 tcttgtaaaa ccaaacaatc atgaacttgg agcgattttt ggggttaaac tcgaaagttt
    13321 agatgaaatt gagaaatacg ctcgtgagtt actggctaag ggtgctcaaa atgttattat
    13381 ctctatggct ggtgatggtg cccttcttgt cacatctgat ggagcttact tcgctaaacc
    13441 aatcaaggga acagtcaaaa attcagttgg agctggtgat tctatggttg ctggattcac
    13501 aggtgaattt gtcaaatcaa aagacgcagt agaagccttc aaatggggag tggcttgcgg
    13561 aacggcaact accttctcag atgacttggc aacggcggaa tttattaaag aaacatatgg
    13621 aaaagttgag gtagaaaaac gatgaaaatt caagacctat tgagaaaaga tgtcatgttg
    13681 ctagatttgc aggcaactga aaaaacagct gtcatcgacg agatgattaa aaatttgaca
    13741 gaccacggtt atgtaacaga ttttgaaaca tttaaagaag gcattttggc gcgtgaagct
    13801 ttgacttcta ctggtttggg tgatggaatc gcaatgcctc acagcaaaaa cgctgctgtc
    13861 aaagaagcga cagttctatt tgctaagtca aataagggtg ttgactacga gagcttggat
    13921 ggacaagcaa ctgacctctt cttcatgatt gcagctccag aaggtgccaa tgatactcac
    13981 ttggcagcct tggcagaatt gtctcaatac ttgatgaaag acggttttgc agacaaactt
    14041 cgtcaagcaa catctgcaga ccaagttatc gaactttttg accaagcttc agaaaaaact
    14101 gaggaacttg ttcaagcacc tgctaatgac tctggtgact ttatcgtagc tgttacagct
    14161 tgtacaacag gtattgccca cacttacatg gcccaagaag cccttcaaaa agtagctgct
    14221 gaaatggggg ttggtatcaa ggtcgaaacc aacggtgcta gcggtgttgg aaatcaacta
    14281 actgcagaag atatccgtaa ggctaaagct attatcattg cagcagacaa ggccgttgaa
    14341 atggatcgat ttgatggaaa accattgatc aatcgtccag ttgctgacgg tatccgtaag
    14401 acagaagagc taattaactt ggctctttca ggagatactg aagtctaccg tgccgctaat
    14461 ggtgccaaag ctgcaacagc ctctaacgaa aaacaaagcc ttggtggtgc cttgtacaaa
    14521 cacttgatga gtggtgtatc tcaaatgtta ccattcgtta tcggtggtgg tatcatgatt
    14581 gcccttgcct tcttgattga cggtgctttg ggtgttccaa atgaaaacct tggcaatctt
    14641 ggttcttacc atgagttagc ttctatgttc atgaaaattg gtggagctgc ctttggtttg
    14701 atgcttccag tctttgcggg ttatgttgcc tactctattg ctgaaaaacc gggtttggta
    14761 gcaggtttcg tggctggtgc tattgccaaa gaaggttttg cctttggtaa aattccttat
    14821 gccgcaggtg gtgaagcaac ttcaactctt gcaggtgtct catctggttt cctaggtgcc
    14881 cttgttggtg gatttatcgc aggtgccttg gttcttgcca tcaagaaata cgttaaagtt
    14941 cctcgttcac tcgaaggtgc taaatcaatc cttctattgc cacttcttgg aacaatcttg
    15001 acaggatttg ttatgctagc tgtgaatatc ccaatggctg caatcaacac tgctatgaat
    15061 gacttcctag gcggtcttgg aggaggttca gctgtccttc ttggtatcgt ccttggtgga
    15121 atgatggctg ttgacatggg tggaccagtt aataaagcag cttatgtctt tggtacaggt
    15181 acgcttgcag caactgtttc ttcaggtggt tctgtagcca tggcagcagt tatggctgga
    15241 ggaatggtgc caccacttgc aatctttgtc gcaactcttc ttttcaaaga taaatttact
    15301 aaggaagaac gtaactctgg tttgacaaac atcatcatgg gcttgtcatt tatcactgag
    15361 ggagcgattc catttggtgc cgctgaccca gctcgtgcga ttccaagctt catccttggt
    15421 tcagcagtag caggtggact cgttggtctt actggtatca aactcatggc gccacacgga
    15481 ggaatcttcg ttatcgccct tacttcaaat gctctccttt acctcgtttc tgtcttggta
    15541 ggagcaatcg taagtggtgt ggtttatggt tacctacgca aaccacaagc ataaaaaata
    15601 gaaaaatgaa aagattggac cgtttggtgc agtctttttc tcttcccgaa atgcctgtga
    15661 aatatggtat aatagaagaa tggcaaacaa gaatacaagt acaacaagac ggagaccgtc
    15721 taaagcagaa ctggaaagaa aagaagcgat tcaacgaatg ttgatttcat taggaattgc
    15781 gattttattg attttcgcag tcttcaaatt aggggctgca ggtataaccc tttataattt
    15841 aattcgcttg ctagtgggta gcctagctta tctggcgata ttcggcctat taatctatct
    15901 cttctttttc aagtggatac gaaaacagga aggactctta tctggctttt tcaccatatt
    15961 tgctggctta ctcttgattt ttgaagccta cttggtttgg aaatatggtt tggacaagtc
    16021 cgttctaaaa gggaccatgg ctcaggttgt gacagatctg actggttttc gaacgactag
    16081 ctttgctgga gggggcttga taggggtcgc tctttatatt ccaacagcct ttctcttttc
    16141 aaatatcgga acttacttta ttggttctat cttgatttta gtgggttctc tcctagttag
    16201 cccttggtct gtttacgata ttgctgaatt tttcagtaga ggctttgcca aatggtggga
    16261 agggcacgag cgtcgaaaag aggaacgctt tgtcaaacaa gaagaaaaag ctcgccaaaa
    16321 ggctgagaaa gaggctagat tagaacaaga agagactgaa aaagccttac tcgatttgcc
    16381 tcctgttgat atggaaacgg gtgaaattct gacagaggaa gctgttcaaa atcttccacc
    16441 tattccagaa gaaaagtggg tggaaccaga aatcatcctg cctcaagctg aacttaaatt
    16501 ccctgaacag gaagatgact cagatgacga agatgttcag gtcgattttt cagccaaaga
    16561 agcccttgaa tacaaacttc caagcttaca actctttgca ccagataaac caaaagatca
    16621 gtctaaagag aaggaaattg tcagagaaaa tatcaaaatc ttagaagcaa cctttgctag
    16681 ctttggtatt aaggtaacag ttgaacgggc cgaaattggg ccatcagtga ccaagtatga
    16741 agtcaagccg gctgttggtg taagggtcaa ccgcatttcc aatctatcag atgacctcgc
    16801 tctagccttg gctgccaagg atgtccgaat tgaagcacca atccctggga aatctctaat
    16861 cggaattgaa gtgcccaact ccgatattgc cactgtatct ttccgagaac tatgggaaca
    16921 atcgcaaacg aaagcagaaa atttcttgga aattccttta gggaaggctg ttaatggaac
    16981 cgcaagagct tttgaccttt ctaaaatgcc ccacttgcta gttgcaggtt caacgggttc
    17041 agggaagtca gtagcagtta acggcattat tgctagcatt ctcatgaagg cgagaccaga
    17101 tcaagttaaa tttatgatgg tcgatcccaa gatggttgag ttatctgttt acaatgatat
    17161 tccccacctc ttgattccag tcgtgaccaa tccacgcaaa gccagcaagg ctctgcaaaa
    17221 ggttgtggat gaaatggaaa accgttatga actctttgcc aaggtgggag ttcggaatat
    17281 tgcaggtttt aatgccaagg tagaagagtt caattcccag tctgagtaca agcaaattcc
    17341 gctaccattc attgtcgtga ttgtggatga gttggctgac ctcatgatgg tggccagcaa
    17401 ggaagtggaa gatgctatca tccgtcttgg gcagaaggcg cgtgctgcag gtatccacat
    17461 gattcttgca actcagcgcc catctgttga tgtcatctct ggtttgatta aggccaatgt
    17521 tccatctcgt gtagcatttg cggtttcatc aggaacagac tcccgtacga ttttggatga
    17581 aaatggagca gaaaaacttc ttggtcgagg agacatgctc tttaaaccga ttgatgaaaa
    17641 tcatccagtt cgtctccaag gctcctttat ctcggatgac gatgttgagc gcattgtgaa
    17701 cttcatcaag actcaggcag atgcagacta cgatgagagt tttgatccag gtgaggtttc
    17761 tgaaaatgaa ggagaatttt cggatggaga tgctggtggt gatccgcttt ttgaagaagc
    17821 taagtctttg gttatcgaaa cacagaaagc cagtgcatcc atgattcagc gtcgtttgtc
    17881 agttggattt aaccgtgcga cccgtctcat ggaagagctt gagatggcag gtgtcattgg
    17941 tccagctgaa ggtaccaagc caagaaaagt attgcagcaa taaaaaatag cttctttcca
    18001 agtttggaag gaggctattt tagtgattat tgattagttt tattttccga agttggggta
    18061 ttggactgtt tttcgttttc agtagcaggt ttacttggag taggagcagg agtagaagaa
    18121 tcctgagttg atgttttctg ctcttctttt ttctcttcct tgacgctgga ttttggcgtt
    18181 tcttcttgtt gtgttttttc ttgagaagtc ttattttcct tattgggact agtattttcc
    18241 tgaggggatt ccttttgaat ttctttgaca attgttgtcg tctggcttgt tgtaggttcc
    18301 tttttagtat ttttgttatt atccaaggcg ttcataatcg tgatactagc ggtaatcaag
    18361 ccaaggatac tagcgatagt cgcaacggtt ttttgaaaga ctgtaaagcc ttttttgatg
    18421 tgttctgttt ttggttttga agttctacga taattagaca tacattctct cctttaatta
    18481 aaatttatta tacctcattt ctttaaaaaa atcttaaaaa aagaggagat ttttctacac
    18541 gaacttcagt aattagttga aataaaatat gacttatgat ataacaaaat aaagaaagcg
    18601 atttcaaaaa aggagggcgt tatatgttcg aaattagaaa tttgagtctc tcttttgcag
    18661 gcaggcggtt gctagagcca acagatttga tatttgaaag gggaaaagta tatacaatat
    18721 atgggaagtc aggcgtagga aagtcttctt tactgaataa aattggtttg ctctctgcta
    18781 cggatcctag tgtttcttat ttcttcgatg gaaaagagat tgatatcgaa aataagaaat
    18841 cagtctctgt ctttatagct gaagaagttg cctttgtatt tcaggggcga aatctaataa
    18901 atgatttgac cgtatttgaa aatttaagac tagctttaaa cttttatgac ttgagtccag
    18961 atgaaataga aagcaaaatt gatacagtat tggcggattt acagatttct tctttaaagc
    19021 atgcttatcc agaagattta tccggtggtg aagaacaacg agtggcaatc gcaagagcct
    19081 tagttacagg aaagactctc atcttagctg atgaaccaac aagttctttg gataaagaaa
    19141 atagagaaaa aatctccgcc ttgttaattg atttagctca caaatatcat aaaattgtat
    19201 tgattgtttc gcatgatgag gcgatgattg caataggaga tgttcagctt cattttaaag
    19261 accataaatt agttgggaat tgtcttattt tgaattctga tgaagggata aaggattcta
    19321 agaagtggtc ctcctgtttg ttgtcttctc atacgaagct atccgttttg aaacatagaa
    19381 ggcctttttt acctagatta ttagcgtttt ttatctctct tgtagttgct atagcagtaa
    19441 cctctattaa ctttcagtcc ttatttgcag ataagtatca ccaactggtg aacaactcac
    19501 tcgaaaatgg cttcttggtg attaatgatt cgcttcattt acaaactaca aaagtgttgg
    19561 atgattttct cagttttgat aaggaggaaa tcacctcaat tagcacaagt cagaatattc
    19621 aaaccgtcaa tccttatata gagtttctct ctttgggtat gacgtttgat aattcaaaag
    19681 actattcaca acttcaaaag aactttcagc caactttgac tattgatggt cagaccagaa
    19741 gcttgacaag aaatttttca attcaaccct tgtatcagac aaatacaaca cagaggcaga
    19801 tagagtattt tgataggagc aatgaaaaag ggatttatct atctgagcaa ttcatttcag
    19861 atgaaaaatt accaaatata gtttcgggaa ctaccgtgac actgactttt tatattccaa
    19921 tttctttata tgaatcaagt atagagaaag aggggaatat attgaaagga gatggcggtt
    19981 tatttgtcaa agtggataaa acatatcctg ttttagggat tgttaaaaaa agttatccgt
    20041 ttgaatattc cccttatggg aacaccctat ttatgaatat tgctgaaatg gaagaattgc
    20101 aaaatgaagc aattcaacag catcccatga cgaagatggc tttagatggt tttcctttga
    20161 aatcatggat gccgagtgcg attcatgtgt tactaaagga tagcggtgct attcctgaag
    20221 aattgagtag aataagagca atctcttcac aaattactat tttatcatca tatgaaaact
    20281 acgaagaatt taataaagga ctcacctata tcaggcaatt tttaatgttg ctatccctag
    20341 tcttactcat ccttgtcatc gcagtattgt cctttgtttt ctttttgctc aatcgtcctc
    20401 gacgctttga agtagggatt ttaaaatctt tgggatacag tactcaaaat attgtttggc
    20461 ttttcttaaa agaattaatt ggttatggta aaacaatttc gatattagca agttgtttac
    20521 tcgtcattct ttccatttta gcaatgcaag tgcttaaatt ggaaatttca gatgttttta
    20581 aattctatct aacttccgtt ttcaccttaa ttggattatc agtctcagta ctgataataa
    20641 gtggcttact acctatatac acaacttgta ggcaaacagt tgtcgatact atccgaaaga
    20701 atggatgagg tgatatcatg aaacataaga taatgaaaaa aataatagtg gtagtgtttg
    20761 ctatcccatg gttaacattt ttaagtcaag tacatgcaga ttatgtaagt aagtctagtt
    20821 ctttagacgg ttatagcttg gtaggcttga atgttcttaa tgtgactttt aatgcttctg
    20881 gctattctga tacttatagt ggcagtgtgt caaatatgta tttcactgac tgggcttggt
    20941 ttcctaatac gataagtaat cgttccactt ggaaaagttc gattccatat ggtcaacgcg
    21001 caaatggtac agtccatata ggtgtaggag taaattcgcc ttggggagcg gttaatctct
    21061 ggggcggaca aaattacttt tctttagatt tttaacagaa ataatcaaag ttagatagaa
    21121 tgaataagaa tgctcttgat gatggtatca agagtatttt atgttattca gattaatgca
    21181 aacattgccc tttcttgtcg caggctccgt ttcatgatac aatagaagga gtgattttag
    21241 aaagcgtgag aaacatgatt tactttgata attcggcgac gaccaggccc tatccagaag
    21301 cccttgaaac ctatatgcag gtggcttcaa aaattttagg aaatccatct agtctccatc
    21361 gtttgggaga ccaggcaaca cgaattttag atgcttcccg tcaacagatt gcagatttaa
    21421 tcggtaagaa aagtgatgaa atcttcttta catctggtgg gacagaaggg gataactgga
    21481 tcatcaaggg agtagcattt gaaaaagccc agtttggcaa gcacatcatc gtatcagcca
    21541 ttgaacatcc agcagttaag gaatcagccc tctggttgaa agctcaaggt tttgaagtgg
    21601 attttgctcc agttgataac aaaggatttg tggatgttga ggcgctagaa gatttgataa
    21661 gacctgatac gaccctcgtt tccatcatgg cagttaacaa tgaaattggc tctgttcagc
    21721 ccattgaggc tatttcaaaa ctattggcag acaagccaac tatttccttc cacgttgatg
    21781 cggttcaggc gcttgccaag attccgactg aaaagtatct gacagaacga gtggatttcg
    21841 cgactttctc tggtcacaag ttccacggaa tccgtggtgt tggttttatc tatatcaagt
    21901 ctggcaagaa gattacgcct cttttaacag gtggtggtca ggaacgtgat taccgttcga
    21961 caactgaaaa tgtggcagga attgcagcga ccgctaaggc tctccgttta tctatggaaa
    22021 agctagatat ctttaggagc aagactgggc agatgaaggc agtgattcgt caagcacttc
    22081 tggactatcc ggatatattt gtcttttcag atgaggaaga cttcgcccct catattctga
    22141 cttttggaat caagggtgtt cgtggtgagg tcattgttca cgcctttgaa gactatgata
    22201 ttttcatctc aacgacctcg gcttgttcgt ccaaggctgg gaaaccagcc ggaaccctga
    22261 ttgctatggg agtggacaag gataaggctc agtcagctgt gcgtcttagt ctagaccttg
    22321 aaaatgatat gagtcaggtc gagcagttct tgaccaagct aaaattaatt tacaatcaaa
    22381 ctagaaaagt aagataggag cattcatgca gtattcagaa attatgattc gctacggtga
    22441 gctgtcaacc aagggcaaaa atcgtatgcg tttcatcaat aaacttcgca ataatatttc
    22501 agacgttttg tctatctatc cccaagttaa ggtaacagca gatcgcgacc gtgcccacgc
    22561 ttacctcaat ggagcagatt acacagcagt agcagaatcg ctcaaacaag tatttggaat
    22621 tcaaaacttt tcccctgttt ataaggttga aaaatctgta gaagttctga agtctgctgt
    22681 ccaagagatt atgcaggaca tctacaagga aggcatgacc tttaaaatct ctagcaagcg
    22741 tagcgaccac acctttgaat tggacagtcg tgaactcaac caaacgcttg gaggggctgt
    22801 attcgaagcc attccaaacg tacaagctca aatgaaaagt cctgacatca atcttcaggt
    22861 ggagatacgt gaagaagcag cctatctttc ttatgaaacc gttcgagggg caggtggatt
    22921 accagtggga acttctggta agggcatgct catgttgtca gggggaatcg attcccctgt
    22981 agctggctat ctagcgctta aacgaggggt agatatagag gcagttcact ttgccagccc
    23041 accttacacg agtcctggtg ctcttaaaaa agcccaagat ttgacccgta aattaaccaa
    23101 atttggggga aatatccagt ttatcgaagt tccttttaca gagattcaag aggaaatcaa
    23161 agccaaagcg ccagaagcct accttatgac cttgacgcgt cgttttatga tgcgtattac
    23221 tgaccgtatt cgtgaggtac gaaatggttt agttatcatc aatggggaaa gtctaggtca
    23281 agtagccagc caaacccttg aaagtatgaa ggctatcaat gctgttacca acactcccat
    23341 cattcgtcct gtggttacca tggacaagtt ggaaatcatt gacatcgccc aggaaatcga
    23401 tacctttgac atttcaatcc aaccgtttga agactgttgt accatttttg caccagatcg
    23461 tccaaaaaca aatcctaaaa ttaagaatgc ggagcagtac gaagcgcgta tggatgttga
    23521 aggcttggtt gagcgagcag tggctggaat catgattact gaaatcacac ctcaagccga
    23581 aaaagatgaa gttgatgact tgattgacaa tctgctctaa ttcagaaaat ccaaaagaat
    23641 agcgaaaatc agtaaaaaaa gttagttttt tctctaaaaa caggtaaaaa actaactttt
    23701 tttattttta tgatataatg atataaaatt ttgaatatag agagttttct gacaatgaat
    23761 caatcctact tttatctaaa aatgaaagaa cacaaactca aggttcctta tacaggtaag
    23821 gagcgccgtg tacgtattct tcttcctaaa gattatgaga aagatacaga ccgttcctat
    23881 cctgttgtat actttcatga cgggcaaaat gtttttaata gcaaagagtc tttcattgga
    23941 cattcatgga agattatccc agctatcaaa cgaaatccgg atatcagtcg catgattgtc
    24001 gttgctattg acaatgatgg tatggggcgg atgaatgagt atgcggcttg gaagttccaa
    24061 gaatctccta tcccagggca gcagtttggt ggtaagggtg tggagtatgc tgagtttgtc
    24121 atggaggtgg tcaagccttt tatcgatgag acctatcgta caaaagcaga ctgccagcat
    24181 acggctatga ttggttcctc actaggaggc aatattaccc agtttatcgg tttggaatac
    24241 caagaaaaaa ttggttgctt gggcgttttt tcatctgcaa actggctcca ccaaccgcta
    24301 tatcgagtgc cagaaactat cgcctgacca acgcatcttc atctatgtag gaacagaaga
    24361 agcagatgat acagacaaga ccttgatgga tggcaatatc aaacaagcct atatcgactc
    24421 gtcgctttgc tattaccatg atttgatagc agggggagta catctggata atcttgtgct
    24481 aaaagttcag tctggtgcca tccatagtga aatcccttgg tcagaaaatc taccagattg
    24541 tctgagattt tttgcagaaa aatggtaagt taagaaagga aaaaacgaaa tgcatattga
    24601 acatcttagc cactggagtg gtcatcctaa ccgtgaaatg taccttaacc gttatggaca
    24661 tggtgggatt ccagttgtgg tctttgcttc atcaggtggt agtcacaacg aatactatga
    24721 ttttggcatg attgatgcct gtgcttcctt tatcgaggaa ggccgtgtcc agttctttac
    24781 cctatctagt gtggatagtg agagctggtt ggctacttgg aaaaatgctc atgaccaagc
    24841 ggaaatgcac cgtgcctacg aacgttatgt gattgaggag gccattcttt tatcaagcac
    24901 aagacaggtt agtttgatgg catgatgacg acaggttgct ctatgggagc ctatcatgca
    24961 ctcaatttct tcctccagca tccagatgtc tttaccaaag tgattgctct cagtggtgtt
    25021 tacgacgcac gtttctttgt cggtgattac tacaacgatg atgctattta ccaaaactcg
    25081 ccagtagatt atatttggaa ccaaaacgat ggctggttta ttgaccgtta ccgtcaggca
    25141 gagattgtgc tgtgtacggg gcttggagcc tgggaacaag atggtttacc atccttttac
    25201 aagctcaaag aagcctttga ccagaaacaa attccagcct ggtttgctga atggggacat
    25261 gatgtcgccc acgactggga atggtggcgt aaacaaatgc cttatttcct cggtaatctc
    25321 tatttataaa aggagttacc tatgaattac cttgttattt ctccctacta tccacaaaac
    25381 tttcaacagt ttaccatcga actagctaat aaaggcatca cagtcttggg aattggtcaa
    25441 gagtcttacg agcaattgga tgagcccttg cgcaatagct tgaccgagta ttttcgtgtt
    25501 gataatcttg agaacataga tgaagtcaaa cgtgcagttg cttttctctt ttataaacat
    25561 ggtccaattg accgcatcga gtctcacaat gaatactggc ttgagctaga cgcaacactc
    25621 agagaacaat tcaatgtttt tggtgccaaa ccagaggatc tcaaaaagac gaaatataag
    25681 tctgaaatga agaaactttt caaaaaagca ggtgttcctg tggtacctgg agctgttatc
    25741 aagacggaag cagatgttga tcaagcagtg aaagaaatcg gtcttccaat gattgccaaa
    25801 cctgataatg gagtgggagc agccgcaacc tttaaacttg agacagaaga cgatatcaat
    25861 cacttcaagc aagaatggga ccattcaacc ctttatttct ttgaaaaatt tgtcacttcc
    25921 agcgaaatct gtacctttga cgggctcgtg gacaaggatg gaaagattgt cttctcaaca
    25981 acctttgact acgcctatac accgcttgac ctcatgattt ataagatgga caattcttat
    26041 tatgtgctca aggatatgga tcctaaactg cgcaagtatg gggaagcaat tgtcaaagaa
    26101 tttggtatga aagaacggtt tttccatatt gagttcttcc gtgaggggga cgattatatt
    26161 accatcgagt acaataaccg ccctgcaggt ggttttacca ttgatgttta taactttgct
    26221 cattccttgg acctttatcg tggctatgca gctattgtcg caggagagga gttcccggcg
    26281 tcagactttg aaactcagta ttgtttggct acttctcgcc gtgcaaatgc tcactatgtt
    26341 tattcagaag aggatttgct tgccaaatat agccagcagt tcaaggttaa aaaagtcatg
    26401 ccagctgcct tcgcggaact tcaaggagat tacctgtata tgctgacaac tccgagtcga
    26461 caagaaatgg atcagatgat tgctgatttc ggacaacgtc aagaataaga actatcggat
    26521 taaggaaatt cactccctta atccttttgt tttgtctggt aaaaaataag agcatcctaa
    26581 caaggtagct atcataaaac ttgttcgata actatttgaa gcaggattag gtggctagaa
    26641 attgaatttt aacatttcaa tttagtcata gtattgtgtt ttcgtatcct taaatcagct
    26701 aaaaggatcc atgacgacac ctatacgata tagttttcag gataccaaac cagtctatta
    26761 atactcaatg aaaatcaaag agcaaactag gaagctagcc gcaggttgct caaaacactg
    26821 ttttgaggtt gtggatagaa ctgacagagt cagtatcata tacctacggc aaggtgaagc
    26881 tgacgtagtt tgaagagatt ttcgaagagt ataaatctta ttgatgaact gcttgcagtc
    26941 tgagaaaaaa tgagcttgga tattatttcc aaactcactt aaagtcaatt tcaatccact
    27001 agaacaagcc tagtacagtt ccatcgcttt caacatccat gttgagagct gctggacgtt
    27061 ttggaagacc tggcatggtc ataacatcac cagttaaggc aacgatgaag cctgcaccta
    27121 attttggtac caattcacga atggtaattt caaagttttc tggtgctcca agcgcatttg
    27181 gattgtctga gaaactgtat tgagttttag ccatacaaat tggcaatttg ccccaaccat
    27241 tttgaacgat ttgagcaatt tgtgtttgag ctttcttctc aaagttcact ttgctaccac
    27301 gatagatttc agtgacaatt ttttcaatct tttcttggac agaaaggtca ttatcgtaca
    27361 aacgtttata gttagctgga ttttcagcaa ttgtcttaac aactgtttcg gcaagtgcta
    27421 ctccaccttc tgctccatca gcccagacac tagccaattc aactggtaca tcgattgagg
    27481 cacagagttc ttttaaggct gcaatttcag cttctgtatc agatacaaat tcgttaatag
    27541 ctacaactgc tggaataccg aacttacgga tattttcaac gtggcgtttc aagttagcaa
    27601 aacctgcacg aactgcatct acattttctt cagtcagagc gtctttagcc acaccaccat
    27661 tcatcttaag ggcacgaagg gttgcgacaa taacaactgc atctggagat gttggcaagt
    27721 ttggtgtctt gatatcaagg aatttctcag caccaaggtc cgcaccaaaa ccagcttcag
    27781 taacagtgta atcagccaag tgaagggctg ttgtcgtcgc caaaacagag ttacagccat
    27841 gagcgatatt ggcaaatgga ccaccgtgta caaaggcagg tgtaccgtaa attgtctgaa
    27901 ccaagtttgg cttaatagca tccttcaaaa tcaaagccaa ggcaccctca acctgcaaat
    27961 cacctacaga aacaggcgta cggtcatagc gataaccaat aacgatattc gccaaacgac
    28021 gtttcaagtc ctcgatgtcc gttgccaagc aaagaattgc catgatttct gaagcaactg
    28081 taatatcaaa accatcctca cgtggaatac cgtttagagg accaccaaga ccaacagtca
    28141 catggcggag cgcacggtcg ttcaagtcca caacgcgttt ccagaggata cgacgttgat
    28201 caattcccag ctcattccct tggtgcaagt ggttgtcaat caaggcagaa agggcattgt
    28261 tggcagttgt aatagcatgc atatctccag taaagtggag gttgatgtct tccattggca
    28321 gaacttgtgc atacccacca ccagcagcac cacccttgat ccccatgact ggaccaagag
    28381 acggttcgcg gatagcaatc atggttttct tgccaatctt gttcaaggca tccgcaagac
    28441 caatggtaag cgtcgacttt ccttcacctg caggtgttgg gttgatggca gtaaccaaga
    28501 tcaatttacc aactggattg ctctcaactg cacgaatttt atcaaagctg agtttagcct
    28561 tgtactttcc gtacaactcc aaatcgtcgt aagaaatacc aagtttctct acaacatcaa
    28621 caattggctt caactcaata ctctgtgcga tttcaatatc tgttttcatt caaaattcct
    28681 ctaacctctt atatgataat tcattatatc acaaaacaag atttttaaca tcctaaaact
    28741 ctctaaacgt tcgtaaatat ctctgttttt aaggctttta gagtcctttc ttaaatttta
    28801 tatggcttta tagtttgaaa ctatagtaaa tcttcgtttt taccaaaaat ttatcacttt
    28861 cattttactt accgcttatt tttgtgtaca atagtgctat gaaaatttta gttacatcgg
    28921 gcggtaccag tgaagctatc gatagcgtcc gctctatcac taaccattct acaggtcact
    28981 tggggaaaat tatcacagag actttgcttt ctgcagggca tgaagtttgt ttaattacga
    29041 caaaacgagc tctgaagcca gagcctcatc ctaacctaag tattcgagaa attaccaata
    29101 ccaaggacct tctaatagaa atgcaagaac gtgttcagga ttatcaggtc ttgatccact
    29161 caatggctgt ttctgactac actcctgttt atatgacagg gcttgaggaa gttcaggcta
    29221 gctccaatct aaaagaattt ttaagcaagc agaatcatca ggccaagatt tcttcaactg
    29281 atgaggttca ggttttgttc cttaaaaaga cacccaaaat catatcccta gtcaaggaat
    29341 ggaatcctac tattcatctg attggtttca aactgctggt tgatgttacc gaagatcatc
    29401 tggttgacat tgcacgaaaa agtcttatca agaatcaagc agatttaatc atcgcgaatg
    29461 acctgactca aatttcagca ggtcagcacc gagctatatt tgttgagaaa aatcagcttc
    29521 aaacagtcca gactaaagaa gaaattgcag aactcctcct tgaaaaaatt caagcctatc
    29581 attcttagaa aggaaaacta tggcaaacat tctcttggct gtaacgggtt caatcgcctc
    29641 ttataagtcg gcagatttag tcagttctct aaaaaaacaa ggccatcaag tcactgtctt
    29701 aatgactcag gctgctacag agtttatcca acctttgaca ctacaggtac tctcacagaa
    29761 tcctgtccac ttggatgtca tgaaagaacc ctatcctgat caggtcaatc atatcgaact
    29821 tggaaaaaaa gcagatttat ttatcgtggt acctgcaact gctaacacta ttgcaaaact
    29881 agctcacgga tttgcggaca acatggtaac cagtacagct ctagccctac caagtcatat
    29941 tcccaaacta atagctcctg ctatgaatac aaaaatgtat gaccatccag taactcagaa
    30001 taatctgaaa acattagaaa cctacggcta tcagctgatt gctcctaagg aatccctact
    30061 agcttgtgga gaccacggac gaggagcttt agctgacctc acaattattt tagaaagaat
    30121 aaaggaaact atcgatgaaa aaacgctcta atattgcacc cattgctatc ttttttgcta
    30181 ccatgctcgt gatacacttt ctgagctcac ttatctttaa cctttttcca tttccaatca
    30241 aaccgaccat tgttcatatt cctgtcatta ttgccagcat tatttatggt ccacgagttg
    30301 ggattacact tggatttttg atgggattac ttagcttgac ggttaacacg attacgattc
    30361 taccgacaag ctacctcttc tctcccttcg taccaaacgg aaacatctac tcagctatca
    30421 ttgccatcgt cccacgtatt ttgattggtt taactcctta cttagtctat aaactgatga
    30481 aaaacaagac tggtctgatt ttagctggag cccttggttc cttgacaaat actatctttg
    30541 tccttggagg aatcttcttc ctatttggaa atgtttataa tggaaatatc caacttcttc
    30601 tggcaaccgt tatctcaaca aattcaattg ctgaattggt catttctgca attctaaccc
    30661 tagccattgt tccacgacta caaaccttga aaaaataaaa acaatctccg ctttcaaacc
    30721 tgaaggtgga gattttgctc tatacagttt ctttttaatg aaatctttac tatagtaaaa
    30781 cgaaataaga acaggacaaa tcgatcagga cagtcaaata gatttctaac aatgttttag
    30841 aagtagaggt ggactattct agtttcaatc tactataact tttaactaaa aaatgatcta
    30901 taaacccaag taaattcttg ctttattgtc tggtttattg taccatacta gtgtatatgc
    30961 agttaaaaag gagattcttg tgaatacacg gaaaaagaca caatttatga caatgacagc
    31021 ccttttaacg gctattgcga ttttgattcc aattgttatg cctttcaaga ttgtcattcc
    31081 acctgcttcc tatactttgg ggagccacat cgctattttt atagccatgt tcttgtcgcc
    31141 cttgatggca gtttttgtca tcctagcctc tagttttgga tttttgatgg ctggctatcc
    31201 catggttatc gtttttcggg ctttttccca tatatctttt ggtgctttag gagctcttta
    31261 cctacaaaaa ttccccgata ccctagataa accaaaatct tcctgggttt tcaattttgt
    31321 tttggctgtt gttcatgccc ttgctgaaat attagcctgt gtcctttttt acgcaacttc
    31381 gggtaccaat gtagaaaaca tgttctatgt tctatttgta ctagttggat ttggcacaat
    31441 tatccatagt atggtagact atacattagc actagctgtc tataaagtgc ttcgaaaacg
    31501 ccgttaaaag gaaaagctat gacaaaagat cgcaaacaag ccctgctcca actcttgaaa
    31561 gaagctccta aagcccttaa tggccaaagt ttggcggaac attttcatgt cacacgtcag
    31621 gtcattgtgc aggacattgc aattttgaga gccgatggcg ctcctatcct atccaccaat
    31681 cgtggctaca tctataagca aattgatacc aatccttatg ttcacaaact tttcaaagtg
    31741 aaacatgaag ttgaagaaat cggtcaggaa ctccttgcta ttgtcgataa tggtgggcat
    31801 gtccagaata ctttgattga ccatcccgtt tatggagaaa ttgaaacctt gcttaaactg
    31861 tcttgccgcc gagacgttca acattttcta gaacaagtcg agcattctga ttttaaacct
    31921 ttatctgaat tgacagatgg tgtccattac cacctagtcg aagccgaaac acaacaagac
    31981 ctccactata tcgaggaggc cttggatcag ctaggttatt tagtaaagga ttagaaaatt
    32041 ttcttttccc aatcgtcttc tgtacggcga gggtagaaaa attcagattt gtccgatgct
    32101 tccatcatct ccatcaaagg tagcatatca taggccagat ccaagtttgg aatctggtct
    32161 ttttgcaccc aagaaacttc tccttcatct gaagagcgaa gggtaccaga gaactcagtt
    32221 gccttataac aaacgacaat atagcgccca cctgtatcta gtggccaatt tttaatgccg
    32281 acaagttgag gattttggat agtcaaccct gtttcttcat agatttcacg aatgacagac
    32341 tctgcgaaag attcgccatt ttctacatgg cctcctggaa aggcataacc agaccagcga
    32401 ttagtttcag gagcgcgata ctgcatcacc acgcgctgag cttcgagatc ttcaatcaga
    32461 caaatatttg ttaaaatcgt taattgtgct cgagacataa atttactcac tttctaattt
    32521 attaaatttt tagacttgat accagtacca ttagcttcta ctttttaaaa ttatttagaa
    32581 tagaatacca tccgtttttc ctttatgggc tttccaagtt caagtatctt ttcttgttca
    32641 tcaaaagtaa agcccatttt ttgatagaaa gcaattgcgc gcttattatc tttcaatacc
    32701 cataagaaaa tttcagaaaa atgattaaga gcaatcaaag ctgcttttat taacttttgt
    32761 gcgatacctt ttccataata gtcttttaaa acatataaag caataatttc gccagcttga
    32821 atagtctcat cacgaaagtt gccataactg ataaaaccaa ctaccttcat accatccatc
    32881 gcaatcaatg tattttctgg atacttttga ctaaagaatc gacatctttc taatgtcatt
    32941 gtctcctgaa attctgcagg caaaaggcca tcataagcct ctctccacgt ttgccagtga
    33001 acaaggggct taccttctat ctcttcagga gtttccattg atttgataat aaccgtcatt
    33061 tgttttctcc cagtcttctc tcaaaatacc atatttaata ctatcaaaat atttaccttg
    33121 ataataacga acttttggaa tatgagcttc ttttttcatt cttaattttt cagcaagttt
    33181 catcatacca agattccctg accaagttgt caaacccaga tgctccaact ccaagtaatc
    33241 ctgaaacgtc ctatctatcc actgcaacat agcaactttc ccaataccag tgttccagaa
    33301 ttttttatca taaataccaa ttcccaattc catccatctt gtttgtttac atacccaata
    33361 acgcgaaaca gtcccaacta gtttatcatc aacaaaaata ccaaggcgat ttgagttgct
    33421 taaaatggat tctgattttt gtagttcgaa ttctttaaaa tttgaaaaat actgataatc
    33481 atcataatag ggagcatcat actgtttcca aactggatta agttgtgaat aagccatttc
    33541 ccataaagac atcaaatctt ctctctttaa ctttcttaaa taaatcatac tattccccta
    33601 atccaaggcc ttgacttcca acatcatatc acggatctga gccgccagtt caaagtcaag
    33661 tacttcgacg gcttcttgca tttgtttttc tagtttcttg acgagttctt tgcgctcttg
    33721 tttgttgagg ctattgatgt cgacttcctt gtcctcttcc ttagcaactg ccttggtcac
    33781 agcaatcagg tcacggattt ctttcttgat tgtctgtggt acgataccat gctcttcatt
    33841 ataggccatc tgaattttcc gacggcgggc agtttcatcg atagcacgtt gcatagactg
    33901 ggtaaccgtg tctgcataca tgatgacatg gccttcactg ttacgagcag cacgtccaat
    33961 agtctggatg agtccacgtt cgttgcgaag gaaaccttcc ttgtcagcat cgagaatagc
    34021 tacgaggctc acttcaggaa cgtcaattcc ttcacggagc aggttaattc cgaccaagac
    34081 atcaaagaca cccaagcgca ggtcacggat aatctccgtc cgttccaagg tcttgatatc
    34141 cgagtgcatg tacttaacct tgatacccat ttccttgaag tagtcggtca agtcctctgc
    34201 cattttcttg gtcaaagttg tgataaaggt acgctcattt ttttcaacgc gggcattgat
    34261 ttcacccaag aggtcatcaa tctgtcccat agtcggacgg acttccacct ctggatccaa
    34321 gagtcccgtt ggacgaatga tttgctcaat cactgtctcg gtctgttcat tttcatagtc
    34381 accaggtgtc gctgaaacgt aaacaatctg atgaacgtga ctctcaaact cctcccgacg
    34441 gagaggacga ttgtccaaag cagacggcaa acggaaacca taattaacca gcatttcttt
    34501 acgcgaacgg tctccattgt acatgccctt gatttgccct atggtcatat gactctcgtc
    34561 aatcataatc aagaaatcat ctgggaagaa gtcgagaagc gtataaggag gctctccttc
    34621 gctccgtcca tccatgtggc gagaataatt ttcaacccca ttggtatagc ccatctcacg
    34681 caacatttcg atatcatact ctgtccgctg tttcaaacgc tgggcttcaa gcagtttacc
    34741 ttccttttca aagacagcta attgttcttc caactcggcc tgaatctttg caatggcaac
    34801 ttccatgtgg tcgtcattgg tcacaaagtg tgtcgctgga aaaatcgcta aatgatccac
    34861 ttctcccaac acctgacctg tcagagcctc aacttcacga atacggtcaa tttcgtctcc
    34921 aaaaaattct actcgaaagg catgttcatc tcgggaagct gggaaaatct ctaccacatc
    34981 cccacgaacg cgaaatcttc cgcgttggaa atcaatatca ttgcgttcaa actgaatatc
    35041 gaccaagtca ttcaagagtt tatcacgaga aatctctaga ccaggacgga gactaacgac
    35101 actatcagcg tattccttgg gcgaacccaa accatagata caagagactg aggccacgac
    35161 aataacatca ttacgctcca aaagggctga ggtagctgag tggcgaagct tgtcaatctc
    35221 gtcattgaca gaactatcct tctcaatata ggtatcgcta gaagggacat aggcctctgg
    35281 ctggtaataa tcatagtagg atacgaaata ctcaactgca ttttcaggga aaaattcctt
    35341 aaactcccca tagagctgac cagccagagt tttattgtgg gcaataacca gagttggttt
    35401 attgacttta gaaatgacct gactcatagt ataggtcttc cctgttccag tcgcccccat
    35461 cagaatctga gctttttctc ccccctcaat gttatccacc aactgctcga tagcttgggg
    35521 ttgatctcct gatggttgat attttgatac tagtttaaat tgattatctg taatgtgatt
    35581 gatcataaga atcctctctc catgtgctat tcctatttta gcatacttat agtattttca
    35641 cgaagaaaag gacggaccga agtcacatcc tttcattttc tttctttaat ttattagcaa
    35701 gatagacaat cagcagtagt aataccagac ttgtcataat ggaaccttct atgccaaaag
    35761 aaccacctga taaccaatct tgattgcctt gtggtaaaaa ggtcatcaga gacgttcctg
    35821 acggttgacc actaactaaa atcccaaaga gattaccctg agcaaaattc caagcaccat
    35881 gaatacctgc aacaccccaa actgtatcag ttttgagaag gtaaagagcc atggcaactc
    35941 cgaataaaaa gagatttact agagatagag gggtgaggcc agaattgccc atatgaagca
    36001 gggtaaagaa caggctagat ataagaatag ctagttttag attggttctt gaggccaatt
    36061 gaggaagtag ccaagcacgg gccaccactt cttctgctgt cccctgtaaa atccaaaatg
    36121 ggatagtaaa gacgacaaag gcaagagaat aaggattcaa gtgaatggat tccaaacgat
    36181 attgacctaa gaccaccaaa cctaacaagg tcagaagaaa aagtgccaga cctagactaa
    36241 atcctttcag aagattgctg aggcaattct ctctataaaa tcccaaggtt cgaataggtc
    36301 ttttctcaac ttttcttgtc catctgaaca cagtgttaag aataaaacca aaggccagta
    36361 atcctaaaat taaacgaaag tcacttgttt tatccgtcaa gctctgatgc aaggtctgca
    36421 agtaggttgc aagattttga ccagcctctc caaaatttct agcaagtccg atgagagcta
    36481 acaagccaat accatacaaa gtaaaagcca caaactctcc tatgaagaca aaaacaaagg
    36541 ctaacaaggg gcttgtgtaa atgtttaaac tcatttttga agcttggaag tctttaaata
    36601 ttcttttgtt cttcatatcc ccgtcctctt ttc
//
LOCUS       DAVKJJ010000018        35194 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000018 DAVKJJ010000000
VERSION     DAVKJJ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35194
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.018"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            <1..126
                     /locus_tag="VW144_000873"
     CDS             <1..126
                     /locus_tag="VW144_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU9600341.1"
                     /translation="NDYVSAEQFYQKSKLFADIIENSYLVTMLEKQWQEDLKKYL"
     gene            446..742
                     /locus_tag="VW144_000874"
     CDS             446..742
                     /locus_tag="VW144_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU9600342.1"
                     /translation="MKQTVKKLALVASIAATLGGSVAVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            819..2849
                     /locus_tag="VW144_000875"
     CDS             819..2849
                     /locus_tag="VW144_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9600343.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDYREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLAATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVHLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYIP
                     SGNVIYVSPRYLIEEKITVSSEFIDKMQNLSEGEFGLILPESLREQSAYYQGLFTDYL
                     QNFSSESVEVTSQKHYFPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            2851..3492
                     /locus_tag="VW144_000876"
     CDS             2851..3492
                     /locus_tag="VW144_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9600344.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            3485..3793
                     /locus_tag="VW144_000877"
     CDS             3485..3793
                     /locus_tag="VW144_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600345.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC
                     "
     gene            3924..4796
                     /gene="rsmA"
                     /locus_tag="VW144_000878"
     CDS             3924..4796
                     /gene="rsmA"
                     /locus_tag="VW144_000878"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU9600346.1"
                     /translation="MRIADYRVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILVDTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            4798..5676
                     /gene="rsgA"
                     /locus_tag="VW144_000879"
     CDS             4798..5676
                     /gene="rsgA"
                     /locus_tag="VW144_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU9600347.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            5687..6343
                     /locus_tag="VW144_000880"
     CDS             5687..6343
                     /locus_tag="VW144_000880"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU9600348.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            6306..6968
                     /locus_tag="VW144_000881"
     CDS             6306..6968
                     /locus_tag="VW144_000881"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEU9600349.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFEQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            6970..8226
                     /locus_tag="VW144_000882"
     CDS             6970..8226
                     /locus_tag="VW144_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU9600350.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            8216..9157
                     /locus_tag="VW144_000883"
     CDS             8216..9157
                     /locus_tag="VW144_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEU9600351.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            9424..10251
                     /gene="purR"
                     /locus_tag="VW144_000884"
     CDS             9424..10251
                     /gene="purR"
                     /locus_tag="VW144_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEU9600352.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            10318..11568
                     /locus_tag="VW144_000885"
     CDS             10318..11568
                     /locus_tag="VW144_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU9600353.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            11694..12488
                     /gene="pflA"
                     /locus_tag="VW144_000886"
     CDS             11694..12488
                     /gene="pflA"
                     /locus_tag="VW144_000886"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEU9600354.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(12563..13489)
                     /gene="yidC"
                     /locus_tag="VW144_000887"
     CDS             complement(12563..13489)
                     /gene="yidC"
                     /locus_tag="VW144_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9600355.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(13567..13845)
                     /locus_tag="VW144_000888"
     CDS             complement(13567..13845)
                     /locus_tag="VW144_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU9600356.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            13879..14619
                     /locus_tag="VW144_000889"
     CDS             13879..14619
                     /locus_tag="VW144_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU9600357.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRNQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTAEAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVATGILIFHLS"
     gene            14675..15358
                     /locus_tag="VW144_000890"
     CDS             14675..15358
                     /locus_tag="VW144_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU9600358.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            15469..16461
                     /locus_tag="VW144_000891"
     CDS             15469..16461
                     /locus_tag="VW144_000891"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEU9600359.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            16526..17065
                     /gene="rsmD"
                     /locus_tag="VW144_000892"
     CDS             16526..17065
                     /gene="rsmD"
                     /locus_tag="VW144_000892"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU9600360.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAKRELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            17055..17543
                     /gene="coaD"
                     /locus_tag="VW144_000893"
     CDS             17055..17543
                     /gene="coaD"
                     /locus_tag="VW144_000893"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU9600361.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACHVPESILEEIRNEK
                     KD"
     gene            17527..18564
                     /locus_tag="VW144_000894"
     CDS             17527..18564
                     /locus_tag="VW144_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU9600362.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKEILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            18622..18852
                     /locus_tag="VW144_000895"
     CDS             18622..18852
                     /locus_tag="VW144_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEU9600363.1"
                     /translation="MVQLLFTLSSHMFFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            18876..20159
                     /gene="murA"
                     /locus_tag="VW144_000896"
     CDS             18876..20159
                     /gene="murA"
                     /locus_tag="VW144_000896"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9600364.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            20152..20340
                     /locus_tag="VW144_000897"
     CDS             20152..20340
                     /locus_tag="VW144_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9600365.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            20379..21203
                     /gene="endA"
                     /locus_tag="VW144_000898"
     CDS             20379..21203
                     /gene="endA"
                     /locus_tag="VW144_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEU9600366.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            21494..22825
                     /locus_tag="VW144_000899"
     CDS             21494..22825
                     /locus_tag="VW144_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEU9600367.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(23023..23093)
                     /locus_tag="VW144_000900"
     tRNA            complement(23023..23093)
                     /locus_tag="VW144_000900"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23059..23061),aa:Cys,seq:gca)
     gene            23509..27120
                     /gene="rpoB"
                     /locus_tag="VW144_000901"
     CDS             23509..27120
                     /gene="rpoB"
                     /locus_tag="VW144_000901"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9600368.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            27153..30830
                     /gene="rpoC"
                     /locus_tag="VW144_000902"
     CDS             27153..30830
                     /gene="rpoC"
                     /locus_tag="VW144_000902"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU9600369.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYIGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            30941..31354
                     /gene="ndk"
                     /locus_tag="VW144_000903"
     CDS             30941..31354
                     /gene="ndk"
                     /locus_tag="VW144_000903"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU9600370.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(31864..32142)
                     /locus_tag="VW144_000904"
     CDS             complement(31864..32142)
                     /locus_tag="VW144_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HEU9600371.1"
                     /translation="MKKFDNYIIEKPCDSNSDKLQKILIIENLVDDILQFPLRINNSV
                     GEIFLLQPFQKKTIFIPCYFEEDIVKVKDDDKVEWNLLEFQKFRAFLA"
     gene            complement(32283..32915)
                     /locus_tag="VW144_000905"
     CDS             complement(32283..32915)
                     /locus_tag="VW144_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9600372.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(32917..34932)
                     /locus_tag="VW144_000906"
     CDS             complement(32917..34932)
                     /locus_tag="VW144_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9600373.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSHYYPNVVVRDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKNLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMHLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFVALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEVWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLTTSTEWHDY
                     SPNGNVLIVTPHYLERQNIPVDTTIKQKMNHLNVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSILVLKGG"
BASE COUNT        10267 a         6881 c         8012 g        10034 t
ORIGIN      
        1 aatgattatg tttctgccga acagttctat cagaaatcga agctatttgc tgatataata
       61 gaaaattctt atttagttac tatgttagaa aaacaatggc aagaagattt aaaaaaatat
      121 ttataaacat agtgaatcag tgacaaaaat gtccttgtcc tcgtatcaaa acagttctaa
      181 agttcgtctt tagaactgtt tttttagata taagctaaaa ataatatgaa atagttagat
      241 tttaaggaca ttgatgtcct tgatagtgtg ataaagatgg agtataatga ctataaaagt
      301 tcatttcagg gtgctatttt gacaatttag agaaaaacaa ataattttag aatatcattt
      361 ggtaatataa tagtatcaac gttgataatg gaaagaggag caagtaactc aacaatcttg
      421 taatcaaaaa tagaggagta tcaatatgaa acaaacagtt aaaaaattag ctcttgtagc
      481 gagcattgca gcaacattag gtggaagtgt agcagttgcc tctgcagccg ttcaataccc
      541 agaaggtggt gtttggactt atggttcagg taacggtggt gcttactcaa actactatca
      601 cccttcaaaa taccatagtt caacagttgt tagcagaaaa actggttcat ctgacaaggg
      661 atatgctggt gctggaggga cttctcgtgc atggattcgt acttcttggg gagagaaagt
      721 tgcattctac tataatgttt agaatggtat agccctattg tttagcttta cttggtcacc
      781 ttctcactaa gtgaccaagt aactttttgg aggaaatgat gaaacgttta tttattttga
      841 tttcaatggt attggtatcg ctttatatgg tgataacttc cgttgactat cgagaagaga
      901 ttttatttgg taactatcct tctgttgatg tgacaggaat gatgataaat caaccagtag
      961 ctagtcgcga agaggtgaca gaggctttga gtcacttggc ggtagagcat aatagtctca
     1021 tcgcccgtcg aatcgttgag ccaaatgaag ctggagaaac acgctttacc tatgccactt
     1081 atggtgaggg aaagcttcca gaaggtctga ccatttcctc caaggagagt gcagaaacga
     1141 gtgatttatt agggtcttac ttgattgtgt caggaagttt ggatggagtg agcttacaga
     1201 ccaccttgaa agaacttggc tatcaaggct ttgtttcgaa tggagaagat ccattttcga
     1261 tagtcttact attggcggcc acccctatgg tgctactgag tttagctatt tttctgctga
     1321 cctttatgag tctgaccctg atttatcgga tcaaatccct tcgtcaggca gggattcgct
     1381 taatagctgg tgagagcttg tttggagttg ctctcagacc agtgttagaa gatgtgagac
     1441 agcttatctg ctcagtgctg gtatccagtc ttttgggatt ggggattctc tggtatcaag
     1501 gtgccttgtt tatggcaacg gtacaactgg tcatcattgc tcttctactt tatggattga
     1561 ccctggcagg gatttctacc ttactaagtg tcgtccatct acttggttta caggaaaata
     1621 gtctggtgga tctattgaaa gggaaactcc ctctcaaacg gatgatgaca ttgatgatgg
     1681 tggggcaact cttagctgta ttggtggtcg gatcgagtgc gacagctctc ctaccccact
     1741 accgtgaaat gcaggaaatg gagagagcta gcaataaatg gagccagtcc tcagaccgtt
     1801 accgtctatc ctttggttgg tctagtgcat ttgccgatga agaaggaacg cgtaaggata
     1861 atcgtgagtg gcagacattt actgaagaac ggttagccaa tacagactct ttttatatta
     1921 tgagcaatgt tgacaatttc tcagatggag cagaagtgga cctagatggc aatcgtctca
     1981 gtgactacat accgtcaggg aatgttatct atgtctcgcc gcgctatctg atagaagaaa
     2041 agattaccgt ttcttcagag tttattgaca agatgcaaaa cttgtctgag ggagagtttg
     2101 ggctgatctt gcctgagagc ttgcgagagc agtctgccta ctaccaagga ttgtttacag
     2161 attacctgca aaacttttca tctgaaagtg tagaagtgac gagtcagaaa cactacttcc
     2221 cacaggtaag gctagctttt acagaaacag gacaggaacg tttcctctat aatgatgggt
     2281 acaagacaac acgccagtac ctaaaagatc cgattattgt agttctaacg ccgcaagcga
     2341 ctggaacaag acctgttgca gggatgttgt ggggaactac ggctaatagt gccttgaaac
     2401 tagatcgata tggagacagc atcacagctc taaaagagca aggtctgtat cacaaggttt
     2461 cttacttggt aaaaagccag ctattttttg ccaaggtact aaatgacaaa cgggtggagt
     2521 tttactctct ccttattggg acgattttga ccctgtctac ggctatcttg ttatttgatt
     2581 ccatgaatct tctctatttt gagcagttca gacgggagct tatgattaaa cgtcttgctg
     2641 gtatgacaat ctatgagctt catggcaagt atttactggc gcaaggagga gttctcttgc
     2701 ttggcctagt cctatctagt attttgacaa gagatggttt gattagcgct ctagttgtag
     2761 ctttgtttac gcttaacgcc ctcttgattt tagtaaggca ggacaaaaaa gaagaagctg
     2821 gtagcatggc agtattgaaa ggaaaataag atgattgata ttcaaggatt ggaaaagaaa
     2881 tttaatgacc gcgcgatttt ctctggtttg aatctcaagc tggagaaggg caaggtttat
     2941 gccttaatcg gaaagagtgg aagcggaaag acgacgctgc tgaatatctt gggaaagcta
     3001 gaaaagatag atggaggaag ggttctctat caggggaaag atttaaaaac cattcccact
     3061 cgtgagtatt ttcgagatca gatgggctat ctctttcaaa attttggcct cttagaaaac
     3121 caatcaatca aagaaaattt ggatttgggt tttgttggtc agaaaatctc aaaagtagaa
     3181 cgtttggaaa ggcaagtggg ggctttagaa aaagttaatc tagggtattt ggatttagaa
     3241 caaaaaattt atactttatc tgggggagag gcccaacgag ttgcccttgc taagactatt
     3301 ttgaaaaatc cacccttgat tttggcagat gaaccaacag cagctcttga tcctgaaaat
     3361 tcagaggagg ttatgaatct cttggtggat ttgaaagatg aaaatcgaat tatcatcatt
     3421 gcgacccata atcccctagt ctggaataag gctgatgaaa tcattgatat gaggaaactt
     3481 gctcatgtgt gaaaaaatcc gtattcgcag ggtatctgat tatcctagtg ccagaggtgg
     3541 tttagaagat atcctcatca tggaaaatat gaccaatcat ctccttttgg ttcaaatccg
     3601 agtgcatggc tatttgcttg attttgctag tattgaaggg caaaggcaaa agcattatcg
     3661 tttgaaaaat ttacctcaga cggttgaact gacagtggat gatgtggagg aggatgtgga
     3721 tttgacccta cctgaaaatc gaagttatca agaagctgat ttttttgaac gcatgtttcg
     3781 agagaactgt taaggccact tttaaagatt tccaagacta tctttcttca tgaggaaaga
     3841 tagttttttg gtatgatttt cattcccaaa atacaagggg aatgtgttac aatagtagta
     3901 acagataata gaaaagagaa tagatgagaa ttgcagatta tagagtgacc aaggcagtgc
     3961 tggagcgtca cggttttacc tttaaaaagt ccttcgggca aaatttcctg acggatacca
     4021 atatccttca aaaaattgtg gatacggctg aaattgatga ccaggtcaat gtcatcgaaa
     4081 tcgggccagg tattggtgcc ttgacggagt ttctggctga gcgtgcagcc caagtcatgg
     4141 cttttgagat tgaccaccgt ttggtgccaa ttttggtaga taccctgcgt gattttgata
     4201 atgtgactgt agttaacgaa gatattctca aggttgattt ggcgcaacat atccaaaatt
     4261 ttaaaaatcc taacctgcca atcaaggtag tggctaattt gccttactac atcacgacgc
     4321 ctattctcat gcacttgatt gagagtggca ttcctttttg tgagtttgtg gtcatgatgc
     4381 agaaagaagt agcggaccgc atttcagccc agcctaacac caaggcttac ggtagcttgt
     4441 ctatcgccgt gcagtattac atgacagcca aggttgcctt tatcgtgcct cgtacggtct
     4501 ttgtgccagc gccaaatgtg gattcagcca tcttgaaaat ggtgcgtcgt ccagagccag
     4561 ccgtagcagt agaagatgag aactttttct ttaaggtttc caaggctagt tttacccatc
     4621 gccgcaagac cttgtggaat aacttgacag gttactttgg taagactgaa gaggtcaagg
     4681 acaagctgac caaggctttg gaccaggcag gcttgtcacc aagtgtgcgt ggggaagctc
     4741 tcagcttggc agaatttgcc ggtctagcag acgcacttaa agggcaagga ctctaagatg
     4801 cagggacaaa tcattaaagc cttggcaggt ttctactatg tggagagtga tggccaggtt
     4861 tatcaaacac gcgcgcgtgg gaatttccgt aaaaaaggcc atacccctta tgttggggac
     4921 tgggtagatt tctctgccga ggaaaattca gaaggctata tcctcaaaat tcacgaacgg
     4981 aaaaacagtc tggttcgtcc gcctattgtc aatatcgatc aagctgtagt aatcatgtcc
     5041 gtcaaggaac ctgattttaa cagcaatttg ctggatcgtt tcttggttct tttggagcac
     5101 aagggcatcc atcccattgt ctatatttcc aaaatggatt tgttggaaga taggggagaa
     5161 ctggattttt accagcagac ctatggtgac atcggctatg actttgtgac cagtaaagag
     5221 gaactcctgt ctttgttaac aggcaaggtt acggtcttta tggggcagac aggtgttggg
     5281 aagtcaactc ttctcaataa aatcgcacca gacctcaatc ttgaaacggg agaaatttca
     5341 gacagtctag gtcgcggtcg ccataccact cgagctgtta gtttttacaa tctcaacggg
     5401 ggtaaaatcg cagatacacc aggattttca tccttggact atgaagtatc aagggctgaa
     5461 gacctcaatc aggctttccc agagattgct actgttagcc gagattgtaa gttccgtact
     5521 tgtacccata cccatgagcc gtcttgtgcc gtcaaaccag ctgttgaaga gggtgttatt
     5581 gcaaccttcc gttttgacaa ttacctgcaa ttccttagtg aaattgaaaa tcgtagagaa
     5641 acctataaaa aagtcagcaa aaaaattcca aaataaggag aaacctatgt ctcaatacaa
     5701 gattgctccg tcaattctgg cagcagatta tgccaacttt gaacgtgaaa tcaaacgtct
     5761 agaagcaact ggggcagaat atgcccatat cgatatcatg gacagtcatt ttgtaccgca
     5821 aatcagtttt ggtgcaggtg tggtcgagag ccttcgtcct catagtaaga tggttttcga
     5881 ttgccacttg atggtgtcaa accctgagca tcatctggaa gattttgcgc gtgcaggtgc
     5941 agacatcatc agtatccatg tagaagcaac acctcatatt catggcgccc tccaaaaaat
     6001 tcgttcactc ggagttaagc cttcagtcgt tatcaatcct ggcacaccag ttgaagccat
     6061 caagcacgtc cttcatctag ttgaccaagt tttagtcatg acggttaatc caggttttgg
     6121 tgggcaagcc tttctgccag aaaccatgga taaggttcgt gagttggttg ctcttcgtga
     6181 ggaaaaaggt ttgaactttg aaatcgaagt ggatggtggg attgatgacc aaactattgc
     6241 tcaagccaaa gaagctggtg cgactgtttt tgtagcaggt tcctatgtct ttaagggaga
     6301 agtcaatgag cgagtacaaa ctctcagaaa acaactggac tagggttgca gtttttgcag
     6361 gcggaaaccg cggtcattat cggacagatt ttgatgcttt tgttggggtg gatcgaggct
     6421 cgctctgggt cttggaagaa gacttacctc ttgctctagc agtcggagat tttgattctg
     6481 tgacggaaga agagcgacag gtgattcaaa aacgtgccca gtattttgtc caagcccgac
     6541 cagaaaagga tgatacagat ctggaattgg ctctcttaac catctttgaa caaaatcctc
     6601 aggctcaggt cactattttc ggtgccttgg gtggccgtat tgaccatatg ttggccaatg
     6661 tctttctacc tagcaatcct aagttggcac cctatatgcg tcaaatagaa attgaggatg
     6721 ggcaaaattt gattgcctat tgttcagaag ggaccagtca gctagaacct cgttcagact
     6781 acgactatct agcctttatg ccagttcggg atagccagct gactattctt ggagctaagt
     6841 atgagttgac agaggagaat tttttcttta aaaaagtgta cgcttctaac gaatatatag
     6901 atagggaagt gtcggtaact tgcccagatg gttatgtggt cgtactgcat agcaaggaca
     6961 ggaggtagga tggaaagttt acttattcta ttattaattg ccaacctagc tggtctcttt
     7021 ctgatttggc aaaggcagga taggcaggag aaacacttaa gtaagagctt ggaggatcag
     7081 gcagatcatt tgtcagacca gttggattac cgctttgacc aagccagaca agccagccag
     7141 ttagaccaaa aagatttgga agtggctgtc agcgaccgtt tgcaagaagt gcggattgaa
     7201 ttgcaccaag gcctgaccca agtccgtcaa gaaatgacag ataatctcct ccaaactaga
     7261 gacaagacag accaacgtct ccaagccttg caggaatcaa atgagcaacg tttggaacaa
     7321 atgcgccaga cggtcgagga aaaactagaa aagaccttgc agacacgctt acaggcttcc
     7381 tttgaaacag tttctaaaca actggagtct gtcaatcgtg gccttggaga aatgcagaca
     7441 gttgcccgtg atgtcggagc tcttaacaag gttctctctg gaaccaagac gcgaggtatt
     7501 ctgggagaat tgcaactggg gcaaattatt gaagacatca tgacacctgc ccagtacgaa
     7561 cgagaatacg caacggttga aaactctagt gaacgagtgg agtatgccat caagttaccc
     7621 ggacaaggcg accaagaata cgtctatctg ccaattgact ctaagtttcc actggcagat
     7681 tattaccgct tggaagaagc ctatgagaca ggtgacaagg atgagattga acgctgtcgt
     7741 aagtcactcc tagcaagcgt caagcgcttt gccaaggata tcaagagcaa gtacatagca
     7801 ccgcctcgga caaccaattt tggaatcttg tttgttccga cagaaggtct ttattcagag
     7861 attgtgcgca atccggtctt ctttgatgat ttgagacggg aagaacagat tatcgttgca
     7921 ggaccaagta ccctatcagc ccttcttaac tccctatcag ttggtttcaa gacccttaat
     7981 atccaaaaga gtgccgacca tatcagcaag actcttgcca gtgtcaagac cgagtttggc
     8041 aagtttggtg gtattctggt caaggcacaa aaacatctcc aacatgcctc tggcaatatt
     8101 gatgaattat taaaccgtcg taccatagct atcgagcgga cgctccgtca cattgagttg
     8161 tcagaaggtg agcctgcgct tgatctactc cattttcaag aaaatgagga agaatatgaa
     8221 gattagtcac atgaaaaaag atgagttatt tgaaggcttt tacctaatca aatcagctga
     8281 cctgaggcaa actcgagctg ggaaaaacta cctagccttt accttccaag atgatagtgg
     8341 cgagattgat gggaagctct gggatgccca acctcataac attgaggcct ttaccgcagg
     8401 taaggttgtc cacatgaaag gacgccgaga agtttataac aatacccctc aagtcaatca
     8461 aattactctc cgcctgcctc aagctggtga acccaatgac ccagctgatt tcaaggtcaa
     8521 gtcaccagtt gatgtcaagg aaattcgtga ctacatgtcg caaatgattt tcaaaattga
     8581 aaatcctgtc tggcaacgga ttgtccgaaa tctctacacc aagtatgata aggaattcta
     8641 ctcctatcca gctgccaaga ccaaccacca tgcctttgaa acgggcttgg cctatcatac
     8701 ggcgaccatg gtgcgtttgg cagacgctat tagcgaagtt tatcctcagc tcaataagag
     8761 cctgctctat gcggggatta tgctgcatga cttagctaag gttatcgagt tgacggggcc
     8821 agaccagaca gagtacacag tgcgaggtaa tcttcttgga catatcgctc tcattgatag
     8881 cgaaattacc aagacagtta tggaactcgg catcgatgat accaaggaag aagtcgtttt
     8941 gcttcgtcat gtcatcctca gtcaccacgg cttgcttgaa tatggaagtc cagtccgtcc
     9001 acgtattatg gaggcagaga ttatccatat gattgacaat ctggatgcaa gcatgatgat
     9061 gatgtcaaca gctctggctt tggtggataa aggagagatg accaataaaa tcttcgctat
     9121 ggataatcgt tccttctata aaccagattt agattgataa tttaagaaaa atgagcattt
     9181 tttaggataa gaatgttcgt ttttttatgt gaatatggta taataagtaa aagacaaaaa
     9241 tgaatactct tcgaaaatct cttcaaacta ggatagtatc gccttgtcgt atgtatatat
     9301 gcaggtatat tacagggttt gtcagttcta ttgacaatct caaaacagtg ttttgaacca
     9361 ccagcgacca gctttctagt ttgctttttg attttttgaa taaaaatgga ataggaaata
     9421 gaaatgaaat taagaagaag tgatcggatg gttgtcattt ccaactattt gattaataat
     9481 ccttataaac taactagtct caatactttt gctgaaaagt atgagtctgc taaatcatcc
     9541 atctcagaag atatcgtcat tatcaaacgc gcctttgagg aaattgaaat cggtcatatc
     9601 cagacagtga ctggggctgg cggaggtgtc atcttcacac cgtctatttc gagtcaggat
     9661 gctaaggaaa tggttgaaga cttgcgtacc aagttgtcag aaagtgaccg tatcttgcca
     9721 ggtggttata tctatctgtc tgatttgctt agcacaccag ccatcttgaa aaatattggt
     9781 cgtattattg ccaaaagctt tatggaccaa aaaattgacg cggttatgac cgtagcaact
     9841 aagggtgtgc cacttgcaaa tgcagttgcc aatgtcctca atgtctcttt tgtcattgtg
     9901 cgccgtgacc tgaaaattac cgaaggttca actgttagcg tcaactatgt ttcaggttca
     9961 agtggtgacc gtatcgagaa aatgttcctt tcaaaacgta gtcttaaggc aggcagccgt
    10021 gtcttgattg tggatgactt cttgaaaggt ggcggaacgg tcaatggtat gattagtctc
    10081 ttgcgcgagt tcgactcaga actggcaggt gtagcggtct ttgcggacaa tgcccaagaa
    10141 gaacgtgaaa agcagtttga ctacaagtca ctcttgaagg taaccaatat tgatgtcaag
    10201 aaccaagcca tcgatgttga ggttggcaat atctttgacg aagataaata agagatagaa
    10261 ctaaaggttg gaacgattgt cccagccttt ctttgcaaac agaatagaag gaagcttatg
    10321 aaaacaccat ttatcaatag agaagagtta gaagcgattg ttgccgagtt cccgactccc
    10381 tttcacttgt atgatgagaa ggggattcgt gagaaggcaa gagccgtcaa ccaagctttt
    10441 tcgtggaaca agggctttaa ggaatatttt gcagttaagg ctactccaac tccagctatt
    10501 ttgaaaattc tccaagaaga aggttgtggt gtggactgct ctagttatgt agagcttttg
    10561 atgagccata aactggactt tctgggttct gagattatgt tctcttccaa caacacgcca
    10621 gacaaggaat acgcctatgc acgtgaattg ggtgcgacca ttaacttgga tgcctttgaa
    10681 gatattgaac atctggagag agtagcaggc attccagaaa tcatctcttg tcgttataat
    10741 cctggaggcg tttttgaact ggggacagac attatggaca atcctgggga ggctaagttt
    10801 ggcatgacca aggaccagct ctttgaagcc tttgctatct tgaaggaaaa aggagccaag
    10861 acttttggga ttcactcctt cctagcgtcc aataccgtga cccatctcta ttatccagag
    10921 ttggctcgtc agctctttga actggctgtt gaaatcaagg aaaagttggg catttcgcta
    10981 gactttatca atctttctgg cggtattggt gttaattatc gtccagacca ggagccgaac
    11041 gatatcgcct tgattggtga gggagttcgt aaggtgtatg aagaagttct tacgtcagca
    11101 ggtcttggtc aggtcaagat tttcaccgaa ttgggtcgtt ttatgctggc acctcacggt
    11161 gctctagtca caagagtgac tcataagaag gaaacctacc gtacctatct aggtgtggat
    11221 gcctcagcag tcaacctcat gcgtccagct atgtacggag cttaccatca tattagcaac
    11281 gtgacccatc cagatggacc agctgaagcg gtagatgtgg ttggttcact ctgtgaaaac
    11341 aatgataaat ttgcagtgaa tcgcgaactg cctcatacag aaatcggtga tttgctggta
    11401 attcatgata caggtgccca cggattttca atgggctacc agtataatgc caaattacgt
    11461 tctgcggaaa tcctctatac cgaagaaggt aaagcccgtc aaatccgccg tgcagagcgc
    11521 cctgaggact attttgcaac cttatatggc ttcgattttg aagaataatc tgataataga
    11581 ttgaaaatga aattgaaaaa cagattgctt tctaaaaaat aggcaaaaat cttgtttttc
    11641 cttcaagtcg tgatataata aaactataaa acgttttcaa ggaaggtaac gatatgtctg
    11701 aagaaacaat tgattatgga caagtgacag gaatggtgca ttcgacagaa agctttgggt
    11761 cagtagatgg gcctggtatt cgctttattg tctttttgca gggctgtcac atgcgttgcc
    11821 agtattgcca caacccagac acttgggcta tggagtccaa taagtcacgt gaacggacag
    11881 tagatgatgt cttgacagag gccttgcgct accgtggttt ctggggaaat aagggtggga
    11941 ttacagtcag tggaggagaa gctctcttgc agattgattt cctgattgct ctcttcacca
    12001 aggctaagga acaaggaatc cactgtacct tggatacctg tgctcttcct ttccgtaata
    12061 aaccacgtta ccttgagaag tttgacaaac tcatggctgt cactgacttg gttcttttgg
    12121 atatcaagga aatcaacgaa gaacagcaca agattgtcac tagccaaact aataaaaata
    12181 tcttggcttg tgcccagtat ctatcagata ttggaaaacc tgtctggatt cgccacgtgc
    12241 tagttccagg attgacagac agagatgatg acttgattga acttggtaag ttcgtcaaga
    12301 ccctcaaaaa tgttgataag tttgaaattc taccttatca caccatgggt gagttcaagt
    12361 ggcgtgaact tggaattcca tattccctcg aaggagtcaa accaccaaca gcagatcgcg
    12421 tcaagaacgc taaaaaactc atggataccg aaagttatca agattatatg aaacgtgtac
    12481 atggatagaa aagaagcctg atggaaacat cgggcttttg atttgcaaaa agacttagca
    12541 aatcagctaa gcctttttct tcttatctcg aacgttgttt tccagcgttg cgatttttgt
    12601 gttttttctt gcttgtgata gcagttggtt gttcaggggt aacgtctttt cgtccacttg
    12661 gtttagagaa agcacttgct tttggtgggt tcttggctag ttcttcacgg acttttttgc
    12721 gaagttttgg acgaacgata tagttgacga taaactgttg gagaatcatc atgaaaccac
    12781 cgacaaccca gtaaagtgtg acactagctg gtgagaagag ggagaagacg acgatcatga
    12841 gtgggctcat gtaaatcatt ttcttgattt gttctctttg catttcatct tctactccgt
    12901 gaagtgaaag gagcgattga agatagtaaa ggacaccagc acaggcaacc aaaatcatac
    12961 ttggagaacc tagaggaatg cctaggtagc ttgcttgagc aaccccttca gtatgttggg
    13021 cagcaaagta gatagcagag aagaaaggca tttgaaggag gatagggaaa catcctacac
    13081 cgccaaacat gctgataccg tgctcttttt gagcggcaaa gagggcttgt tgggcttcga
    13141 gtttttcttc ttgagtagtt gcttctttga gacgtgtttg gtgtggctca aggacatgct
    13201 tgagagcgtt catcttttca gagtgaagcg ttgccttcca tgattggtag ataccaagtg
    13261 gtaagataat caagcgtacg ataatggtta cgataatgat agcgacacca aagcctagac
    13321 ctttatcagt agcgaagtac ttgatggctt cagccatagg cgctccgatc gtattccaaa
    13381 taaatcctgt tggctgacct gtggttttat cgacattgac acagccagtc aagacaagca
    13441 acatagccac tcccatagcc gagaatgcaa aacgtttaat agatttcact gtttttattc
    13501 cttctttaaa aattatacct ttctattcta ctgttttttt acaaaatata caatagttct
    13561 ggagacctaa tttgcgattt taaagtcaga gtagggagga aagttgctta gtttgacatc
    13621 taagtagctt acttttgaaa aaggtgtcgg tccttttcgg atttcttgga taaattttgc
    13681 catgatagca gatgagtctg cttgggctaa gatttccact gtgccatcgt cgttattcca
    13741 tacccgacct gtgatgccac cgatttcaag ggccaggcta taaacacccc aacgaaagcc
    13801 gactccctgc accctacctt gggcaatcat tctaaccttt tgcataccaa acccctttga
    13861 ttgtgttata atatttctat gactattata acctcaaaag ccaattctgt ggtaaaaaat
    13921 gctaagaaat tacaccaaaa aaaataccgc aagtctgcct atttgattga aggctggcac
    13981 ttgtttgaag aagctgtcca agctggagtg acgattgaga aaatctttgc cctagaaaat
    14041 tatcgaaatc agttagctgc ttttccgcaa actgtctgga tttcagagga tattttgcta
    14101 gatttggcgg attctcagac tccacaggga attgttgccg tggttcaaaa agaagaagta
    14161 ggacaagctg atttgagtca gggcaagttc ttgtttttgg aagatgtgca agatcctggt
    14221 aatgtaggaa ctatcattcg aactgcggag gcagcaggtt ttacaggagt gattgtttca
    14281 gataagtcgg cagacatcta cagtctcaaa accctacgtt ccatgcaagg tagtcatttc
    14341 catctgccca tttaccggat gactagtcaa gcgcttcttg acgaaactaa aaaggtagct
    14401 atcccagtgc tagcaacaac cctatctaaa gattctgttg attacagaga actgccttct
    14461 atagaaaatt ttgtactagt tatgggaaat gagggtcaag gaattagtcc ccttatggct
    14521 gaaagtgcag accagttggt ccatattagc atgaaggggc aggccgagag tttgaatgtt
    14581 gcagttgcaa ccggtatttt aatcttccat ttaagctaat tttaactttt tttgttataa
    14641 tcaaggaaag atgttcacag aaaaggagaa aaggatgaat cacactatta tacatgaccg
    14701 tgcaggtctc aatcaatttt acgctaaggt ttatgccttt gttggtctgg gaatcggact
    14761 atctgctttg gtatcaggcc ttatgttgac ggtctttcag tctcagttgg tttacttgtt
    14821 gatgcagggg cgtctctggt tgaccattgc tacttttgcg gagttggctt tggttttcgt
    14881 tgccagtagc atggcttcaa gaaatagtcc agctgctctt ccagtatttt tactttactc
    14941 cgttttaaac ggctttaccc ttagttttgt ggtggctttc tatactccgg gtacagtttt
    15001 atctgccttt gtatcgagcg cccttctctt ctttgtcatg gcggcagttg gtatttttac
    15061 caagaaagac ttgagtggga ttggtcgagc aatgatggca gctctcattg gtttactgat
    15121 tgctatggta gtcaatattt tcttggctag tggcttcttt gattatatga ttagcgtagc
    15181 catggttttg gttttttcag ggttgattgc ttgggataac caaaagattc gcctcgccta
    15241 tgaacaatca caaggtcgtg tagcgacagg ctgggtggtg tcaatggctc tcagcatcta
    15301 tcttgatttt atcaatcttt tcctaagtat tctacgtatc tttggtcgca atgactaaaa
    15361 ggtctatgaa atcagtgttt tgagtgacac tgattttttt aaattttctc ttttcttttc
    15421 ttggaaatag gtgtataata cgtttattaa atttttgagg agttgtctat gaagaaaagt
    15481 tttatccatc aacaagaaga aatttccttt gtcaaaaata cttttaccca gtatttgaaa
    15541 gataagctag aagttgtcga agttcaaggt cctatcttga gtaaggtcgg tgacggaatg
    15601 caggacaacc tgtctggtgt ggaaaatcca gtatcggtca aggttctcca aatccctgat
    15661 gctacttatg aagtggtgca ctcacttgct aaatggaaac gccacacctt ggctcgtttt
    15721 ggctttggtg aaggagaggg tctctttgtc cacatgaaag cccttcgtcc agatgaggat
    15781 tccttggatg caacccactc tgtttatgtt gaccagtggg actgggagaa ggttatccca
    15841 aatggtaagc gtaacatcgt ttatctaaaa gaaacagttg agaagattta taaggctatt
    15901 cgcctgactg agctagctgt tgaagcccgc tatgacatcg agtctatctt gccaaaacaa
    15961 atcaccttta tccatacaga agaattggta gaacgctacc cagacttgac acctaaagaa
    16021 cgtgaaaatg cgatttgtaa agaatttgga gccgtctttt tgattggtat cggtggcgag
    16081 ttgccagatg gtaaaccgca cgatggacgt gcaccagact atgatgactg gacaagcgag
    16141 tctgagaatg gctacaaggg tctaaatggt gatattcttg tctggaatga gtctttaggt
    16201 ggagcctttg agttgtcttc tatgggaatt cgtgtagatg aagaaactct tagacgtcag
    16261 gtggaaatta caggtgatga agaccgcttg gaattggaat ggcacaagtc tttgttgaat
    16321 ggcctattcc cattgacaat cggtggagga attggacaat ctcgtatggc catgttccta
    16381 cttcgcaaga gacacatcgg agaagtgcaa acaagtgttt ggcctcaaga agtccgcgat
    16441 acttacgaaa atattttgta gagaatcgaa ccgcaaggtt cggttttctt tctctttttg
    16501 tctataattt ggtataataa acagtatgaa aatcgtatca ggaatctatg ggggacgtcc
    16561 cctcaagaca ctagaaggca agacaacaag acctacttcg gataaggtta ggggagccat
    16621 ttttaacatg attggtccct actttgaagg gggacgagtc ttggaccttt atgcaggtag
    16681 tggtggttta tctatcgaag cagtatcgcg tggcatgtcc agtgctgttt tggtggagcg
    16741 agaccgtaag gctcagacca tcgtggctga aaatatccag atgaccaagg aagttggaaa
    16801 atttcaactc ctcaagatgg atgcagagag ggcattggaa caggtatctg gggaatttga
    16861 cctcgttttc ttagaccctc cctatgccaa ggaacaaatc gtagcagata ttgaaaaaat
    16921 ggctaagaga gagctttttt ctgaagatgt tatggttgtg tgcgagacgg ataaggccgt
    16981 tgaacttcca gaagagattg cctgtctggg tatctggaag gaaaagattt atggaattag
    17041 taaggtgaca gtctatgtca gataagattg gcttattcac aggctcattt gatccgatga
    17101 caaatgggca tctggatatc attgaacggg cgagcagact ttttgataag ctttatgtgg
    17161 gtattttttt taatccccac aaacaaggat ttctccctct tgaaaatcgt aaacgggggc
    17221 tagaaaaggc tttgggacat ttggaaaatg ttgaagtcgt ggcttctcat gatgaattgg
    17281 tggtcgatgt tgcaaaaaga ctgggggcta ctttcctagt acgggggttg aggaatgcgg
    17341 cggatttgca atatgaagct agttttgatt actacaatca tcagctgtct tctgatatag
    17401 agactattta tttacatagc cggcctgaac atctctatat cagttcatca ggtgttcgag
    17461 agcttttgaa gtttggtcag gatattgcct gccatgttcc cgagagtatt ttggaggaaa
    17521 taagaaatga aaaaaaagat tagatggccc ttatacgtca ttgcggcctt gattgtgact
    17581 ttcttggcat ttgtagtgcc cttgccttat tatatagagg ttccaggtgg ttcggaagat
    17641 attcgccaag tccttaaagt aaatgacaca gaagataagg aagctggtgc ctatcaattc
    17701 gttacggttg gtgttcaaca tgccacttta gctcatatga tttatgcttg gttgacacct
    17761 tttacagata ttcgtagtgc tcaggagact acaggtggtt cttccgatgt tgaatttatg
    17821 cgaatcaatc aattctacat gcaaacatcg caaaatatgg ccaagtatca aggactaaaa
    17881 acagctggta aggatatcga actcaagtac tttggagttt atgttttgaa tgtgacggat
    17941 aattcaacct ttaaagagat tctcaatatc tctgatacag tcacagcagt caatgatcag
    18001 acctttgata gttccaaaga cttgattgat tacgtcagtt ctcaaaaatt aggggattcc
    18061 gtcaaggtca cctatgaaga ggatgggcaa accaagtctg cagaaggaaa aatcatcacc
    18121 ttggaaaatg gcaaaaatgg aattggaatc ggcttgattg accgtacaga ggtaatcagc
    18181 aatgtcccaa ttagcttttc aacagctggt attggcggtc caagtgctgg tctcatgttt
    18241 agtctagcta tctatactca aatagctcac ccagatcttc gtaatggtcg tattgttgcc
    18301 ggtacaggta ccattgaccg cgatggtaat gtgggagaca ttggaggtat tgataagaag
    18361 gttgtagctt cggctagggc aggtgctgct attttctttg ctcctgataa ccctgttagc
    18421 gaagaagaac aaaaggcgca tccggacgcg aaaaacaact accaaacagc cctagaagcg
    18481 gctaaaacaa tcaagacgga tatgaaaatc gtgcccgtta aaaccctaca agatgcaatt
    18541 gattacttga aaaacaatcc ctaatttttg agtgaagttt ccaaactagc gcacaaacag
    18601 agaagatggt ataatagtca aatggttcaa ttattattca ctctaagtag tcatatgttc
    18661 tttatttatg tgagttttta ccttttaaag gatcttgtta gatgggagaa ggttttaaaa
    18721 gtgacagatg ataatacaag aaaagttcgt ttattagtag ccttttttag cattgtcata
    18781 ggctacatcc tgagttcttt ctttattagc ctgtatcatt tgtggcaaga agcgcttaga
    18841 ggattattat gaaatcaaga gtaaaggaaa cgagtatgga taaaattgtg gttcaaggtg
    18901 gcgataatcg tctggtagga agcgtgacga tcgagggagc aaaaaatgca gtcttaccct
    18961 tgttggcagc gactattcta gcaagtgaag gaaagaccgt cttgcagaat gttccgattt
    19021 tgtcggatgt ctttattatg aatcaggtag ttggtggttt gaatgccaag gttgactttg
    19081 atgaggaagc tcatcttgtc aaggtggatg ctactggcga catcactgag gaagcccctt
    19141 acaagtatgt cagcaagatg cgcgcctcca tcgttgtatt agggccaatc cttgcccgtg
    19201 tgggtcatgc caaggtatcc atgccaggtg gttgtacgat tggtagccgt cctattgatc
    19261 ttcatttgaa aggtctggaa gctatggggg ttaagattag tcagacagct ggttacatcg
    19321 aagccaaggc agaacgcttg catggcgctc atatctatat ggactttcca agtgttggtg
    19381 caacgcagaa cttgatgatg gcagcgactc tggctgatgg ggtgacagtg attgagaatg
    19441 ctgcgcgtga gcctgagatt gttgacttag ccattctcct taatgaaatg ggagccaagg
    19501 tcaaaggtgc tggtacagag actataacca ttactggtgt tgagaaactt catggtacga
    19561 ctcacaatgt agtccaagac cgtatcgaag caggaacctt tatggtagct gctgccatga
    19621 ctggtggtga tgtcttgatt cgagacgctg tctgggagca caaccgtccc ttgattgcca
    19681 agttacttga aatgggtgtt gaagtaattg aagaagacga aggaattcgt gttcgttctc
    19741 aactagaaaa tctaaaagct gttcatgtga aaaccttgcc ccacccagga tttccaacag
    19801 atatgcaggc tcaatttaca gccttgatga cagttgcaaa aggcgaatca accatggtgg
    19861 agacagtttt cgaaaatcgt ttccaacacc tagaagagat gcgccgcatg ggcttgcatt
    19921 ctgagattat ccgtgataca gctcgtattg ttggtggaca gcctttgcag ggagcagaag
    19981 ttctttcaac tgaccttcgt gccagtgcag ccttgatttt gacaggtttg gtagcacagg
    20041 gagaaactgt ggtcggtaaa ttggttcact tggatagagg ttactacggt ttccatgaga
    20101 agttggcgca gctaggtgct aagattcagc ggattgaggc aagtgatgaa gatgaataag
    20161 aaatcaagct acgtagtcaa gcgtttactt ttagtcatca tagtactgat tttaggtact
    20221 ctggctctag gaatcggttt aatggtaggt tatggaatct tgggcaaggg tcaagatcca
    20281 tgggctatcc tgtctccagc aaaatggcag gaattgattc ataaatttac aggaaattag
    20341 gctggagaac cagccttttt ctaaagataa ggagaaatat gaacaaaaaa acaagacaga
    20401 cactaatcgg actgctagtg ttattgcttt tgtctacagg gagctattat atcaagcaga
    20461 tgccgtcggc acctaatagt cccaaaacca atcttagtca gaaaaaacaa gcgtctgaag
    20521 ctcctagtca agcattggca gagagtgtct taacagacgc agtcaagagt caaataaagg
    20581 ggagtctgga gtggaatggc tcaggtgctt ttatcgtcaa tggtaataaa acaaatctag
    20641 atgccaaggt ttcaagtaag ccctacgctg acaataaaac aaagacagtg ggcaaggaaa
    20701 ctgttccaac cgtagctaat gccctcttgt ctaaggccac tcgtcagtac aagaatcgta
    20761 aagaaactgg gaatggttca acttcttgga ctcctccagg ttggcatcag gtcaagaatc
    20821 taaagggctc ttatacccat gcagtcgata gaggtcattt gttaggctat gccttaatcg
    20881 gtggtttgga tggttttgat gcctcaacaa gcaatcctaa aaacattgct gttcagacag
    20941 cctgggcaaa tcaggcacaa gccgagtatt cgactggtca aaactactat gaaagcaagg
    21001 tgcgtaaagc cttggaccaa aacaagcgtg tccgttaccg tgtaaccctt tactacgctt
    21061 caaacgagga tttagttccc tcagcttcac agattgaagc caagtcttcg gatggagaat
    21121 tggaattcaa tgttctagtt cccaatgttc aaaagggact tcaactggat taccgaactg
    21181 gagaagtaac tgtaactcag taaaagataa gcctaaactc ctatgtcact tatggatgta
    21241 ggagttcttt ttactagttt aagcagggct agaacaggta ctaagaaaaa atagtaactt
    21301 ctaaaactaa cttccagttt cccacaacct agcttttagt atgagaaaaa cgcgtgaaat
    21361 cagtggaaat ccgtcttaga ctaacttcca gttttgggag agagatggaa gttacgctaa
    21421 gaaaaaaata atatgtattg gattaaattt tgtgatataa taaaggataa gatactattt
    21481 taggagaaga actatggaag acccaagtag tcagaatttg ttgctacaat ttgttttatt
    21541 gtttatcttg acggtattaa atgccttttt ctcagccaca gaaatggcca tggtttctct
    21601 taatcgtgcc cgagttgaac aaaaggcgga agaaggagac agacgctaca ttcgcctgct
    21661 gaaggtacta gaaaatccta accacttttt atcaaccatt caggtaggaa tcaccctgat
    21721 tacaatctta tcaggggcga gtttaactga tactctagga cgtgtgattg cctcttggct
    21781 tgggaatggc gaaacagcac aagccgtggc aacttttcta tccttggcat ttttgaccta
    21841 tatttccatc gtttttgggg aattatatcc taagagaatc gctcttaatc taaaggatgc
    21901 cttggcaatt cgtacagcgc cggttatcat tgggatcgga aaactagtca gtccttttgt
    21961 ttggctttta gctgcttcga ccaatttctt gagccatttg actcctatgt cattcgatga
    22021 tgcggatgaa aagatgaccc gtgatgaaat tgcctatatg ctgacaaata gtgaagaaac
    22081 attggatgct gatgagattg agatgctaca aggtgtcttt tcgctcgatg aactgatggc
    22141 acgagaggtt atggttcctc gaacggatgc ctttatggtg gatattcagg atgatagtca
    22201 agccattatc caaagtattt taaaacaaaa ttattctcgt atcccggttt atgatgggga
    22261 taaggacaat gtaattggaa tcattcacac caagagtctc cttaaggcag gctttgtgga
    22321 cggttttgac aatattgttt ggaagagaat tttacaagat ccactttttg tacctgaaac
    22381 tatttttgtg gatgacttgc taaaagaact gcgaaatacc caaagacaaa tggcaatctt
    22441 gctggatgaa tacggtggta tggctggttt agtgactttg gaagacctct tagaggaaat
    22501 tgttggtgaa attgatgatg aaacggataa agcagcaata gatgttcatc agattggcga
    22561 ggatacctac attgttcaag gaactatgac cctcaatgat ttcaataatt actttgatgt
    22621 tgaactggaa agtgatgacg ttgataccat cgctggttat tatttgacag gagtgggaac
    22681 cattccaacg actgagaaac tcagctatga attggtcagc caaaacaaac agtttatctt
    22741 aaccaatgat aaagtgaaga atggacgtgt taccaaggta aaagtccaaa tcacagaagt
    22801 cgaaatagaa gaagaaacag aataaactag aggcttttgt caccaggcgt gacgaagctt
    22861 ttttcaattg atttcaaaag ggaattatgc catggaaaga actctgccac aaactcgctc
    22921 caaaagtttt taaagttata cgtatttatt cgagggaaaa taaaaaaagc ccttcaaatt
    22981 gggctttctg ttcatttgaa acctgataaa tcaagttata gaaggcggta gacggatttg
    23041 aaccgacgat caagcttttg cagagccgtg ccttaccact tggctatacc gccttaacgt
    23101 ttattattat accttgaaaa tgattttcag tcaatagttt tttcagtttt ttgtaccata
    23161 gcgtgattcg aatgtgatgc caagttttct atgggaaaaa aattaccaaa aacctactga
    23221 atccccttga aaaatctagc taaatagggt ataatatggg taatcatttg tcgtaggttt
    23281 tgtctgaaat attgtccaga caaggctcac agcagttaaa tcttctgaaa aagtcagatt
    23341 tagctgctct ttttgtgctt tttttcaaga ttttgagcat ttgtaacaga ggcttaaaga
    23401 ttctgaaaat tcgtcaagag gacacggtga taaggggttt acaaccatat ggcgattaga
    23461 aaagcctgat tgacaaggct tggaacttat ttacaaagga gaatcatctt ggcaggacat
    23521 gacgttcaat acgggaaaca tcgtacccgt cgtagttttt caagaatcaa agaagttctt
    23581 gacttaccaa atttgattga aattcaaact gactcattca aagctttcct agaccacggt
    23641 cttaaggaag tgtttgaaga tgtattgcca atttcaaact tcacagacac aatggagttg
    23701 gaatttgttg gatatgaaat caaggaacca aaatacacgc tagaagaagc tcgtattcac
    23761 gatgctagct actcagcacc aatttttgta accttccgct tgatcaataa agaaacaggc
    23821 gaaatcaaga cccaagaagt tttctttggt gatttcccaa tcatgacaga aatgggtact
    23881 ttcatcatca atggtggtga acgtattatc gtttctcagt tggtccgctc accaggtgtt
    23941 tactttaacg acaaagtaga caaaaatggt aaggtgggct atggttcaac tgttatccct
    24001 aaccgtggag cttggttgga acttgaaagc gactcaaaag atatcaccta cactcgtatc
    24061 gaccgtactc gtaagattcc atttacaacc ttggttcgtg ctcttggttt ctcaggtgat
    24121 gatgaaatct ttgatatttt tggtgacagc gaattggttc gcaacactgt tgaaaaagat
    24181 atccacaaga atccaatgga ctctcgtaca gacgaagcct tgaaagaaat ttacgaacgc
    24241 cttcgtccag gtgagcctaa gacggctgaa agctcacgta gcttgcttgt ggctcgcttc
    24301 tttgacccac gtcgctatga cttggcagca gttggtcgtt acaaaatcaa taaaaaactc
    24361 aatgttaaaa cacgtttgct caaccaaacc attgcagagc cattggtaga ccctgaaact
    24421 ggagaaatct tggtagaagc tggtacgatt atgactcgta gcgtgattga aagcattgaa
    24481 agccatttgg atggcgactt gaacaagatt gtctacatcc caaacgatgc agccgttgtg
    24541 actgagcctg ttgttcttca aaaattcaag gttgttgctc caactgatcc agatcgcgtc
    24601 gtaacgatca ttggtaatgc taacccagat gacaaggttc gtacggtgac tcctgcagat
    24661 atccttgctg agatgagcta cttcctcaac ttggctgaag gacttggccg tgtagatgat
    24721 atcgaccacc ttggaaatcg tcgtatccgt gcggttggtg aattgcttgc caaccaagta
    24781 cgtttgggac tttctcgtat ggaacgtaat gtccgtgaac gtatgtctgt tcaggacaat
    24841 gaagtcttga caccacaaca aattatcaat atccgtcctg taacagctgc agttaaagaa
    24901 ttctttggtt catcacagtt gtcgcagttc atggaccaac acaacccgct ttctgagttg
    24961 tctcacaaac gccgtttgtc agccttagga cctggtggtt tgactcgtga ccgtgctgga
    25021 tatgaagtac gtgacgtgca ctacactcac tatggtcgta tgtgtccaat cgagacacct
    25081 gaaggaccta acatcggttt gatcaataac ttgtcatctt acggacactt gaacaaatat
    25141 ggttttgttc aaacaccata ccgtaaggtt gaccgtgaaa caggtgttgt cacgaacgaa
    25201 attgtttggt tgacagctga tgaagaagat gaatatactg tagctcaggc taactctcgt
    25261 ctgaatgaag atggaacctt tgctgagaag attgtcatgg gacgtcacca aggggtcaac
    25321 caagagtatc cagctaatat tgttgactac atggacgttt caccaaaaca ggtagttgcc
    25381 gttgcgacag catgtattcc tttcttggaa aacgatgact ccaaccgtgc cctcatggga
    25441 gccaatatgc aacgtcaggc tgtgccattg attaatcctc aggcacctta cgttggtact
    25501 ggtatggaat accaagcagc ccacgattct ggtgcggctg tgattgctca gtatgatggt
    25561 aaagttactt acgcagatgc tgacaaggta gaagttcgtc gtgaagatgg ttcattggat
    25621 gtttaccaca tccaaaaatt ccgtcgttca aactcaggta ctgcttacaa ccaacgcact
    25681 ctcgtaaaag ttggtgatgt cgttgaaaaa ggcgatttca tcgctgacgg gccttctatg
    25741 gaaaatggag aaatggcgct tggacaaaac ccaatcgttg cctacatgac ttgggaaggt
    25801 tacaacttcg aggatgccgt tatcatgagc gaacgcttgg tgaaggacga tgtctacaca
    25861 tctgttcacc ttgaagaata cgaatcagaa acgcgcgata caaagcttgg gcctgaagaa
    25921 atcactcgcg aaattccaaa cgttggtgaa gatgccctca aagaccttga cgaaatgggg
    25981 attatccgta ttggtgctga ggttaaagaa ggtgatattc ttgtaggtaa agtaacacct
    26041 aagggtgaga aagacctttc agctgaagaa cgtctcttgc acgctatctt tggagacaag
    26101 tctcgtgaag tgcgtgatac ttctcttcgt gtaccacacg gtgccgatgg tgtcgttcgt
    26161 gatgttaaga tctttacacg tgtaaatgga gatgagttgc aatcaggtgt taacatgttg
    26221 gttcgtgttt acatcgctca aaaacgtaag attaaggtcg gagataaaat ggccggacgt
    26281 cacggaaaca aaggggttgt ctctcgtatc gttcctgtag aagacatgcc ttaccttcca
    26341 gacggaactc cagtcgacat catgttgaac ccacttgggg tgccatcacg tatgaatatc
    26401 ggtcaggtta tggagcttca ccttggtatg gcagctcgta ctcttggtat tcacattgcg
    26461 acaccagtct ttgatggagc aagttctgaa gatctttggt caactgttaa agaagcaggt
    26521 atggatagcg atgccaagac aatcctttac gatggacgta caggtgaacc atttgataac
    26581 cgtgtttctg ttggagtcat gtacatgatc aaactccacc acatggttga cgataaattg
    26641 cacgcgcgtt cagtcggacc ttactcaact gttacccaac aaccactcgg aggtaaagct
    26701 cagtttggtg gacaacgttt cggtgagatg gaggtttggg ctcttgaagc ctacggtgcg
    26761 tcaaatgtcc ttcaagaaat cttgacttac aagtcggacg atatcaacgg acgtttgaaa
    26821 gcctatgaag ctattacaaa aggcaaacca attccaaaac caggtgttcc agaatccttc
    26881 cgagttcttg tcaaagaatt gcaatctctt ggtcttgaca tgcgtgtcct agacgaagat
    26941 gaccaagaag tggaacttcg cgacttggat gaaggaatgg acgaagatgt catccacgta
    27001 gatgaccttg aaaaagcccg cgaaaaagca gcccaagagg ctaaagcagc ctttgaagct
    27061 gaagaagctg agaaagcaac aaaagcggaa gcaacagaag aagctgctga acaagaataa
    27121 gcagttcact tagaatagaa agggaagaaa tagtggttga tgtaaatcgt tttaaaagta
    27181 tgcaaatcac cctagcttct ccaagtaaag tccgttcatg gtcttatgga gaagtcaaaa
    27241 aacctgaaac aatcaattat cgtaccttga aaccagaacg tgaaggactc tttgatgaag
    27301 tgatctttgg tcctacaaaa gactgggaat gtgcttgtgg taagtacaaa cgcattcgtt
    27361 acataggaat tgtttgtgac cgctgtgggg ttgaagtaac gcgtacgaaa gttcgtcgtg
    27421 agcgtatggg acatatcgaa ttgaaagctc ctgtatctca catctggtac ttcaagggga
    27481 ttccaagccg tatgggcttg acccttgata tgagccctcg tgccctcgag gaagttatct
    27541 actttgcggc ttatgtggtg attgatccta aggatacacc acttgagcac aagtctatca
    27601 tgacagagcg cgaataccga gagcgcttgc gtgaatatgg ttatggttca tttgttgcca
    27661 agatgggtgc ggaagccatc caagaccttt tgaagcaagt agatcttgaa aaagaaattg
    27721 ctgaactcaa agaagaattg aaaacagcta ctggacaaaa acgtgtcaaa gccatccgtc
    27781 gtttggatgt tttggatgcc ttttacaagt ctggaaacaa acctgaatgg atgattctta
    27841 acatccttcc ggttatccca ccagatcttc gtccaatgtt gcagttggat ggtggccgtt
    27901 ttgcctcatc tgacttgaat gacctttacc gccgtgttat caaccgtaac aaccgtttgg
    27961 ctcgtttgct tgagttaaat gcaccaggta tcatcgttca aaatgagaag cgtatgcttc
    28021 aagaagcagt tgacgctttg attgacaatg gtcgtcgtgg tcgtccaatc acaggaccag
    28081 gtagccgtcc attgaaatca ttgagccaca tgcttaaagg taaacaggga cgcttccgtc
    28141 aaaacttgct cggtaaacgt gttgacttct caggacgttc cgttatcgcc gttggtccaa
    28201 ctcttaagat gtaccaatgt ggtgtgccgc gtgaaatggc gattgaactc tttaaaccat
    28261 ttgtcatgcg tgaaatcgtt gcccgtgata tcgtgcaaaa cgtcaaagca gctaaacgct
    28321 tggtggaacg cggagatgag cgtatctggg atatccttga agaagtgatt aaagaacacc
    28381 cagtgctttt gaaccgcgca ccgacccttc accgtttggg tatccaagcc ttcgagccag
    28441 tcttgattga tggtaaggct cttcgcttgc acccacttgt ctgtgaagcc tacaatgctg
    28501 actttgacgg ggaccaaatg gccatccacg taccactttc agaagaagca caagcagaag
    28561 ctcgtatcct catgctagct gctgagcaca tcttgaaccc gaaagatggg aaaccggtag
    28621 ttactccatc tcaggacatg gttttgggta actactactt gaccatggaa gaagctggtc
    28681 gcgaaggtga aggaatggtc ttcaaagacc gtgacgaagc ggttatggct taccgcaatg
    28741 gttatgttca cctccactca cgtgttggta tcgcaacaga cagcctcaac aagccttgga
    28801 cagaagagca aagacataag gtcttgctta caacagttgg taaaattctc ttcaacgata
    28861 tcatgccaga ggggctacca tacttgcaag aaccaaacaa tgccaacttg acagaaggtg
    28921 ttccagctaa atacttcttg ccacttggtg gagatatcaa ggaagctatc agcaatcttg
    28981 agctcaaccc tccattcaag aagaaaaacc ttggaaatat catcgctgaa atcttcaaac
    29041 gtttccgtac gacagaaact tctgccctac ttgaccgcat gaagaacctc ggttaccacc
    29101 actcaactct tgcaggattg acagtgggta ttgccgatat cccagtcgtt gatgacaagg
    29161 ctgaaatcat tgaagaatca cacaaacgtg tcgaacaaat caccaaacaa ttccgtcgtg
    29221 gtatgatcac agacgacgag cgttacaatg ctgttacagc tgaatggcgt gctgcccgtg
    29281 aaaaacttga gaaacgcttg attgctaacc aagatcctaa gaacccaatc gttatgatga
    29341 tggactctgg agcccgtggt aacatctcaa acttctcaca gcttgccggt atgcgtggtc
    29401 tgatggctgc tccgaatgga cgtatcatgg aattgccaat cctttcaaac ttccgcgaag
    29461 gtttgtcagt attggaaatg ttcttctcaa ctcacggtgc tcgtaaaggt atgaccgata
    29521 cggcccttaa gacagccgac tcaggttact tgactcgtcg tttggtcgac gttgcccaag
    29581 acgttatcat ccgtgaggac gactgtggaa ctgaccgtgg tctcttgatc cgttctatcg
    29641 cagaaggaaa agagatgatc gagtctctcg aagagcgtct caacggtcgt tacactaaga
    29701 aaactgttaa acatccagaa actggtgcag tgattattgg tccaaatgaa ttgattacag
    29761 aagacaaggc gcgtgaaatt gtcaatgctg gtgtggaaga agtgactatc cgttctgtat
    29821 ttacatgtaa cactcgtcac ggtgtctgcc gtcactgtta cggtatcaac ttggcgactg
    29881 gtgatgcggt tgaagttggt gaagcagttg gtacaatcgc tgcccaatct atcggggaac
    29941 ctggtacaca gcttacaatg cgtaccttcc acacaggtgg ggttgcctca aataccgata
    30001 tcactcaggg tcttcctcgt gtccaagaaa tctttgaagc ccgcaatcct aaaggggaag
    30061 cggttatcac agaggttaaa ggacaagtta ctgctatcga agaagatgca tcaactcgta
    30121 ccaagaaagt ctttgttaag ggtgaaactg gcgaaggtga atatgtcgtt ccatttacag
    30181 ctcgtatgcg tgtcgaagtt gggggccaag tagcgcgtgg tgctgctctg acagaaggtt
    30241 ctatccaacc aaaacgtctc cttgcagttc gtgatgtctt gtcagttgaa acgtaccttc
    30301 tcggtgaagt acaaaaagtt taccgtagcc aaggggtaga aatcggtgac aaacacatcg
    30361 aggtaatggt tcgtcaaatg atccgtaaag tccgtgtcat ggatccaggt gatacagatc
    30421 ttctcatggg taccctcatg gatatcaatg actttacaga tgccaacaaa gatgtcctta
    30481 tcgcaggtgg agttccagcg acaggtcgcc cagtccttat gggaattacc aaagcctcac
    30541 ttgaaacaaa cagtttcttg tcagcggctt ccttccagga aacaactcgt gtccttactg
    30601 acgcagctat ccgtggtaag aaagaccatc tccttggact taaagaaaat gttatcatcg
    30661 gtaagatcat cccagctggt actggtatgg cccgttaccg taaccttgaa ccacatgctg
    30721 tcaacgaaga agaatacctt aatcctccag tagaggaaga aggaaatgaa gaaacaacag
    30781 aagtagttgt ggatactgcc gttgaaactg tggaagaaac agtagaataa gagagaatga
    30841 gagaaccctc gggttctctc attctctctt ctgaaaactt tctccatttt tccacgaaat
    30901 gtggtaaaat aaaagaaaga agctgacaaa ggagacgggt atggaacaaa cattctttat
    30961 catcaaacca gatggtgtaa aaagagggct agtgggtgaa gtgttaaagc gcatcgaaca
    31021 acgtggattt acaatcgaaa aattggagtt tcgttcacag gtttcagaag agttgattga
    31081 ccagcactat caggacttgg ttggtcagag tttttaccca ccgattcgtg aattcatgac
    31141 ttcaggtcca gttcttgtgg gtgtcatttc tggtcccaaa gtaatcgaaa cttggcggac
    31201 catgatgggt gcaactcgtc cagaagaagc tttaccaggc actattcgag gtgattttgc
    31261 aaaagctgct ggagaaaatg agattatcca aaatgttgta catggttcag attcagaaga
    31321 gtcagctaag cgagaaattg ctctttggtt ttaagagtgg attggctcaa tcaattggtt
    31381 aaaagctcat ttgaatagaa agtatagtca attagtttaa gacatgacgc atgatatcaa
    31441 actttttagt ttttgatatg gtgcgttttt tgattaagtg actattagtt tgtctcgctc
    31501 cgttaggtgc gagacaaaaa aaacacccgc tatgcgggtg cgcgtcgaag gttataccaa
    31561 aaaaactcca aacgcgatac aataaaggtg ttcaatccaa ttgtaaagcg aaaggagaaa
    31621 aatatggcac aaaaggttct tttatcctgt tcttgtttca attgactata tttctatact
    31681 ttttcttcct ttctctgtga aaatcgcaag tttttcccac cttttctcaa tactgaaaca
    31741 acatctttcc agaacttcaa taatttcttt ttgtgttaaa tcattattta actaaatctt
    31801 tggaggcaat acgttttcaa agcgactttt ttaccatagg ttttgagcct tcaacacaga
    31861 ttactaagcc aaaaatgctc taaatttttg aaattctaac aaattccact caactttatc
    31921 atcatctttg actttcacaa tatcttcctc aaaataacat ggaataaaga tagttttctt
    31981 ttgaaacggt tgtaggagga aaatctctcc tacactatta ttgattctga gaggaaattg
    32041 caaaatatca tctaccaaat tttcaattat taagattttt tgcagtttat ctgaattaga
    32101 atcgcaaggc ttctcaataa tataattgtc gaattttttc attctaactt cctctccact
    32161 atttcctcct ttatcaagaa aaagttacct caatattaat aaagtacaat tctaaattaa
    32221 ctccaattat tgattcatga taatatttca gtaactcatc gtgcaattaa aaacaaggat
    32281 ttttatttca gatgatccat cgtgaacact tcatcagcca tctcccaaat tgccggatta
    32341 tgtgttgcga taatgattag cctattatca tctcgaagag atagcaaaat ctccataatc
    32401 aactgagagg ttgctgggtc tattgaagct gttggctcat ctgccagaat aaagggtgga
    32461 ttctttaaga taatttttgc caaggcaact cgttgcgatt ctccgcccga taactcaaag
    32521 atgcgcttat ctaggtcaag ataagccagg ccgacctgtt ctaaagcctg cttctgcctc
    32581 aaccgctgtt ccgaccgact caacttttga ccaatgagac ctagcttaag gttttcttca
    32641 atactttggt tttcaattaa gccaaagttc tggaagaggt agcccaattc gtgacggaaa
    32701 aaatcactcg atttataatt ggccaagtct ttacctcggt aaaaaatcgt cccatcataa
    32761 ggttctaatt tcccaatcat gttcatcaag gttgtttttc cgctaccact tgaaccaatt
    32821 aaggcataga cttttccagc ctcaaatgtc attgaaagat tcgaaaataa ctctcgttct
    32881 ccaaaagatt tactcacctg tttaagttca atcatattaa cctcccttca aaacaagaat
    32941 ggacatcttg ttttctttct gcatttggac atgtaactgt aaaagagata gaccagtaaa
    33001 gagtaacaag actaagaaag caactcctat ctctacctga agaaatacac tcgcaacaaa
    33061 tcccagtaaa aacacaccca gttgagcaaa gagataagtg cgatggattt ctaagaacct
    33121 gagacctgca atgcgtttga taaaaatggc acgtctaaat tcttcaaagt agagcctatt
    33181 catagtgtta aacaacaaga ttgaagttgc aatcccaagc acagctcctg ctagcattac
    33241 ccaacgttcc ctctggatat tatccaataa tgtgatgtag ttgtggtaac ctgtttgcat
    33301 ttctgagacc caattttcaa tgccttgtct ctggataagc tcctgggcat cagacaattg
    33361 attaaagaga acgtagttct gtactgcgtc tatccaaaac aaaatggact gtggaccagt
    33421 tgattggggt gttataacaa tgatgattgg atctttcaaa aactgctggt aagaaatagg
    33481 ggtcgtatta tacacaaaac gatcctgacc tgattctaaa taacctaccg tagcagtcat
    33541 ttgctgtcgt tcatcttgac tagacatgcg actggttaag tcgtcttcaa aaacagattt
    33601 ataatgttct tcctctgaac ggaggtgttc aggcagcaat aagacaaact cccctacatt
    33661 aaggtgattc atcttttgct taatagttgt atctacagga atgttttgac gctccaagta
    33721 atgcggtgtg acgataagga cattgccatt cgggctgtaa tcgtgccatt ctgtagaggt
    33781 tgtcaagttt ttggaggaag ccatgccatt ttgcaaagta cggtcaatta actggtgtct
    33841 agataagaaa gccttttgtt ctgaaacagc cagatccatc aattgatacc aagttctgag
    33901 tttcctttga gcttgttcat caaaaccagg actcgttcct tcacggctga ttgatagggt
    33961 aatcagttgt ctttcctgac tccaaacctc ttgtccaatc cgatgctgtt gccaggcttg
    34021 ggaatacttt aagctactcc ctattcccag cgttacaata ataatcgcta acagttgacc
    34081 gattaaaatc aaactaatga ttcctctgac aggcacttgc ccttttataa tctgcatcag
    34141 gtgtatcttt tttatcccaa ctgcgaagag ttgagcgaaa aacagggaga tcgacaacaa
    34201 gatgagatta taactgagca agcctgctcc tatggtcatt agggattgcg ttggaagcga
    34261 cagaattttt tgcataagaa tggcgagcac gccagctaaa ctgaaaccaa cagctatccc
    34321 tttcaaatcc tcaccaactg gtctaaggaa aatggaccac cgtttctctc ctgaaatgag
    34381 gcgaatgccc gatgaccgta attggctaat ttgactaatt aaagtcagtg ctacaaaggt
    34441 taagatgaaa atcaatagac tgattaactg aaatccgtta ctaaagatag ccattaacgt
    34501 agatagttta gatggaattg tcaggtgcat gttagtcaag ccaagttgac ttagctcttc
    34561 ccttagcaag tgaatatcta ggtgtccatc gaatacaaaa tagtttgttt caacactact
    34621 actttgagca tcttctagat ttttttcctg aagcccatca ggcaactttc cgtccccaaa
    34681 ggtcgtataa gaaaaaactg tagtaccttc tgagttagga tcttggtgtt gaatcgcaat
    34741 aaagctatct gtttcttctg ctagtttttc caagcgtgta gcaacatgct gaaaagttgt
    34801 ttcaggggaa gaatcacgta caacaacgtt ggggtaataa tgggagacat atgtatcagt
    34861 ccaaatggta aatacccaaa caaagaacaa actagcaagc aggttggata tgagtataaa
    34921 taattttttc atagtgcatt ccttattcta aaacagaggg cagaaatact cctgccctct
    34981 ataaactaac aagcttacta aactattgat aaaagaatct tcttcaatta ttctttacca
    35041 tcccgcatca aatgtaacta attttttaat tccacgtttt tttctcatgc aatgaaaccc
    35101 ctttcttttt ccttgttttt attttaacag atatatttta tttagccaag catttttatg
    35161 taatttttga ctttttttag tttattacca taaa
//
LOCUS       DAVKJJ010000019        34408 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000019 DAVKJJ010000000
VERSION     DAVKJJ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34408
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.019"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(<1..666)
                     /locus_tag="VW144_000907"
     CDS             complement(<1..666)
                     /locus_tag="VW144_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9600374.1"
                     /translation="MCYTCCSTTNTRKAQRCKTIILQKANKGKSNREIASLLGKAPQT
                     IHTEIKRRTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHN
                     QKFSPEMMVMAKGVNVGISTIYYWIHHGKLGLSKQDLLYPRKGKALKKQASTNFKPAG
                     QSIEQRPEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSAEVV
                     NQAL"
     gene            complement(1040..1438)
                     /locus_tag="VW144_000908"
     CDS             complement(1040..1438)
                     /locus_tag="VW144_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9600375.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            complement(1811..2293)
                     /gene="greA"
                     /locus_tag="VW144_000909"
     CDS             complement(1811..2293)
                     /gene="greA"
                     /locus_tag="VW144_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU9600376.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(2364..4019)
                     /gene="mltG"
                     /locus_tag="VW144_000910"
     CDS             complement(2364..4019)
                     /gene="mltG"
                     /locus_tag="VW144_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU9600377.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSTEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQTVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTINELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTDIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(4100..4594)
                     /locus_tag="VW144_000911"
     CDS             complement(4100..4594)
                     /locus_tag="VW144_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9600378.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAITPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(4591..5055)
                     /locus_tag="VW144_000912"
     CDS             complement(4591..5055)
                     /locus_tag="VW144_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9600379.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESAHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(5065..6399)
                     /locus_tag="VW144_000913"
     CDS             complement(5065..6399)
                     /locus_tag="VW144_000913"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU9600380.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGSDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(6410..7027)
                     /locus_tag="VW144_000914"
     CDS             complement(6410..7027)
                     /locus_tag="VW144_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9600381.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPITEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSTPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(7075..10173)
                     /locus_tag="VW144_000915"
     CDS             complement(7075..10173)
                     /locus_tag="VW144_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9600382.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLIFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIHLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPRQATQVQADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            complement(10266..11432)
                     /locus_tag="VW144_000916"
     CDS             complement(10266..11432)
                     /locus_tag="VW144_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9600383.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSAKLNNRRLITNSLVEKDSLFEIDFDQLEKDLVEEE
                     VKLYVLCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKC"
     gene            complement(11440..12534)
                     /locus_tag="VW144_000917"
     CDS             complement(11440..12534)
                     /locus_tag="VW144_000917"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9600384.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSGFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQAIEG"
     gene            complement(12692..14374)
                     /locus_tag="VW144_000918"
                     /pseudo
     CDS             complement(12692..14374)
                     /locus_tag="VW144_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768988.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(14546..16504)
                     /locus_tag="VW144_000919"
     CDS             complement(14546..16504)
                     /locus_tag="VW144_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9600385.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            complement(16708..18330)
                     /locus_tag="VW144_000920"
     CDS             complement(16708..18330)
                     /locus_tag="VW144_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9600386.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            18436..19881
                     /locus_tag="VW144_000921"
     CDS             18436..19881
                     /locus_tag="VW144_000921"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEU9600387.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMEKSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISNYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            complement(19924..20121)
                     /locus_tag="VW144_000922"
     CDS             complement(19924..20121)
                     /locus_tag="VW144_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9600388.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTKE"
     gene            complement(20222..20830)
                     /locus_tag="VW144_000923"
     CDS             complement(20222..20830)
                     /locus_tag="VW144_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEU9600389.1"
                     /translation="MFGELIRYLDQYEDVILREIKAQFPDVAVDKLMEEYIKAGLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKYQL
                     MQKCRDIFVDSLVVLGYIVQNEDGKYELAIDFDKERLTFYLA"
     gene            complement(20855..21367)
                     /locus_tag="VW144_000924"
     CDS             complement(20855..21367)
                     /locus_tag="VW144_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU9600390.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            complement(21453..22388)
                     /locus_tag="VW144_000925"
     CDS             complement(21453..22388)
                     /locus_tag="VW144_000925"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEU9600391.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            complement(22459..22758)
                     /locus_tag="VW144_000926"
     CDS             complement(22459..22758)
                     /locus_tag="VW144_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU9600392.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDVRRRLAIHNSGRGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            complement(22748..23497)
                     /locus_tag="VW144_000927"
     CDS             complement(22748..23497)
                     /locus_tag="VW144_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEU9600393.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            complement(23551..23910)
                     /locus_tag="VW144_000928"
     CDS             complement(23551..23910)
                     /locus_tag="VW144_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU9600394.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            complement(23912..25312)
                     /locus_tag="VW144_000929"
     CDS             complement(23912..25312)
                     /locus_tag="VW144_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEU9600395.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILIGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            complement(25607..25846)
                     /locus_tag="VW144_000930"
     CDS             complement(25607..25846)
                     /locus_tag="VW144_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU9600396.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            complement(25878..26348)
                     /gene="ssbA"
                     /locus_tag="VW144_000931"
     CDS             complement(25878..26348)
                     /gene="ssbA"
                     /locus_tag="VW144_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEU9600397.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            complement(26360..26650)
                     /locus_tag="VW144_000932"
     CDS             complement(26360..26650)
                     /locus_tag="VW144_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU9600398.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            complement(26803..28149)
                     /gene="asnS"
                     /locus_tag="VW144_000933"
     CDS             complement(26803..28149)
                     /gene="asnS"
                     /locus_tag="VW144_000933"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU9600399.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            complement(28165..28530)
                     /locus_tag="VW144_000934"
     CDS             complement(28165..28530)
                     /locus_tag="VW144_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9600400.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGVPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            complement(28523..29710)
                     /locus_tag="VW144_000935"
     CDS             complement(28523..29710)
                     /locus_tag="VW144_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9600401.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAVVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            complement(29707..30195)
                     /locus_tag="VW144_000936"
     CDS             complement(29707..30195)
                     /locus_tag="VW144_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEU9600402.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEKADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGISQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            30516..31064
                     /locus_tag="VW144_000937"
     CDS             30516..31064
                     /locus_tag="VW144_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9600403.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
     gene            complement(31342..32070)
                     /gene="yaaA"
                     /locus_tag="VW144_000938"
     CDS             complement(31342..32070)
                     /gene="yaaA"
                     /locus_tag="VW144_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEU9600404.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(32149..32313)
                     /locus_tag="VW144_000939"
     CDS             complement(32149..32313)
                     /locus_tag="VW144_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEU9600405.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            complement(32480..34075)
                     /locus_tag="VW144_000940"
     CDS             complement(32480..34075)
                     /locus_tag="VW144_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600406.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPRSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRVYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
     gene            complement(34087..34326)
                     /locus_tag="VW144_000941"
                     /pseudo
     CDS             complement(34087..34326)
                     /locus_tag="VW144_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
BASE COUNT         9918 a         7712 c         6410 g        10368 t
ORIGIN      
        1 tagagcctga ttgaccacct cagcactttt atttggaatc aatcggatga tctgatgtct
       61 actctttcga tccgtcaaga caagcaagca gtagtttttc gctctcgtaa gtagaaccgt
      121 atcaatctca taatgcccat tctccaagcg aagattgata gcttcaggcc gctgttcgat
      181 ggattgacca gcaggtttaa agttggtgct agcctgtttc ttaagcgctt ttccttttct
      241 agggtaaagc aaatcctgct tgcttaaccc caattttcca tgatgaatcc aatagtaaat
      301 ggttgaaatt cccacgttaa cccctttagc cataaccatc atttcaggcg aaaatttttg
      361 gttatgatag tggagaatct tttcctttag ttccttggtc aagcttgatt tcttgaccga
      421 gcgcttgcga ttgttttcat aagactgttg agcatagtcg gcagaataaa cctctttgaa
      481 gcgccctttt ccaagacatt gtcggactgt cctacgcttg atttcagtgt ggatagtttg
      541 aggagctttt ccaagtagag aggcaatttc tctatttgat ttccctttgt tcgccttttg
      601 tagtataatg gttttgcatc tctgtgcctt tcttgtgttt gtggttgaac aacaagtata
      661 acacaaaggt gttttcttat gcctacaaga gctttcatta tttccatttt cttttggatt
      721 tcactctatt ctgaaaaact tgtgtatatt tactgaagct agcaagtctt acctgtaaat
      781 ttaatgaaag caacacaaaa tccgagaggg gaatctcgga ttaatgtcaa tttaaaaaga
      841 tagagagttt ttagtttaaa taaattgttt taaaatatca acaacatcac ttcttttctt
      901 aacctgataa gccttgattc ctaattttgg ggctacgatt atattgtcct caatatcgtc
      961 tagaaagaca caatttctag gttataactg gtatttatcg atagttactc atatatatca
     1021 gtccacctcc atactcacct tacagattct tttgataata atatctttgc ctaatgtaga
     1081 gatagtcttg caaagaaaaa acttccttgt agccatgttt ctgataaaag tccggtgcct
     1141 ggaactggta agtattgaca aaggcaaaac aacaatttcg attcttagct tcactttctg
     1201 cctgttgcaa tagttttgaa ccgattcctt gccctcgcag ttcctctttt acaaacaaat
     1261 actcgatttc tagccaattt ccaaaagtct ctgctatcaa acccgcaagg agattgccct
     1321 tttcatcttc gacataaaga ttaagtggct cactttcagc ctcttctctt tttgaacggt
     1381 tataaacacg aatcagattc cctatttctt gcgatttatg tgattcctta ttttccaatc
     1441 taaagtataa tgaaatgaaa taaaacatgc gcaaatcgat taaggaattt aatctaattt
     1501 ctaacaatgt cttagaaatc aaagtgtact atttcaactt caatgcacta tacatctaat
     1561 actcaataaa aatcaaagag caaactagga aactaggcgc aggctgctca aaacaccgtt
     1621 ttgaggttgt agatgaaact gacgaagtca gctcaaaaca tggttttgag gttgtagatg
     1681 aaactgacaa agtcagtaac catatatacg gcaaggcgac gctgacgtgg tttgaagaga
     1741 ttttcgaaga atagcaaaat ggaaaaagga gtgagtgaag cacatcgcct ccccactcct
     1801 ttttctgttt ttaggctgtt ttttcaacct tcaagatttt tacatcatag ctaccaacag
     1861 gcgtttcaat ggttgctgtg tcacctgttt tcttgccaat caaggcctgc ccaattgggc
     1921 tttcatttga aaccttacct gcaaaggcat ccgcaccagc tgaacctacg ataatataaa
     1981 cttcttcttc gtcctcacca atttcttgga tggtgactgt tttaccaatc gctacttcgt
     2041 cctgggcaac tgcgtcgcta ttgacgattt cagcatagcg gatttttgtt tctaagctag
     2101 agatttgtcc ttcgacaaag gcttgttcat ccttagctgc ttcgtactca ctgttttctg
     2161 aaagatcacc gtatgaacgg gcaatcttaa tgcgttctac cacttctggt cgacgaacca
     2221 atttcaattc ttctaattct ttttcaagtt tctccttttc ctcaagggtc ataggatatg
     2281 ttttttctgc catttttctc aactttcttc tgataatatt ttctaaagaa aattatgtga
     2341 agtatcacat aattttagtt tgtttagttt aatttgctgt tgacatgttc agcgacattg
     2401 cggtcgtggt cttcttgatt gttagcatag tagaccttgc cttctgtgac atctgctaca
     2461 aagtagaggt tatcgctctt agtttgattg atgcttgact caatcgcatc cagacttgga
     2521 ctatcgactg gaccaggcat gagacctaca tttttataaa cattataagg tgaatcaatg
     2581 tcggtatcaa tcgcaacatc ctcagctaga ctgatatttt gccccagttt tccttgggca
     2641 tacaagattg caatattact ttgaagtggc atatcacgat ttaaacgatt gtagaataca
     2701 cctgcaatga gcttacgatc ttctgtcttg gcaccttctt tttcgaccaa ggaagcaatg
     2761 gtcaacaact cattgatagt caagttttta gatttgatag tactatagta aggagatagg
     2821 ttcttatcca tagcagctaa catctcatca atcaagctct caatagttgt gctttccttg
     2881 atagagtatg tagctgggaa aaggtatcct tccaaacgat aacgcgcacc gctgtctttt
     2941 acaggcaaac tttccagtaa agtaggatat ttcgctactg cttgactgat aaacgtctca
     3001 tcttgaactt tagccaagaa agcctccgct gtcaaagact ctttgaagtc accttgcaat
     3061 tgacccacag tttgagcaat ctgatccaag gtataacctt ctggaattgt caaagtcgca
     3121 agtacaggtt cttgcggttc atctgttcca cctttttgca actctttgag taagtcttct
     3181 gtactcatac tcttttgcaa attgtagtaa cctgctttca agtcggtata atttttatac
     3241 ttggcataaa aactaaaaat cagaccatgc tttaccaaac cagctttttc aagcgtcgta
     3301 ccgatttctt gaacgtttga accttctgga attccaaccg tcacatattt cttagaatta
     3361 gcatcgatag gtaacaaaga atctagcacg tactggtaac caaagtaacc acctgctgag
     3421 agcaaggcaa ggaatacaag gaatgaaatc acaaaagctt tcacatgtga cttcttctct
     3481 ttcttaggct taacgggttt tgctccttct ctacggctac gacgtgatgt atctctgatg
     3541 atatccactg tttctgtagc aggcgtaggt gtttctggac ctgcttgttc tgcttttttc
     3601 tctgccgtcg tataggaaac agctaccctt gttggggttt cattgtattc tctttcaagt
     3661 ttcttaggtc taacaggacc tggacctggt cttgatccac tttcttccgc tggagaagaa
     3721 ggtacatctt gacttggatg acttggaaca ccaggagttt ctctttgaat ctcatctgct
     3781 ggagaagctg gtacaccttg acttgggtga gtaggcacgg taggagcttt tctcataatc
     3841 tcctctaccg ttgacaagga atcagccatg agttcttcag ttgaaggttc atttgcagga
     3901 gtgcgaacta ctgcctcatc ttctttcaga acttcatcat agccttttac tttttctaaa
     3961 tctctcagaa tctgctcttt aaagcttaat ttctcttctt ctcttgactt ttcactcaaa
     4021 agtttttcct ccttgttgat aatccataat attatatctt aaaagtccaa aaaaagcaat
     4081 ctatgatact tttcctaact tattttttca tttcccagac catatcatac catgtttccc
     4141 ctgcaaaggt tgactgggac tggccttcgt tgacaaatcc atgtttttca tagtaggcga
     4201 tgagatagtc atgacaggtt agattaatgc catctctctc atcttcaaga gcaacttctt
     4261 tcaaggctgt cagcaatttc tggcccagtc cgagtccctg tgcctccttg gcaatagaca
     4321 gacaggtcac agagatataa ccaccaggct catgactata gtcttttatt tcttctgtaa
     4381 aagactggtc ttgcagatgg cggtgcaggc caactggtcc ttcgatataa cccatgattc
     4441 ttccttcttt ttcagcaacc agaaaagagg tctgaatttc tctcaaatgt gcttcaaaga
     4501 cagaaggagt aatggcttct tcgaccgaaa aattttcaaa ttcaagttca acaatccgat
     4561 ccaaatcttc taatcttgct tgtctgattt tcattgttcc tccagataaa agggattaaa
     4621 ccaaatcata ctatagccct ggctagttgc atagagcaaa gttgcttctt catcaacaaa
     4681 accgttcatt tcaaaatagg aaagcagctc atcaggactc tccaaacgaa tccctttgta
     4741 atccagctca actgccacct ctttcaaggc tgcaagaaga agtgttccca ggccctgtct
     4801 ctgatgggca gactcgatga ctaaagaatg tacttttaga cattgcggat tgtctgactg
     4861 gggacttgat aaaatatagc ctaaaagttg attttcatcc ctagctagaa gaaaggtatc
     4921 cgcacactta cggatacttt cttctaaaat atgggaaagt tgctgctttt cagctggaaa
     4981 agacgaggtc tgaagtgccc ctatctcagg caaatcagac ttgcttgcct gaatgatctt
     5041 aattggaatt tccatgggaa catcctattg aacattgctt gtcaagttag acaagagacg
     5101 ctcaaatgag tattcatagg tttggatgtc tcctgctccc ataaagacgt aaacagcatt
     5161 gtcatggtct aggagcggag aaacattttc aacagtaatc acttggtgtt ttttgttgat
     5221 tttgttggct aggtcttcta ccttaacgtc accatgatct acttcacgag ccgagccata
     5281 aatttgcgct agataaacag catctgcttg gtttaaagca tgggcaaagt cgtccaacaa
     5341 ggcaatggtt cttgtaaagg tatgcggttg aaagactgct acaatttcct tgcttgggta
     5401 tttctgacga gccgcatcca aggtcgcaat aatttctgtt ggatgatggg caaagtcatc
     5461 aataatcact gtatcattga caattttctc agtgaaacga cgtttaacac cggcaaatgt
     5521 tttcaagtgc tcacgcacca agttcaaatc aaatcctgct gtgtaaagaa gaccaataac
     5581 ggctgtcgca ttcatgatat tgtgacgacc aaaggttgga atgtggaatt gccccaagtt
     5641 ttgtccacgg aaatgaacgg tgaaggttga accagttgtt gaacgaagaa gatcactagc
     5701 tacaaagtca ctgccttcag cttcaaaacc ataataataa attggtgcat cagacgtaat
     5761 cttacgcaat tcagcatctt caccatagac aaaaagaccc ttggtaattt gtttggcata
     5821 gtcgttaaag gcattgaaaa catcctcgag acttgtgaaa taatctggat ggtcaaagtc
     5881 aatgttggtg ataatagagt attctgggtg gtaaggcatg aagtgacgct catattcgtc
     5941 agattcaaag acaaaatatt tggcattagc cgaaccacga cctgtcccat ctccaatcaa
     6001 gaagctggta tctgtaatgt gagacaagac atgagacaac atacctgtcg ttgaagtttt
     6061 tccatgtgct cctgctactc ccatgctaac aaagtcacgc ataaagctac ctagaaactc
     6121 atggtaacgt ttgtagctga taccattttg gtccgcatag gcaatttcga cgttgttatc
     6181 tggacgaaag gcatttccag cgataatttc catatcaccg tctagattct tttcatcaaa
     6241 aggaagaatg gtaattcctg cctgctcaag accgcgttgg gtaaagtagt acttttcaac
     6301 atctgatccc tgaaccttgt gccccatctg gtgcaacatc aaggccaagg cactcatccc
     6361 tgatccctta attccgataa aatgatatgt ctttgacatg ttttctcccc tattctgtca
     6421 ttctggtcag attcaactct tgggcaaccc gacgttcttg ttctgtttgt ttactttttt
     6481 tattgtagat ttggctcttc tttagaaaat cataattgtt tttctttgga gcaggtgttg
     6541 acacttcttc attcttggta gggatagaat gaacttcttc cgccaagata taatgagact
     6601 gggtcaattt ttggctatat ttgacaaatt caccaggatt ttccttttgg aaaggagttg
     6661 tcggttgatt gccctgtcta actagactgg gctgagaatg acgtctcgca aggctgaaat
     6721 cctgagttag gtagttagca gagcgtttct ttttcaagtc cgcacgcgct tcttcacgcg
     6781 ccacctccgc atagctcttt ccttcttttt taacccctaa aggagccttt ttaggttttt
     6841 cgacttgctt ttcaatcggt tttactggtt tttcttcagt aataggagcc cattctaaat
     6901 aatttttatc tcgatactca cccttgatat tactgatcag atcagactca tcatagagat
     6961 tcatgactgg catttcagtc aacatgacct cgtcatctga caccaatgga aatcgttctt
     7021 gtttcatttt ctatttcctt tcaacacttc attatagcgt attgtcttga tttttcaagt
     7081 gctggcttca gaaattccca aaatttctct aatttctgct agggtcagac taccacgtga
     7141 ctctgtgcca tccaatactt gtgacaccag atgtttcttt tgttcttgga gttcctgaat
     7201 tttttcttca atggttccct tggtcaccaa gcgatagacc tcaaccgttt cttcctgacc
     7261 catccgatgg gcacggccaa tggcttgcgc ttccaccgca ggattccacc aaaggtcaac
     7321 caagatcact gtatctgcac ctgtcaggtt cagaccgacc ccaccagcct tgagggaaat
     7381 cagaaaggca tctctttctc cttggttaaa ggccttggtc atgtcttgtc tttccttggc
     7441 tggggttgaa cccgtaattt taaaggatgt caggcccaag tctggcagtt cttgttcaat
     7501 tttttccaac attcccttga actgagagaa aatcaagaca cggtgtccgc cgtctgccac
     7561 ctgtaccagt aggtctcgga gactatctag tttgccgctg gctccctgat aatcttccat
     7621 aaacagggca ggagtgtcac atatttgacg caagcgcatc aaaccagata aaatttccac
     7681 acgacttcgc tgaaattcct gttctgacac ttgagccaga tggtctcgca tctgttgtaa
     7741 ctgggcaagg taaatagcct tttgctggtc ttccagttca tttttataaa ccacctcaat
     7801 caagtctggc aattcagtca gaacttcttc tttcttgcgt cgcatcacga aaggcttgat
     7861 aaactgagcc actcgctctg ctggcaattt cataaattct ttcttgcttg gcaaaagtcc
     7921 gggcatgacg atttggaaaa tagaccacaa ctcacccaga tggttttcaa tcggagttcc
     7981 tgacaaggca aagaccgacg gcaccacaaa ttgtctcaag gtctgggcaa tcttggtctg
     8041 ggcatttttc atgacctgag cctcatctaa gaaaaggaag tcaaaggcca tcccctgata
     8101 aaactcactg tcctgacgga aggtggcata gctagtcaca tagatttgat ggctctcggc
     8161 aagaatctcc tcacgacttg ctttcaaacc atgaacaaca gccacatcca actgtggggc
     8221 aaatttctga aactcatctg cccagttgta aatcaaacct gacggagcga gaatcaaaac
     8281 ccgactttct tttgtcactt gactagtcaa aaaagcaatg gtctgaaggg ttttcccaag
     8341 tcccatatca tcagccaaaa tcccaccaaa accataatga tggagcatct gcaaccagcc
     8401 aattcccttt tcctgataat ctcgcaagtc agcctgaacc tgagttgcct gtcgaggaaa
     8461 gtcctctgga tgtgtcaaat cctgggccag attctggaat tcttgtgaaa aagaaacacg
     8521 gtctcgccct tcaaagagat gagctaaact gtaggccaag gatttccgag cctgcaaggt
     8581 cccatctttt aattcaaatt gccccagttc ctgtagattt tggcgaattt tcttggtttc
     8641 ttcatcgaaa aagtaaactt gattagacga atcaatataa aaatcctgat tggcaaccaa
     8701 ggcctgcatg gcttggtcga tttcctcctg ggcaatatct tgaaaatcaa actggatttc
     8761 caagagacct cccttggagg caatctgcac ctgaggactc gctaggctat aaagctcttc
     8821 tagtttatct gataggtcaa catgcccgag tttttcaaag actggaatga tatcatgaaa
     8881 aaaatgatag acagactccg cttttaaggc ctgacgccaa gattgaaaat cggcttcaaa
     8941 gcccgcagcc aaacagactt ggaaaattct ttcttctaag tccgcgtcac ttgaaaatgg
     9001 taattcttct agctcttgtc ggctagatac ctgtctattt ccataatcaa actgaatttc
     9061 taaatgaatc cgattatctt cttccctgtc aaagtaaaaa gagggcgcaa aagttttgat
     9121 ttgtagacgt tctggagctg aaacggtgcc catctggata aaaagagtca gacaggaggc
     9181 caatttgtct cgatcactgc tatcaaattg caggtatttc tttccttgtt gacccacagg
     9241 taacgcttta atttccttga gaagacgcat ctgctggtct gttaaaaaat aaacctgacc
     9301 tttatggaaa agtactgctc cctgataaaa gacattgacc ctaggactct cactgatttc
     9361 catttcaaaa tagtccgaat attctgttac tgtaaaggca aatagattgg catcagcatg
     9421 catatcctga aaaagcaggg tttggtagct atccacttga tggtcaaatt gaaaatgggg
     9481 caaggccatc agtaaattca caccctgctc aaaaaaggtc agagggaaaa agaggtgccg
     9541 accttggttt tggaaaaaga ggtctggaac cagcccttcc tccgttagtc cgtgcaagaa
     9601 agtcaaaagt tcttggctgg catcatcaaa ggcttcccaa gaaagagact cctcataaat
     9661 cttgccaatc atatacgact ttctctgctc gacaatcctt aaaaaaagtg gaatatcccg
     9721 aatgacatag tatttttggc tattgatttg gccgattctc agagtccaca agatatgatt
     9781 ggttcctgct tccacctgac ccacagctga taactcatag gcgcattctg attttggaga
     9841 taaaattcga tccaaaaact tgccacccaa ggtcaccttg gtttcaacgg cctctttttc
     9901 ttcatgacct tcttccagac tccacaagat ttcctgacca cgctcatcat ttttcagaaa
     9961 atgctctagc gctgccaaat gcacacagta gcccctcttt tgaaaaaaat cacaggcaca
    10021 aaaaatcaaa tcatcctcta aactatagcg cagttcttct tctgcaacgc gagcgtagag
    10081 ccgattgttc ttttccttga tgatatcaac cttaccagtt tcataaaggg caacaccttc
    10141 gatacgaatt ttccccggaa tcaatttagc catattttca cctttacctt atctttttat
    10201 tataccatat tttcgcctat gaaaatagcc ttctaggaag acttttctcc tagaaggctg
    10261 gatttttaac atttggcaaa agtagccaca atccgctgac agacttcttg caacagagat
    10321 ttgggcatag ctatattgat gcgggcatgg agacttcctt cctctccaaa atccaaacca
    10381 cggttgagga taaccttggc ttcatttctc aacaactctt gcaatgtttc atcagtcagg
    10441 tcataagctg aaaagtcaag ccaaatcaag taggtacctt gcggtttcat gaccttgatt
    10501 ttagtctctt ttccaaatag atccaccaca taattgatgt ggtcttcaaa gacttgcttg
    10561 agttcctcta gccaatcttt accgtatcga taggcagctt ctgtcgccaa ataacccaag
    10621 cctgaaattt catgctgatt attggccaac aggcgtttct ggaaagccag tctcaactta
    10681 ggattttcaa tgactgcata ggaatttttt gttccagcaa tattaaatgt tttagtggca
    10741 ctgctcaaga cgatagcaaa atttttgaag gcaggattga tggtattgaa agactggtgt
    10801 ttgtgaccaa agagggtcaa atcttggtga atctcatccg aaactaacaa aacaccgtgt
    10861 ttttggcaga gttggccaat cttctccaac acttcttttt cccaaacacg tccaccagga
    10921 ttgtgagggt tgcaaagaac atagagttta acctcctctt ccaccaaatc cttttcaagt
    10981 tggtcaaagt caatctcaaa cagactatcc ttttccacta aggaattagt aatcaatcta
    11041 cgattattca acttggcact gcgagcaaag ggtgggtaga caggcgtgtt aattaaaacc
    11101 gcctcgcctt cttttgtaaa ggtttgaata gctgttgaga tggctggtac cacaccctcg
    11161 ataaagacaa gagcctcttt gtcaaagttg taaccgtatt gtgtagcttc ccacttttga
    11221 acttccttaa ttaagtcttc actggcatag gtataaccat aaaccagttg gtctgcgtaa
    11281 gtttgcacgg cttggcggat ttcaggcaag accacaaagt ccatatccgc tatccaagct
    11341 ggtagaactt cactatccgt ttctgtttct ttccatttat aggtatggtg ccctaaacgg
    11401 ttgggcaggc ttgtaaaatc atattttccc atctttgtct tatccttcta tggcttggcg
    11461 caaatctgca atcaaatctc tagcatcctc aatcccaata gacaaacgca agaggtcatc
    11521 tgtcaaacca taagaatggc gtacctctgc tggaatatca gcatgagttt gagtcgttgg
    11581 ataagtaata agactttcca ctccacccaa actttccgca aaagagaaga ccttgagact
    11641 gttcaaaata tgaggaatgc gtgtttcatc ggctacttta aaggaaatca tgcctccacg
    11701 accagtgtag agaacttcct taactgctgg agaatccttc aaaaaggcaa ccacttcttg
    11761 ggcgttagct gttgagcgct ccatacgaag agacaaggtc ttgagaccac gaagcaactg
    11821 gtagctgtca aatggagaca agactgcccc tgttgtatta agattgtaaa aaagcttctc
    11881 gtatagttct aaactattgg tcacaaccac tccagccaag acatcattgt ggcctgctag
    11941 atacttggtt gctgaatgga gaacgatatc tgctccatct tcaatcggac gttggtagat
    12001 agggctatag aaggtattgt ccaccaccac tttggcaccc ttagcatgag ccaattttgc
    12061 tagtttttcg atatcaaatt ccaacatcaa gggattggtt ggggtttcga tatagagaac
    12121 atccacatcc ttttctaact cagcaatcaa ctcttcttct gtattggcat aggtaaaatg
    12181 gaaacgacct tcctgctcca cttggttaaa ccagcgaaaa gaaccaccgt aaagatcacg
    12241 cactgccaag accttacttc ctactggaaa gccgctaaag gccagtacaa tagctgacat
    12301 ccctgagcta gtcgctaggg catagtctgc tgactcaata gccgccaaga cttcctcagc
    12361 cttactacga gttggatttt tagtgcgcgt atagtcaaac ccagtagatc gaccaaactc
    12421 tggatgctga taggtcgttg aaaaatgaag cggtgtcacc aatgcacctg tcgcttcatc
    12481 tgacttaata cccgcctgag ccaaaattgt gttaatgtgt aattccttgc tcatacaata
    12541 cctccaaatc tatagtaact attgtaccac ttattttcat caactcattt tcttcttttc
    12601 aagagctagt tatagtttca aactatataa aaagggagtt tttcctgctc cctttaatag
    12661 actataaaat ggtgaatctc aaaagacacc ttcactctat catttgctcc tgcacaaaac
    12721 gagcataacg ctcatgattt tccagtagtt ccttatgagt tcctgagcca gtgattttcc
    12781 ccccctctaa gaagaaaata caatccacat cttttaccgt tgacaaacga tgcgctataa
    12841 tcacaaccgt cttctccttt aatgcagaat agaggatact gataatcgca tactcagaat
    12901 ccgcatcaag attagcagtg gattcatcaa atataagaat ttcagcatct tttaagtagg
    12961 ttctagctat ttgaagtctt tggctttgcc cccctgacaa gaatcgtccg cgttcaccaa
    13021 cttcagtatc tagtccctct ttcatggagc gaatctcatc acctagtgat actaagtcta
    13081 gcactttcat caattcatca tcagttacta agcgattcaa accgagacaa agattgtcac
    13141 gaatactgcc agataagact gcattatttt gtgaaaccca agcgatttta cttctccatt
    13201 cttttaagtt aaaatcatat atacttgatt gctccattag aatatatcct gaaagcggtt
    13261 tataaaaccg ctctaacaaa cgcacaatcg ttgattttcc tgatccagat ggtccaacaa
    13321 aagcaatttt ttgccccttg aaaattgaac aagtaatatc ctttaagaca ggtcgatttt
    13381 catcataacc aaaatagaca tggttaaaat tcaacccttg tcctgatacc gattttcctc
    13441 actcaaattt ttctttagga actgcaagca agttctccag tgcaactgaa gatcccttgc
    13501 tcctagaata aatagttaca aaattagcta tattactaat aggattaagt aattgaaaga
    13561 ggtaaatcaa aaacgaaacc aaggttccca cagatatata tcctgcgctg acccgataac
    13621 ccccataggt tagcatcaca actatagtcg caaagataaa taagagagca aacggggtct
    13681 caaaagaagt aaccctatct gatttcagtg aattgttttg taccctttca atacaattat
    13741 ccaaaacatc ctgtacactt ttctctgctt ggttagtctt aattaattca tgttcttgaa
    13801 tcttttcagt caattgccct gttaaaaatc cgatttcctc ctgtaaacga cgactatact
    13861 tttcactgat attggaaagg ggcaagataa taaacatcat acaaggaaga gtgatgaata
    13921 aaagtagaga aagattccaa tcaagactaa ataagactac aatggaacca agtaccataa
    13981 ctaaactcag aataatattt gggaaagtcg taattaaaaa ctcacgaatg acactcgtgt
    14041 cattgacaat ggcagaagtc aactccccac tttggctctt atcaaagaag gatttctctg
    14101 cataaatcaa cccctctatc acttttttcc tgatttttgc tatctttttt tcacccgatt
    14161 gactaaacag atagtaacca atagaagaaa acaaggcttg accaataaaa atcaaaaacg
    14221 attgaaatac tttggaacct atattttcaa tagaactccc atctattaaa ccctttaaga
    14281 taaggggaag caacaaagca agtagactag acagaacaag taagaaactc cccataatca
    14341 ccttagtatc tactcttaat aattttaatt tcataaatac tccttataat atttcaacgg
    14401 ataaagtcga gaataactca atttgaggat aaaatctaat aaatcttcct ataacaaaac
    14461 gcataacatc taggatttta tatacctgat attatgcgtt tttaagcaca aagacttctt
    14521 acacaaactt atctacaatt agattttatt tgacatgttt tgccaattct tcttgggctt
    14581 ttttattgga ttcttctttt tctttcaacc atttttctct ggcttttgca tattcgtctg
    14641 ttgtgacaat cttatcttgt actttgaggt atttatatga ttcaacccct tttgtaccgg
    14701 ttaaaccata ggcagcagca aatggtacgg ttcttctcaa tgatggtgtt cccccacgcg
    14761 aaacacttgg aagaactaaa gaactatcaa tcaaccaagc ttgaatatca gcatatttct
    14821 cataacgttt ggctggatct tgctctttat tagcttcttc caacatttga gtatagacat
    14881 ccagtccaac tgccttagcc ttgtcattgg cttctccagg ctctagtcca agattttgta
    14941 gaactcctcc actattagta ttaaaaatat cgagataggt tgacgggtct tgataatcag
    15001 gtccccaacc gccatgatat aaatcatagt ctttctgagc agctgtttga gcaaagtagc
    15061 ctgaactgtc aaactcatct gatgttaatt gctgaatgtc aatcactaca ttatcagaac
    15121 ctaaaacaga ttcaattgat tgtttgatag aactaactcc ttgtatgcct actttatctg
    15181 ttacttccac agtcttatcc aagtggattg ggaattgaac accctttgct tcgagttctt
    15241 tcttagcttc cgcaaactta gccttggctt tctcaggatt gtagtaaggg tcttgaccat
    15301 ccgcaaagtt gataccttgc cattccttac catagttgac catcttagag gctacaactt
    15361 caccaaagtc ttttcccttg atactgacaa agtttggagg aaccactagg ttacgcaaaa
    15421 tctttgttgc accttctttc ccttcagact gagccccata agatgttctg tcaaaagcaa
    15481 aattgatagc ctgacggaag tttttattga gaactgcttc ctgagtcgat ttcttttcaa
    15541 tgtcacttgt tttagaagta taattgtaag acttcctatc taggttaaaa ttaaagaaat
    15601 atgaagttga attttgcata ctatagatga tattgttttt gtatttttct ttaatccctt
    15661 catagctgga gctgttagga aaaagacgag ccgtagtata agcaccagct gtaaaattac
    15721 gttccagtga ttcttggtcg ctaccatcat agtaggtcaa tttcacatcg tctacaaaga
    15781 cattcttagc atcccagtaa ttagggtttt tcttatattc aatagcagat tttgagacaa
    15841 gtgctttcat caagaaaggt ccattgtaca aaatactaga tggatccgcc ttcccaaaat
    15901 catccccttt tgatttcagg aaatctgcat taacaggaaa aagtatcgtt gcaagtgttt
    15961 ttgaattcca gtaaagttct ggtttaacca aagtatattg aaccgtttgg tcatcaagtg
    16021 ccttgacacc gacagttgaa aagtcgcttg ttttaccagt gatatagtca tccaaaccag
    16081 caacagagtc ctgcactaga tacaaggctt ctgatttttt atcagctgca tattgcaaac
    16141 ctgtcacaaa atcctgggca gttacaggcg catattcttc tccctcagaa gtaaaccact
    16201 tggcatcctt acgaagtttg taggtatagg tcaaaccgtc ctgagaaaca gtccaatcct
    16261 ctgctaatga tggaataata ttcccatatt ggtcattttc taataacccg tctaccaaat
    16321 ttgcaacaat atcggatgtt gctgcgcggt tttctgctag atagttcaag ctagatggat
    16381 cacttgaata aacatagttg taggtttttg acgccgtgct agaatttcca cacgcgctca
    16441 ataaaactcc tgtacccagg acaagacctg ccaaggttag atatttgctc ttagactttt
    16501 tcatttccgg aacctcctgc tttaaatatt tgttttatta tacaactctt ggtttgttta
    16561 gtcaattgtt ttttccaaaa aatcaaaaaa aacctcattt tctatttttg aaagataatt
    16621 ttagctattt ttacgctaaa aaaggaataa gcttcaagtt ctcctaaaag attgttcaac
    16681 ttattccttt tatttataaa tgctacctta cgaaagctta aattttgctc gcaggcgatc
    16741 tgcttgggct tttcctatta ccttatctaa taaataggta cgcagactca taaccatata
    16801 aagtccaccc cccatggcac cgacaagagc tacataaaag aagctccaca aacgtccact
    16861 tggttggaag aaaaatccta acagccactg gatggttcct attaacagaa acatgactag
    16921 ggtcagcaaa ctgattaaaa tggttcgctt caaaatcacc ttgcgcttga caccagttac
    16981 tttacaaata tcccgataca tcaagacgtt aggaatgatg agagcaatgg ttgttgaaat
    17041 caaaggacca taactgtgga agagggcgat ggtaggtagt tgcaagacta gcttggcaat
    17101 agaaccatag ataaaataga gaacggcctt gcggttgcgg aacatggcct gaagcattgg
    17161 agacaagacc atgtacaagc ctaaaataat agactgcaaa actgcaaaga caaataagcc
    17221 cagagccaaa ctatctggct taccatagaa gactgtatat agaggttctc ctaccataac
    17281 cactccaacc gttgctggta gcaagaacat aaagagtagg gtgagactgt cctgaacgag
    17341 acgagaagct gctttcaagt cccccttgac atagttttcc gtcaaaagtg gcaaaccaac
    17401 actcccaatc gaaaccccta cagaaatcaa aatcatcgtg attttattag gattggctga
    17461 gaaataagaa aacatgacaa ccaagtcctc attgctgtag ttggtaaacc agctcatact
    17521 attgataaag gtcagctgat ccaaaatctg gaagagctgg atggcagacc ctgtcaggat
    17581 aaaaggaatg gcttccttaa tggtatcaac caagagacgc ttactgttaa tcttatctcc
    17641 tgtttcaaag actcttttga gtgaaccttc ttgggcaagg aaataaatca agactgcaaa
    17701 actggctacc ataccgacaa aggcagcaaa ggttgattgg gtaacggctg ctagataatc
    17761 tcctgaaccg agcttcataa tgataaaggt tgctaggagc atccagataa cacgaatgac
    17821 ctgctcagca atttggctca tggcataggg tttgaggtta ttcatccctt ggaaaaatcc
    17881 tcggataaca ctcatagacg ggaaaatcaa gactccccaa gccaagcttt gcatgattgg
    17941 gatcaagtct ttgcccacgc cagacaagtc tgctagccaa ggagcaaaga catacaagac
    18001 taaagcaaaa accaggccta gtcctgtcat aaagcctaag aaactccgaa tcagggcaaa
    18061 gctatgctct tcttctcgca tggtattata cttggcaact tgcttggcca ccgcaactgg
    18121 aatccccgct gttgaaacca acaagaacca agcatagata ttgtaaccca tggtaaagag
    18181 accatttgcc ttagctgcat aagcccccat ccagatgtac caagggataa tgtaaacagc
    18241 cccgagtagg cgactgataa agttactagc cgttagccaa gcagtccccc gtaacatctg
    18301 ggcctgctgg tgattgtttt cgtgcgacat agattcctca ttcaattttg atagctagga
    18361 aaaactcctt atcttccatt ataaactttt ccttgttact tgtaaattta cgactttcgg
    18421 tttacaatag aaagtatgat taagattgaa accgtattag atattttaaa gaaagatggc
    18481 ctttttcgcg aaattattga ccaaggtcat taccactaca actacagcaa agttattttt
    18541 gatagcatca gctacgacag ccgaaaagta acagaagaca ctcttttttt cgcaaaaggc
    18601 gctgccttta aaaaagaata ccttctttct gctataacac aaggattagc ttggtatgta
    18661 gctgaaaagg actacgaagt cggtatccct gtcatcattg tgaacgatat aaagaaagcc
    18721 atgagtttga ttgccatgga gttctatggt aatccacagg aaaaactcaa actccttgcc
    18781 tttactggta ctaagggtaa gacaacagca gcctatttcg cctataacat cttatctcaa
    18841 gggcatagac ctgctatgtt gtcgaccatg aacacaactc ttgatggcga gactttcttt
    18901 aagtcagcgt tgacaacccc tgagagtatt gacctctttg acatgatgaa tcaggctgtg
    18961 caaaatgacc gtacccacct catcatggaa gtctccagtc aagcctatct ggtcaaacgt
    19021 gtctatggtc taacctttga tgtgggagtt ttcctaaata tcagcccaga ccatatcggc
    19081 ccgattgaac accctagctt tgaagactat ttctaccaca agcgtctctt gatggaaaag
    19141 agccgagcag tcatcattaa cagtgacatg gaccacttct cagtcttgaa agaacaggtt
    19201 gaagatcaag accatgattt ctatggtagc caatttgata accaaatcga gaattccaaa
    19261 gcctttagct tttcagctac gggtaaactc gctggagatt atgatattca actcattggc
    19321 aacttcaacc aagaaaatgc agttgctgct ggacttgctt gtctccgtct cggagcaagt
    19381 cttgaggaca tcaaaaaagg catcgctgca acccgcgttc ctggtcgtat ggaagtcctc
    19441 actcagaaaa atggagccaa ggtcttcatc gactatgccc acaatgggga tagtctgaaa
    19501 aaactcatca atgtggttga aactcatcaa accggaaaga ttgctctggt tctgggatca
    19561 acaggaaaca agggagaaag tcgtcgtaag gactttggcc tcctcctcaa tcaacaccct
    19621 gagattcaag tctttctgac tgctgatgac cctaactatg aagacccaat ggccattgca
    19681 gatgaaatta gtaactacat caatcatcct gttgaaaaga ttgcggatcg ccaagaagcc
    19741 atcaaggcgg caatggctat cacaaatcac gaattagatg cagttattat tgcgggtaag
    19801 ggagccgatt gttaccaaat catccagggc aagaaagaat cctacccagg agatacagcc
    19861 gtcgcagaaa attatttata agaatagaaa aaatcaaggg aaaatgaaac ccttgatttt
    19921 ttcctattct ttagtaaaaa cgtttttcaa accttcaacg gcaccttcta cagcaccttt
    19981 tgcgtcttct acaacgtctt ttgccttagc aactgttttt tcaacagctc cttcaagttc
    20041 tgtcttgctg tctccagtaa ctttaccaaa accttctttg acagcgcctg ttgcttgttc
    20101 caatttgttt tcaagtgaca tatgattgtc tcctttagtt tattccgata tgtgttaccg
    20161 gttacatata gtttataccg aatactaagg aaagtcaaac aatgtgctca gaaacaagaa
    20221 atcacgctaa gtagaaagtt aatctctcct tatcaaaatc gatagccaac tcatactttc
    20281 catcttcatt ttgcacaata tagcctaaga caaccaaact gtccacaaag atatcacggc
    20341 atttctgcat aagctggtat tttttgagaa atttcaacaa aaaagccgtc atatacttga
    20401 gggcatactc aggattaaca tctcctaaaa tgtcatagag ctcctgctgt ttttctgtca
    20461 aaggatactg ctgtttgacc ttgtaaaaat aattggacag ggtcatttta atgcgcgcaa
    20521 agtccgtctt ttcaactaaa atagctgcat tgatttgatt gcgcaattcc gtctcaaaac
    20581 tctgctccaa caaggcttga tagaccggac tagcttctct gacaaaaatc tcttgatcca
    20641 gttcaagact atcaagtgat tcaagcgtag gaaaattgag gtaatagcgc ttattttcac
    20701 gtagaatcaa gcctgccttt atatactctt ccatgagttt atcaactgca acatctggaa
    20761 attgagcctt aatttcccgt agaatcacat cctcatactg gtccagatag cggatcaatt
    20821 ctccaaaaat ggctgtctcg tcaaacgaga tggattaaaa atctgaatca tgaagattcc
    20881 tttctttaca ttgtaacaga ggcgatgctg ccaactgact aggattggta ccagcttggt
    20941 tcagaggaac aggcagacct agttctatgt aatactccct ccaaaaatca ctaatctcct
    21001 gctccccatc tccaaatttc ataggaatag tttcaaaaat aggtaggatt tctgctatat
    21061 actgggagca gtagaaacca gctgcatctg gatagaaaga agcattgtag ggtgctccaa
    21121 gatgtttgca agctctttcc ttcaccgact ggatatccat ttctgggtaa acatagaggt
    21181 cgtataaatg attggactca aagaagtctg ccggttcttg acagacaaca ccagcctgtc
    21241 cactagcatg ataaatcatc ccatccaaat aaatggcaac atggctatag ttacctgtgg
    21301 aagtctggat ggcctgtccc atgtctgacc catctctcac aaaaatcaaa tcgccatttt
    21361 ctaacatact tcactcctag aaaaaaggag ccgaaactcc tttcgatata aggcaaacta
    21421 gactttccga ttttgagctg acacccaaaa tcttacgcat taaagctttc agtcaattga
    21481 ggtaccactt gtttcttacg tgaaacggca ccagcaagga aggcatgatt gttttcaagt
    21541 ttgaagttga aagccgcttc gaccttgtcc atattggcac caagagccaa gatttctgag
    21601 tttgagttga cgatatctgt aatcatcaag acaaagtcag agtagccgtt tgattcgttg
    21661 gcagcttgca ttgcagcttc aatttctgct tggcgttcca aaacttcagc gatgtcaact
    21721 gtgttcactt gggcaacacg gacattattt ccgttgagtt caaaagtttt agcatcgatg
    21781 tcaatcaatt cttcagcaga tttgctagcc aagttggtac cagctttcaa cattgccaaa
    21841 ccatattctt ccaagttcac accagccaat tcagccaatt caggagcaat gattttatct
    21901 gttgggtgtg ttgttggtga tttcaaaaga agggtatctg aaatcaaacc tgaaagcatc
    21961 aaacctgcaa tctctttagg cacagctaca ccatgttctt tgaacatacg gtaaacgatt
    22021 gaagacgctg atccaactgg ctccaaacgc atgtaaagtg ggcttgcagt ttcaaagtta
    22081 gccacacggt ggtggtctac aacaccgtaa acttctactt cagcgatatc tgatacagat
    22141 tgttggaatt cattgtggtc agtcaagata acttgctctg ccccctctgc tttggcagaa
    22201 gtgataacac gtggtgcttc cacaccaaaa tagttcaaga caaaggctgt ttcttcattt
    22261 ggagttccaa gggcaacagc ttccgtatcc aaaccgtaag cttcttttgc aaggtaggca
    22321 aaagctacag atgacccgat ggcatctgag tctggatttt ggtgaccaaa tactagaatc
    22381 ttggacatga taatacctca ttttgtttat tttctttatt gtagcatttt tttcgctttt
    22441 tgacaaaggt aagaggagtc aaaggactcc ccacgattct tcaaaataac tgagtaaatt
    22501 tctatcttga ttttcagata aaaattcttc cttctgtggc ctcttcttac gcttgagaag
    22561 agcttcagcc gacatggctt cttccttact ggcaaaacct tgagcataga taagtttgac
    22621 tggcaagcgt gctcgtgtat atttggctcc cctcccacta ttgtggatag cgaggcgtct
    22681 tctcacatca gtcgtatagc ctatatagta ggatccatca cgacactcca gaacgtacat
    22741 ataagcctta tgatccataa taaatctctt cgatttcggg cgtataagag ccatcatcat
    22801 tgtggacaat caaaggaggt aagaccttaa agccacttgt tgagccatcc ttgatcgcct
    22861 caatcaaaag catattggct tccttttctc tttttggata aacaaactgc aggcgcttag
    22921 gggctagatt atgtcgtttt aacgtatcca aaatatccag aagtcgatca ggacgatgaa
    22981 ccatggccaa acgcccatta gacttgagaa tactctgagc actacgacag atttcttcca
    23041 aattagtcgt gatttcgtgt cgagccaaga gataatgttc actctcgttc agattagaat
    23101 aaggatccac cttgaaatag ggtggattac acaaaatcat atccacctta ctcccctgaa
    23161 tgtgagcagg catatttttc aaatcatcgc agatgacctg catttgctcc tctaatccat
    23221 tcaaacggac agagcgttca gccatatccg ccaaacgctc ctgaatctca acagacaata
    23281 tctgtgcttg agtacgagtg ctagcaaaaa gccccactgc tccattccca gcacagaaat
    23341 ccacaatcaa ccccttctta ggaaaacgtg gaaatcgtga taagagaaca ctatccaccg
    23401 aatagctaaa aacctctcta ttttgaatga ttttgatatc tgtcgaaaag agctggttaa
    23461 tgcgctctcc tgattttaat aattgttctt cttccatggt cctattatag caaatttata
    23521 ttaacattac aaaaaatata aaactctaaa ctacttcttc ttttttaaat ggtgcagggc
    23581 ttctccagtc cagattggta acattcgtcg aaagggagca aaaccgtagt taaagcggtc
    23641 gcttgaaaag cgtctccgtc taggaaactg gtacttttct tcctccaaag tgcggataga
    23701 aagactggct ttccccgtaa attcatctaa atccactacc tgaacttgaa cctcttcatc
    23761 gactttcaag gtttcatgaa tattttcaat aaatcctgtc cgaatttctg aaatgtgaat
    23821 cagccccgta tcacccgtct ctaactcaac aaaggcaccg tagggctgaa tccctgtaat
    23881 acgccccttt agcttatcac cgattttcat cttagtcctc gatttcaata gtttcaatta
    23941 caacatcttc aactggcttg tccatagctc ctgtctcaac agcagcaatg gcatccaaga
    24001 cagcgtaaga tgcttcatca gctaactgac caaaaaccgt gtgacggcgg tctaggtgag
    24061 gtgtcccacc ttgattggca tagatttctg caatcggttc tggccaacca ccacgagtaa
    24121 tttctttctt cgaataaggt aggtgttggt tttgcacgat aaagaactgg ctgccgttgg
    24181 tatttggacc agcatttgcc atggaaagag caccacggat attgtaaagc tcttctgaga
    24241 attcatcctc aaaagattcg ccgtagattg actcgccacc cataccagtt ccagttgggt
    24301 ctccaccttg gatcataaag tccttgataa tacggtggaa aatgacacca tcatagtagc
    24361 catcttttga aagagataca aagttagcca ctgttttagg agcatgttca gggaaaagct
    24421 tgatacgtaa gtctccgtga ttggtcttaa tagtcgcaag aggaccttct actgtttcaa
    24481 tgtctacttg tggaaaatgc aattcttttt ctaccatacc aaatacttct aaggcatcaa
    24541 aaatgccatc ttcttctaat gtttttgtaa tataatctgc tttttctttg attttatcat
    24601 gagaaattcc catggcaacg ctgattccag cataatcaaa gagttccaag tcattgagac
    24661 catctccaaa aaccatgacc ttctctggtt tcaagccaag gtgttccaca accttttcca
    24721 cccccgtcgc tttggagcct gaaatcggca caatatcaga cgaatgttga tgccaacgaa
    24781 ccatgcgaag tttgtctgag agactgtcag gcaagtgcaa gtcatctccc ttatcttcaa
    24841 aagtccacat ctgatagata tcttcttttt catggaaatc gggatctaca tctaagtcgg
    24901 gataaattgg attgatagct tcactcatca tatcggtgcg agtcgacaac ttggcatcat
    24961 gactcccaac caagccatac tcaattcctt cttgcttagc ccaagagata tactcctcaa
    25021 catctgactt ttcaatctga tgctgataaa tgacctgacc ttttttatct tcgatataag
    25081 ctccattcaa agttacaaaa aagtcaggct tgagatcacg aatctctgga acaacaccaa
    25141 aaatgccacg tccagaggcg attcctatta aaattccttt ttcacgcaac tgtttaaaaa
    25201 cagtgggaat tgtagttgga ataaaccctg tctttgaatt ccgcaatgta tcatcaatat
    25261 caaaaaagac aatcttgatc ttctttgcct tgtatcttaa tttcgcgtcc atctcactac
    25321 ctctttcaat ctaactcttt ccattatatc ataaagtagg caaatcccct attttcaaaa
    25381 agtttatcat ttttatttta atttcttgga tgagaaaaga gacatattta tgaaaaagct
    25441 ccatcgtgct tttaatgtgt tctcttgttt tcaaactcgt aaaaagggag ccactgagcc
    25501 taactcgctc tctcatttca aagcttgtga aaaaagaccc gttggggtct taattcgctt
    25561 tcttgttttc aagctcatga aaaagagacc caaccgggtc ttttctttaa tcttcgttta
    25621 cgaaaggcat caaagccatt acgcgagcgc gtttgatagc tgttgttact ttacgttggt
    25681 ttttagctga agttcctgtt acacgacgag gaaggatttt cccacgttct gaaacgaaac
    25741 ggctaagaag ctcagtatct ttgtaatcaa catattcaat tttgtttgct gcgatgtaat
    25801 caactttttt acggcgtttg aatccgccac gacgttgttg agccatgttt tttctccttt
    25861 ataagtttag ttgtccatta gaatggtaaa tcatcatctg aaatatccaa tgggtttgtt
    25921 gctccaaatg gattttcatt acgtgaaaag tctggtactg aatttgtagg tgctgaatag
    25981 tttgcagttg gtgcagagta agctccacct gtgtgaccct cacgcacact acggctttcc
    26041 aacatttgga aattctcagc cacgacctct gtcacgtaga cacgttgtcc ttgctggtta
    26101 tcgtaactac gagtctggat acgacctgtc accccgataa gtgagccttt tttagcccag
    26161 ttagcaagat tttcagcctg ttggcgccac ataacgacat tgataaaatc agcctcacgt
    26221 tcaccatttt gactcttaaa tgtacggttt actgcaagag taaaagtcgc aactgctaca
    26281 tttgatgggg tataacgcaa ctcagcgtca cgtgtcatac gccctacaag tacaacattg
    26341 ttaatcatag tttaccttct tacgcgtcaa ttttgacgat catgtgacga agaatgtcag
    26401 cattgatttt tgaaagacgg tcaaactctt taagagctgc atcgtcattt gcttcaacgt
    26461 taacgatgtg gtaaagtcct tcacggaaat cttggatttc gtatgcaaga cgacgttttt
    26521 cccaagtttt tgattcaaca acagttgcac cgttgtcagt caaaatagag tcaaaacgtg
    26581 ctaccaaagc gtttttagct tcttcttcaa tgtttggacg aatgatataa agaatttcgt
    26641 atttagccat tgatatgttc ctccttttgg tctaatgacc ccaagacttt gcaaggggta
    26701 agtgaggttc gctcacaata aactattata ctagaaaaaa tttttttacg caagtaaaaa
    26761 cactagaatt cgaaaaaacg ccacatgggc gttttcctgt tcttatggtt tgatacggtg
    26821 caacatacgt gggaatggaa tagcttcacg gatatgtttt gttcctgctg cgaaggttac
    26881 catacgttcg ataccgatac caaatcctcc gtgtggaact gtaccgtatt tacgaaggtc
    26941 aaggtagaat tcatattctg tacgatccat gccaagttca tccatcttag cgacaagggc
    27001 atcgtaatct tcctcacgca tagacccacc gataatttct ccatagcctt ctggagcaag
    27061 caagtctgca caaagcacgc gctctggatt tccaggaact ggtttcatgt agaaggcctt
    27121 gatggctgct ggatagttca tgacaaatgt tggcacacca aagtggtttg aaatccaagt
    27181 ttcgtgtggt gacccaaagt catcaccatg ctcaagatgc tcgtagtcag catcttcatc
    27241 attttcatgc tcttgcaaga ggtcaatggc ttgatcgtaa gtgatacgtt tgaatggctc
    27301 tgcaatgtag cgtttcaaga gttctgtatc acgttccaag gtttccaagg cttgaggcgc
    27361 gcggtcaaga acaccttgta gaagagcttt cacataagct tcttgcaagt caagcgactc
    27421 atcatgtgtc aagtatgagt actcagcatc catcatccag aactcagtca agtgacggcg
    27481 tgtttttgat ttttcagcac ggaaaactgg accaaagtca aagacacgac caagagccat
    27541 agcccctgct tctaggtaaa gctgacctga ttggctcaag taggctggcg ttccgaagta
    27601 gtcagtttca aagagttctg tagaatcttc tgccgcattt cctgaaagaa ttgggctgtc
    27661 aaacttcata aaaccattct tgtcaaagaa ctcataagtt gcatagataa tagcgttacg
    27721 gatttgcaac acagctactt gcttacgaga gcgtagccac aagtgacggt tatccatcaa
    27781 aaagtctgtt ccgtgttctt ttggtgtgat tgggtagtct tgagattcac cgatcacttc
    27841 gatgtctgtg atgtccaact catagccaaa tttagaacgt tcgtcctctt tgacaatacc
    27901 tgtcacataa acagacgttt cttggctcaa gcgtttgata acatcaaact tctcaagtcc
    27961 cacttcttca ccaaattttt cgacaaagtt tggtttaaaa gccacacctt gaaagaaggc
    28021 tgttccatca cgcaattgta agaaagcgat tttccctttt cctgatttgt tggcaaccca
    28081 agcgccaatc gtcacttcct gaccaacata gtcttttaca tcaataatcg ttacacgttt
    28141 tgtcattatt tttccttttc ttttttattc tttatggcaa accacctcta tattgttccc
    28201 atccaggtca atcataaaag cagcataata aatcggatgc tcacttcgat aaccaggaac
    28261 cccattgtct cgcccacctg cctctaagcc agcctcataa caagcctgaa cttcttcctt
    28321 attttctgct aaaaaagcaa aatgaacagg atcttgtgtt ccctgagtca gccaaaaatc
    28381 accaccagga tgagggctgt tcggggatag aaaactaatt agagaactag tcttaaaagc
    28441 caatttatag tccaaaggag cgagaaaact cctataaaat ccttctgaaa tttgtaaatc
    28501 ctttacctta atctcaaaat gatcaatcat tctcactacc cataaatgct ttcaagcgtt
    28561 cgactgcttc tttaagcgtg tctaggtctg tcgcatagct gaggcggaca ttttctggtg
    28621 ctccaaatcc agctcctgtt accaaggcca cttcggcttc ttctaagata gcagttgtaa
    28681 agtctgtcac atccgtgtag cctttcatct ccatggcctt tttgacattt gggaagagat
    28741 agaaggcccc ttgtggtttg accacttcaa atcctggcac ctctgcaagg aggggataga
    28801 tggtattaag acgttcctca aaggcctgac gcatgctttc tacagtatct tgctcacctg
    28861 atagagcctc aactgctgca tattgggcta ctgctgacgg attcgaagtt gtttgacctg
    28921 caatcttgga catggcagcg ataatgtctg cttctccaac ggcataacca atccgccaac
    28981 cagtcatggc ataggtttta gacacaccat tgatgaccac tgtttgcttg cgaatcgctt
    29041 ccgataggct agaaatcggt gtgaactcat gaccattata aaccaagcgg ccatagatat
    29101 catctgctag gatgagaata tcattttcta cagcccagtt tccaattgcc aagagttcct
    29161 cacgggtgta aatcatacct gtgggattag atggcgaatt cagcaccaaa accttggtct
    29221 tgtcagtgcg agctgcttct aactgctcta cggtcacctt aaagtgattg tcttccttag
    29281 cagaaacaaa gacgggaacg ccttctgcca tcttgacctg atctccatag ctaacccagt
    29341 atggggttgg gatgatgact tcatcacctg gattgaccac agccataaag aaggtataga
    29401 gagaatattt ggctcccgca gcgactgtca cttgatttga cgctacagaa tagccgtaaa
    29461 agcgctcaaa gtagctattg accaccgcct taagctctgg cagacctgag gttactgtat
    29521 aaaaagaagc acgcccatct cgaatcgatg caatggcggc atcttggata tttttgggag
    29581 tagtgaaatc tggctcaccc aaggttagag acaaaatatc tctaccctca gccttcagtg
    29641 ctttggcacg ggctccagca gccaaagtca cactttcttc catttctaaa acacggttgg
    29701 atagtttcat aggccctcct tattgaccaa tgctcctgtt tcaaaatcta ctagataaaa
    29761 atcagatcct gacttaactt cccagattgg cttgtcttga taacgaccaa aggttatctt
    29821 gtcaatctcg ccagctccct tttccttaga aaccgtttct gctttttctt gtgaaatacc
    29881 ctgatttagc tgataaacgt aaatcttatg gtcatcttta ccaatcagga cagcaagcgc
    29941 ttcttgctgt ttattacgac caagaacgct gtaataagat tccaagccat tgtataaatc
    30001 aacctgatca gccttctcta atcctgcata ctgctgagct aatttttctc cttcactttt
    30061 agctgtttga tagggtttca tgctaagaga aaccatatac agaaaggaag cactgataac
    30121 cacaaacaaa atcgtcatcc ctagaccata ctgccacagt agattatttt ttgctttgtt
    30181 ttgtcttttt ttcactcgtc tattttacca tctattaagc tttattacaa gtgaatataa
    30241 gaatactctt cgaaaatctc ttcaaaccac gtcagcttta tctgcaacct caaagctgtg
    30301 ctttgagcaa ccaattctat ttctcccttc aaacaaaacc gattttgaaa gtgaaacagt
    30361 tcttactttt tcagtcacaa atgactagag tttccgaaat tttacaattt tcaaataaac
    30421 tctgagcaaa tttcttgcaa gttcttttgt tttgttgtaa tatattttat aacaacgaga
    30481 gagttctcga aattttaaga aaaaggagac acatcatgtc taaaaaagta ttatttatcg
    30541 tcggatcact acgtcaaggt tctttcaacc accaaatggc gctcgaagct gagaaagctc
    30601 ttgctggtaa agcggaagtt agctaccttg attattcagc ccttcctctc ttcagccaag
    30661 atttggaagt tccaacacat ccagctgtag ctgctgctcg tgaagcagtt ctcgttgcgg
    30721 atgctatctg gattttctct ccagtctaca acttctctat ccctggtaca gtgaaaaact
    30781 tgcttgactg gctatctcgt gcccttgact tgtctgatac acgtggcgtt tctgcccttc
    30841 aagacaagtt tgtcacagta tcatctgtag ccaatgcagg gcacgatcaa cttttcgcta
    30901 tctacaaaga cctcttgcca tttatccgta cacaaggcgt tggtgatttc actgctgcac
    30961 gtgttaatga ctctgcctgg gcagacggaa aattggttct tgaagaaaca gtcctaaact
    31021 cacttgaaaa acaagctcaa gacttggtcg aagctatcaa gtaactaaca ctcaataaaa
    31081 atcaaaaagc aaactaggaa gctagccgca agctgctcaa agcactgctt tgaggttgta
    31141 gatagaactg acgaagtcag tgacatatat acggtaaggc gacgctgacg tggcttgaag
    31201 agattttcga agagtataac ataataaaag cgaacaagac tacttttctg acactcagaa
    31261 gactagcttg ttcgcttttt ttctcattta taaactaaaa cagctgatga tacatattgc
    31321 caaatacaaa atatctgatt tctatacttc ctttacaaaa accaatccct gtggttgtga
    31381 caaatcttct cggtaaacaa atccagcaaa gttcaagcga cgtgcttccc ccacagtttg
    31441 tacttgattt tctattaaag ctgttagaaa ggccccgcgc gctttcttgg agatagttga
    31501 gtgaatcttc agctgaccgc ctctatcctc catgaatttg aaggtcacca tctttgctct
    31561 gatttcctta gaaaatacag tctcaaactc tgatgacaag agagagaaaa tcacttcttc
    31621 cttcttcaga gcttcatcat agactgcctt ccaatggctc ttcaaagtct taccagcgac
    31681 ttttaatttc atcaaaaaat ccaaacggtg aggagccatg ggtgacaagg ctggaacaac
    31741 accgtacaaa gccgaggtaa tgaagacatg attttcaaga taatcttgtt ccgcctcggt
    31801 cagcttatct ctcttaatgt tgcggtacat aagcccatca aaaagtttca aggctggata
    31861 gtgttgagca gtttgccttt tcaaagcttg gatattttga aattcttccg ccgctttctc
    31921 agccgatacc ttgtagaaac tctccaattg actggcagaa tagagagcca aggcatcaag
    31981 cacggtctga ctttctggtt ttaaaggaat tgcctcgata cttggcaagt ctgtgttcat
    32041 ttcttttgct gttgggatta aaattttcat attgatagtg tagcatattt tttagccaca
    32101 accaagaaat tcatttgaaa aacctcggtt tgaccgaggt ttttctattt atttgggcca
    32161 tcctaaccag acagccacga gaacaagggc tgtgccagct aaacttgtta agatagataa
    32221 gaatttatct tttttctcct tgaactgaga aaaacctatc ttcaaagcga gcagtccgag
    32281 tagcattgaa gcaaccatca aataaaaacc catttctttg tcctccatta ggcttaattt
    32341 accttattat aacatatatt tattaaataa acctttttta tactcaatga aaatcaaaga
    32401 gcaaactagg aaactagccg caggctgctc aaaacactgt tttgaggttg cagatagagc
    32461 tagagatttt cgaagagtat tattgtactt taaaggtctt gagataattg tcttttccta
    32521 cttgaccttc gaaggtttta ccattttcaa ggtaaggaag gtcatcggat actgaagcct
    32581 tgaccttgta tatcttgcca tcaactttaa agaagtagac agtgtctccc ttgataacag
    32641 ctgatttgag gtctgctact actcccttga tgctttctgt cgttgcattg tcaatttcaa
    32701 ggtcgttttt attggcatac ttgctgagca tctcttccac tgtagtagca acgataacat
    32761 tttggtactc gactgcgtct accagggcgt actctttgac caagccagca ttgtccttta
    32821 agcccatgat gtagagaggc ttgtcattga ggttgatgag gattgggaag gttgctttgt
    32881 aggatttctc ctgaacagca ccttctgctg attcacgggc tgattcttct gtcgcagaag
    32941 ccaagctata cttagtgatt tctcctgttc gcatattttc aaggatgaaa ccaagattac
    33001 tctcatccgc attagccgac gtcacacctg tgtagagata gatgtcatta ccgatagaca
    33061 agtaattata gcccttggta gtctgggtca catttttctt ggaaatcatg gcattcaaga
    33121 aaccgtcctt gtacttgccg ttgtagttga tttgctcaat ggtttcctct gctggataga
    33181 ccctgtccac ccattctgga acatctgata agctgtattc cttggtttct ccatttgtag
    33241 catccaagat aatgactgaa gcaggacgag gaaccgcaag tccaaattgc ttttggtaaa
    33301 ccgttgctac atagaaagga ttgccctcat cgtccacctc aaaagatgga gttttaaaga
    33361 ttttagtcgg gtacttcaag cgcaggtgac gtttgacatc acggttaaaa tactccgagt
    33421 ctgaatactt gattggtgtc ttcaagtcca ccaaatccgc atttccagtt accatgtcca
    33481 ccttgatata ctcaccgatt cccttggctt gattgttaaa ccatttgata gggtctgcat
    33541 attctagtgg tgtgacccga taaggtttcc catcaattgt caattgggta taggtatctg
    33601 ccgctacgta ttgcgacacc ttatcggtta gggaacccaa gtagcggtct ccaatttttt
    33661 cagcagtact tctatctagg ataggaacct tactggtgtc actcctagga aattcagtaa
    33721 agtctttttc cgtaaccgtg actacattgg cataattttt agcctgaaac atgctggaag
    33781 ttaccaagga aaccaaagct gccaagagaa gaattcctcc aatcgaagct aacagaattt
    33841 tccctaaccg attgattttg aaaccctcaa gatttaaggc agcttccgcc ttaccgtggc
    33901 gcacatgaac cgttttgaca aggtttatcc ccttgccaaa gccaaataag attgccacaa
    33961 ctaacaaatg cccacagagg aagaagagaa attcccagct ggtcaggtta aggggcggta
    34021 aaaagatata ccaggtcgtt gcgataaaaa taagttcaaa gactattcgt ttcatttgct
    34081 ttcctcctaa atgattcgtc cttccaagtg atccagctca tgctggcaaa tctgagctgg
    34141 gaagcctgtc aaggtaatgg tctgttcctg ccacttgctg tcacgatagg caagccttat
    34201 ggtttcataa cgcttagttg atctcacacc taccaaggac aaacagcctt cttctgcctc
    34261 ataagatcct tcaaaggaca gaagcactgg gttaaacatg accacgggaa ccaagccaag
    34321 attaaaccca aatcattcat acctctctca actagatgta acttacaaaa cccctgacct
    34381 catgagccac tttcttcctc ctcatgag
//
LOCUS       DAVKJJ010000020        34014 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000020 DAVKJJ010000000
VERSION     DAVKJJ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34014
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.020"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(65..633)
                     /locus_tag="VW144_000942"
                     /pseudo
     CDS             complement(65..633)
                     /locus_tag="VW144_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(709..1299)
                     /locus_tag="VW144_000943"
                     /pseudo
     CDS             complement(709..1299)
                     /locus_tag="VW144_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457791.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1223..1417)
                     /locus_tag="VW144_000944"
     CDS             complement(1223..1417)
                     /locus_tag="VW144_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9600407.1"
                     /translation="MKIIQQQSATIDSLTNELALLREQVAYLTQKLYGKSSEKSVCPS
                     GQLNLFEEESPSEEDGDVPS"
     gene            complement(1614..1937)
                     /gene="tnpB"
                     /locus_tag="VW144_000945"
     CDS             complement(1614..1937)
                     /gene="tnpB"
                     /locus_tag="VW144_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9600408.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENGRLTWPSTEKDVKALTPEQVDWLMKGFS
                     ITPKI"
     gene            complement(1918..2103)
                     /locus_tag="VW144_000946"
     CDS             complement(1918..2103)
                     /locus_tag="VW144_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600409.1"
                     /translation="MPQPIVPVEIPQSRPFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
     gene            complement(2407..3969)
                     /gene="guaA"
                     /locus_tag="VW144_000947"
     CDS             complement(2407..3969)
                     /gene="guaA"
                     /locus_tag="VW144_000947"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU9600410.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            4111..4809
                     /locus_tag="VW144_000948"
     CDS             4111..4809
                     /locus_tag="VW144_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9600411.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQISYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(4852..5748)
                     /locus_tag="VW144_000949"
     CDS             complement(4852..5748)
                     /locus_tag="VW144_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9600412.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(5757..6692)
                     /locus_tag="VW144_000950"
     CDS             complement(5757..6692)
                     /locus_tag="VW144_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEU9600413.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSAPEH
                     GLQTEAVLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(6689..7414)
                     /locus_tag="VW144_000951"
     CDS             complement(6689..7414)
                     /locus_tag="VW144_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEU9600414.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDIASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(7498..8133)
                     /locus_tag="VW144_000952"
     CDS             complement(7498..8133)
                     /locus_tag="VW144_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEU9600415.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEREEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(8135..8962)
                     /locus_tag="VW144_000953"
     CDS             complement(8135..8962)
                     /locus_tag="VW144_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9600416.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(8984..9910)
                     /locus_tag="VW144_000954"
                     /pseudo
     CDS             complement(8984..9910)
                     /locus_tag="VW144_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809127.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            10561..11172
                     /locus_tag="VW144_000955"
     CDS             10561..11172
                     /locus_tag="VW144_000955"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9600417.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRTVAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            11217..11945
                     /gene="rlmB"
                     /locus_tag="VW144_000956"
     CDS             11217..11945
                     /gene="rlmB"
                     /locus_tag="VW144_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU9600418.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(11984..12895)
                     /gene="trxB"
                     /locus_tag="VW144_000957"
     CDS             complement(11984..12895)
                     /gene="trxB"
                     /locus_tag="VW144_000957"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU9600419.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLIVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(12961..13185)
                     /locus_tag="VW144_000958"
     CDS             complement(12961..13185)
                     /locus_tag="VW144_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEU9600420.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTAKARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(13263..14006)
                     /locus_tag="VW144_000959"
     CDS             complement(13263..14006)
                     /locus_tag="VW144_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9600421.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(14006..14806)
                     /locus_tag="VW144_000960"
     CDS             complement(14006..14806)
                     /locus_tag="VW144_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9600422.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(14964..15320)
                     /locus_tag="VW144_000961"
     CDS             complement(14964..15320)
                     /locus_tag="VW144_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU9600423.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(15333..15845)
                     /locus_tag="VW144_000962"
     CDS             complement(15333..15845)
                     /locus_tag="VW144_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU9600424.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(15845..16339)
                     /locus_tag="VW144_000963"
     CDS             complement(15845..16339)
                     /locus_tag="VW144_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9600425.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            16474..16920
                     /locus_tag="VW144_000964"
     CDS             16474..16920
                     /locus_tag="VW144_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEU9600426.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            16917..17564
                     /locus_tag="VW144_000965"
     CDS             16917..17564
                     /locus_tag="VW144_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEU9600427.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(17585..18166)
                     /gene="pdxT"
                     /locus_tag="VW144_000966"
     CDS             complement(17585..18166)
                     /gene="pdxT"
                     /locus_tag="VW144_000966"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU9600428.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(18167..19042)
                     /gene="pdxS"
                     /locus_tag="VW144_000967"
     CDS             complement(18167..19042)
                     /gene="pdxS"
                     /locus_tag="VW144_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU9600429.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVGAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(19194..20573)
                     /locus_tag="VW144_000968"
     CDS             complement(19194..20573)
                     /locus_tag="VW144_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU9600430.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALAGGKIELFRNGAF
                     LVDKKQETSIPDVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            20867..21790
                     /locus_tag="VW144_000969"
     CDS             20867..21790
                     /locus_tag="VW144_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU9600431.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            21850..22455
                     /locus_tag="VW144_000970"
     CDS             21850..22455
                     /locus_tag="VW144_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9600432.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFTDKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEEFDWENL"
     gene            22474..23718
                     /locus_tag="VW144_000971"
     CDS             22474..23718
                     /locus_tag="VW144_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9600433.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AFTGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(23941..24198)
                     /locus_tag="VW144_000972"
     CDS             complement(23941..24198)
                     /locus_tag="VW144_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEU9600434.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(24240..26276)
                     /locus_tag="VW144_000973"
     CDS             complement(24240..26276)
                     /locus_tag="VW144_000973"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEU9600435.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKASLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(26488..27405)
                     /gene="glyQ"
                     /locus_tag="VW144_000974"
     CDS             complement(26488..27405)
                     /gene="glyQ"
                     /locus_tag="VW144_000974"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU9600436.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISNQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            complement(27600..28361)
                     /locus_tag="VW144_000975"
     CDS             complement(27600..28361)
                     /locus_tag="VW144_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9600437.1"
                     /translation="MENLYLVKDDSQLAAFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSNFNAATQIIRESAVPAYTNNRRMVMTPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWLSFLIVDKLVENLG
                     SVQAVFDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            complement(28634..29476)
                     /locus_tag="VW144_000976"
     CDS             complement(28634..29476)
                     /locus_tag="VW144_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU9600438.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     VAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(29590..30981)
                     /locus_tag="VW144_000977"
     CDS             complement(29590..30981)
                     /locus_tag="VW144_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU9600439.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(31216..31593)
                     /locus_tag="VW144_000978"
     CDS             complement(31216..31593)
                     /locus_tag="VW144_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU9600440.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCIQVNQNLEKIRQREGDTHQSYKQIALV
                     YAIAIVDSNYFSDDLAFHSFIVK"
     gene            31808..32785
                     /locus_tag="VW144_000979"
     CDS             31808..32785
                     /locus_tag="VW144_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9600441.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFASGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            32782..33864
                     /locus_tag="VW144_000980"
     CDS             32782..33864
                     /locus_tag="VW144_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9600442.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
BASE COUNT        10068 a         7604 c         6123 g        10219 t
ORIGIN      
        1 aggaaaattg atttatagaa atgttttagc agtcgatgcg tactgttata gattcaacgc
       61 actataattg atgacgttta gctgtttcca acaaactcat aataatagct cttgctttag
      121 ctccttcaaa actttgagaa aataaccacc ctcgcttttt taaactgagc ttaggctaaa
      181 agagtccact ggactctttt actccgtccc ataaccaatg atttaatggc gcattcagct
      241 agattattgg aaaggaccag atgtccgtct ttcaaaatag tcttaaaggt ttctttatac
      301 ttgaggctat acttgaggct gtattcaatt gcccttccta gttttgaacc cgataaaact
      361 gactgacgcc ggcaccaagc aaagaaatct tccattaggg gttggagctc ttcttgacgt
      421 ttctgtagtc gttcatcagc tggcaaagcc tcccagtttc tttccaagga aaataactga
      481 ttacaataag ctaaaccttt agctcctaag gatgatttat tcgcttgctt gggggtcgct
      541 tcaaaaaact tccttctcac atgtacccaa catccaacaa gttcagcttc ttccagttga
      601 ctgacgataa gcttctagcg gttcgcagtt taccaagctt agcgtcgcct tgctcctaaa
      661 ccttggactg ctatcgcata ggcagaacta gaggactaaa gtcctaagtt actgccgcaa
      721 catgtcacaa tgcacatagc cagaataatc tcctaggaat tcttgtacta ctaaaccact
      781 ccgacgctga tcatggtggt acagcgtgat tgcttgattt tcagctttcc cagacaaaaa
      841 ggtccaatag taggtcagct gactatcact ctctagcacc ctataagaag tttcatctgc
      901 atgaagtaag ggcttgagtc aatagtctcg ttataaaggg gctttaggaa taggagcttt
      961 cacgatttta tcactcggat ttttatcact gcatgcttgg catttgtaag cgtgttggat
     1021 atgatctatg cgttttaatt gcgcaggaat aaagaccaat tcttgtcctt gaagactccc
     1081 tccaatctct tttagatctc cctgacaatc agggcaaatc ctctcttcta cttgatgatg
     1141 aacttcttct gaatcaaatt gggcaagaag agcttggcgt ttccctttag atttcttacg
     1201 tttgtaggta atttcttctg tttcaactgg gaacatctcc atcttcctca gatggagact
     1261 cttcctcaaa aaggttgagt tgtccagagg ggcaaacact tttctcagag gattttccat
     1321 agagcttttg agtcagataa gccacttgtt cacgcagaag ggcaagttca ttggtgagac
     1381 tatcaattgt agcactctgt tgttgaatga ttttcaatct tcttccatga gcttccctct
     1441 ctgttttcta actttattat actaaaaaga aagtcctcat ttcaatagaa atcacgactt
     1501 tctgatggat ttatagtaaa atgaaataag aacaggacaa atcgatcagg acaatcaaat
     1561 cgatttctaa caatgtttta gaagcagagg tgtactattc tagtttcaat ctactatatt
     1621 tttggagtga tagaaaagcc cttcataagc caatctactt gttcaggtgt gagagctttg
     1681 acatcctttt ctgtactggg ccaagtcagt ctgccgttct caaagcgttt atatagtagc
     1741 caaaatcctt gaccatccca gtaaagggct ttaaagcggt ctttacgtcc accacaaaag
     1801 agaaaaactt gaccggagaa aggatccaat tcaaagtggg ttttaaccag ataagccagt
     1861 gaatcgattc cttgtctcat atcagtcttc ccacacacga gatagacctg ccctaggcta
     1921 gatagatgaa ttgtcataga gcaacacctt atccaaaagc tgttctacca tttcgagatt
     1981 gagagattga aaaaaactta cttcaagctt gccaatacga attttaagca gaatatcatt
     2041 tctcttttta gaatcaaaag gacgagattg tggaatctct acaggaacaa taggttgtgg
     2101 cattaaaata tcctccaata gttttacttt actaatagta tacaggagga actccatgaa
     2161 tgatagatac cttgttatga cgcgcttact atattagaaa gttggctctc taattaaatt
     2221 aattctcatt atatatatca aaagaccgca gtttgaaaac tacggtctaa ttttttgtcc
     2281 aaatgtaaat cacttggaac tattattcaa tttattcttt ttagtaaatg ttcccgtttt
     2341 aatcccagtt acagttttta atcctctgta accgttgatt ttacaaagct tttaattttt
     2401 ttgcgattac tcccactcaa ctgttgcagg tggtttactt gtaatatcgt agacgatacg
     2461 gttaacatga tccacttcat ttacgatacg tactgagatt ttttgaagta cttcccatgg
     2521 aattttggca aaatcagcag tcataccatc gatagaagtg atagcacgga ttgcaatcgt
     2581 gtagtcatac gtacgaccgt cacccataac accgactgaa cgaacgcctg tgttaacagt
     2641 gaagtattgc caaatatcgc ggtcaagtcc agctttagcg atttcttcac gaagaatagc
     2701 gtctgattca cgaacggttt caagtttctc ttcagtgatt tcacccatga cacggatagc
     2761 aagtcctggt cctgggaatg gttggcgcca tacgatatgg tctggcatac caagctctgt
     2821 accaagagca cgaacttcat ccttgtaaag agtattgagt ggttcaatca attcaaactg
     2881 catatcttct ggaagaccac ccacgttgtg gtgtgacttg atagtttgag ctgtatccgt
     2941 accagactcg ataacatctg tatataaagt accttgagca aggaatttca catctttgag
     3001 cttgcttgct tcgtcatcga atacatagac aaactcgtta ccaatgattt tacgtttttg
     3061 ttcaggatca gaaacgccag caagtttgtc aaggaaacgt ttagcagcgt ctgctttgac
     3121 gatattcaaa ccaaacttac caccgagcat gtccataact tgatcagctt cgcctttacg
     3181 aagaagaccg tggtctacga agatacagat caattgatcg ccaatcgctt tttggagaag
     3241 aaccccaacg acagatgagt caacaccacc tgatagacca agaaggacac gtttatcacc
     3301 gacggtttca cgaatttttt tgatctgcat gtcaatgaaa ttatccattg accagtcacc
     3361 tttagcctta caaatgttaa gggcaaagtt acgaaggata tcatttccgt atacagaatg
     3421 acgaacttct gggtggaatt ggataccgta aatgtgttta tctgggtttt cgatggctgc
     3481 gtatgggcag tcagctgatg tacctgtacg aacaaagtca gcaggaatct cagtaaccgc
     3541 atcaccatgg ctcatcaaaa cagtctgttc atcaggtgtt gattcaaaaa gcgctgatgg
     3601 tgtgtgagtt agggttgatt gaccgtattc acgatttcca gcatcacctg caggaacaac
     3661 ttttcctcca agtttatggg tcaataactg cataccataa cagattccca aaattggaat
     3721 tccgagttcg aagatttctg ggtcaatatc aaatgaacca tcttcatata cagaatttgg
     3781 accacctgat agaataattc ctacaggatt gacttcacga acttcagcag ctgaaatttt
     3841 atggcttttt agttctgaaa aaacaccaat ctcacggata cggcgtgaaa tcagctggtt
     3901 gtactggcta ccatagtcca atacgatgat tttttctaca tcttgcaaat cagttgaaat
     3961 gttgctcatc tttttccttt ctcaaaagaa atatcttttc tcaatattct atcacaaata
     4021 agggcgaatt accaactaaa caaggcgaag tttgactttt tctagtttat tggggtacaa
     4081 tagagaaaag atagaaatga agtgaaaaat atgctacctg cttatatgaa aatccatgat
     4141 cagataaaaa aggatattga cgagcaccgt tgggctattg gtgagaggct tcccagtgaa
     4201 agagatttag ctgagcagtt tgcggtcagt cgcatgaccc tccgccaagc cgtatctcta
     4261 ttagtcgaag aaggcgtctt agagcgccgt gtaggaagcg gcacctttgt ttccagtact
     4321 cgagtacaag aaaagatgcg agggacaacc agttttactg aaattgtcaa atcccaaggt
     4381 aaagttccct ctagccagct catttcctac aaaaaaacca ttcccaatga gcaagaagtt
     4441 gccaagctag gaatttttcc aacggacaat attatccgaa tggagcgggt ccgctatgcc
     4501 gaccaagttc ccctagttta tgaagttgct tctattcctg aaaaattcat taaggacttt
     4561 aaaaaagaag aaatcaccag tcatttcttc caaaccttgc aaaaacatgg ctatcgtatc
     4621 ggtaaatccc aacagaccat ctatgctcgc cttgctaaag aaaagattgc ccactatttg
     4681 gaagttgaaa aaggacatgc tattcttgga ttgacccaga tttcctacct agaagatggt
     4741 actgcttttg aatacgtaaa aagtcaatat gtaggcgaac gctttgaatt ttatcttgaa
     4801 aataattaga ttaccacgca aaaactctct gtcacggaca aagagtcttt tttatttttc
     4861 taagaataaa tctttcaaag aaatcaaagt tacagcaaac aatatcattg ccataccaag
     4921 aaaatcaatg ggataaaatt gttcattcat tattaagaag gcaaagaaaa tcgccgatat
     4981 tggctcaatt gaagccaaca agcttgactt gaccggtcct atcagactgg ctcctttaag
     5041 gaaagctgta taggcaaaga cagtcccgat aaggataatg cctgcaaacg caaggagaaa
     5101 atcaagacta gtcgggatat cggcctgtag aacccctgta aaaggaaggg cgaccaaacc
     5161 tgctatgacc attcccacac caatgaccaa gctgctcccc cactttttaa tcaaggctat
     5221 gggtaaaatg atatacagag cataagtcaa ggcagaaaag agaccccaga acagaccagc
     5281 aggtgtcatg gataactggt ccaactgccc atgtgttgcg atcaggaagg ttcctccgat
     5341 ggcgaatatg atggaaacta tctctcccag tgtcggtgcc accctatcct tgatacagct
     5401 ataaattaaa attccgacag gacaaacata ctgaagcacc gtcgctgttc ccgcattggt
     5461 ctcctgaata gcagacagat aggcgaattg gttgagaaaa agaccaatca gagcaaaaat
     5521 aagaagagac agcaaactct ttctatcctt taaaaagacc agtattttat cctttgcagt
     5581 agcataagcc aagagcatga gaattccacc agcgattaaa agacgcaagt tagtcaagac
     5641 cagagccgaa attccgtgtg ccattaggta ttggccactc gttcctgaca acccccaagc
     5701 aataccagcc acaactgtta gtaaagtccc ttttaaacta tttgacatgt ctctccttac
     5761 tcttctttgc gaatcaagtt catcacttga ttgcggtcta atatccattg agctctctcg
     5821 tccttttcat aggtacgatt cgataggaaa atagtggctt cttgcttctg acgattccac
     5881 atgataaagg tacctgtata gcccgtatgg tctagccaat ctccttccaa attccatgct
     5941 aaagaacgtt ccttgtcatc caaaggagaa aaattttgat ttaagtctct tgcaaaatca
     6001 tctgctaaat agtgttctaa aaagatttgt aaatccttta tagtcgaaaa taaaccagca
     6061 ctcccagcat gtctacccag gagacgagcc ttgggatcat gcactatgcc tgcctctaca
     6121 cctctaactg ttggaacagc aagctcaact ggcccaaact tggtttccgt cattccccaa
     6181 ggtttccaga cttgatcctt taaaatcaca tccaaatctt gattaaaaat tctttccaaa
     6241 ataaagccca acagcaaaaa atggacatcc gaataaagaa aggctggctg acttcgtctg
     6301 ttgagatgaa acatcgcttc ctttaattca ggggctgtta aaagatcacg attaggaata
     6361 aaaggatcaa ggtctgttgc atgagtcaag agctggcgaa tagtgatgtc tggataatca
     6421 ctctcaggta aaaaatctat taccagtcta tcaatatcta attgacctat ttcccacaag
     6481 aaggtacaaa ctgtgccaac cccaacaacc ttgctgacac tagctaggtc ataaactagt
     6541 actgcctcag tctgcaagcc atgctctggg gcactctggc ctaaatagaa ctctgtccat
     6601 tgattgtcct taaaatacgc aaaagaggct ccgggataaa ttcctgcctc gatttgttct
     6661 tctatttttt taataatctt ggtccacttc atacttcttc aaaccacaat tcaacattat
     6721 ttctatcttt accaaggaag aatttttcag acttaggaat aaaatattca gtagactcaa
     6781 acttctggcg aagacttgct atgtctaatt cattgaccaa gaacttgagc atagataagt
     6841 cccaggtaac cgtattgtcc acagtcaaat cctgcccctg agctggaata aaatcaaggg
     6901 atgccccaat ttcagatgat tccaagaaac tttcgatatc agttgggaga tgtaattcca
     6961 tagaaatctc aaatttactt aaagaaattg atgccaaatc tgtttgaaat tcaggctttt
     7021 ctcctacttc tactagactt gctatgtcat cttccgcatg aatcaaaatc aaatcatctt
     7081 ctggtgagaa aatttcaaaa gcgtagccat tttgaccttt atataatcga tgaatcgaat
     7141 ctgttttaga taagattcct tcaatttcta agggattttc caccttgaca atcaatctag
     7201 ctagtttttt tcttccctct accttacgag tacgcatact gggagcttct tctaaaacca
     7261 gcttttcaag acccgtttgg tcacctagtg acagaaaggc cgattcttct aacaaggcct
     7321 tcattccaag ggtttcaata taaaatgttt catttaattt tctattatta gcttttaaag
     7381 taggaataat ccgtacaatc tgatttacat tcataaattc ctccgtcact ttttatttta
     7441 aaggatttta gaatttttta caagatatta tgctcaaatt taacattatt tagctattta
     7501 gtaaagatag tctttcaagg attttgacaa agcctgataa ccagcaatac tgaggtgcag
     7561 tccatcagtt gtatattctt ttttgagttg gcctgcttgg tctgtcaaac aatcaaatac
     7621 tggcacaaat tccacctgca tataggcaga tgcaagctct tgataggctt gattccagtt
     7681 ctgaattttt tcattcgagc ggatatagac tgcctgctgg tactcctctc tctcattgac
     7741 aggcaaaatg gaaagcaatt taatctctgt caatggataa tcgcgagcaa cggattgaat
     7801 gatagcttcg agattattga gagcctcatt cacaggaaca tcctttccga tatcatttgt
     7861 cccaatcaga agaaaaattt tatctactgc tccaccatat agatgagcat caaggttctc
     7921 tagtaacagt cctgtctgat agccacgaat tcctcgattg acaatcgtct ttgaagtccc
     7981 aaatagctcc tgtagaggat aatactcgac aatggaatcc ccaataaaaa gaatgtttgg
     8041 ttctacaaca gaaatgtgat ttaggtgacg atacttagtt tgaatttttt cttgttcctt
     8101 taggagccaa ttttctaata actgtactgc cacttcattc tcctttttct aaccagtttt
     8161 ccaagacttg ataaactcct ccttggctgt tggctggtgc tagagcagtc gccacagctt
     8221 tggctttctc atcagcattt tccatcgcat aggcaattcc agccatttca agcatttcaa
     8281 catcattttc actatcacca aaagccatga tttcttggga tttcaagtcc cagcgcttga
     8341 gtaattcctc caagccccat gctttatgaa tcccagcttg gaggatatca atgcaaccat
     8401 agccactgga tacagctcgg acacggccat caaagagagc attgatttct tccaaaaccg
     8461 aactcaaacg ttcctcacca acaaccatgc tcatcttgag cacaccacca aagaggtcag
     8521 atgttaattc atccacaaat tgcatccgtt ggtagaattt ttcaatcatt tctggagtca
     8581 taaaactttc aagatctgta aaaatcgtac cttccttgac aaaatcaccc ttcatccccg
     8641 ttacaacaaa ctggtcctga cacgctcgac ccttgaaatg agtcaaagcc ttgttgacaa
     8701 tggcgtcatc ccatgtctga gcctgaatca attcattgtt ttcaaaaata cgagcgccat
     8761 tagcaacaac cagaaccact cgatccacca agggactcag tagttgtctc atgcggtgaa
     8821 tttcattgcc cgtcgcaatg acaaaacgaa tgcccctttg atccaactga tctaaaatct
     8881 tttccaatcg tgggagatca agctgacctc tagcatccag caaggtccca tccatatctg
     8941 tcgcaattac cttaatcatc atttactttc ctctggattc aagctaatac cacttcaatc
     9001 ccacatgttc attcatttct ttataggctg tatcaattcg ttccttagtc gcttcatcta
     9061 actggtcacg atgactgcca tcctctataa aatcttccaa aatataagtt tggtaatgat
     9121 acaagggata tccttctggt gaaaaggttc cctttggtgt tcgattgacc caagtaggat
     9181 gagctttagc tgttccgata gttgtttttc catccttctt cttgatggtg acatccatga
     9241 gaacaccacg ttcagtccac ttagcattct cttcatctcc catagattca attcgttgat
     9301 tggaaatgaa gttccccatt taatagataa tgagtttctt atctccatct ttttcaaccg
     9361 tttcagatgg ttcaacaacg tgaggatgcc ctccaaagat aatatccgct cccaaatcga
     9421 tcatcttgtg ataaagagct ttttgttctt cagttggttc caatcgatac tcaacaccca
     9481 tctgaagcat gataatggtg atatctgctt ccttctctgt ccgttcaatt tcaaccttca
     9541 tcttatcttc gtttaaatct gaaagataac gattatagtc ttcctgagaa atatactgct
     9601 caattccatt gaaaccatag gaataggcta acaatgcaac cttgatacca ttcacttcct
     9661 taatgaccag cggagcctga tcacgtggtt cgtgcgtata aactccgatt ggagtgattc
     9721 cagctttctc aataatatcg gccgttgaaa taactccctc aatttgcgaa tccaaaatat
     9781 gattatgagc taaatccagc acatgataac ctgcctcctt aatagcatcc ataacttcaa
     9841 caggagcatt aaagagaaga taacccgcta aataatgatc cttattaatg gttccttcaa
     9901 aatcaccaat agctaaatct gctgcttagt ccccttgctt gagccaagga gtcacatact
     9961 caaaattttc atgaaagtca taggtaccgt cttctttttt agctgaaaag aaaagtccat
    10021 cgtggtagag caaatccccg tgggccataa ttctggcagt tttttcctct tcctactcct
    10081 gagacttttg cttagtcccc tcttgagaaa tagtatctcg tttttgacta gtcaagggat
    10141 tccttggaag ctttcaaaca acaagaccaa gcccattgat aaaccaactg ctagcaagag
    10201 tatcgccaca aatcccttat tgctccactt gcgataactc ctaaaaagtt taccaagccc
    10261 ttcataaaac gaaaagctaa accaccctga tttcgatttt gtcttctttg tatcttcgtt
    10321 ctccctactt tcttatgcaa gccttttctt tttattatat cacagataag tatttctttc
    10381 acaattgaat tgaacttccc atctattttc tataaatcct aaatgccata atgctttcaa
    10441 ttcttgtcat tttgtgatat catgtagagg aaatgaacta atccacagtg gcttattcca
    10501 agtataccac ttgggctttg gcagtagcta actgcactaa acataatata aggagagaaa
    10561 atgtctgcaa tagaacgtat tacaaaagct gctcacttaa ttgatatgaa cgatattatc
    10621 cgtgaaggga atcctactct acgcacggtt gctgaggaag tcactttccc cctatctgac
    10681 caggaaatca tcctaggcga aaagatgatg caattcctta aacattccca agatcctgtc
    10741 atggctgaaa aaatgggact ccgcggtggt gttggactgg ctgctcccca gttagatatc
    10801 tcaaaacgca ttatcgctgt tttggtacct aatattgttg aagaaggcga aactccacag
    10861 gaagcctacg atttggaagc cattatgtac aatccaaaaa tcgtctctca ctctgttcaa
    10921 gatgctgctc ttggcgaagg agaaggttgc ctgtctgttg accgtaacgt gcctggctat
    10981 gttgttcgcc atgcccgcgt tactgttgac tactttgaca aagatggaga aaaacaccgt
    11041 atcaaactca aaggctacaa ctccattgtt gttcagcatg aaattgacca cattaacggt
    11101 atcatgtttt acgatcgcat caatgaaaaa gacccatttg cagttaaaga tggtttactg
    11161 attcttgaat aaagaaaatc ccgttgcaag acggggtttt gtgttataat agaggtatga
    11221 aaacaaatga tattgtctat ggtgtccacg ccgttaccga agctctcctt gcaaatacag
    11281 gaaacaaact ctacctccaa gaagatctcc gaggtaagaa tgttgagaaa gtcaaggaac
    11341 tagctacaga aaagaaggtg tccatttctt ggacatcaaa aaaatctctc tctgagatga
    11401 ctgaaggtgc tgttcatcaa ggttttgttc tacgagtgtc tgaatttgcc tatagcgagc
    11461 tagattacat ccttgcaaaa acacgccaag aagaaaatcc acttctattg attctagatg
    11521 gtctaaccga tccccataat ctgggttcta tcttgcgaac agccgatgcg accaatgttt
    11581 caggtgtcat cattcccaag caccgtgctg tcggagtaac tcctgtcgtt gccaaaacag
    11641 ccacaggtgc tattgaacac gttccaattg cccgagtgac aaacctcagt caaaccttag
    11701 ataaactcaa ggatgaaggc ttctggacct ttggaacgga tatgaacggt actccttgcc
    11761 acaagtggaa tacaaaaggg aaaatcgccc tcatcattgg aaatgaagga aaaggtatct
    11821 ctagcaacat caaaaaacaa gtcgatgaga tgattaccat tccgatgaat ggacatgttc
    11881 aaagccttaa tgccagtgtt gctgcggcca ttctcatgta cgaagttttc cgaaatagac
    11941 tataaaaaag tttccagtca tctgattgga aactttttta tgattaacta tgttctgtga
    12001 taaacttgta ggcttcttga ccagcgatag ctccatctcc aactgctgtt gttacttggc
    12061 gaaggtcttt caagcgaaca tctccaactg caaagatacc gtcgactgca gtcttcatgt
    12121 ggctatctgt cacaatccag cctgcctgat cttggatatt caattcttta acaaaatcgc
    12181 taagagggtc caaaccaaca tagataaaga caccaccgaa ggcttgttct gtcacttgac
    12241 ctgttttcac attttcaaat acgactgatt ctactcggtt ttcacccttg atttccctta
    12301 ctacagaatc ccagataaag ctgatttttt cattcgcaaa ggcgcgatct tgtaaaacct
    12361 tttgggcacg aagttggtca cgacggtgaa caatggtaac agtcttagca aaacgagtca
    12421 agaagagggc ttcttcaaca gctgaatctc caccaccaac tatcaataaa tcttggtcac
    12481 ggaagaaagc accatcacac acagcacagt aagaaacacc acgactgttc agttcttctt
    12541 ctccaggcac tcccaaagga cggtgtttag aaccagttgc tacgataact gtacgtgttt
    12601 catatgtttg gtcatcagtc atcactttct taaaatcacc atggtcttcg acattttcaa
    12661 cataaccata aatgtgctca acaccaagat tttcaagtgg ttcaaacatc ttttcagcca
    12721 attcaggtcc actaatatta gcgtatcctg ggtaattttc gatatcagat gtattattca
    12781 tctgaccacc tggcagacca ccttcaatca aagctacttt tagattgctt cgagcagcat
    12841 acaaggccgc agtcatccct gcaggtccag caccgataat aatagtatcg tacatataga
    12901 ttccttcttt cttgttgtaa ctatctttat tctaactctt tcttgtcaat cacgcaagga
    12961 ttatgccttg aaaacaagaa ttaaactcat aaccgcaaag gataatactg ggacaaccaa
    13021 gactccaatc ataatcatat catagagatg ggcttggcga gccttggctg tcttatcaac
    13081 tcccgacatg gctctcagac caatccaaat ccctaaaaaa atcagaacaa ggatggtggt
    13141 caatatcaaa ctctcgaaat ataaagaaaa tagttgcagt agcatgattt ctctcatttc
    13201 tatctttttt aaagagtaaa ctcagctagt ccaactaact gagttttcct ttatctatta
    13261 tatcaaatat aagtccgttt gtaactagcg aagaattctt ttgtccgctc ttctttaggg
    13321 gtgtggataa tctcatccgg agttccagac tcgatgattt tccccttatc taagaagaga
    13381 atcttatccg caacttgggc tacaaaggac atgtcatgac tgaccaaaat catggtctga
    13441 cctgacttag cagcatctgc aatagacttt tctacttcac cgaccaattc tgggtcaagg
    13501 gctgaagttg gttcgtctaa gagcaaaaca tctggtttca tagcaagcgc acgcgctagg
    13561 gcaacccgtt gcttctgtcc acctgataaa tggcgaggat aatggttttc acggtccgaa
    13621 agcccaacct tagccaactc ttccttggca atcttagtcg cttcttggtc agataatttc
    13681 ttgacaacaa ccaagccttc tttcacatta tcaagtgctg ttcggcgttc aaacaaatta
    13741 aactgttgga aaaccataga caacttacgg cgtagggcaa ggatttcttc ttgagtgatt
    13801 ttagaaaaat caactgaaaa accatcaatc tgaatagagc cactgtcagg tgtttcaaga
    13861 taattgagac tgcgaagaaa ggttgatttt ccagctcctg aagaaccaat caaggctaca
    13921 acttcccctt tttgaatatc caagtccaga tgatccaaga cagtctgtcc tgaaaaggat
    13981 ttgcttaaat tcgaaatctt aatcattaac gaaggtctcc tttcacatct gtttgcactg
    14041 tatcaggtgc agaaatagcc atttttctct cgatgaaacg accgaggctt tcaattccga
    14101 tattgactac ccaataaaca agggcaacgg agatgaagcg ttcaaaatag cgataatcag
    14161 ctccacctag aatctgagct tgggcaaaga cttccacaac acccgcacta aaagctagag
    14221 atgttccctt ggtcaaaccg atgagggaat taatcaaggt tggagtagct accaccgctg
    14281 cattaggaat aatcactcgt cgataaactt gcgctcgggt catacccaaa ctgcgtgccg
    14341 cctcaatctc accaggatta actgagagaa tggctgcacg aatggtttca ctagcataag
    14401 ctgcctcatt aaaggcaaaa gcgacaatcg caaaagctgc agctggaatc gcattgatat
    14461 tgagaccagt tccccattgc tgattgaggg ctttcaaagc caaagggatt ccgtagtagg
    14521 tcaacatgag ttgcaccaaa atcggtgtcc cttttaagaa actaacaaag aaggcctgca
    14581 agggatataa aatcttgaca cgattgatct tcacaatggc aaaaagaagc gccaaaacca
    14641 agccaaaaag ggcaccacca attgtcaaca taattgttgt tggaagttgt tggacaattc
    14701 tagggattcc atcaaagacc gaacgtaggc taaacagctt gccatccgga atcaattgca
    14761 tcaagttttg gtaccaatct gatgctaaaa tcgttgtaac attcataaaa acatcctctt
    14821 ctgataatga ttcattctac catgcttctg ccgtcaatga aattatttgc tacggtcaga
    14881 tacagacttt gtctacctac tagtttatca tactttgaaa cagggaagtt atatatttta
    14941 tctatcaaag agatagataa aaactatttc agtcccagtt cttcataaga ttttcgataa
    15001 ccgatttgct taacagttcc attttctact aaaatgggcc gttttaacaa cataccgtca
    15061 cttgctagca actcagccgc ttcttggttt gacaaacttc ctaccttatc ttttagccct
    15121 aattcacggt atttgatacc actggtgttg aaaaattgct tcaattcaaa tcctgaggtt
    15181 tctagccaat tcaaaatgac ttcttggcta ggtgtttctt ccacgatatg gacggcttta
    15241 tagtccacac ctaattgatt taattcttgt tttgcttttt tacaagttga acatttgggg
    15301 tattcgataa attctaacat gtgcttctcc ttctatttgt ttatatcttt aaaatctacg
    15361 ccttcatgct ccaagagcca agctttcttt tccactcctg cagcatagcc tgtcagacgc
    15421 ttgcctgctc ccaacacacg atgacaaggt actaggatag accaaggatt gcgtcccact
    15481 gctccaccaa ttgcttgagc agaagccact tgcaggtctt gagcaatttg tccataggtc
    15541 actgtctgac cataaggaat gccctgtaaa tagtcccaaa ctctcttttc aaaattcgtt
    15601 ccgattggcg ccaagagcaa gttggataaa tcctgaggct tgcctttaaa gtaatcatct
    15661 aagcaagcaa taactgggtc taaaatagga tgactaacaa cttcttctat cgtttcatct
    15721 cctagtcccc tctcaaaatg cttctgctcc tgaacccaaa ttccatacaa ataatggtca
    15781 tcagctacaa gtgatagaat accaattggt gaggagtaga gtatttttac atacttcttt
    15841 tgcattattt ctttaatttt tgataggcca agcgttcgcc atctactggc accttaccaa
    15901 cttgtttaaa accaagtttt tcaaaaatat gttgcataac cttgttttca gcatgcgtat
    15961 ctgagcgaaa atcaagataa tcaaaacctt caatcaagcc ctctaagaag gtttgagcaa
    16021 ctccttttcc ctgcacatct gctgccacag caatacggtg aaagactaga tactctgact
    16081 ctccagcttg ccagtttccc tcataaatag cttcataggc ctcctctgga ctcttggtca
    16141 cagcagcata ggctagcagt tctccctctt ccaaggctac gtaggcttgc cctgagatga
    16201 tatcatcgat aataacatct gcatttggat agccattttg ccactggtca ctaccagcat
    16261 ctgctaaaca ttttttggca tcctccatca cctgcatgat ggcatctact tcatttggaa
    16321 aagctaaacg aatttccatc ttttttcctc atttctgact agtttctccc atcatagcat
    16381 aatttaagta tttctacaag cagttaaaac ttgactttgt gtttttgtta ttttataatc
    16441 tagttatcaa aactatataa aaaggagttg aaaatgaaaa ctaccttttc ctacccaaaa
    16501 tgggcagaaa ttccaaacat tgacctctat ctggaccagg ttttgctcta tgtcaatcag
    16561 gtctgcgccc ctatctctcc taataaagac aaaggcctaa cagcatctat ggtcaacaat
    16621 tatgtgaaaa atggttacct gacaaagcct gacaaaaaaa aataccaacg ccaacagatt
    16681 gcccgtttga ttgctatcac aaccctcaag tctgtatttt ctattcaaga aatagcccag
    16741 acacttaata ctctacaaac tcaagcaagt tcagaccaac tctacgatgc ttttgtggac
    16801 tacatgaacc aaggaattga cccagctaac cctattatcc aaaccagctg tcaaaccgtt
    16861 aaactctatc atcaaactct agacttaatc gatcatactc aagaggaggt aatccaatga
    16921 acactagtct taaactcagc aaacaactca gttttggaga ggagattgct aatagcgtga
    16981 cccatgctgt gggtgcagtc atcatgctta tcttgctgcc tatttcatcc atctatagtt
    17041 atgaagcaca cggattttta tcctctatcg gcgtttccat tttcgtcatc agtctctttc
    17101 tcatgttcct atcatccacc atttatcact ctatggccta tggttcgacc cacaaatatg
    17161 ttttgcgaat cattgaccat tctatgattt acgttgccat tgccggctca tacacgcccg
    17221 ttgtcttgac cttgatgaat aactggtttg gctatctgat tattgtcatc caatggggaa
    17281 cgaccatctt tggtattctc tataaaatct ttgctaaaaa ggtcaatgag aaatttagcc
    17341 ttgctcttta cctgattatg ggctggttgg ttctggctat cattcctgcc attatcagtc
    17401 aaacgacacc cgttttctgg agtctcatgg taactggcgg actctgttat acagttggag
    17461 ctggatttta tgccaagaaa aaaccttatt tccacatgat ttggcatctc tttatcctag
    17521 ctgcgtccgc acttcaatac atcgctattg tttattacat gtaaaaaagt tgagaaattc
    17581 aatctcaact tttttcttta cacatattga taaagtactg gtgcaagcgc acatcatcag
    17641 tcaattctgg atgaaaagaa cttaccaaca tatttttttc ttgggctgca acaatttgat
    17701 tgttcactgt tgctaaaatt tctacaccct caccaacact actgataatc ggaccacgga
    17761 taaaggtcat tggaatcttg ccaactccct tacattctgc ttccgtgtag aaacttccta
    17821 attggcgccc ataagcatta cgctcgacca ccatatccat agttcctaga tgactctctt
    17881 tctgagaagt gatttcctta gccagcaaaa ttaagcccgc acaggtccca aacactggta
    17941 agccagatag aatggcttct cggatgggaa gtagcatgtt ctggtcacgt aagagcttgc
    18001 ccatggttgt agactcacca ccaggcaaaa tcaaacccga caagtcactc tgatcttgct
    18061 gaaaatcatc tagatttctg agttctacac tctcgacacc taattgatct agcacttttg
    18121 catgttctgc aaaggcccct tgcaaggcca atattccgat tttcatctat tttcctcgtt
    18181 cagccatgag aatttggatt tcattttcat taataccaac catggcttct cctaaatctt
    18241 cagagatttg agctaggatt tgaggattac ggaagttagt cacagcctta acaatggcac
    18301 tcgctcgttt aacaggatct cctgacttga aaatacctga accgacaaag accccctctg
    18361 cccctaattg catcattaac gcagcatctg ctggcgttgc aacacctcca gcagcgaaat
    18421 ttacaactgg caattttcca tgttcatgaa catattggac caattctaca gggacttgca
    18481 aatccttggc accaacataa agctcgtcct cacgtaagtt ttgaatgcgg cgaatttcct
    18541 gattcatcat acgcatatga cgaacagctt ggacgatatc ccctgtccct ggttctcctt
    18601 tggtacgaat catggaagca ccttcagcga tacgacgcaa ggcttcaccc aaatccttag
    18661 caccacagac aaaaggaact tggaattctt tcttgtccac atggaaacgg tcatcagctg
    18721 gagatagaac ttcactctcg tcgatataat caatttcaat agcctctaaa atctgagctt
    18781 caacaaaatg cccgattctg accttagcca ttactggaat actaaccgct tcttggattt
    18841 ccttaatcat ctttgggtcg ctcatgcggg aaactcctcc agctgcacga atatcagccg
    18901 gaattcgttc caaggccatc acagctgccg caccagcagc ttctgcgata cgagcctgtt
    18961 caggattctg cacatccata ataacaccac ccttgagcat ctgtgccaag tttttattta
    19021 gttcataacg attttcagtc atttgtttta tcctcatcat ttgtattggt agctaccttt
    19081 tcattttaag tcagaacaga atgaagcaca agataaaaaa actataattg tatggggaca
    19141 aattagctaa aaactatctg accttaactt aaggccagat agctcattca tttttatttt
    19201 tcagccgtaa gggcagccat tgtgatgtag ttgtatggtt tgttgaagtg tggcaagaag
    19261 aagaggtctg tcaatgccaa tttatcaatt gtcacatgct cttggatagc aagtgagaac
    19321 atgtggattc ccatgctaat tgcaatatca tgtgaaacca tttgggcacc aagaatttca
    19381 cggctatctt tgtcaaagac aatcttaatt gctacttcat ggttgtcatg tttcatgaat
    19441 tctggttttt gaagatcgtt aaagcctgtt tcagttgcgt tgtaaccagc agctttcgct
    19501 ttttcaagag tcaaaccagt tgaaaccatg tgaagaccgt agattgagat accatttgaa
    19561 ccttgaacac cgattccttc caattcatgt ccacaagcat tgtaggcacc aacgatacca
    19621 gtgcgaacag catttgaagc aagagcgata tagcttgtat ctttacgagc attgtcataa
    19681 acagtcgcac agtcaccaac agcgtaaacg tctgggatag atgtttcttg tttcttgtct
    19741 acaaggaagg caccgttgcg gaagagttcg atcttaccac ctgcaagggc tgtgtttgga
    19801 cggaaaccaa ctgcaaggat aaccatatcc acgtcaaagc tttctttgtc agtaatcaag
    19861 cgttcaactt taccgtcacc ttcgattgct ttaacagttt gacctagagc caagcggatg
    19921 ttgtgatctt ccaagttctt cgccatcatt tgtgtgaagt ctttgtcata gtaaccgttc
    19981 aagacagtat caacgatatc aacaaggaca acttcttttc caagacgttc aaaggcttca
    20041 gcaagttcaa caccgatgta accaccacca acaacggcga tacggtcgag gtgttggctc
    20101 ttgtcagaaa gtttattgat aacttcttca gcattttggt acaatttcac gaattgtacg
    20161 ttttcaagag ttgctttaaa ttcgcggttt cctttaacaa tttcaacacc ttcgattggt
    20221 ggcaagattg gtgtagaacc tgtagcgaaa atcaattttt cgtatgattc tttgtgctct
    20281 tttccttcaa cttccgctgt aactactttg ttatcatagt cgattgaaag aacaggtgag
    20341 ttcatgtaaa ctttagcacc tttagcttcc aatttttctt tatcagaata gaacaagcct
    20401 tcagcaccgt caatttgttc accaatccaa agagccattc cacatcctag gaaagagatg
    20461 ttagagtttt ggtcaaatac aacaatttcg ttctcatttc caaaattatc caacatggta
    20521 ttgatacatg ctgtaccagc gtggttagca ccgactacaa cgattttact cataaaaatt
    20581 tcctgccttc attctaaatt taccttacta gtataccatt taccgttagc gtttacaagg
    20641 ggggtactat tatttcagca ttttttatca aaaataattc cccatttatc ctgttttcaa
    20701 gcctgatgaa ttttatgagc atatttcttg actttttcca aattttgttt gtgaaaattc
    20761 taactttatg gtattataat aacatgaaaa tgaaatgtaa gggcttcaat ttagatgatt
    20821 atttgatatt acaacttatt ttctataaga gaaaggagtt ggtagcttga ctcttcgttc
    20881 acattctgaa cggctaatgg ggactactat cactatttca ttagtggatg agcaagccga
    20941 tatctttctc caaaaatcct ttgacttgct caaagaactt gaataccgct tcaacgccaa
    21001 tagtcaagaa tctgagttga tggaaatcaa ttatcaagca ggaatagccc ccgtcacggt
    21061 tcatccagac ctgtttgagc tgatttcact tgggttagag catagcctag cgctctctag
    21121 ccacctcaat atcagcattg gtcccttaat tcaaacctgg cgtatcggtt tttcagatgc
    21181 caaggtcgcc cagcctcaag aaattgaatc ggtgctgcct ctaatcaatc ctcatggtat
    21241 cgagttagat tcttctactt ctactgtgtt tttaaaacag aaaggaatga agatcgatct
    21301 aggttgttta gccaaaggat acagtgcgga taaggttgcc caatttctta ggaaagaggg
    21361 ggtgacttct gccttgatca atctgggagg gaatatcctg accattggaa aaaatcaggc
    21421 aagaggggat aacccatggc aaatcgggat tcaagaccca gccaatccta ggggaaatca
    21481 tttaatgacc atccctgttg tcaataaatc tgtcgtgact tcaggcattt atgaacgtca
    21541 cctgaccgtc gatggacaag attaccatca catttttgac agtcaaacag gatatcctgt
    21601 tgaaacggaa ctagcgagtc taacaatcat ctctgataaa tcagtcgatg gcgaaatctg
    21661 gacgactcga ctctttggag aaagacctgc ttctatcctc tggcaagtcg aaagtttgga
    21721 gggcatcgaa gtcatcctca tcgataaaga aggccaccta agctgttctt caggaattcc
    21781 tactctatag agacaactgt ttcaatggaa ttttaaaatt gtattatgta aaaagagaaa
    21841 ggaaatctca tgttaaaact tattgctatt gttggaacaa attcaaaacg ttctacaaac
    21901 cgtcaattgc ttcaatacat gcaaaaacac tttactgaca aagctgaaat tgaacttgtt
    21961 gaaatcaagg ccattcctgt cttcaacaaa ccagctgaca agcaagtacc tgctgaaata
    22021 ttggaaattg ctgctaaaat cgaagaggca gatggcgtta ttatcggtac tcctgagtat
    22081 gatcactcta ttccagctgt tttgatgagc gctcttgctt ggttgtctta tggtatttac
    22141 ccacttttga acaaaccaat catgattaca ggtgcttctt acggtacgct tggttcatct
    22201 cgtgcccaat tgcaacttcg tcaaatcttg aatgctcctg aaatcaaggc aaatgttctt
    22261 ccagatgaat tcttgctctc acactctctt caagcattta acccaagtgg cgacttggtt
    22321 gaccttgatg ttatcaagaa attggatgcc atctttgatg acttccgtat ctttgtaaaa
    22381 atcacagaaa aattacgtaa tgcacaagaa ttacttcgca aagatgcaga agaatttgac
    22441 tgggaaaatt tgtaagatag gagaccaaaa agaatgaaat ttgttggact tgttggatca
    22501 aactacgatc aatcatataa ccgtaagctc ttggaattta tccgtcgcta cttcaaactc
    22561 aaatttgaat tagaagttct tgaaattgac gaagttccaa tgtttaacca agatgaaaaa
    22621 tgggatgaaa gtttccaatt acgttattta tataacaaaa ttactcgtgc agatggtgtt
    22681 atcattgcta ctcctgagca taaccacact atctcagctt cactcaaatc tgtacttgaa
    22741 tggctttcat acgaagttca tccatttgaa aacaagcctg ttatgattgt gggtgcatca
    22801 tactatgacc aaggaacatc acgtgctcaa gttcaccttc gtaaaatcct tgatgctcca
    22861 ggtgttaatg cctacacgct tccaggtaac gaattccttc ttggtaaagc taaggaagct
    22921 tttgataaca acggaaatat caccaacgaa ggcactgtta aattccttga aacttgctta
    22981 gataactttg ttaaatacgt aggagtcgtt tcgaaattga aaaaaccaaa accaatcgaa
    23041 tcagaagact tggattgtgg aaaaccaatc gctacaacca tcacagaagt tgaccctgac
    23101 gatccagaat gggtagaaaa agttgcagca ttcactggtg ctgtttctgg tgatacctat
    23161 gtcaaattgg accacggtat cctaacagtt aaccaaattg atatgttctt gaaagctatg
    23221 ccatttgaat tgacatacgc tgacgacaac aaccaattcc tctactacaa caacgctcac
    23281 caagatccag acactatgtt cgctaaacgt gtaccacctc aatcaggtag ccgtatgtcg
    23341 actgttcatg gttctcttcc accagcacgt atgaagaacg tagagtgggt tatcggaaca
    23401 cttcgcaacg gaaaccaaga atacgtccgt acgatcgttc caggttctcc tgcaggtgtt
    23461 atcaacactc acaactacca agcaatgtac tatcctgatg gatcatacgc tggtatcaat
    23521 gaaatcgtct ttaacttcca accatggctt gactggtacc taaaagagac tggtcaacgt
    23581 ttggtgggtg gtagcggacc atttgctcct gctgccggag gtcatggaga cgccgatgct
    23641 acttctggcg cttctgattc aggggatgct ggaggccacg gtggtgacgc agacgctaca
    23701 gccggtgcaa gttactaatg atagttttta ggaaccatta tggttccttt tttagcgaaa
    23761 aaagactagc aaacacaaac tgctagtctc tttgttatcg tttcatctat ctttgttaaa
    23821 agaaagaata catatactca atgaaaatca aagagcaaac taggaagcta gccgcaggct
    23881 gtacttaagt acggcaaggt gaagctgacg tggtttgaat ttgattttcg aagagtatta
    23941 ttatgaattt ggatcatcaa gactacggcc atgtaatcct ttttcacgtt gtacttggcg
    24001 tagtttttct ggtgtaacat cgtttccttc ttcgtccaca attttgattc cttcaatgtg
    24061 gtgacgaaca gcgcggcgat aaccttcgat gtactcctca cgtagtttgg cttgttccac
    24121 tttttcttct ggtgttaagc cttctgtttt tttcttttta gcaagctcat tgatacgagc
    24181 aatttttttc ggatccattt cttctccttt gtaataagat aaagtccgtt tatcaaattt
    24241 tatttagtgt taatttggtt aaaacaagca aacttagctg ctttcttggt tagttgtgac
    24301 aagattgcca aacgattttg acggacagcc tgatcttcag ccattaccat agtattttca
    24361 aagaaagcat caatgactgg gctaagcgca aagagttgtt tcaattgctg acttgcaggt
    24421 cctgataaaa cgagtgtttc tactgcttct gccaaagctt tttcttggtc attctcaaat
    24481 agtgctgaat caactgtagc aaccccttct gccttctcag ccaggttaaa ggcacgagaa
    24541 agtgattcaa cagatggttt aaaatcttct tccttgctta cttctacgag agcacttgct
    24601 gcttccaaca tatctgccac aacaaagttt gaacctgcaa gaactgcttc cttgatatct
    24661 tttggagtag agcccatcat cttatcaaca cgagccttga taaagtccat aacctctgct
    24721 ttattttcat aagtcaaact gtcaaatttc aatgcataaa ggctatcaat cagctcatcc
    24781 atagcaatgt gccaaccaaa ggcatccaag atacgaacca caccttgagt tgcacgacga
    24841 agggcataag ggtcattaga acctgatgga atcaatccta ctgagaagaa actcaaaatc
    24901 gtatccaatt tgtctgcaat ggctagaact gcgccgacct tgctctctgg aagttctcct
    24961 tcagctgatg taggcatgta gtgttcacga atagcagctg ccaccgctgg agtttcacca
    25021 gcaagaaggg tgtatttttc acccataatt ccttggagtt cgtcaaattc accaaccata
    25081 cctgtcaaca agtcaaactt gtaaatggct gctgcacgag caaggtcaac tgtttcatcc
    25141 actgacaaac tagctttttc tgccaaaagt acagtgattt gacccgtacg aatcatgtgt
    25201 tcacgaaggg aaccaatctt ctcatggaag gtgacattgt ttaatttttc aacaagatct
    25261 gaaatcacca atttttggtc ttcacgccag aagaattctc cgtcttccaa gcgggctacc
    25321 aagacttttt catttccttt gatgacattt ttcaaacgct ctgcgtttcc gttacgaaca
    25381 gaaatgaagt ttggcaagag ttttccatct tgatcacgaa caacaaagta acgctggtgt
    25441 tccttcatag aagtcaccaa gacttcttct ggaacttcaa ggtatttagc atcaaaactt
    25501 cccatgaagg cagttgggta ttcaaccaaa ttcaagactt cattcagcaa atccgcatca
    25561 atttcgatac gtacaccatg ttttgcctca atttccttga tttggtcaac aatcatttgc
    25621 tcacgttcac atggatctgc gattacaaac tgcttacgaa ggtcttcttc atagctcaat
    25681 gctgactgaa ttttggtttc tttccccaag aaacgatggc cacggctcac acgacttccc
    25741 ttgatatcaa ggaaatccaa gtcaaactct tgctcatcca agagaacagt taaagtgtga
    25801 acagggcgga tgtattcaaa gctatttcca gcccagtgca tgctgacagg gaaagtcaat
    25861 gacttcaaga catctacaac accaggaaca atggcttcaa ctgcttgacc aacttcttcc
    25921 ttagtgacat agacatattc ttcacccttg atttcacgga attcgatatc ttcaacagtc
    25981 aagccttttc cacggacaaa tccttgagct gctttggtga agtttccatc actatccaag
    26041 gcaattttct ttgctggtcc cttgaaatct tctgtcaaat cagactgttt gtctgataaa
    26101 ccagtcacac gaacagccaa acgacgtggt gttgagaagg tttgaatggc ttcaaaagac
    26161 aggcggtttt ccttgaggaa ggctgccatt ttttcgccta gttgtttttc acttggtgtg
    26221 acaacatagg ctggtaattc ttcaagaccg agttctacta ataagttttt tgtcatgttt
    26281 tttctccgaa aagtatcttt ttattttcca gtttgcctta tgtgattggc acgcaagcaa
    26341 ccaacttcgt tgtttcattg ctaaaattgg agccaaaagt ctccaatttt gcctagtatc
    26401 cttagtttca ctaaggatac cttcatcact gcggcaactg gctcagggct cactctgttc
    26461 gcccattttc gtaatttgtc actctcttta ttctgcgtct tctgctagga gtttagctct
    26521 tgtttcttca tccaaaagtg ggtagcctag gcgtttgcgt tctgcgacaa aggttttggc
    26581 tacgacacgg gccaagttac ggatacgagc gatatagcct gcacgctctg ttacagatac
    26641 ggcaccacgc gcgtcaagca gattaaaggt atgtgaacat ttgagaacat agtcataggc
    26701 agggtgtacc aagccttctt ctaatgcacg accagcttct ttttcaaact tatcaaagtt
    26761 ttcaagcaac atttcttggt tcgaaatttc aaatgaatat tttgagtgct catactcagg
    26821 ctggataaag atttctccgt attttacacc atcagcccac tcgatatcat agacagagtc
    26881 tacttcttga atgtaagaag ccaagcgctc caaaccatag gtaacttccg cagtcacagg
    26941 gccagttgcc aatccaccga cttgttggaa ataagtgaac tgagtgattt ccattccgtc
    27001 aagccaaact tcccaaccaa gaccagctga accagttgat gggttttccc agttgtcctc
    27061 aacaaaacga atatcgtgct ccaaaggatt gattcccaat ttttccaaag actcaaggta
    27121 aagttcttgg atatttgatg gagaaggctt catgaccacc tggaattggt ggtgttggta
    27181 gagacggtta gggttttccc cataacgacc gtcagcagga cgacgtgatg gctctacata
    27241 agctgcattc catggctcag gtccgatagc acgaaggaaa gtgtaaggac tcattgttcc
    27301 cgcacctttt tcattatcat aagcctgcat aagcatacaa ccttggtcat tccaaaattg
    27361 ttgcaaagtc aaaataattt cttgaaatgt taatttctta gacattgaat actccttaat
    27421 aaaaaataat ttcttgcgac acgagtctgc ctcgtcgatt atacgaggca agacgagtta
    27481 gtactgcgtc tagctcaggc actctagtgc tgagcgtgga gcagcccgac tgtaaggagg
    27541 tctctgccac tgatgcagtg aaatgttaat ttcttagaca ttgtttactc ctttagtttt
    27601 tatatttctt tttcaattaa tttctgctga acaaaccaat ctaacaaggg aaaagttttt
    27661 tctgtatttg cccatagatg ataagaatca aagaccgctt gtacactacc taaattttct
    27721 accaacttat ctactatcaa aaaactgagc caatattcat aaaaaatact tgcatagttc
    27781 ttatcataag tcgaagaacc aaaatcattc aatgaatgcc aaccatactt cttctgaaaa
    27841 agttctacta gagattgatt acaaattttt tccgcttgaa attcctcttc tgtcaagaaa
    27901 tacttgcgac taatatattc aaccatcccc tcttcgaacc agatataaga gtcataacca
    27961 tcaaaatcat ctaaaaaatg ctccgaccaa tgagctaact catgtcctac aatctgtaag
    28021 aggaaatttt cagataaaga atgatagtga ctttctattg cttgagtttg ctgagaacac
    28081 tcgtagtcca tcaattgata caaatacaac tctttccaaa cagctaaatc aggcgtcata
    28141 accattcgtc tattatttgt ataggctgga acagcgcttt ccctaataat ctgtgtagca
    28201 gcattaaaat ttgaccaaat aacggcttgc ggtaaatcac agactgcaag ttcattcttt
    28261 aaaaaagatt gataatcttt caacttttca gtatttctta ctacaaaatc acgaaatgca
    28321 gctagttgac tatcgtcttt tacaagataa aggttttcca cgtctctcct ttctttcaca
    28381 caatagactg aataagcaaa agaaccacat ctgacaatcc taggttgatt taacctaggg
    28441 gcgtcagaaa cgcggttcca ccctaattta ttacttcatt gtttttgaaa atactacaga
    28501 aagcgccagc tcattatttt gccctacttg gctcccactg tcccaagctc gcttgaagaa
    28561 cgccataaga ctttctttcc tgctgactat tatatcaaaa attagaataa tgtacaattc
    28621 tttttatgat tttctagaaa tccacatcat caactcgtgg agcacccgat tgaacagcaa
    28681 tcgtttttaa ggtttctctc tcctcatgac tcagttcaat tccaaaacaa tcaagattag
    28741 cttgaatacg agaggttgtg acagattttg gaagtggtaa aaatccttct gccaagctcc
    28801 aggccaaggc tatctgagca accgattttc cgtgatttgc tgctatttct tggacttgct
    28861 tgctatcaaa cagttctcct tgtccaaaag gcccccaagc ttccaataaa attccctttt
    28921 cacgacagta agctacgact tgatcttgat acacacctgg cgccaagcga acttgattga
    28981 ccgcaggaac gatagttgca gtttcaagca aggcatccaa atgatgggga agaaaattgc
    29041 taacgccgat agcacggatt ttcccttctt gatagaggtc ttccatcgct ctccaaactt
    29101 ccgcattgcg agttttccat gcgtcatttt ctctgagcgg ttttgggttc ggccaatgaa
    29161 tcaaatacaa atccaaataa tccaagccca gtttttctat agatttttcc aaagcttgac
    29221 gagtttgctc ataggtttgc tgactattcc aaagcttggt agttacgaac atttcttcac
    29281 gtggaactcc gctatctttg attgcttgac caacactttc ttcattctga taaatcgccg
    29341 ccgtatcaat atgacgataa ccagccttca aggcttctaa cactgcacga taggcttctt
    29401 ctccatcctt agccttaaaa gttccaaatc ccaatactgg aatttctact ccattattta
    29461 attgataggt attcatatgc ttctccttct attttattca tcaattatag cataaaacat
    29521 tttttaagta gtactatctt ctgtaaaaaa taagtctgtc aaatatctaa cagacctatt
    29581 ttttcttgct tattcatcac gactatagta cagctcatga gcttttaggc gagtattgag
    29641 catctctggt atggtcacaa aggtataacc ttgatttttc aaatactcaa tgacccttgg
    29701 caaggcattg actgtcggac tgtgaatatc atgcatcaaa acgatagagc cgttagctac
    29761 ttgatgctga atttctgtca aaatagatgc ttcattttta ctcttccagt ccagactatc
    29821 cacatcccac atgataaagc tcaaatccaa gctattgcga atatcatctg taatagcacc
    29881 ataaggtgga cgcatgagtt tagaactaga acccagcact ttagttagca catcctcagt
    29941 atcagtaatc tgctttttag cttcatcaag agagagttgc gagagaatcg gatggctcca
    30001 actatggttt ccaacaacat gaccttcaga ttttatcctc ttcaccaagt cctcattccc
    30061 agaaacattt ttcccaagca caaagaaagt cgctttaata tcatatttag ctagggtctc
    30121 taatacctgc ggggtcgttg ctggatttgg accatcatca aaggttagag cgacaacttt
    30181 ttgatgtttc ttatcaaagt aagattggta caaggccgca tctttttcga gtaagtacga
    30241 agattggata acatcaaaga aagcagatac tggcaaggct atctcttcta aattttcaac
    30301 cacaggactt ggataaagga taatctgact atccttgtaa tcaaaattcc atgcagacaa
    30361 gtcttggtca gagaagtttt ttacaatctg ctcactctgg tcttgctcta tttttttatc
    30421 ctctatgaag gaggtcaact cttttatcag ctgttcctta gccttactag catctgaaaa
    30481 cagttggtca agtgtaaaag gttgcccatc ttctgttaag tgaacctttc ctagactggt
    30541 cttttcagtc tcttcaatct taaaagcaac taaatcatag atttgtttgg tcacattacg
    30601 attaacgact ccctttaaac ctgactcttc ttgttctgta tagtagaaaa caagattgtc
    30661 cttactttct agcttgtcct taacatcttg atttatcagc tccctaacag aggaaatcac
    30721 tttctcccct tggagaggat aataggcaat cacttcggct tgcccctgac gaaaatgctc
    30781 cttctgattt ccctcactca attgatcatc tttctctttt ttgagcgatt caatcttttg
    30841 ctcaaaactt ttttgctggt agatcttaaa agcaataaat ccgcctaata ggcaaataga
    30901 aattgctaaa ataccaatca aagccaataa tacatgtctc gttttcccgt gtctgccacg
    30961 tcctagtcta cttttattca tatttatatc ataacaaatt tgagcaataa tttcaatgat
    31021 acaatttggc aatccacaat tcctctagca agccaaagca tcttacatca actccaacat
    31081 ccgtatctgt agagattatg tagttttcta tctatggaat ttctagtaga ttgaagctag
    31141 aatagtagac ctatacttct aaaacattgt tagaaatcga tttgactgtc ctgatcgatt
    31201 tgtcctgttc tcatttcatt tcactataaa actatgaaaa gctaggtcat ctgagaagta
    31261 attactatcg acaattgcga tagcgtatac tagtgcgatt tgtttgtagc tctggtgggt
    31321 atcaccttca cgttggcgaa ttttttctag gttttgatta acctgaatgc acagataagc
    31381 ccataggcga ttgatgaaaa aattctgatg atgaacctga atttcgatga taacttggat
    31441 tccattatct aactccgcca agacgtctat actagtatag aaatcttgcg ctgagtaggg
    31501 catggaaggc aagacgtgaa tgttacttcc ctccaaaatc gtcacatttt ttgctggcaa
    31561 gtccagcata tcgcggataa attgacaaat cattagttgg gctgatacct ggatgtctga
    31621 gaatcctcct tttcctcctt ctattatagc acatttttaa atctaggttt gctagattct
    31681 atgcttctat ctatttattc ggaaaagaaa gctaatagct tatttgaaat tttctcctct
    31741 ctgttggata gtcccgtcta tctatgttat aatgaaagaa ggatttaaaa tcggaaaagg
    31801 agtatttatg ctaaaattag gtgtcatcgg aactggcgct atcagccatc atttcataga
    31861 agcagcccat accagtggag aataccaact ggtcgcaatc tattctagaa aactagaaac
    31921 tgcagcaacc tttgcttctc gctatcagaa tatccaacta ttcgatcaat tagaagtctt
    31981 cttcaaatct tcttttgatt tagtctatat cgccagtcca aactccttac attttgctca
    32041 ggcaaaagct gccttgtctg ctggtaaaca tgttattctc gaaaagccag ctgtcactca
    32101 accacaagaa tggtttgatt tgattcaaac agctgaaaaa aataactgtt ttatctttga
    32161 agcagctcgt aattaccacg agaaagcctt tactactatc aaaaactttt tagcagataa
    32221 gcaagtgtta ggagcagatt tcaactatgc caaatactct tccaagatgc ctgacttgtt
    32281 ggctggacaa acaccaaatg tcttttcaga ccgttttgct agtggagccc tcatggattt
    32341 ggggatttat cccctctatg ctgctgttcg tctttttgaa aaagctaatg acgcgactta
    32401 ccatgctcaa cagcttgaca atagcattga cctaaatgga gatggtatcc tcttctaccc
    32461 agactatcaa gttcacatca aggctggaaa aaacatcact tccaatcttc cttgcgagat
    32521 ttatacaaca gatggaacct tgactctcaa cacgattgag catattcgct cagctatttt
    32581 taccgaccac caaggaaatc aagtccagct tcctatccaa caggctcctc atacgatgac
    32641 tgaggaagtc gctgcatttg cacacatgat tcagcaacca gatctgaatc tctaccaaac
    32701 ttggctgtat gatgcaggct ctgttcatga gctactatat accatgcgcc agactgctgg
    32761 cattagattt gaggcagaaa aatgaaaacc aaactaccga ctgaatggca agaactgagt
    32821 gaccaactcg gtttccaaga attcaccccc attcaaactc aactatttga gccccttctt
    32881 gctggagaaa acctcctagg agtgagccaa accggaactg gtaagaccct agcgtatctc
    32941 ctaccaagtc ttctcagact acaaaagaaa aaagcccaac aactcttgat tctagcacca
    33001 aacacggaac ttgctggaca gatttttgat gtatgtaaaa cgtgggcaga agctatcgga
    33061 ttgactgctc aactcttcct ttcaggttca agtcagaaac gacaaattga acgtcttaaa
    33121 aaggggccag aaattctgat tggaactcct ggccgtatct ttgaactcat taaacttaaa
    33181 aaaatcaaga tgatgaatgt ggaaaccatc atcctggatg aatttgacca attactagat
    33241 gattctcaga ttcactttgt agaaaaaatc actcactacg cacctcgtga ccaccaactg
    33301 gtctacatga gtgccacgac caagtttgac caagaaaaga ttgtgccgaa tacacgcact
    33361 attgatctct ctgatcaaaa attggacaat atccagcatt tctacatgca ggtagaccaa
    33421 cgtcatcgag tggatatgct acgaaaactg gctcatgtag aggatttccg tggtctagtc
    33481 ttcttcaata gcctgtcaga ccttgggaat gccgaggaaa aactacagta tcgtgatata
    33541 ttggctgttt ccctcgctag tgatgttaat gttaaattta gaaaaatcat cttagaaaag
    33601 tttaaagata accaactaac cctgcttctt gcaactgacc ttttggctcg tggaattgat
    33661 atcgatagtc tagaatgtgt cgtaaacttt gatattccta gagatagcga aacctacaca
    33721 caccgtgctg gccgtactgg tcgtatgggc aaggaaggtt atgttatcac tctcgtcact
    33781 catccagaag aaatcaaaaa acttaaaaag tttgcaagta tccgagaaat tgtcctaaaa
    33841 aaccaagaac tctatatcaa ataaagcccc attgctctat acacatccag tgtatggagc
    33901 ttttttataa gcaattcaat tttatactca tctgttttca aaaagcattc tagtccatct
    33961 ccgattaacg atggacttta tcacctcctt ctccaatcct tgtatgacat cttg
//
LOCUS       DAVKJJ010000021        30957 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000021 DAVKJJ010000000
VERSION     DAVKJJ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30957
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.021"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(<1..1532)
                     /locus_tag="VW144_000981"
     CDS             complement(<1..1532)
                     /locus_tag="VW144_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEU9600443.1"
                     /translation="MLYLLNKDVRTVRWNGEPLHEATSAIVKEIMNGDFTLTVKYPIS
                     DSGIYQLIQEDMLIKAPTPVLGAQLFRIKKPVEHNDHLEITAYHISDDVMQRSITQMS
                     VTSQSCGMALSRMVQNTKTALGDFSFNSDIQDRRTFNTTETETLYSVLLDGKHSIVGT
                     WEGELVRDNFAMTVKKSRGENRGVVITTHKNLKNYQRTKNSQNVVTRIHAKSTFKPEG
                     AEKETTIRVTVDSPLINSYPYINEKEYENNNAKSVEELQKWAQAKFSNEGIDKISDAI
                     KIEAYELDGQVVHMGDTVNLKSWKHNVDVFKKAIAYEFDALKEEYISLILDDKAGAGG
                     SRTSGGLSSAADAILGVTESAQEVALEKALQNADLDFDHKAGLLRQEISDGIELAKAK
                     AEEVKQELSDTINQRFNSFDNGPLKEAKRRAEEALRNAGASSLLAQEAKRIGLDSVAR
                     LEEFKSQTTSAQTALSGDLDALKRTIVNDIRPKQAQVEAEIAKQVEALVQTKKELSGA
                     STL"
     gene            complement(1533..2255)
                     /locus_tag="VW144_000982"
     CDS             complement(1533..2255)
                     /locus_tag="VW144_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEU9600444.1"
                     /translation="MIKHNELVIDGVRTSSFPFKVIVHDSPSIALGESKTALLEHGGI
                     SGAIVQTNKHRELVKKSYTIYLVKPTEEQMNQFMSLFIREKFWLESERVKTTRLWCYK
                     VNVTDLEEVQPGLYMTKATFTCHPTKYFKATDTQRLTRSGTLNVQGSALAFPKITIVG
                     QSVVETSFTIAGQVIRLEKLSESLVMVNNPDNPSFKTTTGKPVKWSGDFITVDPAKVK
                     NIGVVLGPGIQSIEIETVWGWA"
     gene            complement(2252..5011)
                     /locus_tag="VW144_000983"
     CDS             complement(2252..5011)
                     /locus_tag="VW144_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEU9600445.1"
                     /translation="MAGNIKGIKIEIDGDTQPLQKALKNVNKAATDASQELRQIDKAL
                     KFDTGNVTLLTQKQEVLQKQVSTTKEKLETLRQAQSQVEQQFKNGDIGADQYRAFQRE
                     VEVTQNVLKGYEGKLANVNQALNENGSATQNNKNQLKELQNEQSQLASEMSKVTSSFK
                     LQESALGSNASEAERNALAQKKIGAQSEIVSKQISNLEQQLEITKKEFGENSTQANRM
                     EAELNQAKTAFNHLNDEMKGTKSAADSTQESLSEISRNLRAELLQQFSEKLSAISEKL
                     VEVGKEALEAAAQMQASNAQFTTVFGDMETQAREALNAIGQEMDIVPERLQGSFTQMA
                     SFAKTSGLDTAEALDLTSRATRAAADGAAFYDKSIESVTESLQSFLKGNFANDAALGI
                     SATETTRNAAANKLYGKSFKDLSEAQKQLTLLQMVEDGNKLSGALGQAARESDGLENV
                     MGNLKQAGTNALSAIGQPLLEMMIPVFQTLATIVKGVAELFSSLPAPVKDFVVILGTV
                     VTAVGVIAPIFLSLQALAEFLKISIGEMIIAALPIIGTAIAIAAAVAAIIVIVKYLWE
                     TNEGFRDAVTTVWNAILEVINAVVSEISNFVMSIFGTVVAWWTENQELIRASAETVWN
                     AIYTVISTILEILGPLIQAGWDNIQLVITTAWEIIKTVVETAINVVLGIIQAVMQIIN
                     GDWSGAWETIKGVFSTVWQAIQSIVQTIFSAIQSYISNILNGISGTVSNIWNSIKDTV
                     SNVLNAISSTVSSVWEGIKSTISSAINGARDAVSSAIEAIKGLFNFNISWPHIPLPHF
                     YVSGSANPLDWLSQGVPSIGIEWYAKGGIMTKPTIFGMNGNNIMVGGEAGNEAVLPLN
                     DKTLGAIGRGIAQTMGGTSPTINITITGNTVREEADISRIADEVAQRIADELQRKTQL
                     RAGVT"
     gene            complement(5289..5708)
                     /locus_tag="VW144_000984"
     CDS             complement(5289..5708)
                     /locus_tag="VW144_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600446.1"
                     /translation="MRKIVLVGDQEYELGTNGYTPIAYKQQFGKDYFQDLFSMLKNQS
                     FMNELNKLETDKELTATNIDISMLSDFDMTFFNRLFWTFAKSENPHIKPYEQFFMEME
                     VFPIQEVGPVLMEMLNASMTTKKHQMNQNQLAKKSSQ"
     gene            complement(5720..6298)
                     /locus_tag="VW144_000985"
     CDS             complement(5720..6298)
                     /locus_tag="VW144_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEU9600447.1"
                     /translation="MTQENKVTFGLENVHIAPIKTLAADGVITYGDVFRFPGAIDLTL
                     DTKGETTTIKADNKDYHFMNSNEGYEGKLKIPHIIDEFATKILGEIKDPQTGVMTEKA
                     DASLTEFAMMFQFEGDKNKTRYVMYYCFASRPSLGSKTKNGTSTNERELSFKASPRPL
                     DTVVKRSITSADDKDAYDNWFKKVYEPTAVAA"
     gene            complement(6310..6633)
                     /locus_tag="VW144_000986"
     CDS             complement(6310..6633)
                     /locus_tag="VW144_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600448.1"
                     /translation="MKLSDFAAILEQANLPVTYRAFKIGNAPNLPYLVYYESIPAINA
                     ADNTVNHQIKSVTVELAFESKDEDLEERLEELWANHKLFFEVQEETFIETERLYVKSY
                     TVYLY"
     gene            complement(6630..6989)
                     /locus_tag="VW144_000987"
     CDS             complement(6630..6989)
                     /locus_tag="VW144_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEU9600449.1"
                     /translation="MSLTSDLANEIAKAMAEYSAEVEDKIDLIAEDVVNEAVTELKAT
                     SPKRHGKYARNWRFKKNAKGSYVIYNAAPTYRLTHLLENGHVLRNGGRSRAFPHIKPV
                     EEKVKENFEKRIKEIGK"
     gene            complement(6986..7285)
                     /locus_tag="VW144_000988"
     CDS             complement(6986..7285)
                     /locus_tag="VW144_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEU9600450.1"
                     /translation="MWNHEITLISKEVTGKDKLLQPISEDVEVTLLCRKKRVTRSEFY
                     QANQVGLKPSLVVEIRNFEYENQELAKFEGKQYHILKTYPIDSEILELTLSEVLK"
     gene            complement(7272..7553)
                     /locus_tag="VW144_000989"
     CDS             complement(7272..7553)
                     /locus_tag="VW144_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000371962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600451.1"
                     /translation="MDNVQLLELLKLKLGIATKLRDKPLEKIIEAVKTELEDNLGVLL
                     DLDSSEDQMFVVDFAAFRYEGGVDMPRHLQWRLHNLQIASKKEVKNVES"
     gene            complement(7556..7825)
                     /locus_tag="VW144_000990"
     CDS             complement(7556..7825)
                     /locus_tag="VW144_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600452.1"
                     /translation="MVYKVIRPFKDLRDPKKHCYKVGDNYPRVKHKLDKEFAETLLNG
                     ANSAGSIFLMNVEDKDMSKDDTLTTEVEKEETKVVDGKPEEGDEE"
     gene            complement(7837..9003)
                     /locus_tag="VW144_000991"
     CDS             complement(7837..9003)
                     /locus_tag="VW144_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEU9600453.1"
                     /translation="MNKALIFGARMRAKATKVVELEETIEELNKRSVVELEKLDRAKN
                     DEEVLAVEKTVDGLQREIEEKEAEKVQLENEIDELDKQIKEQNRKAPTPGKMEERGGK
                     TLGQREAFNHYLRTKEARADGFKSAEGEAIIPVELMTPKEAKQDKTDLTSLVNIVNVK
                     NASGKWSVVKLTDQAMNTVEELEENPELAKPTFTKVNYEIKTRRGHLPVSQEFIDDAD
                     YDVMGLVAKQTKNQERITKNKEIAKVLKTATSKSAAGLDGLKDILNVELKTYYNATIV
                     CTQSMFAALDKIKDKDGRYMLQTDITSPTGYKFAGRVIVVYPDDIIGESKGDLKAFIG
                     DVGEFATLFDRAQTTVKWQDDKIYGQYLATANRFDVVKVDGDAGFYVTYTDAVL"
     gene            complement(9000..9575)
                     /locus_tag="VW144_000992"
     CDS             complement(9000..9575)
                     /locus_tag="VW144_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEU9600454.1"
                     /translation="MQKRNSYRATQFQTREEESGDLVLSGYFIKFDEETELWRGYHEV
                     IKRAGVEKAVTDADIRALFNHDDSLVLGRTGNGTLTLGVDDVGLFGDIIINKDDPQAV
                     GAYARVKRGDVIGCSFGFIPVKIETEEREDGSYLDTVLELEIFEVSPCTFPAYPQTEI
                     AARQKDFESQKRANREALDKRKKEIKEKFKL"
     gene            complement(9559..10761)
                     /locus_tag="VW144_000993"
     CDS             complement(9559..10761)
                     /locus_tag="VW144_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEU9600455.1"
                     /translation="MGIFEKFWKRNKPSKSINMLSHSDLGLSNLMDSYVPLARNPDVV
                     TAVNKIADLVSNMTIHLMENTDKGDIRIRDGLARKIDINPCKHMTRKSWIFKIVRDLL
                     LYGDGNSVLHVEYEPVTDYISNLRPFPMREVSFQTDKDSYVISFRGEEYSPDEVVHFV
                     INPDPDILYIGTGFRVTLTDVVQSLNMATKTKKSFMNGKNIPSLIVKVDSSSAELDSE
                     QGRERIAEKYLSTSRVGAPWIVPEALLDIQQVKPLSLTDIALNESVELDKRTVAGLLG
                     VPAFILGVGEFNKTEYNNFVNTTVMSIATTITQTLTRDLLLSSNRYFKLNPRSLFSYN
                     ITELSAVAQQMANSAAMRRNEWRDWLGMAPDPEMEELIVLENFLPQEKLGDQNKLKGG
                     EEENAKAK"
     gene            complement(10779..10997)
                     /locus_tag="VW144_000994"
     CDS             complement(10779..10997)
                     /locus_tag="VW144_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600456.1"
                     /translation="MNEIVLSEHEINLLINKGRVKVILNGEVVTIRQRHMKNLMAETV
                     KWEKQVIDVSQNIVRNKHFDSLFQNTFR"
     gene            complement(11005..12735)
                     /locus_tag="VW144_000995"
     CDS             complement(11005..12735)
                     /locus_tag="VW144_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEU9600457.1"
                     /translation="MGNLDKAKKYAQHVLTHREEHCEENILAAERFFRDLENPTFEMD
                     EDMVDFVIHFIENVIVHQQGDDMFAVSIRNKPLLLQPWQHFVVVNLFGFYYKGTNERR
                     FKEALIMLARKNGKTSFTAAIALAYQILDTDSGSKCYIVANSVKQAMEAFGFLRFNVE
                     RWNDKNIRIKDNNQEHSITANFGDEGSFFIQALANDESRLDSLNGNVIILDEAHTMRN
                     SKKHGLMKKTMSAYRNSMLFVISTAGDIPTGFLANRLKYCQKVLKQLVTDDSFFIFIC
                     KANQSADGDVVNYLDENILKMANPSWGVTVSLKALKEEAEQAMNDPQTRNEFFNKTLN
                     IFTNSMNAYFNPDEFIASDSCYDWSLEELARLPIRWYGGADLSRLHDLTAAALYGVYH
                     DGEKDVDICITHAFFPRINAQKKANDDGIPLFGWQSDGWLTMSNTPTVLYDDIVKWFI
                     KMREKGFKIAAVGMDRKFGREFLTKMKQARFKMIDQPQLFYLKSEGFRRIEFKVKNKE
                     FYYLHSDAYEYCVSNVRAIEKVDDAVQYEKLDGDGGTARIDLFDASVFACIQALANLG
                     KGGDVMRFFD"
     gene            complement(12728..13120)
                     /locus_tag="VW144_000996"
     CDS             complement(12728..13120)
                     /locus_tag="VW144_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HEU9600458.1"
                     /translation="MAKPITAKSIKSKVVKQMKDLGTYRKEFEMIIDIFAGMLYQYQK
                     LAQDYVDMGYPVTDTYVNKAGAENERKVPILTAMEILRKDILSYSNQLMMNPKSLGEV
                     VEQEGESVLTEVLKFKNEIKKKRVTGNG"
     gene            complement(13257..13577)
                     /locus_tag="VW144_000997"
     CDS             complement(13257..13577)
                     /locus_tag="VW144_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEU9600459.1"
                     /translation="MYNKPIRPSLKSKKWEKFRDRIMRKHDYLCQESLRYGISVQAEM
                     VHHIFPVSEYPELEFVEWNCLPLTNKKHNTFHDRVNDRVINQGLYWQKKRKKEFLNFF
                     KNEK"
     gene            complement(13707..14108)
                     /locus_tag="VW144_000998"
     CDS             complement(13707..14108)
                     /locus_tag="VW144_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9600460.1"
                     /translation="MKKLSDEDLKTLDRELFKFQNIQRTIDLRRLELETRNPDAQSGP
                     SVGISKPTETIAVRIADDPTLKFLEGFKAIINKLLINLVDEDKEIFNLRWRYPQLRWE
                     EIAEQKFMSKATIYRRRRIILEQYAILKGEL"
     gene            complement(14245..14655)
                     /locus_tag="VW144_000999"
     CDS             complement(14245..14655)
                     /locus_tag="VW144_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600461.1"
                     /translation="MIPKFRGLSIDEKSKGEWQYGHLIEDRGRAFIINEVVEANEQYI
                     TIGSWCPVNPATLGQSTGLRDKNGKEVFIGDIVKCTRGCLHEVYLEKEYGGTYIGGMP
                     AVYLKGFGDGYAWTEYEEIIGNVYENPELLEVNE"
     gene            complement(14667..14945)
                     /locus_tag="VW144_001000"
     CDS             complement(14667..14945)
                     /locus_tag="VW144_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600462.1"
                     /translation="MKRLGIIIGAVFVIVVSPFVVQYGWNEIITTIVPVGKITVWQAL
                     GMDALLSFIWPVLSSKKESEEDYSYAVKSSISKIITCAFLIWLASLFI"
     gene            complement(15047..16129)
                     /locus_tag="VW144_001001"
     CDS             complement(15047..16129)
                     /locus_tag="VW144_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEU9600463.1"
                     /translation="MKFLDLFAGIGGFRLGMESAGHKCIGFCEIDKFARESYKAIHNT
                     KGEIELHDITAVSDESIRGIGSVDIICGGFPCQAFSIAGNRRGFEDTRGTLFFEIARF
                     ASILRPKYLFLENVKGLLNHENGVTFETIISTLDELGYNVEWQVLNSKNFGVPQNRER
                     VFIIGHLRREHTRRIFPLSGKNQSTSNQSVMKIGNINPSGNGMNGEVYQADGLAPTLT
                     TNKGEGQKIAIKSNTIKQFGVLQPNFNQCGVVYETDGIAPTIRAYQGGGLEPKIIQRG
                     HGYNKGGEHNIAPTLTSNSYHENNHLSDGFRIRKLTPRECWRLQGFPDWAFDKAQEVN
                     SNSQLYKQAGNSVTVNVIAAIAKELS"
     gene            complement(16160..16447)
                     /locus_tag="VW144_001002"
     CDS             complement(16160..16447)
                     /locus_tag="VW144_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600464.1"
                     /translation="MTQTLEEGMKNQSKCIKVPREIRPFDIGYRIVNKYGQALALRNG
                     ASIFDLPFLAEKAIEKEFGKNDPDFDIGKHSVEEVAIVNLSKFHSYFEERV"
     gene            complement(16447..16977)
                     /locus_tag="VW144_001003"
     CDS             complement(16447..16977)
                     /locus_tag="VW144_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEU9600465.1"
                     /translation="MNIKALIKKYEELWNEHSPFYEPVPYTSMVELFLKELKQLDEPQ
                     KVKIPQFVADWIEVCKEHLTTSLYTAMTPNFMKENNQSFDLILWIKKASNQDLFARAW
                     LDGYEVEKEKRYLVKIKGNIKENMLVYGELLERYFFTKSFSLDDAIYSHTRKELENAK
                     IGWVFDCEGFEIEEVE"
     gene            complement(16979..17296)
                     /locus_tag="VW144_001004"
     CDS             complement(16979..17296)
                     /locus_tag="VW144_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600466.1"
                     /translation="MMEELKRKVNEVYNWTVEDGKPQPPKQDLPQAVKDRADYFWEMA
                     EDGMTFMGAMECIFADEKPTDYDLGATKGWLPKSKEFDDWVGYSPSMAQVVIAVYLIY
                     GGN"
     gene            complement(17464..17631)
                     /locus_tag="VW144_001005"
     CDS             complement(17464..17631)
                     /locus_tag="VW144_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600467.1"
                     /translation="MVGVTYQEIHLFVEFLKEQYGQGRPDYIEALNDLDGLVEVSYRE
                     AIERFLEDEVR"
     gene            complement(17716..17934)
                     /locus_tag="VW144_001006"
     CDS             complement(17716..17934)
                     /locus_tag="VW144_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600468.1"
                     /translation="MLELYFVYNGHCKFYLGRFDNVDDLIEQMEDHQWAFSAITHPRF
                     QKHIGQRTTRFDYGSKDCYYLATFSGGK"
     gene            complement(17944..18618)
                     /locus_tag="VW144_001007"
     CDS             complement(17944..18618)
                     /locus_tag="VW144_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU9600469.1"
                     /translation="MMKFLNGDCMDIMKQYPDNYFDLAIVDPPYFSGPEKRKFYGRKI
                     SPIGVSRLYGEISEWKIPNRDYFDELFRVSKNQIIWGVNYFDYSFGSGRIVWDKVNGQ
                     SSFSDCEIAYCSYHDSTRLFRYMWNGMMQGKSISEGHIQQGNKALNEVRIHPTQKPVN
                     LYLWLLQTYAKEGDKILDTHVGSASSLIACGELGFDYVGCEIDKSIFNLAQQRLDAYE
                     KQLKLF"
     gene            complement(18618..18812)
                     /locus_tag="VW144_001008"
     CDS             complement(18618..18812)
                     /locus_tag="VW144_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600470.1"
                     /translation="MFILKHGTREDKPFLRSAIISVTGLDISCSEEKKAMRFISRAAA
                     VQVGKALRGSFGNFYPVEVE"
     gene            complement(18827..19597)
                     /locus_tag="VW144_001009"
     CDS             complement(18827..19597)
                     /locus_tag="VW144_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9600471.1"
                     /translation="MVDNVFEEIALSYRRNTEQQEEFCEKHNIPLIKILRTESVVCRM
                     CESERIHEENQERVNELANAENERERKYYLEKFSLYDEVLKNATLDNFETPTEKEAEK
                     LAFAKRICREWSEGARNNIVLQGEAGTGKSHLAFAMVKALSEYTKEIAIFINVTDLLM
                     KIKADFSQEEFLVNKIASAKFLVLDDLGMEKDSEWSFTILYNILNKRSNTIITTNLIS
                     ADIQKRYGRPFMSRLMKGVDKDHLMVFNDLTNKRKQYF"
     gene            complement(19591..19749)
                     /locus_tag="VW144_001010"
     CDS             complement(19591..19749)
                     /locus_tag="VW144_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600472.1"
                     /translation="MGVLELIEQFEIDYYPLSYEKKTLLADQPIHQVVACLSEMASWH
                     ECGGRLVW"
     gene            complement(19737..20543)
                     /locus_tag="VW144_001011"
     CDS             complement(19737..20543)
                     /locus_tag="VW144_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEU9600473.1"
                     /translation="MSEIKWIKITTDIFDDEKICLIDALPDPDAILVIWFKILTLAGK
                     HNSNGLLMMTDKVHYTDEMLATIFRRPLNTVRMAIGVFEQFGMIEIIDGIISLPNWEK
                     HQNVDGMEKIKEQTRNRVAKYRKKQKNLALGNVTGNVTVTDGNALEEDKDKNKNRLDK
                     DKNKKRITTTSSGSEENILELFQSEFRRLLSGFEIEEINHLLNENDVDLVKEALKTAI
                     NSGKPNIKYIGGILRNWQMNNVTTVEQVRQSEKKNKDKKEEQEAKDEWGY"
     gene            complement(20536..20832)
                     /locus_tag="VW144_001012"
     CDS             complement(20536..20832)
                     /locus_tag="VW144_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600474.1"
                     /translation="MEELIESLDNLIMIVKELEGRESTSRHFITIWENDYKNLLLVKE
                     YLTDYEKLAKDYRYVTLKNKLLKIEKMELEGRHIYEDMRMKYRANRRKWGARYV"
     gene            complement(20848..21168)
                     /locus_tag="VW144_001013"
     CDS             complement(20848..21168)
                     /locus_tag="VW144_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600475.1"
                     /translation="MFEPPILDQLMGVGALLLGFAGACRHIKLQEQRKEEERREEQEF
                     ASMIIQGYNHAYERGREAERQEIRKNIRRPFKGFTYDNEPPQGLRPEPLALPEPKQSA
                     IRLL"
     gene            complement(21254..21511)
                     /locus_tag="VW144_001014"
     CDS             complement(21254..21511)
                     /locus_tag="VW144_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9600476.1"
                     /translation="MDNVLLSLSEWIKSIIKDTITRLVEIEKDSDHYPELMDVNTTCE
                     FLGIKYATFSDNYRYLKGFPKELPGKKWSKRAIKEWLSNQI"
     gene            complement(21525..22235)
                     /locus_tag="VW144_001015"
     CDS             complement(21525..22235)
                     /locus_tag="VW144_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEU9600477.1"
                     /translation="MNEIFNFHGQEVRTLTIDDEPWFVGKDVADILGYSKARNAITLH
                     VDEEDALKQGIPTSGGTQDMLIINESGLYSLILSSKLPQAKEFKRWVTSEVLPAIRKQ
                     GGFIREDLDEDAFIALFTGQKKLREQQATMLEDIDYLKSEQPIHPSYAQSLLKKRKAR
                     VVACLGGIDSPAYADKVFAQSVFRQAEIDFKDHFNISRYDLLPKKHADAALAYWMTWE
                     PSTNTKMKIMKLNSFDDV"
     gene            22289..22714
                     /locus_tag="VW144_001016"
     CDS             22289..22714
                     /locus_tag="VW144_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600478.1"
                     /translation="MDYQILIQPAISVILAIISGLWSYIASKANNKAEIEKQAKEHSH
                     IVEKLEKEFHYQIDTLKQQHTLELEKVKQAHELRLQELEKVSQIDTETDKAMKMNDLI
                     YKTFTGEVDLDKALKLADKANNHKQKLNKKFIQKTSKKS"
     gene            complement(22709..22870)
                     /locus_tag="VW144_001017"
     CDS             complement(22709..22870)
                     /locus_tag="VW144_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600479.1"
                     /translation="MNELVWFYFVVIINLIIGFATYYASKRDRKKRINEYKKIQDDEL
                     ERIRNKFNL"
     gene            complement(22881..23078)
                     /locus_tag="VW144_001018"
     CDS             complement(22881..23078)
                     /locus_tag="VW144_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600480.1"
                     /translation="MNNAAQKVTRIDKDAWEIATELANEYGVSICHIISESVRYCAEN
                     AEFKEMDVVVKRLVVGSKVLE"
     gene            complement(23095..23298)
                     /locus_tag="VW144_001019"
     CDS             complement(23095..23298)
                     /locus_tag="VW144_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9600481.1"
                     /translation="MSKELKIIKAKIKTRLIELDMTQAELAKQVSVTSSVISELLKYG
                     KGSDYVKEKVVDILGIENPWENS"
     gene            23353..23835
                     /locus_tag="VW144_001020"
     CDS             23353..23835
                     /locus_tag="VW144_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600482.1"
                     /translation="MKSSFFHYLKEIILYGVLVPILTVIVGFHLFTTFYPISDSLLTD
                     FLKAFTEQRILAIVFVLILCFIFFGSIYYCISCGRNRKLIPDKVYTQITTYISSVGLG
                     TAVIAVATLTLTELQFSILTGWIAFIALLLPLLKFKVQYSTYQSSDNVAQKTTENKAN
                     "
     gene            24141..24509
                     /locus_tag="VW144_001021"
     CDS             24141..24509
                     /locus_tag="VW144_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9600483.1"
                     /translation="MFVAFDKIKELADKQGISINILEEKLGYGTNTLYRLKRSNPSSK
                     VLKEIADYFNVSADYLLGRTDNPAIAGSDEFAQVNGQIIDLRKAAANTMLFDGKPLNE
                     DDIDFITSVLSAHFKSKGER"
     gene            24509..24742
                     /locus_tag="VW144_001022"
     CDS             24509..24742
                     /locus_tag="VW144_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600484.1"
                     /translation="MTITINFTEKNSYITDYLTKHGIDTTTMDFDDFMVLMEDIEDAR
                     AADQAYMEYLADPATYTMDEVLDELGLSREDIA"
     gene            24742..25005
                     /locus_tag="VW144_001023"
     CDS             24742..25005
                     /locus_tag="VW144_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU9600485.1"
                     /translation="MYRLDIDKKALKQLKKLDTPTRKQILSWLAKNIENTTNPRQHGK
                     ALKANLAGYWRYRVENYRIICDIQDDKLVVLAVEIAHRRDVYK"
     gene            25018..25398
                     /locus_tag="VW144_001024"
     CDS             25018..25398
                     /locus_tag="VW144_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEU9600486.1"
                     /translation="MTLAKLCEEYQVELCLFDGSNWHSSGFYNPDTNVLAIDHNLAPE
                     QQIQVALHELGHKDHTRSEYQNARLRCENEADRNMIHHLVKDALESLDDPTEFDYLKF
                     MSYYNLKTMTNEVMVKEEYLALVN"
     gene            25436..26248
                     /locus_tag="VW144_001025"
     CDS             25436..26248
                     /locus_tag="VW144_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEU9600487.1"
                     /translation="MQKTVEKILFRVAGVTKYKKAVKEACNMIAEDNGIPEYAKYYSN
                     LSTKELREELEEYGLKVFKYQDLDFFNIELVPEVDNRYDPNAIKVLIFDNHVGYVPAT
                     IAKTIRKYFDNKKYNFVIEGEIKGGPYKEWDEYEEKVVTNNDLDVGFEIYLTIVDSSQ
                     KEVIQSESSEIIDDNISNKEVTETEAIETKTTETEHIEQNIVSDSVADIVNEINLSET
                     SPKKKLPANKIIFSALYLFLVFFGVVGIPIAPFLAVPLTAWSLYKLYKLFRK"
     gene            26422..27570
                     /locus_tag="VW144_001026"
     CDS             26422..27570
                     /locus_tag="VW144_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU9600488.1"
                     /translation="MKYNKTKYPNIYYYETAKGKRYYVRRSFFFRGKKREKSKSGFTT
                     LPQARAALVELEQQIQEQELGINTNLTLDQYWDIYSEKRLSTGRWNDTSYYLNDNLYK
                     NHIKAKFGSILLKNLDRNEYELFIAEKLQNHTRYTVQTLNSSFMALLNDAVKNGNLLS
                     NRLKGVFIGQSDIPAANKKVTLKEFKTWIAKAEEIMPKQFYALTYLTIFGLRRGEVFG
                     LRPMDITQNDSGRAILHLRDSRSNQTLKGKGGLKTKDSERYVCLDDIGTDLIYYLIAE
                     ASKIKRKLGIIKEQHKDYITINEKGGLINPNQLNRNFNLVNEATGLHVTPHMMRHFFT
                     TQSIIAGVPLEQLSQALGHTKVYMTDRYNQVEDELAEATTDLFLSHIR"
     gene            complement(27812..29098)
                     /locus_tag="VW144_001027"
     CDS             complement(27812..29098)
                     /locus_tag="VW144_001027"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU9600489.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(29329..29565)
                     /locus_tag="VW144_001028"
     CDS             complement(29329..29565)
                     /locus_tag="VW144_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEU9600490.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            29830..30603
                     /locus_tag="VW144_001029"
                     /pseudo
     CDS             29830..30603
                     /locus_tag="VW144_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(30722..30795)
                     /locus_tag="VW144_001030"
     tRNA            complement(30722..30795)
                     /locus_tag="VW144_001030"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30759..30761),aa:Asn,seq:gtt)
     gene            complement(30801..30916)
                     /gene="rrf"
                     /locus_tag="VW144_001031"
     rRNA            complement(30801..30916)
                     /gene="rrf"
                     /locus_tag="VW144_001031"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         9047 a         7011 c         5139 g         9760 t
ORIGIN      
        1 agggtacttg cgccagacag ttcttttttg gtctgaacaa gtgcttcaac ttgcttggca
       61 atctcagctt caacctgtgc ttgcttcggt cgaatatcat tcacgatagt ccgtttcaga
      121 gcgtccaagt cacctgacag agccgtttgt gcgctcgtag tctgcgactt aaactcttca
      181 agtctagcga cagaatccag cccaatccgc ttcgcttcct gagcgagtaa gctgcttgcg
      241 ccagcgtttc tcaaggcttc ttcagccctg cgcttagctt ctttcaatgg cccgttgtca
      301 aagctattaa agcgctgatt gatagtgtca gacagttctt gcttgacttc ttccgccttg
      361 gccttggcaa gttcgatgcc gtcagaaatt tcctgtctaa gcaatccagc cttatgatca
      421 aagtctaagt cagcattttg aagagccttt tcaagggcaa cttcttgtgc agattctgtt
      481 actccaagaa tggcatccgc tgcgctagat aagccaccag atgttctaga accaccagct
      541 cctgccttat catcgagaat cagagagata tattcttctt ttaaggcatc gaactcataa
      601 gcaatagctt tcttaaatac atcaacatta tgtttccagc tcttgagatt gaccgtatca
      661 cccatgtgaa caacttgccc atcaagttca taagcttcaa tcttgatagc gtcagagatc
      721 ttgtcaatgc cctcgtttga gaacttagcc tgtgcccact tctgcaactc ttcaacggat
      781 tttgcgttgt tgttctcata ctctttttcg tttatgtaag gataagaatt aataagagga
      841 ctatcaacag tcactctgat agttgtttcc ttttcagcac cttcaggttt aaaagtcgac
      901 tttgcgtgaa ttcttgtgac aacattctga ctgttttttg tgcgttggta gttcttcaga
      961 tttttgtgcg ttgtaataac aacaccacga ttctccccac gactcttctt tacagtcatc
     1021 gcaaagttat cacgcaccag ctcgccttcc cacgttccga cgatactatg cttgccgtcc
     1081 agcaatacag agtacagggt ttctgtctca gtcgtgttga atgtcctacg gtcctggata
     1141 tcactgttga atgagaagtc tccaagagct gttttggtgt tttgtaccat gcgagaaaga
     1201 gccatgccac aactctgact agtcacactc atttgtgtga tagatcgttg catcacatca
     1261 tctgaaatgt gataggctgt gatttccaga tggtcattgt gctcaacagg tttcttaatg
     1321 cgaaatagct gcgcaccaag aacaggagtc ggcgctttta tcaacatatc ttcttggatg
     1381 agctgataaa taccagagtc ggaaatggga tatttcacag ttaaggtgaa atcaccattc
     1441 atgatctctt taacaatcgc cgaagtcgct tcatgaagtg gctccccgtt ccatcgaacg
     1501 gttcttacat ctttattaag tagataaagc aattatgccc acccccaaac cgtttcgatt
     1561 tcaatcgatt gaatacctgg gcctaaaaca accccaatat tcttaacttt cgctggatca
     1621 actgtgataa aatcccctga ccatttcact ggcttccctg ttgtcgtttt aaagctagga
     1681 ttgtcaggat tattaaccat cacaagcgat tctgagagtt tttcaagcct aatgacctga
     1741 ccagcgattg taaatgaagt ctcaacaacg ctctgaccaa cgattgtgat tttaggaaaa
     1801 gcaagagcag aaccttgcac attcagagtc ccgcttcttg tcaatctctg agtatcagtt
     1861 gctttgaagt attttgtagg atggcaagtg aaggtcgctt tggtcatgta aagaccaggt
     1921 tgtacttctt caaggtcagt cacattgacc ttatagcacc agagacgagt tgttttgact
     1981 cgctcgctct ctaaccagaa cttctcacgg ataaacagac tcataaattg gttcatctgc
     2041 tcctcagtcg gtttgactaa gtaaatcgta taagatttct tgaccagttc cctgtgcttg
     2101 ttcgtctgaa caattgcccc actgatacca ccatgctcca aaagagctgt cttgctctct
     2161 cccagagcaa ttgaaggaga atcatggaca atgactttaa acggaaaaga cgatgttctc
     2221 acaccgtcaa tcacaagctc attatgcttt atcatgtaac ccctgctctc aattgtgtct
     2281 tacgttgcaa ctcgtcagca atacgctgag ccacctcatc agcaatacga ctgatgtcgg
     2341 cttcttctct gacagtgttg ccagtaatgg taatgttaat ggttggtgaa gttccaccca
     2401 tagtttgagc gatacctcga ccgatggctc caagcgtttt gtcgttaagt ggcaatactg
     2461 cttcattccc agcttcacca ccaaccatta tgttattacc attcattcca aagatagttg
     2521 gtttcgtcat gataccgcct ttggcatacc attcgattcc aatacttgga acaccttgac
     2581 tcaaccaatc taatggattg gccgaaccac tcacgtaaaa gtgaggtagt gggatatgtg
     2641 gccagctgat gttgaagtta aacaatcctt tgatggcttc aatagctgaa gaaacagcat
     2701 cccttgcacc attgatagca cttgaaatgg tactcttgat accttcccaa acacttgaga
     2761 cagtactaga tatagcattt aacacatttg agacagtgtc cttgatgctg ttccagatat
     2821 ttgatacagt tcctgaaatg ccgttgagaa tatttgaaat ataactctgg atagctgaga
     2881 aaatagtctg aacaatgctt tggatagctt gccatacagt agagaatacc cccttaatag
     2941 tttcccaagc gcctgaccaa tcaccattga tgatctgcat aactgcttga atgataccaa
     3001 ggacaacgtt tattgcagtc tcaacaacag tcttaatgat ttcccaagct gttgtaatga
     3061 caagttggat attatcccaa ccagcttgaa tgagtggacc taaaatttcc agaattgtgc
     3121 ttataaccgt atagatagca ttccagacag tctcagcact tgctcgaata agttcctggt
     3181 tctccgtcca ccaagcaaca accgttccaa agatactcat gacaaaatta gaaatctctg
     3241 atacgactgc attgataact tcaagaatcg cattccaaac ggtcgtgacc gcatctcgaa
     3301 aaccttcgtt agtttcccag agatatttta caataacaat aattgcagca actgcagcag
     3361 caattgcaat agctgttcca ataattggca atgcggcaat tatcatttct ccaatagata
     3421 tttttaaaaa ctcagcaagg gcttgcaacg ataagaatat gggggctatg acccctacag
     3481 cagtcacaac tgttcctaaa ataacaacaa aatcttttac tggagcaggt aaggaactga
     3541 acagctcagc tacacctttc acaatcgttg ccaaggtttg aaaaacagga atcatcattt
     3601 ctagaagagg ttgaccaata gcagataatg cattggtccc agcttgtttc agattcccca
     3661 tcacgttttc caggccgtct gattctcttg cagcctgtcc aagagctcct gagagtttat
     3721 ttccgtcttc gaccatctga agcaaggtca attgcttctg cgcttcgctc aagtccttga
     3781 atgactttcc gtacaattta tttgcagcgg cattcctggt cgtttctgtt gcagaaatgc
     3841 caagagccgc atcgttagca aagtttccct tcaaaaaaga ttgtaagctc tctgtcacgc
     3901 tctcaataga tttgtcatag aaggctgcac cgtctgctgc tgccctagtt gcacgagaag
     3961 taagatccaa agcttctgct gtatccaatc ctgaagtttt tgcaaatgaa gccatctgag
     4021 tgaatgatcc ttgcaatcgc tctgggacaa tatccatttc ctgaccaata gcattcaacg
     4081 cttctcttgc ttgggtttcc atatctccga aaacggtagt aaattgagca ttactagctt
     4141 gcatttgagc agctgcttct aacgcttctt ttcctacttc cacaagcttt tctgaaatag
     4201 cactcaactt ctcactaaac tgttgaagta gttctgctct taaatttctt gagatttcac
     4261 ttaaactttc ttgagtgcta tcagcagcag actttgttcc tttcatctca tcattgagat
     4321 gattgaatgc tgtcttagcc tgatttagct ctgcttccat cctgttggct tgtgtggagt
     4381 tctcaccaaa ttctttttta gtgatttcca attgctgttc tagatttgaa atctgtttac
     4441 ttacaatctc agactgagca ccaatctttt tctgagcaag agcatttctc tcggcttcac
     4501 tagcatttga acccaaagcg ctttcttgca gtttgaatga acttgtcacc ttactcatct
     4561 ctgaagcaag ttgactctgc tcattttgca actcttttaa ttggttcttg ttgttctgag
     4621 tagcactccc attctcatta agcgcctgat tcacatttgc aagcttaccc tcatatcctt
     4681 ttaggacgtt ttgagtaact tctacttcac gttggaaagc acggtactgg tcagcaccga
     4741 tatcgccatt tttgaattgc tgttccacct gagactgagc ttgtctcaag gtttctagtt
     4801 tctctttggt cgtcgaaact tgcttttgca agacctcttg cttctgagtc aggagcgtta
     4861 cgttccctgt atcaaacttc aatgctttgt caatctgtct caactcctga cttgcatcag
     4921 tagcagcctt attgacattt ttcagcgcct tctgtaaggg ttgcgtgtcg ccatcgattt
     4981 caattttaat acctttgata tttcctgcca tatttcctcc tttctcaaaa aaatagaaaa
     5041 gcgctgagag aacttctacc actgataatg cagccagacc aaggaacttg gtctcagaat
     5101 cgctctctca gcactcattt ttctttaaaa accgtcaaaa tcagcttgcg tggctttccg
     5161 ttcgccaccc ttatcctcgc tccgtagatt cacataatcc gtctgataat ccagagccat
     5221 tccgattgaa atgtgcttta gatcatcaat agaaagacca gtttctttac agcaagataa
     5281 gtaggtttct actgtgaaga tttcttcgct agctgattct gattcatctg gtgctttttt
     5341 gtcgtcatgc tcgcattcag catttccatc aatacaggcc caacttcctg aatcggaaag
     5401 acttccattt ccatgaagaa ttgttcataa ggcttgatgt gaggattttc agatttagca
     5461 aaggtccaaa aaagacggtt gaaaaaggtc atgtcaaaat ctgacaacat cgaaatatca
     5521 atattagtcg ctgtcaattc cttgtcggtt tccaacttgt tcaattcatt catgaatgat
     5581 tgatttttca acatcgagaa caaatcttga aaataatctt ttccaaattg ttgcttgtaa
     5641 gcaataggag tatagccgtt ggtccccaac tcatactcct gatcaccaac caaaacgatt
     5701 ttacgcatag atcttctcct taagctgcaa ctgcagtagg ttcatacact ttcttgaacc
     5761 agttgtcata cgcatccttg tcatcagctg atgtgataga acgcttgaca actgtatcca
     5821 atggacgcgg gctagctttg aaactaagtt cacgttcgtt ggttgatgtc ccgttcttag
     5881 tttttgagcc aagagatggg cgactggcaa aacagtagta catcacatag cgagtcttgt
     5941 ttttgtcgcc ttcaaactgg aacatcattg cgaactctgt caagctcgca tctgcttttt
     6001 cagtcataac accagtttga gggtccttga tttcaccaag aatttttgtc gcaaattcat
     6061 caatgatgtg tgggatttta agtttaccct catatccttc gtttgaattc atgaaatggt
     6121 aatccttgtt gtctgctttg atagtggttg tttccccttt agtatcaagt gtcagatcta
     6181 ttgctccagg aaaacgaaaa acatcgccgt aagtgataac tccatctgct gcaagtgtct
     6241 tgataggtgc gatatgtaca ttttctaggc caaaggttac tttattttct tgagtcatgt
     6301 cattcctcct tagtatagat agactgtgta agacttgaca tagagtcttt cagtctcgat
     6361 aaatgtttct tcttgaactt caaaaaagag cttgtggttt gcccacagct cttccagacg
     6421 ttcttccaaa tcttcatcct tactctcaaa agccagctca actgtcacgc tcttaatctg
     6481 atgattaacc gtgttgtcag ctgcattgat ggctggaatt gattcataat agaccagata
     6541 aggtaggtta ggagcgtttc caattttaaa cgctcgataa gtgacaggca agtttgcctg
     6601 ttccaaaata gcagcaaagt ctgatagctt catttcccaa tctccttgat tcgcttctca
     6661 aagttttctt taactttctc ctcaacaggt ttaatatgtg gaaatgcccg actacgaccg
     6721 ccatttctca aaacatgccc attttctagt aagtgagtta aacgataggt tggagctgcg
     6781 ttgtagatga cgtatgaccc cttagcattt ttcttgaagc gccaatttct agcatacttt
     6841 ccatgacgtt ttggactagt cgcttttaat tccgtaacgg cttcgtttac aacgtcctca
     6901 gcaatcaggt caatcttatc ttctacctca gcagagtact ctgccattgc ctttgcaatt
     6961 tcattcgcta aatcacttgt taagctcatt tcaacacctc tgacaaagtc aactctaaaa
     7021 tttcagaatc aataggatag gttttcaaga tatgatattg cttgccttcg aacttcgcaa
     7081 gctcttgatt ctcatactca aaatttcgaa tctcaacgac caagctcggt ttaagcccta
     7141 cctgattcgc ctgataaaat tcagagcgag tgaccctctt tttgcgacat aggagagtaa
     7201 cttcaacatc ttcagagatt ggttgtagta gtttatcctt acctgtgact tccttagaga
     7261 tcagtgtgat ttcatgattc cacattcttg acctctttct ttgatgctat ctgtaaatta
     7321 tgcagtcgcc actgaaggtg acgtggcata tccaccccac cctcatagcg aaaggctgca
     7381 aaatcaacta caaacatttg gtcttctgag ctgtctaagt caagtagaac tcctaagtta
     7441 tcttctagtt ctgttttgac agcttcgatg attttctcca agggcttatc gcgtagtttt
     7501 gttgctatac ccaattttag cttaagcaat tctaataatt gaacattgtc cataactact
     7561 cctcgtctcc ctcttcaggt ttcccatcta ctacctttgt ttcttccttt tctacttctg
     7621 tagttaatgt gtcatctttc gacatatcct tatcttctac attcatcaaa aagatagatc
     7681 ctgcactatt tgccccattt agcagagtct ccgcaaactc tttatcaagt ttgtgcttaa
     7741 ctctagggta attatcccca accttatagc aatgtttctt tgggtctcgt aagtccttaa
     7801 aaggacggat taccttatat accatttact acctccttac aaaacagcat ctgtataggt
     7861 cacataaaat cctgcatctc cgtctacttt tactacatca aaacgattag cggttgccaa
     7921 atactgaccg tagattttgt catcttgcca tttgacagta gtctgcgcac ggtcaaacaa
     7981 tgtcgcaaat tctccaacgt caccgatgaa ggctttcaaa tctcccttgc tttctccaat
     8041 gatatcatct ggataaacaa caataacacg accagcaaac ttgtagccag ttggagatgt
     8101 gatatctgtt tggagcatgt agcgaccgtc cttgtccttg attttatcaa gagcagcgaa
     8161 catagattga gtacatacga tagttgcatt gtagtatgtt ttcaattcca cgttgagaat
     8221 atctttcaag ccgtccaaac cagctgcgct tttagatgta gctgtcttga gaactttagc
     8281 gatctcttta ttcttagtga tacgttcttg gttcttagtt tgtttagcaa ccaatcccat
     8341 gacatcgtag tcagcgtcat caataaattc ttgagatact ggcaaatgac cacgacgtgt
     8401 tttgatttca tagttcacct ttgtaaaggt tggtttagcc aattcagggt tttcttccaa
     8461 ctcttcaaca gtgttcattg cttgatcagt caattttaca actgaccatt taccgcttgc
     8521 gttcttgaca ttaacgatgt tgaccaatga agtcagatct gtcttgtctt gtttcgcttc
     8581 tttaggcgtc atcaattcaa caggaatgat tgcttcccct tcggcagatt tgaaaccatc
     8641 agcacgcgct tcttttgttc gtaagtaatg attaaatgct tcacgttgtc ccaatgtctt
     8701 tcctcctcgt tcttccattt tccccggagt tggtgcttta cgattttgct ctttgatttg
     8761 tttatctaac tcatcgatct cgttttcaag ctgtactttt tcagcttctt tttcttcaat
     8821 ttccctttga agaccgtcta cagtcttttc aactgctaaa acttcttcat catttttagc
     8881 acgatccaac ttttctaact caacaaccga acgtttgttc aattcttcga tagtttcttc
     8941 caactcaact accttagttg cttttgctcg catacgagca ccaaagatta atgccttatt
     9001 catagcttaa atttctcctt aatttctttc ttgcgcttgt ctagcgcttc acgattagca
     9061 cgcttctgac tttcgaagtc tttttgtcgt gcagcaattt ccgtttgtgg ataggctggg
     9121 aaagtacatg gactcacttc aaagatttct agctctaaga cagtgtccaa atacgaacca
     9181 tcttcacgtt cttccgtttc gatttttacc gggataaagc caaagctaca ccctataaca
     9241 tctccacgct tgacacgggc ataggcccca acagcttgag gatcatcctt gttaatgatg
     9301 atatctccaa aaagaccaac atcatcaaca cccagtgtca gagttccgtt acctgttcga
     9361 ccgagaacaa ggctatcatc atggttaaat aaagctctga tatcagcgtc tgtgacagct
     9421 ttctcaacac cagcacgctt gattacttca tgataaccac gccacaactc cgtctcttca
     9481 tcaaatttta taaaatagcc actcaacacc aagtctccag attcttcttc tctcgtttga
     9541 aattgagtgg cacgatagct atttcgcttt tgcattctct tcctcacctc ccttcagttt
     9601 attttgatct cctagtttct cctgagggag aaagttttca agaacaatca actcttccat
     9661 ctcaggatca ggagccatcc ctagccaatc cctccactca ttacgacgca ttgcagcact
     9721 gtttgccatt tgttgagcaa cagcagacaa ctccgtaatg ttgtaagaga agagtgaacg
     9781 aggatttagc ttgaagtaac gattgctaga caaaagtaag tccctggtta gtgtttgagt
     9841 aatagtggta gcaatactca tgacagtcgt atttacaaag ttgttatact ctgtcttgtt
     9901 gaactctcct acacccaaaa taaaagcagg tactcctaat agacctgcaa ctgttctttt
     9961 atccaattcc acagactcgt ttaaagcgat gtccgttaga ctaagcggtt ttacctgctg
    10021 aatgtccagc aatgcctctg gaacaatcca tggagcgcca accctgctag tactcaaata
    10081 cttctcagcg atacgctcac gcccttgctc cgaatctagt tcagcactag acgagtctac
    10141 tttaacgata agactaggaa tattcttccc gttcataaag ctttttttag tcttggtagc
    10201 catgttcaaa ctttgaacca catctgtcaa cgtcacccta aaaccagtac caatgtatag
    10261 aatatctgga tctggattga tgacgaagtg gactacttca tcaggggaat attcttcacc
    10321 cctaaatgag attacatagg aatccttatc tgtttggaat gaaacctctc tcatcggaaa
    10381 tggtcttaga ttagaaatat aatccgtaac aggttcatat tccacatgta ggacagagtt
    10441 cccatcgcca tataaaagca aatcgcgcac aatcttgaaa atccatgact tccttgtcat
    10501 gtgtttacac ggattgatat caatctttct agcaagccca tcacggattc tgatatcacc
    10561 tttatctgta ttctccatca agtggatggt catattagag accaaatcag caatcttatt
    10621 aaccgctgtc accacatctg gatttctggc caaaggtaca tacgagtcca tcaggtttga
    10681 caaccctaaa tctgaatgac tcagcatgtt gattgactta cttggcttgt ttcgtttcca
    10741 aaacttttca aaaataccca tgtttcctca cctcctttct aacgaaaagt attttgaaaa
    10801 agtgaatcaa agtgtttatt tcttacgata ttctgactga catcaattac ctgtttttcc
    10861 cattttactg tttcagccat caaatttttc atatgtcttt gacgaatagt tactacttcc
    10921 ccgtttaaaa ttactttaac tcgccctttg ttgatcagca agttaatttc atgttctgat
    10981 aatactattt cattcataat tcacctaatc aaagaatctc atcacatcac cacccttgcc
    11041 aagattagca agagcctgta tacaagcaaa gacgctggca tcaaacaagt caatccttgc
    11101 agtcccaccg tctccatcta atttctcata ttgcacagca tcgtccacct tttcaattgc
    11161 tctaacattg ctcacacagt attcgtaagc atcagaatga agatagtaaa attctttatt
    11221 cttaacttta aactcaatcc gtctgaatcc ctctgatttc agataaaaaa gctgaggttg
    11281 gtcaatcatc ttgaaccgag cttgtttcat cttcgtcaag aactcacggc caaacttcct
    11341 atccattccg acagcagcaa tcttgaaccc tttctccctc atcttgatga accatttgac
    11401 gatatcatca tagaggacgg tcggagtgtt gctcatcgtc aaccaaccgt cagactgcca
    11461 cccaaagagt ggaatcccgt catcattagc ttttttctga gcattaatcc gagggaagaa
    11521 agcatgtgtg atacagatat caacgtcttt ctcaccgtca tgatagacac catagagagc
    11581 agcagctgtt aagtcgtgca atcttgacaa gtccgcacca ccataccaac gaataggcaa
    11641 acgtgccagc tcttctaaac tccaatcgta gcaactatcc gacgcaataa actcatcagg
    11701 attaaaataa gcgttcatag agttagtgaa gatattcaag gtcttattga aaaactcatt
    11761 tcttgtctga ggatcattca tagcctgctc tgcttcttcc ttgagagcct tgagcgaaac
    11821 cgtgacaccc catgacggat tagccatctt gaggatattc tcgtccagat agttcaccac
    11881 gtccccatca gcagattgat tagccttaca gatgaagatg aaaaatgaat catcagtgac
    11941 taattgcttg agcacctttt gacagtattt cagacggtta gcaaggaacc cggtaggaat
    12001 atccccagct gtagagataa caaaaagcat actgttacgg tatgctgaca ttgttttttt
    12061 cataagacca tgtttcttac tgttcctcat tgtgtgagct tcgtccaaga tgataacatt
    12121 tccgttcaaa gagtccagac ggctctcatc attcgctaaa gcctggataa agaaagaacc
    12181 ttcatcacca aaattggcgg tgatagagtg ttcttggtta ttatccttga tacgaatgtt
    12241 cttatcgttc cagcgttcaa cgttgaatct taaaaaacca aaagcttcca tcgcttgctt
    12301 gactgagtta gcaacgatat agcatttgga accgctatct gtatctaata tctgataagc
    12361 aagtgcgatt gcagcagtaa acgaggtctt cccattcttc cgagcaagca tgatgagcgc
    12421 ttctttgaac ctgcgctcat tcgtaccctt gtagtaaaaa ccaaacagat taactacaac
    12481 gaaatgttgc cacggttgca aaagcaatgg cttgttacgg atagataccg caaacatatc
    12541 atcgccctgc tgatgaacta tcacgttctc gataaagtga ataacaaaat ccaccatatc
    12601 ctcatccatc tcaaaggttg gattttctaa atcacggaaa aaacgttcag cagcaaggat
    12661 gttttcttcg caatgttctt ctcggtgagt taagacgtgt tgagcgtatt ttttagcttt
    12721 atcaagatta cccattgcca gtcactcgct tcttcttgat ttcgttcttg aacttcagta
    12781 cctcagtaag aactgactca ccctcttgtt ctactacctc accgagcgac ttaggattca
    12841 tcatcaactg attagagtag ctgagtatat ctttcctcaa aatttccatc gctgtcaaga
    12901 ttggaacttt gcgctcattt tcagcaccag ccttattgac gtaggtgtct gttactggat
    12961 aacccatatc aacataatct tgagcaagtt tctgatactg gtataacata cctgcaaaaa
    13021 tgtcaatgat catttcgaac tctttacgat aagttcccaa gtctttcatc tgcttgacca
    13081 cttttgactt aatcgacttt gctgtaattg gtttagccaa aaactacctc ctttcgtcaa
    13141 aatcgcttag tttttacccc ctttttgttt gaaggccccc gacttggaaa aagttccctt
    13201 caccggtacc ctactggcca aaatgatttt tcaaaaagag gggggattaa aaattttcat
    13261 ttttcatttt tgaaaaaatt taaaaattct ttttttcttt ttttctgcca atacaatcct
    13321 tggttgatta ctctatcgtt cactctatca tgaaacgtat tatgtttctt attcgtcaat
    13381 ggcaagcaat tccattcaac aaattcaagc tcaggatatt cagacacagg aaagatatga
    13441 tgtaccattt ctgcttgaac agaaattccg taacgcaaac tttcttggca aagataatca
    13501 tgcttacgca ttatcctatc acggaacttc tcccacttct tagatttcaa ggatggtctg
    13561 ataggtttgt tatacatctc aaacctcctt tctcaatgct aaaagggaca ggcctttgac
    13621 ctatcccctc ctcatacaag aaatctatgc taccataata aacctttttt tgtgagactt
    13681 caagatgtct tttgtctcaa tcttatttac aactcacctt tcagtatagc gtactgttct
    13741 aagataattc ttctacgtcg atagattgta gctttgctca tgaatttctg ttctgctatt
    13801 tcttcccatc tcagttgagg atatctccag cgcagattaa agatttcctt atcttcatca
    13861 actagattga tcaaaagttt gttaataata gctttgaacc cttcgagaaa ttttaaggtt
    13921 ggatcatctg cgattctgac tgcgatagtt tcggtaggtt tgcttattcc tacgctggga
    13981 ccactctgag catctgggtt tcgagtttct aattctagtc ttctcaaatc tattgtacgt
    14041 tgaatgtttt gaaatttgaa aagttctctg tctaatgttt tgaggtcttc gtcgctcaat
    14101 tttttcaatt tccacctcca agtttttcaa aaatgtaaac aagttatcaa aattgtcgag
    14161 atcggatatc tattcctact tgtcttcgtt ccataaaatc taactgtttg ttcagtaata
    14221 ccaaacatag tattttaaaa tatctcactc gttgacctcc aaaagctccg gattttcgta
    14281 gacattgccg atgatttcct catattccgt ccacgcatat ccatctccaa atccttttag
    14341 gtatacagca ggcattccac ctatgtatgt gccaccgtat tctttttcta aatatacttc
    14401 atggagacat cctcttgtac atttaacgat gtcaccgatg aatacctcct tgccgttctt
    14461 gtctctgagt cctgttgatt gtcctaatgt tgctggattt acaggacacc aagaacctat
    14521 agtaatgtat tgttcattgg cttctaccac ttcgttgata ataaatgctc ttcctctatc
    14581 ttcaattaaa tgtccgtatt gccattctcc tttgcttttt tcgtcaatgg ataaccctct
    14641 aaattttggt atcatgctaa cactccttaa ataaacaaac tagctaacca tatcaaaaat
    14701 gcacatgtaa tgatttttga aatactgctc tttaccgcat acgaataatc ctcttcagat
    14761 tcttttttgc tagatagcac aggccagatg aaagatagta gtgcatccat ccctaatgct
    14821 tgccagactg taattttacc aactggaaca attgttgtga taatctcatt ccatccatac
    14881 tgaactacaa acggcgatac aacgattaca aataccgccc caataatgat tcctagtctt
    14941 ttcattttat aaatcctcct ctttgacgaa agcaccatca atccaacgac cctaccgttt
    15001 tctaaatcac ggtcaataaa ccattgtttg actttctcta ttgtgttcat gataactcct
    15061 ttgctattgc tgctatgaca ttaactgtca cgctatttcc tgcttgttta tataattgac
    15121 tgttagagtt gacctcctgc gccttatcaa aagcccaatc aggaaaacct tgtaacctcc
    15181 agcattctct aggtgttagc tttctaatcc taaatccatc tgataaatga ttgttttcgt
    15241 gatagctatt gctagttaaa gtaggagcga tgttatgttc tccgccttta ttataaccat
    15301 gcccacgttg aataatttta ggctcaagtc ctccaccttg ataggctctg atagttggtg
    15361 cgatgccgtc tgtttcgtaa accaccccac attggttaaa attgggttgc aataccccaa
    15421 attgttttat agtattgctt tttatagcta tcttttgccc ctctcccttg tttgttgtta
    15481 gcgtgggagc tagaccatca gcttgataga cttccccatt cattccgttg ccagatgggt
    15541 ttatattccc aattttcatg actgattggt tactagttga ctgatttttt ccgctgagag
    15601 gaaaaattct tctggtatgt tctcttctaa gatgtccgat aatgaacact cgttcccgat
    15661 tttgggggac tccgaaattc ttgctgttaa gcacttgcca ttccacgttg taccccagtt
    15721 catccaaggt tgagataatg gtctcgaatg taactccatt ttcgtgattg agcaatcctt
    15781 taacattctc aaggaataga tatttaggtc tgagaataga tgcgaaccta gcaatttcaa
    15841 agaacaaagt tcctcgtgta tcttcaaaac ctcgtctgtt tcctgcaatt gagaaagctt
    15901 ggcacggaaa tcctccacag ataatgtcca cacttccgat tcctcgaata gattcatctg
    15961 atactgctgt gatgtcatgt aattctattt ctcctttcgt attgtgtatc gctttatagc
    16021 tttctctagc aaacttatca atttcacaaa agccaataca tttatggccg gcagactcca
    16081 ttcctaaacg aaaaccgcca attcctgcga ataaatccaa gaatttcaca acatcacctc
    16141 atccccaact ttcactttct catacacgtt cttcaaaata actatgaaat ttacttaaat
    16201 tgacaatagc gacctcttca acagaatgct ttccgatgtc aaagtctgga tcattcttcc
    16261 caaattcttt ttctatagct ttttcagcca gaaaaggcaa atcgaatata cttgccccat
    16321 ttcttaaagc gagcgcttga ccgtatttgt tcactattcg ataccctata tcaaacggtc
    16381 tgatttccct tgggactttt atgcatttac tttgattctt cattccttct tcaagtgttt
    16441 gtgtcatcac tccacctcct caatctcaaa cccttcacaa tcaaacaccc agccgatttt
    16501 tgcattttct agttctttac gggtgtggga atatatagca tcgtctaaac taaagctttt
    16561 tgtaaagaag tacctttcca aaagttctcc ataaaccaac atattttctt taatattccc
    16621 tttaatcttt accagatacc gcttctcttt ttcgacctca tagccgtcaa gccatgcgcg
    16681 agcgaagagg tcttggttgc tcgccttttt aatccataat ataagatcga aactttggtt
    16741 gttttctttc ataaagtttg gagtcatagc agtatataga ctagttgtta aatgttcttt
    16801 acaaacctca atccaatccg ccacaaactg cggaattttg actttttggg gttcgtctag
    16861 ttgtttcaac tctttcaaaa aaagttcaac cattgaagta taaggtacag gttcataaaa
    16921 agggctgtgt tcattccaca attcttcata cttcttaatc aatgccttaa tattcatctt
    16981 agtttcctcc ataaatcaaa taaactgcaa taactacctg agccatgctt ggcgaatagc
    17041 caacccaatc atcaaactcc ttagattttg gcaaccaacc cttagtagct cccaaatcat
    17101 agtctgtagg cttttcatca gcgaagatac attccatcgc tcccataaac gtcataccat
    17161 cttctgccat ttcccaaaaa tagtccgccc ggtctttcac cgcttgtggt aaatcttgct
    17221 tgggaggttg cggcttcccg tcttctaccg tccagttgta tacttcatta acttttcgct
    17281 ttaactcttc catcatcttc caactcctcc actttccgtc tcaattcttt attctttttc
    17341 ctcaacaaat cgcgctctag cgctctaata cgtctcttgc gtgcatcgca cggtttcgaa
    17401 tactcgatta tcttctcttc gttttgatcg atcgtgcgtt tcagtccgtc aatctcagcc
    17461 tgtttatcgt acttcatctt ctaaaaacct ttcaatagct tctctgtagg agacttccac
    17521 cagaccgtct aagtcgttca gggcttcaat atagtctgga cgaccttgcc cgtactgctc
    17581 tttcaaaaat tcaacaaaga gatgaatttc ctgataggtt actccaacca tgtttcttac
    17641 ctcccactaa atcagaaatt cccatacatt tgtgaaaatt ctcaaatgct aaatccatta
    17701 aaatattttt agccattatt tccctcctga aaaagtcgct aaatagtaac aatccttaga
    17761 accgtagtca aatcgtgtcg tccgctgacc aatgtgcttc tgaaatcttg gatgagtgat
    17821 agccgagaaa gcccactgat gatcttccat ctgctcaatg agatcatcga cattgtcaaa
    17881 cctcccaagg taaaacttgc agtgcccgtt atagacgaag taaagctcta acatcaatac
    17941 ctcctaaaat aacttcaact gcttctcata agcatcaagt ctctgttgag cgaggttgaa
    18001 aatactttta tcaatctcgc atccaacata gtcaaatcct agctctccgc aagcaatcaa
    18061 gctacttgct gaaccgacgt gagtatcaag tattttatcg ccttcttttg cgtaagtttg
    18121 caataaccaa aggtaaaggt tgactggttt ttgtgtcgga tggattctaa cctcattcaa
    18181 tgccttattt ccttgttgta tatgaccttc agagattgat ttaccttgca tcataccatt
    18241 ccacatgtaa cgaaatagcc tagtactatc atgataactg cagtacgcta tctcacaatc
    18301 tgaaaaactt gattgaccat taactttgtc ccacacaata cggccagaac caaaagaata
    18361 gtcgaagtag ttcacacccc aaataatttg atttttagat accctgaaaa gttcatcaaa
    18421 ataatctctg tttggaattt tccactctga gatttcgcca tagagtctgc ttacacctat
    18481 tggactgatt ttccgtccgt aaaatttcct tttttctgga ccagaaaaat atggtggatc
    18541 aacaatagct aggtcaaagt aattatcagg atattgtttc atgatgtcca tgcagtcacc
    18601 atttaagaat ttcatcatca ctccacctca acagggtaaa agttcccaaa ggaacccctc
    18661 aaagcctttc caacctgtac ggcagccgcc cgagaaataa accgcatggc tttcttctcc
    18721 tcggaacacg aaatatccaa gccagtcaca ctgataattg cagaccttag aaacggctta
    18781 tcctctcttg tcccatgctt taaaataaac atcagccacc cctattctaa aaatattgct
    18841 ttcgcttgtt tgtcaaatca ttgaaaacca tcaaatggtc tttatctaca cccttcatta
    18901 gtctggacat gaaaggtctg ccatatcttt tttgaatatc ggcagaaatc aaatttgtgg
    18961 taatgattgt gtttgaacgc ttattcaaga tattgtagag aatagtaaat gaccattcgc
    19021 tatctttttc cattcctaaa tcatccaaaa ccaagaactt agcactagca attttattga
    19081 ccagaaactc ttcctgacta aaatcagctt taatcttcat cagcaagtca gttacgttaa
    19141 taaaaatagc aatctctttc gtgtactcag atagagcctt aaccatagca aaagccaaat
    19201 ggctcttacc tgttccagct tctccttgta acacgatgtt gttcctagca ccctcagacc
    19261 actcacgaca aatcctcttt gcaaaagcta gcttttccgc ttctttttcg gttggtgttt
    19321 caaaattgtc caaagtagca tttttcaaaa cttcatcata aagagagaac ttttcaagat
    19381 agtatttcct ctctcgctca ttctcagcat tagccagttc attcactctt tcctgatttt
    19441 cttcatgaat ccgctcagat tcacacatgc gacatacaac actctcggtc ctcaatatct
    19501 ttatcaaagg gatgttatgc ttttcgcaga actcttcttg ttgttctgta ttcctacgat
    19561 aagataaggc gatttcctca aacacattgt ctaccatact agccgacctc cgcattcatg
    19621 ccagctagcc atttcagaca agcaggcaac cacttgatga attggttggt ctgctaaaag
    19681 agttttcttc tcgtagctta acggataata gtcaatctcg aattgttcaa ttagttctag
    19741 tacccccatt cgtccttggc ctcctgttct tctttcttat ccttgttctt cttttccgat
    19801 tgacgaacct gttcaacagt ggtaacattg ttcatctgcc aatttcttaa aatcccacca
    19861 atatatttga tgttcggctt tcctgagtta atagcagtct tcaatgcttc tttcaccaaa
    19921 tccacatcat tctcatttag tagatggttg atttcttcaa tttcaaatcc agataagagt
    19981 ctacgaaact cagattgaaa aagttctaag atattttctt cactaccact actagtagta
    20041 gttattcttt tcttattctt atctttatct aatctattct tattcttatc tttatcttct
    20101 tctagtgcgt taccgtccgt tactgtaacg ttacctgtaa cgttaccaag agcaagattt
    20161 ttttgttttt tacggtattt ggctacacgg ttacgtgtct gttccttgat tttctccatt
    20221 ccgtcaacgt tttgatgttt ttcccaattt ggcaagctaa taataccatc gataatctca
    20281 atcatcccaa actgttcaaa aactccaata gccattctta ctgtattcaa tggtctacga
    20341 aaaatagtag ctaacatttc atctgtatag tgaaccttat cagtcatcat caacaaacca
    20401 ttactgttat gttttccagc aagtgtcaaa atcttgaacc atatcactaa gatggcatca
    20461 ggatcaggca aggcatcaat caggcaaatc ttttcatcgt caaaaatatc ggttgtgatt
    20521 tttatccact taatttcaga cataccgagc accccacttc ctgcgattgg cacgatactt
    20581 catacgcata tcctcataga tgtgcctgcc ttccagctcc attttttcaa tctttagcag
    20641 cttattttta agggtcacat aacgatagtc ctttgctagt ttttcatagt cggttaggta
    20701 ttctttgact agtaatagat ttttataatc gttttcccat atcgtaataa aatgtcttga
    20761 agttgattcc cttccttcca gttctttaac aatcataatc aggttatcca gcgattcaat
    20821 caattcttcc atttcctgcc ctcctcatta cagaagtctg attgcagact gtttaggttc
    20881 tggcaaagct aatggctcag gacgcaatcc ttgaggcggt tcgttgtcgt aggtaaagcc
    20941 cttgaacgga cgacgaatat tcttgcggat ttcttgacgt tcagcttctc taccacgttc
    21001 gtaagcatgg ttatagcctt gaataatcat agacgcaaat tcttgctctt ctcgtctttc
    21061 ttcttccttg cgttgttcct gcaatttgat atgacggcaa gcccctgcaa atccaagcag
    21121 taaggctcca acccccatca actggtctaa aatcggtggt tcaaacattt ttatctcctt
    21181 atcctctttt tgtgctataa tatagtcaaa taattttgct aagaccttgt ccagaagcct
    21241 tttagtaaag ttattatatt tgattagaga gccattcttt gatggctctt tttgaccatt
    21301 tcttaccagg taattccttt ggaaatccct ttaagtaacg ataattatct gaaaaggtgg
    21361 catacttaat tcctagaaat tcgcaggtag tgttcacatc catcaactct ggatagtgat
    21421 cactatcttt ttctatttcg actagccttg tgattgtgtc cttgataata gatttaatcc
    21481 attcagacag tgaaagtaga acattgtcca tcttcttccc ctttctagac gtcatcaaat
    21541 gagttcaatt tcatgatttt catcttggta ttagtgcttg gctcccacgt catccagtaa
    21601 gcaagagcgg catccgcatg cttcttgggt agcaagtcat agcgactaat gttgaagtgg
    21661 tctttgaaat caatctcagc ttgtctaaag actgattgag caaaaacctt atccgcataa
    21721 gcaggactgt caataccgcc aaggcaagcc acgacccgag ctttgcgctt cttcagtaac
    21781 gactgagcat agcttggatg aatcggttgc tcactcttta gatagtcgat atcttccagc
    21841 atggtagcct gttgctcacg caatttcttt tgaccagtga atagagcgat aaaggcatcc
    21901 tcgtccaagt cctcgcggat aaatccgccc tgcttgcgaa tagctggcaa gacctctgaa
    21961 gtcacccagc gcttgaattc cttagcttga ggcaacttgc tggataagat gagagagtag
    22021 agaccagatt cgttgatgat caacatatcc tgtgttccgc cactagtagg gatgccctgt
    22081 tttagggcgt cctcttcatc aacgtgaaga gtaatcgcat ttctagcctt gctatatcct
    22141 aggatgtccg ctacatcctt cccgacaaac cacggctcgt catcaattgt caaagtacgg
    22201 acttcttgtc cgtgaaaatt aaaaatttcg ttcatagtat tcctttctaa atttggtata
    22261 atgaaaataa aacgattgga gaaatcttat ggattatcaa attcttattc aacctgcaat
    22321 tagtgtcatt cttgcaataa tctcaggttt atggtcatat attgcatcaa aagctaacaa
    22381 taaagctgag attgaaaaac aggccaaaga acattcacat atcgtagaga aacttgaaaa
    22441 agaatttcat tatcagatag atactctcaa acaacaacac accttggaac ttgaaaaagt
    22501 caaacaagct catgaattac ggttacaaga acttgaaaag gtatctcaga ttgatactga
    22561 aaccgacaag gctatgaaga tgaatgatct tatctacaaa acttttacag gcgaagttga
    22621 tttggacaaa gctttaaaat tagctgataa agctaacaat cacaaacaaa aactaaataa
    22681 aaaatttatt caaaagactt ctaaaaaatc ataaattaaa tttatttcta attctttcaa
    22741 gctcatcatc ttgtattttt ttatactcgt tgatgcgctt ttttctgtct cttttgctag
    22801 cgtagtatgt agcaaaacca atgataagat tgattatgac aacaaagtaa aaccatacta
    22861 gttcattcat ctccaccctc ctactccagc accttactgc cgactaccaa tcgtttaacg
    22921 acaacgtcca tctccttaaa ttcggcattc tctgcacagt agcggacgct ctcactgatg
    22981 atgtgacaaa tagatacccc gtactcgttc gccaactccg tagcaatctc ccaggcatct
    23041 ttgtcaatcc gtgttacttt ttgcgctgcg ttgttcatac tatttcctca tgctctaact
    23101 attttcccaa gggttttcaa tacccaaaat atctacgact ttttctttca cataatcact
    23161 tccttttcca tacttcagca gttctgaaat aactgatgat gttacagata cttgttttgc
    23221 caattcggct tgagtcatat ccagctcaat caaacgagtt ttgattttag ccttgattat
    23281 ctttagttct ttactcatat ttttcctttc taaatttgat ataattaaaa taaaaacgat
    23341 tggaggagaa taatgaaatc ttcatttttt cactatctaa aggaaatcat cttatatggt
    23401 gtcctagttc cgattttgac agttatcgta ggatttcacc tttttactac cttctatcct
    23461 atttctgatt cactgttaac agatttcttg aaagctttta cagaacaaag aatccttgct
    23521 attgtattcg ttcttattct ttgctttata tttttcggtt caatttatta ctgtatttca
    23581 tgtggacgaa atagaaaatt gatacctgat aaagtttata cacaaatcac aacttacatt
    23641 tcatctgtag gtttagggac tgcagtgata gctgtcgcta ccttgactct cacagaatta
    23701 cagttttcaa tccttacagg ttggattgct tttatcgctt tgttgttgcc acttctgaag
    23761 tttaaagtcc aatacagcac ctatcaaagt agcgacaatg tagcacagaa aactaccgaa
    23821 aacaaagcca attaagaaag gctctaccat tctcctctcc ccctttcttt taaaaaatta
    23881 tctaaaaagt tagcgaactt cttgacaact ctagtcaaat gttttaaaat aaaaacatag
    23941 agaaaagacc tactaaaagt aagttttacc tacagtaaac ggacgccaat cagttttgta
    24001 aggctttatt ttttagttgt ctagttcgct aactctttag cttacgaata ttattttaaa
    24061 acatttgact aaataagtca actattttct acaaatattt taaaattatt tttcgtttgc
    24121 ttagaaaggt tgaaaaatta atgtttgtag cattcgataa aataaaagaa ttagctgata
    24181 aacaggggat ttctataaat attttagaag aaaaacttgg ttatggaact aatactttat
    24241 atcgattaaa aagaagtaat cctagttcaa aagttttaaa agaaatagct gattatttta
    24301 atgtaagtgc tgactaccta ctcggacgga cagataatcc tgctatcgct ggaagtgatg
    24361 aatttgctca agtaaatgga caaatcatag acttacgcaa agcagcagcc aacaccatgt
    24421 tatttgacgg aaaaccacta aatgaagatg atatcgactt catcacatcc gttctatccg
    24481 cccacttcaa aagcaaagga gaacgctaat gactatcacg atcaatttca cagaaaaaaa
    24541 ctcctacatc acggactacc taaccaaaca cggtatcgac acaacaacca tggattttga
    24601 cgacttcatg gtactcatgg aagatatcga agacgcacga gcagccgacc aagcctatat
    24661 ggagtattta gccgacccag ctacttatac catggatgag gtcttggatg aactaggact
    24721 atctcgagag gatattgctt aatgtatcgg ctagatattg ataaaaaagc tctcaagcaa
    24781 cttaaaaaac tagatacccc aaccagaaaa caaatcctat cctggcttgc taaaaacatt
    24841 gaaaacacga ccaatccacg acaacacgga aaagcactaa aagccaacct tgcaggttac
    24901 tggcgataca gagtagagaa ttaccgcatc atctgtgata tccaagacga taaactagtc
    24961 gtcctagccg tggaaatcgc ccaccgcaga gatgtttata aataacgaag gagaactatg
    25021 acactcgcta aactctgcga agaatatcaa gtagagcttt gtctcttcga cggttcaaac
    25081 tggcacagta gcggtttcta caatccagac acaaacgtac tcgctattga ccacaactta
    25141 gctcctgaac aacaaatcca agtcgcccta cacgaactcg gacacaaaga ccatacacgc
    25201 tcagagtacc aaaacgctcg cctacgctgt gaaaacgaag ctgataggaa tatgatccat
    25261 catctcgtga aagacgcact agaaagctta gacgacccca cagagtttga ttacctcaaa
    25321 ttcatgtctt actacaatct aaaaactatg actaatgaag tcatggtcaa ggaagagtac
    25381 ttggcattag taaattaaaa attgcattga agatatttat atagagggga aatatatgca
    25441 aaagactgtt gaaaaaatat tattcagagt cgctggagtg acaaaatata aaaaagcagt
    25501 aaaagaagct tgcaatatga ttgctgaaga taacggaatc ccagaatatg caaagtacta
    25561 tagcaattta tcaactaagg aacttagaga agaacttgaa gaatatggtc tgaaagtttt
    25621 caaatatcaa gatttagact ttttcaatat tgagctcgtt ccagaagtag ataatagata
    25681 tgatcctaat gctataaaag ttttgatatt tgataatcat gtaggatatg ttcctgcaac
    25741 cattgctaaa acaatacgaa aatattttga taataaaaaa tataactttg taatagaggg
    25801 agagataaaa ggtggcccat ataaagaatg ggacgagtat gaagaaaaag ttgtcacaaa
    25861 taatgatttg gatgttggtt ttgaaattta ccttaccatt gttgattctt cacaaaaaga
    25921 agtaattcag agcgaatcat ctgagataat tgatgataac atttccaata aagaagttac
    25981 ggaaactgaa gctattgaaa ctaaaacaac tgaaactgaa catattgaac aaaatattgt
    26041 tagtgacagt gtcgctgata tagtaaacga gattaatctt tcagaaacat ctcctaagaa
    26101 aaaacttcct gccaataaaa tcatattttc agcactgtat cttttcttag tcttttttgg
    26161 agttgttggt attccaatcg ctccattcct tgcagtccct ttgacagctt ggagtttgta
    26221 taaactatac aaactattca gaaaataaaa aaaagcccca caatcgccct cgccaaagtt
    26281 tgattgtgaa gcttaccctt ataaaaaatc agccattaaa aaggcctctt ttctataccc
    26341 tattttacac catgaaaggg gtgatgtcaa tattctcaat gtttagacct tgtccagaag
    26401 ccgataaaca aggagaatac aatgaaatat aataaaacaa aatacccaaa tatctattac
    26461 tatgagactg ctaaaggcaa gcgttactat gtcagacgtt cttttttctt ccgaggtaaa
    26521 aaaagagaaa aaagtaaaag tggtttcaca actctccctc aagctcgtgc agccttggta
    26581 gagcttgagc aacaaatcca agaacaagaa ttaggtatca atacgaatct aacgcttgat
    26641 caatattggg atatctattc cgaaaagaga ttgtcaacag ggcgctggaa tgacacttcc
    26701 tactacctca atgacaatct ctataagaac catatcaagg caaagtttgg ttctatcctg
    26761 cttaaaaatt tggatagaaa tgagtatgaa ctatttatcg ctgaaaagtt gcagaaccat
    26821 accagataca ctgttcaaac cctcaattcc agcttcatgg cattgctgaa tgatgccgtg
    26881 aaaaatggta atctgctctc aaatcgcttg aaaggtgttt tcatcggcca gagtgatatc
    26941 cctgctgcaa acaagaaagt gactctcaaa gagttcaaga cttggatagc aaaggcagaa
    27001 gagattatgc caaaacaatt ctacgctctg acctatctga caatttttgg attgagaaga
    27061 ggagaagtct ttggattgcg tccaatggac atcactcaga acgacagcgg acgggctata
    27121 ctgcatctta gagacagtcg aagcaaccag accttaaaag ggaaaggagg gcttaaaacg
    27181 aaggattcag agcgatatgt ctgccttgat gatatcggaa cagacctgat ctattatctg
    27241 atagctgaag cttctaagat taagcgaaag ttaggaatta tcaaggaaca gcacaaggat
    27301 tatataacta tcaacgagaa aggtggtctc atcaatccaa atcagctaaa tagaaacttc
    27361 aatctagtga atgaagcaac aggattgcat gtaacacctc acatgatgcg ccacttcttc
    27421 acgactcaaa gcattattgc aggggttccg cttgaacaat taagccaggc gctggggcat
    27481 acaaaggttt atatgacgga tcgttacaat caagtagagg acgaacttgc tgaagcgaca
    27541 acagacctat ttcttagtca tattcgctaa aaaagtcccc gccaattccc cgaccaaaat
    27601 ccgaaaaata ccgaaaaata tcgaaaaatt atttttagaa tagtccccaa aagcctgaaa
    27661 tagagctaaa aaactccacc tgattcggtg gagttaaggg agattattat gaaaaagaaa
    27721 agtttaggat atttgttaca acaagttagg aggtcttctt gtaaccgtct atagtatacc
    27781 cgacctatct taaacaaatc ttaaaaatct cttaggacca aacactttct aaaatatttg
    27841 tttgttcacg accaggacct actgagaaag tagaaatacg aacgccaacc aattcactca
    27901 cacgacgaac atagttacgc gcattctcag gaagatcttc caaattgcga actccggtaa
    27961 tatcttctga ccaacctggc aactcttcat agataggctt gcaacgtttc aattgctcaa
    28021 gactagctgg atagtagtca atacgttgac cgtcaagatc ataggccaca cagattttca
    28081 cagtatccaa accgctcaaa acatcaatag agttcaaaga aaggttagta ataccagaaa
    28141 cacgacggct atgacgcatc acaactgagt caaaccaacc tacacgacgt ggacgaccag
    28201 ttgttgtacc atactcatga cccacttcac ggatacgttc tcccacttca tcaaacaact
    28261 cagttgggaa aggaccatct cctacacgac tcgtataagc tttacataca cctacaacct
    28321 tgtcaatctt gcttggaccg acaccagaac caattgtcac accaccagct acagggtttg
    28381 atgacgtaac aaatggataa gtaccttggt cgatatctag cataacacct tgtgcacctt
    28441 caaaaagcac acgtttgcca ttatcaagcg catcattcaa gataacagat gtatctatca
    28501 cgtatttctt gatttgttga ccatattcgt aatattcttc aaaaatatca tcgaaaacaa
    28561 tcgctttact gtcatacaat ttttcaaaaa gacgattctt ttcagcaagg ttacgttcta
    28621 aacgctcacg gaaaatatct ttatctaaaa gatctgcaat acgaattcca acacgagcag
    28681 ccttgtccat ataagctgga ccaattccct taattgtcgt accaatctta ttgtcgccct
    28741 tagcttcttc ttgcaagcga tccaactcga tatgataagg caaaataaca tgcgcacgat
    28801 cagaaatacg caagttatca gttgttacac cttcctcatg aagatagctc aactctttta
    28861 caagagattt aggatttaca accataccat tcccaatgac agatattttt tcagggaaga
    28921 aaatcccaga tggaatcaag tgcaacttaa atttcttacc gtcaatcaca atcgtgtgac
    28981 cagcattatc accaccttgg taacgtgcaa tcacttctgc attcgctgaa aggaagtctg
    29041 taatcttccc tttaccttca tcaccccatt gggtacctac aacaacaact gaagtcataa
    29101 tcttgtctga gccctcaggc tcttcctttc tcacatacat ggcaggactc tcacctgcaa
    29161 ttatatctta caatttatta taataaaaaa tcgccttttt atcaagaaga aacaatagaa
    29221 agatttgcta tttccaacta ttaaaaaatg atttagaaaa attactagct atttactatc
    29281 atctttccat aaaagagtaa attagttcgg aaatttacta aaattacctc aacaagaaat
    29341 aaaaccccga ttcattacca atttttcaag atacaaacga taagcaacac gataatggta
    29401 aacgataaaa tccctacgac aaccaatgcc atatctcact aaataataaa ttaaaaattt
    29461 aaaatgaaca tgttcccagt caaaattatc accaaatgta ggaccatact cttcttcaat
    29521 actatcatag aaattagcca tctgctcata aattttttgt aacataatca aatactcctt
    29581 ttctttttta taaacttatt ctaacaaaaa attttacaca ttcactatca attcctgaat
    29641 tgttaaaata ccaggctcaa caagataaaa ataggaaaag ttgacagaat agaaacaaat
    29701 ttgctaccta aatttcaaac gattaaagtt ctaaaaagac cgccttaaaa cttttgggta
    29761 aatcctatta gaactataat atttttatat aggtattatg gttctgattt tctagtataa
    29821 aaaattcaaa ctagaattct tcttagaaaa ggccgtttta agtgctatca ctgttcaaaa
    29881 atgagggtcg ctgaaacttc tatcgtcaag aagaatcatc aaattccttg tatcatcaac
    29941 caaaagattg ctcagaagct aattaaaaag acttctatga ccgacattga tcatcagttg
    30001 tctatttcaa cttcaactgt cattcgcaag atcaatgatt ttcactttga gcatgatttt
    30061 tcgcgtcttc ctgagattat gtcctaggac gttgaaacag tccggggagt gactgtttca
    30121 atcgggagat ggagatgagc tttattgctc aagactttga taagctcaat atcataactg
    30181 ttcttgagag cagaacacaa gccatcatcc gaaatcccat gaatacaagg ctatcaagcg
    30241 atactgaaag ctcattcaac aagatagtca gaaactgagt gataaacgat tttatcgccc
    30301 tacttttcgt atgcacttga ctaataaaga aattctagat aaacttttga gctattccga
    30361 agacttgaaa caccactata atctctatca gctcttgctt ttccacctct ttttcagact
    30421 ctgtttaaaa ccttcctcaa agataaagaa aaaatcgtca acggccttca attaccttat
    30481 tccaacgcca aactggaagc caccaataat ctcatcaaac ttatcaagta caatgccttt
    30541 ggttttagga actttgaaaa cttcaaaaaa gaaaggacga aatttgtcct ttctcaagct
    30601 tagcttttct tcaacccact acagttgaca aagagccact atttttggtt cattttacta
    30661 taacacgaaa tagattatta ttacataata aaaaagaggt ctaaacctct taactaaatt
    30721 actccgccag taggactcga acctacgaca tcatgattaa cagtcatgcg ctactaccaa
    30781 ctgagctatg gcggattaaa gctaagcgac ttccctatct cacagggggc aacccccaac
    30841 tacttccggc gttctagggc ttaacttctg tgttcggcat gggtacaggt gtatctccta
    30901 ggctatcgtc acttaactct gagtaatacc tactcaaaat tgaatatcta ttcaatt
//
LOCUS       DAVKJJ010000022        30952 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000022 DAVKJJ010000000
VERSION     DAVKJJ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30952
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.022"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            97..885
                     /gene="codY"
                     /locus_tag="VW144_001032"
     CDS             97..885
                     /gene="codY"
                     /locus_tag="VW144_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEU9600491.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            885..1460
                     /locus_tag="VW144_001033"
     CDS             885..1460
                     /locus_tag="VW144_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9600492.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDFFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            1542..1627
                     /locus_tag="VW144_001034"
     tRNA            1542..1627
                     /locus_tag="VW144_001034"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1576..1578,aa:Leu,seq:aag)
     gene            1910..3040
                     /gene="ugpC"
                     /locus_tag="VW144_001035"
     CDS             1910..3040
                     /gene="ugpC"
                     /locus_tag="VW144_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU9600493.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(3187..3333)
                     /locus_tag="VW144_001036"
     CDS             complement(3187..3333)
                     /locus_tag="VW144_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600494.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEY"
     gene            3541..4299
                     /locus_tag="VW144_001037"
     CDS             3541..4299
                     /locus_tag="VW144_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9600495.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKVLTGEMTLMRLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            4386..4898
                     /locus_tag="VW144_001038"
     CDS             4386..4898
                     /locus_tag="VW144_001038"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU9600496.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            5080..6024
                     /locus_tag="VW144_001039"
     CDS             5080..6024
                     /locus_tag="VW144_001039"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEU9600497.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYRYL"
     gene            6033..6710
                     /locus_tag="VW144_001040"
     CDS             6033..6710
                     /locus_tag="VW144_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEU9600498.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEISPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFIN
                     AMDLWKD"
     gene            6808..7566
                     /locus_tag="VW144_001041"
     CDS             6808..7566
                     /locus_tag="VW144_001041"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU9600499.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVVSKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            7604..9109
                     /gene="lytC"
                     /locus_tag="VW144_001042"
     CDS             7604..9109
                     /gene="lytC"
                     /locus_tag="VW144_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEU9600500.1"
                     /translation="MSVTFFIGEERLKIKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGTRYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKVIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            9605..10123
                     /locus_tag="VW144_001043"
     CDS             9605..10123
                     /locus_tag="VW144_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEU9600501.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            10253..10759
                     /locus_tag="VW144_001044"
     CDS             10253..10759
                     /locus_tag="VW144_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEU9600502.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            10759..10929
                     /locus_tag="VW144_001045"
     CDS             10759..10929
                     /locus_tag="VW144_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600503.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            10961..12193
                     /gene="clpX"
                     /locus_tag="VW144_001046"
     CDS             10961..12193
                     /gene="clpX"
                     /locus_tag="VW144_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU9600504.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            12202..12789
                     /locus_tag="VW144_001047"
     CDS             12202..12789
                     /locus_tag="VW144_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEU9600505.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            12800..13180
                     /locus_tag="VW144_001048"
     CDS             12800..13180
                     /locus_tag="VW144_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU9600506.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            13232..14122
                     /gene="rapZ"
                     /locus_tag="VW144_001049"
     CDS             13232..14122
                     /gene="rapZ"
                     /locus_tag="VW144_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU9600507.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            14119..15096
                     /locus_tag="VW144_001050"
     CDS             14119..15096
                     /locus_tag="VW144_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEU9600508.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQAILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            15093..16004
                     /gene="whiA"
                     /locus_tag="VW144_001051"
     CDS             15093..16004
                     /gene="whiA"
                     /locus_tag="VW144_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEU9600509.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(16038..17003)
                     /locus_tag="VW144_001052"
     CDS             complement(16038..17003)
                     /locus_tag="VW144_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9600510.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEFAITTSK
                     GSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSAV
                     DWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPGQLLGNGKTLDKLE
                     ITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYAI
                     GDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            17139..17996
                     /locus_tag="VW144_001053"
     CDS             17139..17996
                     /locus_tag="VW144_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEU9600511.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            17983..18762
                     /locus_tag="VW144_001054"
     CDS             17983..18762
                     /locus_tag="VW144_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEU9600512.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            18786..20138
                     /locus_tag="VW144_001055"
     CDS             18786..20138
                     /locus_tag="VW144_001055"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU9600513.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KTIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTIEEVDYYVDTITDVVRAEIGID
                     "
     gene            20212..20586
                     /locus_tag="VW144_001056"
     CDS             20212..20586
                     /locus_tag="VW144_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEU9600514.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            20588..21436
                     /locus_tag="VW144_001057"
     CDS             20588..21436
                     /locus_tag="VW144_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9600515.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            21702..22469
                     /locus_tag="VW144_001058"
     CDS             21702..22469
                     /locus_tag="VW144_001058"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU9600516.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLPDSDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            22481..23665
                     /locus_tag="VW144_001059"
     CDS             22481..23665
                     /locus_tag="VW144_001059"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU9600517.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            23662..25533
                     /locus_tag="VW144_001060"
     CDS             23662..25533
                     /locus_tag="VW144_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9600518.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMSRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQVKDRIGIVGDNGVGKSTLLNLIAGSLEPTAGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(25676..26860)
                     /gene="mntE"
                     /locus_tag="VW144_001061"
     CDS             complement(25676..26860)
                     /gene="mntE"
                     /locus_tag="VW144_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEU9600519.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            27164..29860
                     /locus_tag="VW144_001062"
     CDS             27164..29860
                     /locus_tag="VW144_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEU9600520.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVILANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEDHVLTGAELNELSDEDFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            29873..30664
                     /gene="yghU"
                     /locus_tag="VW144_001063"
     CDS             29873..30664
                     /gene="yghU"
                     /locus_tag="VW144_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEU9600521.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            30780..>30952
                     /locus_tag="VW144_001064"
     CDS             30780..>30952
                     /locus_tag="VW144_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9600522.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVII"
BASE COUNT         9108 a         5702 c         7147 g         8995 t
ORIGIN      
        1 tgtaagtcaa tgcaagtcaa taaggaattt tcagataatt attgaaaaag agacgttttt
       61 atgttataat gatagcgatt aaattcgagg tgaaaaatgg cacatttatt agaaaaaact
      121 agaaaaatta catcaatttt gaaacgctca gaggagcagt tgcaggatga gctcccttac
      181 aacgctatta cgcgtcagtt agcggatatt attcattgca atgcctgcat tatcaatagt
      241 aagggacgtc tgcttggcta ttttatgcgt tataaaacaa atacagatcg cgtagagcaa
      301 ttcttccaaa ctaagatttt cccagatgac tacgttcaag gagctaatat gatttacgaa
      361 acagaagcaa acttgcctgt tgagcatgat atgagtattt tccctgttga gagtagagat
      421 gattttccag atggcttgac gactattgca ccgattcatg tatcggggat tcgccttggt
      481 tctttgatta tttggcgtaa tgataaaaaa ttcgaagatg aggacttggt tcttgttgag
      541 attgccagta ccgttgttgg gattcagctt cttaacttcc aacgtgaaga agatgagaaa
      601 aatattcgtc gtcgtactgc tgtcaccatg gcggtcaata ccctttctta ctccgaactc
      661 cgtgctgttt cagcaatttt aggggaatta aatggaaatg aagggaagtt gactgcgtca
      721 gtgattgcag atcgtatcgg aatcactcgc tctgtgattg tcaatgctct tcgtaaactt
      781 gagtctgcgg ggattattga aagtcgctca cttggaatga agggaaccta tcttaaggtc
      841 ttgatttcag atatttttga agaagtgaag aaaagagatt actaatgaca aaggctttaa
      901 tttcgattga ttatacagaa gattttgttg ctgatagtgg gaagctgaca gcaggtgctc
      961 cagctcaggc gatttcggat gccatcagca aggtaactcg attagctttt gaacgaggag
     1021 attatatctt ctttactatt gatgctcatg aagaaaacga ttttttccat ccggaaagta
     1081 agttatttcc tcctcataat ctgattggga cgagtggacg gaatttatat ggagatttgg
     1141 ggatctttta tcaagagcat ggttcagaca gtcgtgtctt ttggatggat aaacgccatt
     1201 actcagcttt ttcagggact gacctagata ttcgtttgag agagcgtaga gtgtctaccg
     1261 ttatcttaac aggtgtcttg acggatatct gtgtcctaca tacagctata gatgcctata
     1321 atctaggata tgacatcgag attgttaaac cagctgttgc ttccatctgg cctgaaaatc
     1381 atcaatttgc cctaggtcat ttcaaaaata cactcggagc taagttagta gatgaaaatc
     1441 taaatgaact ttctgagtaa cctggttggt gaaaatgaaa aaatttgaaa aaaagtgttg
     1501 acaagcatcc caaaagttga tatactagta aagtaatcga tgcggggatg gcggaattgg
     1561 cagacgcgca ggactaagga tcctgtgacc gctttaggtc gtgagggttc aagtccctct
     1621 ctccgcatag gaaaagagtt agctaaagct agctcttttg tatttctcta aaaaattaga
     1681 atccagtcaa atgtagtgga gaatcagcga aaatatggga aaactgatcc ttgtttctgc
     1741 ttttgtcata gaagaataat aaagattatc ttcttcaaat tctccgatat tctctaaagt
     1801 tttgtgcaag ttgcacagaa cttttttatt tttttggtca tcttgccata gaaatataaa
     1861 gcgttttcat ctataatata attatcaaaa gacaaaagga gttcacctca tggtagaatt
     1921 gaatcttaaa aatatttaca aaaaatatcc aaacagcgaa cactattcag ttgaagattt
     1981 caacttgaac atcaaagata aagaatttat cgttttcgta ggaccttcag gatgtggtaa
     2041 atcaactaca ctccgtatga ttgctggtct tgaagacatt acagaaggta ctgcatctat
     2101 cgatggcgtg gttgtcaacg acgtagctcc aaaagaccgt gatatcgcca tggtattcca
     2161 aaactacgct ctttacccac acatgactgt ttatgacaac atggctttcg gtttgaaatt
     2221 gcgtaaatac agcaaagaag acattaacaa acgtgttcaa gaagcagctg aaatacttgg
     2281 attgaaagaa ttcttggaac gtaaaccagc tgacctttca ggtggtcaac gtcaacgtgt
     2341 tgccatgggg cgtgcgattg tccgtgatgc gaaagtattc ttgatggacg aacctttgtc
     2401 aaacttggat gccaaacttc gtgtatcaat gcgtgctgaa atcgctaaaa ttcaccgtcg
     2461 tatcggagct acaactatct atgtaactca cgaccaaaca gaagcgatga cacttgcaga
     2521 ccgtatcgtt attatgtcag ctactaagaa ccctgctggt acaggtacta tcggacgtgt
     2581 agaacaaatc ggtactcctc aagaagttta caaaaatcca gttaacaaat ttgttgcagg
     2641 attcatcgga agcccagcta tgaatttcat caacgtgaaa ttggttggta gcgaaattgt
     2701 ttctgacggt ttccgtttga aagtgccaga aggagcattg aaagttcttc gtgaaaaagg
     2761 ctacgaagga aaagaattga tctttggtat ccgtccagaa gacgtgaatg cagaacctgc
     2821 tttccttgaa acattcccag actgtgttgt aaaagcgact atctctgtat cagaactgct
     2881 tggttcagaa tctcaccttt actgccaagt tggtaaagac gagtttgttg caaaagttga
     2941 tgctcgtgac tacttgcaaa caggtgcaac agttgagctt ggatttgact tgaacaaagc
     3001 acacttcttc gatgtagaaa ctgaaaaaac aatctactaa aataaataaa attcaaagca
     3061 ctacaagaaa agatatctct ttatcaattg tagtggagag atatcagtta atctagggag
     3121 agaaacaaaa tgcttctctc ctttttgcta gggaagtcat attatgcatc tatattgtga
     3181 tgctctttaa tactcttcga aaatctcttc aaaccacgtc aacgtcgcct tgccgtacgt
     3241 atgattactg atttcgtcag ttttatctgc aacctcaaag atgtactttg agcagcttac
     3301 ggctagtttc ctagtttgct ctttgatttc cattgagtat tatttgtggg taccatctac
     3361 aagtgaagct atatgcgtaa actacgtgag caattgaatt cgaactagag aggtaataat
     3421 aaatttatgc tatagttatg gtgacttgta tgcttttgat tctagtttat caaataatag
     3481 attagaattg tcagataata tcattttgtg ttataatgaa gaaaaaacag aggtgttcaa
     3541 atgtcagaag caggtcataa gtttttagca aaattgggga aaaaacgctt acgtccaggt
     3601 ggaaagcgtg ccacagattg gttaattgca gaaggaggat tttcaaaaga aaagagaata
     3661 ctagaggttg cgtgtaatag gggaactaca gcaattgagt tggcacagcg ttttggttgt
     3721 aagataactg ctgttgatat ggatgctcaa gctttagaag tggctaaaaa atctgctgga
     3781 acggcaggtg ttgctcattt aatcagtttt gaaagagcaa atgcaatgaa acttccttat
     3841 caagatgcta gttttgatat tgttataaat gaagctatgc tgactatgca agccgatcaa
     3901 gctaagaaaa aatgtgtaat ggaatatcta agggtattaa aacctggagg tcttctattg
     3961 acgcatgatg tgcttcttaa ggaagctaaa gagtctatca gacaggaatt atcacaagca
     4021 attcatgtaa atgtaggtcc tttaactcaa gatggttggg aacaggtgat gatagaatca
     4081 ggttattgtg atgtgaaagt attgactggt gaaatgacat taatgagatt atcgggtatg
     4141 atttatgacg aaggtttgct aggaactttg aaaatttgtg taaatgcttg taaaaaggag
     4201 aatagaaagc agtttttaac tatgtataaa atgtttgcta agaataaaca gaaattgggc
     4261 tttattgcga tggctagtta taaatcgtca aaacgttaga taattattga agttaacttt
     4321 tccttttttc tttcttaaaa aatatgctat aatagagagt aaaaaacttt gaaagaaaga
     4381 aaaagatgaa tttaaaagat tacattgcaa caattgaaaa ttatccaaag gaaggcatta
     4441 ccttccgtga tattagtcct ttgatggctg atggaaatgc ttatagctac gctgttcgtg
     4501 aaatcgttca gtatgctact gacaagaaag tcgacatgat cgtgggacct gaagctcgtg
     4561 gatttatcgt gggttgtcca gttgcctttg agttgggaat tggttttgcg cctgttcgta
     4621 agccaggtaa attgccacgc gaagttattt ctgctgacta tgaaaaagag tacggtgtcg
     4681 ataccttgac tatgcacgcg gatgccatta agccaggtca acgtgttctt attgtagatg
     4741 accttttggc gacaggtgga actgttaagg caactatcga gatgattgaa aaacttggtg
     4801 gtgttatggc aggttgtgcc ttccttgttg aattggatga attgaacggc cgtgaaaaaa
     4861 ttggtgacta cgactacaaa gttcttatgc attattaatg aaaacagtcc ctagggctgt
     4921 tttctctaca ctaggatata aaaatagact ataactagtt agagaaaaac tataattgaa
     4981 aactatatct tcttgcagta taataaaagg actaagtgtt tgagatttgt cttcaaacat
     5041 atgcaattat tcctgaaaga gtacagttag gagagggtta tgccgattcg aattgataaa
     5101 aaattgccag ctgttgagat tttacggaca gagaatatct ttgtcatgga tgatcaacgt
     5161 gctgcccacc aagatatccg tcctttgaag attttaattt taaatctcat gccacagaaa
     5221 atggtcacag agacccagtt gttgcgccac ttggctaata cacccctaca actggatatt
     5281 gattttctct atatggagag ccaccgttct aaaacaactc gttcagagca catggagacc
     5341 ttctataaaa cttttcctga agtcaaggat gagtattttg atgggatgat catcacgggt
     5401 gctccagttg agcatttacc atttgaggaa gtggactatt gggaggaatt tagacagatg
     5461 cttgagtggt ctaagactca tgtctattcg acccttcata tctgttgggg ggctcaggct
     5521 gggctttatc tgcgctatgg tgtagaaaaa taccagatgg acagtaagct atcaggtatt
     5581 tatcctcagg acaccctaaa agagggtcac cttctattta gaggctttga tgatagctat
     5641 gtatcccctc attcacggca cacggagatt tctaaggaag aggtcttaaa caagaccaat
     5701 ctcgagattt tatcagaagg acctcaggtt ggggtttcta ttttggccag tcgtgattta
     5761 cgagaaattt atagttttgg tcatttggag tatgaccgtg ataccttggc aaaagagtat
     5821 tttcgagatc gtgatgcagg tttcgaccca catattccag aaaattactt taaagatgat
     5881 gatgttaatc aggtaccttg tctttgttgg tcttcatctg cagccctctt tttcagtaat
     5941 tgggtaaacc atgcggtcta tcaggagacg ccttttgact ggagaaagat agaagatgat
     6001 gcatctgcat ataggtattt ataagaggaa ttatgacata tttagacgct tttaaatcag
     6061 gtaccttggt tttaccgagt gccctgctct tgcattttaa ggaactcttt ccttctagcg
     6121 acgattttct ggtttggcaa tttttctatt tgcaaaatac gacaggctta gaagaaatat
     6181 cgccaagcca gattgctgaa aggattggca aggaaatttc ggatgtcaac cagtccattt
     6241 ctaatctgac ggaaagggga ctgctccagt atcgtactat cgaattaaat ggcgaaattg
     6301 aattgctctt tgatgctagt ttggccttgg aacgtttgga tgacctgttt ggagcagttc
     6361 attcaagttc agaccagcta acaccacaaa accagctcaa ggatttggtg gaaaccttcc
     6421 agcaggagtt gggacgattg ttgacgcctt ttgagattga ggatttgacc aagacactaa
     6481 aggaagatgg aaccagtgct gacttgatta aggaggctct tcgtgaagct gttttgaacg
     6541 gaaaaccaaa ctggaagtac attcaggcga ttttgagaaa ctggcgccat gaaggaatca
     6601 agagtgtggc tcaaattgag gccaagagag cagaaagaga agcaagcaat cctcagttga
     6661 cacaggtatc tgcagatttc ataaatgcca tggatctctg gaaggattaa tccatgcaag
     6721 taggcttgaa atccgagtaa gatttgcaag ctgtgtataa ttgtgataga ataaatagaa
     6781 aataaattga aaaaagaggt atgtgaaatg tcacgtaaac catttatcgc tggtaactgg
     6841 aaaatgaaca aaaatccaga agaagctaaa gcattcgttg aagcagttgt atcaaaactt
     6901 ccttcatcag atcttgttga agcaggtatc gctgctccag ctcttgattt gacaactgtt
     6961 cttgctgttg caaaaggctc aaaccttaaa gttgctgctc aaaactgcta ctttgaaaat
     7021 gcaggtgctt tcactggtga aactagccca caagttttga aagaaatcgg tactgactac
     7081 gttgttatcg gtcactcaga acgccgtgac tacttccatg aaactgacga agatatcaac
     7141 aaaaaagcaa aagcaatctt tgcgaacggt atgcttccaa tcatctgttg tggtgaatca
     7201 cttgaaactt acgaagctgg taaagctgct gaattcgtag gtgctcaagt atctgctgca
     7261 ttggctggat tgactgctga acaagttgct gcctcagtta tcgcttatga gccaatctgg
     7321 gctatcggta ctggtaaatc agcttcacaa gacgatgcac aaaaaatgtg taaagttgtt
     7381 cgtgacgttg tagctgctga ctttggtcaa gaagtcgcag acaaagttcg tgttcaatac
     7441 ggtggttctg ttaaacctga aaatgttgct tcatacatgg cttgcccaga cgttgacggt
     7501 gcccttgtag gtggtgcgtc acttgaagct gaaagcttct tggctttgct tgactttgta
     7561 aaataatcag taagtagcaa aagctaggtg gaacagcatt cagatgtctg ttacattttt
     7621 tataggagag gaaagattga aaataaaaat tggattagca agtatctgtt tactaggctt
     7681 ggcaactagt catgtcgctg caaatgaaac tgaagtagca aaaacttcgc aggatacaac
     7741 gacagcttca agtagttcag agcaaaatca gtcttctaat aaaacgcaaa cgagcgcaga
     7801 agtacagact aatgctgctg cccactggga tggggattat tatgtaaagg atgatggttc
     7861 taaagctcaa agtgaatgga tttttgacaa ctactataag gcttggtttt atattaattc
     7921 agatggtcgt tactcgcaga atgaatggca tggaaattac tacctgaaat caggtggata
     7981 tatggcccaa aacgagtgga tctatgacag taattacaag agttggtttt atctcaagtc
     8041 agatggggct tatgctcatc aagaatggca attgattgga aataagtggt actacttcaa
     8101 gaagtggggt tacatggcta aaagccaatg gcaaggaagt tatttcttga atggtcaagg
     8161 agctatgatg caaaatgaat ggctctatga tccagcctat tctgcttatt tttatctaaa
     8221 atccgatgga acttatgcta accaagagtg gcaaaaagtg ggcggcaaat ggtactattt
     8281 caagaagtgg ggctatatgg ctcggaatga gtggcaaggc aactactatt tgactggaag
     8341 tggtgccatg gcgactgacg aagtgattat ggatggtact cgctatatct ttgcggcctc
     8401 tggtgagctc aaagaaaaga aagatttgaa tgtcggctgg gttcacagag atggtaagcg
     8461 ctatttcttt aataatagag aagaacaagt gggaaccgaa catgctaaga aagtcattga
     8521 tattagtgag cacaatggtc gtatcaatga ttggaaaaag gttattgatg agaacgaggt
     8581 ggatggtgtc attgttcgtc taggttatag cggtaaagaa gacaaggaat tggcgcataa
     8641 cattaaggag ttaaaccgtc tgggaattcc ttatggtgtc tatctctata cctatgctga
     8701 aaatgagacc gatgctgaga atgacgctaa acagaccatt gaacttataa agaaatacaa
     8761 tatgaacctg tcttacccta tctattatga tgttgagaac tgggaatatg taaataagag
     8821 caagagagct ccaagtgata cagacacttg ggttaaaatc atcaacaagt acatggacac
     8881 gatgaagcag gcgggttatc aaaatgtgta tgtctatagc tatcgtagtt tattacagac
     8941 gcgtttaaaa cacccagata ttttaaaaca tgtaaactgg gtagcggcct atacgaatgc
     9001 tttagaatgg gaaaaccctt attattcagg agaaaaaggt tggcaatata cctcttctga
     9061 atacatgaaa ggaatccaag ggcgcgtaga tgtcagcgtt tggtattaag cgatgatttg
     9121 aaagagggat gtgatagtag caccctcttt ttctttgttt tatgatagtt catcctcgag
     9181 taaattcaag atcttgctcg gaaatgaagc ttatatagta gattgaatat agacaaatac
     9241 cttgtgattg gtaaaacatt ttagaaattc atttaccttt cctaatcgac ttggtttcat
     9301 cttatttcaa tctattatag tattggggaa tttcttcaaa ccacatcagc ttggtcagtt
     9361 ctacctgcga cctcaaaact tgtgctttgg tcaagctggg tttagtttcc tagtttgctg
     9421 atggatttcc attgactata agcatccaac cctctttttg tcttctaaag aattcttaaa
     9481 ttatcagtct attgcaactt ttctcatata agttctttgt cttgctattg gttttcctta
     9541 gtagtatact aaggtagtaa tcattaagaa gtggttacaa aaaataatga atgaggtaaa
     9601 gaaaatggta gaattgaaaa aagaagcagt aaaagacgta acatcattga caaaagcagc
     9661 gccagtagca ttggcaaaaa caaaggaagt cttgaaccaa gctgttgctg atttgtatgt
     9721 agctcacgtt gctttgcacc aagtgcactg gtatatgcat ggtcgtggtt tccttgtatg
     9781 gcatccaaaa atggatgagt acatggaagc tcttgacggt caattggatg aaatcagcga
     9841 acgcttgatt acactcggtg gaagcccatt ctctacattg acagagttcc ttcaaaatag
     9901 tgaaatcgaa gaagaagctg gtgaataccg taatgttgaa gaaagcttgg aacgtgttct
     9961 tgttatctac cgttacttgt cagaactttt ccaaaaaggt ttggatgtca ctgatgaaga
    10021 aggtgacgat gtgacaaacg gtatctttgc aggcgctaaa actgaaacag ataaaacaat
    10081 ttggatgctt gcagccgaac ttggacaagc acctggtttg taagaaataa ggaaacatag
    10141 ataaatctgt aggaaatttc ttacagattt tttctatttt gtaagctatt ccaaaccagt
    10201 ctttttttga gttttttgat aaaatagtac tatcagtgaa aaggatggaa gcatgactaa
    10261 gaaaatcgta gctatttggg cccaggatga agagggtgtg attggtaagg aaaatcgtct
    10321 gccttggcat ttgccagcag aattgcagca ctttaaagaa acaactctga atcatgctat
    10381 cttgatgggg cgtgtgacct ttgatgggat ggggcgtcgc ttgcttccaa aacgggaaac
    10441 cttgattttg acgcgtaatc cggaagaaaa gatagatggg gttgctactt ttcaggacgt
    10501 ccagtctgtt cttgactggt atcaggctca agaaaagaat ctctacatta tcggtgggaa
    10561 gcaaattttt caggcttttg aaccttacct tgatgaagtg attgtcactc acattcatgc
    10621 tcgggtggaa ggagatacct atttccctga agaacttgac ttgtctcttt ttgagacagt
    10681 ttcaagcaaa ttttacgcca aagatgagaa gaatccttat gattttacca tccaataccg
    10741 caagagaaag gaagtctaat ggaacggagt ttatttggtc tattcacggc ttttctgtgt
    10801 ttcatctgct ttctggcagg agcacaagct tttcgtaaaa agcgttacgg gctttctatc
    10861 ctcctttggt taaatgcctt taccaatctg gtcaatagta ttcatgcttt ttacatgacc
    10921 ttattttaga tagaatgaca gtatagaatg gaaggaaatc atgtctacaa atagaaaaaa
    10981 tgatatgatg gtttattgct cattttgtgg caaaaaccaa gaagaagtac aaaaaataat
    11041 tgctggcaac aatgctttta tttgtaatga atgcgtggag ttagctcagg aaatcattcg
    11101 agaagaattg gttgaggaag tcttggcaga cttgtctgag gtgccaaaac caattgaact
    11161 cctccatatc ttgaaccact atgtaattgg tcaagatcgt gccaagcgtg ccttggcagt
    11221 ggcggtttat aaccactaca aacgcatcaa tttccacgat acacgcgaag agtcagaaga
    11281 tgtggatttg cagaagtcaa acattttgat gattggccca actggttcag ggaaaacttt
    11341 ccttgcccag accttggcta agagcttgaa tgtacctttt gctattgcgg atgcgacagc
    11401 tctgacggag gctggttatg tgggtgagga tgtggaaaat atcctcctca aactcttgca
    11461 ggttgctgac tttaacatcg aacgtgcaga gcgtggcatt atctatgtgg atgaaattga
    11521 caagattgcc aagaagagtg agaatgtgtc tatcacacgt gatgtttctg gtgaaggggt
    11581 gcaacaagcc cttctcaaga ttatcgaggg aactgttgct agcgtaccgc ctcaaggtgg
    11641 acgcaaacat ccacaacaag agatgattca agtggataca aaaaatatcc tcttcatcgt
    11701 gggtggtgct tttgatggta ttgaagaaat tgtcaaacaa cgtctgggtg aaaaagtcat
    11761 cggatttggt caaaataata aggcgattga cgaaaacagc tcatatatgc aagaaatcat
    11821 cgctgaagac attcaaaaat ttggtattat ccctgagttg attggacgct tgcctgtttt
    11881 tgcggctctt gagcaattga ccgttgatga cttggttcgc atcttgaaag agccaagaaa
    11941 tgccttggtg aaacaatacc aaactttgct ttcttatgat gatgttgagt tggaatttga
    12001 cgacgaagcc cttcaagaga ttgctaataa agcaatcgaa cggaagacag gggcgcgtgg
    12061 acttcgctcc attatcgaag aaaccatgct agatgttatg tttgaggtgc cgagtcagga
    12121 aaatgtgaaa ttggttcgca tcactaaaga aactgtcgat ggaacggata aaccgatcct
    12181 agaaacagcc tagaggtgac tatggaactt aatacacaca atgctgaaat cttgctcagt
    12241 gcagctaata agtcccacta tccgcaggat gaactgccag agattgccct agcagggcgt
    12301 tcaaatgttg gtaaatccag ctttatcaac actatgttga accgtaagaa tctcgctcgt
    12361 acatcaggaa aacctggtaa aacccagctc ctgaactttt ttaacattga tgacaagatg
    12421 cgctttgtgg atgtgcctgg ttatggctat gctcgtgttt ctaaaaagga acgtgaaaag
    12481 tgggggtgca tgattgagga gtacttaacg actcgggaaa atctccgtgc ggttgtcagt
    12541 ctagttgacc ttcgtcatga cccgtcagca gatgatgtgc agatgtacga atttctcaag
    12601 tattatgaga ttccagtcat cattgtggcg accaaggcgg acaagattcc tcgtggtaaa
    12661 tggaacaagc atgaatcagc aatcaaaaag aaattaaact ttgacccaag tgacgatttc
    12721 attctctttt catctgtcag caaggcaggg atggatgagg cttgggatgc aatcttagaa
    12781 aaattgtgag gaaaagaaaa tggcaaaaac aattcataca gataaggccc caaaggctat
    12841 cggaccctat gttcaaggaa aaatcgttgg caaccttttg tttgctagcg gtcaagttcc
    12901 cctatcccct gaaactgggg aaattgtagg agagaatatc caagaacaga cagagcaagt
    12961 cttgaaaaac atcggtgcta ttttggcaga agcaggaaca gactttgacc atgttgtcaa
    13021 aacaacttgc ttcttgagcg atatgaacga ctttgttcct tttaatgagg tttaccaaac
    13081 ggccttcaaa gaggaattcc cagctcgttc agctgtggag gtagctcgtc ttcctcgtga
    13141 tgtaaaagtc gaaattgaag tcatcgcaga gattggataa gctagttgaa gtttggtgtt
    13201 gccaaacttc ttttgatata aggagaaaaa gatgacaaag aaacaacttc acttggtgat
    13261 tgtgacaggg atgagtggcg cagggaaaac tgtagccatt cagtccttcg aggatctagg
    13321 ttatttcacc attgataata tgccgccagc tctcttgcct aagtttttgc agctggttga
    13381 aattaaggaa gataatccta agttggcttt ggtagtggat atgcgtagcc gttctttctt
    13441 ttcagagatt caagctgttt tggatgagtt ggaaaatcaa gatggtttgg atttcaaaat
    13501 cctctttttg gatgcggctg ataaggaatt ggtcgctcgt tacaaggaaa ccagacggag
    13561 tcacccacta gcagcagacg gtcgtatttt agatggaatc aagttggaac gtgaactctt
    13621 ggcacctttg aaaaatatga gccaaaatgt ggtggatacg actgaactca ctccacgtga
    13681 actgcgcaaa acccttgcag agcagttttc agaccaagaa caagcccagt ctttccgtat
    13741 cgaagtcatg tctttcggat ttaagtatgg aatcccgatt gatgcggact tggtctttga
    13801 tgtccgtttc ttgccaaatc cctattatct accagaactg agaaaccaaa cgggtgtgga
    13861 tgaacctgtt tatgattatg tcatgaacca tcctgagtca gaagactttt atcaacattt
    13921 attggccttg attgagccga ttctgccaag ttaccaaaag gaaggtaagt ccgttttgac
    13981 cattgctatg ggatgtacgg gtggacaaca ccgtagtgtg gcatttgcta aacgcttggc
    14041 gcaggactta tccaagaatt ggtctgttaa tgaagggcat cgcgacaaag accgcagaaa
    14101 ggaaacggta aaccgttcat gagaaaacca aagataacgg tgattggtgg agggactgga
    14161 attcccgtca ttctaaaaag tctgcgggaa aaagatgtgg aaatcgcagc tatcgtgacg
    14221 gtggcagatg atggtggttc ttcaggtgaa ctccgaaaaa atatgcaaca gttgacaccg
    14281 ccaggtgatc ttcgtaatgt ccttgtggcc atgtcggata tgcctaagtt ttatgagaag
    14341 gtctttcagt atcggttctc tgaggatgcc ggagcctttg ctggccatcc attgggaaat
    14401 ctcatcattg ctggcttgtc agaaatgcag ggttcaacct ataatgccat gcagttattg
    14461 agcaaatttt tccatacaac agggaaaatt tatccttcca gtgaccatcc tttgaccctt
    14521 catgcagtct ttcaggatgg gacagaagtg gctggagaga gtcatattgt agaccatcga
    14581 ggcataattg acaatgtcta tgtgaccaat gccctaaacg atgatacgcc tctggccagc
    14641 cgtcgagtag tgcaggccat ccttgaaagt gacatgattg tcctagggcc aggttccctc
    14701 tttacctcta ttttgcccaa tatcgtgatt aaggaaattg ggcgggctct tttggaaact
    14761 aaggcagaaa ttgcctatgt ctgtaatatc atgacccaac gtggggagac ggaacacttt
    14821 acagatagcg accacgtgga agtcttgcat cgtcaccttg gtcgcccttt tatcgacact
    14881 gtcttggtga atattgaaaa agtgcctcag gaatacatga attccaaccg ttttgatgaa
    14941 tacttagtgc aagtggaaca cgattttgta ggtctttgta agcaagtttc gcgcgtgatt
    15001 tcatctaact tccttcgtct ggaaaatggc ggtgccttcc acgatggaga tttgattgtg
    15061 gacgagttga tgcgcattat acaggtgaaa aaatgagttt cacagtagca gtaaaagaag
    15121 aaatcctggg ccaacaccat ctgagctggc atgaattatc tgccattatc aaaatgtctg
    15181 gtagcatcgg tctctcgact tcgggcttga ctttgtcagt tgtgacagaa aatgccaaac
    15241 tggcccgtca cctctatgag tcctttcttc atttctatga aatcaaatct gaaattcgtc
    15301 accaccaacg tagcaatctt cgcaagaatc gcgtctatac cgtttttaca gatgaaaagg
    15361 tgcaggattt gttaagtgat ttgcacttgg cagactcttt ctttggcctg gaaacaggta
    15421 ttgatgaggc gattttatcg gatgaggaag caggtcgtgc ctatctctgt ggcgctttct
    15481 tggcaaatgg gagcattcgt gaccctgagt caggcaagta ccagttggaa atcagttctg
    15541 tttatctgga ccacgcgcaa ggaattgcct cccttctcca acagttttta ctggatgcca
    15601 aggtgcttga gcgcaagaag ggggctgtga cctatctcca gcgagcagaa gacattatgg
    15661 acttcttgat agtaatcgga gccatgcagg cacgtgatga ttttgagcgg gttaagattt
    15721 tgcgagaaac ccgtaacgac ctcaatcggg ctaataatgc cgagacagct aatatcgctc
    15781 ggacagtttc tgccagcatg aagaccatta acaatatcag taaaatcaaa gatatcatgg
    15841 gcttagaaaa tttgccagta gatttgcagg aagtggcgca actgaggatt cagcacccag
    15901 actactctat ccagcagtta gcagatagtc tcagcacccc tctgaccaaa agtggtgtca
    15961 atcacagact cagaaaaatc aataagatag ccgatgaatt ataaaaccac gaatcctatg
    16021 tgactcgtgg ttctttttta taaactggta gagtgttttg gttgtacttt ttgttcaggg
    16081 tcaatgtagt tgatagcgtt gttgacagca gttggagctt ctccgaggcc tgtcgcaatc
    16141 agatcaattt ttccgtcata gtagcagcag tcaccgatag catagatacc tgcttggctg
    16201 gattcttgtt tgctgttgac gataatcttg tgacggttga ggtcgagccc ccagttttta
    16261 aggttaccga cagaagattt gaaaccatag ttgacaaaga ggtggtctag gtcaatggtt
    16321 tcagtttcat cagatttgac ttttgtgatt tcaagtttat caagtgtttt tccatttcca
    16381 aggagttggc caggggcgaa tggtgtcttg atggttacag atgattcttg caaggcttga
    16441 acactgtgtt ccaaggcacg gaaattatct ctgcggtgaa caagggtagt tggtgcgatt
    16501 ttttcaaaag ccaaagccca atccacagcc gagtctcccc caccaagaat cgtcactttc
    16561 ttaccagcgt attgctgaat gttagaaacg tggtagtgga tattttcata gccctcaacc
    16621 ccttcaagtt ccagcggacg tggtttgaag gcaccgccac ccatagcgat gataactgtt
    16681 ttagtcaggt gacttccttt agaagttgtg atggcaaatt cttcttgttt gtcaatctca
    16741 agaaccgttt cattgagatg aataggggta tcaaatccat ttagctgttc aatcaagcgg
    16801 ttagtcaact cttctccagt caggtttggg aagcctggta cgtctaggat ttccttttca
    16861 gggtagagaa tagcaggttg tccacctagc tggggaagag agtcgatgat ttgaaccttg
    16921 gcttggcgta ggtgggcata aaaggctgca aaaagcccga caggaccacc acccacaatg
    16981 gtaatatcat agagttgaga catagtttct cctttgtttt ttctagtcag tttattttat
    17041 catatttttg ttcaatgtaa aatggactag gatagggaaa aaaatgcccg aaaatggtat
    17101 aataggacga aagtgttttg atagggagga tatcagggat gaattttcaa caattatcca
    17161 atctgcaata ttggaccagt ttgtttgcaa gtccatggac gatagctatc aatctgattg
    17221 atattttgat tgttgcttat attttatacc attttacaaa agctattgca ggaaccaaga
    17281 ttatgatttt ggtacgtgga gttttggtat ttattttagc tcaaatcctt gcaaatatga
    17341 ttggtttgac tacgatttct tggttaatca atcaaattat tacttatggg gttattgcgg
    17401 cggttgttat cttctctcca gagattcgga ctggtttgga acgtttggga agagcgacag
    17461 atttcttttc caatgcccct attagtgctg aggaacagat gattcgtgcc tttgttaagt
    17521 ctgttgaata catgagtcct cgtaaaatcg gggccttggt tgctattcag cgtgtacgta
    17581 ccttgcagga gtatatttcg acaggaattc ccttggatgc taagatttct gcagaacttc
    17641 tcattaacat ttttattccc aacactcccc tacatgacgg tgcggtgatt atcaaagaag
    17701 aacgtatcgc tgtgacgtct gcctatctgc ccttgacaaa aaacacaggg atttccaagg
    17761 aatttgggac cagacaccgg gcggctatcg gtttatcaga agtctcagat gccttgactt
    17821 ttgtcgtatc agaggaaacg ggaggaattt cgataaccta taatggaagg tttaagcaca
    17881 acctaacact tgatgaattt gaaacagaat tacgtgaaat cttacttcca aaagaggaag
    17941 tgggtcttag ttttaaagaa cgattgctag gaggatggaa acatgaaaaa aaatagttta
    18001 tatatcatat cctcactctt ttttgcttgt gtcttatttg tctatgctac ggcgacgaat
    18061 tttcaaaaca gtaccagtgc taggcaggta aaaacggaaa cctatactaa tacagtaaca
    18121 aatgtcccta ttgacatacg ctataatagt gataagtatt ttattagcgg ttttgcttca
    18181 gaagtatcag tggtcttgac tggtgcaaat cgcctatcgc tagctagtga aatgcaagaa
    18241 agtacacgta aattcaaggt tactgctgac ctaacagatg ccggtgttgg aacgattgaa
    18301 gttcctttga gcattgaaga tttacccaat gggctgaccg ctgtggcgac tccgcaaaaa
    18361 attacagtca agattggtaa gaaggctcag aaggataagg taaagattgt accagagatt
    18421 gaccctagtc aaattgatag tcgggtgcaa attgaaaatg tcatggtgtc agataaagaa
    18481 gtgtcgatta cgagtgacca agagacattg gatagaattg ataagattat cgctgtcttg
    18541 ccaactagcg aacgtataac aggtaattac agtggttcag tacctttgca ggcaatcgac
    18601 cgcaatggtg ttgtcttacc ggcagttatc actccgtttg atacaataat gaaggtgact
    18661 acaaaaccag tagcaccaag ttcaagcaca tcaaattcaa gtacaagcag ttcatcggag
    18721 acatcttcgt caacgaaagc aactagttca aaaacgaatt aaaaatagaa aaggatttta
    18781 taacaatggg taaatatttt gggactgatg gagtccgtgg agaagctaac ctagaactaa
    18841 caccagaatt agcctttaaa ctaggacgtt ttggaggcta tgttcttagt caacatgaaa
    18901 cggaagcgcc gaaagtcttt gtaggacgtg acacacgtat ttcaggggaa atgctggaat
    18961 cggccttggt ggcaggtctc ctttcagtag ggattcacgt atacaaactt ggtgtccttg
    19021 caacaccagc agtagcttac ttggttgaaa ctgaaggagc aagtgccggt gtcatgattt
    19081 ctgctagcca caacccagcc cttgataacg gaatcaagtt ctttggcggt gatggcttca
    19141 aactagatga tgaaaaagaa gcagaaattg aagccttgct agatgctgag gaagacactc
    19201 ttcctcgtcc aagtgcagaa ggcttaggaa ttttggtaga ttatccagaa ggcttgcgta
    19261 agtatgaagg ataccttgtt tcaactggaa ctcctcttga tggaatgaag gttgccttgg
    19321 atacagctaa tggagcagct tctaccagtg cccgtcaaat ctttgcagac cttggtgccc
    19381 aattgacggt tatcggggaa acaccagacg gtcttaacat caaccttaat gttggttcaa
    19441 cacatccaga agcccttcaa gaagtggtca aagaaagtgg gtcagctatt ggtttggcct
    19501 ttgatggaga cagtgaccgc ttgattgctg ttgatgagaa tggtgacatc gtcgatggtg
    19561 acaagattat gtatatcatc ggaaaatacc tttctgaaaa aggacaattg gctcaaaata
    19621 caattgtgac aactgttatg tctaaccttg gtttccacaa ggccttgaat cgcgaaggta
    19681 ttaacaaggc agttactgca gttggtgacc gctacgttgt tgaagaaatg agaaaatcag
    19741 gctacaacct tggtggtgaa cagtctggtc acgttatctt gatggattac aataccacag
    19801 gtgatggtca attatcagca gttcaattga ctaaaatcat gaaggaaact ggtaagagct
    19861 tatcagagtt ggcggcagaa gtaacgattt atccacaaaa attagttaat atccgagtgg
    19921 aaaacgtcat gaaggaaaag gccatggaag tgccagctat caagaccatc atcgagaaga
    19981 tggaagaaga aatggcgggg aacggccgta tccttgttcg tccaagtgga acagagcccc
    20041 tcttgcgtgt tatggcagaa gcgcctacaa tagaagaagt ggactactat gttgatacca
    20101 tcacagatgt agttcgtgct gaaattggga ttgactaaat cctagaaaac tagatgaaaa
    20161 taaaaaatta tggtacaata gcttataata ttgtagccga tggagaaaga catgaatctt
    20221 aaacgtgaac aagaatttgt tagtcagtat cattttgatg cgcgtaattt tgaatgggaa
    20281 aatgaaaatg gagctcctga aaccaaggtg gatgtgaatt tccaattgct ccaacatgac
    20341 caagaaaatc aagtgacttc cttgattgtc atcttgagct ttatgattgt atttgataag
    20401 tttgtcatta gtggaacaat ctcacaagta aaccatatcg atggccgtat tgtcaatgaa
    20461 ccaagtgaat tgaaccaaga agaggtggaa accttggctc gtccatgttt gaacatgctc
    20521 aatcgtttga cttacgaagt gactgaaatt gcattagact taccaggaat taatttggag
    20581 ttctaatatg aaattagctg ttttcacaga ttcctctgct tatctcagtg aagagacctt
    20641 gcaaagagaa gacttgtttg tcttggatat tcctgtcaat attgatggtg aggaatatgt
    20701 cgaaggcatc aatctgtctg ctgaggaatt ttaccaaaaa atggctcagg cttctgaatt
    20761 gcctaagacc agtcaaccaa gtattgccaa gttagatgag atcttaactt cgctcaaaga
    20821 acaaggctat acacatgcct tggggctttt cctatcttct ggaatttcag gtttttacca
    20881 aaatatccag tatatggtcg atgactatga gggcttaacc attgctttcc cagacacttt
    20941 gattacaagt gctcccctag gcatcatggt tgaaagcgtc tttaattggc gtgatcaggg
    21001 cgatgatttt gccagcattc aggataagct agccattcaa atcagccgta cgtcagcctt
    21061 tatcatggta gatgacttgg atcatttggt aaaaggtgga cgcctttcaa atggggctgc
    21121 cattttgggc aatttgctta gcattaagcc aatcctttat tttaacgatc aaggtgtgat
    21181 tgaagtttac gaaaaagttc gtactgaaaa gaaggccacc aaacgcttaa ttgaaattat
    21241 caaggaaaca acggcttcag gtcaataccg ggtcattgtc attcatggga atgctcctga
    21301 aaaggctgaa gaattgcgtc agcatttgct tgattttggc ttgggttcgg atgtttcact
    21361 tgctacattt ggtagtgtca ttggaacgca cctaggagca ggaagtattg ctctgggtta
    21421 tattccagtg atttagtagg cacttctagg ctagttaatc ttagcaattg aacacggact
    21481 acctgcctct tgaaaaaaga tgtctgtctt gcctttcgtg gaaagtcagt gccattccct
    21541 atttttcatg ggtggctaag atcctttgta ttttgataat tgaacacgcc tggaaccctg
    21601 tgtgaaaaag atagttcttc caaggagtag acacttcttg atcagaattc ctattttcac
    21661 tttgtgttct tacaggcttt gtatcttaga caggagtaga gatgagtatt cgagtaatta
    21721 ttgccggttt taagggaaag atgggtcaag ctgcttgcca gatggtcttg cctgattcag
    21781 atttggactt ggtagcagtt ttggatcctt ttgagtctga gtcagaatgg cagggtattc
    21841 ctgttttcaa ggataaggct gatttagctg gttttgaagc ggatgtctgg gtagacttta
    21901 ctactccagc tgttgcctac gaaaatacac gttttgctct tgaaaatggc tttgctccag
    21961 tagttggaac gactggtttc acgagtgaag aaattgcaga gctaaaagaa ttttctcgtg
    22021 cccaagactt gggtggcctg attgccccta actttgcctt gggtgctgtc ttactcatgc
    22081 aatttgcgac gcaggctgcc aaatatttcc caaatgtgga gattattgag ctccatcatg
    22141 acaagaaaaa ggatgctccg agtggaacag ccattaaaac agctgagttg atggcagagg
    22201 ttcgagagtc aattcagcaa ggtgcagcag atgaggaaga gctgattgct ggtgctcgtg
    22261 gtgctgactt tgatggtatg cgcatccact cagttcgttt gccaggcttg gtagctcatc
    22321 aggaagtcat ctttggcaat cagggagaag ggttgaccct ccgtcatgac tcctatgatc
    22381 gcatctcctt catgacagga gtcaatttgg gaattaaaga agttgtcaag cgtcatgagc
    22441 ttgtctatgg attagaacac ttattatgag attaacgcaa atgccttctg aatttcagaa
    22501 ggctttacca gtattagaaa aaattaaaga agcaggcttt gaggcttatt ttgttggggg
    22561 ctctgttcga gatgcccttc tccatagccc tatccacgat gtggatattg cgacgtcttc
    22621 ttatccagaa gagaccaagc agatttttcc gcgaacagcc gatatcggaa tcgagcatgg
    22681 aaccgtcttg gtcttagatg gggatgagga gtatgaggta acaacctttc ggacagagga
    22741 tgtctatgtg gactatcgca gacccagtgc ggtttccttt gtgcgctcgc tagaagaaga
    22801 cctcaaacgc cgtgatttca cagtcaacgc ctttgccttg gatgagacag gagaaatcgt
    22861 tgacttgttc catggtttag aagatttgga aaagcaagtc ttgcgagcag ttggagtggc
    22921 tagtgagcgt ttcaacgaag atgctttacg gattatgcgt ggtttccgtt ttcaggctag
    22981 tcttggtttt gcacttgagc cagaaacatt taaagctatg aagaccttga cgccgctttt
    23041 ggagaaaatt tctgtagagc gtaccttcgt cgaatttgat aaactcttgc tggctccatt
    23101 ttggagaagg ggcttggctt ccatgattga gagtcaagct tatgactatc tccctgatat
    23161 ggcatctagc caggacaagc tcaacagact gtttgatttg gagactgatt ttacttttga
    23221 atcctctgaa caagcctggg cggctttact atgggctttg gagattgaaa atgcgcagtc
    23281 atttttgaaa tcttggaaga cctcacgcca gtttgccaag caagttcagg atttgctgat
    23341 tattttggct ctgcgtgaaa atggagaatt gagcaagcga gattgttatc gctttgacat
    23401 agatttgctt ttacaggctg aaaatcttcg tcaagctcaa ggaaaagaag tcaacccaca
    23461 agccatcaca gaaaaatacc aaagcttgac tatccatgac aagaaagaga ttcagattaa
    23521 tggcgatatt ttgatcaagg aatatggcta tcagccaggc ccagacttgg gagagatttt
    23581 aacagagatt gagtttgcca ttgtcgatgg agaattggag aataatcgtg aagccatcca
    23641 tgcttacctg agggagaaaa aatgagtgat tttatcgttg aaaaactaag taaatccgtt
    23701 ggtgacaaga ccgtttttag ggatatttcc tttattatcc atgacttaga cagaattggt
    23761 ttaatcggtg tcaatgggac tggcaagacc acccttttgg acgtcctttc tggtgtttct
    23821 ggatttgatg gggatgtcag tcctttttca gctaaaaatg attaccagat tggttatttg
    23881 actcaggatc ctgattttga tgatagaaag acagttttgg atacggttct atctagtgaa
    23941 ctcaaggaaa tccagctcat tcgtgagtat gaattgatta tgctcgacta tagtgaggac
    24001 aagcaggcgc gtttggaacg tgtcatggca gagatggact ctctccaagc ttgggaaatc
    24061 gagagtcagg tcaagaccgt tcttagcaaa ttgggcattc aagacttatc tactcctgtt
    24121 ggggaattgt caggtggtct gagaagacgg gtacagttgg cacaagtctt acttggcaac
    24181 cacgaccttt tgcttttgga tgagccgacc aaccatctgg atattgcgat tattgagtgg
    24241 ctgaccctct ttttgaaaaa ttctaagaag accgtccttt ttatcactca cgatcgttat
    24301 ttcttagacg ctttgtcaac acggattttc gagttggagc gagcaggctt gacagaatat
    24361 cagggcaatt accaggacta tgttcgcctt aaggcagaac aggacgagcg cgatgcagct
    24421 ctacttcata aaaaagaaca actctacaaa caagaactgg cctggatgag cagacaaccg
    24481 caggcgcgtg cgaccaagca acaagctcgt atcaatcgtt tccatgatct gaaaaaggaa
    24541 gtttcaggca gtagtgctga gacagacttg actatgaact ttgaaaccag tcggattggg
    24601 aagaaagtca tcgagtttca ggctgtttcc tttgcctatg aaaataagcc cattttgcaa
    24661 gattttaatc tcttagttca ggttaaagac cgtattggaa ttgttgggga caatggtgtt
    24721 ggaaaatcaa ccctacttaa cctgattgca ggaagtcttg agccgacagc aggacaagtt
    24781 gtgattgggg aaactgttcg catcgcctat ttctctcaac aaattgaggg tttggatgaa
    24841 agcaagcgtg tgatcaatta cctgcaggaa gtggcagagg aggtcaagac cagtggtggt
    24901 tctacgactt ccatcgctga gttgctggag caattcctct tcccacgttc gacgcatggg
    24961 accttgattg agaagttgtc agggggtgag aaaaaacgtc tttatctcct caaactgctt
    25021 ttggaaaaac caaatgttct tcttttagac gagccaacca atgacctaga tattgcaact
    25081 ttgacagtct tagagaattt cttgcaaggt tttgcaggtc ccgttttaac agtcagtcac
    25141 gaccgctatt tcttggataa ggtagcgacc aagattctcg cttttgagga tggcaagatt
    25201 cgtcctttct ttggtcatta caccgactat cttgatgaaa aagcttttga aacagatatg
    25261 gccaatcaag tgcaaaaggc cgaaaaggaa aaagtggtca aggttcgaga agacaagaaa
    25321 cgcatgacct accaagaaaa gcaggagtgg gcaagtattg aaggtgatat tgaaaccttg
    25381 gaaaaacgta tcgctgctat tgaagaggaa atgcaggcta acggctctga ctttggtaag
    25441 ctggctactc tccaaaaaga attggatgag aaaaatgaag cactccttga aaaatacgaa
    25501 cgctatgagt atctcagtga atttgatagt taagactatt cgaaaatcaa agtgcaaact
    25561 atgtcggctt cacattgccg actcaagtac agcctgtgac cggcttccta atttgcactt
    25621 tgatttttat tgagtataaa agaatagaaa aatcccgtct catgaaagga tttctctatt
    25681 cttttttggt ttcttgatga aagatatttt gccaggtttc gtggcgacgc cagatactgg
    25741 tatggatgat accatctaac tcatagcaga tgagtttggt tttttgactg atggaagtaa
    25801 tttgaatgtc cttgatagca gaatttaact cttttttggt cttataagcc tctttatcca
    25861 tctgctctcc atcttggcga atatagacaa aatcatcagt caagagttct tctagttggt
    25921 ttccttggtc aatcaattgt tcacgcataa gcaatttttt atagacattg tctaaaattt
    25981 catcctcagg gataggtgct ggttcgatat ggacatcggt atcaaagacg ccaaaacgct
    26041 cctccagcat agactcgacc tgatccgcga tttcatggct ttcaaaaaca gacaagtcag
    26101 gattcatctc tagtgtaata tccaggtaga tgttgctacc gtaggtgcga cctctttgcg
    26161 atttgacctt gctgattttg ggaatttcca tgatagcctt ttggtagtcc tcgagcaggc
    26221 ggtcgtcaaa gccatctgaa agactaaagg aagactcgat gaagatatca taggcagtct
    26281 tcaagataaa gaaagtgatg atgatagcaa ccagtttatc cacaatcgga taattgaaac
    26341 tactagctag gatggcaatg gcggttccaa gtgaggtaac agcgtcagaa agattgtcct
    26401 tagcagctgc cttcagcgcc ttggagttgg atttcttact gaggcgagta ttgtagagat
    26461 agaccacaaa cataatcgct gcagaaatga ttcctagagt tgcaccaaga ggatcaatga
    26521 ccgtttcttc ccgactgaga atcttttgaa tggtatctct tagaacatcg aaaccgacat
    26581 agaacatgat gatagaagtg atcaagcttg ccaaatcttc aatcttccaa tgaccaaaac
    26641 ggtggtcacg gtctgcaggc tggcgcgcca tccgaatccc gattaagagg gccacatttc
    26701 caatgatgtc cgatacgtta ttaaaaccat cggccaccaa actggatgaa tgaaggagat
    26761 gaccagctgc taatttggct gcagacaaga tcaaataggt cgaaatactg ataatggctc
    26821 cacgctcagc taacttgaga tttgagatag attgcttcat tcaacttcct ttctagtcac
    26881 atagcattct accattatac tggttttcaa ggaaaaattc aaataaggca gtttttactc
    26941 tttgaaaact ccccaaaatt tggtataatg gtactactca agggagtagc tggcagaaac
    27001 ctgtgatagt gtcgtcattc cgaattttat gctgaaaagt atgctttccg gccctatctt
    27061 aaacagcgag acttgttatg attaacaggt ctctttttgt ttgtcgtgaa aagatatgac
    27121 agaaagagag tctgttttgc acacaatgtc aaggaggaga cacatgtcaa aagaacaaaa
    27181 acgccaagcg ttttatactc agagccctga agaggtcttg caggctgtgg atgcgaccga
    27241 gcaaggtttg tcatcaagtg aggcggaaaa gcgccttgcc gaatttgggc acaatgaact
    27301 cgaagaaggc gagaaacgat caatcttggt caaattcatc gagcaattta aggatttgat
    27361 gattatcatc ctagttgcgg cagcaatctt gtcagtcgtg acttctggtg gggaagatat
    27421 cgcagatgcc attatcatcc tagctgtggt tatcatcaac gctgcctttg gtgtttacca
    27481 agaaggaaaa gctgaagaag ctattgaagc cctcaaatcc atgtctagtc cagttgcccg
    27541 cgttcttcgt gatggacaca tggcagagat tgactctaaa gaattggtac caggcgatat
    27601 cgttgccctt gaagcaggtg acgtggtacc agcggaccta cgtttgatag aagccaactc
    27661 tcttaaaatt gaagaagcag ccttgacagg tgaatctgta ccagtcgaaa aagacttgtc
    27721 agtcgagctt gcgacagatg ctggtattgg tgaccgtgtc aacatggcct tccaaaactc
    27781 aaacgtaacc tatggtcgtg ggatgggtgt tgttgtcaat acaggtatgt acactgaagt
    27841 tggtcatatt gctggtatgc ttcaagatgc ggatgagact gatacaccac tcaaacaaaa
    27901 tttgaacaac ctttctaagg tcttgaccta tgctatcttg gtcattgccc ttgttacttt
    27961 tgtagtgggt gtcttcattc aagggaaaaa tccacttggt gagttgttga cttctgttgc
    28021 ccttgccgtt gcagccattc cagaaggact tcctgctatc gttaccatcg ttctttctct
    28081 tggtactcaa gttttggcca aacgacattc catcgttcgt aagttgccag cagttgaaac
    28141 acttggttca actgaaatca tcgcttctga taagactggt acgctgacta tgaacaagat
    28201 gacagtcgaa aaagtcttct acgatgcggt tctacatgac tcagctgatg atattgaact
    28261 aggtcttgaa atgccactac ttcgttcagt tatcttggcc aatgatacga aaatcgatgt
    28321 ggaaggtaac ttgattggtg acccaaccga aacagccttt atccaatatg ccttggacaa
    28381 gggctatgat gtcaaaggtt tcttagagaa atatcctcgt gtagctgaat tgccatttga
    28441 ctctgaccgt aagctcatgt caacagttca cccattgcca gatggtcgtt tccttgtagc
    28501 agtcaagggt gcgccagacc aactcttaaa acgttgtctt cttcgtgata aggctgggga
    28561 tattgctccg attgatgaga aggttacaaa tctcattcat acaaacaatt ctgaaatggc
    28621 tcatcaagcc ttgcgtgtcc ttgcaggtgc ttataagatt atcgatagta ttccagaaaa
    28681 tctcacttct gaagagcttg aaaatgattt aatttttact ggtttgattg ggatgattga
    28741 tcctgaacgt cctgaagctg ctgaggctgt tcgtgtggct aaggaagctg gaattcgtcc
    28801 aattatgatt acaggtgacc accaagacac cgcagaagct attgccaaac gtttgggaat
    28861 cattgacgca aacgatacag aagaccacgt tttaactggt gctgagctca acgaattatc
    28921 tgatgaagac tttgaaaaag ttgttggtca atactctgtt tatgcccgtg tgtctccaga
    28981 gcacaaggtt cgtatcgtca aggcttggca aaaacaaggt aaggtcgttg ccatgacagg
    29041 tgacggtgtc aatgatgccc cagctctgaa aacagcagac atcggtatcg gtatgggaat
    29101 cactggtaca gaggtttcta agggggcttc tgatatgatt cttgcagatg ataactttgc
    29161 gactattatc gtcgcagtgg aagaaggacg taaggtcttc tcaaatattc aaaagactat
    29221 tcagtaccta ctttctgcca atactgctga agtattaacc atcttcctat caaccttgtt
    29281 tggttgggac gtcttgcagc cagttcatct tttgtggatt aacttggtaa cggatacctt
    29341 cccagcaatt gcactaggtg ttgaacctgc ggaacctggt gtcatgaatc ataaaccacg
    29401 tggacgcaag gcaagcttct tctcaggtgg tgttttgagt tctatcattt atcaaggtgt
    29461 actccaagca gctcttgtta tgagtgttta tggccttgcg attgcttacc cagttcatgt
    29521 gggtgacaat catgctattc atgcagatgc cctaacgatg gcctttgcaa cccttggttt
    29581 gattcagctc ttccatgcct acaatgtcaa gtctgtctac caatccatct tgacagttgg
    29641 cccattcaag tctaagacct ttaactggtc catcttggta tcctttatcc ttctcatggc
    29701 aacaatcgtt gtagaaccgc ttgaaggaat cttccacgta accaaactag acttgtcaca
    29761 atggggaatt gttatggctg gaagcttctc aatgattatc atcgtcgaaa tcgttaagtt
    29821 tattcaacgc aaactcggtt ttgacaagaa tgcgatttaa gggaggtttc ggatgtcagc
    29881 atatcaatta ccgaccgtat ggcaggatga agctagtaat caaggagctt ttacggggtt
    29941 aaacagacca acagcaggtg cccgtttcga acaaaacttg ccaaaaggag aacaagcttt
    30001 tcagctttat tcactgggaa caccaaatgg tgtgaaggtt actatcttat tggaagaatt
    30061 actagaagct ggttttaagg aagcggctta cgacttgtat aagattgcta tcatggatgg
    30121 ggatcaattc ggctcagact ttgtgaagct caatccaaat tccaagattc cagccttatt
    30181 ggaccagtca ggtactgaaa acgtaagagt ctttgagtct gctcatattc ttctttacct
    30241 tgctgagaaa tttggagcct ttttaccaag taatcctgtg gaaaaggtag aagttttgaa
    30301 ttggctattc tggcaagcag gtgcagcacc ttttctaggt gggggatttg gacatttctt
    30361 caattatgct cctgaaaaat tggaatatcc tattaaccgt tttacgatgg aagtgaaacg
    30421 ccagttggat ttattggata aggaattggc tcagaaacct tatattgcag gcaatgacta
    30481 tacgattgca gatattgcta tctggtcttg gtatggacag ttagttcaag gaaatcttta
    30541 ccaaggttct gcaaaatttt tggatgcctc aagttatcaa aatctagtaa aatgggcaga
    30601 aaaaattgcc aatcgtccag ctgttaagcg tggattggaa gtaacttata cagaaattaa
    30661 atagcaaaaa agtcatcttc ggatggcttt ttgctacagt tgagggaaag ggtttgcagt
    30721 tatcggcttt tgtgctataa ttgctataat atagtaaaaa gcaagaggag tgtgaggaag
    30781 tggaaaagaa aattgtgaag gatatcttat ttttatctca agtgtctcag ccggcaagtc
    30841 aggaggacct ttatcttgcc agagatttgc aggatacact cttagcaaat cgtgatacct
    30901 gtgttggtct agctgccaat atgattgggg tgcagaagcg cgtgattatc tt
//
LOCUS       DAVKJJ010000023        30331 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000023 DAVKJJ010000000
VERSION     DAVKJJ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30331
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.023"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(<1..940)
                     /locus_tag="VW144_001065"
     CDS             complement(<1..940)
                     /locus_tag="VW144_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU9600523.1"
                     /translation="MLYVGIDIAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALENTGHYAFNLLNFLHEQGYKVYTYTPLLIKEFAKSLSLRKTKTNKK
                     DAHGIALKLLSDPNREQFQHDNRQVELKILARHIHRLKKKQSDWKVQYTRCLDIIFPE
                     LDKIVEKHSEYTYQLLTCYPNPQKRLEAGFDKLIEIKRLTASKIQDILSVAPRSIGTT
                     SPAREFEIIKHYKRLIDKAETCVNDLMAEFNSVITTVTGIGNRLEAVILAEIRNIHAF
                     DNPAQLQAFAGLDSSIYQSGQIDLAGRMIKRGSPHLR"
     gene            1027..1164
                     /locus_tag="VW144_001066"
     CDS             1027..1164
                     /locus_tag="VW144_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600524.1"
                     /translation="MRYQTHYQLKQKLWLEPFGNRQAIGGNELIHSGLFQVYHLGFGS
                     S"
     gene            1195..2040
                     /locus_tag="VW144_001067"
     CDS             1195..2040
                     /locus_tag="VW144_001067"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU9600525.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            complement(2187..3248)
                     /gene="dinB"
                     /locus_tag="VW144_001068"
     CDS             complement(2187..3248)
                     /gene="dinB"
                     /locus_tag="VW144_001068"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU9600526.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSKKERGVRLLGITMTGF"
     gene            3596..5920
                     /gene="pflB"
                     /locus_tag="VW144_001069"
     CDS             3596..5920
                     /gene="pflB"
                     /locus_tag="VW144_001069"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEU9600527.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            complement(6287..6505)
                     /locus_tag="VW144_001070"
     CDS             complement(6287..6505)
                     /locus_tag="VW144_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600528.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(6601..6839)
                     /locus_tag="VW144_001071"
                     /pseudo
     CDS             complement(6601..6839)
                     /locus_tag="VW144_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6832..7116)
                     /locus_tag="VW144_001072"
     CDS             complement(6832..7116)
                     /locus_tag="VW144_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600529.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            complement(7728..7898)
                     /locus_tag="VW144_001073"
     CDS             complement(7728..7898)
                     /locus_tag="VW144_001073"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9600530.1"
                     /translation="MTKQVPKFTKDTAQLYTCKWLLYNKVTKMYDHTVVNHSVREYIT
                     DSISTNTIEETG"
     gene            complement(8548..9771)
                     /locus_tag="VW144_001074"
     CDS             complement(8548..9771)
                     /locus_tag="VW144_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU9600531.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            9939..11261
                     /locus_tag="VW144_001075"
     CDS             9939..11261
                     /locus_tag="VW144_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9600532.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEAFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            11263..13149
                     /locus_tag="VW144_001076"
     CDS             11263..13149
                     /locus_tag="VW144_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEU9600533.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYINRVFKTDIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRGHDFVP
                     ELRFEDFLDKELSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEKFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYLRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGEMLWKKTRSYEELIEEYFSALYGSNWQSVVEYLEKL
                     SSYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            13317..13661
                     /locus_tag="VW144_001077"
     CDS             13317..13661
                     /locus_tag="VW144_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9600534.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            13671..15083
                     /gene="lacG"
                     /locus_tag="VW144_001078"
     CDS             13671..15083
                     /gene="lacG"
                     /locus_tag="VW144_001078"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU9600535.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGTNMWDVYLQE
                     NSPFLPDPASDFYYRYEEDITLAAEHGLQALRLSISWVRIFPDIDGEPNILAVRYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGEIGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSEDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAALNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            15153..16829
                     /locus_tag="VW144_001079"
     CDS             15153..16829
                     /locus_tag="VW144_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9600536.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMIAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGIIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSAMLAAICGYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLLT
                     PIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQFP
                     YHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLLF
                     GMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNFQ
                     LISFVFLSLMLVVDILIYLPFCRAYDRQLLVKEDIASSNDIILEEDTSEIMPGEIDVI
                     KSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIILA
                     PQVRSYYREMKVDAERLDIQIVATRGMEYIHLTKSPSKALQFVLEHYQAV"
     gene            complement(16955..18394)
                     /locus_tag="VW144_001080"
     CDS             complement(16955..18394)
                     /locus_tag="VW144_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9600537.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(18398..19747)
                     /gene="trkA"
                     /locus_tag="VW144_001081"
     CDS             complement(18398..19747)
                     /gene="trkA"
                     /locus_tag="VW144_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEU9600538.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            19936..20784
                     /locus_tag="VW144_001082"
     CDS             19936..20784
                     /locus_tag="VW144_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEU9600539.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            20799..21347
                     /locus_tag="VW144_001083"
     CDS             20799..21347
                     /locus_tag="VW144_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEU9600540.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLVGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            21354..21566
                     /locus_tag="VW144_001084"
     CDS             21354..21566
                     /locus_tag="VW144_001084"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600541.1"
                     /translation="MKIKEQTRKQVAGCSKQCFEVVDETDEVSNHTYGKAKLTWFEEI
                     FEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            21668..23350
                     /locus_tag="VW144_001085"
     CDS             21668..23350
                     /locus_tag="VW144_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9600542.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLADLDNLSIS
                     KGQVQLQTELVKETPELQSLFRLEEVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LVLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            23335..24165
                     /locus_tag="VW144_001086"
     CDS             23335..24165
                     /locus_tag="VW144_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9600543.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            24358..24861
                     /locus_tag="VW144_001087"
     CDS             24358..24861
                     /locus_tag="VW144_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEU9600544.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     regulatory      24915..25149
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            25314..25877
                     /locus_tag="VW144_001088"
     CDS             25314..25877
                     /locus_tag="VW144_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9600545.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     gene            25891..26517
                     /locus_tag="VW144_001089"
     CDS             25891..26517
                     /locus_tag="VW144_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9600546.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKNPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            26545..26934
                     /locus_tag="VW144_001090"
     CDS             26545..26934
                     /locus_tag="VW144_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEU9600547.1"
                     /translation="MKVNIADLHLTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            26939..27388
                     /locus_tag="VW144_001091"
     CDS             26939..27388
                     /locus_tag="VW144_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEU9600548.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKKIYTYNHASRRCFKSLGFVENGATEKGMSFILELV"
     gene            27516..28103
                     /locus_tag="VW144_001092"
     CDS             27516..28103
                     /locus_tag="VW144_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEU9600549.1"
                     /translation="MKLEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVTPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            28493..30100
                     /locus_tag="VW144_001093"
     CDS             28493..30100
                     /locus_tag="VW144_001093"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU9600550.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
BASE COUNT         9023 a         5289 c         6439 g         9580 t
ORIGIN      
        1 accgcagatg aggggaaccc cgtttgatca ttcttccagc taaatcaatc tgacctgact
       61 gataaataga agaatccagt ccagcgaaag cttgtaattg agcaggatta tcaaaggcat
      121 gaatatttcg aatctcggct aaaatgaccg cctctaaacg attcccaatc ccagtgaccg
      181 tcgtgatgac cgagttgaac tcagccatca agtcattgac acatgtttcc gccttgtcaa
      241 tgagcctctt gtaatgtttg atgatttcga attcacgagc aggagatgtt gttccgatag
      301 aacgaggtgc aactgagagg atatcctgaa ttttagaagc ggtcaatcgc ttaatttcta
      361 tcagcttatc aaatcctgcc tcaagccttt tctgaggatt agggtagcat gtcaagagtt
      421 ggtaggtata ttctgaatgc ttttcaacga ttttatccaa ctcaggaaag atgatatcaa
      481 gacaacgagt gtattgtact ttccaatcag actgtttttt cttgagacga tgaatatgtc
      541 tagccagtat ttttagttct acttgccgat tatcgtgttg aaattgttca cgattggggt
      601 cagaaagaag tttaagagcg atgccatgag cgtctttctt attcgtttta gtcttgcgaa
      661 gtgataatga tttggcaaat tccttgatga gcaaaggagt gtaggtgtaa actttatatc
      721 cttgttcatg caggaagttc agtagattaa aggcataatg tccggtattt tcaagagcga
      781 tgagacagtc ttggttgagc tgtcgaagag acagatctaa gagttcaaaa ccagctttat
      841 tatttgaaaa agtgagtggt ttaagaacag tttttcctgg aacattcaag gctgtaacat
      901 cgtgtttatt tttagcgata tcaatgccca cataaagcat gggagtacct ccagatatag
      961 tatttcaagt ctacttggtt atccacgaat tttttgcctt gttaccttag acgagatcaa
     1021 acgtctatgc gttatcaaac tcattaccaa ttgaaacaaa agctgtggtt agagcctttc
     1081 ggaaatcgtc aagcgattgg aggaaatgaa ctaatccaca gtggcttatt ccaagtatac
     1141 cacttgggct ttggcagtag ctaactgcgc taattataat atagggagta atctatgtat
     1201 cttattgaaa ttttaaaatc tatcttcttc gggattgttg aaggaattac ggaatggttg
     1261 ccgatttcca gtacaggtca cttgatttta gcagaggaat tcatccaata ccaaaatcaa
     1321 aatgaagcct ttatgtccat gtttaatgtc gtgattcagc ttggtgctat tttagcagtt
     1381 atggtgattt attttaacaa gctcaatcct tttaaaccga ctaaggacaa acaggaagtt
     1441 cgtaagactt ggagactatg gttgaaggtc ttgattgcta ctttaccttt acttggtgtc
     1501 tttaaatttg atgattggtt tgatacccac ttccataaca tggtttcagt tgctctcatg
     1561 ttgattatct acggggttgc cttcatctat ttggaaaagc gcaataaagc gcgtgctatc
     1621 gagccaagtg taacagagtt ggacaagctt ccttatacga ccgctttcta tatcggactc
     1681 ttccaagttc ttgctctttt accagggact agccgttcag gtgcaacgat tgtcggtggt
     1741 ttgttaaatg gaaccagtcg ttcagttgtg acagaattta ccttctatct tgggattcct
     1801 gttatgtttg gagctagtgc cttaaagatt ttcaaatttg tgaaagccgg agaactcttg
     1861 agctttgggc aattgttttt gctcttggtc gcgatgggag tagcttttgc ggtcagcatg
     1921 gtggctattc gcttcttgac cagctatgtg aaaaaacacg acttcaccct ttttggtaaa
     1981 taccgtatcg tgcttggtag tgttttgcta ctttacagtt ttgtccgttt atttgtataa
     2041 gaaaaacctt gaaggggcaa ctcttcaagg ttttatactc ttcgaaaatc tcttcaaacc
     2101 gcgtcagctt tatctgcaac ctcaaaacag tgttttgagc agcctgcggc tagcctccta
     2161 gtttgctctt tgattttcat tgagctttaa aatccagtca tggtaatccc caataggcgg
     2221 acacctcttt ctttcttgct taattcttca tagagttgca gggctatttg gcttatctga
     2281 ctagcatctt gtgttttttg agcaagactt tttcgtttgg taagagttga aaagtcctcg
     2341 tagcggattt tcaaaatgac aatttttcca gctttttctt gttgatgtag attgagagcg
     2401 actttttctg atagaagagt cagctctttt ttgatatctt cctcagcaag gagaatcttc
     2461 ccgtaggttt tctccttgcc gattgattta cggatgcgat tggatttgac tggagagttg
     2521 tgaatgccac gagcctttcg atacagatca tagcctagtc taccaaaacg gtctattagg
     2581 gttacctcag gaacttcaag taaatcagca ccagtaaaaa cgcccatttg atgaagacgt
     2641 tctactgtct tttttcctac tccatgaaat ttggaaatat ccatttgttt gagaaaatcc
     2701 tcagcctgtt caggtagaat cactgtcaaa ccatgtggtt tttgataatc actcgccatt
     2761 ttagctaaga atttgttgta agaaacgcct gcggaagcag ttagatggag ttcttgccag
     2821 atatcttttt gaatgaggcg agcaattttg accgctgact tgataccgag tttattttct
     2881 gtcacatcca aataggcttc gtcaatgctc atgggttcaa tcaaatctgt atagcgctta
     2941 aaaatagctc gaatctggag tcccacagac ttgtatttct cataattccc tgagataaag
     3001 acagcctggg gacaacgttc ataagcttcc ttggaactca tggcagaatg gacaccaaaa
     3061 gctcttgcct cataactaca ggtagaaacg actccccgtc cacctgtttg ccgagggtcg
     3121 cttccaataa tgacaggttt tcctctgagt ttaggattat ccctgatttc cactgcagca
     3181 aaaaaggcat ccatgtcaat atggatgatt tttcttgaca aatcatttaa caaaggaaaa
     3241 atcaacatgc ctagcacctt tttatactct tcgaaaatct cttcaaacca cgtcagctct
     3301 atctgcaacc tcaaaacagt gttttgagca atctgcggct agcttcctag tttgcttttc
     3361 gatttccatt gagtgttact gcttattttc ttttattata cccttttttc tgaaaaaaag
     3421 aaaaaaggac tttatttttt caaaaatata atacagtttg aaataaaata tagactgttt
     3481 tagaaaagaa agtgtaaaaa tagggaattt tcacttgttg aaatcggtta ctatatggta
     3541 tacttgtctt atgaatgtaa cagatgactg ttactagaaa aaagaggaca ttaatatggt
     3601 tgttaagaca gttgttgaag cacaagatat ttttgacaaa gcttgggaag gcttcaaagg
     3661 cgtagattgg aaagaaaaag caagtgtatc acgatttgta caagctaact acacacctta
     3721 tgatggagac gaaagcttcc ttgcaggacc aacagagcgt tcacttcaca tcaagaaaat
     3781 tgtagaagaa actaaagcac actacgaaga aactcgtttc ccaatggaca ctcgtccaac
     3841 atctatcgct gatatccctg ctggatttat cgacaaagaa aatgaagtta tcttcggtat
     3901 ccaaaacgat gaactcttca aattgaactt catgccaaaa ggtggtatcc gtatggctga
     3961 aactactttg aaagaaaatg gatacgaacc agacccagct gttcacgaaa tcttcactaa
     4021 atatgtaaca acagttaacg acggtatttt ccgtgcctac acttcaaata ttcgtcgcgc
     4081 tcgtcacgca cacactgtaa ctggtcttcc agatgcatac tcacgcggac gtatcatcgg
     4141 tgtttacgca cgtcttgctc tttacggtgc agactacttg atgcaagaaa aagtaaatga
     4201 ctggaatgca atcaaagaaa tcgatgaaga aacaatccgt cttcgtgaag aagtaaacct
     4261 tcaataccaa gcattgcaac aagttgttcg cctgggtgac ctttacgggg ttgatgttcg
     4321 caaaccagcg atgaacgtga aagaagcaat ccaatgggtt aacattgctt tcatggctgt
     4381 ctgccgtgtg attaacggtg ctgctacatc tctaggtcgt gtaccaatcg tattggacat
     4441 ctttgcagaa cgtgaccttg ctcgtggtac atttactgaa tcagaaatcc aagaattcgt
     4501 tgatgatttc gttatgaaac ttcgtacagt taaatttgct cgtacaaaag cttatgacca
     4561 attgtactca ggtgatccaa cctttatcac aacttctatg gctggtatgg gtaacgacgg
     4621 tcgtcaccgt gttactaaga tggactaccg tttcttgaac actcttgaca acatcggtaa
     4681 ctcaccagaa ccaaacttga cagttctttg gactgacaaa ttgccataca acttccgtcg
     4741 ctactgtatg cacatgagcc acaaacactc ttctatccaa tacgaaggtg taacaacaat
     4801 ggctaaagac ggatatggtg aaatgagctg tatctcatgc tgtgtgtctc cacttgatcc
     4861 agaaaatgaa gaacaacgcc acaacatcca gtacttcggt gctcgtgtaa acgttcttaa
     4921 agcccttctt actggtttga atggtggtta cgacgatgtt cacaaagact acaaagtatt
     4981 tgatatcgaa ccaatccgtg acgaagttct tgaatttgaa tcagttaaag cgaactttga
     5041 aaaatctctt gactggttga ctgacactta cgtagatgcc ttgaacatca tccactacat
     5101 gactgatagg tacaactacg aagctgttca aatggccttc ttaccaacta aacaacgtgc
     5161 caacatggga ttcggtatct gtggatttgc taacactgtt gatacattgt cagctatcaa
     5221 atacgctaca gttaaaccaa tccgtgacga agatggctac atctacgatt acgaaacaat
     5281 cggtgactac ccacgctggg gtgaagatga cccacgttca aacgaattgg cagaatggtt
     5341 gatcgaagct tacacaactc gtctacgtag ccacaaacta tacaaagacg cagaagctac
     5401 agtatcactt ttgacaatca catctaacgt tgcttactct aaacaaactg gtaactcacc
     5461 agttcacaaa ggtgtatacc tcaacgaaga tggttctgtg aacttgtcta aacttgaatt
     5521 cttctcacca ggtgctaacc catctaacaa agctaaaggt ggttggttgc aaaacttgaa
     5581 ctcactttct agccttgact ttagttatgc agctgacggt atctcattga ctacacaagt
     5641 atcacctcgc gctcttggta agactcgtga tgaacaagtt gataacttgg taacaatcct
     5701 tgatggttac ttcgaaaacg gtggacaaca cgttaacttg aacgttatgg acttgaacga
     5761 tgtttacgaa aaaatcatgt caggcgaaga cgttatcgta cgtatctctg gatactgtgt
     5821 aaacactaaa tacctcactc cagaacaaaa aactgaattg acacaacgtg tcttccacga
     5881 agttctttca atggatgacg ccttggatgc attgagctaa tcaagttctt gaataataaa
     5941 aaggaaccct cggtcaaacg actgagggtt ttgtgcttgg gatagtatga gcaattcctt
     6001 cggcgcaata tgcaatgttt ttgggaagga atagtggacc gaatctgaaa tataatagat
     6061 tgaaactaga atagtacacc tttacttcta aaacattctt agaaatcgat ttgactgtcc
     6121 tgatcgattt gtcctattct tatttcattt tactatagtc cgctgaaacg gctcaaacag
     6181 tgctagaaat tgactggaat tctctaagtg atttgttccg attttatttc atttcatcat
     6241 aaaaataaga tgactggcta attgacaatc atcttgtttg atactgttaa taaaattcgc
     6301 gaagtttatc ttgattttct ttaaaaaatt tttctaacca atccatacca taagcataac
     6361 taaaaccttc taatagaaca gagaggtcaa tagtatcaaa tcccatgtct tttaaatatt
     6421 taaaggtatc gaatggtgaa tgaaagtttt tgtatttaat aattgcacca atatcttgca
     6481 aatcctgctc tctgatactc atcatcttca ttcctaacac atactcaata gggactacta
     6541 aaacggtcag attctcaaat gagtacaagg attcgcataa acttaaggga ggttgtttgt
     6601 tcatatttgc tttgcaacat gattgttgag ccataactct tcgtggatgt taagattaaa
     6661 ctgttttcct acacgagcaa taatttcatt gattttctga ttttggtcat aaaaagcatc
     6721 aacatcttgt gtggcacgta aaccatgata ttctaagaca taaccataat tgttaaagtc
     6781 agattttcct gaattaattt cttatttagg gcttggaaga ccggtttcat tttatccatg
     6841 tttgtatttc tccatcaatt tttgaacatt ggttaactta gcaggtttat ttaatgaaat
     6901 cacatattta tgagttggag tatgctcttg aaaggcatac ttcaagctaa ttttttgaaa
     6961 acgggttagt tctttggagt ctagcaactt ttctacatct tggtttgtta aataagaata
     7021 gtcttcaaaa ataacttttc gcaaatagcg aaaccaatgg cttgcgttga attgtgattg
     7081 tttggatgcc tgtatcatat aatcgtacca atccattgtc ttcctcctat aaaatttagt
     7141 ttatattatt gtagtttata ttcaaaaatg tagtttttct taattagctc gtatctatag
     7201 gatgatactg aaaatcttct tgttgcttta aatcacaatc gaaaaattct gtttttcatc
     7261 ttagaaaaga atgtttagaa atgtaaaatg aacctttttt cttatccgaa aaccttacta
     7321 aagtatataa tttaggcagt caattctgta tgataatgtt tgtactatcg tcgagttttt
     7381 gcttgattgc ctaacgagat aaattttaga agagttttcg gtgtgatttg acaagtttct
     7441 gactggaagg atagttacac tagtattgtt tattaaaatg agcagataga gaaaatttgg
     7501 aatctatttg cttattatca atcgtttttt tagcttattt gtcttagatt ggtatagaac
     7561 tgagctatgg attaatcttc ttctatctta tcctgttcct aaatatgttg gtagaaaaat
     7621 gaagcagttg ttacgagtgt ctgtattggg aaaaattttg tctatagaat tctttaaatg
     7681 atatgaaggc taggaatgcc aaaacactga catatttcgt ttaaagtcta tccagtctct
     7741 tcaattgtat ttgtagaaat gctatcagta atatattcgc gaacactatg atttacaaca
     7801 gtgtggtcgt acatctttgt aactttatta tacaacaacc atttacaggt gtaaagttga
     7861 gcagtgtctt tagtaaattt agggacttgt tttgtcatag ttttgatacc tgtattcgtt
     7921 gtttcatgag catttgacgt ttcttttcat gtaggcattt caacaattag aatgatattt
     7981 ttccgaggat gaacttatat attgtgactt tttagagtta tgatgcctct tgatttgact
     8041 atcgctatca atttcatcca cttttacagt ttcatggttt tatgaaccga aacatctagg
     8101 tacaggtttt agcatcaatt gattgttttt gagccatatg aagttgaaac gtggagatgc
     8161 ttttcttgtg ataacgccct attcaaagag gaataaccgt ttttggagag agttcagtac
     8221 tatgtggcgc taaatactat atgtaaaaca tatattaaac aagatttaac taaggagaat
     8281 tgtaaaaaca aaaatgagaa tttcatataa agtttattct aggagtatgt atcttgaata
     8341 aaaatcgtta tttaagttca gggttatgat gaatgttttt ataataataa aatgcagcta
     8401 gaagaaaagt tattttaata taaatcactc aacatagaga gaaggcggtt tcctatatgg
     8461 aagtcgtctt gttttcatat tgtctaaata cgagatatct actacaggtt atagttgatt
     8521 taaacaggtc tatagatttc taatatatta aggtgaaata atatcttgta atacattgct
     8581 atgttctatg aaaaaagcga cgatagctaa tgcacaagct cctatacttc cacgatgttt
     8641 gttgtaaggt aaaatttcaa tttctatatt acgagtaaag ggaacgaagt ggagctggtc
     8701 ttggatttta tccctgactt catgagtgaa aggttgataa ttaagcaatt gactgttgat
     8761 atagatttta tcagcaggat tgatgaggag gagatttgca atagaagtgg ctaataaatt
     8821 gactccttta tcaatttgtt gacgtaaagc ggagtcgcct aaattataag ccgttaaaag
     8881 ggtgtctaaa tgaatgtctt tttcagtctt tacaaggctt tttagtacag ttagttggga
     8941 atttttaaat aataattggg cgtgtttgat taaccaagca tccgaaatat atgtttgtaa
     9001 acaacctttt tttccgcatt cacaatattg cccattcaat tcgacaatgg tgtgtccaat
     9061 ttctccgata taaggatttt tagaggctcc tattttttct tttgtgaaaa aggaagtgta
     9121 aatacctaat ccagcgtgta ggaaaataaa gttatcgggg gtattgtgtg gattaaaaag
     9181 gtattgtcct atagccatac aatcgatatt attttttaca ataaaaggaa aattgaatct
     9241 tttaattaca tttaataaat taaaagattc ccatatgggg ttgtttgtaa tgatacttcc
     9301 ggagtctttg ttataatgtc caggtattga taagccaaat ccaagtaatt ttgtttccaa
     9361 agggacgtac tctatagctg tttttatcat attggtaatg gtggaattga tttctgaaat
     9421 aggataatct tcattcaaaa tttctgtctc gaaaagagtt ttcccttgta agtcacatag
     9481 actgagaaag atacctagga cgtttatttc aatacctcca acaacatacg gaatattatc
     9541 tgtgatagaa atagtttttc tacgtcttcc agatccggaa aactcgctaa attcatcacc
     9601 agtttcgtat atgagggact gttcgcttag ctcttttatg atgttagtcg ttgttgctgg
     9661 agtaagtcct gtttcattaa caatatctgt acgggatgtt gctggatttc gatatagaaa
     9721 ctccagtata gtggctctat tttgtaaaaa ggctgattgt tttgtaatca tttgtttcct
     9781 cctttggttt attttatatt aaacataaga aaaaatcaat taaaattatt attctataca
     9841 aagaaaaaaa tattgtaaaa acaacaaaaa atatattgac aacgctttct gttagtggta
     9901 tatttaattt ataaaaaata aaaaaaggag ttccggtgat gaacaatatt ttagcgtttt
     9961 tagaaacaaa agtcgctccg tttggtgaaa aagttggcaa ccaacggcat ttgaaagcta
    10021 ttcgtgaagg atttatgatg gcaatgcctt tgattttagt cggctcttta tttcttattc
    10081 taatcagttg gcctcaagag gcttttacaa attggctgaa tagtgttgga ttgctaagta
    10141 tcttgacaac tatgaatcag tcaacagtag cgattatctc cttggtcgct tgtttcggta
    10201 ttgcctacag gttgtcggaa ggatatggta cagatggtcc gtcggcaggg atcatagcct
    10261 tatccagttt tgtattgatg gcacctcgtt tttcgagtat ggtttatgat aaaaatgggg
    10321 agcaggtcaa gcagttattt ggcggcgcaa taccattttc tagcctgaat gcatcttctt
    10381 tgtttatggc gattactatt ggattggtta cagcagagat ttatcgtatg tttatccagc
    10441 gcggaattac gataaaaatg ccaagtggtg tcccagatgt ggtaagtaaa tcattttcag
    10501 ctcttttacc tggctttact acttttgttt tgtgggctct ggtcttaaaa ggtcttgaag
    10561 cggcaggagt tgcaggaggt ctcaacggac tcctaggtgc aattgttgga acaccgctta
    10621 agttaattgc aggaacgctt ccaggtatga ttctatgtgt tattgtaaac tcattctttt
    10681 ggttctgtgg agttaatggg ggacaagttt taaatgcttt tgtagatcca gtttggttac
    10741 aatttactac agaaaaccaa gaagctgtgg ctgcaggaca aacactccaa cacattatta
    10801 cattaccgtt taaagattta tttgtattta ttggtggcgg tggagcgact attggtcttg
    10861 cgatttgtct cttcctattt agtaagagtc gtgcgaataa aacattaggt aagctagcta
    10921 ttataccgtc tatttttaat atcaatacag ctattctatt tacgtttcca acagttttaa
    10981 atccgattat gctgattccg tttattgcta ctcctacaat caatgcctta attacctatg
    11041 tatcaatggc tgtaggatta gtaccctaca caacaggtgt aatccttccg tggacaatgc
    11101 caccgatcat aggaggcttc cttgcgacag gagctagttg gcgaggagct ctattacaag
    11161 ttgttttgat tttggtttct gtagcaattt attatccatt cttcaaaatt gcagataaac
    11221 gcaatcttga aaaagaaaaa gctactgttg gagggaaata agatggttat cagagtattt
    11281 gatcagcaga aaaatactta ttctagcttt gccttagagg aattaagtta ctatataaat
    11341 cgggtcttta agactgacat agaacttgtc gaggagaagg aagcggatat ttttgtagga
    11401 ttagtcaata aagaggatag aaaagaccat gttcttatct cattagacaa gggtaagggg
    11461 agaattgagt ctaatacaat tgtaggttta cttattggaa tttaccgaat gtttcatgaa
    11521 tttggggttg tgtatactag accagggcgc ggacatgact ttgttccaga gttacgattt
    11581 gaagattttt tagataaaga gctatctata gatgaaacag ccagttacta tcatagggga
    11641 gtatgtatag agggagcaga ttcatttgaa aatatactag atttcattga ttggctacct
    11701 aagattggga tgaacagttt tttcattcag tttgaaaatc cctattcttt tttgaaacgt
    11761 tggtatgaac atgagtttaa tccataccta aataaagaaa aattctcaaa tgaattagta
    11821 caagaattga gtgataggtt ggataaagaa ttgcaaaaaa gaggtcttat tcatcatcgt
    11881 gttggtcatg gatggacagg tgaggtttta ggttactctt caaaatttgg ctgggaatca
    11941 ggtcttagta tttcagagga gaagaaaccc tatgtcgctg aaataaacgg gaaacgagaa
    12001 ttatttaata cggctccgat tttaaccagt ctggattttt caaatccaga tgtggcggat
    12061 aagatggtag aaattatcaa ggattatgcc aagaaaagac ctgatgttaa ctacttacat
    12121 gtatggttgt cggatgctcg taataatatt tgtgaatgcg aaaactgtag acaagaattg
    12181 gtttcggatc agtatattcg tattctcaat caattggata gggctttaac gagtgaggga
    12241 ttagatacaa agatttgttt tctgctttac catgagttgt tatgggcgcc tcagaaagaa
    12301 aaattagata atcctgaacg ctttaccatg atgtttgcac cgattacaag aacatttgaa
    12361 atgagttatg cagatgtaga ttttgacaat tccataccta cgcctaaacc ttatctgcgt
    12421 aataaaatta tacttccgaa ttctcttgag gaaaatttat cttatctttt tgagtggcaa
    12481 aaaacattta aaggagatag tttcgtatat gactatcctt tagggcgtgc tcattatggc
    12541 gatttaggct atatgaaaat tagtcaaacc atttacagag atgtatctta tctttctaac
    12601 ctacatttga acggttacat ttcgtgtcaa gaattacgtg ccggattccc tcataatttt
    12661 cctaattatg ttatggggga aatgctctgg aagaagacaa gaagttatga agaattgatt
    12721 gaagaatact tttctgcttt gtatgggtca aattggcagt ctgttgttga atatttagaa
    12781 aaattatcca gttattcctc atgtgattat tttaatgcaa ttggcagtcg tcaaaatgat
    12841 gttttagcga atcattatta tatagcttac aatctagctg ataatttttt gccaattatt
    12901 gaggaaaata tttctaagtt attaaatagt caaaaggatg aatggaaaca gctcagttat
    12961 catcgtgaat atgttgttaa gatggcgaag gctttatatc ttcaagcaac cggaaaaaca
    13021 aggcaagctc aagatgaatg gagaaatgtg ttgaattata tccgtgggca cgaattgcta
    13081 tttcaatcta atttggatgt ttatcgtgta attgaagtag caaaaaatta cgctggtttc
    13141 cacttataaa tcataagtat agaaaatgaa ccaaggtatt cagagaagat tgatcctaaa
    13201 tattatgaaa tttaaggatt tttaagatat ttagggtcga ctttctattt atatcgtagc
    13261 gaagtcattt taataatgat gtgtaaaaga tggatcaaga ttgaggagga agaaagatga
    13321 aatcaaaaga agaaataaat atgcttggtt ttacaattgt cgcttacgca ggagatgcaa
    13381 ggtcagattt gatggatgct ttggcgtttg cgagagatgg atattttgaa caggcaagag
    13441 aattggttga gtctgcaaac gactcaatag tgtctgccca tcgagaacag actaatttat
    13501 tagcggagga ggcatctgga gataattttg aagtgagttt tatcatgatt catggtcaag
    13561 ataccttgat gacaacgatg ctattgtatg atcaggtcaa gttttttatt gatgaatatg
    13621 aacgaattcg aaagattgaa gaacatattg gtttgcaatg aggattagtc atggaaaatt
    13681 tacaggttaa agccttaccg aaggagtttt tattaggagc tgctaccgct gcttatcaag
    13741 tagagggtgc aactagggta gatggcaaag gaacaaatat gtgggatgtt tatttgcaag
    13801 aaaacagtcc gttcttacca gatccagcta gtgattttta ttatcgttac gaagaggata
    13861 taactttggc ggcagaacat ggtttgcagg ctttgcgttt atctatttct tgggttcgta
    13921 tatttcctga tatagatggg gagcctaata tattagctgt tcgttattac catagagtct
    13981 ttcagtcttg cttaaaacat aatgtgattc cgtttgtttc tttacatcat tttgattcgc
    14041 ctcagaaaat gttagaaaca ggggattggt tgaatagaga gaatattgat cgtttcatac
    14101 gatatgctcg cttttgtttc caagaattta cagaagtcaa gcattggttt acaatcaatg
    14161 aactgatgtc tcttgctgca ggtcaatata taggaggtca gtttcctcca aatcatcatt
    14221 ttcaattatc tgaagcaatt caagcgaatc ataatatgtt gttggcgcat gctcttgcag
    14281 tcctcgaatt tcatcaatta ggaattgagg gagagatagg ttgtattcat gctttaaagc
    14341 caggctatcc tattgatggg caaaaagaaa atattttggc agctaaacgg tatgatgttt
    14401 ataataataa atttctatta gatggaactt ttttgggcta ctacagtgag gacacgcttt
    14461 ttcacttgaa tcaaatattg gaagctaata attctagctt tattattgaa gatggtgatt
    14521 tagaaattat gaagagagct gcagctctta atacgatgtt tgggatgaat tattatcgtt
    14581 cagaatttat tcgtgaatac aaaggtgaaa atagacaaga atttaattca acaggaataa
    14641 aaggacagtc ttcttttaaa ttaaatgctc taggtgaatt tgtaaaaaaa cctggtattc
    14701 cgacaacaga ttgggattgg aatatttatc ctcaagggtt atttgatatg ttgcttcgta
    14761 tcaaagaaga atatcctcaa catccggtca tttatttaac tgaaaatggt acagccctta
    14821 aagaagttaa gccagagggc gagaatgata ttattgatga cagtaagaga atccgttata
    14881 ttgagcaaca tttacacaaa gttttagagg ctcgagatag aggagtcaat attcaaggct
    14941 attttatatg gtctttgcaa gatcaatttt cttgggcgaa tggctacaat aagcgatatg
    15001 gtcttttctt tgttgattat gaaacacaga agagatatat taagaaaagt gctctttggg
    15061 taaaagggct aaaacggaat taaggttagc gatttgactg atgtttaata tattttaact
    15121 atgagcttaa attttttaca ggaggagttt ttatggataa gttagtcgct gccattgaaa
    15181 agcaacaagg gaaatttgaa aaaatttcta ctaataatta tatgatagct attaaagatg
    15241 gatttattgc tactatgcct ttaattatgt tttcaagctt tttgatgatt attattatga
    15301 ttcctaaaaa tttcggagta gagttaccga gtccagctat tgcctggatg aggaaagtgt
    15361 atatgctaac catgggagtt ttgggtatta ttgtttcagg gacagttgca aagtcattgg
    15421 ctggaaatgt taatagaaaa atgcctcctg gaaaggtaat aaatgatact tctgcaatgt
    15481 tggcggccat atgtggctat cttgtattaa ctgtaacgct tgtagttgat gagaaaacgg
    15541 gatctacaag tttgtcgaca aactatttag gatctcaagg attgataact tcgtttgtca
    15601 gtgcctttat tactgtaaat gtttaccgat tctgtattaa gcgagacatt actattcatt
    15661 tacctaaagt tcctggggct atatcacaag cttttagaga tatttttcct ttttcttttg
    15721 ttttacttat tagtggtttg ttagatattg tatctcggtt tagtttagat gttccttttg
    15781 cccaagtatt tcaacaacta ttgactccta tttttaaggg ggcagaatca tatcctgcta
    15841 tgatgttgat ttggtttatg tgtgctttgc tttggtttgt tggaattcat ggaccatcta
    15901 ttgtcttacc tgctgttaca gctttacaac tgagcaatat ggaagagaat gctcaacttc
    15961 ttgcaaatgg gcagttccct tatcattctt taacacctaa tttcgggaat tatatcgctg
    16021 ctattggagg aacgggggct acctttgttg taccatttat tttgattttc tttatgcggt
    16081 ctaaacaatt aaaatcggta ggtaaagcta caattactcc tgttttattt gcggtaaatg
    16141 aacctcttct atttggtatg cctgttattt tgaatcccta tctttttgtc ccttttttga
    16201 tgactccacc agtgaatgta tttctaggaa aggtatttat tgatttcttt ggaatgaatg
    16261 gattttatat ccagttacct tgggcctttc ctggtccctt gggattgtta attggaacga
    16321 attttcaact tatctccttt gtatttttat ctttgatgtt agttgtcgac atattgattt
    16381 atttgccatt ctgtagagcg tatgatagac agttactggt gaaagaagat attgcaagct
    16441 caaatgatat tattttagag gaggatacaa gtgaaataat gcctggtgag atagatgtaa
    16501 taaaaagtaa ggagttgaaa gtactggttc tttgtgcagg gtctggaaca agtgcgcaat
    16561 tagccaatgc aattaacgag ggcgctcaat tagcagaagt tagagtgatt gcgaattcag
    16621 gagcgtacgg agctcattat gatattatgg gtgtttatga tttaattatt ctggccccac
    16681 aagttcggag ttattataga gagatgaagg tggatgcaga aagattagat attcagatag
    16741 ttgctaccag aggaatggaa tatattcatt taacaaagag tccaagtaaa gccttacaat
    16801 ttgtattgga gcattaccaa gctgtgtagt aagtttttcc atcttttatt tgagtaaaga
    16861 ttttgcttac agataggctt ggatttaaaa acgttccccc ttttttaata taagaatccc
    16921 tctttcacaa ttgtaaaaag agggattttg tattttatct cttagaccaa gttctcttca
    16981 taaagagaag gaggattggg taaatctcca agcgccctgc aatcattgca aaggatagga
    17041 gaatttttga gatgggacta aagattgaga aactagaagt ggttcctaga ataggcccga
    17101 tattattgaa acagctaaag acagcgctgg tcacgaccag aaaatcattg ctatctaggc
    17161 tgacaataaa gataagcgct agcaaaatca tagcatagat gacaaagtac ttgagaatct
    17221 tatgctgggt atctttgtca atcaccgttt tattaacatg gagggtcaaa acacggtggg
    17281 gcgataggat tgacaaaatt tggtttttgg caatttttga aaggatgagg cctcgaataa
    17341 tcttgagtcc acctgcagtt gatccagcag agccaccgat tgccatgagg aaaaggagga
    17401 taaactggga gaagaggggc cagttggtaa tatctccata tccaaaacca gttgttgtaa
    17461 tgatgttgga aacctggaag aaggtcattt caaagctctt tgaaaaccct gggtagaggt
    17521 agagggtgtt gaggctaatc aagcctgtag aaaccagtac aatgaccaag taagccctaa
    17581 gctcttcatc tccaaagaag gccttgatgc gacggagcat gaggtagtag tagaggttga
    17641 aatttactcc aaaaaccaga actccgatac tgaccagata ggtaatcagt gagctgccat
    17701 agtgggcaat tccgtcgtta tagacggtaa agcctccagt tcccgctgtc cccatagcaa
    17761 taacaaaact atcgtagaga ggcataccgg ctagataata gatgatgaca aagagggaga
    17821 agagagctag ataaaggaga tagagaatct gggcagtgtt ttttagtttg gatacaacct
    17881 tgccaaaaac aggacctgga acctcagcct tcatcacctc taggtggcta tttttggcat
    17941 tgtccataat agcaagtgca aaaacaagca ctcccatccc tccaatcaag tgggtaaaac
    18001 ttcgccagaa gaggagggaa cggctgagaa ccgaaacgtc gttcaaaata cttgctccag
    18061 tagttgtaaa tccagaacta atttcaaaaa aggcatcaat aaggctgggg atttgcccag
    18121 aaaagacaaa ggggagacca ccaaagaaag accaaaggat ccaacagagg gcaacgatca
    18181 agactccctc cttggcataa atccgttgat tttttggctt ctgtaaactc cctgaaccgc
    18241 ctaacaatac gagaatccct atggtcgaaa agagggctgt aaagacttgg ctcgattcac
    18301 ggtaatagac agcaatcgca acaggaacca aaagaagaac agcttcaatc aaaagtaatt
    18361 ttgaaaggag gtaacgaatc atacttttat tcatttctta cctcgcgatc aagtcataaa
    18421 tcttggtgat gtttggcaac aaggttgtta ctaggagctt gtctccaact tccaacatat
    18481 cctccccagt tgggaaaata gtcttgccct ttcgaataat ggctgcaata agaacccctt
    18541 tttttaattt cagttgagaa agaggtttgg cagtcatttt attggcttcc ttgatatgga
    18601 attgcagggt ttcgatttgg ccattggcta gatggtgcat ggcttgaagg tctgaatact
    18661 gggcattaac tcgaccacga ataaagtgca taatcgtatc tacagcgatg cttttaggtg
    18721 tgatgatact tgaaaaatca ggcgcattga taatctcgag gagactggta cgattgacct
    18781 tagtaatatt tttctgtaca cctaccctgt caaggaacat agatgtaatc agattttcct
    18841 catcgacacc tgttagagtc gcaacggcat catagtgttg agcactttct tccagcagga
    18901 tatcttttgc ggttccatct ccttgaacga tgtagagatt tgggaatttc tcgctaaaga
    18961 agctggcgat ttcaggattg atttcaatga cttttgtatc gatacgacta tctttgagaa
    19021 taccaagtag ataataggca attctacctg ccccaacgat gagaaggctc ttcacggcgc
    19081 gtgatttaaa ataattatgg aagagtatca tatcgacacg gttaccagtg acaaagattc
    19141 tatctttatc ctgtacagtc atgtcaccgc ttggaatgat aatttgatga tccctctcta
    19201 tcgcacagac aatgacatta ccaaattttt tacgaaaatc agaaatgggc atttggcaaa
    19261 gaccgctggt ggacttgacg acaaattcca tgaggctaac gcgtccacca gcaaagcgtt
    19321 cgacagacag ggcgttgggg aagtcaatga tattcgcgat agcgcgggca gccaagagct
    19381 caggattaac gataagagaa aaaccgagaa tattcttttc cttgaaataa gagttagaat
    19441 attcagggtt ccgcacccga acgatagttt ctttagctcc cattttcttg gctagaactg
    19501 ctgcaatcat gttgacttca tcgtgctcag tcagggcgat aaagatatca caatcttgga
    19561 cgctggcttg ctcaagaatg gcaaaatcgg ccccgttacc aaggatacca atgatatcaa
    19621 agcgactgac aatatgattg agaacagctt cgtcttgctc aatcagcaaa acatcatgct
    19681 tttctgcaac caaggagcga cagagggcaa aaccaacttt tccccctccg acaaggataa
    19741 ttttcataat aaaacctact ttttcatgat gtaactatca tacccttttt caagaaaaaa
    19801 tgcacctact agctaataac cagagttttt agtaggaaat ttgctataag ttaaaactat
    19861 accctaacca attgaaatag ctattagcga ctttctctga aatatggtat gataaaggat
    19921 atacaaggag ataaaatgaa taataattta ctggtattac aatcagactt tggtctggtt
    19981 gatggtgcgg tatcggctat gattggagtg gctttagaag agtctccaac cttaaaaatc
    20041 catcacttga cgcacgatat cacgccttat aatatttttg aggggagcta tcgtctcttt
    20101 cagacggtgg attactggcc tgagggaacg acgtttgtat cggttgtcga tccaggtgtc
    20161 ggttcgaaac gtaagagtgt agttgccaag actgcaaaaa atcaatacat tgtcacgcca
    20221 gataatggga cgctttcctt tatcaagaaa cacgttggca ttgtagccat tcgtgagatt
    20281 tctgaggtgg ccaataggcg tcaaaacaca gagcattctt ataccttcca cggtcgtgat
    20341 gtctatgcct atactggtgc taaactggcc agtggtcaca ttacttttga ggaagtaggg
    20401 ccagagctca gtgtggaaca gattgtagag cttccagtcg tagcgaccat catagaagat
    20461 catctggtga agggagccat tgatattctg gatgtgcgtt tcggttcgct ttggacctct
    20521 atcacacggg aagaatttta caagttggaa ccagaatttg gtgatcgttt tgaagtgacc
    20581 atctatcatg ctgatatgct ggtctatcaa aatcaggttg tctatggcaa atcatttgca
    20641 gatgtgagaa ttgggcaacc cattctttac atcaactctc tctatcgttt aggtttggct
    20701 atcaaccaag gttcctttgc caaggcctat aatgtaggtg tcggttcatc ttggaccatt
    20761 gaaataaaga aaatagaagg ataaaatagg agaatagtat ggaaatcaaa tttacaatta
    20821 aacaagttgt tgctgtcgga attggcgctg ccctctttgt cgtcatcggg atgatcaaca
    20881 ttccaacccc tgttccaaat acaagcatcc agcttcagta tgcggtacaa gcgctacttt
    20941 ctattatttt tggaccgatt atcggtttgc ttgttgggtt gattggtcat gcaatcaagg
    21001 actctcttgt tggttatggt ctatggtgga cttggattat cgctagcgga ctctttggtt
    21061 tagttgtggg actttttaga aagtacgttc gcgtgatcaa tggtgttttt gactggaaag
    21121 atattcttat ttttaacctc attcaactac ttgcaaatgc ccttgtttgg ggtgtcttgg
    21181 caccacttgg agatgttgtg atttatcaag aagcggcaga aaaagtattt gcacaaggaa
    21241 ttgttgcggg aattgccaat ggtgtatctg tagctattgc aggaactctt ctcttacttg
    21301 cctatgcagg aactcaaact cgtgcaggaa gtttgaaaaa ggactaatac tcaatgaaaa
    21361 tcaaagagca aactaggaag caagtcgcag gttgctcaaa acagtgtttt gaggttgtag
    21421 atgaaactga cgaagtcagt aaccatacct acggcaaggc gaagctgacg tggtttgaag
    21481 agattttcga agagtataaa atgatgggga aggctgggca actagtcttt ttcgatgttt
    21541 atagattagt taggcaagta agctaggata ggaatctttt tggcgctaag attttaagag
    21601 aagagactgc aagaagggct tgatttatga taaaatagaa gtctaagaag cgaatgaaga
    21661 gagtaagatg aaagaagcta taattgagtg gaaggatttc tctttccggt atgaaacaca
    21721 acaagaaccg accttgcaag ggatagactt gaccatttac aagggagaga aagtcttaat
    21781 tgttggacca tctggatcag gtaaatctac cttgggtcag tgtttgaatg ggattattcc
    21841 caatatttac aagggtcaga catatggaga atttttgata aagggtcaaa cagcctttga
    21901 tatgagcatc tatgataagt ctcatctggt tagcacagtt ttgcaggata cagatgggca
    21961 gtttattggc ttgtctgtgg cagaagattt ggcgtttgct ctggaaaatg atgtgacagc
    22021 cctagatgag atgaaaggtc gtgtttataa atgggctgaa aagctggacc ttcttccttt
    22081 actggatcag cgccctcagg atttgtcagg tggacaaaag cagcgagtca gtctggctgg
    22141 tgtcttgatt gatgaaagtc cgattctctt gtttgatgag ccactcgcca atctagatcc
    22201 caagtcaggt caggatatta tcgaattgat tgaccagatt cataaggaag aggggacgac
    22261 gactctgatt atcgagcacc gtttggagga cgttctgcat cgccctgtgg atcggattgt
    22321 cttgataaac gatggtcgta tcctttttaa tgggagccct gaccagttgc ttgcgactga
    22381 tttattgact caaaatggaa ttcgagaacc cctttatcta acgactctcc gtcaattagg
    22441 tgtggactta gtcaaggaag aacaattagc agatctggat aacttgtcta tctcaaaagg
    22501 gcaagtccag ttacagacgg aactggtaaa agaaacccca gaattgcagt cactctttag
    22561 actagaggaa gtatcttttt cttatgatga tagaccgatt ttaaaatccc tacatttaga
    22621 tattaaaaag ggtgaaaaga ttgctattgt cggaaaaaat ggagcaggga aatcaactct
    22681 agccaaggct ataagtagct ttattcagac ggaaggacgc tatctttggg aaaaacagga
    22741 tataaaaggc gattctgttg cagagcgggc ggaacgagta ggctatgtgc tacaaaatcc
    22801 taatcaaatg atttcaacca atatgatttt tgatgaggtg gctctagtgc tccgtttgcg
    22861 aggtgtggat gagaaggaaa ttgaaacgag agtctatgaa accttgaaaa tctgtggact
    22921 ttatgaattc cgtaattggc ctatttctgc cctgtcattt ggtcagaaaa aacgtgtcac
    22981 cattgcttca attttggtct taggagctga aattattctc ctagatgaac cgactgcagg
    23041 tcaagatcag aagaactata ctgagattat ggaatttctc gaagagttac atcaaaaagg
    23101 gcataccatt gtcatgatta cccatgatat gcaattgatg ctggattatt cagaccgggt
    23161 ccttgtcatg gtggatggag aattgattgc cgatactgtt ccagccagtc tgttgagcga
    23221 tcctgagctg ttagtaaaag ccaatctaaa agaaacctcc atctttaact tggctaagaa
    23281 actagatgtg gatccactgg atttaacggc attttacaaa gaaaggagag agggatgcaa
    23341 gctaaattaa tcggttacca gcatagagat actgtgattc atcgcttgtc aggagctggg
    23401 aaacttctct ttttcattct ggtatcattg gcggccatga ttagctatga taccagaatg
    23461 ctcttgctga ttgccatctt ttcagtcttt ctcctctatt tatcagaaat tcgctttaaa
    23521 gatgtttcct ttgtagccgt ttttgcgacg gtatttgccg ttttaaacgt tttgatggtc
    23581 tatctctttt ctcccgagta tggggttgga ctttatggag agagaagtgt gatttggcag
    23641 ggaatcggtg tctacactct aaccagccag gagctctttt atctgctaaa tttggccatt
    23701 aagtatcttt gcaccattcc tctggctatt atctttttga tgacaaccca tcctagtcag
    23761 tttgcttcca gtttaaatca aattggtgtg ccctataaga ttgcttattc tgtcagcctg
    23821 accttgcgct atattccaga tttgcaggaa gaattcttta ctatcaagat gtctcaggag
    23881 gcgcgtggga tggaattatc caagaaagct tctcttatgc aacgaatcaa aggcaatctg
    23941 ctcattatta cgcccttgat ttttagctcg ctagaacgca ttgataccat tgcgactgcc
    24001 atggagcttc gccgctttgg gaaagagaaa aaacgcacat ggtatagtta tcaggccttg
    24061 aaaaaaggag actatattac cttgctcttg gcaaccttgt ttttagtagt tagtttacta
    24121 cttatcttgc agaatcaggg acgattttac aacccttgga aatagcttga aaaaattgaa
    24181 aaaatcaagt cgtttctatt gacaatgatt ctgaaagtgt tatactaaga aagtagtttc
    24241 gctgatttac ttcaaacctg ttgtgaggta agttaacgat gccttaacca cgctgtttgc
    24301 tgagcttgac tccgggcagt gtggctattt ttttgcaatg gtgaaaggaa gcaagtcatg
    24361 acaaatcaca ttgtattatt tgaacctcaa attccacaaa atacgggcaa tatcgcgcgt
    24421 acttgcgctg cgaccaattc tcccctccac atcatcaagc ctatgggctt tcctattgat
    24481 gaccgcaaga tgaagcgggc tggattggat tactgggata agctagagat ttatttttac
    24541 gagagtttgg aagatttcat atctcagatg aagggaaaac tctatctgat ttcgaaattc
    24601 gcggaaaaag tctattctga ggtggattta tcgactgacg aggaccatta ttttctcttt
    24661 ggacgtgaag acaagggctt acctgaagat tttatgcgag aacatcctga gaaggctctc
    24721 cgtattccca tgaatgatga acatgtccgc agtctcaatg tgtctaatac cgtctgcatg
    24781 attgtctatg aggctcttcg ccagcagaac tttgcaggtc ttgagctcgt ccatacctat
    24841 gaagtggata aattgaaata aaaaatgaaa atgaacaaaa tgcttgcgct tgcaagcgtt
    24901 ttttgttatg ataaaagagt cttcagggca gggtgtgatt cccgaccggc ggtgacttta
    24961 actaggaaat gatcttttcc ttttatactt tgttgacaag ctttgcctaa ccagaagtta
    25021 tgcctacagc ttgtcgccta gtctaaaaga aaaatctcta tttccttctc tttaaagaag
    25081 tccgcgagcg caagctgatg tggtgagatt ccacaaccga cagtatagtc tggatgggag
    25141 aagacgaaag aatagctttg tctgttttga tagatttata gccggattgt aaccacttgc
    25201 tttgtttttc tttaatagag taagagggaa cttttgggat ataaaaagtg agaatagata
    25261 gaggaatcct ttccaacttc ttctgatttt atagaaaatt ggaggaacct gttatgacaa
    25321 atacacgtcg actttcgacc attgcaattt tatcagccat ctcatttgtg ctgatgtact
    25381 ttgactttcc gcttttacca gcggcatctt tcctcaagat cgaatttagt atcttgccag
    25441 tccttgtggg tctggtggtc atggatttgc ctgctgctct aggagtgctc ttgcttcgct
    25501 cactcttgaa attgcttctt aacagccaag gagtgaatac ttacattggt ttgccgatga
    25561 atatcgtagc tttgggagtt tttgtcatcg tatttgcttt gatttggaaa aaggaacgga
    25621 caacccttcg tttcctacta ggctctctag ctggaactgt tggtttaacc ttggccatgt
    25681 tggttctcaa ctatgtttat gctgttcctt tgtacgctaa gtttgctaac tttgatattg
    25741 gaaaaatttt gggactttcc aactacctaa tgaccatggt attacctttt aacttgattg
    25801 agggtgtaat cttttccgtt tcattctggt tgttgtacgt ccttttgaaa ccaaccttaa
    25861 aacactatga aagataaaca aacattttta atgaagggca gttttgccct tttacttttc
    25921 gttattcttg gctatatggt caaattttac cctgaaatgt tggtcaattt tgaccaatcg
    25981 attcagactg ccattcgagg agacttacca gattatttga ctattctttt tcgagccctc
    26041 acacgtctga ttgatatccc agtgattatc acttgggttg tcattacagc ttttgtcttt
    26101 tatcgtaagc gatggaagat agaaagtttc ttcatgctgg gaaatctggc tttggcaggt
    26161 cttttaatcg tgacctttaa aaatatctac cagcgcccac gaccagctat cttacatctg
    26221 gtggaagaga agggattttc cttcccaagt gggcattctc tggctgtaac tttgatggta
    26281 ggtactctga ttgtcattct cagtcaacga attaaaaatc cagtctggag aaaaatcgta
    26341 caaatcgtcc ttggcctcta cctagtcagt gtgctggtat caagagtcta tctgggagtt
    26401 cattacccat cagacgtcct tgccagtctc tgtgtgggct tgggagtcct gtttatcgag
    26461 tttcccttct atgacaagct ccgcttccaa tggcgattta aaggcaaaca gaagtgagta
    26521 tcgaattccc ttgaggagaa agaaatgaaa gtcaatatag cagatcttca tctgactcaa
    26581 ctatatttat cagaaaagaa gttgcaagat attcagatgc tttaccagtc ggcagaaaca
    26641 atccaagtcg atccaatcag tattcttgcc tttggagatt gtttgctgat tacagatggg
    26701 catcacaggg cttatcaggc tttattggca ggtcgggata cgatttctgc tgagtgggat
    26761 agagatggtg gtgatgaact atatcatctc tatgcgcaag cttgcgagga aagaaagatt
    26821 tactctgttt ttgatttaga agatcgtatc ctagctcaag atgggtatga agcaaaatgg
    26881 tataactggt gtgatggttt taatcaagca gcaactcttt tgttgaaaag gtagtgcatt
    26941 gtgttttgtt ccttattata aggtcaatca ttgtgaggaa gcttttgctt ggtatcagga
    27001 tgtgaacttg gtttacctcg tagatggggt gaagcttcct tacagtcaag cgaccttgga
    27061 agccatgtat tcctatttgg atcggcatgg tgagcttttt tggattgaag tcaaggagaa
    27121 gggagaatgg tttccaattg gggatattac actatctcag gataatctcc ccattgtgat
    27181 tgggaattcc gcttaccaac atcgaggact tggaaaaaag attctaagtg ctttgattga
    27241 attggctcga gtaaaaggat ggaaagaatt gagagtcaag aaaatctaca cctacaatca
    27301 tgcttctagg aggtgtttca agtcgcttgg atttgtggaa aatggagcaa cagaaaaagg
    27361 aatgagtttt atattggaat tagtctaatc ctgccacttt ctccccctta acctttgact
    27421 attccgcaaa attatcgtaa aataaagagt gaatgataaa atgaggtcag agtctgttcg
    27481 ctctggcgat agtagtataa atgaggagaa acgctttgaa attagaagta tttgctgggc
    27541 aagaaaaaag tgaactatct atgattgagg tagcgcgtgc tatcttggaa cttcgtggtc
    27601 gcgatcatga gatgcatttt agcgatcttg taaacgaaat tcaaaactac cttggaacat
    27661 caaacagcga tatccgcgaa gctttgcctt tgttctacac agagttgaac tttgacggta
    27721 gcttcatctc acttggggac aacaaatggg gtcttcgttc atggtatggt gtggacgaaa
    27781 tcgacgaaga aatcatcgct cttgaagaaa atgacgacga tgaagtaaca ccaaaagcta
    27841 agaaaaaacg tgtcaatgcc tttatggatg gtgattcaga tgccattgac tacaatgcag
    27901 atgatccaga agacgaagat gcatacgaag cagatccagc tctttcatac gatgatgaaa
    27961 atccagatga tgaaaaaaat gaagtggaag cttatgatgc agaaatcaac gaaatcgctc
    28021 cagatgactt gggagaagat gtggatctca acgaagacga cgacgagttt tcagacgacg
    28081 acgctgaaac cagcgaggaa taagagttag ctattgacaa ttatcctatt tttaggtatc
    28141 atattgtttg ggcacctctt ttagaggtcg gggctcccta gttcttaggg agctattttt
    28201 gttttttcaa gaagttatct tcttgtattt tatactcaat gaaaatcaaa gagcaagcta
    28261 ggaaactagc cgtaggctgc tcaaaacact gttttgaggt tgtagataag actgacaaag
    28321 tcaggaacac atatctacgg caaggcgacg ttgacgcggt ttgaagagat tttcgaagag
    28381 tattagttgt gaatctggtg cagtcgtccc agattattct tattagtagg gtcttgtttt
    28441 ctatatcccc tcgtagttaa caagaccttg agcattttag aaagaggaat ctatgtctac
    28501 gaaatatatt tttgtaactg gtggtgtggt atcgtccatt gggaaaggga ttgtggcagc
    28561 gagtctaggc cgtctcttga aaaatcgtgg tctcaaagta accattcaaa agtttgaccc
    28621 ttatatcaat attgatccgg gaaccatgag tccttaccag cacggggaag tttttgtgac
    28681 agatgacgga gctgagacag atttggactt gggtcactat gaacgtttca tcgatatcaa
    28741 tctcaacaaa tattccaacg tgacaactgg gaaaatttac agtgaagttc ttcgtaaaga
    28801 acgccgtgga gaataccttg gggcaactgt tcaagtcatt cctcatatca cagatgcttt
    28861 gaaagaaaaa atcaagcgtg ccgctctaac gaccgactct gatgtcatta tcacagaggt
    28921 tggtggaaca gtaggagata tcgagtcctt gccattccta gaggctcttc gtcagatgaa
    28981 ggcagatgtg ggtgcggata atgtcatgta tatccataca accttgcttc cttacctcaa
    29041 ggctgctggt gaaatgaaaa ccaaaccaac ccaacactct gtcaaagaat tgcgtggctt
    29101 gggaatccaa ccaaatatgt tggttattcg tacagaagag ccagctggtc aaggaattaa
    29161 aaataaactg gcccaattct gtgatgtggc accagaagcc gttatcgaat cgttggatgt
    29221 tgaacacctt taccaaattc cactgaactt gcaggcacaa gggatggacc aaattgtttg
    29281 tgatcatttg aaattagacg caccagcagc ggatatgaca gaatggtcag ccatggtgga
    29341 caaggtcatg aacctcaaga aacaagttaa gatttccctt gttggtaagt atgtggagtt
    29401 gcaagatgcc tatatctcag tggtcgaagc cttgaaacac tctggctatg tcaatgatgt
    29461 agaagtcaaa atcaattggg tcaatgccaa tgatgtgaca gcagagaatg tagcagaact
    29521 cttgtctgat gcggacggga tcatcgtacc aggtggtttt ggtcaacgtg gtacagaagg
    29581 gaaaatccaa gccatccgct atgcgcgtga aaatgatgtt ccaatgttgg gagtctgctt
    29641 gggaatgcag ttgacatgta tcgagtttgc tcgtcacgtt ttaggtcttg aaggtgccaa
    29701 ttctgcagag cttgcaccag aaacaaaata ccctatcatt gatatcatgc gtgatcagat
    29761 tgatattgag gatatgggtg gaacccttcg tttgggactt tatccgtcta agttgaaacg
    29821 tggctctaag gctgctgctg cttatcacaa tcaagaagtg gtgcaacgcc gtcaccgtca
    29881 ccgttatgag tttaataatg ccttccgtga gcagtttgag gcagcaggtt ttgtcttttc
    29941 aggagtttct ccagacaatc gtttggtaga aatcgtggaa attcctgaaa ataaattctt
    30001 tgtagcttgt cagtatcacc ctgaactgtc aagccgtcca aaccgaccag aagaactcta
    30061 cactgccttt gttactgcag cggttgagaa cagcaattag caaaatcaga acctttgaga
    30121 aaaatctcag aggttttttg catacgatga tattgcagta tatctgaggt aggggtcctc
    30181 tgtatgtacc tgctaccgtt gaaatcaata gcgactccct cttgccctgt gctagtgaat
    30241 ggatttatca gtatattgaa atgaaataaa atttgaacaa attaattcgg aaagccaaat
    30301 caatttctag caaagtttta ggaactggat t
//
LOCUS       DAVKJJ010000024        28597 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000024 DAVKJJ010000000
VERSION     DAVKJJ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28597
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.024"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            360..1523
                     /locus_tag="VW144_001094"
     CDS             360..1523
                     /locus_tag="VW144_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEU9600551.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQAFFETLFRKAKEKRSAYYQDDLTTTYRLFNQ
                     EGDGFGGLTVDLYGNYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLTMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1520..2197
                     /locus_tag="VW144_001095"
     CDS             1520..2197
                     /locus_tag="VW144_001095"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEU9600552.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQFYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKISRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2187..3041
                     /locus_tag="VW144_001096"
     CDS             2187..3041
                     /locus_tag="VW144_001096"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU9600553.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3060..4127
                     /locus_tag="VW144_001097"
     CDS             3060..4127
                     /locus_tag="VW144_001097"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU9600554.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     IAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            4137..5303
                     /gene="aroC"
                     /locus_tag="VW144_001098"
     CDS             4137..5303
                     /gene="aroC"
                     /locus_tag="VW144_001098"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU9600555.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5313..6416
                     /locus_tag="VW144_001099"
     CDS             5313..6416
                     /locus_tag="VW144_001099"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEU9600556.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEAIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITKNTDYTVVIK"
     gene            6427..6765
                     /locus_tag="VW144_001100"
     CDS             6427..6765
                     /locus_tag="VW144_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEU9600557.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6857..8140
                     /gene="aroA"
                     /locus_tag="VW144_001101"
     CDS             6857..8140
                     /gene="aroA"
                     /locus_tag="VW144_001101"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU9600558.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITVQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIAPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEICGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8133..8609
                     /locus_tag="VW144_001102"
     CDS             8133..8609
                     /locus_tag="VW144_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU9600559.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQRDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8606..9454
                     /gene="pheA"
                     /locus_tag="VW144_001103"
     CDS             8606..9454
                     /gene="pheA"
                     /locus_tag="VW144_001103"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9600560.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAIAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWLLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9451..10725
                     /gene="psr"
                     /locus_tag="VW144_001104"
     CDS             9451..10725
                     /gene="psr"
                     /locus_tag="VW144_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEU9600561.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFVYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            11004..11849
                     /locus_tag="VW144_001105"
     CDS             11004..11849
                     /locus_tag="VW144_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9600562.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVVFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKNFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            11839..12942
                     /locus_tag="VW144_001106"
     CDS             11839..12942
                     /locus_tag="VW144_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9600563.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYEIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFNVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            12944..13930
                     /locus_tag="VW144_001107"
     CDS             12944..13930
                     /locus_tag="VW144_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9600564.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAKKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGYAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            13946..15379
                     /locus_tag="VW144_001108"
     CDS             13946..15379
                     /locus_tag="VW144_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEU9600565.1"
                     /translation="MIDGKRLLFSLTIVSYTLTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGVLARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            15408..16442
                     /locus_tag="VW144_001109"
     CDS             15408..16442
                     /locus_tag="VW144_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9600566.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPEDSAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16642..17379
                     /gene="mecA"
                     /locus_tag="VW144_001110"
     CDS             16642..17379
                     /gene="mecA"
                     /locus_tag="VW144_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEU9600567.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFENLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17530..18816
                     /locus_tag="VW144_001111"
     CDS             17530..18816
                     /locus_tag="VW144_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU9600568.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18818..19687
                     /locus_tag="VW144_001112"
     CDS             18818..19687
                     /locus_tag="VW144_001112"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEU9600569.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            20027..20965
                     /gene="msrB"
                     /locus_tag="VW144_001113"
     CDS             20027..20965
                     /gene="msrB"
                     /locus_tag="VW144_001113"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9600570.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            21023..22747
                     /locus_tag="VW144_001114"
     CDS             21023..22747
                     /locus_tag="VW144_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9600571.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFLSSLLIPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVKLFKGTIRSNLTLGLNQEVSDQKLWQALEIAQAK
                     DFVSEKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFIILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            22749..24497
                     /locus_tag="VW144_001115"
     CDS             22749..24497
                     /locus_tag="VW144_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9600572.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRASLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFVKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            24799..26058
                     /locus_tag="VW144_001116"
     CDS             24799..26058
                     /locus_tag="VW144_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEU9600573.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    26134..26269
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            26308..26808
                     /locus_tag="VW144_001117"
     CDS             26308..26808
                     /locus_tag="VW144_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU9600574.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            26884..27252
                     /gene="rplL"
                     /locus_tag="VW144_001118"
     CDS             26884..27252
                     /gene="rplL"
                     /locus_tag="VW144_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU9600575.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            27439..27609
                     /locus_tag="VW144_001119"
     CDS             27439..27609
                     /locus_tag="VW144_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600576.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            27609..27860
                     /locus_tag="VW144_001120"
                     /pseudo
     CDS             27609..27860
                     /locus_tag="VW144_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            27847..28194
                     /locus_tag="VW144_001121"
                     /pseudo
     CDS             27847..28194
                     /locus_tag="VW144_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28187..28567
                     /locus_tag="VW144_001122"
     CDS             28187..28567
                     /locus_tag="VW144_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600577.1"
                     /translation="MIDLYLSKNSRRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLKLTDFIEMILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
BASE COUNT         8503 a         5308 c         6414 g         8372 t
ORIGIN      
        1 tttgtaacaa aaggcttctc tataaaatca aattttccag atatctactg aagctgattt
       61 atttcttcat ctttcttcta ctacctttgt cactaaattt gacagacaca tattgctgat
      121 acaacctctc tacctataat ctcacagtta acctgaggct agtttcatag tttgctattt
      181 gattttcgtt gataaaaaaa tgctcctcca agacggaaga gcatttttta ttgtgattct
      241 tacacattta cttcctagtt ttgcaggaag tctagtatac cattttttaa aagaagagta
      301 aaaaagcaag ggtagtttct tttttgagaa ttgtctaaaa gtttgatata atggtagtta
      361 tgaatagaat aagagtcagc aaaagggttg aaaagaagct tgctaagggg ctagttttac
      421 tagaagccag tgatcttgag aatgtcaatc ttaaggatca ggaagtagag gtgcagggtc
      481 aggaaggaaa ctttcttggg actgcctacc tttctcagca aaacaagggc ttgggctggt
      541 ttgtcagcaa agacaaggtg gccttcaatc aagctttctt tgaaacgttg tttagaaaag
      601 ccaaagaaaa gagaagcgcc tactatcaag atgatttaac aactacctat cgtctcttta
      661 atcaagaggg agatggcttt gggggtctga cagtggacct ttatggcaac tacgccgtct
      721 tttcttggta taactcttat gtttatcaga ttcgtcagac tatatcagaa gcctttagac
      781 aggttttccc tgaggtttta ggggcttatg agaaaatccg ctttaaaggt ttggactatg
      841 aatctgccca tgtttatggt caagaagcac ctgacttttt cactgtttta gaaaatggtg
      901 tcctgtatca agtctttatg aatgatggct tgatgacagg aattttccta gaccagcatg
      961 aggttcgcgg tagtttagtt gacggcttga ctatgggtaa atccttgctc aatatgtttt
     1021 cctacacagc ggctttttca gtagctgcgg ccatgggagg agctagccag acaacttctg
     1081 ttgatctagc caaacgttca cgagaattgt ctcaagcgca ttttcaggca aatgggctca
     1141 gcacagacga gcatcgtttt atagtcatgg atgtctttga gtatttcaaa tatgccaaac
     1201 gcaaagactt gacctacgat gtgattgtcc tagatccgcc tagctttgct cggaataaaa
     1261 aacaaacttt ctctgtggcc aaggattatc acaagttgat ttcccagagt cttgagattt
     1321 taaatccggg agggattatc attgccagta ccaatgctgc caatgtttcc cgtcagaaat
     1381 ttacagaaca aattgataaa ggctttgcag gaagaagtta ccagatttta aacaaatatg
     1441 gtcttccagc agattttgcc tataataaaa aagatgaaag tagtaattac ctcaaggtga
     1501 ttagtatgaa ggttagtaaa tgaaattaat cgtttcagta atgccaagaa gtttagagga
     1561 ggctcaggct ctggatgcca cgaggtacct ggatgccgac atcattgaat ggcgtgccga
     1621 ctatctgcct aaagaagcga ttttgcaggt agctccagcc atttttgaaa aattcgcagg
     1681 ccgtgagttg gttttcacgc tacgaactcg ctccgaaggt ggagaaatcg acctttctcc
     1741 agaagagtat atccatctaa tcaaggaagt tgcgcaattc tatcaaccag actatattga
     1801 ttttgagtac tatagctaca aggatgtttt tgaggaaatg ctggacttcc caaatcttgt
     1861 tttgagttac cataatttcc aagaaacacc tgagaacatg atggaaatct tgtcagagtt
     1921 gacgatccta aatccaaaac ttgttaaggt tgcggtgatg gctcacacgg agcaggatgt
     1981 cttagacttg atgaactata cacgaggctt taaaaccctc aatcctgaac aggaatatgt
     2041 gaccatttct atgggtaagg tgggcaagat ctctcgtatc actgcggatg tgactggttc
     2101 gagttggtct tttgccagtc tggatgaggt tagtgcccct gggcagattt ctctagctag
     2161 catgaaaaaa atcagggaga ttttggatga agcttgatgg ctatacacgt ttagctgcag
     2221 ttgttgccaa tcctattaag cattctattt ctcccttcat ccacaataga gcctttgagg
     2281 cgacagctac caacggtgct tatgtggctt gggagattga agcgagtgac ttggcagaaa
     2341 cagtggccaa tattcgtcgc taccagatgt ttggcatcaa tctgtccatg ccctataagg
     2401 agcaggtgat tccttatttg gataagctga gcgatgaagc gcgcttgatt ggtgcggtta
     2461 atacggttgt caatgagaat ggcaatttaa ttggatataa tacagatggc aagggatttt
     2521 ttaagtgctt gccttctttt acaatttcag gtaaaaagat gaccctgctg ggtgcaggtg
     2581 gtgcggctaa atcaatcttg gcacaggcta ttttggatgg cgtcagtcag atttcggtct
     2641 ttgttcgttc cgtttctatg gaaaaaacaa gaccttacct agacaagtta caggagcaga
     2701 caggttttaa agtggatttg tgtgctttag agtatgtttc tgaactgcaa gcaaggattg
     2761 ccgagtcgga tttgctagtt aatgccacca gtgtgggcat ggatggccaa tcctctcctg
     2821 ttcctgaaaa catagtctta ccagaaactc tcttagttgc agatatcata taccaaccct
     2881 ttgaaacacc gtttttgaaa tgggctagaa gtcagggcaa tccagcagtc aatggtctgg
     2941 gaatgttgct ctatcaagct gcagaagctt ttcaactgtg gacaggcaag gaaatgccga
     3001 cagaagagat ttggcagtct ttaacagaaa aataccaata aagaaaggga gactctccta
     3061 tgaaaatcag aatcgatatt cctcatcatc cttatgatat tcagattgaa aaaggttgta
     3121 tggcccaggc tggtcagtgg ttgcgagaac tctggcaacc gcaaaaggta gtcattgtga
     3181 cagataacca tgtagcctct ctctatgcag agaaggtcaa gctcagccta gaagatgctg
     3241 gttttcaggt agctgttttt gatttcttag aaggtgaaga aagaaagaat ttaactactg
     3301 ttcagaaagt ctatgaattt ctagtcaagc aaggtctgac tcgtagcgat ggaatcgttg
     3361 ctcttggtgg tggcgttgtt ggggacctgg ctggatttgt agcctctacc tatatgcggg
     3421 gtattcactt tgttcagatt ccgactagtt tgacagctca ggttgattct tctatcggtg
     3481 gaaagacagg cgttaacaca ccatttgcta aaaatatggt ggggaccttt gcccaaccag
     3541 atggggttct gattgatcca cttgttcttg aaaccctcgg aaaaagagag ttgattgaag
     3601 ggatgggtga agttatcaag tatggcttga ttgaggatcc agaactgtgg gctctcttga
     3661 cggggctgaa tggttctgtt gagagcattt tggaacatgc agagaccttg attgaacatt
     3721 cttgtcaggt gaagcgcaag atggtggttg aagatgagtt ggacaatggt attcgtcttt
     3781 acctcaattt tggccacact attggccatg ccatcgaagc gactgccggt tatggcaagg
     3841 tcatgcatgg agaggctgtt gccatgggaa tggtacagat ttccaagatt gctgaggaaa
     3901 aaggcctcat gccagctggc attacccaat ctatcacaga gatgtgtcag aaattcggct
     3961 tgcctgttga ctatgaaaat tgggaagttg acaagcttta tcaggctctt actcatgaca
     4021 agaaagcgcg tggtaacacc ttgaaattgg tcttggtgcc agagcttggt tcagcgacca
     4081 ttcatccagt ttctctggaa gagatgaaag actacttggt aaaataagga gagtgtatga
     4141 gatatttaac tgcaggagaa tcacacggcc cccgtctaac agctattatt gagggaattc
     4201 cagctggact tccattgaca gctgaggata tcaatgagga ccttagacgc cgtcagggtg
     4261 gctacggtcg tggtggtcgt atgaagattg agagtgacca ggttgtcttt acttcgggcg
     4321 ttcgccacgg gaagacgaca ggggcgccta ttactatgga tgtcatcaat aaggaccacc
     4381 agaaatggct ggacatcatg tctgcggagg acattgaaga ccgccttaaa agcaagcgga
     4441 aaattactca tcctcgccca ggtcatgccg atttggttgg ggggattaag taccgttttg
     4501 atgatttgcg aaattctttg gagcgttcat cagctcgtga aaccaccatg cgggtggcag
     4561 ttggtgcagt agccaaacgt ctcttggctg agctggatat ggagattgcc aaccatgtcg
     4621 tggtctttgg tggcaaggaa atcgatgttc ctgaaaatct gacagtcgct gaaattaagc
     4681 aaagagctgc ccagtctgaa gtttctattg tcaaccaaga acgagaacag gaaatcaagg
     4741 actatattga ccaaatcaaa cgtgatggtg ataccatcgg tggggttgtg gagacagtcg
     4801 tcggaggcgt tccagttggt cttggttcct atgtccaatg ggatagaaaa ttggatgcaa
     4861 gattggctca agctgttgtc tctatcaatg cctttaaagg ggtggaattt ggtcttggct
     4921 ttgaggctgg ttatcgtaaa ggcagccaag ttatggatga aattctctgg tctaaagaag
     4981 acggttatac tcgccgtacc aataatctag gtggttttga aggtggtatg actaatgggc
     5041 aacccatcgt tgttcgtggg gttatgaaac ccattcctac tctttataaa cctcttatga
     5101 gtgtggatat cgaaacccac gaaccttaca aggcaaccgt ggagagaagt gatccgactg
     5161 ctcttccagc tgcaggaatg gtcatggaag cagttgtagc aacggttctg gcgcaagaaa
     5221 tcctcgaaaa attctcatca gataatcttg aggaactaaa agaagcggta gccaaacacc
     5281 gagactatac aaagaactat taaggagttc ctatggcaaa aacaatctat atcgcaggtc
     5341 ttgggttgat tggagcctct atggcacttg gtatcaaacg cgatcatcca gattatgaaa
     5401 ttttaggtta taatcgtagt caagcttcga gagacatcgc cttgaaagaa ggcatgattg
     5461 accgtgcaac ggatgatttt gctagttttg ctcctttggc agatgtcatt atcctcagct
     5521 tgccaatcaa acaaactatt gctttcatta aggagttggc caatttggat ttgcgagaag
     5581 gcgttattat ttcagatgct ggttcgacca agtcaaccat tgtggatgcg gcggagcagt
     5641 atttggctgg caagtctgtt cgctttgtcg gggcccatcc catggctggt agtcacaaga
     5701 caggggctgc ttcggcagat gtcaatcttt ttgaaaatgc ctattatatc tttacacctt
     5761 caagcctgac aagtcaggac acgcttaagg aaatgaagga tctgctttca ggtcttcatg
     5821 ctcgttttat cgagattgat gccaaggagc atgatcgtgt cacttctcag attagccatt
     5881 ttcctcatat tttggcttct agtctcatgg agcagactgc ggtctatgct caagagcatg
     5941 agatggcaag gcgctttgcg gcaggtggtt ttcgagatat gacccgaatt gcggaaagcg
     6001 agccaggaat gtggacctcc attctcttgt ccaatagcga gaccattctg gatagaattg
     6061 aggatttcaa ggaacgtttg gaagcgattg gtcaggccat tagtaaggga gatgaagagc
     6121 aaatttggaa cttttttaac caagcgcgtg aacaacgcca gacaatggaa atccataagc
     6181 gtggtggtgt ggatagctct tacgacctct atgttgacgt tcctgatgaa gaagatgtca
     6241 tcctgcggat tttggaactg ctacgtggga cttccttggt taacatccac atcaacgagg
     6301 aaaatcgtga ggatattcac ggaatcctac aaatttcatt taaaaatgct caagacttgg
     6361 aaagagccga gcatctcata acaaaaaata ccgactatac agtcgtcatt aaataaggag
     6421 aagaacatgt caaatattta cgatagtgca aacgaactca gtcgcggtct acgcggatta
     6481 ccagaataca aggctgttaa agcagctaaa gatgcgattg cagcagatgc tgaggcaagc
     6541 aaaatcttta cagaatatct tgcctttcaa gaggaaattc aaaaactagc acagacaggt
     6601 caaatgccag atgcgtcctt ccaagctaag atggaaggct ttggtaaaca gattcaagga
     6661 aatagcctct tgtcagaatt ctttaccaag caacaacaat tggccattta cctttctgac
     6721 attgaaaaaa ttgttttcga accagtttca gaattgctga aataagaaaa gaactatcct
     6781 aaagtcagga atttttaagg aatttctggc tttttatgat aaaataggta aaagtattgc
     6841 aagtatgagg tccagtatga aactaaaaac aaacattcgc catttacatg gtagtatccg
     6901 cgtcccaggt gacaagtcta tcagccaccg ttccattatc tttggaagtt tggctgaggg
     6961 tgagaccaag gtttatgata ttctgcgagg tgaagacgtt ctttcgacca tgcaggtttt
     7021 tcgtgacctt ggtgttgaaa ttgaggataa agatggggtt attaccgttc aaggtgtagg
     7081 catggctggc ttaaaagcgc cgcaaaatgc ccttaatatg ggaaattctg gcacctcgat
     7141 tcgcctgatt tcaggtgtcc ttgctggtgc agatttcgaa gtagagatgt ttggagatga
     7201 tagtctttcc aaacgtccta tggaccgtgt gacccttcca ctgaaaaaaa tgggcgtcag
     7261 catctcaggg caaactgaac gagacttgcc tccccttcgc ttaaaaggga cgaaaaacct
     7321 aagacctatt cattatgagt tgccaattgc ctctgcccaa gtcaagtcag ccttgatgtt
     7381 tgcagcctta caagctaagg gggagtcagt tattatcgaa aaagagtaca cccgtaatca
     7441 tactgaagat atgttgcaac aatttggtgg tcatttaagt gtggatggta agaaaatcac
     7501 agtccaaggg ccacaaaaat tgacaggaca gaaggtggtc gtaccaggag atatttccag
     7561 tgcagccttt tggttagtcg caggtttgat tgctccaaat tctcgtctag tgctgcagaa
     7621 tgtggggata aacgaaactc gcaccggtat tattgatgtc attcgtgcca tgggtggaaa
     7681 attggaaata actgaaatcg atccagtcgc taaatctgca accttgattg ttgagtcttc
     7741 tgacttgaaa ggaacagaga tttgtggcgc tttgattcca cgtttgattg atgaattgcc
     7801 tattattgcc ctacttgcga cccaagccca aggtgtaaca gttatcaagg atgctgagga
     7861 gctcaaggtc aaggaaacag accgtattca ggttgtggca gacgccttaa atagtatggg
     7921 agcagatatt actcctacgg cagatgggat gattatcaaa ggaaaatcag ctcttcacgg
     7981 tgctagagtc aatacgtttg gtgaccaccg tatcggcatg atgacagcta tcgcagccct
     8041 attggttgca gatggagagg tggagcttga ccgtgcagaa gccatcaata ccagctatcc
     8101 tagtttcttt gatgatttgg agagcttgat tcatggctaa ggtgttatta gggtttatgg
     8161 gggctggaaa atcgactatt gcaagaggct tggaccctaa ttaccttgat atggatgctc
     8221 tgattgagaa gcgcctaggt atgtccattg cgaatttttt cgctgaaaag ggagaagcag
     8281 cctttcgtca ggtagaatca gaagtcctag ctgatttatt acaaagagac caagtcgtgt
     8341 caactggagg aggagtggtt atttctcaga gaaatcgtga cttactcaag actaatacag
     8401 ataacatcta cctgaaagca gattttgaaa ccctctacct acgtatcgca gctgataagg
     8461 acaatcagcg accgcttttt ctaaataata gcaaggaaga actagtagct atttttcaag
     8521 aaagacaggc ttggtatgag gaagtggcta gtcgggtttt ggatgtgacc aagctaagcc
     8581 cagaggaaat tatagaggaa ctaagatgaa aattgcttat ctaggtccca aaggatcatt
     8641 ttcacaccac gttgtgcaga cagcttttcc tcatgaggaa ttgcaggctt ttgccaacat
     8701 tacagatgtc atcaaggctt atgagcaagg attagtggac tattctgtgg tgccagttga
     8761 aaattctatt gagggtagtg ttcatgaaac cttggactat ctttttcatc aggctcacat
     8821 ccaagcagta gcagaaatcg ttcagcctat tcatcagcag ttgatggtgg ttccaggcca
     8881 tactaagatt gaaaagattt tttcacatcc acaggccttg gctcaaggaa agaaattcat
     8941 cgatgaacaa tatccagagg ctcaaatcga ggtaacagct agtacagctt atgcggcccg
     9001 ttttatttcc gaacatccag atcagccctt tgcagcaatt gcacctagaa gttctgctga
     9061 agaatatgga ttggaactga ttgccgagga tattcaggaa atggaagcca atttcacacg
     9121 tttctggctt ctaggagctg aaaagcctag tattcccttg caagcacaaa ctgaaaagat
     9181 gagtttggcc ttgacattac ctgacaacct tccaggtgca ctttataagg ccctgtcgac
     9241 ctttgcttgg cgaggaattg acttgacaaa aattgaaagt cgtccactca agacagcact
     9301 gggtgaatac tttttcatta tcgatgtgga ttataccgat aaggacttgg tccactttgc
     9361 ccaaaaagaa ttagaagcga ttggaatcca gtataaaatt ctgggtgcct atcctattta
     9421 tccaatatca gaccatggaa aggagagaag atgagtaaag aaaatccctt aagtcatcat
     9481 gagcagttgc gttatgatta tttgctaaaa aatattcact atctcaatga gagagaaaaa
     9541 aatgagtttg tctatttgca agaaaagcta actcttgcta ggggaaatag tagctctagc
     9601 ttggaacaag aaagagaaga gcaggttgac ttaccaagct atgcgaaccg gagtcgctca
     9661 caatccaaat cacaagcact ctctttccct ccaaaaaaga aaagacggaa gctccgtctt
     9721 aaacgaattt ttatggtgat tttttcactt ttggtctgtg tggctttggc catggtattc
     9781 atgtttttgc gtggttacca agatgctagc gcaaagaaaa ctgctgatgc ccgggcagct
     9841 caagtagaag tctttaatgg tcaggacact agagatggag ttaatatttt aatcatgggt
     9901 actgatggtc gaatcggcca gaacagtgtt gagacacgaa ctgactctat tatggtatta
     9961 aatgtcgggg gctcagataa gaaaatgaag ctagtcagtt tcatgcgtga taatttggtc
    10021 tatatagatg gttatagtca agtgattaac ggtagaaaac agacagataa caagttaaac
    10081 gtagcctacg agttaggaga acaagaaggg caaaaagggg cagaaatggt tcgccaagtc
    10141 ttgaaagata attttgactt ggacattaag tactacgcct tggttgattt ccaggccttt
    10201 gccacagcga ttgacacgct tttccctgat ggggtgacga ttgatgctca attttcaaca
    10261 ttgaatgggc gtcccctaac agaagctaca gtcggagatg atttatacgc taccgagact
    10321 gagtctccaa cccaaaccat taaagtcgga aaacagcaga tgaatggctc gaccctgctc
    10381 aattatgctc gtttccgtga tgatgatgag gcggattacg gccgtacaaa aagacagcaa
    10441 caagttttaa cagcaatttt agagcaaatt aaagatccca ctaaactttt cactggttct
    10501 gaagctcttg gaaaggtttt tgctatgacc tcaaccaacg taccctatac tttcctccta
    10561 acaaacggtt tatctgtttt ggatggagca aaaaatggta ttgaaaaatt gacgattcca
    10621 gaactgggtg actgggtaga tgcctatgat gtttatggag gcttgggctt gctggttgat
    10681 caaaacaaat atcaaaccaa gctcgctcaa atgggcttga gataagataa tactcaatga
    10741 aaatcaaagt gcaaattagg aagctagccg caggctgcta aaaacacagt tttgaggttg
    10801 tagataagac tgacgaagtc agttacctat acctacggca aggtgaagct gacgttgttt
    10861 gaagagattt tcgaagagta taagacagaa aaatcaaagt ttgatagcta tttggatagc
    10921 agtcaggctt tgatttttta cagtctgcaa tagattttca ccccttgttt gtggtataat
    10981 gaaacgatac gatagaaaga ataatgaaca atatgactga tttaaaagca attcaggctc
    11041 gtagtctgga gatggctgaa tattttgtgg tcttttgtaa agaacatgat ttgctctgtt
    11101 atctctgtgg cggaggcgct attggtgccc ttcgaaacaa gggatttatt ccttgggacg
    11161 acgacctaga cttttttatg cctcgtaaag attatgagaa attagcagaa ttatggcctc
    11221 gttatgcaga tgaacgttat ttcttgtcaa agagtcacaa gaattttgtt gatcgtaatc
    11281 tttttattac cattcgtgac aaggaaacca cctgtatcaa gccttatcag caggatttgg
    11341 atttgccaca tggtctggcc ttggatgttt tgcctttgga ttattatccg aaaaatccag
    11401 ctgagcggaa aaaacaggtt cgttgggcct tgatttattc actcttttgt gcgcaaacta
    11461 ttccagaaaa gcatggtgct ctcatgaaat ggggaagtcg cattttactg ggtttgactc
    11521 caaaatctct ccgttatcgc atctggaaaa aagctgagaa agaaatgact aagtatgatt
    11581 tggctgattg tgatggcatt acagaattat gctcaggtcc tggctacatg agaaacaagt
    11641 acccaatcac atcttttgaa gacaatcttt tcttgccatt tgaaggaaca gagatgccta
    11701 ttccaatcgg ctatgatgtc tatctcagaa ctgcttttgg ggattatatg acgcctccac
    11761 cagcagacaa gcaggtaccg catcatgatg ctgtcatcgc tgatatggat aagtcttata
    11821 cagaatacaa gggagaatat ggtggctaag aaaaaaatct tattttttat gtggtctttt
    11881 tctcttggag gtggtgcaga gaagattcta tcaaccattg tttcaaatct ggatccagaa
    11941 aagtatgata ttgatattct tgaaatggag cactttgaca agggatatga atctgttcca
    12001 aagcatgtac gcattttaaa atcccttcaa gattatcgcc aaaccagatg gttacgagct
    12061 tttttgtgga gaatgagaat ttattttcca agactgactc gtcgtttgct tgtaaaagat
    12121 gattatgatg ttgaagtttc ttttaccatt atgaatccac cactgttgtt ctctaaaaga
    12181 agagaagtca agaagatatc ttggattcat ggaagtattg aagaacttct taaggatagc
    12241 tctaaaagag aatcacatag aagccagttg gatgctgcga atacaattgt agggatttca
    12301 aaaaagacca gcaattctat caaggaagtt tatccagatt atgcttctaa attacagaca
    12361 atctacaatg gatatgattt tcagactatt ctagaaaaat ctcaagagaa gatcgatatc
    12421 gagattgctc ctcaaagtat ctgtactatc ggacggattg aggaaaataa gggttctgac
    12481 cgtgtagtgg aagtgatacg attattacac caagagggaa aaaactatca tctctatttt
    12541 atcggggctg gtgatatgga agaggaactg aaaaaacgag tcaaagagta tgagattgag
    12601 gactatgtac atttccttgg ttatcaaaaa aatccttatc agtatttatc tcagacgaaa
    12661 gttctcttgt ctatgtctaa acaagaaggc tttcctggag tgtatgtgga ggccttgagt
    12721 ctgggactcc cttttatctc tacggacgtt ggaggggctg aggaattatc ccaagaagga
    12781 cgatttggac aaatcattga gagcaatcaa gaggcagctc aggcgattac taattacatg
    12841 acttctgcct caaactttaa tgtcgatgag gctagccaat tcattcaaca atttacaatt
    12901 acaaaacaaa tcgaacaagt agaaaaacta ttagaggagt agcatggaaa ctgcattaat
    12961 tagtgtgatt gtgccagtct ataatgtggc gcagtaccta gaaaaatcga tagcttccat
    13021 tcagaagcag acctatcaaa atctggaaat tattcttgtt gatgatggtg caacagatga
    13081 aagtggtcgc ttgtgtgatt caatcgctga acaagatgac agggtgtcag tgcttcataa
    13141 aaagaacgaa ggattgtcgc aagcacgaaa tgatgggatg aagcaggctc acggggatta
    13201 tctgattttt attgactcag atgattatat ccatccagaa atgattcaga gcttatatga
    13261 gcaattagtt caagaagatg cggatgtttc aagctgtggt gtcatgaatg tctatgctaa
    13321 tgatgaaagc ccacagtcag ccaatcagga tgactatttt gtctgtgatt ctcaaacatt
    13381 tctaaaggaa tacctcatag gtgaaaaaat acctgggacg atttgcaata agctaatcaa
    13441 gagacaaatt gcaactgccc tatcctttcc taaggggttg atttacgaag atgcctatta
    13501 ccattttgat ttaatcaagt tggccaagaa gtatgtggtt aatactaaac cctattatta
    13561 ctatttccat agaggggata gtattacgac caaaccctat gcaaagaagg atttagccta
    13621 tattgatatc taccaaaagt tttataatga agttgtgaaa aactatcctg acttgaaaga
    13681 ggtcgctttt ttcagattgg cctatgctca cttctttatt ctggataaga tgttgctaga
    13741 tgatcagtat aaacagtttg aagcctattc tcagattcat cgttttttaa aaggctatgc
    13801 ttttgctatt tctaggaatc caattttccg taaggggaga agaattagtg ctttggccct
    13861 attcataaat atttccttat accgattctt attactgaaa aatattgaaa aatctaaaaa
    13921 attacattag aacgggggtg accaagtgat tgatgggaaa cgattattat ttagtttgac
    13981 catagtcagt tataccttga cgctagtaag tggaattgtg tatctgttta ataataataa
    14041 tgttagctta ctttctactt tattgttctt gttggttagt agcttaattg cttgttggaa
    14101 tgatatcaag tattacttaa tccattttat tttctattta accatttttg tatttctggt
    14161 atcaagaccg accattgatt attttaggga tggtgctttg gatacctatc atccaatagc
    14221 ctatcgtttt gcctttatag ttgtcatggt ttcgattctg ggcttgacca caggaggcgt
    14281 cctggctcgt tacttcatag ctaggaagaa aataaaagta gcaaatatag gaaattctct
    14341 aaaagaggtt tatatcaagc ggttacgctt tgtatcacta ggagtttttc ttctaactta
    14401 tcctttctat ttcattcggt tatttgaacg gctcttgtat cgtttgcaga cttcctacta
    14461 tgcctactat gcaaattttg aaagtaaact gccttatttt acctacattt tgtctacctt
    14521 tacggtctat gcaatgtgta tgtatctggc aaccaagcca aagaaattgc aggccacagc
    14581 agtgcttgtc tcctttattg cagctaatac tattcatttg gcaattggga cacgaaatcc
    14641 ctttatttta agtattttat ttgcttttgt ttattacttt atgcgggagc aaactgaaaa
    14701 aggaaaatgg attgggttta aagaaaagtt agcgattttt gtaggttctc ctattctcat
    14761 gttagcgatg ggagtactca attatgtacg ggataatgtc caagtttccc atacaggttt
    14821 ctgggatatc ttacttgact ttatctataa acaagggact agttttggtg ttctggctcg
    14881 aggttttcta tttaacagta gcctccctta ccgagatttc cgtaatttta cttttggtcc
    14941 tgttcttgat tattttgcaa gggggagttt gggagccatt ttcggaggaa aagcctttga
    15001 acatacaacc aatagtgtgg aactagctat tgatagtaat agttatgccc acaatctatc
    15061 ctatcttgtc ttgaacaagg aatacttgaa agggcatggt atcggaagta gttatatcat
    15121 ggagttgtat accgactatg gtatgattgg agtctttctg cttagtttct tactcggcgt
    15181 attatttata gccatgctgc aagtagccta tcgctcaagg acaatcctat ttgctttatc
    15241 cctactcatc ttgaataatc tattctttat gccaagaagc agcttttcag aaagtttctt
    15301 caatttattt acaatgcaat tctggggaat tgttcttgtg attatatttg tagcaaaaat
    15361 gcttacaaag gaaaactagt atctactaaa caaaggagaa aaaaatcatg tttgaacatt
    15421 attcagtagc tgatttgttt gcaaatcttt ataaaaagcg aaaagcaaat attttagctc
    15481 tcatcgcttt atttgctctc attgctgtac catttacaat taaagcagtt aggaataaaa
    15541 acactgtcaa agatacaaca agttattcaa cttatcttat ctataaaatc actcctccag
    15601 aagattcggc caaaacgatt ttgaatcatc aaattggtgg ttatagtgat ttttatggga
    15661 aattgattga tggtaatttg aatggagctt atcttttcaa tgatgtagaa cccagtgagt
    15721 tgaaaaaaat tgccagtgaa ttagacacga cagaaacaac cttgaaaaat tcaacgaatg
    15781 actattggtg gaaaaaattg accgtctact atatgattga cgatgcaggg gttggtgtga
    15841 aaattttgac atcaagtaaa gatgccaatg acttgttaga gaaaaaaatt gatgggttga
    15901 ttgagaaatt taaacatgcc tatgcaaatg tgaaaattga aaaactggaa accatcaact
    15961 ctaaagaatt gaacgcaaat ggtgaaacag cgcttggctt aaatgtgaaa aatctgattc
    16021 ttcgtttagt tgttattgga gtggtttgtg tgattttggt tgtgatggga aatgtgttag
    16081 tttatctctt taatccaaca atcaatagag taggtgattt ttctcagtat caaattgatt
    16141 ttgtaacaga gattacaaca attgctaacc tagcagatgt tttgtcatac aaaaatactg
    16201 gacaggaatt gaccatcgtt agctcaaata aagctatcct agataaatta aaacagagtc
    16261 aagaagcttt aaaaggaatg cattttgtag atttacagga tgtatcatct cttttggaaa
    16321 gagatacagt ccttcttgtt gaagagtacg gagtgactcg ttataagaaa tttgagcaaa
    16381 gtcttcaaat tctcagaaac ttaaatcgtt ctatcctcgg tgtagcaacc tttaaattat
    16441 aagtattctg atgtataagg tcttcaaagt cggctaagag ccgattttga aggctttttc
    16501 tatctgtctg cagaaatttt cttttggagt ctcctataga aagacccttg ggaaaatcta
    16561 tccgcataga ggagccttct tttgataaaa tcgtaaaaat ctagtataat agatagaact
    16621 gaaagtatga ggttactagc aatgaaaatg aaacaaatta gtgatacaac tttaaaaatc
    16681 acgatgtctt tagaggattt gatggatcgt ggaatggaga ttgctgactt tctcgttcct
    16741 caagaaaaaa cagaagagtt cttttatgct atcttggatg agctagagat gcctgatagc
    16801 tttctggata caggcatgtt gagcttccgt gtgactccaa aacctgataa ggtagatgtc
    16861 tttgtgacca agtcaaagat tgaccaaaat ctagattttg aaaacttatc ggatttgcca
    16921 gatatggaag aattggctca aatgtctcca gatgaattta tcaaaaccct ggaaaaaagc
    16981 atcgcagaca aaaccaagga tgatatcgaa gccattcaat ctcttgagca agttgaagcc
    17041 aaggaagaag agcaagagca ggctgaacaa gaagctgaga gtaagaaaga accttacatc
    17101 tactacatcc tttcttttgc taagttggct gacttggtag cttttgccaa gacagtgact
    17161 tttgaaatgg aaacttctga actctacaaa atgaacgagc gctattattt gaccatttta
    17221 gtggatattg aaaatcatcc aagcccatat ccagcttggc tgttggcccg tatgcgcgag
    17281 tttgcagacg atagtgatat cagtcgctca gtcttacaag agtatggtca agtcttgatg
    17341 agtcacgatg cagtgctcaa tctgcaaaaa atcggctaat cttttctgga aagctatttt
    17401 cagattttaa gaagagcgaa tcgtctgatt cgttttttct ttttatactg aaatagtgat
    17461 ttactataat aggaattttc acaaaattct gttataatgg ctatattaga aaatttcgag
    17521 gagataaaga tgacagttaa aattgcttta ctaggatttg gtaccgttgc aagtggtgtg
    17581 cctttcctcc taaaggaaaa tggaggaaaa atcaatcaat cagcacattc agatatcaaa
    17641 gttgctaagg tattggtcaa ggatgaagat gaaaaaaatc gcttgcttgc agcagggaat
    17701 gactttaact ttgtaaccaa tgtggatgat attttatcag accaggatat tactatcgta
    17761 gtggaattga tggggcgtat tgagcctgct aaaaccttta tcactcgtgc cttggaagct
    17821 ggaaaacacg ttgttactgc taacaaggac cttttagctg tccatggcgc agaattgcta
    17881 gaaatcgctc aagctaacaa ggtagcactt tactacgaag cagcagttgc tggtgggatt
    17941 ccaattcttc gtactttagc aaattccttg gcttctgata aaattacgcg cgtgcttgga
    18001 gtagtcaacg gaacttccaa cttcatggtg accaagatgg tggaagaagg ctggtcttac
    18061 gatgatgctc ttgcggaagc acaacgtcta ggatttgcag aaagcgatcc gacgaatgac
    18121 gtagatggga ttgatgcagc ctacaagatg gttattttga gccaatttgc ctttggcatg
    18181 aagattgcct ttgatgatgt agcccacaag ggaatccgca atatcacacc agaagacgta
    18241 gctgtagctc aagagcttgg ttacgtagtg aaattggttg gttctattga ggaaacttct
    18301 tcaggtattg ctgcagaagt gactccaacc ttcctaccta aagcgcaccc acttgctagt
    18361 gtgaatggcg taatgaacgc tgtctttgta gaatctatcg gtattggtga gtctatgtac
    18421 tacggaccag gtgcgggtca aaaaccaact gcaacaagtg ttgtagctga tattgtccgt
    18481 atcgttcgtc gtttgaatga tggtactatt ggcaaagact tcaacgaata tagccgtgac
    18541 ttggtcttgg caaatcctga agatgtcaaa gcaaactact atttctcaat cttggctcta
    18601 gactcaaaag gtcaggtctt gaagttggct gaaatcttca atgctcaaga tatttccttt
    18661 aagcaaatcc ttcaagatgg caaagagggt gacaaggcgc gtgtcgttat catcacacac
    18721 aagattaata aagcccagct tgaaaatgtc tcagctgaat tgaagaaggt ttcagaattc
    18781 gacctcttga ataccttcaa ggtgctagga gaataagatg aagattattg tacctgcaac
    18841 cagtgccaat atcgggccag gttttgactc ggtcggtgta gctgtaacca agtatcttca
    18901 aattgaggtc tgcgaagaac gagatgagtg gctgattgaa caccagattg gcaaatggat
    18961 tccacatgat gagcgtaatc tcttgctcaa aatcgctttg caaattgtac cagacttgca
    19021 accaagacgc ttgaaaatga ccagtgatgt ccctttggcg cgcggtttgg gttcttccag
    19081 ctcggttatt gttgctggga ttgaactagc caaccaactg ggtcaactca acttatcaga
    19141 ccatgaaaaa ttgcagttag cgaccaagat tgaagggcat cctgacaatg tggctccagc
    19201 catttatggt aatctcgtta ttgcaagttc tgttgaaggg caagtctctg ctatcgtagc
    19261 agactttcca gagtgtgatt ttctagctta cattccaaac tatgaattac gtactcgcga
    19321 cagccgtagt gtcttgccta aaaaattgtc ttataaggaa gctgttgctg caagttctat
    19381 cgccaatgta gcggttgctg ccttgttggc aggagacatg gtgaccgctg ggcaagcaat
    19441 cgagggagac ctcttccatg agcgctatcg tcaggacttg gtaagagaat ttgcgatgat
    19501 taagcaagtg accaaagaaa atggggccta tgcaacctac ctttctggtg ctgggccgac
    19561 agttatggtt ctggcttctc atgacaagat gccaacaatt aaggcagaat tggaaaagca
    19621 acctttcaaa ggaaaactgc atgacttgag agttgatacc caaggtgtcc gtgtagaagc
    19681 aaaataaaga atagaagata ggatggggaa actcttgacc agagggtttc atatcctttt
    19741 tgtgaaaaga agtttatact cagtgaaaat caaagagcaa actaggaagc tagccgcagg
    19801 ttgctcaaag cactgctttg aggttgtaga taagactgac gacgtcagct caagacactg
    19861 ttttgaggtt gtggatagaa ctgacgacgt cagctcaaga cactgttttg aggttgcaga
    19921 tagaactgac gaagtcagta acatatatac ggtaaggtga cgctgacgtg gtttgaagag
    19981 attttcgaag agtattagtt aaaaacgtga taaaggagaa ataaaaatgg cagaaattta
    20041 tctagcaggt ggttgttttt ggggcctaga ggaatatttt tcacgcattt ctggagtgct
    20101 agaaaccagt gttggctacg ctaatggtca agtcgaaacg accaattacc agttgatcaa
    20161 ggaaacagac catgcagaaa cggtccaagt gatttacgat gagaaggaag tgtcactcag
    20221 agagatttta ctttattatt tccgagttat cgatcctcta tctatcaatc aacaagggaa
    20281 tgaccgtggt cgccaatatc gaactgggat ttattatcag gatgaagcag atttgccagc
    20341 tatctacaca gtggtacagg agcaggaacg catgctgggt cgaaagattg cagtagaagt
    20401 ggagcaatta cgccactaca ttctggctga agactaccac caagactatc tcaggaagaa
    20461 tccttcaggt tactgtcata tcgatgtgac cgatgctgat aagccattga ttgatgcagc
    20521 aaactatgaa aagcctagtc aagaggtgtt gaaggccagt ctatctgaag agtcttatcg
    20581 tgtcacacaa gaagctgcta cagaggctcc atttaccaat gcctatgacc aaacctttga
    20641 agaggggatt tatgtagata ttacgacagg tgagccactc ttttttgcca aggataagtt
    20701 tgcttcaggt tgtggttggc caagttttag ccgtccgatt tccaaagagt tgattcatta
    20761 ttacaaggat ctgagccatg gaatggagcg aattgaagtt cgttctcgtt caggcagtgc
    20821 tcacttgggt catgttttca cagatggacc gcgggagtta ggcggcctcc gttactgtat
    20881 caattctgct tctttacgct ttgtggccaa ggatgagatg gaaaaagcag gatatggcta
    20941 tctattgcct tacttaaaca aataaaacag agagtggggc ttcccacttt cttcatttct
    21001 agaatatgaa tagaagggat ttatgaaaca cctattatct tacttcaaac cctacatcaa
    21061 ggaatcaatt ttagccccct tgttcaagct gttagaagct gtttttgagc tcttggttcc
    21121 catggtgatt gctgggattg ttgaccaatc tttacctcag ggagatcaag gtcatctctg
    21181 gatgcagatt ggcctgctcc ttatctttgc agtaattggc gttttagtgg ccttgatagc
    21241 tcaattttac tcagcaaagg cagcagtagg ttttgctaag gaattgacaa acgatcttta
    21301 tcgtcatatt ctttccttgc ccaaggacag cagagaccgt ctgacaactt ctagtttggt
    21361 tactcgcttg acttcggata cctaccagat tcagactggt atcaatcaat tcctgcgtct
    21421 ctttttacga gcgcccatta tcgtttttgg tgccattttt atggcttatc gaatctcagc
    21481 tgagttgact ttctggttct tagtcatggt tgccattttg accattgtca ttgtagggtt
    21541 atctcgattg gtcaatcctc tctacagtag tctcagaaag aaaacggacc aactggttca
    21601 ggaaacgcgc cagcaattgc aagggatgcg ggttattcgt gcttttggtc aagaaaaacg
    21661 agagttacag atttttcaaa cccttaacca agtttatgct agattacaag aaaagacagg
    21721 tttcttgtct agtttattaa tacctctgac ctatctgatt gtcaatggaa ctcttctcgt
    21781 tattatctgg caaggctata tttcaattca aggaggagta ctcagtcaag gtgctctcat
    21841 tgctcttatc aattacctct tacagatttt ggtggaattg gtcaagctag ccatgctgat
    21901 caattccctc aaccagtcct atatctcagc caagagaatc gaggaggtct ttgcggaatc
    21961 tcccgaaaac atccattcag aattagaaca aaagcaagtt accagtggtc gggttttaca
    22021 agtccaagaa ttgactttta cctatcctga tgcggcccag ccttctctga gagacatttc
    22081 ctttgatatg actcaaggac aaatccttgg tatcattggg gggactggtt ctggtaaatc
    22141 aagcttggtg caactcttac ttggacttta tccagtagac aaggggaaca ttgaccttta
    22201 tcaaaatgga cgtagtcctc ttaatttgga gcagtggcgg tcttggattg cctatgtacc
    22261 tcaaaaggtc aaactcttta agggaactat tcgttccaac ttgactttag gtttaaatca
    22321 agaagtatct gaccagaaac tctggcaggc cttggagatt gcgcaagcta aggattttgt
    22381 cagtgaaaag gaaggacttt tggatgccct agttgaggca ggggggcgaa atttctcagg
    22441 tggacaaaaa caaaggttgt ctatcgcccg agcagtcttg cgccaagctc cgtttatcat
    22501 cctagatgat gcaacctcgg cactggatac cattacagag tccaagctct tgaaagctat
    22561 tagagaaaat tttccaaaca cgagcttaat tttgatctct caacgaacct cgactttaca
    22621 aatggcggac cagattctcc tcttggaaaa aggtgagttg ctagctgttg gcaagcacga
    22681 tgacttgatg aaatccagcc aagtctatcg tgaaatcaat gcatcccaac atggaaagga
    22741 ggactagaat gaaacgacaa actgtaaacc agacgctcaa acgtttagcc gtagatttag
    22801 caaaccatcc cttcctcctt ttcctagcct ttctaggaac tattgcccaa gttggcttat
    22861 caatttacct acctattctg attgggcagg tcattgacca agtcctagtg gctggttcat
    22921 caccagtttt ttggcagatt tttctccaga tgctcttggt ggtaatagga aatactctgg
    22981 tacaatgggc caatcctctc ctctataatc gtctaatctt ctcttatacc agagatttac
    23041 gggagcgaat catccataaa ctccatcggt taccgattgc ctttgtagat aggcaaggta
    23101 gtggagagat ggttagtcgt gtaaccacgg acatcgaaca gttggcagct ggcttgacca
    23161 tgatttttaa ccaatttttc attggtgttt tgatgatttt ggttagtatt ctagccatgc
    23221 tccaaattca tctcctcatg actctcttag tcttgctgtt gacgccactg tccatggtga
    23281 tttcacgctt tattgccaag agatcctatc atctcttcca gaagcaaaca gagacgaggg
    23341 gaattcagac tcagttgatt gaagaatcgc ttagccagca gactataatc cagtccttca
    23401 atgctcaaac agaatttatc caaagattgc gtgaggctca tgacaactac tcaggctatt
    23461 ctcagtcagc catcttttat tcttcaacgg tcaatccttc gactcgcttt gtaaatgcac
    23521 tcatttatgc ccttttagct ggagtaggag cttatcgtat catgatgggt tcaaccttga
    23581 ccgtcggtcg tttagtgact tttttgaact atgttcagca atacaccaag ccctttaatg
    23641 atatttcttc agtgctagct gagttgcaaa gtgctctggc ttgcgtagag cgtatctatg
    23701 gagtcttaga tagccctgaa gtggctgaaa caggtaagga agtcttgacc agtgaccaag
    23761 ttaagggagc tatttccttt aaacatgtct cttttggcta ccatcctgaa aaaattttga
    23821 ttaaggactt gtctatcgat attccagctg gtagtaaggt agccatcgtt ggtccgacag
    23881 gtgctggaaa atcaactctt atcaatctcc ttatgcgttt ttatcccatt agctcgggag
    23941 atatcttgct ggatgggcaa tccatttatg attatacacg agcatcattg agacagcagt
    24001 ttggcatggt gcttcaagaa acctggctca cacaagggac cattcatgat aatattgcct
    24061 ttggcaatcc tgaagccagt cgagagcaag taattgctgc tgccaaagca gctaatgcag
    24121 actttttcat ccaacagttg ccacagggat acgataccaa gttggaaaat gctggagaat
    24181 ctctctctgt cggccaagct cagctcttga ccatagcccg agtctttctg gctattccaa
    24241 agattcttat cttagacgag gcaacttctt ccattgatac acggacagaa gtgctggtac
    24301 aggatgcctt tgtaaaactc atgaagggcc gcacaagttt catcattgct caccgtttgt
    24361 caaccattca ggatgcggat ttaattcttg tcttagtaga tggtgatatt gttgaatatg
    24421 gtaaccatca agaactcatg gatagaaagg gtaagtatta ccaaatgcaa aaagctgcgg
    24481 cttttagttc tgaataagcc attctctttt gaaagtttat ggacgaaaaa agttgccttc
    24541 gagtgacttt tttgttacaa tagctagaaa aattgttcac tgtaatactc aatgaaaatc
    24601 aaagagcaaa ctaggaagct agccgtaggt tgctcaaaac actgttttga ggttgcagat
    24661 agaactgacg aagtcagctc aaaacactgt tttgaggtta cagatagaac tgacgaagtc
    24721 agtaaccaca cctacggcaa agtgaatctg aagtggtttg aagagagtac aacttgtctt
    24781 ttagaaaagg agcctataat gaaagtcttt cagcatgtaa atatcgtgac ttgtgatcaa
    24841 gatttccatg tttatcttga tggaatctta gcagtcaagg attctcaaat cgtctatgtc
    24901 ggtcaagata agccagcgtt tttagagcaa gctgagcaga ttatagacta tcagggagct
    24961 tggattatgc ctggtttggt caattgtcac acccattctg caatgacagg tctgagaggg
    25021 atccgagatg acagcaatct ccatgaatgg ctcaatgact atatttggcc agcagaatct
    25081 gagtttactc ccgacatgac taccaatgcg gtcaaagaag ccctgacaga gatgctccag
    25141 tcaggaacaa caacctttaa cgatatgtat aatcccaatg gtgtggatat ccagcaaatt
    25201 tatcaggtgg tgaaaacttc caagatgcgt tgttattttt ctccgactct cttttcttca
    25261 gagacagaaa caactgctga gactataagt agaactcgat ccatcataga cgaaatctta
    25321 aaatataaaa atccaaattt caaggttatg gtagcacctc attctccgta tagctgcagt
    25381 agagacttgc tggaagcgag tttggaaatg gcaaaagagc taaatattcc gctccatgtc
    25441 catgtggcgg agaccaagga ggagtcagga attatcctca aacgatacgg caaacgcccc
    25501 cttgcttttc ttgaagaact gggttattta gatcatccgt ccgtatttgc tcacggggtc
    25561 gaattaaacg agagagaaat tgaacgcttg gcatcttctc aagtggctat cgcccacaat
    25621 cctatcagta acctcaaact ggcatcagga attgctccaa ttatccagct ccaaaaagcg
    25681 ggagtagcag tcggaattgc gactgactcg gttgcttcca ataacaatct agatatgttt
    25741 gaggaaggaa ggactgcagc tcttcttcag aagatgaaaa gtggggatgc cagccagttt
    25801 ccaatcgaaa cagctctcaa ggtactgaca atcgaagggg ctaaggtcct tggaatggaa
    25861 aatcagatag gaagtctgga agtcggcaag caagcagatt ttctggtcat tcaaccacaa
    25921 gggaaaattc atctccaacc tcaggaaaat atgctgtctc acctggttta tgcagttaaa
    25981 tctagtgatg tagatgatgt ttatatcgcc ggagaacagg ttgttaagca aggtcaagtc
    26041 ctgacagtag aactttaaaa gaaaaattac gaaaaatttt aaaaaaagtt tgcaaaaatc
    26101 ttgcattctt tttttgacta tgctatactt atatacggtt taaaaaaact gcctaagaca
    26161 gtaggggagc tcgactcata aatatcctac cgaggacaaa acgtatcatg taaaaagaag
    26221 cgtattgtac tttcgtgtct aggtttgggc gcgtttttct ttttgaaaaa ttccccaagc
    26281 aaaataatta cggaggtgaa cacactaatg agtgaagcaa ttattgctaa aaaagcggaa
    26341 ctagttgacg tagtagctga aaaaatgaaa gctgctgcat ctatcgtcgt tgtagacgct
    26401 cgtggtttga cagttgagca agatacagtt cttcgtcgtg agcttcgtgg aagcgaagtt
    26461 gagtataaag ttattaaaaa ctcaatcttg cgtcgtgcag ctgaaaaagc tggtcttgaa
    26521 gatcttgcat ctgtatttgt tggaccatct gcagtagcat tttctaatga agatgttatc
    26581 gcaccagcga aaatcttgaa cgacttttct aaaaacgctg aagcacttga aattaaaggt
    26641 ggtgcaatcg aaggcgctgt cgcatctaaa gaagagattc ttgcacttgc aactcttcca
    26701 aaccgcgaag gacttctttc tatgctcctt tctgtacttc aagcgccagt gcgcaacgtt
    26761 gctcttgcag tcaaagcggt tgcagaaagc aaagaagacg cggcttaatc ttaagctaca
    26821 cagcgtagcc tagctacgaa aaaaactatt ataaaattta aaacttattt ggaggaaata
    26881 acaatggcat tgaacattga aaacattatt gctgaaatta aagaagcttc aatccttgaa
    26941 ttgaacgacc ttgtaaaagc tatcgaagaa gaatttggtg taactgcagc tgctcctgta
    27001 gctgttgctg cagctgatgc agctgatgct ggtgctgcta aagattcatt cgacgttgaa
    27061 ttgacatctg caggcgacaa aaaagttggc gttatcaaag ttgtacgtga aatcactggt
    27121 cttggtctta aagaagctaa agaacttgtt gacggtgcac cagcacttgt taaagaaggc
    27181 gttgcaactg cagaagctga agaaatcaaa gctaaattgg aagaagctgg agcttcagtt
    27241 actcttaaat aatagagtat cgcaatttga ttgcggaatc cctatttacc agtctttgag
    27301 agcgataagc gattgataga aactgataaa ttgatttggt ttcctgccaa aaattctttg
    27361 gcaggatttt tgttttaata aactttcggg ggtagacact agattgtcta cccctttttt
    27421 tgtgtttcgg aggtaattat gcaaaaggaa ggacagtcat cacgtcaatt cgtgaagagt
    27481 catctcatga ctatcatggg agttgatatt gtaaaagcaa ctcagttagt tgatgaaatg
    27541 gaacaagtag gtttaatccg atttgatgaa tttggaaatg ttggaatttt agtcttggag
    27601 ggacaataat gaagcgtatt accgctaatc aatatcagac atcagagcgt tattataagc
    27661 tccccaaact tttatttgag agtgaacggt ataagaatat gaaactggag gttaaggtgg
    27721 tttactcagt tttaaaagat cggttggagt tgtctttgag caaaggttgg attgatgagg
    27781 atggggctat ttatttgatt tattccaatt caaatttgat ggcactttta ggctgttcaa
    27841 agtcaaaatt actctccatg tgagtttgaa gtaacatttt tagatgatta ccataaaaaa
    27901 cacaactacc cactatttta cgaatcctat cttcaaaacg ttatggaatt ccttgaaagt
    27961 caagacataa agaatggggt tgatgccttt gtagatgatc atcaaaatct cgtttttgtt
    28021 ttatatggac aaggctatcg agccgaggga aaagagggaa tacttacaac ccaagtaact
    28081 gtaaaagctt atgatgaaga caagaaaccg attaacttcg caaatttatt agattcctta
    28141 atcgtctcag aatatcaaat ggaaccgaat ctttgggagg tctcctatga ttgatctcta
    28201 tctaagtaaa aatagccgaa gaaatcaact tcttttagac ttctttcaaa actatggcat
    28261 cgaggtatct tgtcattcag tttctgaaat gacaaaggac aaattaattg agatgatgag
    28321 ctattcttca gattgttttg agtttttatc tccaaattta ttacgtttta agaatcgaga
    28381 taacctaaaa ttaacagatt tcatcgaaat gatattaaaa aatcctgaac taaccatcag
    28441 acttcctctt gcggtttcaa ataagcgagt ttatccaagt ctgaatttgg aagaggctag
    28501 agctctattg ccaagagata cgaaacaatt gatttacatg gcgcaaacac attatttatc
    28561 taactaaagg agaagcaata tggctgaacg attttgg
//
LOCUS       DAVKJJ010000025        28065 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000025 DAVKJJ010000000
VERSION     DAVKJJ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28065
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.025"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(408..1136)
                     /locus_tag="VW144_001123"
     CDS             complement(408..1136)
                     /locus_tag="VW144_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9600578.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1294..2703
                     /locus_tag="VW144_001124"
     CDS             1294..2703
                     /locus_tag="VW144_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9600579.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLTSAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKKEYGNIPWFISENGMGVENEARFIGENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKRS
                     GRWYRKVSDNNGFEVEIEE"
     gene            2721..4016
                     /locus_tag="VW144_001125"
     CDS             2721..4016
                     /locus_tag="VW144_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9600580.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            4021..4329
                     /locus_tag="VW144_001126"
     CDS             4021..4329
                     /locus_tag="VW144_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9600581.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALEWIGEIR
                     "
     gene            4326..4634
                     /locus_tag="VW144_001127"
     CDS             4326..4634
                     /locus_tag="VW144_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9600582.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
     gene            complement(5501..8152)
                     /gene="adhE"
                     /locus_tag="VW144_001128"
                     /gene_synonym="adhC"
     CDS             complement(5501..8152)
                     /gene="adhE"
                     /locus_tag="VW144_001128"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEU9600583.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVSKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
     gene            complement(8452..8862)
                     /locus_tag="VW144_001129"
     CDS             complement(8452..8862)
                     /locus_tag="VW144_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600584.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            complement(8864..9292)
                     /locus_tag="VW144_001130"
     CDS             complement(8864..9292)
                     /locus_tag="VW144_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEU9600585.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKISLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            complement(9338..9637)
                     /gene="yajC"
                     /locus_tag="VW144_001131"
     CDS             complement(9338..9637)
                     /gene="yajC"
                     /locus_tag="VW144_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9600586.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            complement(9754..11730)
                     /gene="tkt"
                     /locus_tag="VW144_001132"
     CDS             complement(9754..11730)
                     /gene="tkt"
                     /locus_tag="VW144_001132"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9600587.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMNEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEVHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            complement(11843..12934)
                     /gene="ulaG"
                     /locus_tag="VW144_001133"
     CDS             complement(11843..12934)
                     /gene="ulaG"
                     /locus_tag="VW144_001133"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEU9600588.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            complement(13046..14719)
                     /locus_tag="VW144_001134"
     CDS             complement(13046..14719)
                     /locus_tag="VW144_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9600589.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALVDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVTLQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLKVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            complement(14898..15602)
                     /locus_tag="VW144_001135"
     CDS             complement(14898..15602)
                     /locus_tag="VW144_001135"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEU9600590.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKTWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            complement(15604..16467)
                     /locus_tag="VW144_001136"
     CDS             complement(15604..16467)
                     /locus_tag="VW144_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEU9600591.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKEIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            complement(16471..17136)
                     /locus_tag="VW144_001137"
     CDS             complement(16471..17136)
                     /locus_tag="VW144_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEU9600592.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSCDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            complement(17153..17638)
                     /locus_tag="VW144_001138"
     CDS             complement(17153..17638)
                     /locus_tag="VW144_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9600593.1"
                     /translation="MNLKQALIDNDSIRLGLEANDWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            complement(17715..17996)
                     /locus_tag="VW144_001139"
     CDS             complement(17715..17996)
                     /locus_tag="VW144_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9600594.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            complement(18019..19476)
                     /locus_tag="VW144_001140"
     CDS             complement(18019..19476)
                     /locus_tag="VW144_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9600595.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAAIVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFIFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            complement(19607..20230)
                     /locus_tag="VW144_001141"
     CDS             complement(19607..20230)
                     /locus_tag="VW144_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEU9600596.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQTKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            complement(20281..21267)
                     /locus_tag="VW144_001142"
     CDS             complement(20281..21267)
                     /locus_tag="VW144_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEU9600597.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQTIIDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNCYSRTFYITINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            complement(21286..22110)
                     /locus_tag="VW144_001143"
     CDS             complement(21286..22110)
                     /locus_tag="VW144_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9600598.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            complement(22085..22456)
                     /gene="rnpA"
                     /locus_tag="VW144_001144"
     CDS             complement(22085..22456)
                     /gene="rnpA"
                     /locus_tag="VW144_001144"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU9600599.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            complement(22605..23795)
                     /locus_tag="VW144_001145"
     CDS             complement(22605..23795)
                     /locus_tag="VW144_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEU9600600.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            complement(23846..24799)
                     /locus_tag="VW144_001146"
     CDS             complement(23846..24799)
                     /locus_tag="VW144_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9600601.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELEQVKDNNQTLKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLTKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYTHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSNLLKVWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            complement(24860..25454)
                     /locus_tag="VW144_001147"
                     /pseudo
     CDS             complement(24860..25454)
                     /locus_tag="VW144_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(25591..26004)
                     /locus_tag="VW144_001148"
     CDS             complement(25591..26004)
                     /locus_tag="VW144_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9600602.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKVEQESGEQFFNLGQVSYQNKKTGLVTRVRTDKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKVEKKKSEEKPEKKENS"
     gene            complement(25982..26443)
                     /locus_tag="VW144_001149"
     CDS             complement(25982..26443)
                     /locus_tag="VW144_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9600603.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTNARGRGYQPMVYGLKSVRITEDNQLVRFHFQFQKGLEREFIYRVEKEKS"
     gene            complement(26406..26666)
                     /locus_tag="VW144_001150"
     CDS             complement(26406..26666)
                     /locus_tag="VW144_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600604.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            complement(26671..27075)
                     /locus_tag="VW144_001151"
     CDS             complement(26671..27075)
                     /locus_tag="VW144_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU9600605.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSGSVQSTFSAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLPVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            complement(27068..27394)
                     /gene="comGC"
                     /locus_tag="VW144_001152"
     CDS             complement(27068..27394)
                     /gene="comGC"
                     /locus_tag="VW144_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HEU9600606.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLSKLKEDGRITEEQAKAYKEYNDKNVGA
                     NRKVND"
     gene            complement(27396..>28065)
                     /locus_tag="VW144_001153"
     CDS             complement(27396..>28065)
                     /locus_tag="VW144_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU9600607.1"
                     /translation="VATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
BASE COUNT         8356 a         5867 c         5052 g         8790 t
ORIGIN      
        1 gattcagaat ttattcaata tattaaaaca atatttgaac aaataaattg ggagattgaa
       61 atttatttct agcaattttt tagaagtcac agtctactat tctaacctca gtatactata
      121 tgtgttacaa gaaaagactc aaaaacttct cgtcagtgta agaatgtttg aaacactatt
      181 taaaactaaa taactataag aagtagatca ggtgaacaat ttcagtacct actctataag
      241 ctatacgaag atatttttca acatgatgga taaaacgatc attcttttaa agccatctta
      301 tttagaaaag agaaacgatt taagcataga tgggaccttc ttgccctgtt ttaaattttt
      361 cttaaactag agtctgtaaa agattggctt cattttcaca ttttctccta ggaggaatgt
      421 cgtaatgcaa aagaattaaa ttcaaataaa tcatagcgat gacgactaat cgagtactca
      481 aacggagttc cgttatccaa ataagccact tgttctactt caaataccgg ttccgttgga
      541 aggagctgca agtaatgttg ctcattttct gaactagaag aagctcgtaa gatttttgta
      601 gcactggcaa tatgcaatcc taattttcct tcaatgtaat tgtaaatcga tttttgtaaa
      661 atatcttcag taatacctgg aataacatcg gtactcatat aagtttgttc cagaacataa
      721 ggtttaccgt caataatacg caggcggtaa atattataaa ctggactctg caaagcgacc
      781 tgtagttttt ctgctaaaaa ttcatttgca aattctaatt tgaagtgaat aatcctcgag
      841 tcaattgtag aatgagcatc ttcagatatt tttgtcagtc cccggatatc tctttctgga
      901 acgattaatt ttctttttga gctgccacga gagagaacaa aggttccctg cccacgtttt
      961 ctaaaaatta agccctcaga aactaacaag tctaaagctt ttttgatcgt cattcgacta
     1021 caggcaaact gtttagctaa tgtcatttca tcaggcaatt gttcgttcat ggtatattta
     1081 ttacaaagaa tcttttcacg aatctcattg taaataaata aatattttgt actcatttaa
     1141 tctggttctc tctataatat tctaatagta gtataacaaa gttcaagaaa tgaggcaaac
     1201 acaagatatg tatcaacaat ttttaaaaat gtctagacaa attttaaaat tatgctatac
     1261 tttatgtata ggataataga aaggaggttg agcatgaaaa aaaagttagt atttcctaat
     1321 ctgttttggt ggggagctgc ttctagcgga cctcagacag aaggtcaata tggaaaagta
     1381 catgaaaatg tgatggacta ctggttcaaa acgcatccag aagatttttt cgataatgtc
     1441 ggacctcttg tagccagtaa cttttttcat acttacaccg aagatttcca cttgatgaag
     1501 gaaattggag ttaattcttt ccgcacttcc atccaatgga gtcgactcat caagaattta
     1561 gagacaggtg agcctgatcc aaaaggtatt gctttctaca atgccatcat tgaagaagct
     1621 aaaaagaacc agatggatct tgtgatgaat ttacatcatt ttgatttacc agtggaactt
     1681 cttcaaaaat acggtggttg ggaaagcaaa catgtagtgg agttattcgt gaagtttgcc
     1741 aagactgctt tcacatgctt tggagataag gttcattact ggacaacttt caatgagcca
     1801 atggtcattc cagaagcagg atacttatat gctttccatt atccaaatct aaaaggaaag
     1861 ggaaaagagg ccgtacaagt catctataat ctaaacctta ctagtgcaaa agtgattcaa
     1921 ctatatcgct cattaggact tgatggaaag attgggatta ttttaaactt gacacctgct
     1981 tatccaagaa gtaattctcc agaagactta gaagcaagtc gatttacaga tgacttcttt
     2041 aacaaagtct tcttgaatcc agctgttaaa ggaactttcc cagaaagatt ggtaaaacag
     2101 ctagagagag atggcgtgtt atggagtcat accgaaaaag agcttcaact gatgaaatca
     2161 aatacggttg attttcttgg agtaaactac taccatccaa aacgtgttca agcacaagca
     2221 aatcctgagg aatatcagac gccctggatg ccagaccaat acttcaaaga gtatgaatgg
     2281 ccggagcgtc gcatgaatcc atatcgtggt tgggaaattt ttccgaaagc catttatgat
     2341 attgctatga ttgtgaagaa agaatatggt aatattccat ggtttatcag tgaaaacgga
     2401 atgggtgttg aaaacgaagc acggtttatc ggtgaaaatg gagttatcga tgacgtgtat
     2461 cgtattgaat tttatgaaga acatttaaga tggctacata aagccattga agagggaagt
     2521 cactgttttg gataccacgc ttggaccgca tttgattgct ggtcttggaa taatgcatat
     2581 aagaatcgtt acggatttat ctccgttgat ttagaaacgc aaaagagaac catcaagaga
     2641 tcaggaagat ggtatcgcaa agtaagtgac aataacggtt ttgaagtaga aattgaggag
     2701 taaagagagg agatattcag atgaaaaact caaaatttat agatcaattt gccacctttg
     2761 ctggtaaact agggaaccaa attcatttaa aaaccctaag agatgcattc gtaacagtaa
     2821 tgccattata tattttggca ggtttaatcg ttcttttgaa caacacggta tttaagtgga
     2881 ttttccaagg ggatacatta acaagattcc aatattgggg aataacaatt gcaaacggta
     2941 ctttaagtat ttcaggtatg attattgctg taatggttgg ttatttctta gctaaaaaca
     3001 gagatttcga aaacccgtta gcagcatcaa tgctatcatt agtttcttta attgtgatga
     3061 tgccaaatac agtttctgta gttcctgacg gagcaaaaga tgcggtaaac atttcaggtg
     3121 ttctttcatt caacaacaca ggtacaggcg caatgttcgc cggggttatc gtagcgatta
     3181 ttgcaacaga attattcatt gaattatcaa acgttaaagc tttacaaatg aaccttggtg
     3241 aaaatattcc accagctgtt agtagatcat ttagcgtatt acttccagtc atgaccgtca
     3301 tctccttatt tggggttgtt tcagcattat tattcaatat aactggaatg aacttaatct
     3361 caatcattac aatctttatt caagaaccaa ttcgtcatat tggtacaagc ttaatcgggg
     3421 tcattattat ttactcttta ggaaatatgt tatggctatt tggtattcac caagcagtta
     3481 tttacagtgc catcctagaa ccattactat taattaacat tactgaaaac atcactgcag
     3541 caaataatgg acaagccatt ccacacatca tcaacttatc acaaatacaa acattcgctt
     3601 taatgggtgg tagtggatct acattatgtt tattaatagc aacattctta gtaagtcgca
     3661 atgctgtttc taaaaacgtg gctaaattat cttttggacc tggtatcttc aatatcaatg
     3721 aaccagtatt attcggttac ccaatcgttt ataacatttc attagctatt ccatttatca
     3781 cagttccagt acttggtatt ttaatcagct acttagcaac agttacagga ttcatgagtc
     3841 ctgcatttat acaagttcct tggactacac cagtattctt aaatgcatgg ttagcaacag
     3901 caggggacgt gagagcagtt ctagttcaat tcatcatctt tgcacttgga gttcttctat
     3961 acattccatt tatcaaagtt aatgacaaag ttgttgaaca agaaatggaa ggttaagatt
     4021 atgaaacaaa ttttattagt atgtaatgcg ggaatgtcga caagtatgct tgttaaaaaa
     4081 atgcaacaaa gtgcgactga acgtgggatt gaaatttcta tccaagcaaa atcaatgaca
     4141 gaagctaaaa aaaatatcca tgaagcagat gtcattctaa ttgggccaca aattcgttat
     4201 gagttacttg ctgtgaaaga aattgcagga aatatccctg tagataccat tgatatgcgg
     4261 gattacggaa tgatgaatgg agcaaaagtt ctggaacaag cacttgaatg gataggagaa
     4321 ataagatgac agaagaaatg gaatatatct gttttcagct cattgccaat agtggagcag
     4381 ccaaatcatc ctttattgaa gccatccaac tagctaaggc aggaaattta aaagaagcga
     4441 aaattaaagt tgaagaagcg gaagactcct tagtagaagc gcacaagatt cactccaatc
     4501 tcatacaaaa agaagctact ggagaaaaaa taggattctc cttgctattt atgcatgcag
     4561 aagaccaaat ggcttcaaca gagattattc agttgctatc taaagaattc atcgatctct
     4621 atcaaaataa ataggaacac tgtcgttccc atctgtcttt attaatgacg actacattgt
     4681 aggaacctcc aaaagttgga caaaatgatt gaaaggattg gatcctgcat tacacaagat
     4741 ccaatccttt tcttttactc tagtttttat aatatgttga atctagaata gtccactgca
     4801 gtttctaaaa aattgctgga aatagatgtt aagctccaat ttatttgttt atatcttatt
     4861 tcagtttact atactagctt attattctag tgaaaaattg aatgagccac tatttattta
     4921 atatgtatga tgagggaatg actcaaaaac ttcttgtcga tataagaata tctgagatag
     4981 tattttaaaa cgaaaggact ataaaagctc atttccttcg cacttatttt atagagatct
     5041 atcattatac tctttagaaa tcaaaaagta acacaccttg tcatcgtttt atcgtaaatc
     5101 tgttaatgag ggggctagtc ttatctgcaa tctcaaatca gtgttttgag ctgaatggca
     5161 ctcgttttct agtttgctct ttgattttaa ttgggcctta gtaaatacta ctcttaaatt
     5221 aaactatagt gaattgaaat aagatgtaaa caactctatc aggaaactaa aattaatttc
     5281 tataaatgtt ttagaagcag atgtgtacta ttctagtttc aatctgctat atattttgtt
     5341 tctattctag tttttcaaaa agggcaaaaa ggcctcaaca tcataaaaag gcatcctatc
     5401 aggtattcaa aaaccttgat aggatgcctt ttatcataga aaaattactg gatctttccc
     5461 tcaaattgag tttttgtctt gcttctagac tgataaacaa ttatttacgg cgtcctggtc
     5521 tttctttgta gccatagtat gcatcttcga tgatttcttg catatggtct accattggaa
     5581 gacgtgggtt agctggtgaa cattggtctt cataagccag gaaggctaat tcacgagaat
     5641 gttctttcca ttctttttcg tcaattcctt ggtctctaaa attcatttgg atcccaatac
     5701 gttcaccgag ttcgtagaca gcttttgcgt aagattcaac cccttcttct ggagtagaag
     5761 ctggaagtcc aagcatgcgt gcgatatctt ggtatttttc atctgcacgg tagtagttgt
     5821 acttaggcca tgttgctgtc ttagctggac gtgtaccgtt gtaacggata acgtatggaa
     5881 gcaagatagc atttgtacga ccgtggattg tgtggaattg cgcaccaatc ttatgggcca
     5941 ttgagtgaga aatacctagg aaggcattgg caaaggccat accagcgatt gttgaagcgt
     6001 tatgcatttt ctcacgtgag tggaagtctg cattcttaac tgagctttcg agattttcaa
     6061 agaccaattt aatggcttga agtgctaaac catcagtgta gtcactagcc atttgtgata
     6121 cgtatgcttc tgtcgcgtga gtcaatacgt ccataccagt atcagcagca acaaatcctg
     6181 gaactgtcaa taccaaagca ggatctacga ttgccacagt tggtgtcaat gagtagtcag
     6241 cgattgggta tttacggttg tttgctttat cagagataac ggcaaatggt gttacttcag
     6301 atcctgtacc agatgtagtt ggaatcgcga tgaatttagt cttcttacca agcaatggga
     6361 acttgaaggc acgtttacgg atatccatga atttttggac aaggtcacgg aagtccactt
     6421 ctggttgctc gtagaagagc cacattactt tggcagcatc cattggagac ccaccaccga
     6481 gtgcgatgat ggtatctggt ttgaaggcac gcataatctc agtaccacgg tttacagttg
     6541 tgatatctgg atccggttct acatccgcaa agatttggta aacaacctta ttgcgacgaa
     6601 ggtccagttg ttcgatgata cgatcaagga aaccaagctc taccatggca tggtcagtaa
     6661 cgatcatgac acgttcaacg tcacgacatt tttgaaggta ttgaattgaa tcacgttcaa
     6721 agtatgtttt tgaaggaagt ttcatccatt gcatgttatt tctccgtctt ccgacttttt
     6781 tgatattcaa gaggttaatg gcactaacgt tatccccaac tgagttgcgt ccgtaagaac
     6841 cacatccaag tgtcaatgat ggcaagaagg cattgtaaac gtccccgata ccaccaaaag
     6901 tagaaggtga gttacagata acacgaatag ctttaacagc tttaccaaat tctttagtca
     6961 attcttcgtc agctgtgtgg atagctgctg agtgtccaag accgttaaat tcaaccattt
     7021 gacgagcctt agtaatacca tcttcacggc tttcagattt caaaactgca ataactggtg
     7081 acaatttttc acgagtcaat ggctcatttt cgccaacttc tttacattct gcagcaagaa
     7141 tgtttgttcc ttctggaact gtaaatcctg cttgttctgc aatccaagtt gctggtttac
     7201 caacgatgtc agcgttcaat tttgcaccag cacagttttt gctgtttgct ttgacgccga
     7261 agcagaactc ttcaagaaga gctttttctt ttttgcttac aaagtaagtg tggtaagatt
     7321 tgaactctgc tacaaattca tcgtaaattt ctttatcaat gataactgct tgttcagatg
     7381 cacagaccat accgttatca aatgatttag acatgacgat atcgtgtgct gcttgacgaa
     7441 tgtttgctga tttttcaaca taagctggaa cgtttccggc acctacccca agagctggtt
     7501 taccacatga ataagccgcc ttaaccatgg cattaccacc tgttgcaagg attgtcgcaa
     7561 caccttcgtg gttcataagg gcacttgttg cttccataga tggttgagta atccattgca
     7621 cacagttttc aggagcacca gctgcgatag ctgcatcgcg gacgatacga gctgcatgag
     7681 cagatgattc ttgtgctgat ggatggaagg caaagacgat tgggttacgt gtcttcaatg
     7741 aaatcaatga tttgaagatt gctgttgatg ttgggtttgt tgttggagta ataccacaaa
     7801 caacaccaac tggttcagca ataagagtca atcctgttac atcgtcttct tcgataacgc
     7861 caactgtctt agtgtggcgc atgttgttta ctacgtgttc acaggcaaac aagttcttag
     7921 ttgctttgtc ttcaaataca ccacgtcctg tttcttcaaa ggcatgtaaa gccaattctc
     7981 cgtgggcatc caaagctgct actgatgctt tggcaacgat gtagtcaact tgttcttgat
     8041 ccaatttacg catttcttca agggcaacta gagctttttg aaccaactca tctacgtgtt
     8101 tttcagcaac gagtttcttt tcctctggtg tcacagtttt tttatcagcc atattttcct
     8161 ccatagcttt caaggaacga tatcctttgt taattttttc acaagtttat tataacgcat
     8221 tatttcaact ttgtaaacag tttcataaac atttcacaaa gaatttttaa actcttgtga
     8281 aacttttctc attttctttt cttatcaagt tttatctttg aagtttgtga aatttatttc
     8341 aaatattttt tatttcacaa acttgcttgg aagagggatt tggaaagaaa gggatttaca
     8401 ggtaagcgct taccttgtga ttctaataat acctcctttg ataaggaggc tttatttttg
     8461 ttgaaaaacg ccttgtttaa aagttccttc ataaacgact tcttgttctg ttgttagttt
     8521 cccttttcct tcagcctgac catttacaaa atcaccttcg taggtccagc cttctttaga
     8581 ttgaaaggta ccttttccgt tgaaggctcc attgttgaag ccacctgtat attggtctcc
     8641 attttggaag gtaatggtac cttggccatt cattttacca cggacaagac tgccatcata
     8701 aacaatcgtt ccattatcga gttttaagac accttttcct ggaatattta gaaaaaagac
     8761 gagtacagca cagaaaacaa tggtaactac tgccaaaagc tctaaacgtg gacgagtcag
     8821 atagacacga tacttttcgt aaaaattcgt aagattttcc accttaactc tccttttcta
     8881 aacgttctaa ccaagcttga cagccctctt gaacacgtcg ataggtttct tcaaaatctc
     8941 ctgtatacca agggtctgga acactttcag atgaaaatga gtaaatctta tcttgacagt
     9001 ctactggaca catctgacgt aagtcagaaa tatttgaagc gtccattccg ataatataat
     9061 caaaggcttc aaaatcttcc ttactaatct gaagcgatat cttgttcttg tcataaggaa
     9121 tctcatactc ttgaaaaatt ccctgagtcc ccttatgaat cggattgcca tgttcccaag
     9181 aggaagttgc tcgactttgg atttcgtagt tatctgtcat tgatttcata acaaactcgg
     9241 ccatagggct acggcaaata tttcccagac agacaaagac taattttttc atcccatttc
     9301 ctttctttta tagaaaacgg gagttcgtga tcccgtctta tttttcaatt gcgccttcta
     9361 gtgaagctgt ttctttcagc ggtaatactg tcttgatagc agctagttca aaagtcaagt
     9421 aaactccatc tacgtcaaga actattgttc ccttctccgt atctacttca tcgactgttc
     9481 cataaagtcc accgattgta atcacttcat agcctttttg tagtttattt aagctttcca
     9541 tacgcttttg agcttgtttc ttttgagaac gttgcataaa gaacatcaag gccatcatac
     9601 ctacaagcat gattaaaaaa gtaatatttg gattcattgt tttctccttt gtcttttaca
     9661 taggactact atatcaaatt tcagctactt ttacaagatt gtaccttact tttctggtaa
     9721 gaaaaaccag agcttacgcc ctgattttta ggattatttc aaatttcgaa caacttttac
     9781 aagattttcg acagtaaagc catattctgc caacactttt ggtgctgggg cagaggctcc
     9841 gaaggtatca atacctagaa cggcaccatc gagaccaaca tatttgtacc agttttgact
     9901 tgcacccatt tcgactgcaa cacgacggcg gactgcattt ggaagaattt cttccttgta
     9961 agctgcatct tgtttatcaa agacatctgt agatggcatg ctgactacgc ggattttttc
    10021 gccttgacta gccaattctt tagcagctga gacagcaaga ttaacctctg aacctgtcgc
    10081 aatcaagatg gtatcaaagt cggctgcatt ttcatataca acataagcac ctttagcaac
    10141 cttgtcgaag tctgttccat cttcaacagt caagttttga cgtgtcaaga caagggcagt
    10201 tggtgttttc tcacttgtca ctgcaaggta ccaagctgct tgcgtttcac gcgcatctgc
    10261 tggacggaaa acatttagat ttggcatagc acgaagacct gctaaatgct caactggttc
    10321 gtgagtcgga ccatcttccc caactgcgat tgaatcatgg gtaaagacat aagtcacagg
    10381 aagtccttgt aaggctgaca agcggacagc tgccttcaca tagtcagaga agacgaagaa
    10441 agttccaccg tatacacgaa gtccaccatg aagggccatc ccgttcaaga ttgttcccat
    10501 tgcaaattca cgaacaccaa actgaatgtt acgattcaag cgattggcat cgtcttgaag
    10561 tccatccgtc ttgatataag tcatgtttga gtgagcaagg tcagctgatc cacctaggaa
    10621 ggttggtaat ttagctgcca caacattcaa ggcatcttga cttgaattac gagttgcttg
    10681 agaaaaacca ttttctaaag ctgggaagtc tgctggagtc acttcgactg gatcacgtcc
    10741 gtcgatgatg gcttctactt ctgcagccag ttctggatga acttctttat aatctgcaac
    10801 taatttagtc caagcttgat aagctgatgc gccacggtct gcaacatgtt ctttgaaatc
    10861 agcatatact tgttctggaa tttcaaatgg ttcgtagtcc caaccgaggg cttggcgagt
    10921 tgatgcagtt tcatctgctc caagaggggc gccgtgtaca gcattagttc cttgtttgtt
    10981 tggagaaccg tatccaataa ccgtcttcac ttcaatcaaa gatggcttgc ctgaagcttt
    11041 tgctgtttcg atagcagcat ggatggcttc caagtctgtt ccattttcaa ccaaggcagt
    11101 atgccaaccg taggcattgt aacggtcacg aacactttct gtaaaggaat cctttgtctc
    11161 accatccaag ttgatatcat ttgaatcata aagaacaacc aacttatcaa gtttttgcaa
    11221 acctgcgtat gaagctgcct cgcttgagac accttccatc aagtctccgt ctccacagat
    11281 aacgtaagta tagtggtcaa agatattgta gccttcacgg ttatatttgg ctgccaagaa
    11341 acgttctgct tgggcaaaac cagtagcagt tgaaatccct tgccctagag gacctgtcgt
    11401 agcatcaatc cctgccgtat gaccaaattc tgggtgacct ggtgtttttg aaccccattg
    11461 acggaagctc ttaatctcat tcatgctgac atcttcaaaa ccagaaaggt gaagaagagc
    11521 ataaaggagc attgaaccat gacctgctga aagaataaag cggtcgcggt taatccaatt
    11581 tggttgagct ggattgatat gaagttgttt tgtaaagagg ctgtaagcca tcggagccgc
    11641 tcccataacc acacctggat gacctgagtt ggctttatta atggcgtcaa tacctagaaa
    11701 acgaattgca ttaacagata gatttgacat agaatttcct ttctattgag gtgattattg
    11761 aatcacacat tctattttac tattatatga aaattatccg tatcatgcaa catacggata
    11821 acctccaaag aatattttta tattatagca aagctttaaa ttgaatgtta gagtcttgtt
    11881 caaaacaatc atcaaaacca cgtggatgat ggtattctac taagtgttga tcttgaggat
    11941 aagtgtactt accgccaact tcccagataa atggatggaa atcgtattgc aagcgatctt
    12001 ttcgcatttt ccaaagttct agaatctcat tagtagaagc catgaagtta gaccagatat
    12061 catagtgaac tgggataatg actttggtac gcagattttc tgccatacga agaaggtcga
    12121 tagatgtcat tttgtcttgg atacctaccg gattttcacc atagttattc aaagcaacat
    12181 caattttaaa gtctttacca tgttttgcaa aatagtttga gaagtgagaa tctgcaccat
    12241 gatagatggt tccacctggt gtttcaaaga tatagttaac agccttttga gccatttctt
    12301 catctgtaac agccaagcca gcaagttcac cgcctgtctc atcagcaccg ttcactggga
    12361 gagttaccaa gcaagtacgg tcaaatgatt ctactgcatg aatcttcata tcttttaatt
    12421 cgatagtgtc acctggttta acaacgatga tacgttcttt tggaacaccc catccttccc
    12481 agattcgtcc acagtggtaa ggaccgataa acttaacatg ctctaactta ggattgttga
    12541 gaattgctgc agctgtgtat gggtcgatat gatcactgtg gaagtgtgaa actaagtaat
    12601 agtctagttc gttgatagca aatggatcga taaccattgg ctgaacacgc aagtttggtt
    12661 gcaacttacg aacacctgcc atatttgcca tttggtgccc acgaaccata tctttcactt
    12721 ttttggttga ttttccacgg tttgaccaaa ggtccatgac aacgttagca ccagctggtg
    12781 tcttaatcca agtaccacag ttgcctagcc accacatggc aaagttgcct tcaggtacga
    12841 cttcttcttc gatttcttcg ttcaaccatg ttccccactc tgggaaagtg gctaaaatcc
    12901 atgactctct tgtaatttct ttgacgtttg gcatatgttg cctcctttgg atataattgt
    12961 tatctttgag attagtatac cacccttcaa aacactaaaa aagaccaaga aaaacacagg
    13021 tttgtgttca tcattggact gtccttcaaa tatcttccaa aaaagcttga actactattt
    13081 cttgatttat aagtgtttga acctcttctt ggaacttact agccaacttg ctgtctacaa
    13141 tataagacga aataagacta gaaagatttt cactgaaact tttgctctta ttacgaaata
    13201 tattgtgttt aagatagtct agcattttca aaatatcttc tgcttcaagg ataggattaa
    13261 cccttaaaat cggaaatctg ctatccaaat catcattagt agtaatcact acatcaactt
    13321 gtgcaatatc ttccacactc ttaaattgtt ctgttgtaaa tacagtgtca atttgctcat
    13381 ttggaaaata aagtttgcat tgtttcagca aaagtctcga aaccgcaaca ccttcatcac
    13441 aaacgagata aacttttttc atattttttt gagacgatgg tgtatacttt aaaaatcctc
    13501 caatatgaat cgtcaaatag gcaatatcat cgtctgttag ccgaataaac catgctcctt
    13561 ctaaaatttc cgcagatttt ctagtgacta aaaataattc tccatacttg gatcgaattt
    13621 gttttgttag aggattttta gaaaaaatac cgtaagtctt tctaaataac aaggctttac
    13681 agtggatcat caaatttcgt aacaaatcat ccttgttctc aatctccatt cggatttgtg
    13741 attcaaaata ccagataaat tcttctaaag caagttttaa ttgccgaaaa tcatcacttt
    13801 ctgcatgaat atccaaatct ttacgatagg agaggaggag aacagctaca agagaaactt
    13861 taagtcctga caaagaaatt tcaaactttt gaaacaaccg ttctcctaat ttcttagaca
    13921 cctgatactc tattcttttt cttatcaaag aaaactcttt ctctatatcc tgatgtcttt
    13981 cttgatactg ttcaacatta tgacagctta acagcaaata aggtagaacc tgcaacataa
    14041 aagttatttc atgatgattt attttcttcc ctaaatcttg ttcaactaaa ggaacctgtt
    14101 ccttaaaaaa ttggttcatt tcaacagaaa gcaaacactc atcatctaac ctctctttca
    14161 tcttatcttc taaaatagat acaaaagtgg cattttcttc cataaaaata tgatatagaa
    14221 gcgattgaag atactgaatt ttattaagag ggtgggcatg aaggtggtat ccctgtgact
    14281 tgctcacttg caaggtcacc tgatattgtt ccaaagttag ttgataacga atgctattca
    14341 aatcattgag aacagtattc ctagatacct ctgttaactc tatcaatttt tcaatcgtaa
    14401 tacgttcttt agaaatacct atccatagta acattatcat catacgttca tgcgcactca
    14461 tgatataatc gtacgaatct acttccgata atagtttaca acaagcatct ctctgctctt
    14521 ccgttaagtg aataccaatt cgtggaatac taatgatgtg aagcgcctcg tcaaccagag
    14581 cagcattgat tttgtcaatg tgataataaa ttttccttct tgactctttc aaatctttcg
    14641 aaatcgccat aatcgttttg gacgtttctt gatccatcaa atattgaagc aaatcacaac
    14701 ttgccttatc cagtaccata ttaaaaaatc ccccaaatct attgatatca cagcaattct
    14761 ttttatttta ccatatatat atcaagcgct atttctaaaa aatctttcaa aattacttca
    14821 actccaactt tactctcaat aaaaatcaaa tgtaaaaaga ggagctaaat ttatcttttt
    14881 ctcctccttc atcgttctta cttttgacca taataagcat ttggtccatg tttacgttgg
    14941 tagtgttttt ctagtatgta ctggggagca ggtccaactc ttggattgat ttgttctgta
    15001 aagcgattca tctttgatac ttcctctagt acgacagagt gataaacagc attctctgga
    15061 tttttgcccc aggtgaatgg accgtgattg cgtacaacaa ttcctggtac ttcaaccggg
    15121 ttaagtccgc gatgttcaaa ctcttctacg ataaccaggc cagtatcttt ttcataggcc
    15181 acttctactt cgtccttggt caaactacgg gcgcaaggga ttgaaccgta gaaataatct
    15241 gcatgggttg ttccgtagaa aggaatatca cgacctgcct gagcccaacc aacagcttct
    15301 gtcgaatggg tgtgaaccac actaccaatt tctgaccaag tcttatataa ttgcacatga
    15361 gttgggaggt cggaagatgg tcttaaatcc ccttctagga tcttaccatc tagatcagtc
    15421 actaccatgt tttcaggtgt caattcgtca taatccacgc ctgatggttt gataacaatg
    15481 acaccgagtt cgcgattgac ttcagataca ttcccccagg taaatttgac aagtccatgt
    15541 tttggcaatg attgattggc atcacagact cgtttacgca tagcattgat tacttgattc
    15601 atcttacatc aaacctgctt tcttaatgag tggatagaga aaagcttgcg cctcttgaat
    15661 ggctgcgcgt gtttcttcta ctgtttcaca attttcagac cacatttcga ttaggaaagg
    15721 tccattataa ttggtttcct ttaaaatatc gaaagcttct tcccatttga cacaaccttg
    15781 cccgaaaggt acatctcgga actggccctt tgaactttct gtcactgcat aagtatcctt
    15841 gagatggaga gctgcgatgg catgatgacc aagataaaac tcactataga tatcattatg
    15901 ccatgcagac acattaccaa tatctggata tacaaagagg aagggagagt caatctcttt
    15961 ttctatagcc aaatattttt cgatgctact gatgaaagga tcatccataa tttcaatagc
    16021 aagtaccacc tgagcttctt cagcccagtc acaggctttt ctcaaatttt tgataaaacg
    16081 ttggcgtgtc tggggtgact tttcctcata gtaaacatcg taaccagcta attgaatcgt
    16141 acgaactccc aagtcttgag ctaattcgat acattttttc atgagttcta gagatttttc
    16201 ctctagaact ggatcttttg atcccaatgg gtagcgacga tggcctgaaa aacagataga
    16261 aggaatacga acaccagttt catagatttc tttgacaact tccaagcgtt cttccttact
    16321 ccagtcaagt cttgctaaac gctcgtcacg ttcgtcaata gacatctcga caaaatcaaa
    16381 gcctaactcc ttggcaaaat ttaaacgttc tagccaagta aagtgtgttg gggttgcctt
    16441 ttcataaatt ccaattggac gtaccatgat ttacccccaa attcgtttga tttcatcctt
    16501 gaaggcacgc gctgctcctg ctggatccgc agcctctgta attccacgac ctgcgataaa
    16561 ggtaaagaca tcaacacctt caaagagttt gagagtatct acatctagac cacctgttac
    16621 agatacacgg aagcccatgt caatgagttt tttaacctta ttaaggtctt tttcacccca
    16681 agtttcacca gcaagaagag catcacaaga ttggtgataa atagcttgtg agatacctgc
    16741 atctagccaa agctgagctt gttcaaaagt ccaatcgcca taaagctcga tctggatttc
    16801 gcctcgttct cctcgttcag tcttgatagc ctttagagct gcttccatag tagggatggt
    16861 tgcacaacag atacaagtca tccagtctgc tccacgaacc gcattatttt tagcaactgt
    16921 tccaccagca tcagcacatt ttgtgtctgc cacaataatc ttatctggga aaaggctacg
    16981 caagacttca gccagttcac ttccaacttg aagcaagcaa acagttccag cttcgataat
    17041 atctacttcc tgaccaacag aaacagctgc tttaatcgct ccttgcaagt ctgaatggtc
    17101 taatgcaact tgtaaattag gtattctttt tgtcatttct ttattcctct ttctaacttt
    17161 ccaaatccaa tccttcgaga taagggctat ccttagattc ttcaatcata gccaagacat
    17221 cttctttagt ctggcaagcc tgtaaacgtg caatagaatc ttctaattca aacagggcaa
    17281 taatttgtgg aatggctaca cttgtgtgaa tttttgaact tgttgctgct agtgccaaca
    17341 aaacagatac ctctttccca tctgaaaata caacaggatt ttgtaaggta atcaatgaaa
    17401 aggcatcact ttgcacacca gcttcaggtc tagcgtgggg catagccata cctggcatca
    17461 agatatagta aggcccatac tcttcagtcg attcaatgat agcatcgtaa tactctggca
    17521 aaattgcccc actttcaatc aagggatcta ctgctacctt gactgcttct ttccaatcgt
    17581 tagcctctaa acctagtcgg atcgagtcgt tgtcaattaa agcttgtttt aaattcatat
    17641 ctgcctcctt tatttaaaga gggagaagga cagaaacgat aactctcagt ttctgtccag
    17701 ccccctccaa ccttttactg tagtgcttga ctgagttttt cggtgatttc tttatcatcc
    17761 atcaagttat caagcccaat taacttccca ttagttcgcc cttccaattc ttgaatcaaa
    17821 tgaagagaag cgattacgat gtcatatcct actgctaaac ctttagcttc accgacactg
    17881 catgaattga ctgtaaaatc tgtttgatta agcttacgga gagcattttc aaccttcatc
    17941 ttgataacca ttgatgaacc cattccattt ccgcacgctg ctaatacttt aaccatttta
    18001 tttctccttt tctagatact aagcttcttc ttgaacttca ccgttgtaat atttctcttt
    18061 atcttttgct ttggcaaatt gaagttgagg aataacaagc aagaagagac acacaagtac
    18121 ataaccaaca ataccaaggt atttgaagat atatccaaat ccaagccatg ggaattcaaa
    18181 gtcgatattt ccatggtagc caccataaga tgccaaatcg agaagggcca cacaaagagc
    18241 tcctagagca acttgaagga cacctgatat aaaggaaagg ataacagccg ctttccatcc
    18301 gccgcgttta tcagcgtaga ccgcaatggc tgcattgtca aagaacactg gtacaaatcc
    18361 tgtaataata agaatcggat ttttaaagac gatgagcaaa acaattgtaa tcaattgacc
    18421 aatcaaacca aagataaatc ctgacaggac agcatttgga gaaccaaatc cataagaagc
    18481 tgcaacgtca accgctggga atgaacctgg caacaatttg tttgaaatac cttggaaggc
    18541 gtttgtcaac tcagatacga acattcggac accttgcatc aaaacgaaca agtaaactga
    18601 gaaggtaaag gctgtttgga taatgtacat aaagaaatct tgtttagcag gattgaatag
    18661 agttcctgaa gtgatgactt ctttattaga cataatgtct ggacccaaga ttaaaagaat
    18721 ggctccgaag aatacgagca tcaaggtagc agatgcaaca actgtatcgt ggaagattga
    18781 gaggaactta ggtaatttaa gattgtctaa actttcttct ttcttaccaa agcgtcctgc
    18841 tactttatct acaaaccaga ttgcaaattg ctgttggtga ccaatcgcaa atccgccacc
    18901 accagtcaag cgttgagttg cctcaacagt catatttgaa ctaactgccc agtaaagtcc
    18961 acagatgata ccaatcgctg ctgtaccgta agcattgcgc aattgtggta ctaagaatag
    19021 aaccataaga gatactattg cagcttgttg taccatgatg tgaccagtaa taaagagggt
    19081 tcttaccttc gtaatctttc gaagagctac gagcaagata tttattccaa aaccaatcaa
    19141 tagagctgta gttgcagttc caacaaaatc tggaaactct gctgcaattt tgttgtttgc
    19201 tgcagcaagt ccaaagtaag ggtcgataac cgctgcacca atttggaatt tgtagttaag
    19261 agctgctaag attggacgaa aggttgtaac caaaccacca gcacccacgt taagcaacat
    19321 ataccctact gttgctttaa caaaccctga aaaaacgtca tgggcaggtt ttttcaaaag
    19381 tgcatatcct atcaacacca ataaacctac gaaaaatgcg ggattctgca aaatattgct
    19441 agaaaaccaa tttagaacac ttgaaacgac ttccataatt gttctccttt tgaattgttt
    19501 ttgtaagttt attatataat gcgaaagcgc aaacaaaaag accaagaatt acacagcgga
    19561 ctgttcactc ttggactgta tgatttatga tgttttacaa attatcttat tttctcgtag
    19621 ctagataata gggaagaatt ttagaataag acttcaataa gccttctacg atttcttctt
    19681 cagacgaatt ttctgtcata ggaacatcta ccttcactaa aacttttcga acttcttgac
    19741 ttctcacctt ctcgcgtaaa gtacgacgct tttcttcatt cgcctcccac cgttgacttt
    19801 gaccattaga gtaggttaga taataaatcc ctttaacggt tggaatgtct aaaactttgg
    19861 tctgcttgcc cagtgtttgc tcatccttct tacgttcgat gaaactgact tccaaagaaa
    19921 ttccaaagtc agtagatgtc ccatacaaac gtaaggccat cataggttct gtcacttgtc
    19981 cgtctctctg tagataaacc caaaaatgtg gtctcaaacg ctgggcctga ttcatccact
    20041 gactagtctg ttggagttgc cattctggat ggcttgcttg aaaggctttg gccagttctg
    20101 taaaagcctt tcgggcctct tgtcctttgt ggcggaacgc cagcatcttc tctcgctctg
    20161 ctcccgcttt atcaggacta cggtactgca aaccagaaaa gtctagatag tctcttatct
    20221 tattcaacat cagtttaacc tcctctagct agcaaaaaat caggtcaacc ctgattttac
    20281 ttattctgta tctacaacga catagcgatt tggctcatca ccttcagagt aactggtcac
    20341 gccatccata cgtgaaataa tacggtggat aatcttgcgt tcactattcg acattggatc
    20401 tgtttgatga ctgcgccctt cttctaaaac acgaatcgcc aatttttgcg cataggtctg
    20461 caagacttct gcacggtgtt caacataatc attgacatta atagtgatat agaaggttct
    20521 tgaatagcag ttgtaaagat aattttgagc caacagttgc aaggccttca agactttacc
    20581 atggtagccg ataatacgac ctggttcgtt agtatcaatt tgcaaattga tgctacgacg
    20641 gttataatca tttgaaagtg tagcctcaac atccatgtca tcaataatcg tttgaacata
    20701 agcagtcaca tccgtagcca cctgggcaat atcataactt tgctcgacct tcaagcccat
    20761 ctctttcaag tcttgatttt cagtttgatc ttgctcagtt tcaaggtcat cagcgcctgc
    20821 ttcttccctc atcgcttcct cgatttgaag ttcatttaaa atctcaatgt gaccagtttc
    20881 ttctaagata gtgctggcat gtctttcatg ttttaagatt tcagccttga cttcatcaga
    20941 aataccttga ccttcttcct ctattttttt aatagcatca accacatgac caaggtcaac
    21001 ggtttcttca ctaaccgtct tcacaggctc gttcaaatca ttgatttttt tcggaacgcc
    21061 ttttactacc tgttgatttg ctttgacaac agtcgtttca ctaatcgctt caatatccac
    21121 ttgggctggt tttttaccaa atagaccaag aaagcctttt ttctccctag aaatgacttt
    21181 gatatgagcc ttcattcttg gaatatctaa ttctttcaat cctttctgga ttgcttcttc
    21241 aacagttgaa cctgtaaata ctaccattac cagattcctc cttatttatt tcgttttctg
    21301 agcctttttc ttggcttttc tttttctatt ttccaaatct ttttgtgcct gtactacggc
    21361 ctcgcgctct gcgataatct tgaatggatt attcaagaaa taggtttgca agacttgata
    21421 agcattagac actgtccagt atagggcgac tccacctggc gcataaactg caaagataaa
    21481 aatcaagact ggaatcccat acatcatcgc agtcgtagcg ccatttcgct cagacaaagc
    21541 tttgttggac aaccaagtac ttaaaaaggt gaatactgct gctaaaatcg gaagaacaag
    21601 ggttgtatcc acactaccaa ggttaatcca taagaaatga cctgtcttta aaaagtcaac
    21661 tcttgatagg gcttggaaca gggccaaaat aaccggcatc tgaatcaaaa tcggccaaag
    21721 agagtctgac tgtctgacac ccatttcttt aaatacttta cgcatttcct gctctagttt
    21781 ggttctgctt tccatatctc gacctggata ttgttctcga agcgccttaa tgcgtggctg
    21841 agcttcctgc attttcctag aagccaccat ttgcacctga aagactggca agaggactgt
    21901 acgaatcaag accgtaaaga gaataatccc cactccgata ctaatatcaa acgataaaaa
    21961 gcgaataatt tccgcaaaga agtaaaccaa tttactccaa aaatcagccg attcggctgt
    22021 aatatcgcta gttaccccat tagtcgcaca ggctgtcatg attaacagag acagtcctag
    22081 caaactagtc aactttagtt tctttttcac tcccatttcc ttcccggtaa atctttgata
    22141 attttaatac atggagtaga tttttctcca tctctgcgta tcccaaggtt tcgactcctt
    22201 ttcgagcaat gacaacaaag tcgacatctt ctaccagact cccttttgca ttctggataa
    22261 tatgccgaat ccgtcgctta atttgatttc tagtgacggc attccccagt tttttgctaa
    22321 ctgatagacc tactcgaaaa tggtttttct ggttttctaa ttggtagacc acaaatttgc
    22381 gattagcaaa acttgtcccc tccttgaaaa tcgccttaaa atctttctct ctttttacac
    22441 gaaagttttt cttcaaaact caactccatc tattaaatta ctactattat accatatttt
    22501 tcaaaaaagc caatcatagc aaggttttgg acaatttcat cagaaaaaga agctggaaaa
    22561 ataactttcc caactccttg tattgaatat ttttttagtt tcacttattt tttcaagcgt
    22621 tcaacgtcac gggcaatgac taattcttca tctgttggaa taaccaagac acggatttta
    22681 gctgcctctg ttgagatgtc tcctgtaacg ccaaagacat tcttttcatc atcaacatca
    22741 caaccaaacc acgaaatccc tgagattaca tcacgacgga aactctctgc attttcaccg
    22801 acacctgctg tgaaaacaat ggcatctgct ccatttagca ctgcaaggta ctgaccgata
    22861 tgtttttgga tacggtcaac atacatttca taagccaagc tagcctcgtg attcccttct
    22921 gctacagctg cttctatatc gcgcatatcg ctagaattag cagaaactcc caaaagacct
    22981 gattcacggt taagaacacg actgatatct tctggtgtgt taaaatcctc tgtatattgc
    23041 attaaataag gaatgatagc tggatcaata tcccctgtac gcgttcccat cataatacca
    23101 ccaagaggag tgaaccccat agaagtgtct acagatttgc cggctttcac agctgtaatt
    23161 gagcctccgt taccaatatg acaggtaatt aacttcaagt cttctaatgg acgtcccaag
    23221 agttttgcag cttctcctgc tacaaactgg tgacttgtac catgagcacc gtatttacga
    23281 accttgtttt ctgtgtaata ttttgttggt agagggtagc gataagcttt ctctggcata
    23341 cttgtgtgga aggaagtatc aaaaacaact acactggtaa tgtctggcaa caattccttg
    23401 aaggcacgaa cacctgctgc atttgccggg ttgtgtagag gagccaacaa actcaactct
    23461 tcaacttttt ctaaaacatc tccctcaaca actgttgatt ctttgaaata ttctccacca
    23521 gcaacaacac gatgtccaac acctgtaatc tcgtcataag ccttgataat atcgaaacga
    23581 atcaagtcat ccaataaaat tttaacagct tgtgtatgat tttcaatatc caaaatttgt
    23641 tgttcagaac ggccgtcaaa ttttacagtt gaaattgaat ctttcaaacc gatacgttca
    23701 atcaaacctt tcgccaatac tttttcttct ggcattaagt ataattgcca tttcaaactt
    23761 gaacttcctg cattgattgc aattgttttt gtcataacat gatacccctt tttaagcgtt
    23821 ttcaactatt ataacaaaat ctattttata tttcagtacc ttgagtccat ttttgaaaat
    23881 tttctttaaa tttcattaaa acacttgcat cttgcaagct agcaagtgga taaacaaaag
    23941 gctctactgc tatttcactt ttcttctgta ggataaaaat agtcttagat tgtttagcat
    24001 tagcaaagag attttcaggt aaactaatca tagcaaccag acttgcctcc tctttcaacc
    24061 agactttcaa caaattactt tgaggactgg tcaacaaatc actcggagct agaaaaatag
    24121 cgtatccgtc tgacttgagg tacttaagtc cttgttccat gagcaagtga tgggtgtaag
    24181 tatgttcttg actagaagca acttgatggc gcgacgcaac ggcatcatca ggataatagc
    24241 caacaggcaa gtcgctgatg accacatcac tttctttgag catttgtgga cgaacggcat
    24301 ctccttggac aaagccagcc tgcaaaccaa ttacatctgc catgctagct gccaaatcaa
    24361 tcagcaaatc atccacttcc attcccaaat aatccacctt tttagtaagc gaggtcaaga
    24421 aagtagcgcc caaaattccc atcccagaac ccatttcgag gatagtaatt tcctcctctt
    24481 taaacagctc ttccacaata aacaccaaaa gtaaagcaat agcatccggt gtaaactggt
    24541 gattggcctg caagggttct gtttgcccag ccttcatcaa gagaaactgg taggtcttga
    24601 gccattcttc tttgcgtagt gctaaacgct taagggtctg attgttgtct ttgacctgtt
    24661 ctagctcagt ctcaccatcc agatagatgc tgttttgctc caccaaggcg tcataaaagt
    24721 tggtcgccaa atcactttgg atgacttgga cattctctag taaataggta taagcttgtt
    24781 caattttttc aaaatccata ttcctatctt atcaaatttc ccttcttttt tccattctta
    24841 tagaaaaatc actccctgac taggcgagtg atttttgggc tactattaaa aagaattctg
    24901 agtgatttcc ttgaaacagg tcttcagcgc tatagagaat ctgactatgc acagatgaaa
    24961 aaccatgctc aattagcttg ttttccagtt cagctaaatc aaatccatga tgattagctt
    25021 ctgtcttggt aaaatcagca atgatgagtt tcccatcttc cttcaaatgt tgatgaaaca
    25081 gtgagagagc cgcatccaaa tcaggcatat gatgaagaac ccgactaaca gcaaggcaat
    25141 caaactcttt ctccaaggga tttttcggta aatcttgctc caaaaactgg atattcttga
    25201 ttgcttgctg ctccactttc aaacgagctt gctccaacat tttctcagaa atgtctacaa
    25261 gagtgactga cttagcctgc ttggctaggg gttaggggca aggctaatag acccgtgcca
    25321 ccaccgaaat ctaaaatttc tttgtatgat agaagatcaa tctgtttctc ggctgcttga
    25381 cataccaagt ttgcgaggaa gatattttta gggaaatcga aagtttctgc tttgtggtta
    25441 aaatcatgtt tcatatcttt aatttagcac aaagtatagt aaactgaaat aagatataaa
    25501 caaatgaatt ggagcttaac atccatttcc agcaattttc tagaaactac agtggactat
    25561 tctggattca acgcattata gttgaattga ctaggaattc tctttctttt caggcttctc
    25621 ttctgatttt ttcttctcca ctttttcgct tgaatcggtc gctacctctt cctttttatc
    25681 tcttttctct tctttgattt tgactgaagg aaacagaaac tcatattggc tcttatccgt
    25741 acgaaccctc gtcactaagc cagttttctt gttttgatag cttacctgac ctagattaaa
    25801 aaactgttcc ccactttctt gctcaacctt atctttggtt cgtttagcca tagcaaaagc
    25861 aaccaatttt tctttattca aagcatagtc ttgatagtgg gcgacttgtc ggttcaaata
    25921 aaattgcaac aaaagactaa agatggctgc tatggtgact gcgtagagga gaacacctgc
    25981 cttaactttt ttctttttcc acacgataga tgaactccct ttctaagcct ttttggaact
    26041 ggaaatgaaa gcgaaccagt tgattgtcct ctgtaatccg tacagatttg agtccataaa
    26101 ccataggctg ataacctcga ccacgagcat tcgttttacg gaaatcatct gactttgact
    26161 taccgatggc gatgtccttg ccatcttgct tcatgtatag gcgattgcct tctacttttt
    26221 cgaactgcga acggtctaat tctacctcaa gttggtccac aaacaagagc cactcctttt
    26281 gctcgctttg ttgctggtag cgaacttctg aaatgaggag ctgactcata gcttgaaaaa
    26341 ggagtaatcc cccactgatg acaatgaggg caagcaggga ttctaacaag gtaaaagcct
    26401 tgaccttatg gctctttgat tgccaacaac tgttctgaac catggtagac ctccaatcct
    26461 ttttcactag aaaatacctg aatctcaact ccgttgatgc ttacctgatt ttgccccgtc
    26521 tgcagggcca tcttagctac cctcaagact tcttcctttt gcaagatttt tgcttcctct
    26581 tgcctatttt tttgaatttg tcccaaaagg agggtcgcaa tgctggcaaa gatagctaga
    26641 gcgactactg cttccagtaa aatcactgcc ctaatttttt gtttccttaa tgcgtttaat
    26701 ttttccattt cctagatata attgatagcg aatcgctcct ttactggtct gaaattcaac
    26761 cttagccagg gacgaattgc ccccagctcg gtcaaatgta atactttggc ctgatggggc
    26821 ctgaattcct ttagggactg gcaacttttg actgccattg ctaagcgtct gcccatctaa
    26881 gttcagacta gtcttttgct gactggctac actgcgtttt tgggtttccc gatagagttc
    26941 ttcaaactcc ataaagaaaa tctgttcctc taccgctgaa aaagtggact ggacagagcc
    27001 ggacaagccc aaggcaagga tactcacaag tcccaaaacc aagagacttt ccagcatggt
    27061 aaaggcctta atcattgact ttacgatttg ctcccacatt tttatcattg tattctttat
    27121 aagctttagc ctgttcttcc gtgatgcgtc catcttcttt taacttgctt aggctagcat
    27181 cttcattctt ttccaagcta taaagttctg cctggctttc caccacctta acaacagctg
    27241 cttttccttt gtcattgact gcttcttttt gcttggtcag attaggtaca aagagcaaga
    27301 aaagcacgct gataatcagc aagaccacca acatctccac caatgtaaaa gctttaacct
    27361 tagctttttt caagaatgtc atcatttttt tcattttaaa aatttacctc catattttga
    27421 tacatgggca tgagcattgc cgcataaagt aaaacgataa tcagtgccac aaagataaaa
    27481 accagtggct gcaccaaatt catggtgcgg ttgactcggg taaaaaaggc ttcccaagtt
    27541 ttttcagcat agatttccaa ctcactaccc agcttggact taacttcccc atactctatg
    27601 atgagactca attccttcct aaagaaagga taggttccta tcgtctgaga aaattcacgg
    27661 ccatttttca gggtttgagc cagatcttga ccgatttctt taaagagctg ggaaccttgt
    27721 tcctgcatca tttgaaaaat ctgcgtcagc tccattccct gtgaaatcat attcccccat
    27781 tcacgtgcat aataggctgt caagtaggtc tgcacaaaga ttccaataaa gggaaggcgt
    27841 gctaagatag aaaagacact catcttagaa cttcttttat aaaaagttag tgctaaaagg
    27901 gcaagcacgg aaacaagccc taccatgcct agaaaaattt gtggcagatt accgataatt
    27961 cgggtggcaa tattgctact atccagttgt gggagcaggt aattccgtag ccccagcata
    28021 attaagagaa gaaaacccag caaaatcaaa ggataggtcg ctact
//
LOCUS       DAVKJJ010000026        27638 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000026 DAVKJJ010000000
VERSION     DAVKJJ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27638
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.026"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            111..545
                     /locus_tag="VW144_001154"
     CDS             111..545
                     /locus_tag="VW144_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9600608.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            557..1747
                     /locus_tag="VW144_001155"
     CDS             557..1747
                     /locus_tag="VW144_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEU9600609.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELAHHDLGFLYTPSCMAEYKINGDGEAREAALKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            1758..2249
                     /locus_tag="VW144_001156"
     CDS             1758..2249
                     /locus_tag="VW144_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9600610.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            2264..3043
                     /locus_tag="VW144_001157"
     CDS             2264..3043
                     /locus_tag="VW144_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9600611.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            3030..3848
                     /locus_tag="VW144_001158"
     CDS             3030..3848
                     /locus_tag="VW144_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9600612.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            3848..4141
                     /locus_tag="VW144_001159"
     CDS             3848..4141
                     /locus_tag="VW144_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9600613.1"
                     /translation="MDTILFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIQDDLVFLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            4163..6064
                     /locus_tag="VW144_001160"
     CDS             4163..6064
                     /locus_tag="VW144_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEU9600614.1"
                     /translation="MKEERRQFFERIDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWAYMLNRQEYLLQFMIGYVVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYYFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYRELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEMLSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYIFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            6124..7125
                     /locus_tag="VW144_001161"
     CDS             6124..7125
                     /locus_tag="VW144_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9600615.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANKRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(7235..7570)
                     /locus_tag="VW144_001162"
     CDS             complement(7235..7570)
                     /locus_tag="VW144_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600616.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(7581..7823)
                     /locus_tag="VW144_001163"
     CDS             complement(7581..7823)
                     /locus_tag="VW144_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600617.1"
                     /translation="MKKEKRQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(7834..8375)
                     /locus_tag="VW144_001164"
                     /pseudo
     CDS             complement(7834..8375)
                     /locus_tag="VW144_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
     gene            complement(8391..8585)
                     /locus_tag="VW144_001165"
     CDS             complement(8391..8585)
                     /locus_tag="VW144_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9600618.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            8751..9701
                     /gene="rsmH"
                     /locus_tag="VW144_001166"
     CDS             8751..9701
                     /gene="rsmH"
                     /locus_tag="VW144_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU9600619.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLIRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            9713..10030
                     /gene="ftsL"
                     /locus_tag="VW144_001167"
     CDS             9713..10030
                     /gene="ftsL"
                     /locus_tag="VW144_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEU9600620.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            10034..12286
                     /gene="pbpX"
                     /locus_tag="VW144_001168"
     CDS             10034..12286
                     /gene="pbpX"
                     /locus_tag="VW144_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEU9600621.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGVT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQATA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKVEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            12288..13268
                     /locus_tag="VW144_001169"
     CDS             12288..13268
                     /locus_tag="VW144_001169"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU9600622.1"
                     /translation="MFISLSAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDMLSVFGYQVHLGIFYIVFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            13832..15937
                     /locus_tag="VW144_001170"
     CDS             13832..15937
                     /locus_tag="VW144_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9600623.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRTTGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            complement(16233..16715)
                     /locus_tag="VW144_001171"
     CDS             complement(16233..16715)
                     /locus_tag="VW144_001171"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEU9600624.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(16810..18294)
                     /locus_tag="VW144_001172"
     CDS             complement(16810..18294)
                     /locus_tag="VW144_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEU9600625.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDATRAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            18447..20057
                     /locus_tag="VW144_001173"
     CDS             18447..20057
                     /locus_tag="VW144_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEU9600626.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIATIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLVRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVEMTD"
     gene            complement(20188..21618)
                     /locus_tag="VW144_001174"
                     /pseudo
     CDS             complement(20188..21618)
                     /locus_tag="VW144_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398391.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            21773..23218
                     /gene="cps4A"
                     /locus_tag="VW144_001175"
     CDS             21773..23218
                     /gene="cps4A"
                     /locus_tag="VW144_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HEU9600627.1"
                     /translation="MSRRFKKSGSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGIDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAILQKLTST
                     EALKNYSTIIDSLQDSIQTNMPLETMINLVNAQLESGGNYKVNSQDLKGTGRTDLPSY
                     AMPDSNLYVMEINDSSLASVKTAIQDVLEGR"
     gene            23220..23951
                     /gene="cps4B"
                     /locus_tag="VW144_001176"
     CDS             23220..23951
                     /gene="cps4B"
                     /locus_tag="VW144_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HEU9600628.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLTEAYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTPDVLDKLEKNRIPTLNNS
                     RYALIEFSMNTPYRDIHSALIKILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QVNSSHVLKSKLFGERYKFMKKRAQYFLERDLVHVIASDMHNVDSRPPHMAEAYDLVS
                     QKYGEAKAQELFIDNPRKIVMDQLI"
     gene            23960..24652
                     /locus_tag="VW144_001177"
     CDS             23960..24652
                     /locus_tag="VW144_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein CpsC"
                     /protein_id="HEU9600629.1"
                     /translation="MKEQNTIEIDVFQSLKTLWKYKLIILLVALVTGAGAFAYSTFIV
                     KPEYTSTTRIYVVNRNQGDKPGLTNQDLQAGTYLVKDYREIILSQDVLEKVATNLKLD
                     IPAKTLTSKVQVTVPADTRIVSISVKDKQPEEASRIANSLREVAAEKIIAVTRVSDVT
                     TLEEARPATTPSSPNVRRNTLVGFLGGAAVTVITVLLIELLDTRVKRPEDVEDVLQMP
                     LLGVVPDFDKMK"
     gene            24662..25351
                     /locus_tag="VW144_001178"
     CDS             24662..25351
                     /locus_tag="VW144_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEU9600630.1"
                     /translation="MPTLEISQVKLELAKKAEEYYNALCTNLQLSGDDLKVFSITSVK
                     AGEGKTTTSTNIAWAFARAGYKTLLIDADMRNSVMSGVFKSRERITGLTEFLSGTTDL
                     SQGLCDTNIENLFVIQAGSVSPNPTALLQSKNFSTMLGTLRKYFDYIVVDTAPIGIVI
                     DAAIIMQKCDASILVTNAGETKRRDLQKAKEQLEQTGKSCLGVVLNKFDTSVEKYGSY
                     GSYGNYRKQKK"
     gene            25366..26727
                     /locus_tag="VW144_001179"
     CDS             25366..26727
                     /locus_tag="VW144_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9600631.1"
                     /translation="MSEKLAKPSVAIVQSFLVILLTYLLSAVRETEIVSTTAIVLYIL
                     HYFVFYISDYGRNFFKRGYLIELVQTLKYILFFALAISISNFFLEDRFSISRRGMIYF
                     LLLHVFLVYVLNLFIKWYWKRAYPNFKGSKKVFLLTATSHVEKVLDRLIESNEVVGEL
                     VAVSVLDKPDFQHDYLKVVAEGEIVNFATHEVVDEVFINLPSEKYNIGELVSQFETMG
                     IDVTVNLNAFDWARNKQIREMAGLNVVTFSTTFYKTSHVIAKWIIDIVGSLVGLILCG
                     LVSIVLVPLIRKDGGSAFFAQTRIGKNGRHFTFYKFRSMCVDAEDKKRELMEQNTMQG
                     GMFKVDDDPRITKIGHFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYEHYTPEQK
                     RRLSFKPGITGLWQVSGRSEIKNFDEVVKLDVAYIDDWTIWKDIEILLKTVKVVLMKD
                     GAK"
     gene            26731..27180
                     /locus_tag="VW144_001180"
     CDS             26731..27180
                     /locus_tag="VW144_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014567579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--LPS N-acetylglucosamine
                     transferase"
                     /protein_id="HEU9600632.1"
                     /translation="MKICLVGSSGGHLTHLYLLKPFWKDKERFWVTFDKEDTRSILGN
                     ETFYPCHYPTNRNLKNLIKNTVLAFNILRKERPDVIISSGAAVAVPFFYLGKLFGAKT
                     VYIEVFDRIDAPTLTGKIVYPVTDKFIVQWEEMKKVYPKAINLGGIF"
     gene            27180..>27638
                     /locus_tag="VW144_001181"
     CDS             27180..>27638
                     /locus_tag="VW144_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug MFS transporter"
                     /protein_id="HEU9600633.1"
                     /translation="MIFVTIGTHEQQFNRLIKEVDRLKGEGFIQDDVFIQTGFSDYEP
                     VYCQWKNLISYDEMNRYIDEANIIITHGGPATFMAVIAKGKIPIVVPRLKKFGEHVND
                     HQMEFCSKILSEGYELSLINDISELQYSLKQEKKTKFYSNNTCFLKKLSKT"
BASE COUNT         8300 a         4871 c         6250 g         8217 t
ORIGIN      
        1 taatgaaagc gctaatattt ttttaaaaaa actattgaca agataaaaat aaaggattac
       61 aatggggaat ataaagtaaa ccggtaaacc taaacaagaa aggagaaaag atgaaaattg
      121 tacttgtagg gcatggacat tttgctacag ggatttatag ttctttacaa ttgattgcag
      181 gcaatcaaga aaatgtggag gcgattgact ttgtggaagg aatgtcagca gatgaactca
      241 agcaaaaaat cttacttgca atttcaaatg aagaagaagt tttaatccta agtgatctct
      301 tgggaggatc gccattcaag gtttcttcta ccataatggg agaaaatcca gccaagacaa
      361 tgaatgttct ctcgggtttg aacttagcca tgttaatgga agcagtcttt gctagaatgg
      421 ctcatagctt tgatgaggtt gttaataaat cagtagtggc ggcccagggc ggagttgtaa
      481 atggtaaaga attgttttca acggatgcag aggaagagga agaagatttc gaatcgggta
      541 tttaaagggc aaaagaatga taaaaaaggt tacgattgaa aaaataaaat cgcctgagcg
      601 cttcttagaa gtaccacttc tgacgaaaga agaagtcggc caggcaatcg ataaggttat
      661 tcggcagtta gaactcaacc ttgactattt caaggaagat ttcccgacgc cagctacctt
      721 tgataatgtc tatccaatca tggataacac ggaatggacc aatggtttct ggacaggaga
      781 actgtggttg gcttatgaat acagtcaaca ggatgcattt aaaaacatcg ctcataaaaa
      841 tgttctttct ttcctggatc gtgtcaataa gagagtagaa ttggctcacc atgatctcgg
      901 cttcttgtac acaccgtctt gtatggctga atataagata aatggagatg gagaggctag
      961 agaagcagcc ttgaaagctg cagataagtt gattgaacgc tatcaagaaa aaggtggttt
     1021 tattcaagct tggggagact tgggcaagaa agagcattac cgtttgatta tcgactgctt
     1081 gctcaatatc caactcttat tctttgctta tcaagaaaca ggtgatcaaa aatactacga
     1141 tattgcagaa agccatttct atgcttcagc taataatgta atccgtgatg acgcttcgtc
     1201 cttccacacc ttctattttg atcctgagac aggtcaaccc tttaaaggtg taacgagaca
     1261 agggtatagt gatgattcat gctgggcacg tggtcagtca tggggagtct atggtattcc
     1321 tttgacttat cgtcacttaa aagacgagtc ctgctttgac ttgtttaagg gtgtgaccaa
     1381 ttatttcttg aatcgtctgc caaaagatca tgtgtcctat tgggatttga tttttaatga
     1441 tggtagtgat caatcacgag attcttcagc aacagctatc gccgtctgtg ggattcatga
     1501 aatgctaaaa catctcccag aggtggatgc tgacaaagat atttataaac atgctatgca
     1561 tgccatgctt cgttccttga tcgaacatta tgcaaatgat caatttaccc ctggtgggac
     1621 aagtctcctc cacggtgtgt actcatggca ttcaggtaaa ggagtggatg aaggcaatat
     1681 ctggggtgac tactattacc tagaagccct tatccgtttc tacaaagact ggaacctata
     1741 ttggtaggag gagaaatatg acaatgccaa atattattat gacccgtatc gatgaacggt
     1801 tgattcatgg acaaggacaa ctttgggtaa aatacctagg ttgtaatacg gtcattgttg
     1861 ccaatgatga agtaagcacg gacaagatgc aacaaactct tatgaaaaca gttgtgccag
     1921 actcagttgc catgcgtttc ttccctttgc aaaaggtgat tgatatcatt cacaaggcta
     1981 atcctgctca aacgatcttt atcgttgtaa aggatgtaaa ggacgcttta accttggtag
     2041 aaggtggtgt ccctatcaaa gaaatcaata ttgggaacat tcacaatgct actggtaaag
     2101 agcaagtgac acgctccatc ttcctgggtg aagaggacaa ggcagccctc aaggaattga
     2161 gccaaactca tcaagtaaca tttaatacga aaacaactcc aacaggaaat gatggagctg
     2221 ttcaagtcaa cattatggac tatatttaaa agaggagatc gttatgtcga ttaatgtatt
     2281 tcaagcgatt ttaattggat tatggacagc tttctgtttt agtggaatgc tgttaggaat
     2341 ttacaccaat agatgtattg ttctgtcatt tggtgtcgga attattctag gtgatctgcc
     2401 tactgctctt gcaatgggag ctattggtga attggcttat atgggattcg gtgttggtgc
     2461 tggaggtact gttccaccaa acccaatcgg acctggtatc tttggtacct tgatggctat
     2521 cactagtgct ggtaaagtca gtccagaagc ggctcttgcc ctctctactc cgattgctgt
     2581 ggcgattcaa ttcttacaaa ctttcgccta cactgtacgt gctggtgcgc ctgaaacggc
     2641 gatgaagcac ttgaaaaacc ataatttgaa gaaattcaag ttcactctaa atgcaacaat
     2701 ttggttgttt gcctttattg gatttacctt gggttgcttg ggtgcccttt caatggatac
     2761 cttgttgaaa ctcgtagact acattccacc ggtattactt acaggtttga cagttgctgg
     2821 taaaatgctc ccagctatcg gttttgcgat gatcttgtca gtgatggcta agaaagagtt
     2881 gattcccttt gtcttgttgg gatatgtttg tgcagcttat ctaaacatcc caacaattgg
     2941 tattgcaatt gtaggtacta tctttgcttt gattgaattt tataacaagc caaaaacagc
     3001 ggatcatgtg gtagaggagg aagcacacga tgactggatc taacaaatta acaaaacgtg
     3061 attatcttaa aacgtctttg cgggcattct tttgtcaaaa tggatttaac tatagtaact
     3121 atcaagggtt gggatatgcc aatgtgatgt atcctgcttt gaaaaaacac tatggagagg
     3181 atcaggaagg tttctaccaa gccttggaag aaaactgtga attctataat accaacccac
     3241 acttcctgcc ttttattacc agcttgcatc ttgtaatgtt ggaaaatggc cgtccggcaa
     3301 aagaaacacg tagcatcaag atggccttga tgggaccatt ggcaggtatt ggggattctc
     3361 tttctcaatt ctgtttagct ccattgttct caaccatcgc agcttcgttt gctcaagaag
     3421 gcttggttgt cggtccaatc ttgttcttcc ttgcgatgaa tacgatttta acagcgatta
     3481 aattgtcaac tggtctgtat ggatacaaac taggaacaac tgtgattgat aaactaagcg
     3541 aacagatggc aacgatttct cgtattgcca atattatcgg tgtaaccgta attgctggtt
     3601 tggcagcgac atctgttaag attatggtgc cgattacctt tgctgcaggg gaagttaaag
     3661 cagacgctaa acaaagtatc gtaagtattc agggaatgct tgataaggtt gctccagctc
     3721 ttctaccagc cctatttaca cttttagttt actacttgat caaagaaaag aaatggacaa
     3781 catataaact cgttatttta acagttatca tcggaattat cggaagctgg cttaagatta
     3841 tagcttaatg gatacaatat tgttttatgg aatagtgatt gtcttggcag tgagtcccct
     3901 tttactgtca agctttcatt ctattcgtca gcaaaagttg cttcgcaaac agatggagca
     3961 acgacaagag tatttagctt ctttaacatc tggtgatgaa gtgttgttat tgtctggaat
     4021 tcatggaaaa atcatttcta tccaagatga cttggtcttc ttgcagattg caaaaggtgt
     4081 ggtcatctat gtagaaaagg aaagtgtaat gggaaagaca aaagaactgc tttttaagta
     4141 gttctttttc tggtaataga taatgaaaga agaaagaaga caattttttg aaagaatcga
     4201 tggaaaccaa tgtcgtgatt atatcttgtc ccactgttca aaagactatg agaaagtcaa
     4261 gagttccctt gaacgcttga tggacaatcg ttttatgttt gatagtcctt gggatatgga
     4321 gccttgttca aaaatccatc aaatccagcc gatggtatgg gatcaagtat ttgaagatga
     4381 tccagaatgg gcttatatgc tcaatcgaca agaatatctc ttgcagttta tgatagggta
     4441 tgtggtagaa ggagataagg actatattca aaagtgcaag ttctttctat ttgattggat
     4501 tgagcaggtg agagaatttt ctcctcaatc cttgatgact agaaccttgg atacgggtat
     4561 tcgttccttt acttggttga aactactctt gctcctcttg aaatttgact tgctagagga
     4621 gaaagaacta gagaaaattt tggtcagtct agaaaagcag attgacttta tgaaaagcca
     4681 ctatcgcgcc aagtacaccc ttagtaactg ggggatttta caaacaattc cgatgcttgc
     4741 tatctattat ttcttttcag ataagatgga cctagaagaa gcttaccatt ttgcttcaga
     4801 ggagttgaaa cagcaaattg agacacagat tttaggagat ggaagccagt ttgaacagtc
     4861 gattctctat catgtagagg tttataaagc cttgctggat ttgtgtctct tgcttccaga
     4921 cttgcaagat agttaccgag agttgctgga aaagatggcg acctatattc aaatgatgac
     4981 aggcttagat ggacggactt tggcttttgg tgatagcgat tctacagaaa cgacagaaat
     5041 gttgagcctg tctgctgtgg ttttgaacaa ggaagacctt cttaacggtc tggatgttaa
     5101 agtcgattta cttagcctct tgttcctggg gcgagaaaag gtcaagcgac tgcaggaatt
     5161 tgaaaggaga gcttggcagc ctaagtccat gatctttgaa gactctggac atgtctgcat
     5221 taaggatgaa catcgttata tatttttcaa aaatggtccg ctaggaagtg cccatagcca
     5281 tagtgacgag aatagttttt gcttacagta tcaaggccaa cctattttca tagatgctgg
     5341 gcgttattct tatcgggaga tatatgaacg ttatctctta aagagtgctt ggagtcattc
     5401 gacctgcatt gtagatggga aagctccgga aagaatcacg ggatcctggg aatatgaata
     5461 ctatcctcac tccctgtttt gtcaccataa agaaagggag ggagtgcatt atattgaggg
     5521 ggcttattgg tcagcagaac ctgatttgcc ttatcttcac aagagaaaaa tcctcatgtt
     5581 ggtagaggat gtctggctct tggtagatga catcaggtgt caaggtcagc atgaggcgtt
     5641 gactcagttt atccttgaca aggatgtgac ctatcaagat gggaaaatca atcagttgag
     5701 actatggagt gaagttgatt ttgatttgga agataccatc atttctccta aatacaatga
     5761 gcttgaaaga agtagcaaac tcaccaagcg ccaattcttt gagaatcaga tgctggatta
     5821 taccatcatt gcgcatgaga gttttgaaat catccgtcat tctgtctacc agacagatga
     5881 tcgtgaagtg gaaaatgctc tggcttttga agtgaaaaat gacgaaacag acaagctgat
     5941 tctgttatta agcgaggata ttggtgtagg tgaaaaattg tgcctcgttg acggaacaaa
     6001 aatgcgtgga aaatgtctag tatatgataa aataaatgag agaatgattc gcttgcagtg
     6061 ctagaaatag gcattttgaa tagtgaatat gttataataa gtattagtag gaggtgtttt
     6121 agattggaga agaaactgac cataaaagac attgcggaaa tggctcagac ctcgaaaaca
     6181 accgtgtcat tttacctaaa cgggaaatat gaaaaaatgt cccaagagac acgtgaaaag
     6241 attgaaaaag ttattcatga aacaaattac aaaccgagca ttgttgcgcg tagcttaaac
     6301 tccaaacgaa caaaattaat cggtgttttg atcggtgata ttaccaacag tttctcaaac
     6361 caaattgtta agggaattga ggatatcgcc agccagaatg gctaccaggt aatgatagga
     6421 aatagtaatt acagccaaga gagtgaggac cggtatattg aaagcatgct tctcttggga
     6481 gtagacggct ttattattca gccgacctct aatttccgaa aatattctcg tatcatcgat
     6541 gagaaaaaga agaaaatggt cttttttgat agtcagctct atgaacaccg gactagctgg
     6601 gttaaaacca ataactatga tgccgtttat gacatgaccc agtcctgtat cgaaaaaggt
     6661 tatgaacatt ttctcttgat tacagcggat acgagtcgtt tgagtactcg gattgagcgg
     6721 gcaagtggtt ttgtggatgc tttaacagat gctaataagc gtcacgccag tctaaccatt
     6781 gaagataagc atacgaattt ggaacaaatt aaggaatttt tacaaaaaga aatcgatccc
     6841 gatgaaaaaa ctctggtatt tatccctaac tgttgggccc tacctctagt ctttaccgtt
     6901 atcaaagagt tgaattataa cttgccgcaa gttgggttga ttggttttga caatacggag
     6961 tggacttgct tttcttctcc aagtgtttcg acgctggttc agccctcctt tgaggaagga
     7021 caacaggcta caaagatttt gattgaccag attgaaggcc gcaatcaaga agaaaggcaa
     7081 caagtcttgg attgtagtgt gaattggaaa gagtcgactt tctaaaatga aggaaaatga
     7141 cttgcaatct ctgttaagaa ataaaataat cccacctaga acaagctagg tgggattatt
     7201 tgcctatgaa atgagaaatt atgggagcaa gctcctaaat caactgtttt tgatctactt
     7261 ctttaactac ttgataaaag ttatagaagt aggccaaact tgaaatgatg gttacgacta
     7321 ggaatattga aaatttccat tggacagggt tggttaaaag ttgtggaaag gatatgagga
     7381 gaaagaagag ggctgcgttg aggacaggta tccgttttga ttgtattttc tcaagtcctt
     7441 tattgagcgc aggaagaaag aggagtagga gtagtaaaac tgtatgagaa acagctcctg
     7501 aaataagggc aaagaaaagg aaaatactga taaaaacatg aatgatcagt agtctagcta
     7561 gtgatttcat aaggtacctc ctaatcctgg tcttttttag ctcttgcaat acgaagtgag
     7621 tcgacaatat gtatcatcac tccgaaaaag aaagctccca gtatagtttt aaaaatatgt
     7681 tttgtattta gaagagaact gataaaattt ggattttcac ttgttagggt atcaatgagt
     7741 ggaattataa aaaatatcac tgttccataa atcgaacctg ctttcagacc aggataacgt
     7801 aactgtctct tttctttttt catagggcac ctcctaatcc tcatcttgat ttttcttagt
     7861 ttttgcaatg cgacgggaga tgaggaactg tatgctcgct ccgaagaaaa tagaaccgag
     7921 aatgcttgat acaccatttc ttatagtgag aagagaatga aaatagtcct gaccttcacc
     7981 tatgagtatc ctgagaagag gagttataaa aaacatccat agaccaaaga acaaacctgc
     8041 tttcagacct gggtaatgta gttgcttact ttctttctcg ctcagcatgt ctggatcaat
     8101 agctgtaatc cctgtttttt catttggtag gtgacatagc cagcagcgat gagggcaatc
     8161 actaaaatca gaggaggata gattagagcc acttcttgag ggtatttata ggccagaagg
     8221 agtggaataa gatttccgaa aatcatcaga taaaagagga tgataaagac ttggttccca
     8281 atacgatcgg cctcacgccg tttgtattcg tcaaggggac cagaaatacc gtatgtgcgt
     8341 ttgatcagtt tttcagtgaa ggtttctttt ttcatgagtt tgctcctttt ttaaaaatct
     8401 tcctcccaaa agagactgtt gaggtcagtt tggaggctgc gggcgagatt gagacagagt
     8461 tccaaggttg gattgtactt gtcgttttca atcatattga tagtctgtct cgagacaccg
     8521 atatccttgg cgagttcgag ctgggaaata cccaattcct tgcgaaattc tttcacacga
     8581 ttcatctgtt ctcctttctg atttatgtcg tatatatttg actatattat agtcttttaa
     8641 acataaagtg tcaagtattt ttgacatatt ttttgaagaa atagtagtct ccttgtccta
     8701 tttgtctgac aagtgcaagc tggtcggatt tgtggtaaaa tagataagat atgacaaaag
     8761 aatttcatca tgtaacggtc ttactccacg aaacgattga tatgcttgac gtaaaacctg
     8821 acggtatcta cgttgatgcg actttgggtg gagcaggcca tagcgagtat ttattaagta
     8881 aattaagtga aaaaggccat ctctatgcct ttgaccagga tcagaatgcc attgacaatg
     8941 cgcaaaaacg cttggcacct tacattgaga agggaatggt gacctttatc aaggataact
     9001 tccgtcattt acaggcacgt ttgcgcgaag ctggtgttca ggaaattgat ggaatttgtt
     9061 atgacttggg agtgtctagt cctcaattgg accagcgtga gcgtggtttt tcttataaaa
     9121 aggatgcgcc actggacatg cggatgaatc aggatgctag tctgacagcc tatgaagtgg
     9181 ttaatcacta tgactatcat gatttgattc gtattttctt caaatacggt gaggataaat
     9241 tctctaaaca gattgcgcgt aagattgagc aagcgcgtga agtgaagccg attgagacaa
     9301 cgactgagtt agcagagatt atcaagttgg tcaaacctgc caaggaactc aagaagaagg
     9361 gtcatcctgc taagcagatt ttccaggcta ttcgaattga agtcaatgat gaactggggg
     9421 cggcagatga gtccatccag caggctatgg atatgttggc tctggatggt agaatttcag
     9481 tgattacctt tcattcctta gaagaccgct tgactaagca attgttcaag gaagcttcaa
     9541 cagttgaagt tccaaaaggc ttgcctttca tcccagatga tctcaagccc aagatggaat
     9601 tggtgtcccg taagccaatc ttgccaagtg cggaagagtt agaagccaat aaccgctcgc
     9661 actcagccaa gttgcgcgtg gccagaaaaa ttcacaagta agagggaaaa agatggcaga
     9721 aaaaatggaa aaaacaggtc aaatactaca gatgcaactt aaacggtttt cgcgtgtgga
     9781 aaaagctttt tacttttcca ttgctgtaac cactcttatt gtagccatta gtattatttt
     9841 tatgcagacc aagctcttgc aagtgcagaa tgatttgaca aaaatcaatg cgcagataga
     9901 ggaaaagaag accgaattgg acgatgccaa gcaagaggtc aatgaactat tacgtgcaga
     9961 acgtttgaaa gaaattgcca attcacacga tttgcaatta aacaatgaaa atattagaat
    10021 agcggagtaa gatatgaagt ggacaaaaag agtaatccgt tatgcgacca aaaatcggaa
    10081 atcgccggct gaaaacagac gcagagttgg aaaaagtctg agtttattat ctgtctttgt
    10141 ttttgccatt tttttagtca attttgcggt cattattggg acaggcactc gctttggaac
    10201 agatttagcg aaggaagcta agaaggttca tcaaaccacc cgtacagttc ctgccaaacg
    10261 tgggactatt tatgaccgaa atggagtccc gattgctgag gatgcaacct cctataatgt
    10321 ctatgcggtc attgatgaga actataagtc agcaacgggt aagattcttt acgtagaaaa
    10381 aacacaattt aacaaggttg cagaggtctt tcataagtat ctggacatgg aagaatccta
    10441 tgtaagagag caactctcgc aacctaatct caagcaagtt tcctttggag caaagggaaa
    10501 tgggattacc tatgccaata tgatgtctat caaaaaagaa ttggaagctg cagaggtcaa
    10561 ggggattgat tttacaacca gtcccaatcg tagttaccca aacggacaat ttgcttctag
    10621 ttttatcggc ctagctcagc tccatgaaaa tgaagatgga agcaagagct tgctgggaac
    10681 ctctggaatg gagagttcct tgaacagtat tcttgcaggg acagacggca ttattaccta
    10741 tgaaaaggat cgtctgggta atattgtacc cggaacagaa caagtttccc aacgaacgat
    10801 ggatggtaag gatgtttata caaccatttc cagccccctc cagtccttta tggaaaccca
    10861 gatggatgct tttcaagaga aggtaaaagg aaagtacatg acagcgactt tggtcagtgc
    10921 taaaacaggg gaaattctgg caacaacgca acgaccgacc tttgatgcag atacaaaaga
    10981 aggcattaca gaggactttg tttggcgtga tatcctttac caaagtaact atgagccagg
    11041 ttccactatg aaagtgatga tgttggctgc tgctattgat aataatacct ttccaggagg
    11101 agaagtcttt aatagtagtg agttaaaaat tgcagatgcc acgattcgag attgggacgt
    11161 taatgaagga ttgactggtg gcagaatgat gactttttct caaggttttg cacactcaag
    11221 taacgttggg atgaccctcc ttgagcaaaa gatgggagat gctacctggc ttgattatct
    11281 taatcgtttt aaatttggtg ttccgacccg tttcggtttg acggatgagt atgctggtca
    11341 gcttcctgcg gataatattg tcaacattgc gcaaagctca tttggacaag ggatttcagt
    11401 gacccagacg caaatgattc gtgcctttac agctattgct aatgacggtg tcatgctgga
    11461 gcctaaattt attagtgcca tttatgatcc aaatgatcaa actgctcgga aatctcaaaa
    11521 agaaattgtg ggaaatcctg tttctaaaga tgcagctagt ctaactcgga ctaacatggt
    11581 tttggtaggg acggatccgg tttatggaac catgtataac cacagcacag gcaagccaac
    11641 tgtaactgtt cctgggcaaa atgtagccct caagtctggt acggctcaga ttgctgacga
    11701 gaaaaatggt ggttatctag tcggggtaac cgactatatt ttctcggctg tatcgatgag
    11761 tccggctgaa aatcctgatt ttatcttgta tgtgacggtc caacaacctg aacattattc
    11821 aggtattcag ttgggagaat ttgccaatcc tatcttggag cgggcttcag ctatgaaaga
    11881 ctctctcaat cttcaagcaa cagctaaagc tttggagcaa gtaagtcaac aaagtcctta
    11941 tcctatgcct agtgtcaagg atatttcacc tggtgattta gcagaagaat tgcgtcgcaa
    12001 tcttgtacaa cccatcgttg tgggaacagg aacgaagatt aaaaacagtt ctgctgaaga
    12061 agggaagaat cttgccccga accagcaagt ccttatctta tctgataaag tagaggaagt
    12121 tccggatatg tatggttgga caaaggagac tgctgagacc cttgctaagt ggctcaatat
    12181 agaacttgaa tttcaaggtt cgggctctac tgtgcagaag caagatgttc gtgctaacac
    12241 agctatcaag gacattaaaa aaattacatt aactttagga gactaatatg tttatttccc
    12301 tcagtgctgg aattgtgaca tttttactaa ctttagtagg aattccggcc tttatccaat
    12361 tttatagaaa ggcgcaaatt acaggccagc agatgcatga ggatgtcaaa cagcatcagg
    12421 caaaagctgg gactcctaca atgggaggtt tggttttctt gattacttct gttttggttg
    12481 ctttcttttt cgccctattt agtagccaat tcagcaataa tgtgggaatg attttgttca
    12541 tcttggtctt gtatggcttg gtcggatttt tagatgactt tctcaaggtc tttcgtaaaa
    12601 tcaatgaggg gcttaatcct aagcaaaaat tagctcttca gcttctaggt ggagttatct
    12661 tctatctttt ctatgagcgc ggtggcgata tgctttctgt ctttggttat caagtgcatc
    12721 tagggatttt ctatattgtc ttcgctcttt tctggctagt cggtttttca aacgcagtaa
    12781 acttgacaga cggtgttgac ggtttagcta gtatttccgt tgtgattagt ttgtctgcct
    12841 atggagttat tgcctatgtg caaggtcaga tggatattct tctagtgatt cttgccatga
    12901 ttggtggttt gctcggtttc ttcatcttta accataagcc tgccaaggtc tttatgggtg
    12961 atgtgggaag tttggcccta ggtgggatgc tggcagctat ctctatggct ctccaccaag
    13021 aatggactct cttgattatc ggaattgtat atgtttttga aacaacttct gttatgatgc
    13081 aagtcagtta tttcaaactg acaggtggta aacgtatttt ccgtatgacg cctgtacatc
    13141 accattttga gcttggggga ttgtctggta aaggaaatcc ttggagcgag tggaaggttg
    13201 acttcttctt ttggggagtg ggacttctag caagtctcct gaccctcgca attttatatt
    13261 tgatgtaaga atggcaccct gatgtttcag ggtgtttttg tgtttaaata cacaatgaaa
    13321 atcaaagaac aaactagaaa gctaacttta ggctgctcaa aatatagtag attgaaacta
    13381 gaatagtaca catctacttc taaaatattg ttagaaatcg atttgactgt cctgatcgat
    13441 ttgtcctgtt cttatttcat tttaatatac agttttgagg ttgtagataa ggagaagctg
    13501 atgtggtttg aagaggtttt cgaagagtgt aaaaaaatat tggtcagtta aagtcaaaac
    13561 tcttgacctt ttctgactaa tgtagtatat tatgaacgta aggtaaatga aagccttact
    13621 agaacactta tttaaagtaa aatagaaaga ggtgggtcta tgtttcatct agaaatcttc
    13681 agaagtaaag atagtctact cctgcttgaa aaagaaaaac cagaaatagt acgtagggta
    13741 gcgatctagc tagtctaaat tttttaaaac aatggtcaaa gatagtcaat atcagtaatc
    13801 ataactaagt aaacaaaaag aggtaaagaa tatgaataac aactttaata attttaataa
    13861 catggatgat ttatttaacc aattgatggg tggtatgcga ggatacagtt ctgaaaatcg
    13921 ccgttactta attaatggac gcgaagtcac acctgaggaa tttgctcact atcgtacgac
    13981 tggtcaatta ccaggaaatg cagaaactga tgtgcaaatg ccacaacagg catcaggtat
    14041 gaaacaagac ggtgtccttg caaaactagg tcgaaacttg acagcagaag cgcgtgaggg
    14101 caagttggat cctgttatcg gacgaaacaa ggaaattcaa gaaacatctg aaatcctctc
    14161 acgccgcacc aagaacaatc ctgttttggt cggagatgca ggtgttggta agacagcagt
    14221 tgtcgaaggt ctagcgcaag ccattgtgaa cggagatgtt cctgctgcta tcaagaacaa
    14281 ggaaattatt tctattgata tctcaggtct tgaggctggt actcaatacc gtggtagctt
    14341 tgaagaaaat gtccaaaact tagtcaatga agtgaaagaa gcagggaata ttatcctctt
    14401 ctttgatgaa attcaccaaa ttcttggtgc tggtagcact ggtggagaca gtggttctaa
    14461 agggcttgcg gatattctca agccagctct ctctcgtgga gaattgacag tgattggggc
    14521 aacaactcaa gacgaatacc gtaacaccat cttgaagaat gctgctcttg ctcgtcgttt
    14581 caacgaagtg aaggtcaatg ctccttcagc agagaatact tttaaaattc ttcaaggcat
    14641 tcgtgacctc tatcaacaac accacaatgt catcttgcca gacgaagtct tgaaagcagc
    14701 ggtggattat tctgttcaat acattcctca acgtagcttg ccagataagg ctattgacct
    14761 tgtcgatgta acggctgctc acttggcggc tcaacatcca gtaacagatg tgcatgctgt
    14821 tgaacgagaa atcgaaacgg aaaaagacaa gcaagaaaaa gcagttgaag cagaagattt
    14881 tgaagcagct ctaaactata aaacacgcat tgcagaattg gaaaagaaaa tcgaaaacca
    14941 cacagaagat atgaaagtga ctgcaagtgt caacgatgtg gctgaatctg tggaacgaat
    15001 gacaggtatc ccagtatcgc aaatgggagc ttcagatatc gaacgtttga aagatatggc
    15061 tcatcgcttg caagacaagg tgattggtca agataaggcc gtagaagctg tagctcgtgc
    15121 tatccgtcgt aaccgtgctg gttttgatga aggaaatcgc ccaatcggca gcttcctctt
    15181 tgtagggtct actggggttg gtaagacgga gcttgctaag caattggcac tcgatatgtt
    15241 tggaacccag gatgcgatta tccgtttgga tatgtctgaa tacagtgacc gcacagctgt
    15301 ttctaagcta attggtacaa cagcaggcta tgtgggttat gatgacaata gcaatacctt
    15361 aacagaacgt gttcgtcgca atccatactc tatcattctc ttggatgaaa ttgaaaaggc
    15421 tgatcctcaa gttattaccc ttctcctcca agttctagat gatggtcgtt tgacagatgg
    15481 tcaaggaaat acagtaaact tcaagaacac tgtcattatt gcgacctcaa atgctggatt
    15541 tggctatgaa gccaacttga cagaagatgc ggataaacca gaattgatgg accgtttgaa
    15601 acccttcttc cgtccagaat tcctcaaccg ctttaatgca gtcatcgagt tttcacactt
    15661 gactaaggaa gacctttcta agattgtaga tttgatgttg gctgaagtta accaaacctt
    15721 ggctaagaaa gacattgact tggtagtcag tcaagcggct aaagattata tcacagaaga
    15781 aggttacgac gaagtcatgg gggttcgtcc tctccgtcgc gtggttgaac aagaaattcg
    15841 tgataaggtg acagacttcc acttggatca tttagatgct aaacatctgg aagcagatat
    15901 ggaagatggc gttttggtta ttcgtgagaa agcctaagac agaattttga gaataaaaaa
    15961 gaaggagcca gctgaaaaaa actggttcct tttgaggtac gacgggcatg tcgtgtagta
    16021 gagatgtact attctagttt caatctacta tagtagctca gaaatcggta cttaaacgtg
    16081 ctatatcaaa accagtcctt gaaaaacgtg gactggtttc gtgtttggat tattactttg
    16141 aacgacatgc gttaaaagtt agttgaaccg ccgtatgccg aacggtacgt acggtggtgt
    16201 gagaggggct agagattatc ccctactcga ttttaaatca catgacgttc aaaggcatca
    16261 tctgaaatcc cttgttccaa gatgagtttt gcccattctt tagcagagaa gaggctgtgg
    16321 tccttgtagt ttccgcaaga ttcgatggtt gtccctggga catcttccca agtagtagtt
    16381 tcagcgattt ccttgagcga atccttgata acagctgcga ttttagcact ggtgtgacgt
    16441 ccccacataa tcatgtggaa gcctgtgcgg caaccaaatg gcgaacagtc aatcatgccg
    16501 tcaatgcggg tacggatgag tttggctaag aggtgctcga tagtgtgaag gccggcggta
    16561 gggatagagt cttcgtttgg ttgcaccaag cgaatatcat aattggagat gatgtctcct
    16621 tttggtcctg tttcttcccc aatcaagcga acatagggtg ctttgacaat ggtgtggtca
    16681 agttcaaaac tttcgacaat aacttctttt gacatggtaa atcctttcag ttttcttctc
    16741 tcattatatc ataaaggttg ctcctgagac agagagaaaa cctctccgag gctggagagg
    16801 ttgaaatctt tacttacgat ataagcggtc gtattggtag tatgggtcaa aggttacgtt
    16861 gatacccagt ttacgaagga cattcttgtc ttcatcagtc aagatgatgg ttgagtgggc
    16921 ttcgcttcct ttgaggttgc cgagttcttc catagcgcgg gtagcatcag gattttctgt
    16981 agctgtgata gcaagtgcaa tcaggatttc atttgaatga aggcgtggat tgcggctacc
    17041 gagatgatcg attttaagac cttggattgg cttaacaact tccggctcga ttaattttac
    17101 ttctttagcg atgtcagctg attttttgat ggcgttgatc aaggcagcgg ctgtagggcc
    17161 aaagagttct gagttcttac cagtgacgat ttccccattt ggcaattcaa aggctagggc
    17221 tggtccacca gtttcttctg ctttttggcg cgcaacgaca gcaaccttac ggtctgcagg
    17281 tgtgataccg aggtcgttca tgagcaactc aattttcttg acggcagctt cgccaacttt
    17341 ttcagctttg aagtcaagaa cggtttgata gtaacggcgg atgatttctt gtttagaagc
    17401 ttcgacagcg gcctcgtcat ctgtaatagc gaaaccaacc atgttgacac ccatatctgt
    17461 cggtgaagcg tatggtgatt ttccgagaat acgttccaac atgcgtttga gcactgggaa
    17521 gatttcgata tcacggttgt agttgacagt ggtttctcca taggtttgga gatggaaggg
    17581 gtcaatcatg ttgacatcat caaggtcagc tgtggcagct tcataagcca agttaactgg
    17641 atgatgaagg ggaagattcc aaacagggaa ggtttcaaat ttagcgtagc cagatttgat
    17701 gccattgatt tggtcgtggt acatattgga catacacgtt gccaattttc cagaaccagg
    17761 tccaggagcg gttacgacaa tcaagttgcg actggttttg atgtagtcgt ttttgcccat
    17821 gccttctggg gaaatgatgt gatccatatc cgtcggatat cctttgattg gataatgaag
    17881 atatgaatca attccgtttt tctcaagttg gttgcggaag gcatctgcag cgggttggcc
    17941 agcgtattgt gtaatgacaa cggaaccaac aaaaatcccc aattcattga atttgtcaat
    18001 caaacgaaga acttcttggt cataagaaat gcctaagtcg ccacgtgctt tggaatgttc
    18061 aatgttgcta gcattaatgg caatcacaac ctcaacctgc tctttcaatt cttgcaagag
    18121 cttgattttg ttgtcaggtt cataaccagg aaggacacga gcagcgtgga aatcttctaa
    18181 cattttaccg ccaaactcta agtagagctt gccgtcaaat tggttaatac gctccaaaat
    18241 atggtcgcgt tgtaaattca aatattgttc agaactaaaa gcttgttttt tcattttttt
    18301 acctctggac tctattataa taaaaaattg gaagttagga aactacggag ctaaaaaaga
    18361 aattaaaaag attaagcaaa cgcttgcaca aaattttaaa aagtgctatc atagactata
    18421 gattattaaa ataatgaggt aaacagatgc aagaaaaatg gtggcataat gccgtagtct
    18481 atcaagtcta tccaaagagt tttatggata gtaatggaga tggagttggt gatttgccag
    18541 gtattaccag taagttggac tatctagcca agctaggaat cacagcaatt tggctttctc
    18601 ccgtttatga cagccctatg gatgataatg gttacgatat tgctgattat caagcgattg
    18661 caacgatttt tggaaccatg gaggatatgg atcaactgat tgcagaagct aagaagcgtg
    18721 atatccgtat catcatggac ttggtggtca atcatacctc ggatgagcat gcctggtttg
    18781 tagaggcctg tgaaaatcct aatagccctg agcgagacta ctatatctgg cgcgatgaac
    18841 ccaatgacct agattctatc tttagtgggt ctgcttggga atacgatgaa aagtcaggtc
    18901 aatactatct ccactttttc agcaagaaac agccggatct caactgggaa aatgaaaaac
    18961 ttcgccagaa aatttatgag atgatgaact tctggattga taaaggtatt ggtggtttcc
    19021 gtatggatgt tattgacatg attggcaaaa ttcctgacga gaaggtagtc aataatggtc
    19081 ctatgctcca tccctatctc aaggaaatga atcaggcgac ctttggagat aaggatctct
    19141 tgacagtagg ggagacttgg ggagcaacgc cagagattgc caagctctac tctgatccaa
    19201 aggggcaaga attgtctatg gtcttccagt ttgaacatat cggtcttcag tatcaggaag
    19261 gtcagcctaa atggcactat caaaaagagc tgaatatcgc taagttaaaa gaaatcttca
    19321 acaaatggca gacagagtta ggagttgagg acggctggaa ttccctcttc tggaacaacc
    19381 atgacctccc tcgtattgtc tcaatatggg gaaatgacca agaataccgc gaaaaatctg
    19441 ccaaagcctt tgcaatcttg cttcatctta tgagaggaac tccttatatc taccaaggtg
    19501 aggagattgg gatgaccaac tatccgtttg aaacactaga tcaagtagaa gatattgaat
    19561 ctctcaacta tgcgcgtgag gctcttgaaa aaggtgttcc gattgaagaa atcatggaca
    19621 gtatccgtgt tattggacgt gacaatgccc gtacccctat gcaatgggac gagagcaaaa
    19681 acgctggttt ctcaacaggt caaccttggt tggcggttaa tccaaattac gagatgatca
    19741 acgtccaaga agcgctggca aatccagatt ctattttcta tacctatcag aaactggtcc
    19801 aaattcgcaa ggagaatagc tggctagttc gagctgactt tgaattgctt gatacggctg
    19861 ataaggtctt tgcttatata cgtaaggatg gcgaccgtcg cttcctagtt gtggctaact
    19921 tgtccaatga agagcaagac ttgacagtag aaggaaaagt caaatctgtc ttgattgaaa
    19981 acaccctagc tcaagaagtc tttgaaaaac aaatcttagt tccatgggat gctttctgtg
    20041 tggaaatgac tgactagaat gagcaaacct caagtttttg aagcttgagg ttttttacta
    20101 tagtggattg aaactagaat agtgcacctc tgcttctaaa acattgttag aaatcgattt
    20161 gactgtcccg atcgatttgt cctattctta tttcattttg ctatacttgt agttgaggag
    20221 aattaagctc ctcactagta aactcttcat aatccttttt atttttatga agatattgtt
    20281 tgaaagatgt gagtttccac ggatgggttt gtggagggat atacttgcgt ctttcttttt
    20341 ttgttctggt tcttgttcaa agtttttcga atagagttca tgatttagta gctcctttgt
    20401 gtgatagatt ttgtcagcga tattgaggta gatgtctccg tcaaatgctt ttataactaa
    20461 tgctttcgtc tttctgatga aatagacttc ttttccttgt tcggtaggga tatagtaacg
    20521 attttggaat cggatatggt gtccactatc gacgactctc tccgccagtc tagccagaat
    20581 gagattcctt tcagaggggt taggaacctc ctcgaagaca gagagttttg tcttgtttcc
    20641 aaactgttca ttaaaggttt gaatgtagga aagcaggaag gtattggctt cttccaaggt
    20701 atgaatattg ttttgttcca gttcgatagg caggcgagat tgtagtgtct gattgagtct
    20761 ttcgactctc cctttagctt gagggataga ggttgtctca aggagaatcc ctagttggtg
    20821 acaggcgtat ccaaactgtg tatgggtatc gtcctccatt ttcttagagt tggaggcttg
    20881 ataggtaaag accgttctct tatctgtttt aatttgaagg ggaatgccgt gattggctaa
    20941 gatttgttcg aggacatgat agtaagcatt caaggtctct tgtttatcaa aataagcgcc
    21001 taggatattg ccagaagcat catcaatggc taggtgtaag ttagaggttt ctgctccaaa
    21061 ccaggcatga gggctggtat ccatttgaat gagttcacca gcaaatttct ttctgggtct
    21121 actaggatgt acttttttag ggttttccag gaagtcttca gccgtcggta agattagatt
    21181 gtctaggggt tgcttggggt tcagtttagc ttgttttctt actctcttct ttgtctttct
    21241 atgggactta ggcgacagga tatttttctt atagagtatt tttctaacag ttgtatcaga
    21301 gagctgaatt ccttcttctt cagctagcaa ttcacagaaa tgacggacat ttggtttata
    21361 tgtttcatag gagaggtatt tctttaggat acgttctttg atttcatcag ggattgcatg
    21421 ttttggtttt cgatttctgt ttccgtgtct gaaggcttct tttcctttct gttgataggc
    21481 tagtagcaga cgattgattt gcctttcaga aagattgagc acgacacagg ctcgtttctt
    21541 tgttttcttt ccttgggcta tagcttttat cacaagatat tttttcgttt cattcatatt
    21601 cagttggatc ctttacatat gactattcta ccaaatggga catttttacg ttcgatttac
    21661 taaagacatt atcacattcg aattacacaa gatgcagata gtgaaaaaag gtgtagacat
    21721 taccgtaaaa aagtgatata atcgtatgat gttcaatgta taggtgttaa tcatgagtag
    21781 acgttttaaa aaatcaggtt cacagaaagt gaagcgaagt gttaatatag ttttgttgac
    21841 tatttattta ttgttagttt gttttttatt gttcttaatc tttaagtaca atatccttgc
    21901 ttttagatat cttaatctag tggtaactgc gttagtccta ctagttgcct tggtagggct
    21961 actcttgatt atctataaaa aagctgaaaa atttactatt tttctgttgg tgttctctat
    22021 ccttgtcagc tctgtgtcgc tctttgcagt acagcagttt gttggactga ccaatcgttt
    22081 aaatgcgact tctaattact cagaatattc aatcagtgtc gctgttttag cagatagtga
    22141 gatcgaaaat gttacgcaac tgacgagtgt gacagcaccg actgggactg ataatgaaaa
    22201 tattcaaaaa ctactagctg atatcaagtc aagtcagaat accgatttga cggtcaacca
    22261 gagttcgtct tacttggcag cttacaagag tttgattgca ggggagacta aggccattgt
    22321 cctaaatagt gtctttgaaa acatcatcga gtcagagtat ccagactacg catcgaagat
    22381 aaagaagatt tataccaagg gattcactaa aaaagtagaa gctcctaaga cgtctaagaa
    22441 tcagtctttc aatatctatg ttagtggaat tgacacctat ggtcctatta gttcggtgtc
    22501 gcgatcagat gtcaatatcc tgatgactgt caatcgagat accaagaaaa tcctcttgac
    22561 cacaacacca cgtgatgcct atgtaccaat cgcagatggt ggaaataatc aaaaagataa
    22621 attaacccat gcgggcattt atggagttga ttcgtccatt cacaccttag aaaatctcta
    22681 tggagtggat atcaattact atgtgcgatt gaacttcact tctttcttga aaatgattga
    22741 cttattggga gggatagatg ttcataatga tcaagagttt tcagctctac atgggaagtt
    22801 ccatttccca gtagggaatg tccatctaga ctctgagcaa gctttaggtt ttgttcgtga
    22861 gcgctactcc ctagcagatg gcgatcgtga ccgtggtcgc aaccaacaaa aggtgattgt
    22921 ggctatcctt caaaaattaa cgtcaaccga agcgctgaaa aattatagta cgatcattga
    22981 tagcttgcaa gattctatcc aaacaaatat gccacttgag accatgataa atttggtcaa
    23041 tgctcagtta gaaagtggag ggaattataa agtaaattct caagatttaa aagggacagg
    23101 tcggacggat cttccttcct atgcgatgcc agatagtaac ctctatgtga tggaaattaa
    23161 cgacagtagc cttgcatctg tcaaaactgc tattcaggac gtgttggagg gcagatgaaa
    23221 tgattgatat tcattcgcac attgtctttg atgtagatga tggtcccaag tcaagagagg
    23281 aaagcaaggc tctcttgaca gaagcctaca ggcagggggt gcgaaccatt gtctctacct
    23341 ctcaccgtcg caagggcatg tttgaaactc cagaagagaa gatagcagaa aactttcttc
    23401 aggttcggga aatagctaag gaagtcgcga gtgacttagt cattgcttat ggggctgaaa
    23461 tttactacac accagatgtt ttggataagc tggaaaaaaa tcggattccg accctcaata
    23521 atagtcgtta tgccttgata gagtttagta tgaacacccc ttatcgcgat attcatagtg
    23581 ccttgattaa aatattgatg ttgggaatta ctccagtcat tgcccacatc gagcgctatg
    23641 atgcacttga aaacaatgaa aaacgcgttc gagagctgat cgatatgggc tgttacacgc
    23701 aagtaaatag ttcacatgtc ctcaaatcca aactttttgg agaacggtat aaattcatga
    23761 aaaaaagagc gcagtatttc ttggagcgtg atttggttca tgtgattgca agtgatatgc
    23821 ataatgtgga cagcagacct ccccatatgg cagaagcata tgaccttgtt tctcaaaaat
    23881 acggagaagc gaaggctcag gaacttttta tagataatcc tcgaaaaatt gtaatggatc
    23941 aactaattta ggagaaataa tgaaagaaca aaatacgata gaaatcgatg tatttcaatc
    24001 acttaaaacc ttatggaaat acaagctaat cattttatta gtggcacttg tgacaggggc
    24061 gggagctttt gcatatagca cttttattgt taagccagaa tatacaagta ccacgcgtat
    24121 ttacgtagtc aaccgtaatc aaggagataa gccgggactg acgaatcagg acttgcaggc
    24181 aggaacttat ctggtaaaag actaccgcga aattatcctt tcgcaagatg tattggaaaa
    24241 ggtagcgaca aatttgaaat tggatattcc agcaaaaacg ttaactagca aagtgcaagt
    24301 gactgtacca gccgacactc gtatcgtctc aatctctgtc aaggataaac aaccagagga
    24361 agccagtcgc attgctaatt ctctacgaga agttgctgca gaaaagatca tcgctgtaac
    24421 gcgagtatct gatgtaacga cgcttgaaga agcgcgacca gctacgactc cctcttctcc
    24481 aaatgttcga cgcaacacct tagttggttt tcttggagga gccgccgtaa cagtaattac
    24541 tgttcttttg attgagttgc tcgatacccg tgtaaaacgt cctgaagatg ttgaagatgt
    24601 actgcaaatg ccacttctag gagtcgttcc agattttgat aaaatgaaat aggaggaagt
    24661 tatgccaacg ttagaaatct cacaggtgaa attagaactt gcgaaaaagg cagaggaata
    24721 ttataatgct ttgtgcacga acctacagtt aagtggagat gatttgaaag tattttctat
    24781 cacttctgtg aaagcaggag aaggaaaaac aacgacttcc accaatatcg cttgggcttt
    24841 tgcgcgtgca ggttacaaaa cgctgctgat tgatgcagac atgcgtaact ccgttatgtc
    24901 aggtgtcttt aaatcaaggg aaaggattac tggactaaca gaatttctat cagggacaac
    24961 agacctgtca caggggcttt gcgataccaa tattgagaat ctctttgtga ttcaggctgg
    25021 ctctgtgtca ccaaatccga cagcccttct tcaaagtaag aatttcagca caatgcttgg
    25081 aaccttgcgt aaatattttg actatatcgt cgtagacact gctcctatcg gtattgtgat
    25141 tgatgcggct atcattatgc agaaatgtga tgcttctatt ttagtgacga atgcaggtga
    25201 aacaaaacga cgggatcttc aaaaagcgaa agaacagttg gaacaaactg ggaagtcgtg
    25261 tttaggagtt gtgttgaata aattcgatac ttcagtagag aaatacggtt cttatgggag
    25321 ttatgggaat tacaggaaac agaaaaaata ggttgaggga tagaaatgag tgaaaaacta
    25381 gcaaagcctt cagtagccat agtccagagt tttcttgtta ttttattgac ttatctactt
    25441 agcgctgtga gagaaacaga gattgtttca acaacagcta ttgtacttta tatcctccac
    25501 tattttgtct tttatatcag tgattatgga cggaatttct ttaaaagggg atatttgatt
    25561 gaacttgtcc agacattgaa atatatccta ttctttgcgc tagcgattag tatttctaat
    25621 tttttcttag aagatcgatt tagtatttcc agaagaggca tgatttactt cctcttatta
    25681 catgttttct tagtctatgt gctaaaccta tttatcaagt ggtattggaa gcgggcttat
    25741 cccaacttta aaggaagtaa gaaggttttc ctacttacag caacttctca tgtcgaaaag
    25801 gtactggata gattaataga atcaaatgag gttgttgggg agttggtagc cgtcagtgtt
    25861 ttagataaac cagattttca gcatgattat ttaaaggttg tagcagaggg ggagatagta
    25921 aactttgcga ctcatgaggt ggtcgatgaa gtctttatca atcttccaag tgaaaaatac
    25981 aatattggag agcttgtctc tcagtttgaa acgatgggaa ttgatgtaac agtcaatcta
    26041 aatgctttcg attgggcacg taacaagcaa attcgtgaga tggcaggact aaatgttgtg
    26101 actttttcta caacatttta taagactagc catgtgattg ctaagtggat tattgatatt
    26161 gtcggttccc tggtaggttt gatattatgt ggtctagtca gtattgtgct ggttcctttg
    26221 attcgaaagg atgggggttc tgcttttttt gctcagacgc gtataggaaa aaatggccgc
    26281 catttcactt tttataagtt tcgctctatg tgtgtagatg ctgaggataa aaagagagaa
    26341 ctcatggaac aaaataccat gcagggtgga atgtttaagg tggacgatga tcctcgtatc
    26401 acgaaaattg gtcattttat acggaagacg agcttggacg agctaccaca gttttataat
    26461 gttctaaagg gagatatgag tttggtaggt acacgaccac caacagtgga cgagtatgaa
    26521 cactataccc cagaacaaaa acgtcggcta agttttaaac ctggtataac aggcttatgg
    26581 caggtcagtg gacgaagtga gatcaagaat ttcgatgaag ttgtcaagtt agatgtggcc
    26641 tatatagacg attggacaat ctggaaagat attgagattt tattgaagac agttaaagtt
    26701 gtattgatga aggatggagc gaaataggaa atgaaaatat gtttagttgg gtctagtgga
    26761 ggtcatctga ctcatttata tctattaaaa cctttctgga aagataagga aagattttgg
    26821 gttacttttg ataaagagga tacaagaagt atcttgggaa atgagacatt ttatccttgt
    26881 cattatccta ctaatcgtaa tttgaaaaat cttattaaaa atacggttct tgctttcaat
    26941 attttgagaa aggaacgccc tgatgttatc atctcatcag gggctgctgt agcagttcct
    27001 ttcttttacc taggaaaact atttggcgct aagacagtct acattgaagt ttttgatcgt
    27061 attgatgcgc caaccttaac aggaaaaata gtatatccag taacagataa gttcatagtt
    27121 cagtgggagg aaatgaaaaa agtatatcca aaagcaatta atctcggagg gattttctaa
    27181 tgatttttgt gacaataggt actcatgagc aacagtttaa tcgacttatt aaagaagttg
    27241 atagattaaa aggtgaggga tttattcagg atgatgtttt tattcagact ggtttttcag
    27301 attatgaacc agtgtattgt caatggaaga atctgatttc ttatgatgag atgaatcgct
    27361 atatagatga agcaaatatt atcattacac atggcggccc agctacattt atggcagtta
    27421 ttgctaaagg aaaaatacca atagttgttc cgagattaaa gaagtttggt gagcatgtga
    27481 atgatcatca gatggaattt tgttctaaaa tactatccga agggtatgaa ttatctctga
    27541 ttaatgatat aagcgaattg cagtatagtt taaagcaaga aaagaaaact aaattttact
    27601 ctaataatac atgttttctt aagaaattat caaaaaca
//
LOCUS       DAVKJJ010000027        27341 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000027 DAVKJJ010000000
VERSION     DAVKJJ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27341
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.027"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(<1..399)
                     /locus_tag="VW144_001182"
     CDS             complement(<1..399)
                     /locus_tag="VW144_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600634.1"
                     /translation="MYLDGGAYLSKSACQGICAALAMSPGTFIALTGAAVLTKKLINY
                     IKVGGLGGWLIGAAAGVLALIFLIKICVDTTIKVLLNRIFKASKVMKRRIFSLFVLDL
                     MVYIFLGYVLVIQKDVYLFSILIIFSNFSVP"
     gene            complement(428..766)
                     /locus_tag="VW144_001183"
     CDS             complement(428..766)
                     /locus_tag="VW144_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEU9600635.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     EKINGTRITK"
     gene            1232..2095
                     /locus_tag="VW144_001184"
     CDS             1232..2095
                     /locus_tag="VW144_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9600636.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYIRQHGAGELSDHAEQVVKKLWRKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKK
                     EVETHYQPKKL"
     gene            complement(2282..3673)
                     /gene="argH"
                     /locus_tag="VW144_001185"
     CDS             complement(2282..3673)
                     /gene="argH"
                     /locus_tag="VW144_001185"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU9600637.1"
                     /translation="MAKNTKLWGGRFEGTVEDWVERFGASISFDQKLAKFDLIGSLAH
                     VQMLGQTGILSLEESEKIQAGLKELLEELEAGQLDFDIANEDIHMNMEVLLTEKIGPL
                     AGKLHTARSRNDQVATDMHLYLKEQLGHVLDKLVHLKGVLLDLAENHVETIMPGYTHL
                     QHAQPISFAHHLMAYYNMFQRDSERFEFNQKHTDLCPLGAAALAGTTFPIDRQLSSDL
                     LEFKKPYTNSLDAVSDRDFILEFLSNASILMMHMSRFCEEMINWCSFEYQFITLSDTF
                     TTGSSIMPQKKNPDMAELIRGKTGRVYGHLFGLLTVMKSLPLAYNKDLQEDKEGMFDT
                     VETILNSLDVLAGMLSSLQVNKEKMQESTEKDFSNATELADYLAGKGLPFREAHEVVG
                     RLVLDSIKSAKNLQDWTLEELQTYHSLITEDIYVYLQPKTAVQRRNSLGGTGFDQVEY
                     QIAVAKKANEAKK"
     gene            complement(3702..4898)
                     /locus_tag="VW144_001186"
     CDS             complement(3702..4898)
                     /locus_tag="VW144_001186"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU9600638.1"
                     /translation="MSKEKVILAYSGGLDTSVAITWLKKDYDVVAVCMDVGEGKDLDF
                     IHDKALKVGAVESYVIDVKDEFATDYVLVAHQSHAYYEQKYPLVSALSRPLISKKLVE
                     IAHQIGATTIAHGCTGKGNDQVRFEVSIAALDLNLKVIAPVREWKWSREEEIYYAKEN
                     GVPVPADLDNPYSVDQNLWGRANECGILENPWNQAPEEAFGITSSPEAAPDMPEYIDI
                     EFSEGVPVSLNGEVLKLADLIQKLNEIAGKHGVGRIDHVENRLVGIKSREIYECPGAV
                     TLLTAHKEIEDLTLVREVAHFKPIIENELSNLIYNALWFSPATQALIAYIKETQKVVN
                     GTAKVKLYKGSAQVVARKSPNSLYDENLATYTSADTFDQDAAVGFIKLWGLPTKVHSE
                     VQKSAK"
     gene            complement(4915..5721)
                     /locus_tag="VW144_001187"
     CDS             complement(4915..5721)
                     /locus_tag="VW144_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9600639.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSTPYYTAKNKLIVKKSDLATYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVAKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(5928..6569)
                     /locus_tag="VW144_001188"
     CDS             complement(5928..6569)
                     /locus_tag="VW144_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9600640.1"
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRVIELGEGK"
     gene            complement(6566..8674)
                     /locus_tag="VW144_001189"
     CDS             complement(6566..8674)
                     /locus_tag="VW144_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9600641.1"
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGTLVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTAL
                     LIGVGRSLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPNTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     GLASSMEKRSLVELLKGGIL"
     gene            complement(8726..9013)
                     /locus_tag="VW144_001190"
     CDS             complement(8726..9013)
                     /locus_tag="VW144_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU9600642.1"
                     /translation="MRKLVKIGVASLMVCGILATTNALAVWVEGGQWNYGVGWTGTFG
                     YSDYLHSTRYHTATVRHGGRTSKDYAKPEAWARASLTKIPPTGMEYFYGFE"
     gene            9444..9764
                     /locus_tag="VW144_001191"
     CDS             9444..9764
                     /locus_tag="VW144_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9600643.1"
                     /translation="MQRLEVYKNYQRLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     gene            complement(9816..10403)
                     /locus_tag="VW144_001192"
     CDS             complement(9816..10403)
                     /locus_tag="VW144_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9600644.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            10645..11316
                     /gene="sdaAB"
                     /locus_tag="VW144_001193"
     CDS             10645..11316
                     /gene="sdaAB"
                     /locus_tag="VW144_001193"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEU9600645.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            11325..12197
                     /gene="sdaAA"
                     /locus_tag="VW144_001194"
     CDS             11325..12197
                     /gene="sdaAA"
                     /locus_tag="VW144_001194"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEU9600646.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            12219..12851
                     /locus_tag="VW144_001195"
     CDS             12219..12851
                     /locus_tag="VW144_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9600647.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(13111..14961)
                     /locus_tag="VW144_001196"
     CDS             complement(13111..14961)
                     /locus_tag="VW144_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9600648.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVARAVDEAKVPKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(15000..16160)
                     /locus_tag="VW144_001197"
     CDS             complement(15000..16160)
                     /locus_tag="VW144_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9600649.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKGTIIYLDKISQINQNMLQ"
     gene            16218..17384
                     /locus_tag="VW144_001198"
     CDS             16218..17384
                     /locus_tag="VW144_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEU9600650.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
     gene            17523..17849
                     /locus_tag="VW144_001199"
     CDS             17523..17849
                     /locus_tag="VW144_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9600651.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            17836..18429
                     /locus_tag="VW144_001200"
     CDS             17836..18429
                     /locus_tag="VW144_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEU9600652.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTILAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            18422..19333
                     /locus_tag="VW144_001201"
     CDS             18422..19333
                     /locus_tag="VW144_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEU9600653.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHALTITDSFDDQIHISYHPSLSANHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILQLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            19396..20460
                     /locus_tag="VW144_001202"
     CDS             19396..20460
                     /locus_tag="VW144_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600654.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEERAQEVADKAEETIANLDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIEAPEDSEARTGIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIVSILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(20541..21392)
                     /locus_tag="VW144_001203"
     CDS             complement(20541..21392)
                     /locus_tag="VW144_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEU9600655.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDVPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEIQPILV
                     QDFKTLA"
     gene            complement(21514..22500)
                     /locus_tag="VW144_001204"
     CDS             complement(21514..22500)
                     /locus_tag="VW144_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEU9600656.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEESEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(22772..24247)
                     /locus_tag="VW144_001205"
     CDS             complement(22772..24247)
                     /locus_tag="VW144_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEU9600657.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKSVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(24351..25274)
                     /locus_tag="VW144_001206"
     CDS             complement(24351..25274)
                     /locus_tag="VW144_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9600658.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(25288..26217)
                     /locus_tag="VW144_001207"
     CDS             complement(25288..26217)
                     /locus_tag="VW144_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9600659.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(26536..26757)
                     /locus_tag="VW144_001208"
     CDS             complement(26536..26757)
                     /locus_tag="VW144_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600660.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQEELQKAVLF
                     LADEKGSEHTAAELIDNLKEVIAKLKANA"
     gene            complement(26711..26971)
                     /locus_tag="VW144_001209"
     CDS             complement(26711..26971)
                     /locus_tag="VW144_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600661.1"
                     /translation="MTGVSNPTTDSARLVLADSKKAFADDSLIEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
     gene            27051..>27341
                     /locus_tag="VW144_001210"
     CDS             27051..>27341
                     /locus_tag="VW144_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9600662.1"
                     /translation="MKSTKEEIQTIKTLLKDSRTAKYHKRLQIVLFRLMGKSYKEIIE
                     LLDCNQTTIWRNVKKYEEFGLDSLLQETRGGRNHAYMTVEEEKVFLARHLKAA"
BASE COUNT         8265 a         5950 c         4863 g         8263 t
ORIGIN      
        1 gggaactgaa aaattagaaa aaattatcag aattgaaaac agatatacgt ctttttgtat
       61 cactaaaaca taacctaaaa atatatagac catcaaatct agtacaaaaa gagaaaatat
      121 cctccttttc attaccttac ttgccttaaa gattctgttt aatagaacct ttatcgtagt
      181 atctacacaa atttttatca aaaagataag agccaataca cctgctgctg caccaataag
      241 ccaacctcca aggcctccaa ccttaatata gtttattagt ttttttgtta aaactgcagc
      301 tccagtcaat gctataaaag ttcctgggct catagctaaa gctgcgcaaa ttccttgaca
      361 agcactcttg cttaaataag cccccccatc aagatacatc atctcctctt gctcaagagc
      421 aacataatta tttggtaata cgagttccat ttattttctc ctttatacta tgtaatacca
      481 caaaatgatt ttgacctaaa tcattcttaa cttgaagaat cgcaggaaat gttaattttg
      541 gagtcaaatc ttccagtgcc actcgaacag cttttactgt aaaattcaac ttattcagac
      601 ctgaaacaat accactgaca gtcgttccat acatgtctgt accaataatt tcccgaatct
      661 tcatgattga tagttccttt ttgtaagata aaagaactgt tgaaacaact gccgcagcac
      721 aatctgactg atcatgttgc atcgtacaag gatatttttt taacatagaa atactcaatt
      781 ctttagaatt aatctaactt ttctttgatg tcacccaaac ctcaatcact gcccagtagt
      841 tcttgaatca gtttattttc aatcgtatca ttctttgttc ttgatatctt tccaagcatt
      901 gtagtacagc agatttttgt ccaaggactg agcctccttt aacaactcaa gctcccttac
      961 attatatcga ccagccccat ggaaagatta tgaccagact aatcaatgat atcatacata
     1021 ctagaataca aaatattttt ttattcatac tttttctcct atctttattt tctacctaca
     1081 tcatacctct tattaagctc attttaaaat tacgttaact tttttcacaa atttatttcg
     1141 actttctttt ttacttttta cattgctatg atacaatatt tttaacaaga ttttattatt
     1201 tgttaacttt tttcacagaa aggagaaaat catgcgttat gatttcggaa aggtctataa
     1261 agaaatacgt gagtcaaagg ggctgaccca agaagaggtc tgtgggggtg ttctctcaag
     1321 aaccagctta tcaaaaatcg aaagtggcaa gacaacccca aaatatgaaa atatggaatt
     1381 tcttctccgt caaatcaata tgagttttga agagtttgaa tatatctgtc aactttatca
     1441 accaagtcaa cgcacagaga ttatgcaaac ctatctcaat atgcgttcca ttatcgggac
     1501 cagcgatctt gtcaatttat ttcaaaaatg ccaagactac ctcaagaccc accacgatct
     1561 ccctatagaa gaaatcagag atatgcttga agttgtcatt tatatccgtc aacacggggc
     1621 aggggaacta tcagatcacg cagaacaggt tgtcaaaaaa ctttggagaa aaattgaaaa
     1681 acaagataca tggtatgaga gtgatctaaa aatcctcaat accattcttt tcagttttcc
     1741 cattgaatac ctccatctca tcactggaaa aatattgcaa cgcctagaag tctataaaaa
     1801 ctatcaacat ttatatgact tgcgaatagc aatcctgctc aacctttcca cactctatct
     1861 atacaatcaa gataaaaaca tgtgtaagca aatctgctac actttactgg aggacgccaa
     1921 gaataagaaa agctacgata ggctggctat ctgctatgtc cgcattggga tttgtacaga
     1981 cgattctaaa cttatccaaa aagggttctc ccttctggag ctgaccgagg aaacttctat
     2041 gctgtctcat ctcaaaaaag aagtagagac ccattatcaa ccaaagaaat tataaaaaaa
     2101 gtcgagggag ctcctcgacc ttttcataga atcgccgaac gatttaacga gaaagtatga
     2161 cttttacgtt tatcccaact caattatgac atttttttca aaagtcaata tatctcactt
     2221 tttcaacgac aagaaagagg ctgccaatcg accaacctct tattctgaac ccatcactcc
     2281 atcacttttt agcttcattc gctttcttag cgactgcaat ctggtattcg acttggtcaa
     2341 accctgtgcc tcccaaggaa tttcgtcttt gaacagcagt tttaggttgc aagtagacat
     2401 agatatcctc ggtaatgaga gaatgatagg tttgtaattc ctccaaagtc caatcttgaa
     2461 gatttttagc agacttgata gagtctagca ctaatcttcc cacaacctca tgagcttctc
     2521 taaatggcaa gcctttccct gccaaataat ctgccaactc agttgcattt gaaaagtcct
     2581 tctctgttga ttcttgcatt ttttccttgt ttacctgcaa actagataac atacctgcca
     2641 acacatcgag ggaatttaaa atcgtttcga ccgtatcaaa cattccttcc ttatcttctt
     2701 gcaaatcctt attataagct aaaggcaagg acttcatgac tgtcaacagt ccaaacaaat
     2761 gcccataaac tctgccagtc tttcctcgaa tcaactcagc catatctgga tttttctttt
     2821 ggggcataat agaggaacct gttgtaaacg tatctgacaa ggtaatgaat tggtactcaa
     2881 aactacacca attgatcatt tcttcgcaaa aacggctcat gtgcatcatg agtatgctgg
     2941 catttgacag aaattctaag ataaaatcgc ggtcactaac agcatccaaa gaatttgtat
     3001 aaggtttctt aaactccagc aaatcgctag acaattggcg atcaatgggg aaagtcgttc
     3061 ctgccaaagc tgccgcacct agagggcata ggtctgtatg cttctggtta aattcaaaac
     3121 gttcactgtc tctctgaaac atattgtagt aagccatcag gtgatgggca aaactaatag
     3181 gctgggcatg ttgcagatgg gtatagcccg gcataatagt ctccacatga ttttccgcca
     3241 agtctaataa aacacctttg agatgaacca gtttatctag gacatggcct agttgttcct
     3301 tgagatacaa gtgcatatct gtcgcaactt ggtcatttcg agaacgagct gtatgtaact
     3361 tccctgcaag gggaccaatt ttttctgtca acaacacttc catattcata tgaatatctt
     3421 cgtttgcgat atcaaagtca agttggcctg cctctagctc ttctaaaagc tctttcaagc
     3481 ctgcttggat cttttctgac tcctccaaac tcaaaatgcc tgtctgaccc aacatctgaa
     3541 cgtgagctaa agaaccaatc aaatcaaatt tagctaattt ctggtcaaaa gatatactcg
     3601 caccaaagcg ctctacccag tcttcgacag taccttcaaa gcgaccaccc cataattttg
     3661 tattcttagc catatcacat ccacctctct atccaatcgt cctatttggc gcttttttga
     3721 acctcagaat gaaccttggt tggaagtccc cacaacttaa taaagccaac agctgcatct
     3781 tggtcaaagg tatctgcact agtatacgtc gccaaatttt catcataaag tgaatttggt
     3841 gatttccgag ccacgacctg agcgcttccc ttatagagtt tgacttttgc agtcccattg
     3901 acaacttttt gagtctcttt aatataagca atcaaggctt gcgtagccgg actaaaccac
     3961 aaagcattat aaatgagatt tgataattca ttttctatga ttggtttaaa atgagccact
     4021 tctctcacaa gcgtcaaatc ctcaatttcc ttatgagctg tcagcaaggt cactgcccct
     4081 gggcattcat agatttctct tgatttaatc ccaactaagc gattttccac gtggtcaatc
     4141 cgtccaaccc catgtttccc agctatttca ttgagttttt gaatcaaatc tgccaatttt
     4201 aacacttctc cattgaggga aacagggaca ccttcactaa actcaatatc aatgtattct
     4261 ggcatatctg gagccgcctc tggagaagat gtgatgccaa aggcttcttc tggagcctga
     4321 ttccatggat tttccaaaat accacattca ttggcacgtc cccaaagatt ttggtcgaca
     4381 gagtaaggat tgtcaaggtc agcaggaaca ggcaccccat tttccttggc ataataaatt
     4441 tcttcctccc gagaccattt ccattcacga actggagcaa tcacttttaa attgagatcc
     4501 aaggctgcaa tcgatacttc aaaacgtact tggtcattcc ccttaccggt acaaccatga
     4561 gcaattgtag tcgctcctat ctgatgcgct atttcaacca attttttaga aatcagaggg
     4621 cggctcaagg cagataccaa gggatacttt tgttcataat aggcatgtga ctgatgagcc
     4681 actagcacat aatctgtagc aaattcgtcc ttaacatcaa tgacataaga ttcgactgcc
     4741 ccaaccttaa gagccttatc atggatgaaa tctaagtctt tcccttcacc cacatccata
     4801 caaactgcaa caacatcata gtctttcttc agccaggtaa tagcaactga tgtgtccaaa
     4861 ccgccagaat aggccaaaat tactttttct ttactcatgt tcttttcctt ctttctattt
     4921 ttcaatggat gctttaaagg catcctcaat gagtttgtct aattccccag actccttcaa
     4981 cttttgaatg gttttatcga ctgcctcttt caattccttg ctatcttttt tcatggctac
     5041 cgcataggaa tcatcttgct ctttttcaaa attgaggtct gcgattgcta aatcaggatt
     5101 attttccaca aatcccttgg caacgggttc ttcaaaaata acggcatcca cttgccctga
     5161 ttttaaatct gtgattaaat tcccattttt aggcagagat acgagggaag aattttgtag
     5221 caaatctttc gccatcgtct cttgaatcga acctttctgc gctccgacct ttttctgcgc
     5281 caaatcgttg acagactgat aagtggctaa atcagatttt ttgacaatga gtttattttt
     5341 tgcagtatag tagggagttg aaaaatcaaa caccttgctc cgttcatctg tcttagaaac
     5401 acctgatatg gcaaggtcag cttttcctga ttgaacgcta gccagtacat tgtcaaaact
     5461 cataggagat agttccaatt cgacacctag ttctgttgcg atagccttgg ctaattcgat
     5521 atctgaaccc acaatctgat ttttcccatc aaccactttt tgatattcaa atggagcaaa
     5581 atctggattg agggctacaa ccaattttcc tttggacttg atggcttcaa ttccagccga
     5641 ttgattgtta ctacaagcaa atagtagggg gagcatcact aaaccaaaca tcgtcatcaa
     5701 caccttcttc atcttattca taacaaagcc tcctttatgt ttattctttt ttattgtata
     5761 aataatactc ctatactcat cctttgtcaa gcctaaattg aaaaaatttt caatttgaaa
     5821 caaaaaaaac ctagaaatac tacataaaat gtcattccta ggcgtattta tgctattttt
     5881 ctctagaaaa catgagtatt cagccagtca aaaggagctg aatgaactca ttttccctcg
     5941 cccaattcaa tgacacgatg acattgttgg gctacataag catcgtgggt cacaataatg
     6001 actgttttcc cttctcgatt catctctaag agaaacttca agaccaaatc tctattttca
     6061 ggatccagcg aacctgtagg ttcatcggct aaaatcagct ggctgggttt taagatggct
     6121 ctagcaactg caattcgttg ttgttcgccc ccagacaact cggagaccct ttgatgcaaa
     6181 gtagctgaca aacctactct ctctaaaatc tcttccacct ttttgagctt gtctttctta
     6241 ggcaatttca catatttcag cgccagcatg agattgtact cgaccgtttc atcatcaatc
     6301 agggcaaaat tttgaaacag ataagagata tgttcacgga ttattgtttg cgacttagca
     6361 gaattaaccg ctagatttgt ctgaccaaaa atctcatacc gtccgctata atcaccatct
     6421 atcaaaccca ataaatttaa caaggtcgac ttcccactac cactcttacc aacaatagct
     6481 accaaatccc cctgatcaat cctgagagat aagttatcca aaatcacttt tcccccaatg
     6541 gttttggtaa tatttttcaa ctcaatcata agatgccccc tttcaataac tctactagac
     6601 ttcttttctc catactagaa gctaagccta gcacaaatag tatatccaga catgtaaaac
     6661 ctgcaaacag tagaagtggt aagaacgcat gggcaaagaa aatcaagact agaagaggga
     6721 aactatagcc cagcaagagc agaacgagga gaggacggta gcgatcgacc agtttccacc
     6781 ccataaactt cttggtaatg atatccctgc gcttcaataa gaaagttgtt actagtaaga
     6841 agtaggaaat catcatgcta aggagaccaa acaaagcaaa gagtaggtta aaattccgaa
     6901 cagcatctcg ataagaatcc actttctctt gttgaatggc ttgaatagat gaaaatttta
     6961 aataatttcc atctgacaat ttctcaacta actctgtaat ctctttttga tgttgaaccg
     7021 tattttcaat tttaatcgga ttatttaagc cagttgttga cagggaggct ttctcatccc
     7081 acatcatatc agaatcattg accaagctaa taattggatt ggagagattt tcctttcgct
     7141 tatcactata tgggaaaaat gaccaatctc cttcataata ggcaatctcg acatccatct
     7201 cctctatcgt tcgtttttgc tgctcttcat acttcatcga atgaaaggca attaacttcc
     7261 ccaagagctg atttttatct tcttcacctt tcgtacttgc tggcatcaaa ataacttttt
     7321 taataccggt atttggtagc ttgaatccct tgctctttag aaaattgcga ttggcatagt
     7381 aaacatccac cgtatctgtt aactgatatt gctgaatctg ttctgattgg acaaaatttt
     7441 ttacaggaag actgctactc tgcacatagc ccgcctgcgt tttttctacc aaatcctgat
     7501 aaaatcgata gaaataatct gtagatttcc ctgaccctgc tagctcttct tgccacagat
     7561 tatcattgag tttgaaggtt tctaaggtca ggtaattacc ttgacttacc cactgttgct
     7621 gataagcaag ttctttgttt tcttgttcta aacttctgcc caccccaatc agtaaggccg
     7681 tcagtaaaat agttgtccct attttcatca cataattgaa gataagacca aatttgaaag
     7741 atgaaaaacc tttcagcaga gagctgattg tcattttttg gattaagagg taagtcaacc
     7801 aactgataaa gagataaagc tgcaacagca aaaaatgaga caaccacagc ataggaaaca
     7861 aatcttttgg cttataatca agcaagaaaa acacgcctag attgatcaca agagtcccac
     7921 ctaggaggag gtaaaggttg ccttttacaa catcagctaa aacagcccta tcttgaaaac
     7981 caagtaattt ttgtacccca actcttttca tctccatcat cggttgatac actgtcacta
     8041 acacaagaag caaaatagcc aagacaaaaa caatggcaga taaaagcaaa tctcgattta
     8101 tgacttccac tgcacttttg taggtcggct ctagcaaggt agcctggtct atcttgaaaa
     8161 aatcgctcca tttctgtaca atcctatcct tgtccatctc ttgtgtagaa gttatcgtat
     8221 agcgaccatt taaactacga gatgtatcct tgatataggt ttgaaaagtc ataagctgaa
     8281 taggtttggc ttttagaaag gtcggaatcg taccaagttt attggaaatt tctttattac
     8341 tatagactcc ttcaccatct gtggtaaaat caagagaaga aatcccaaac tcttggtagg
     8401 ggaaggtatc tttatcaaaa acaccagact tgaccacctc atcaccactg tctgttttga
     8461 taatggagac tttatactcc tttgatacat cctcaaaaaa tcgaagaaca gacgctgcag
     8521 gttcgttaat atctttcaaa tacaaatcca aagaatctac agtctttccc atttcttgaa
     8581 tccgaaccac ttctcgtgta tagtttacat taaaaacaaa aaaagtggta cacagaagca
     8641 ggagtaacat acagaaatta ctgatttttt tcataaaagt gcctccacta cagttttccc
     8701 tctccaaata ggagagggaa gaattttact caaatccata gaaatattcc attcctgttg
     8761 gcggaatctt ggtgagggaa gctctagccc atgcctcagg ttttgcataa tccttagagg
     8821 ttctaccccc atgtctaaca gttgctgtat gatatcgagt agaatgtaag taatcagaat
     8881 atccaaaagt tcctgtccaa cctactccat agttccattg accaccctca acccatactg
     8941 ctaaagcatt tgtagtagca agtataccac ataccattaa agatgcaaca ccaattttaa
     9001 ctaattttct catgatttat ttctcctatt atcgagagct gttccgtaaa aacagctata
     9061 gtaatttatc aacaagtatc gtagtccatt ggactcacct tccttctatc tcatttcatt
     9121 ttttaacaac gaacttactc ttaaccggga gaatggtgcg cttctgtatc aggtccttga
     9181 acaaccagag tagtattaag caaactctaa ctattactca agaatcagtc caataaacaa
     9241 tatggatagc gctttcaaat ttttaaactc ctatcccgtc cttttatcta tataataagc
     9301 ataagcgaaa atataataac tgtcaagtat ctgaagtgaa ttttataaaa aaattacaag
     9361 ccaaatttgt aaagtttaca ctaagccgct agggcaatcg tctatcagaa tgtccgttta
     9421 tttgtcaata atccgagaaa atcttgcaac gcttagaagt ctataaaaac tatcaacgtt
     9481 tatatgactt gcgaatagca atcctgctca acctttccac actctatcta tacaatcaag
     9541 ataaaaacat gtgtaagcaa atctgctata ctttactgga ggacgccaag aataagaaaa
     9601 gctacgatag gctggctatc tgctatgtcc gtattgggat ttgtacagac gattctaaac
     9661 ttatccaaaa agggttctcc cttctggagc taaccgagga aacttctatg ctgtctcatc
     9721 tcaaaaaaga agtagagatc tattaccaag cgaaggaaag ataaaaaagc cgagggattt
     9781 cctcgactct ttacatatta tttgttcgtt agttcttaat accagccatt gttaagccag
     9841 aagtttttag cagcagtcca tgaaccgtaa cgtcctgcaa cgtaggcatc tgctacacgt
     9901 tcttggtttt cagctgagta gtcaccgttc aggtatgaat ctgttaattg gtaacgtccg
     9961 atataacgtc catttgtagc tgtatagcta ccacctgatt ctttttgagc gatccattct
    10021 ttggcttctg cttcagatcc gcttacagtt gaaacaactg tttcacttac tactggagtt
    10081 tctgctactg gagctgaaac agtttcatct tgagcggctg gtgccgcata agtagctggc
    10141 gctggtgttg caacaggcgc tacagggcca tcgataacca actcttgatc aacataaatc
    10201 aaatgaatgt tatcaatgtg gttgttttct gccaattttt caactgttgt gttgtgagtt
    10261 tcagcgattt ctgaaagtgt atcaccttct ttaacagtgt aagttgatga ttcttgagca
    10321 gatacaaatg atggagcaaa tactgcaaac aaggcagcta ctcctgcaag agttgcttta
    10381 atctttttag ttattgattt catattcgaa aattctcctt ctttctatag ttctatcata
    10441 ccctttaaat attaccgttt cttgacactt ttatgtagaa atattacaaa attatttttt
    10501 atttccctaa ataaggtgtt ttttgtcata aaagatacta tttttatgct atttgaggta
    10561 ttagaaaggt tttcatccac aattaaagcc aagcccctca ttctttgata taatagagat
    10621 aagaattttt ataaggggaa actgatgaaa tcacttcgtt ttcaatctgt ctttgatatc
    10681 atcggaccag ttatgattgg cccatctagt agccataccg ctggtgctgt tcgtattggg
    10741 aagattgtct cttccatttt tgatgatact ccgacagaag ttgaattcca actttttaac
    10801 tcatttgcca agacctatcg tggtcacgga acagacctag cccttgttgc aggtatttta
    10861 ggaatggata cagatgatcc tgaaattcca aatagtctgg aaattgccca caagcgtggt
    10921 atcaagattg tttggaccat tcagaaagac agcaatgccc ctcaccctaa caccactaaa
    10981 attactgtta aaaatgccca caagaccatc agcgtgactg gtatttctat cggtggagga
    11041 aatattcagg taaccgaact caatggtttt gccgtctctc tcaatatgaa tacaccgact
    11101 atcatcatcg ttcatcaaga tattccaggt atgattgccc ttgttacaga ggcgctttcc
    11161 cgctatggta tcaatatcgc ccagatgaat gtcactcgtg aaaaagctgg tgaaaaagcc
    11221 attatgatta tcgaagttga cagtcgcaac tgtgatgagg ctatcgaaga aattcgaaaa
    11281 atccctcatc tccacaatgt caatttcttt aaataggagg aagcatgttt tattctatca
    11341 aagaattggt cgagcaagca gatctggact ttcaaggaaa tgtcgcagaa ctcatgatta
    11401 caacagagtt tgaattgacc ggtcgcgaac gtgaagaagt cttccttctc atggaacgca
    11461 atctggaagt tatgaaagcc tctgtccaac ttggcctcaa tgaaaataaa tctcgtagtg
    11521 gcctgacagg tggagatgct gccaaattgg atcactacat cgaaaacgga aaaactctgt
    11581 cagattatac gattctctcg gctgcccgaa atgccatcgc agtcaatgaa cacaatgcca
    11641 aaatgggctt ggtctgtgcc actccaaccg ctggaagtgc tggctgtctg ccatccgttc
    11701 tcactgctgc tattgaaaaa ttagacctca gccacgagca acaattggat ttcctctttg
    11761 ctgctggtgc ctttggacta gtcatcgcaa acaatgcctc catctcaggt gctgagggtg
    11821 ggtgtcaagc tgaagttggt tcagcctctg ctatgagtgc tgccgccttg actctggctg
    11881 caggtggaac accttatcag gccagtcaag ctattgcctt tgtcattaaa aatatgctag
    11941 gcctcatctg tgaccctgtt gcaggtttgg tcgaagttcc ctgtgtcaaa cgtaatgcca
    12001 tgggagctag ctttgctttc atcgcagcag acatggcctt ggcaggtatc gaatctaaaa
    12061 tccctgtgga tgaagtgatc gatgccatgt accaagtagg agcaagcatg ccaactgcct
    12121 ttcgtgaaac agctgaaggt ggactcgcta ccacccctac tggtcgtcgc ctccaaaaag
    12181 agattttcgg agaataagct tatcaaatag gagaaaccat gacctctatc acagcgattt
    12241 ttttcgattt ggatggaacc ctcgttgata gttctatcgg gattcacaat gcctttacct
    12301 atacctttaa agagctgggg gtgcctagcc ctgatgccaa aactattcgt ggttttatgg
    12361 gaccacctct cgaaagtagt tttgcgacct gcctgtccaa agaccaaatt tctgaagcca
    12421 tgcagatata tcgttcttac tataaggcaa aaggcatcta tgaagctcaa ctctttcctc
    12481 agattataga cttgcttgag gagttatcga gcagttatcc actctacatc accacgacaa
    12541 agaatacatc tactgctcaa gacatggcca aaaacttgga aatccatcat ttctttgatg
    12601 gtatttatgg ttctagccct gaagcacccc ataaggcaga tgtcattcac caagccttac
    12661 agacacatca actagcacca gaacaagcca tcatcatcgg agataccaag tttgatatgc
    12721 tgggagctcg agaaacaggt attcagaaat tggccgtcac ttggggattt ggagagcaag
    12781 cagatctact aaactatcaa cctgattata tcgctcacaa acccttagaa gttttggcgt
    12841 attttcaata gcatagactg ggtaacaact ccatttcaga agaaaatgaa gtaatactca
    12901 atgaaaatca aagagcaaac taggaaacta gctgcgggct gctcaaaaca ctgttttgag
    12961 gttgcagata gaactgacga agtcagtaac catatctacg gcaaggcgac gttgacgtga
    13021 tttgaagaga ttttcgaaga gtataagtct ctacataata aaacgcatat taccaaggtt
    13081 gtttaacttg ataatatgcg tttttctttt ttattcaatg tgggttgttt gattagcaaa
    13141 ggcgataata gcttgcttca actcatctcc actgagagtg cggaactctc caatcttttg
    13201 attgatggat tctaaaggca tgacattaac cttaccaacg aaaatcttat ccataacttg
    13261 attgtcaacg agttcggttg ataccaagag ttcttcgtag agtttttcac ctgggcggat
    13321 tccaacttca acgattggaa tttcactttc agtgtggcca cttagaagca ccatcttctt
    13381 ggccaagtca taaatcttga ctggtttacc catatcaagg ataaagactt ccccatcttt
    13441 ggcataagca ccagcatgga taaccagacg gctagcttct ggaatggtca taaagtaacg
    13501 ggtcatgcgg aagtctgtca ccgttacagg cccaccttca gcaatttgac gttcaaagac
    13561 tggaatgaca ctaccacggc taccaagaac attcccaaaa cgaactgcac agtaggttga
    13621 ttggctacgt tggttaaagc cagtgacaat caactccgcc acgcgcttgg ttgctcccat
    13681 aacatttggt ggattgactg ccttatctgt cgaaatcata accatcttag gcaccttggc
    13741 ttcatcaacg gctctagcaa cattgtaagt tccacggata ttgtttttga aggcttcttt
    13801 tggattgcgc tccatcatag gaacatgctt gtgggctgcc gcatgataaa caatagcagg
    13861 tttgtactgc tcaaagactt gcaacaaacg atcatagtct tgaatgtccg caatcacagg
    13921 tacataatca atcccttgga acttacgaat caattcatga taaacaaggt agattgagtt
    13981 ttccccatga ccgagcaaga caatgcgttc aggattgaag cgactaactt gacgacagat
    14041 ttcagaaccg attgaacctc cagctcctgt gactaagatg gtcttacctg tcagttctgc
    14101 acccagacgc gattcgtcaa gacggatttc ctgacgaccc aaaaggtccg taatatcaat
    14161 tttttggaag ccagtacctg cttggtgaag gccctgaaca acagtttcaa ccttaggcat
    14221 cttgtaacat ttgacaccca gcttattaca catctgcaag atacgctcat attctgacgg
    14281 atccagcgac ggaatcgcaa cgatgacacg ctcgatttga tggcgtttgg ctaattcagg
    14341 cagattgtca taagagccca aaacaggaat accaccaagt ttttgaccct ttttcttaga
    14401 atccttatcc aaaataccga ccagttctaa ttcactggtt ggatgttggt aactatccat
    14461 aaagagagcc ccaccatcac cggcaccaat caagaaggtc cgacggtgtt ctccatcacc
    14521 actacctttt ttgcgtctgg agtagattaa ctgccaagta atccgtggca ataaaatcaa
    14581 gaaggtactc aacaagataa agagaatgat gaaacggatg gagaagagtg gcaagaaggc
    14641 ataacagata ctatatgaca agacactgct agcagtcaca ccaaaaaaga ttttcatgaa
    14701 atccgtaatc ttgctgtaac gactaatgct cgcgttcaac ccccaaaaac caatcatcaa
    14761 ttgatagaac aagaaggcca aactcgtata gataatgtag tcaacaggtg ctggattaat
    14821 cagcccataa aataaaatat aagatacaat tatggaaacc accatactga aaatatcaaa
    14881 tattccccag aaaacctgct tttgttgttt atttaaaatt tccaccagat caatcacata
    14941 atctgttagt tttttattca tagaaatcta ctcctcctta aaagagtttt actatattgt
    15001 tattgtagca tattttgatt tatttggctt attttatcca aataaattat tgttcccttg
    15061 tactccttct tccatttaaa tcctctaatt aaacctacaa tcgtcccaag gccataagca
    15121 aagtgaatgg aaaataaaag aaagggcatc acaattagaa atccattttt atgttttaat
    15181 aaagtcagca aagtgagtag tgacaaaagt agaaaatagg cacctaatag taaagttatg
    15241 aatacgaatg tgatcggtaa caatgctaga ctaaacacaa gactcaaaac aaataaacaa
    15301 ggaacatagt gaaataatga taaacactta aactgaacat gacttgtcaa gccaatccac
    15361 aaaccatttg aatacttttg atgcagcatt ttcttgaatg ttggtcgaat atactgataa
    15421 gatagaatac ttgggctata gcggatttta taaccatgtt ctcgaattct ataatgaata
    15481 tcattatctt cagttcggcc aagttgctca tttactaaac caaccttctg gaaaacctct
    15541 cgtttataca tcccatgaaa aatagaagaa acatatctat cctcggaact atttcgataa
    15601 ttggcaatac tactgccaaa catattttcc tcaacaagat gcaaggtctc tgcccatttt
    15661 ccttttcctt cgacaatcgt cggtctaggc cccccacaga caaattcacc ttgttgaata
    15721 atagccacat tgttcattac aaaactctca gtaacttttg aatgagcatc aatttttaaa
    15781 ataaggtccc ctacagaatg tttaactccc aggttaaaac cactagcttg atttttctta
    15841 ggattgttat acaatctaat tgagttaaac tctgtatctt cctttataaa ttgctgaatg
    15901 atagctgtgg tcccatctgt ggacatagca tttataaata gaatttcaat attctcttta
    15961 ggataagttt gattttttaa gtcttcaatt agaccaggca gatatttttc ttcattgtat
    16021 gcactgatta caatggatac gagaagactt tcaacatttt gagtcactac tactctcctg
    16081 aatttttcct tccaccattc tatcataatt ttcaagactt tcccattttt atcttgaaag
    16141 ccagaaacct tacttgacat tatagtgaaa aagccttaaa ataagctgtt attaaaatca
    16201 gctagaagag gtattatatg aaaaagcaat cactcttttt tgttccaggt attatcctga
    16261 ttggtgtttc cttgcgaact ccttttactg ttttacccat tattttggga aatatttcgc
    16321 aaggtctgga ggtagaagtt agttcgcttg gtgtcttgac cagcctgcct ctccttatgt
    16381 ttaccctctt ttcaccattt tctacccaac tggctcagaa aatcggcttg gagcatctct
    16441 tcacctacag cctcttcttc ttgaccatcg gctcacttat tcgactaatc aatctgcccc
    16501 tgctctatct aggaaccttg atggttgggg caagtgtcgc agtcatcaat gtcctgcttc
    16561 ctagtcttat ccaagccaat caaccaaaga aaattggttt tctgaccacc ttatatgtaa
    16621 cgtctatggg gattgcaacg gctctggctt cctatctagc tgtgcccatt acacaagcca
    16681 gttcttggaa aggacttatc cttctcctca cgttactctg tctagcaact tttttggtct
    16741 ggctcccaaa tcaccgctat aatcatagac tagctccaca aaccaaacaa aaaagtcaaa
    16801 taaaggtcat gcgtaataaa caggtttggg caattattat cttttcaggt tttcaatcct
    16861 tgatctttta caccgtcatg acctggttac ctaccatgtc tatccatgca ggtctatcca
    16921 gtcacgaagc tggcttgctg acttctatct tatctctgat tagcattcct ttttcaatga
    16981 ccatcccaag cctgacaacc agtttatcta ctcgcaaccg tcagctcatg ctcactctgg
    17041 tttcactagc tggtgtggtc ggcatttcca tgctcttttt cccaatcaat aatttcattt
    17101 actggcttgc catccatctc ctcatcggaa ccgcaaccag tgccctcttc ccttatctca
    17161 tggtcaactt ttcactcaag acaagcgccc ctgaaaagac agcccaattg tccggcctat
    17221 ctcaaacagg aggctatatc ctagcagcct ttgggccaac cctctttggt tacagttttg
    17281 acctgttcca ctcttgggta ccatctgtag ctgccctctt gctcatcgat atcctgatga
    17341 ctgtggccct ctttacagtg gacagagcgg ataagatcct ttaaacttct agttttagct
    17401 agaagttttt tatatataaa aattttacac atttctcttg acagggcttt cttttagatg
    17461 tacaatgtat gtgtagaaaa attatatata aaaatcttac acattagaaa aggaggtttc
    17521 ccatgtactt tccaacatcc tctgccttga ttgaatttct catcttggcc gtactggagc
    17581 agggtgattc ttatggttat gagattagcc aaaccattaa gctaatcgct aatatcaaag
    17641 aatccacact ctatcccatt ctcaaaaaat tggaaggcaa tagctttctg acaacctatt
    17701 ctagagagtt ccaaggtcgc atgcgcaaat actactcctt gacaaacggt ggtatagagc
    17761 agctcttgac cctaaaagat gaatgggcac tctatacaga caccatcaat ggcatcatag
    17821 aagggagtat ccgccatgac aagaactgaa tacctgactc agctagaact ctatctcaag
    17881 aaactacctg aagctgaccg tatcgaagcc atggactatt tcagagagct ctttgacgat
    17941 gctggagtcg aaggagaaga agaactcatc gctagtttgg gaactcccaa agaagcggcc
    18001 cacgaagttc tatccaatct tctcgataaa aaaatcaatg aagcacccgc tcaaaaaaat
    18061 aaccgacaaa ttttacatat cgccttgtta gccctccttg cagcacctat cggcattcct
    18121 ctgggaatcg ccatcctcgt gaccctgttc gcaatccttg tagccgcttt gactgtcatt
    18181 ctggctttct ttgcagtttc catactgggt atcatcggcg gattcctatt tttagttgaa
    18241 agtttcacta tcctcgccca agccaaatca gcctttatct tgatttttgg ttctggttta
    18301 ctggctatcg gtgcttcttc gctagtttta cttggcattt cctatgtagc tcgcttcttc
    18361 ggtctactca ttgttcgtct ggtacaattt gttcttaaaa aaggaaagag aggtaatcag
    18421 catgcgtaaa tggacaaaag gatttctcat ctttggtgtg gtgactaccg ttatcggctt
    18481 tatcctgctt tttgtaggta tccaatctga cgggattaag agcctacttt ccatgtccaa
    18541 agaacctgtc tatgatagcc gtacggaaaa gctaaccttt ggcaaggaag tcgaaaacct
    18601 agaaattact ctccaccaac acgcgctcac catcacagac tctttcgatg atcaaatcca
    18661 catttcttac catccatctc tttctgctaa ccatgatctt atcacaaatc aaaacgatag
    18721 aactctgagt ctcactgata agaaactgtc tgaaactccg tttctctctt ctggaattgg
    18781 tgggattctt catatcgcaa gtagctactc tagtcgtttt gaagaagtta ttctccagct
    18841 accaaaaggg agaactctaa aagggatcaa catctcagcc aatcgcggac aaaccaccat
    18901 cataaatgct agccttgaaa atgcgaccct caatacaaac agctatatcc tccgaattga
    18961 aggaagtcgt atcaaaaaca gtaaactcac aacgcccaat atcgttaata tctttgatac
    19021 agttcttaca gatagtcagc tagagtcaac agagaatcac ttccacgctg aaaatatcca
    19081 agtccatggc aaggttgaac tgactgccaa agattatctc agaatcatcc tagaccagaa
    19141 agaaagccaa cgaattaact gggacatctc aagcaactat ggttctatct tccaattcac
    19201 aagagaaaag cctgaatcaa gaggtacgga attaagcaac ccttacaaaa ctgaaaaaac
    19261 cgatgtcaag gatcaactca ttgcgagatc tgatgataat attgatctaa tatccacacc
    19321 aagcagacgt tgacaaaaac gcttacattt gctactatgg aaacatattt atctaactaa
    19381 aaaggaggtt ctaccatgaa acaagaatgg tttgaaagta atgattttgt aaaaacaaca
    19441 agcaagaaca agcctgaaga gcgagctcaa gaggttgcag acaaggctga agaaacgata
    19501 gccaatctcg atacaccaat tggaaaaaat actcaagtag aggaggaagt ctctcaagct
    19561 gaagtcgaac tggaaagcca acaagaagag aaaattgaag ctcctgaaga cagtgaagcg
    19621 agaacaggaa tagaagaaaa gaaggcatct aattctactg aagaagagcc agacctttct
    19681 aaagaaacag aaaaagtcac tatagctgaa gagagccaag aagctcttcc tcagcaaaaa
    19741 gcaaccacga aagagccact tcttatcagt aaatctttag aaagtcctta tatccccgac
    19801 caagctccaa aatctaggga taaatggaaa gagcaagtgc ttgatttttg gtcttggcta
    19861 gtggaagcga tcaaatctcc tacaagtaag ttggaaacaa gtatcacaca cagttacaca
    19921 gcctttctct tgctcattct gttttctgca tcttcctttt tctttagtat ctatcacatc
    19981 aaacatgctt actatggaca tatagcaagc attaacagtc gcttccctga gcagctagct
    20041 cctttaactc ttttttctat cgtctctatc ctagtagcga caacactctt cttcttttca
    20101 ttcctcttgg gtagtttcgt tgtgagacga tttatccacc aggaaaagga ctggacgcta
    20161 gacaaggttc tccaacaata tagtcaactc ttggcaattc caatcttcct cactgctatt
    20221 gctagtttct ttgctttctt tgatagccta cgatttacag ccctcttgtg tgtgattagc
    20281 attggaatca ttctgcttgc cagtctccat atcattacaa gaccaagtca agcaagtgaa
    20341 accgactcct tctatcaatt attcttgtct gtccttgtga acggagttat tatcctcctc
    20401 ttctttgtag ctgaagtggc actgattgga gattatcttc gtatcttggc ctttctttaa
    20461 aattcaatcc tgtcattctt ggcaggattt tttctatgtc aaaaaagcaa gtcgattgac
    20521 ctgcttctgc tttacttttc ttatgctaat gttttaaaat cttgtactag gatgggttgt
    20581 atttctattt gattggcgtc tagctggtgg taaaaagatg ggggtaacac ctctaagaat
    20641 tttgcatagt ccaagcgagc tatggattca tagtcttctg gtttagagcc gtctcctgtg
    20701 atttgaacca agtcaatctc ccactggact tccttatcca ccaattgctc aatataggct
    20761 gcaggaacaa acggttctct cggtaaaact gtcttgactc cttgagctag atggtaaacg
    20821 ccatgcactt ttccttctcc atcctgatag aaggaaactc tcgcaaagta ggcatctgtc
    20881 tcttgaaccg cacgcacacg cgcttcaaac tgagccaagc catctgcctc taaaaagctt
    20941 ttcaagttct cacgactaaa gaaaatagga ttaaaaattc cttggcaaat cgtaaaacga
    21001 gctacagggt ctgctagata ggcttgaata cttgcttgga aataagcttc aaaatcaaaa
    21061 ccatctagtc cttcaatatc agtccctgta taagcaagca aagcatctaa aaatgacttg
    21121 ataatctgcc agtctgtctt tgttagtagg tcaggaacat cgacacgata agccttttga
    21181 ccatcagcat aactgacctt aaagagaaat tgtgattgcc ccactatcgc acactcgata
    21241 tattcgagac gattaagagg ctgacggaga tagactacat catagctatg tgactccaaa
    21301 ccgtctagca aggccaagat cgacttggca ttcaaaatct cttgttctcc taaaatgctc
    21361 tgtttatttg gaataaaaaa tgttttcgcc ataactggac tcctttgaaa tcgtttacat
    21421 acagtatacc ttatttttct gaaagataca aaaacaaact ttagattttt gagtacaaag
    21481 catagaaaaa cagcccctga ggactgtttg atcttataca gtagctgctt gatccaagct
    21541 ttcaccgata gcggctaggc gctcgatcac ttcagcttgt gtcaattcat tttttgaaac
    21601 atagcggtta cgtgggtgaa cacggcactc gtgtgagcat ccacgaaggt acttgtcttc
    21661 attttcttct gatgtcaaga tacgacggtt acagaaggga tttccacagt tgacataacg
    21721 ttcacatggt gttccatcaa accagtcttt ccctacgata gttgggttga catggttgac
    21781 atcaacggca atacgctcgt caaagacgta cattttccca tcccaaagct caccttgaac
    21841 ttctgggtct ttaccgtaag ttgcgattcc tccgtgcaat tggccgacat ctttgtaacc
    21901 ttcacgaacc atccagcctg agaatttctc acagcgaacg ccacctgtac agtaaaccac
    21961 gacacgcttg tccatgaatt tttccttgtt atcacggacc cattgtggta actcacggaa
    22021 gttgcgaata tctgggcgaa tagccccacg gaaatgtcct aggtcatact cataatcatt
    22081 acgtgtgtca aggacaacgg tatctttatc aagaagcgct tctttgaact cttttggaga
    22141 caagtaagca cctgttgttt caagtgggtt gatgtcattg tcaaagtcgt tgtcttccaa
    22201 accaaggtgg acaatttctt tcttgtagcg aacaaacatc ttcttgaagg cttgttcact
    22261 ttcttcgtca atcttgaacc agagttcttc cattcctgga aggctgtgaa cgtagtccat
    22321 gtatttttga gttgtttcat agtcacctga aactgttccg ttaattccct cgtcagcgac
    22381 taggatacgt cctttaagac cgattgattt acagaaagcc aagtggtctg cagcaaattg
    22441 ctctgcattt tcaattggag tataaaggta gtaaagtaag acacgaatat cttttgccat
    22501 aagatttgtt ctcttttcta ttcttaaatt ttctaaattt tttatttatc tacactagta
    22561 tacctgcttg agaaaacgaa tgcaatttat ttgactggaa attgtagagg gttctcgaaa
    22621 tttcctgaat ggttaaaata aggacaagag aaaacatgga tatctatatc cttgtgccaa
    22681 aaaaaccact gtctccccca gaccaacctg aggaaagcag tgattcttat tttaggagtt
    22741 aggaatgaat acacgaaatc aatttagctg attatttttt gtttttcaag aattcatcgt
    22801 attgtttttg catttcgttc aatacttttt cgtaggcacc ttcagatttc aatttttcca
    22861 tcaattctgg aatcgcttta tctgggtcta cagtaccagt gttgatagct gtatcaaatt
    22921 gttgcattgt gttagcaata gctgagattt cagatttcac attgtcagta ttgaagataa
    22981 atccaagcgc tggagattct ttagcttctg ccaattcttt cttagaattt tcgatttgtt
    23041 ggtctgtaac gttttcgttg atgtaaagga tccagttgtt accagtgttc catccaccca
    23101 tgtgagtgtt tcctttgtag ccatcaagaa cgcgaacacg gttttcttta ccttcaattt
    23161 tttcccagtt cttgccttct ggaccgtaaa caagaccgtt caagagttct gggttcgtat
    23221 tcaagaggtt caagatttcc attgattttt ctttgttctt agagttgttt gagatgacaa
    23281 agttagcaac ttgtgttgtt tggtttttct tgatgaagtt agtaattggt ttgatttgga
    23341 tatctttgtt ggcaacacgt gaaagcaagc tgttaccgta gtcagctggt cctactgttt
    23401 cttcacgaac gaaccaagta tcttgttgaa ggtcaaatga agtgtcactt gttgcgacgt
    23461 cttttggaat gtagccagct tcatagaatt tgtgaagagt cttcaagtgt tctttgaaac
    23521 gaggcacttc gtaacggttt acaactttag tagtatcgcc ttcaaggtca ataacgaatg
    23581 gaagcccgtt tgctactggg tagtcaaagt tatcagatgg gatgaaaact ttaccaatag
    23641 caaatggtac tacgtctgga gctttttctt tgatttgttt caagactggc tcaagagttt
    23701 cgtaagaagt aacacctgaa atatcgatac catatttggc aaggagagtt ccgttgaagg
    23761 caaagttttg agatgatgct acgttggctg caactggtac agcgtaaatc ttaccattta
    23821 cagtattacc cttgatataa gctggatcaa gtgccttgta aaggtcttta ccttcttttt
    23881 tgtacaattc tgtcaagtca gcataagcac ctttttgagc atttacaata tagttatctg
    23941 caaaggcgat atcatagttt tcaccagatg atgtgataac tgacattttc ttaccatagt
    24001 caccccagcc aaggtattgg atatccaatt tggcaccaac tttttcttcg atgattttgt
    24061 tggcatttgc taacaattca tccaagttat ctggtttgtc accgatttgg tacattttga
    24121 taacagattt gtcacctgaa tcagcagctt ttttgctgtt acctgtcaag ttgccacaag
    24181 cagcaagacc tgcagccaaa gcgactacac tagcagatgc aaaagcatat tttttccagt
    24241 ttttcatgat aaaaactcct ttttttattt ttaaacttat aaacaatgta atgatcttaa
    24301 cttcacgcaa gggaagtttg gaaatgagaa atgttctttt tcaataagca ctattctttc
    24361 acaccaccga tagtcaaacc ttttacaaag tagcgttgga agaatggata caaaatcgcg
    24421 attggaaggg ttgctaccac aaccatggcc atacgacctg tttctttcgg tagagcaact
    24481 cccagttgac cagtcaagcc gaccgctttg gcaatgtagt ccatattttg ttggatttgc
    24541 atgagcaaat attgcaatgg atacaagtta tcactcttga tgtaaagaag ggcgttgaac
    24601 cagtcattcc agaaaccaag agctgtcaaa agcgtgatgg ttgcgatacc tggtagtgac
    24661 aatggcaaac agatttggaa gaaaatccga gcctcactgg caccatcgat acgagccgat
    24721 tcgagaatgg cttctggaat ggtcttcttg aaaaaagaac gcatcaagat gatattgaat
    24781 ggtgagagaa gcattggaac aatcaaggcc caaacagtgt caccaagctg aagtacacgg
    24841 gtcaccatga tataacctgg taccaaacca gcgttgaaca acatactgag aaggacgaag
    24901 atggtaaaaa atctgcgata cttaaaggtt gtccgtgaaa tagcgtaggc ataggttgtt
    24961 gtgataaaga catttgtcaa tgtcccaact acggttacaa agacagagat gaagagggct
    25021 tgtaggattt tatccttaaa ctgtgccaaa aactcaaaac cgtctaagcc aaattgggat
    25081 gggaagaagc tatagccgta ttggaggagg cttttctcgt ctgtcactga aataatgata
    25141 acgaatacaa aaggtaggat acaagagagg gcaatcaaac ccgaaatgat actgaagaag
    25201 atatctgctt tcttactgaa ggagtgaatg ccgacattat caattttttc ttttttaatt
    25261 ttcttttttg ccatattctc ctcctttcta gaacaaagct gagtttggat cgactcgtct
    25321 tgcaagcaag tttgatagga taaccagaat caaaccaaca acggattggt aaagaccggc
    25381 tgctgcagcc ataccgatat ctgctgtctg agtcaaacca ttaaagacat atacgtccaa
    25441 aacgttggtt acattgtaaa gctgaccagc attgtgtggg atttgataga agagaccgaa
    25501 gtctgcgcgg aagatatttc cgactgcaag gatggtcaat acagttacaa gcggagtcaa
    25561 ctgaggaatg gttacattgc gaatacgttg ccacttgcta gctccgtcca ctgtcgctgc
    25621 ttcgtagtag gttggatcaa ttcccatgat cgtcgcatag tacatgacac tgctatatcc
    25681 aaagcctttc caaataccta ggaaaagtag gagatagggc cagatgccca ggtcagcgta
    25741 gaaattgact tctttgagac caagactttc caatagatga ttgaacaccc ctttatcaat
    25801 atttaggaag gcatctgtaa agaaactgat gataacccaa gacaagaagt aagggaacaa
    25861 catagaagtt tgaaaaatct tcaccattct cttagaacgg agctcgctga ggataatggc
    25921 aatccctaca gatacaacta aacctagaaa gataaagcca agattgtaga ggacagtatt
    25981 tcgtgtgata ataaaggcgt ctcttgaact aaataagaat ctgaaattat cgagtccgac
    26041 ccatttacta tttatgatac tatctatgaa accattactg gtcatgtggt agtctttgaa
    26101 ggcaaccacg ttcccaaata ctggaatgta aaagaataga atcaaccaga gtgcccctgg
    26161 caaaaccatc aagagaaaga tccagttgtc tctcaatgtt tttgaaaact ttttcataat
    26221 ttcctccctt tttattttga tatccatcta aaaattcttt tttagacttt tgataacgat
    26281 tacattatta gtatactcct atttgcaggt taggttaaac tcctaattat agaaaaaact
    26341 ccacaaatta tgtagcagat ttaaaacttt atcaccacta tcaaacaaat gtcctaaatc
    26401 aattgtttat tttatctcta ttagcccagt gatggcgtca ctctgttata agcatccaac
    26461 aacggggtat actgaaaaat ctccagacta gggaactcag cgatagttcc taatctggag
    26521 atttttaata tgttattagg cgtttgcttt caacttagca ataacctctt taagattatc
    26581 aatcaactct gctgcagtat gctcagagcc tttttcatct gccaagaaca aaactgcttt
    26641 ttgaagttct tcttgagagt tttcaaggac atccttatct actgtttcaa ggtttgagtc
    26701 tttaagaagt ttacttaatt ccttggctaa tttcttgagt ttgatttgca gactcatctt
    26761 ctcctgctgt ttctttgccc gctgtttgtc ctccatcctt agttgctgac tggctttcct
    26821 taatggactc tagggaagca atggcatctt tgactgtttg caagatatca cgtaaacctt
    26881 gctctatcaa actatcatct gcaaaagctt tcttagagtc tgccaaaacc agacgtgctg
    26941 aatctgtggt aggattcgat acacctgtca atgatctcaa aagattttct aaggtttgag
    27001 tctgcttact aatactagac taaaatcaaa aagcattata taacagtgat atgaaatcaa
    27061 ctaaagaaga aatccaaacc atcaaaacac ttttaaaaga ctctcgtaca gctaaatatc
    27121 ataaacgcct tcaaatcgtt ctatttcgtc tgatgggcaa atcttataaa gagattatag
    27181 aacttttaga ctgtaatcaa acaacgattt ggcgaaatgt aaaaaaatat gaggagttcg
    27241 gactcgactc tctccttcaa gaaacacgtg gtggtcgtaa ccatgcatat atgacagttg
    27301 aggaagagaa agtctttctt gcccgccatt tgaaggctgc a
//
LOCUS       DAVKJJ010000028        26083 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000028 DAVKJJ010000000
VERSION     DAVKJJ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26083
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.028"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(48..845)
                     /gene="psrA"
                     /locus_tag="VW144_001211"
     CDS             complement(48..845)
                     /gene="psrA"
                     /locus_tag="VW144_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEU9600663.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            complement(902..2470)
                     /locus_tag="VW144_001212"
     CDS             complement(902..2470)
                     /locus_tag="VW144_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9600664.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRFSTLVEIVRG
                     GSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
                     NSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVV
                     KNLNSDKVASILIPLPPLAEQQRIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKK
                     SILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNS
                     YYEEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLAR
                     FIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYGWAVADSHVTVIRV
                     LSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIPLPPLPEQSRIV
                     DKIEQFFAHIDALI"
     gene            complement(2470..3933)
                     /locus_tag="VW144_001213"
     CDS             complement(2470..3933)
                     /locus_tag="VW144_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9600665.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSIM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            complement(3946..6279)
                     /locus_tag="VW144_001214"
     CDS             complement(3946..6279)
                     /locus_tag="VW144_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9600666.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFRPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKEDSNWRKVIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDTNGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQSNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGEPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            6886..7395
                     /locus_tag="VW144_001215"
     CDS             6886..7395
                     /locus_tag="VW144_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEU9600667.1"
                     /translation="MNSRVEFRIFTILDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQEFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTATFLGGLVVCMSLFREELSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            7559..8593
                     /gene="hrcA"
                     /locus_tag="VW144_001216"
     CDS             7559..8593
                     /gene="hrcA"
                     /locus_tag="VW144_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU9600668.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKVLQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFHRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRIADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQINVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            8620..9144
                     /gene="grpE"
                     /locus_tag="VW144_001217"
     CDS             8620..9144
                     /gene="grpE"
                     /locus_tag="VW144_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU9600669.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            9624..11447
                     /gene="dnaK"
                     /locus_tag="VW144_001218"
     CDS             9624..11447
                     /gene="dnaK"
                     /locus_tag="VW144_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU9600670.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            11458..11862
                     /locus_tag="VW144_001219"
     CDS             11458..11862
                     /locus_tag="VW144_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HEU9600671.1"
                     /translation="MDEEYLKNTRKVYNDFCNQADNYRTSKDFIDNIPIEYLARYREI
                     ILAEHDSCIKNDEAVRNFVTSVLLSAFVSALVSAMISLEIQTYKFVIPFIIGMIWTVV
                     VFLMINWNYIANTKKRQKYINISVLIGYLKSK"
     gene            12206..13342
                     /gene="dnaJ"
                     /locus_tag="VW144_001220"
     CDS             12206..13342
                     /gene="dnaJ"
                     /locus_tag="VW144_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU9600672.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGDGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGQGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(13709..13996)
                     /locus_tag="VW144_001221"
     CDS             complement(13709..13996)
                     /locus_tag="VW144_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600673.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            complement(14006..14416)
                     /locus_tag="VW144_001222"
     CDS             complement(14006..14416)
                     /locus_tag="VW144_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9600674.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            14484..15215
                     /locus_tag="VW144_001223"
     CDS             14484..15215
                     /locus_tag="VW144_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9600675.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            15212..16261
                     /locus_tag="VW144_001224"
     CDS             15212..16261
                     /locus_tag="VW144_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9600676.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYLISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVEVVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(16429..16758)
                     /locus_tag="VW144_001225"
     CDS             complement(16429..16758)
                     /locus_tag="VW144_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU9600677.1"
                     /translation="MTDTDPIKRAQTLITDLNKAYQACKQATADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            17035..17373
                     /locus_tag="VW144_001226"
     CDS             17035..17373
                     /locus_tag="VW144_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9600678.1"
                     /translation="MKHMIIQTQKTVYKVNVDDVYYIQTHPTKAHTVKIVTEEASFNM
                     VQNLSNLENQYGETLMRCHRNCLVNLDKVKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            17378..18115
                     /locus_tag="VW144_001227"
     CDS             17378..18115
                     /locus_tag="VW144_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9600679.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKEHHITLSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANGQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSTRPHRVILYTKTDRLEFTASLEEVLKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            18129..19469
                     /locus_tag="VW144_001228"
     CDS             18129..19469
                     /locus_tag="VW144_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9600680.1"
                     /translation="MNVAWILLYTLVTHGLKIVIFFKVDGISLTFERIFKAFLFKILL
                     AVVFGMLGYMVGNVYLSYFMEPLYGIGLSFLLLRELPKKLLLFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDDYSFIWLCIIIFNFFISLAFLKWLDYDFTSLRKGILDKDFQK
                     SLTQINWIMGAYYLVIQNLSYFEYEQGIQSTTVRHLILVFYLLFFMGIIKKLDTYLKD
                     KLHERLNQEQDLRYREMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EIYDSVLKDSSEKLQDNKYDLGRLVNVRDRALKSLLAGKFIKARDKNIVFNVEVPEEI
                     QVEGVSLLDFLTVVSILCDNAIEASVEACQPHVSIAFFKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQDHVFRQVLTVIHIE"
     gene            complement(19513..19668)
                     /gene="blpC"
                     /locus_tag="VW144_001229"
     CDS             complement(19513..19668)
                     /gene="blpC"
                     /locus_tag="VW144_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEU9600681.1"
                     /translation="MDKKQNLTSFQELTTTELNQIIGGGWWEDFLYRFNIIEQKNTKG
                     FYQPIQL"
     gene            complement(19725..21086)
                     /locus_tag="VW144_001230"
     CDS             complement(19725..21086)
                     /locus_tag="VW144_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEU9600682.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVFIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEITPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSSEDEFGYHNTFMNFTKQSHDIELGISKTN
                     TEVSNQANLTNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LIASQGQTQGTAEEPFLSQINQSIASLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     SIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            complement(21097..23250)
                     /gene="blpA"
                     /locus_tag="VW144_001231"
     CDS             complement(21097..23250)
                     /gene="blpA"
                     /locus_tag="VW144_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter BlpA"
                     /protein_id="HEU9600683.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFSEWTGVAIFLAPKPSYQPHKDKKNGLLSFLPL
                     IFKQKSLIAYIVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVITYIL
                     QQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDANSII
                     DALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLLSIPIYMFIIFSFMKPFEKMNH
                     DVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYSILQT
                     SLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQTKLQS
                     AKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDINLTIK
                     QGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINYQDIKNIDKKVLRRHINYLPQQ
                     AYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAGLSGG
                     QKQRIALARALLTKAPVLILDEATSGLDVLTEKKVIDNLISLTDKTILFVAHRLSIAE
                     RTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            23532..23786
                     /gene="blpM"
                     /locus_tag="VW144_001232"
     CDS             23532..23786
                     /gene="blpM"
                     /locus_tag="VW144_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HEU9600684.1"
                     /translation="MNTKMMEQFHEMDIAMLSSIEGGKNNWQTNVFEGGSAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVALWAGVTGATGGF"
     gene            23802..24005
                     /gene="blpN"
                     /locus_tag="VW144_001233"
     CDS             23802..24005
                     /gene="blpN"
                     /locus_tag="VW144_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HEU9600685.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGKILGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            24249..24398
                     /gene="blpO"
                     /locus_tag="VW144_001234"
     CDS             24249..24398
                     /gene="blpO"
                     /locus_tag="VW144_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEU9600686.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            24502..24621
                     /locus_tag="VW144_001235"
     CDS             24502..24621
                     /locus_tag="VW144_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEU9600687.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            25089..25460
                     /locus_tag="VW144_001236"
     CDS             25089..25460
                     /locus_tag="VW144_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600688.1"
                     /translation="MIKKNHILCEIVPGSVAFACFYLSISISSVFQFLLVVINLASIL
                     VTVYLTREMRIKEFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITAGESYQQIIAYI
                     GAILCLTIMLLLIWGLKYYKK"
BASE COUNT         7786 a         4668 c         5167 g         8462 t
ORIGIN      
        1 cataatcaca aaaatagcag gtagtcagtt tgaccacctg ttatttttta ccaattaaca
       61 attttatcta caatattttg ttgttcagta gctgttttcc ttagataaat tcgagtagtt
      121 tctatacttt cgtgtcccat caaatctgca agcaaggcaa tatcattata cttcgctaaa
      181 aaattcttag caaataaatg cctaaaagaa tgagggtaaa ttactttagg attcattttg
      241 tatttatcag cataattttt taactgttga gcaactcctc ttgctgtaat tggttcgtta
      301 aatttattca aaaataaata accacttcgg cgattttctg attctaacca actaagacaa
      361 ctatttctta attttttagg aatgtacagt ctacgaattt taccaccttt tgagtaaatg
      421 tcaaaataac cgatttctac atgctctact tttagtttaa taagttcact tacacgagcc
      481 ccagttgcac ctaaaaacca aacgacaaaa tgccatttta aaataccatc ttttttcaaa
      541 ctacgtttaa gaaaaaggta atcagcatgg ctaatgacat cttctaaaaa tggtttttgc
      601 tgtactttga caaattttaa tttcaaatca tcatgaccaa taaaagccag atatttattt
      661 actccttgta gtcgcaaatt gacagtttta ggtttaaaat tgtctaataa atatcctttg
      721 tattcaaata aatcttccat tttgagttcg taattctcca agaaaaatcg aacaccataa
      781 aggtacgaac gcacagtatt ttcagctaaa ccagctttct tcaaatgtaa ttcaaaatct
      841 ttcaacgtaa aactcctatc ttatgtttga tagaaattcc accgcacgta aaactattat
      901 actaaattag tgcgtcaata tgggcgaaaa attgttcgat tttatcaacg attctggatt
      961 gttcaggaag gggtgggagg gggattaaat attcttttat agttttcgtt aataattctt
     1021 tttgttttgt actacccgac gctttttctt caataactga ctgaacaata ggagaggaaa
     1081 gaaaattata gatgaaatgg caattaataa ctcccgataa gactcttata actgtaacat
     1141 ggctatctgc aacagcccag ccataaggat ttttattttc atggtaaata gctaatcgtc
     1201 ctaacgtacc tagacctgtt gaattccaca ttaaatcacc atctcttagt aatctttctt
     1261 tctggtaact atgaactgtt tcgggatcaa taaatcttgc taagtcaata gaaaagccag
     1321 accattgatt acatttctga gcaatcacag ggtatatagg aatatttgaa tattttggag
     1381 acttccctct ttgaatgtag gaggttatat cgtttaacct cacccattcc caactttctg
     1441 gtatttcaca aggtacttcc tcataataag agttatcatc tccttgggaa acaatagaaa
     1501 tgtccaaatc tttcttttta atcttgcctt cttcaaagag tttttgtttt tctgctcgta
     1561 ttttttcaag taaaacttcg actgattcat catttgggtc ttgttcaact aattttcctt
     1621 gcatagcata ttgaagaata gattttttta gtttatctgg aaattcttta tctagctgtt
     1681 ctagtctatt ataactttca gcatattcat ctactttttc taaagctgat tcgattgctt
     1741 ctattatgcg ttgttgttca gctagcggag ggagagggat aagaatagaa gcaactttat
     1801 cactattcaa gtttttcaca acagctccac taattagaga tagaaattga gaataaacta
     1861 catttgatga aagaatatag aataggtaat ctttatttaa tgagttttca tagttcgaaa
     1921 tagccaacca tccatcgtgt attgcaccat caacattcaa aatataaggt ctaccaaaac
     1981 tcatagaatt agttaacaaa aatgtacctt tttttacaaa tctagttttg ttaagccctg
     2041 attttttgat tttttcttta acattattta tatacttttc acccttttca gtatcaccta
     2101 tttttatcca atttattcca tctacttcag aagtaagata atctttgatt ggtcgtggag
     2161 agccacctct gacaatttca accaatgtag aaaacctcac ccactcccaa gtatcaggaa
     2221 tatcataagg aacatcaatt tcttgagtgc ttccatcagc aaacttccca taatgtttcc
     2281 catcatcacc acgaaatatc tcagtttcct ttttatctcg tttgattttt ccttcactga
     2341 taagtttttc tttttcagct ttaattctct ttaataattc acttgcaggt tcgtcattgg
     2401 gattttgcgg cactaatttc ccttccatcg ctctttggag aatacttgcc ttaagttgtt
     2461 ctggtgtcat tatttgtcct ccaacaactg caaaatatca gctaatacat tatcaatctt
     2521 atgatttaaa gttgctcttt ccgcttgata attctgaatc aactcaaagg gatttaagat
     2581 ttcctcttcc tcttttggaa aaccacactg gtctaaatta taattcaact cagccaattc
     2641 actaggtgta aatgatttag atttgtagaa cttaccttcc agaatctctt cacgattttc
     2701 ccaccagtca cgaacaggat tgaagtgttc tgacttcatc ggcttagttt tcgagaaatt
     2761 tttataacca tctggcatat ctaaacgata aaaccaagtt tcttctgttt tctttgtttt
     2821 atcaaagaaa agaatgttcg tatggattcc tgtatacggt gcaaagacac tatgaggcaa
     2881 cctaataatc gtatgcaagt tgaactcatc taccagtttt tgtttcaagc gagtttttac
     2941 accttcacca aatagaaaac catcaggtaa aataactcca acacgaccat tttctttcaa
     3001 acgatacata atgacagcca taaataaatc agctgtttca gaactccgta attctgctgg
     3061 aaagttattt tttattgttt ctaattctga ccctccaaaa ggtggattca tcataataat
     3121 gtcaaatttt tcatcatccg tatattcacg aacatttttc tccaaagtat ttccatgaac
     3181 aattttaggg tcatcaattt cgtgaagaaa cagatttgta actgctaaaa gatgaggaaa
     3241 tgctttcttt tcaataccaa aaacagctgt attatatttt ttggtatctt cactagtttt
     3301 acgttgacta cttaaacggt tcagagtcga agtcaagaag cctcctgttc cgcaagcaag
     3361 gtctgccatt gattctccaa gttttgggtt aagaacttcg gcaataaaat cagtcgctgc
     3421 acgtggcgta taaaattctc ctgagttccc agcattttga atatctttaa gaattttttc
     3481 gtaaatatca ttaaacgaat gacgatcttc agggctattg aaatcaactt catcaataac
     3541 attgatgact tggcgtaaca agacgccatt tttcatatag ttgttcgcat cttcaaaagc
     3601 tgatttaaca atcgtttttc gaataggcat aattgaagtt atttcaagct ctttcaactc
     3661 tttgaataac ttgttattga caaaatcaag taattcatcg cctgtcaata cccgttcccc
     3721 attttgagca tgagcccaat ttcgccattt taattcctct gggataattg actcatactc
     3781 gtcttcttct aattcccaaa ccatttcacg gctatcataa atttttaaga ataataacca
     3841 agacatttgc tcaatacgtt gagcatcacc attaacacca gcatcgtttc gagtgatatc
     3901 ttgaattctt tttacaaatg atgtaattga catactttcc tttcattaag ctactgtaaa
     3961 tagctcttgc tccaattctt taattgcttg taaatatcgt tttttatctc caaaataagt
     4021 attgatgatt ttgaaggttc caccatatat ctgaaattct ggaagtttta atgtttcaat
     4081 gctttcgagt tctccaatac ctttatccat gtatttgttc agtaaaattt ccaaaacagc
     4141 acgcgcttcc tcactatatt tatataaata tccgctttgt ttgagtttat tgatacgttc
     4201 cgttttggtt aattcttttt gaccataggc aagttttagg agtaaatcaa aatcatcgat
     4261 ttcttgttct gatattccct ccgactctcg aatagcatct agataaactc cttttttata
     4321 aagttcgtct aagataagct tctttttatc cgccgtatgc caaactgtga taaaatcgtt
     4381 caaagtggcg tagctaccta agatattctt ccgagtgtag tcggtcaggc tttcggtaat
     4441 cagtttcccg ttttcatcca atacttgaac agtagaatta agaatggtaa cctgcttgtc
     4501 tgtaacgata tatttttcta ctgggtcacc ttcctcatct acgaaaccgg gcgtagaacc
     4561 actgactgtt ttcgcacctg tttccagcac cttcactggc tcaccatcaa aatcagggtc
     4621 agcaaacaaa ttggtaacat ttcgaaaatc aataatcgta aaaaattctt tccccttttg
     4681 aggataaaga cgtgtgccac gaccaataat ttgtttaaat tcagtcatgg attggatatt
     4741 agagtctaaa acaatcaaac gacatgtttt agcattaact cctgtcgtta ataatttaga
     4801 cgttgttaca atagcgggaa aattagaatt gacatccata aagttatcca gttgagcttt
     4861 tccttcagcg ttgtcaccag ttacttgcat gacataacga tagtcttctt ggactaagtc
     4921 tagattctct tttacaagtg cagcacgcat tcgctcggca tggtcaatat caacacaaaa
     4981 aacaattgtt ttatcaaatc gtgcattgct ttgcttcata taatcagaaa caaacttggc
     5041 aactctttgc gttctatcat caatgacaat ggttttatca aaatctttcc tgccgtagta
     5101 cctatcttct attaattgtc cgttagtatc aacttttcca gtttctggac gataaccatc
     5161 cacatccaca tctaaattaa ccctcataac acgatatgga gccaaaaaac catcctcgat
     5221 tccctgtttt aaactataag tatagattgg ctcaccaaag tattccgtat tggaagcatt
     5281 cttggtttct ttaggagtag cggtcatccc aatctgtgtc gcagaactga aataatcaat
     5341 caccttacgc cagttactgt cttccttagc tgaaccacgg tgcgcttcat caattacgat
     5401 taaatcaaag aagtctttgt caaatttttg ataatgtgtt tcagttccat cttcaccagt
     5461 tagttgctga taaagcccta gataaatttc aaaagaattt aatttttcag gagcagtcaa
     5521 aagttttggt gtaattttcg tcattacctt ttcgaatggc ctaaagtctt cagccatcgt
     5581 ttggtctact aagatgtttc tatctgctaa gaataaaact cgtttagcca aaccagcttt
     5641 tcgaaggcga tgaataattt gaaaagccat gaacgttttc cccgttcctg ttgccatcac
     5701 aaacattact cgtttttgtc ctctggcaac tgtttcaata gtacggttga tagctatttg
     5761 ctgataatag cgtggcgttt tcattgagaa ggcgtctgta tagtatggag ttgagatagc
     5821 ttctgtaatt tcgtacgtca atcctttttc cttcgtcata cgagaaaata attcttcacg
     5881 agtagggaat tcgtctaact ccagctcacg ttcttctctc gtgatacggt cgtgttcaat
     5941 aaagccatca ccattcgaag aataaacaaa tggaacatct aaaatctctc catattcaat
     6001 agcttgttgt aatcctgctc gaacgctgtg tttattatcc tttgcctcaa caattgcaat
     6061 tcgagttcca aattggtaat acagtgaata gtctgatttt tttccttctt tacgacgagc
     6121 cttatctcct ctaacttcaa ttcgaccatc agtgaagtat tcttcgtaag cgatatgctc
     6181 accatttttc catcctttgg atacaatagc aggagtgatg aaatttgctt taatatcctc
     6241 ctcactcatt tctttcttag actttataaa ctcaaccata ttgaaccctc cgcattctaa
     6301 aaatctactt ctccataaaa taaactatca gaacaaatta tacttctatt atatcacact
     6361 tataaaaata ctctgctaaa ttgttgaaaa taagtatgca atatactttc gcatcgcgtc
     6421 agattatctc cgcataacac cgctattttt cgctgttttc ggcaggcgac ttattatcat
     6481 tctataagca ctttcagtcg ggctggtgag ctatttttca aaaataaata atcacattta
     6541 aacattttga aagtataaaa tatctacatc agccgttcaa aaactggcgt aaattttgcc
     6601 ataatatttt cgtttaaata aagatttttt atgccaaacg gattttaaca atgcggatgc
     6661 ttcctatttt gcttctcttt ttagctctac taccggtttt ctcaaagttt gtcactggag
     6721 gtagttcttt cagttgagga gtatttttga ttttcataat agattgggtt ttattgatag
     6781 tttttgcgat tcagatttct tatatttttt ggagattgag tcaaaagtgg aaagaattat
     6841 ctaataaata acgccaggca tgttttctaa ttttggggta agacaatgaa tagcagagta
     6901 gaatttcgga ttttcaccat tcttgatttg gacaaggaag aagaatattt acatgagatg
     6961 catttgaaag gttggaggta tagaacgagt cgttttggtt tgttctattt tgaccaatgt
     7021 caaccagacg atgtcatcta ccgtatctat gattctagat ttcttaaaaa atataagcat
     7081 gaactgcaag aatttagaga tagaggttgg gaattgatag gagcaggttc ttgttcgatt
     7141 cttcgtaaat cgtcttctga tttacttcca gaggatcaag tctatatgag taaggggctc
     7201 aaatgggaag ttatgcgatc tagacttcgt tcctgtacag ctactttctt aggtggtctt
     7261 gttgtttgta tgagtttgtt tagagaggaa ctttctatgt ctttctttct tatttttgtt
     7321 ttatatgctt ttctgatttc ttatctaatt catggttatt tcagactaaa aaggaaatac
     7381 cgagtagatg aatagcaagg ttctaggtct tcagattgat ttttagcact cttgataaaa
     7441 gagtgctaat tttttgagtt tttgtcttga cattctcttc taagggtgta taatagaatc
     7501 ataagttagc acttggatgc attgagtgct aattgatcag acagagagga gtgatgagat
     7561 ggttacagag cgtcagcagg atattttaaa tctgattatt gacatcttta ccaaaacgca
     7621 cgaacctgtc ggatcaaaag tcttgcaaga gtctattaac tctagcagtg caaccattcg
     7681 taatgacatg gcggaactag aaaaacaagg gttgcttgag aaggctcata cttcaagcgg
     7741 tcggatgcca agtgttgctg gttttcagta ctatgtgaaa cactcactgg attttcaccg
     7801 gctggctgaa aatgaggtat atgagattgt caaagccttt gatcaggaat tcttcaaatt
     7861 ggaggatatt ctgcaagagg ctgctaactt gctaacagac ctgagtggct gtacggtagt
     7921 ggcactggat gttgagccga gcaggcaacg tttgacagcc tttgatatcg ttgttttggg
     7981 gcaacataca gccttggcgg tatttaccct agacgagtcg cgaacggtta ctagtcagtt
     8041 tctgattcca aggaacttct tgcaggagga tttgctgaaa ctgaagagca tcattcagga
     8101 acgtttcctc ggtcacaccg ttttagatat tcactacaag attcggacgg agattccgca
     8161 gattatccag cgttacttta caacaacgga taatgtcatc gatctctttg aacacatctt
     8221 taaggaaatg ttcaacgaaa acattgtgat ggcgggcaag gtcaatctct tgaattttgc
     8281 caatctagca gcctatcagt tctttgacca accgcaaaag gtggccttgg agattcgtga
     8341 ggggttgcgt gaggatcaga tgcaaaatgt tcgtattgca gacggtcaag agtcctgttt
     8401 agctgaccta gcggtgatta gtagtaagtt cctcattcct tatcggggag ttggaattct
     8461 agccattatc ggtccagtta atctggatta ccaacagcta atcaatcaaa tcaatgtggt
     8521 caaccgtgtt ttgaccatga agttgacaga tttttaccgc tacctcagca gtaatcatta
     8581 cgaagtacat taagattgaa atcattaaag gaggcgaaca tggcccaaga tataaaaaat
     8641 gaagaagtag aagaagttca agaagaggaa gttgtggaaa cagctgaaga aacaactcct
     8701 gaaaagtctg agttggactt ggcaaatgaa cgtgcagatg agttcgaaaa caaatatctt
     8761 cgcgctcatg cagaaatgca aaatatccaa cgccgtgcca atgaagaacg tcaaaacttg
     8821 caacgttatc gtagccagga cttggcaaaa gcaatcttac catctcttga caaccttgag
     8881 cgtgcacttg cagttgaagg tttgacagat gatgtgaaga agggcttggg gatggtgcaa
     8941 gaaagcttga ttcacgcttt gaaagaagaa ggaattgaag aaatcgcagc agatggcgaa
     9001 tttgaccata actaccatat ggccatccaa actctcccag cagacgatga acacccagta
     9061 gataccatcg ctcaagtctt tcaaaaaggc tacaaactcc atgaccgcat cctacgccca
     9121 gcaatggtag tggtgtataa ctaagataca aagcccgtaa aaagctcgca gtaaaaatag
     9181 gagattaacg aagtgttcga tgaacacaag aaaatctatc ttttttactc agagcttagg
     9241 gcgtgttcga ttcggcaatt ctgacggtag ctaaagcaac tcgtcagaaa acggcaatcg
     9301 ctatggcgtt tgcctagctt ccttactaac tcgtcgtcga aataaaatcg atttcgactc
     9361 ttcgtgtcgc aatttacata atagaaaact tgtccgaaac gacaataaac tatgaagaaa
     9421 gataaaatat gtttggcttt gtaatagtga gcgaagcgaa ccaaacacga tactcttcgc
     9481 cgtggcgcta tttgcgcaaa ttttgagacc ttaggctcaa agtttagtca aagagattga
     9541 cgaagtcaag ctctgacggc gtcgccactt aagaagagta tcaaaaagaa aaatagaaaa
     9601 ttaactaaca aggagaaaaa cacatgtcta aaattatcgg tattgactta ggtacaacaa
     9661 actcagcagt tgcagttctt gaaggaactg aaagcaaaat catcgcaaac ccagaaggaa
     9721 accgcacaac tccatctgta gtctcattca aaaacggaga aatcatcgtt ggtgatgctg
     9781 caaaacgtca agcagtcaca aacccagata cagttatctc tatcaaatct aagatgggaa
     9841 cttctgaaaa agtttctgca aatggaaaag aatacactcc acaagaaatc tcagctatga
     9901 tccttcaata cttgaaaggc tacgctgaag actaccttgg tgagaaagta accaaagctg
     9961 ttatcacagt tccggcttac ttcaacgacg ctcaacgtca agcaacaaaa gacgctggta
    10021 aaattgctgg tcttgaagta gaacgtattg ttaacgaacc aactgcagca gctcttgctt
    10081 atggtttgga caagactgat aaagaagaaa aaatcttggt atttgacctt ggtggtggta
    10141 cattcgacgt ctctatcctt gaattgggtg acggtgtctt cgacgtattg tcaactgcag
    10201 gggacaacaa acttggtggt gacgactttg accaaaaaat cattgaccac ttggtagcag
    10261 aattcaagaa agaaaacggt atcgacttgt ctactgacaa gatggcaatg caacgtttga
    10321 aagatgcggc tgaaaaagcg aagaaagacc tttctggtgt aacttcaaca caaatcagct
    10381 tgccatttat cactgcaggt gaggctggac ctcttcactt ggaaatgact ttgactcgtg
    10441 cgaaatttga tgatttgact cgtgaccttg ttgaacgtac aaaagttcca gttcgtcaag
    10501 ccctttcaga tgcaggtttg agcttgtcag aaatcgacga agttatcctt gttggtggtt
    10561 caactcgtat ccctgccgtt gttgaagctg ttaaagctga aactggtaaa gaaccaaaca
    10621 aatcagtaaa ccctgatgaa gtagttgcta tgggtgcggc tatccaaggt ggtgtgatta
    10681 ctggtgatgt caaggacgtt gtccttcttg atgtaacgcc attgtcactt ggtatcgaaa
    10741 caatgggtgg agtatttaca aaacttatcg atcgcaacac tacaatccca acatctaaat
    10801 cacaagtctt ctcaacagca gcagacaacc aaccagccgt tgatatccac gttcttcaag
    10861 gtgaacgccc aatggcagca gataacaaga ctcttggacg cttccaattg actgatatcc
    10921 cagctgcacc tcgtggaatt cctcaaatcg aagtaacatt tgacatcgac aagaacggta
    10981 tcgtgtctgt taaggccaaa gaccttggaa ctcaaaaaga acaaactatt gtcatccaat
    11041 cgaactcagg tttgactgac gaagaaatcg accgcatgat gaaagatgca gaagcaaacg
    11101 ctgaagccga taagaaacgt aaagaagaag tagaccttcg taatgaagta gaccaagcaa
    11161 tctttgcgac tgaaaagaca atcaaggaaa ctgaaggtaa aggcttcgac gcagaacgtg
    11221 acgctgccca agctgccctt gatgacctta agaaagctca agaagacaac aacttggacg
    11281 acatgaaagc gaaacttgaa gcattgaacg aaaaagctca aggacttgct gttaaactct
    11341 acgaacaagc cgcagcagcg caacaagctc aagaaggagc agaaggcgca caagcaacag
    11401 gaaacgcagg cgatgacgtc gtagacggag agtttacgga aaagtaagac gagtgtattg
    11461 gatgaagagt atctaaaaaa tacacgaaaa gtttataatg atttttgtaa tcaagctgat
    11521 aactatagaa catcaaaaga ttttattgat aatattccaa tagaatattt agctagatat
    11581 agagaaatta tattagctga acatgatagt tgtatcaaaa atgatgaagc ggtaaggaat
    11641 tttgttacct cagtattgtt gtctgcattt gtatcggcgc tggtatcagc tatgatatca
    11701 ttagaaatac aaacatataa atttgtaata ccgttcataa ttggtatgat ttggacagta
    11761 gttgtatttc ttatgatcaa ttggaattat atagcaaata ctaagaagag acaaaaatat
    11821 ataaatattt ctgtacttat aggatattta aaatcaaaat aaagttaatt tacttatttg
    11881 cagaggttgc aacccagcct ctgtttttcg ataaaaaggg acggaatctt atttgtttgg
    11941 gttttgtctc atcaatagaa aggaacaaag agtgttcgta actgaacacg ggtttcagaa
    12001 tttcttacta aatataaaag aaaggaattg aatccgacct aaatggtggt tcgattcaga
    12061 acatcaatag aaaggaataa gggtgttcgt aactgaacac gggctacgga ctgtgccaaa
    12121 aagatagttt tttctaggac ataagcgtcc gtcgtcaaaa ctcctagatg gctgtgtccg
    12181 tttgacgccc tttgtatctt gaattatgaa caatactgaa ttttatgatc gtctgggggt
    12241 atccaaaaac gcttcggcag acgaaatcaa aaaggcttat cgtaagcttt ccaaaaaata
    12301 tcacccagat atcaacaagg agcctggtgc tgaggacaag tacaaggaag ttcaagaagc
    12361 ctatgagact ttgagtgacg accaaaaacg tgctgcctat gaccagtatg gtgctgcagg
    12421 cgccaatggt ggttttggtg gagatggtgg tttcggcggt ttcaatgggg caggtggctt
    12481 cggtggtttt gaggatattt tctcaagttt cttcggcgga ggcggttctt cgcgcaatcc
    12541 aaacgctcct cgccaaggag atgatctcca gtatcgtgtc aatttgacct ttgaagaagc
    12601 tatcttcgga actgagaagg aagttaagta tcatcgtgaa gctggctgtc gtacatgtaa
    12661 tggctctggt gctaagccag ggacaagtcc agtcacttgt ggacgctgtc atggcgctgg
    12721 tgtcattaac gtcgatacgc agactcctct tggtatgatg cgtcgccaag taacctgtga
    12781 tgtctgtcac ggccaaggaa aagaaatcaa atatccatgt acaacctgtc atggaacagg
    12841 tcatgagaaa caagctcata gcgtacatgt gaaaatccct gctggtgtgg aaacaggtca
    12901 acaaattcgc ctcgctggtc aaggtgaagc aggctttaac ggtggacctt atggtgactt
    12961 gtatgtagta gtttctgtgg aagctagcga caagtttgaa cgtgaaggaa cgactatctt
    13021 ctacaatctc aacctcaact ttgtccaagc ggctcttggt gatacagtag atattccaac
    13081 tgttcacggt gatgttgaat tggttattcc agagggaact cagactggta agaaattccg
    13141 cctacgtagt aagggggcac cgagccttcg tggcggtgca gttggtgacc aatacgttac
    13201 tgttaatgtc gtaacaccga caggcttgaa cgaccgccaa aaagtagcct tgaaagaatt
    13261 cgcggctgct ggtgacttga aagtaaatcc aaagaaaaaa ggcttctttg accatattaa
    13321 agatgccttt gatggagaat aatactcttt gaaaatctct tcaaaccacg tcagcgtcgg
    13381 cttgccgtat atatgtgagt cgtatctaca acctcaaaac agtgttttga gctgacttcg
    13441 tcagttctat ccacaacctt aaaacggtgt tttgagcagt ctgtgcctag ctttctagtt
    13501 tgctttttga tttttattga gtatgagtta cctaaattat gatgcatagt tgatgggata
    13561 tatataatag attgaaatag aatatgaaca aattgataag aggattttaa agtaatctct
    13621 aacaatgctt tagaaactat ggtgtgctat tctaaattca attcactata acttgtttac
    13681 gttttaaaaa agagccgggg ggctcttttt acttatcttc agttccctgc atttctttta
    13741 tcacagctag tctagtctgg atatcctttt ccaagacctt aaacttgtaa gtcaagtctt
    13801 cttggtattc cttgataagt tctttttgct ggttaatgat ttgcaggctg ttttggataa
    13861 tatccacatc gtccttgata gcttgaacgc ggtcagtggt attcaagact tcatctgtga
    13921 tggtttggcg attttttgta accagataac ttccggctgc agctcctgca aatagcagta
    13981 ggttggataa tttcatagca actccttaag cgtttttgat ggtttcagcg acttgagcaa
    14041 gtttgtcaaa gtctggttcg tgggcgataa aatcaatctt gaggtcatcg tcagcactgt
    14101 agcgaggcac aaggtgaacg tgagtatgaa aaactgtttg accagcgact tcttcacagt
    14161 tggaaatgat attcatacca gcagccttag tgactttcat gactttttga gctacttttg
    14221 gtacttgggc aaagagttgg ctggcgctcg tagcatccat ctccaaaaga ttgcgatagt
    14281 gttcttttgg cacgaccaag gtgtgtccta gtgttacttg agagatatca agaaaggcaa
    14341 ggacctgctc atcttcatat acttttgaag caggaatttc ccctgcgatg attttacaaa
    14401 aaatgcaatc tgacataaaa tctacctcta ctgtactgaa ttttgatata atatagctac
    14461 attataccag atttggagaa aatatgttag aaattaaaaa cctgacaggt ggctatgttc
    14521 atgttcctgt tttgaaagat gtgtccttta ctgttgaaag tgggcagttg gtcggtttga
    14581 ttggtctcaa tggtgctggg aaatcaacga cgatcaatga gattatcggt ctgttggcac
    14641 cttatagtgg ctccatcaat atcaatggcc tgactctgca aggagatgcg actagctacc
    14701 gcaagcagat tggctacatt cctgagacgc ctagtctgta tgaggaattg accctcagag
    14761 agcatatcga aacggttgct atggcttacg gtattgagca aaaagtggct ttcgaacgag
    14821 tagagccctt gttaaaaatg ttccgtttgg aacagaaatt agactggttc cctgttcatt
    14881 tttcaaaagg gatgaagcag aaggtcatga ttatctgtgc ttttgtggtg gatccaagtc
    14941 ttttcatcgt ggatgagcct ttccttggtc ttgatccgct ggctatttct gatttgattc
    15001 agcttttgga agtggagaag caaaagggca agtctattct catgagtacc cacgtgctgg
    15061 attcggcgga gaagatgtgt gatgcctttg tcattcttca caagggagag gtgcgtgcca
    15121 aaggcaatct cctgcaacta cgtgaagcct ttgatatgcc tgaggctagt ttgaatgata
    15181 tttacttggc tctgaccaaa gaggaggatc tatgaaagac ttgtttttaa agagaaagca
    15241 ggcctttcgt aaggagtgtc ttggttatct gcgctatgtg ctcaatgacc actttgtctt
    15301 gttcctgctt gtcctgttgg gctttctagc ctaccagtac agtcaactct tacaacattt
    15361 tcctgaaaat cattggccta tccttttgtt tgtaggaatt acgtctgttt tacttttact
    15421 ttggggagga attgccacct atatggaggc tccagacaag ctctttctct tagttggaga
    15481 agaggaaatc aagctccatc tcaagcgtca aactggcatt tccctagtct tttggctctt
    15541 tgtacagacc cttttcttgc tgttatttgc gcccttgttt ttagcaatgg gttatggctt
    15601 gccagttttt ctgctctatg tgcttttatt gggggtagga aaatatttcc actttcgtca
    15661 aaaggccagc aaatttttca ctgaaactgg actggactgg gactatctta tttctcaaga
    15721 aagcaagcgt aagcaagtct tgcttcgttt ctttgccctc tttacgcagg tcaagggaat
    15781 ttcaaacagt gtcaagcgtc gtgcctatct ggactttatc ttaaaggctg ttcagaaggt
    15841 gcctgggaag atttggcaaa atctctatct gcgttcttat ctgcgaaatg gcgacctctt
    15901 tgctctcagt cttcgtcttc tcttactttc cgtactggcg caggttttta tcgagcaagc
    15961 ttggattgcg acagcagtgg tagttctctt taactacctc ttgctcttcc agttgctggc
    16021 cctctatcat gcctttgact accagtattt gacccaactc tttccgctgg acaaggggca
    16081 aaaggaaaaa ggcttacagg aggtagttcg aggattgacc agttttgttt tacttgtgga
    16141 agtagttgtt gggttgatta ccttccaaga aaaactagcc cttctagcct tactaggagc
    16201 tggtttggtt ttactagtct tgtatttgcc ttatcaggta aaacgtcaga tgcaggacta
    16261 acattgctga tacgacacta aaaaagaagt tgagttcagt ctgtctcaac ttcttttttg
    16321 ttactacagg ataatggttg gtccgtagag acttatactc ttcgaaaatc tcttcaaacc
    16381 acgtcagcgt cgtcttaccg tactcaagta cagcttgcgg ctagcttcct agtttgctct
    16441 ttgattctca ttgagtatta acttggtctt gacttggtca aagtggaagc ggtcataggc
    16501 ccgccaagcg gcgcgagttg gagcatctgg atcaagagcg ctgagtccca tgagaagact
    16561 ggaagtctgg taaaattttt ccaattcaat caagaatcga ttatccactg tttcagcctt
    16621 ggctagaaaa ccaagaatag agtttaattg ctcctgaaag cggacgtcgt cagcggttgc
    16681 ctgtttgcat gcttgatagg ctttgtttaa gtcagtaatc aaagtctgag ctcttttgat
    16741 agggtctgta tctgtcatga gaattcctcc tctaatctgg gtgccagtct tgattctagc
    16801 aactgtgttt tgatactgtc agtctatcac ttttatctcc tttttcgcct ttaaatcttt
    16861 aattgtcatt gaaatgtctt gaattgcgct gagtgaattt tatgataaaa tagtggtaag
    16921 ctcatcatga tattgtagaa aaataattct tttaggagct ttcaaagact gtttaggatt
    16981 aggtgtgctt gtgttagatt ttttctgtta ttcttttctt aggaggagaa tccaatgaaa
    17041 catatgatta ttcagacaca gaaaacagtc tataaagtaa acgtcgacga tgtctactat
    17101 atccaaacac atccaactaa agcccatact gtaaagattg ttacagaaga ggctagtttt
    17161 aatatggttc aaaatttaag taatcttgag aaccaatatg gggaaacctt gatgagatgt
    17221 catcgaaatt gtttggtcaa tcttgataaa gtaaaatcga ttgattttca agaaagaatc
    17281 ctttttcttg gagaagaagg tcaatacgct gtcaagtatg ccagacgtcg ctatagagaa
    17341 attcgtcaaa aatggttgaa agaaggagag taagaagatg agaatatttg ttttagaaga
    17401 tgatttttcc caacagacta gaattgaaac gacgattgag aaactcttga aggaacatca
    17461 tatcactctg agttcttttg aagtttttgg taagccagat caattgctgg cagaggtgca
    17521 tgagaagggg gcccatcagc tattcttttt ggatattgag attcgaaatg aggagatgaa
    17581 gggtctggaa gtggctagaa agattcggga tcgggatccg tatgccctga ttgtctttgt
    17641 gacgactcac tcggagttta tgcccctgtc ctttcgctac caagtgtctg ctctggatta
    17701 tattgataag gccctttcgg cagaggagtt tgaatctcgt atcgagacag ccctcctcta
    17761 tgccaatggt caagacagta aaagtctagc ggaagattgc ttttacttta aatcaaaatt
    17821 tgctcaattc cagtatcctt ttaaagaggt ttactatctc gaaacgtcca caagacccca
    17881 tcgtgttatt ctctatacca agacagacag gctggaattt acagcgagtc tagaggaggt
    17941 tctcaagcag gagcctcgtc tcttgcagtg ccatcgctct tttctcatca atcctgccaa
    18001 tgtggtgcat ttagataaga aagaaaaact gcttttcttt cccaatggtg gaagttgtct
    18061 aatcgcgcgt tataaggtca gggaagtgtc tgaggctatt aataacttac actgagctag
    18121 gagagtttat gaatgttgct tggatattgt tgtatacact tgttactcac ggattaaaaa
    18181 ttgtcatatt ctttaaggta gatggaatta gtctcacttt tgagaggatt tttaaggcct
    18241 ttctttttaa gatactgttg gccgttgttt ttggaatgct tggctatatg gtaggaaatg
    18301 tttacctatc ttattttatg gaacccttgt acggcatagg cttatctttc ttattgttaa
    18361 gagaacttcc taaaaaactc cttctctttt atggtctctt tccaatgata ttagtgaatc
    18421 tcttttatag aggtgtttcc tattttgtgc ttccattttt gggacaaggg caagtatatg
    18481 atgactactc atttatttgg ttatgtataa taattttcaa tttcttcatt tctctagctt
    18541 ttttgaaatg gttggactat gatttcacta gcttgagaaa ggggattctc gataaagatt
    18601 ttcaaaagtc cctgactcag attaactgga taatgggggc ttactatttg gtgatacaaa
    18661 atctgtctta ctttgaatat gaacagggga ttcaatcaac gactgttcgc catctcatcc
    18721 tagtctttta cctgctcttt tttatgggga ttatcaagaa attggatacc tatttgaagg
    18781 acaaactcca tgagagactg aaccaagagc aggacttgcg ctacagagag atggagcggt
    18841 atagtcggca tatagaggag ctttataagg aagtacggag ttttcgccat gactacacca
    18901 acctcttgac tagcttacgt ctgggcattg aagaggagga tatggagcag ataaaagaga
    18961 tctacgactc ggtcttaaag gattctagtg aaaaattgca ggacaataaa tatgaccttg
    19021 gccgattggt gaatgttcgg gatcgtgccc tcaaaagtct cctagctgga aaatttataa
    19081 aagctagaga taagaacatt gtctttaatg tcgaagttcc agaggagatt caggtagagg
    19141 gggtgagtct acttgatttt ctaaccgttg tgtctatcct ttgtgacaac gctattgaag
    19201 ctagtgtaga agcctgtcaa cctcatgttt caattgcctt ttttaaaaat ggagcacagg
    19261 agacctttat cattgaaaat tccatcaaag aagagggaat cgatatttct gaaatcttct
    19321 cctttggagc aagttctaaa ggggaggaga gaggagttgg tctctatacc gttatgaaaa
    19381 ttgtggaaag tcatcccaat accagtctca ataccacctg ccaagatcac gtttttcgtc
    19441 aggtacttac tgtaatacat atagaatgaa gaaaaaataa gaccgagagt gcgttctctt
    19501 ggtcttgttt ttttatagtt gtattggctg ataaaatcct tttgtatttt tttgttcaat
    19561 tatattaaat ctatataaga aatcttccca ccatcctcca cctataattt ggttgagttc
    19621 ggtagttgtt agttcttgaa atgaagttag gttttgtttc ttatccatgt tatgattctc
    19681 ctttttgata agataataaa tagttataga gtgttatctg aaaattaatc agaatgggtt
    19741 aaaattttat ctttgaaata atcaaaatat gttttctttg tagttacact agtgacgcga
    19801 ccttgtaagc catattggat gagtttacta tcctcgttag atagttttgc aagagcggtt
    19861 aatttaaaga gattgccttg ctctgttctg gtaggagttt gatcaatact ctgaagttgg
    19921 ccgatgatgg tggtgccgtg atttccaatc ttctccagtt ttaatcttac agtttgtcct
    19981 ttatctagta aggggagata gtcagatgat acgtagtaag tgattagtac ttctcttgta
    20041 tctgtgatga cagggaatat ttgagcaatt tctgtaccag ttggaattct atttttacct
    20101 tcaaattcgc tgttcagatg aacgatacct ttacttggag cggttaaggt attattttct
    20161 aagcgctgtg tcgcttgatc tagttgtact tttaattctg ttaattgatt ctccacggtt
    20221 agttgttgct gtgaggctgt ttgtaaaaac tgagtgcgga gtacttcaat tttggttgct
    20281 aaactattat cataagttgc tacacttccg ataccagctt gctgaatttt gaggcttgcg
    20341 atagatgatt caagacttgc aatactttga ttaatttgag ataaaaatgg ctcctctgcc
    20401 gttccttgtg tttgtccttg agaggctata agataacgat ttaaaattga ctggtgcgga
    20461 ttgccagttg gtaagcgtgc tctgttattt atgatagcat ctctcaactc ttgatattct
    20521 ccaatttgtt gttgaacttt tgtaatttct tgttcgatgg ctgatgaact gctattggta
    20581 agattagctt gatttgaaac ttcagtgtta gtctttgaga tacccagttc aatatcatgg
    20641 gattgtttag taaaattcat aaaggtatta tggtagccaa actcatcctc gctagaaaaa
    20701 agatcagtcg ctttttctaa gctttgtttc aagattccaa gtccttcttt ttgcttctca
    20761 agtctttgta attgagttgc taaggcagtt ttctgacttt cttccattgt ttcagagtat
    20821 ttgatgagta agtcaccttt ctcaactact tgatttgcca ctaaatgatt agcaaggata
    20881 ggattatcac tggtcgactg aatagatgca atgacacttg taggggtgat ttctccttgg
    20941 gaagtaacag taatttcttt tgtggcaaca agggagaaaa tcaagatgaa agtaaatagt
    21001 aatgaaagag gtataatgaa cactgtcgca tagttatggt aacgtctctg ataaaactcg
    21061 acacttctaa aaagattagg attcatgaca ttctccttat ttattgaata gatgatggta
    21121 gaagccttgc gcctgcatta actcttggtg actaccaact tcaatgattt tcccctggtc
    21181 aagaacaatg acacggttag ttcgttcggc tatactgaga cgatgggcta caaagagaat
    21241 ggttttatca gttagggata taagattatc tataaccttt ttctcagtca agacatcaag
    21301 accgctagta gcttcatcta gtattaaaac aggagcttta gttaaaagag cacgagcgag
    21361 agcgattcgt tgcttttgtc ctcctgatag accagctcca tcagagagct gagtttgata
    21421 gcccataggc attctttcaa tgtcttgacg gatttcagct aattcacaag ctcttagaat
    21481 atcttcttgg ctaatcatat gattaccgcc caaggttaaa ttttccaaga tagagccatt
    21541 aaagatatag gcttgttggg gtaggtaatt aatatgacgg cgcaagactt ttttatcaat
    21601 gtttttaata tcctgataat tgatggaaat atgccctttg tagggttcaa agaaattgac
    21661 aatcattttg gctaaagttg ttttaccaga accactaact ccaactaggc taaccttatc
    21721 tccttgttta atcgtgagat taatatctgt taaggtatct cgtccaaaac catacttata
    21781 agaaaggtca tcaaattcaa tatcgcccat caaaaaatgt gaatgaacag ggttttcttg
    21841 aacttgaaat tcagattcga ctagatagac ttcgttcaaa cggttattag cgaccttcgc
    21901 agattggagt ttggtttgga ggttgataat attttccata ggagttgtaa agtaagaaaa
    21961 aagtgtgtta aaggtaatca gctgaccgat agaaatttta ctcgacatga ctaattgagc
    22021 gccaaaccat aggataagga tattcagaac taattttgtt ccctgcttta aactcgtttg
    22081 taaaatagaa tatttactga gcttaaagga tttttccaaa taatctacaa attcgctgtc
    22141 tatattttga tagcgatttt cttcactcgt gagcgacttt atagtttcaa tcccgttgat
    22201 atcttcgata atggcagagc taaccataga attactttgc atgacatcat ggttcatttt
    22261 ttcgaaaggt ttcataaaag aaaagatgat gaacatgtat ataggaatgg aaagaagaga
    22321 aaggaggaag agattagggt tttgtgccag taagacgccc cctacaagaa tcagaataga
    22381 aacatccaga aaaagagaaa gaatggtaga agccaaggca tctataatag agttagcatc
    22441 tgtgaaccgt gaaatgattt ctcctgtacg acgtgtcgca aagaaagaca tgggaagttc
    22501 aaaaatatgg cgaatatagg ataaaatcac atcaatgctt aatctctggc tcagaacggt
    22561 taggagataa tctctggaaa agctcatgac ttgttggagg atataggtga taaccagacc
    22621 aactgagatg attcctaaag ttgatttcat ctgatttggg atgtattcat ccaagattcc
    22681 ttggagataa taagaaccac cgatattgat aatagtgacc aataagcttg agagaacaat
    22741 gtaagcaatg agagattttt gtttgaaaat cagaggaagg aagcttagta gaccattctt
    22801 tttatcttta tggggttgat agctgggttt gggagctaga aaaatagcta ctccagtcca
    22861 ttcagagaaa aagcgttctt ttgacatttt agtgattttt acagaagggt caggatcacc
    22921 aataatcaga tagtctttct ttgtttgata gacaacatag taatgttgga gttttccttc
    22981 tttgttaacg tgaacgataa atggataggg gacatcactc atgtcaaaga gagttttatc
    23041 tgcttgaaca ggccttgttt caaagcccat ttcatcagcg gcttttacaa tgccaagagc
    23101 agtcgtccct tctttattgg tctttgcaag ttctctcaag tgagctagag aaaaatctga
    23161 accatagaat ttagcaatcg aggctaaggc agcaacaccg cagtctctcg catctatttg
    23221 aggaacaaat gtacgtttat aagaagtcat tggcaattcc tttcatatag tggatagctc
    23281 tattatgaca taaaaacaat ttcgtgcttt ataaaatctt gaatagtcat tgaaatttcc
    23341 tgaatggtaa aaaagtgatt agaaattatc ttttaaaaaa aatttagagg tagttttaaa
    23401 taaaaaagct aatttaagac gtttcgatga caattcaaga tttggatgaa aaactttaac
    23461 aaatagtgct atactaaact tgtcaaagtt gcaacaagac aaaaattaaa aataaaaaag
    23521 gagtatttgt tatgaataca aaaatgatgg aacaatttca tgaaatggat atagctatgt
    23581 tatctagtat tgaaggaggc aagaataatt ggcaaactaa tgttttcgaa ggtggtagtg
    23641 ctgcttttgg tggctggggg ttagggacag ctatttgtgc tgcgagtggt gttggagcgc
    23701 catttatggg agcatgtgga tacataggag ctaaatttgg tgtggctctt tgggcaggag
    23761 taacaggggc aacgggtgga ttttaacaaa aggagacttg tatgaatact tattgtaata
    23821 taaatgaaac aatgctgagt gaggtttacg gaggtaattc cggaggagca gctgtagttg
    23881 ctgctttagg ttgtgcagcg ggtggagtga aatatgggaa aattctagga ccatggggcg
    23941 ctgcaatagg aggaattgga ggagcagtgg tttgtggata tttagcctat accgctacat
    24001 cataaaaatg aaagctaaat tttatgaagg ctatattctt tattattctc ttcgcttttc
    24061 aaacctatct tatttatcta tccataagta tctctgataa aaaacaaaag actattgaat
    24121 taacatcatt gaattgtttt gtcatattat ttctaatcta tgataaatta atttttctct
    24181 ttattgccta tgtttttttg ataattttta tattaaactt gttccgtaac tgaaaagtga
    24241 catactatat gaatacaaaa atgatgtcac aattttctgt tatggataat gaaatgcttg
    24301 cttgcgttga aggtggagat attgattggg gaagaaaaat tagttgtgca gcaggggttg
    24361 catatggcgc aattgatggg tgtgcaacaa cggtttgaca tttctattgg gaccatttgc
    24421 tataggaata ggtgtaactg gtgctgcagg tggagctata cttggaggta tagcctatgc
    24481 agcaacatgt tggtggtaat tatggatttt aaaagtttta ttattggttt agtagttggt
    24541 atatttggtc cttatatgga tgacttaatt agaaaaatat tttcaaaatc ttccaaaaag
    24601 gatacggata gtactctcta aaaaataaat ttctcccctg tatttgctag cttaatttac
    24661 aggggagttt ctttaatatt gtttcgcctg tcaaaatgat atgtgatagc aggatgaaaa
    24721 gtctacaaac aagttggtat caatacaata taaagaggaa agtatgtttt tcatgcttgc
    24781 ttttttaatt tttacaattc aagaggtttt gatgaccatt tatgatttga gtgatccaag
    24841 gtcaaaataa gtgctatact agcagtgtaa atgttcttgc tcaactaaga taaatcgcac
    24901 atttaggagg aaatcttatg aagaaaaaaa tactgattat tttcgttctg tatctgatca
    24961 tgtccatctt tctttatccg cttagggaga gtatttggta taatctattt tataccatag
    25021 cctatatgat tgcggttatg atctattttt ctttaattaa aaagaaagaa aagaaatgaa
    25081 agattttctt gattaaaaag aatcatattc tttgtgagat tgttcctggg tcagttgcct
    25141 ttgcttgttt ctacttatct atttctattt cgtcagtttt ccaatttctt ttagtggtta
    25201 ttaacttagc ttctattttg gtcactgttt acctcactag ggaaatgcgg ataaaagagt
    25261 ttgaagatga tgatttggtt agtcctagaa ccaatcaact tatgttcatc ggcttgacag
    25321 gctttatgtc tattatttgt ttgtatagag ggatcacagc aggagaatcc taccaacaaa
    25381 taatcgctta tattggcgct attctctgct tgaccatcat gcttctgctc atttggggat
    25441 tgaagtatta taaaaagtag aggctaatgc agtttttgca atttaagatg tcttgatgac
    25501 aattcaagat ttggatgaaa aactttgaca aatagtgcta tactgaaatt gtcaaagttg
    25561 caacgggtgc tgctacatta tgttaaaaat cgctcttgat tgataagctg gatttgttag
    25621 gaaaggagta aataattatg gataagaaaa agatagtttc gactataata tgtatagtat
    25681 ttcttgtagt gtcagtagat aattttttta gagacttaac cccgttattg ttcattttaa
    25741 atattattgg cctaacttgt tttttagtgt taacatacac taacataaaa gagatactct
    25801 taaatataag taaatgatga tttggttagt cctagaacca atcaacttat gtttatcggc
    25861 ttggtagatt ttatgtttgt catttgtttg tatagaggta tttcagaaac agaattctat
    25921 caacaactaa tcgcttatat aggtgtattc tctgcttgtt tatcacgctt ctgctcatgt
    25981 ggggcttgaa gcattataaa aagtagcttt ctatcctttt ctgacaagat atttgagtag
    26041 aaggctctat taaattgtta gtgaaataaa tgaatggagg tat
//
LOCUS       DAVKJJ010000029        25814 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000029 DAVKJJ010000000
VERSION     DAVKJJ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25814
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.029"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            343..1386
                     /locus_tag="VW144_001237"
     CDS             343..1386
                     /locus_tag="VW144_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEU9600689.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEHFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            1553..3640
                     /gene="topA"
                     /locus_tag="VW144_001238"
     CDS             1553..3640
                     /gene="topA"
                     /locus_tag="VW144_001238"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU9600690.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVYKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3746..4105
                     /locus_tag="VW144_001239"
     CDS             3746..4105
                     /locus_tag="VW144_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEU9600691.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4198..4830
                     /locus_tag="VW144_001240"
     CDS             4198..4830
                     /locus_tag="VW144_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEU9600692.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGTLTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            4874..5530
                     /locus_tag="VW144_001241"
     CDS             4874..5530
                     /locus_tag="VW144_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU9600693.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESIRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5549..6181
                     /locus_tag="VW144_001242"
     CDS             5549..6181
                     /locus_tag="VW144_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU9600694.1"
                     /translation="MNFQLAKYSLLKKFSENIGFTTPEECGAIFKYLIENVKTDRQII
                     YSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            6209..6643
                     /locus_tag="VW144_001243"
     CDS             6209..6643
                     /locus_tag="VW144_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEU9600695.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQIHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTINVVTDGIDAQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIAYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            6655..7692
                     /gene="leuB"
                     /locus_tag="VW144_001244"
     CDS             6655..7692
                     /gene="leuB"
                     /locus_tag="VW144_001244"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEU9600696.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADAILLAAIGSPQYDGAAVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
                     VERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLGVMPSASHSENGPSLYEPIHSSA
                     PDIAGQGIANPISMILSVSMMLRDSFGRYEDAERIKRAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            7689..7958
                     /locus_tag="VW144_001245"
     CDS             7689..7958
                     /locus_tag="VW144_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEU9600697.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKSKARRRVWRIPEKILLIL
                     AFTFGGFGAWLAGIIFHHKTRKWYFKIVWFLGMVTTLVALYFIWR"
     gene            7962..8321
                     /gene="leuD"
                     /locus_tag="VW144_001246"
     CDS             7962..8321
                     /gene="leuD"
                     /locus_tag="VW144_001246"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU9600698.1"
                     /translation="MAGSSREYAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8540..8683
                     /locus_tag="VW144_001247"
     CDS             8540..8683
                     /locus_tag="VW144_001247"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600699.1"
                     /translation="MTSDKAGLERKFAAKERKRNKPGVVLCGSMDELCALAQLNPEIE
                     AFY"
     gene            8944..9105
                     /locus_tag="VW144_001248"
     CDS             8944..9105
                     /locus_tag="VW144_001248"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600700.1"
                     /translation="MVDKDGKLIPEQGGARSTSPAPVVIRKGLDIDKIMMHLSDTFNS
                     WDYRQVEYY"
     gene            9269..11200
                     /locus_tag="VW144_001249"
     CDS             9269..11200
                     /locus_tag="VW144_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEU9600701.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFSHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYISPEEVETDKKFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQ
                     KDQAVADDNEGDENDDADY"
     gene            11187..12503
                     /locus_tag="VW144_001250"
     CDS             11187..12503
                     /locus_tag="VW144_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEU9600702.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLYINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSNVKGV
                     IVVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKITRRNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEIGNL
                     AGYGALLKKWSIQIPQKSSSYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            12678..13664
                     /locus_tag="VW144_001251"
     CDS             12678..13664
                     /locus_tag="VW144_001251"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEU9600703.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVSQLKADAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            13728..13799
                     /locus_tag="VW144_001252"
     tRNA            13728..13799
                     /locus_tag="VW144_001252"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13761..13763,aa:Arg,seq:ccg)
     gene            13982..14680
                     /locus_tag="VW144_001253"
     CDS             13982..14680
                     /locus_tag="VW144_001253"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU9600704.1"
                     /translation="MKELQTVLKNHFEIEFADKKLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            14671..18210
                     /gene="smc"
                     /locus_tag="VW144_001254"
     CDS             14671..18210
                     /gene="smc"
                     /locus_tag="VW144_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEU9600705.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKYDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLATAKEKASQQKDELETAKVQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDSLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVYYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRVGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHAREAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAADEASLGSEEAALKTLQDQMAALTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQESLATIASEKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEEVAQARLLKTELQGQKRYEVADIERLGKELDNLNIEQEEIQRMLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQNQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNLEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            18207..19001
                     /locus_tag="VW144_001255"
     CDS             18207..19001
                     /locus_tag="VW144_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9600706.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFTVAS
                     GRGFLSLEKLFADVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNEHVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            19001..19819
                     /locus_tag="VW144_001256"
     CDS             19001..19819
                     /locus_tag="VW144_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9600707.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            19823..21100
                     /gene="ftsY"
                     /locus_tag="VW144_001257"
     CDS             19823..21100
                     /gene="ftsY"
                     /locus_tag="VW144_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU9600708.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDLEPVVEVSQKEVEEFPHSEEGNTEFLETIE
                     ENNSEVLEPERPQAEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEEL
                     LIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQ
                     DNLTVMLFVGVNGVGKTTSIGKLAHCYKQAGKKVMLVAADTFRAGAVAQLAEWGRRVD
                     VPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKR
                     VVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREEL
                     NIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(21146..22633)
                     /locus_tag="VW144_001258"
     CDS             complement(21146..22633)
                     /locus_tag="VW144_001258"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU9600709.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATANKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVCASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDITYRQDGRLE"
     gene            complement(22782..23522)
                     /locus_tag="VW144_001259"
     CDS             complement(22782..23522)
                     /locus_tag="VW144_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9600710.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEKLGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(23522..25687)
                     /locus_tag="VW144_001260"
     CDS             complement(23522..25687)
                     /locus_tag="VW144_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9600711.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
BASE COUNT         8044 a         4547 c         5711 g         7512 t
ORIGIN      
        1 gtagagatta caaggcttgt ttaagaaaga attcaaagac cttgacaaat aaaaataaaa
       61 tggttattat aaaaaatggt ctgaaataga tgatgatact tttcgaaaat ctttttacgt
      121 cagctcagct ttgccttgct gtgttttgag caagctacgg ttagcttccg agtttgattt
      181 tcatttacta gaaatgaaac tgatgagaga tatcagtaga catttgagtc aggatattat
      241 ggaaaatgat aaaaagagct cgtgagattg gcatatcaga ctactaaagt attgagtttg
      301 ttaggatttt agcgactagt tagctgggaa aggaagatat ttgtgacaaa taataaactg
      361 tattcgttga tagaatttag aaataaaata tatgaagaat tagaactttc cagaagtgat
      421 ttagcgattt tactatgtgc catgcttatc gcctctatcg gattaaatat ggattcgact
      481 cccgtgatta ttggagccat gttaatctct cctttgatga cacctattct gggagtgggg
      541 ctctctctag ctatatttga ttttaaattg ttaagaaaat cttttaaaat attagctatt
      601 caaattcttg ccagtttaat agcttcaaca ctttattttt atctttctcc catttcgtat
      661 gctagttcgg aaattgttgc tagaacctct ccgactattt gggatgttct cattgctttt
      721 gtaggaggga tagcaggtat tattggtgct aggaaaaaag agaccaataa tattgttcct
      781 ggtgttgcta ttgcaaccgc cttgatgcct cctctttgta cagtaggtta tgctattgct
      841 tctgctaatc taaaatttat cataggctcc tcttacctat tcctcatcaa ttgtagcttt
      901 attgtcattg cgacttatat aggtgttagg ttgatgatgg ttaagaaaca ttattttaaa
      961 gataatgaag aagactctaa aatgcgtagg attttgcttc tagttgctgt tttgctgatg
     1021 attccgagtt tcatctctgc aacgacttta gtgagagaaa cgttgaaaaa agagtccctt
     1081 aagaaattta tatcagagca gtttcagggg tataatattt tgaaaaaaac ctattctaaa
     1141 aagactcata ccctaaagct aaccatttca ggaaattatt tgacagaaga agaactcgat
     1201 atgatttcaa gtaagagagg tgactatggt ttaagtgatg tttctgttca agtttcacaa
     1261 ttgtctgatt cagaacaact tagtaaggaa gagctggtgg agcatttctt ccagtatatc
     1321 aaggataagg aagcaaaaga aaaggagaaa gccaataaat ttgatacaga gtctgaggag
     1381 caataatttc ttgagaatag ctggtttctc tcgtgagtct tctatgtata tcaaaggaag
     1441 actgaggttt taagtataaa acttttcttc tattatagta gctaagtctt gacagggttc
     1501 aaaaaatggt ttacacttta taaagtttat tactttgaaa aggtgtgata ctgtggctac
     1561 ggcaacaaaa aagaaaaaat caacagttaa aaaaaatcta gtcatcgtgg agtcgcctgc
     1621 taaggctaag acgattgaaa aatatctagg cagaaactac aaggttttag ccagtgtcgg
     1681 gcatatccgt gatttgaaga aatccagtat gtccgtcgat attgaaaata attatgaacc
     1741 gcaatatatt aatatccgag gaaaaggccc tcttatcaat gacttgaaaa aagaagctaa
     1801 aaaagctaat aaagtttttc tcgcgagtga cccggaccgt gaaggagaag cgatttcttg
     1861 gcatttggcc catattctca acttggatga aaatgatgcc aaccgtgtgg tcttcaatga
     1921 aatcaccaag gatgcagtca aaaatgcttt taaagaacct cgtaagatcg atatggactt
     1981 ggtcgatgcc caacaagctc gtcggatctt ggatcgcttg gtagggtatt cgatttcgcc
     2041 tattttgtgg aagaaggtca agaagggctt gtcagcaggt cgcgttcagt ccattgccct
     2101 taaactcatc attgaccgtg aaaatgaaat caatgccttc cagccagaag aatactggac
     2161 agttgatgct gtctttaaaa agggaaccaa acaatttcat gcttccttct atggagtaga
     2221 tggtaaaaag atgaaactga ccagcaatga cgaagtcaag gaagtcttgt ctcgtctgac
     2281 tagtaaagac ttttcagtag atcaggtgta taagaaagag cgcaagcgca atgctccttt
     2341 accctatacc acttcatcta tgcagatgga tgctgccaat aaaatcaatt tccgtactcg
     2401 aaaaaccatg atggttgccc aacagctcta tgaaggaatt aatatcggtt ctggtgttca
     2461 aggtttgatt acctatatgc gtaccgattc gactcgtatc agtcctgtag cgcaaaatga
     2521 ggcggcaagc ttcattacgg atcgttttgg tagtaagtat tctaagcacg gtagcaaggt
     2581 caaaaacgca tcaggtgctc aggatgccca tgaggctatt cgtccgtcaa gtgtctttaa
     2641 tacaccagaa agcatcgcta agtatctgga caaggatcag ctcaagctat atacccttat
     2701 ctggaatcgt tttgtggcta gccagatgac agcggccgtt tttgatacca tggctgttaa
     2761 attgtctcaa aaaggggttc aatttgctgc caatggtagt caggttaagt ttgatggtta
     2821 tcttgccatt tataatgatt ctgacaagaa taagatgtta ccggacatgg ttgttggaga
     2881 tgtggtcaaa caggtcaata gcaaaccaga gcaacatttc acccaaccgc ctgcccgtta
     2941 ttctgaagca acactgatta aaaccttaga ggaaaatggg gttggacgtc catcaaccta
     3001 tgcgccaacc attgaaacca ttcagaaacg ttattatgtt cgcctggcag ccaaacgttt
     3061 tgaaccgaca gagttgggag aaattgtcaa taagctcatc gttgaatatt tcccagatat
     3121 cgtaaacgtg accttcacag ctgaaatgga aggtaaactg gatgatgtcg aagttggaaa
     3181 agagcagtgg cgacgggtca ttgatgcctt ttacaaacca ttctctaaag aagttgccaa
     3241 ggctgaagaa gaaatggaaa aaatccagat taaggatgaa ccagctggat ttgactgtga
     3301 agtgtgtggc agtccaatgg tcattaaact tggtcgtttt ggtaaattct acgcttgtag
     3361 caatttccca gattgccgtc atacccaagc aatcgtgaaa gagattggtg ttgagtgtcc
     3421 aagctgtcat cagggacaaa ttattgagcg aaaaaccaag cgtaatcgcc ttttctatgg
     3481 ttgcaatcgc tatccagaat gtgaatttac ctcttgggac aagcctgttg gtcgtgactg
     3541 tccaaaatgt ggcaacttcc tcatggagaa aaaagtccgt ggtggtggca agcaggttgt
     3601 ttgtagcaaa ggcgactacg aggaagaaaa gattaaataa gaggaggggc ccgaaagtaa
     3661 atttcaggct ccttttggtt agaacttgac aaaatacatc attttatgcg aaactagaag
     3721 aagattattt taactaggag aagttatgcg tcttatctat ctgataattg gttttttatc
     3781 actggccttg gctattgttg gggttgtttt acccttgttg ccgacaacgc ctttcctttt
     3841 gttgtctatt gcttgtttct ccagaagttc caagcgtttc gaagactggc tttatcatac
     3901 caagctctat caaacatatg tagctgattt tcgcgagact aagtctatta cgcgtgaacg
     3961 aaagaaaaaa atcatcgtct ctatctacgt cttgatggga atttctattt attttgcacc
     4021 tcttttacca gtcaaaatcg gtctgggtgc tttgaccatc tttatcactt attatctttt
     4081 caaggtcatt ccagacaaag aatagttaaa acagtagtta tttgccttga taaaattgaa
     4141 agcatattca taataatatg atataataaa tttgaagtaa tattcaagga gaatcaaatg
     4201 atttacgaat tttgtgctga aaatgtgact ttacttgaaa aagcgatgca ggctggagct
     4261 cgtcggattg aactctgtga taatctagca gttggtggga caacacccag ctatggagtg
     4321 actaaggcag cggttgaact ggcagctaac tacgatacaa ccatcatgac catgattcgg
     4381 ccacgtggtg gtgactttgt ctataatgac ctagaaattg ctatcatgct agaagacatt
     4441 cgtttgactg ctcaggctgg aagtcaaggg gttgtatttg gaactttaac tgctgataaa
     4501 aagttggata agcctaatct ggaaaagtta attgctgcat caaaaggaat ggaaattgtc
     4561 tttcacatgg cctttgatga actaagtgat gaagatcaac cggaagctat tgactggctc
     4621 agtcaagccg gtgtcactcg tatcctaact cgtgctggtg tgtctggcga ctccttagaa
     4681 aaacgttttg ttcactatca cagaattttg gagtacgcta aaggtaaaat tgaaattcta
     4741 ccaggtgggg ggattgacct tgaaaaccgt caaaccttta tcgaccaggt gggggtaaca
     4801 caattgcatg gtactaaggt tgttttttaa aaaatagaaa ggaactgcta gccttgggta
     4861 gcagttttca cttatgtttg aaatttttaa atcctatcaa tttaatcaag aaaaggctca
     4921 tgattatggt tttatagaaa atagcgaagt ctggacatat agttgccaga ttttgcaagg
     4981 tgactttgtc atgactgtgt ccatcactgc tgataatgtg aactttcaag tctttgacca
     5041 agagactggt gacctctatc ctcacgttca tatggaaagc ataaggggaa gttttgtcgg
     5101 aaatgtccgt gaggcttgtc tggagattct ttaccagatt cggaaggctt gttttgatgt
     5161 gcaagatttt atctgtcatc agactaagcg tatcatgact caagttcagg aaaagtatgg
     5221 aaaccagttg gagtatctgt gggaaaaatc gcctgataca gctgtattgc gccatgaagg
     5281 caatcaaaag tggtatgccg tcttgatgaa aatctcttgg aataagctgg aaaagggcag
     5341 agaaggacaa gtggaagcag tcaacctcaa gcatgaccaa gtagctaatt tgctttcaca
     5401 aaaggggatt tatccagcct tccatatgag caagcgctac tggattagtg tgtcccttga
     5461 tgatacttta tcagatgaag aagtactgga attgatagaa aaaagttgga acttaacctc
     5521 taaaaaatga aatattttaa taattttcat gaactttcaa ttagctaaat attctttact
     5581 gaagaaattt tcagaaaata taggatttac cacaccagag gaatgtggtg ccatcttcaa
     5641 atacctgatt gagaatgtca agacggatcg tcagatcatc tattcgcctc actgtcatga
     5701 tgacctcgga atggcagtgg caaatagcct tgctgctgtc aagaatggtg caggacgtgt
     5761 tgaagggact atcaatggta ttagggagcg agctgaaaat gctgctttgg aagaaattgc
     5821 agtggctctc aatattcgcc aagattacta ccaagtagaa accagtattg tcctaaatga
     5881 gaccatcaat acgtcagaaa tggtttctcg cttctctggt attccagttc ctaaaaacaa
     5941 agccgtcgtt ggtggcaata ccttctccca cgaatctggt attcaccaag atggagtcct
     6001 taaaaatcct ctcacttatg agatcatcac acctgaattg gttggtgtta agattccgct
     6061 tggaaaatta tctggtcgcc atgcttttgt tgagaaactg agagaattgg ccctagattt
     6121 tacagaagag gatatcaaac cactctttgc taagttcaag gcactggccg ataagaagta
     6181 agaaatcaca gatgcagata ctcgagcttt ggtagctgga accatggttg aaaatccaga
     6241 aggcttccac tttgatgatt tacaacttca aattcatgca gataatgaca ttgaagcgct
     6301 cgttagccta gccaatatgg atggtgagaa agtcgaattt aatgcgacag ggcaaggttc
     6361 tgttgaagca atctttaacg ctatcgataa gttctttaac caatctgttc gtttggtgtc
     6421 ctacactatc aatgtggtaa cagatggaat cgatgcccag gatcgggttt tggtcactgt
     6481 tgaaaacaga gatacagaaa ccatctttaa tgcagcaggg cttgattttg atgtgttgaa
     6541 ggcttctgct attgcctata taaacgctaa tacctttgtt caaaaagaga atgcaggtga
     6601 gatgggacgc agtgtttctt accacgatat gcctagtgtg taaaggagaa ggctatggca
     6661 aagaaaatag tagctctagt aggagacgga attggcccag aaatcatgga ggctggttta
     6721 gaagttctgg aggctctagc tgaaaaaaca ggttttgact atgagattga cagacgaccg
     6781 ttcggaggtg cagatattga tgcagcagga cctcccttac ctgatgaaac ccttaaggca
     6841 agtagggaag cagatgctat cctactagca gctatcggta gtcctcagta tgatggagca
     6901 gcggttcgcc ctgaacaagg cctgatggct ctccgtaagg aactcaatct ttacgctaat
     6961 attcgtcctg taaaaatctt tgacagtctc aagtatttgt caccactcaa accggaacga
     7021 atttctggtg tagactttgt cgtggtgcgt gaattgacag gcgagattta ctttggagat
     7081 catattcttg aagagcgcaa agcgcgtgat atcaacgact atagctatga ggaagtggag
     7141 cggattattc gcaaagcctt tgaaattgca agaaatcgga gaaaaatcgt tactagtatc
     7201 gataagcaaa atgttctagc gacctcaaaa ctctggcgga aagtagctga ggaagtcgca
     7261 caggatttcc cagatgtaac cttggaacat cagctggtag actcagctgc tatgcttatg
     7321 attaccaatc ctgctaagtt tgatgttatt gtaacggaga atctttttgg agatatttta
     7381 tctgatgaat caagcgtctt atctggtaca cttggggtta tgccatcagc cagtcattct
     7441 gaaaatggac caagtctcta tgaacctatt cacagttcag cacctgatat tgcaggtcaa
     7501 ggaattgcca atcctatttc catgatttta tcagtttcca tgatgttgag agatagtttc
     7561 ggacgttatg aggatgcaga gcgtatcaaa cgtgctgttg agacaagtct ggcggcagga
     7621 attttaacga gagatatagg aggtcaggct tcaacaaagg aaatgacgga agctattatt
     7681 gcaaggttat gaagttagac gaaaaaatta ctctagtcct tttgatttgg aatgtcatca
     7741 ttttcttgat ttatggtatt gacaaatcta aggcaaggag aagagtttgg cgcatccctg
     7801 agaaaatctt acttatttta gcctttactt ttggtggttt tggtgcctgg ctagcaggaa
     7861 tcatctttca ccacaagact cgaaaatggt actttaaaat agtttggttt cttgggatgg
     7921 tgaccacact agtagcctta tattttattt ggaggtaatg gatggcaggg tcttcgaggg
     7981 aatacgctgc ttgggctcta gcggactatg gttttaaggt cgtgattgca ggatctttcg
     8041 gtgacattca ttacaataat gaactcaata atggcatgtt gccaatcgtt cagcctagag
     8101 aggttagaga gaaactagcc cagctaaaac caaccgacca ggtaactgtg gacttggaac
     8161 aacaaaaaat catctcacca gttgaagaat tcaccttcga gatagatagc gagtggaaac
     8221 ataaactcct aaatagtttg gatgatatcg gtattacctt gcagtatgaa gagttgattg
     8281 ctgcttatga aaaacaacga ccagcctact ggcaggatta gaaaaaatag aaaaggagat
     8341 atagtaaact gaaataagat gtaaacaaat gaattggagc ttaacatcca tttccagcaa
     8401 ttttttagaa actacagtgg actattctgg attcaacaca ttataaaatt atgacaaaac
     8461 acattcacaa gaaggctacg acattttaaa aggtgagggc ggatgtatcg tttgccctac
     8521 taaagttggt tacattatca tgaccagtga caaggcagga cttgagcgta agttcgcagc
     8581 caaagaacgt aagcgtaaca aaccaggtgt tgttctctgc ggtagcatgg atgaactttg
     8641 cgctttagcg caactcaacc cagaaattga agcattctac taaaaacatt gggatgaaga
     8701 tattcttctt ggttgtatcc ttccttggaa accagaagcc tttgaaaaac tcaaagcata
     8761 cggggatggc cgtgaagaac ttattactga tgtacgtggt actagctgtt ttgttatcaa
     8821 gtttggaaaa gcaggtgaac aattggctgc caagctttgg gaagaaggta aaatggtcta
     8881 cgcctcatct gcttcaatga caaaacgatt gaaactcgct atgagcaagg tgtaatggtg
     8941 tctatggtcg ataaggacgg caaactcatc ccagaacaag gaggagcacg ttcaacttca
     9001 ccagctccag ttgtgatccg taaagggctt gacattgata aaatcatgat gcacctgtca
     9061 gatactttta actcatggga ctaccgtcag gttgagtatt attaggatag agaagaagtc
     9121 tagtgttatg agatattaaa gctcctaaca ctgggctttt gtttagaatt tcttttcttt
     9181 ttctatagga tatggtattc tatgtagaaa atatatgtta ataagtaatg ccaatattta
     9241 aacatcatta gtaaaaggag ttagattgat gaataaaaga aaagttagtt tagaagattt
     9301 ttataaatgg tatagtctaa ataaagaaga gttattaaat aaggcaactg ttggtgaaaa
     9361 gtttaatgat aaattaaaag aagagtttct ccaggaatgg cctttggata ggattttaac
     9421 aatgtcaatc gatgaatatg taataggaaa gggacagcaa aataagtctt tatgctacgc
     9481 tcttgagaag ggaaaataca aaaatctatt tcttggaatt tctggtggct cagcttcaaa
     9541 atttggtatt tattggaata aaaaaacaaa caaatataaa gatcaagcta ataatgagat
     9601 ttcagagttg gatcagcgat tttcaaaatt aaaatcagat ttgtatgaaa ttatcaaaga
     9661 aggtattcgt tttaactttg aaaatcctat ttttgatatg aaaagatcaa caaatgaatt
     9721 tattggtcgt tctgctatgg tgacaaaatt actttgtatc tatactgagg gagatccttt
     9781 ctttggtgta aatattaata gtcagaaaga attttggaac cactttgttt ctcagacaaa
     9841 tcaaggtgga ccttatctgc aaaatcataa aataattgaa ctggtgtcca aaacttatcc
     9901 tgagttggag ccatcgaaat taggaactat gctttttgag tattctaagc tttttatgga
     9961 aaataaggaa gacaatagta caatggattc atcaaacaat tttagtcatc aattaactca
    10021 atctctatta aagtctccaa accttatcct tcgtggtgct cctggcacag gaaagactta
    10081 tcttgctaaa gaaattgcca aagaattaac ggatggtaac gaagaccaaa tcggatttgt
    10141 acaatttcac ccatcctatg attatacgga ttttgtagaa ggtttaagac cagtatcaaa
    10201 tggggatgga gctattgagt ttaggctaca ggacggtatt tttaaagatt tttgtcagaa
    10261 agcaaaagaa acccaattga ttggaggaca agataatttt gatgaggctt gggattctta
    10321 cttagaatat ataaatgttg ctgaagaaaa agaatatata acaaaaacat cttacttatc
    10381 tgttaatagt agacaaaatt tgtcagtaaa ttatgatagt ggtgttccag gatggtcact
    10441 acctagcaaa tatgtttacg agttgtataa agataaaaat tataataagc aagaatacta
    10501 caaaagtggt ggaaaaactg tcctagaaac attgagaaag agatttggtt tgaaagacta
    10561 tatttctcca gaagaggttg aaacagacaa gaaatttgtt ttcatcatcg atgagatcaa
    10621 tcgtggggag atttctaaga tttttggcga actctttttt tctatcgacc ctggctatcg
    10681 tggtgaaaaa ggaagtgttt ctacccaata tgcaaatcta cacgaaactg atgaaaagtt
    10741 ctatatcccc gaaaatgttt acatcatcgg aactatgaat gatattgatc gttcagtgga
    10801 tacctttgat tttgctatgc gtcgtcgttt tcgttttgtt gaagttactg ccgagggtca
    10861 agttggcatg ttggataaag agctgaatat ccatgcagaa gaagcaaaaa ttcgtctaag
    10921 aaacttgaac gctgctatcg aaaatgttca ggaattaaac agtcattatc atattggacc
    10981 aagttatttt cttaagttga aggatgtaga ttttgactat gaattactct ggtctgatta
    11041 tattaagcct ctcctagaag actacttgcg aggttcttat gatgaggttg aaactttgga
    11101 aactttgaaa aaagcatttg aactgacaaa taatgagcaa aaagatcagg cagtagctga
    11161 tgacaatgaa ggtgatgaaa acgatgatgc ggattactga taatcaacac aagattatta
    11221 aagaaaaatt tgttgaagaa tatcctaaac taagcaatct tcttttagac agaaccttgg
    11281 aaagtctatc ccaagatgaa cgtattttca tttttccaaa tgatttgact catactcctg
    11341 atttggataa ggatcaaaag atttttgaaa cagtcaatca gaaaatcaag acagggaacg
    11401 tgattggttt tcttggatat ggtcaggaaa gattaacgat ttcctcacga ttttctgatg
    11461 agagtaatga ccattttttg cattatctct taaacaaggt tctttatatc aatctcacta
    11521 gtttagatgt tgctttgtct cgtgaagaga ggctttatca acttttgatg tatctcttcc
    11581 ccaagtatct acaagctgct attcgaaaag gtctttataa ggaatatcat cgattttctc
    11641 ataacgacag taatgttaag ggagtgattg ttgtaagaaa ccatctcaag aaaaatcttc
    11701 ctttcacggg aaatattgcc tacacaacga gagagttcac ctatgataat cccctcatgc
    11761 agttggtccg tcacactatt gaatacatta agaatcagaa aagcattggt caaggggtac
    11821 tagataatct ctcaactagt cgtgaaaacg tatctgaaat cgtgcgtgta acgccctctt
    11881 ataaactagc tgatcgtgct aagattactc ggagaaatca atctaaacct atacgtcatg
    11941 catactttca cgagtacaga aacttacaag aactttgtct gatgatccta aaccaagaaa
    12001 agcacggttt agggtatcaa gatcaaaaaa tctatggtat tctctttgat gttgcctggc
    12061 tttgggaaga gtatgtttac accttgttgc caaaaggttt tgtacatccc agaaataagg
    12121 ataagacgga tggaatttca gtattttctg ttgggaaacg aaaagtatat ccagattttt
    12181 atgacagaga acgaaagatt gttctagatg caaaatataa aaaactggaa ttgactgaaa
    12241 aaggaatcaa ccgtgaggac ttattccagc tgatttccta ttcttatatt ttaaaagctg
    12301 agaaggctgg acttgttttt ccgagcaagg acaaggtcat tgataatgag ataggaaacc
    12361 tagcaggtta tggagctttg ttgaagaaat ggtctatcca aattcctcag aagtcttcat
    12421 cttatagtgc attttgtaaa atgatggaaa attcagaaga aaattttaaa gcgattattg
    12481 atgaagaagt ggggagaaaa taatttcccc tctttctttt atttttaaca caagcaaaat
    12541 ccgaacaata tattttaatt cataaaataa tttgacaaaa actgtacttt ggtttataat
    12601 aaattaagga aacgtcggaa aaggcgtagt gatgcgagag cataggtagg tcattacaaa
    12661 agaaacgaga catcgatatg ttaaatgaat ttccaatttt tgattacgaa gatattcaat
    12721 tgattccaaa taaatgtgtc attaaaagcc gtgcagaagc ggatacaagt gtcactttag
    12781 gaaatcacac ctttaaacta cctgttgtgc cagcgaatat gcagacgatt ttggatgaaa
    12841 atgtagcaga gcaactggct aaaggtggtt acttctacat tatgcaccgt tttgatgagg
    12901 caggacgcat tccttttatt aaacgaatgc acgatcaagg gctcattgct tccatatctg
    12961 tcggtgttaa ggattatgag tatgattttg ttagccagct caaggctgat gctcctgagt
    13021 atatcactat tgatattgct catggtcatg cggatagcgt gatttctatg attcaacaca
    13081 tcaagaaaga attgccagat acctttgtca ttgctggaaa tgtgggaaca ccagaagctg
    13141 tgcgtgaatt ggaaaatgct ggtgcagatg ctactaaggt cggaatcggt cctggtaagg
    13201 tttgtatcac caaggttaag actggttttg gtacaggtgg ttggcagttg gctgctctac
    13261 gctggtgtgc caaggctgcg cgtaaaccga ttatcgctga tggaggaatt cgtactcacg
    13321 gtgatattgc caagtctatc cgcttcggtg ctagcatgat catgattggt tccctctttg
    13381 caggacacat tgaaagtcca gggaaaacga ttgaagtcga tggtgaacag ttcaaagaat
    13441 attatggttc agcctcacaa tatcagaaag gtgcttacaa aaatgtggaa ggcaaacgta
    13501 tcttgcttcc tgctaaaggt catctgcaag acactttaac tgagatggaa caagaccttc
    13561 aaagtgctat ttcttatgca ggtggacgtc aggttgctga ccttaaacac gttgattatg
    13621 tgatcgtgaa aaactccatc tggaatgggg atgcttccca ctaagacggg gtctttgacc
    13681 aaaaaacttg ttattagagc aaatttctgt tataataaaa caagtttcca cccttagtgt
    13741 aatggatatc acgtaagatt ccggttcttg agatgggggt tcgattccct cagggtggat
    13801 gtaaacatcc taaaaagcct ttaatagggc ttttttcttt atcctgaccc aaatttgccg
    13861 ctaaaactga gaatctctgt ctaaagaatt cccaaatgct ttccagtccc gttttagagt
    13921 gactcagggg gctttttttg atataataaa aaggactatt atcagttaga aagaagttgg
    13981 tatgaaagaa ttacaaactg tactaaagaa tcattttgaa atcgaatttg cagacaaaaa
    14041 gttactggaa actgccttta ctcatacgag ttatgccaat gagcaccgcc tcttaaaaat
    14101 ttcacacaat gaacgcttgg aatttttagg agacgctgtt ctacagttat tgatttcaga
    14161 atatctatat aaaaaatatc ctaaaaagcc tgaaggtgac ctatcaaaac tccgtgctat
    14221 gattgtccgt gaggagagtt tagctggttt tgcgcgtgat tgccagtttg accagtttat
    14281 caagttgggt aaaggggaag aaaaatctgg tggtcgcaat cgtgacacca ttcttggtga
    14341 tgcctttgaa gcctttcttg gtgcccttct tttggataag gatgtggcaa aggtcaagga
    14401 atttatctac caagttatga ttcctaaggt agaagcaggc gaatttgaga tgattacaga
    14461 ctataaaacc catctccaag agttgcttca ggtcaatggt gatgtggcta ttcgttatca
    14521 agtgatttct gaaacagggc ctgctcacga taaggtcttt gatgtagaag ttcttgttga
    14581 aggtaagagc atcggtcaag gccaaggtcg ttctaagaaa ttagcagagc aggaagctgc
    14641 caaaaatgcc gttgagaaag ggctggattc atgtatttaa aggaaatcga aattcagggg
    14701 ttcaagtctt ttgctgataa gaccaaggtc gtttttgacc aaggtgtgac ggcagttgtt
    14761 ggacccaatg gatctggaaa gtccaatatt acagaaagtc tgcgttgggc tttgggggag
    14821 tctagtgtca agagtctccg tgggggcaag atgccggatg tcatctttgc tggaacagaa
    14881 agtcgcaaac cgctcaatta tgcttctgta gttgtgactc tggataatca tgacggattt
    14941 atcaaggatg caggtcaaga aatcagggtg gaacgccata tctatcgtag tggagatagc
    15001 gaatacaaga ttgacggcaa gaaagtccgt ctgcgtgata ttcatgacct cttcttggat
    15061 actggattgg gacgagattc cttctctatt atttcccaag ggaaggttga ggagattttt
    15121 aattccaagc ctgaggaacg acgagctatt tttgaagaag ctgctggagt tttaaaatac
    15181 aagactcgca gaaaagaaac cgagagtaaa ctgcagcaaa ctcaggataa tctggaccgc
    15241 ttagaggaca ttatctacga gttggataat caaatcaagc ctcttgagaa gcaagctgag
    15301 aatgcccgta agtttttaga cttggaagga caacgtaagg ctatttattt agacgttctg
    15361 gttgctcaaa tcaaggaaaa taaggcagaa ctagagtcga cagaagaaga gttggctcag
    15421 gttcaagaac tcttgatgag ttattaccaa aagcgtgaaa aattagaaga agaaaatcaa
    15481 actcttaaaa agcaacgcca agatttacag gctgaaatgg ccaaagacca aggcagtttg
    15541 atggacttga ctagtctgat tagtgattta gaaagaaaat tagccctatc gaaactggag
    15601 tccgagcaag tggccctgaa tcaacaggag gcacaagctc gtttggctgc tttggaggat
    15661 aagagaaatt cactcagcaa agaaaagtat gataaagaaa gctctttagc tctgttagag
    15721 ggaaatctag tccaaaataa tcaaaaactc aatcgtttag aagctgaatt gctggctttc
    15781 tcagacgatc ctgatcagat gattgagctc ttacgtgaac gctttgtagc tcttttacaa
    15841 gaagaagcgg atgtctcaaa ccagttgacc cgtattgaga atgagttgga aaatagtcgt
    15901 cagctttctc aaaaacaagc agatcaacta gaaaagctga aagagcaatt agctacagct
    15961 aaagagaagg ctagtcagca aaaagacgag cttgaaactg ccaaggtgca ggttcagaaa
    16021 ttattggctg actatcaagc tattgccaag gagcaagagg agcagaaaac ttcctatcaa
    16081 gctcaacaaa gtcaactctt tgaccgtctg gatagtctca aaaacaagca ggccagagct
    16141 caaagtttgg aaaatatcct gagaaatcat agtaactttt atgcaggtgt taagagtgtt
    16201 ctccaagaaa aagatcgcct aggtgggatt attggtgcag tcagtgagca tctgaccttt
    16261 gatgtttatt atcaaactgc cctagagatt gccttagggg caagtagcca gcatatcatc
    16321 gtagaagatg aagagtcggc aaccaaagct attgatttcc tcaaacgaaa cagagtcggt
    16381 cgtgcaacct ttcttccttt gaccactatt aaggcgcgta cgatttctag tcagaaccaa
    16441 gatgctatcg ctgtaagccc aggtttcctt gggatggcag atgagttggt gacttttgat
    16501 actagactgg aagccatttt caagaacttg ctagctacga cggctatctt tgataccgta
    16561 gaacatgcgc gtgaagctgc tcgacaagtt cgttatcagg ttcgtatggt gacattggat
    16621 gggacagaat tacgcacggg tggttcctat gcgggtggtg ccaatcgcca aaataacagt
    16681 attttcatca agccagaact ggagcaatta caaaaagaaa ttgctgcaga tgaagcaagc
    16741 ttgggttcag aagaagcggc tttgaagacc ttgcaagacc agatggctgc attgacagaa
    16801 agattagaag ccatcaaatc tcaaggagag caggcacgta ttcaggagca aggcttgtcc
    16861 ctcgcttatc agcaaactag tcagcaagtt gaagaactgg aaactctttg gaaactccaa
    16921 gaagaggaaa tagatcgtct ttctgaggga gattggcaag cggataagga aaaatgtcaa
    16981 gagagccttg ctactatcgc cagtgaaaag caaaatctgg aagctgagat tgaagaaatt
    17041 aagtctaata aaaacgccat ccaagaacgc tatcaaaatt tgcaggaaga ggtggcgcaa
    17101 gctcgcttgc ttaagacaga actgcaaggg caaaaacgtt atgaagtagc tgatattgag
    17161 cgtttaggca aggaattgga caatcttaat atcgaacaag aagaaattca gcgcatgctc
    17221 caagaaaaag ttgacaatct tgagaaggtt gatacagaat tgctcagtca acaggcggaa
    17281 gaatccaaaa ctcagaaaac aaatctccaa caaggtttga ttcgcaagca gtttgagttg
    17341 gatgatatag aaggtcaact ggatgatatt gccagtcact tggatcaagc tcgccagcag
    17401 aatgaggagt ggattcgcaa gcaaacacgt gctgaagcca agaaagaaaa ggtcagcgag
    17461 cgcttgcgcc atctacaaaa tcaattaaca gaccagtacc agattagcta tactgaagca
    17521 ctagaaaagg cacatgaatt ggaaaacctc aatctggcag agcaagaggt gcaggattta
    17581 gagaaggcta ttcgctcatt gggacctgtc aacttggaag ctattgacca gtacgaagaa
    17641 gttcacaacc gtctggactt tctaaatagt cagcgagatg atattttgtc agcgaaaaat
    17701 ctgctccttg aaaccattac agagatgaat gatgaggtca aggaacgctt taaatcaacc
    17761 tttgaagcta ttcgtgagtc ctttaaagtg accttcaagc agatgtttgg cggaggtcag
    17821 gcagacttga tattgactga gggcgacctt ttaacagctg gtgtggagat ttctgttcaa
    17881 cctccaggta agaaaatcca gtcgcttaac ctcatgagtg gtggtgaaaa agctctatcg
    17941 gctcttgcct tgcttttctc cattattcgt gtcaagacca ttccttttgt catcttggat
    18001 gaggtggaag ctgcgctgga tgaagccaat gttaaacgtt ttggggatta cctcaaccgc
    18061 tttgacaagg acagccagtt tatcgttgta acccaccgta agggaaccat ggcagcggct
    18121 gattccatct atggagtgac catgcaagaa tcgggtgttt caaagattgt ttcagttaag
    18181 ttaaaagatt tagaaagtat tgaaggatga caattaaact agtagcaacg gatatggacg
    18241 gaaccttcct agatgggaat ggacgctttg atatggatcg tctcaagtct ctcttggctt
    18301 cctacaagga aaaagggatt tactttacgg tggcttcggg tcggggattt ctgtctctgg
    18361 aaaaattatt tgctgatgtt cgtgatgaca ttattttcat cgcggaaaat ggcagtttgg
    18421 tagagtatca aggtcaggac ttgtatgaag cgactatgtc tcgtgacttt tatctggcaa
    18481 cttttgaaaa gctgaaaacg tcaccttatg tagatatcaa taaactgctc ttgacgggta
    18541 agaagggttc atatgttcta gatacggttg atgagaccta tttgaaagtg agtcagcatt
    18601 ataatgaaaa tatccaaaaa gtagcgagtt tggaagatat cacagatgac attttcaaat
    18661 ttacaaccaa cttcacagaa gaaacgctag aagctggtga agcttgggtt aatgaacatg
    18721 tccctggtgt taaggccatg acaactggct ttgaatctat tgatattgtt ctggactatg
    18781 tcgataaggg tgtagctatt gttgaattag ctaaaaaact tggtatcaca atggatcagg
    18841 tcatggcttt tggagacaat cttaatgact tacatatgat gcaggttgtg ggacatcctg
    18901 tagctcctga aaatgcacga cctgaaattt tagaattagc aaagactgtg attggtcacc
    18961 ataaggaacg gtcggttata gcttatatgg agggcttata atggcagata taaaattgat
    19021 tgcattggac ttggacggga ccttgctgac tactgataaa aggctgacgg atcgtaccaa
    19081 ggaaaccttg aaagctgcgc gtgatcgtgg tatcaaggtc gtattgacaa ctggtcgtcc
    19141 cttaaaagcc atggatttct ttctccatga gttagggact gacggtcagg aagatgagta
    19201 taccattact tttaatggtg gattagttca gaaaaataca ggagaaatcc ttgataaaac
    19261 agtcttttca tatgatgatg tggcacgttt gtatgaagaa acagagaaat tatcactgcc
    19321 tcttgatgcc atctcagaag gaacagttta tcaaatccaa tcggaccaag aaagtcttta
    19381 tgccaaattc aatccagctt tgacctttgt tccagtggac tttgaagact tatctagtca
    19441 aatgacctac aacaaatgcg tgactgcctt tgctcaagaa cccttggatg cagccattca
    19501 gaagatttct ccagaattgt ttgaccaata tgaaatcttt aaatcacgtg aaatgttgct
    19561 agaatggtca ccaaagaatg ttcataaagc aacaggtttg gcaaaactaa tcagccatct
    19621 tggaatcgac caaagtcaag tgatggcttg tggtgacgag gccaatgacc tctctatgat
    19681 tgaatgggca ggtcttggtg ttgctatgca aaacgctgtt cctgaagtaa aggcagccgc
    19741 aaatgtagtg acgccgatga ccaacgatga ggaagctgtc gcctgggcta tcgaagaata
    19801 tgtgctaaag gagaactaag atatgggatt gtttgaccgt ctattcggaa aaaaagaaga
    19861 acctaaaatc gaagaagttg taaaagaagc tctggaaaat cttgatttgt ctgaagatgt
    19921 tgatcctacc ttcacagaag ttgaggaagt ttctcaggaa gaagcagagg ttgaaattgt
    19981 tgaacaagct gtgttccaag aagaggaaat ccaagacaca gttgaagaaa gtctggattt
    20041 agagccagtt gtagaagttt ctcaaaaaga agtcgaagaa tttccacact cagaagaagg
    20101 gaatactgag tttctagaga ctatagaaga aaataattct gaagttcttg aaccagaaag
    20161 gcctcaagca gaagaaaccg ttcaggaaaa atatgaccgc agtcttaaga aaactcgtac
    20221 aggtttcggt gcccgcttga atgccttctt tgctaacttc cgctctgttg acgaagaatt
    20281 tttcgaggaa ctggaagaac tgctgattat gagtgatgtt ggtgtccaag tcgcttctaa
    20341 cttaacggag gaactacgtt acgaagccaa gcttgaaaat gccaagaaac ctgatgcact
    20401 tcgtcgtgtc atcattgaga aattggttga gctttatgaa aaggatggta gctacgatga
    20461 aagcatccac ttccaagata acttgacagt tatgctcttt gttggtgtga atggtgttgg
    20521 gaaaacaact tctatcggaa aactagccca ctgctacaaa caagctggta agaaggtcat
    20581 gctggttgca gcagatacct tccgtgcggg tgcagtagct cagctagctg aatggggccg
    20641 acgagtagat gttccagtag taactggacc tgaaaaagct gatccagcca gcgtggtctt
    20701 tgatggtatg gaacgtgccg tggctgaagg tatcgatatt ctcatgattg atactgctgg
    20761 tcgtctgcaa aataaggata accttatggc tgagttggaa aagattggtc gtattatcaa
    20821 acgtgttgtg ccagaagcac cacatgaaac cttcttggca cttgatgcat caacaggtca
    20881 aaatgcccta gtacaggcca aagaattttc gaaaatcaca cctttaacgg gaattgtttt
    20941 gactaagatt gatggaactg ctcgaggagg tgtggttcta gccattcgtg aagaactcaa
    21001 tattcctgta aaattgattg gttttggtga aaaaatcgat gatattggag agtttaactc
    21061 agaaaacttt atgaaaggtc tcttggaagg tttaatctaa tcagaagcaa aaatcctgca
    21121 aggcataaac ttgcaggaaa tttttttatt ctaagcgacc atcttgacga taggtgatat
    21181 ctggttgcca agtccatttg gcaccgaatt tttcaagtag gtcaaagctg gcttgaggtc
    21241 ccatgcttcc agctttatag tcatgaagtg gggcaccatt ttcagcccag agcttttcaa
    21301 tacggtcaat caacttccat gacgcacaaa cttcatccca gtggctaaag ttagttgagt
    21361 tgttatttag gacatcataa atcaattttt cgtatggttc tggagaagca ccagttgcag
    21421 tcgcatctgt acggtaatca agtgagttag gagccaagtt aaattcttct cctacttgct
    21481 tcccatttag gctaagagag aagccttctg ttggttgaat atagatggtc aaaatatttg
    21541 gagcaagtgg ttctccaaag atagaatcca tttgtttaaa gacgatgttg acatgagttc
    21601 ctttttcagt cagtcgttta cctgtacgga aaaagaaagg aacaccacgg aatcgatcgc
    21661 tgtctacaaa gaaggcacca gatgtaaagg tttcagttgt tgattctgga ttcacatttg
    21721 gctcgctacg ataagagatg tatttcatgc catcaatctt accagagcgg tattgcccac
    21781 ggataaagtg ttctttgagt tcttcatcag ttggatgata gaggttttta aagaccttaa
    21841 tcttttcagc acgaatctcg tcttttgtga agcttgctgg tttgtccatg gcgaggagcg
    21901 aaagaagttg tagagtgtgg ttttggacca tgtcacggag ggcaccggat tggtcatagt
    21961 agccaccacg ttcttctaca cccaagcgct ccgcaaaggt aatttgaaca ttgtcgataa
    22021 aatccttgtt ccaaacgttt tcaaaaatca agtttgcaaa gcgaactgca aagatgcttt
    22081 ggatcatttc cttaccaaga taatggtcga tacggaaaat ttgttcttcg tcaaatgttg
    22141 ctaggagttc gtcattcaac ttgtttgcag ttgcgtaatc tgtaccaaat ggtttttcaa
    22201 cgatcaagcg ctcaaaacct ttgccatcga caatgttttc agatttgagg tgtttggcaa
    22261 tggttccaaa gaactgaggt gccatagaca agaagaagag cttattgtgt tcagcttggt
    22321 atttttcatt aagctcagct tgtaattgac gcaaagcaat ataatgttcc gaatcattga
    22381 catcatggct ttgatagtag aagtggctag caaattcttg ggcttgctcg gtactatctg
    22441 ccaaatcaag gatggactcg acaactacag attcaaaata ttccttactc caaggtctac
    22501 gggcagttcc aataacggca aagtgcttgg aaagattgcc ggatttatat agtctaaaaa
    22561 gggaagggta gagcttgcgt ttagccaggt ctccactcgc accgaaaatt gtaacaataa
    22621 ccttagatga catctagcta cctaatttct atttttattc ctagtcttgc taggtatgag
    22681 gaaacctcat ttcataaact taattctaac ataattttcc gaaaaatcaa aaattccaat
    22741 acgaggtaga aaaactgcaa ggaaatcctt acagtttgag tttagacgtt taagacctta
    22801 tctaagaact ctttcagacg agggtgttgt gggttatcaa agatttggtc aggtgttccg
    22861 tcttcaagga actcgccatc tgcagtaaag ataacgcggt tggcaacctg acgagcaaat
    22921 cccatctcat gggttacgat aatcatggtc atgccttgct cagccaattc cttcataacg
    22981 ttaagtacgt ctccaaccat ctcagggtca agggcagaag ttggttcatc gaagagcatg
    23041 atgtctggat tcattgctag gccacgagct atggccacac gttgtttttg accacctgat
    23101 aggctatctg gattggcatt agctttatct gctagtccaa ccttttcaag caactccatt
    23161 cccaatttct cagcttcttc cttagtcatc aacttgtgct caataggagc aaaggtgatg
    23221 ttgtccaata cagacatatg agggaagagg ttgaagtgtt ggaataccat gccgatattt
    23281 tcacggacgt ggtcaacatt ggttgttttt tcagttaaat catagccgtt cacagtgatg
    23341 tgaccgctag tgacttcttc taaaagattg aggctacgga ggaaagttga cttaccagaa
    23401 cctgaaggac cgatgataca aacaacatct ccttcataga acttagtcgt aattcctttt
    23461 aggacttcat tttttccata gtgcttgtgt aaatcattta catcaatttt taattttgcc
    23521 attaacgaat cctcttttct aagcgtttcg ctagtctagt caaaagtgtg ataattacaa
    23581 gatagaagat agcaaggatt gcatacatct tgaaactttg gtagttacga gcaatgataa
    23641 tcttaccagt ttggaagagt tcaaccaaac cgatagcaga tacgatagtt gtatctttaa
    23701 gagcgataac gaattggttg acaaagtttg gcaacatcaa tttagttgct tgtggcaaga
    23761 taatcttacg catggttttt ccataagaga tacccaagct tcggctggct tccatttggc
    23821 caactggaac ggcctgaata ccaccacgaa cgatttcagc gatataagca gccgcattga
    23881 gtgagagggc aatggttcca gctacaaagt cgttaattgg gctttgttgg cctgtgatag
    23941 actcgatgaa gtttggaatt ccccagaaga tgaaggctgc aagaatcatc aatggaatac
    24001 cacgaataac gtcaacgaaa atctcagaga tgacgcgaag agatttgtat gggctaacgc
    24061 taaacatacc gaagataatt ccgatgacaa tggcaatagc aaatgagata agagctagag
    24121 caagagtgat accaagaccg ctaaggagtt gtttgtagtt gttttgaagc aagccccaga
    24181 gcgttgtttc gtcaacagta cttgttgaag cagttgaaga ttcgctagct aggtatttgt
    24241 caagaatctt ttggaattca ccgtttgctt taaggtttgc aagtccgttg ttgaacattt
    24301 caatcagttc tggatttgct ccttttttaa cggcaaaggc tgtttcaccg attggagttc
    24361 cagagattgg agttttcaat ttttgacctt ggctgataga atatttgaga acaggttcat
    24421 catccataac ggcatcaatg gcaccagtgt ttaaactgtc atacattgaa gaaccatcag
    24481 caaaggtttt gattttgtag ccgtatttgc tttgattttc tgttaggaag gtttgagaag
    24541 cagttccgtt tttaacaccg actgtctttc cttttagatc ttcataagaa gcaatattac
    24601 ttgattcttt gacaccaaga atggtattag cagtgtagta tgattctgag aagtcaaaag
    24661 ttgccttacg agcatctgtg acagacatac cagcgatgat accatcggct tgaccagctt
    24721 ggacagcact gatagcagca tcaaaaccag ggttggtgat ttcaatttca aaaccttggt
    24781 ctttagcgat tgccttaatc aattccatat caataccagt gtattgattg cttgaatttt
    24841 ggaaaacaaa aggtgcaaaa gaagaatcgc tggcaatgat atatttagcc ttaacaggaa
    24901 tagcttttaa tcctgcgaga gtagttgtag ttggcactgc tgaagatgat gaagcagtcc
    24961 atttcttgat aattttatca agactaccat cttttttcat ttcagccaag gcttggttaa
    25021 attcagtaac caggtgctcg tatttacttc cttttttcac accgaaagca aaacttccta
    25081 cagcttcacc atccatttca atatggaggt cttgaccttg gttaatggca tattcgataa
    25141 caggtttgtc atccatcatg gcatcgatgg caccagcact caagctgttg ttcattaaat
    25201 caccagtgtc aaatgtttta atagtaaagc cgtatttatc tttgattgtt tcaaggaaac
    25261 gttgagcggc agttccgttt ttaacaccaa cggttttgcc agttaattgg tcgtacttgc
    25321 taattttgtg tgactttgta gtagcaatga caacttttgt atcatagtaa gtatcagaca
    25381 tggtgaagac tttttcacgt tctttagtct ttgtcatccc tgccatgata gcgtcggctt
    25441 gcccagcttg aaccgcattg actgctgcgt caaatccagg ataggacatc tgaatgttcc
    25501 agcctttaat ctcagcgact ttgttaataa tgtcaacatc aattccttta taagtttgat
    25561 ctgaatcttt aaactcaaaa ggtgcatagg cggtatcaga aacaatctta atcgtttctg
    25621 ctttggcaat acctaatgag aaaattggga ataaaattag caaaaatgct agaaattttt
    25681 tcttcatttt tagtctcctt ttccgaatat tctctcatta tatcagaaaa agtaaaaaaa
    25741 ggcaaatttt tatatttttc gtgtcagaaa atataacatt gattttttcc ttttcttgaa
    25801 ttgctattaa aaag
//
LOCUS       DAVKJJ010000030        25427 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000030 DAVKJJ010000000
VERSION     DAVKJJ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25427
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.030"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(<1..152)
                     /locus_tag="VW144_001261"
     CDS             complement(<1..152)
                     /locus_tag="VW144_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU9600712.1"
                     /translation="MKKTIYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LQDSPGT"
     gene            complement(302..568)
                     /locus_tag="VW144_001262"
                     /pseudo
     CDS             complement(302..568)
                     /locus_tag="VW144_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(593..847)
                     /locus_tag="VW144_001263"
     CDS             complement(593..847)
                     /locus_tag="VW144_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU9600713.1"
                     /translation="MAANYLKAMQSYLKETDLPKLEQLVVTKSLWDTVDILDRVVGSL
                     VYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLAS"
     gene            complement(829..1070)
                     /locus_tag="VW144_001264"
                     /pseudo
     CDS             complement(829..1070)
                     /locus_tag="VW144_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(1071..1703)
                     /locus_tag="VW144_001265"
     CDS             complement(1071..1703)
                     /locus_tag="VW144_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9600714.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(1717..2712)
                     /locus_tag="VW144_001266"
     CDS             complement(1717..2712)
                     /locus_tag="VW144_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9600715.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENQSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(2709..3407)
                     /locus_tag="VW144_001267"
     CDS             complement(2709..3407)
                     /locus_tag="VW144_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEU9600716.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLIFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(3484..4494)
                     /locus_tag="VW144_001268"
     CDS             complement(3484..4494)
                     /locus_tag="VW144_001268"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEU9600717.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(4478..5485)
                     /locus_tag="VW144_001269"
     CDS             complement(4478..5485)
                     /locus_tag="VW144_001269"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEU9600718.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLTSSKETVTSLVEALEQGKSEKIIDQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQTVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(5472..6425)
                     /gene="mvaD"
                     /locus_tag="VW144_001270"
     CDS             complement(5472..6425)
                     /gene="mvaD"
                     /locus_tag="VW144_001270"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEU9600719.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(6407..7285)
                     /gene="mvk"
                     /locus_tag="VW144_001271"
     CDS             complement(6407..7285)
                     /gene="mvk"
                     /locus_tag="VW144_001271"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEU9600720.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(7407..8426)
                     /gene="cbpC"
                     /locus_tag="VW144_001272"
     CDS             complement(7407..8426)
                     /gene="cbpC"
                     /locus_tag="VW144_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEU9600721.1"
                     /translation="MKILKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDGGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDPTTGIM
                     QTGWQYLGNKWYYLRSSGAMATGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSS
                     GARVTGWVKDDSTWYYLNAGNGDMKTGWFQVNGRWYYAYSSGALAVNTTVDGYSVNYN
                     GEWVQ"
     gene            complement(8524..9213)
                     /locus_tag="VW144_001273"
     CDS             complement(8524..9213)
                     /locus_tag="VW144_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9600722.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            complement(9225..10649)
                     /gene="gndA"
                     /locus_tag="VW144_001274"
     CDS             complement(9225..10649)
                     /gene="gndA"
                     /locus_tag="VW144_001274"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEU9600723.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVSDVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            complement(10725..12119)
                     /gene="mapZ"
                     /locus_tag="VW144_001275"
     CDS             complement(10725..12119)
                     /gene="mapZ"
                     /locus_tag="VW144_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEU9600724.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLNQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            complement(12132..13289)
                     /locus_tag="VW144_001276"
     CDS             complement(12132..13289)
                     /locus_tag="VW144_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEU9600725.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            complement(13374..13754)
                     /gene="rnpB"
                     /locus_tag="VW144_001277"
     ncRNA           complement(13374..13754)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VW144_001277"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(13775..14104)
                     /gene="gpsB"
                     /locus_tag="VW144_001278"
     CDS             complement(13775..14104)
                     /gene="gpsB"
                     /locus_tag="VW144_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEU9600726.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            complement(14174..14701)
                     /locus_tag="VW144_001279"
     CDS             complement(14174..14701)
                     /locus_tag="VW144_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEU9600727.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            14767..15363
                     /gene="recU"
                     /locus_tag="VW144_001280"
     CDS             14767..15363
                     /gene="recU"
                     /locus_tag="VW144_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEU9600728.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            15360..17519
                     /locus_tag="VW144_001281"
     CDS             15360..17519
                     /locus_tag="VW144_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU9600729.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(17686..22989)
                     /locus_tag="VW144_001282"
     CDS             complement(17686..22989)
                     /locus_tag="VW144_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9600730.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQKGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYGNDRTVVSVKTDNQEGVKADDTPAQKETGSVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYTLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKIRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPRAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDTDNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQKLTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKRTALVADD
                     FESLDAPAQPGEGLENAFDGNVSSLWHTSWNGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKVQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPIVEKPEFKLNSLASEQGKTPDYKQEIA
                     RPETPAQILPATGESQSDTALFLAGVSLALSALFVVKTKKD"
     gene            complement(23290..25272)
                     /locus_tag="VW144_001283"
     CDS             complement(23290..25272)
                     /locus_tag="VW144_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9600731.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVN
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
BASE COUNT         7174 a         5884 c         4663 g         7706 t
ORIGIN      
        1 gttcctggac tatcttgtaa tgtctcactt acctcatatt cttcacctgt actagaaata
       61 acactcaaag cagatactgt cgataactgg ctagccaata aagtactcgc aataattgaa
      121 atacccaatt ttttataaat agttttcttc attattgtat cctcctaatg taattatagc
      181 gtactattct aaatttctta atctactata gaatcaagaa atctaccacc ttctttaaat
      241 accctccatt atcacataaa caggtaaact tttcaattaa tgactgcgct tttcaatcac
      301 gctagaggta cttgcttgct tctttgatac taagttcagc cattctttcc ttgtttttct
      361 caataaagca tgttacccaa gtgggattcg ttttggagta gtctcgcaga gtccagccaa
      421 tggctttatt gataaaaaat tctgtttggt tcaagttatg aaggagaatc ttttccatta
      481 attgagtatt ggtcttctct tttcttaaca actggtggtc aatagcgaca cgtctcagcc
      541 agatattatc tgataggctc cattttatac tcaatgaaaa tcaaagagca aactaggaag
      601 ctagccgcag gttgctcaaa acactgtttt gaggttgcag atagagctga cgtggtttga
      661 agagattttc gaagagtatt aagattattt cttctagttc agggtgttca tacaccaaac
      721 tccctactac tcgatctagg atatctaccg tgtcccacaa ggattttgtc acgactaact
      781 gctctagctt aggcaaatcg gtttccttta gataagactg cattgctttc aaatagttag
      841 cagccacata ttggtatttt ctaggatcct tttcccagca agtgtctgca aaatcccaat
      901 cgataatctt tgtttttttc gcttctggaa aatattttat agagtttatt tctttcaggc
      961 accgcaatac ctagaaaaga aaattgatgg cgcatatagg cttccatgga ccttgctttt
     1021 ttagagtctt ttgctgcttc tagctcctca agtaaatctg ctaaactcat ctaaaactcc
     1081 tcttgcccca ccaaatggtg ctgaaaggca tagacagccg cctgggtacg atcgctgact
     1141 tcaagtttgg caagaatatt ggacacgtgg gtcttgaccg tcttgagaga gataaagagg
     1201 tcatctgcga tgcgctgatt ttcgtagccc ttggcgatga gttggagaac atctcgctca
     1261 cgcgcagtca attcttcatg aagttccata tgattgcggt ggtattcaac cttcttgcta
     1321 acctcttgct caatggccag ctcgccagca gctaccttac tgacggcatg aagcaattca
     1381 tctgcactag aagtcttgag catatagcct ttggcaccag catctaagac tggcatgatt
     1441 ttttcattgt ccaaataaga ggtcacaatc aaaatcttgg cttcaggcca ttctttaagg
     1501 attgctaagg tcgcgtcaat cccattcatc tcaggcatga caatatccat gacaatgaca
     1561 tctggacgca gttccaaggc caagtcaatc ccttgagacc cgttggacgc ctcacccaca
     1621 acttctacat cgtcttggag gtcaaagtag cttttcaagc ccaatcggac catttcatgg
     1681 tcatctacta gtaaaatttt catctttact cctttatcat tccttatcta acaggggaat
     1741 acggatatca accgccagcc cttgcttggg agctgtcaag agttgaactg ttccagccat
     1801 atcttcaacc cgctccttga tatttcgcag tccataactc aagtcgtcta agctccctaa
     1861 ctggaaacca atcccattgt ccaccacctt cagttgcaat tcaacatctg tctgatagag
     1921 gtagacatct aggcaagatg cctgggcatg gcggagggta ttgctaatca actcttgcag
     1981 gatacggaag atatgctcct cgattttctt aggcaatttc gtcatattct gcttgagact
     2041 aaccctaaga tcactcttgt cctcaagctc ttttaaaaga atttgaatcc cttctatcaa
     2101 gctcttctgc tccagttcaa ctggtcgcaa atgcaagagc aaaacccgca aatccttctg
     2161 ggctgtttct aaaatagctg tgacactctg caactgggtc tgcatctttt ctctatccaa
     2221 tttcaaagcc tgctgactga tacccgataa aatcatgtgg gccgcaaaca actcctgact
     2281 gactgtatcg tgcaaatccc gagcaattcg cttccgttcc ttctcgatga tttcctcttc
     2341 ctgagcaagg ctctgatttt cagctttttg aagagcctct gtcaaaaggt taagtttacc
     2401 tgataaggac ttgaaactgg catccaaatc tggatctgca acctgaacca cttcttgccc
     2461 tgctaataaa cgcttgagat tagcctgcat ttttcttaga gaaagctctt cgatccctcg
     2521 ccaaaacagg gctaagagac aggtcatgga catgctgaaa accaacaata aaaagacaaa
     2581 tttttctgtt ttttcgacat cgtgcaaaaa gatagaccag tcaaaatcaa gtatttccag
     2641 caagctgtgg gagaaaaaaa agacaaatag gaaggaggtg agagcaataa tgacataggc
     2701 ttgttttttc atcctctaac cacctccaca tcaccaatca tagtggtcaa gaaaatcttg
     2761 acactcttgt tactcttgag atagtctttt gtttcttgat gatagtgttc attgcggagg
     2821 gctcgcttgg gctggttgaa aaaaatcaaa tccccataga gacagttaac gctgagactg
     2881 acttccacat ctacaggtac gatgattttg gtcgttccta ccatctttct gaggataatg
     2941 acattgtcat gattggttaa gatgaccctc tccagatgaa tagtgtcctt gcccatgaag
     3001 cgaaagagat tgatatcatc gaattggcaa gtctggtagc ttgaaaaatg atgaagattt
     3061 ccaaaccaac gatttttctc cttcttaacc gtcacgacct cttcaaaaac caaattggtc
     3121 tgctcttttt cctggttcat catcgggtaa agaagaaaga ggctatagat aaccgcaaca
     3181 aaaatagcta gaatcacaaa aggattgagc ataacgatga aaaagaagag aatggttgcc
     3241 gctactaaaa gaagattatt tccctcttta ccagtgtagt agcgaatcaa aagcaaaaag
     3301 aggaatagta tcagcagaaa acgcgaaaaa tgctctgata ccatcaaaat cagagctcct
     3361 gtcagaagac aggcttcgat aaataaaaag attttaaatt ttctcatagg ttcatcctct
     3421 cccttctatt ttatcacaat tcaaaaaagt cacctcagtc tgaggatgga aaaaaggcgc
     3481 tggttacgcc tttttcatct gatcctttgc ttcttttaat tttccataaa gaagatagtc
     3541 tactttttgt agatctgcta tggtggcaca gttaagggaa cacataatca agcgtagatc
     3601 tgctttccag ccttggacaa tgccaatcac ttcttcaact gtgtaggttt caaccaattc
     3661 cagaacggtt cgtgacaatc ccacagcctt agcaccaaaa accaagcact taatcatatc
     3721 cagcggattc cgaacccctc cactaaccaa gagttcgacc ttatctttcc attcttgggc
     3781 attgagaagg gcctgcatgg tagactgacc ccattgattg aggtaatcac gctggccact
     3841 acgacggttt tcgatatagg caaagctggt gccaccacga cccgataggt ccactgtacg
     3901 aacaccgaat tcataggctc tttcgattgt cttggcatcc attccaaagc ccacttcctt
     3961 gaggacaata ggaacgggaa tttgcttgct ataatctgct agatgcgatt gccagcttct
     4021 aaacttcctt tctccctcgg gcatgagtaa ttcctgcatg acattgacat gcacttgcaa
     4081 tagaacagga ttcatctctt ctacagtctg aagtcctaac tcgacaggct tgtccaatcc
     4141 aatattggtt ccaaggagga gattgggatg actagacttg acagaaaaag aatcatccgt
     4201 tggatttttg agggctgcgc tataagaacc cgttacaaat aaaataccac aggattccgc
     4261 cacctgagcc agcttttgat tgatttctct tcccttatta cttccaccag tcatggcatt
     4321 gatataaaaa ggaaagtccc actttcgacc agcaaactct gtcgaaagat cgatttcatc
     4381 cagattgtaa agaggcaagg aagaatgaat cagctccacc tcatcaaagc tattatagga
     4441 acttttctgc tcaagggcat agaggatatg ctcgtcctta cgatttgtcg tcatgtccta
     4501 tcctttcttg atataagagc tcaatcccca gatcggccca acgatttttt aaggttttgg
     4561 ttgattgcgc atcaaaactc agggcgatgc cacagtcacc accaccagca ccactactct
     4621 tggcaacggt ctgcaaatct tgactggctt ctttcaactg tctaagcaaa ggcgtgtaaa
     4681 tatctgtact caagccttct aaaagcttgc tggctacttc tacttgatcg ataatctttt
     4741 ctgatttccc ctgttccaag gcttctacca gagaagtcac cgtttctttt gaggaagtta
     4801 aaaaattttg attgatattt tgcttgattt gctggaccat gtgactcgat acagccactt
     4861 ccttggtcca tcccactaag aaatcacatt ctaaagttgg tttcacttgt gaaattgaaa
     4921 agccccaatc acgctccaga actgtcgcca agttttcttc ttctaaccaa gcagccacct
     4981 tctggcgatc aaatgactgg tagagaacca aatcctctgc cacaatacag gcaaggtccc
     5041 ccatggaacc attgtctcct cgcttgagca agacagcgct agtcagcttg aacaagagct
     5101 cctgatcaac agaaacatca tacagagcca gtaaagcctt gacaaccaag acaacgacgc
     5161 tgccactaga acctagacca aactttttcc cttctcgctc cattttgcca cagatttcta
     5221 gagaaaaagg tcttaaattc tgaccacgaa cagcgaggaa gtctcccatc aaagcaatcg
     5281 tttcttgaat caagctatag tcaggattgg gccttaagtc cactgcgaaa tcaaacatat
     5341 ctgaatagat acggtagctg tcagaaaaag caatctcagc cctcatatag atgggaatat
     5401 cctttatcaa agctaactgc cctggctcta aaatagcata ttcacctgcc caatagagtt
     5461 ttccgcaagt tttaacagca atcatcttga ctcaaatcct ttgtttttga cacaatcaag
     5521 cgataacgct gaccgaaaat ttctgataaa tgctccaagt ctttctcctg acagaagacc
     5581 ttaacattgg gaccagcatc catggtaaag tagcaggcct ctcctttctc acgaagctgg
     5641 cgaacaaagt ccatagcctc ataagaggca tccgtcagat aagaaaaggc tggactagca
     5701 gtctttgtcg tagcatgcat agccagggca tttttctccg ttaattctcc aatcttggca
     5761 aaatcatttt ccttgagata aatcagcata tcctgatagt ccttctcaga ctgacgaacc
     5821 cagtcgtcaa aagtcgtcga ggtttccaca caaagtttca tcccgtcacg gctagagatt
     5881 ggttttttct tgtcctctag caccaacata atcatagcta gtttcaagtc tgtctctaca
     5941 gggtaaattt ctccactatc cttatcccag gctcctagtg gtccataaaa actccgagaa
     6001 gaagaacctg aggcaaattt ggcttcctgt gccaactgac ttctatccaa tccaagcttg
     6061 aaataagcat tacaagcctt gaccagggcg gacaaaccac tagaacttga ggacagaccc
     6121 gctgccgtag gcatattgtt ttgagtatcg atacggacaa agccctcacc agctggacga
     6181 taacgatcaa taatcttact catcttggca tgctcgacct cattttgtag ctgaccattg
     6241 atgtaaaatt cgtcagctgt tacattggct ggtaaaggcg acaaggtcgt ctctgtatac
     6301 atattttcca aagttagaga aatactgcta gtagcaggca ccatctcttt ttcttttttc
     6361 tttccccaat atttgataat agcaatattt gcgtaggaac gtactgttac aggctctcta
     6421 tccatgtctg aacagctcct ttctcttcta atctttctgc tagttcttgt gcgtgtgtca
     6481 aattggttac caaggctatg atacaacctc ctagcccacc accgctcatc ttggcaccca
     6541 gagcaccatg gctaagagtc gtttcaacca aaaagtctgc ctcagggcta ctgactccaa
     6601 tttcttttaa atgtaaatgc gcttgactga ggatttgtcc cagtccttca gcatcttttt
     6661 gtgaaattgc aacttctgct tgctgggtta attctcccaa ggcatgcaaa aacggtaggg
     6721 catccttgcc cttattttga accacttgga tggcttcacg agtatgacca taaacacccg
     6781 tatcggcaat caccaaatag gcggataaat ccatctcaag ttctgtaaat cctacgttct
     6841 tgataaagcg aataggttgg tcactgagac aggtcttagc atccaaacca ctaggattca
     6901 tatgggcaat catttcagct cgattgacca agatttctaa tacatcatga ggcagatcag
     6961 cctgatagta gtcaaatacc gcacgaatgg ccgctatgct gatagccgct gacgaaccca
     7021 tcccccgttt ctcagggata gccgagtcaa tctcacaacg aatgcaggct tctgtgatat
     7081 ccaaatactc cagtgaggca taaaccgcca tggacaaggt atcctcctca taaaggcgcc
     7141 aaggactctc tgcaggaact accttacagg tcacctccac ctccaacaga ggcagggaaa
     7201 tggcaggata accgtaaacg accgcatgtt cccctattaa aattatctta ctatgtgcct
     7261 gaccgacacc aacttttttt gtcatttttt ccttttacta gacgaaaaaa cgtcttattt
     7321 ttcatacaag tattaattct ttcctatcta ttttattata ttttcacaaa aaaagcgatt
     7381 gtttccattc acaatcgctt ctttcattat tgaacccatt cgccattata gttgacagaa
     7441 tagccatcta cggtcgtatt cactgccaag gcacctgagc tataagcgta gtaccatctg
     7501 ccattgacct ggaaccaacc tgtcttcatg tctccattac ctgcatttag gtaataccaa
     7561 gttgagtcat ctttcaccca gccagtcacc ctggcgcctg acgaacggag atagtaccat
     7621 ttgttcccaa ggttttgcca tcctgttttc atatcgccat ttggctggtc taaataatac
     7681 caagtggtac cttcctgata ccagccagtg gccattgctc ctgaggaacg gaggtagtac
     7741 cacttattac ctagatattg ccaacctgtt tgcataatac cagttgttgg atctaggtag
     7801 taccatggag caggtttgct tctatcaatg tcaatagtaa catgaaaatc ttgagtccaa
     7861 cccttagcaa cttcaccaat tggtagtgga ttgtaagaat catcaccaaa atttcctagc
     7921 ttatttaaat aataccaatt gccgtcttca taaatccagc ctgtctttac agcataatta
     7981 ttatcaaaat aataagttct cttttcagca gatagaggat attgttcacc gtatactttt
     8041 cctgtatttt cagacgtttt tgcctctagt acttgtttat ctgtttgttc taacaaagca
     8101 ccatcttgtc caaaatagta ccacttctcc tgaaggccaa tttcattaac tggttggtta
     8161 tcgaataaat tatcacgata acctgttcca ggaatttcta agtattgcca accgacaacc
     8221 atctctcctc catcaccaaa atagtaggtt ttaccgtcta ttttatgcca atagattgct
     8281 ttatgatcat cttttacata atattttcta ttatccttat caacaaatcg gccacctgtg
     8341 gtattcgcaa ataccgtatt tgtagctagc aaaccaaaga aaaagactgc tagtagaact
     8401 tgcatcattt ttttcaaaat tttcatctat ataccctcct aaaacgattg tagcaaagac
     8461 ccgactgcat gtcaatatat aaaaactcct caaaaaagca gttacacaac tgcaactgct
     8521 tttctattct tgcatggtgt aaccaacacc acgcacggtt ttaatgtagc ttttttggcc
     8581 tttaacatca agcttgctcc gtagatagcg gatatagaca tctacgatat ttgtctcggt
     8641 cgcactttca tacttccaaa cactttccaa caattgctca cgagtcaata ctttcttgct
     8701 tcccataagt gtcgccaaaa ggtcatactc acggcgggtc agagcaatca tctcttcacc
     8761 acgataaacc gtgtgatgtt caacatctat cctaagattg cggtaggtcc ttggaacttt
     8821 catcagactg cagtgttgat caatgaagtc ccgacctcgg aagatcgccg aaatacgcgc
     8881 taccagattt tcgataagga ctggcttata gatgtatgaa actgcaaaac gctgaacaac
     8941 ttccagctct tcttgcaagt cttcccaatg atctaaaatc atgatgactg aggcaggttt
     9001 agttcgactc aatttttctg caaaatcctg agccatcata tctcccagat taacgttcaa
     9061 taaaatcaaa tcatagtctg tctgaagagc catggagagg gctttttgcc cctcctctac
     9121 cagatcaacc cgatactgct ctttctggag ttccaaactt aaaaaatgag ctagatttcg
     9181 ttctttctca agtaataaaa tccgtttccc catggctgac ctacttattt ttcgtcatac
     9241 caagagtagt ggaaggttcc ttctttgtct ttacgttggt aagtgtgagc accaaagtag
     9301 tcacgttgtg cttggatcaa gttagctgga aggtcagctg aacggtagct atcaaagtaa
     9361 gtaatagctg ctgagaaagt tggcactggc acaccagctt gaaccgcaag agctacgata
     9421 tcacgcactg cttgttgata cttagcagta acatccaaga agtattcatc caaaagaagg
     9481 ttggcaaggt ctgcatcacg gttgtaagca tctgtaatct tttgcaagaa acgagaacgg
     9541 atgatacagc catcacgcca gatagatgcg atatctgcaa atggcaagtt ccagttgttt
     9601 tctttagagg ctacacgcaa ttgagcaaat ccttgtgcat atgaaatgat ttttgagaag
     9661 taaagggctt gacggatttt ttcaatcaat tcagccttgt ctccttcaaa gttgaaggca
     9721 gctggttttg gaagcacctt gctagcatgt acacgttctt ccttgtaagt tgaaatgtag
     9781 cgtgcaaaca ctgactcagt aatcagtgac aatggtacac caaggtcaag agatgattgg
     9841 ctagtccatt taccagttcc cttgttacct gcagcatcaa ggatgtagtc tacgattggt
     9901 ccatcttggc cttcatcgtc tttacggctc aagatatcag ctgtgatttc gatcaaataa
     9961 ctgtctaatt cacccttgtt ccactcagta aagatttcag ccatatcctc tgcagaaagg
    10021 cctagcaagt gttgcatcaa gtcatagctt tctgcgatca attgcatatc accgtactca
    10081 ataccattgt gaaccatttt cacatagtga ccagctccat cagggccgat gtaagtcaca
    10141 catggtttgc catcttctgg tgctttagct gagatttctt caagaacatc cgaaaccaat
    10201 tcgtaggcct ctttttgtcc accaggcatg atagaaggac cttcaagggc acctttttca
    10261 ccaccagaaa ctccagtacc gataaagttg atacctgagt ttgccaattc ttcattacga
    10321 cggatggtat ctttgtagaa agtgtttcct ccgtcaatca agatatcacc cttgtcaagg
    10381 tgcggaagaa gggcttggat agtagcatct gtaccaggtc cagcttgaac catcagcatg
    10441 atacgacgag gtttttcgat tgagtttaca aaactttcaa cgtcatagct tggtacaaag
    10501 ttcttttcag gatggcaagc aatcacatct tccgtttttt ctttactacg gttgtagata
    10561 gcaactgtgt aaccacgaga ttcaatatta agggcaaggt tacgacccat tacggccata
    10621 cctacaacac caaagttagc ttttgtcatt tgacactcct cttgtttcat ttgttttatt
    10681 ttatcatttt tacttttgaa aagaaagaat tttgtaacga ctgcttagta gtccaagtca
    10741 tccgcatgac cagcgccatt tccgacaaaa tatcctgtct tacagttaag ggtaaagaga
    10801 taggttccat ctggcttata gaggttgtaa taaccattgc cgttaacgat attgacacgt
    10861 tcaaggatat attggtctcc agtgatatag ccacgtgaac gtgaagttgc tagaatttgt
    10921 tccaaaacac catccgcaaa atcccaggca gagttattac tatcatcaat agcagactga
    10981 ttataggcaa cacgactggc acttctttga acagcaaccc ctgcactcga gagacccata
    11041 ttgacttcac tgcgactact tctagtctca tttgaactac ttggttttgg ctcactagtc
    11101 gtatttgaac ttgcttgact tgaacttgag ctgctagttt gacttgaact tgagctagaa
    11161 gtacttgttt gctggctctt accaagactg atagccttat ctagcacttt atcaagctcc
    11221 gtatttccag ttttaatatc cgtaaattta gcatccgatt tggctttggc attggtatcc
    11281 aacacaccat ccacaatagc tggtttctca aattgagcat tgacatcttg aatagccttg
    11341 atttgcgttg ctagactatc atatttagat ttggcaagcg tatgttcacg actaccttcc
    11401 agcttatcaa gtaaagtctt gagttgattc agtttatcaa actggctatt tttcaaagcc
    11461 gttttattgc tatctgtgta aaaggcgtca taaagtgtat taaaatcatc cacatccgat
    11521 tgattggctg tagttgattg agaagtttca atttccttgg tcgaacgagc cacttgacgg
    11581 tagacataat aagcactgac acaaataatt actgatacaa gcgccaaaac ggtcaaaata
    11641 aagccttttt tacttttctt ttctgagtcc tcttgatcca gacgagaaag gttttgctcc
    11701 tcttccactt cttcagtcat ttctgttgga ttttcatcat ctagcaatag aggatccaag
    11761 ccttgctcct catcgtccag aggcaagggt agtaagatgt cttcagaaga tggaacttcc
    11821 tccgaagaag cttctgactt ctctacagcc tcacgcatct cttgaattaa atcatccaga
    11881 ctctgagttt caacgaactc ctcttttttg tattgacgag tcgcaaactt atccgcctca
    11941 atttcatctc tgtgttgctt aacatacttg tccaaaatgg aatcctcagg caagactcct
    12001 gcttccactt cttcattttt acgaatagct tgaccaactg ttagctcttt tgcttcatca
    12061 aaatcaaatt gcggttcttg accttctttt ttatgacgat ttcgtctttt tttactcatg
    12121 agtatccctt tctattttac ctcttgccgt ttgacacgct gaccaaaata ttgatataga
    12181 tccactttca aggttccgtt gtataactta cgcttcttat ctgcttggct accatacttg
    12241 ctttcaaaag cttcatcact agtcaggata aatttgctcc aagttttcag cggtgcaaat
    12301 acttgcccca tctcagcata gagcttggtc acccctgcat catctgacaa acgttcacca
    12361 taaggcggat tggaaatgat tactccattg attttatcgg aacgtaaatc ctgcacgcgc
    12421 atctgcttaa aagtaatgtc tcctgcaaca ccagctgcct gagcattggc cttagcaatt
    12481 tccaccatgc gagcatcaat atcacagccc atgatatcca gctcaagctc acggtctact
    12541 tttttagccg cttctgtgcg cacttcttga atcaagcgat cgctgatcca gttccattcc
    12601 tcaaatgcaa aagagcgacg aagacctggc gccatctttc tagcaatcat aactgcctca
    12661 atacagaaag tccccgaacc acaggtcgga tcaatcaaag gcttgtctgg ataccagtta
    12721 gaaagttgta aaatggctgc tgccatattt tccttgatag gagcgccacc tttttcggta
    12781 cgataaccac gtttaaagag gctagacccg gtcgtatcaa tcatgacagt tgccacatct
    12841 ttgagaatag agacctcaat cttaaactct gggccattct ccatcagagg aaccccttct
    12901 gggcgagcat agtgtttctg caatttcttg acaacagctt tcttagaaat agcctgaaca
    12961 ctgggctcat tgtgaagttt ggacttaaca catttagctt ttgaaatcgg gaaccgagct
    13021 ccaagtggta aataattttc ccaatctaaa gcgaaaactc cctgaaatag ctcttcaaaa
    13081 gtcttagctg ggaacgttcc tacgataatt ttgatacgat ctgctgcccg aagccaaagg
    13141 ttggtttcga taatagctct cacgtctcct tgaaaacgaa cacgtccatt ttcaacctga
    13201 caatcgtagc ccaactctcg cacttcacga ccaacgacag cctcaagccc tgctgccaca
    13261 gttgcaatta aattaaattc ttttttcatg ttacagtctt gcaagacctt tctatatgtc
    13321 cactttaaaa aatgaggttg agaaaatttc tcagccccaa cctatatgca attttctata
    13381 agccatgttt tgttccagaa gtgactagga ccacttcctt cgataatcat ctgtctacca
    13441 ctaacagctc tagctgatag cagtcagaat actacgttcg tttccgcgta ctccatgccc
    13501 cgaccaaaat ttgggttgct agcttgaggg gtttaccgcg ttccactctc tctgtttcca
    13561 gaaagactcc gtcactgtgg cactttcaag cctactctgg cctatccaag gacttagcca
    13621 tttcaactgc cgtaacgatt tctcgtccct aggcttatgg tttcgcctag cacaaacact
    13681 acaggcatca cagcctgtgc tagcatggac tttcctcatg agaagcaatt ctcctcacgc
    13741 gattatccaa aaattgcaca tctcaaataa ctacttaaaa atctgagtta tctaaaattt
    13801 gtttaccaaa aacttctttt tccaatctat tcaggcgttt caaaatatca aaattcgtca
    13861 tagaacttgt aattgccgct tcaaggggtt ctgcttgaac tggtgaaggt ttcggtttac
    13921 gagttaattc ttccttcaaa tccgcaattt cctgacgaag tgacttgacc aaggcagcat
    13981 aggtttcata gtccttgatg acatcgtcta aaaactcgtc aacttctact ttattatagc
    14041 cacggacttc acgtccaaac tcttgttcaa aaatatcttt cgctgaaaaa ataatacttg
    14101 ccatgtctct ctccattctc gcttgctagt attattatat aaaaaaaggc aaacaaaaat
    14161 caaggtcaaa gcttcaattt tcggaaaaat tttcagcaag ttcatttaat tgatcaaatg
    14221 ttaatctctt tataaaatag tcctcttgat ttttcatctt ttggtaaaaa taagctagtt
    14281 tcgtttcatt ttcttcatca taaaaaagat aggaactagt cgtgttttcc agcaaaaact
    14341 gctggtaatc tcttaactgc cccttgtgtt catagcgagg ataagcatat ttgacaaagt
    14401 ctacctgctt aaagcgactc agtttcatct gattgccttc attccaattt tccccatggg
    14461 tttcaaaagc aaaaatggtg gccaactgga aaccgtattc ggttttcatc tcctgagcaa
    14521 cctctaaaac ccaatattca aaccccaaac ttcctgtaaa cacaagccaa gacaccccat
    14581 ctgctgccat agcctctaaa tctttacgta tcgctttttt aatcaattta agacgaggat
    14641 ccttatcaga aaataaaccc aaatcaaaag cagaatagcc taaaaccaaa gctgtagcca
    14701 tttttaaccc tttctgtgca aattatggta taataaagtg atgttattgt accaataagg
    14761 agaattatgg tcaactatcc acataaagtt tcatcacaaa aaagacaaac atctctttct
    14821 caacccaaaa atttcgcaaa tcgaggaatg tcttttgaaa agatgatcaa tgctaccaac
    14881 gactactatt tgtctcaggg cttggctgtt atacataaga aaccaactcc tattcaaatc
    14941 gtacaagtgg actatccaca acgaagtcgt gccaagattg ttgaagccta ttttcgacaa
    15001 gcttcaacga cggactattc tggcgtttat aatggatatt acatcgactt tgaagtcaag
    15061 gaaacaaaac aaaaacgtgc gattccgatg aaaaattttc atccacatca gattcagcat
    15121 atggaacaag tccttgccca acaaggaatc tgctttgtcc ttcttcactt ttcttctcag
    15181 caagaaacct acttattgcc ggcattcgat ttgattcgct tctatcatca agataaggga
    15241 caaaaatcaa tgccacttga atatattcga gaatatggat atgaaatcaa ggctggtgcc
    15301 ttccctcaaa ttccttatct caatgttatc aaagaacatt tattaggtgg taaaacaaga
    15361 tgaacaaacc aacgattctg cgcctaatca agtatctgag cattagcttc ttaagcttgg
    15421 ttatcgcagc cattgtctta ggcggaggag tttttttcta ctacgttagc aaggctccta
    15481 gcctatccga gagtaaacta gttgcaacaa cttctagtaa aatctacgac aataaaaatc
    15541 aactcattgc tgacttgggt tctgaacgcc gcgtcaatgc ccaagctaat gatattccca
    15601 cagatttggt taaggcaatc gtttctatcg aagaccatcg cttcttcgac cacaggggga
    15661 ttgataccat ccgtatcctg ggagctttct tgcgcaatct gcaaagcaat tccctccaag
    15721 gtggatcaac tctcacccaa cagttgatta agttgactta cttttcaact tcgacttccg
    15781 accagactat ttctcgtaag gctcaggaag cttggttagc gattcagtta gaacaaaaag
    15841 caaccaagca agaaatcttg acctactata taaataaggt ctacatgtct aatggaaact
    15901 atggaatgca gacagcagct caaaactact atggtaaaga cctcaataat ttaagtttac
    15961 ctcagttagc cttgctggct ggaatgcctc aagcaccaaa ccaatatgac ccctattcac
    16021 atccagaagc agcccaagac cgccgaaact tggtcttatc tgaaatgaaa aatcaaggct
    16081 acatctctgc tgaacagtat gagaaagcag tcaatacacc aattactgat ggactacaaa
    16141 gtctcaaatc agcaagtaat taccctgctt acatggataa ttacctcaag gaagtcatca
    16201 atcaagttga agaagaaaca ggctataacc tgctcacaac tgggatggat gtctacacaa
    16261 atgtagacca agaagctcaa aaacatctgt gggatattta caatacagac gaatacgttg
    16321 cctatccaga cgatgaattg caagtcgctt ctaccattgt tgatgtttct aacggtaaag
    16381 tcattgccca gctaggagca cgccatcagt caagtaatgt ttccttcgga attaaccaag
    16441 cagtagaaac aaaccgcgac tggggatcaa ctatgaaacc gatcacagac tatgctcctg
    16501 ccttggagta cggtgtctac gattcaactg ctactatcgt tcacgatgag ccctataact
    16561 accctgggac aaatactcct gtttataact gggatagggg ctactttggc aacatcacct
    16621 tgcaatacgc cctgcaacaa tcgcgaaacg tcccagccgt ggaaactcta aacaaggtcg
    16681 gactcaaccg cgccaagact ttcctaaatg gtctaggaat cgactaccca agtattcact
    16741 actcaaatgc catttcaagt aacacaaccg aatcagacaa aaaatatgga gcaagtagtg
    16801 aaaagatggc tgctgcttac gctgcctttg caaatggtgg aacttactat aaaccaatgt
    16861 atatccataa agtcgtcttt agtgatggga gtgaaaaaga gttctctaat gtcggaactc
    16921 gtgccatgaa ggaaacgaca gcctatatga tgaccgacat gatgaaaaca gtcttgagtt
    16981 atggaactgg acgaaatgcc tatcttgctt ggctccctca ggctggtaaa acaggaacct
    17041 ctaactatac agacgaggaa attgaaaacc acatcaagac ctctcaattt gtagcacctg
    17101 acgaactatt tgctggctat acgcgtaaat attcaatggc tgtatggaca ggctattcta
    17161 accgtctgac accacttgta ggcaatggcc ttacggtcgc tgccaaagtt taccgctcta
    17221 tgatgaccta cctgtctgaa ggaagcaatc cagaggattg gaatatacca gaggggctct
    17281 acagaaatgg agaattcgta tttaaaaatg gtgctcgttc tacgtggaac tcacctgctc
    17341 cacaacaacc cccatcaact gaaagttcaa gctcatcatc agatagttca acttcacagt
    17401 ctagctcaac cactccaagc acaaataata gtacgactac caatcctaac aataatacgc
    17461 aacaatcaaa tacaacccct gatcaacaaa atcagaatcc tcaaccagca caaccataac
    17521 atttatcacc cagatttttc tgggtgattt tgtcatagca ctattgtatt tatgtgctta
    17581 acaaaagcct gagaagacat cttctcaggc tttttctgta ctataaacct cattggaaaa
    17641 gatagagact gcttccgtaa tcttgttaag aggttttact aaatactagt ctttcttcgt
    17701 ttttactaca aagagagcag atagggctag gctaacacct gctaggaaga gggctgtgtc
    17761 agattgactc tcacctgttg ctggcaagat ttgtgcaggt gtttctggtc tagctatttc
    17821 ttgcttataa tctggcgtct taccttgctc ggaagctaaa gagttaagtt taaactcagg
    17881 tttctctaca attggagcat ctggtttcgt agcagaatct tttaattggt tgagataatc
    17941 tgcaaagtat tccaccattc tttccgtcaa gagatggtta tccgtcgcat atttcatgct
    18001 tgcctgaacg ctagctactt cctcttgatt gtctttgtct gttaatttct gaaccttagc
    18061 caaagctgct tcatagcctg acaagtcaag aggtgtttct gctacctgtg gacgagtaaa
    18121 gataagttcc gctgcagatt ggtatttatc gccaccatct ccgtaagtct tagtacctgt
    18181 aaggacaatt ttcttagcct taattgtctt accaaaatca atgtcttttg gcttattgtt
    18241 atctggccaa tcagttgcag taaaggtatg ctccttgcca gactcatctg tcacaacaag
    18301 tttcacatca cgcaagttac catttgaacc tgatccacgt ggaacataac gaagtccagt
    18361 gatttcagtt ggttctttca agaccatggt tgcaggcttg cctacatctc caccattcca
    18421 agatgtatgc cataaactag acacattgcc atcaaaggca ttctcaagac cttcacctgg
    18481 ttgagctggc gcatccaaac tttcaaagtc atctgctacc aaagccgttc tcttctgaac
    18541 caaagcattc ttcaaggctt caatcttggc aatctctgca cgcgcttctt ccacactgat
    18601 atcatcatcg gcctgactga ggttaaagac cgcctctttc aaagcatcca tagactcttt
    18661 ggtgtagtta gtcatggcaa ccgttggcaa gtagttcttc agagcatttt ctgtcaacat
    18721 cttacctgtt agggtaattt cttcgatttg aagattatcc atcatgaagt cgttataacc
    18781 acggaagttg gcatttccac cagaatcacc acgagtatta cttgcatttc cagttgagta
    18841 gatacctacc caagtatctc ctgtttctgc acctgtcaca aggaaggttg ccttcttggc
    18901 tttcttagaa tctgtccaag tatttggcaa ttcatgcatt tccaagttgc ttgcttgagt
    18961 accacgacga cctgactgga attctccctt accgactaca aaagcatagg tattgtctga
    19021 acctgcttcg tattcaaagg ttacacggta ggtcttacct gcttcaaaac ggaagttttg
    19081 cggaatagtt tggtaaacca agttacgacg gctcactagt ccatttgtct tgagtgacca
    19141 atttccttcg ataacatcat cgactttctt accattccaa ccacgttgtg tatatggatc
    19201 gtgtttttca gacaagtgag tgcggttatc ttcaacacct tcaacaccac ccactacaaa
    19261 tgggaagata ccctgagcaa cattttcaaa gtcttgcttg aaggtgcctt tacctgtatc
    19321 atgcttgtct ccgtacatgc ttgaattgtt ttcaaaggta cgaatttcat caaagtaagt
    19381 tgcttgatca ccagcttcac gactcaatgt cagagtaaca tttgatacgt ccgatccagt
    19441 tgtaaagaag gcgtacatgt tttggaagta acttgtatcg tcaactgtag cattgttacg
    19501 acgtgtattg tgggcgtagg cttttacata gttgagggcg agagacttat tggtataagt
    19561 agtcacttct ttttcaccag tatttacagt gatacttgcc ttggcattac tacggttatc
    19621 gacaccgaca taaacggcat acttggtatt tggtttcaag ccagttaatt tctgagtgag
    19681 actaactttt tctttgtttc cttgaatacg aagcatatcg tttgcccctt gagacttgac
    19741 aatttctgcc ttagaagcat cgcctgaaat ggtccaatgt ttcaaggtac cgctattaaa
    19801 tccttggtca tagatgtgca tgccttcact ccatgacatt tcaggattag tttgcttcga
    19861 acgatagaga acgtatggtt gatttgctag aagatctagg gtaattttac catcttttac
    19921 agttagtttt tgctcttctg tcttaccttg gtcagttagc ttgtaaaggt aaaccttgct
    19981 ctttgcccaa tcgcttggaa gggtccaagt tgttgcaccg gcctgcgtat tgaagtagta
    20041 catcttttcc ttatcagtag aaagtttctt accatttgca tcccagttcc aaggagtcaa
    20101 gtaagctgaa ccatcttgga tgacacgtcc gttgagagtt actgtacgtt cgcgatattg
    20161 tgggctattg acatcatttg acttacgagt tacaactact ttattattgt cagtatctac
    20221 caattccact cgcatttctg gagtccattt ataggtgcta ccgttatcgg tcatagtcac
    20281 cggtgtacca ttttcccatt tacttacagt gaagtgttgg aagtacttag tcataacgtc
    20341 atgagcaaag aggttagtca cgtagccatt gtagtcactt cttccttgcc agccttcaaa
    20401 gtctttcatg ctgtagccac ctagcagtgg atagttggct gcaccaccat aacttctgta
    20461 gtccccaacc caagcatctt tttggtggtt acggataaag cgggtgatgg cactgttgat
    20521 acctttattg gtgtagccac cgtaggtcaa gtcagctgcc cagtgatgga aggtagagtc
    20581 gtactcacca ccatggcccc actcgatcgc aaagcgccag ccttgtttgt taatttcttt
    20641 agcaagaacg tgggtagccc aggcaccgtt atcacctgat tgaccattac cccaaacgtc
    20701 cacatagata aagtcgagac cgtcaccaag ttttttcttc aaatcttccc aacgtgccaa
    20761 acgaccatga gctaggtcat aggcagcatc aatgttgata ccttgatcta gccagttcca
    20821 accatagcta taacttccat ctggattctt acggagaatt ttttcattga agtatttaga
    20881 ctcaggataa gtttctgaag cgttaacgtg gatacctaga tgagctccat atttcttagc
    20941 cttctcaatt agggtcttga agtcttcgac accaccgata cgcttaccaa tatcagcata
    21001 gttcaagtga ccagagtcgt gaccttcact accatatcct ttaaggagaa caccttgccc
    21061 aagaccatct gtgtggagat tgattttctt gataccatcc aaggtcataa ggaatgggtt
    21121 ttgtgcttga gaaccaaagt tcatcgcgat acggtaagct gtgatatcct taactttttc
    21181 ccaaccttga gggttgttca taatgctacg ataagcaatg gcaccatcct gccaatcgac
    21241 tttcttgtct gcattggcat cttcagtgat aacaacctta gcccttggaa gttccttcgt
    21301 gtattctggg aaaacaatgc ccttataagc tttttcccat tgccattcag agctgtggat
    21361 tcctacatag ttggcattcc cgactgtttc tttataagct gtcaaacgag tccagtcatt
    21421 cgaaccacca ccatagctgt tttgagagtt actccaaaca ccagcagcaa gcttatctgt
    21481 agaaacaaat ccatacatgt aacccttagc caaatccttc attggattgg ttacatcgat
    21541 atgatcatct ccgctgacat gcgtattgtt tgacatggtt gccccatcaa acttagcacc
    21601 agtttgatca ctagaaacag agactaaagc attgccgagg aaactaatag aagaaagtag
    21661 ttttctttcg tcatcaatct tttgacctgg agtgacttga ttgtggttga caatcttggt
    21721 cacatcaaag tgcaattgat tgtccacaac ttgcaagcgt actgtcattt ccgcattgat
    21781 taagtgagca tcatcgcgaa tcttcatcaa gtactctgct gttgtctcat tgattttctt
    21841 ataagtgact tcaggggtga ttcggtggtt attgataaag acttggttga actgttgcac
    21901 ctgtcctggc aaagtatgtc cattcaaggt gtattccttg acacgaggga aggcttggtc
    21961 aattactgcc ttaagtacct tagactggat cgtatcataa gtcaccttgc tatcgtcaac
    22021 aaccgaacct gtttctttct gggcaggagt atcatccgct tttacgcctt cttgattgtc
    22081 tgttttaacg ctgacaaccg tacggtcatt gccataagag cccgccttga gaaggatctt
    22141 cttctcattt ttaagatggt cattgaccgc agctggtaga gtcactgtgt caaagagatt
    22201 gacatcgtta ttgctggcat ttagctgacc gtctgacttg agagtgatag agagacggtt
    22261 tgttgatcct gtttcaggag cagcaacacg actacctcta taccaagtgc tagttgttgg
    22321 agatttatac tcccagaacc agccatcctt gtcataaccg acaaaaacat tattattggt
    22381 atctttaaat ttcaggaaga caccaaagcg tgatttgccc ttttcagaat catctttgaa
    22441 ggttaaatca acagttgcat ttccattggc atcaacggtc aagcccttct tttcaaacag
    22501 ggctggttta cctgcgttat cattttgagc agttgaggat agttggttgt agcggacacc
    22561 tttttcttct cggatagtga ctgttccctt ttgttctttt ttctctaccg tttgccattc
    22621 aggggttacc gtcttaggtg tttcaggttt agcagctgca ggtttatcct cttttggttt
    22681 ttcttcagcc ggtttttctt tttcaagtgc taatagttct tcttctgtct taggtccatc
    22741 tgtaacttct ggagaacctt ggtcttctcc caccttaggc gcttcaaaat cacgtccatc
    22801 attctctttt gctgttgcaa gagcagtagc taaatcagct ggtaagttcg ccgtggaacc
    22861 agactgcacg ctatctgcaa gaaccggact tgtcccaaag aatgcagctc caatcataac
    22921 agaagcaaca cctaatgaaa atttccgaat actatattta caacgttttt caaataatcc
    22981 tttattcata aacttctaaa tcctttcatt ttttagcaag cgtttacatt tcctttttaa
    23041 aaaatactcc ttcctctgaa agtgcaatgc ttaaccctga taaataaaaa ataattatac
    23101 ttttaatata acaaataata gctgagaaag caataggaaa caagtaaaat taacaaaaat
    23161 tttctctatt ctgacaaaaa gaggctccaa cttttggggg tcagtacatt tagaatcttt
    23221 ctttacaaat gtaaaaaagg attcagcatt caagagaaat actaaatcct ttcttatatt
    23281 ttgcaacagt tatttcacat gttttgcgag atcttcttga gcctttttat tagactcttc
    23341 tttttctttc atccattttt cctgagcttt ttggtattca tctacagtga ctgccttgtc
    23401 ttgaagttcc aagtatttat acaagactgg ttcacttgtg cctttatttc ctgacaatgc
    23461 aaatggtatt gtaaatggta ccatcttaga caagattgga cgccctgtac gagatgtagt
    23521 tggaataatc aaagcactat ctgtcaacca agcttgggct gcagcgtatt tatcatagcg
    23581 tttagcaaca tctgtagcct catcaccagc ctcagtaacc aatttttcgt agtcatatag
    23641 acctactttt ttagcagcta cattatcttc ccctgagtca aaccctaaat atgttttagt
    23701 actttctcct acagatggtt tgatgatatc aaggtaggtt gatggatcgg caaagtctgg
    23761 accccaaccg acattatctg ataaatccca gtcttcgcca gcagcatttt cagcaaaata
    23821 tgtaatattg tttacttcgt ctttttgtag ttgttggata tcaataatga cattatcagc
    23881 tcctaaagtt gcttccaagg attgtttcat agattggacg cgctgaactt ttgtagttgc
    23941 tgtctggtca actggcatat ccaaatgaat tgggaaagtc acaccttctg cttgtaaggc
    24001 tgatttagct ttagcaaatt cagccttggc tttttctgga ttgtaaagac catcctgaga
    24061 atctgcaaga ttaacatcct tccattcatc cccataattg accaatttct ctttgaccat
    24121 atcgccaaag tttttaccat ctgcttgaac aaatgttggt ggaacaaaga tattacgtaa
    24181 gattttgctt gctccagttt gtccattcaa ctgagaggca taggctgtac gatcaaaacc
    24241 aaaggcaata gcctgacgga aatccttgtt taagagagcc tttttagtcg atgccttttg
    24301 ttcgtcgctg gtcttagatg tgtatttata ggactgacgg tcaatatttg taccaactaa
    24361 atacgtaata gagtcttgtt gagtatagac aatattgtcc ttcatactct tctcaagctc
    24421 tgcgaaactt gcacttgttg gatagagacg agctgctgta aggctaccat ctttaaagtt
    24481 ttctgcaggt ttgctggtat cttgaccatc ccagaatgac aatttaactt tgtcaatatg
    24541 cacattgtcc ttatcccagt agttcggatt tttcgcaaat tcaacagaag atttggtcac
    24601 aatggatttc aacaaataag gaccgttata caagagacta cttggatccg tagctttggc
    24661 aaaatcatcc ccttttgaat tcaaaaactc ttcattaact ggcgcaagca cacccatggt
    24721 tgtcttagaa ttccaaaagc tttctggttt gttcaaagtg tactgaactg tctgttcatc
    24781 cagagcctta attcctactt gtgagaaatc tttgatttcc ccttttacat aggcatccaa
    24841 ccctttgatt gattcttgaa caaggtaaag agcatctgat tttttatcag cagcatattt
    24901 tagtcctgtt acaaagtctt gagctttgac tgccgcgtat tcttcacctt cagaagtata
    24961 ccattttgca tccttacgga tagtataagt gtaagtcaat ccatccttgg atacagacca
    25021 atcctcagcc atagacggca caaagttccc gtagcgatca ttttctagca aaccatcaac
    25081 cacgttactg gtaatatttg ctgtcgcagc cttagcagtt gtcaaatagt tgaggttatc
    25141 agggtctgtc tcataaatgt atgagaatgt cttctcacct ttagtgcttg aacctgatcc
    25201 agagcatgca gctaaagtag tcgccgccaa taatgtcacg cccgcaaggg caaatagttt
    25261 tgaacttttc atcattttct cctttaaaac tttctctcca ttataaactc ttttcagaaa
    25321 gatgtcaaca gaattttcag aatttttgtg ttaatttggt atattctgaa aagacaaaaa
    25381 aagagaagac agtgattgtc ctctccagat gttagttgac aaagagc
//
LOCUS       DAVKJJ010000031        24624 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000031 DAVKJJ010000000
VERSION     DAVKJJ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24624
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.031"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(<1..201)
                     /locus_tag="VW144_001284"
     CDS             complement(<1..201)
                     /locus_tag="VW144_001284"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9600732.1"
                     /translation="MTLRTEDQVRDYAREVLGFNEVEENINQGTGQITTFNQLGFKGY
                     SDKPDGWYLPKNMNDVAIILETK"
     gene            complement(205..423)
                     /locus_tag="VW144_001285"
     CDS             complement(205..423)
                     /locus_tag="VW144_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9600733.1"
                     /translation="MVLNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
     gene            complement(669..947)
                     /locus_tag="VW144_001286"
     CDS             complement(669..947)
                     /locus_tag="VW144_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600734.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEMLELACWFRVIIVIEANSYV
                     NIRSSRLLPKFKTQKINSWCAAYSIHFNNGYCKQHTNEFKDSLKSLIL"
     gene            complement(1161..2792)
                     /gene="rlmD"
                     /locus_tag="VW144_001287"
     CDS             complement(1161..2792)
                     /gene="rlmD"
                     /locus_tag="VW144_001287"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9600735.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSLHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWCTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSQKNASLNKITNAHYVCDTAENAMKKWLKEGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2866..3840)
                     /locus_tag="VW144_001288"
     CDS             complement(2866..3840)
                     /locus_tag="VW144_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEU9600736.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(3914..4528)
                     /gene="pmp23"
                     /locus_tag="VW144_001289"
     CDS             complement(3914..4528)
                     /gene="pmp23"
                     /locus_tag="VW144_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEU9600737.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(4528..5502)
                     /locus_tag="VW144_001290"
     CDS             complement(4528..5502)
                     /locus_tag="VW144_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEU9600738.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQKENGTYSILIKNIEDIQSK"
     gene            complement(5510..6766)
                     /locus_tag="VW144_001291"
     CDS             complement(5510..6766)
                     /locus_tag="VW144_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU9600739.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            complement(6828..7259)
                     /locus_tag="VW144_001292"
     CDS             complement(6828..7259)
                     /locus_tag="VW144_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9600740.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(7261..7863)
                     /locus_tag="VW144_001293"
     CDS             complement(7261..7863)
                     /locus_tag="VW144_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEU9600741.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7847..8685)
                     /gene="prmC"
                     /locus_tag="VW144_001294"
                     /pseudo
     CDS             complement(7847..8685)
                     /gene="prmC"
                     /locus_tag="VW144_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
     gene            complement(8685..9764)
                     /gene="prfA"
                     /locus_tag="VW144_001295"
     CDS             complement(8685..9764)
                     /gene="prfA"
                     /locus_tag="VW144_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU9600742.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9774..10253)
                     /locus_tag="VW144_001296"
     CDS             complement(9774..10253)
                     /locus_tag="VW144_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9600743.1"
                     /translation="MTIELRDVTMENYFDVLNLDVKEYQKQFIATNAISLAEAYVYTK
                     NGDFVAPLAVYDNDVIIGFVMIAYDKKIVISSGNYLLFRFMIDKNFQNQGYFKPIMDK
                     VLDYVRTAPAGLSNKLWLSYEPENEQARFCYLSYGFKETGEISENEVVAIYDLTIEK"
     gene            complement(10243..10842)
                     /locus_tag="VW144_001297"
     CDS             complement(10243..10842)
                     /locus_tag="VW144_001297"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU9600744.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALTIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPMITSNKEQNYDN"
     gene            10965..11147
                     /locus_tag="VW144_001298"
     CDS             10965..11147
                     /locus_tag="VW144_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEU9600745.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            11284..12657
                     /gene="mnmE"
                     /locus_tag="VW144_001299"
     CDS             11284..12657
                     /gene="mnmE"
                     /locus_tag="VW144_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU9600746.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEEVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(12704..13639)
                     /gene="dapA"
                     /locus_tag="VW144_001300"
     CDS             complement(12704..13639)
                     /gene="dapA"
                     /locus_tag="VW144_001300"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU9600747.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     YPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAVLNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(13695..14771)
                     /locus_tag="VW144_001301"
     CDS             complement(13695..14771)
                     /locus_tag="VW144_001301"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU9600748.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPCDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(15357..15429)
                     /locus_tag="VW144_001302"
     tRNA            complement(15357..15429)
                     /locus_tag="VW144_001302"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15394..15396),aa:Thr,seq:ggt)
     gene            complement(15529..16038)
                     /locus_tag="VW144_001303"
     CDS             complement(15529..16038)
                     /locus_tag="VW144_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEU9600749.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(16036..16132)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            16161..16592
                     /locus_tag="VW144_001304"
     CDS             16161..16592
                     /locus_tag="VW144_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU9600750.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            16674..17051
                     /gene="mscL"
                     /locus_tag="VW144_001305"
     CDS             16674..17051
                     /gene="mscL"
                     /locus_tag="VW144_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU9600751.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(17093..18187)
                     /gene="hemH"
                     /locus_tag="VW144_001306"
     CDS             complement(17093..18187)
                     /gene="hemH"
                     /locus_tag="VW144_001306"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU9600752.1"
                     /translation="MKKAILMMTFGSPEEISFEGVAEFFTNIRRGVRPQDHEIQTLYD
                     NYIRIGGTPLQRITREEVNLVKERLGEEYGIYFANKFSRPFIPDVIKQMETDGVEECI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEVLLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            complement(18265..19097)
                     /gene="srtG2"
                     /locus_tag="VW144_001307"
                     /pseudo
     CDS             complement(18265..19097)
                     /gene="srtG2"
                     /locus_tag="VW144_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218039.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG2"
     gene            complement(19175..20173)
                     /gene="srtG1"
                     /locus_tag="VW144_001308"
     CDS             complement(19175..20173)
                     /gene="srtG1"
                     /locus_tag="VW144_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG1"
                     /protein_id="HEU9600753.1"
                     /translation="MKTKREKPKKSLSRRLVLAVDGVINHLLLIFAALIFLFGFYALW
                     DSNQVYSLASSSEYEAYRPVTTQQDELASFSGFSKLQELNPEVLGWINVYGTNIDYPL
                     VQAKDNEKYLNKDSKGEFAATGAIFLDARNNPKFEDFNTIIYGHHVENGVMFGDVAKF
                     ADQEFFDQHRYGSIYYNGVEKGLEIFEMLEVDAYDFNIYDPGIQGEDRQQAYLDHLLS
                     VAMHKRDISLSPSDRIILLSTCFLDVTNGRHIVVAKITDTVPKNTFHTKKSKPFPYSV
                     FDDSSLGRFLSSIPLWIWYLILFVLFLLLIFLLLVLYLILRRRRESKKNARSRPFY"
     gene            complement(20191..21387)
                     /gene="pitB"
                     /locus_tag="VW144_001309"
     CDS             complement(20191..21387)
                     /gene="pitB"
                     /locus_tag="VW144_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus major pilin PitB"
                     /protein_id="HEU9600754.1"
                     /translation="MKKTFFKKLFTASIAAITALSVFRGVPTFVDDNSAITKANGENN
                     AVVKINKTLNIAEGITTPTATFTFKFTEKTGQSSNGAPYQTGVAIPDRNVEYNKNDHP
                     TADKIQKATEDIFSGVAYGHAGEYVYDVAEAKTGWQAITKNGKTIDAMRYDKRTYEMH
                     VIVKNKVNGGVYISSVYFKENNKSNAPKVEPSEQGVYNLFDNTYTKDASKEPNPDDPS
                     QVDPNAKALTITKKVDGASGDKTRDFQFHIKIQLPSTNKTAETPVTNIIVKHGSKSEV
                     LAVVTPADTVEYNFTLKDGETFTVEQLPAGSKYTVTETGVAGYTDSSIYTTNGAEQTS
                     QGQKNVDFTLTDILIGEKKNDNKVTNKIDDVTPTGLLIDNLPFILMIGLGLAGFVVLS
                     KKRREA"
     gene            complement(21432..21983)
                     /gene="sipA"
                     /locus_tag="VW144_001310"
     CDS             complement(21432..21983)
                     /gene="sipA"
                     /locus_tag="VW144_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system signal peptidase SipA"
                     /protein_id="HEU9600755.1"
                     /translation="MLLKKKHKKPVTQVNRDKSPPSVWGDILYLVSKLLMVGFVLAIL
                     YFFVFGLLRYNDDGMKPALKDGDLVVYYRLDKRYSIGDLLVYSYKGKERVARVIATEG
                     STIDINENGLIINGSPQQEQDIYKETLLYKEGATFPMKVPAGQLFVLGDNRTTAVDSR
                     AFGTIPIQDTQGKVVTVIRRRGF"
     gene            complement(21983..23935)
                     /locus_tag="VW144_001311"
     CDS             complement(21983..23935)
                     /locus_tag="VW144_001311"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU9600756.1"
                     /translation="MDAGIGTGTNINAGLTEAQRLLQSARAGAKKVVILLSDGEANMY
                     YESNSGRTIYNYYSNPNVGRMIDTPYWFTSGLERGMLNISSLIAPKIDGFYSIKFRYI
                     GSNDSITSLKGYISGYNSGIPNEIFSANNENDLQQKFKEITDKILPLGVHHVTISDVL
                     SKYVQLLPGDASHLRVVKIKDGNEQELNDNQVTIETKKNEQGLVEVTAKFNPSYTLED
                     DAKYVLKFTVTSSQEAFDAIAGDKTLTSDDAEEADATKLYSNKGAKVAYSYGIGTSRT
                     KIKDYSEKPTFKPSDPLTVPVEIEWKGVDGKSNPSANRPPSVELNLNQKKDGSIKDSY
                     RKVTSPVQTNSFTENTSFAKVAKGYDYELKAPDAPGYTVEVQKTGTKEKPSFKVIYRQ
                     LPSLTVKKILEGEQSPNKSFTINVTFSDKDGKPINGKFGNTTVTNGKAQISLKNSQET
                     ALSYLPRDTHYKVEEVENSRTGYHVTYEKQEGTLSEDVQTIVTNHRLPTLSVTKKVTG
                     AFANLLQSFKITINVKDAQNKPLNGSYSAIVNNQKTTLQFTNGKATVDLKKDKTIKIL
                     DLPLNARYSIEEEASSSRGYQVSYDKKEGTLDANKSATVTNNKNSVPETGIDFLSSTL
                     VLGVVLPLGGIFFIILLGHLVVNRRK"
     gene            complement(24065..24568)
                     /locus_tag="VW144_001312"
     CDS             complement(24065..24568)
                     /locus_tag="VW144_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEU9600757.1"
                     /translation="MNVQYDFKKIQYFTSSLVIFLAILFLCAPINSLRADSITEPQTT
                     LHKTITPISGQKDQYELSLDITSKLGTETQSEPLDVVLVADLSGSMEERDVWSYSSRR
                     YISRIEALKHTLKGVNGRQGLIDTILSNSQNRLSIVGFAGKIDNQYNDRYYNEYYLSY
                     QYGTWPN"
BASE COUNT         7070 a         5173 c         4551 g         7830 t
ORIGIN      
        1 ttttgtttca aggattattg ctacatcatt catattttta ggtaaatacc aaccatctgg
       61 cttatctgaa tatcccttga agcctaattg attaaaagta gttatttgac cagtaccttg
      121 attgatgttt tcttcaactt cattaaagcc taatacttct cttgcataat ccctaacttg
      181 atcttctgtc ctcaaagtca ttttctattc ctcatcacta aaattatctt caaaactaat
      241 aatatcatta ggagtgcaag ataaagcctt acatattctt tctaaattct taaatgtaat
      301 tggtttatcc tttcccattt gtgccataac atttgttgta agtcctgcca tagctattac
      361 atctgttttc tttagtcctt tttttgctaa ctgtatccat aatggtttat agttaagtac
      421 cataatatcc ctcttttttt cttcattaaa tttatttaat tatagcataa atattgattt
      481 cattcaatct tacattgatt tagtgatttg ttttagagaa tttgttgtgt tattccgtcc
      541 ttattttgcc ataaattgtt ttaagtggga cctcttatcc ttaccatttt tacctcctaa
      601 ttttgagtat aaaaaagacg atagagtttt taatttctat cgtctgagat ttacatatta
      661 aattttcatc acaaaataag actctttaaa gaatctttaa attcattggt atgctgctta
      721 caatatccat tattaaaatg gatactataa gcagcacacc aactatttat tttttgagtt
      781 ttaaactttg gtagaagtct tgaactcctt atatttacat aactatttgc ctctataact
      841 attatgacac gaaaccaaca agcaagttca agcatctcca tatctttatt caaatgagga
      901 ctaagatatt cacaaacttt atcataaacc tctttatctt ttttcataaa aggggcatac
      961 aacttagcaa atttatccta aaattttttg ttgcctttat caatcatttc aacgctcctt
     1021 taaatataaa aataatccta tcaatcttta tattgagcct attttctaat tgataccttg
     1081 caagaaagga ataatagctg ttcctatacc aagtaggaat gtaataaatg cagaaagtca
     1141 tatactgtca ttcttttcta ttaaatcatt ttgaagtgtc tcaaagcatc catgatggct
     1201 tcttcttttt caggtgtaca ctgtggaata atttgtttat cctttttaga cttgttgtaa
     1261 tgttctcgta attctattcc acatttcttt tttatctgtg caatatataa tgtcgaaact
     1321 tttaattcaa atttattcca aacatattct ttgatttgag catatgttgc tttgctctcc
     1381 gcacttgtca aatccatctc atccagctca atttcaacac ttatgtgctt atcgacatcg
     1441 agtttggaca aaagtgctac cgtctcgacg tgatgcgttt gaggaaatag atccaccggc
     1501 tggactttct tcaattcata tcccaattct tggtagagtt tgatatcacg cgccatggtt
     1561 gcgacattgc aggagatata ggcgatgcga tcggctcctg tttgggcgct tgctttgata
     1621 aagctttctg tcaagccctt gcgtggagga tcaaccaaga taacggttgg ttgaatacct
     1681 tccttgagcc atttcttcat ggcattttca gccgtgtcac agacatagtg ggcattagta
     1741 atcttgttca aagaagcatt cttctggcta ttctcaaccg cttctggaat cagttcaaca
     1801 ccgtagactt ctttgacatg cttggcgact gataaaccaa tggttccaat accagaataa
     1861 gcatcaataa ccacgtcatc tttttttaac tctgcaaagt caatggctgt ttgatagagt
     1921 ttctccgcca tttcagtatt gacttggtaa aaggctgggc cagcgatttg gaagtcattt
     1981 cccaacatct ggtccgtaat atagtcttga ccataaagag tgcaccactc cttaccaaaa
     2041 atcgcattgg tattctggtc gttgatattt tgcatgacag acacaatctc tgggaactgc
     2101 ttgataactt gttcaatcaa ttggtcaaca cgaaaaactt ttggacgagt tgtcaccaaa
     2161 acgaccatga tttgtcctga atagtgacca cgacgcacca caagattccg aatcaatcca
     2221 gactgttcct tttcgtcata aggttttaaa tcaaaacgac ggagcaggtc tcgaagagct
     2281 actacgactt ggtcaatgac aggatcctgg ataaagaaat cttcaagggg catgaggttg
     2341 tgcgaattct tacggaaaaa tcctgtttcc aagacaccat tcactcgacg aacgggcacc
     2401 tgcgccttat tgcgatactt gactggatgt tccataccaa gcgtttcagc aacttctaca
     2461 tctgcaattc cagcaatctt gtagagactg tccttgactt gcttggtttt aaacttgagc
     2521 tgttctggat aagaaaggtg tcctaaatcc gcgattcctg aacgcaggta agccaaatct
     2581 agatcttgat tacggtgtag tgactggaca aggtattttt caacttttcc aaagccaatc
     2641 tttttattga ccttgaggac acgcatgaga attttttcac tcggtaaagc attctctaca
     2701 aaaaagacca aaccatctac cttggcaact cctgcccctt catgggtcaa atcaacaatt
     2761 tcaacttcta caatatcatt tttctttaac attcttttct ctttctattg gccgacaaaa
     2821 aagacggcaa cgttacgatt accatccatt ataccatgaa atttcttaag aaccaaaact
     2881 cttgaagaag ttgacaaggg cttgccagag ggagcttaag aagttaccac cgtcctctag
     2941 cgccttatcc gcatcaaagt taaggttgat atttttaaaa ctgtcgccag cttgtgatac
     3001 gatgctttgt ttaaggtcat ttagggtttt agtgaaatct gcattgctga ggatatcact
     3061 ctttgagaga ttcaaggcaa aattgatgat gatattgatc tggtttcctg ttatgacctg
     3121 atcaagtttg taatttttta aggtatcttc aacaatcttg cggacatctt cttctgtcag
     3181 atttcccttg ctttctttag ctttggcgag tcctgacttg atatcagcta gggcaacgtt
     3241 taatttatta gcatcatagc ctgatttgtc cttgttttca gcattgatat ctgacaaagc
     3301 ctttagctct tcttgagcca aatctttatt ggcttgcggc accttggctc cattagcttc
     3361 tagcgaatag tagatcccag ccaaagcact ctcacctgta actggaatag gggctgctac
     3421 agtgattttg gcatgttcca tacccagcgt tactgctgcg tttcggtaca tatcctgagt
     3481 caccttagtg atattttctg gtgtttcaat cttgacctca agtggcgatt tgtcacctag
     3541 cttttgaatc ttggctgatg aatacaactg taagctagag tcattggcca cattcatgat
     3601 tttagaataa acatcaggtg tcatggtctt gagttctttg gtatctgttg aggcattgta
     3661 gcccagtttt ttaagagttt gatttttttg gtcttcagat agggaggaac ctaggacata
     3721 ttcaggttgg acataggttt catcgataac tttttgaaca tctgttgctg catggacgct
     3781 attcatagct gttactgccc acaagaccgc agcactggtc agaaagagtt tctttctcat
     3841 agggaatttc ctcctttact tttctagagt aatatatcta tcttaaagaa aacttataac
     3901 aaaaacacct ggtctagcca gatgttgaaa agagagtgaa acatttgatg atgtaaaggt
     3961 taagtcgtac ctgtctagaa taataatagt ttcctccatt tacatagagt tcagcaccgt
     4021 gaaaaatgga aatggggtga atataactat aagtctttcc agtcgtatta ccaagcaagg
     4081 gggcaacagt ctcacgagag tactgtttgg ctagagccaa ggtattttcc ttgccatttt
     4141 gggcgataaa atcgatatag gcaggtccaa aattataggc ttgaacagct gtccagatat
     4201 ctaccccctt cttctgcgcc agatagagat tgcctgtcag agtttgaatg ccttgccgaa
     4261 tgctagaggc attatcattg atggtgttgg ttgaaccact tgcagactca ctagactgca
     4321 taacatcgcc ttcttttcct tttgtttcag tataaatcat agcaagcaca agctcttcgt
     4381 ttgctggggt gtctttttca ctcaagattt ctcgcaccat gggttgatag gtcatgactt
     4441 gtttgacatc ttgatgaacg cggtaagctt tatagccagc aaaaaggaag actgctagta
     4501 caagcactct tcgaattcgt ttaaacatta tttactttgg atatcctcga tatttttgat
     4561 taagatagag taggttccat tttccttttg gataaactca acagactcgg cgtcttgata
     4621 gacgttattg ggaacgatga gctcaattcc atttgataag gagagttttt ggttttcaaa
     4681 tttctttaat tggcgactgg catcaatttc atcaaattga acaggttctg gtacggcttc
     4741 tttgacttgg tcaataaagc tcaaacgagc cgtcagattg ttgtcaaaaa ggtcattagc
     4801 caatttctca ggtgacaatt cattgctttc ttctaggttg ttgaaaatcg ctgatttgac
     4861 cttggattga aattgaaaat catctgtgtt aaaagattca gcaattctct gggctgtttt
     4921 ttccagttcc ttgatagatt ttttaggaga aatcttagga gcgacagcaa gaagattatc
     4981 tgaaaaatag ttcaaaaaag tcccgttgta cttgattcgt ttttcaatca ggtgatactt
     5041 gcgactctga agattgacca ccaaggcctc gtcagcaccc gttccaaatc caggcaggtt
     5101 attctgagtc agcttgattg gattatcaac ttctcctccg aggtgggtca aggtctcccg
     5161 cagggcaatt cgcaagaaag cgaaatgttc tacaccttct ttagaaaatt gaacaaaaat
     5221 caagtcattg gtcttgagat tttcagaaat gctaaactcc tctttccaga gattagccag
     5281 cgttactgat gtctccaaca aatcgtctgt aatatgattg aagaagggat tttcttcttc
     5341 gaaaatccca gtcttggctt catctgaata cacatgttca atttttttac gtaggtattc
     5401 ttcgattttt ggagtaatat tgagaaactt atctgctaag aacagctcgg tatcatccgg
     5461 actgaactgg tgaataatgg ctttcttaat ataaatgtcc ataaaagttt tattcctcgt
     5521 ataatgggaa ggcatctgtc aattctttga ctgcacttct cacttcttct aatacagcct
     5581 cattttctga attcttaagg gttttaatga tgagttcagc cactttgcga ctttcttctt
     5641 caccaaatcc acgtgcagta atagctgctg ctccgatacg aatcccactt gtcttgaatg
     5701 gtgacaaggt ttcgtaaggg attgaatttt tatttagggt aatattgact tcatccagca
     5761 agttttgagc aacttttccg ttttctacaa ccttagtcac atcaacaagg aagagatggt
     5821 tttcagttcc acctgaaata atacggaaat cagggtcttg caagaagaca tctgccatag
     5881 ccttgctgtt cttaattaca ttggcagcat attccttgaa ggctggatcc aaaacttctt
     5941 tgaaggaaac tgccttagcc gccacaacat gctctaaagg accgccctga atacctggga
     6001 aaatagctga attgattttt ttagcaagtt cttcgtcatt ggtcaaaatc aaaccaccac
     6061 gaggtccacg aagggttttg tgggtcgttg ttgtagtgat atgagcgtat ggcactgggc
     6121 ttgggtgaag accagctgca accaagccag cgatgtgggc catatcaacc ataagcttcg
     6181 caccgacagc atctgcgatt tcacggaatt ttgaaaagtc aataatttga gaataggctg
     6241 aagcaccagc tacaatcagt tttggtttta cttcttgggc ttgtttcaag atagcatcaa
     6301 agtctaagag ttccgtttca ggatcaacac tataagaaac aaagttgtag gtttgaccag
     6361 agaagctaac aggagcccca tgagtcaaat gaccacctga tgccaaatcc attcccataa
     6421 ccgtatcacc tggctcaatc aaggacatgt aagccgcaca gttggcctga cttcctgagt
     6481 gaggttggac attggcaaat ttagcaccga aaatttcttt tgcgcgttca atagcaagag
     6541 tctctacaac gtctactaca tcagttccac cataataacg gcgtcctggg taaccctcgg
     6601 catatttatt tgtcaagata gacccttgag ctgccataac agccttggaa actacgtttt
     6661 ccgaagcaat taactcgata ttattttgtt ggcgttcttc ttctttggca atagcattcc
     6721 agagatcagc atcatatgct ttaaaatcat ctttgtcaaa aatcataggt cttctccttt
     6781 attgtgtgac tagtccatta gtttgatttt acaataagaa aatcaaacta aaagatgcga
     6841 ataaaccgtt tctgcatttt atcacaagta tagccaactt tttcataaaa tgcatgagca
     6901 cccagacgat gattggcaga atttaagcgg ataaacccat aaccacatct tttggcttct
     6961 tgttccaacc cttgtagtaa acttttaccg ataccttgac cttgcgcttg aggtgaaact
     7021 gctaaagcta agatattaaa tcctgctttg gaatagagtg attcgtaaac ttcagcgtgg
     7081 acatatccaa gtaagacatg attagctgca tcctcatagc caagtaggaa atgatgggaa
     7141 tcctgagaca gtctagctag ttggctagcc gtttcctctg gactaaaagt ataacccaaa
     7201 gcctcttggt tgatgtcaca tatcgctttc acatctgttt cttgcaaatc tcttagcatt
     7261 tcaagcctcc tcaaaagaaa tctctggcaa ccgagcaaga atatcttctc gtttaattgc
     7321 gccttggcgt aagattttca ccttgtctcc agacaaatcc acaatagttg aatcctgtcc
     7381 agttagaaaa gcatcgtctt ccagacccag aacctcttgg tcaaaatcct tcagaatttg
     7441 ttcaaaggtt acaccacttg cctgacctga gatattggca gacggcccaa tcaagggacc
     7501 tgtctctcga attaaatcca gtgtgatagg gtgactgggc atccgaaatc caatagttgc
     7561 aaggtcagaa tttacccaat agggaactcg gtcattggct tcgagaataa tggtcaaggg
     7621 acctggcaaa aaggtctcta caagtttttg tagataagtt ggctgattct tagaaaagtg
     7681 caagatatcc tcgaaagagg cgatattgag attgagtgcc ttatctctag gacgacgttt
     7741 gagttggtaa acatggtcaa ctgctttttc atctaaggcc ttggcaaaaa gaccataaac
     7801 agtctctgta ggtagaacga cagctccacc attttccaac tcttgtctaa tcctgtccat
     7861 catcaaccac aaccatccta ttttgaccaa attggtcttt gagtgttcga actttttttc
     7921 aggaagatgt tttctaaaaa gttcgggaac actttgacct tgcttgtatc caatttcaag
     7981 gtaaatctta ccaccatcct tgagatagtc ttttgcatct tccgcaattc tacagtaaat
     8041 agctaggcca tcctcgtctg caaagagagc tagatgaggc tccgaatgca aaacattcaa
     8101 gcctacctct gactcatctt cacgagagat atagggtgga ttggatacaa ttatatcata
     8161 tttttcagaa atttctgcaa aacaatcaga ttttttaaaa aatatattaa gattttgatc
     8221 gctagcattc tctgatgcaa gctctaaggc ctcttgagaa acatctgctg ctgtcactga
     8281 ccaatctggt ctattttttg ctagagcgag agcaatagct ccactacctg ttccaatatc
     8341 cagaactgag agattcgtct caagattttc agttagaata agctccacta actcctctgt
     8401 ttctggacga ggaatcaaaa cccgttcatc cacctttaaa tgcattccat aaaaatctgc
     8461 ctgtccaatg atgtactgag ctggcttgtg agctgctagt tgctggtaaa tatcttctac
     8521 aaattgtttt tcttcctctg ttgtcacctc ctgctggagg gcaaaaataa agtctgtaaa
     8581 agatagattt ttcagactac gatagacaaa agagaggctt tccgcttcct ctccttgtct
     8641 tatcaactct tcttcaaaat ttgaaaataa ttgagctaat ttcattattt gtttaattct
     8701 tctagttttt gtgtttggtc ataaagcacc aaggcatcca caacttcgtc caatttacca
     8761 gacaaaatcg tatctagttt ttggagggtc aagccgatac ggtggtctgt gacacggttt
     8821 tgagggaagt tataagttcg gatccgttct gaacggtcac cagtaccgat tgtcgactta
     8881 cgctcagcgt cttgttcatc ctgagcaatc tgagcaaagt ggtcagcgac gcgtgcacgg
     8941 ataatcttca tagccttctc acggttcttc tgctgggtac gttcttcctg catctcaacc
     9001 ttgatattgg ttggcaagtg aacgatacga acggcagtcg caaccttatt gacgttctgt
     9061 ccaccagcac ctgatgcgtg gtaaatatca acacgaagat cttttggatc gatatcgtat
     9121 tctacttctt cgacttcagg catgacaaga actgtggcag ttgaggtatg aacacgacct
     9181 tggctttctg tcacggggac acgctgcaca cggtgggcac ctgattcgta cttaagctta
     9241 gagtatacag actgacctga aaccatagca accacttctt taaacccacc gacaccattc
     9301 atagaggctt ccatgacttc aaagcgccag ccttgggctt ccgcatactt ttggtacata
     9361 gttagcaaat ctccagcgaa aagtgccgct tcgtctccac cagctgctcc acggatttca
     9421 aggatgatat tcttgtcatc gtttggatcc tttggaagga gcaaaatttt cagtttttct
     9481 tcatattctt ctttttcagc cttggcatct ttgagttctt gcttggccat ttcttccaag
     9541 tccgcatctc cgcctgattc cttaatcatc tcttcggcat cgacgatatt ttgaaggact
     9601 tgtttatact cacggtaggc tattacggta tcacgattgg aagcttcttc ttttgaaagc
     9661 tccataaaac gcttggtgtc tgaaacgaca tcagggtcac tcagcaattc tcctagttct
     9721 tcataacggt cttctacagc ttgtagttga tcatagatgt tcattttttc tccttatttc
     9781 tcaattgtta aatcatagat tgctactact tcattctcgg atatttcccc agtttcttta
     9841 aatccataac tgagataaca aaatcttgcc tgttcatttt ctggttcata agacaaccaa
     9901 agtttattgc ttaaacctgc tggcgctgtt cgaacatagt ctagtacttt atccataatt
     9961 ggtttaaaat atccttgatt ttgaaaattc ttatcaatca taaaacgaaa tagtaaataa
    10021 tttccactac taattacgat ctttttatca taagctatca tcacaaaacc tataattaca
    10081 tcattatcat aaactgccaa tggagctaca aaatctccat ttttagtgta gacgtatgct
    10141 tcagctaaac taattgcgtt ggttgcaatg aattgttttt gatattcctt gacatccaaa
    10201 tttaaaacat caaaataatt ttccattgta acatctctta gttcaattgt catagttttg
    10261 ctccttgtta gaggttatca ttggcgcaaa ataatgttta cggcaaaccg aaatataggt
    10321 ttcattacca ccaatctgga tctgttctcc atcgtaaacg ggcagtccat cctgtgttcg
    10381 caacaccatg gtcgcctttt tcttgcaata ctgacagatg gtcttgattt cgtcaatctt
    10441 gtctgctaaa agcaagagat atttggaacc ttcgaacaat tcattgcgaa agtcattttt
    10501 caagccaaaa gccatgacgg gtatgtctaa ctcgtccaca acacgagcta ggtcgtaaac
    10561 atggtggcgt ttgagaaact gggcttcatc gaccaaaaca cagtaaggtt tttctggtag
    10621 gtctcggata tagccaaaga tatccgttgt ttcctcaatc gtaagggcag ggcgtttcat
    10681 gccaattcga ctcgacacat agccaacacc gtcacgcgta tccagagccg aagtcataat
    10741 gacaacacct tttccttgct cctcgtagtt ataggccact ttgagaatct caatcgtttt
    10801 accagagttc atggtcccat aacgataata caactgtgcc atgtttcttg cttcacgtcc
    10861 atttctaaat ttttgctaca ttctagtata tcataatttt cttaagcttt aaacggcaaa
    10921 atgtggtaaa atggaagaaa tcaaaactag tggaggaagc tattatgcca tttgtacgca
    10981 tcgatttatt tgaaggacgc acgctcgagc aaaagaaagc tcttgctaag gaagtaacgg
    11041 aagcagttgt ccgcaacact ggagcccctc aatctgctgt ccatgtcatc atcaacgaca
    11101 tgccagaagg aacttacttc ccacaagggg aaatgcgtac taaataagct agcttaagca
    11161 gaattgctta ggctttttca atctccaagt agcattcatt gaagaaatat cctaaatttg
    11221 ttacaatttg aaaagaaact tggagaattt ccaagaaaat agctattaat taaaggaaac
    11281 attatgatta cacgtgaatt tgataccatc gctgctatct ctactccact aggtgaaggg
    11341 gctattggta ttgtccgcct gagcggaaca gacagttttg ctattgcgca aaagattttt
    11401 aaaggaaaag acttgaacaa ggttgccagc cacactctca actacggtca cattattgat
    11461 cctctgactg gtaaagtcat ggacgaggtc atggtaggag ccatgaaatc tccaaaaacc
    11521 ttcactcgtg aagatattat cgagattaac acccacggtg ggattgcggt gaccaatgag
    11581 attctccagc tagctatccg tgaaggagct cgattggctg aacctggtga atttaccaaa
    11641 cgtgccttcc taaacggtcg cgtagatttg acacaggctg aggcagtgat ggacatcatc
    11701 cgtgccaaga ctgacaaggc catgaacatt gcagttaagc aattagacgg ctccctttct
    11761 gacctcatta acaatacccg tcaagaaatc ctcaatacac ttgcccaagt tgaggtcaat
    11821 atcgactatc ctgaatatga tgatgttgag gaagctacta ctgctgttgt ccgtgagaag
    11881 actatggagt ttgagcaatt gctaaccaag ctccttagga cagcccgtcg cggcaaaatc
    11941 cttcgtgaag gaatttcaac ggctatcatt ggacgtccca acgttgggaa atcaagcctt
    12001 ctcaacaacc tcttgcgtga ggacaaggct atcgtaacag atatcgctgg gacaacacga
    12061 gatgtcatcg aagagtacgt caacatcaat ggtgtacctc tcaaattgat tgatacagcc
    12121 ggtattcgtg aaacagatga catagtggaa caaatcggtg ttgagcgttc gaaaaaagcc
    12181 ctcaaggaag ccgacttggt tctactagtg ctaaatgcca gtgaaccact gactgcgcaa
    12241 gacagacaac ttcttgaaat tagccaagat accaatcgca ttattctact taataaaacc
    12301 gacctgccag aaacgattga aacttcgaaa ctacctgaag aggttatccg tatttcagtc
    12361 cttaaaaacc aaaacatcga caagattgaa gagcgaatca acaacctctt ctttgaaaat
    12421 gctggcttgg tcgagcaaga tgctacctac ttgtcaaacg cccgtcacat ttccctgatt
    12481 gaaaaagcag ttgaaagcct acaagccgtt aatcaaggtc ttgagctggg gatgccagtt
    12541 gatttgcttc aagttgactt gactcgtact tgggaaatcc tcggagaaat cactggggat
    12601 gctgctccag atgaacttat tacccaactc tttagccaat tctgtttagg aaaataagaa
    12661 aaatccatga tccttcattc ggtcatggat tttattgtct ttattagtaa tctggtctta
    12721 agacccctgt tacagttgcc ttagttgctt cgtagtcgcc atctacgaca accttgataa
    12781 tgcgtttggc atcttcttct ggtgctggaa caagaggtag acgagtgggt ccagcttcaa
    12841 atcccatata gttaagaact gccttaactg gagcaggact tggataagag aagagagcat
    12901 taaccttagg aatgaattta cgctgaattg ctgcggcttt cttcatatcg ctttctgcaa
    12961 tggcagtaaa catctcgtgc atttcatccc catttgtatg agaggcaaca gaaataaccc
    13021 catccgcccc aaggttcatg gcatggaaag catctccatc ctcacctgta taaatcaaga
    13081 actcttcagg cttgtgctca atcaaataag ccatattagc caagctagta cattctttga
    13141 caccgataat atttggatag tcagccaagc gaagcatggt ttctggagtc aattcgacaa
    13201 ctacacgccc tggaatgtta tagataataa ttggtaggtc agaagcatct gcaatagcct
    13261 taaagtgctg atacatccct tcttgagaag gtttgttgta gtaaggaaca atagcaagcc
    13321 cagctgcgaa accaccaaat tccgctactt ctttgacaaa ctcaatagag tcacgcgtat
    13381 cattagtacc tacacccgca atcaaaggaa cgcgtccatt gacaaccttt tgtacagccg
    13441 caaacaactc caactcctca tcgtgggtca aagttggact ctcagcagtc gttcctgcga
    13501 gaagaattcc atccgtatga tgggccaata aatgctcaat caaggctgga atagcatcaa
    13561 agttaatgga accatcctca tggaaggggg taataaaggc tgtaatgatt ttacattctt
    13621 ttaaatcttg ataagacacg aaaacacctc tctatttcaa agaaggagtt catctgaact
    13681 cctaaacgat atgactattt taattcaaat ttcaattcgg ctgttggacg aaccaatcca
    13741 cgttcatgaa gagtttcagc aatctgaact gagttccaag cagcaccttt gagaaggtta
    13801 tctgaaacaa cccacatgtg aattcctttt tctgcatcca agtctttacg gatacgacca
    13861 acaaaggtat cacgcgaacc aactgcattg atggcttgag gatagatttg atgagctaca
    13921 tcatcttcaa gaacagcacc tgggaaggct gcgatagctg cttttacttc ttcgattgga
    13981 gccacttctt ttgtttcgat ataaacagac tcagagtgag ctgacaagac tggaatacgc
    14041 acacatgttg cagatactgc aatgctatca tcttccataa ttttcttagt ttccttggtc
    14101 atcttcatct cttcgtacgt gtaatcatta tcagtgaaaa catcaatttg tggaagagcg
    14161 ttaaaggcga taggataatg tttcttgtca ccacctgaag gcaaaatttc cgcatgcaaa
    14221 tcacatggtt tcacaccatc attcaagact tcacgaagtt cacgttgtgt ctcaagaatt
    14281 gctcccatac cagcacctga aacggcttga taagttgaaa caatgatacg gtccaagccc
    14341 catttttggc gaaccggctc aagagccacc atcatttgga ttgttgaaca attagggcag
    14401 gcaatgattc cgttgtgagc atcaagtgca tgagcattga cctctggaac aaccaaagga
    14461 acatctggat tttgacggaa ataagatgta ttatctacta ctaccacgcc agcttttact
    14521 gcgtatggtg catacttagc tgatgtagaa ccacctgctg aaaagagagc aatatcaact
    14581 ccttcaaaag ctgtttcagt cgtttcttca atcgtaatat cttgatcttt aaatttcaat
    14641 gacttacctg ctgaacgtgc agaagcaagg taacggattt tatcgattgg aagtgttgat
    14701 tcttccaaca tttttatcat ctgagcaccg acagcacctg tcgcgccgac tacagcaact
    14761 gtatatccca taaataacct ctttcggaat tttctaaaaa tttctataat agaagtatta
    14821 tactactttt tccatgaatt gcaaagcttt ttcatcattt tttggaaaat aaaaatcccc
    14881 aatcgctaga ctagggatta agaggcatct tcatgtgatg agcaggttca cacaactcat
    14941 caaggtccgc tcctgcgtta tgacctcctt atgctcaata gtagcccgaa gactacctat
    15001 cgactcatcg catctactat tctactaaag agaatgactt ttgtcaacag acgaaactct
    15061 ttatactctt cgaaaatctc ttcaaacttc gtcagcgtcg ccttgccgta tgtatggtta
    15121 ctgacttcgt cagtttcatc tacaacctca aaaacatgtt ttgagctgac ttcgtcagtt
    15181 ctatctacaa cctcaaagca gtgctttgag caacctgcgg ctagtttcct agtttactct
    15241 ttgattttca ttgagtatta gttttattta cacaggtgca taagaaatat attggaaaaa
    15301 ttgggggcag acataacaaa aacccttgta aatcaagggc ttttctgtct atttaatgct
    15361 aattgccggg attgaaccgg cgacctcatc cttaccatgg atgcgctctg ccaactgagc
    15421 taaatcagct tacttaagaa gtatactata atcagaaaaa cttgtcaagt ttccttagaa
    15481 attttccata ggaaaaagcc aggtaagaac tacctagctt atcttcttct atttacttaa
    15541 atcaattcga cgaccaattt taccaataat aatcgctcca ataatcaatg aagcaaattc
    15601 accgattcct gttgtaaacc aagtaaggaa gaatggtaat tctgctacaa tatttaactc
    15661 tgcagcgata gtaaacatgg aaattgaaaa gaggattgaa aagaagaaat gatcttttcg
    15721 aatcaatccg ttaaagaggt aatccttgct atattttgca aaaagccaaa cacctaaact
    15781 gaggaatact aaggttgaac caccaccaac aaagacatct agcagtccga agctaaagaa
    15841 attagcaatc atacaaccga tggtaactcc gatgatgtac ttaggattgt aaaaagccat
    15901 aaagttcatc atttctgaaa tacgaaactg ataagcacca tagctgatgg catttagggg
    15961 cggtgtgaca gtcaaaacga cgtagatagc agcgacgatt gcaatatctg caacatcacg
    16021 aatagttaat tttttcatct tttctccttt ggcggttatc cgcgtgtaat atgcttggtg
    16081 aaagaagcta agcaccaagg gttgattcat tcaaccttac tagtatagca caatagcctg
    16141 ctttatgcta tacttaaagc atgaaaaaac ctattcaaaa attttttaac aatgaaatct
    16201 tagcctatct cttttttggc cttgcgacaa ctctagtttc gattctctca cgactagtca
    16261 tttatcaact aagtcatcag gaactccttg ctacggcctt ggcaaatatt atcggtatct
    16321 tatttgcctt tatcacaaat gatacgattg tatttaagca aaaaaagaaa aatagactta
    16381 tccgtttagt gaaattttct cttgctcgcc tttctacttt tctgttagac ttgtttttaa
    16441 cttttctctt tgtaacccaa tttcctcaca tcatagggca attcgtaact gaaaacattg
    16501 ataagataaa tgctattgaa acaataattg cacaaatatt cattattatc atcaattata
    16561 tcttgagcaa ggtctatatt tttagaaaat aatgctccat ttatgagcct tttaattgcc
    16621 ttctataatg atttaggata caatgaaaca cgaaacattg aaaggaaaac agtatgttaa
    16681 aaaatctaaa atcgttcttg cttcgaggaa atgttattga ccttgctgtc ggtgttgtca
    16741 ttgcctctgc ttttggtgct atcgttactt cacttgtaaa cgacattatc actcctctta
    16801 ttttaaatcc cgctttgaaa gctgctaaag tcgaacgtat cgctcaactt tcttggcatg
    16861 gagtcggcta tggtaacttt ttaagtgcta ttatcaattt tatctttgtg ggtaccgccc
    16921 tcttctttat tatcaagggc attgaaaaag cacagaaact gactggcata aagaaagaaa
    16981 aaactgccga aaaaaaacca accgaattgg aagtccttca agaaataaaa gctctccttg
    17041 agaaaaaata atcgattaaa aggatctagc gcaaagctaa atcctttttt ctttactctt
    17101 tgggtaactt gcctgctttt tcaagcattt ttttgataag ataaggcatc ttaacattct
    17161 cccgcccttt tttctcaatc agttttttca caaattctgg catttgtaaa tcttcagatt
    17221 cagctaaaat gtttttcgtc tcgtctgaag gaactaactc atcaaaggtc tcttcttctg
    17281 ggagaaattc cttaaactct tgattttcat tgactctaac tgtattgacc aaagcatcaa
    17341 tcaaacgaga atctgtattt ggcattggtg gacgatggta gtttacccca aattcctggc
    17401 ataagtcata acattctaca tcattgtcaa acaagacctc aatatgctca ctgatgaaac
    17461 taataggtac aaaaatataa tggtctggat gttctgtctg ttctctgaga tactccaaaa
    17521 catctggttt aatccatgga ataccaatat cactctcact ctgccaagtg ttggtatatt
    17581 gttctgaact caagcccagt ttttcagcga ctagcttgct attttcgaaa atttggtcta
    17641 tataaggatc accaaagtcc aaggcaaaaa tgggcacact atgggctgaa aagatgactt
    17701 taaagctgtc ttgctttact tcttctttta aaatcttagc aatttcatct gcccaatagt
    17761 ttaagagcac ttcttcctga taccagtcct taatgaccaa aaattgaatt tgtttgcttt
    17821 ctaaaaattt ttcatagccc atgacagagt agaaagaata atggggctcc aaaatcaagc
    17881 agatacattc ttcaacacca tcagtttcca tctgcttgat cacgtctgga ataaagggac
    17941 gagaaaattt gttggcaaag tagataccgt actcctcccc taaacgttct ttgactaaat
    18001 tgacctcctc acgtgtaatc cgctgcaaag gcgtcccacc gatacgaata tagttgtcat
    18061 agagagtctg gatttcgtgg tcttggggtc taactccacg acgaatgttt gtaaagaatt
    18121 ctgctactcc ttcaaaacta atctcctctg gagatccaaa ggtcatcatt aatatggctt
    18181 ttttcatgtt atcatcctta tgatattatt ttctctatcc attctatcat gaaagaaaaa
    18241 aatagtaaac gtttcctacg tctactattt cttttgctct tttgtttttt tatctcgtct
    18301 tataattagg atgataagga caagaagtaa gaggacgata ataaggagta gaaaggctag
    18361 aatgtaaatc caatgactgg gaattccgtg atcgaaatac cgtgttccta ctgcttcccc
    18421 actttcatcc tgtgctactt tcaccggtgc atccgttatt ttagctaaaa ggatatgtcg
    18481 tccattagtt atgttaagga aacatctcgc ttgtacttgg ctttacttag cagatattga
    18541 tagtaagcct ggtgttcctc atccttggaa ctcaaggttc gataaatctc cgtgtcatag
    18601 gcatccactt ctaaaatccc aaaaatttca agaccacgtt ctcgaccatt gtagtagata
    18661 gaaccgtagc gatgctggtc gaagaattcc ttatcagcaa atttcttaat ctcaccgaac
    18721 atagcccctg aagccataga gtgtccgtag atgatcgtat taaaatcagt aaagttggga
    18781 ttaaagcgat agtcgagaaa aattgatccc attatcgtat aatcgccaaa ggcattgcgg
    18841 tcaaggtatt tgacattatc ctgactttgt agaagcggat agtctacttt ggtcccatca
    18901 aggctaatcc aaccgcaaac atctttgttc aagtgctgta gggtttcaaa acctgtgctc
    18961 gaagcatcct tttcaatagg attatagtga tcatagtcac ttgcttctac tactctctgc
    19021 caactaaaaa gagctacaaa aacacagaag aataccagaa atacgttttt aaatcgcgct
    19081 cttttttgaa ccgtcatcag cactcctttc tatgcgagat ttcttaaaag gaaaggaggc
    19141 gggacaaaac cgcctctttt ctattcaccc ttagtcagta aaagggtctg cttcttgcat
    19201 tttttttact ctctcttcta cgacgtagga tcaagtagag gacaaggagt aagaaaatca
    19261 agagcaagaa caatacaaac aagataaggt accaaatcca tagtgggatt gatgagagga
    19321 aacgtccaag agacgagtca tcaaagacac tgtatggaaa tggttttgat ttttttgtat
    19381 ggaaagtatt tttagggacg gtgtctgtaa tctttgcgac tacgatatga cgaccattgg
    19441 tcacatcgag aaaacaggta ctgagtagga tgatacgatc actcggtgag agtgagatat
    19501 cccgcttgtg catggcgact gagagcaggt ggtctagata ggcctgctgg cggtcctcac
    19561 cctgtattcc tggatcatag atgttaaagt cataggcatc aacctccaac atctcaaaga
    19621 tctcgagccc tttttccaca ccattgtagt atatactacc gtaacgatgc tggtcaaaaa
    19681 attcctgatc agcaaactta gccacatcac caaacatgac cccattttct acgtggtgcc
    19741 cgtagataat ggtattaaag tcttcgaact taggattatt tcgtgcatcg agaaagatag
    19801 cgcctgtcgc tgcaaactca cctttggagt ccttgttgag atacttttca ttgtccttgg
    19861 cttggactaa gggatagtcg atattggtgc catagacatt gatccaaccg aggacttcgg
    19921 gattgagttc ttggagtttg ctgaagcctg aaaaactggc cagctcatcc tgttgcgtcg
    19981 tgacaggtcg ataagcttcg tactcacttg acgaagctaa ggagtagact tggttggaat
    20041 cccaaagggc gtagaaacca aagagaaaga tcaaagctgc aaaaatgagc agcaagtgat
    20101 tgatcacccc atccacagca agaacgagac gcctagacag actctttttt ggtttctcac
    20161 gcttggtttt catcatctgc ccccagccgt ttaggcttct ctacgttttt tagacaagac
    20221 aacaaatcca gccaaaccaa gaccaatcat caaaatgaat ggaaggttat caatcaagag
    20281 accagtagga gtaacgtcgt cgattttgtt agtaactttg ttgtcgtttt tcttttcacc
    20341 tatgaggata tctgttaatg taaaatctac atttttttgt ccttgagatg tttgttctgc
    20401 accatttgta gtataaattg atgaatctgt ataacctgct actccagttt cagttactgt
    20461 atatttagaa cctgctggta gttgttcaac tgtaaatgtt tcaccatctt taagagtaaa
    20521 attgtactca actgtatctg ctggggtcac tactgccaac acctctgact tagatccatg
    20581 ttttactata atattcgtaa caggggtttc tgctgtttta tttgtacttg gaagttgaat
    20641 cttgatatgg aattggaaat ctcttgtttt atcccctgaa gctccatcaa cttttttagt
    20701 aattgttaat gctttcgcat tggggtctac ttgactcgga tcatcaggat taggctcctt
    20761 acttgcgtct ttggtatatg tgttatcaaa taaattataa acgccttgtt cacttggttc
    20821 tactttaggg gcgttagatt tattattttc cttaaagtat actgatgaaa tatagacacc
    20881 accatttact ttattcttaa caataacgtg catttcatat gtacgtttgt cgtatctcat
    20941 ggcatcaatt gttttaccat ttttggtaat cgcctgccat ccagtttttg cttccgctac
    21001 atcataaacg tattcaccag catggccata agcaactccc gaaaaaatgt cttctgttgc
    21061 tttttgaatc ttatcagcag ttgggtgatc atttttattg tattctacat ttctatctgg
    21121 aattgcaact ccggtttgat atggcgcacc gttagaagat tgtcctgttt tttctgtaaa
    21181 cttaaatgta aatgtcgctg ttggtgttgt tattccctct gcaatattca acgttttatt
    21241 aatcttcaca acagcattat tttcaccatt tgctttggtt attgctgaat tatcatccac
    21301 aaaagtcggg acacctctga atacggacaa agcggttata gctgcaatgc ttgcagtgaa
    21361 tagcttttta aagaatgttt ttttcatgat tatttctttt gtttttttat tttctttttt
    21421 cattatgatc atcaaaagcc tcgtcttcta atgactgtta caactttgcc ttgagtatcc
    21481 tgtataggga tggttccaaa agcacgactg tctacagccg ttgttcgatt gtccccgagg
    21541 acaaaaagtt gtcctgctgg gactttcatc gggaaggttg ccccttcctt atagagcagc
    21601 gtttctttgt agatatcttg ctcttgttga ggagaaccgt tgatgatgag accattttcg
    21661 tttatatcga ttgtacttcc ttcggttgct atgacacgcg ccactctttc cttaccttta
    21721 taactataga ctagcaaatc accaatcgaa tagcgtttat ccaacctata atagacgacc
    21781 aagtcgccat cttttaaggc gggcttcatg ccatcgtcat tgtatcttaa taatccaaag
    21841 acgaaaaagt aaaggatggc tagtacaaat ccaaccatca gaagtttact gactaagtaa
    21901 aggatatctc cccagacact cggcggagac ttatcccgat tgacttgtgt tactggtttc
    21961 ttatgtttct ttttaagcag catcacttcc tcctattcac cacaaggtga ccaagtaaga
    22021 tgataaagaa gatccctcct agaggaagaa cgactccaag cacgagagtg ctactcaaga
    22081 agtcaattcc cgtttcaggt acgctgtttt tattattcgt gactgtcgca gacttatttg
    22141 catcaagagt tccttctttt ttatcatagg acacctgata cccacgagac gaacttgctt
    22201 cttcttcgat actataacga gcatttagag gaaggtcgag aatcttgatg gttttatctt
    22261 tctttagatc aactgtcgcc ttaccattgg tgaattgtag cgttgttttt tgattattta
    22321 ctattgcact atacgatcca ttcaatggtt tattttgcgc atcctttacg ttaatggtaa
    22381 tcttaaagga ttgcagaaga ttagcaaaag cacctgtaac tttttttgtg actgaaagtg
    22441 tcggaagtct gtggttggtg acgattgttt gaacatcctc tgacaaagtc ccctcttgtt
    22501 tttcataggt gacatgatat cccgttctag agttctctac ttcttccacc ttatagtggg
    22561 tatcacgagg cagataactg agggcagttt cctgactatt tttgagagaa atctgtgctt
    22621 tcccgttagt cactgttgta ttcccaaact tgccgttaat cggcttgcca tccttatctg
    22681 aaaaggtaac attaattgtg aaagatttat taggtgattg ttcaccttct aggattttct
    22741 ttacggtgag acttggaagc tgtcggtaaa taactttgaa ggatggtttc tctttcgtac
    22801 ctgtcttttg aacttcgact gtgtatcccg gagcgtctgg tgctttcagt tcgtagtcat
    22861 atcccttagc tacctttgca aaactagtat tttcagtaaa actattcgtt tgaactggac
    22921 tagtgacctt tcgataggaa tcctttatac ttccatcttt cttttggttt aagtttaatt
    22981 cgacactagg tggacgattt gctgatggat ttgattttcc atccacacct ttccactcaa
    23041 tctctacagg aaccgtcaat ggatctgacg gcttgaaagt gggcttctca gaatagtctt
    23101 ttattttggt acgtgaggtc ccaataccat aggaataggc aacttttgcc cccttgttgg
    23161 agtagagttt agtagcatcg gcttcttcgg catcatcact agtaagtgtc ttatcacccg
    23221 caatcgcatc aaatgcctct tggctagagg tgacagtaaa cttgagaacg tacttggcgt
    23281 catcctccaa agtgtaactc ggattaaact tggctgttac ttccactaat ccctgttcgt
    23341 tcttcttagt ttcaatcgta acttgattgt cattcagttc ttgctcgtta ccatccttga
    23401 ttttgacgac acgaaggtgt gaagcatcac caggtaacag ctgcacgtac ttggacaaga
    23461 catctgatat agttacatgg tgtacgccta gaggtagaat tttatctgtg atttctttga
    23521 atttttgttg caagtcattt tcattattgg cagaaaatat ttcgttgggg attccagaat
    23581 tataaccact gatatatcct ttaagagatg tgatactatc gtttgaacct atatatctga
    23641 atttgattga ataaaagcca tctatttttg gagctattaa actagatata ttcagcattc
    23701 ctctctctaa accagaggta aaccaatatg gagtatcaat catacgtccc acatttggat
    23761 tagaataata gttatatatt gttctcccac tattagactc gtaatacata ttagcttcgc
    23821 catctgacag cagaataact actttttttg ccccagccct tgcactttgc aacaatcttt
    23881 gagcttcagt taacccagca tttatatttg tcccagtgcc aatgcccgcg tccataccat
    23941 aagaacgact agagtcagca attgttaacg aactaacaat attttttgag ctattagaat
    24001 ccgtgctcca agatactaaa gttttagcat catcatatga agagatattt gaataccaac
    24061 cagctcaatt tggccaagtt ccatattgat aactcagata atattcatta taataacggt
    24121 cattatactg attatcaatc tttccggcaa aaccaactat agacagacgg ttttgggaat
    24181 tagaaagaat tgtatcaatg agcccctgac gaccattcac acctttcagt gtatgtttta
    24241 gtgcttcaat cctactaatg tatcgtctac tagagtaaga ccacacatct cgctcttcca
    24301 tactccctga aagatcggca accaagacta catccaaggg ttctgactgg gtctccgttc
    24361 ccagtttaga tgtgatatcc agtgacaact catactggtc tttttgccct gatatcggag
    24421 taatcgtttt gtgcagagtt gtctgaggtt cagttattga atctgcacgt aaagaattaa
    24481 ttggtgcaca caaaaaaaga atagcgagaa agataactaa actactggta aaatattgaa
    24541 tcttcttaaa atcatattga acgttcatgc tatctccctt gctagaatat gttttaatct
    24601 gatactatat aaatctgaga cttc
//
LOCUS       DAVKJJ010000032        24198 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000032 DAVKJJ010000000
VERSION     DAVKJJ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24198
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.032"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            651..2804
                     /locus_tag="VW144_001313"
     CDS             651..2804
                     /locus_tag="VW144_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU9600758.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFAEEWTGVTLFMAPSPDYKPHKEQKNGLFSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVVSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGSVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2817..4166
                     /locus_tag="VW144_001314"
     CDS             2817..4166
                     /locus_tag="VW144_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU9600759.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLINQTEAKIRDYQTAKSAMETGASLASQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4336..5043
                     /locus_tag="VW144_001315"
     CDS             4336..5043
                     /locus_tag="VW144_001315"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU9600760.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            5100..8825
                     /locus_tag="VW144_001316"
     CDS             5100..8825
                     /locus_tag="VW144_001316"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEU9600761.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNVGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDAFVA
                     ECNKENIDAVVVAIVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEAIQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELETALTAQLGGFVFTSPEEIAGVEKVG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            8918..10360
                     /locus_tag="VW144_001317"
     CDS             8918..10360
                     /locus_tag="VW144_001317"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU9600762.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEDRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSMTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10397..11419
                     /locus_tag="VW144_001318"
     CDS             10397..11419
                     /locus_tag="VW144_001318"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU9600763.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLVSSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11416..11961
                     /gene="purN"
                     /locus_tag="VW144_001319"
     CDS             11416..11961
                     /gene="purN"
                     /locus_tag="VW144_001319"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU9600764.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            12045..12554
                     /locus_tag="VW144_001320"
     CDS             12045..12554
                     /locus_tag="VW144_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9600765.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12579..14126
                     /gene="purH"
                     /locus_tag="VW144_001321"
     CDS             12579..14126
                     /gene="purH"
                     /locus_tag="VW144_001321"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU9600766.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDMTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELSAN
                     GETTYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDRPTVVALKHM
                     NPCGIGQADNIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLNGAVLASDSFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14248..15510
                     /gene="purD"
                     /locus_tag="VW144_001322"
     CDS             14248..15510
                     /gene="purD"
                     /locus_tag="VW144_001322"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU9600767.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVSETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELYQQKIEGLFYRTDIGSKAIK"
     gene            15913..16401
                     /gene="purE"
                     /locus_tag="VW144_001323"
     CDS             15913..16401
                     /gene="purE"
                     /locus_tag="VW144_001323"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU9600768.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16388..17479
                     /gene="purK"
                     /locus_tag="VW144_001324"
     CDS             16388..17479
                     /gene="purK"
                     /locus_tag="VW144_001324"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU9600769.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17489..17716
                     /locus_tag="VW144_001325"
     CDS             17489..17716
                     /locus_tag="VW144_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEU9600770.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVKAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            17779..19077
                     /gene="purB"
                     /locus_tag="VW144_001326"
     CDS             17779..19077
                     /gene="purB"
                     /locus_tag="VW144_001326"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU9600771.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNLVYYTKRVDDIFERLGLGD"
     gene            complement(19132..23070)
                     /gene="strH"
                     /locus_tag="VW144_001327"
     CDS             complement(19132..23070)
                     /gene="strH"
                     /locus_tag="VW144_001327"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9600772.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAVLQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEALKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
     gene            complement(23385..24101)
                     /locus_tag="VW144_001328"
     CDS             complement(23385..24101)
                     /locus_tag="VW144_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9600773.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
BASE COUNT         6608 a         4781 c         5754 g         7055 t
ORIGIN      
        1 ttaattagaa aaaaaatttt taaagtcttc ggagaagaaa acagaaaaat ctgttaaaaa
       61 ataatcaaaa ctataaatga tgaatctgaa tcaaaattat tttgcgcatg taaagaggag
      121 tcttatagta acgagtcaaa aaaggagtaa ctatgaatcg taatttagaa cggtgttatc
      181 tattctgact agggatagat cataccatag gtagcttaga aatagcagag acattagaaa
      241 ttgaagtaat aaataggatg tcgtaagtgt tactatcaat gatttatttg tttcaagctt
      301 gcctagggtg tcagtaaaaa atcattttcc tttcaatagc atatttttag tgggcaggac
      361 tcttgttctg cctatttttt tatccaaaaa gtgcagttgg gagggagata ggctcatttg
      421 ggaaggaagt ccagtttttg tttagtgatt ggggtaagat agttgttatc agatgagttt
      481 atattcttcg aaaatcaaat tcaaaccacg tcaacgtcgc cttgccgtat atatgtgact
      541 gacttcgtca gtcctatcta caacctcaaa acagtgtttt gagcagcctg cggctagttt
      601 cctagtttgc tctttgattt tcattgagta ttagggaaaa ggagatgaat atgaaatttg
      661 ggaaacgtca ctatcgtccg caagtggatc agatggactg cggtgtagct tcattagcca
      721 tggtttttgg ctactatggt agttattatt ttttggctca cttgcgagaa ttggctaaga
      781 cgaccatgga tggaacgacg gctttgggct tggtcaaggt ggcagaggag attggttttg
      841 agacgcgagc cattaaggcg gatatgacgc tttttgactt gccggatttg acttttcctt
      901 ttgttgccca tgtgcttaag gaagggaaat tgctccacta ctatgtggtg actgggcagg
      961 ataaggatag cattcatatt gccgatccag atcccggggt gaagttgacc aaactgccac
     1021 gtgagcgttt tgcggaagaa tggacaggag tgactctttt tatggcacct agtccagact
     1081 acaagcctca taaggaacaa aaaaatggtc tgttctcttt tatccctata ttagtgaagc
     1141 agcgtggctt gattgccaat atcgttttgg caacactctt ggtaaccgtg attaacattg
     1201 tgggttctta ttatctgcag tctatcattg atacctatgt gccagatcag atgcgttcga
     1261 cactagggat tatttctatt gggctagtca tcgtctacat cctccagcaa atcttgtctt
     1321 acgctcagga gtatctcttg cttgttttgg ggcaacgctt gtcgattgac gtgattttgt
     1381 cctatatcaa gcatgttttt cacctcccta tgtccttctt tgcgacacgc aggacagggg
     1441 aaatcgtgtc tcgttttaca gatgctaaca gtatcatcga tgcgctggct tcgaccatcc
     1501 tttcgatttt cctagatgtg tcaacggttg tcattatttc ccttgttcta ttttcacaaa
     1561 ataccaatct ctttttcatg actttattgg cgcttcctat ctacacagtg attatctttg
     1621 cctttatgaa gccgtttgaa aagatgaatc gggataccat ggaagccaat gcggttgtgt
     1681 cttcttctat cattgaggac atcaacggta ttgagactat caagtccttg accagtgaaa
     1741 gtcagcgtta ccaaaaaatt gacaaggaat ttgtggatta tctgaagaaa tcctttacct
     1801 atagtcgagc agagagtcag caaaaggctc tgaaaaaggt tgcccatctc ttgcttaatg
     1861 tcggcattct ctggatgggg gctgttctgg tcatggatgg caagatgagt ttggggcagt
     1921 tgattaccta taataccttg ctggtttact ttaccaatcc tttggaaaat atcatcaatc
     1981 tgcaaaccaa gcttcagaca gcgcaggttg ccaataaccg tctaaatgaa gtgtatctag
     2041 tagcttctga gtttgaggag aagaaaacag ttgaggattt gagcttgatg aagggagata
     2101 tgaccttcaa gcaggttcat tacaagtatg gctatggtcg agacgtcttg tcggatatca
     2161 atttaaccgt tccccaaggg tctaaggtgg cttttgtggg gatttcaggg tcaggtaaga
     2221 cgactttggc caagatgatg gttaattttt acgacccaag tcaaggggag attagtctgg
     2281 gtagtgtcaa tctcaatcag attgataaaa aagccctgcg ccagtacata aactatctgc
     2341 ctcaacagcc ctatgtcttt aacggaacga ttttggagaa tcttcttttg ggagccaagg
     2401 aggggacgac acaggaagat atcttacggg cggtcgaatt ggcagagatt cgagaggata
     2461 tcgagcgcat gccactgaat tatcagacag aattgacttc ggatggggca gggatttcag
     2521 gtggtcaacg tcagagaatc gctttggcgc gtgctctctt gacagatgcg ccggtcttga
     2581 ttttggatga ggcgactagc agtttggata ttttgacaga gaagcggatt gtcgataatc
     2641 tcatggcttt ggacaagacc ttgattttca ttgctcaccg cttgactatt gctgagcgga
     2701 cagagaaggt ggttgtcttg gatcagggca agattgtcga agaaggaaag catgctgatt
     2761 tgcttgcaca gggtggcttt tacgcccatt tggtcaatag ctagaaagag gagaggatga
     2821 aaccagaatt tttagaaagt gcggagtttt ataatcgtcg ttaccataat ttttccagta
     2881 gtgtgattgt acccatggcc cttctgctcg tgtttttact tggctttgca actgttgcag
     2941 aaaaggagat gagcttgtcc actagagcta ctgtcgaacc tagtcgtatc cttgcaaata
     3001 tccagtcaac tagcaacaat cgtattcttg tcaatcattt ggaagaaaat aagctggtta
     3061 agaaggggga tcttttggtt caataccaag aaggggcaga gggtgtccaa gcggagtcct
     3121 atgccagtca gttggacatg ctaaaggatc aaaaaaagca attggagtat ctgcaaaaga
     3181 gcctgcaaga aggggagaac cactttccag aggaggataa gtttggctac caagccacct
     3241 ttcgcgacta catcagtcaa gcaggcagtc ttagggctag tacatcgcaa caaaatgaga
     3301 ccatcgcgtc ccagaatgca gcagctagcc aaacccaagc cgaaatcggc aacctcatca
     3361 atcaaacaga ggctaaaatt cgcgattacc agacagctaa gtcagctatg gaaacaggtg
     3421 cttccttggc cagtcagaat ctagcctact ctctttacca gtcctacaag tctcagggcg
     3481 aggaaaatcc ccaaactaag gttcaggcag ttgcacaggt tgaagcacag atttctcagt
     3541 tagaatctag tcttgctact taccgtgtcc agtatgcagg ttcaggtacc cagcaagcct
     3601 atgcgtcagg gttaagcagt caattggaat cccttaaatc ccaacacttg gcaaaggttg
     3661 gtcaggaatt gacccttcta gcccagaaaa tcttggaggc agagtcaggt aagaaggtac
     3721 agggaaatct tttagacaag gggaaaatta cggcaagtga ggatggggtg cttcatctta
     3781 atcctgagac cagtgattct agcatggttg cagaaggtgc cctactagcc caactttatc
     3841 catctttgga aagagaaggg aaagccaaac tcacagccta tctaagttca aaagatgtag
     3901 caagaatcaa ggtcggtgat tctgttcgct atactacgac tcatgatgcc gggaatcaac
     3961 tttttctaga ttctactatt acaagtattg atgcgacagc tactaagact gagaaaggga
     4021 atttctttaa aatcgaggcg gagactaatc taacttcgga gcaggctgaa aaacttaggt
     4081 acggggtgga aggccgtcta cagatgatta cgggcaagaa aagttaccta cgttattatt
     4141 tggatcaatt tttgaacaaa gagtaatgtt cgtgttttta gagttaaata atttttaaac
     4201 tgtgagaaag attcttcttg cagttttttc tttacaattt ttgaaaaaca tctgctattt
     4261 attcggttga attcttgtgt tttttggttt tttgtggtaa aatgtgctca agtaatacga
     4321 aaggcgaact ttaaaatgtc aaaacaattg atctattcgg gaaaagctaa agatatctat
     4381 acaactgagg atgaaaatct tattatttca acttacaagg accaggcgac tgctttcaac
     4441 ggtgtcaaga aggagcagat tgcaggtaag ggagtcttga ataatcagat ttcatctttt
     4501 atttttgaga aattaaatgc ggctggtgtg gcgactcact ttgtggagaa actttcagac
     4561 acggaacaac tcaataaaaa ggttaagatt attcctttgg aagtcgtgct ccgcaactat
     4621 actgctggtt ccttttcaaa acgttttggt gtggatgagg gaatcgcctt ggagactccg
     4681 attgtcgaat tttactacaa aaatgatgat ttggatgatc catttatcaa tgatgagcat
     4741 gtgaaattcc tacagattgc gggtgaccag cagattgcct acttgaagga agaaacgcgt
     4801 cgtatcaatg aactattgaa agtctggttt gctgagattg ggcttaaatt gattgacttt
     4861 aagctagagt tcggttttga caaggatggc aagattatct tggcagacga attttcacca
     4921 gataactgcc gcttgtggga cgctgatggc aaccacatgg ataaggatgt tttccgtaga
     4981 ggattgggag aactaaccga cgtttatgag attgtttggg aaaagttgca gggtttaaaa
     5041 taacaacctc aaggctgttt gggaatattg caagagctga aataaaggaa taagaattga
     5101 tggataaacg tatttttgtt gaaaaaaagg ctgattttca ggtcaagtca gagagtttgg
     5161 ttagagagct ccagcacaac gtgggactgt caagcttgaa aagtattcgt attgtgcaag
     5221 tatatgatgt atttgacttg gctgaggact tgtttgcacc tgcagagaag cacattttct
     5281 ctgagcaggg aaccgaccat gttttagatg aagtatctgt gcaggcggat cttgctaact
     5341 atgctttctt tgccattgaa agtctgccag ggcagtttga ccagcgtgca gcttcgtcac
     5401 aggaagcctt gcttttgttg ggaagttcga gtgacgtgac agtcaacaca gcccaacttt
     5461 acttggtgaa taaagatatt gatgcgactg agttggaagc tgtcaaaaac tacctgctca
     5521 atccagttga ttctcgtttc aaggatatca cgacagggat tgccaagcag gagttttcag
     5581 agtcagacaa gaccattcca aaattgactt tctttgaaag ctatacagca gaagactttg
     5641 ctcgctacaa ggccgagcaa gggatggcca tggaagtgga tgatttgctc tttatccaag
     5701 actactttaa gtcaatcggg cgcgtgccaa ctgagactga actcaaggtt ttggacactt
     5761 actggtctga ccactgccgt catacgactt ttgagacaga gttgaaacac atcgactttt
     5821 cagcttctaa atttcaaaag caattgcagt caacctatga caagtatatt gccatgcgcg
     5881 aggagttagg tcggtctgaa aaaccacaaa ccttgatgga tatggcgact attttcggtc
     5941 gttatgagcg tgctaacgga cgattggatg atatggaagt ctctgacgaa atcaatgcct
     6001 gctcagttga aattgaagtg gatgttgatg gtgtcaagga accttggctc ctcatgttta
     6061 aaaatgaaac ccacaaccat ccaacagaaa ttgagccatt tggtggagcg gctacctgta
     6121 tcggtggagc tattcgtgat ccgttgtcag gccgttccta tgtttaccaa gccatgcgta
     6181 tttcaggtgc tggtgatatt acagcaccga tttcggaaac tcgtgctggg aaattgccac
     6241 aacaagtcat ttctaaaaca gcagctcatg gttattcttc atatggtaac cagattgggc
     6301 ttgccacaac ctacgttcgt gaatacttcc acccaggctt tgtagctaaa cgtatggaac
     6361 ttggtgccgt tgttggtgcg gctcccaagg gcaatgttgt ccgtgaaaaa cctgaagcag
     6421 gtgatgtgat catccttctc ggaggcaaaa caggtcgtga tggtgtcggt ggtgcgacgg
     6481 gctcttctaa ggttcaaaca gttgagtctg tagagactgc tggtgctgag gttcaaaaag
     6541 gaaatgccat cgaagaacgc aagattcagc gcctcttccg taatggcaat gtcactcgtc
     6601 tgatcaagaa gtccaatgac tttggggcag gcggcgtctg tgtggctatc ggtgaattgg
     6661 cagacggtct tgaaatcgac ctcaacaagg tgcctcttaa ataccagggc ttgaatggta
     6721 cagaaattgc catctctgaa tcacaagaac ggatggcggt cgtggttcgt cctgaagatg
     6781 tggatgcctt cgttgccgaa tgtaacaaag aaaatattga tgctgttgtg gtggcgatag
     6841 taactgaaaa accaaatctt gtcatgcact ggaatggtga gacaatcgtt gacttggagc
     6901 gtcgtttcct tgacaccaat ggtgtgcgcg tggttgtcga tgccaaagtt gtggacaagg
     6961 atgtcaaact cccagaagag cgtcaaacat ctgctgaaac actggaatca gataccctta
     7021 cggttctatc tgacctcaac catgcaagtc aaaaaggatt acagactatc tttgactgct
     7081 ctgttggacg ctcaacggtt aatcacccac ttggtggtcg ttaccaactc acaccaactg
     7141 aggcatctgt gcagaaattg ccagttcaac acggtgtgac tcatactgcg tcggtcattg
     7201 ctcaaggttt caacccatat gtagctgaat ggtctccata ccacggtgct gcttatgcgg
     7261 ttatcgaagc aactgctcgt ttggtggctg ctggtgccaa ctggtccaag gctcgtttct
     7321 cttaccaaga gtatttcgag cgtatggata aacaagcaga gcgtttcggt cagccagtag
     7381 ctgctcttct aggttctatt gaagcacaaa ttcagcttgg cttgccatct atcggtggta
     7441 aggactccat gtctggtacc tttgaagaat tgaccgttcc gccaaccttg gttgcctttg
     7501 gggtgacgac ggcagatagc cgtaaggtgc tctctccaga atttaaagct gttggggaaa
     7561 atatctacta catcccaggt caagccctct ctgcagagat tgattttgac ttgattaaga
     7621 aaaattttgc tcagtttgaa gccatccaag ctgaccataa agtgacatct gcatcagctg
     7681 tcaaatacgg tggtgtagtt gaaagtttgg ctcttgctac ctttggaaac tatattggtg
     7741 cagaggtgac cttgcctgaa cttgaaacag ctttgacagc tcaattaggc ggctttgtct
     7801 tcacatctcc tgaagaaatt gctggagtag agaaggttgg acaaacgaaa gcagacttta
     7861 cactgactgt caacggtgtg aagctagatg gacacaagct tgacagtgca tttcaaggga
     7921 cattggaaga agtttaccca acagaattta cccaagcgaa agaactagaa gaagtaccag
     7981 ctgtggcatc agatgttgtg attaaagcca aagaaaaggt tgaaaaacct gtggtttaca
     8041 tcccagtctt tccaggaacc aactcagaat atgattcagc taaggccttc gaaaaagaag
     8101 gtgcagaggt caatttggtg ccattcgtga ccttgaatga agaagctatt gtcaagtcag
     8161 ttgaaactat ggttgacaat atcgacaaga ctaatattct cttctttgcc ggtggattct
     8221 cggctgcgga tgaaccagat ggttcagcta agtttatcgt caatatcctg cttaatgaaa
     8281 aagtgcgtgt ggctattgat agctttatcg cccgtggtgg cttgattatc ggtatttgta
     8341 atggattcca agccttagtc aaatcgggtc tcctacccta cggaaacttt gaagatgcta
     8401 acagtactag tccaaccctc ttctacaatg atgccaacca acacgtggcc aagatggtgg
     8461 aaactcgcat tgccaatacc aactcaccat ggttggctgg tgtgcaagtg ggcgatatcc
     8521 acgctattcc tgtttcgcac ggtgaaggaa agtttgtcgt gacggctgag gaatttgcag
     8581 agctccgtga caatggacaa attttcagcc aatacgttga ctttaacggt aaaccaagta
     8641 tggattctaa gtacaatccg aatggttctg tccatgccat cgaaggaatt accagcaaga
     8701 atggtcaaat catcggtaag atgggccact cagaacgtta tgaggatggt cttttccaaa
     8761 atatcccagg caataaagac caacacctgt tcgcatcagc ggttaaacat ttcactggaa
     8821 aataagactt acagattttc taatagatag tatcagtaat gtaaaagtca tgtaaatcta
     8881 gctcttgatg attacaaatg aaaattaggt ataaaaaatg acatacgaag taaaatctct
     8941 taatgaagaa tgtggtgttt tcggtatttg gggacatcca gatgctgcta agttgaccta
     9001 ttttggactc cacagtcttc aacaccgtgg tcaggagggg gcaggaatcc tctccaatga
     9061 tcaaggacaa ctgaagcgcc atcgtgacat ggggctttta tcagaagttt tcagaaatcc
     9121 agccaatttg gataaattga caggagctgg tgcgattggg catgtgcgtt atgcgactgc
     9181 tggcgaagct tctgttgata acatccagcc cttcctcttc cgttttcacg atatgcagtt
     9241 tggcttggct cataatggaa atctgaccaa tgcagcctct ctcaagaaag aattggaaga
     9301 tagaggagca attttcagtg cgacttcgga ttcggaaatc ttggctcatc tcattcgtcg
     9361 cagtcataat cctagcctga tgggcaaaat caaggaagcg ctcagccttg tcaaaggtgg
     9421 ttttgcctat atcttgctgt ttgaggacaa gttgattgcg gctcttgacc ccaatggttt
     9481 ccgtccgctt tctatcggga aaatggctaa tggagcagtt gttgtatctt ctgaaacctg
     9541 tgcttttgag gttattggtg ccgagtggat tcgtgatttg aagccaggtg agattgtgat
     9601 cattgatgac gagggcattc agtatgacag ctatacagat gatacccagt tggcgatttg
     9661 ttctatggag tatatctact ttgctcgccc tgattctaat atccacggtg tcaatgtcca
     9721 tacggcacgt aagagaatgg gagcgcaatt ggcgcgagaa tttaagcatg aggcagatat
     9781 tgtagttggt gtgcccaatt cttccctaag cgcggctatg ggatttgcgg aagaatcagg
     9841 cttaccaaat gaaatgggtc tgatcaaaaa ccaatacacc cagcgaactt ttatccaacc
     9901 gactcaagaa ttgcgggagc aaggagtgcg gatgaaactg tctgctgttt cgggtgttgt
     9961 caaaggcaaa cgtgtggtca tggtggatga ttccattgta cgtggaacaa cctctcgtcg
    10021 tatcgttcag ctcttgaaag aagcgggtgc gactgaggtt cacgttgcca ttggaagtcc
    10081 tgcactagcg tatccatgtt tctacgggat tgatatccag acccgtcagg agctgattgc
    10141 agccaatcat acggtcgaag aaactcgcca aatcattggt gcggacagta tgacttatct
    10201 ttcaattgat ggcttgattg agtcgattgg tatcgaaaca gatgcgccga acggtggtct
    10261 ctgtgtcgct tactttgacg gtgactaccc aacgcctctc tatgactacg aagaagacta
    10321 tcgtagaagt ttggaagaaa agaccagttt ttacaagtag gcgacagatt ctccattaaa
    10381 gaaaaggaaa aaacaaatga caaataaaaa tgcatatgcc cagtcgggtg tggatgttga
    10441 agcgggttat gaagttgttg aacggattaa aaagcacgtg gcccgtacgg agcgtgcagg
    10501 tgtcatggga gctcttggtg gctttggtgg tatgtttgac ctttccaaga ctggggttaa
    10561 agaacccgtc ttgatttcag ggactgacgg tgtcggaacc aagctcatgt tggctatcaa
    10621 gtacgacaag cacgatacca tcgggcagga ctgtgtggcc atgtgtgtca acgacatcat
    10681 tgctgcaggt gcggaacccc tctattttct cgactacgta gcgacaggga agaatgaacc
    10741 agctaagcta gaacaagtgg ttgctggtgt ggcagaaggt tgtgtgcagg ctggtgctgc
    10801 cctcatcggt ggggaaacgg ctgaaatgcc gggcatgtac ggcgaagacg actatgactt
    10861 ggctggtttt gcggtcggtg tggctgaaaa atctcaaatc attgacggtt caaaggtggt
    10921 agagggagat gttcttctcg gacttgtttc aagtgggatt cactcaaatg gttactcttt
    10981 ggttcgtcgt gtctttgcgg attacacagg tgaggaagtc ctaccagaat tggaaggcaa
    11041 gaaacttaag gaagttctac ttgagccgac tcgtatctat gtcaaggctg tcttgccgct
    11101 catcaaagaa gagttggtca acggcattgc ccacatcaca ggtggtggct ttatcgaaaa
    11161 tgtccctcgt atgtttgcag atgacctagc tgctgaaatt gatgaaagca aagttccagt
    11221 gcttccaatt ttcaaagccc ttgaaaaata cggtcagatt aaacacgaag aaatgtttga
    11281 aatcttcaat atgggtgtgg gactcatgtt ggcggtcagc cctgaaaatg tagagcgtgt
    11341 aaaagaattg ttggatgaag cagtctatga aattggtcgc atcgtcaaga aagaaaacga
    11401 aagtgtcatt atcaaatgaa aaaaatagcg gtttttgcct ctggtaatgg ctcaaatttt
    11461 caggtgattg ccgaagaatt tccagtggag tttgtctttt cagaccatcg tgatgcctat
    11521 gtgcttgagc gtgcaaagca gctcggcgtt ctgtcctatg cttttgaact caaggagttt
    11581 gagagcaagg cagactacga agcagccctt gtcgaactct tggaagaaca ccagattgac
    11641 ttggtttgcc tagcaggcta catgaaaata gttggaccaa ccttattgtc ggcttatgaa
    11701 ggtcggattg tcaacattca tccagcctac ttgccagaat ttccaggagc tcatgggatt
    11761 gaggatgctt ggaatgctgg cgtgggtcag tctggtgtga ccattcactg ggtggattcg
    11821 ggtgtggata caggccaggt catcaaacag gttcgtgtgc cacgactagc tgatgatacc
    11881 attgacagat ttgaagctcg catccatgaa gcagagtaca ggttgtatcc ggaagtagtg
    11941 aaggctctat ttacagattg actttttgat gattcatatg atatctttga ttttaaattg
    12001 gagtcagtgt ttgttgaaga cggcttcaaa cggaggtatt tgtaatgtta gaatctaaaa
    12061 aaacaactcg atatgtattt tatgtctatc tgatgttatt aacttgggga atcttattta
    12121 agtttgaaac aaatcctgaa tttatagcat ttttcttagc tccaaggtat atcaattgga
    12181 ttccattttc agaaccacta atagtcgatg gaaaaattgt ttttgctgaa atgttattta
    12241 atctgatttc ctttattcca ttaggtgttt gtttcccttt gataaaaact aatttatcta
    12301 gtttaagaat agtcgggaca ggtttcttga ttagtttatt gtttgagtgc ttacagtata
    12361 ttttagcaat aggtataaca gatataacgg atttgacttt aaatacgcta ggtgtctgtg
    12421 taggcttact gatttatcaa atttttataa gagtgttcaa atcacagact agaaaatgga
    12481 tcaatatctt aggtatgctt agccttggtt ttgcttatct tgttttactg ttactgcatt
    12541 taattggtgt ttaactaatg attaaaaagg agaatataat gactaaacgc gtcttaatca
    12601 gcgtctcaga caaagcgggc attgttgaat ttgcccaaga actcaaaaaa cttggttggg
    12661 agattatctc aacaggtgga actaaggttg cccttgataa tgctggggtg gataccattg
    12721 ctatcgatga tatgactggt ttcccagaaa tgatggacgg tcgtgtgaag acactccacc
    12781 caaatatcca cggagggctt ctcgctcgtc gtgacttgga tagccacttg gaagcggcta
    12841 aggacaacaa gattgagctc attgaccttg tggtggtcaa cctttaccca tttaaggaaa
    12901 ctatccttaa accagatgtg acttatgctg atgcagttga aaatatcgat attggtgggc
    12961 catctatgct tcgttcagca gcgaaaaatc atgccagtgt tacagttgtg gtagatcctg
    13021 ctgactacgc tgtggttttg gacgagttgt cagccaatgg tgaaacgact tatgaaactc
    13081 gccaacgttt agcagccaaa gtatttcgtc acacagcggc ttatgacgcc ttgattgcag
    13141 aatacttcac agctcaagtg ggtgaaagca agcctgaaaa gttgaccttg acttatgacc
    13201 tcaagcaagc catgcgttac ggtgagaacc ctcaacaaga cgcggatttc taccaaaaag
    13261 ccttgccaac agactactcc attgcttcag ccaaacagct caatggtaaa gaattgtcct
    13321 tcaacaatat ccgtgatgcg gatgctgcca ttcgtattat tcgtgatttc aaagaccgtc
    13381 caactgttgt ggctctcaaa cacatgaacc catgtggaat cggtcaggct gataacattg
    13441 agactgcttg ggactacgct tatgagtctg acccagtgtc tatctttggt gggattgtcg
    13501 tcctcaaccg tgaggtggat gctgcgacag ctgagaagat gcacggcgtt ttcctcgaaa
    13561 tcatcattgc accaagctat acggatgaag cgctagctat tttgatcaat aaaaagaaaa
    13621 acttgcgtat ccttgccttg ccatttaatg ctcaagaggc tagcgaagtg gaagcagaat
    13681 acacaggtgt agtcggtgga cttctcgtgc aaaatcaaga cgtggtcaag gaaagcccag
    13741 ctgactggca agtggtgacc aaacgtcagc caactgagac agaagcgact gctcttgagt
    13801 tcgcttggaa ggctatcaag tacgtcaaat caaatggtat tatcgtgacc aacgaccaca
    13861 tgacacttgg tgttggtcca ggtcaaacca accgtgtggc ttctgttcgc cttgccattg
    13921 accaagccaa agatcgtctg aacggggcgg tccttgcttc agattccttc ttcccatttg
    13981 cggataacgt ggaagaaatc gccaaagcag gaattaaggc catcatccag cccggtggct
    14041 ctgtccgtga ccaagaatcc atcgaagcag cggataaata cggcttgact atggtcttta
    14101 caggtgtgag acattttaga cattaagaag ataaaaggga agaaaacagt ttctttcctt
    14161 ttttggctta aaatgctaac tgaaacaaga ttaaaacgaa cttttttgat ataatattgg
    14221 taaataattc gcaaaagagg ttgaggaatg aaactgcttg ttgtcggttc gggtggtcgt
    14281 gagcatgcga ttgctaaaaa gttacttgaa tcaaaagacg ttgaaaaagt ctttgtagct
    14341 cctgggaatg atgggatgac tctggatggt ttggaattgg taaatatctc tatttccgaa
    14401 cattctaaat tgattgactt cgcaaagacc aatgatgttg cttggacctt tatcggtcca
    14461 gatgatgccc ttgctgctgg tatcgtggat gattttaacc aagctgggct taaggccttt
    14521 ggtccgacta gggctgcagc ggagctggag tggtccaagg attttgccaa ggaaatcatg
    14581 gtcaaatatg gtgtttcgac agcagcctat ggcacatttt cagatttcga ggaagcaaag
    14641 gcctatatcg aaaagcatgg tgcgcctatc gtagtcaagg cggatggctt ggcgcttggg
    14701 aagggtgtcg tcgtttctga gacggttgag caagcggtcg aagccgctca tgagatgctt
    14761 ttggacaata aatttggtga ctcaggtgcg cgcgtggtta ttgaggaatt ccttgaagga
    14821 gaggaatttt cactctttgc ctttgttaat ggtgataagt tctacatcat gccaacggct
    14881 caggaccaca aacgtgccta cgatggcgac aaagggccta acacgggtgg tatgggtgcc
    14941 tatgcgccag ttccacactt accacagagt gtagttgata cagcggttga caccattgtc
    15001 aagccagttc tagaagggat gattaaagaa ggtcgccctt atctgggagt tctttacgca
    15061 gggcttatcc tgacagctga tggaccgaaa gtcattgagt tcaacgctcg gttcggagat
    15121 ccagaaactc agattatctt gcctcgcttg acctctgact ttgctcaaaa tatcacagat
    15181 atcctggata gcaaggagcc aaatatcatg tggacggaca agggtgtgac tctgggtgtg
    15241 gttgtcgcat ccaagggcta cccgctagac tatgaaaggg gcgttgagtt gccagccaag
    15301 acagaaggcg atgtcatcac ctactatgca ggggctaagt ttgcggaaaa tagcagagca
    15361 ctgctctcaa acggcggacg agtttatatg ctcgttacca cagcagatac cgtcaaagaa
    15421 gcccaagcca gcatctacca agaactatac caacaaaaaa tagaaggact cttctaccga
    15481 acagatatcg gaagcaaggc aattaagtaa agatataaga ataacgcgcc gtagtcgcca
    15541 aacacgataa tggtcgtcgt ggtgaaaaga ccagaacagt gaatgttctg gtcaggggga
    15601 aacttggaga ccgtaggctc aaagtttagg aatgaaaccg aaggtttgct tccgcctcca
    15661 tcacctaaga ccattatcaa aaagaaaaat aaaaattcac aaaatacgtt aatgatcgta
    15721 tggtttgcga gcgttagcga gctaatatag aacaatcacc gccgttgtga aagaacgatt
    15781 ggatgataat ccaatcgttc agggaaattg gaagaccttg ggtttccaat ttaggcatga
    15841 gacacctttg gtggctgctg ccgtccctca caagctaagg tgattgttga aaaagaggaa
    15901 aaaggagaag aaatgaaacc agtaatttcc atcatcatgg gctcaaaatc cgactgggca
    15961 accatgcaaa aaacagcaga agtcctagac cgcttcggtg tagcctacga aaagaaagtt
    16021 gtttccgcac accgtacacc agacctcatg ttcaaacatg cagaagaagc ccgtagtcgt
    16081 ggcatcaaga tcatcatcgc aggtgctggt ggcgcagcgc atttgccagg catggtagct
    16141 gccaaaacaa cccttccagt cattggtgtg ccagtcaagt ctcgtgctct tagtggagtg
    16201 gattcactct attctatcgt tcagatgccg ggtggggtgc ctgttgcgac catggctatc
    16261 ggtgaagctg gagcgactaa cgcagctctc tttgccctcc gtctcctctc tgtagaagat
    16321 aagtccattg cggatgcact tgccaacttt gctgaagaac aaggaaaaat cgcagaggag
    16381 tcgtcaaatg agctcatcta aaacaatcgg aattatcggt ggcggtcaac tgggtcagat
    16441 gatggccatt tctgctatct acatgggcca caaggttatc gcgctggatc ctgcggcgga
    16501 ttgcccggcc tctcgcgtgg cggaaatcat tgtggcacct tataacgatg tggacgccct
    16561 ccgtcagttg gcagaccgtt gcgatgtcct cacttatgag tttgaaaatg tcgacgctga
    16621 cggtttggat gccgttatca aggatggaca actccctcaa ggaacagatc tgctccgcat
    16681 ttcgcaaaat cgtatttttg aaaaggactt tttgtcaaac aaggctcaag tcactgtggc
    16741 accctacaag gtcgtgactt ctagcctaga cttggcagat atcgacttgt cgaaaaacta
    16801 tgtcctcaag actgcgactg gtggctacga tggtcatgga caaaaggtta ttcgttcaga
    16861 agcagacttg gaagcagcct atgcgctagc agactcagca gactgcgtct tggaagaatt
    16921 tgtcaacttt gaccttgaga tttctgtcat cgtgtcagga aatggcaagg aggtgacgtt
    16981 tttcccagtt caggaaaata tccaccgcaa caatatcctg tctaagacca tcgtaccagc
    17041 ccgcatttct gaaagtctag tagacaaggc taaagctatg gcagtgcgaa tcgcagaaca
    17101 actcaacttg tctggaactc tctgtgtgga aatgtttgcg actgatgatg acgttattgt
    17161 caatgaaatc gccccacgac cacataactc tgggcactat tctattgaag cctgtgattt
    17221 ctctcagttt gacacccata ttctcggtgt tctcggagca ccattaccag tcatcaaact
    17281 ccatgcgcca gccgttatgc ttaatgtcct cggtcagcat gtcgaggctg ctgagcaata
    17341 tgtgacagaa aattcaagcg cccacctcca catgtatggt aaaatagaag cgaagcacaa
    17401 ccgcaagatg ggacatgtga ccttgtttag tgatgtaccg gatagtgtgg atgagcttgg
    17461 ggaagggatt gatttttagg acaagtctat gatacaaatt atcgttaata catttattga
    17521 aaaggataag actggagcag ttgttgaagt gttgtatgcc agtgctgacc aagataaggt
    17581 aaaagctaaa tatgaagaac tagctgcaca ataccccgaa aattatttag ctatctatga
    17641 tgtaccgctg gatacggatt tgaatacact agatcattac ccgtctgtgt ttattgaaaa
    17701 agaggagttt gagtagaaat cttggtttac ctagatagct tattcccaac agcttaagaa
    17761 gaaaggaaaa attaacacat gatcaaccgt tactctcgcc ctgagatggc gaatatttgg
    17821 agtgaagaaa ataaataccg tgcttggctt gaggtggaaa tcctctctga cgaggcatgg
    17881 gctgagttgg gggaaatccc taaggaagat gtggctttga ttcgcaagaa ggcggacttt
    17941 gacatcgacc gtattttgga aattgagcag gagacgcgcc acgatgtggt ggctttcacg
    18001 cgtgcggttt ctgagactct tggtgaagag cgcaagtggg ttcactatgg gttaacttct
    18061 accgacgtgg tggatactgc ttatggttac ctctacaagc aggccaacga catcatccgt
    18121 cgtgaccttg aaaacttcac taatatcatc gctgacaagg ccaaggagca caagttcacc
    18181 atcatgatgg ggcgtactca tggtgtgcac gctgagccga caacctttgg tcttaaattg
    18241 gcaacttggt acagcgaaat gaaacgcaat atcgagcgtt tcgagcatgc agctgctggt
    18301 gtagaagctg gtaagatttc tggtgcggtt gggaactttg ccaatatccc accatttgta
    18361 gaggagtatg tctgcgataa acttggcatc cgtgcccaag aaatctctac acaagtcctt
    18421 cctcgtgacc ttcacgctga gtactttgcg gttcttgcca gcattgcgac ttcaatcgaa
    18481 cgtatggcga ctgagattcg tggtctacaa aaatctgagc aacgcgaagt agaagagttc
    18541 tttgctaaag ggcaaaaagg gtcttcagca atgcctcaca aacgcaaccc aatcggttct
    18601 gaaaatatga ctggtctggc gcgtgtcatt cgtggtcaca tgattacggc ttatgaaaac
    18661 gtcgctctct ggcacgaacg cgatatttct cactcatcag ctgagcgtat catcacacca
    18721 gatacgacca ttttgattga ctacatgctc aaccgttttg gaaatatcgt caagaacttg
    18781 acagtcttcc cagaaaatat gatccgaaac atgaactcga cttttggtct tatctttagc
    18841 caacgggcta tgttgacatt gattgaaaaa ggcatgaccc gtgagcaagc ctatgacttg
    18901 gtgcaaccaa aaacagccta ctcttgggac aaccaagtag actttaaacc acttcttgag
    18961 gcagattcag aagtaacatc acgtctcact caagaagaaa tcgatgaaat cttcaaccta
    19021 gtttattaca ccaaacgagt ggatgatatc tttgaacgtc ttggactagg tgattaatta
    19081 aaaaataaac agcgagcttc aatctcgctg tttatttttt atcgaaaaga cttagtcttc
    19141 ttttctttta gtgagtccat aggctgctag tgtggacatg agtcctgcga ctactagtcc
    19201 tgcagaatcg tgagttcctg tttcaggaag ttttttctct gttaccacag gagctggatc
    19261 ttgaggaaga gctttgcttt cctcagcggg agcagttgat ggagctggtt ggcttgggat
    19321 ttctagtttt ggtttttctt cagcaatagc ggcttgtccg ttttcatcgc ctacatgtgt
    19381 taccatagtt ccgacttcga ctatttgagt aacggcttcc tgtgctacga cactatttac
    19441 aagtgttttc acttccttac catcggcaga agtgctcaca gagtagaagt tgctacgatg
    19501 tccattgacg cccttagtaa tgacttgtgt ttttcctttg agtaagagtg gattttcacg
    19561 agtcactgtg gtaaatggaa tttcttcttt ttggatatcc agtctaggtt ttacctcagt
    19621 agttggtgca agaccacttt catcaccctt gtgagttaca ggagcgccaa cttcaaccac
    19681 ttggtttata acttctttgg ttacctggct atcaaggact gtttctgttg tttttccatt
    19741 ttcagtgagt acagagatgt aatgagttcg ttcacctttg actcctgctg tgataatatt
    19801 ttcctgacca gctgggaggt taggattttc tttcttgata acttcaaatg gaatttcttc
    19861 agttcttgtg atgagttctg gtctggtttc aacattggca gccacttcat tttcatccag
    19921 gcttcctgaa tgagttgcag ctggtttgag gccttgaaga gcggctttta ggttggctac
    19981 aagtgtgtca agctcagctt gtttattacg gttgaggttg taatttagag ctgttttagc
    20041 tgcgtcaagg gcctcaagac tttctttact atatccttct aagtttgtag gaattttagc
    20101 taattcttcg cggagagcat tataattagc acggaagtag tctttgttgt ggtctgcaaa
    20161 ggcagtcatg agttcaaaga tttcctcttc cttgtactca gcgcttggtc tatctgccca
    20221 gattgaaagc atacttccga ctgttggaag atctacttca ggatatttgg tagaagctag
    20281 ttgattgaat ggtgtttttc cagtattctc aatagctttc ttgaggaaac caccaccatc
    20341 ttctggtttt tgaccaagaa tgtagtacca gtcaccgttg gtattcaaga atttatagcc
    20401 tttgcttgct aggtattgag gtgatgcgag gttatatccc caccagcctt tagaccagta
    20461 agaaatcaag acatctttgt caaactgaac atcgtccttg tcttcatagt agaagccatc
    20521 gttgaaggcc attggttgaa gccctctttc tttggccata gctgcgaggg tgttggcata
    20581 ttcggcaaat ttgccataga gttgatacca cttgaggtag taccagcctt gggcactagt
    20641 cgcatcgttg gcgtattcgt cagtaccaaa gttgaaaatc tttgttttac ctgcaaagaa
    20701 gtccatgtat ttaccgatga gggcttttac aaagttcatc gcttcttcgt ttttcaagtc
    20761 catagttgtt tttgaaactt tatcaaagtg ggcttgagga tttttaatac ctaatttttc
    20821 catggcaacc agcatagcat ccatgtgacc tggactgtta atagctggga tgagaccgat
    20881 gtccttagat ttagcgtatt caattagctc tgttacttct gcctgtgtta gtgcagtacc
    20941 gtttggatcg tcgtagtaag ctttagttcc ttcgataata gcttttttaa cgtcatcact
    21001 agcataggtt tttccgttgg cagtaatggt catatcatcg agtagaaagc gaagtccgtc
    21061 atttcctaga aggagatgga catcagaata tccgagctca ctggccttgt ctacgatgcg
    21121 tttgagctgg ttcagagtaa agtatttgcg tccagcatcg attgagatta ccttgttttt
    21181 ggcaagtttt tcaacctcac gtttagcttc ttcttctttt agagcttcag gcgtgagggt
    21241 caagttgttg acagtttctt gaagtttagc aatggcttga tcaatcgtat cttgttgggc
    21301 acggctaagg ttgctatcga gagagcgaat agctttttca gcttctttta cggccgtgac
    21361 gctttctgca gtataacggt tcaggtcttt tggtacctcg ttaagtgctt gctctgcaga
    21421 ttcataatca gctgcgaagt attcagcgtt ggcatttgca aaatgacgca tgagtttgaa
    21481 gaggcgtgat ggtgaataac gtgcagatgg agtgtcagcc caagcagcta ccataccacc
    21541 gatgattggg atatcagctc cttctgtttt tggtacagaa gtgattggtg tgtttttaat
    21601 accattgagc ccctgatcga gattgtacca gccttggcca tcagcgtttc gtccaagaac
    21661 gtagtaccaa gcatcattgg tattaaggat ttggtgacct ttttcagcta gtagtttaga
    21721 agaagcgaca tcgtagcctc cccaaccacc agtccacata gaaacgatga tgtctttgtc
    21781 aaaactacca aagcttgtgt cgctattgta gtagataccg tcgttaaaag ccattggttt
    21841 gagaccgtgc gattttacaa tacgagcgag gtcattggcg taggcaataa atttttcata
    21901 gccttttaca gggtagcctt cgtttggata gtatttatca gcttgaagca cactccaacc
    21961 tttagcatct gtcgcatcat tggcatattc atcaagtccg atgttgaaga tttcagtctt
    22021 tttcgcgaaa taagcagcat acttgtcgat aagggctttt gtaaaagcga cagcttgttc
    22081 gttgtcaaga tcgacagtac gggctgattt cttcccaaaa tagctaaagt tagggttttg
    22141 gattcccaat tctttcatgg cattgagaat cgcatccatg tgtccaggac tatttactgt
    22201 cggaatgaga ccgatacctt tatctttggc atagttaatc agatctgtca tttgactttc
    22261 tgttaagtga ttgccgtttg gatcgttgta ataatcattt gtaccttttt caatggcgcg
    22321 tttgacatcg tcactggcat aggtcttgcc gttagctgtg atgctcatat cgtccaacat
    22381 gaaacggagt ccatcatttc cgactaatag gtgtaaatca gtgtagccat aatgtttcgc
    22441 tttatcgatg atttccttga gttgttctgg tgagaaatat ttacgtccag catcaataga
    22501 aacaattttc tttttcgcta gtttttcatt tacagttgca gcacgttcct ttcctgcctc
    22561 tgtttccggt ttgtcagcct ctgctttcgc ttcatctttt ttagctggtt tatccttgtc
    22621 agtcttgtct gtatttgact ctttagaatc aacctctttc gcttcttcct ttttagggct
    22681 agcttcttct gcctttttat tagcagtttc tttttcagca gaagttggag ttaccacttc
    22741 tgctttatca ctaggagttg aactaacttc ctcttgtggt ttttcttctg tttttggaag
    22801 actagctacc ttatcagtag ctggagtttc tgtttctaca gtttttggag cttctggttg
    22861 aagcactgct ttaggtgttt cctcagtccg attttcggat gattgagggg aatcagaaac
    22921 cgtatggatg gtcggttggt tttctgtagt agtaggagta actccatcgg ctgcaacagt
    22981 ctgtgcttgg aaggcaaatc caattagaac agaagctgct cctacagcgt atttacgaat
    23041 agaaaaacgc tgttgttttt catgtttcat tgcaaaacct cctgattgca ttgttatatt
    23101 gatagcgatt atataaatca acgcctttat tttatttctt atattaacga gagtcaagag
    23161 gagatgacaa aaaactataa taagtataaa aaaatataaa atttaaactt aagatttcag
    23221 attggtcgga aaaaatacgt atatatatct agtataattt ttggttctat ttctataaaa
    23281 tattccacaa attatagaat tttccaaaaa taggtaagcg ctaccttttt ggtgtggtat
    23341 aataagcata gaaaaagccc aagcgattag ctcaggcttt cttcttagtg atcacggtca
    23401 catgagataa atttaatctt gtagtaatca gatcgtttgt aagtttcact gtattctaaa
    23461 acttggccag ttgattcgag tttggtgatt ttagtttgta ggacagtagg gaattgttca
    23521 tcgactccga ggactgaagc cgcatgttct ggagttggaa agactatttc gttgatttct
    23581 tcaaagtgtt catcattcat gtgaatgtgg tagtctaact tgaaacgatt atagatagaa
    23641 ctatagtatt caaggtttgg ataatttgcg ttgatatatt gttctgggat gtaggatgta
    23701 tggtagatat aaacgacacc gtttgattcg cggatacgtt caatcttgta gtagaattga
    23761 tcgccacgta gacccaattt ttccaagtaa acaagcttgt ttccgcgttc aattgaaaga
    23821 acagttacct tatcatcttt agcattgaag agttcaatat ctgaaaactc tacaagcttg
    23881 tgtttgcgtg cacgtgaaac gaaggttcct tttccttgtt ggcggacaat atagccatct
    23941 ttggcaaggt cgtttaaggc gcgaacaact gtgatagagc tgacatcgta cattgaaatg
    24001 agttctgctt cagtgtaaaa tttatctcca ctgctaaact gcccagagat gattttattt
    24061 tttaattcgt cttttatgta ttgatatttg ggaattgcca tatttttacc tctattttcc
    24121 ttctccatag aaaattttac cataaaagcg aaaaaaatag tagtcttttg aataaaaaat
    24181 tagaataata gtctgaaa
//
LOCUS       DAVKJJ010000033        23028 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000033 DAVKJJ010000000
VERSION     DAVKJJ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23028
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.033"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            121..3222
                     /locus_tag="VW144_001329"
     CDS             121..3222
                     /locus_tag="VW144_001329"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU9600774.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSIEAGHTAEKAEQVFDVLEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQIMLNSANSDYVTDALEAGFEVAPLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIEHRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            3305..4312
                     /gene="pfkA"
                     /locus_tag="VW144_001330"
     CDS             3305..4312
                     /gene="pfkA"
                     /locus_tag="VW144_001330"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU9600775.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEADSKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4371..5876
                     /gene="pyk"
                     /locus_tag="VW144_001331"
     CDS             4371..5876
                     /gene="pyk"
                     /locus_tag="VW144_001331"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU9600776.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDETREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6567..6792)
                     /locus_tag="VW144_001332"
                     /pseudo
     CDS             complement(6567..6792)
                     /locus_tag="VW144_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(6950..7204)
                     /locus_tag="VW144_001333"
     CDS             complement(6950..7204)
                     /locus_tag="VW144_001333"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9600777.1"
                     /translation="MGTKIGISKNTIGNYEKRVRSTKKNTIFDLVKVFSSLIDALFSP
                     VQKDSPSDIQSIYDQRAPSRQGKVLTYAERQLYDQKNEVS"
     gene            7640..8512
                     /locus_tag="VW144_001334"
     CDS             7640..8512
                     /locus_tag="VW144_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEU9600778.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPTDDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8645..9002)
                     /locus_tag="VW144_001335"
                     /pseudo
     CDS             complement(8645..9002)
                     /locus_tag="VW144_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            9145..9456
                     /locus_tag="VW144_001336"
     CDS             9145..9456
                     /locus_tag="VW144_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600779.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEHPELYAVAQTSGEIPEKECEELRQIITTIFEQKMNKHSI
                     K"
     gene            9460..10176
                     /locus_tag="VW144_001337"
     CDS             9460..10176
                     /locus_tag="VW144_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9600780.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEEFICLIGHSLGGDLARYLASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVLDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(10305..11120)
                     /locus_tag="VW144_001338"
     CDS             complement(10305..11120)
                     /locus_tag="VW144_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEU9600781.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQVTNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(11117..12022)
                     /locus_tag="VW144_001339"
     CDS             complement(11117..12022)
                     /locus_tag="VW144_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEU9600782.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSIVVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSHLSPAKKVFQVFVQAIDEFFDTGRYLVFGCLFASVIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
     gene            12434..14563
                     /locus_tag="VW144_001340"
     CDS             12434..14563
                     /locus_tag="VW144_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEU9600783.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGKKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            14550..14999
                     /locus_tag="VW144_001341"
     CDS             14550..14999
                     /locus_tag="VW144_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEU9600784.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPLKLYRCQSCQQRYQRKRRIDTKRYRCGLCRGKLLLINQPED"
     gene            15058..15330
                     /locus_tag="VW144_001342"
     CDS             15058..15330
                     /locus_tag="VW144_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9600785.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGRFW"
     gene            complement(15684..15875)
                     /locus_tag="VW144_001343"
     CDS             complement(15684..15875)
                     /locus_tag="VW144_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600786.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            16303..17061
                     /locus_tag="VW144_001344"
     CDS             16303..17061
                     /locus_tag="VW144_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9600787.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            17063..19051
                     /locus_tag="VW144_001345"
     CDS             17063..19051
                     /locus_tag="VW144_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9600788.1"
                     /translation="MFRLTNKLAVLNLIKNRKLYYPFALAVLLAVTLTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVVITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDNGYKIKEKEVFRYTYFGVANQEGNKLTFFEKGQNRVQPTTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGL
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(19093..19737)
                     /locus_tag="VW144_001346"
     CDS             complement(19093..19737)
                     /locus_tag="VW144_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEU9600789.1"
                     /translation="MLRAGVLGANDGIISIAGVVIGVASATTNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNGECKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSEYR
                     IPATVLIVGVALLLTGYTSARLGKDPTRTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(19709..20701)
                     /locus_tag="VW144_001347"
     CDS             complement(19709..20701)
                     /locus_tag="VW144_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9600790.1"
                     /translation="MLQKANKGKSNREIASLLGKVPQTIHTEIKRGTVRQCLGKGRFK
                     EVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQKFSPEMMVMAKGVNVGIST
                     IYYWIHHGKLGLSKQDLPYPRKGKALKKQASTNFKPAGQSIEQRSEAINLRLENGHYE
                     IDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSAEVVNQALKLILKQHKILSITADN
                     GTEFNRLFDVFSEEHIYYAHPYASWERGTNENHNRLIRRWLPKGTKKMTPKEVAFIEK
                     WINNYPKKCLDYKSPREDFWMANLNLKFSVRNKSRNRFKFCRSFKYPARRCSWC"
     gene            21421..22896
                     /locus_tag="VW144_001348"
     CDS             21421..22896
                     /locus_tag="VW144_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU9600791.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINTIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
BASE COUNT         7000 a         4219 c         4883 g         6926 t
ORIGIN      
        1 atgacaaaaa tacaaatgga tagaaaataa attagtaagc tgatgaaatt tttctcaaga
       61 gaagccattt ataggtgaaa atggtataat atagtgagaa ggatagagga gaagtgtaaa
      121 ttgatcgcac aactagatac aaaaacagtc tatagtttta tggaaagcgt catttcgatc
      181 gaaaagtatg tgagagcagc taaagaatac ggctacactc atttggctat gatggatatt
      241 gacaatcttt atggcgcttt cgactttcta gagattacaa aaaaatacgg cattcatcct
      301 ttgctagggc ttgaaatgac agtgtttgta gatgatcagg aagtgaattt gcgcttttta
      361 gctctatcta gtgtgggcta tcagcagttg atgaagcttt cgacagccaa gatgcagggg
      421 gagaaaactt ggtcagtcct gtcccagtac ctggaggata tcgcggtcat tgtgccttat
      481 tttgatagag ttgagtcgtt agaactaggc tgtgattact atataggggt ttatccagaa
      541 acactagcaa gcgaatttca tcatcctatc ttacctcttt atcgggtcaa cgcttttgaa
      601 agcagggata gagaagttct tcaagtttta acagcgatta aagaaaatct accgctcaga
      661 gaagttccct tgcgttcgag acaagatgtc tttatatcag caagttcttt agagaaacta
      721 ttccaagagc gttttccgca agctttggac aatttagaaa agcttatttc aggcatttct
      781 tacgacttgg atactagtct gaaactgcct cgttttaatc cagctagacc agcagtagag
      841 gagttgagag agcgtgctga actggggctt gttcagaagg ggttgactag taaagaatat
      901 caagatagac tagaccaaga attgtctgtt attcatgata tgggctttga tgattatttc
      961 ttggttgttt gggatttgtt gcgttttgga cgatcgaatg gctattatat gggaatggga
     1021 aggggttctg cagtaggcag tttggtttct tatgccttag acatcacggg gattgaccca
     1081 gtagagaaaa atctgatttt tgaacgcttt cttaatcgtg aacgctatac catgcctgat
     1141 attgatattg atatcccaga tatttatcgt ccagatttta tcagatatgt tggtaataaa
     1201 tatggtagta aacatgcggc acaaatcgtt actttttcaa cctttggagc caagcaagct
     1261 cttcgagatg tcttgaaacg ctttggtgtg ccagagtatg aattatctgc aattactaag
     1321 aaaatcagtt ttcgtgacaa tcttaagtcg gcctatgagg gcaatctcca gtttcgtcag
     1381 caaatcaata gtaagttaga ataccaaaaa gcttttgaga ttgcttgcaa gatagagggc
     1441 tatccaaggc aaacctctgt ccatgcggct ggtgttgtaa ttagtgacca agatttaacc
     1501 aactacattc ctctaaagta tggtgatgaa attccactga ctcagtatga tgctcatgga
     1561 gttgaggcta gcggactttt gaagatggac tttctgggac tacgaaattt gacctttgtc
     1621 cagaagatgc aagagttgct tgctgaaaca gaaggtattc atctgaaaat tgaagaaatc
     1681 gatttagaag acaaagaaac gttagcttta tttgcctctg gtaatacaaa aggtatcttt
     1741 caatttgagc aaccaggtgc cattcgtctg cttaagcgtg tgcaaccagt ctgttttgaa
     1801 gatgtcgtcg cgactacttc tctaaatcga ccgggtgcta gtgactatat caataatttt
     1861 gtggcaagaa agcatgggca ggaagaagtg actgttctgg atccagtact ggaggatatt
     1921 ttggctccaa cctacggcat aatgctctat caggagcagg ttatgcaggt tgcccagcga
     1981 tttgctggat ttagtcttgg gaaggccgat attttgcgtc gagctatggg taaaaaggat
     2041 gcttctgcta tgcatgagat gagggcttcc tttattcaag gctccataga agcaggccat
     2101 actgcggaaa aagcagagca ggtttttgat gttctggaga agtttgcagg ctatggtttt
     2161 aacaggtcac acgcctacgc ctactcagcc ttggccttcc agttggctta ttttaaaaca
     2221 cattatccag ccatatttta tcagattatg ttgaattctg ctaacagtga ttatgtaaca
     2281 gatgccttag aagcaggttt tgaagtagcc ccattgtcca tcaacacgat tccctatcac
     2341 gataaaattg ccaacaaggc catctatcta ggtttgaaat ccattaaagg agtcagtaat
     2401 gatttagctc tctggattat tgaacataga ccttattcta acattgaaga ttttatagct
     2461 aaattacctg agaattatct gaaacttcct ctgctagaac ctttggtaaa agttggtctt
     2521 ttcgattcat ttgaaaaaaa tcgtcaaaaa gtatttaata acttagctaa tctatttgaa
     2581 tttgtgaaag agttgggaag tttgtttgga gatgctattt atagttggca ggaatcggaa
     2641 gattggacgg aacaagaaaa attttatatg gaacaagagc ttttagggat aggtgtcagc
     2701 aaacatccac tacaagctat tgcaagtaag gctatttacc cgattacccc aatcggaaat
     2761 ttgtcagaaa atagctatgc tattattttg gttgaagttc agaaaataaa agtgattcgt
     2821 accaaaaagg gtgaaaatat ggccttctta caggcagatg atagtaagaa aaaattggat
     2881 gtcactctct tttcagactt atatcgtcag gttggacagg aaataaaaga gggagccttc
     2941 tactatgtaa aaggaaaaat acaatcacgt gatggccgtc tgcaaatgat tgcacaagaa
     3001 ataagagaag cagttgctga acgcttttgg atacaggtga aaaatcatga atcggatcaa
     3061 gaaatttcac gtattttaga acaatttaaa ggcccaatcc cagtcatcat ccggtatgaa
     3121 gaggaacaga aaaccatcgt ttctccccat cattttgtag ctaaatccaa tgaattagag
     3181 gagaaattga atgaaatcgt tatgaaaacg atttatcgct aaaaatacgg aaaatagaag
     3241 aattttaaaa tcaaatgtga tataatcaag aagaatgtta aaagaaaaag gagcataaac
     3301 caatatgaaa cgtattgctg ttttgactag tggcggagac gcacctggta tgaacgctgc
     3361 tatccgtgca gttgttcgtc aagcaatttc agaaggaatg gaagtgtttg gtatctatga
     3421 cggatatgct ggtatggttg ccggtgaaat tcatccccta gatgcagctt cagtaggaga
     3481 catcatttct cgtggtggta cttttcttca ctcagctcgt tatccagagt tcgctcaact
     3541 tgaagggcaa cttaaaggga ttgagcaatt gaaaaaacac ggaattgaag gtgtagttgt
     3601 tatcggtggt gacggatctt accacggcgc tatgcgtttg actgaacatg gcttcccagc
     3661 tattggtctt ccaggtacaa tcgataacga tatcgttggt actgacttta caatcggttt
     3721 tgacacagcg gttactactg ccatggacgc tattgataag attcgtgata catcatcaag
     3781 tcaccgtcgt acttttgtaa tcgaagttat gggacgtaac gctggtgata tcgctctttg
     3841 ggctggtatt gcaactggtg ctgatgaaat catcatccct gaagcagact ccaagatgga
     3901 agatatcgta gcaagcatca aagctggtta tgaatgtggt aaaaaacaca atatcatcgt
     3961 cttagctgaa ggtgtgatgt cagcggctga atttggtcaa aaacttaaag aagctggaga
     4021 tacaagcgac cttcgtgtaa cagaacttgg acatattcaa cgtggtggtt ctccaactgc
     4081 gcgtgaccgt gttttggcgt cacgtatggg tgcacatgct gttaaacttc ttaaagaagg
     4141 tatcggtggt gttgcggttg gtattcgtaa cgaaaaaatg gttgaaaatc caattcttgg
     4201 tactgcagaa gaaggggcat tgtttagcct tactgcagaa ggtaagattg tggttaacaa
     4261 cccacacaaa gccgatattg agctatctag cttgaataag agcttgtcat aatttacaat
     4321 ttagttaata agaccaaaaa ggtcatatat ataaaggagt cacaaaaatc atgaacaaac
     4381 gtgtaaaaat cgttgcaact ttgggtcctg cggtagaaat ccgtggtggt aaaaaattcg
     4441 gtgaggacgg atactggggt gaaaaacttg atgttgaagc ttcagctaaa aacattgcta
     4501 aattgattga agctggtgct aacacattcc gattcaactt ctcacacggc gaccaccaag
     4561 aacaaggtga gcgtatggca actgttaaac ttgcggaaaa aattgcaggt aaaaaagttg
     4621 gtttccttct tgatacaaaa ggacctgaaa tccgtacaga attgttcgaa ggtgaagcta
     4681 aagaatattc atacaaaact ggtgaaaaaa ttcgtgttgc aactaaacaa ggaatcaaat
     4741 caactcgtga agtgattgcg ttgaacgttg ctggtgctct tgatatctat gatgatgttg
     4801 aagttggtcg tcaagttttg gttgacgatg gtaaacttgg tcttcgtgtg gttgctaaag
     4861 atgatgaaac tcgtgaattt gaagttgaag ttgaaaacga tggtatcatc gctaaacaaa
     4921 aaggtgtgaa catccctaac actaaaattc ctttcccagc tcttgctgaa cgcgataacg
     4981 acgatatccg tttcggtctt gaacaaggta tcaacttcat cgcaatttca ttcgtacgta
     5041 ctgcaaaaga tgtgaacgaa gttcgtgcaa tctgtgaaga aactggaaac ggacatgttc
     5101 aattgttcgc taaaatcgaa aaccaacaag gtatcgataa cttagacgaa atcatcgaag
     5161 cagctgatgg tattatgatt gctcgtggtg atatgggtat cgaagtaccg ttcgaaatgg
     5221 ttccagttta tcaaaaaatg attatcaaga aagtcaatgc tgcaggtaaa gttgttatca
     5281 ctgcaacaaa catgcttgaa acaatgactg aaaaaccacg tgcaactcgt tcagaagtat
     5341 cagacgtatt caacgctgtt atcgacggaa ctgacgctac aatgctgtca ggcgagtctg
     5401 caaacggtaa atacccactc gagtcagtaa ctacaatggc tacaatcgac aagaacgctc
     5461 aagctcttct taatgaatac ggacgtcttg attcagattc atttgagcgt aactctaaga
     5521 cagaagtaat ggcttctgct gttaaagatg ctactagctc aatggatatc aaattggttg
     5581 taactcttac taagacaggt catactgcac gtttgatttc taaataccgt ccaaatgctg
     5641 acatcttagc attgacattt gacgaattga cagaacgtgg cttgatgttg aactggggtg
     5701 ttatcccaat gttgacagat gctccatctt caactgacga tatgttcgaa atcgctgaac
     5761 gtaaagcggt agaagcaggt ctcgttgagt caggcgatga tatcgttatc gttgctggtg
     5821 tgccagtagg agaagctgtt cgcacaaaca caatgcgtat ccgcacagta cgttaagaaa
     5881 aatataaaaa cctatcatat ccagctttct agtctgtatg atgggctctt tttttgtcca
     5941 ggggggcaaa aagggggcaa aactttcata atggattgat actcttcgaa aatcaaattc
     6001 aaaccacgtc agcgtcgcct taccgtactc aagtacagct tgcggctagc ttcctagttt
     6061 gctctttgat tttcattgag tatgaaataa gatatgcaca aattgattag gaaattcaaa
     6121 tgaatttcta caaatgtttt agcaatcgta atgtacttgt ctagattcga tctgatatat
     6181 tttcgattta atgatatggt atttaaaacc tccaaagtag cttgctccat tcttttactt
     6241 acgtgagtgt agatgttatt tactgtttta gcgtttttgt gttccactct aaccattata
     6301 gcattcttct cagctagtgt actaaggagt gtgtgcctga aaatatggga actaaggggc
     6361 tggtttatcg gtttctctag tttagtattt gccttttgca aagtgatgtt aaatgccttt
     6421 ctctaaattt acatatcact attgtttaac aaaatctaat ctactttagg tcacttattc
     6481 tttttttgaa atgtagaatg aactttttca aagtttttcg aatcttttaa aatctgtttg
     6541 ttttatatcg ccattctccc ccttttttaa ttctccctat atagcctgac aactttcccg
     6601 atggtacgaa tatggttgct ttcgtctagg tggatgtcgg ggtatttggg attgagtttt
     6661 tttaaggtag ccttggcgga gtttcttgac atagttagcg ccgtctactt ggaagatgcc
     6721 gatggtatta tagtcaatct gtggggtatt cttgataaat aggtagtcgc tgtttcttat
     6781 ctttggctcc atggacttgc tgatgacata agcgattggg tcgtagtcgt ctgggataat
     6841 ggaaactcca tatctaaatc gttgtcctgc atcgagcggc tacctgcaga gataaactat
     6901 ctaacacgag agtaagtagt ctgtctgtag tcgtccagtc tgatgatttt tacgatactt
     6961 cgtttttctg atcatacagt tgcctctcgg cataggtcag aactttacct tgtctggatg
     7021 gtgcccgttg gtcgtagata gattggatat cgctaggaga atccttttga actggagaaa
     7081 agagggcatc aatcaagcta ctgaatactt taactaagtc aaatatagta tttttcttag
     7141 tagacctaac ccttttttca taatttccaa tggtgttttt acttatacca atcttagtac
     7201 ccaattctta ttgagttcaa ccattactag tctatattgt tttatagttg attgagtttg
     7261 gaatagtacg ctgtagctgc taaaacattt ctaggaatta atttgccttt cctaatagag
     7321 ttgttcatat cttatttcaa tctactatat ttttcgcctc taacaatcgc aatctcttct
     7381 ttatctacga acgaaatcgc tttctatttt tgtaagtaaa gcatcacacg aaatccacga
     7441 aaatgaaaac ctttgttgtg ttttcgtaaa aaatttgttg acagagcacg aaacgcctac
     7501 tataattaaa tcaagcttaa ggaaataaca aaaaaacaaa tcggagggaa agacgatgaa
     7561 cacattaaat gagaaagtaa tcaatatctg taaagcaata gttaaagaaa ctttaatcca
     7621 agacatttag gagaaagaaa tgaagaaatc aagaaaatta gccacattag gaatctgttc
     7681 agctttgttt ttaggcttgg ctgcttgtca acaacaacat gctacttctg aggggacgaa
     7741 tcaaaggcaa agcagttcag cgaaagttcc atggaaagct tcatacacca acctaaacaa
     7801 ccaggtaagt acagaagagg tcaaatctct cttatcagct cacttggatc caaatagtgt
     7861 tgatgcattt tttaatctcg ttaatgacta taatactatt gtcggctcaa ctggcttatc
     7921 aggagatttc acttccttta ctcacaccga atacgatgtt gagaaaatca gtcatctctg
     7981 gaatcaaaag aagggcgatt tcgttgggac caactgccgt atcaatagtt attgtctttt
     8041 gaaaaattca gtcaccattc caaagcttga aaagaatgac cagttgcttt tcctagataa
     8101 tgatgcgatt gataaaggaa aggtctttga ttcacaagat aaggaagagt ttgatattct
     8161 attttcgaga gttccaactg aggcaactac agatgtcaag gttcacgctg aaaagatgga
     8221 aacattcttc tcacaatttc aattcaatga aaaagctcga atgctgtctg tagtcttgca
     8281 cgacaatctg gatggcgagt atctgtttgt aggccacgtt ggggtcttag tacctactga
     8341 tgacggtttc ttatttgtag agaaattgac tttcgaagag ccctaccaag cgattaaatt
     8401 tgctagtaag gaagattgct acaagtattt aggcaccaag tatgcggatt atacaggcga
     8461 gggactggct aagcctttta tcatggataa tgataagtgg gttaaacttt aatagtgtta
     8521 tgatatgaca gtaatcttag gatttttatt tctatttttg atgagttggt gggttatttc
     8581 aatcgttaat agccagaaac cacgcaaaaa tgaaacattt attggctata tccaaagata
     8641 cgatattgat gggaatggct atcaatcatt accgtgtcct cagaactgag aggagttctt
     8701 gaaatcgtaa aactactttg aacaagagtt acttcaaccc attggatccg acggagtaag
     8761 ttgctttcgt ggataccaaa aacagccgca atttcttcat aagtgcggta ttctcgcaca
     8821 tattgaagag tggccataag aaggtcttct aggcttaatt taggttttcg tccccctttt
     8881 gtgtgtttaa gttgataagc agtttttaat acagctaata tctcttcaaa agtggtgcgc
     8941 tgaacaccaa caagacattt aaatcgtgca tcagttagtt gtttacttgc ttcataattc
     9001 atagaactac tataccatat tttgtttcgc aggaagtcta atgctaaaat cttatcagtg
     9061 agaagatttc ttaaagttta ttctttgtaa tttcatctgt attagtacat aatttaactt
     9121 agagaaaata ggaggactaa tcgaatggaa ttaaaagatt ttacagaaaa agaacaggaa
     9181 atgattaaga aaaggcttac aatgtctaat attagtgaca aagaaactac tgagaagatt
     9241 ctggcgctag taccacaaga cttgattaag cgaatcccgt tttttgtcag aaaacatgct
     9301 acaacacgta cgattaaacg catttcaatt gaacaccctg aactctacgc tgtagctcaa
     9361 acaagtggtg agattccaga aaaagaatgc gaagaattgc gtcagattat cacaactatc
     9421 tttgaacaaa agatgaataa gcatagtatt aagtagagaa tgaaaaaata ttttattggc
     9481 ggtttgggaa gcaatgccta tcatagcaag gattttcttc aagaactaga ttcgcaggtc
     9541 tattttctaa atccatatga aaagcatctt cgagatgaaa cagaattgaa atcatggttt
     9601 aaaaatgaga ttgtagagga agaatttatc tgtctgatag gtcattctct tggaggagat
     9661 ttagctcgtt atctcgcatc ggaatttgaa gaagtaaaga aactgattct tttggatggt
     9721 ggctatctag atttagataa gattttacct ttggatacag agttagagga aactaaaaat
     9781 tatatcaaat ctcaaattgt tttggactta gatgttctta cttctaaaga aaaatctgaa
     9841 gcaaagcatt ggtcagaaaa tatggagaaa gctgtaagac agtcctatca ctggaatgtt
     9901 gagtataata gatatgagtt ggctataaat tatgaaaata tagaagcgat actccgccta
     9961 cggagaaaaa tacaagcttt taagagagaa gtgggagata ccttgtttat tagtcctcgc
    10021 tatcctaatg aagctacatg gagagaggaa gccctaaaag aattgccaga ctattttgat
    10081 actatttttt tagaaaactt tggccatgag ctttatactc aagcacctaa agaaatcgct
    10141 agtctaatga atgagtggct cgcttatttt ctatgagcta cttgtattgc tggaaatggt
    10201 ataagatgaa aatctacaag tcactaattt tcatataaca aaaaacgaac aggctctttt
    10261 tctgttattc agaagagaac ttgttcgttt ttatcttgat tttcttaaaa agctctatat
    10321 acatagggat tttctggttt atcgactttg gtaaagctgt cgatttccaa ggttggaagg
    10381 ttttgtttga gttctttatg gtagtgattg accagttttc ctttggctgt tatccaagtg
    10441 ttgttttcat actgccgggt attgcccttg gtcagcaatc catagacacc agaatctgcg
    10501 atacagtgga taatgccgaa tcggaacaga aattgactat tggcatgact ggggtcgtta
    10561 tagacaaagc ctgtaaactg gattgtcttg ccctcaaact catctggata gtcgtagata
    10621 gcctccatga cttccatata gttttcatta gtgacctgaa tactatcttg ggataagtat
    10681 ttatccgccg ccgttcgcat ttccttttca taggctgatt ttgaaaaata agaactggta
    10741 tctggtttca aatattggct tgtcgtccct tcgcttgtct gaatggctag atccgttcct
    10801 tccgataggg ggaaatgata acctttagca gaaacagtct gagaatccaa gctaacagtt
    10861 gggaaagtta agccgatgac aagtggaata gccagaagag aaatactcgt catcttggct
    10921 aatcggctgt tcagatgact gtgggttttg acttgcttca tccagatata caattgaacg
    10981 atagccaaga taaaagaaag caccatggag atataggcca gataggaata gtgcatgttg
    11041 atgtactggt ttagtttgcc cgacagatgg aggtaaatag tcagttcaaa atagccagct
    11101 aaaactaaaa atcgaatcat aggatgactc caatcaagag agaataggct aaaacgacca
    11161 gagtcacaat cgtaatgaag tgactaataa atcgtgcttt caagtaattt ttcatcataa
    11221 gaatattttt gatatccagc attgggccaa tgacgagaaa agccagaact ggagccaaac
    11281 cgaaactcga gagtagagaa gccccgataa aggcatccgc ctcactacag agcgaaagaa
    11341 gaaaggccaa aatcatcaag agcaggatgg caaaaagagg agtcgcactg atagaggtca
    11401 gaatccgagt cggaacataa acctgtataa cagaggcaaa gagacagcca aataccaaat
    11461 aacgtcccgt atcaaaaaac tcatcaatgg cttgcacaaa gacctgaaaa actttttttg
    11521 caggacttaa gtgagaaaaa tcatgctcat gacaggctag acgattttct ttttgaatcg
    11581 gttcttgcca gaaaaatcct agaaaaattc ctagtattac agccacaacg atggaaccca
    11641 gagctcgtaa tagggcgaca tggaaggagt tgccaaaggc agaataggtc gcaaaaagaa
    11701 caatgggatt gataacaggt gctgtcacaa gaaaaggaac ggccgtgtaa cttggaacct
    11761 ttttttccag aaaacgattg atgatgggga cgattccaca ttcacaggaa gggaaaagta
    11821 taccgacaaa ggtcccaaaa aagattctcc cccaacgatt tcgagggaga aaatgataaa
    11881 ccttgtcagg tgtgatataa acttcaatca gccctgagac aatacttcct atcagaacga
    11941 agggaagggc ttcaatgata atggagagaa aaatggctcc agtttgaagt acactagatg
    12001 ggagttgttg aaaagcagtc atctagtttt tcttttctgc tttggctttt ttctctttgt
    12061 catcagggaa agtgacttgt ttcaaatcag ggagtttagc aaactcttca aacatcttgt
    12121 ccaagtcgtc tcgttttgta aatttgacag ccataggtgt acctcgattc taaattctac
    12181 ctctattata ctattatttt gcggaaaagg gagtttttta atttgttttc gatagttgat
    12241 actcttcgaa aatctcttca aaccacgtca gcttcgcctt accgtagtac ggttactgac
    12301 ttcgtcagtt ctatccacaa cctcaaaaca gtattttgag cagcctacgg ctagcttcct
    12361 agtttgcttt ttgattttta ttgagtatga aaaggattgg agaaagcctt ccagcttgtg
    12421 gtataataga tttatggata aaaaatatga aaaaatctct caggatttgg gagtgacgtt
    12481 aaagcaaatt gataccgttc taagtttgac agctgaaggg gcgactattc cctttatcgc
    12541 gcgttatcgc aaggacatga ctggtagtct ggatgaggtg gcgattaagg ctattattga
    12601 tttggataaa agtctgacaa atctcaatga ccgtaaggaa gctgtcttag ctaagattca
    12661 agaacaaggt aagttgacca aggaattgga agaagctatc ttagttgccg aaaaattagc
    12721 agacgttgaa gaactctatc ttccttataa ggaaaagcgt cgtaccaagg caaccattgc
    12781 ccgtgaagct ggactctttc ctcttgctcg cttgattttg cagaatatag ttgacttaga
    12841 gaaagaagct gaaaagttcg tctgtgaagg atttgcgact ggcaaggaag ccttgaccgg
    12901 tgcagttgat attttggtcg aagccttatc ggaagatgtg accttgcgtt ctatgactta
    12961 tcaggaagtg ctgagacact ctaaactcac ttctcaagcc aaggatgaaa gtcttgatga
    13021 aaagcaggtt tttcagattt attatgattt ttcagagaca gttggaacta tgcaaggcta
    13081 tcgtaccttg gctctcaatc gtggggagaa acttggtgtc ttgaagatcg gttttgaaca
    13141 tgcgacggac cgtattcttg ccttctttgc tactcgtttc aaggtgaaaa atgcttatat
    13201 tgatgaagtt gttcagcaat ccgttaagaa aaaggtcttg cctgctattg agcgtcgtat
    13261 tcggacagaa ttaactgaga aagctgaaga gggagctatc caactttttt ctgacaatct
    13321 gcgcaatctc ctcttggttg ctccactgaa agggcgcgtg gttcttgggt ttgacccagc
    13381 ctttcgtaca ggtgccaagc tagctgtcgt ggatgcaaca ggaaaaatgc tgacaactca
    13441 ggttatttat cctgttaaac cagcatcagc tcgtcaaatc gaagaagcca agaaagattt
    13501 agcagattta attggtcaat acggtgtaga gattattgcc attggaaatg gaacggccag
    13561 tcgtgaaagt gaagcttttg tagcggaagt tctgaaagat ttccctgaag tcagctatgt
    13621 tatcgttaat gaaagtggtg cttctgtcta ttctgccagc gaacttgctc gtcaggagtt
    13681 tccagacttg accgttgaaa aacgctctgc catttctatc gcccgtcgtt tgcaagatcc
    13741 tcttgcggaa ttggtcaaaa tcgatcctaa gtcaattggt gtcggtcaat accaacacga
    13801 tgtcagtcag aagaaactct ctgagagtct ggactttgtt gtcgatacag tggttaacca
    13861 agttggtgtc aatgtcaata cagctagccc agctcttctt tcacacgtag ctggactcaa
    13921 taaaaccatt tctgaaaata tcgtcaaata ccgtgaggaa gagggaaaaa tcacttcacg
    13981 ctcccaaatc aagaaagttc ctcgtttggg ggccaaggct tttgaacagg ctgctggttt
    14041 cctccgtatc cctgaaagta gcaatatcct tgataataca ggagttcatc cagaaaatta
    14101 cgctgccgtt aaggaactct tcaaatgctt ggacattaag gatctgaatg aagaggccca
    14161 aagcaaactc aagtcgcttt cagtcaagga aatggcgcaa gagttagacc ttggtccaga
    14221 aactcttaaa gatatcattg cagatcttct caaaccaggt cgagatttcc gtgattcctt
    14281 tgatgctcca gttctccgtc aagatgtcct agatatcaag gacttagtgg ttggaaaaaa
    14341 gctagaagga gtggtgcgta acgttgttga tttcggtgct tttgttgata tcggaattca
    14401 tgaggacggt ctcatccata tttcccatat gagtcgcaag tttatcaaac accctagtca
    14461 agtggtggca gttggagatt tggtgagcgt ttgggttaag aaaattgata ctgaacgtga
    14521 aaaagttaat ctgtctctcc tagctccaaa tgaaactgac tgattacgtc aagcaggttt
    14581 cactagaaga cttcggcaga ccttttatcc atcatgtcca gtggaatagg cgtctacgtt
    14641 cgacaggtgg gcgatttttc cccaaagatg ggcatttgga ttttaatccc aaggtttatc
    14701 aggaactggg gttggacgtt tttaggaaaa ttgtccgaca tgaactctgt cattatcacc
    14761 tttatttcca agggaagggc tatcaacaca aggatcggga ttttaaggaa cttttgaaag
    14821 cagtggatgg attacggttt gtaccatcct tgcccaatag taactctaaa ccactcaagc
    14881 tttatcgttg tcaatcctgc cagcaaaggt atcagcgcaa gcgtaggatt gataccaaac
    14941 gctatcgctg tggactttgt cgaggtaaat tgcttctgat aaatcagcct gaggactgat
    15001 gaaagccgag cctccccgtg atatactatg tctaacctag cagaaagagg aaatatcatg
    15061 aacagtaaat tttataaaat gagacgcaat cgcatgattt caggagttct ggcaggtctg
    15121 tcagacaagt ggaattttga tgttacccta gttcgtttct tgtttgccat ttttacagtt
    15181 gcaaactttg gacttggcgt gattatctat atcatcctag cttctatcat gcctaccaag
    15241 gaagaaatcg aagcagaaat gtacggaaca ggcccacgta aacgcaaaga agcccaagcc
    15301 attgacgaca atgaaggcag gttttggtga ggttctacag aagatttgga ataagcaaaa
    15361 agctttaaat caatagtcta aactgcttga tttaaagctt ttttactttt atactcaata
    15421 aaaatcaaag tgcaaactag gaagccagcc acaggctgta atttagtcgg caatgtgaag
    15481 ttgacatagt ttgcactttg atttttgaat agtattaata atatttcgcc agcttcatcc
    15541 acaaactata gtggattgga actagaatag tacacctcta cttctaaaac attgttagaa
    15601 atcgatttga ctatcctgat cgatttgtcc tgttcttatt tcattttatt ataaaagcag
    15661 taatttaaac tcagtttttt aatctatttt tgctgctgag acaatacatt tttggaaaaa
    15721 tggtggatgc tgataacaac tgataaccag caaataacat aagcagtgcc ggtaaattct
    15781 gtaccgataa agtgtgtgtg ttcaactaag agctgaccga tgaatggaga gagcagatag
    15841 agaagtccga tgagtatatg ttctttgtgt ttcattaaag cgcaccttct ttccttatag
    15901 attgaacgac ttgcaagtgt taaattgaat ggtatgttat ctaggtaacc ttactaaaat
    15961 gttagttccc gcttcctcct tttcaaagta agccttttca cacttatatt ttacaacttt
    16021 aaaaatagga aatcaaatca ttttctaagt aattttgctt atatttcacc caatcttaca
    16081 aaaatgtaag attaaagtct cttttgtaag gttaggttat ggcaagcggt ctttttttga
    16141 tgtaaactag actcataaag aacaaaggag taacatcatg aaacaaatct tacataagaa
    16201 aactagaaaa ccaagtcaca aagaaattga gcgcgttcag ctgggatgca ctatgatgca
    16261 agctacattt catttgatgg gatattaaga aaggagattc tcatgacact tttagatgta
    16321 aaacacgttc aaaaaattta taaaacacgt tttcagggca accaagtaga agccctcaag
    16381 gatattcact ttaccgtcga aaagggtgac tacgttgcca tcatgggtga gtctggttct
    16441 ggtaaatcaa ctcttctcaa tattctagct atgctggata aaccaagtcg tggtcaggtt
    16501 tacttgaatg gaactgacac cgcaaccatt aaaaattcac aggcctctag cttccgtcgt
    16561 gaaaagttgg gctttgtctt ccaagacttt aacttgctag atactctatc tgtaaaggac
    16621 aatatcttgc ttccgcttgt cttgtcaaga agacctatta cagagatgat gaagaaattg
    16681 gtggtgacag ctgagaatct gggaataaac caattacaag agaagtaccc ttacgagatt
    16741 tctggtggcc agaaacagcg tgtagcagta gcacgcgcca tcatcactga acctgaaatt
    16801 ctccttgcgg acgaaccgac aggagctctt gactccaagt catctgcagc cttacttgat
    16861 gtctttgatg aaatcaatga gcgcggccaa accattctca tggtaaccca ctcaacagca
    16921 gcagctagca gagctaaacg tgtactcttt atcaaagacg gcattcttta caatcaaatc
    16981 taccgtggag agaagacaga acgtcagatg ttccaagaaa tctctgatac cttgactgtc
    17041 atggcaagcg aggtgaatta gtatgtttcg attaaccaat aagttagcgg tattgaactt
    17101 gattaaaaac cgcaaactct actatccttt tgcgctggct gttctcttgg cagtcactct
    17161 cacctatctc ttttactctc taaccttcaa tcctaagatt gcggaaatcc gtggaggaac
    17221 aaccattcag gctacacttg gatttggtat gtttgtcgtc acccttgcgt cagccattat
    17281 cgttctctat gccaatagtt ttgtcatgaa gaaccgttcc aaggaactag gaatttatgg
    17341 catgttgggc ttggagaagc gtcatcttat cagtatgacc tttaaggagt tagtggtatt
    17401 tgggattcta actgttggag cgggtatcgg tattggagcc ttgtttgaca agttaatttt
    17461 cgctttcctg ctcaaactaa tgaaattgaa ggttgagctg gttgctacct tccagacgaa
    17521 agttgtcatt acagtgcttg ttgtcttcgg tttgattttc ctaggcctca tgttcctgaa
    17581 tgcccttcga atcgcccgta tgaatgccct ccagctctct cgtgagaaag ctagtggaga
    17641 gaaaaaaggt cgcttccttc ctctccaaac cattcttggt tccataagtt taggaattgg
    17701 ctattatctt gcccttacgg taaaagatcc tcttacagcc ttaacaacct tcttcatagc
    17761 tgttttactg gttatctttg ggacttatct cttgtttaat gcagggatta ccgttttcct
    17821 ccaaatctta aagaaaaata agaaatacta ttaccaacca aataacctca tatctgtttc
    17881 taacttgatt ttccgtatga agaaaaatgc agttggacta gcaactatcg ctattttgtc
    17941 aacaatggtt ttggtaacca tgtcagcagc gacaagcatt ttcaattccg cagaatcctt
    18001 taaaaaagtt ctaaatcctc atgattttgg ggtttcagga caaaatgttg aaaaagaaga
    18061 tttggacaaa ctcttgagcc agtttgcaag tgacaatggt tataagatta aagaaaaaga
    18121 agtgtttcgt tacacttact ttggtgttgc gaaccaagaa ggaaataagt taaccttttt
    18181 tgaaaaagga caaaatcgtg tccaacccac aacagttttc atggtatttg accaaaaaga
    18241 ttatgaaaat atgactggtc aaaaactgtc tctatcagga aatgaggtcg gtctctttgc
    18301 caaaaatgac ggactgaaag gacagaaagc tctaactcta aatgatcatc aattttctgt
    18361 caaagaagaa tttaataaag atttcattgt caaccatgtt ccaaataagt ttaatatctt
    18421 gactactgat tacaattacc ttgttgttcc tgatttacaa gcctttttgg atcaattccc
    18481 agattcggct atctataatc agttttacgg tggtatgaat gtagatgcca gcgaagaaga
    18541 acaactcaag gtcgctgagg agtatgaaaa ctacctcaat caatttaatg ctcaattaga
    18601 cacagaaggt agctatgttt atggtagcaa tctagcagat gctagttctc agatgagtgc
    18661 cctctttggt ggtgtcttct ttatcggtat tttcctatcc attatcttta tggtcggaac
    18721 tgttctggtc atctactaca aacaaatttc tgaaggctac gaagaccgtg aacgctttat
    18781 tatcttgcag aaagtcggtt tggaccaaaa gcaaatcaag caaaccatca acaaacaggt
    18841 tttaactgtt ttcttccttc ctttgctctt tgccttcata catctcgcct ttgcctacca
    18901 tatgcttagc ctgattttaa aagtgattgg tttactggat acgactatga tgttgattgt
    18961 gaccttgtct atctgcgcta tcttcctcat cgcctatgtg ctgattttca tgattacttc
    19021 aagaagttat cgcaagattg tgcaaatgta aaaaagatac ctcgacttca aaatcgaggt
    19081 atttcttgta ttctaaatgc tgaaaagttg ttcgagcagg aaggtaactc ccatggtcaa
    19141 gagaccaata gcaaggttcc gaatcatagc tgttctagtc ggatcctttc caagtctggc
    19201 actagtgtaa ccagtgagaa gaagggccac accgacaata aggacagtag cagggatgcg
    19261 atattcactt gggaatatgg tcactgacag cattggaggc aaacttctaa ggaaaaaggc
    19321 aacgaagcta gaaatggcag cgtgccaagg attggtaaat tcttcatact caatcccata
    19381 tttttcctct accagagcct tgagtggatt tttaagaaag atcttattgg tcaagagttg
    19441 ggcagaagtt ttgcattctc cattttggat ataagcagca tagagggatt ttttggctag
    19501 ttccctatct tggtctaaca agagtttttc tcgcgaaacg gcagcttcct cggtatcttt
    19561 tggagttgaa acggatacat attctccacc agccattgaa aaggcaccag ctaagatagc
    19621 cgcaaatcct gataaaaaga taatccagat attggtcgtg gcactggcaa ccccgataac
    19681 cacaccagca atggaaataa ttccatcgtt agcaccaaga acacctgcgc gcaggatatt
    19741 taaacgacct gcaaaatttg aatcgatttc gtgatttgtt tctgacgcta aatttcaagt
    19801 tcaagttagc catccagaag tcttctctgg gtgacttgta gtccaagcat tttttaggat
    19861 agttgttaat ccacttttcg atgaatgcga cttctttggg agtcattttc ttggttccct
    19921 taggtaacca tctacgaatg agcctgttgt gattctcatt agttcccctt tcccaagagg
    19981 cataggggtg cgcataatag atgtgctcct cagaaaatac atcaaacaag cgattgaatt
    20041 ccgttccatt atctgccgtg atggaaagaa tcttgtgttg ttttaagatg agttttagag
    20101 cctgattgac cacctcagca cttttatttg gaatcaatcg gatgatctga tgtctactct
    20161 ttcgatccgt caagacaagc aagcagtagt ttttcgctct cgtaagtaga accgtatcaa
    20221 tctcataatg cccattctcc aagcgaagat tgatagcttc agaccgctgt tcgatagatt
    20281 gaccagcagg tttaaagttg gtgctagcct gtttcttaag cgcttttcct tttctagggt
    20341 aaggcaaatc ctgcttgctt aaccccaatt ttccatgatg aatccaatag taaatggttg
    20401 aaattcccac gttaacccct ttagccataa ccatcatttc aggcgaaaat ttttggttat
    20461 gatagtggag aatcttttcc tttagttcct tggtcaagct tgatttcttg accgagcgct
    20521 tgcgattgtt ttcataagac tgttgagcat agtcggcaga ataaacctct ttgaagcgcc
    20581 cttttccaag acattgtcgg actgtcccac gcttgatttc agtgtggata gtttgaggaa
    20641 cttttccaag cagagaggca atttctctat ttgatttccc tttgttcgcc ttttgtagta
    20701 taatggtctt gcatctctgc gcctttcttg tgtttgtggt tgaacaacaa gtataacaca
    20761 gaggtgtttt cttatgccta caagagctat cggctagttg aaccatctaa tttttaggag
    20821 ggctgggtgg ctaacttcat tatagaactt tcatttacga acatatagta aaatgaaaca
    20881 agaacagaac aaatcgatca ggacagtaaa atctatttct aacaatgttt tagaagcaga
    20941 ggtgtactat tctagtttca atctactata tttttatttt ttatcaaaaa atactttaca
    21001 agttcttaaa aacatggtat agtaataaag cttagaaaat gagatgatgt tttctagcaa
    21061 atataaaccc gagtaaaaaa tgcctacgga caggcagggt tgaatgccga agcgtggttg
    21121 aaaagccaca ttattgatag ggttaaaagc ctacttttat aagttgatgt taggacactt
    21181 gttctaattc ataaattttt agtgtggtga aagcacacgt catcttgtga aacgatcaat
    21241 aaagtacgta atatttgcta ctagagagtt aggaaacatc gggaacagac atactcaaca
    21301 gaaaccaaaa taaacacgtc agaagattgc agagcaggtg aaaacctgct cttttttcat
    21361 gagtcaacct ttagttcctt agttttcata aggtcctaaa aatattgaaa ggagtatgtt
    21421 ttgaaagagt tagatcaaaa ccaagcccca atttatgagg ccttggtgaa gttacgcaag
    21481 aaaaggattg ttccctttga tgttccaggt cacaagcgtg gacggggaaa tccagaactt
    21541 gtcgaactct taggagaaaa atgtgtaggc attgatgtca attcgatgaa acccttggat
    21601 aatttaggcc atcctatttc gattattcgt gatgcagagg agctggctgc agatgctttt
    21661 ggagctagcc atgcctttct aatgattggt ggaacaactt catcggtgca gactatgatt
    21721 ctggcaacct gcaaggcagg agataagatt attctgccac gaaatgtcca taaatctgct
    21781 atcaatgcgt tggttctatg tggtgccatt cccatctata tcgagatgag tgtagatcct
    21841 aagattggta tcgctttagg tcttgaaaat gaccgagtag cacaggccat aaaggaccat
    21901 ccagatgcta aggctatcct aatcaacaat cctacttact acggcatctg ttcagaccta
    21961 aaggggttga cagaaatggc tcatgaagct ggcatgatgg ttttagtaga tgaagcccat
    22021 ggagcgcatt tgcatttcac tggtaaactt ccaatttctg ctatggatgc aggagctgat
    22081 atggcagcag tttccatgca taagtctggt gggagtttga cacaaagttc gcttctttta
    22141 atcggggagc agatgaatcc cgagtacgtt cgtcagatta taaacctgac ccagtctaca
    22201 tctgcctctt acttattgat ggctagtttg gatatttcac gtcgcaactt ggcccttcgt
    22261 ggtaaagagt cgtttgagga agtcattgag ctatccgagt acgctcgtca tgaaatcaat
    22321 accattggcg gttactatgc ttactcaaaa gagttaatag atggtgtgtc ggtctgtgat
    22381 tttgatgtaa ccaagttgtc agtttacact cagggaatag gcttaacagg tatcgaggtt
    22441 tatgacctct tgcgagacga atacgacatt cagatcgagt ttggtgatat cggcaatatc
    22501 ttggcctata tttccatcgg cgaccgcatc caagacatcg agcgcttggt tggtgctctg
    22561 gctgatatta agagactcta ttcaagagat ggaaaagatt tgatagcagg agaatatatt
    22621 cagcccgagt tagtgctgtc tccgcaagaa gccttctatt cagagagaaa aagtttgacc
    22681 ttggacgaat ctgttggaca ggtctgtggg gaatttgtca tgtgttaccc tccaggaatc
    22741 cctatcttgg ctcctggcga acgcatcaca cgagaaattg tcgactatat ccaatttgcc
    22801 aaggaacgtg gttgctccct ccaagggacg gaagatccag aggtcaatca catcaacgtc
    22861 attaagagaa agacaaacta taagaaaagt caatagtttt atctaaacta tttcttattt
    22921 caatttgatg atttggcgat gattttagag catggcaaaa agctcttgaa aaagtccatt
    22981 ttttcaaagg taatcctgtg ttaatttcag aaattacatc actttttg
//
LOCUS       DAVKJJ010000034        22803 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000034 DAVKJJ010000000
VERSION     DAVKJJ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22803
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.034"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            110..763
                     /locus_tag="VW144_001349"
     CDS             110..763
                     /locus_tag="VW144_001349"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU9600792.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANTFLKETGQEPIDWLR"
     gene            773..1237
                     /locus_tag="VW144_001350"
     CDS             773..1237
                     /locus_tag="VW144_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9600793.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAVREILEETGLKAKPVLKGVITFPEFTPELDWYTYVFKVTEFEGDLIDCNEGM
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1250..2518
                     /locus_tag="VW144_001351"
     CDS             1250..2518
                     /locus_tag="VW144_001351"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU9600794.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGHVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFKAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2532..2792
                     /locus_tag="VW144_001352"
     CDS             2532..2792
                     /locus_tag="VW144_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600795.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2803..4164
                     /gene="pdrM"
                     /locus_tag="VW144_001353"
     CDS             2803..4164
                     /gene="pdrM"
                     /locus_tag="VW144_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEU9600796.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDNIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4318..5286
                     /locus_tag="VW144_001354"
     CDS             4318..5286
                     /locus_tag="VW144_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU9600797.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLAFNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNVEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5302..6294
                     /locus_tag="VW144_001355"
     CDS             5302..6294
                     /locus_tag="VW144_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU9600798.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            6512..7555
                     /locus_tag="VW144_001356"
     CDS             6512..7555
                     /locus_tag="VW144_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEU9600799.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7619..9304
                     /gene="lpdA"
                     /locus_tag="VW144_001357"
     CDS             7619..9304
                     /gene="lpdA"
                     /locus_tag="VW144_001357"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU9600800.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHSAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVAVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9368..10357
                     /locus_tag="VW144_001358"
     CDS             9368..10357
                     /locus_tag="VW144_001358"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEU9600801.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEAVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10430..11500)
                     /gene="xerS"
                     /locus_tag="VW144_001359"
     CDS             complement(10430..11500)
                     /gene="xerS"
                     /locus_tag="VW144_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEU9600802.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
     gene            complement(12084..13634)
                     /locus_tag="VW144_001360"
     CDS             complement(12084..13634)
                     /locus_tag="VW144_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU9600803.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLAMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALIGAICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(13650..14429)
                     /locus_tag="VW144_001361"
     CDS             complement(13650..14429)
                     /locus_tag="VW144_001361"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU9600804.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGHGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(14416..15267)
                     /gene="ylqF"
                     /locus_tag="VW144_001362"
     CDS             complement(14416..15267)
                     /gene="ylqF"
                     /locus_tag="VW144_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEU9600805.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LIKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            complement(15474..15650)
                     /locus_tag="VW144_001363"
     CDS             complement(15474..15650)
                     /locus_tag="VW144_001363"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9600806.1"
                     /translation="MQAGIPTDNEVSYALIYQQETLPATGSSTSALTALGLLAVGSLV
                     LLVHNMTGTVFCSL"
     gene            complement(15702..21329)
                     /locus_tag="VW144_001364"
     CDS             complement(15702..21329)
                     /locus_tag="VW144_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9600807.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGEPAVQPELPEAVVTDKG
                     ETEVQPESPDTVVSDKGEPKQVAPLPEYTGPQASAIVEPEQVAPLPEYTGVQAGAIVE
                     PAVQPALPEAVVSDKGVPEVQPELPEAVVSDKGVPEVQPELPEAVVSDKGEPEQVAPL
                     PEYKGNIEQVKPETPVEKLQEEEPEKTIELRSVSDVELYSYEANQSKQHTILDKVPDN
                     KSAYFIKVKSAKFKDVLLPVSNIEDTTKDGKVVYKITARLEHLKQSVDNQYQDNFTYY
                     LPKVVQNDGPIYTSFKKLVTDMNSRPDGTFILGATMNAREFELGENQESYVTKNFTGT
                     LIGSHQGKHYAIYNLKKPLFNTLNNAAIQDLTLKDVNISGKNHVASVAMEATNSSTLD
                     NVHANGIIAGELGIGGLVQKVDNSTVRNSSFTGRITNTFVTTSQYAIGGLVGQLTGAN
                     ARVAHSISTVVISSNGEHSNQTIGGIVGIVENNATVENSYAEGEINNAKPFARVGGVA
                     GSLWTQEGGTDNNTGRLTNVLSDMNITNGNAISGYHFNGIKETNVYSNKENKVVNVQA
                     VGDEVMSVDSTVERGTEIEKTSVQAKKDEITKKHTITVEDFTNSVKQDVDYTKAAHYQ
                     STREVAYKNIEKLLPFYNRDTIVKYGNQLSTDHNLFKKELLSVVAMKNDEVVSDITSN
                     KNSINHLLLHYKDGTSEKVDLTYNSDFANLAEYNIENTGLVYTPNAFLKNYTPIIDRV
                     KNDLTAVPYNPTALKNLLGISDNVKLTELYLDEQFTKTKEHLTETLKKLLSADAAVSG
                     NNDIIDNYIVDKIKRNKEALMLGLTYLERWYDFKFDKASAKDLLMFHMDFFGKGNTSP
                     LDAIIELGKSGYNNLLAKNNVVTYNALLTNNYGTKDLFSALEGYRKAFAPHQTNNEWF
                     KSQTKAYIVEEKSNIEEVKTKQGLVGTKYSIGVYDRITSATWKYRNMVLPLLTLPEKS
                     VFVISTISSLGFGAYDRYRNSEHKAGKDLNDFVEENARETAKRQRDHYDYWYRILDDN
                     AREKLYRNILLYDAYKFGDDNTVGKATEVADFDNPNPAMQHFFGPVGNKVGHNQHGAY
                     ATGDAVYYMGYRMLDKDGAITYTHEMTHDSDQDIYLGGYGRRSGLGPEFFAKGLLQAP
                     DHPDDATITINSILKHSKSDSTESRRLQVLDPTTRFNNADDLKQYVHNMFDVVYMLEY
                     LEGNSILKLDTNQKQQLLRKVTNEYHPDPDGNKVYATNVVRNITVEEVERLRSFNDLI
                     DNNILSSREYASGKYERNGYFTIKLFAPIYAALSNDDGTPGDLMGRRMAYELLAAKGF
                     KDGMVPYISNQFEADARANNKTITSYGKTKGLVTDTLVLQKLFNGQYNTWSDFKKAMY
                     KERQDKFNKLNKISFKDPSQPWTRNIIKTIHSVNELQNLMNEAVRKDTETPHWYNYNP
                     EIDSAVHKLKRAIFKAYLDQTNDFRSSIFENKK"
     gene            complement(21538..22716)
                     /locus_tag="VW144_001365"
     CDS             complement(21538..22716)
                     /locus_tag="VW144_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600808.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLMFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLGRLVI
                     "
BASE COUNT         6581 a         4412 c         4602 g         7208 t
ORIGIN      
        1 attcaaatcg tatatagtcg atttattaaa tctttagata aactgtaaaa agtggtttcc
       61 atagccactt tttgctataa tagaggcagt aaaaatgaag gagtcggcta tggaacactc
      121 gtcttggcat gctttgatta aggcgcaatt acctgagggt tatttcggga aaatcaatca
      181 gtttatggag caggtctatt ctcaggggat tatttatcca cccaaggaaa aggtttttca
      241 ggctctcttg acaacactgc ttgaagaagt taaggtggta attctagggc aagaccccta
      301 tcacggacca ggtcaagcgc agggcttgag tttttccgta cctgactcta tcccagctcc
      361 accatccttg caaaatatct tgaaagaatt gtcagatgat atcggagtta agaaatctca
      421 tgatttgaca gcttgggctg agcaaggagt cttgcttctt aatgcttgtt tgactgttcc
      481 tgctggacag gccaatggtc atgctggtca aatctgggag ccttttactg atgctgtgat
      541 tcaggtggtc aatcatctag atagaccagt cgtttttgta ctctggggag cttatgcacg
      601 taagaagaag gccttagtta ccaatcctca tcacttgatt attgaatcag cccatccaag
      661 tcctttgtcg gtttatagag gattttgggg ttccaagcct ttttccaagg ccaatacatt
      721 cttaaaagag acaggacaag agccaatcga ttggcttaga taaggagaaa atatgcctca
      781 gttagcgacg atttgttaca ttgataatgg gaaagaactg ctcatgctcc atcgtaataa
      841 gaaacccaat gatgtccatg aagggaaatg gattggtgtg ggtggtaagc tagagagagg
      901 agagacgccc caggaatgcg cggtacgtga aatccttgaa gaaacagggc tcaaagccaa
      961 gccagttcta aaaggtgtca tcacttttcc tgaatttaca ccagaattag actggtacac
     1021 ctatgttttt aaggtgacgg agtttgaggg cgacttgatt gactgcaatg aggggatgct
     1081 agaatgggtt ccctatgatg aggttttgag caagccgact tgggaaggtg accacacctt
     1141 tgttgagtgg cttttagagg ataaaccctt cttttcagcc aagtttgttt atgatgggga
     1201 taaattgttg gatacccaag ttgatttcta tgaataaagg agaaagcaga tgctactaat
     1261 caaaaatggt cgtgtaatgg atcccaagtc tggtttggat caagtttgtg atgtcttagt
     1321 tcaagatggg aaaattatca aaattgcgcc tgagatcacg gaagaaggag cagaaacgat
     1381 tgatgctact ggtcatgtgg ttgctcctgg tttggtcgat attcatgtgc atttccgtga
     1441 acctggtcaa acacataaag aagacattca tactggtgcc ctagcagccg ctgcaggtgg
     1501 ttttactact gtcgtcatga tggctaatac cagtccaacc atttcagacg tggagacttt
     1561 gcaagcagtt ctccagtcag ctgccaaaga gaagattaat gtcaagacag ttgcgaccat
     1621 tactaaaaac tttaatggta aaaacttgac tgactttaag gcacttttag aagctggtgc
     1681 ggttggtttc tctgatgacg gtattccgct tgagagcagt aagattgtca aggaagccat
     1741 ggaggaagcc aaaaaactca atacctttat cagccttcat gaggaagatc caggtttgaa
     1801 cggtgttctt ggctttaatg aaaatattgc tagagaacat ttccatatct gtggtgctac
     1861 tggggtggct gagtacgcta tgatggcgcg tgatgtcatg attgcctatg caactaaagc
     1921 ccatgttcac atccagcatt tgtctaagga agaaagtgtt aaagtagtgg agtttgctca
     1981 ggggttaggt gcagaagtca cagcagaagt agcaccacag catttctcta agacagaagc
     2041 acttctttta acacaaggta gcaatgctaa gatgaatcca ccgcttcgtt tggaatcaga
     2101 ccgtcgtgcc gttatcgaag gtctcaaatc aggtgtcatc acagttattg cgactgacca
     2161 cgcgcctcat catgtagatg aaaaaaatgt tgaagatatt accaaagcgc catctggtat
     2221 gactggctta gaaacatccc tgtctctcgg cttgacctat ttggtagaag ctggtgagtt
     2281 gagcttgatg gaattacttg aaaaaatgac atacaaccca gccaagcttt acaactttaa
     2341 agcaggttac ttggctgaga atggtccagc agatatcact atttttgatg ccaaggctga
     2401 ccgccttgtg gactcccatt ttgcttccaa agcagctaat tcaccattca tcggtgaaac
     2461 cttaaaaggg caggttaaat ataccatctg taagggacaa atcgtctatc aagcttgata
     2521 ggagttagaa tatgaattat tttcgaaaac gtagggagag acaagcgaaa tctaatagtg
     2581 gaatttactg tcccgcagct aatcgttcca aaattcaata tgaaactaaa gaaaaagctg
     2641 accatgcggt tcaatatgat aacggaggtt tagttagaag ctattattgt cgtacatgtg
     2701 cgtgttggca tactacttca aaatctaata aacctccgct aagtttgaaa aactatggtc
     2761 tgaaactctt tggtttagat aaatcagaat agaaagagaa gtatgtataa gacaaagtgt
     2821 ttacgagaga agttagtatt atttttaaaa attttcttcc caatcctgat ctaccaattt
     2881 gccaattatt ctgcctcttt tgttgatact gcaatgacag gtcaatacaa cactatggac
     2941 ttggctggtg tatctatggc aaccagtatc tggaatcctt tctttacatt tctaacaggg
     3001 attgtgtcag ccttggtgcc tatcattggt caccatcttg gtcgaggcaa aaaggaagaa
     3061 gttgcgtctg atttttacca atttatttat ttggccttgg gcctatctgt ggtcttgctg
     3121 gggatggtac ttttcttggc accaacaatc ttgaatcata ttgggttaga agcagcagta
     3181 gcggcagtag cggttcgcta tctttggttt ttatctatcg ggattatccc cttgttgctc
     3241 tttagcgtca ttcgttcctt gctggattcg ctgggcttga ccaaactgtc catgtacctc
     3301 atgcttttgt tactccctct caatagcgga tttaactatc tcttgattta cggtgccttt
     3361 ggtgttccag aactgggagg ggctggtgct ggtttaggaa catccttggc ctactgggtc
     3421 ttgcttggga tttctgttct ggttttattt aaacaggaga agctcaaagc cttacacctt
     3481 gagaaacgaa ttccacttaa tatggataac attaaggaag gagttcgttt aggtctgcct
     3541 attgggggaa ctgtcttcgc ggaagtggct atcttttcag tggttggctt gattatggct
     3601 aagttttcgt ccttgattat agctagtcac cagtcagcta tgaacttttc aagtcttatg
     3661 tacgcctttc ctatgagtat ctcatcggct atggctattg tcgtttccta tgaagtggga
     3721 gccaagcgat ttgatgatgc gaaaacctat attggtctag gaagatggac tgctctcatt
     3781 tttgcggcct tcaccctaac cttcctttac atttttaggg gaaatgtggc cagtctttat
     3841 ggtaacgacc caaaatttat cgatttgaca gcgcgttttt taacttatag tcttttcttc
     3901 cagttagcag atacctttgc ggcaccgctt cagggaattt tgcgggggta taaggataca
     3961 gttattcctt tttaccttgg tttgcttggt tattggggcg tagcaatccc tgtggctacg
     4021 ctatttgatt ccctaacaga ttttggagct tattcttact ggatcggctt gattattagt
     4081 ttgattgtga gtggggcgct ctaccgttgg cgtttaactg tgattatgaa gagatttgaa
     4141 tctttagcaa aatccaaacg ctaaaaagtt tgtgaaaaaa tatccttgac aagaaaatcc
     4201 cttgttttca cggggttttc ttttttattt tgtgaaattt cttttagaaa aactttgaca
     4261 gtatttgcca aaagcggtaa aatagtaagg taagaagaaa gttagaaagg caagaagatg
     4321 tcaactttag ataaaaatct tttgctagaa atgttccgta agatggaaga aatccgtcgc
     4381 atggacttaa aaattgcaca attagtaaag aaagggaaag tgccaggaat gacgcacttt
     4441 tctgttggtg aagaggcagc taacgtgggg gctatgttag ctttcaatcc agatgatctg
     4501 attacctcaa accaccgtgg acacgggcaa gctattgcta aagggattga cctcaacgga
     4561 atgatggctg aaatccttgg taaatacact ggaacctgta aagggaaagg tggatctatg
     4621 catatcgctg accttgatgc tgggaacctt ggtgccaatg gtatcgtagg tggtggtatg
     4681 gggatcgctg tcggtgcagc cctcagtcag caaatgcaaa ataccggtaa aatcgttgtc
     4741 tgcttctttg gagatggtgc gaccaatgaa ggtgttttcc acgaagcagt gaacatggct
     4801 tctatctgga acctgccagt cattttctat tgcattaaca acggttacgg tatctctgcg
     4861 gatatcaaga aaatgaccaa tgtagaacat atccatcaac gtagcgccgc ttatggaatt
     4921 cctggaatgt tcatcgaaga cggtaacaat gtcatcgatg tctatgaagg atttcagaaa
     4981 gctgtagacc atgttcgcag tggcaatggt ccagtcttga ttgaaagtgt aacttatcgc
     5041 tggcttggtc actcatcatc tgaccctggt aaatatcgta cgcgtgaaga agtggaattg
     5101 tggaaacaaa aagatccaat cgaaaacctc cgcaattacc ttattgaaaa taacattgca
     5161 agtgccgaag aattggaaga aatccaagcg caagtaaagg aagcagtaga agcttctgtt
     5221 aaatttgcag aggaaagccc attcccaccg cttgaatcag cctttgaaga tatttacgca
     5281 gactaaggag aaagagataa aatggaaaca aaaacaatgt ccttccgtga caccattatc
     5341 cttgctatgt ctgaggaaat gcgtcgcgat gaaaatgtgt tcttgatggg agaagacgtc
     5401 ggtgtcttcg gaggagactt cggtacttct gttggaatgc ttgaagaatt tggtccagaa
     5461 cgtgtccgtg actgtccgat ttctgaagct gccatctctg gagcagcagc aggagcagcc
     5521 atgacaggac ttcgtccaat cgtcgatatg accttcatgg acttctctgt tattgccatg
     5581 gacaatatcg tcaaccaagc tgctaaaaca cgttacatgt ttggtggtaa aggtcaggtt
     5641 ccaatgactg ttcgttgtgc agctggtaac ggagttggct ctgccgccca gcactcacaa
     5701 tctctagagt cttggtttac tcacattcca ggacttaagg ttgtggcacc tggaacacct
     5761 gcggacatga aaggcctgct taagtcttct atccgtgata acaacccagt tattatactt
     5821 gagtacaagt cagaatttaa ccaaaaaggg gaagtgccag ttgatccaga ctacacaatt
     5881 ccacttgggg ttggtgaaat caaacgccaa ggaacggatg taacagttgt tacttatgga
     5941 aaaatgcttc gccgtgtggt tcaagctgct gaagaattag cagaagaagg aatttcagtt
     6001 gaaattgttg acccacgtac ccttgttccg cttgataagg atatcatcat aaactcagtg
     6061 aagaagactg gtaaggttgt tctggtcaac gatgcccaca aaacaagtgg ctatatcggt
     6121 gaaatttcag ctattatttc agaatcagaa gcatttgact atctagatgc accaatccgc
     6181 cgttgtgcag gagaagatgt gccgatgcct tatgcacaaa acctagaaaa tgcaatgatt
     6241 ccaacagttg aaagtatcaa agatgcaatc cgaaaaactt ataacaaaga atagagttac
     6301 ataagataag ttatgtaaat aaaataaagc tgacgagaaa ctttgtatct tttaggtgca
     6361 gagctcttcg tcattaatat cttagattag agcacgggct aaaagctcgg aaaaaagata
     6421 aatctccttg gcttcatcga agccatcgtc gatttcctat ttttcagtcg cttttgacgc
     6481 cctttgtatc ataaatagaa ttggagaggt catggctgat gacaagctaa gagcgactcc
     6541 tgcagctaga aagttagcgg atgatttagg gataaacctc tacgacgttt ctggctcagg
     6601 tgcaaacggt cgtgtccaca aagaagacgt ggaaacttat aaagacacaa acgtggttcg
     6661 catttcgcca cttgcaaaac gaattgccct cgaacataac attgcttggc aggaaatcca
     6721 aggaaccggt catcgtggta aaatcatgaa gaaggatgtt ttggccctgc ttcctgaaaa
     6781 tatcgaaaac gatagcatca aatctcctgc tcagattgaa aaagtggaag aagtccctga
     6841 taacgtaaca ccatatggtg aaatcgagcg tattccaatg acaccaatgc gtaaggttat
     6901 tgcccaacgc atggttgaat cttacttgac tgcgccaacc ttcaccctca actatgaagt
     6961 tgatatgact gaaatgttgg ctcttcgtaa gaaggttctt gagccaatca tggaagcaac
     7021 tgggaagaag actactgtaa cagaccttct ttcgcttgca gttgttaaga ctcttatgaa
     7081 acacccatac atcaatgctt cattgacaga agatggcaag actattatca ctcacaacta
     7141 tgtcaatctt gccatggcag ttgggatgga taatggattg atgacacctg ttgtttacaa
     7201 tgctgagaag atgagtcttt cagaactggt tgtagccttt aaggatgtta ttggccgtac
     7261 cttggatggt aaattggctc caagtgagtt acaaaattca acattcacaa tcagtaattt
     7321 gggaatgttt ggtgttcagt cctttggccc gattattaac caacccaact cagctatcct
     7381 tggtgtcagt tcgacaatcg agaagccagt tgtcgtcaat ggtgaaattg tgattcgccc
     7441 aatcatgagt ttaggattaa ccattgacca ccgtgtcgta gatggtatgg ctggtgctaa
     7501 gtttatgaag gacttgaaag agttgattga aaccccaatc tcaatgttga tttaagagca
     7561 agagtattca tttttaagat atagataaag gaaggaagac atggccttag aagtaattat
     7621 gccaaaagcc ggcgtggata tgacagaagg acaaatcgtc caatggaata aaaaagtcgg
     7681 agaatttgta aaagaaggag aaatcctttt ggaaatcatg actgataaag tcagcatgga
     7741 attggaagcc gaagaagatg ggtacttgat tgccattctc aaaggagatg gtgaaactgt
     7801 ccctgtaacg gaagttatcg gttaccttgg tgaagaaagg gaaaacatcc caacagctgg
     7861 agcagcatcg ccagaagcta gccctgtacc tgtagcaagt acctcaaacg atgatggtaa
     7921 gagcgatgat gcttttgata tcgttgtgat tggtggaggt cctgctggtt atgttgcagc
     7981 cattaaagct gcccaactcg gcggtaaggt tgcccttgtt gagaaatctg aacttggtgg
     8041 aacctgctta aaccgtggat gtattccaac caagacctac cttcatagcg ctgaaattat
     8101 tgaaaatatc ggtcatgctg caaatcgtgg tatcgtgatt gaaaatccta acttcactgt
     8161 tgatatggaa aaacttttag aaactaaatc taaagttgtt aatactcttg ttggtggtgt
     8221 tgcaggactt cttcgtagct acggagttgc tgttcataaa ggaattggta caatcactaa
     8281 agacaagaac gtcttggtaa atggttctga attgcttgaa accaagaaaa tcattcttgc
     8341 tggtggttca aaagtcagca agatcaacgt ccctggtatg gaatctccac ttgtcatgac
     8401 tagtgatgac attcttgaaa tgaacgaagt gccagaaagc cttgttatca tcggtggtgg
     8461 agttgtcggt atcgaactcg gtcaggcctt catgacattt ggttcaaaag tgactgttat
     8521 cgaaatgatg gaccgtatag ttccagctat ggatgtggaa gtttctaaga accttcgctt
     8581 gatccttgag cgtaaaggaa tgaccatctt gactggtact aaactgcaag aaatcattga
     8641 ggaaaatggt caacttcgta tcaaggttga aggaaaagac gatatcatcg caagcaaagc
     8701 tcttctttca attggtcgta tgccagacct tgaaggtatt ggagaggttg agtttgaatt
     8761 ggatcgtggt tgtatcaagg tcaacgaata catggaaact tcagttccag gcatttatgc
     8821 accaggtgac atcaacggta ctaagatgtt ggctcacgca gctttccgca tgggtgaagt
     8881 ttccgctgaa aatgccctta aaggaaatca tgcagttgcc aaattgaatt tgactcctgc
     8941 agccatctac actcttcctg aagtagcagc agtaggtttg acagaagaac aagcccgtga
     9001 gaaatacgat gttgccatcg gtaagtttaa ctttgctgct aacggtcgtg ctattgcatc
     9061 tgacgcagct caaggtttcg taaaagttat cgctgataag aaatacggag aaatccttgg
     9121 tgtacacatt attggtcctg cagccgcaga attaataaac gaagcatcaa gcatcatcga
     9181 aatggaaatc actgttgagg aaatgctgaa gaccatccac gggcacccaa cttactctga
     9241 agtgatgtac gaagcgtttg cagatgttct aggaatggcc atccattcac ctaagaaaaa
     9301 ataaaacgaa gatagattag actggaaggt tatttccagt ttctatcata taaagtaaag
     9361 ggatatcatg aaatacatta tcaatcattc aaatgacact gcttttaata ttgccttgga
     9421 agaatacgcc tttaaacacc ttttggatga ggatcaaatc ttcctacttt ggattaacaa
     9481 gccatctatc attgttggtc gtcaccagaa cactatcgaa gaaatcaacc gtgattatgt
     9541 tcgcgaaaat ggtattgagg tagttcgccg tattagtggt ggtggagctg tttaccacga
     9601 tttaaataac ctcaactaca cgatcatctc caaagaagat gaaaataagg catttgactt
     9661 caagagcttc tcaactccgg ttatcaatac cctggctcaa ctcggggtta aagctgagtt
     9721 cacaggtcgt aatgaccttg agattgatgg taagaagttc tgtggcaatg cccaagccta
     9781 tatcaacggc cgtatcatgc accacggttg cttgctcttt gacgttgatt tgtcagtcct
     9841 cgcaaatgcc cttaaggttt caaaagataa atttgaatca aaaggtgtga aatccgtccg
     9901 tgcccgtgta accaatatta tcaatgaatt accaaaaaaa atcacagtcg aaaaattccg
     9961 tgatttactc ttggaataca tgaaaaaaga gtacccagag atgactgaat acgtcttttc
    10021 agaagaagaa ttggccgaaa taaatcgcat caaggatact aagtttggaa cttgggactg
    10081 gaactatggt aaatcacctg aatttaacgt ccgtcgtgga accaaattca ctagtggtaa
    10141 ggttgaagtc tttgctaacg ttactgaatc aaaaatccaa gacatcaaga tttatggtga
    10201 cttctttggt attgaagacg ttgctgcagt agaagctgtc cttcgtgggg tgaaatacga
    10261 acgcgaagat gttcttaagg cgctaaaaac cattgatatc acacgctact tcgctggtat
    10321 tagccgtgaa gaaatcgctg aagcggtagt tggataaatc aaaaagaagt tggccaacta
    10381 accaacttct ttttttgata tttatttaca taatttaaaa tacgtaaaat cataaactat
    10441 ccagagcatt cttttgttca tcactaacaa tatgggtata gaggtcagtg acttgtgtgc
    10501 tggcatgtcc tagttggtga ctgactaaaa cttgtgattt agtcgcatca tagagcctag
    10561 ttgctagtgt atggcgcagt ttatggggtg ttacacgcac tttaaaatcc tctgagtatt
    10621 tagcaaccat tttctcaacg ctagaagcat cgatacgatt aggaacacct ctgtagagag
    10681 ttaaaaaaag ggctgtatct gttttttccg ttttatagcg ttgattccga atggccagat
    10741 aattctctaa ataaggttta gcaaaagcag caacattgac tgagtcacgt ttgccacctt
    10801 ttcgagtaac atcaataacc atcattttta gattgagatc tcttagatct agattaacag
    10861 cttcagataa gcgaacacca gatgccaaga gaagggcaat aatggctaaa tcacgttctt
    10921 tatttttgtt gaatgatgag agagctcgat ttgaaagctg ttgtgggtgc tcttgatcga
    10981 tataagttag aaaaccttct gtttcatcac ctagaaagag tttttgcttg atattttcag
    11041 ctctggcagc aagggtttct ttctttttct tggttgaaac ttttttcatt acattacgat
    11101 agaaataagg ttccccctga tcgttttcaa cctcctcggt tagatacttg taaagactag
    11161 aaagtgctga taaggttcga ttgatagttg tctgtgaaac accttgtttt gttgtattag
    11221 cattcagcaa gggacgttca cgtagataaa ggataaagga ttccatgtct ttcttagaca
    11281 tattttccaa aactgataaa ggaatatcgg atattttatc agcgtttgaa ataccagact
    11341 ccaaaaccca gctgaaaaat cggtcatatt ccttaaggta ttcgtacagg gttgtaaaac
    11401 tgtagggtac agccagctta gattggtagt attccagaac ataccagggc atgagttgtt
    11461 ttagtttgtc gattcgttcc agtaaaatct cacgtttcat gtattttctc cataagtaag
    11521 tatactagta gtatagcata gacttgtata tttttcaaga aaattatagt ttttcagaaa
    11581 gatgttatag gagtttttca aagtgataaa aagacttaga cctacaaaca aaacagaata
    11641 aggaaaataa tgaaaatctc acccactaaa cttctgttaa tactacataa ctgaagagct
    11701 atataaggag ctatgagata caaaacacat tgtataatag attgaaacta gaatagtaca
    11761 tctggacttc taaaacattg ctagaaattg atttgattgt cctgattgtt ttatcctatt
    11821 tctatttcat ttcactatat aattgctatt gttttctttt tcatgatttt aatcttactg
    11881 tacagatttg atacagtaaa ctcctttcta gctttatgct ttatcatttc taacttaatt
    11941 atagtcttat ttttaataaa gttcaattat ttatatgtaa aattcctcat tagtaaaaaa
    12001 taaccgatct catttctgag aatcggtttt ctaaataaat caaactgatc atttacataa
    12061 tttatgttat gtaaatgatt atactacaac aacaaatctt tgacttttcc aatttcactt
    12121 tttggaataa ccaggtgaat catatcaccc agatacattc tggttgagcc gttaactgtt
    12181 tggctcttgc cattatggac ttgagttgtg atgaggacgt tgtgtggtaa gttgagttca
    12241 tgaacttgtt tcccagcaat tttatcagaa actggtattt cgataagtgt aacttctcct
    12301 tcgctagata cttcttctgg aagcattttt tccagcatgg cttcatagac tggcgtacct
    12361 ttgagcaaat ccatgataat ataagaaaca agagtgacaa gaccaagtgg cataaggttg
    12421 cgaatatctc ctaccatctc agttacgagg atcatagcgg ttaagggagc ttttgatatg
    12481 gctccaaaat agccactcat tcctagaatg acaaatatag ggaattgctc ttgactgaca
    12541 agtccaagat tgacacagat agcaccaatt aaggcaccaa gcaaagaacc aagagctaaa
    12601 atggggagga aaattcctcc tggcagtcca cttccatagc taatcatact ccaaataaag
    12661 cggatcaaaa agtaagctaa taaaacttgg aaactaaaat tttgttcagt taaagaaagg
    12721 acaagctgat ttccgccacc aatgatttga ggtaagaaga ttccgactgg tatgataagg
    12781 ataaaagcca agatgggata ataagctcta tccaaatgga ttttttgacc aatcaagtca
    12841 taaactcttc caacgtttaa tacagctttc tcatagagaa agcctgaaaa tccaaggaaa
    12901 attcccatgg cgagatatat ccaatactga tctagggtca tgggaggaat gttatctggc
    12961 atatccaata ctggtgtcaa accgaacatg agtagagaca caaagtttgc tacgatgctg
    13021 gctgctagag ttgagaccca gaaaaagcgc gaaaaatggt gatagacttc ttctacaaca
    13081 aagagaagtg ctgcaatagg agcattaaag gctgcggcta aacctgctgc agctccactg
    13141 gcaatcaaag agcgttcctc tactggactg gatttgagcc acttggcaat tcctttacca
    13201 ccaactgctc caagttgaat actgggtccc tctcgaccca gcatgagtcc actggcaata
    13261 gcaagaatac ctagcacata ttttttccaa agaatgcccc accagttgag ggacatgagg
    13321 cctttcagtt cggcttcgac ttgaggaatt cctgagcctt taatatcttt ttctgaccgt
    13381 gttagtttgg cactgagcca acagatgagt atataaaaca aaaccagtac aaaaagattg
    13441 cgcactaagt acccttgatc ttgataaact ccttgtatca ggtggaagcc cttttcaatt
    13501 aagaaacgaa aggatccgac gataattcca acgatgaggc cgacaatgat tcctcgacca
    13561 acttgggata gtatagtgct ggatgcaaag gcgaattctt tcttagaacg gactatttca
    13621 gactgttcct ccatggtatt tcctttcaat taactttctt ttttacctaa aaccagtgat
    13681 ttaacgggct caaagctggt tcggtgaatt ggggtaactc ctagttttgt gaggccttcc
    13741 agatgtttag ctgtgccata tcctgcatta gtagcgaaat catagccagg gaactgctga
    13801 tcgtattcct tcatcaattc atcacgtgtt accttggcta ctatagatgc tgctgcgata
    13861 gagagggagt tggcatctcc tttgataatg gaggtttgtg aaatgggcaa gtccagtttc
    13921 atggcatcaa tcaaaaggtg ctctggttga gggctgagct gggagattgc ttcttgcatg
    13981 gctagtttgg ttgcttcata gatattgact tggtcgatga cctgattatc tatgatacca
    14041 attccaatcg acaaggcttg gtcttgaacg gcttggaaaa tctccagatg tttcttttta
    14101 ggaattttct tgctgtcgtt gagaccttta atcttacaat ttttaggtaa aataacggcc
    14161 gcagcgacta caggaccagc aagaggacca tgaccaacct catcaatacc tgcaattaag
    14221 gtcaatcctt gcttataaag ttctttttca taagaaagca tggattccaa gcgcaaattt
    14281 tcatctaatt cagcttggat ggctctttta cgcttgctga tttccttttg aactcctgag
    14341 cgattatcct tttcaagctc taaaaaaata gggctttcta actccttgac tgtcacaagg
    14401 agttctttga tttctttaat cgttgccatc gaggtcttcc aatgtatcta aggtatagtt
    14461 accgagtttg ccatcacgga cttccttcac gaagagactg taaaaacggt catagtcatc
    14521 acggaaaccg agggcgcggg tcatatccat aataatcaca ggcgcttctt cttcaatttt
    14581 catttgtttg aagcgttcag ccagcttttc tggataatgt tctttgaaat aattgatacc
    14641 aaaaatggta acctcatcca taggaagcaa ctgatccttg atagctccag tcaatgccaa
    14701 cttaagtgca acagtttcat cctcaaactt aggccagaga atccccggtg tatccaagat
    14761 ttccagatct ttattggttt taagccattg ttgacctttt gtgacccctg gcttgtttcc
    14821 aacaacagca atctttttac cagccaaacg gttcatcaga gttgatttac cagcgtttgg
    14881 aatcccaata atcatggtac gcaaggtttc aatctgaatc ccacgttctt tctggcgagc
    14941 aatcttatca gccatgagct tcttggccgc atctgttaca acttttacag tcacttgttc
    15001 tttggagttg atagctagcg tctggattcc ttgtgattca aaatactgac gccattcctt
    15061 ggtcattgct ggatcagcca agtcggcctt gtttaaaatc aagagttttg gtttatcacc
    15121 aacaatcttg atcaacatag gattttgact agatagaggt aagcgtgcat caactaaaat
    15181 cgtcacaaaa tcaacaaatt ttaaattctc ctgcacctgt cgacgagctt tagacatgtg
    15241 accaggaaac cattgaatag tagccattga gttttttcct ttcgtagcaa ggatttagat
    15301 cccctatttt attttactat agtttgaaca tagagagtct tcaaatggat gatgtccaaa
    15361 cagttcttgt tttatgacat agaaatctac tttatattat atcatgcctt ctttattttg
    15421 tccgatgata gccttttacc ctatatagaa aaaagccatc aaatgatgac ttttcagagg
    15481 gagcaaaaaa ctgttcccgt catattatga accaaaagaa ctaaactacc aacagctaat
    15541 aggcctaaag ctgtaagcgc agaagttgat gaacctgttg caggaagagt ttcctgctga
    15601 taaataagag cataacttac ttcattatca gtaggaattc ctgcttgaat cttggtttgt
    15661 tcttcatcac ctttttaaac tttatttcct aatagtaaac actatttttt attctcaaaa
    15721 attgaacttc taaaatcatt tgtttggtca agataggctt taaagattgc tctcttcaac
    15781 ttatgaactg cactgtctat ttctggatta tagttatacc aatgaggagt ttctgtatcc
    15841 ttgcggacag cttcattcat taagttctgc agttcattta cactgtgaat tgtcttaata
    15901 atattgcgcg tccaaggttg agacggatct ttaaatgaaa ttttgttcaa tttattgaat
    15961 ttatcctgac gttctttata catagctttc ttaaaatcac tccaggtatt gtattgtcca
    16021 ttaaataatt tttgaagaac taatgtatct gttactaatc ctttggtctt accatatgag
    16081 gtaatcgttt tattatttgc cctagcatct gcttcaaatt gatttgagat atatggcacc
    16141 ataccatctt taaagccttt tgcagctaat agttcatagg ccatacgacg tcccatcaga
    16201 tcacctggcg ttccgtcatc attacttaat gctgcataaa tcggcgcaaa taatttaata
    16261 gtgaaatagc catttctttc gtatttacct gaggcatatt ccctagacga aagaatatta
    16321 ttatcaatca aatcattgaa tgaacgtagt ctttcaactt cttctactgt tatatttctg
    16381 acaacatttg ttgcatagac cttatttcca tcaggatcag gatggtactc atttgtaact
    16441 tttctaagaa gttgttgttt ttgattcgta tccaatttaa gaattgaatt tccttcgaga
    16501 tattccaaca tataaacaac gtcaaacatg ttgtggacat attgcttcaa atcatctgca
    16561 ttattaaatc ttgtagttgg atcaagtact tgtaatcgtc gactttctgt actatcagat
    16621 ttcgaatgtt tcaagatgga gttgatggta atggtcgcat catctggatg gtctggtgct
    16681 tgtaataatc ctttagcaaa gaactctggt cccaagccac ttcttcgacc atatcctcca
    16741 agataaatgt cctgatctga gtcatgtgtc atctcatgcg tataagtaat agctccatcc
    16801 ttatccaaca ttcgataacc catataataa actgcatcac ctgtagcata agcaccgtgt
    16861 tgattatgcc caactttatt tccaacaggt ccaaagaaat gttgcattgc aggatttgga
    16921 ttatcaaaat ctgccacttc tgtagctttc cctacggtat tatcatcgcc aaatttataa
    16981 gcatcgtaaa gcaaaatatt tctataaagt ttttcacgtg cattgtcgtc taaaatacga
    17041 taccaataat cgtagtgatc tcgctgacgt ttggctgttt cacgtgcatt ttcttcaaca
    17101 aaatcattga ggtccttgcc tgctttgtgc tcactattgc ggtaacgatc ataagctcca
    17161 aatcctagac tagagatggt cgagatgaca aagactgatt tctccggcaa ggttaggagc
    17221 ggcaagacca tattgcggta tttccatgtg gcactagtga tacggtcata gacaccaata
    17281 gaatacttag tgccaactag tccttgcttc gttttcacct cttcgatatt ggatttttct
    17341 tcgacaatgt aagccttagt ctgtgattta aaccattcat tgttagtttg atgcggtgca
    17401 aatgctttac ggtagccttc taaagcacta aacagatctt ttgtaccgta gttattcgtt
    17461 aacaaggcat tgtaagtaac cacattattt ttggccaaga gattgttgta gccagattta
    17521 cctaattcga taattgcatc tagcggagag gtatttccct taccaaagaa atccatatgg
    17581 aacataagca aatcttttgc gctagcctta tcgaatttga aatcatacca gcgttctaga
    17641 taggttaatc ctagcatcaa ggcttctttg tttcgcttga ttttatcaac aatgtaattg
    17701 tcaattatat cattatttcc agaaacagca gcatcagctg aaagtaattt tttaagagtt
    17761 tcagttaagt gctctttggt cttagtgaat tgctcatcta gataaagttc tgttaattta
    17821 acattatcag agattcctaa caaattctta agagcagttg gattatacgg tactgcagtt
    17881 aaatcatttt taactctgtc tattataggc gtataatttt ttaggaaagc atttggtgtg
    17941 tatactagac cagtattttc aatattatac tcagccagat ttgcaaagtc actattgtag
    18001 gttaaatcta ctttctcaga tgttccgtct ttgtaatgta gtagtaagtg attgatagag
    18061 ttcttgtttg aagtgatatc tgagactact tcatcatttt tcatagctac aacagacaac
    18121 aattctttct taaacaggtt atgatcagta gataattgat ttccatattt cacaatagta
    18181 tctctattat agaaaggtaa taatttttct atatttttat atgcaacttc tcttgttgat
    18241 tgataatgtg ctgccttggt ataatctaca tcctgtttaa cagaatttgt gaaatcttcc
    18301 actgtgatag tgtgtttttt agtaatctca tctttcttag cttgaactga tgttttttca
    18361 atctcagtac cacgttctac agtggaatca actgacatta cctcatctcc aactgcttga
    18421 acattgacta ctttgttttc tttattacta tagacatttg tttcctttat accattgaag
    18481 tggtaaccag aaatcgcatt cccatttgta atattcatat cgctgagaac atttgtcaag
    18541 cgaccagtat tgttatctgt tccgccttct tgtgtccata gggaacctgc aactccacca
    18601 actctagcga atggtttagc attatttatt tcaccttcag cataactatt ttctacagtc
    18661 gcattatttt ctacaatacc aacaatacca ccaattgttt gattagaatg ttctccatta
    18721 cttgaaatga ctactgttga aatagaatga gctaccctag cattagcacc ggttaattga
    18781 ccgactagac caccaattgc atactgagaa gttgtaacaa aagtatttgt tattcttccg
    18841 gtgaagctac tatttcttac tgtagaatta tcaacttttt ggacaagtcc tccgatacct
    18901 agttcaccag caatgattcc atttgcgtga acgttatcta aagttgaaga atttgtggct
    18961 tccatagcaa ctgatgcgac atgattctta ccagaaatat tgacatcttt caaggtgaga
    19021 tcctgaatag cagcattatt taatgtattg aataatggct ttttcaaatt atagatagca
    19081 taatgtttac cttgatgact accaattaat gtccctgtga aattctttgt aacataactt
    19141 tcttgatttt cacccaactc aaactcacga gcattcatgg tcgcgccaag tataaatgtt
    19201 ccatcaggtc tactattcat atcagttact aattttttaa atgatgtgta gatagggcca
    19261 tcgttttgaa caacttttgg aagataataa gtaaaattat cttggtattg attgtctaca
    19321 gattgtttta agtgttccaa tctagctgta attttataga ctactttacc gtcttttgta
    19381 gtgtcctcaa tgtttgaaac tgggagcagt acatctttaa atttagctga cttcactttg
    19441 atgaaataag cagatttatt atctggtact ttatctaaga ttgtatgttg ttttgattga
    19501 ttagcttcat aactatagag ttccacatca gaaacacttc ttagttctat tgttttctct
    19561 ggttcttcct cttgaagttt ctcaaccgga gtttcaggtt ttacttgctc aatattcccc
    19621 ttatattctg gcaatggtgc tacctgctct ggttcacctt tatcacttac tactgcttcg
    19681 ggtaattcag gctgaacttc tggtacgcct ttatcactta ctacagcttc gggcaactca
    19741 ggctgaactt ctggtacgcc tttatcgctt acaactgctt cgggtaacgc tggctggact
    19801 gcgggttcaa caatagctcc ggcttgtacc cctgtgtatt ctggtagtgg tgctacctgc
    19861 tctggttcaa caatagcgct ggcttgtggt cctgtatatt ctggaagcgg tgctacctgt
    19921 ttaggttcac ctttatcact taccacagta tctggcgact ctggttgaac ctcagtctca
    19981 cctttgtcag tcacaaccgc ttccggtagc tctggttgga ctgctggttc acctttatca
    20041 cttactacag cttcgggcaa ctcaggctga attgcgggtt caacaatagc tccagactgt
    20101 acgtccttat gttctacacc agtctcaggt tgttccttta taacttgagt ttttttagta
    20161 cctttttcga ctattcttgg actaggcgca gtcgttgaag ttgaaacaat ttctcgcgaa
    20221 acttcttcct tgtttacaga gaatattctg acgatttcaa ctttcttacc taatttacct
    20281 tcttgtttta ctcttacagt tccttcagct aaatcaggat tttcttgaat ttcttcttga
    20341 aaatctattt ttgtctctat agtttcctca cgatataaga gttcaggttt gttcaattga
    20401 cctgataaaa cttcatcctg tggatttaat gtatttaccc cagtcttttc ttttggagaa
    20461 atcttctcct ctttcttcgt ttctagattc ttatgttcgg ctaattgttc ttgagaatct
    20521 gaagattgtt tctcttcttt tcttggattg attaattcag tagagaaagg tttttcaact
    20581 acttgaactt ctgtcggctt agttgaagaa acaggtgttt gttcctgaat agcttgtact
    20641 gttgatggat ggtctacaaa atttggtgta acattataat ccaccttttg ttgttttgta
    20701 ggagtggctg ctgatttttc ttgattactt acttcaaaat cagaagtcgt ttttccctct
    20761 ttgatatatc caatataagt gtaacctgaa atctctttag gaagaggtaa tttttctcca
    20821 gaggtcaatt catagtccgt attgtaattt agcaaaagat gattttctaa agcatggact
    20881 gaaactaaga caccatttcc cattcccgca accaatacta aatgtaatac cgttttattc
    20941 ttaacctttt tcttggaaac agcaaaaatt aaaattccca gagcagctaa gctagcacca
    21001 gcaactaggg cttgcctctc attcttgctt ccagtatttg gtaattccgc cagttgattt
    21061 tgagaattta acttataaac aagataataa gtttcatcat cattctccac gtatgtcgga
    21121 atatcataga caagctgctt cttttcttct gatgatagct ctgaatctgc cacatattta
    21181 tagtgaactc ccgcagtttc ttgagcatcc acagatgaac tagctaatac agacataaaa
    21241 aataaacttg aaatcgttgc agatacaagt cctactgata attttctaaa tgaaaaacgc
    21301 tcttgttttt caccaaaata cttttccatt attcctcctt gaaataaaat ttatatatgt
    21361 tacaaagacc tttattatat tagtgtatta tctattatct atagaaaagg cagtatacct
    21421 taattatact cttaatttac aaaaaagtct taaaattgag atgcgctttc atactttgtt
    21481 ttatattatt ttggggtaca ataacaccta ccatgaaatt tacacggtag gtgttactca
    21541 tatcactaat cgtcctaaaa atggtttgag gcagttgagg agaattcctt ctatccagct
    21601 tccttgtgct gatgagcgat ggtcttcctg caggcttttt tttagaaaat ctcggacttg
    21661 ttctggtgcg atttcaaatt caaaggcttt cattttatag aaaaagtcga tgagatgatc
    21721 tgacaggtat tcagttgaaa agggtacttc accacttttt cgatattcta ataagagcct
    21781 agaaaatcga gctttttctt caggaagctc tcgaaaatag gaattgagga gccaagtctg
    21841 cttctgtttt ctttcaattg gatcctgact ggcaattcgt tggtcttttt ccagctcttt
    21901 ttggtattgt ttggccttga tagcccgttc tgctctattt ttaccaaaaa gaattttttc
    21961 ccacttgcgt tcttcttgag tcagggtctc tgtaaagcca aagtaatctt gataagcacg
    22021 ctctgcgggt cccatggcta gaaccagatt atctgcatat tgcttggcga ttttatccct
    22081 cttcttgcgt tctttctctg cctggatacg gagttcttgt tcgtagtcaa ttttctcctt
    22141 gcctagcttg acaaggtaga gttggtcatc cgatttccca agtaaaaatg gtttgataca
    22201 cttttcaagg acttcttcca tccgagcctt tttctttggt tccgccttgg tccaacttcc
    22261 tccctgaaag acttctagga aaagctggta gtctctctca ggcgcaaatt gattgccacg
    22321 attgggtttg aaaacacctt tttcccagag ccattttaga agtcgctcgt caaagtcact
    22381 tttattgacc ttgatttttt cctttttctg agcttttctg gttagatttt caacctttct
    22441 gagcagtttt tcttcctctt ccaattgctg gtcaagggac aatcgatgaa aatgacgaac
    22501 acagtcgcta ccaattggaa agaggcgttg gcctgtgaca ccgttaaaga gttcataagc
    22561 gtatttgatg gcatttccac agacacaatt gctacggccg ataccgttaa acatcaagga
    22621 aacttcattc cattccttgg tagcttgttc ccaagtatcc gctttcgaag cctgtaaaac
    22681 tgcatcgtgc agggattttc taactggaag tgtcatgagg tctcctttct aatactcaat
    22741 aaaaatcaaa gagcaaacta gaaagctagc cgcagtcagc tcaaaacact gttttgaggt
    22801 tgt
//
LOCUS       DAVKJJ010000035        20296 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000035 DAVKJJ010000000
VERSION     DAVKJJ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20296
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.035"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            38..382
                     /locus_tag="VW144_001366"
     CDS             38..382
                     /locus_tag="VW144_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600809.1"
                     /translation="MLDILLGTFLQISIVSIGWLVLYSGLFEAGVFLLANKGVAVKIK
                     EVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHIFIVFNALMYLLSS
                     SQVSLKKNKLSS"
     gene            434..1123
                     /locus_tag="VW144_001367"
     CDS             434..1123
                     /locus_tag="VW144_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9600810.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNFV
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVFQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            1165..1413
                     /locus_tag="VW144_001368"
     CDS             1165..1413
                     /locus_tag="VW144_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600811.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLKWFVLERLDLKNDV"
     gene            1443..2054
                     /locus_tag="VW144_001369"
     CDS             1443..2054
                     /locus_tag="VW144_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9600812.1"
                     /translation="MEFFDKFHALCFGFLVLIIVIIVPYTINHGDFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGLAYKKSQNLWVSTLVHMLYNSWPLLYYL"
     gene            2216..3010
                     /locus_tag="VW144_001370"
     CDS             2216..3010
                     /locus_tag="VW144_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEU9600813.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            3007..3642
                     /gene="trmB"
                     /locus_tag="VW144_001371"
     CDS             3007..3642
                     /gene="trmB"
                     /locus_tag="VW144_001371"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEU9600814.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEAGVSNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            3768..4247
                     /gene="rimP"
                     /locus_tag="VW144_001372"
     CDS             3768..4247
                     /gene="rimP"
                     /locus_tag="VW144_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU9600815.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEMISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            4291..5427
                     /gene="nusA"
                     /locus_tag="VW144_001373"
     CDS             4291..5427
                     /gene="nusA"
                     /locus_tag="VW144_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEU9600816.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            5449..5742
                     /locus_tag="VW144_001374"
     CDS             5449..5742
                     /locus_tag="VW144_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEU9600817.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            5735..6034
                     /locus_tag="VW144_001375"
     CDS             5735..6034
                     /locus_tag="VW144_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEU9600818.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            6051..8843
                     /gene="infB"
                     /locus_tag="VW144_001376"
     CDS             6051..8843
                     /gene="infB"
                     /locus_tag="VW144_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU9600819.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     AKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            9094..9444
                     /gene="rbfA"
                     /locus_tag="VW144_001377"
     CDS             9094..9444
                     /gene="rbfA"
                     /locus_tag="VW144_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU9600820.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            9723..10502
                     /locus_tag="VW144_001378"
     CDS             9723..10502
                     /locus_tag="VW144_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600821.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQGTTTLLSQEILDRVANACIRDNVLLASGTSVLAGLAGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWASREELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLRIFGVNLTKGGLAKGIGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIKGE"
     gene            10504..10686
                     /locus_tag="VW144_001379"
     CDS             10504..10686
                     /locus_tag="VW144_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600822.1"
                     /translation="MNPIKAFAKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(11041..11115)
                     /locus_tag="VW144_001380"
                     /pseudo
     CDS             complement(11041..11115)
                     /locus_tag="VW144_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            11146..11379
                     /locus_tag="VW144_001381"
     CDS             11146..11379
                     /locus_tag="VW144_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEU9600823.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            11379..12713
                     /locus_tag="VW144_001382"
     CDS             11379..12713
                     /locus_tag="VW144_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEU9600824.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYELMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKLLPEVSISSVKEVFEAFAIEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDVYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKAIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDENGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            12723..12977
                     /locus_tag="VW144_001383"
     CDS             12723..12977
                     /locus_tag="VW144_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEU9600825.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            13073..13468
                     /locus_tag="VW144_001384"
     CDS             13073..13468
                     /locus_tag="VW144_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU9600826.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGINSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            13838..14329
                     /locus_tag="VW144_001385"
     CDS             13838..14329
                     /locus_tag="VW144_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU9600827.1"
                     /translation="MTTCSSSCSLGDWVVIGVVARENAAEQIKQYQKFTVNISDETSM
                     LAMEQAGFISHQEKLERLGVHYEISERTQTPILDACPLVLDCRVDRIVEEDGICHIFA
                     KILERLVAPELLDEKGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKD
                     GKN"
     gene            14316..14876
                     /locus_tag="VW144_001386"
     CDS             14316..14876
                     /locus_tag="VW144_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9600828.1"
                     /translation="MARTELPDKIEIERLVLRVRTVADAEDIHVYASLPEVAYPAGFL
                     PVKTLEDEIYYLGHILPNRNQKENLPAGYGIVVKGTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSQRVAEKLGFTLEARI
                     RDRKDAQGNCCDDLRYALLKSEWGVI"
     gene            14876..15454
                     /locus_tag="VW144_001387"
     CDS             14876..15454
                     /locus_tag="VW144_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9600829.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYHLIYLKQIQNLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKHKPLKRHIEVSEREILETAETLQLNSQYQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            15447..18098
                     /locus_tag="VW144_001388"
     CDS             15447..18098
                     /locus_tag="VW144_001388"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU9600830.1"
                     /translation="MSKELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVRDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTDDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNEAGEIYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHSIMPFVTEEIFGQYAEGSIVTAAYPTVNPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ERDKQADYQAKYDATVARIDEMKKLVK"
     gene            18169..18924
                     /locus_tag="VW144_001389"
     CDS             18169..18924
                     /locus_tag="VW144_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEU9600831.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCSSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIIKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSLEDLY"
     gene            19434..>20296
                     /locus_tag="VW144_001390"
     CDS             19434..>20296
                     /locus_tag="VW144_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600832.1"
                     /translation="MERSRFEEMFQLQSSHLTTQEKSQLFTSVFAGRYDVYAKNFINE
                     QGKIQYFPSYDYGWKQLPPEKRSFQTLTNSVLKSHFRGEAAIGIFPMHLDDSCYFLVL
                     DLDEGDWKEAGLTIRRIARERQMEAHLEISRSGYGLHIWFFFEEAILSRKARLFGKKL
                     LELAMQESMQLSFDSFDRMFPNQDVLPKGGFDNLISLPFQGEAYHQGRTVFVDEQFQP
                     YEDQWRYLQEIQKISTAKVALLIQEELGKQELDKELKVVLSNMIQLKKSSVTPKTLFF
                     LKNMASFSNPE"
BASE COUNT         6108 a         3785 c         4610 g         5793 t
ORIGIN      
        1 gaaatacaga ttattttttg ttattttctt gagtagtgtg ttggatattc ttttagggac
       61 atttttacaa atctctatcg tatctattgg gtggcttgtt ctttacagtg gtttgtttga
      121 agcaggagtt ttccttcttg ctaataaagg ggtggcggta aaaatcaagg aagtagatat
      181 tcgaaatcgc tttaaattta tttttggaaa aaccttatgg tttcaaattc ttttgctcat
      241 ctttttgata atcaaacttt atcttggttt ggatgcgagg ttgattttat tctatggaca
      301 tattttcatt gtctttaatg ccttaatgta tctattatct agtagtcagg ttagccttaa
      361 aaaaaacaaa ctgtcttctt aatcttacag cagtagagcc tgacaaggtg aggtcatagt
      421 agggaggatt tctatgaaaa agtatcaact tctattcaaa ataagtgcag tcttctctta
      481 cttatttttc gtatttggtc tttctcagct gacgcttatc gtccaaaatt attggcaatt
      541 ttcttcccag attggtaatt tcgtctggat tcaaaatatc ttgagtttac tttttattgg
      601 agtcatgatt gtggttcttg ttaagacagg ccatggttat ctcttccgca ttccaagaaa
      661 aaaatggctt tggtattcga ttttgacagt attagtggta gtgttccaga tctcttttaa
      721 cgttcagaca gctaaacatg ttcagtcaac tgcggaaggt tgggctgtat tgattggtta
      781 tagtgggact aactttgcag agctaggtat ttatatagcc ctgttctttc tggttccact
      841 gatggaagag ttaatctata gaggattact gcaacatgct ttctttaagc attcgcgatt
      901 tggtcttgat ttgcttcttc cttctatttt atttgctctc cctcattttt caagcctgcc
      961 tagtctgtta gatatcttcg tctttgcaac agttggaatc atctttgctg gtttgacccg
     1021 ctataccaag agcatttatc catcctatgc ggtgcatgtg atcaataata ttgtagcgac
     1081 cttcccgttt ttgctcactt ttctacatag ggtcttgggg taaaaaaaca aaaggcttgc
     1141 ttttcagcca tagaggaggt catcatgtat aaacacttat ttttcctaga ttccaaaact
     1201 ttagatcggt tgacacccta tattctagtc ttggcttctg acaccattgc ttttaatgtt
     1261 tttgtgctaa cctttgtatc tgcggtggtt tttaatttcc taaattccat gctagcttta
     1321 atggctatat tcataggggc tggctatgtg gtcggatttt ggttgctaaa atggtttgtt
     1381 ttggaaaggc tagatctaaa gaatgacgtg taggaagtct gtttgttgag gaggataatt
     1441 ttatggagtt ttttgataaa tttcatgcct tgtgttttgg atttttagta ctaataattg
     1501 tcattatagt tccttatacg attaaccatg gggatttttt tcaaaatgaa tctgcattga
     1561 ttcttgtaag tcttcttgta acctcgctga gtgttgctta tgctagaaag tttgaaatga
     1621 tttcttttgg gatgttaagc aagaaacaac ttttgctttt cattgcaatc tttcttctaa
     1681 gtgtacttga gacgctggtt tatattcatt tcttcgctgt ttcttctggc tcaggggtcc
     1741 aacacttggc ggaagtcagc agaggaattt ccctgtcttt gattttgact acctcagttt
     1801 ttggccccat ccaggaggaa ctcattttca gaggacttct tcaaggtgcg gtttttgaca
     1861 attcttggtt agggcttgtg ctaacttcct ctctcttttc tttcatgcat ggaccttcta
     1921 atgtcccttc gtttattttt tatctacttg ggggcttgtt gctgggcctt gcttataaaa
     1981 agagccaaaa cttatgggtt tctactctag tccatatgct ttacaacagt tggccactct
     2041 tatattattt ataaaaatca tgaaaaggta agtaggagat tgtgcttacc tttttctttc
     2101 tataatgata acccaaacca ctccagaggc ttttctttga gaatcgggtt agagtggttg
     2161 acgaataggc caaaaactag tagaatagta aggaaacttt atacggagga aagaaatgga
     2221 tttgggtgat aatgagctaa cactgactcc catacctggg aaaagtggca aggcttatat
     2281 gggtagctat cctgatggga agcgcatctt tgtaaaaatg aacacctctc caatcctacc
     2341 tggtctagct agagaacaaa ttgctccaca attattatgg agtcgtcgtt tggcagatgg
     2401 gcgtgatatg tgtgctcaag aatggttgac aggcaagata ttgaccccct atgatatgaa
     2461 tcgtaagcaa atcgtcaata ttttaacccg tcttcatcgc tcacgtccgt tgatgacaca
     2521 attgagtcgt ttgggctatg ccatggaaac acctgtagat ttactacagt cttggcaaga
     2581 aacggctcca gatgctttgc gtaaaaatca ttttatcagt gaagtgatgg ctgatttacg
     2641 tcagactatt ccaggattta gagaggacca tgcgaccatt gtccatggag atgtacgaca
     2701 tagtaattgg attgagacag atagtggctt gatttattta gtagattggg attcggttcg
     2761 cttgaccgat cgcatgtttg atgtggccca tatgctctgc cattatattt cagaacatca
     2821 gtggaaggaa tggttgacct actacggtta caagtacaat caaacggtat taagtaaatt
     2881 gtattggtat ggtcaattgt cttatttgag tcagatttcc aagtattata tgaaccaaga
     2941 tttagaaaat gtcaatcggg agattcatgg tttgcgtcat ttccgagaca agtatggaaa
     3001 gagaagatga gagttagaaa tcgtaaaggg gcaacagaat tactagaggc aaatccccag
     3061 tatgtggtcc tcaatccctt ggaagccaag gcaaaatggc gggacttgtt tggcaatgat
     3121 aatcccattc atgtggaagt tggaagtgga aagggtgcct ttgtttcagg tatggccaag
     3181 caaaaccctg acatcaacta tatcgggatt gatattcaaa agtctgtttt gagctacgct
     3241 ttggacaagg tgcttgaagc tggagtgtct aacatcaagc tcttgtgggt agatggttct
     3301 gacttgactg actactttga agacggtgag attgatcgct tgtatctgaa cttttcagat
     3361 ccatggccga aaaaacgcca tgaaaagcgt cgtttgacct acaagacctt tttggatacc
     3421 ttcaaacgta tcttgcctga aaatggagaa attcatttca agacggataa ccgtggcttg
     3481 tttgagtaca gtttagtgag cttttctcaa tatggcatga aactcaatgg tgtctggcta
     3541 gatttgcatg ccagtgattt tgaaggcaat gtcatgacag aatacgagca aaaattctca
     3601 aacaaggggc aagttatcta ccgagttgag gcagaatttt aagagataac ctaaaattag
     3661 gctgtacaag tgcttttgct ttacataagt tggcaaacgt gctatactga tagtaagaat
     3721 atgaaaagtg aggcggggaa atatcttcgc ctcttgctta tgaggaggtg gacgcaatcg
     3781 caacaatcgt agaattagtc agagaagttg tagaacctgt catagaagct ccttttgaac
     3841 tcgtggatat cgagtatgga aagattggca gtgacatgat tctcagtatt tttgtagata
     3901 aacccgaagg aattaccttg aacgacacgg cagacttgac agaaatgatc agtcctgtcc
     3961 tagacaccat caagccagat cccttcccag aacaatattt tctagaaatt accagtccag
     4021 gtttggaacg tcctttgaaa accaaggatg ccgtcgctgg agcggttgga aaatacatcc
     4081 atgtcgggct ctaccaagcc atcgataagc aaaaggtctt tgaaggaacc ttgttggcct
     4141 tcgaagagga cgagttgact atggaatata tggacaagac gcgtaagaaa accgtccaaa
     4201 ttccatacag tttagtatca aaagcacgtt tagcagttaa attatagaaa aagaaaggat
     4261 agcttttgag gattcaaaag tgaagaaaac atgagtaaag aaatgctaga ggccttccgc
     4321 attttggaag aagacaaggg aatcaaaaaa gaagatatca tcgacgcagt agtagagtcg
     4381 cttcgttccg cttatcgcag acgctatggt cagtcagaca gcgtagctat tgacttcaac
     4441 gaaaaaacag gtgactttac agtttatact gtccgtgaag ttgttgatga agtatttgat
     4501 agccgtttgg aaatcagctt gaaagatgct cttgccatta attcagctta tgaacttgga
     4561 gacaaaatca agtttgaaga agcaccagct gagtttggtc gtgtagcagc ccaatctgcc
     4621 aaacaaacca tcatggaaaa aatgcgcaag caaacacgtg ccatcactta caatacttac
     4681 aaagaacatg agcaagaaat catgtctggt acagtagaac gctttgacaa tcgctttatc
     4741 tatgtcaacc ttggtagcat cgaagcccaa ttgtcaaaac aagaccaaat tcctggagaa
     4801 gtttttgctt ctcatgatcg tatcgaagtt tatgtttaca aggttgaaga caaccctcgt
     4861 ggtgtgaacg tctttgttag ccgtagccat ccagaaatga tcaaacgttt gatggagcaa
     4921 gaaattccag aagtttatga tggaactgtt gaaatcatga gcgtggctcg tgaagcaggt
     4981 gaccgtacga aggttgctgt tcgtagccac aatccaaacg tagatgctat cggtacaatc
     5041 gttggacgtg gtggtgctaa tatcaagaag attactagca aattccaccc agctcgttac
     5101 gatgctaaaa atgaccgcat ggtaccaatc gaagaaaata tcgatgttat cgagtgggta
     5161 gcagatccag ctgaatttat ctacaatgcc atcgctcctg ctgaggttga ccaagttatc
     5221 tttgatgaaa acgacagcaa acgtgccttg gtggttgttc cagataacaa gctttctctt
     5281 gccattggtc gtcgtggaca aaacgtgcgc ttggcggctc acttgactgg ttatcgtatc
     5341 gatatcaagt ctgctagcga atttgaagcc atggaagacg ctgcttcagt agagttggaa
     5401 gtagaaaacg atactgtaga agaataaaag ctgctagagg agggaaagat gaaaacaaga
     5461 aaaatccctt tgcgcaagtc tgttgtgtct aacgaagtga ttgataagcg tgatttgctc
     5521 cgcattgtca agaacaagga aggacaagtc tttattgatc ctacgggcaa ggccaatggc
     5581 cgcggcgctt atatcaaact agacaatgca gaagccctag aggcgaaaaa gaagaaggtc
     5641 tttaaccgca gctttagcat ggaagtggaa gaaagctttt atgacgagtt gatcgcttat
     5701 gtggatcaca aagtgaaaag aagagagttg ggacttgaat aagcaaaaga taagtaatct
     5761 cttggggctt gctcagcgag cagggcgcat catatcgggt gaagaattgg tggtcaaggc
     5821 cattcaagac ggcaaggcca agttggtctt tctagctcat gatgctggac ccaatctgac
     5881 caagaagatt caagataaaa gtcattatta tcaagtagaa attgtaaccg tgttttcaac
     5941 actggaatta agcatagcag tcgggaaatc gagaaaggtt ttggctgtaa cagatgctgg
     6001 atttacaaag aaaatgaggt ctcttatgga atagaagagg aggacatgat ttgtctaaga
     6061 aaagattgta cgaaatcgca aaagaacttg gaaaagaaag taaagaagtt gtagcgcgtg
     6121 caaaagagtt gggcttggat gtgaaaagcc actcatcaag tgtggaagaa gctgtcgctg
     6181 caaaaattgc tgccagcttt aagcctgcag ctgctccgaa agtagaagca aaacctgcag
     6241 caccaaaagt aagtgcagaa aagaaagccg aaaaatctga gccagctaaa ccagctgtag
     6301 ctaaggaaga ggcaaaaccg gctgagccag ttgctccgaa aacagaaaaa gtagcagcga
     6361 aaccgcaaag tcgtaatttc aaggctgagc gtgaagcccg tgctaaagag caggcagaac
     6421 gacgcaagca aaataagggc aataaccgtg accaacaaca aaacggaaac cgtcagaaaa
     6481 acgacggccg taatggtgga aaacaaggtc aaagcaaccg cgacaatcgt cgctttaatg
     6541 accaagctaa gaagcagcaa ggtcagcaaa aacgtagaaa tgagcgccgt cagcaagagg
     6601 ataaacgttc aaatcaagcg gctccacgta ttgactttaa agcccgtgca gcagccctaa
     6661 aagcagagca aaatgcagag tacgctcgtt caagtgagga acgcttcaag cagtatcagg
     6721 ctgctaaaga agccttggct caagctaaca aacgcaagga accagaggaa atctttgaag
     6781 aagcggctaa gttagctgaa caagcacagc aagttcaagc agtggttgaa gtcgtccctg
     6841 agaaaaaaga acctgcagtg gatacacgtc gtaaaaaaca agctcgacca gacaaaaatc
     6901 gtgacgatta tgatcatgaa gaagatggtc ctagaaaaca acaaaagaat cgaagtagtc
     6961 aaaatcaagt gagaaatcaa aagaatagta actggaataa caacaaaaag aacaaaaaag
     7021 gcaataacaa gaacaaccgt aatcagactc caaaacctgt tacggagcgt aaattccatg
     7081 aattgccaac agaatttgaa tatacagatg gtatgaccgt tgcggaaatc gcaaaacgta
     7141 tcaaacgtga accagctgaa atcgttaaga aacttttcat gatgggtgtc atggccacac
     7201 aaaaccaatc cttggatggg gaaacaattg aactcctcat ggtggattac ggtatcgaag
     7261 ccaaacaaaa ggttgaagtg gataatgctg acatcgaacg tttctttgtc gaagatggtt
     7321 atctcaatga agatgaattg gttgagcgtc caccagttgt tactatcatg ggacacgttg
     7381 accacggtaa aacaaccctt ttggatactc ttcgtaactc acgtgttgcg acaggtgaag
     7441 caggtggtat tactcagcat atcggtgcct accaaatcgt ggaaaatggt aagaagatta
     7501 ccttccttga tacaccagga cacgcggcct ttacatcaat gcgtgcgcgt ggtgcttctg
     7561 ttaccgatat tacgatcttg gtcgtagcgg cagatgacgg ggttatgcct cagactatcg
     7621 aagccattaa ccactcaaaa gcggccaacg ttccaatcat cgtagccatt aacaagattg
     7681 ataaaccagg tgctaaccca gaacgcgtta tcggtgaatt ggcagagcat ggagttatgt
     7741 caaccgcttg gggtggagat tctgaatttg ttgaaatctc agctaaattc aaccaaaata
     7801 tcgaagaatt gttggaaaca gtccttcttg tagctgaaat ccaagaactc aaggcagacc
     7861 caacagtgcg tgcaatcggt acggttatcg aagcgcgctt ggataaagga aaaggtgcgg
     7921 tcgcaaccct tcttgtacaa caaggtacct tgaatgttca agacccaatc gttgtcggaa
     7981 ataccttcgg tcgtgtccgt gctatgacca acgaccttgg tcgtcgtgtt aaagttgctg
     8041 gaccatcaac accagtctct atcacaggtt tgaacgaagc accgatggcg ggtgaccact
     8101 ttgccgttta cgaggatgaa aaatctgcgc gtgcagcagg tgaagagcgt gccaaacgtg
     8161 ccctaatgaa acaacgtcaa gctacccaac gtgttagcct tgaaaacctc tttgataccc
     8221 ttaaagctgg ggaactcaaa tctgttaatg ttatcatcaa ggccgatgta caaggttctg
     8281 ttgaagccct ttctgcctca cttcaaaaga ttgacgtgga aggtgtcaaa gtgactatcg
     8341 tccactcagc ggtcggtgct atcaacgaat cagatgtgac ccttgccgaa gcttcaaatg
     8401 cctttatcgt tggtttcaac gtacgcccta caccacaagc tcgtcaacaa gcagaagctg
     8461 acgatgtgga aatccgtctt cacagcatta tctacaaggt tatcgaagag atggaagaag
     8521 ctatgaaagg gatgcttgat ccagaatttg aagaaaaagt tattggtgaa gcggttatcc
     8581 gtgaaacctt caaggtgtct aaagtgggaa ctatcggtgg atttatggtt atcaacggta
     8641 aggttgcccg tgactctaaa gtccgtgtta tccgtgatgg tgtcgttatc tatgatggtg
     8701 aactcgcaag cttgaaacac tataaagacg acgttaagga agttacaaac ggccgtgaag
     8761 gtggattgat gatcgacggc tacaatgata ttaagatgga tgatgtgatt gaggcgtatg
     8821 tcatggaaga aatcaagaga taagattttt tgctcctttc ttaggtggtg agggacgcaa
     8881 gcaaaccgat ggtttcattg cttatttttg agtctagggt ctcaaaaatc ccctgtgatg
     8941 ggactgataa atcagttcca tcactttcac cacggcgaaa gaagcagatg acttcaaatt
     9001 gaacttcgtt tcaatttaaa ctgaaaatca agaagtttaa aatagctagg tctgctggcc
     9061 tagcttttgg ttcaaagtag agaaaggaat atcatggcaa atcatttccg tacagatcgt
     9121 gtgggcatgg aaatcaagcg tgaagtcaat gagattttgc aaaagaaagt ccgtgatcca
     9181 cgtgtccaag gtgtgaccat cacagatgtt cagatgctgg gtgacttgtc tgttgccaag
     9241 gtttattaca ccattttgag taaccttgct tcggataacc aaaaagccca aatcgggctt
     9301 gaaaaagcaa ctggtaccat caaacgtgaa cttggtcgca atttgaaatt gtacaaaatc
     9361 ccagatttga ccttcgtcaa agacgagtcc atcgagtatg gaaacaagat tgacgagatg
     9421 ctacgcaatc tggataagaa ctaaagaaga ggggttgccc ctcttttttg gtggaggaaa
     9481 ataggttgaa tttgaaatgg aaaaatattc ttttaaaata tattgaaact agaatagtac
     9541 gcctctactt ctaaaacatt gttagaaatc gatttgactg tcctgatcga tttgtcctgt
     9601 tcttgtttca ttttactata aaaaaggggt tctgtatttt ttaatgttat ctaattagaa
     9661 aatgcttttt ttgtaggaaa tataatatga taaggtgtaa aaaagaaata aggagtttgt
     9721 atatggctga acaagactta gctatgcaag tattgcaaca agtggtgaaa ctacctgttg
     9781 ttaaggttga tcgttcgaaa tttttagtgg ataagttttc caaagaattg gatccaaaag
     9841 atattcctac cttattggaa caaggtacaa cgactcttct atctcaagaa atattagatc
     9901 gtgtagctaa tgcttgtatt cgggacaatg tattattagc gagtgggact tctgttttgg
     9961 caggattagc tggagggctt gctatggcaa ttaccattcc agctgatgtg gctcaatttt
    10021 atgctttctc tctgaaattg gctcaagaat taggttatat ttatggttat gaggatcttt
    10081 gggcttcacg cgaggagttg agtgaagatg ctcaaaatac cctcttgctt tatctaggcg
    10141 taatgttagg ggtgaatgga accgctgctt tgctacgtgc tggtggtata acaattgcca
    10201 aacaggtaat gaaaacagtg cctaataaag ctttaacaaa gacgctttgg taccctattt
    10261 tgaaaaaagt cttaagaata tttggtgtga atcttaccaa gggagggctg gccaaaggaa
    10321 tagggaaatt tattcctatc ttgggtggta tcatttcagg tggtttaacc tttgcaacta
    10381 tgaaaccaat gggggaaagc ttgcagaaag aattatccaa gctagtcaac tatagtgaag
    10441 ttcaatatca agaagatgtt gaaacaatcc gaaaagaggc tgaaatcatc aaaggagagt
    10501 aatatgaatc ctatcaaagc ttttgctaaa atttatggta attacttttt gaccgtgcaa
    10561 ggtgtaaaag tgatgaaaac gataaagaaa gctgaccatg tcgttgttgg tctgggcaaa
    10621 ctttttattg cagataagct aatggatacg gctcggtggc tcattaagcc agaggagaga
    10681 gaatgaaatt tttttggtct tcttgctatt ctttttatca aaccgattat tgggattgtg
    10741 aaattctttt ggatgatcat ttcttttgca gttcaattgc tgttttacaa gatattattt
    10801 aagatattgg attggctctt taaacttatc tagatggtaa tccaagtcgc agagagctag
    10861 caggaactcc actgctagtt ttttaatctc tctccatatg gtataatata agcagtaaaa
    10921 tcattttata ctcttcgaaa atctcttcaa accacgtcag cttcaccttg cagtatatat
    10981 gttactgact tcgtcagttc tatccacaac ctcaaaacgg tgttttgagc tgacttcgtc
    11041 agttctatct acaacctcaa aacactgttt tgagcaacct gcggctagct tcctagtttg
    11101 ctctttgatt ttcattgagt attagaacat ataatggagg tcgtcatgga caatatcatc
    11161 gatgtgtcaa ttcctgttgc agaagtggtg gacaagcatc cagaagtctt ggaaattcta
    11221 gtggagctgg gttttaaacc ccttgccaat cccttaatgc gcaatacagt tggtcgtaaa
    11281 gtatcactta aacagggttc taagctagca ggaactccta tggacaagat tgtacgcaca
    11341 ctggaagcga atggctacga agtgattgga ttagactaat ggcagatgaa cggattcatg
    11401 tcctacggga tattttatta gaattgcata atggcgcctc tcctgagtcg gttcaggagc
    11461 gttttgatgc gacctttgcg ggtgtctcag ccattgagat ttcccttatg gagcacgagc
    11521 tgatgaactc agactcaggt gtcacctttg aagatgttat ggaactctgt gatgtccatg
    11581 ccaatctttt taaaaatgct atcaaaggtg tcgaagtttc agatactgag catccaggtc
    11641 acccagttcg tgtctttaag gaagaaaatt tggccctccg tgcagctttg attcgcattc
    11701 gtagattgtt agatacctat gagttgatgg aagacgagga aatgcttgcg gagatgcgca
    11761 agggcttggt gcgtcagatg ggacttgtgg ggcaatttga catccattac caacgtaaag
    11821 aagaactctt ctttcctatc atggagcgct atggacacga ttcacctccc aaagttatgt
    11881 ggggagtgga tgatcagatt agggaactct ttcaaacagc tctaacgaca gccaagttac
    11941 taccagaagt gtcaattagc agtgtaaagg aagtttttga agcttttgcg atagagtttg
    12001 aaagtatgat tttcaaggaa gagtccatcc tcctcatgat tctccttgag tcttttactc
    12061 aggatgactg gcttcagatt gcggaggaga gcgatgtcta tggctatgcc atcatccgtc
    12121 cgtcagagaa atgggtgcca gaacgacaga gctttattga ggaaaagatt gcagaggagc
    12181 ctgtacagct agatacggca gaaggtcaag ttcaacaagt catagatacg ccagaaggcc
    12241 attttaccat tacctttacc cctaaggaaa aggaagctgt gctggaccgc catagtcaac
    12301 aggcttttgg taatggctat ctttcagtcg agcaggccaa tctcatcctc aatcatctcc
    12361 ctatggagat tacctttgtc aataaagaag atattttcca gtattacaat gacaatacgc
    12421 cagctgatga gatgattttc aaacggacgc cgtcccaagt cgggcgcaat gtcgaactct
    12481 gtcatccgcc taagtatttg gacaaggtca aggctatcat gaagggactt cgtgagggga
    12541 ccaaggacaa gtatgaaatg tggttcaagt ctgagtcgcg aggcaagttt gtccacatca
    12601 cctatgctgc agtacacgat gaaaacggag aattccaagg tgtactggaa tacgttcagg
    12661 atatccagcc ctaccgtgag attgatacgg actattttcg tggattagaa taaggagaaa
    12721 aaatgagtta cgaacaagaa tttatgaagg aatttgaagc ttgggtcaat acccaaatca
    12781 tgattaacga catggcgcac aaggaaagcc aaaaagttta cgaagaagac caggacgagc
    12841 gtgccaaaga tgccatgatt cgctacgaga gtcgcttgga tgcttatcag ttcttgcttg
    12901 gtaagtttga aaacttcaaa gtaggcaagg gattccatga tttgccagaa ggcttgtttg
    12961 gtgagcgaaa ttattaaacg agaaagattc ttgatttttc actaaaatct tgatagaatg
    13021 tttatgttaa atccttgtca gagcagggat tttttattga aaggatttta tcatgtcaaa
    13081 gaaactcaat cgtaaaaaac aattacgaaa tggcctccgt cgcgcaggtg ccttttcaag
    13141 tactgtgact aaggttgtag atgagacaaa aaaagtcgtg aagcgtgcag aacagtcagc
    13201 aagcgcagct ggtaaggctg tttctaaaaa agttgaacaa gcagtagaag ctaccaaaga
    13261 gcaagctcaa aaagtagcta attctgtaga agattttgca gcaaacttgg gtggacttcc
    13321 acttgatcgt gccaagactt tctatgatga aggaatcaat tctgcttcag atttcaaaaa
    13381 ctggactgaa aaagaactcc ttgctttgaa aggaatcggc ccagctacca ttaagaaatt
    13441 gaaagaaaat ggcatcaagt tcaagtaatt tttcttgagc cttgcatttc cgagaaaaac
    13501 ttgctacaat agagccatta gaggtgtttt gaatcccaca ttttacagaa agtggcggcg
    13561 ctgagaagtc cacaaatgtg tcaaaactgg ttgctaatga ctgaaaaaat gaaatatttc
    13621 tgtcttttta ttttaaagac taaagatgcg ggcttggccc gaaattgggt ggtaccgcgg
    13681 ataaacacat tcgtccctgt catgtagatg gcagggcttt tcttttacgt cttagtcaaa
    13741 gggggttgtt atgaagcaat cttttaacac aagtaaacta tactatggtt ttcctgtttt
    13801 tattttagga tatcaagatt agaattttgg ctataacatc acgacatgta gttcctcttg
    13861 tagtctagga gattgggttg tgattggagt cgttgcgaga gagaatgccg cagagcagat
    13921 caaacagtat caaaaattta ctgtgaatat ttctgatgaa acttctatgc ttgcgatgga
    13981 gcaggctggt tttatcagtc atcaggagaa attggaacgt ttgggagtgc attatgaaat
    14041 ttctgaacga actcagactc ctattttaga cgcctgtcca cttgttttag attgtcgggt
    14101 agataggatt gttgaggaag acggtatttg ccacatcttt gccaagattc ttgagcgact
    14161 tgttgcccca gaactcctgg atgaaaaggg acattttaaa aatcaactgt ttgccccaac
    14221 ctatttcatg ggagatggat atcagcgcgt ttatcgctat ctggacaagc gtgtagatat
    14281 gaagggcagt ttcatcaaaa aagcgaggaa gaaggatggc aagaactgag ctgccagata
    14341 aaatcgaaat agaacgtctc gttttacgag tccgcacagt ggcggatgct gaggatatcc
    14401 atgtctatgc tagtttgcca gaggtcgcct acccagcagg ttttctgccc gtcaagacct
    14461 tggaagatga gatttactat ctggggcata tccttcccaa tcgcaatcaa aaggaaaatc
    14521 tcccagcagg ctacgggatt gtcgtcaaag gaacggataa gatcgttggc tctgtcgatt
    14581 tcaaccatcg ccatgaagat gatgtgctgg aaatcggcta taccttacac ccagactatt
    14641 gggggcgagg ttatgtgcca gaagctgcgc gtgccttgat tgacttagcc tttaaagatt
    14701 tgggtcttca caagattgaa ctaacttgct ttggatataa ccttcaaagt caacgagtcg
    14761 cggaaaagct tggctttacc ctcgaagctc gcataagaga ccgtaaagat gctcaaggca
    14821 actgctgtga tgatttgaga tatgccttgc tgaagagtga gtggggggtg atttgatgca
    14881 tcttttcatc attggtgctc cagcatcagg aaaaatgacc attggtcaag aattatctcg
    14941 actgacggat gctaccctat tttataacca tcaagccatt gattttgcac tagaaatcta
    15001 tcaggactat acagaggaaa tgtgggaatt tgttcgtgga atcacttttt cattccttgg
    15061 agcaagtgca agaaatcagc gatcagtaat tttaacagat gtaattgatt tttcaaatca
    15121 gtatcatctg atatatttaa agcaaattca gaatttgttg gatgattatc atcaagagat
    15181 tctttttgtt gagttggaaa caagtcttga agagtgcata cgtcgaaatc gaacggagaa
    15241 tcgattaaag cacaaaccct taaaacgaca tattgaggta tctgaaagag aaattttaga
    15301 gaccgctgaa acacttcaat taaattccca atatcaaccg aatgagttgc accactactt
    15361 taaaataaat aatacgaatt tgtctgcaga agaagctgct aagcaaattc aaaataaaat
    15421 aaacaaaata gagaaaggac acacacatgt ctaaagaact ttcacctaaa tacaatccag
    15481 ccgaggttga ggctggtcgt taccaaaaat ggcttgatgc tgatgttttc aaaccttcag
    15541 gcgatcaaaa ggctaagcct tattcaatcg ttattccacc accaaacgtt acaggtaaac
    15601 ttcaccttgg tcacgcttgg gatacaactt tgcaagatat tatcatccgt caaaaacgca
    15661 tgcaaggttt tgataccctt tggcttcctg ggatggacca cgcagggatt gccactcagg
    15721 ctaaggtgga ggagcgcttg cgtggtgagg gcatttcccg ctatgacctt ggtcgtgagt
    15781 ctttcttgac gaaagtctgg gaatggaaag acgaatatgc cactactatc aaggaacaat
    15841 ggggcaagat ggggctctct gtagactact ctcgtgagcg tttcactctt gacgaaggtt
    15901 tgtcaaaagc tgttcgtaag gtctttgtgg acctttacaa gaaaggctgg atctaccgtg
    15961 gtgagtttat catcaactgg gacccagcag ctcgcacagc cctttctgat attgaggtga
    16021 ttcacaagga tgtagaaggt gccttctacc acatgaatta catgctggaa gatggttcac
    16081 gcgcccttga agttgctaca actcgtcctg agaccatgtt tggggacgtt gcggttgcgg
    16141 ttaatccaga agacccgcgc tacaaggact tgattggtaa aaacgtcatc cttccaatcg
    16201 ctaataaact catcccaatc gttagagatg agcacgcaga tcctgagttt ggtactggtg
    16261 tcgtgaaaat cacacctgcc cacgatccaa atgacttctt ggttggccaa cgtcataact
    16321 tgccacaagt caacgtcatg aacgacgacg gaactatgaa tgagcttgcc tttgaatttt
    16381 caggcatgga tcgttttgaa gctcgtaagg cagtcgttgc taagttggaa gaaatcggtg
    16441 cccttgtcaa aatcgaaaaa cgtgtccatt cagtcggtca ctcagaacgg acaggtgtcg
    16501 tagttgagcc acgcttgtct actcaatggt tcgtcaagat ggaccaattg gccaagaacg
    16561 ccattgccaa ccaagacaca gatgacaagg tcgaatttta cccaccgcgc ttcaacgaca
    16621 ccttcctgca atggatggaa aatgtccacg actgggtgat ttcccgtcag ctctggtggg
    16681 gtcaccaaat ccctgcttgg tataatgaag caggcgaaat ctatgtcggc gaagaagctc
    16741 cagaaggtga cggatggact caggacgaag acgtcttgga tacgtggttc agttctgccc
    16801 tctggccatt ttcaaccatg ggctggcctg aagtcgactc agaagacttt aaacgttatt
    16861 tcccaacttc aaccttggta acaggttacg acatcatctt cttctgggtg tctcgtatga
    16921 tcttccagtc attggaattc acaggccgtc aaccattcca aaacgtcctt atccacggtc
    16981 tcattcgtga cgagcaagga cgcaagatgt ctaagtctct cggtaacggg attgacccaa
    17041 tggatgttat cgagaaatac ggtgccgatg cccttcgatg gttcctttca aacggttctg
    17101 cgccaggaca agacgtgcgt ttctcttatg agaaaatgga tgcttcatgg aactttatta
    17161 acaagatttg gaacatttct cgctatatcc tcatgaacaa tggaggtttg acgctggatg
    17221 tggcgcatga caatgttaca aaagttgcaa caggtgaggc tggtaatgtg acggaccgct
    17281 ggattctcca caatctcaac gaaaccattg caaaagttac tgaaaacttt gataagttcg
    17341 agtttggtgt ggctggtcat atcctttaca acttcatttg ggaagaattt gccaactggt
    17401 atgttgagtt gaccaaggaa gttctttaca gcgacaatga agacgataag gtaattactc
    17461 gatctgttct tctctatacg ctggataaaa tccttcgtct ccttcactca ataatgccgt
    17521 tcgtgacaga agaaatattt ggtcagtacg ctgaaggctc tatcgtgaca gcagcatacc
    17581 caactgtcaa cccagccttt gaggaccttg ctgcccacac tggtgtggaa agtctcaaag
    17641 acttgatccg tgccgttcgt aatgcgcgtg cggaagtaaa tgtagcacca agcaaaccta
    17701 tcacaatcct tgttaagact agcgatagtg acttggaagc cttctttaac agcaatgtca
    17761 actacatcaa acgctttaca aatccagaac atttggaaat cgcatcaacc atccctgcac
    17821 ctgaactggc tatgtcaagc gtcatcacag gagcagaaat ctacttgcca ctggcagacc
    17881 ttctcaatgt cgaagaagaa ctggctcgtc tcgacaagga actcgctaaa tggcaaaaag
    17941 aactggatat ggtcggtaag aaactctcta acgaacgctt cgtagccaac gccaaaccag
    18001 aagtcgtcca aaaagaacgc gacaaacaag ctgactacca agccaagtat gatgcgaccg
    18061 tagcacgtat tgatgagatg aagaaactcg ttaaataaac acagaaacac ggtgatgagc
    18121 cgtgtttttt tggtataatg aaatcaatat ctattgatgg agaaagtgat gtccaaacac
    18181 cctcattatg aattgttaaa tttaattggc tacggtcttg ccaagtttga caagcttttt
    18241 ataaaagaat ttcaatgctc tagcaagtcg gagttttatc gctatgtggt ttctctggga
    18301 attgctgaaa caactggagt tgtaaaaaat agaatggatt tatttgatcc ttattttgac
    18361 aataatcgaa aaggttggtg gcagaaagct gaagtttatc gttttcgtaa agatttaatt
    18421 gatatgatgt ttggaaatga agatgttcat agttatgctg aaataattaa aatgttactt
    18481 gccagtgaag ggaagaaaac aggcataacc atcgttgaaa aaccaatagt tcgaactaaa
    18541 ttcaaacgtc tacaggaaac gggaatggag gctgagaatt attttatcct tcattttgat
    18601 aaagaagaaa aattccaagg tggacagtta accgatgccc gtctttatgg tgatggatat
    18661 gactttcagg tagatgtcca agagtattct taccttgccg aagtaaaagg tatccgaaaa
    18721 tctaaggggc gtgtgcgttt aactgcaaaa gaatttgaaa aagtcaagga gtttcaatcg
    18781 gattttattt tatccttggt cacaaatcta gatgatattc caaagttagt gttaatcgat
    18841 aatcctttaa aacattttga gtttaaaaag aatattatca aaaatgaaat cattgaatat
    18901 agaagcttag aagatttgta ttagttcccc ccttaacttt tccagttgaa agaacgagaa
    18961 gtcttgacaa gtcaacgact caatgaactg gaaatcaacg gtattcgttt aactaaattt
    19021 aaaaatgagg aaatcgtatt gaatttatct ggattgatac cgaaaatccc ccaagcgatg
    19081 ccatcggctg ggtagcaaag aaataaaagt tttcaaaggt attgtaacaa ataagggatt
    19141 ttattttcgt gtacaatttc agctattctt gttataatca gcctatagga atcaaggagg
    19201 tgactcttat ggctgttttt gtgtctttgg atggaattgt ggtagaaata cttgatgtct
    19261 ttttttcttt taatggggat agtgagtttt tcttgtgtat agcattttga agctggaata
    19321 ggacgccatg actgctaaaa gatttctata aattaatttg attttcctaa tcaatttgtt
    19381 catatcttat ttcattccac tgtaaacgtc ttaaagacaa gagtcagttt gtgatggaac
    19441 gctctcggtt cgaggagatg ttccaacttc aaagtagtca cttgacgacg caagaaaaat
    19501 cacaattgtt tacctccgtg tttgctggac gttatgatgt ttatgctaag aattttatca
    19561 atgaacaagg gaaaattcag tattttcctt cctatgatta tggttggaag cagttgccac
    19621 ctgaaaaacg gagtttccag acattgacga actccgtttt gaaatctcat tttcgtgggg
    19681 aggcagctat cggtatcttt cctatgcact tagatgatag ctgttatttt ttggtactgg
    19741 atttggatga aggagattgg aaagaagctg gtttaaccat tcgaagaata gccagggaac
    19801 gccagatgga agcccattta gagatttctc gttcgggtta cggactccat atttggttct
    19861 tctttgagga agcgattctg agtcgaaagg ctcgcctgtt tggaaagaaa ctattagaat
    19921 tggctatgca ggaaagtatg caactgtcct ttgattcttt tgatcgcatg tttccaaatc
    19981 aggatgttct tcctaaaggt ggatttgata atttaatttc ccttcctttt caaggagaag
    20041 cttatcatca agggcgaacg gtctttgtgg atgaacagtt tcagccttat gaagatcaat
    20101 ggaggtatct acaagaaatt cagaagattt caactgctaa agtggcactg ttaatccaag
    20161 aggagttagg caagcaagaa ttggataagg agttgaaggt cgttttatcc aatatgatcc
    20221 aacttaaaaa atcgtctgtg acacccaaga cactgttttt cttgaaaaat atggcttcat
    20281 tttctaatcc cgaatt
//
LOCUS       DAVKJJ010000036        20180 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000036 DAVKJJ010000000
VERSION     DAVKJJ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20180
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.036"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(<1..311)
                     /locus_tag="VW144_001391"
     CDS             complement(<1..311)
                     /locus_tag="VW144_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9600833.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRYQAGQDKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     EL"
     gene            complement(469..2919)
                     /gene="phtA"
                     /locus_tag="VW144_001392"
     CDS             complement(469..2919)
                     /gene="phtA"
                     /locus_tag="VW144_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEU9600834.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQTSQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGDHYHF
                     IPYSQMSELEERIARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSN
                     SSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKE
                     NVAPRDQEFYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDL
                     LAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYV
                     TPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVK
                     GEKRIPLVRLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLF
                     ATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVP
                     QVETEKVEAQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKL
                     LALLKGSNPSSVSKEKIN"
     gene            complement(3172..4905)
                     /gene="ptsP"
                     /locus_tag="VW144_001393"
     CDS             complement(3172..4905)
                     /gene="ptsP"
                     /locus_tag="VW144_001393"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEU9600835.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            complement(4911..5174)
                     /locus_tag="VW144_001394"
     CDS             complement(4911..5174)
                     /locus_tag="VW144_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEU9600836.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            5525..5743
                     /gene="nrdH"
                     /locus_tag="VW144_001395"
     CDS             5525..5743
                     /gene="nrdH"
                     /locus_tag="VW144_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEU9600837.1"
                     /translation="MVTVYSKNNCVQCKMTKRLLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            5824..7983
                     /gene="nrdE"
                     /locus_tag="VW144_001396"
     CDS             5824..7983
                     /gene="nrdE"
                     /locus_tag="VW144_001396"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEU9600838.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            8171..9133
                     /gene="nrdF"
                     /locus_tag="VW144_001397"
     CDS             8171..9133
                     /gene="nrdF"
                     /locus_tag="VW144_001397"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEU9600839.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            9315..10076
                     /locus_tag="VW144_001398"
     CDS             9315..10076
                     /locus_tag="VW144_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9600840.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNISNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(10361..10612)
                     /locus_tag="VW144_001399"
     CDS             complement(10361..10612)
                     /locus_tag="VW144_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEU9600841.1"
                     /translation="MFKDFIQSIYEKVYIINFEKCSQMPCLTSEELKSLGKWYVSTGK
                     EWICHSDADLEEFKNLFLNFINPEEWDTISFDSDFMPFQ"
     gene            complement(10655..12061)
                     /gene="lacG"
                     /locus_tag="VW144_001400"
     CDS             complement(10655..12061)
                     /gene="lacG"
                     /locus_tag="VW144_001400"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU9600842.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            complement(12276..13970)
                     /locus_tag="VW144_001401"
     CDS             complement(12276..13970)
                     /locus_tag="VW144_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9600843.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIF
                     ILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTN
                     QINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNN
                     VTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVDKILAPLFS
                     ATDGYIGLAIVFGAYAFFWFVGIHGPSVVEPLIVAISYANIEANVQLVQAGMHADKIL
                     NPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSF
                     VLAITLIIIDTLIYYPFVKVYDHQILEEERKGNSSSELKEKVAANFNTAKADAILEKA
                     GVDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLP
                     EFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            complement(13972..14289)
                     /locus_tag="VW144_001402"
     CDS             complement(13972..14289)
                     /locus_tag="VW144_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9600844.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            complement(14325..15161)
                     /locus_tag="VW144_001403"
     CDS             complement(14325..15161)
                     /locus_tag="VW144_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9600845.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            complement(15395..16375)
                     /gene="lacD"
                     /locus_tag="VW144_001404"
     CDS             complement(15395..16375)
                     /gene="lacD"
                     /locus_tag="VW144_001404"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEU9600846.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            complement(16377..17306)
                     /locus_tag="VW144_001405"
     CDS             complement(16377..17306)
                     /locus_tag="VW144_001405"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEU9600847.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQEFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQDPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            complement(17317..17832)
                     /gene="lacB"
                     /locus_tag="VW144_001406"
     CDS             complement(17317..17832)
                     /gene="lacB"
                     /locus_tag="VW144_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEU9600848.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHHESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            complement(17863..18288)
                     /gene="lacA"
                     /locus_tag="VW144_001407"
     CDS             complement(17863..18288)
                     /gene="lacA"
                     /locus_tag="VW144_001407"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEU9600849.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            complement(18445..18921)
                     /locus_tag="VW144_001408"
     CDS             complement(18445..18921)
                     /locus_tag="VW144_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEU9600850.1"
                     /translation="MPYFIGGHPGFNCPLLDDGVYEDYYLESEKEETCSVPRPFPETG
                     MLDFQDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFP
                     NLIIWSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            complement(18958..19761)
                     /locus_tag="VW144_001409"
                     /pseudo
     CDS             complement(18958..19761)
                     /locus_tag="VW144_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(19769..20071)
                     /locus_tag="VW144_001410"
     CDS             complement(19769..20071)
                     /locus_tag="VW144_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9600851.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFCIPAMSVPVFDNIILPAKQNMV"
     gene            complement(20109..>20180)
                     /locus_tag="VW144_001411"
     CDS             complement(20109..>20180)
                     /locus_tag="VW144_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9600852.1"
                     /translation="YLEDLSKISEPEKLYAYIAEATA"
BASE COUNT         5985 a         4150 c         3583 g         6462 t
ORIGIN      
        1 agctcttcac tgatgatggc atcataagga accttgccat tatagtaatg ataatggtct
       61 ccatgagagg tcacataacc ttgatccgta atcttgataa caatttgttc ggcgttgatc
      121 ccctccctct tactgacttc atctggtgtc aagttttctg ccttttgacc agcctgatca
      181 ccatctatat aagcaactcg attagactct ttcttatcct gaccagcttg gtaacgtcca
      241 agctcatagg aacaaacact tagggcaagg actgccactg aacctgctag atatttttta
      301 ttgattttca ttctttcctc ttttcttaag tatatttgat tcactataaa gtaataaacc
      361 ggttaattaa ctagttaata gtttactctt tttaatattg cttgtcaaga attttacata
      421 tatacataat aaaaatgaaa gcctctttat cgagactttc atttttcatt agtttatttt
      481 ttccttactt acagatgaag gattacttcc ttttaacaac gcaagtaatt tttctgcttc
      541 tgccatgata ctattgttat ccataatttg aagagtcaaa ttatttcgta aaccagctag
      601 agtttctgtt gcattggctt tcagactaga atccgttact ttcgcaagca aaacttctgc
      661 ttctttgagt tgggcttcta ctttttcagt ctctacttga gggacttctg gctcagcagg
      721 tgtttcctct actggctctt catccgcttt gaagttctta tttggatctt cactgtggtc
      781 tttcttgcct aacacatgct cactggcatt gccccatcca tcattagaat gtggacgttc
      841 gtcagggtgt tctacgtagt acttaatcgt cgcaaacaaa tcttccaagg tatagccatt
      901 tggagctttg tatgtgtgat catcaaacca agcaaattta atgttatggt aatgatcctt
      961 atgaggaata atcaaattac cgtttttaac ctcaactgta tgctcaacca tatatggaag
     1021 tcgaacgagt ggaattcgtt tttccccttt cacacgattg taaatagctg ctgcactatc
     1081 tccagttgga tttgctttaa catctgcgtc tggagatgga ggtaggatac ctttttcttt
     1141 agtataggct tgagctgcaa ctttttcctt atcagaaagg ctgtcttttc caatccagtg
     1201 actatggccc atatgaggcg ttacatatgc atctccttca tcactgatta tatcatgttc
     1261 atcaaaaata taaccatctg acgttgtata cttatcagct aattgagcaa tacgaacttc
     1321 gtcttcagta tactcaattt gagaatttgg cttgccaagt cgctctggat gggtaattgg
     1381 tgctaggaat gccaataaat catctaccaa tttttcttta ttagtcgatt catcattcaa
     1441 gcgttctaat aatttgtcta aggcttggaa atcagaatta cgacccttat tttcaaacaa
     1501 ggctttatga gcctcagtta acagattata tgctttatca taaaattctt ggtcacgagg
     1561 agcaacattt tcttttttag cagttaaagt gtgtgaaaca ctctcttgtt ttgataactt
     1621 gctttcaaga tttttaacag tttcagatgg taaatctttc gcaaagacat aacgagagat
     1681 gcccttttct tcgaatacat atccttcccc aacttttcgt accagctgac taaccaaaga
     1741 agaatttgag tctattttaa gatttggtgc aggttgcggg cctggactag gttccggagt
     1801 cggttgtgga cttggttgtt ctggccttga atctggtacc caatggtttg aacgataacg
     1861 aaggggaata atacgagcga ttcgttcttc caattcagac atttgagagt aagggatgaa
     1921 gtggtaatga tctccgtgtg gcactgcaac acctctagct gttcgacttg tgatttgcgc
     1981 tgggtcgaaa acaaggccat cagattctac atgtcgttga ctcaaaggca gtttgtagag
     2041 ctgtttcaag agactatcaa tgtcattact ttgacttgtt tgactgttag tgttgctgtt
     2101 gttgcttgtg ttagtatttg tagttcctgg attgcttaca gaaggtaccc agtttgttct
     2161 tgaagtgtta tcgctatttt gtcggcgata ggttcttgaa tttgacagat ttccccgacc
     2221 agataggaag gcttctgcag cagccaactc gctagctgat aactcattct taggaatgta
     2281 atggtaatga tctccatgag gaacgatata agcatcacca gtatcctcta tgatatcaga
     2341 agcattaaag atataaccat catctgtagt atagcgtcct tgcgaacgtg ccaaggcaac
     2401 agcaccatcg tttcttggag tcccaccttc acgatgttga ctatgttctt gtttttgtcg
     2461 attgatttcc tcttttgtac ggacgttatc cgcgtgggca gcatccttaa ggtaaacata
     2521 gtattttcca tctaccttga taacatatcc acccttgacc tcattaacaa tatcctcatc
     2581 ttttagctta tagttcggat ctttcatgag taattcttca ctgaaaatcg catcataagg
     2641 aaccttacca ttgtaataat gatagtggtc gccatgtgaa gtgacatagc cttggtctgt
     2701 tatcttgatg acgatttgct cagcattgat tccttcacgc ttgctaacct catcaggagt
     2761 caaattctcc gttttttgcg tcgcttgttt tccatctata taggaaacac gattattttc
     2821 cttaaccgtt ctagcttgat acagtcccaa ctcgtaagaa caaacactta aaatcaaagc
     2881 tgccgcagaa ccaacaaggt atttcttatt aattttcatc gaaactctct ttctatttga
     2941 tatttctagt tgcaattcat ttctaaattg cagaaactcc tctctattac ttaactggtt
     3001 aatagtttac tcctaaatct ccaacttgtc aagaatttta tgaaccattt aagtattttt
     3061 tgttctgact actacttttt caagaaaatc tttctataag aaagattttc ttgaaaattc
     3121 tttttaaaat atcaaaaaaa tccctgtaac tgagttgcag ggacttttcg attaatcaaa
     3181 attaacgtat tctttttgaa gttcaagaac ttcttccatt gttgagcatt ctgtaagggc
     3241 acggtttgcg tactcttcca tcttagctgt gtcgagtttc ttcatcaagc tgcgtgtacg
     3301 aagtacagat gttgctgaca tagagaactc atccaagccc attccgacaa gaagtggaac
     3361 agcttgttgg tcaccagcca tctcaccaca cataccagcc catttacctt cagcgtgagc
     3421 tgctttgatc acattgttaa tcaagcgtag gattgatggg ttgtatggtt ggtaaaggta
     3481 tgaaacttgt tcgttcatac ggtctgctgc cattgtatat tggatcaagt cgtttgtacc
     3541 aattgagaag aagtcaactt ctttagcaaa ttggtctgca agcatagccg ctgcaggaat
     3601 ctcgatcatg ataccaactt ggatgttatc cgcaactgca acaccttcag caagaaggtt
     3661 tgctttttct tcatcaaaga ctgctttcgc tgcacggaat tctttcaaga gcgcaaccat
     3721 tgggaacatg atacgcaatt gaccgtgaac agacgcacga agaagagcac ggatttgtgt
     3781 gcggaacata gcatctccag tctcagagat agagatacga agagcacgga atccaaggaa
     3841 tgggttcatt tcgtgaggca tatcgaagta aggaagttcc ttatctccac cgatatccat
     3901 tgtacgaacg acaacaggtt taccgttcat tccttcaaga acagccttgt atgcttcata
     3961 ctgctcatct tcagttggga agtcttgaga atccatgtac aagaactctg tacggtaaag
     4021 tccaacagct tctgcaccgt tgttgttaac accttcaacg tcttttggag taccgatatt
     4081 agcagccaac tcgaagtgtt taccgtcagc agtcactgtt tgagcatctt tcaagagcgc
     4141 ccattcagct ttttgtttcg cataagcttc accagctgct ttaaattctg ccgcttgttc
     4201 atcagttggg ttgataataa cctcaccagt gatcccgtta acagcaagga tgtcaccgtc
     4261 tttaacgatt tcagtgatgt tgtttgtacc taatacagct gcaatttcaa gtgtacgtgc
     4321 catgatagct gagtggcttg tacgtccacc gatgttggtt acaaaagctt ttacaaagtt
     4381 tttgtccaat tgagctgtat cagatggtgt caagtcatgt gcaatcacaa tcacttcttc
     4441 attgatagaa gctgggtttg gcaatttttt accaagaagg tttgccaata cacgttttgt
     4501 cacatcgcgg atatccgctg cgcgttcttg catgtatggg ttgtcttcca tgccttcaaa
     4561 gatagtgata aacatatctg taacttcttt cagacctgct tctgcattca ctttcttcgc
     4621 acggatagtt tccttgattt ggctgatcat ttctgggtca gcaagaacca ttaagtgagc
     4681 atcaaaaact tgagctgctt cttcaccgag cgtacctact gctttctcgc gaataacaga
     4741 aagctcgtct tgtgatgcct gtagagcggc atcaaggcga gcttcttctg cgtttgtatc
     4801 ttcgactgta atagtctcaa atgacaaatc cggctgaacg agtagatatg cttttgcaac
     4861 tgcaacaccg tcagatgctg cgattccttt aagcatttct gtcatttccc ttatgccaat
     4921 ccttcttttt ccattgtttc tgagattgca gcgatagcgt catctgcatc tgcaccttca
     4981 gctgagatag ttacgtcagc accttggcca acaccaagac tcataacacc cataattgat
     5041 ttaaggttaa ctgatttacc tttgtactca agagtgatat ctgaagcaaa tttgctagca
     5101 gtttgtacca acaatgttgc tggacgtgcg tgaatacctg tttctgccac tacgtggaaa
     5161 tctttagaag ccatagtttg actctccttt tgttctttct ttttttgagt tatatgtgat
     5221 aacccttaca atttagtatt ataccatctt ctaggatttt tttcaagcat tttctggatt
     5281 ttccttcaat ttcctttcag ataacttgct gaaaatcttc tatcctagat aacaaaacac
     5341 aggatatagt ttctttaaaa acaatttata ggataatcat tgctatttca caatacaaac
     5401 actatatatt gtgtttatat ataaaaacaa caaaagaaca ccactatatt tagttcaaat
     5461 cattgacaaa attttatttt ctgatatact aagatagtat taaattttga agaggagtta
     5521 cacaatggta accgtttatt ctaaaaacaa ttgtgtccaa tgtaaaatga ccaagcgttt
     5581 attggacagt aataatgtct cttatcgtga aatcaatctt gatgagcaac ctgagtacgt
     5641 cgatcaagtt aaagagctcg gttttagcgc agctcctgtt atccaaacac caactgaagt
     5701 cttttcaggt ttccaaccag gaaaactgaa acaattagca taatcttagt acatcatcca
     5761 gaagaaactg cttttagggc taacttagaa gcctttcttt tgtaattaga taagggaaat
     5821 tttatgggat taaaacatct tgaagacgtg acttacttcc gtcttaataa cgaaattaac
     5881 cgtcctgtta atggacaaat catgcttcat aaagataagg aagccttgga tgctttcttt
     5941 aaagaaaatg tagttccaaa cactatggtt tttgattcaa tcaaagacaa aatcaattac
     6001 ctcattgaac acaactacat cgaaacagcc tttatcaaga aataccgtcc agagttcttg
     6061 gaagaattgg ctcaatttat caaagaccaa aacttccaat tcaagtcatt catggcagct
     6121 tataaatttt acaatcaata tgccttaaag actaatgacg gtgaatacta tcttgaaaat
     6181 atggaagacc gtgtcttctt caacgccctt tatttcgctg atgggaatga agctgttgca
     6241 atcgatattg ccaatgaaat catccaccaa cgctaccaac ctgctactcc ttccttcttg
     6301 aatgctggac gtgctcgtcg tggggagttg gtatcttgtt tcctaatcca agtgacggat
     6361 gatatgaact ctatcggacg ttctatcaac tcagctcttc aactttcacg tattggtggt
     6421 ggtgtgggaa ttaccctcag caaccttcgt gaagctggtg cacctatcaa aggctatgaa
     6481 ggagctgctt caggtgtcgt tcctgttatg aagctttttg aagacagctt ctcttactcc
     6541 aaccaattgg ggcaacgtca aggtgctggt gttgtctacc tcaacgtctt tcacccagat
     6601 atcatcgctt tcctttcaac taagaaagaa aacgctgatg aaaaagtacg tgtcaagact
     6661 ctatcacttg gtgttgtagt acctgataaa ttctacgaat tggctcgtaa aaatgaagaa
     6721 atgtacctct tcagccctta ctctgtagaa aaagaatacg gtgtgccatt caattacatc
     6781 gacattactg aaaaatacga tgaattggtc gcaaatccaa atattcgcaa gacaaaaatc
     6841 aaggcgcggg atttggaaac tgaaatctct aaattgcaac aagagtctgg ctatccttat
     6901 gtagtcaaca tcgatacggc taaccgtgca aatcctgttg atggaaagat tatcatgagt
     6961 aacttgtgtt ctgagattct tcaagttcaa gaaccaagct tgatcaacga tgctcaagaa
     7021 ttccttcaaa tgggaacaga tgtttcatgt aatctgggtt caaccaacgt ggttaacatg
     7081 atgacttcac ctgattttgg tcgttctatc cgtgctatgg ttcgtgccct tactttcgtt
     7141 acagatagtt cacatatcgt agctgtacca acgattgacc atggaaatag ccaagcccac
     7201 acctttggtc tcggtgccat gggactgcac agctaccttg cccaacaact cattgaatat
     7261 ggttcacctg agtctgttga atttacaagc atctacttta tgcttatgaa ctactggacc
     7321 ttggtagagt caaacaatat tgcgcgtgaa cgtggtatta ccttccacaa ctttgaaaaa
     7381 tcagactatg ctaacggaag ttactttgac aagtatgtaa ctggtgaatt tgttccaaca
     7441 tcagaccgtg ttaaagaact cttcaaaaat gtttttatcc ctggtgttgc tgattgggct
     7501 gaacttcgcg acaaggttca agaagatggt ctttaccacc aaaatcgcct tgctgtagcg
     7561 ccaaatggtt ctatcagcta tatcaacgac gtttctgctt ctatccaccc gattacgcaa
     7621 cgtatcgaag aacgccaaga gaagaaaatt ggtaaaatct actaccctgc tgctggcttg
     7681 tctacagaaa ccattcctta ctacacttct gcctacgata tggatatgcg taaagtcatc
     7741 gatgtttacg ctgctgcgac tgaacacgtg gatcaaggac tttcactcac cctcttcatg
     7801 cgcagtgaca ttccaaaagg cctttacgaa tggaagaggg aaaataaaca aacgacacgt
     7861 gatttgtcca tccttcgtaa ctatgccttt aacaagggaa tcaagtctat ctactacgtc
     7921 cgtaccttta cagatgacgg tggagaagtc ggcgccaacc aatgtgaaag ctgtgtgatt
     7981 tagtttctag catagaaaca ttatttttcc ctaagcagca ctgataaaat caaatctact
     8041 atgtcataac ataatttatg taacatttta aaagtcgggc ttccgacttt ttgtgcgata
     8101 ctcctataga attatggtaa agtagaaata attgtgagtt cgcaatacta aacacagaaa
     8161 gagattttaa atggaaactt actacaaagc cattaactgg aatgccatcg aagatgtcat
     8221 cgacaaatca acttgggaaa aactgacgga gcaattttgg ctcgatacac gtattccctt
     8281 atcaaacgac ttggatgact ggagaaagct atctaacaaa gagaaagact tggtcggaaa
     8341 agtttttggt ggtttaaccc ttcttgacac tatgcaatct gaaactgggg ttcaagccct
     8401 tcgcgcagac atccgtacac cacatgagga agctgttttc aataacatcc aatttatgga
     8461 atctgtccac gctaaatctt actcatcaat cttttctacc ttgaatacta aggctgagat
     8521 tgaagaaatt ttcgaatgga ccaataccaa tccttaccta caaaagaagg ctgagattgt
     8581 caacgaaatc tacctaaacg gcagcccact tgaaaagaaa gttgccagcg tcttcctcga
     8641 aaccttcctc ttctactctg gtttcttcac tcccctctac tatctcggta acaacaaact
     8701 agccaacgtt gcagaaatca ttaaattgat tattcgtgac gagtctgttc acggaaccta
     8761 cattggttac aaattccaac ttggtttcaa tgaattgcct gaagaagagc aagaaaaact
     8821 caaagaatgg atgtacgacc tactctatac tctttatgaa aatgaagaag gctacacaga
     8881 gagtctttat gacggtgttg gttggactga agaggttaaa accttccttc gctacaatgc
     8941 caataaagct ctcatgaaca tgggacaaga tccacttttc ccagattcag ctgaagatgt
     9001 caacccaatc gttatgaacg gtatttcaac aggaacttcc aaccatgact tcttctctca
     9061 agtcggaaat ggttaccttc ttggcgaagt tgaagccatg caagacgatg actataacta
     9121 cggtttagac taattctcca tcactctcaa tcatcaaaaa gtaaactaga aacatcaaag
     9181 ttataaccca acaaaaatat cgaaagtttt gaaaacaaac ttcgatattt ttgtttttgt
     9241 ttcattttgt tgtttttgta tttgaatgat gagtttgttt atggtaagat agtagtagga
     9301 aacgaggtga taatatgctc aaacaagaaa aattaaccaa aattttagag atagtaaata
     9361 gtaaaggaac cataacagtt aaacagatta tggacgaaat agccgtttca gatatgactg
     9421 caaggcgcta tttacaggaa ttagctgata aagatttgct gattcgtgtg catggtggag
     9481 ctgaaaaact tcgaaccaac tcccttttga ctaatgagcg atcaaatatt gaaaaacaag
     9541 ccctccaaac ggcagaaaaa caagaaatag cccattttgc aggcagtcta gtagaagaaa
     9601 gagaaactat tttcattgga ccaggaacaa cattagagtt ttttgcgcgt gagttgccta
     9661 ttgacaatat ccgcgtcgta accaacagtc tacctgtttt tctgatttta agcgaacgaa
     9721 aattaacaga tttgatttta ataggtggaa attatcgcga tattacaggt gcttttgttg
     9781 gtacattgac cctacaaaat ctctctaatc tccaattttc taaagctttc gttagctgta
     9841 atggtattca aaacggagct ctagctactt ttagcgagga agagggagag gctcaacgca
     9901 tcgctttaaa taattctaat aaaaaatatt tactcgcaga tcatagtaag ttcaataagt
     9961 ttgattttta tactttttat aatatatcaa atcttgatac tattgtttca gattctaaac
    10021 taagtgattc aatccttttt aagctatcta aacacattaa agtcatcaag ccttaataaa
    10081 ctgaaagagc ctgggtaaca actcaatttc agaagaaaaa aagtaaatct tcccacaata
    10141 aaaagcatag tatcaggtgt tgaaacacct gatactatgc ttttttctat atcaaagact
    10201 ttttgcccaa ttcctcaaag ctatacattg agaaatctgc gactcgcttc ctagtttaaa
    10261 gttgctcttt gattttcatt gagtataagc aacgtgatta gataagagtt ttacagagta
    10321 ctaaatataa gatttaactt ttttgagaaa ttggttacga ttattgaaac ggcataaaat
    10381 ctgaatcaaa ggagatagta tcccattctt caggattgat aaaatttaaa aatagatttt
    10441 taaattcttc cagatcagca tctgaatgac aaatccattc tttaccagtc gagacatacc
    10501 attttccaag acttttcaat tcttcactcg tcaaacacgg catttgagag catttttcga
    10561 aatttataat ataaactttt tcataaatag attggataaa atctttgaac atctttttga
    10621 cttactaaaa ttctatatct aatgactaat tctactagtc tataatctga gtttccgcta
    10681 ctttcttgta ccagtgagct gatttcttag gataacgttc ttgagtttca aaatctacgt
    10741 agaagagacc ataacgtttc tcataaccgt ttgaccatga gaagacatcc attaatgacc
    10801 aaatgaagta accttttaca ttagctccat ctgcaatcgc atcagacaag atctccaagt
    10861 gttgtttcac gtaatcaata cgaccatcat cgtaaacagt gttatcaacg aactcatctt
    10921 tatagccgag accattttca gtgatgtaaa tcttcttgta gttaggataa tctttcttca
    10981 cacgcatgat ttggtcatac aaaccttgag ggtagataat ccaatcccaa tccgtgcgtg
    11041 gtacatagtc aggagctaca cgacgaccaa cacctttgat ttgatactta gagcttcctt
    11101 tttcaccttt accattatgg ataatttcag tttctccatc aaaggcttcc atccagtcac
    11161 tcatatagta gttgattcct aggaaatcat tcaagtcttt tgcagcttct aatgctgtaa
    11221 aatcttcttc acgaagatcc aaactaccac cattgactga taagatatgg ttgacacctt
    11281 ccatggtttc agctgaatag cgacctagat aagttgcgtc taagatgaat ttattgtgga
    11341 tgatatcttc caactcagct gcacgaacat ctgctggatt ttcaggatct agaggatatt
    11401 tagtaggcag ggcgtgaaca acaccaattt cccctttata gcctttctct ttgtacaatt
    11461 ttaccgcgcg tgcatgagac accatcatat tgtggtgtga ttgaaagact ttggcaaggt
    11521 cgtactggat acctggaggg aatttcccaa ccaaatattg accatcaccg attggtccaa
    11581 tttcattaaa ggttgtccaa tagtttactt ctggaaattc ttcaaaacag aaggcagcgt
    11641 agtctacaaa atgttcgata ttttcacggt ttaagaagtc tccatttgag tggagagctt
    11701 ctggcgtgtc aaagtgatga agagttacaa aaggctcaac atgacgttta tgacactctg
    11761 caaataaatt atgataaaac tcaacacctt tagcatttac ttggccgtaa ccagtcggga
    11821 aaatacgtga ccaagcaata gaaattcgaa taccattgac accatactct tctgctagct
    11881 tgaggtcaac tggatatcga ttgtaaaaat cactagctgg ttcggcagtg taccagtagt
    11941 tatcctcaag atatttatcc caagcaactg gtccttttcc atcagtatgt gtagcacctt
    12001 ctgcttgata agcagctgtt gcgccaccga aaataaagtc ttttggaagt gtttttgtca
    12061 tttttttcac ctatttcttg atttaaataa agtaaaagtg agaggagagg agtcagtgag
    12121 ccatgcttct ccatctcact tcttgtacca agtcactgaa ttgtgacatg aggaggtaaa
    12181 aacgatatct ttttaccgac gaattaataa ggcgattagg aaattcgcca tagcgatttc
    12241 cgtaacgatg ggagactgtt tcacagttcc tagccttaat cgaattgctc ttgtacgaat
    12301 gctagggcac cttttccatc acgagttaat ttgatgtatt gagcgccttc tgtcttagca
    12361 agtttaatac cgagcttatc tgtttctgcc ttcatatctt caaagtttga agcaacttga
    12421 ggagcaagga taaccaaatc aaactctggc aacatttcac ggtgagcacc atagccgcct
    12481 gctgctgctt tcacagggac attgtattct gctgctgcct tattcaaagc atttgcaagg
    12541 agaccacttg tacctccacc tgcacagaga acgaggacat ttgtttcttc agtgatggta
    12601 ttttgtgctg catcgacacc cgctttttca agaatagcat ccgcttttgc agtgttgaag
    12661 tttgcagcaa ctttttcttt caattcagat gaagagtttc ctttccgttc ttcttctaaa
    12721 atttgatggt cataaacttt aacaaatgga taatagatta aagtgtcgat aatgattaaa
    12781 gttatagcca agacgaatga caatggagcc agcgccgttc ccataacaat accaattgga
    12841 gcaggtgttg tccaaggtaa aataatactg aaactattca tttgtagaac atctacaaaa
    12901 aatttcataa tccaaacatt gataatagga gcagtaacaa acggaataaa gaagatagga
    12961 tttaaaacaa ttggagcacc aaataggatt ggttcattta cgccaaagaa agtaggaact
    13021 acagacgcac gtccgacaat tctatttctt tttgatttac ataaccacat aaacataaat
    13081 ggaactacta gtgttgcacc agtaccaccc attgttacaa caaaagtttg agtaactgga
    13141 tttaaaattt tatctgcgtg cataccagct tgcacaagct gaacattagc ttctatattt
    13201 gcataactga tagctacaat taaaggttct acaacagatg gcccatgaat tccaacaaac
    13261 caaaagaaag cataagcacc aaatacaata gctaaaccga tataaccatc agtagctgaa
    13321 aatagagggg ctaatatttt atcaacagat tctgctacat ttgtaccaaa aatatttcta
    13381 acaattaaat ccaatccata tattgaaaga acagataagg caaacggaat aacatcttta
    13441 aaaacttgtg caatatttgg tggaacctct tcaggaagtc taatggttac attattttta
    13501 atacatactt tatagatatt tactgtaata aaagctgcca aaaatgcagt aagtaatcct
    13561 ctagtaccta aaagaccatt agcaaatcca ccttcaattg gatcagctgc cagtattaac
    13621 aatccaacaa cagctgctaa aaaagttgac atgtagttga tttggttggt tttctccata
    13681 ctacgtgtat taactgaatc tgttaacgat tttgcagttg tcccagctac taagagagct
    13741 aaaatcccca ttgagtagtt gtaaggtttc aaaataaaag ctaactgttc atcagaccat
    13801 ttaaacccaa aaatattggg cacagcagct attagtataa aaatacttga gaataatact
    13861 gctggcatac tggaaataaa accatctttt atcgctctta agtatatatt tctcgataat
    13921 ttctcaaaga aaggctttcc tttctcaata aaaattatta acttatccat tttatttaac
    13981 tcctctcttg taaagttcaa ttaaatggtg cattaggtct tttaacaaga tagttgtcat
    14041 caagtgatct tgaccatgca tcatggtcac actatacgct aagtcctcac cagaagcttc
    14101 cttagtcaat aaacttgttt gcgcgtggtg agcctctgca atacagctac cagcttcctc
    14161 taccagagcg tccgcttttt caaaatcacc agcttcagca gccttcaagg cttccaatag
    14221 ttttgaacga gcatcgccag cataggctac gatttcaaaa cctaacaatg ttacttcttc
    14281 tctattcatg atagaattct ccttatatag ttttaaataa atttttatga caataaacgt
    14341 tggatatgta gaactagata aactcgttca cttttataca aatcaagacc cgtatgttgc
    14401 gtaatcacat catagatctt ggaaccaatc tcgaaggctt ttggatagga ttgtttaata
    14461 tgatcttcca tatccagaag tgattggtta tcatctctag atctgtctaa ataatccaag
    14521 aaataattca aatggatcat aaagcgatca tagaaatggt tattcttttt agttcgttga
    14581 attgcatagt ccgttaaaac ttcttcaaca ttcctgagaa tttctttcct cttatcaatc
    14641 gactccacaa gttccacttc attttcacct tcagcattaa tgaaatgata agcaatccga
    14701 ataatttcgt cctcaggaaa atgatctgct agcttctgac ggtaaatttc aaaagcttca
    14761 tttgcgattt gaaaagcgac aggatactta gcggaaatat ctggcagatt actatccttg
    14821 taccttcctt gagttagagc ttgataagaa cagtaaatat gatctgtcaa ggttacatag
    14881 agatactctt gaatcggata atgatatttc tttgatagct tatcaatgat ttcataggtc
    14941 actgtgataa aatcaagcgg aacatctttg agaagagcca taaagttttc tctggactct
    15001 tcggtcttca tccgaaagat tttctcaacc tgattttcag caatcaaatc ccccttcttc
    15061 tttccgaatg caattccctt accaattaca atcacctctt ctcccttatc atttctgaca
    15121 agcgagacat tgtgattcat tggatttaga attcgataca tgtcaacctc cttttatatc
    15181 ttaaaggtat ataagtacaa aaaatgacca caaatgaact agaaaatcag ctgatttcta
    15241 attcacctgt gatcatgcct aatattactt agtaacactc accttgtatt aacttgtgat
    15301 ttaagtatag cacaagtaag ttattttgta aacctttaca tacaactttt ttcaaatttt
    15361 tttagattca tgttcctaaa ctaggaggac tttcttacac acgttctttc catgaagtcg
    15421 ctgttgtttg aagaacttta ttgagctcat caatgttttc aaatccagtt gtgcgaagcc
    15481 attcgcgagc tgctgcttca ccatctttga tgtaagcttc aactgatcct gcccatgtag
    15541 cacggccaca aagaactccg ttaaagtttg cacctgattc atgagcaaat acaagagtat
    15601 cttggaagag tttagctgat acaccagcac tcaagtagat gtatggcaag ttcgttgctt
    15661 catcttgcgc tttgaagaag gctgctgctt cttcacgtgt ataaactact tcaccttcag
    15721 cgaagccttc aacatattta atgttaacag gaacttcaac tttcaagaca tcaatgttaa
    15781 agcgtgggtc tgaaaagact ttcatagcgc cgataacttt gtgtggtttt actttcgcgt
    15841 attctacaga acctgcatcc gcaatttttt catcgtaagc aaggatttca aggaagaatg
    15901 ggatatcttc agccacacac tcagaaccga tgcgttcgat gtaggcttgt ttttcttgat
    15961 tgagttcgtc tgagctatct acatcatagt aaagcaagaa tttaactgca tctgcacctt
    16021 cttctttaat acgttttgca gaccagacat ccaagcagtc tggcaagcgt tttgtgcttg
    16081 ttgtgtcata acctgttttt tcataagcaa ggagaagacc agctttttca tcaagagctt
    16141 tagttgctgg aagtccatac tcagggtcaa gaagcataga tgaagcgtat ttagtcaatt
    16201 catctgctac caagacttta agttcttcca tttgggccac agttggttct tctgtttggt
    16261 gttgagccat gaggcgtttc aaagcaccac gttggtcaaa tgcaagagct gagatgatac
    16321 cattttcatc agaaagtttt tctaagcgta cacgtttttg ttctgttaaa gccattttat
    16381 acctctttta ctattaattg atcatataga acttgatagt tggccatgtt gacatgacca
    16441 gtcatttttt cttgagcatt gagcatacca aggacatttg ccttgatgag taattctgca
    16501 tccgattctt tgtgaagaag tcctgaagaa attcctgcca cagtagagtc tccagatcca
    16561 acaggattta ccacctgaat tctaggaata tctaccttgt agaaagtgtc accatgtttg
    16621 gcaaaagtac cgttggcacc aagtgaaacg ataatccatt caatccctgc aaacaaaggg
    16681 tcttgaagta cttcttttaa ttcatccaaa tcctcagaaa cttctcttcc aagaagctga
    16741 gacaattctt cattatttgg tttgatgact gttggtttat ggggtgattc aagaacagcc
    16801 tgaagtgctg cacctgagca gtccaagact acaggcttgc cagcttgatt agcaagttct
    16861 accaagctcg catagtaatc aactggaagg ccagctggca gactacctga gatggccact
    16921 acttcaactg attccaagag ttttttgaaa tgttccaaaa actcttgacc ttcctgttcc
    16981 aatacttcag gacctttttc aagaacttct gtttggttgt ctccgtggag aatagcgata
    17041 cagttacgag tttctccctg aattgagaag aaatctttct ttacttgatt atcgatatgt
    17101 tcaaccaaaa actcaccaag tttgccaccc actaaaccag tagcaagaac agaatcacca
    17161 aattctgaaa gtactcgggt aacattgagt cccttaccac cagccgtttt ggttacatcc
    17221 accacacgat tgacagtatc aatcttcaac tcatccaagg gataggaaat atcaatggat
    17281 gggttcattg taactgttaa aatcataggt tacctcttag tcgtggtatt ctccgcgatc
    17341 ccatttttca aggaattcag tgaagaagtt tgcgtctgtt tgttgagcat tgtgactttc
    17401 atggtgttca attttcgcaa tcaatttttt gttttcttca gatggtttgt attcagcatg
    17461 aatgaaagct tcgatgatat cacacataag caattcacca gtaatcttac caccaaaacc
    17521 gataacgttg gcgttcaatt gttctttagc ataaagggct gttgtcatat cacgaaccaa
    17581 ggcagaacga acacctggaa ctttatttac agcgttgttg ataccaacac cagtaccaca
    17641 gatacatacc ccgagatcag cttgaccact agttactgct tcccctactt ttttaccaaa
    17701 gattgggtag tgagtccgtg tatggtcata ggtaccaaag tcaatgactt catatccttt
    17761 tgatttcaaa aattctgaaa ccgccatttt ttcatcagtt acgatgtggt cacatccaat
    17821 tgcaattctc atttttaact tacctttctt actgtaatct aattagccca ttttgttcaa
    17881 catgtcaacc cggatttgat gacgtccacc gtcgtattta ccattaacaa atcctttagc
    17941 gatgttttta gccaattcat caccaacaag ttgtgccccc atagtgatca tacgtgagtt
    18001 gttgtggcca cgagtcatat aagctgaacg ttcgtcagat acttctgcag caaccattcc
    18061 tttgatctta gttgcaacca taaatggacc agctccataa gcatcaatca cgataccaag
    18121 gttttgttct tctttgttta cttctgcagc aacagcgaga gtcacatcaa caaagtcttg
    18181 accttcagct gtaacatcca caaggtggaa gttttctttt tctaagaagt ctttcacaac
    18241 ttctttcaat ctcaaacctg cagcatctgc accgataaca atagacatat tgtatactcc
    18301 tttttatttt ttctatgctg gattccagca gtgtattggc tgtctttaaa aaacagtcaa
    18361 ttagtcctca caaacataat atactactat tgtttgaaga agtcaatagt ttgtgtttgt
    18421 ttttgttatt tattttttac ttttttataa aacttctatc tcaaacccta attcttcaac
    18481 ctgattggct tctaagagac aaacattctt cttatcttct aaatgatctc cttcttcaag
    18541 gaatgttgac aagccagacc atggttcaaa ggtaatgaaa ggtcccttgt tcaaagttga
    18601 ccagatgatg aggtttggaa attctgcaaa atgcactttc aatcccttat catgtttaag
    18661 agaacgaagg gcaattgttc gagattgcaa ttcatccaaa gtcactgcat ctgtgctgaa
    18721 aagatcataa ctaagatcta tttctttttg tccctctagc catggacttc tatcttggaa
    18781 atccaacata cctgtttctg ggaaaggacg tggaacagag caagtctctt ctttctcaga
    18841 ctctagataa taatcttcat agactccatc atcaagtaga ggacaattaa atcctggatg
    18901 tccaccaata aagtaaggca tgaccttgct agtttcttta ttagacttcc tgcaaaacta
    18961 gaatcctagt tcatgattga taataccagc actcaaattc attcgtaatc cgaagcgttt
    19021 acgatgattt cgataggttg ttgaaaacat tttaaacgtt tttactttgg caaagatgtt
    19081 ctcaaccttg cttctctcct tagatagcgc atggttatag gctttatctt cagctattag
    19141 cgacttgagt ttgctggatt tacgtggagt ttgtacttga ggatatatct tcatgagtcc
    19201 ttgataacca ctgtcagcca agattttacc agcttgtccg atatttctgc aactcatttt
    19261 gaacaacttc atatcatgac aatagttcac agtgatatcc aaagaaacaa ttctcccttg
    19321 acttgtgaca atcgcttgag ccttcatagc gtggcatttc tttttaccag aatcattcgc
    19381 taattctttt tttaggtcga ttgatttgta cttccgtcgc atcaatcatt accgtgtcct
    19441 cagagctgag aggagttctt gaaaccgtaa caccactttg aacaagagtt acttcaaccc
    19501 attggctccg acggataaag ttgctttcgt gaataccaaa atcagccgca atttgttcat
    19561 aagttcgata ttctcgcact attgaagagt ggccataaga aggtcttcta ggcttaattt
    19621 aggttttcgt ccaccttttg cgtgtttaag ttgataagct gtttttaata cagctaacat
    19681 ctcttcaaaa gtggtacgct gaacaccaac aagacgctta aatcgtgcat cagttagttg
    19741 tttacttgct tcatcattca tagaactact ataccatatt ttgtttcgca ggaagtataa
    19801 tattgtcaaa tactggaacg ctcattgctg ggatacagaa taagattgga ccagcttcga
    19861 taactgggat acctggttca aaaccaaggt ctgttgcagc gattggtgta aagatatcgt
    19921 aacctttcat aaggtcttcg tttacatctt tcaccatgac tgcatcacag tgaacatcat
    19981 aaccacggtt tgaaagttct tcttctagag cacttttaat ttggtgactt gagttaacac
    20041 ctgcaccgca ggcagcaaga attttaatca tttggatttc ctccgatttt attttttaat
    20101 agacaagatt aagcggttgc ttcagcaatg taagcataaa gtttttctgg ttcagaaatt
    20161 tttgataggt cttcaagata
//
LOCUS       DAVKJJ010000037        19425 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000037 DAVKJJ010000000
VERSION     DAVKJJ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19425
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.037"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(71..3247)
                     /gene="carB"
                     /locus_tag="VW144_001412"
     CDS             complement(71..3247)
                     /gene="carB"
                     /locus_tag="VW144_001412"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU9600853.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(3559..4638)
                     /gene="carA"
                     /locus_tag="VW144_001413"
     CDS             complement(3559..4638)
                     /gene="carA"
                     /locus_tag="VW144_001413"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU9600854.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            complement(4688..5611)
                     /locus_tag="VW144_001414"
     CDS             complement(4688..5611)
                     /locus_tag="VW144_001414"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU9600855.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            complement(5630..6151)
                     /gene="pyrR"
                     /locus_tag="VW144_001415"
     CDS             complement(5630..6151)
                     /gene="pyrR"
                     /locus_tag="VW144_001415"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU9600856.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            complement(6362..6991)
                     /gene="nth"
                     /locus_tag="VW144_001416"
     CDS             complement(6362..6991)
                     /gene="nth"
                     /locus_tag="VW144_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU9600857.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            complement(6991..7533)
                     /locus_tag="VW144_001417"
     CDS             complement(6991..7533)
                     /locus_tag="VW144_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU9600858.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFISGNDWQIMTEEEYQAQKA
                     VKKEENSPFAGLQGLFDGDE"
     gene            7703..8020
                     /locus_tag="VW144_001418"
     CDS             7703..8020
                     /locus_tag="VW144_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU9600859.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EQLNNSILIDLEKFYQPTSLLIGLGSLKLNDQARTAWRNYDKFHYDHVKHVLSLYGPV
                     FEF"
     gene            8259..9818
                     /locus_tag="VW144_001419"
     CDS             8259..9818
                     /locus_tag="VW144_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9600860.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLTMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKVPEDLKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLQRLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRLLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(9862..10761)
                     /gene="htpX"
                     /locus_tag="VW144_001420"
     CDS             complement(9862..10761)
                     /gene="htpX"
                     /locus_tag="VW144_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEU9600861.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(10763..11323)
                     /locus_tag="VW144_001421"
     CDS             complement(10763..11323)
                     /locus_tag="VW144_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU9600862.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            11417..12130
                     /gene="rsmG"
                     /locus_tag="VW144_001422"
     CDS             11417..12130
                     /gene="rsmG"
                     /locus_tag="VW144_001422"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU9600863.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            12384..13667
                     /locus_tag="VW144_001423"
     CDS             12384..13667
                     /locus_tag="VW144_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEU9600864.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLIGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(13862..15433)
                     /gene="ffh"
                     /locus_tag="VW144_001424"
     CDS             complement(13862..15433)
                     /gene="ffh"
                     /locus_tag="VW144_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU9600865.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(15445..15777)
                     /locus_tag="VW144_001425"
     CDS             complement(15445..15777)
                     /locus_tag="VW144_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEU9600866.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            complement(15868..16245)
                     /locus_tag="VW144_001426"
     CDS             complement(15868..16245)
                     /locus_tag="VW144_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEU9600867.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            16317..17621
                     /locus_tag="VW144_001427"
     CDS             16317..17621
                     /locus_tag="VW144_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU9600868.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFEHLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQCNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            17634..18440
                     /gene="yidA"
                     /locus_tag="VW144_001428"
     CDS             17634..18440
                     /gene="yidA"
                     /locus_tag="VW144_001428"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEU9600869.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDEGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            18483..>19425
                     /locus_tag="VW144_001429"
     CDS             18483..>19425
                     /locus_tag="VW144_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HEU9600870.1"
                     /translation="MNFFSKLFNLKQNNHNRDTNSDCNNFYLNELECGLTPGQLILID
                     WTQKTGRNYNFPRYFKYSLQIDPESTHNQLYKLGYFTKNKTLSYLTVVELKTILSKHN
                     LATSGKKAELITRIINNVNIDNLDIPFEFKLTKEAQNLIIEHSDYIKAYYDKDITMED
                     YCKEKNNISFKATFGDIKWSLLNKQAHRNTVSGDFGCLSNTRKAQGRHLEQEGNIKHA
                     LIYYIESLIITISGLENNFSATDYPVYYPDSIPDYSLKHIQTLMESLSDDDYDFAFDE
                     ALFRFSILNANHFLSKEDIDYLRVNLPRSTAEEINNYL"
BASE COUNT         5734 a         4304 c         3696 g         5691 t
ORIGIN      
        1 aatttctaga aatattttag tatccctgtg tactgttcta aattcagttt gctatatctt
       61 atttttctat ttaaatcgct tctgtaacaa agctacgact ttcaagtacc ttaagcatag
      121 cattagctgt atctagcgct gtgaagaggg gcaccccgtg ttcaatggct gaacgacgaa
      181 tttgctcacc atcttcgtca gcagttcgtt ttgttcctac tgtgttaatg atagcttgaa
      241 ttcttccttt gcgtacaaaa cttgggatat ccttatcgtc atcaccaatc ttaccaacag
      301 gttgggcttg caagccatga ctagcaaaga aggctgctgt cccttctgtc gcaaggattc
      361 cataaccaat gttttggaaa cgacgagcca agttcaaggc ttcttctttg gcatcatcag
      421 cgatggtaaa gacgacatta ccaaaagttg gcaagtgtag ataagaagct tcaaaggctt
      481 tatagagagc tttttccaaa gtagcatcag aacccataac ttcacctgtt gacttcattt
      541 caggaccgag caagctgtct accttagcta gtttggtaaa ggagaagaca ggtgccttga
      601 tatgaacacg ggtgctttca gggtaaagtc cattttggta gccaagttct gataaacttt
      661 gaccaagaat gagcttggtc gctacttgag ccataggaat attggttacc ttagatagga
      721 atggaacggt acggctggca cgtggattga cctcaataac gtagactttt tcatccttga
      781 taacaaactg gatgttcatc attccaaggc agtgaagacc gattgctaag cgtttggtgt
      841 agtctgcgat ggtctcctga accttttgcg acaaggtttg tggtgggtaa acagccattg
      901 agtcacctga gtggacacca gcacgttcga tatgctccat gataccagga atgagtacat
      961 ttttaccatc tgaaatggca tcaacttcgc actcttgccc aacgatataa gagtcgacaa
     1021 gaactgggtg gtctggacta gccttaacag cagttcgcat gtaagaacga aggtcttctt
     1081 cgttttcaac gatttccatg gcacgtccac caagtacata agatgggcgg acaagaactg
     1141 ggaagccaat cttacgagct gcaagagctg cttcttcttc attggtagcc gtttgtcctg
     1201 gtggctgtgg aatatccaat tctttgagag cttgctcgaa gaggtcacgg tcttcggcac
     1261 ggtctaggtc agcaacctgt gtaccaagga tggtcacacc tgcttttgcc aatggctccg
     1321 caaggttgat ggctgtttga ccaccgaact gaacgataac tccctttggt tgttccaagt
     1381 caatgacgtt cataacatct tcgaatgtca atggctcaaa gtaaagctta tctgatacag
     1441 agaagtctgt tgaaacggtc tctgggtttg agttcatgat gatagcttca taaccagctg
     1501 cctggatagc cttaacagag tgaacggttg cgtagtcaaa ctcaacccct tgaccgatac
     1561 ggattggacc tgaacctagg acaagtacag attctttatc agatctgata gattcatttt
     1621 cccaaccata ggttgaatag aaatatggcg tttcggagtc gaactctgcc gcacaagtgt
     1681 ctaccatctt ataaactgga acaatcttgt tttccaagcg aagttggcga actttatcat
     1741 cagtcgttcc ccagagttca gcaatcttac ggtctgaaaa accattaagt ttggctgttt
     1801 tcaaaacttc tagatcttgt ggatgagcac ccaattcttg ctcaatttca aagatatgca
     1861 agagtttatc aagatagaag atatcaattt ttgtaagctc tgcaatttct tcaggtgtgt
     1921 agccacgacg aattgcttct gatacgtaga agagacggtc atcttgggct ttgacaacct
     1981 tttcaatcaa ggcatcatca gaaactgctg caagttcagg tatttcattg tggtgcaccc
     2041 caatttcaag ggatcggcag gccttgagaa gagattcctc gatgttacga ccgattgcca
     2101 tgacttctcc agtcgccttc atttgtgtac cgagacggcg ttcacccttt tcaaacttgt
     2161 caaatgggaa acgtggaatc ttagcaacta cgtagtcaag ggctggttca aacatggcat
     2221 aggttgaacc tgtaactggg tttataacct catccaaggt caaacctact gcaatcttgg
     2281 cagccaactt agcaatcgga tatcctgtcg ctttagaagc aagggctgac gaacgtgata
     2341 cacgagggtt tacttcgata acataatact tgaagctgtt aggatcaaga gctagctgaa
     2401 cattacatcc accttcaatc ttgagggcgc gaataatgct caagctcgca tcacgaagca
     2461 tttggttttc atagtctgac atggtttgcg caggggcaaa tacaatggaa tcccctgtgt
     2521 gaatcccaac tgggtcaaag ttttccatgt tacaaacaac caaggcattg tcagctgagt
     2581 cacgcatcac ttcgtattca atttccttga aaccggcaat cgaacgctca atcaaacatt
     2641 gggtaacagg tgacaatttc aaaccatttt cagtgatttc acgcaattct ttttcgttgg
     2701 cacacatacc accaccagta ccaccaaggg taaaggctgg acgaacgatg actgggtagc
     2761 caattgtcgc tgcaaaggca actgcttctt ctactgtgtt aacaatttca gattctggaa
     2821 tgggttgctc aagctcttcc atcaattgtt taaagaggtc acggtcctcc gcttggtcaa
     2881 tggcagataa tttggtaccc agaagttcaa cgccaagctc gtctaggata ccatttttag
     2941 ataattccat ggccatgttg agacctgtct gaccaccgag tgttggtagc aaggcatctg
     3001 gaccttcctt acgaagaata cgtgtcacaa actcaagtgt aatcggttca atgtaaacct
     3061 tgtcagcaat ttccttgtcc gtcatgatgg ttgcaggatt tgagttaacc aaaacaacct
     3121 cataaccttc ctctttcaac gacaagcaag cctgagtccc agcgtagtca aactcagcag
     3181 cctgaccaat aataatcgga ccagaaccaa tcaccataat tttttgaata tcagtacgtt
     3241 taggcatagt ttatgataca aagcccgtaa agaacacagt gaaaatagga aactcggcgc
     3301 agacgccttc agcgtcaaga cgatgtttat ctttttcaca cagttcttag ggcgtgttca
     3361 cttccgcgaa caatgtatct tctcctttct attcgtcgcc tctcagggcg acattaaata
     3421 agatacaaag gacgaataga aagcgattga attttaggaa atcaaggaag gattgacaat
     3481 ccaagttggt ttctctacat tctgagcttt ccgtccgtgt tcagttacat aaattctccg
     3541 acgagctttt actcgttctt agtttgattg tttaaaaact tccatcatct cgataaactc
     3601 gtcaaatagg tagctagcgt cgtgtggccc aggagctgca tctgggtggt attgaacaga
     3661 gaaagcaggt tggtatctgt ggcgcacacc ttccactgac ttgtcattga tttcttcgtg
     3721 ggtaataatc aagtgctctg gcaaatcctc gcggctgact gcataaccat ggttctggct
     3781 ggtgaagtct actcgtcctg ttgcgatttc acgtaccgca tggttgaatc cacggtggcc
     3841 aaacttcatc ttataggtct tagccccgtt tgccattgca aagagttggt gtcccataca
     3901 aataccaaag attggaattt ttccttgtac accgcgaatc atgtcgagtg cttgtggaac
     3961 gtcttctggg ttacctggac catttgacaa cataactccg tcaggattga gatggagaat
     4021 ttcttcagcc gttgtcgaat aaggaacaac tgtcacgtta cagttgcgtt tagaaagttc
     4081 acgtaggatt gagtgcttga gaccaaagtc cactagcacc acgctcaaac caactcctgg
     4141 agctggataa gacgttttag tagaaacctg tttgatattg tctgtcggta aaactgttgc
     4201 ttggagctgg tccgtcacat ggtccatact gtccccaaca tgggtcaagg ttgcacgcat
     4261 agtaccatgc ttacggataa tcttggtaag agcacgcgta tcaattcctg aaatccctgg
     4321 aattttcttg gctttcaaaa attcatccaa ggtcatttgg ttgcgccagt tgctagctct
     4381 acgcgcttct tcaaaaacaa cgactccctt acaagttgga ataatggatt cataatcatc
     4441 acgattaata ccataatttc ctaccaaagg ataagtaaag gtcaagattt gtccattata
     4501 agactggtct gtaatggatt cttggtagcc ggtcatccct gtattaaaga cgatttcgcc
     4561 tgttacatca atatctgctc cgaaggcctt gccttcaaaa actgtgccat cttctaatac
     4621 tagaattctt tttgtcatat tttcacctct cgtggacgct cactggcgtc ttttaacgtc
     4681 ttgtgtttta gttggcgttt ctactcgcta gtacggattc taagattgcc attcgaacaa
     4741 agacaccatt ggtcatttgt tggacaatcc gtgattttgg tgcttcaacc aagtggtctg
     4801 ctatttctac atcacgattg attggagctg ggtgcatgag gattgctgtt tctttcaaac
     4861 gatcgtaacg ttcttgagtc aagccatgtt gggcatggta gtcttctttt gaaaatacag
     4921 ctccactatc atggcgttcg tgttgcacac ggagaaacat catgacatca acctgatcaa
     4981 tgatttcatc aatggttaca aactgtccat agtctgcaaa ctcttgactt ctccattcct
     5041 caggtccagc gaaaaagagt tcagatccca agcgtttcaa aatctgcata ttggatttgg
     5101 caacgcgtga gtggtccaag tcacctgcaa tagcaacttt aagaccctca aagtggccaa
     5161 attcctcata aatggtcatc aaatcaagca agctctggct agggtgttgg cccgaaccat
     5221 ctccaccatt gatgatggaa gtcgtaatcg ttggactagc aatcaattct ctatagtagt
     5281 cgacctctgg atggcgaatc acacagacat ccactcctaa agcagacaga gtcaaaatgg
     5341 tgtcataaag tgtctcaccc ttattaaccg agctagtctt cacatcaaag tcaagtcgtt
     5401 ccaatccaag tttaatctct gcgacttcaa aggacttatg tgtccgtgta gaatcctcaa
     5461 agaagagatt ggaaacaatc ggatggtctt catagggaag ctgggctcca tttttaaact
     5521 caattcctcg cttgatcaat ttcattactt gatcgacagt gaggtcttcc atggacacca
     5581 catggttcaa tgcttgttga ttttctgaca tggctactcc tttaactttc taagcttctt
     5641 cagtaatcag aactctgtct tggtcatcaa gttctgtcat ctctacgatg atttcttcag
     5701 aacgactggt tgggatattt tttccaacgt aatctggacg gattggcaat tctctatgtc
     5761 cacgatcgac tagaactgct aaactcacac gcgcaggacg accatgaccg acaatattat
     5821 caatagcagc acggatggta cgacctgtat agagcacatc atccaccaag ataacttcgc
     5881 ggtctgtcac atcgacagaa accaaagaag tatcttctcc acttttaaca tcatcacgga
     5941 aaggtttagt atccaattcc acaacaggaa ctgaaagatt ttctaactgc ttcaaacgtt
     6001 cttggattcg gtgggcaata aagacaccac gagttttaat accagccaag acgatcttat
     6061 tcaaatcttt gttgcgttcg ataatctcat aagtaatacg cgtaatcgct cgtttgacgg
     6121 tcaattcgtc tacaacttct tttgttttca tgacaaacct ccaaaaagaa aagtctcctt
     6181 aaacaaggag acttgaaatt tatagccaag cgagccctac tgcacacagt atagacttca
     6241 cccttctact ttatcgcgct ccttgcctgc ctcacgggac aggtttaaag gaatatttag
     6301 ttatcattta ctatagcaca aagcatgctt aaaatcaagc aaaaagtttc aatgtagcat
     6361 cttacaaatt gctaaaatca tataattgtg ggtactggtc acactctgga ttttttggat
     6421 ggcaaatggc tcttccaaaa taaatcatgg cctgatgggc agctaaccac tgctcaggcg
     6481 gcaagatatc catgacccgc ttttccacct caagtggcgt cgctgatttt ttgacaatat
     6541 cgtggtgttt gcaaatacgc tccacatgag tatccactgc aaaggctgga attccaaatc
     6601 ctacactcat gacaacattg gctgtcttgc gaccaacacc tgccaaactc tccaattctt
     6661 cacgtgtctg agggacttga ccatcaaaat cgtctagtaa ctgttgggca cattttttaa
     6721 ggaatttagc tttattccga tacaatccca agcgagaaat atgtgaagca atctcactct
     6781 ctgtcgctac agacatagct tggggtgttg gaaaggcaac aaagagacct ggtgtggcct
     6841 tatttaccgc tgcatctgtc gtctgggctg ataacatgac cgcaaccagg agttcaaaat
     6901 gattggtaaa atcaagacta ggcttggcat ctgggaagag ggcaatgatt tcttctagca
     6961 cctttcgtgc tcgttttttt gacaagacca ttattcatct ccgtcaaata gtccttgtaa
     7021 gccagcaaaa ggactgtttt cttctttctt tactgctttt tgagcttggt attcttcctc
     7081 tgtcatgatt tgccagtcat ttcctgagat aaatccttga ccagcttctt cttcagccgt
     7141 caagaccttg ataggaatgt ttagcaggat attgtctgat acactctcag caaggtcaag
     7201 ctccccattt tcgatgggca agaccaagtc atcatctaaa acttcttgat ctagctggtt
     7261 agttgcgcct tccatgaaaa cttccgtgac tggataagat tcaactaact caactggctc
     7321 catactgcga ctcgaagcaa gaacaatggt ataagatagt tgataatcta agaaatacat
     7381 acggtcttca tattgtactt tcccaactgc aaggatatct tttacatcta aaatttcttg
     7441 attacgtgca cgcaggtcat caactaaatc taacgtttgt tcaaagttca aaccttcaga
     7501 ctgcttacga atttcttgaa tatttaattt catacttcct ccataaagat ttactctctt
     7561 gattatacca tgaaaaggct acaaatcagc acaccaaact ttgtaattaa aattcaaaat
     7621 tttaacatat ttactatgat agttttattt tttagtgcta tactataggg aaagagtaca
     7681 tcagatcaag gaggatgctc acatggaaga caagaaactc attcaactcc tatccaagtt
     7741 aaataaaagc taccaaaact gtaaacaggg tacggcagat gatattcgac tacaagagct
     7801 gctaaacact actatgcaag agctcaaaaa aacggaacag ttgaacaaca gtatcttaat
     7861 tgatcttgag aaattttacc aacctaccag tcttctgatt ggactgggta gcctaaaact
     7921 aaacgatcaa gcacgcactg cttggcgaaa ctatgataaa ttccattacg atcatgtcaa
     7981 acacgtacta agtctctatg gacctgtttt tgaattttag agcatagaat ttccagtttt
     8041 ctgttgacaa aatttcctta aaggtataat ataaagatac taatactcgg aggtaaggga
     8101 gacatgaaca actaagtcta tcaaataaag aacctttatt tagtagatct tgtttttgtc
     8161 tctttttgtg tgctctttta tgctcttttt ctggcatgtt aatagagttt ttttgacata
     8221 gactttgggc tctactaggt aaagtagagc tttttgttat gcactatgaa cattctagaa
     8281 agggaaatca tatgataaaa atcaatcatc taaccatcac acaaaacaaa gatttacgag
     8341 atcttgtatc tgacctaacc atgaccatcc aagacgggga aaaggttgct attattggtg
     8401 aagaaggaaa tggcaaatca accttactta aaattttaat gggggaagct ttgtctgatt
     8461 tcactatcaa gggaaacatc caatctgact atcagtcact ggcctacatt cctcaaaaag
     8521 tccctgagga cctaaaaaag aaaactttac acgactactt ctttttagat tctattgatt
     8581 tagactacag tatcctctat cgtttggcgg aggaattgca ttttgatagc aatcgtttcg
     8641 caagtgacca agagattggc aatctatcag ggggcgaagc tttgaaaatt cagcttatcc
     8701 atgagttagc caaacccttt gagattctat ttttagatga accttcaaat gacctagacc
     8761 ttgagacagt tgattggcta aaaggccaga ttcaaaagac caggcaaacc gttattttca
     8821 tttcccatga tgaagacttt ctttctgaaa cggcagacac tattgttcac ttgcgactgg
     8881 tcaaacaccg taaagaagcg gaaacgctag tagagcattt agactatgat agctatagtg
     8941 agcagagaaa ggctaatttt gccaaacaaa gtcagcaagc tgctaacaac caaagagcct
     9001 acgataaaac catggaaaaa catcggagag ttaagcaaaa tgtagaaact gcgcttcgag
     9061 ctaccaaaga tagtactgcc ggtcgcctat tggctaaaaa gatgaaaact gtcctctcac
     9121 aagaaaaacg ctacgaaaag gcagctcagt ccatgactca aaagccactt gaagaggaac
     9181 aaatccaact tttcttttca gacatccaac cattaccagc ttctaaagtc ttagtccaac
     9241 tggaaaaaga aaatttgtcc attgacgacc gagttttggt tcaaaaacta caactaactg
     9301 tccgtggcca agaaaaaatc ggtattatcg ggccaaatgg tgttgggaaa tcaactctgt
     9361 tagccaagtt acagagactt ctgaatgata aaagagagat ttcacttggt tttatgccac
     9421 aagattacca caaaaaactg caattggatt tatccccaat agcctatctc agtaaaactg
     9481 gggaaaaaga ggaactacag aaaatccaat ctcacctagc tagtctcaat ttcagttatc
     9541 cagaaatgca gcatcaaatt cgctccttat ctggcggaca acagggaaaa ctcctgcttt
     9601 tggatttagt cctgcgcaaa ccaaactttc tcctgctgga tgaacccaca cgaaactttt
     9661 ctcccacttc tcaaccccaa atcagaaaac tctttgctac ctatccaggc ggtctcatca
     9721 ctgtttcgca tgaccgtcgt ttattaaaag aagtctgctc gatcatctat cgcatgacag
     9781 aacacggttt gaagctagtt aatttagaag atttataaat ttgcaacata gcaaaaatcc
     9841 agagacgacc tctggattct tttacatctg ttttaaacgt tcaatccgtt ctgagatagg
     9901 tgggtgggta taaaagagtt tttggaaccc cccacctttc ttaggatcat tgatataaag
     9961 ggcactgcta gcatcatcga cgtggcgact cataggtttg ctattgtcca acttatctag
    10021 ggcattaatc attccctggg gattgcgagt cagctcgaca ctagatgcat ctgccagaaa
    10081 ttccctctga cgagaaatag cgagctgaac caaggttgca gcgagaggtg ccagtacaat
    10141 agctagtagg gaaaccacta gcataatgat ttcaagacca tttccatctc ggtcatcatc
    10201 acttcgtctg cgacctgctc caccccacca catcatacga cctgccatac tagaaagcat
    10261 ggtgatagca ctagcaaggg caactgcaat agtcgaaata cggatatcat aattacgaat
    10321 atgactgact tcatgtccca taacagcttc tagttcttca cgattcatga tagctagtag
    10381 acctgaagtc gcagcaacag ccgcattttg aggattagaa cctgtcgcaa aggcatttaa
    10441 ggctggatca tcaatgatga aaacacgggg cataggaatc tgagcgacca gagccatatc
    10501 ttccactaca tggtagaggt ctggtgccgt ttgctcatcc acctcacgcg ctccattcat
    10561 ggacatgaca atctctgtcg attgaaaaat catagacaaa gcgtagataa agccgataat
    10621 cagtgcaata accaaaccac caagtccaga tcttataaag agataaccaa ccgcataacc
    10681 aacaagagct aagagtagga aaaataccag caacaaaatc caggtttttc gtttattgct
    10741 tgcaatttga tcaaacaaca tcttagtcac ctaaaccgct aaaatcaact ttaggaaccg
    10801 acttttcctc ttcaggtgtt tgaaggaaat ctgccgcttt aaatccaaac attccagcga
    10861 taatattgct cgggaaagtt tctaatttta cattgtagtt gctgacaaca ctgttataga
    10921 gttgacgaga gtaagaaatt ttattttctg tgtttgtcaa ctcctcttgc aatttaacaa
    10981 agttagcact agctttcaaa tctggatagc tttctgcaac tgcaaaaata cctgaaacct
    11041 gacgagtgag ggcatcactg gctttcatag cttctgctgg tgaagtcgct gccgccactt
    11101 ggttacgtag ttctgccacc ttttcaaggg tagaaccttc atatttggca taacctttta
    11161 cagtctcaat caagtttggc aagaggtcat tgcgacgttt caactgaaca tcaatctgac
    11221 tccaagcctc cttggtttgc atacgatttt taaccaaacc gttatagcta acaatcacaa
    11281 aaataacaat aagagcgata actccaagaa taatccaagt cataatataa gtcctttctg
    11341 cttttagatt agtaccagta tatcaaattt tctatgattg tggtaaaata agatgatact
    11401 aaagaaggaa ataactatga aaccaaaaac attttacaac ttgcttgccg agcagaatct
    11461 tccactttcg gaccagcaaa aagaacaatt tgaacgttat tttgagctct tggtcgagtg
    11521 gaatgagaag attaatttga cggcgattac ggacaaggaa gaagtttatc tcaaacattt
    11581 ttacgattcg attgcaccca ttcttcaagg tttgattccc aatgaaacta tcaaacttct
    11641 tgatatcggg gctggggcag gatttcctag tctaccaatg aaaattctct atccggagtt
    11701 agatgtgacc attattgatt cactcaataa gcgcatcaac ttcctacaac tcttggctca
    11761 agaactggat ttgaacggag ttcatttcta ccacggacgt gccgaagatt ttgcccaaga
    11821 caagaacttc cgtgctcaat atgattttgt aacagctcgt gcggttgccc gtatgcaggt
    11881 cctatctgaa ttgactattc cctaccttaa ggttggtggc aaactattag cactcaaggc
    11941 tagcaatgcg cctgaggaat tattagaagc taagaatgcc ctcaatctcc tttttagtaa
    12001 ggtcgaagac aatctcagct acgccctacc gaatagagat ccgcgctata tcacagtggt
    12061 agaaaagaaa aaagaaacac caaataaata tccacgtaag gctggtatgc caaataaacg
    12121 cccactttaa attttttagt aaacaaatgt ttacaaaatc agcctcgctc ttttatttct
    12181 aggctcggga aaaaatgatt tacaaaatca tttttttctg ctatactatc ctaagcaaag
    12241 gtttttaatg tcatcccgtg aggtgacgaa gacgcagaaa tatttaaaac tctttaaaat
    12301 ctaaatttta aagaagtctt actctgaggg cctattgctg taaaataatg ggctcttttt
    12361 tgatgcccaa aagtgaggtt tatatgaaac aagaatcaac tgttgatttg ttactagacg
    12421 ttgaccaacg tccttcggct ggaaaaggaa ttctccttag tttccaacac gttttcgcca
    12481 tgtttggtgc gaccatcttg gtaccattga ttttgggaat gcctgtatct gttgcccttt
    12541 ttgcttcagg tgttggaaca ctcatctaca tgattgctac tggttttaaa gttccagttt
    12601 atctaggttc ttcattcgcc tttatcacag ctatgtcact ggctatgaaa gaaatggggg
    12661 gggatgtatc tgctgctcaa acaggggtta tcttgactgg tttggtctat gtccttgttg
    12721 ctaccagcat ccgatttgta ggaacaaaat ggattgataa actcttgcca ccaatcatta
    12781 tcggtcctat gatcatcgtt atcggtcttg gacttgcagg ttcagctgtt accaatgcag
    12841 gtcttgtagc agacggaaat tggaaaaatg ctctggtagc cgttgttact ttcctaattg
    12901 ctgcctttat caatacaaaa ggaaaaggct tcctacgaat cattccattc ctctttgcca
    12961 ttatcggtgg ttacctcttc gcgctaactc ttggtttggt tgactttacg cctgtcctag
    13021 aagctaactg gtttgaaatt cctggtttct acttgccatt tagcacaggt ggtgccttta
    13081 aagagtacaa tctttacttt ggtccagaag ccatcgctat cttgccaatc gctatcgtaa
    13141 caatttctga acatatcgga gaccatactg ttttgggtca aatctgtggc cgtcaattct
    13201 taaaagaacc aggtcttcac cgtactctta ttggtgacgg tatcgcaact tctgtttctg
    13261 ccttccttgg tggaccagcc aatacaactt acggagaaaa tacaggggtt atcggtatga
    13321 ctcgtatcgc ttctgtctca gttatccgta acgctgcctt catcgcgatt gccctcagct
    13381 tccttggtaa attcactgcc ttgatttcaa ctattccaaa cgctgtactt ggtggtatgt
    13441 caatccttct ctatggggtt atcgccagca atggtttgaa agtcttgatt aaagaacgtg
    13501 ttgatttcgc tcaaatgcga aacctcatca tcgcaagtgc tatgttggtt cttggacttg
    13561 gaggagctat ccttaaactt ggtccagtta cactttcagg tactgccctt tcagccatga
    13621 caggaatcat cttgaacttg atcttgccat acgaaaataa agactaagag tctaaataca
    13681 cctaatccac tcagacagct gagtggattt ttcgtatact ataataaaag tgtcttaaca
    13741 aaattattaa aatcaaaaaa cgtataatat cagatattct aaaaccttga tactgtacgt
    13801 tttatcatag aaatttttac tttattttct catcaaatga gatttgcatc aatctcttgt
    13861 cttacttgcg tttcttcttc gctttcttca ttttgttagc catacgtttc atggactgtt
    13921 tcatggcaaa ttcaccaatt ttacctttca aaccgccacc aaacatctgg ctcatatctg
    13981 gcattcctgc tcctccgaga gctgataagt caggcatacc gccttgtccc atcattcctt
    14041 caagggcaga catatccatt cctcccatat ttggcatatt tttaggaagg ttatttggat
    14101 taatccccat ttgcttcatc attttattca tatccccaga cataacaccc tgcatgagct
    14161 gtttagcctg gttaaagtcc ttgatgaatt tattgacttc gacgaatgta tttccagaac
    14221 cagcagcaat acgacggcga cggcttggat ttaacaaatc tggattttca cgttcttcag
    14281 gtgtcatcga agacacaatc gcacgtttac gagcaatctg gcgttcatcc accttcatgt
    14341 tttgaagtgc tggattgttg gccatacctg gaatcatctt gagcaagtct tccatcggcc
    14401 ccatattttg cacctgatct aattgatcga tgaaatcatt aaaatcaaag gtgttttcgc
    14461 gcatcttctc agccatttca agggcttttt gttcatcgta ttcctgagaa gctttctcaa
    14521 tcaaagtgag catatccccc atgccaagga tacggctaga catgcggtct gggtggaagg
    14581 tttcaatgtc cgtaatcttt tcacctgtac cagtgaactt gattggtttt ccagtaatgt
    14641 gacgaacaga cagagcagca ccaccacgag tatcgccatc aatcttggta aggatgaccc
    14701 cagtcacttc caactgagca ttaaactcac gcgcaacatt ggctgcttcc tgaccaatca
    14761 tagcatcaac gacaagcaag atttcatttg gttgagccaa tgctttcaca tcacgaagct
    14821 cattcatgag gagctcatca atctgcaaac gacccgcagt atcaatcaag acatagtcgt
    14881 tatgattagt ttgagcttgc tccaaacctt gacgtacaat ctcaacagct ggaacctctg
    14941 tcccaagagc gaaaaccggc acatcaatct gttgtcccag tgtcttcagc tgatcaatgg
    15001 cagcaggacg ataaatatcc gccgcaatca tcaaaggacg agcattttct tctttcttga
    15061 gtttgttggc caatttacca gcaaaggttg ttttaccagc cccttgtaaa ccaaccatca
    15121 tgatgatggt tggaatctta ggtgacttga taatttctgc cgtatcagaa cctaaaacgg
    15181 ctgtcagttc ctcatcaacg attttaataa tctgttgcgc aggattaagt gtatcaatga
    15241 cctcatgccc gactgcacgc tcacgaactt tcttgataaa gtcctttaca acaggcaagg
    15301 caacgtcggc ctcgagcaag gccaagcgaa tttctttggt tgcctcttgg acatcagatt
    15361 cagagatttt tcctttttta cgtagatttt taaagacgtt ctgcaaacgt tctgttaaac
    15421 tttcaaatgc catttttctt cctcttattc tctattatca atgcttgtta aaatttctat
    15481 ctgctcctgc agaaagtcat ccttgggata gcgctccaaa atctgatcaa aaatctgact
    15541 gcggacaata tagtccgagt acatgtgcaa tttcatctca taatcttcca gaatcttttc
    15601 tgttcgcttg atattgtcat agacagcctg acgactgaca ccgaactcct cggcaatttc
    15661 agcaaggctg taatcatcag cgtagtagag ctcgatataa ttcatttgct tatctgtcaa
    15721 aagcgccgca taaaattcaa agagcgcatt catacgattg gttttttcga tttccataac
    15781 ttttattata ccaaaaatta gcctaatcta ccacactagg aagccgatcc aagaagatag
    15841 atagctaaat ttgaaaaaga catgagccta gccccaagta atttccaatt gatagctggc
    15901 aaagggatgt ccctcttgat tttgtagttg ataatctagt tcaatctttt gcctatcaac
    15961 ttgataatgg ctcgtttgga tgataaactc ctgcatgccc ataggtgtag gaatataggc
    16021 taaactatcg ctatccttta gaaagcgcat aatggtcttg ggattagaaa atcggctcat
    16081 cacaagttct tgaccatgaa atttaatcac tactttttcc ttttcctcat tatagaaaag
    16141 caggtagcta taatctcctt tttcatgcac ttccacatca taaagctggt caatcacttc
    16201 caactgctca tcaaactgaa tcgtatttcg catccgaatc ttcacatcag gccctctttc
    16261 ttgtctcttg tcctactatt ttaccaaaaa gagcaggatt ttgctataat ggtcatatga
    16321 acgaaaaagt attccgtgac cctgttcaca actacatcca tgtcaataat caaatcatct
    16381 atgacttgat taatacaaaa gaatttcagc gtttgcgccg gatcaaacaa ctgggaactt
    16441 ccagttatac cttccacggt ggagaacaca gtcgcttctc tcactgtcta ggagtctatg
    16501 aaattgcacg acgcatcaca gagattttcg aagaaaaata tcctgaggaa tggaatcctg
    16561 ccgagtctct cttgaccatg accgctgctc tcctacacga ccttgggcat ggtgcctact
    16621 cccatacttt tgaacatctc tttgatacag accatgaagc cattactcag gagattattc
    16681 aaaatcctga gacagagatt caccaagtcc tgctacaagt ggcacctgat ttcccagaaa
    16741 aggtggccag tgtcattgac catacctatc ctaataagca ggtcgtgcag ctcatttcta
    16801 gtcagattga cgcagatcgc atggactatc tcttgcgcga ctcctatttt acaggagcat
    16861 cctatgggga atttgacctg actcgaatcc tccgagtcat tcgtcctatc gaaaatggta
    16921 tcgcctttca gtgcaatggc atgcacgcca tcgaagacta cgtcctcagt cgctaccaga
    16981 tgtacatgca ggtttatttc caccccgcaa cacgcgccat ggaagttctc ctacagaatc
    17041 ttctcaaacg cgccaaggaa ctctatcctg aggacaagga tttctttgcc cgaacttctc
    17101 cacacctcct gcctttcttc gaaaaaaatg tgaccttgac tgactatctg gctctggatg
    17161 atggcgtgat gaatacctac ttccagcttt ggatgaccag tcctgacaag attcttgcag
    17221 atttatcgca tcgctttgtc aaccgcaagg tctttaaatc cattaccttt tcacaagagg
    17281 accaagatca acttactagc atgagaaaat tggttgagga tatcggcttt gatcccgact
    17341 actacactgc cattcataag aactttgacc tcccttatga tatctatcgt cccgaatctg
    17401 aaaacccacg gacacagatt gagattttac aaaaaaatgg agaactggcc gaactctcta
    17461 gcctgtctcc tatcgtccaa tcccttgctg gcagtcgcca cggagataat cgcttttatt
    17521 ttccaaaaga aatgttggac caaaacagca tctttgcaag cattacccag caatttttac
    17581 acttgattga gaacgatcat tttaccccaa ataaaaacta gaagaggaaa tttatgagta
    17641 ttaaactaat tgccgttgat atcgacggaa cccttgtcaa cagccaaaag gaaatcactc
    17701 ctgaagtttt ttctgccatc caagatgcca aagaagctgg tgtcaaagtc gtgattgcaa
    17761 ctggccgccc tatcgcaggc gttgccaaac ttctagacga cttgcagttg agagacgagg
    17821 gggactatgt ggtaaccttc aacggtgccc ttgtccaaga aactgctaca ggacatgaga
    17881 ttatcagcga atccttgact tatgaggatt atctagatat ggaattcctc agtcgcaagc
    17941 tcggtgtcca catgcatgcc attaccaagg acggtatcta tactgcaaat cgcaatatcg
    18001 gaaaatacac tgtacacgaa tcaaccctcg tcagcatgcc tatcttctac cgtacccctg
    18061 aagaaatggc tggcaaagaa attgttaaat gtatgtttat cgatgaacca gaaattctcg
    18121 atgctgcgat tgaaaaaatt ccagcagaat tttacgagcg ctactccatc aacaaatctg
    18181 ctcctttcta cctcgaactc cttaaaaaga atgtagacaa gggttcagcc attactcact
    18241 tggctgaaaa actcggattg accaaagatg aaaccatggc aatcggtgat gaagaaaatg
    18301 accgtgccat gctggaagtc gttggaaacc ccgttgtcat ggaaaatgga aatccagaaa
    18361 tcaaaaaaat cgccaaatac atcaccaaaa caaatgacga atccggcgtt gcccatgcca
    18421 tccgaacatg ggtactgtaa aagtatcatt tttcaataag aattgattag caataaaatc
    18481 caatgaattt ttttagcaaa ctatttaatt taaaacaaaa taatcataat agagacacaa
    18541 attctgattg taacaatttt tacctaaacg aattagaatg tggccttact cctgggcaac
    18601 tcatactcat agattggact caaaaaacag ggagaaatta taatttccca agatatttta
    18661 aatactctct tcaaattgac cctgaatcta cacacaatca attatacaaa ttaggatact
    18721 tcactaaaaa taagacttta tcatatctta cagtagtaga attaaaaact atattatcta
    18781 aacataattt agctacttct ggaaaaaaag cagaattaat tacaagaata attaataatg
    18841 ttaacattga caatttagat attccgttcg aatttaaact aacaaaagaa gcacaaaatc
    18901 ttattatcga acatagtgac tatatcaaag catactatga taaagacata actatggaag
    18961 attattgtaa agaaaaaaac aatatctctt ttaaagcaac ttttggtgat ataaaatgga
    19021 gtctcttaaa taaacaagct cataggaata ctgtatcagg agattttgga tgcttatcta
    19081 acacacgaaa ggctcaggga agacatttgg aacaagaagg taatattaaa catgctttaa
    19141 tatattacat agaatctttg ataattacta tttcaggatt agaaaacaat ttttcagcca
    19201 ctgattatcc agtatattat cccgattcga tacctgacta ctcactaaaa catattcaaa
    19261 cattaatgga atcattatct gatgacgatt atgattttgc ttttgatgaa gcattatttc
    19321 gcttctcaat tttgaatgca aatcattttt tatctaagga agatattgac tatttaagag
    19381 ttaatttacc tcgttccact gctgaagaaa taaacaatta cttaa
//
LOCUS       DAVKJJ010000038        18231 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000038 DAVKJJ010000000
VERSION     DAVKJJ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18231
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.038"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            156..1637
                     /locus_tag="VW144_001430"
     CDS             156..1637
                     /locus_tag="VW144_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9600871.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            1998..2999
                     /locus_tag="VW144_001431"
     CDS             1998..2999
                     /locus_tag="VW144_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9600872.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            3150..4067
                     /locus_tag="VW144_001432"
     CDS             3150..4067
                     /locus_tag="VW144_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9600873.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYDYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            4078..4968
                     /locus_tag="VW144_001433"
     CDS             4078..4968
                     /locus_tag="VW144_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9600874.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            4997..6613
                     /locus_tag="VW144_001434"
     CDS             4997..6613
                     /locus_tag="VW144_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9600875.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKATDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTIPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSNYLAIKQKYYDQYQANKN"
     gene            6623..7942
                     /locus_tag="VW144_001435"
     CDS             6623..7942
                     /locus_tag="VW144_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9600876.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKTGVRQVQNMAFSDDGIHFEKISQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWREKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(8150..8222)
                     /locus_tag="VW144_001436"
     tRNA            complement(8150..8222)
                     /locus_tag="VW144_001436"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8187..8189),aa:Lys,seq:ctt)
     gene            complement(8487..9758)
                     /locus_tag="VW144_001437"
     CDS             complement(8487..9758)
                     /locus_tag="VW144_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU9600877.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWIKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            10056..10271
                     /locus_tag="VW144_001438"
     CDS             10056..10271
                     /locus_tag="VW144_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600878.1"
                     /translation="MMELPSQEILIFTKQIRHWILSDQVISGKRKLFFREDTPKEILD
                     LYENIKSKLDFAYQEVHSNNGLKKYEK"
     gene            10436..10615
                     /locus_tag="VW144_001439"
     CDS             10436..10615
                     /locus_tag="VW144_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600879.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSVPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            10752..10931
                     /locus_tag="VW144_001440"
     CDS             10752..10931
                     /locus_tag="VW144_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEU9600880.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGE
                     LNKYTERGIRKQAGL"
     gene            10968..11420
                     /locus_tag="VW144_001441"
     CDS             10968..11420
                     /locus_tag="VW144_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU9600881.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            11711..12181
                     /locus_tag="VW144_001442"
     CDS             11711..12181
                     /locus_tag="VW144_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEU9600882.1"
                     /translation="MATYGFLDILEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKIGLEKGEFYPYPKY"
     gene            12200..13288
                     /locus_tag="VW144_001443"
     CDS             12200..13288
                     /locus_tag="VW144_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU9600883.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAILFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENWLAELEKEMLEGTEDGTRNF"
     gene            13269..13697
                     /locus_tag="VW144_001444"
     CDS             13269..13697
                     /locus_tag="VW144_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9600884.1"
                     /translation="MELEISDFTGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     REGDESPFECVAREVYEELGIHLTEDCLLWSKVYPSMLFADKQSVFLVGQLTQNQFDS
                     IVFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            13833..14783
                     /locus_tag="VW144_001445"
     CDS             13833..14783
                     /locus_tag="VW144_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU9600885.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARLAELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGILSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDKW
                     DMVRELAESAGFFLETHMVQGEWNACVFKKTKDISGVIGG"
     gene            14785..15528
                     /locus_tag="VW144_001446"
     CDS             14785..15528
                     /locus_tag="VW144_001446"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU9600886.1"
                     /translation="MQQYFVKGSAISPVTIENKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            15539..17335
                     /gene="pepF"
                     /locus_tag="VW144_001447"
     CDS             15539..17335
                     /gene="pepF"
                     /locus_tag="VW144_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9600887.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFENFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKHSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            complement(17395..18072)
                     /locus_tag="VW144_001448"
     CDS             complement(17395..18072)
                     /locus_tag="VW144_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9600888.1"
                     /translation="MEPDIFIITKLLSDPSRMHILDILMDGKAHTVNEIASFTKIKQH
                     TVSYHLKLLTEAQVTTLQTYGRFHYYSLKSAAIAEMLEFLSFYSPQRDVKSYKQHIHK
                     KELKVARTCYDHIAGELGISITNYLLQENLLSESEKDFQLTEAGKSYFQRKLHIDTDE
                     LKKKKRKFCPKCLDWSERKNHVGGALGNALLEFFSEKQLVIPAQTPRSLTITEKGKEF
                     LEKEWGI"
BASE COUNT         5671 a         3113 c         3950 g         5497 t
ORIGIN      
        1 ggtaaaataa gaaaattgtt tatatattgt ctccgtagtg ttattatacg aaataaaaga
       61 ttttaggaaa agtcgttata tcatgctata cctattcttt gtggtataat tgcaagaggt
      121 ttaatccgat aatttataaa gagagaaaga tattcatgag agatttatta tctaaaaaaa
      181 gtcataggca attagaatta ttagaattat tatttgaaca taaacgttgg tttcatcgtt
      241 ctgaactagc agagttatta aattgtacag aacgtgcagt caaagatgat ctatcccatg
      301 ttaaatctgc ttttcctgac ttgatttttc attcttctac taatggtata cgcattatta
      361 ataccgatga tagtgatatt gaaatggttt accatcattt ctttaaacat tcaactcatt
      421 tttcgatttt agaattcatc ttctttaatg aaggttgtca agctgagagt atttgtaaag
      481 aattttatat cagttcatct tcgctctatc gtattattag ccaaatcaat aaagtgatta
      541 aaaggcaatt tcaatttgaa gtcagtttga cccctgttca aatcattgga aatgagagag
      601 acattcgtta cttttttgca caatattttt cagaaaaata ttatttccta gaatggccat
      661 ttgaaaattt ttcatcagag ccactatctc aattgttaga attggtttat aaggaaacaa
      721 gctttccaat gaatttgtca acgcatagaa tgctaaagtt gctcctagtt acgaacctat
      781 atagaataaa gtttggtcat tttatggaag tagataaaga ttcttttaac gaccaaagtt
      841 tggatttttt aatgcaggca gaaggaatag aaggtgttgc tcagagtttt gaatcagaat
      901 acaatatctc tttagatgaa gaagttgttt gccaattatt tgtgtcttac tttcaaaaaa
      961 tgtttttcat agatgaaagt ctctttatga aatgcgtaaa aaaggatagt tatgttgaaa
     1021 aatcttacca tctattgagt gattttattg atcagatttc agtcaagtat cagattgaaa
     1081 tggagaataa ggataatctg atttggcatc tgcataatac cgcacatttg tatcgtcaag
     1141 agttgtttac tgagtttatt ttatttgatc aaaaagggaa tacaatcagg aactttcaaa
     1201 atatttttcc aaaatttgtt tcagatataa aaaaagagct ttctcactat ttagagactc
     1261 ttgaggtttg ttctagttca atgatggtaa atcatctatc ttataccttc atcactcata
     1321 ccaaacattt ggtgattaat ttgctacaaa atcagcctaa actaaaggtt ttggttatga
     1381 gtaattttga tcagtatcat gcaaaattcg ttgcagagac actttcttat tactgtagca
     1441 ataattttga actggaagtt tggactgaat tagaattatc aaaggaatct ttagaagatt
     1501 caccttatga tatcattatt tctaatttta ttattcctcc tattgaaaat aagagactca
     1561 tctattcaaa taatataaac acggtctcac tcatatattt gttaaatgcc atgatgttta
     1621 ttcgattaga tgagtaacca aaaataacaa aaaacgctat ttcgcgtttg aactgtacct
     1681 caaaagttag acagaaaaaa tctaattttt ggggtgtttt tgttaagaaa agaccttcaa
     1741 tcatttaagc aaaatgtaca tctagattta tagccctcta aaactcttgc tttttttgta
     1801 gaagatacga aaaaagccat aaaaccaagg cttttcctgt tttatgtaga tgtttacaca
     1861 tgaaaattga agtattttca aaagctaatt ggagtgattt tattcatgat gggataaatg
     1921 atctggccaa aaatgattga gaaatagttg cttaaaaagc atttgcatga tactatatat
     1981 tgaaaggagg tgataatatg gtagaacaaa gaaaatcaat taccatgaaa gatgttgctt
     2041 tagaagcagg agttagtgtt ggaactgttt cacgtgtaat taataaagaa aaaggcatta
     2101 aagaagtaac tttgaaaaaa gtggaacaag cgattaaaac tttgaattac attccagatt
     2161 actacgctag aggaatgaaa aaaaatcgaa cagaaacgat tgcaatcatt gtaccaagta
     2221 tctggcatcc cttcttttca gaatttgcta tgcatgtgga aaatgaagtc tataagagaa
     2281 ataacaaatt actcttatgt tctatcaatg gtacaaatag agagcaagac tatctggaga
     2341 tgttgcgtca taataaagtt gatggagtgg ttgccattac ctatagtcca attgaacatt
     2401 acttgacgtc aggaattccc tttgttagta ttgaccgcac atactcagat attgccattc
     2461 cttgtgtttc atcagataat gatgcaggtg gaagagaagc agctaaacaa ttaattagta
     2521 aaggatgtca gcatttagcc tttgtgggcg gacataatac aaccattaat gaaaccaaac
     2581 gtcgtagaat ttcatttgaa aagtatgtcc aagagcataa aatacctctt agtatttttg
     2641 atttggatga gacagttact gactattatg gaaagctaga gagtttcttg gaagaaaatt
     2701 cttctataga tggaattttt acaatcaacg attttacagc tttggatgtg attgaaatac
     2761 tagaaaaaaa tggcaaacgt attcctcatg atgtccaaat tattggatac gatggaatta
     2821 aaatggctgg agatagagat tatctacttt caactattga acaaccattg gaagaaatgg
     2881 caaaagaagc ggttcgtatt ttgtttgata taattgatgg aaagactgtt aatttgcaga
     2941 caatcctacc agtaaaattt gttgaaggaa aaacaacaaa aaatgaaaat aagtcttgac
     3001 agaaagcgct atcaatgata gaatgaattc agataaaaag atttattttt aaaacaaaaa
     3061 tgaaacgttt caaaaaaaga aataaagaga cagcgccaag cgctatcttt tctagaaaaa
     3121 aaatgaaacg tttcaaaaag gaggttgcta tgaatagcaa agcgaagcaa gtttctcttt
     3181 gggaaagaat caagaaacaa aaactcttgt tattgatgac tgtccctggt ttagttttaa
     3241 cctttatctt taaatacatc cctatgtatg gggttttaat tgcatttaaa gattacaatc
     3301 ctttaaaagg aattttaggg agtgattgga ttggtttttc tgagtttaca aaattcatat
     3361 cctctcccaa ctttggtatc ttgttagcca acacattaaa attaagtatc tatggtttat
     3421 tgcttggctt tttaccacca atcattcttg ctattatgct caatcaactc ttgagtgaaa
     3481 aagtcaaaaa acgaattcag ctcattttat acgcaccaaa ctttatctca gtcgttgtta
     3541 ttgtcggtat gattttcctc ttcttttcag tgggaggacc aatcaacaat tttctttcta
     3601 tgtttggaat gaaggctgac ttcttgacaa atccagactt ctttagacct ttatacatct
     3661 ttagtggtat ctggcaagga atgggctggg cttcaacgct ctacacggca acattggtaa
     3721 atgtagatcc agccttagta gaagcagccc gactggatgg agccaatatc ttccaacgaa
     3781 tctggcacat tgatattcca gctcttaagc ctattatggt tatccaattt gttttagctg
     3841 caggtggaat tatgaatgtc ggatatgaaa aagcattctt gatgcagaca tcgttaaatt
     3901 tgccaacttc tgaaattatc tcgacatatg actataaagt tggtcttgta tcaggagact
     3961 attcttactc aacagcggtt ggtttgttta atgcagtgat taacgtagta ttgcttgttg
     4021 cagttaacca aatcgttaaa cgcatgaata atggtgaagg aatttaagga ggaaagtatg
     4081 aaaaattcga ttatggatac aaaatttgat agacgtatct tactcttaaa taaaatcatt
     4141 attgtcttta tcgttttgat gactttgctt cctttacttt atatcgtcgt agcatccttt
     4201 atggatccta aggttctggt tagtagaggg attagcttta atccagccga ttggactgta
     4261 gaaggttacc agcgtgtatt cagtgaccaa tctattctaa gaggttttat caattctcta
     4321 ctatactctt ttggatttgc agctttaaca gtcttgctat ctgtgtttac agcttatcct
     4381 ctttccaaaa aagatttggt agggcgccgt tggattaact acttcttgat tgtgactatg
     4441 ttctttggtg gtggtttagt cccaacttac ttgctcgtaa aagaattggg aatgctcaat
     4501 actccatggg ctatcattgt tccaggtgct gtcaacgttt ggaatattat tcttgctagg
     4561 gcctatttcc aaggattgcc tgaagaatta gttgaagctg ctgtcattga tggtgcaaat
     4621 gatttacaga ttttcttcaa aatcatgctt cctcttgcaa aaccaattat gtttgttctc
     4681 ttcctttatg cttttgtagg acagtggaac tcatactttg atgcaatgat ttatatcaag
     4741 gatccaaact tggaaccatt gcaacttgta cttcgtaaaa ttctcattca gagccaacca
     4801 ggtcaagaca tgattggagc acaagcggct atgaatgaaa tgaaacgttt agctgaattg
     4861 attaaatacg caactattgt catttccagc ttgccattga ttgttatgta tccattcttc
     4921 caaaaatact ttgataaagg aattatggct ggttcactta aaggataaaa aaagaaaaaa
     4981 taaaaggagt tttctcatga aattcaaaac attctcaaaa tcagcagttt tgttgacagc
     5041 tagtttagca gtacttgcag cctgtggctc aaaaaataca gcttcaagtc cagattataa
     5101 gttggaaggt gtaacattcc cgcttcaaga aaagaaaaca ttgaagttta tgacagccag
     5161 ttcaccgtta tctcctaaag acccaaatga aaagttaatt ttgcaacgtt tggagaagga
     5221 aactggcgtt catattgact ggaccaacta ccaatccgac tttgcagaaa aacgtaactt
     5281 ggatatttct agtggtgatt taccagatgc tatccacaac gacggagctt cagatgtgga
     5341 cttgatgaac tgggctaaaa aaggtgttat tattccagtt gaagatttga ttgataaata
     5401 catgccaaat cttaagaaaa ttttggatga gaaaccagag tacaaggcct tgatgacagc
     5461 acctgatggg cacatttact cattcccatg gattgaagag cttggagatg gtaaagagtc
     5521 tattcacagc gtcaacgata tggcttggat taacaaagat tggcttaaga aacttggtct
     5581 tgaaatgcca aaagctactg atgatttgat taaagtccta gaagctttca aaaacgggga
     5641 tccaaatgga aatggagagg ctgatgaaat tccattttca tttattagtg gtaacggaaa
     5701 cgaagatttt aaattcctat ttgctgcatt tggtataggg gataacgatg atcatttagt
     5761 agtaggaaat gatggcaaag ttgacttcac agcagataac gataactata aagaaggtgt
     5821 caaatttatc cgtcaattgc aagaaaaagg cttgattgat aaagaagctt tcgaacatga
     5881 ttggaatagt tacattgcta aaggtcatga tcagaaattt ggtgtttact ttacatggga
     5941 taagaataat gttactggaa gtaacgaaag ttatgatgtt ttaccagtac ttgctggacc
     6001 aagtggtcaa aaacacgtag ctcgtacaaa cggtatggga tttgcacgtg acaagatggt
     6061 tattaccagt gtaaacaaaa acctagaatt gacagctaaa tggattgatg cacaatacgc
     6121 tccactccaa tctgtgcaaa ataactgggg aacttacgga gatgacaaac aacaaaacat
     6181 ctttgaattg gatcaagcgt caaatagtct aaaacactta ccactaaacg gaactgcacc
     6241 agcagaactt cgtcaaaaga ctgaagtagg aggaccacta gctatcctag attcatacta
     6301 tggtaaagta acaaccatac ctgatgatgc caaatggcgt ttggatctta tcaaagaata
     6361 ttatgttcct tacatgagca atgacaataa ctatccaaga gtctttatga cacaggaaga
     6421 tttggacaaa attgcccata tcgaagcaga tatgaatgac tatatctacc gtaaacgtgc
     6481 tgaatggatt gtaaatggca atattgatac tgagtgggat gattacaaga aagaacttga
     6541 aaaatacgga ctttctaatt acctcgctat taaacaaaaa tactacgacc aataccaagc
     6601 aaacaaaaac tagaggttga ttatgggaga taagaaatac acagtagaaa aagccaatcg
     6661 ttttatagca gaaaataaac atctcgttaa tactcaatat aagcctgaag aacatttttc
     6721 agctgagatt ggttggatca atgatccaaa tggatttgtc tattttcgtg gagaatacca
     6781 tctcttttat caattctatc catatgatag tgtttgggga cctatgcact ggggacatgc
     6841 taaaagtaag gacttggtga cttgggagca cttgccagtg gcacttgctc ctgatcaaga
     6901 ttatgaccga aatggttgtt tctcaggctc tgccattgtc aaggatgatc gcctctggct
     6961 catgtacact gggcatatcg aagaaaaaac cggtgtccgc caagtgcaaa atatggcatt
     7021 ttcagatgac gggattcact ttgaaaagat ttcccaaaat ccagttgcaa ctggatcaga
     7081 cttgccagat gagttgattg ctgctgattt ccgtgatcca aaactctttg aaaaagatgg
     7141 acgctattac tccgtagtag ctgccaaaca caaggataat gtgggctgta tcgttctact
     7201 agggtccgat aacctagtag aatggcagtt cgaatccatc tttttaaaag ggggagaaca
     7261 ccaaggcttt atgtgggaat gcccagatta cttcgagtta gatgggaaag attgccttat
     7321 tatgtcaccc atgcgttatc agcgtgaggg agactcatat cataacatca actcatcgct
     7381 tttgttcacg ggtaaggtag attggagaga aaaacgtttt atcccagaat cagttcaaga
     7441 aattgatcat ggccaagact tctatgcgcc tcaaacattg ttggacgatc aaaatcgtcg
     7501 tatcctgatt gcttggatgc agacatgggg gcgtaccctt ccaacccatg accaagaaca
     7561 caaatgggca tgtgctatga ctttacctag aattctaaga ttggaagatg gcaaactaag
     7621 acaattccct gttaaaaaag gccaatatca aatccaaata gataaagatt gtcattacca
     7681 cttaggaaat gatacagatt atcttgaatt tggttatgac agtaatgcgc agcaagttta
     7741 cattgatcgt agccatctta ttcaaaaaat tctaggtgaa gaagaacagg acactagtcg
     7801 acggtatgta gatattgaag ctaaagaatt ggaagttgtt ctagataaaa attccatcga
     7861 gatttttgtc aatcaaggtg aagcaagctt gactgcaact tattacttaa cggtgccagc
     7921 tgagctatca cgaattgatt aaaaattaag ttatttctcc taaagaaaaa gttctctttc
     7981 taaaatagtg gaaagaggac tttttgtgtt ttgggtatat aagcttagtt tatggtattt
     8041 gtaaaattgg tgttggatta tgatttaagc tagttttcta aagaatttga aaaaaatttt
     8101 atttaagcaa aaaaaccttg gttccaaggc ttttcctgtt gtatgtagat gccccctaca
     8161 gggatcgaac ctgtgaccca cggattaaga gtccgctgct ctgccagctg agctaaggag
     8221 gcaaagaaaa aagctgtatt ggtgccgaaa cttcacggtt tgtattgaac ccgcgcaatt
     8281 aagcaggtgg gcaactcgct ctaactgaag ctgtttccgt gtgagacggc ctacatgctg
     8341 ttagaagact tttgtttccc taataataca aaaaatagtc ggtcaacact taagtgtgaa
     8401 gtcgtacacc acagcgtttc tatacttata tgataccact ttttcaaaaa aaatcaagag
     8461 gaaagtgcaa ttttttgaaa aggatttcag atttttcgca aacgatcgat agcttccttt
     8521 ctttgagcca aagcccgttc atatttacca gtatcttctg cttggaaata gtgatgatta
     8581 cgaatttttt ctggcagata gtcttgcttg atccaatttc caggatagtt gtgtggatag
     8641 agatagtctt gggcattccc cagttccttg cttccactgt agtgcccatc acgcaggtgt
     8701 cgcggaatag gcaagtgccc tgatgttttg aggtcagcaa gtgccttatc catagctaca
     8761 taggctgagt tggattttgg agaaagggcc aaatcaatca cgacattggc aatgagaatg
     8821 cgggcttctg ggaacccaat cttctgggca gcatccagag cagtcacggt atgaatctgg
     8881 gcctcaggat tggctaagcc gatatcttca taggcgataa cagtcaagcg acgagcgaga
     8941 ctaggcagat ccccagcctc aatcaagcgg gcagtatagt ggagactggc atccacatct
     9001 gagccacgaa tagacttttg cagggcagat agaacatcat agtgaccgtc tccatccttg
     9061 tccatagtga tgtagcttct ctgaagacta ttttccatga tgtctaaggt gatgtggcga
     9121 atgccctcgt cattctcagg ggtagagaga acagctaagt ccagtgagtt aaaggcagag
     9181 cgaaggtctc cgtttgtaga ggttgcgatg aaatccagcg catcctcatc tagttctatt
     9241 ggaaaatcaa aaccacgttc agggttactt agagctatct gcagggcctc tttgacgtct
     9301 tggttagaca gaggttccaa ctcgaaaatt tgaactcggc tacgaatggc aggagtgaca
     9361 gagaagaaag gattttcagt cgttgctcca atcatgatga ccagtccact ttccaagaga
     9421 ggcaagagga agtcttgctt ggtcttatct agtcgatgaa tttcgtctag taataggacg
     9481 agaccaccag aaaatttagc ttcttccgag atttcttgca gtcgcttttt actatcaact
     9541 gtcgcattga aagttcgaaa ggcatacttg gtcgttccag cgatggcaga ggcaatactg
     9601 gttttgccga ttccaggagg gccatataga atcatggagg acaggcggtt ggcttccacc
     9661 atgcggcgga tgatttttcc aggtccgacc agatgctcct gaccgatgac ctggtcgatg
     9721 gttttagggc gcatgcgaag cgcgagattg tctggcatag cagtcctttc taacgtggat
     9781 ttgagtagaa tgactcatct actatatctt actatgtcca ttctagccta ttagttgtga
     9841 tatttcaatg gttttgtaat aatcagggag tgagatagaa tcttttttac tatttaattt
     9901 aatgaggaga ggtatcagca tgagagattt gattttcctt aaagctcaaa ataggatagt
     9961 tgagcctaaa gaagtggaag tgctgacttg agaaataata ttgctgaatg tcattgtatg
    10021 aactataatg aaaagagagg tgataagcaa tggaaatgat ggaactacca agtcaggaaa
    10081 ttttgatttt tacaaaacaa attcgtcatt ggattcttag tgatcaagtt atttcaggga
    10141 aaagaaaact tttcttccgg gaagatactc caaaagagat tttagatttg tacgaaaaca
    10201 ttaaatctaa acttgatttt gcttaccaag aagttcattc taataatggg ttgaaaaaat
    10261 atgaaaaata gaataattga tgtttttgaa gtagtaaatc gcctccttgt aataactgtt
    10321 gaaaatcctg atttcgaaga cttgagagtt aatcatactc aatgaaaatc aaagagcaaa
    10381 ctaggaaact agccgcaggc tgtacttgag tacggtaagg cgacgttgac gtggtttgaa
    10441 tttgattttc gaagagtatc agtttgtaaa aattggagat aaaaaatata gagtacgcag
    10501 tgttcctatg attcattcaa ctccgcctca atctgtctta gatcgagata cttttacaat
    10561 tgactataca gatgatgaat tgctcgataa agaagcagtt tttactactc attagaaaaa
    10621 taggtatgaa taaattggct tcccttagaa ccacatctag gggcttttat gtataataga
    10681 ataataagta tcactccttt acaatacgtg taacacgtgt tataatggtg ttgtcaggag
    10741 gtaaaagcgc tatgcctatg acacaaaaag agatggttaa actccttacg gcccatggtt
    10801 ggataaagac tagaggcggt aaaggctccc atattaaaat ggaaaagcaa ggggaaagac
    10861 ctatcacaat ccttcatggt gagctgaata agtacactga aagagggata agaaagcaag
    10921 ctgggttgta aaattcagcc cctgcttttt gatatggagg tagtgaaatg ttagttacgt
    10981 atccagcttt attttattat gacgacacag acggaacaga agcgacttat tttgtccatt
    11041 tcccagattt tgaatactca gctacacaag gagaggggat ttctgaggct ttggctatgg
    11101 ggtcggagtg gctagggata actgttgcag atttgataga gagtgatgga gagttacctc
    11161 agccatcaga tatcaatagc ttgtctttaa ttgataatga tccttttaaa gatgatgaag
    11221 atttcgtgtc aacctatgac cttgataaat cttttatttc tatggtatct gttgatgtat
    11281 cagaatacct aggaagtcag gaacccatta aaaagacttt gaccatacca aaatgggcag
    11341 ataagttggg acgagaaatg gggcttaact tttctcagac tttgacagac gctattgcag
    11401 ataagaaagt tcaagcctaa gattaagcgt ggtatcgtgg taatagtcaa taaaaaagca
    11461 cgtccacttg tgctagttac tcgcctattg attttataga tttattagcc ctttcgaggg
    11521 ctttttatat tgatttttat gagaaatgaa gaaatcagac ttttaagaaa atcactgata
    11581 ccaagggttt aaatgaggta atatggtata attaggacat aaaataattt tgtggtaaga
    11641 tggtagtatc tattttagca tatttccgag caatcggggc gattaaagag tcgcatagaa
    11701 agaggacaaa atggcaacat acggattttt agatatttta gaggaagagt tggacaagaa
    11761 ctttcccttt gactttgaga ttagctggga caaacgcaac catgcggtag aagtgagttt
    11821 tctattggaa gcgcaaaatg ctgcaggtgt ggagatggtg gatgaagacg gagaggtttc
    11881 atcagatgac attctttttg aggaagcggt ccttttctac aatcctgcca agtcaacagt
    11941 caatgaggaa gactatttga ctgttattcc ttacctgcct aaaaaaggat tttcccgtga
    12001 atttttagct tattttgcgc ttttccttaa agatactgct gaggttgggc tagatgtcct
    12061 catggacttt ttggaagacc cagaagcaga ggaatttgtc atggaatgga accaagaagt
    12121 ctttgaagaa ggaaaaattg gcttggaaaa gggagaattt tatccttatc caaaatacta
    12181 ggagttggtt ggaggtgtta tgaagaaaat tgggacttat ttggtttatg tgctagctgt
    12241 tgtctttatt atgctagctt ttgcttgtgg aacaatcgca tttgcagagt tggggtattc
    12301 cgcagtttta gcctttactt ttggttatgc cttcgctctt ctaagcatgt atttaatctt
    12361 tattcttcat gagctgggtc atgctttttg tggctacttg acaggctatc ggctggtggc
    12421 ttttggatta ggacatttta ttttgaccaa aaagtcaggc aggtttcatc ttagtagaac
    12481 agccattctg aaaaatgttg gtgctcaata cattggttta aaagaggatg aaagcgatca
    12541 aagaatcatc ctgatgcttt caggaggctt gatggttcat ctcggcttga tattattggc
    12601 gatattgttt ggatttttga caagaagctg gtattttgca gggacttgga tttttcttaa
    12661 tttatctttc ttcctaaata acattttgcc agtcgacatc accgatggag caaaaatttg
    12721 ggaattacta caacaccctg aaaataccaa atacgcctac ctggtattga gacattctgc
    12781 ccagaccttg ctagctcctc aagaatatga tttgaaagac tttgttcaag cagttcctga
    12841 ggatgcacga gggagttttg cggatggtgt attaggtatg caaggtgaag tttccatttt
    12901 ggaagggaag gaagaagtag ctaaacagca gtttcaagca ttacttgaga ggacaactac
    12961 tccgatgatg cagacagttg cccaactatc cctgcttcat atcgccttgt tggaaggaga
    13021 ttttgagcaa gcggagcaat atgctagtat tcgacaggtc aaatcgtttc tgtctctcaa
    13081 actgtccaat cttcaagtga tacaagcctg gtatcaatta aaggtgaaga aagatgtaga
    13141 tcgaactcgt aaggctctca agattgctag acagaaaatg gatagtagtc gaatgttgag
    13201 ggatgagaaa agatattatg aaaactggtt agccgagctg gaaaaggaaa tgttagaagg
    13261 aactgaagat ggaactagaa atttctgatt tcacaggttg caagattgct ttgttttgtg
    13321 gggataagct tttgactatc ttacgcgatg ataaggcaag tattccctgg gccaatatgt
    13381 gggaactgcc aggtggtgga cgtgaaggtg atgaaagtcc ttttgagtgt gtagcgcgtg
    13441 aagtttatga agaactgggc attcatctga ctgaagattg tctgctttgg agtaaggttt
    13501 acccaagtat gctttttgca gacaaacagt ccgtttttct agttggtcag ttgacacaga
    13561 accagtttga cagtatcgta tttggagatg aaggacaggg ctatcagctg atgaatgttg
    13621 aggaatttct tagttccagt caagttgtgc ctcagttgca agagagatta aaagattatt
    13681 taaaagtaag tgattagaaa ggatggttcg gttaaatttc taaactgaac ccgccctaaa
    13741 cacggtgcca aaaagataaa cttctcttag acacaagcgt cttcagagaa tttcctattt
    13801 tggttttgtg ttttacgggc ttggtattaa taatggaaac atggcaagag ttaaaagtta
    13861 cagtgaagcg tgagggagag gagttggttt ccaatctctt gattgagctg ggagcgcaag
    13921 gagtcgcaat cgaagacagt atggactatg tgggaaatgt ggaccgcttt ggagagattt
    13981 tcccagaggt ggagcagcaa gaagaaatcg tagtgacagc ctactaccct gacacggtgg
    14041 atgtgacagt ggttgaggcg gatttgcagg ctcgtctagc agaattgaca gattttatgg
    14101 atttgggaga gttaaaaata ggtacgactg ccttggctga ggaagactgg gcagacaact
    14161 ggaagaaata ctatgaacct gctcgcatca ctcatgattt gaccatcgtt ccctcttgga
    14221 cagactatga ggcgactgcg ggagaaaaaa ttatcaagct ggatcctggc atggcttttg
    14281 gtacgggaac ccacccaact accaagatga gcctttttgc cttggaacag gttcttcgtg
    14341 gtggcgaaac ggtgctagat gtgggaactg gttcagggat tctctctatt gccagctcgc
    14401 ttctgggggc taaagaaatt ttcgcctatg acctggatga tgtggcagtt cgtgtggctc
    14461 aggaaaatat tgagctcaac cctggaatgg aaaatatcca tgtagcggca ggcgatttgc
    14521 ttaaaggcgt ggagattgaa gcagatgtca tcgtggcaaa catcttggcg gatatcctca
    14581 ttcatctgac agaggatgct tatcgcttgg ttaaggatga aggttacctg atcatgagtg
    14641 gcattatcaa ggataagtgg gacatggtgc gcgagttggc tgaatcagct ggatttttcc
    14701 tcgaaactca catggttcaa ggggaatgga atgcctgtgt ctttaagaaa accaaggata
    14761 tctctggtgt gattggaggc tagcatgcag cagtattttg taaaaggcag tgctatctct
    14821 cctgtcacta tcgagaacaa ggaaaccagt aagcatatgt ttcaggttat gcgcttgaaa
    14881 gaagaggatg aggttacctt agtctttgat gatggcatca agcgcttggc gcgcgtgctg
    14941 gatatggaaa atcgtcagtt tgagttggtc caagaattaa atgacaatgt ggaactacca
    15001 gtccaagtga ccatcgcatc tggatttccc aagggagaca agctggagtt catcgctcaa
    15061 aaagtaaccg aactgggtgc tagccaaatc tgggcctttc ctgcagactg gtcagttgcc
    15121 aagtgggatg gcaagaaatt gggtaaaaaa gttgaaaaac tagaaaaaat tgcccttgga
    15181 gcagccgagc aaagcaagcg taatattgta ccaagtatta aacttttcga gaaaaaagca
    15241 gattttctag ctcagctgga ccagtttgac tctatcatag tagcctatga agaatcagct
    15301 aaagaaggag aagccgctgc gcttctgcaa gcagtcactg gtcttgaaac aggagccaaa
    15361 ctgctcttta tctttggtcc agaaggcggt ctgtcacctg cagaaatcga aagttttgaa
    15421 gctaagggag ccgttttggt aggcctaggt cctcgtattt tgcgagcaga aacagcaccg
    15481 ctttacgcct tatcagccct tagtgtttta gtagaattag agaaataaga ggaagaaaat
    15541 ggaacaaaaa caccgttcag aatttccaga gaaggaactc tgggacttaa cagccctata
    15601 ccaagaccgt gaggatttct tgcgtgcaat cgagaaagct cgcgaagaca tcaaccagtt
    15661 tagccgtgat tacaagggca atcttcacac ttttgagaat ttcgagaagg cctttgcgga
    15721 attggaacag atctacattc agatgagcca tattggcaac tatgctttta tgcctcagac
    15781 gacggactat agcaatgacg aatttgccaa tattgcccaa gctgggatgg aatttgaaac
    15841 agatgccagc gtagccttga ccttctttga cgatgccttg gtggtagcag acgaggaagt
    15901 cttggaccgt ttgggtgaat tgcctcactt gacggcagct attcgtcagg ccaaaatcaa
    15961 aaaagcccac tatctagggg ctgatgtgga gaaggccttg accaatctcg gtgaagtttt
    16021 ctatagtccg caggacattt atactaagat gcgagctggg gattttgaaa tggctgactt
    16081 tgaagcccat ggcaagacct acaaaaacag ctttgtcacc tatgagaatt tctaccaaaa
    16141 tcacgaggat gctgaggttc gtgagaaatc cttccgttcc ttctcagagg gacttcgtaa
    16201 gcaccaaaat acggctgcag cagcctatct ggctcaggtc aagtctgaaa aactcttggc
    16261 tgatatgaag ggatatgact ctgtctttga ttatcttcta gctgaacaag aagtggaccg
    16321 tgtcatgttt gatcgccaga ttgacctcat catgaaggac tttgcaccag tcgctcagag
    16381 atacctcaag catgttgcca aggtaaatgg tcttgaaaag atgacctttg cagactggaa
    16441 attggacttg gacagcgccc tgaatcctga agtgactatt gacgatgcct atgatttggt
    16501 catgaagtcg gtagaacctt tggggcaaga atattgtcag gaagttgctc gttaccaaga
    16561 agagcgctgg gtggactttg ctgctaacag tggcaaggat tccggtggtt atgcggcgga
    16621 cccatatcgc gtacaccctt atgtcctcat gagctggaca ggccgtttga gcgatgtcta
    16681 taccttgatt catgaaatcg ggcattctgg tcaattcatc ttttcagaca atcatcaaag
    16741 ttacttcaat gcccatatgt cgacctacta tgttgaagca ccgtcaacct tcaatgaatt
    16801 gctactcagt gattacttag agaaccaatc taatgaccca cgtcaaaaac gcttcgctct
    16861 ggctcatcgc ttgacagaca cctacttcca taactttatc acccacctct tggaagcagc
    16921 cttccagcgt aaggtgtata cattgattga agaaggagaa accttcggag caagcaaact
    16981 caacagtatt atgaaggaag ttttgacaga tttttggggc gatgccattg aaatcgacga
    17041 cgatgcagct ttgacttgga tgcgccaagc tcactactat atgggcttgt atagttacac
    17101 ttactcagca ggactagtta tctcgactgc tggttacctt catctaaaac attctgaaac
    17161 tggagctgaa gactggctca atctccttaa atcaggtggt agcaagacac cacttgagtc
    17221 agccatgatt atcggtgctg atatttcaac agacaaacca ctccgtgata ccatccaatt
    17281 cttgtctgac acagttgacc agattatcgc ctacagtgct gagttggggg agtaagggga
    17341 tagagaggtt gttatagaat gggtatggac agttatttgc ttaaaattcc atgtttaaat
    17401 cccccattcc ttttcgagga attctttgcc cttttctgta atggtaagtg aacgtggcgt
    17461 ttgtgcaggg atgaccaatt gtttttcact gaaaaattct aaaagagcat tgccgagagc
    17521 tccgccgaca tgatttttac gttcgctcca atccaaacat ttagggcaaa atttccgttt
    17581 tttctttttc aattcatccg tatcaatgtg taattttctt tggaaataag acttcccagc
    17641 ttcagtgagt tggaagtctt tttctgattc gctgaggaga ttttcttgca agagataatt
    17701 ggtaatggaa atgccaagct caccagcaat atggtcatag caggtgcgag cgacttttaa
    17761 ttcctttttg tgaatgtgtt gtttataact tttgacatca cgctgaggag agtagaagct
    17821 gagaaattcc agcatttctg caatggcagc actttttaaa gaataataat gaaaacgtcc
    17881 ataggtttgt agagtggtta cctgcgcttc ggtaagtaat ttgaggtgat agctgacagt
    17941 atgttgcttg attttggtaa agctggcaat ttcgttaacc gtgtgagctt ttccatccat
    18001 tagaatatct aaaatgtgca tccgagaagg gtctgataag agttttgtga tgatgaaaat
    18061 atctggttcc ataaaaattt tctccaaatt tcatatatcg atgattttct atcttttatt
    18121 atactataat taaagcacaa gaaaaataga ttttaaaaat gttttactag agaatatttt
    18181 aaattttatt gtctgagttt aaatggaggt aaaattacaa aagtaattgc a
//
LOCUS       DAVKJJ010000039        17437 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000039 DAVKJJ010000000
VERSION     DAVKJJ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17437
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.039"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            5..75
                     /locus_tag="VW144_001449"
     tRNA            5..75
                     /locus_tag="VW144_001449"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Gly,seq:tcc)
     gene            110..183
                     /locus_tag="VW144_001450"
     tRNA            110..183
                     /locus_tag="VW144_001450"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:144..146,aa:Ile,seq:gat)
     gene            266..355
                     /locus_tag="VW144_001451"
     tRNA            266..355
                     /locus_tag="VW144_001451"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:302..304,aa:Ser,seq:tga)
     gene            369..442
                     /locus_tag="VW144_001452"
     tRNA            369..442
                     /locus_tag="VW144_001452"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:403..405,aa:Met,seq:cat)
     gene            446..518
                     /locus_tag="VW144_001453"
     tRNA            446..518
                     /locus_tag="VW144_001453"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:479..481,aa:Phe,seq:gaa)
     gene            531..611
                     /locus_tag="VW144_001454"
     tRNA            531..611
                     /locus_tag="VW144_001454"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:565..567,aa:Tyr,seq:gta)
     gene            616..686
                     /locus_tag="VW144_001455"
     tRNA            616..686
                     /locus_tag="VW144_001455"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:648..650,aa:Trp,seq:cca)
     gene            701..773
                     /locus_tag="VW144_001456"
     tRNA            701..773
                     /locus_tag="VW144_001456"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:734..736,aa:His,seq:gtg)
     gene            784..855
                     /locus_tag="VW144_001457"
     tRNA            784..855
                     /locus_tag="VW144_001457"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:816..818,aa:Gln,seq:ttg)
     gene            865..948
                     /locus_tag="VW144_001458"
     tRNA            865..948
                     /locus_tag="VW144_001458"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:899..901,aa:Leu,seq:caa)
     gene            1055..2332
                     /locus_tag="VW144_001459"
     CDS             1055..2332
                     /locus_tag="VW144_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEU9600889.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            2408..3892
                     /locus_tag="VW144_001460"
     CDS             2408..3892
                     /locus_tag="VW144_001460"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU9600890.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTTELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            3953..5233
                     /locus_tag="VW144_001461"
     CDS             3953..5233
                     /locus_tag="VW144_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9600891.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTIVSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMNLKG"
     gene            5237..5857
                     /locus_tag="VW144_001462"
     CDS             5237..5857
                     /locus_tag="VW144_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9600892.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            6023..7135
                     /locus_tag="VW144_001463"
     CDS             6023..7135
                     /locus_tag="VW144_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9600893.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEVTKTVIASEEVAPST
                     GTSVPEEQTTETTRPVEEATPQETTPAEKQETQASPQAASAVEVTTTSSEAKEVASSN
                     GATAAVSTYQPEETKVISTTYEAPAAPDYAGLAVAKSENAGLQPQTAAFKEEIANLFG
                     ITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGISYIIWKQRFY
                     APFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            7458..7883
                     /locus_tag="VW144_001464"
     CDS             7458..7883
                     /locus_tag="VW144_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU9600894.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAIQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIKVKEEMESILTEFERTELSRLLNKLVLGIENIEI"
     gene            7894..8295
                     /locus_tag="VW144_001465"
     CDS             7894..8295
                     /locus_tag="VW144_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEU9600895.1"
                     /translation="MSIITIILTTIVALEHFYIFYLESIATRSDATSRVFNMEKEELA
                     HPSVSSLFKNQGIYKALLGVFLWYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKIHLKVDSNLANPF"
     gene            8370..9014
                     /gene="pcp"
                     /locus_tag="VW144_001466"
     CDS             8370..9014
                     /gene="pcp"
                     /locus_tag="VW144_001466"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU9600896.1"
                     /translation="MKVLVTGFEPFGGEKGNPTLEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSVTPERVAINQDDARISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKFPYVK
                     AGFMHIPYMMEQVVNRPTTPTMSLVDIRRGIEAAIGAMIEHGDQELKLVGGETH"
     gene            complement(9042..9899)
                     /locus_tag="VW144_001467"
     CDS             complement(9042..9899)
                     /locus_tag="VW144_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEU9600897.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQAYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFILDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            10029..11171
                     /gene="tgt"
                     /locus_tag="VW144_001468"
     CDS             10029..11171
                     /gene="tgt"
                     /locus_tag="VW144_001468"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU9600898.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            11272..13089
                     /locus_tag="VW144_001469"
     CDS             11272..13089
                     /locus_tag="VW144_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEU9600899.1"
                     /translation="MRIKWFSLVRITGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNHEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     ASVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAVAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPIVQTLRPLPYIHAILAAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            13242..14393
                     /gene="nagA"
                     /locus_tag="VW144_001470"
     CDS             13242..14393
                     /gene="nagA"
                     /locus_tag="VW144_001470"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEU9600900.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14556..15614
                     /locus_tag="VW144_001471"
     CDS             14556..15614
                     /locus_tag="VW144_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU9600901.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15765..16130
                     /locus_tag="VW144_001472"
     CDS             15765..16130
                     /locus_tag="VW144_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEU9600902.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            16206..17147
                     /locus_tag="VW144_001473"
     CDS             16206..17147
                     /locus_tag="VW144_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEU9600903.1"
                     /translation="MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDIETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            17095..>17437
                     /locus_tag="VW144_001474"
     CDS             17095..>17437
                     /locus_tag="VW144_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU9600904.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKL"
BASE COUNT         5059 a         3115 c         4084 g         5179 t
ORIGIN      
        1 tttagcgggt gtagtttagt ggtaaaacta cagccttcca agctgttgtc gcgagttcga
       61 ttctcgtcac ccgctttgaa ctttgttctt tgtaccaagt ttttgacttg ggcgcgtagc
      121 tcaggtggtt agagcgcacg cctgataagc gtgaggtcgg tggttcgagt ccactcgtgc
      181 ccatagtgtt tagtccatta ctaggggatt ggaatattat ctgttcacta agaggacacg
      241 ggcttgttcc cgtataaact attttggagg attacccaag tccggctgaa gggaacggtc
      301 ttgaaaaccg tcaggcgtgt aaaagcgtgc gtgggttcga atcccacatc ctccttttat
      361 attattaacg cgggatggag cagctcggta gctcgtcggg ctcataaccc gaaggtcgta
      421 ggttcaaatc ctgctcccgc aataaggctc ggtagctcag ttggtagagc aatggattga
      481 agctccatgt gtcggcggtt cgattccgtc tcgcgccatt tatatatttt ggaagggtag
      541 cgaagaggct aaacgcggcg gactgtaaat ccgctccttc gggttcgggg gttcgaatcc
      601 ctccccttcc attttacggg catagtttaa aggtagaact aaggtctcca aaaccttcag
      661 tgtgggttca attcctactg cccgtgttaa tagaattatg gcgggtgtgg tgaagtggtt
      721 aacacaccag attgtggctc tggcatgcgt gggttcgatc cccatcactc gcctatttta
      781 tattggggta tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga
      841 atccagctac cccagttact atttgccggc gtggcggaat tggcagacgc gctggactca
      901 aaatccagtg tccgcaagga cgtgccggtt cgaccccggc cgccggtata gtatagtgtt
      961 aggaacgttg ttattcttcg ttcctttttt atattatttt tggtataatt atagttattc
     1021 aaattttatt tagattaaga aagtgtaggg gagtatgtct tgttctatcg atttattaaa
     1081 acatcggtat ttgaaaaata ttaaagaaaa tcctgaattg tttgtcggaa ttgagttgga
     1141 gtatcctgtt gcaagtttag aaggggatgc tacagatgtt gaagttataa aggatctatt
     1201 tcattattta gtttctactt tggatctcac cgtagcaaag gtagatgatt ttggcaatct
     1261 gatccagtta gtagatccga taagtcagga tgctatttta tttgaagttt cctatacaac
     1321 gattgagttt gcatttggta aggctgaaac gattcaagag gtcgaaaatc gtttcaataa
     1381 ttatatgaat gtaattcaga gaaagttagc tgaatcaaat catgctattg ttggctgtgg
     1441 tatccatccc aactgggata aaaatgagaa ttgtccagtg gcttatccac gctatcagat
     1501 gttgatggat tatttgaatt tgagtagaaa tattattaaa tcagatttac atcatttccc
     1561 tgaatatggt acttttatct gtgggagcca ggttcagctg gatatttcaa aaaccaacta
     1621 cttacgggtg attaatgctt ttactcaaat tgaagcggct aaggcttatt tatttgcaaa
     1681 ctctgaattt tcgggtgcgg attgggatac gaaaatttca agggatattt tctgggaaga
     1741 atctatgcat ggtatctatc cagagaatgt tggggtcaat gctagactcc ttaatgatga
     1801 agctgatttt tttgactatc taaatcattc tgcgattttt actgcggaac gtgatgggca
     1861 gacctattat ttttatccta ttcaggctgg ggactatttg gctacgtccg aaatccaagc
     1921 atttgctctg aatggggatg aggttattat ttacccccaa gagaaggatt ttgaaactca
     1981 tcgtagttac cagtaccaag atttaacgac tcgaggaaca gttgagtttc gtagtgtgtg
     2041 tacacagcca cttgatagga cttttgcttc tgcagctttt cacttgggat tattggttaa
     2101 tttagacaag ttagaagctt acttagaaac agcacctttc tttaaagtat ttggttatga
     2161 ttacaagtct ttaaggagac aattttctaa gaaaaatctt acagatgagg aagaaactac
     2221 gattattgaa ttttccaaag acttactcct actagctgag gagggactag tggtgagaaa
     2281 taaggaagaa atgacctatt tacagccttt gagagaagaa ttgagcctat aatttctctt
     2341 ataaagggag aattttctga aaaatcatga tataatggac gagactatag ataaaggata
     2401 gagagtaatg acattagttt atcaatcaac gcgtgatgcc aacaatacag taactgccag
     2461 ccaagcaatt ttgcaaggtt tggcgacgga cggcggtttg tttacaccgg ttacttatcc
     2521 aaaggtagat ttgaactttg acaaattgaa agatgcttct taccaggaag ttgctaagct
     2581 agttttgtca gcatttttag atgactttac agctgaggag ttggactact gtatcaacaa
     2641 tgcctacgat agcaaatttg atactccagc tattgcacca ttagtgaaat tagatgggca
     2701 atacaatttg gaacttttcc atggttcaac gattgccttt aaggatatgg ccttgtctat
     2761 tttgccatac tttatgacga ctgctgctaa gaaacatggt ttggagaaca agattgttat
     2821 cttgacagcg acatctggtg acacggggaa agctgctatg gcggggtttg cggatgtgcc
     2881 tggtactgag attatcgtct tttatccaaa ggatggtgtc agcaagattc aagagttaca
     2941 aatgaccact cagactggcg acaatactca tgttattgct attgatggta actttgacga
     3001 tgcgcaaaca aatgtgaagc acatgtttaa cgacgtggct cttcgtgaaa aattgactac
     3061 caacaagttg caattttcat cagctaactc tatgaacatt ggtcgtctgg tgccacaaat
     3121 tgtttattat gtttatgctt acgctcaatt ggttaagact ggtgaaattg tagctggtga
     3181 aaaggttaac ttcacagtac caacaggaaa ctttggaaat atcttggctg ccttttatgc
     3241 caaacaaatt ggtttgccag ttggtaaatt aatctgtgct tcaaatgaca acaatgtttt
     3301 gacagacttc tttaaaacac gtgtctatga caaaaaacgt gagtttaagg taacaaccag
     3361 cccatctatg gatatcttgg tatcttcaaa cttggagcgc ttgattttcc atcttttggg
     3421 aaataatgct gaaaagacaa ctgaacttat gaatgccttg aacacgcaag gacaatataa
     3481 gttgacagac tttgatgcag agattttgga cctctttgca gctgaatatg cgactgagga
     3541 agaaacggca gcagagatca agcgtgtttg tgagttagat tcttatatcg aggaccctca
     3601 tacagctgtt gcttcagcag tttataaaaa ataccaatcg gccactggag atgtaactaa
     3661 gacagtgatt gcttcaacag ctagtccata caagttccca gtagttgcag tagaagctgt
     3721 aactggaaaa gcaggtttaa cagactttga agccttggct caattacatg aaatctcagg
     3781 cgttgcagtg ccaccagcag ttgatgggct tgaaatagct ccaattcgtc acaagacaac
     3841 agtggcagct gctgacatgc aagcagcggt tgaggcttat ttaggacttt aagacagagg
     3901 gagcaaactc ggttgggaaa ccaactgagt ttcttttcat caggaggaga gattgtttaa
     3961 gaaaaataaa gacattctta atattgcatt gccagctatg ggtgaaaact ttttgcagat
     4021 gctaatggga atggtggaca gttatttggt tgctcattta ggattgatag ctatttcagg
     4081 ggtttcagta gctggtaata ttatcaccat ttatcaggcg attttcatcg ctctgggagc
     4141 tgctatttcc agtgttattt caaaaagcat agggcagaaa gaccagtcga agttggccta
     4201 tcatgtgact gaggcgttga agattacctt actattaagt ttccttttag gatttttgtc
     4261 catcttcgct gggaaagaga tgataggact tttggggacg gagagggatg tagctgagag
     4321 tggtggactg tatctatctc tggtaggcgg atcgattgtt ctcttaggtt taatgactag
     4381 tctaggagcc ttgattcgtg caacgcataa tccacgtctg cctctctatg ttagtttttt
     4441 atccaatgcc ttgaatattc ttttttcaag tctagctatt tttgttctgg atatggggat
     4501 agctggtgtt gcttggggga caattgtgtc tcgtttggtt ggtcttgtga ttttgtggtc
     4561 acaattaaaa ctgccttatg ggaagccaac ttttggttta gataaggaac tgttgacctt
     4621 ggctttacca gcagctggag agcgacttat gatgagggct ggagatgtag tgatcattgc
     4681 cttggtcgtt tcttttggga cggaggcagt tgctgggaat gcaatcggag aagtcttgac
     4741 ccagtttaac tatatgcctg cctttggcgt cgctacggca acggtcatgc tgttggcccg
     4801 agcagttgga gaggatgatt ggaaaagagt tgctagtttg agtaaacaaa ccttttggct
     4861 ttctctgttc ctcatgttgc ccctgtcctt tagtatatat gtcttgggtg taccattaac
     4921 tcatctctat acgactgatt ctctagcggt ggaggctagt gttctagtga cactgttttc
     4981 actacttggg acccctatga cgacaggaac agtcatctat acggcagtct ggcagggatt
     5041 aggaaatgca cgcctccctt tttatgcgac aagtataggc atgtggtgta tccgcattgg
     5101 gacaggatat ctgatgggga ttgtgcttgg ttggggcttg cctggtattt gggcagggtc
     5161 tctcttggat aatggttttc gctggttatt tctacgctat cgttaccagc gctatatgaa
     5221 cttgaaagga taggaaatgc aaaaaacagc ttttatttgg gatttagacg ggactttatt
     5281 ggactcttac gaagcgattt tatcagggat tgaggagact tttgctcagt tttctattcc
     5341 ttatgataag gagaaggtga gagagtttat cttcaagtat tcggtgcaag atttgcttgt
     5401 gcgggtggca gaagatagaa atctggatgt tgaggtgcta aatcaggtgc gtgcccagag
     5461 tctggctgag aagaatgctc aggtagtttt gatgccaggt gcgcgtgagg tgctagcttg
     5521 ggcagacgaa tcaggaattc agcagtttat atatactcat aaggggaaca acgcttttac
     5581 cattctcaag gacttggggg tggaatccta ttttacagag attttaacca gtcagagtgg
     5641 ctttgtgcgg aagccaagtc cagaagcggc tacctatctg ctagataagt atcagttgaa
     5701 ttctgataat acttattata taggggatcg gactctggat gtggaatttg cccagaatag
     5761 tgggattcaa agcatcaact ttttagagtc tacttatgaa gggaatcaca ggattcaagc
     5821 gttagcagat atttcccgta tttttgagac taagtgataa aaagattgtg tcagttttgt
     5881 gacagagacc taacaaacta tttcaagtaa cctagtttgt tacaaggaat agacagttct
     5941 gttaaatagg cccgagaggg ctttttttct acattttttg tgttatgata gacaggtact
     6001 catttgaaag gaatttgaaa gaatgaagaa aagaatgtta ttagcgtcaa cagtagcctt
     6061 gtcatttgcc ccagtattgg caactcaagc agaagaagtt ctttggactg cacgtagtgt
     6121 tgagcaaatc caaaacgatt tgactaaaac ggacaacaaa acaagttata ccgtacagta
     6181 tggtgatact ttgagcacca ttgcagaagc cttgggtgta gatgtcacag tgcttgcgaa
     6241 tctgaacaaa atcactaata tggacttgat tttcccagaa actgttttga caacgactgt
     6301 caatgaagca gaagaagtaa cagaagttga aatccaaaca cctcaagcag actctagtga
     6361 agaagtgaca actgcgacag cagatttgac cactaatcaa gtgaccgttg atgatcaaac
     6421 tgttcaggtt gcagaccttt ctcaaccaat tgcagaagtt acaaagacag tgattgcttc
     6481 tgaagaagtg gcaccatcta cgggcacttc tgtcccagag gagcaaacga ccgaaacaac
     6541 tcgcccagtt gaagaagcaa ctcctcagga aacgactcca gctgagaagc aggaaacaca
     6601 agcaagccct caagctgcat cagcagtgga agtaactaca acaagttcag aagcaaaaga
     6661 agtagcatca tcaaatggag ctacagcagc agtttctact tatcaaccag aagagacgaa
     6721 agtaatttca acaacttacg aggctccagc tgcgcccgat tatgctggac ttgcagtagc
     6781 aaaatctgaa aatgcaggtc ttcaaccaca aacagctgcc tttaaagaag aaattgctaa
     6841 cttgtttggc attacatcct ttagtggtta tcgtccagga gacagtggag atcacggaaa
     6901 aggtttggct atcgacttta tggtaccaga acgttcagaa ttaggggata agattgcgga
     6961 atatgctatt caaaatatgg ccagccgtgg cattagttac atcatctgga aacaacgttt
     7021 ctatgctcca ttcgatagca aatatgggcc agctaacact tggaacccaa tgccagaccg
     7081 tggtagtgtg acagaaaatc actatgatca cgttcacgtt tcaatgaatg gataaacccg
     7141 acttgataac atcattttga cgaatgagat ctagctttcg tgatggaaag cgattctcgt
     7201 tcgttttttc tttgtcatac tcttcgaaaa tctcttcaaa ccacgtcagt tttatctgaa
     7261 acctcaaagc tgtgctttga gcaacctgcg actagcttcc tagtttgctt tttgattttc
     7321 attgagtatc aatttgaatg gaaaatggaa agttatcatc ttgtaatgag ttaagcaaca
     7381 ttcttgcaat ctattttact ttatatcaca attaattagt caactattga taaatcaata
     7441 aaaagagagg ggaagaaatg ctagagattc aagatttact gtatcaactc cgcttgtctg
     7501 agcaagcgag tacgcaattg tttgaaaaaa ggcttgggat tagtttgaca cggtatcaga
     7561 ttttactgtt tttgctggag cattctcctt gtaaccaaat ggcgattcag gagcgtttga
     7621 aaattgatca ggctgctttg acacggcatt tcaagatttt ggaaacggaa ggtttggtgg
     7681 agcgtcatcg taatcctgaa aatcagcggg aagtgttggt agaggctgcg aagtatgcca
     7741 aggagcagtt agtggtgaat ccccctctgc aacatatcaa ggttaaggaa gagatggaaa
     7801 gtatcttaac agagtttgag agaacagaac tcagccgttt attaaataaa ttggttttgg
     7861 gtattgaaaa tatagaaatt taaggagaaa tagatgtcaa ttattacaat tattttaaca
     7921 acgatcgttg ctttggagca tttttacatt ttttatttgg aaagtattgc tacgcgatca
     7981 gatgcgacta gtcgtgtatt taatatggaa aaggaagaat tggctcatcc gtcagtaagt
     8041 tcattgttca aaaatcaagg gatttataag gctctgctag gagtctttct ctggtatggc
     8101 atttatttct cacagaattt agaaattgtg actatttttg tcttatttgt gattggtgct
     8161 gcgacttacg gctctttaac agcggataaa aaaattattt tgaagcaagg tggatcagct
     8221 attttggcct tgattagtat tttactcttt aaaatacact tgaaggtcga ttctaatctc
     8281 gctaatcctt tttaatccag aataagggaa atatgttata cttgttttta agaaaaaagt
     8341 ctcattgaat tggttttgag gagttagaaa tgaaagtatt agtgacaggt tttgagccct
     8401 ttggagggga aaagggcaat ccaactttgg aggccattaa aggtttacca gctgaaatcc
     8461 atggtgctga ggtccgttgg ctagaggtgc cgacagtttt tcacaaatct gctcaagtat
     8521 tggaagaaga gatgaatcgt tatcaacctg actttgtcct ttgtattggg caagctggtg
     8581 gaagaactag tgtgacacct gaacgagtgg ccattaatca agacgatgca cgcatttctg
     8641 ataacgaaga taatcaaccg attgaccgtc ccattcgccc agatggtgct tcggcctact
     8701 ttagtagttt gccgattaaa gcgatggttc aagctataaa aaaagagggc ttaccggcct
     8761 ctgtttccaa tacggcaggg acttttgtct gcagccattt gatgtatcag gctctctatt
     8821 tggtagaaaa gaaatttcca tatgttaagg caggttttat gcatattcct tatatgatgg
     8881 aacaggtggt gaacagaccg actactccaa ctatgagttt agtggatatt cggcgaggga
     8941 tagaagcagc aatcggcgct atgatagaac atggggatca ggaactcaag ttggtaggcg
     9001 gagaaactca ttgatagaaa aaagcttgag gggaaaaacc ttcaagcttt tggacgtttt
     9061 cgagccaata ctgctcggta aaacataatt ttagtgcatt ggatataagg taggagtgaa
     9121 aaactagcaa tgccaaaggt aatccaattg aggaagtacc aaggaagaag ctgtaaatct
     9181 aggataaagt gctggaactt gtagcccttc ataaaggaac ggctagtttt taggattcgt
     9241 cttggtggga cctgtcctag gtctagacta taacagagaa gaaattccac ctgtgaatag
     9301 gcataatact gtggaatata gaggatattt cctacaatga tcaagatgag acttgcaaga
     9361 aagtagagtc caaagaccat gaggaaacgc tcggtttcaa ctgatgagag atctagattt
     9421 ggaaactcag gatgtagggt gacgaatttt ttggctaaaa agctactata aaagaggaga
     9481 taaatcccaa gtaaattagg gatactccat aaaaagagat agaaacgttt gagaagtagg
     9541 gtcaaaaagg tttgagaaaa gcgctcctca tcaaagagag ctaggctgtt ttttacagat
     9601 ggctccgttt tagaatcttt catgagtgtc agtgttgcat agacggaact ggtcaaaaga
     9661 atagtcccga taaaggagac tagtagagga aagaggtagg cttgaagtat ttggccaagt
     9721 atgctgaaaa atggctgttc taaaacagtc ccgtggatcc gagataaggg attaagaaaa
     9781 ctagataaga tgaccagcat actgggaagg atatagagga gaaagagacg gggggtgtca
     9841 gcctgaaaat gttttgactc ctgacgaatt gtttttaaat caatttttgg atagttcatt
     9901 ctcttattat accatagttc ttatacatag ttcgtgacag ttcctacttt ttttgataaa
     9961 atcatacagt gtgtccttgg gcacactgta tgaactggga ctgtctttcc cagcttcgga
    10021 ggtaaaaaat gtcagattca ccaatcaaat atcgtttgat taagaaagaa aaacacacag
    10081 gagctcgtct aggagaaatc atcactcccc acggtacctt tccgacacct atgtttatgc
    10141 cagttgggac acaagccact gtcaaaactc agtcacctga agaattgaag gagatgggtt
    10201 cgggaattat cctatcaaac acttatcatc tctggcttcg ccctggagat gaactcattg
    10261 cacgcgctgg tggtctccac aagttcatga attgggacca gcctatcttg acagatagtg
    10321 gtggttttca ggtttattct ttagcagata gccgtaatat cacagaagaa ggagtaacct
    10381 ttaaaaatca tctaaatggt tctaagatgt tcctatcccc agaaaaagcc atctctattc
    10441 agaataatct gggttcagac atcatgatgt cctttgatga atgtcctcag ttttatcaac
    10501 cttatgacta cgttaagaaa tcgatagagc gtaccagccg ttgggctgag cgtggtttga
    10561 aggctcaccg tcgtccacat gaccaaggtt tgtttggaat tgtgcaaggt gcaggatttg
    10621 aagaccttcg ccgccaatca gctcatgatc ttgtcagcat ggatttctca ggctactcta
    10681 tcggtggttt ggcagtggga gaaacccatg aagagatgaa tgcggtcttg gactttacaa
    10741 ctcaactgct gcctgaaaat aaacctcgtt atctgatggg tgtgggagcg ccagatagct
    10801 tgatcgatgg ggtcattcgt ggggtggata tgtttgactg tgtcttaccg actcgaattg
    10861 ctcgtaacgg gacttgtatg accagtcaag gacgtttggt tgtgaaaaat gcccagtttg
    10921 ctgaggactt tacgccactg gatcctgagt gtgattgcta cacatgtaat aactatacac
    10981 gcgcttacct tcgtcacctg ctcaaggctg atgaaacctt tgggatccgc ttgactagct
    11041 accacaatct ttacttcttg cttaacctga tgaagcaagt gcgacaagcc atcatggatg
    11101 acaatctctt ggaattccgt gagtattttg tggaaaaata tggctataat aagtcaggac
    11161 gtaatttcta aaatggaatt gatataagct aaaaatccta agttttctct taggattttt
    11221 cttctttttt tgatagaata aagtgtacaa tgaaaggaag aataaactcg tatgcgcatt
    11281 aaatggtttt ccttggttag gattacaggt ttacttttgg tactcttgta tcacttcttt
    11341 cagacgatct ttcctggagg atttttcggg gtagatgtct ttttcacatt ttcaggtttc
    11401 ctgattacag ctctactcat tgaagaattt tctaaaaacc atgagattga tttgatagga
    11461 ttttttagaa gacgctttta tcggattgtg ccacctgtgg ttttgatggt cttggtgacc
    11521 atgcctttta ctttcttggt tcgccaagac tatgttgctg gaattggtgg ccagattgcg
    11581 agcgtcttag gctttatgac caacttctat gaactcctaa caggtgggag ttatgaatct
    11641 cagttcattc ctcatttgtt tgttcataat tggagtttgg cagttgaggt tcactactat
    11701 attctttggg gattggcagt ttggttctta tccaaacaag ctaaatcaaa tggtcagttg
    11761 aaggggatgg tctttctctt atctgctgtt gccttcttga tcagtttctt ctccatgttt
    11821 attggtagtt ttctagtgac ctcttattcc tctgtttatt tctccagttt aactcatgtc
    11881 tatccattct ttttgggaag tatgttagca actattgtag gcgttcgtca gacgacttcc
    11941 ctcgtcaagc agttggataa aatctgggat ttacgaaaga ctttggtagt ttttggagga
    12001 ggctttggtt tcctagttct tttgactttc tttgtcaaat tcacttatct ttttgcctat
    12061 cttatcggct tcttacttgc cagtcttgca gctcttgcca tgattctggc ggcgcgtgtc
    12121 ttacatgaaa agacacatca tatacaggag ccgaagatta tcagcttttt agcggatact
    12181 agctatgcgg tttatctttt ccattggcct ttctatatca ttttctcaca gttgacatca
    12241 aatcttcttg ctgtattact gactctgatt tgttcttatg gctttgccag tctgtcattt
    12301 tatgtattgg aaccttggat tgcaggcaag aacacaccta ttgtccaaac ccttcgtccc
    12361 ctgccttata ttcacgcaat tcttgcagca ggtacaggaa tcttgaccat cattgtctgc
    12421 acggtgacct tgttggcacc acaagtggga gcgtttgaga cagacttgac tgtcaatggc
    12481 ttgaagcaag ctgcaacaaa tattggccag accaaggtga tggcagaacg ggcagatgca
    12541 aacagtttgg gaattgctga tggcactatg ttaattggtg actcagtggc tttaagggca
    12601 aatacagcac tacagacagc tcttcctgga gcacagatta acgcgcaggt cagcgtaaca
    12661 accaagaccg caaatgaaat catgctaaat aatagccaga ataaattttt acctaagacg
    12721 gtggtcattg cgactggggt aaataatcct gagaattaca aggatgactg ggacagtatc
    12781 gtgaaaaatc ttcctaaggg acaccatatg attttggtga ctccttatga gggagataag
    12841 acaaaagaga cctatgccat cgttgagaag gctgctgcct atatgagaga attggcagag
    12901 aagacacctt acattacgat agcagattgg aatcaagttg cgaaagagca tccagaaatt
    12961 tgggcaggaa cagaccaggt tcatttcggg agtgagagta gcactatcga agcaggagca
    13021 aaattgtatg cagatacgat tgccacagct ttgcagacag ctcaagacaa gccggttaaa
    13081 tcaaaataac ttgtattaaa aaagagaaga gttggagaaa tccagctctt ttaaaatatc
    13141 tccaaaaaat aggtctatac catttacaaa taaaaaagaa aggtttataa tgtaattgac
    13201 ataataaatt gtagaatcaa tcttttaagg aggttaacat tatgcctaac tatattaaag
    13261 cggatcagtt tttctaccca cacggagttc gtcgaggtgg ttacttggaa cttgtggacg
    13321 gcaagtttgg gaaacatgta gagcagattc ctgaaggggc tgaggtgatt gactatacag
    13381 gttatagcat tgccccaggt cttgtggata ctcatattca tggatatgca ggtgtagatg
    13441 tgatggacaa caacattgaa ggtacattgc atactatgag tgaaggactt cttagtaccg
    13501 gtgttaccag tttcttaccc acaactttaa cagccactta tgagcaattg cttgcagtca
    13561 ctgaaaatct tggaaaccat tataaagaag caacaggtgc taagattcgt gggatttatt
    13621 atgaaggtcc atatttcaca gaaactttta agggggcaca aaatccaact tatatgagag
    13681 acccgggtgt tgaggagttt cattcttggc aaaaagcggc aaatggcttg cttaataaaa
    13741 ttgcccttgc accagaacgt gatggggtgg aagactttgt tcgtacagtt acgggcgaag
    13801 gtgtgacggt tgctcttgga cattcaaacg cgacttttga tgaagccaaa aaagcagtcg
    13861 atgctggagc gagtgtttgg gtgcatgcct acaatggaat gcgtgggttg actcaccgtg
    13921 aattgggtat ggttggagcc atgtaccaat tgccacatac ctatgcagag ttgatctgtg
    13981 atggtcacca cgtagatcca aaggcttgcg aaattcttat caaacaaaaa ggaacagaaa
    14041 acatcgctct tatcacagac tgtatgacag ctgggggatt ggaagacgga gattatatgt
    14101 tgggagaatt cccagttgtc gttgcaaatg gaactgcacg cctcaaatcg acaggtaact
    14161 tggcaggttc tatcctcaaa ctcaaagatg gtttgaagaa tgtggtcgaa tggggaattg
    14221 cgaatccgca tgaagcagtc atgatggcca gcttcaaccc agctaaatcc gttcacatcg
    14281 atgacgtctg tggccaaatc cgtgaaggct acgacgctga cttcatcgta ttagataaag
    14341 atttggaatt ggtagcaacc tacctagatg gcgtaaaacg ttatcaagca taagagagaa
    14401 agcagaagtt agagactaac ttctgctttt ttatgcaggg tactttactg gtaaataaaa
    14461 agttaaaaaa atcacaaaaa aagcttgaag aaacaaagaa aaaggcttac aattgaacta
    14521 taaatattac aaataaaaaa acggaggagt gctttatgaa agcctatact tatgttaaac
    14581 caggacttgc ttcttttgtt gatgtagaca aaccagttat tcgcaagcca acagacgcta
    14641 ttgtgcgtat tgtaaaaacc actatttgtg gaacagacct ccatattatc aaaggggatg
    14701 ttcctacttg ccaaagtggt accattcttg gccacgaagg gattgggatt gttgaagaag
    14761 ttggggaagg agtttccaac ttcaaaaaag gtgacaaggt cttgatttct tgcgtctgtg
    14821 cctgtggtaa atgctactac tgtaaaaaag gaatttatgc tcactgtgaa gacgaagggg
    14881 gctggatttt cggtcacttg attgatggta tgcaggctga atatctacgt gtccctcatg
    14941 cagataatac tctttaccat actccagaag acttgtcaga tgaagctttg gttatgctgt
    15001 cagacattct gcctactgga tatgaaattg gtgtcttaaa agggaaagta gaacctggtt
    15061 gcagcgtagc cattattggt tcaggtccag ttggattggc tgctctttta acagcccaat
    15121 tctattcacc agctaaattg attatggtag acctagacga taaccgcttg gaaactgccc
    15181 tatcattcgg tgcgactcat aaggttaatt cttcagaccc tgaaaaagcc attaaagaaa
    15241 tttatgattt gacagatggt cgtggtgtgg atgtcgctat cgaagctgtt ggtattcctg
    15301 caacatttga tttctgtcaa aagattatcg gtgtagacgg aacggttgcc aactgtggtg
    15361 tgcatggtaa accagttgaa ttcgatttag ataaactttg gattcgcaac atcaatgtaa
    15421 caactggttt ggtatctaca aatacgactc cacaattgtt gaaagcgctt gaaagtcata
    15481 agattgaacc agaaaaattg gtaactcact atttcaaact cagtgaaatt gaaaaagcct
    15541 acgaagtctt cagtaaggca gcagaccacc atgccattaa ggtcattatc gaaaacgata
    15601 tctcagaagc ctaagtagta aaaatatttt tgtacataag taaatagaaa ttcagtcatc
    15661 catcagatgg ctggattttt tatcaaaaaa ttaagaaatg agcatatttc tttccttgtc
    15721 tggcggaatt ggttataata tacggtacaa aggaatgaat gaatatgtat cgtgttatag
    15781 aaatgtacgg agattttgaa ccgtggtggt tcttagaagg ttgggaagaa gatattgtag
    15841 caagtagaaa atttgaccag tattatgatg ctctcaaata ctacaaaact tgctggttta
    15901 gattggaaca agaatcccct ctttataaaa gtagaagcga cttgatgacc attttttggg
    15961 acccggaaga ccaacgctgg tgtgatgaat gtgatgagta tttacaacaa taccattctt
    16021 tggctctttt gcaggatgag caggttatcc cagacgaaaa actacgctca ggctatgaaa
    16081 aacaaaccag tcaggaaagg aatcgttctt gccgtatgaa attaaaatag agaaaagtaa
    16141 cttttttgga gttgcttttt ttatttttct aacgctttgc gaatagtata ggtgaggagg
    16201 taagtatggt tcaagaaatt gcacaagaaa tcattcgctc ggctcggaaa aaaggggcgc
    16261 aagacattta ttttgtcccc aagttagacg cctatgagct tcatatgagg gtaggagacg
    16321 agcgctgtaa aattggtagc tatgattttg aaaagtttgc agccgttatc agtcacttta
    16381 agtttgtggc gggtatgaat gtgggagaaa aaagacgtag tcaactgggt tcctgtgatt
    16441 atgcctatga ccagaagatg gcgtctctac gtttatctac tgtaggcgat tatcgggggc
    16501 atgagagttt ggttatccgt ttgttgcacg atgaggagca ggacttgcat ttttggtttc
    16561 aggatattga agaattaggc aagcagtaca ggcaacgggg actctatctt tttgctggtc
    16621 cggttgggag tggtaagacg accttgatgc atgaattgtc caagtcactc tttaaaggac
    16681 agcaagttat gtccatcgaa gatcctgtcg aaatcaagca ggacgacatg cttcagttgc
    16741 agttgaacga agcaatcggt ctaacctatg aaaatctaat caaactttcc ttgcgtcatc
    16801 gaccagatct cttgattatc ggagaaattc gtgacatcga gacggcgcgt gcagtggtca
    16861 gagctagttt gacaggtgcg acagtctttt caaccattca cgccaagagt atccgaggtg
    16921 tttatgagcg tctgctggag ttgggtgtga gtgaagaaga attggcagtt gttctgcaag
    16981 gagtctgcta ccagagatta atcgggggag gaggaatcgt tgactttgca aacagagatt
    17041 atcaagaaca ccaagcagcc aagtggaatg agcaaattga ccagcttctt aaagatggac
    17101 atatcacaag tcttcaggct gagacggaaa aaattagcta cagctaagca aaaaaatatc
    17161 atcaccctat ttaacaatct cttttctagc ggttttcatc tggtggagac tatctccttt
    17221 ttagatagga gttccttgtt ggacaagcag tgtgtgaccc agatgcgtac aggcttgtct
    17281 caggggaaat cattctcaga aatgatggaa agtttgggat gttcaagtac cattgtcact
    17341 cagttatctc tagctgaagt tcatggcaat cttcacctga gtttgggaaa gatagaagaa
    17401 tatctagaca atctggccaa ggtcaagaaa aagttaa
//
LOCUS       DAVKJJ010000040        17066 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000040 DAVKJJ010000000
VERSION     DAVKJJ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17066
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.040"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(35..790)
                     /locus_tag="VW144_001475"
                     /pseudo
     CDS             complement(35..790)
                     /locus_tag="VW144_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567550.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
     gene            complement(928..2550)
                     /gene="groL"
                     /locus_tag="VW144_001476"
     CDS             complement(928..2550)
                     /gene="groL"
                     /locus_tag="VW144_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU9600905.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(2566..2850)
                     /gene="groES"
                     /locus_tag="VW144_001477"
     CDS             complement(2566..2850)
                     /gene="groES"
                     /locus_tag="VW144_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU9600906.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKAAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(3120..3515)
                     /locus_tag="VW144_001478"
     CDS             complement(3120..3515)
                     /locus_tag="VW144_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU9600907.1"
                     /translation="MYNKVILIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(3593..4354)
                     /locus_tag="VW144_001479"
     CDS             complement(3593..4354)
                     /locus_tag="VW144_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU9600908.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHVINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(4387..5013)
                     /locus_tag="VW144_001480"
     CDS             complement(4387..5013)
                     /locus_tag="VW144_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEU9600909.1"
                     /translation="MIFTYNKEYVGDVLMVIVKNSGDAKLDVERKGKVARVFLKDNGE
                     TVAWNIFEVSSLFEIAERGQVFLTDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNLIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(5029..5346)
                     /locus_tag="VW144_001481"
     CDS             complement(5029..5346)
                     /locus_tag="VW144_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU9600910.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKQQINDFLA
                     GLK"
     gene            complement(5343..5642)
                     /locus_tag="VW144_001482"
     CDS             complement(5343..5642)
                     /locus_tag="VW144_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEU9600911.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDLFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFIYENEDLVYEEEVL"
     gene            complement(5927..6118)
                     /locus_tag="VW144_001483"
     CDS             complement(5927..6118)
                     /locus_tag="VW144_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEU9600912.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(6279..6953)
                     /locus_tag="VW144_001484"
     CDS             complement(6279..6953)
                     /locus_tag="VW144_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600913.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDFVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLTIRGDVAFGKL
                     EIVYVK"
     gene            complement(6959..7405)
                     /locus_tag="VW144_001485"
     CDS             complement(6959..7405)
                     /locus_tag="VW144_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9600914.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(7507..8022)
                     /locus_tag="VW144_001486"
     CDS             complement(7507..8022)
                     /locus_tag="VW144_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9600915.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVCVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLFLHVS"
     gene            complement(8019..8381)
                     /locus_tag="VW144_001487"
     CDS             complement(8019..8381)
                     /locus_tag="VW144_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HEU9600916.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICSVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(8447..10306)
                     /locus_tag="VW144_001488"
                     /pseudo
     CDS             complement(8447..10306)
                     /locus_tag="VW144_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(10323..10793)
                     /locus_tag="VW144_001489"
     CDS             complement(10323..10793)
                     /locus_tag="VW144_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU9600917.1"
                     /translation="MDKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVHFLDN
                     REKNQDLVLIKDIEQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQANIKGIGGENKEISQKN"
     gene            complement(10917..11633)
                     /locus_tag="VW144_001490"
     CDS             complement(10917..11633)
                     /locus_tag="VW144_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9600918.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(12514..13929)
                     /gene="ply"
                     /locus_tag="VW144_001491"
     CDS             complement(12514..13929)
                     /gene="ply"
                     /locus_tag="VW144_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEU9600919.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(13940..14272)
                     /locus_tag="VW144_001492"
     CDS             complement(13940..14272)
                     /locus_tag="VW144_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600920.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDHLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(14343..14951)
                     /locus_tag="VW144_001493"
     CDS             complement(14343..14951)
                     /locus_tag="VW144_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600921.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(14964..15422)
                     /locus_tag="VW144_001494"
     CDS             complement(14964..15422)
                     /locus_tag="VW144_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEU9600922.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            15547..15946
                     /locus_tag="VW144_001495"
                     /pseudo
     CDS             15547..15946
                     /locus_tag="VW144_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(16710..16970)
                     /locus_tag="VW144_001496"
     CDS             complement(16710..16970)
                     /locus_tag="VW144_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600923.1"
                     /translation="MRERSATGAQDLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
BASE COUNT         5007 a         3872 c         3102 g         5085 t
ORIGIN      
        1 aatgttttag aagtaaaggt gtactattct agtttcaatc tactataaat ttgatatgag
       61 aatagtcttt ctcaacatcc ttacccaaca agaatgctgt agcgtccttc ttaacttgaa
      121 ccaggtcaat attctgggct tgggctgcat agacgcagcc acaataacat tgacgataga
      181 tatcatactc ctcacacatc tctactgaac gtttgtagcc ttgatttttc ttgaaatcgt
      241 tgggaagata gtgggtcgtg taaatttttt gcacatcgat tccgatgctg ttgatggttt
      301 gagaattctt atgaggactg atggtcaagg ctgaaccaaa gtagtcaaag cccaagtcca
      361 tagccacttg cgctgtttta tccagtcggt agtcaaaaca aaccttgcaa cggtcgccac
      421 cttcgggctc ctcctctagt cccctaacta gttttcggta ttaattgggt tcgtagggag
      481 cttctaggta ctgaaccgta tttcctgtcc gctcattaaa atcactaaca aatttcttgg
      541 tgacatagac ccgcttatgg tattctacct tgggatggat attagaattg gcaaaataga
      601 tggtcacatc tgcatacttg gtcaaatatt ctagtgtata ggtactacaa ggggcacagc
      661 aaacatgcac gagaatggtt ggccgttgct catttttcta ccatacttct accattttct
      721 gcatgacacg gtcataatta atcttctgat tggggggcat cttgcacaga atttcttcta
      781 catcgatcat gttcttctcc tttttctagt cttattttat catataagtt aggagagctc
      841 aaatcaattt agaagtgaat attataaaaa gaagggttag tcattccctc cttttgtgtt
      901 ttttataagt tgttttctat agaaagctta catcatcccg cccatcatac ttggatccat
      961 tgctggagct ggggctactg gttctggttt attggctacg actgcttctg ttgtcaaaat
     1021 caagcttgct acagatgctg cattttgtag ggctgaacga ctcactttaa ctggatcaat
     1081 gataccttga tcaatcatgt taacccactc gccagttgct gcgttgaatc ctataccaag
     1141 ctcagcattt ttcaaacgat cgataacgat agatccttca aatcctgcat tgtgagcaat
     1201 ttgacgaacg ggttcttcca aagcacggag aacaatatta cgtcctgttg cttcatctcc
     1261 tgtcaattcc aaggtagcaa cagctggaat cacattggca agagctgttc caccacctgc
     1321 aacaatacct tcttcaacag ctgcacgagt agcgttgagg gcatcttcaa tgcggagttt
     1381 catttctttc aactcagttt cagttgcggc tccgacctta ataaccgcta caccacctga
     1441 caatttggcc aagcgttctt gcaatttttc acggtcaaat tcagaagttg tagtttcgat
     1501 ttgagacttg ataaccgcaa cacggtgaga aatcgcttca ggatttcctg caccttctac
     1561 aataaccgtg ctatctttgt ccacggtcac tctcgctgct tgaccaagag cttcaattgt
     1621 cgcatctttc aactcaagac caaggtcttc tgtgataact gttccgcctg ttaagatggc
     1681 gatatcttca agcatggctt tgcgacggtc accaaaacca ggtgccttga ctgctactac
     1741 gttgaaggtt ccacgaatct tgttcaaaac aagagttgga agagcctcgc catccacatc
     1801 atccgcaata atcaagagtg gacgattgct ttggagaatg ctttccaaaa gtggcaagat
     1861 ttcttggata ttagaaattt tcttgtctgt aatcaaaatg tacggatttt caaggtcagc
     1921 caccattttt tcgctatcag tcaccatgta ctgtgaaagg taaccacggt caaactgcat
     1981 tccttctacg acttcaagct ctgtttccat accacgtgac tcttcgatgg tgatgacacc
     2041 gtctttgcca actttttcca ttgcttcaga gatgtactca ccaacttttt cagaacgaga
     2101 agatacggct gcaacttgag cgatagcttc tttattggca acagggatgg cgttgttttt
     2161 caaagcttca actgctgcgg caactgccgt ttcaatccca cgacgaatac cgattggatt
     2221 tgcacctgct gtgacgtttt tgattccttc acggacgatt gcttgggtca aaacagttgc
     2281 agttgtagtt ccatcacctg cgatatcatt ggtttttgaa gctacttctg ataccaattt
     2341 ggcacccata ttttcaaaat ggtcttccaa ttcgatttct ttggcaatgg tcacaccgtc
     2401 attggtaatc aaaggtgaac cgaatgactt ttcaagaacg acattgcgac cttttggtcc
     2461 caaggttact ttaacagtgt ctgcaaggat atcgacacca cgaaccatgg ctgaacgggc
     2521 atctgatgaa aatttaattt cttttgacat acttactttc tccttctatt cctcaatgat
     2581 tgccaaaatg ttagcttcgc ctacgatgat gtacttttca tcgccatctt tgacatcaag
     2641 acctgcgtgg gcttcaacta agacacgatc tccagtttta acacttggag caaccaagtc
     2701 accgttcaag gtacgaacac cttgtccagt agccacaact tgagctgctt tggttttttc
     2761 ttgggctgag cctgcaagga caaagccccc aacggtttgt tctttttctt ctatttttaa
     2821 gaccacacgg tcccctaatg gtttcaacat ctgatttcct ccataatgag atagatatta
     2881 ttagcactct ttgaacttga gtgctaattc ataattctat tgtatcactt ggtcagaaat
     2941 agtcaagaaa aaagtctgac tttctcaaga taaaaagcct gagaccaact cagacttttt
     3001 aatgtttata ctcaatgaaa atcaaagagc aaactaggaa gctagccgca ggctgctcaa
     3061 agcattgctt tgatgttgtg gataaagcta acgtggtttg aagagatttt cgaagagtat
     3121 taaaatggca attcttcctc ttccaagacc aaatctgcca aatcttggcc tgcattattt
     3181 tcacgcatgg cacgttgagc acgactttct aagagttgga atcctgtcac aaggacttcg
     3241 gttacatagt tcatctgacc atttttctca aagcgacggg tacgcaattc tccatcaacg
     3301 gaaatgagac tacctttggt tgcgtagctt gccaaagttt ctgctagcct gccccatagg
     3361 accatattga caaaatcagc ttcacgttca ccgttttggt ctttgtaacg acggttgaca
     3421 gcgatagttg ctcgcgctac cgacttgtca ttgttggttt tgtgcaattc tggtgtagac
     3481 gttaaacgcc caatcaagat aactttatta tacatatttt cttcctccta cttatctatt
     3541 cgtaggaaat caaaaaaagt tacagaaatt tgtaactttt cgaggaaatt tcttattttt
     3601 tatgaatcat gaaacctgtc gcctgttgat tggccataat ggtcatatct gtaatctgaa
     3661 cacggcgagg ctgactggtc acatagacta ctgtgtctgc aatatcctga gcttgcaagg
     3721 cttctattcc ttggtaaacg gacgcagccc gctctttatc accatgaaaa cgaacagttg
     3781 agaaatctgt ttcgacaatc ccaggctgaa tggttgtcac cttgatatcc gttgcgatgg
     3841 tatcaattcg cagtccatcc gaaaaggtct taaccgctgc cttggtagct gagtaaacag
     3901 cggcaccagc ataggcgtag attcctgcgg ttgaccccat attgataacg tgaccttgat
     3961 tgacttttat cattgctggc aagaaacagc gagtaaccgc catcagacct ttaacattgg
     4021 tatccaacat ggtcaacata tccaactcct catagtcttg atagggagcc aagtcaagag
     4081 ccagtccggc gttgttgacc aagatatcaa tcctccctat cgtttctaga atatcagagc
     4141 aaacagtctt caccatgacc atatccgtca catctagtgg aaaagtccag acagtttgat
     4201 ttggaaagct tactgcaaac tccgacttga ggatttctaa tctgtctatc cgtcgtcctg
     4261 ttagaacgac atcctcaccc tgctccagat aagcacgcgc aatcgcttca ccgattcctg
     4321 aggttgctcc cgtaatcact acattttttg ccatcttatt tccttctatc agatgctgac
     4381 aagttcctag gcagtccagt gtttcgctgg gtcgaacggt gttccgacaa cttggtcttc
     4441 tgataattca agcaccccac gtttttgcgg agcatttggc agatgcaatt cacgaggact
     4501 gcacatcata ccaaaactct tttcaccacg aagttcacct gggaaaatga gattcccttt
     4561 tggcatcata gctccaggaa gcgcgacaat ggttttcaac cccacacgcg cattgggagc
     4621 tcctgcaacg atttgtacag tcttatcact tgcgactgca acttggcaga tgttgaggtg
     4681 gtcactatct ggatgggcta ccatctcaac aatttcaccg acaacaaact taggttcctt
     4741 atcattaaca atttcttctg taaaaccttc cgcctgcaac tcttggttca aacgagcgac
     4801 ttgctcatct gtcaaaaaga cttgaccgcg ctctgcaatt tcaaataaac ttgaaacttc
     4861 gaaaatattc caagccactg tttccccatt atctttgaga aaaacacggg ctaccttgcc
     4921 tttgcgctcc acatccagtt tggcatctcc gctatttttc acgatgacca taaggacatc
     4981 accgacatat tctttgttat acgtaaaaat cattgtttcc tttttctcct atttcaatcc
     5041 tgctaaaaaa tcattgattt gttgcttgct tttacggtcg cgattgacaa aacgaccgat
     5101 ttccttgtcc ttttctagaa caacaaggct aggaattccg taaacatccc agagtttggc
     5161 caaatccata tactgatctc ggtccattcg aataaaggtg aactctggat tggtctcctc
     5221 aatctctggt aaggcaggat aaatataacg acaatcgcca caccagtctg ccacaaaaag
     5281 gaagaccttc ttgcccgctt tttccactaa agatgctaat tcttctaaac ttgctggctg
     5341 tatcataaga cttcctcctc atagactagg tcttcatttt catagataaa ggtataatga
     5401 cggccatcct caaaaatgac gccaccaacc aagctctcca gactgctttc gtaaacttga
     5461 acatagaggg tcgcaatttc ccccatgtcg gaaaaaaggt ctcgcacaat ctctgtcaac
     5521 tcttcctgag tcttcatgag cttacggtca tctgcaactt ttttcgtagc aagagcaagg
     5581 cttccgatac ctagcagagc caagcctgcc atccacattt ttttagcttt cataccattc
     5641 attttaacac aaaaaaggct tcaggacaaa tgaggaagca gcagaaaagc aagtaaaaag
     5701 cctcttcctt taaggaaaaa gacttcttat actaagagca aactaggaag ctagccgcag
     5761 gctgctcaaa acacggtttt gaggttgtag ataaggcgac gttgacgtgg tttgaagaga
     5821 ttttcaaaga gtattattct tattgccagg cacctaagtt gccaacgtag taactatcag
     5881 gtgtgtaggt attgcgaagc atcttacctg atgaagccag ataatactac ttgccattgt
     5941 ctttgaccca atcattcgca atcatggaac cagaagaact tacataatac cattctccct
     6001 tgtcataaac ccaagtactg actttcatgg ttcctgagca attaaaggca aaaaaactgt
     6061 ccaatatcat tcgtttttta aaagcatttg acactacatc atctacgctg tagtacatag
     6121 atacatcaaa cgtaaaagcc aaaatatcga aaaatataca acttttaaag gcagcatttc
     6181 attttttaaa gtctaacaac catagaactg aaatcagagc aaggcatcaa tttcctactc
     6241 ttagtacgtc tattttgatg gttgaagtga agattttttt atttaacgta gacaatttcc
     6301 aacttcccaa aagccacatc tccacggata gtcaaggttt tgctggttgg ggctacagga
     6361 gcgtcagcct tagctgcacc aaaggaggtt tctactttca aatctacacg ccagtgttgt
     6421 ggaacataga cggttgcatt cccgaaggcc acttcaatat ttaaagttgc aaaatcacct
     6481 agcatctctg cattatcata gtagatttta gcatccccaa aagcgacttc cacttggtca
     6541 tctacgaaat cttgatcttg cttatagaag gtcccgctac caaaagcgac ttccttatcc
     6601 gtgacgactg tttttttccc attgtaccac catttttttt cattccagaa cttacttgaa
     6661 tgcgtcagat aaccaacacc aagtaccacc aagatgctag cccaaataag agaatgattg
     6721 gtaactggta acaagtcata agcgtaattt gcaatgatga gcgccagtaa acctgtaaaa
     6781 actgccgaag tgagatgacg tctaaggatg gactcaatgg acttataagc aaaaaaaaca
     6841 atacctagta aaggccatat tttaccatcc aaagaaggaa ttccaaaatt cccttgcagc
     6901 aagatccaag ctgctaaaac caacaaaaca ataccaaatg ctttcttttt catgtttttt
     6961 acctcatgtt tcttagattt tcttttagga gggattggta atgccgtgag acatgaacct
     7021 ccttgtgcgt ctgataaaag gaaatggtgc ccgttcctga aaaggacttg tccactgagt
     7081 aaatctgacg gatgtttgcg atcgttgact tggaaactcg attaaaatag cgaggcaaga
     7141 tagactccaa ctcatagagc ttgaggcgaa cctcataagc ttccttctgg ttatgagcgt
     7201 agatcttgct cccttctgtt tcaaagaaga gaatttctga caggtctaga taatattcac
     7261 ctgtcccctt gtaaaaagtc aacctcggag cctttgactc ttgcaagagc cgttgcaagt
     7321 ctgcaatttc atctgtcaaa gtgggggtct tgatgacaat ttcagtctcc tctaaattgc
     7381 cgtcaatctc aattcgtaac ttcatcacat ctcctttctg actctactat atcaaaacta
     7441 agttaggaat acaagggcaa aaaagcaagt ggtcaatttg aagcagtaag tggttgtagg
     7501 actagcttaa cttacatgta agaataggaa gaaaaggaat ccccacacca aaccacaagc
     7561 atagccaacc aagacatcct tgggataatg aacaccccct aaaaccctca ccaaccccag
     7621 caaagctgaa aagatcaagc atactaagcc aataggtaga ctagcatgca gacaggccat
     7681 ggagatgata gttgccgaaa agacatggcg actgggcatc gaccgtcccg aactatcctt
     7741 atcaagaagg ggacagatat cccaagtttc ataaggtctc gggtgattga ttttcttacg
     7801 aaacagggac aaggccacaa aacctgatgc aggaataagc acacaaaccc caacttgctg
     7861 accaagtcct agttgcaggt aagtggtgac tagcaagatc agatagacca ggggcatggc
     7921 gactgtcatc aaacgattga aactgcgtaa taggaataaa agaattggac gactagttag
     7981 tctggagctg atatttcgat accattcttg ataatttttc atttatgctt ctcatacttt
     8041 tctagaactt gttgagatgt tttccccttg gcaagccaat ccactagctt gtccaagcga
     8101 cgtatttcct gcatcagagg atcatcaatt tcctcaatac ggacactgca aatcttccct
     8161 gtaattgcaa ttcgttcagg gacataatat ggtgcttgtc taaaaaaatc tccgtaagta
     8221 acatccctat ccagacaagc aagaacatca ctcacttcat accctgtcaa ccaactggtc
     8281 acttggtgaa ctgactctgc ctttccaccc tttcgctcaa cttttgcaat caaggctaag
     8341 tagacagcag caaatttcat attgtataat ttctgactca tacttctctc tttctatgtg
     8401 tactagtata aataagaaaa agaaggccgt caagccttct tttgatttat tcttctgctt
     8461 catcttctgt aaattgacta ttgtacaagt cagcgtagaa gccaccttgc gccatcagtt
     8521 cctcatagtt gccttgctca atgatatttc catctttcat gaccaagatc aagtctgcat
     8581 ttcggatggt tgacaagcgg tgggcaatga caaaggatgt gcgtccttcc atcaaacggt
     8641 ccatggcttt ttggatcaat tcctctgtcc gtgtgtcaac agaagaagtc gcctcatcca
     8701 aaatcaaaag cggtgcatcc ttaagaaggg cacgagcaat agtcaatagt tgtttttgtc
     8761 ctacagacaa ggtcacggtg tcatccaaga tggtatcata gccatctggc aaggtcataa
     8821 taaagtggtg aattcccaca gccttactag cttccatcat tcgttcatca ctaatcccta
     8881 tttgattata gatgagattg tctcgaatag ttccttcaaa gagccaggta tcctgcaaga
     8941 ccattgaaaa ggcatcatgc acttctgaac gcgtcatagc cttggtatcc acaccatcaa
     9001 tgtgaatact tcccttatca atctcataga atttcatcaa aagattgaca atggttgtct
     9061 taccagtccc agtcggccca acaatggcaa ccttttgacc agcatgaact gtcgcagaga
     9121 agtcatagtc ttgaacattg acaccgtcca ccagaatttc tcctgctgac acgtcgtaga
     9181 aacgtggaat cagattgacc agagttgatt taccagaacc tgttgaccca ataaaggcca
     9241 ctgtttgacc agtttctgct ttaaagctaa catgttcaat aactgcctcc gaatttgccg
     9301 catagcggaa ggtcacatcc ttaaactcga cctgaccttt gaagttttca tcagtcagct
     9361 gcacttgaac agggttttgg atagaagaat gcaaatctaa aacttgatta atccgcttag
     9421 cagagaccat agtttgggga agaacgatga agagtgctcc catgagaagg aagtccatga
     9481 caacctacat ggcataagac atgaaaacaa tcatgtcact aaagagaggc agacgcgcta
     9541 tcggagcagc gtcgttaatc acataggccc caatccagta aatcgccaca ctcaaaccac
     9601 ttgaaatccc catcatgata ggattcaaaa tagccataag acggttgaca aacaaattta
     9661 aacgggtcaa ttcatcattt actgctgcaa atttttcatt ttgataatcc tctgcattgt
     9721 aggcacgaac gacacgaata cctgttaaac tctcacgagt gatactgttc agtttatctg
     9781 tcagcccctg aatcaaggac tgttttggaa aggctagcgt catcaaaacg gtcgtcatca
     9841 ggacgttgac aatcactgcc acaagtacgg cccagagcca gtattctgaa tgacctaaaa
     9901 tcttcccaat agcccagata gccataattg gaccacacgt taccacttgc aagtccatag
     9961 taatcaacat ttgaacttga gtaatgtcat tggtagtacg catcaagagg ctaggaattg
    10021 aaaatttctt aatctctgtc tgcgagtaat ccaaaactcg gttaaaaata tcacttctca
    10081 gcctactagt ataagaagcc gccactcggg atgcaaaaaa tccaactgca actacggaca
    10141 agaaggcaag aaagggcatt cccatcatca tgcttgccgg ctgccacaac tcatctaaat
    10201 tagttccttg actacctagc aaatccgtaa ttttcgagat ataggtcggc acttccaact
    10261 ctagatagac cgaaaagcaa gtaaagagaa tggctagtaa aatcatcccc cattcttttc
    10321 tactaattct tttgactaat ttctttattc tctcctccta ttcccttgat atttgcctgt
    10381 agttgaccga gaactttctc aaaaatcagt aattcatctt catcaatgtc ttccatcaac
    10441 tgcttgtcta tgcgttcaaa aaaagcctta acctgttgca tctgagaacg tgctttgtcc
    10501 gtcagacgaa caaacttagc ccgcttatca acaggactcg cctccaattc caccaaacca
    10561 ttttgcacta tacgcttaac cagattacta gcaacagact tggtaatatt gagttcctgc
    10621 tcgatatctt taatcaagac caagtcttgg tttttctcgc gattatccaa aaaatgcaca
    10681 acctgacctt gcggcccacc cataaattca atgccgcaac gtttggcttc cttttgcacc
    10741 atcaggtgaa tttgatgacc aaaacgctta aagactaaca tcggtttatc cataatctcc
    10801 cccttctaaa taaaaatagt tctctggaga ataattaaat ttctatgaga actattttct
    10861 tgattaaaaa aatcccaagt gattttctca cttaggatca tgttctatag gttaaattaa
    10921 aacccatcta cgttcgtata aatcttttgg acgtcttcgt cgtcttcaag aacgctgtaa
    10981 agtttttcaa aggtttcaag gtcttcgcct gacaattcca cttctgactg aggaatcatt
    11041 tccaattcag tcacttggaa ctcttcaata ccagactcac ggagggcaac gatagccttg
    11101 tgaaggtcag ttggcgctgt gtaaactgtg attgtacctt cttgtgcttc tacgtcatcc
    11161 acatctacat ccgcttcgag caattgctca aagactgcgt ctgcatcttc acctccaaat
    11221 acaataacac ctttgttgtc aaagaggtaa gaaacagaac ctgaagcgcc catgtttccg
    11281 ccgtttttac caaaggctgc acggacattg gctgctgtac ggttaacgtt agaagtcaaa
    11341 gtatccacaa ttagcataga gccatttggc ccaaaacctt cgtaacgtcc ttctgtaaag
    11401 gtttcgtctg tgtttccttt ggctttatca atcgctttat cgataatgtg ttttggcact
    11461 tgggcttgtt tagcacggtc gataacgaat ttcaaagctg agtttgattc tggatctgga
    11521 tcaccttttt tagctgctac atagatttct acaccaaatt ttgcatatac tttagagtta
    11581 gctccatctt tagccgtttt cttggctacg atattggccc atttacgtcc cattaggaat
    11641 ctcctttttt cacattttaa tctttcttat tataacacaa gtttttttga ttttcactag
    11701 aggaaatgga ttttattagc aaatcaagct aggatagcac tttacctgct aagatggtct
    11761 tgcctttcta tctttatcaa cagacactca tccacattca aaaaacaaac tagaccatta
    11821 tctgcaaata gaaagtttca gccaagtttg acaaagtcag ctcaaattac tgtttgaagt
    11881 ttgtagatat aagcgacaaa aacaatcata ctgcaccttt tgttgacagt ctactccaga
    11941 catatcatag ttcaagtaaa tactttgaaa ttcaacagtt cttataggcg ctattgtatt
    12001 ctaagaaatc aatagaagag tttctaagca aacctctaat actcaataaa aatcaaagag
    12061 caaactagaa agctagcctc aggttgctca aaacactgtt ttgaggttgc ggatggggct
    12121 gacatggttt gaagagattt tcgaagagta taatttacgt gttcccaaga tggagaagtt
    12181 agactagtac actggcactt ctaaaccatt gctagcaatt gatttgttca tatttaattt
    12241 cattttttcc ataaatgggt attagatata aacagcaaaa tatttccgat acgtgtcgtt
    12301 cttgaatttc caatcatcta aaacaagtaa aggataatca atcccctgta tatcaaagaa
    12361 ttggctaccc tttttacttt tttacacatt ctgtttgata gattcatttt aacatcacga
    12421 gcatactcca atggaaatcg ctaggcaaga gataaacttt cagatatccg cagagagatc
    12481 atcgcctctt tttgtcgcaa gcattctcct ctcctagtca ttttctacct tatcttctac
    12541 ctgaggatag agagttgttc cccaaataga aatcgtccgc ttacgcacta gtggcaaatc
    12601 ggttttttca taaaccgtac gccaccattc ccaggcaagc ccggtacact ctctaatttt
    12661 gacagagaga ttacgaacat tcccttttaa aggaatacta gtggtaaagt gagccgttaa
    12721 atcctgccca tttctgtccc aagccttagg agtcaagact tccttacctt gatgatcata
    12781 ggataattca ttccaagtaa tataatattg ggcaacatag gcaccactat gatccagcag
    12841 taaatctccg tttctgtaag ctgtaacctt agtctcaaca tagtctgtac tattttgaaa
    12901 ggtcgcaact acattgtcac gtaaaaaaga agttgtatag gaaatcggca agcctggatg
    12961 atctgctgta aagcgactgc cttcttgaat caagtcctct accatatcca ccttgcctgt
    13021 tacaactcgg gcacccgaac ttgggtcgcc ccctaaaata accgccttca cttctgtatt
    13081 gtccaaaatc tgcttccact ctgtctgagg agctaccttg actcctttta tcaaagcttc
    13141 aaaagcagcc tctacttcat cactcttact cgtggtttcc aacttgagat agacttggcg
    13201 cccataagca acactcgaaa tatagaccaa aggacgctct gcagaaattc ctctctgttt
    13261 taaatcctct accgttacag tatcttgaaa cacatctcct ggatttttaa cagcgtctac
    13321 gctgactgta taataaatct gcttaaaatt aacaatctga atctgctttt cgcctgaatg
    13381 gacagagtta aaatcaatat caagagaatt ccctgtcttt tcaaagtcag aaccaaactt
    13441 gaccttgagt tgttccatgc tgtgagccgt gattttttca tactgcattc tagctgggac
    13501 attattgacc tgaccataat cttgatgcca cttagccaac aaatcgttta ccgctccgcg
    13561 aacacttgaa ttgctggggt cttccacttg gagaaagcta tcgctacttg ccaaaccagg
    13621 caaatcaata ctataagtca tcggagcacg atcgaccgca agaagagtgg gattattctc
    13681 taacaaggtc tcatccacta cgagaagtgc tccaggatag aggcgactgt cgttggtagc
    13741 tgttacagaa atatcacttg tatttgtcga caagctccgc ttctttcttt cgataacaac
    13801 aaactcatcg ggtagctgat taccctcttt gatgaaacga ttttcaatac tttctccctg
    13861 atgggtcaag agtttctttt tatcgtaatt catagctagt ataaagtcat ttactgcttt
    13921 atttgccatc ttctacctcc taataagttc ctggattgag ttgcataaac tcagacttgt
    13981 tcagcgaaat cagccgtggt tggactaagt gatccaaaat ttcctcgtac aattcttctg
    14041 agacattgcg tcgccgtctg gctagataag aagtcggaat aaccgtatta tccaacataa
    14101 ataccttatc taagtcaatc aaggttggtc ttgtaaaagg attacgagct agatccggct
    14161 cttctatcat aaagttcttg accaaacgtc tggtcaagag agctggtttg aaggtctgat
    14221 ttttaaccaa ctctttgttt ttagtcatgc tgttgtcaat acagatatac atatgattct
    14281 tcacagccaa atcgctacta atagtcggaa aaggcaaata aagaactaca acatctcctc
    14341 tcttaatcaa gcaagagcac ccccttttct cctaatgtaa catagacagg attgaccaag
    14401 tcttctgatt gactcagaat ttccaaagtt tgagtttggc gcgctgtcaa tttagtagca
    14461 tcttgtctct tcaatacaaa atgcttgtca ccaataacct tgacaatata atccttctcc
    14521 aaagctgact ggtaaatcca catcagatgt tgtctgtcct gagaactcaa gagagaagga
    14581 ttttcaagcc tcccgatagt ctgataaaaa tcaaaaacag gagctaactc ctgccaatct
    14641 gattggctag ttgtcaaggc tagaaaaagg gctttgcgag ctgatacttc ttggttagcc
    14701 ttgagagtta ctttcccctc caagtttttt agaaatcggg aaactccaga aagcaaattt
    14761 ttctctaact gcgagaaata aaaacctttc gttcccagac ataagtcttt catgtcgctt
    14821 tctctagcaa ataagagctc aaacatttga tagtaaaaga aaaatatctg gcactgggtc
    14881 gcgctcatct tttccttatc ggcttctttt tttaaccaga gcaagggcga caggtagctg
    14941 gattgagaca tttcctctac ctcctactct tttttaactg gagcatctgc actagctgcc
    15001 acttcttttg actggatact ttcccactgg ttaatctcct ctgagataag accttcgcat
    15061 gtcttgacaa atagggcaaa agccttggtc tttcctgcat atttctccgt ttggcattga
    15121 tagaggaatt tttctttctc caggagttgc gcagtttttt ggtaagaaat ccaattttcc
    15181 tttgcattat acaaattgat aatcccctca cacagcaagc cgagactgga taaggcaacc
    15241 gaaatcaaac ggtagcgatc acctggcata ggaatagcac aaaagacagc tatgaggaaa
    15301 cctgccacga tttctgttat ttttaatacc ttatagcgcc tacgatgttg aacgcttttc
    15361 tttaaaaaat gagctatctg tacgtctaat cgctctgtca ggtacatttc ttctggcgtc
    15421 atattcgtaa ctcctttcat ttactttgat aatcatagta taacatattt gaatatcaaa
    15481 gtatagtaaa aactggacta taacttatta gacttcctgc gaaacaaaac atggtatagt
    15541 agttctatga attatgaagc aagtaaacaa ctaaccgatg cacgatttaa gcgtcttgtt
    15601 ggtgttcagc gcaccacttt tgaagagatg ttagctgtat taaaaacagc ttatcaaaaa
    15661 agtcggacaa gctggtaaaa tcttggctga cagtggttat caagggccca tgaagatata
    15721 tcctcaagca caaactccac gtaaatccag caaactcaag tcgctaatag ctgaagataa
    15781 agcctataac catgcgctat ctaaggagag aagcaaggtt gagaacatct ttgccaaagt
    15841 agaaacgttt aaaatgtttt caacaaccta tcgaaatcat cgtaaacgct tcggattacg
    15901 aatgaatttg attgctggta ttatcaatca tgaactagga ttctagtttt gcaggaagtc
    15961 tattatatat atgcacagac atatgaaatc tttttgatac agccttcttt ttacatagag
    16021 agacagcagt tatgatatat ttatccatac acaacatctt ctgtaccaat actacaatat
    16081 tggtacattt tgattacaca atcgtttcaa aaaatatgat ggaagcctga gtattttata
    16141 gtaattccct tcctatcaac tagatcaaac agttgagtgt tgaatgttga aacaccctag
    16201 cctcagcaaa ggactatgat cagcatttcc tagccaatag gacgctccaa atcacctatt
    16261 ctaagtccat cactagcttg gtcagtaaga tacagactgc aaggcgagac tgacgtggtt
    16321 gatgagattt ttgaagagta ttacaatcat agattcacca gtagacttga ccgcagattt
    16381 aactatagta gattgaaact agaatagtac acctctgctt ctaaaacatt gttagaaatc
    16441 gatttgactg tcctgatcaa tttgtcctgt tctcgtttca ttttactata ctaaaaaacc
    16501 acctgaaaca aatcaagtga ctagaggata aaaacaaaga ttgggcaaaa agttttgaaa
    16561 agcaaaaaac gcataatatc aggtcttgaa aaaccttgat actatacgtt tgattgtggg
    16621 aagattgact tcattttctc ctgaaattga agttttttct agtttccaga tatagacgaa
    16681 ttcaatatag ccatctcgct acaattattt taaccatcga gaaaattttt cagaatttca
    16741 aaaatttcat tttgatccaa tgaaatgtca tcattttgaa gaatagttga cttcacaggc
    16801 tctaattcta tcacaaagcg gtgcatgtct gcttttaccg cactacttga tgttatagcc
    16861 gataactttt catctcctag caaggaagct gtcaaacggg taaggtcatt caaatgcttt
    16921 ttaatggact tacttaaatc ttgagcacct gtagcagagc gctctctcat atccagccaa
    16981 gctttagcct taaaaataat taatactcaa tgaaaatcaa agagcaaact aggaagctag
    17041 ctgcaggctg ctcaaaacac tgtttt
//
LOCUS       DAVKJJ010000041        16506 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000041 DAVKJJ010000000
VERSION     DAVKJJ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16506
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.041"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            <1..220
                     /locus_tag="VW144_001497"
     CDS             <1..220
                     /locus_tag="VW144_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600924.1"
                     /translation="IKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGY
                     LFSVLLLFSNFSVPFIKAKEYELFKNSK"
     gene            1353..1592
                     /locus_tag="VW144_001498"
     CDS             1353..1592
                     /locus_tag="VW144_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600925.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLYKITRDYASQTKPAIQAGVFILEDFRNILCTMM"
     gene            1577..1855
                     /locus_tag="VW144_001499"
     CDS             1577..1855
                     /locus_tag="VW144_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600926.1"
                     /translation="MYNDVIERISLYEFIGDIFYSKIISCCIVARDLSKNTMKLDVIF
                     FEDKNKRSAVLGLRRDKSGVFKSVTLHFTSAKKYAKVRKTDVKEMKWL"
     gene            2141..4057
                     /gene="pspA"
                     /locus_tag="VW144_001500"
     CDS             2141..4057
                     /gene="pspA"
                     /locus_tag="VW144_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein A"
                     /protein_id="HEU9600927.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEEAPQVVEKSSLE
                     KKYEEAKAKADTAKKDYETAKKKAEDAQKKYDEDQKKTEDKAKAVKKVDEELQKANLE
                     LQKAHVKYQKAQRELSESPDRRKSAARKKLADALSYIGEVELKQKEAEANFNTEQAKV
                     IPKETELAVTKQKAEEAKKEAEVAKKKFDKAAQEVEVAKKEVEAKELEIEKLQDEIST
                     LEQEVATAQHQVDNLKKLLAGVDPDDTEAIEAKLKKGEAELNAKQAELAKKQTELEKL
                     LDSLDPEGKTQDELDKEAAEAELDKKADELQNKVADLEKEISNLEILLGGADPEDDTA
                     ALQNKLATKKAEFEKTQKELDAALNELGPDGDEEETPAPAPAPKPEQPAPAPAPKPEQ
                     PAPAPAPKPEQPAPAPAPKPEQPTPAPKTGWKQENGMWYFYNTDGSMATGWLQNNGSW
                     YYLNSNGAMATGWLQYNGSWYYLNANGAMATGWAKVNGSWYYLNANGAMATGWLQYNG
                     SWYYLNASGAMATGWLQYNGSWYYLNASGAMATGWAKVNGSWYYLNANGAMATGWAKV
                     NGSWYYLNANGSMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGLGALAVNT
                     TVDGYTVNENGEWV"
     gene            4458..5579
                     /gene="mnmA"
                     /locus_tag="VW144_001501"
     CDS             4458..5579
                     /gene="mnmA"
                     /locus_tag="VW144_001501"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU9600928.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKALSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTRDMPEEFTLECTAKFRYRQPDSKVTVHVKGEKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            5720..6175
                     /locus_tag="VW144_001502"
     CDS             5720..6175
                     /locus_tag="VW144_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9600929.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVREELGVKAQAGQLAFVVENRFEQDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            6185..8098
                     /gene="mnmG"
                     /locus_tag="VW144_001503"
     CDS             6185..8098
                     /gene="mnmG"
                     /locus_tag="VW144_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEU9600930.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(8293..9972)
                     /locus_tag="VW144_001504"
     CDS             complement(8293..9972)
                     /locus_tag="VW144_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9600931.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(9974..10207)
                     /locus_tag="VW144_001505"
     CDS             complement(9974..10207)
                     /locus_tag="VW144_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEU9600932.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            10589..10810
                     /locus_tag="VW144_001506"
     CDS             10589..10810
                     /locus_tag="VW144_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600933.1"
                     /translation="MTNQVLEENYLCDSLLAFSPPRDLCRLVCILLWIKCLDALLMLG
                     FFVWGCRDSRFTTRKFHPIYQDEVVFAFF"
     gene            complement(11023..11175)
                     /gene="cibB"
                     /locus_tag="VW144_001507"
     CDS             complement(11023..11175)
                     /gene="cibB"
                     /locus_tag="VW144_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEU9600934.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(11177..11362)
                     /gene="cibA"
                     /locus_tag="VW144_001508"
     CDS             complement(11177..11362)
                     /gene="cibA"
                     /locus_tag="VW144_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEU9600935.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            11540..12223
                     /gene="tsaB"
                     /locus_tag="VW144_001509"
     CDS             11540..12223
                     /gene="tsaB"
                     /locus_tag="VW144_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU9600936.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAYTLNIELVGMSSLLALVPH
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLSFEEVLEKVKGTSQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            12220..12657
                     /gene="rimI"
                     /locus_tag="VW144_001510"
     CDS             12220..12657
                     /gene="rimI"
                     /locus_tag="VW144_001510"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU9600937.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEVEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            12647..13657
                     /gene="tsaD"
                     /locus_tag="VW144_001511"
     CDS             12647..13657
                     /gene="tsaD"
                     /locus_tag="VW144_001511"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU9600938.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLYHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWNLNAKPSLAFDT
                     ME"
     gene            complement(13697..15003)
                     /locus_tag="VW144_001512"
                     /pseudo
     CDS             complement(13697..15003)
                     /locus_tag="VW144_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(15278..15629)
                     /locus_tag="VW144_001513"
                     /pseudo
     CDS             complement(15278..15629)
                     /locus_tag="VW144_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(15736..16029)
                     /locus_tag="VW144_001514"
                     /pseudo
     CDS             complement(15736..16029)
                     /locus_tag="VW144_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            16272..>16506
                     /locus_tag="VW144_001515"
     CDS             16272..>16506
                     /locus_tag="VW144_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU9600939.1"
                     /translation="MQYSCGKININIPDGYGDIKDIVFSAHIIVRYNNGHCGGIDPHI
                     IGLCKKQIRRMSLYPILIIVSRDSKVIDDYKNLD"
BASE COUNT         4988 a         3071 c         3722 g         4725 t
ORIGIN      
        1 gataaaattt ttattcatca gattatttga cacaagtact ttaatgaaga ggagaatatt
       61 ttttcttttc gcattggatg ttatagctta cttattttta ggatatcttt tagtgattca
      121 aaaagcaggc tatttgtttt cggttttact tcttttttct aatttttcag ttccatttat
      181 caaagcaaag gaatacgaat tatttaaaaa tagcaagtaa tagaaacata ggttaaatct
      241 tagaacggct taaaatcaga tatgataaag ggaaatagtc ttatctaggt aactaagtgc
      301 tacaaacaca gtcgaggatc acttgttagg attacatttt aatcatgctt aataagaatg
      361 gagaattcct atggggattg tattactctg aagtttcgaa taccttagtt ctcgtgattt
      421 tgagctgttc tagctttaga atccttcaga atgtaaaatt ttaagcagtc aaagacttag
      481 aatagcctgg aaacatttat tttatagcaa ttctaggctt agaattgctc atatatatgt
      541 atttgagctg ctcgatagtt aggacagctc tattctgaga aatctgagct atcaatgctt
      601 cagaatagta tttcgcaaat ggtttttatt gaagaggtga ttgttttatg atagtctaaa
      661 ataaaactga tttaatggag taaatggaga atcgtcacat gaaacatttt tttgcaggaa
      721 ttggtgagat aaaacttgtt ggctacttat tgtatatatg tgtaggacta gcccctcttt
      781 ttcatatata tgtcataggt tcggaactga gttttgtgaa aatagtgtta tctattttgg
      841 gagttatatt tgtaagtatg ttaactattg ctcgaattta tcggactttt ctataaagaa
      901 tagtttggac ttgaactgta tctaaaaaca gaggttggtc gattatcagc ctctttcttg
      961 tcgttgaaaa gagtgagata tattgacttt tgaaaaaaat gtcataattg agttgggata
     1021 aacctaaaag taatatagta gattgaatct ggaatagtcc gttatgactg ttaaaatatt
     1081 tctagaaatt aatttgactt tcctaatcga tttgttcaca tcttgtttca atctactata
     1141 ctttctcgtt aaaccgttcg gcaattcatg gtgacatgga cacatagtag taagcacact
     1201 atgtggcttg gcagagctaa aaactgttcc cttgcgataa gcctaataag cacaacatag
     1261 ggagttagag aagccgactc taatcaacca ctttgggcag tagtgagaac tgccccgtgc
     1321 ttttttattt tgagaaaata tggagtttgt cgttgaaatt acttgattgt attttggact
     1381 atcaagaaag attcaatgga aaaacatgtc aagtatcaac gaattataag tatttagaga
     1441 ttttcaaagt gaatttttgc ttgactgatt tacatcattt atttgactta tacaaaatca
     1501 cacgagatta tgctagtcaa acaaaacctg ctattcaagc tggtgttttt attttagaag
     1561 attttagaaa tatcctatgt acaatgatgt aatcgaaagg atatctttat atgagtttat
     1621 cggtgatatt ttctattcta aaataataag ttgttgcatc gtagctaggg atttatctaa
     1681 aaatactatg aaattggacg tcatattttt tgaggataaa aataaaagat ccgcagtttt
     1741 aggtttacga agagacaaaa gcggagtatt taaatcagtt actctacatt ttacaagcgc
     1801 taagaaatat gctaaagttc gtaaaacaga tgtgaaagaa atgaaatggc tataaagatt
     1861 gtccgcaggc ttaagcttgc gttttttgtt gtttaacgaa attaaaaaac aagctctctc
     1921 atcggaaatg tttttatagg aatgaaggaa gatgatgcta tgtttaaaaa gagaatcgaa
     1981 aagggcaaaa gtagtgtctt tatctttttg gagtagaagc ttatgatata gaaatttgta
     2041 ataaaaatgt aatataaaac atttgacaaa tatttacggg gggagtatac ttaatataag
     2101 tatagtctaa aaatgactat cagaaaagag gtaaatttag atgaataaga aaaaaatgat
     2161 tttaacaagt ctagccagcg tcgctatctt aggggctggt tttgttacgt ctcagcctac
     2221 ttttgtaaga gcagaagaag ctccacaagt tgtcgaaaaa tcttcattag agaagaaata
     2281 tgaggaagca aaagcaaaag ctgatactgc caagaaagat tacgaaacgg ctaaaaagaa
     2341 agcagaagac gctcagaaga aatatgatga ggatcagaag aaaactgagg ataaggcaaa
     2401 agcggttaag aaagttgatg aagaacttca aaaagcaaat ttagaacttc aaaaagctca
     2461 tgtaaaatat caaaaagcac aaagagaact tagtgaaagt ccagacagaa gaaaatctgc
     2521 tgctagaaag aaattagctg atgcattatc ttatattgga gaggtagagt taaaacaaaa
     2581 agaggcggaa gcgaatttta atacggaaca agcaaaagta attcccaaag aaactgagtt
     2641 agctgttact aaacaaaaag cggaagaagc taaaaaagaa gcagaagtag ctaagaaaaa
     2701 atttgataag gcagctcaag aggtagaagt agcgaagaaa gaagtagagg ctaaggaact
     2761 tgaaattgaa aaacttcaag atgaaatttc tactttggaa caagaagttg ctactgctca
     2821 acatcaagta gacaatttga aaaaacttct tgctggtgtg gatcctgatg atacagaagc
     2881 tatagaagct aaattaaaaa aaggggaagc tgagctaaac gctaaacaag ctgagttagc
     2941 aaaaaaacaa acagaacttg aaaaacttct tgacagcctt gatcctgaag gtaagactca
     3001 ggatgaatta gataaagaag ctgctgaagc tgagttggat aaaaaagctg atgaacttca
     3061 aaataaagtt gctgatttag aaaaagaaat tagtaacctt gaaatattac ttggaggagc
     3121 tgatcctgaa gatgatactg ctgctcttca aaataaatta gctactaaaa aagctgaatt
     3181 tgaaaaaact caaaaagaat tagatgcagc tcttaatgag ttaggccctg atggagatga
     3241 agaagaaact ccagcgccgg ctcctgcacc aaaaccagag caaccagctc cagctcctgc
     3301 accaaaacca gagcaaccag ctccagctcc tgcaccaaaa ccagagcaac cagcgccggc
     3361 tcctgcacca aaaccagagc aaccaactcc tgcaccaaaa acaggctgga aacaagaaaa
     3421 cggtatgtgg tacttctaca atactgatgg ttcaatggct acaggatggc tacaaaacaa
     3481 cggttcatgg tactacctca acagcaatgg cgctatggct acaggatggc tccaatacaa
     3541 tggttcatgg tattacctaa acgctaacgg cgctatggca acaggttggg ctaaagtcaa
     3601 cggttcatgg tactacctca acgctaatgg tgctatggct acaggatggc tccaatacaa
     3661 tggttcatgg tactatctaa acgctagcgg cgctatggca acaggttggc tccaatacaa
     3721 tggttcatgg tactatctaa acgctagcgg cgctatggca acaggttggg ctaaagtcaa
     3781 cggttcatgg tactatctaa acgctaacgg cgctatggca acaggttggg ctaaagtcaa
     3841 cggttcatgg tactacctca acgctaacgg ttcaatggca acaggttggg tgaaagatgg
     3901 agatacctgg tactatcttg aagcatcagg tgctatgaaa gcaagccaat ggttcaaagt
     3961 atcagataaa tggtactatg tcaatggctt aggtgccctt gcagtcaaca caactgtaga
     4021 cgggtatact gtcaatgaaa acggtgaatg ggtttaagcc gattaaatta aatcatgtta
     4081 agaacatttg acattttaat tttgaaacaa agataaggtt cgattgaata gatttatgtt
     4141 cgtattcttt aggtacctgt cttatgattt caggaaatgt cattaaaaaa acgactcctt
     4201 ttctctaact tgaaaaatag attagagaaa atgggttgtt ttatttatta tagttatgtg
     4261 aatgaagata agaagaaggt atactcacat cattcacata atctgtatat tgactataag
     4321 ttttaaaaaa caatttttaa gctcttcctt gtcttctcta accaagcgtg ttataatgaa
     4381 tactgctcaa gcgaccttca atcgtgaagc acacacgacc ttcaatcgtg aataaacgaa
     4441 tagatgggag acttaccatg agtgataact ctaaaacacg tgttgttgtg gggatgagtg
     4501 gtggtgttga ttcgtcggtg acggctcttc ttctcaagga gcagggctac gatgtgatcg
     4561 gtatcttcat gaagaactgg gatgacacag atgaaaacgg cgtctgtacg gcgaccgaag
     4621 attacaagga tgtggttgcg gtggcagacc agattggcat tccctactac tctgtcaatt
     4681 ttgaaaaaga gtactgggac cgcgtttttg agtatttcct agcggaatac cgtgcgggac
     4741 gcacgccaaa tccggacgtt atgtgcaaca aggaaatcaa gttcaaggcc tttttggact
     4801 atgctatgac cttgggggca gactatgtag cgactgggca ttatgctcga gtggcgcgtg
     4861 atgaggacgg cactgttcac atgcttcgtg gtgtggacaa tggcaaggac cagacctatt
     4921 tcctcagcca actttcgcaa gaacaacttc aaaaaaccat gttcccacta ggacatttgg
     4981 aaaagcctga agtacgcaga ctagcagaag aagcaggcct ttcgactgct aagaagaaag
     5041 actcgacagg gatttgcttt atcggagaaa agaactttaa aaacttcctc agcaactacc
     5101 tgccagctca gcctggtcgc atgatgactg tggatggtcg cgatatgggc gagcatgcag
     5161 gtcttatgta ctatacaatc ggtcagcgtg gcggactcgg tatcggtggg caacacggcg
     5221 gtgacaatgc cccttggttc gttgtcggaa aagctctaag caagaatatt ctctatgtag
     5281 gccaaggttt ctaccatgat tcgctcatgt caaccagcct agaagctagc caagtccact
     5341 ttactcgtga tatgcccgag gagtttacgc tagaatgtac ggctaaattc cgttaccgtc
     5401 agcctgactc taaggtgaca gtacatgtca aaggagaaaa ggcagaggtc atctttgcgg
     5461 aaccacaacg cgcgattaca ccaggacagg cagttgtctt ttacgatggc gaagagtgtc
     5521 taggtggcgg tttaattgac aatgcttacc gcgatggaca agtttgtcag tacatttaga
     5581 ttgacaaatt ttctcaattt gctacaataa taacagcaat agaaatgatg gtcaaagctc
     5641 atggatgttg caggcttttt tgtcctgcac ttctttgtag ttttgactgt ttttgtgtcg
     5701 tttaagggaa aggacaagaa tgactcaaca agactttcgg acaaaagtag acaatacggt
     5761 ttttggtgtt cgggcgacag ccttgattgt ccaaaatcac aagcttctag ttaccaaaga
     5821 caagggcaag tattacacta ttggaggtgc gattcaagtc aatgaaagca cggaagacgc
     5881 ggtagtccgt gaagtgaggg aagaactggg tgttaaagct caagctgggc agctagcttt
     5941 tgtggttgaa aatcgttttg aacaagacgg cgtttcctat cacaatatcg agtttcatta
     6001 tctggtggat ttgcttgaag atgccccgtt gaccatgcag gaagatgaga aaaggcagcc
     6061 ctgtgagtgg attgacttgg ataagcttga ggatatccag ctagttccag cctttttaaa
     6121 aacggcccta ccagattggg aaggccaact aagacacatt catcttgaag aataggagag
     6181 aaacatgact tatcatttta ctgaagaata cgatattatt gtaattggtg cgggacacgc
     6241 tggggttgag gcttccttag ctgctagccg tatgggctgt aaggtcctgc ttgcgactat
     6301 taacattgaa atgctggctt tcatgccttg taatccctct atcggtggtt ctgctaaggg
     6361 gattgtcgta cgtgaagtcg atgccctcgg tggcgagatg gccaagacca ttgacaagac
     6421 ttacatccag atgaagatgc tcaacacagg gaaggggcca gctgtccgtg ctcttcgtgc
     6481 gcaggctgac aaggaacttt actctaagga gatgcgcaag acagttgaaa atcaagaaaa
     6541 tctgaccctt cgtcaaacca tgattgatga gattttggtg gaagatggca aggttgtcgg
     6601 tgtgcgtaca gccacccatc aagaatatgc tgctaaggct gttattgtga cgacagggac
     6661 tgctctccgt ggggaaatta tcatcggaga cctcaagtac tcatcaggtc ctaaccacag
     6721 cttggcttct attaacctag ctgacaatct taaggaactg ggtctcgaaa tcggtcgttt
     6781 caagacagga acccctccac gtgtcaaggc ctcttctatc aattacgatg tgacagaaat
     6841 tcagccagga gacgaagtgc ctaatcattt ctcatacact tcacgtgatg aggattatgt
     6901 caaagatcaa gtgccatgct ggttgaccta taccaatggt accagtcatg agattatcca
     6961 aaacaacctc caccgtgcgc ctatgtttac aggtgtggtc aagggagtgg ggcctcgtta
     7021 ctgtccgtcg attgaagaca agattgtgcg ctttgcggac aaggaacgtc accaactctt
     7081 ccttgagcca gaagggcgca atactgagga agtctatgtt caaggacttt caaccagtct
     7141 gcctgaggat gtccagcgtg acttggttca ttccatcaaa ggtctggaaa atgcagagat
     7201 gatgcggaca ggttatgcta ttgagtatga catggtcttg cctcatcagt tgcgtgcgac
     7261 tttggaaacc aagaaaatct caggtctctt cactgctggt cagacaaatg gaacatcagg
     7321 ttatgaagaa gctgctggcc aagggattat cgcgggtatc aatgcggctc tgaaaatcca
     7381 aggtaaacct gagttgattt tgaagcgcag tgacggctat atcggggtca tgatcgacga
     7441 cttggtgacc aagggaacca ttgaacctta ccgtctcttg accagtcgtg ctgaataccg
     7501 tctcattctt cgccatgaca atgccgatat gcgtttgaca gagatgggcc gtgagattgg
     7561 ccttgtggat gatgaacgct gggctcgttt tgaaatcaag aaaaatcaat ttgataatga
     7621 gatgaaacgc ctagacagta tcaaactcaa gccagtcaag gaaaccaatg ccaaggttga
     7681 ggagatgggc ttcaagccgt tgacagatgc ggtgacagcc aaggaattcc ttcgccgtcc
     7741 agaagtttct taccaagatg tggtggcctt catcggacca gctgcagaag acttggatga
     7801 caagattatc gaattgattg aaacagaaat caagtacgaa ggctatattt ccaaagccat
     7861 ggatcaggtt gccaagatga aacgtatgga agaaaaacgc attccagcca atatcgactg
     7921 ggatgacatc gattctattg cgacagaagc tcgtcagaag ttcaaactca tcaatccaga
     7981 aaccatcggc caagccagcc gtatttcggg agtaaaccca gcagatattt ctattttgat
     8041 ggtgtatctg gaaggtaaaa atcgtagtat ttctaaaact cttcaaaaat caaaatgata
     8101 cgtcgtcggc ttcttacgaa tgagttcaaa gcttggcttt gattcatctc cagcttccca
     8161 tagttccccg aactatggga gctaactcaa gttatgcatt ttcctagcct tctggtctga
     8221 ttttcattga gttttaatct caaaagaaag catagagaaa aacagctccg aggacggggc
     8281 tgttttcttg ctttattctt catctggtgt gaggatcatc gggatgatga tcggttcacg
     8341 ttcggtattt tcatagagga aggggcgaat agcgttgaca atggcaccat tgacagattg
     8401 cacgctagca tccttatttt tcagtgcgat acgaatggca ttgaagagga tacgctggct
     8461 ttggcgaatc aagtcgccag actctctcat gtagacaaag cctcggctga ggatgtctgg
     8521 accagataga atcatctgcg atttgaagtc aacagttgcg actgccagaa cgacaccgtc
     8581 ttcagataga tcgcgacgat ctttgaggac agctgcgcca atttcaccga tacgatttcc
     8641 atcgacatag atatcttggg cgttgaaatg acctgcgata cgagctgagt cagcagtaag
     8701 ggcaagcaca tcgccattgc tcatgataaa gatattgtcc ttctcaacac cagtatccac
     8761 tgctagtcca gcgtggactt tttgcatgcg gtattcaccg tggacaggca tgaagtattt
     8821 tggcttaatc aagcagagca tgagtttttg ctcttgctga ccaccgtgtc cagatgtatg
     8881 gatattgttc actttaccgt ggataacttc gacaccagct tcagaaatga tgttaatcag
     8941 cttgttgacg ctagtagtgt ttccagggat gggactagaa gagaagataa cggtatcacc
     9001 tggttgtaat tgtacttgac ggtgggttcc gttggcgata cgagagaggg ctgccatagg
     9061 ctcaccctga ctacctgtac agaggataag aacttctcct gcaggataat ctttgatttc
     9121 atttggctcg ataaaggttc ccttaggagc tttgatgtag ccaagatcga ttccgttgac
     9181 aatggccttt tccatagaac gaccaaagac cgcaatcttg cgtccagtct taacagcagc
     9241 ttctgttgcc tgctggagac ggaagatatt tgaggcaaag gatgcaaaga tgatacgtcc
     9301 ttcaatacct tggataatct tcataatgga ctgaccaacg actttttcag agttggtaaa
     9361 ggttggtact tccgcatttg tcgagtcaga caggagacag agcacgcctt cttcaccaag
     9421 cgcagccata cgatgcaagt ccgcaggttc tccaactgga gtaaagtcga acttaaagtc
     9481 acccgtacag acgattttcc cttgaggagt atgaatgaca atccccaaag gctctggaat
     9541 agagtgagtc gttctaaaga aagttgcctt gagattttta aaggtcaact cggtgttgtg
     9601 gttgatttcg taaagtttgg cgttgcgcaa gaggccgtgt tcttcgagtt tcccacggat
     9661 caaagccaag gcaagcggtc cagcataaat agggacattt gcttgcttga gtaggaacgg
     9721 aatcccacca atgtggtcct cgtgtccgtg tgtgattaaa acagccttga cgcggtcgat
     9781 attgtccacg atgtaagagt agtcaggaat gacatagtcg ataccaagca agtcatcttc
     9841 tgggaattta atcccagcat cgacgatgat aatctcgtct tggtattcaa ttccgtaagt
     9901 gtttttcccg atttctccta gaccaccgat ggcaaaaacg ccgacttctt caggtttaag
     9961 agtgtaggcc atattagaac tccgtaattt cgaaggcgcc agtttctttt tcgtaatcga
    10021 gcaatttgtc agacaagagt tcgatatatt cgatattgta ctctgggcga ttttcttcga
    10081 caagttggcg agcagtgata cggccctcaa gttctgagct ggcatcgatg tctaggtaaa
    10141 gcgcgcgtgt tgtttcacgg cgtgggctac gttcttttgt ttcttgataa aaaactttgt
    10201 aaatcatata gttcctttct atttacaggt cagcttattc caaactttta gcagatattt
    10261 gcttgatttc attccatttt gtgtctagat tgaccttgat tcgttacaat tatttcaatt
    10321 ataccacaaa atgaaaaatt agtaaaagac ggaaacgaga aagttagggg acgatttctt
    10381 cggtaagcgt tttcaaaaat aaagaaaatg tattatgttg ataacggaag tgaaatttat
    10441 tgcttgtcat aattccatat agattttaag tcaaggaggt tgccctatgt ataacttatt
    10501 tgcttttgct atcggagttt gtttgctagc ttttatcgtt tttgttttgc gtttatggag
    10561 gaaatcttct atttgccaaa ctggaacgat gacaaaccaa gttcttgagg aaaattacct
    10621 ttgtgactct ctactcgctt tttctcctcc tcgtgaccta tgtcgtttgg tttgtatttt
    10681 actttggatt aaatgcttag atgccctgct tatgctgggg ttttttgttt ggggatgtag
    10741 agatagtaga tttacgacac gaaaattcca ccccatctat caagatgagg tggtctttgc
    10801 atttttctaa aatgatatga caatggaatt ttcaaagctt attattttta tgggagtcat
    10861 tgttctgatc aaaaatagag ttgaagcata aaaataaaac aatacaaaca gataaatcga
    10921 atggtaaagg gatggtagta atgttcaaat cattgagaat gctgagcaaa ctgtaagaga
    10981 tgaagacaat aaaatttatc cttctaaaaa tgtgtttctt atctagcatg acttaccaaa
    11041 ctttttacga aggtcttttc caatggtata acctgccata aataaagcga taccaacacc
    11101 gacaccaaag ccacccttga tttcttgcaa ttccttatta gaaatttcac gataattgtt
    11161 caaatctttc atcatattaa tctcctccta aaaaataacc agctgccaaa ccagaaccta
    11221 taagtgctgt tccacctgca atagccttcc aagatactgc tactccaaag ataaccaagg
    11281 gagcgagacc gccatcaata tctgataatt cattttcaga taaggataaa aattgattgt
    11341 caagaatgtc aaaatttgtc attgaacttt cctccttttt tcttgttcac ttttatattc
    11401 ggaaaagaaa aggaaaagtg agcatttttt taaattattt ttagttaagt gagggcactc
    11461 ttgtctgggg gcaaagaaaa ttcccatgtg ccagcttttg tagtataata gtaagcagac
    11521 aaaaagatag gaatgtgtta tgaaagtatt agcttttgat acgtccagca aggctctttc
    11581 tctggctatt ttagaggata agcaggttct tgccgagacg acgattaata ttaagaaaaa
    11641 tcacagtatt actcttatgc ctgccatcga ttttttgatg gcaagtttgg attggacacc
    11701 caaggatttg gaccgaatcg tggtagctga agggccgggt agctatacag gcttgcgaat
    11761 tgcggtagca actgctaaga ccttagctta caccctgaac atcgagttgg ttggtatgtc
    11821 gagtctcttg gctctggtgc cccaccaaca agaaggtttg tttgtcccct tgatggatgc
    11881 gcgtcgcaat aatgtttatg caggatttta tgaaaatgcc aaacctgtca tggcagaagc
    11941 gcacctatct tttgaagagg tgctagaaaa agtcaagggt actagtcagg taacctttgt
    12001 cggagaagtt ggcccctttg ttgagcagat tcaaaaacac ttgccaagga ctgattacaa
    12061 agaaacattg cccaatgcag ctaatctagc tcttttggcc tgggacaagg aagcagactc
    12121 cttgcatgat tttgtgccga attacctcaa acgagtcgag gctgaggaaa actggctcaa
    12181 gaaccatacc gagtctggcg agtcttacat taaacgccta tgatagaaat caagcgaatt
    12241 caacaacagc ctgacctagc tcaagccatc tacgctgtta tggcagctgt ttacctagtc
    12301 agtccttgga ctctggagca aatccaagca gatctgtccc aagaccagac ttggtatgca
    12361 ttggcttatg atggggcaga agtgattgga tttctagctg tgcaggagaa tctttttgaa
    12421 gtagaagtcc tgcaaatcgc tgtcaaagga gcctatcagg gtcagggaat tgcgtcagcc
    12481 ttgtttgctc aattgccgac agacaaggaa attttcctcg aagtcagaca gtcaaatcaa
    12541 cgagcgcaag cattttacaa gaaagaaaag atggcagtta tcgctgagcg aaaggcctac
    12601 taccatgacc cagtcgagga cgccattatc atgaagagag aaatagatga aggatagata
    12661 tattttagca tttgagacat cctgtgatga gaccagtgtc gccgtcttga aaaacgacga
    12721 tgagctcttg tccaatgtca ttgctagtca aattgagagt cacaaacgtt ttggtggcgt
    12781 agtgcccgaa gtagccagtc gtcaccatgt cgaggtcatt acagcctgta tcgaggaggc
    12841 attggcagaa gcagggatta ccgaagagga cgtgacagct gttgcggtta cctacggacc
    12901 aggcttggtc ggagccttgc tagttggttt gtcagctgcc aaggcctttg cttgggctca
    12961 cggacttcca ctgattcctg ttaatcacat ggctgggcac ctcatggcag ctcagagtgt
    13021 ggagcctttg gagtttccct tgctagccct tttagtcagt ggtgggcaca cagagttggt
    13081 ctatgtttct gaggctggcg attacaagat tgttggggag acacgagacg atgcagttgg
    13141 ggaggcttat gacaaggtcg gtcgtgtcat gggcttgacc tatcctgcag gtcgtgagat
    13201 tgacgagctg gctcatcagg ggcaggatat ttatgatttc ccccgtgcca tgattaagga
    13261 agataatctg gagttctcct tctcaggttt gaaatctgcc tttatcaatc tttatcacaa
    13321 tgccgagcaa aagggagaaa gcctgtctac agaagatttg tgtgcttcct tccaagcagc
    13381 agttatggac attctcatgg caaaaaccaa gaaggctttg gagaaatatc ctgttaaaac
    13441 cctagttgtg gcaggtggtg tggcagccaa taaaggtctc agagaacgcc tagcagccga
    13501 aatcacagat gtcaaggtta tcatcccccc tctgcgactc tgcggagaca atgcaggtat
    13561 gattgcctat gccagcgtca gcgagtggaa caaagaaaac ttcgcaggct ggaacctcaa
    13621 tgccaaacca agtcttgcct ttgataccat ggaataaaga gtggctcttt gtcaagtgta
    13681 gtgggtaggc gaaaagctac aatctggaga gtacgaaatt cgtactctct tttttgatgt
    13741 tcaaagtcat caaaatccgt tttttaaaat tgttaaagtt ccggaaacca aaggctttgc
    13801 acttgataac cttaatcaaa ttattggtcg cttcaagttt agcgttggaa taaggcagct
    13861 taagtgcgtt gataatctta tctctatccc ttaaaaaagt ccaaaagatc gtttgaaaaa
    13921 gcggatgaac cgttggcagt tcttgctcaa tcaatccaaa gaaatggtcg gcattcttct
    13981 cttgaaaatg aaagagcagg agttgataga gttcgtagtg aacctgtaat ccctcagagt
    14041 aagaaagcag tttatctcgt acttcccgat gggttaagtg cattcgaaac gtgggctgat
    14101 agaatcgttt ctgacagagc ttgaagctat cctggtgtag taaatcagtc cagtggactg
    14161 atttagcccg agcctagaaa cgaaaaagcg agggttccaa aagcgcttga gcgcccgata
    14221 ctccaaggat tttcggtcaa attggttcat gatttggatt cgtactcggt tcatagctcg
    14281 gctcagatgt tggacaatgt ggaagcggtc aagaacaatc ttcgcctttg gaaagagttg
    14341 cttagcgagc cgataataag ggctgtacat gtccatggtg atgacctcga ccagctcccg
    14401 aacctctctc tgatagcgtt ggaaatggtt tcgaatcacc gcatgagttc gcccgtctaa
    14461 aattgcgagg atggatttgg actcaaaatc ttgggcaatg aagctcattt tgctcttttt
    14521 gaagctatac tcatcccaac tcataacttt tggcaacttg gtccaatcgg tttcaaactt
    14581 aaattccctc agttttcgga tgacggtgga agttgagacc gccaatctgt gggcaatctc
    14641 agtcatagac tgcttttcaa ggaggagctg agcgattttt tgtctcacca tgttggaaat
    14701 ctggcaattt ttcttgacaa tggatgtttg agaaacgacc accttaaggc aattcttgca
    14761 ctgaaagcgg cgctttttga gatgcagtac cgtgggtaaa ccctgacagt cgagaagcgg
    14821 aattttagag gctttctgga agtcgtattt gatcatcttc ccttgacaat gaggacaagg
    14881 aggagcgggg gaatccaact ttgcctgaac gactagatgg gtttggtatt tcagaacaga
    14941 caagattttg atatttttgt cttccaatcc gagcaaatct gtggtattct taagttgttc
    15001 catatgaccc ctttctaatg agttggttag acgcttttca ttataggtca tatggggctt
    15061 tttttctaca agaaacgacc ctataattcc tggggtggga ttacccacta cagaaattat
    15121 agagcccaaa cattccaaag tcttgtctga ctctggaatg ttttctgttc acagttgttt
    15181 tgtaactgcc taataagtaa ccgcgctata acgaggtatc taccgagatt gtcaattctc
    15241 cagtatactg ttataaaaaa caaaactgga ggatttttta tgtcccccca tcgtcccatc
    15301 gactgttccg tagctgccca ataacggagt atagtcggct gaaacaagaa taggacaaaa
    15361 gtgcctcaag aaaagcatcg taatttgaca atattattct gagatgtttt tgatgtaagc
    15421 ccatattttc tcaatgggat tatactcggg tgaataggta ggaagtggta acagtctatg
    15481 ccctgctcct tacacatgtt cattctgtga aaccttgcat tgtccataat gataagggat
    15541 ggtgtatcta aagtaggtag taagaatttt tggaagcaag cttcgaaaaa gtcactcgtc
    15601 atcgtatctt tataggtcat cggagccata gtatattgaa acaaaaaaga gcattagtac
    15661 ttctataaca gtatcgcaat ctctggtgtg cgttgtctcg atcttaattc aaataattat
    15721 atcttcttcc agagagacta actgcctgct caaggctcga tcatattcga gataaaaata
    15781 ggtcttaacc cctgtctcat aaatataaat aggagtcagg tagcttaagt gattcaattc
    15841 tttaaggaac cgatttactt tttctgggtc ttgttcgcag taggtacagc tcttttttag
    15901 actatatccc atagccttta gagcgtaatg aatagttgtt ggaggacagt tgaattcagc
    15961 agctatttca gtcaaataag catcgggatg agtttcaata tagatcttta atttatctct
    16021 atcaactttt tcaattaata gtggaagttt tcttaaagtt agtgtattga aacgttttta
    16081 tacagtgcta aaatttgaag tgtagtgcag atatatttgt aatagtatta tagcaattct
    16141 ttgaagaagg tcgagacaag agaaaatagg tatctttgag atataagcat gatttactat
    16201 aaaattattg agttattcat aaaaatttct ctatcaaata tcataatgcg tcgtttaagg
    16261 agatggttac aatgcaatat agttgtggaa aaataaatat taatatccct gatggatatg
    16321 gagacattaa ggatatagtg ttttcggctc atattatagt taggtataat aatggacatt
    16381 gtggaggtat agatccgcat ataattggac tttgtaaaaa gcagatcaga aggatgtctc
    16441 tatatcctat tttgataatc gtatctagag attcaaaggt tattgatgat tataaaaatt
    16501 tagata
//
LOCUS       DAVKJJ010000042        16060 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000042 DAVKJJ010000000
VERSION     DAVKJJ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16060
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.042"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(107..337)
                     /locus_tag="VW144_001516"
     CDS             complement(107..337)
                     /locus_tag="VW144_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600940.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(355..996)
                     /locus_tag="VW144_001517"
     CDS             complement(355..996)
                     /locus_tag="VW144_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9600941.1"
                     /translation="MKLLKNLGWFLLALLSFFFIYGFIQGLATASLALGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(1008..1742)
                     /locus_tag="VW144_001518"
     CDS             complement(1008..1742)
                     /locus_tag="VW144_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEU9600942.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            complement(1745..1942)
                     /locus_tag="VW144_001519"
     CDS             complement(1745..1942)
                     /locus_tag="VW144_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600943.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            complement(1942..2148)
                     /locus_tag="VW144_001520"
     CDS             complement(1942..2148)
                     /locus_tag="VW144_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9600944.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(2158..2472)
                     /locus_tag="VW144_001521"
     CDS             complement(2158..2472)
                     /locus_tag="VW144_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600945.1"
                     /translation="MWVLGFILFMIFFYSNNSKKIKKLEKKIKRLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            complement(2768..4057)
                     /locus_tag="VW144_001522"
     CDS             complement(2768..4057)
                     /locus_tag="VW144_001522"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU9600946.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFARKIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQTHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVTLPIENALDVYIA
                     VLGEGANIKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFVAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(4135..5382)
                     /locus_tag="VW144_001523"
     CDS             complement(4135..5382)
                     /locus_tag="VW144_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEU9600947.1"
                     /translation="MKLLFRNQAYRLLTLSRFFNAFGASIFNLVFIVYASTLPQASFA
                     VAMANIVMILPTLFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDVISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDRFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNDYFGKQSLPRLMMWETVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFFGLLFTLSIPVGTACFSLVAVWNMQLT
                     WMLFVGFSLLAIFLTILNLKNDI"
     gene            complement(5572..6423)
                     /gene="rgg"
                     /locus_tag="VW144_001524"
     CDS             complement(5572..6423)
                     /gene="rgg"
                     /locus_tag="VW144_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEU9600948.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIMIKSYMKSMDERVELTAEEGKVLHDYLFSTEIWGIYKLNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            6547..6888
                     /locus_tag="VW144_001525"
     CDS             6547..6888
                     /locus_tag="VW144_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9600949.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            6946..7026
                     /locus_tag="VW144_001526"
                     /pseudo
     CDS             6946..7026
                     /locus_tag="VW144_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(7232..8935)
                     /gene="ilvD"
                     /locus_tag="VW144_001527"
     CDS             complement(7232..8935)
                     /gene="ilvD"
                     /locus_tag="VW144_001527"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU9600950.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETPIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(9168..10100)
                     /locus_tag="VW144_001528"
     CDS             complement(9168..10100)
                     /locus_tag="VW144_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEU9600951.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATNNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     SVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSSLCELFSDDGMTKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(10097..10954)
                     /locus_tag="VW144_001529"
     CDS             complement(10097..10954)
                     /locus_tag="VW144_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9600952.1"
                     /translation="MILSKNREDELRKFAANIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPETFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            complement(10958..12304)
                     /locus_tag="VW144_001530"
     CDS             complement(10958..12304)
                     /locus_tag="VW144_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9600953.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFIGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEK"
     gene            complement(12317..12601)
                     /locus_tag="VW144_001531"
     CDS             complement(12317..12601)
                     /locus_tag="VW144_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9600954.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(12605..14635)
                     /locus_tag="VW144_001532"
     CDS             complement(12605..14635)
                     /locus_tag="VW144_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU9600955.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NSHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPIIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKVSSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYNDIKMIIKQED"
     gene            14826..15833
                     /locus_tag="VW144_001533"
     CDS             14826..15833
                     /locus_tag="VW144_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9600956.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            15836..16036
                     /locus_tag="VW144_001534"
     CDS             15836..16036
                     /locus_tag="VW144_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9600957.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
BASE COUNT         5158 a         3414 c         2767 g         4721 t
ORIGIN      
        1 acaacctcaa aacagtgttt tgagcaacct gcggctagct tcctagtttg ctctttgatt
       61 ttcattgagt ataatacaaa aatccgatga acttcaccgg actcttttat tttgaatttt
      121 tgcctgcttt acgcttttca gcgatttcgg ctgcctttcg aggcaagaca atttccgtta
      181 tgtaagccgt cccaaaacgc agtacacctg caataggagc aaagacaact gctagatagt
      241 tatagaagaa atcgcctttg aaggcataag ctagcgctcc aatgatgaaa aatagaacga
      301 ctgcctgaat cactgctaat aaaattactc gtttcatgtg acctcctgac tctattatag
      361 catgagaatc atcaaaaagc cgactaaatt attcaaagcg tgaagagaaa tactgtagac
      421 cagacctttt ctgctaatgt aagccaaacc caaactaaaa ccaaggctaa aatagacaaa
      481 aaattgttgc acatcacctg gaaaatgaat caaggcaaat agaagactag ataccagaag
      541 aaaaatcagg gttcgtttac tattgtcctg cttaggaaag agatagcgtg ctaacatccc
      601 tctaaaaaca atctcttccg tcaaaggagc aaaaataacc acagcaaaga atgagaaaag
      661 tggttgagac aaggtcaagt ctgtcgctat ttgttgattt actgaaggat catctggcaa
      721 gaagaattga acgaccagag ataagaacca aaccaaggca ggaagccaaa taaatcggtt
      781 aaagccgctc ttctcaatat aaacaggagc tttctgatac catttgtaaa tgccgtacac
      841 atatactcca gccaagacca catagagtag agtaacagca tagggtgaag cgcctaaagc
      901 aagcgacgca gtcgcgagcc cctgaataaa gccatagata aagaaaaagg atagaagggc
      961 tagaagaaac cagccaaggt ttttaagtag tttcatagat aactccttta tttgaaataa
     1021 cgttttacca taggtaactg catcacattg atataaacat ggatggctcc tacaagcaag
     1081 aaagctagta actgaatctc tcctgtcaag aaagaaatga taataagaaa aatatataag
     1141 gctggtaaga catattggtg taattggaat aaaattcgaa aactctgttc caaattagcc
     1201 tgacgctccc cttcatcata agaatttata tagttcaaga catcctttgg tgtagcgaaa
     1261 aattccaaat caaactgacg aacaatcgca atggttttaa aaagagattt ttgagcgact
     1321 aagaatacca caaagagtaa gaaagaaagg aaaaatgttt gagggtttgt atgcaatata
     1381 atcacctcac ttaatgaaat aaaaatagcc aatggaatcg ctacacctgt aatattaaaa
     1441 gcaatggttc caaactcaag attccgatac atttgcacat aataggtttc attcagatcg
     1501 tcatccattt cctcttgata caaggaatga aattttctgc ttttctttaa gaaattgaaa
     1561 gtcaaaaaca tactaatgaa acctatcagt aaacaaatag ctgatatcca tggcatcaag
     1621 gcttttacat ctaaaataat ttcgtgggat tcgacacgtg ccttaaacat ccctacaaac
     1681 atgcccaaga acccccccaa gacaatagac atcaaaaata acaatctacg tttctttttc
     1741 atattcattc tcctttttca cttgctagat ttttggattt cttttcaatc cattcaatta
     1801 ctgggatgag agcaaagtag acccaaacaa attggtcgct ttgataggga ttaaaccagc
     1861 ttaggtccat cccaatcagt agaaatacgc tgactaataa agctatgacc actacataat
     1921 aaatcacttt atacttgttc atcactcgtc ctcctccaaa cgaaataccg attcgactgt
     1981 ttcgttgaaa atttgagata ttttcagggc aatgataatg gatggggtgt actcatcccg
     2041 ttctagtagg ctaatggtct gtctggaaac ccctgccagt ttggctaggt cggtttgatt
     2101 gagaccatcg cgagctcgaa gctcttttag acgatttttt agttgcatgt tacacaccta
     2161 ctctccgtca aattcaacgg tttggatatc ctcaatacgt tgcaacttga atttttcttt
     2221 tcccgtatta tctacacgtc gtagctttac ccattcctca tcaacatcca caacttccca
     2281 gttatctggc ccaatataca ctcccgttat aattggttcc tttccaatca tttcttgtaa
     2341 taatctcgac atttctgcgt ttcctttctc ttttcgctca agtcttttga ttttcttctc
     2401 tagtttcttg atttttttag aattattaga ataaaagaaa atcataaata gtataaatcc
     2461 tagtacccac attataactc ctttctgctt cctatttctt aacttgaatt cattgtaaca
     2521 tatctttttc tttttgacaa ttatagttgt caaaaagttt atgatttttg tcattttgca
     2581 aaagaaaaag gtcaggagta gattcctgac cactttatct atcattaata ctcttctaaa
     2641 atctcttcaa accacgtcag cttcaccttg ccgtaggtat ggttactgac ttcgtcagtt
     2701 tcatctacaa cctcaaaaca gtgttttgag caacctgcgg ctagcttcct agtttgctct
     2761 ttgattttta ttgagtataa aatcctagtt tttcaaagat ttctgagaag ttttggctga
     2821 ttgtctcaag tgacacttgc acttcttctc gagtttggtt gttcttgacc gtcacttgtc
     2881 cgctttcgac ttcgctctct cctagggtga tgagggtctt agccacaaag acatcggctg
     2941 acttgaactg agcttttagt ttacggttga ggtaatcacg ctctgctttg aaaccttgtt
     3001 ggcgaagagc ctgtaccaat tccaaggcct tgatatttgc cccttcgccc aagactgcga
     3061 tatagacatc tagggcgttt tcgataggga gggtcacgcc ttgtttttca aggatgagaa
     3121 gcaggcgctc tacaccaagt ccaaaaccaa atccagcagt ttcagggcct ccaaagtaag
     3181 caaccaaacc atcgtagcga ccacccgcac agactgtcaa gtcattgccc tcaatctctg
     3241 tgataaactc gaaaatggtg tggttgtagt agtccagacc acgcaccata ttggtatcga
     3301 tgatgtaatc tactccaaga ttttccaaca tctgacgcac agcatcaaaa tgagtttggc
     3361 tttcttcatc aagaaagtcc aagatagacg gcgcattctc tactgccacc ttgtcttctt
     3421 tttccttaga gtccaagaca cgaagaggat tttcctccaa gcgacgttgg ctatccttag
     3481 acaaggtctc cttgagcggt gtcaaatagt caatcaaggc ttggcggtag gctgcacggc
     3541 tctcaggatt tccaagagtg ttgaggtgca atttgacacc ttgaataccg atttccttca
     3601 aaaaatgggc tgccataacg attgtttcca catcggtagc tggattgcta gagccaaaac
     3661 actcaacacc aatctggtgg aattggcgca agcgccctgc ctgtggacgc tcataacgga
     3721 acataggtcc catgtagtag aacttgcttg gcttttgcac ttctggggcg aagagtttat
     3781 tttccacata ggaacggaca acgggcgcag ttccttctgg acggagggta atatggcggt
     3841 cacccttgtc atagaaatcg tacatttcct tggttacgat atccgttgta tctccgacag
     3901 agcggctgat aacctcgtaa tgctcgaaaa taggcgtacg cacttctgcg tagttgtagc
     3961 gcttgaaaat tttacgggca aagccctcaa cgtactgcca cttagcagac tcagcaggta
     4021 aaatatcctg cgttcctttt ggtttttgta atttcatagg gaatcctctt taaacttaat
     4081 agtcttattt taccataaat agagggatta aaacagtaag aaaaaaatta ggatttagat
     4141 atcatttttg agattaagaa ttgttaaaaa aatagctagc aaggaaaaac caacaaatag
     4201 catccaagtc aactgcatgt tccatacggc tactagtgaa aaacaagctg ttcccacagg
     4261 tatggataag gtaaacaata gaccgaaaaa attactagta cgagctagaa cctctggagc
     4321 tagatttttc atgagcatgg cactaatctt tggttgaact ttaccagaca catacagagt
     4381 aaagaagaga aatagcaaac caagcacgac ttgattgaat aaattagcca aaccaactag
     4441 actaagtcct acggtctccc acatcatcaa tctaggcaag gactgcttcc caaaataatc
     4501 attgcccgta aggctactga tgatgattgc tactaaaaca cagaattgat tgataaatag
     4561 tgcctctgta taagaaaaat tcaagagaga atggctcaaa aagaagatat tataaattcc
     4621 acccaaagcg ccacccaagg aattaataag caagacagca aagagcataa aaccaaagtt
     4681 tttctgtcca cttttaagaa aaacgagacg taaatttcgg taaattgtta ggaaccggtc
     4741 tttgatagaa agcttctcat tttttaagtt ttcaccatca gcagatgaca ttgacaggct
     4801 caatttgctt tttcctaaaa agaggatagt ggctgatact aggaaaaagc aggcattgat
     4861 tcccgcaacg agagaaaaat tattgaccga tagagctaag agccagactc cgaaagcttg
     4921 accaccaata gctgaaatat aggtgatgaa ctgtgaaaaa gaataagcct ccatcaaatc
     4981 atcttcagct actttttcct taataagagg catacgcagg ccacctgcaa aatcactgat
     5041 gacatcacta atgacattga tcaaacacag gctagaaaag gcaaagagac tagcttgctg
     5101 aacaactagg gctgctagaa aaaatagaac cgcctgaaac aaaccgctat agaccatcca
     5161 tttgaccttg tccctcgtgt aatctgcccg aatccctgca aaaactgtaa agagggtcgg
     5221 aagaatcatg acaatattcg ccatagcaac agcaaaagat gcttgtggca aggtcgatgc
     5281 atagacgata aagaccaggt tgaaaatcga agcaccaaaa gcattgaaga agcgtgacaa
     5341 agtcaatagg cgataagctt gatttctaaa caatagtttc atagataatc tccctccttg
     5401 ttctaagttc tacatcaaga atagctttag tatagcactt atgaatttgc gaaacaattc
     5461 ttttgaatat atgcaacaaa taaaatgagc aaaaaacgat agaacttttg taatgacctg
     5521 agaaagggca acagttctat cgcttcaaat tttttcttag tttgcagata ttcaacattc
     5581 ggtcgtattt ctatagtatt cggcagattt attacagcca agcatctcaa aaatacggac
     5641 agcatcctcc atctttttct ggccttcctt gactctacct tgcttgctat caaggagacc
     5701 ttctgcccac agataaacaa ttcggaaata ggtctcattt tccttgtaga aatgctcttc
     5761 gataacacgt ttaaaataat aggcattggt aaattcttca cactcaatac tagctaaaaa
     5821 gccattcaat agtatagtat gaaaaaggtt tcgattgcca gacatttcca ttagaaaatc
     5881 agatttacgt accatttctc gtacatatct agtaaaaaga gaaacagata aaaatggaga
     5941 actgactgaa aataaattga gtttatagat tccccagatc tcggtagaaa acaaataatc
     6001 atgaaggact tttccttcct ctgctgttaa ctctaccctt tcatccatgc tcttcatata
     6061 agacttgatc ataatggcat ttagaatatg tttctgtttg ttgtgagaat gggcatgctt
     6121 ttgatactcc ctgcgatata agtcctcaag aggtgctata ttctttggtt ccaagacatc
     6181 tgtaatttct tttctcaact cagaatctgt atcatactgg aaacctctcg ccagaaagag
     6241 gatctcctcc acactggcag atatattttc cagagcaaat agaaactttt ccaccgaaag
     6301 ctcactctga cctgtttcaa aacgggacaa catagacggc gaaaattgtc ctccggttgc
     6361 ttgtctcagt gagatatttc ttgattctcg taattgtcta aagacttttc caatctgctc
     6421 catagacttc cccttgatcc cgtattttct tcattttatc atatttttca gaaaattcat
     6481 caaaaacttg ccaaattgtc agaatcatga gaaaatagag gatatttatc acgtggaggg
     6541 actgctatga gagacgatat caaaatcaat gaccgtgctt tggccttgca agaccaaatt
     6601 atcgaaaaac tagagaaagt ttttgataca gatgtggaat tggatgttta caatctaggt
     6661 ctgatttatg aaatcaatct ggatgaaacg gggctctgca agattgtcat gaccttcacc
     6721 gatactgcct gtgattgcgc cgaaagcctg cctattgaaa tcgtggcagg tctgaaacaa
     6781 atcgagggta tcaaagatat caaggttgaa gttacctggt cgcctgcttg gaaaatcaca
     6841 cgaatcagtc gctatggccg tattgccctt ggactaccac ctcgttaagc aggccaatca
     6901 cttttaaaga tgaaaaaagc aagccgaaaa tggcttgctt tttgatactc aatgaaaatc
     6961 aaagggcaaa ctagaaaact agccgcaggt tgctcaaaac actgttttga agttatggat
     7021 agaactgacg aagtcagctc aaaacactgt tttgaggttg tggatagaac tgacgaagtc
     7081 agctcaaaac actgttttga ggttgtggat agaactgacg aagtcagccc aaaacactgt
     7141 tttgaggttg tggatagaac tgacgaagtc agtaaccata cctacggcaa ggcgacgttg
     7201 acgtgatttg aagagatttt cgagtatgag tttatttttt acctgacttg tccatattcc
     7261 agaagtctgt cacggctccg cgtgaagcag atgatacgat gtgggcatat ttaccgagga
     7321 caccacggct gtaaagtggt ggcaaggttg tttctgcctt gcgtttttca agttcttctt
     7381 cggatacggc catggaaatt tctttggtat cttggtcaac cgtaacgata tcgccggtac
     7441 ggagataggc aattggtcca ccatcctgag cttcaggagc gatatgtcca acaaccagac
     7501 cataagtacc accagagaaa cgtccgtccg tcaagagggc caccttatct ccctgacctt
     7561 taccaacaat cattgaagaa agtgacagca tctcaggcat accaggacca cctttaggtc
     7621 caacaaaacg aacaacgact acatcgccat caacgatttc atctgtcaga acggcctgaa
     7681 tcgcatcttc ttctgagtca aagaccttag ctggcccaac gtgacgacgc actttaacac
     7741 ctgatacctt ggcaactgca ccgtcaggag caaggttccc gttcaagatg ataagcggac
     7801 catccgcacg ttttggattt tcaagtggca tgataacttt ttggcctgga gtcaagtctg
     7861 caaagtcagc caagttttca gctacagtct taccagtaca tgtgatgcga tctccgtgaa
     7921 ggaaaccatt tgccaacaag tacttcataa ccgcagggac accaccgact tcgtagaggt
     7981 cttggaagac atactgacca gatggtttca agtcggccaa gtgaggcaca cgttcttgaa
     8041 tcgtattgaa gtcctcaagt gacaagtcaa catttgcggc atgggcaatg gcgagcaagt
     8101 gaagagtggc gtttgtagaa ccaccgagag ccatcgttac agtaatagca tcttcaaagg
     8161 cttcacgagt caagatatct gatggtttga gaccaagttc caacatctta acaacagcac
     8221 gtcctgctgc ttcgatatct tctttcttat cagctgattc agctgggtga gaggatgacc
     8281 ctggcaaact catccctaga acttcgatag cagttgccat ggtattagca gtatacatac
     8341 caccacagcc accagggcca gggcaggcat tacattcaag acgtttcacg tcctcagctg
     8401 tcatgtcacc gtggttccat tttccgatac cttcaaagac agaaaccaag tcgatatctt
     8461 taccatcaag atttcccggt gcaatagttc caccataggc gaaaatagct gggatatcca
     8521 tattagcaat agcaatcata gatccaggca tgttcttgtc acagccaccg atagcgacaa
     8581 aggcatccac gttgtgacca ctcatagccg cctcgatgga gtccgcgatg atgtcacgag
     8641 atgttagaga gaaacgcata ccaggcgttc ccatagcgat cccgtctgct acggtaatgg
     8701 ttccaaactg tacaggccaa gcgcctgcag atttgacacc ttctttagcc agtttcccga
     8761 aatcatgcaa gtgaatgtta catggtgtat tttccgccca agtcgaaatc actcccacaa
     8821 tcggtgtttc aaagtcctta tctgtcatac cagtcgcacg aagcatagca cggttaggtg
     8881 atttaaccat gctgtcataa atgctactgc ggtgacgttt atctaattca gtcatcttat
     8941 ccctcccatt tcagttttta ctattatagc acaattttcg catgaagaac agaataaaaa
     9001 tcttgaattt tcagagaatt ctatacacat gtgaaatatt taaaattaaa aacaacaaag
     9061 cggattagtg cactttctga tgaccagaat atgcttttta atccgctttc tttaaataac
     9121 gtactgtaat ttttacagaa attctttcag ataagtgtat ttaacatcta tcttgcatta
     9181 taaatttcta gaaccttctc ttttatattc gattcactca aaccatactc attaagaaga
     9241 taatccattt tccctacttg accgaatctt tcttgaacac ccatccgatg aatttttgtc
     9301 attccatcat cagagaataa ttcacataaa gaactgccaa ttccacctat ctgattgtgg
     9361 ttttctacag taaatatagt ttttccactt aacattgttt ttatctgttc tggtatcggt
     9421 ttgattctaa ataaatctat cacacttact gaataaccta atttagacag ttcatctgca
     9481 actcgaatac ttggagcaac cattatacca gaagcaacga ttacaagatc ttcaccatgc
     9541 cttaactcaa tgtagccttt agaaaaatct tctccacctt gatacacagg aactggagct
     9601 tttctaattg ttcgaatata ttttagtcct tttaagtcta atgtctggtt caatatttca
     9661 cgaaattgga tatcatcagt tgcttcaaaa atgatcgatt taggaattaa acgtaacaat
     9721 ccaatttctt caaatggcat atgtgttcca ccattcatct ctgccgttac tcctgcatct
     9781 gatccaatca cagtggcatc caattgtgcg tatccaagag aaataaataa ttgatcaaat
     9841 actcttcgtg aagcaaaagg accaaatgta tgaagataag gtctaaaccc ctgaatagac
     9901 aagcctgctg caaggccgac catttctgct tccataatcc caacattcac ataacggtct
     9961 ccaaaatcct tttcaagatt attagtagcc atcgaacttg acaaatcggc ttctaagact
    10021 actatatcag aataactttg attagcctct aaaaggaagt ctctatatac atgccgtaat
    10081 tctttcgtgc ttctcatcat tctgtttcct ccaattcctg acttaatctt tctacaactg
    10141 aagttaacat ttgtctctcc tctacagtag ggcgaagatg atggttggat ttcatttctt
    10201 ccagctcttg aaccccttga cctttaatag tatctaatac aatgcactta ggtgatgaat
    10261 tatttgactg ttttaattgg acaatccctt cataaatttc tctaatatct gaacccttga
    10321 ccctaatgga ttcaaatcca aatgctgaaa atttttctac gaaatcacct ggattacaaa
    10381 tatcctttgt aaaaccatct aattgttttt tgttatcatc aacaaataca attaagttgg
    10441 ataactgttg atgagaagca aactgtatag cctcccaaca ttgtccctca tttaactcac
    10501 catctccaac aatagcataa gtataaaagg gactctttct tattctctga ccatatgcaa
    10561 gtccagttgc aacactaatt ccttgtccta aagagcccgt tgtcatatct atgcctggcg
    10621 ttagatttct atcaggatga gacggtaatt tggttccatt tgtatttaaa gaatataaga
    10681 attctttgtc aaagaaacca ttcaaataga gtgtactgta tagagctggt cctccatgac
    10741 cttttgataa tatgaaataa tctctatctc gtgctgcaaa tgtttctgga gtcattggca
    10801 ttatttcacc ataaagcact gctaaaactt ctacgataga cagacttcct ccgtaatgtc
    10861 cgaatccaag atgattcaat gttctaagag tatttaatcg gatgttagcc gcaaattttc
    10921 ttaactcatc ttctctattt ttacttaaaa tcatccctta tttctccttt gcagatggct
    10981 ttttaataaa ggatactcca aacataactg ctaaaataag aacaagacca atcacaatgc
    11041 ctgcttgtga gccaaattga tttaacattc ctaaaataat tcctgataga ccaaaatctg
    11101 catctgagaa agttgatcct tggaaaccga gtcctcccaa aactggcatt aaaaagactg
    11161 ggagaaaact gattaaaata ccttgtaaaa atgctccaat agtggctcca cgaacaccac
    11221 cagatgcatt cccaataaca cctgcagtcg ctccacagaa gaaatgaggc acaacgcctg
    11281 gtaagataac aaccgttcct gaagcaatca taattgccat acttactaaa ccaccaataa
    11341 aactagagat aaatccaatt agaactgcat taggtgcata agtataaaca atcggacaat
    11401 ccaaagcagg ttttgaatta ggtacaagac gctctgaaat acctttaaag gctggaacaa
    11461 tttcgcccaa aataaggcga acacctgcta aaataacaaa tacccctgct gcaaattgac
    11521 ctgctaattg taaagcataa actagaccac ttgtaccact actgatttct ttttctatat
    11581 attctgaccc tgcaaagata gctacaataa tgtaaataac tgccatagat aaagtgatac
    11641 taacagtact atcacgtaaa aaagctaaac tctttggaaa tttaatgtcc tctgttgatt
    11701 ttgatttgtc accgataagg ctaccagtaa aaccactcaa ccaatatccc aaagaactga
    11761 aatgacctaa agccaccttg tcatttccag ttaattgaac catatatttt tgcacaaatg
    11821 ctggggaaat actcataata ataccgagtg ctaatcctcc tagtaagatg agaggcaagc
    11881 tagtaaagcc agcaactgat aaaatgaccg caatcataca tgccatatat agagtgtggt
    11941 gccctgttaa aaaaatatat ttaaatcgag taaaacgagc gattaagata ttgaacacca
    12001 ttcctgcaaa cataatcatt gcagtagctg agccatatgt tgttaaagct acagctacaa
    12061 ttgcttcatt attcggcaca acgccagata aatgaaaagc atgctcaaac atggtaccaa
    12121 atggatttaa agaattttgt acaattcctg caccaccaga tacaactaag aaaccaacaa
    12181 aggtcttaat tccaccttta ataatatcag gtaatttctt cttctgaaga actaatccta
    12241 aaattgcaat taaagctact aaaatagctg gtgtactaac aatatccaat atgaacttca
    12301 tcatgacgct agcctcctat ataagtcctt tttcttcaca aagtttagta attaattctc
    12361 gtagttcatc catatcaata atactattta agatacgaac atctccaaga tgactagctg
    12421 aatcagctag atcacgacca acaatccaaa tatcagctgc atttggatct gctccaccta
    12481 aatcataatg ttcaacttct acatccgaaa cattcaaatc actcaataca gattcaatat
    12541 tcatctgtac cataaaactt gaacctaatc ctgaaccaca agctgtacca atttttaaca
    12601 ttatctaatc ctcctgttta attatcattt taatatcatt atagtttttt gatgatatta
    12661 aagtttgaac atgattttta tctcttaaaa ttgttgttaa atgtgacaaa gcctttaaat
    12721 gactctcatt atcaatggct gcaatacaaa tcaacaatct tacctcttgt tctggattat
    12781 ccaataaata aatcggttct tccaaaacta acattgacat tcctatttca ttcacacctt
    12841 catctggccg agcgtgagga attgctactc ccttccctaa attaataaaa ggtccaaact
    12901 cttctacttt ttgaatcatt gcctcagggt agttctcagt tatcttatct tgatccaaaa
    12961 gcggtttagc tgctaaacga atcgcctcct tccatcctaa tttttgcgaa ctaacctgat
    13021 aggtttcttt ggtaataagt tgttctagca ctggtacaat ttcctttcta tcattttttt
    13081 gataaagata attctttaac gccaatctta attccaattc ttgtgtaata attccatatc
    13141 ttttgacaat attcaggatt tgttcaatct caaaatctcc atactctaaa ttcggaaaat
    13201 cttttaacac tagttctact agttgtattg cttgctcttc agtcatcata accgaaacta
    13261 gataatttgg cttttctgtc tccaccttta tggtagaaaa aaccatatca tagtcactac
    13321 taactttcac ctgtaaatca tcaatctttg aggttcctat aaactcaatt tgaggaaata
    13381 atgctaatag attctctttt aacatcaatg aagaactaac accattagga caaatgattg
    13441 ctgctttata ccatttttga ggcaaagtat ctgctttctt taaataacct ccgaaatgga
    13501 taacaaaata tgctgtttca ctatcaggta tgggattgtc aatagcgtcc atcaagggca
    13561 tcaaagaatc tttgactaat tcaaataaat caggataatg ttctttaaca tgcaatacat
    13621 attcatttga actaggtagg ccgaacttta atctatagta agccggtata aggtggcggc
    13681 gaagattttc tctcaatcct tccctttgtt taaaatgtaa caaagaaata tcttccattc
    13741 tacttataat agcctctgtt aattgattaa agtaaaccgg agcaacatct acttcacctt
    13801 caaagcaact tgataataaa acggtgatat agcgataatc atcctcagaa aacaccgtat
    13861 ctataattcc caaatcaacc actgtatcca ataaaatagt cgttatatct tgaataatag
    13921 gagatactaa tgtctctgaa agacacactc tttcaacatc cctttgatac ctacacagaa
    13981 tgaatactaa accgaaaagg taaactttta attgattaac aataggtact agctgtagct
    14041 tctcataata atctttaact acctgatcaa tcaaatcata agttaatgaa tacccccaac
    14101 tggataaaac ataatccaaa ccccaaatcc ctatggagga ttccagcaac tcactaacca
    14161 tttgaaaagc taagcggtgc ttattccact ctgaaccgtg taaagtataa ccttttgctc
    14221 tactgtaccc tagctccaaa tcattatcta acataatctt tcttaatgat tgaatatcag
    14281 ataaggttgt attcttactt actttcaaaa agtcttggta atgactattc gatataaaat
    14341 ctaatcggca aaaagtgtaa agatagatta aagctaagcg agtcgacttt ggtaaaacca
    14401 attcatccga cttaataata tctgtcaaag actgcttcgt acgatttgat aaactatagc
    14461 gaccttgctt tttatccagc actatccctt tattagctag ataaggcact aaataatcta
    14521 ttccttcttt gacttccttt ataggtaagc tcaccttaac agataattca tataatgata
    14581 gctcacaatg atccatcaaa gtcatcaaaa taactagtgc tctataatca aacatctctc
    14641 ttcctttcta actacaatta tagtgtaaaa attataagaa taaaagagta tttgagcaca
    14701 atataatctc accttttaaa agaaattatt gggctgaaac tttttagtga ttattttcaa
    14761 ctttctattg acttttaaaa acaaagtgat atcatttaga tatcaaataa tgggaggtca
    14821 ttcttatgac tgaaaaacta atcaattcaa aaccaaatgg tgtattcgca ttgattctca
    14881 ttgagttgac aatcgtactt ggtatcttta tatttataat gggggttggt tcggaaaaca
    14941 tttttggaat tattatcgga cctttactaa tcgtaattgc aggtctagct catgctggtt
    15001 taaaagttgt caaacctcag gaagctctgg ttctgacact ctttggtaat tatacaggta
    15061 ccatcaaaga acctggtttt tactttgtca atcccttcag cgtagcagtc aaccctgcaa
    15121 accacactcg acttggacaa agtggtgatg ttagcacaaa atctcctttt ttaggagcta
    15181 aatcatcaaa tgacaatgat gtaaatcttg aaattggcaa gaaacacatt tccctcaaag
    15241 tcatgacctt gagcaattct cgtcaaaaaa tcaatgattg cttaggaaac cctgtagaaa
    15301 tcggtatcgc ggtaacttgg agagttgtcg ataccgctaa ggcagtcttc aacgttgata
    15361 actacaaaga atatctttca ttgcaatgtg atagcgccct ccgtaatatt gtccgcatct
    15421 atccttacga tgtgtctcct aatgtggata ctacgggtga cgggcaagca gatgaaggta
    15481 gtctccgtgg ctctagcgaa attgttgcta accgtattcg tgaagaaatc caaagtcgtg
    15541 ttgaggatgc tggcttggaa atccttgaag cacgtatcac ttacctagct tatgctccag
    15601 aaattgctgc cgttatgctt caacgccaac aagcatctgc cattattgat gcacggaaga
    15661 tgattgtaga tggtgctgta ggaatggttg aaatggcact agaacggctc aatgaagggg
    15721 aattggtaga acttgacgaa gaacgaaaag ctgccatggt ttcaaatctc cttgtcgttc
    15781 tttgtggcaa tcatgatgca caaccaattg tcaacacagg aagtctttac taaagatggc
    15841 tgaagctaaa aaaaagcaga tcccccttcg actctcaaca aagttatacg ctgcactcgc
    15901 atcatgggca gaagacgact ttcgttcagt caatgggcaa atcgaatatc tcctcacaga
    15961 gtgtgtcaag caacggaaga aagacggaaa atacgtatca gaaaccattg acgaaccatt
    16021 tgagattgat atttaacacc ttctcctgtc tgccgtgtcg
//
LOCUS       DAVKJJ010000043        15176 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000043 DAVKJJ010000000
VERSION     DAVKJJ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15176
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.043"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(1..171)
                     /locus_tag="VW144_001535"
                     /pseudo
     CDS             complement(1..171)
                     /locus_tag="VW144_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(217..450)
                     /locus_tag="VW144_001536"
     CDS             complement(217..450)
                     /locus_tag="VW144_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9600958.1"
                     /translation="MKEKEIFDSIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQSVGDVVKIIQGK"
     gene            complement(456..1448)
                     /gene="plsX"
                     /locus_tag="VW144_001537"
     CDS             complement(456..1448)
                     /gene="plsX"
                     /locus_tag="VW144_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU9600959.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETEVVAQTAREFSGE"
     gene            complement(1445..2215)
                     /gene="recO"
                     /locus_tag="VW144_001538"
     CDS             complement(1445..2215)
                     /gene="recO"
                     /locus_tag="VW144_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU9600960.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKCMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDERRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(2212..3381)
                     /locus_tag="VW144_001539"
     CDS             complement(2212..3381)
                     /locus_tag="VW144_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9600961.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAAFTSQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            3530..4540
                     /locus_tag="VW144_001540"
     CDS             3530..4540
                     /locus_tag="VW144_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEU9600962.1"
                     /translation="MSFLSKNGAGILACLLISIVSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIDAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKELSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            complement(4723..5160)
                     /locus_tag="VW144_001541"
     CDS             complement(4723..5160)
                     /locus_tag="VW144_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU9600963.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(5245..7878)
                     /gene="polA"
                     /locus_tag="VW144_001542"
     CDS             complement(5245..7878)
                     /gene="polA"
                     /locus_tag="VW144_001542"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU9600964.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVSESLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYVQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDDVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            8134..8484
                     /locus_tag="VW144_001543"
     CDS             8134..8484
                     /locus_tag="VW144_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU9600965.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQVN
                     QNLEKIRQREGDTH"
     gene            8699..8815
                     /locus_tag="VW144_001544"
     CDS             8699..8815
                     /locus_tag="VW144_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9600966.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            8808..9041
                     /locus_tag="VW144_001545"
     CDS             8808..9041
                     /locus_tag="VW144_001545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600967.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
     gene            9167..9448
                     /locus_tag="VW144_001546"
                     /pseudo
     CDS             9167..9448
                     /locus_tag="VW144_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9582..10550)
                     /locus_tag="VW144_001547"
     CDS             complement(9582..10550)
                     /locus_tag="VW144_001547"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9600968.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(10695..11318)
                     /locus_tag="VW144_001548"
     CDS             complement(10695..11318)
                     /locus_tag="VW144_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEU9600969.1"
                     /translation="MPKKVLILSLLGGMFLSGWLSSFANTYIHDLLGVLFPDSTFLNA
                     FESAIVAPLVEEPLKLLPLVFVLALIPVRKLKSLFLLGIASGLGFQMIEDIGYIRTDL
                     PEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAYKGQKVGKKQGLIFLGLAL
                     GTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(11300..11509)
                     /locus_tag="VW144_001549"
     CDS             complement(11300..11509)
                     /locus_tag="VW144_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600970.1"
                     /translation="MKKSSILFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGISIFL
                     ALFYMIPALYFLFRIGKNGNCQRRF"
     gene            complement(11534..12031)
                     /locus_tag="VW144_001550"
     CDS             complement(11534..12031)
                     /locus_tag="VW144_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU9600971.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(12104..13465)
                     /gene="radA"
                     /locus_tag="VW144_001551"
     CDS             complement(12104..13465)
                     /gene="radA"
                     /locus_tag="VW144_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU9600972.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLQQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(13479..13994)
                     /locus_tag="VW144_001552"
     CDS             complement(13479..13994)
                     /locus_tag="VW144_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9600973.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(13996..14439)
                     /locus_tag="VW144_001553"
     CDS             complement(13996..14439)
                     /locus_tag="VW144_001553"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU9600974.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAAGGVRTGGFGSTGH"
     gene            complement(14499..14592)
                     /gene="ffs"
                     /locus_tag="VW144_001554"
     ncRNA           complement(14499..14592)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VW144_001554"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(14625..15092)
                     /locus_tag="VW144_001555"
     CDS             complement(14625..15092)
                     /locus_tag="VW144_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU9600975.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYNILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
BASE COUNT         4442 a         3772 c         2726 g         4236 t
ORIGIN      
        1 ttctcaacct tgcttctctc cttagatagc gcatgattac aggctttatc ttcaactgtt
       61 agcggcttga gtttgctgga tttacgtgga agtttgtgct tgaggatata tcttcatgag
      121 cccttgataa ccactgtcag ccaagatttt accagcttgt ccgatatttc tcatttctaa
      181 aaaccatcta cttccgttga cttggaactc cgattgctat tttccttgaa tgattttaac
      241 cacatctcct acactttgga gttggtcaat ttcctcatcg ctgatttcga tactaaattc
      301 atcctccagc gtcaagataa actccatcaa atcaactgag tcagcatcca agtcgtcttt
      361 cagactcaag gattctgtca cgacaaagtc ctctccctgt cgctcttgga taatggtcac
      421 aatactgtca aaaatttctt tttctttcat ctcttttatt ctcctgaaaa ttcacgcgca
      481 gtctgggcaa ctacttctgt ttctagcatg gtacgaatct ggcgaatcgt actataaaca
      541 gccttggcat cgcttgagcc atgagtcttg acaacaggtg ccttgacacc aaacaagacc
      601 gctccaccaa catctgaata attgagctgt tttttcaaac ctctgaggct gtccttgaga
      661 aggagggcac ctagtttcgc tcgaagacca ccacctgtaa tagctgtctt gagcaagccc
      721 atgattccca tagctgtccc ttcgatggat ttgagcacag cgtttcccgt gaaaccatct
      781 gccacaacaa catctgcaac accattcatc aaatcacgcg cttccacgtt tccgataaag
      841 ttcaaacttt catcagccgc cagtaattca taagtttcct tacgaagcgg gtcgcccttg
      901 ctactctctg ttccgttgtt gagcaaacca acacgtggtt gcgcaatgcc acgaacattc
      961 ttggcataga aagaacctag gacagcgtat tgatggaggt gctgggctgt attttctgca
     1021 ttagcaccga ggtcaagcat gtcaaaacct ttcccatcta cagtcggcaa tgttgacata
     1081 agtccaggac ggtcgatatt cttgatacga cccacgatga agaatccagc agccaacaaa
     1141 gcacctgtat tcccagccga aaggacagcg tctgcttcac catctttgac agccttggct
     1201 gccaatacca tactggcatt tttcttattc cgaatagctc tcgtaggttc atcatccgaa
     1261 tcaatcttct catccgtatg gataatgctg acgcgctctg tcgctgtcag atattgcttg
     1321 attttagctt catctccgta gagttgaacc tcgatatctg aaaagtcaga tagggcttga
     1381 ttgacaccct caacgatggc ctgaggtgcg taatcgcccc ccatggcatc tactgcgatt
     1441 tttttcattt cttttcctct tttagtaatt gtccccagtc tgctagggaa tcaataaatt
     1501 tctttgattt taggtgaatc ccaacgtact cttcatatag ttgatccata aattggcgta
     1561 gctcttgctt gattccaggc ttgagcgaaa tggtctccaa agtctcaaaa tcaatagctt
     1621 gaaattgatt gagcagatag gggatattgg gattgagatg gcaacgtctc tcatcctcat
     1681 gataatgctc tggacagaga caggctccat atttgaaaga aaagtcaaaa gcctgaccaa
     1741 cccgatggca gaagacacac tcattaaaat tgaggctgat tccaaatcga gtcaaaattt
     1801 gaatttcaaa aatattggtc aaaacctgat aatccaagcc tgcttccatc aactccaaag
     1861 tcttttgcaa aaaagcaaac aagggagcat cctgctgatt gtcctgcaaa ctagcatctg
     1921 caagagctgc cacataggtc gcataggcca tgacaaagag gtcactatta atcttgggaa
     1981 aagtcatgac ctcatgatag tcttcgatgt aactgagtcc gtcatcattg attcgcaaga
     2041 gaaatcgtgc cagcaccaag ggctgaataa caggcgccag cttagactga ccagcgtgtt
     2101 tgacaaaaaa catgcatttg ccaacctgct ctgtaaaaat tttgacaagc ttgtcatcct
     2161 cacgaaaatt gcgattgtaa agcaccaagc cttgactcgt gatagactga atcatgcttc
     2221 tctcatatac tcctcaagtc gtttcatggc ttctttgata gtctccatgc tggctgcata
     2281 agataggcgg acgtagcctt ccccgtaacg tccaaaggct gcaccaggga taaaggcaac
     2341 ggccttcttc tgagcaaaat ccttcagaaa agcaaaggag tcttgattgt agcccgctgg
     2401 aattttagca aaaatataga aggcaccgtc tggtttgata atctcaaaac caagagcagt
     2461 cattttttcg atgatatagt cccgacgctg gatatattcc ttcttcatgg gctccgcatc
     2521 atttttacca gccgtcaagg cttctaccgc agcatgttgc gccatggtat ttgcggcagt
     2581 gaccaagtat tggtgactct tgattaactg ggatgtgaag gccgcaggag cgaaaatcag
     2641 ccccaaacgc caacctgtca tggcatgcga tttagacaaa ccattgataa taatagcctg
     2701 gtctctcaac atcgttccta gagacacatg ggcttcgcct gtgtaggtca attctgagta
     2761 aacctcatca cagacaacaa aaatttcgta cttgcgtaaa acagctgcca aggcctctaa
     2821 ctgctctcga ctgtaggtaa ttcctgtcgg attggctgga tagttgagaa taaccgcctt
     2881 gagcttatca ccctgctcca aaatggcctt ctccaacatc tcaggagtca agacaaaacc
     2941 attttcagtc gtatcaatct caacaatttc tgccccaact aagttaacaa tcggttcata
     3001 gcctggataa gcaggagctg gcaaaagtac cttgtctccc tcttccaaaa tagccgtcaa
     3061 agtcgcagat aaagcctctg tcgccccaat tgtaaccaag atttcatttt caggagcata
     3121 gtccagttgg tacttttcct taacaaagtc actagctgcc tgacgtagag tcagcagacc
     3181 actcatccct gtatagtagg attggttctg atcaatcgct cgcttggccg cctccttgac
     3241 atggtctggc gttgtaaaat caggttcccc caaggtcaaa cgcaagaccc caggaatctc
     3301 cgaaatagcc tggtcaaact gacgaatcaa cgaaacttga attttatcta actgtttatt
     3361 aaagcgctta gttaagtcca tagatacctc ctactttcaa aacgtatctc tattatacta
     3421 caaaagcccc ccgaccgcaa aaatacatta gaggatagct ttccactttc tattttactt
     3481 ttcctgttta taggtctata atggtaatag attttatttg gaggttttta tgtcatttct
     3541 atcaaaaaat ggagcaggta tcttggcctg ccttctcatt tctatcgtat cttggtactt
     3601 aggaggattc ttccctgtga ttggcgcgcc cgtttttgcc attttcatag gcatgctcct
     3661 acatcccttt ctctcgtcct ataaacaact ggatgctggt ttgaccttta gttccaagaa
     3721 gttgctccaa tatgccgttg tcttgcttgg ttttggtctc aatatctcgc aggtcttcgc
     3781 agttggccaa tcttcactcc ctgtcatcct gtccactatc tcaatagctc tgattattgc
     3841 ctacctcttc cagcgtttct ttgccctgga tacaaaactg gctaccttgg ttggagtagg
     3901 ttcttctatc tgtgggggtt ctgccattgc agcgacagcg cccgttattg atgctaagga
     3961 aaaggaagta gcccaagcca tttccgttat ctttttcttc aatgtcttgg ctgcgctcat
     4021 ctttccaacc ctcggcacct ggcttcatct atccaatgaa ggcttcgccc tctttgcagg
     4081 gactgcggtc aacgacactt cctctgtaac ggctgccgcc agcgcttggg acagtcttta
     4141 ccaaagcaat accctcgagt ctgcaaccat tgttaaactc acacgtactt tggccattat
     4201 ccctatcacg ctctttctat cctactggca aagtcgccaa caagaaaaca agcaaagcct
     4261 gcaactgaaa aaagtcttcc cactttttat cctttacttt atccttgcct ctctcctcac
     4321 tacactactc acctctctag gtgtgtccag tagtttcttt actcctctca aagaactctc
     4381 taaattcctt attgtcatgg ccatgagtgc tatcggtctc aaaaccaatc tggtcgctat
     4441 ggtcaaatcc agtggaaaat ccattcttct tggagccatc tgctggattg ccatcatcct
     4501 caccactctt ggtatgcaga cccttatcgg cattttctaa cactcttcga aaatctcttc
     4561 aaaccacgtc aacgtcgcct tgccgtatat atgttactga cttcgtcagt tctatctgca
     4621 acctcaaaac ggtgttttga gcagcccgcg gctagtttcc tagtttgctc tttgattttc
     4681 attgagtata acacaaaagg tagcccatca gctacctttt tcttatgctt cctcaatcaa
     4741 gcgagtatgt tctctcttga tacagcgatt catcacgata tcatcacatc caccatcacg
     4801 caaaatctct ttcgcttcta aactttcaag tcctagctgt gcccaaaaaa tcttggcatc
     4861 agctttgaga aaatcacgcg ccacatcggg cagaaattca ctgcgacgat aaacattgac
     4921 aatatctaca ggaaaaggaa tttcagcgag gctagcataa gccttttcac ccaagatttc
     4981 gccacctgcc gccttgggat tgactgggat gattttatag ccccgagcct gcatttcctt
     5041 tgttactcga ttgctggttg tttcttcacg gtcagacaaa cccaccacag caagggtttt
     5101 actcgttgcg agatactgac gaatcacgcc atcacttgga ttgataaatt cttgactcat
     5161 agaaatcctc ctttttcatc agtatagcac attttgaaaa ggtttgcaga attatactac
     5221 aaaaaaggag gactagcccc ctttttattt agcctcgtac caggttgccc cttcattctc
     5281 atctgcgata agaggaacac tgagttgaat ggcttcttcc atggtttgtt tcaccaattt
     5341 tttcatctct accaattcag atttaggcac ttcaaggacg atttcatcgt gcacttgtaa
     5401 cagcatctta gtctgataac cacctgcaac caaggcttta tccagctgaa tcatggcaat
     5461 cttgagaata tctgctgccg aaccctggat aggtgagttg atagcagttc gttccgcaaa
     5521 accacgaata ttgaagttgc gcgaattgat atctggcaac tcacggcgac gcttaaagag
     5581 ggtctctaca tagcccttat cacgcgcctc ccgcaccact tcatccatgt agtttttaat
     5641 acctggaaaa cgttcaaagt aggtatcaat gtaggctttg gcttccttac gactaattcc
     5701 caaattatta gacaagccaa agtctgaaat cccataaacc actccaaagt taactgcctt
     5761 ggcattgcga cggtcgtttg cagtcacatc atcaggacgc tcaatgccaa agacccgcat
     5821 ggctgtcgaa gtatggatat ctgccccctc ttggaaggcc ttaatcaagt gctcatcctt
     5881 agaaatatgc gccaaaacgc gcaattcaat ctgtgaatag tcagagctga gtagcacact
     5941 atcctcccac tctggcacaa aagccttccg aatcaagcgc ccctgttcca atcgggcagg
     6001 aatattttgc aagtttggat ccacactaga caaacgcccg gtctgggtca aatcctgcac
     6061 atagcgagta tgaatctttc catcagccaa aatccagtcc tgcaagccaa ttacataagt
     6121 agattgaatc ttagcaattt gacggtaatc caggattttc ttaacaatcg gagcaatagg
     6181 agcgagacgc tccaaaacat ccactgctgt cgaataacct gtcttggttt tcttagtgta
     6241 ttctagagga agtcccaatt tctcaaagag aagcacgccc aactgcttag gcgagttgac
     6301 attaaactcc tcaccagcca gctcgtaaat ctcttgagtc agtttttcaa tgacaagctc
     6361 attttcagcc tgcatctcaa gcaaggtctc tttcttgacc ataatcccag caatttccat
     6421 cttggcaagg acaaaagcca gaggttgctc catatcataa agaagctcta attgcccatt
     6481 ttcgctgagt ttttcaagta aaataggctc tgtttctacc aaaacagcaa gtttacaagc
     6541 taagtgttcc aagaatttct cacgttcagg aatggccttt ttaacaccct taccgtagaa
     6601 agtttcatca tcaaccaagt aagtctgacc ataaagacta gcgatggtcg caatttcatt
     6661 gtcctccaca gtcgaaagga ggtatttagc caaacggatg tcaaaagcag gcgcctgcaa
     6721 atccacacca aaacgttgca aaagaacttt aaccttctta aagtcataaa ctctcagaga
     6781 tgttttttct aagaaatcct tgaaaatcgg gtcttgcaac agctcaagct tgtctgtggc
     6841 atagagctta tccccacaag accaggcaaa tccaaccaaa ttatccgtat ggtaattctc
     6901 accaaaaagc tcaaagtgga agatagactc ttcactcagc atatcttgac tgatttggtc
     6961 aacaatagta aaatccaaac tctcagacac atcagctgac gacacattta aagcctgctt
     7021 tagctgtttg aagcccatct catcgtagaa tttcccaaga ttttcaacat ctggaccact
     7081 atagaccaag tcctctaaac caatcgcaat cggtgccttg gtatcaatgg tcgctagtgt
     7141 tttagacaaa aaggcctgtt ccttgtcatt gatgagattt tccttcatct tagaagtctt
     7201 cattccatca atattttcat aaatcccctc aagcgaacca tgctccagca agagcttaat
     7261 acccgtcttt tcaccgactt tggtcacccc agggatatta tccgacttat cacccatgag
     7321 cgccttgaga tcgataaact gagctggtgt gaggcccatt tcttccatga ggtaatctgg
     7381 cgtaaaggcc tcaaactcag ccacaccttt cttggaaatt tcaaccaccg tatgctcatc
     7441 cgtcagctga atcaaatcct tgtccccact gacaatggta atatcaaaac catcctgctc
     7501 tgctagctta tccagcgtcc caatgatgtc atccgcctca tactgagcca gctcatagtg
     7561 acgaatcccc atatgatcca gcaactcacg aatgaaagga aattgctcac gaaactcatc
     7621 aggagtcttg gcccgaccac ccttatagtc cgcatacatc tctgtccgga aggtcgtctt
     7681 tcccgcatca aaagccacca aaatatgact cggctcaacc cgctccaaca aatgactcaa
     7741 catcaactga aaaccataaa tcgcattggt atgcaaacca gccgcattct taaaacggtc
     7801 caactgctga tacagcgcaa aaaacgcccg aaaagctaca gaagacccat caatcaataa
     7861 taattttttc ttatccatac acccattata aaggaaagaa tcaaaaaata ccattgggaa
     7921 gagctagagc aagtattttt caaacttttt ccgaataaat agatagagcc agagaattta
     7981 gtaaacctag atttaaaaat gtgctataat atagtatatt gaatctataa tagtacacct
     8041 tgactgctaa aatatttcta taaattaatt tgactttcct gatagagtta ttcacatctt
     8101 atttcaactc actatagaag gaggaatagg aggattctca gacatccggg catcagccca
     8161 actaatgatt tgattgctaa gaaaatattc agcaatccag aaatcacttg tcaatttatt
     8221 cgcgatatgc tggacttgcc agcaaaaaat gtgaccattt tggagggaag cgatattcac
     8281 gtattactct ccatgcctta ctcggtgcag gatttttata ccagtataga cgtcttggcg
     8341 gagttggata acggtactca agtaattatt gagattcaag tccatcatca gaattttttc
     8401 atcaatcact tgtgggctta cctgtgcagt caggttaatc aaaatcttga aaaaattcgt
     8461 cagcgagaag gtgatactca ctagatctac aaacacatcg ctcctgttta cgtcattgct
     8521 atcgtggata gtaattattt ctcagatgac ctggcttttc atagctttag tatgcgcgaa
     8581 gacacaacag gtgaggtatt ggcgattacc aacaatggac aggaaaacca tctggttaag
     8641 atggcattct tggaattaaa aaatacagag aaaccagcaa agacaaggtt cgcaagccat
     8701 ggttggagtt tttcggcaac aagcccttta cccagcaacc gcaacgagcc attacccaag
     8761 caaatcaact gctggactac aagagctggt ccgaggagga caggaaaatg tttagtcaac
     8821 tacatatgcg agaagaacaa gtcttgttag cacaggacta tgccttggaa actgctaggg
     8881 ctgaaggcct tgaacaagga ctagagcgtg ggaaagttga aggaagggca gaaaggaaac
     8941 tttttgcctt cctagacata gtacgccaag gtcttctgac ttctgaggtt gccagccagc
     9001 aattaggtat gtcagtatct gaatttgagg cactgttgta aaatggctcc ataatatcca
     9061 taggggattt acccactatg gatattatgg agcctatttt gtgtaaaaaa aagtcccata
     9121 tgacctataa tgaaaagcga caaaacaact cattagaaag aatcatatgg aacaattaca
     9181 ttttatcaca aaattactag acattaaaga ccctaatgtc cagattttaa acatcatcaa
     9241 taaggataca cacaaggaaa tcatcgccaa actggactac gacgccccat cttgccctga
     9301 gtgcggaaac caattgaaga aatatgactt tcaaaaacct tctaaaattc cttatcttga
     9361 aacgactggt atacctacta gaattctcct tagaaagcgt cgattcaagt gctatcactg
     9421 ttcaaaaatg atggtcgctg aaacttctga tgacgtacag tcatatttct tctcttttta
     9481 ttatatcaca gttttaaatc tagctttact agattcaccg ctactatcta tttattcgga
     9541 aaaaagacga aaaaacctga gaatcatctc aggcttggtc attaaatttt tttctcaata
     9601 tcgaaaagtg gagaaagtgg tcgtttttca tgaatacgta cgatagcatc ccctaggaga
     9661 tgagcgattg aaatctgctc aatcttatca atcaaacgct cttctggcag atagatggta
     9721 tccaaaacaa ccaatttctt aatagctgat ttttggatat tgtccgtagc aggaccagaa
     9781 agaactgggt gcgtacagct tgcatagact tcaacagcac cagcttccgc aagagcatct
     9841 gccgcatgac aaatcgttcc agcggtatca atcatatcat caatcaagat acaagtcttg
     9901 ccttcaacct taccgatgat gttcataact tcactagtat tcatcttatc aacgctacga
     9961 cgtttatcaa taatagcgat agatgttttc aaaaattctg ccaacttacg agcacgagtc
    10021 acccctccat ggtccgggct gacaaccaca tagtcagaac caaccatacc acgacgctca
    10081 aaataatctg caatcagagg agcacccatc aaatgatcca caggaatatc aaagaatcct
    10141 tgaatttgcg cagcatgcaa gtcgatggtc aataaacgat ccactccagc tacttcaagc
    10201 atatttgcga caagttttga agtgattggc tcacgcgctc tcgcctttct atcctgacgt
    10261 gcatacccat agtaaggcat gacaacatta acagattctg cactcgcacg cttcaaagca
    10321 tctaccataa tcaaaatttc aagcagattg tcatttacag gcgaactagt tgattgtaag
    10381 ataaagacgt gtttcccacg gattgattct tcaatgttga cctgaatctc tccatctgaa
    10441 aattggcgaa cacttgattt ccccaactct atcccaatct cctgcgccac acgttctgcc
    10501 aattctttat tagaagaaag ggcaaacagc tttaaatcag aaaaagacat gatttcctcc
    10561 ggtatatatg tataacttgt gcttttcaca agattttcca tctaccattg tagcgctttt
    10621 tgcactattt ttcaatcaaa aataaaagaa gggcaccata tttgtaccct tgcatcattc
    10681 ttttgaaaaa tattctaggt catcaactca ttgtgtttct caacaaagca ataagcatga
    10741 taaaaaccat agagagcaat agccgtaacc actggaatcg ctaaaggcaa ctctgtttcc
    10801 aactccacaa aaggagagtt aaacaagaag tgagttccca aggctaaacc tagaaaaata
    10861 agtccctgtt tcttgccaac cttctgtcct ttataggctc tgtaaagcaa gtaaacacct
    10921 actacagcta gacctgaaaa agtccagtga gaggcaattc ctgagatgat acgctctaaa
    10981 attcgcgaaa tagtaaagtc aaagccctct ggcaaatccg tacgaatata accaatatcc
    11041 tcaatcattt ggaatcccaa accggaagca attccaagta aaaacaaaga ttttaatttt
    11101 cgcacaggaa tcaaagccaa aacaaaaaca agtggcaata atttcaaggg ttcttctacc
    11161 aaaggagcca caatagcact ttcaaaggca tttaaaaatg tactatctgg gaaaagaacc
    11221 cccagtaaat catggatata agtattagca aaactagaca accatcctga aaggaacatc
    11281 cctcccaata aagacagaat caaaaccttc tttggcaatt cccatttttc ccaatacgga
    11341 agagaaaata aagagccgga atcatgtaaa agagagctag aaagatagaa attcccatta
    11401 gtccatattc cgcacctgac ctcgaaccgt ccgtatagta gatggtttca tactgtaaac
    11461 caatacatag caataaaata aaaataaata aaatactgct tttcttcata cactttcttt
    11521 ctaaatgaag tatttataat tctacgactg tcatacttcc tgtatcaaca ttgtaaatag
    11581 caccagagat aatgacatcg tctggtatta ggggagactc gataagcagt tgcatatcct
    11641 cgcgtacact ctcttctata tcttggaagg gcaagaagtc ctggtctgac acatctacac
    11701 ctaattcctc ttttaaatac tcctgaaaag gttcattttc aaaggtctga gcaccacagt
    11761 ctgtatggtg caataccaca atctctcttg tccccatttg ttgctgggaa ataactagcg
    11821 aacgaatcat gtcttcagtc actcgaccac ctgcattccg caagatatga gcatccccaa
    11881 gtgccaagcc cagagcttgc gcaacgtgca gacgagagtc catacaggtc acaatagcta
    11941 ctcttgtttt gggtttaagt ggcagattta actgcccatg tagggcaaca taagcctgat
    12001 tggcttgcat aaactgttca aaatacgaca cgattccctc cttgaaaatt tgatagtcaa
    12061 atatttctcc tatcttatca tttttaagag aatttgtcac ggattatgca aagacctttt
    12121 tcaaaacttc ctgaatcgtt gtcacgccaa tgacctgaat ttccttaggc agagtgattc
    12181 ctgtcaagga attcttaggt acataaatct tagtaaagcc cagtttagca gcttcgttga
    12241 tgcgttgctc aatacgattc acgcgccgaa tctctcctgt caagcccagt tctccgacaa
    12301 aacattcctg aggattagtt ggtttgtctt tgtagctcga agcaatagca actgcaacag
    12361 ccaagtcaat ggcaggttca tccaatttaa caccaccagc agatttgaga taggcatcct
    12421 gattttgcaa gagaagccct gcccgttttt ccaaaacagc cataatcaag ctagcacggt
    12481 taaaatcaag tcctgtcgta gtacgcttgg catttccaaa catggtcggt gttactaaag
    12541 cctgaacctc cgccaaaatc ggacgcgtcc cttccatggt tacaacgatg gaggaaccag
    12601 tcgccccatc caaacgctct tctaggaaaa cttgactcgg attgagtacc tcaaccaagc
    12661 cgcccgactg catctcaaaa atcccaatct cattagtgga accaaaacga tttttgaccg
    12721 ctctcaaaat acgaaaggtg tggtgacgct ccccttcaaa gtaaagcacc gtatccacca
    12781 tatgctccaa catacgaggc ccagccaagg ttccttcttt ggtcacatga cctacgataa
    12841 agatggcaat gttattggtc ttggccaact gcatgagttc agcggtcact tcacgtacct
    12901 gagaaacaga cccctgcacc cctgaaatct caggagacat gatggtctgg atggaatcaa
    12961 taataagaaa gtctggctgg atacgctcca cttctgcacg aacactctgc atattggtct
    13021 ctgcatagag ataaaactca ctatcaatat cacctaagcg ctctgcacgt agtttaatct
    13081 gctgggcaga ctcctcccca ctgacataga gaactgtccc cacttgggac aactgggttg
    13141 agacttgttg gagaagagtt gatttcccaa tcccaggatc cccaccgatg aggacgagac
    13201 ttcctggtac cactccgcct ccaagcacac ggttgaattc ctccatctcc gtcttggttc
    13261 gattgacatt gatggaagtc acctcagcta gtttcatggg cttggttttc tcacctgtca
    13321 aggacacacg cgcattcttg acctcggcaa cctcaacctc ttccacaaaa gaagaccaag
    13381 atccacagtt ggggcaacgt cccagatatt taggggaatt atacccacaa ttttgacata
    13441 caaatgtcgc ttttttcttt gcgatgacaa acctcttcct atatctctaa ctcacactca
    13501 atcacttggc aaaaatcaat cttctcattt ggcacaaact ggcgcatgag cattcgatga
    13561 gcaacaacta ccacagtctg atgttctcga tacttagaca tacattctag aaaccgagac
    13621 ttcatttccg tagctgtctc atattgaata ggactattag gaagcaactc ccccttgttt
    13681 tctaaaaaca gtcttctagc tgtttcaaag ttttctattc ctgttttata gacctgccat
    13741 tcatgtaata aaggctctac tcttaaagga agacccgtag cacagaccac atacgaagcc
    13801 gtttctaaag ctcttgtgac tgcagaagat acgattattt cagctgacga gagtaaagga
    13861 tttttgctca atttctggac ttgctgccgt cccatctcag acaagggtgc caaatctatc
    13921 ccaaatccta tataagaacg ctcctctaac tcacggtaat ctggctcccc atgacgtaca
    13981 aagataatct tcattctagt gccctgtcga tccaaatcca ccagttcgaa cgccaccagc
    14041 tgcatctcca tctgcaatta agaaagtagc aaaaacagcc tggacaatac gttccccaac
    14101 ttcaagaaca acctcttggt ctgtgatatt cttcatctgc gcaaaaatat gcccttcatt
    14161 tccaggattt ccataataat ccccatcaat gaccccaact gagttaatta aaaccaagcc
    14221 cttcttacga ggatttgaag aacgatcata gaggtagaga acctcagtcg gctgcatata
    14281 agccttaacc cctgtcggaa ccaagacaat ctctcctggc gcaacaactg tacgcacagc
    14341 aacctttaag tcgtaaccag ccgcatgcgc tgtctcacgc ttgggcaata aattttcatc
    14401 tgtaaaactt gaaaccaatt caaaaccacg aattttcata attttctctt ttctattatc
    14461 atttattcta gattattcta tcttatttat tcggaaaaag cacgaaaaaa gagcacacaa
    14521 ttcaaatcgc ttagggctgc tggattcctc ccctgacccg cttcacgcag aactgttgct
    14581 ccactattta ttatatcaca ttcctattca ttttaaaagc aaaattattt tttccgtcta
    14641 tttctaaaaa aatcctgcat gatagctgcg cattcatctt ccaaaattcc tatttcaacc
    14701 tccacacgat gattgagacg ctcatctgtc aagatattgt acaaactccc agcagcgcca
    14761 aatttctggt ttttagcccc atagaccaca tttggaatgc gggcaagccc aatcgctcca
    14821 ctacacatga cacaaggttc aatggtcaca aaaagtgtgc aatccagcaa gcgccagctc
    14881 tcctcactca agttcgcatc ctctatagcc ataatttccg catgcataac cgctcgctgt
    14941 aattcctcac gcgcattatg cccacgacca atgatttccc catctttgac aatcacacaa
    15001 ccaattggaa tttcatcgtg ttcaagagca atctcagcct ctctcaaagc ctccctcata
    15061 aagacttctt tttcttcaag cgtataatac atctcttttc tcttttccta cttatcgatt
    15121 ttattattat atcatgaatc ccaacacaaa aaaagccacc gaatgcggtg actcta
//
LOCUS       DAVKJJ010000044        14086 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000044 DAVKJJ010000000
VERSION     DAVKJJ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14086
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.044"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(<1..778)
                     /locus_tag="VW144_001556"
     CDS             complement(<1..778)
                     /locus_tag="VW144_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9600976.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     EDLFYEQLPNEEKVWFEATRATIDVIRSGRPEYGETVLDDYFKTIYDKELFLINELEV
                     INLYFAIVLTKIKQGQNQIEEINRIHSFLVRLTNHVELIASEYLFALSNTLFSGLACL
                     DNLSSYDSLGAYIFSLNHIMEKTQDFQKKPIILMLEWKLSL"
     gene            complement(850..1410)
                     /gene="rnmV"
                     /locus_tag="VW144_001557"
     CDS             complement(850..1410)
                     /gene="rnmV"
                     /locus_tag="VW144_001557"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEU9600977.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTVIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(1410..2183)
                     /locus_tag="VW144_001558"
     CDS             complement(1410..2183)
                     /locus_tag="VW144_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU9600978.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     RALELVDEYDQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELLLDKILVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(2488..3153)
                     /locus_tag="VW144_001559"
     CDS             complement(2488..3153)
                     /locus_tag="VW144_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEU9600979.1"
                     /translation="MTILGKDTVQQSAKGESVTQEATPEYKLENTPGGDKGGNTGSSD
                     ANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKNAIEKAAKDKQDEIKGAPL
                     SDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGVQAIAMVTVPKRPVAPNAA
                     PKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALASLGLVVATSGFALLGRKTR
                     RRK"
     gene            complement(3416..3550)
                     /gene="rpmH"
                     /locus_tag="VW144_001560"
     CDS             complement(3416..3550)
                     /gene="rpmH"
                     /locus_tag="VW144_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU9600980.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(3695..4909)
                     /locus_tag="VW144_001561"
     CDS             complement(3695..4909)
                     /locus_tag="VW144_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9600981.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKKLLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(5065..5358)
                     /locus_tag="VW144_001562"
     CDS             complement(5065..5358)
                     /locus_tag="VW144_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600982.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVEVIMICQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            5520..5972
                     /locus_tag="VW144_001563"
     CDS             5520..5972
                     /locus_tag="VW144_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU9600983.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(6007..7395)
                     /locus_tag="VW144_001564"
     CDS             complement(6007..7395)
                     /locus_tag="VW144_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9600984.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAADKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            7461..8423
                     /locus_tag="VW144_001565"
     CDS             7461..8423
                     /locus_tag="VW144_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU9600985.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVNTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(8452..8655)
                     /locus_tag="VW144_001566"
     CDS             complement(8452..8655)
                     /locus_tag="VW144_001566"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600986.1"
                     /translation="MNTIERTRRLVKGCATHCFEVADRTDEVSSKHCFEVVDRTDEVS
                     SKHVFEVVDRTDEVSNHTYGKAT"
     gene            complement(8719..9729)
                     /gene="ccpA"
                     /locus_tag="VW144_001567"
     CDS             complement(8719..9729)
                     /gene="ccpA"
                     /locus_tag="VW144_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEU9600987.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGIAYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(10154..10753)
                     /locus_tag="VW144_001568"
     CDS             complement(10154..10753)
                     /locus_tag="VW144_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9600988.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDITILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(10755..11852)
                     /locus_tag="VW144_001569"
     CDS             complement(10755..11852)
                     /locus_tag="VW144_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9600989.1"
                     /translation="MLERLKSIHYMFCASLIFMLFPILPVVIGELPAWHLLVDILFVV
                     TYLGVLITKNQRLSWLFWGIMLVYVAGNTVFVAGNYIWFLFFLSNLLIYHFGVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYMLVILTFVDLMTLGSVRIRLVEDLKEAQ
                     VKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTVR
                     ERVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(11852..12589)
                     /locus_tag="VW144_001570"
     CDS             complement(11852..12589)
                     /locus_tag="VW144_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9600990.1"
                     /translation="MKRWIALNKIEFLLTKRQLVYYLLSVGMPTAFYLFFSGIYQETP
                     GGPANFMRDYLISMTAFSMMSTAMFSFPAVLHTDKINNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFSVGHFVRGVEIPLGSWVGAAILLILGSVAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAIGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(12591..13475)
                     /locus_tag="VW144_001571"
     CDS             complement(12591..13475)
                     /locus_tag="VW144_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9600991.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFVLTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLDTIFEETQKGDD"
     gene            complement(13462..13656)
                     /locus_tag="VW144_001572"
     CDS             complement(13462..13656)
                     /locus_tag="VW144_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600992.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFIRGALGFGFM
                     IFVVFLMGMEWLKGESHDGG"
     gene            complement(13653..13859)
                     /locus_tag="VW144_001573"
     CDS             complement(13653..13859)
                     /locus_tag="VW144_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9600993.1"
                     /translation="MGEEEMRNKMIIAVSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT         4148 a         3263 c         2522 g         4153 t
ORIGIN      
        1 ttagagataa tttccactcc aacattaata taattggttt cttctggaaa tcttgtgttt
       61 tttccattat atgattaagg ctaaagatat aagctcctag tgaatcataa gacgacaagt
      121 tatccaaaca ggctagaccc gaaaacaaag tgttacttaa agcaaacaga tattctgatg
      181 caattaattc tacatgattt gtcaaacgaa ctaaaaacga atgaattctg ttaatttctt
      241 caatctgatt ttgaccttgt tttatctttg taagcactat agcaaaatat aagttgataa
      301 cttctaattc atttatcaaa aacaattcct tatcataaat tgttttaaaa taatcgtcca
      361 gtacggtttc cccgtattct ggtcgtccac ttcgaataac atctatcgtt gctctagtag
      421 cttcaaacca tactttttcc tcattaggaa gttgctcata aaataaatcc tctatctctt
      481 ccaaacacgc ttccttctta tcgtactcct cttctttacc atagattggt tcacgtaaaa
      541 tctggtattt caattctaaa taagcagaag gaagagctga aaaatccggc ataaggctat
      601 aaactggaac tcctagccgg cgagcaatat aggctaactt gggcaaactc ggttgcgaag
      661 ctccactttc aattcgcgac agttgacgaa ctgtcagttc ttgctcatct ccacaaaaat
      721 ctggacgact aattcctttt tctatccgaa tctctttaat tttttgacca attaacatca
      781 gacgcctcct attctacctg cttcgaaaaa tactgtgaaa cttaaaaaat tgtaacggct
      841 acatctttcc tactcataag atttcatagc ttcttccact tctgccaaag taaccccaaa
      901 caactctagg cgtttgagga gttgcttgcc gttggaatag ccgattcgga gagtctctcc
      961 gaggtattct ctacgcttac ggctgtctgc ccctgctaga aaaccaaggc gaatcaaatc
     1021 gctacgacta atgtcaaact ggctctcatg ttcaaattgt tctgtcacct gagctagagc
     1081 cgttttcagg tcttcatagc tggcatgctc aattcccaga gaacgcccct tggtcttgga
     1141 cttgggaaca gcttcatctc gcttgagaaa ggcatgctga actgttggaa tgaccgtcat
     1201 gatcatgcgc cgaatccgtt ctccattaaa atctgggtct gtaaagacaa tgactccatg
     1261 acgttggtgc aggcgctgaa tccgctctat atcctgatca ttgatggcag aacctcgagt
     1321 ctcataggtc tccacatcga aataacgctt gagattgacc gtatcatcac gcccttcaac
     1381 cacgataact tgagaaattt tctctttcat tacttgctgt ccagtccaaa aattcgttct
     1441 gcatttgcag tcgttgctac cgccagctct tctgtcgtca taccacgcaa gtcagcgata
     1501 aagtcgacca catagcgagt ataggctgtt ttattttcac gaccacgctt gggtacaggt
     1561 gctaagtaag gcgcatctgt ttccaccaaa atcttgtcca aaagtaactc tttagctgct
     1621 tcttggaggt cagttgcctt cttaaaagtc accactcctg agaaggaaat ggtcatacca
     1681 agatccacaa acttctctgc ccactcaagc gtccctgaaa atgaatgcat gataccacca
     1741 cgaggaccaa cgccctcact cttgataatc tcataggtat cttccagcgc atcacgggta
     1801 tggacaacaa aaggcaaatc caagtcctta gatagctgaa tctgacggcg aaaaacctgc
     1861 tcctgcacct ctttgggcgc tgtcatccaa tggtaatcta agccaatttc acctaaagcc
     1921 acaaccttgg gatgttttaa cttatccaac aagtaagcct caacttcctc tgtataagta
     1981 ccagcttctg taggatgcca accaatagtc gcatagagct gatcatactc atctaccaac
     2041 tccaaggcac gctcaatcgt cggtttatca aaaccaacaa tattcatctg tgtcacaccc
     2101 atctcagcag ccaaggcaat ttcttctgcc tcacgacctg caaattcttc tacattcaag
     2161 tgtgtatgtg tatcaaaaat catctcttct aacctcgttt tctatcttct attataccaa
     2221 aaaagaggag gggcacctaa tttttcggtt tcccctcctc tcttcaatag agagctattc
     2281 tgctatcttt tctatccgat attgcccatc tcctattcca cagttagaga cagaagagat
     2341 tggctacatt gatagaatat tcctattcag ctaaagtatg tgattatctt gggttctcac
     2401 ttttctcacc catcactagg attgatgact tctcaaaatc ccccttataa tctaacttta
     2461 agtagagaat agaattttct agctgttcta ttttctacgt ctagtctttc ttcctagcaa
     2521 agcaaaacca cttgttgcca ccactagacc aagactagca agtgctgcac ttgatgctga
     2581 acctgtgtta ggtaattgtt tgccagcagg tgactggtag gtaacatctt gcattgttcc
     2641 tgcagttgct tgcggtgcac ttgttgtctt aggagcagca ttaggagcca ctggtctctt
     2701 aggaactgta accatggcaa tggcttgcac tccaatcgtt tctgcttcct tcacatcttc
     2761 catagttttc gcattttcaa tctctttgag agctgcttgt ttttctgctt ccactcttgc
     2821 taaaagttct gctttttctt tatcagaaag cggtgcgcct ttgatttcat cctgcttgtc
     2881 cttggctgct ttttcaatgg catttttagc tgattctttt tcagtagcta attgcttaga
     2941 agcttgtgat tgagctgagt tttgtgaacc tgtgtgagct gatccacccg cctggctacc
     3001 accgccttca ttcgcattag catctgagct tccagtattg cctcccttat ctccacctgg
     3061 tgtattttct agcttatact ctggtgtagc ttcttgagtt acagattcac ctttcgcaga
     3121 ttgttgaact gtatcttttc ctagaatcgt cactactgaa ccatctggga atgtgattgt
     3181 tgctgtacca tctccagcta cttcaacttt atagtaaaat aaaataataa caggacaaat
     3241 cgatcaggac agtcaaatcg atttctaaca atgttttaga aacagaggtg tactattcta
     3301 gtttcaatct actataacat cgcttccgaa tccatttgca tctactgaac ttttttacaa
     3361 aaataaaaat cagtagactc gagttcctac tgatttttga tagaattcgt ttggattatg
     3421 cagccaaaac tttgcgtcct ttacgacgac gagctgccaa tacgcgacga ccatttttag
     3481 ttgacatacg gttacggaat ccgtgtttgc gcgcacgacg aagtttactt ggttgataag
     3541 tacgtttcac gatgaatacc tcctcataga ttttgtattc gtttagccgg ctataaagtg
     3601 atcagttact aaacatactt tactattcta tctgattctt tcacttttgt caatagccct
     3661 aggcaaatgt ttccagcttt ttcgaatgca agccctatct acgatactgt ttcaagaaac
     3721 gagtcatctt ttcttggatt tgggctaact catcaacacg aggaaggtaa acgatacgga
     3781 agtggtctgg ttcctgccag ttaaagcctc gaccatgaac caagagaacc ttttcctgct
     3841 tcaagaaatc aaggacaaac tgctcatcat catcgatacg gtacatattg cggtcgattt
     3901 ttgggaagat atagagcccc gccttgggtt taacggcaga caaacctgga atatcttgaa
     3961 tggcattata gatgaaattt ctttgctcgt agattcgtcc accaggaaga agcaattcat
     4021 cgactgattg gtgacccccc aaggaagttt gtacgacttg ttgggccaaa acgttagagc
     4081 aaaggcgcat attggacagc atattgagcc cttcgatata gcccttaaca tgagtcttag
     4141 ggccagacaa gaccatccaa cccacacgga aacctgctat gcggtgggat tttgacagac
     4201 cattcatgct gacacagaag acatctggtg ccaagctcgc cacaggcgta tgcacatgtc
     4261 cgtccattac catgcggtca tagatttcat ccgcaaagat aatcaaatcg ttttgacggg
     4321 caatctcaat aatctccagc aagagtttct taggataaag ggctccagtt gggttatttg
     4381 gattgataag gacgattgcc ttggtattgg aagtgatttt tgacttgata tcgtcaatat
     4441 ctgggtacca ttcaacagct tcatcacaga tatagtgaac ggcatttcct ccagctaggc
     4501 tgacagccgc cgtccataga ggatagtctg gcattggcac taagacctca tccccattgt
     4561 ccaaaagccc ctgcatggac atgacaatca actcactgac accatttcca aggtaaatat
     4621 catcaatatc tacgtttggg aatttcttca gctggcaata ctgcatgatg gccttacggg
     4681 ctgagaaaat tcctttggag tcagaatagc cttcactatc acgcgcattc ataatcaagt
     4741 catgaatgac ctcgtctggc gctgtaaagc caaattctgc tggatttcct gtattcagac
     4801 gtaaaatctt ttctccattc gctcgcatcc gcatggcttc ttccaataca ggaccacgga
     4861 tatcataagc aacatgctct aacttactag acttgttata ttctttcatc ttgtttcctc
     4921 aattcatcag gatagtaaca ttataccact agaaagagaa tgcgacaagt ttactgaaat
     4981 gtgttactag tttcgtatag aagaaaaacc ttgctaaagg cgaggttaag aacgtagaga
     5041 tagcaggtat tttgcaaaga cacatcacat accaaaagat aaacctagaa aaagcaagcc
     5101 tagaaaggag tcataaagaa gattaaacat gaggaaaagc ccccatatca tcaagtaatc
     5161 ccaacgccca cttcgttttg caactagaaa taagattaga tggtagagta catgaaaaac
     5221 taaaaatcca ataaaacttg gataaagaag cggtaccaga ctctctaatt gacagatcat
     5281 gatcacttct accaagactg aaactaggat aattccataa taaaggaaat ttgatttttg
     5341 aaaagagttt ttcatcattc tcccttcctt cacttctttc ctgctatcct ttcttttatt
     5401 ttatcatata tttatataga gtttaagccc taatattctt ttgaaaaata ctttttttgc
     5461 tttacttcta tagtgaaaag gtttacaatt atagtaagaa aatttcagga ggtttcatta
     5521 tggcacaacg ttaccaaaat atcatggtcg caatcgatgg ttctaaggaa gcggacttgg
     5581 cttttgtcaa gggagttcat tctgctctac gaaacgacgc taaactcacc atcgcacatg
     5641 tcattgacac acgcgctctc caaagcgtat ccacctttga tgctgaagtt tacgaagaac
     5701 tccaagtcga cgctgaaagt ctgatgaaag agtacgaaaa acgtgctaaa gatgctgggg
     5761 tagcagatgt tcatatcgtc attgaaatgg gaaatccaaa gaccctgcta gcacgtacta
     5821 ttccagatgc cgaggaagtg gacctcatcc tcgttggcgc aactggtctc aacgcctttg
     5881 aacgcctctt ggtcggctct tcatctgaat acatactccg ccatgctaag gtcgatttgc
     5941 tggttgtgag agaacaagaa aaaaccttat aatcacaaag aaaaggagcc cctagctcct
     6001 ttttgtttac gatttatttc tctctttatg gcgttcgtaa gccttgagct ggcgctgcag
     6061 ttccttttta atagcaggtt ctggagcata tttttcttcc caattatctg gttttaagat
     6121 tttatgggtc actggatcaa aatgagcctt gccatctgga aaaattttcc ccatattggc
     6181 ctgatggaca atatcaaaaa tacgttctgg gtccaccccc atcaagacaa aactgccgta
     6241 ggtgaagtaa agcgtgtcaa tcaaggcatc cacttgccct atcaaatctt gctgagcagg
     6301 tgtcttcttg gctactttat ctgctgcctt atcaagggcc tgatgaagtt gcgatacagc
     6361 ttgaccaaaa tcttcttcag aaggactggc tgctcgaaca aactccacca attcttctat
     6421 tttaaaacca gccctatggg ttgcaccctc taaatcccaa gctcgaggtt cttcttgggt
     6481 tcgttcatcc atcatgtggt ggaaggtctt gaccttattg aaatgatagt cacggctgac
     6541 aaagactttt tctgaagaca aaacaccaaa atgttccaag gccttgtgga tcccatcttg
     6601 ttgattgcta gtggtaatgt gcttggcagc ttccttgaca ctgctactac catttcccat
     6661 agcgaccgac ataccgacgc cagccagcat ttcaaggtca ttatctgagt caccaaaagc
     6721 catgacttga ttgaggtcaa agccatattc tttcccaact cggcgaatgc cttctaattt
     6781 agaatttccc tgattgatga catccgatgc aaaaggattg ctacgtgtca atttcaagtc
     6841 ttcaaaatca gctgccgcct tctcagattc ttctggtgtc atcagcatca aaacttggta
     6901 gataggctga ttcatcaggt gaagcaggtc ctcttccttt tggggaacaa ccttgctgac
     6961 catgcgatta aaagactgac tcaccgtccg agttaaaaca gagggaacga agcgactaat
     7021 tcgttgggaa aaagaaccca gaccaaagga catgatttta gaacccaaca tggcatcctt
     7081 ggtccctagg gcaatctccg tgccctcttt tttagcatag ctaattagat ggcgcaaatg
     7141 taacttggaa atagggctcg tgaacaagac tctgtcttta ctaaagatat actggccatt
     7201 ataggttacc gcaaaatcca gatccaaatc gtccatcaat tccttaacaa aaaaaggtcc
     7261 tcgccctgtc gctacgccaa ctagtacccc ttgttctttg acaatcttaa tcgcatcctt
     7321 agtggatttc aaaacactct tgcgattgtt gaccaaggtt ccatcgatat caaaaaaaac
     7381 agctttgact tccatcctat cccaatctcc ccttttgtga tacaatgatt ataccacatt
     7441 tcagaaagag ttagtaaatc atgcctaaga aaatccttgt tttacatacg ggtggaacta
     7501 tttccatgca ggccgatgct tctggcgctg ttgtgacgag ttcagataat cccatgaacc
     7561 atgtgtccaa cccacttgaa ggaatccaag tccacgcctt ggactttttt aaccttccaa
     7621 gtccccatat caaacccaaa catatgctgg tcctctacca gaaaattaaa gaggaagcag
     7681 ataactacga tggagtggtg atcacacacg gaaccgatac tttagaggaa acagcctatt
     7741 tccttgatac catggaagtt ccccatatgc ctatcgttct aacaggagcc atgcgtagct
     7801 ccaatgagct cggtagtgat ggtgtttata attacctaag tgctttacga gtggccagtg
     7861 atgacagggc tgctgacaaa ggagttttgg tcgttatgaa cgatgaaatc cacgctgcca
     7921 agtatgtcac caaaacacat acgactaatg tcaacacctt ccagactcca acacatggcc
     7981 ccctcggtct catcatgaaa caggaaatcc tctacttcaa aacagctgaa cctcgtgttc
     8041 gctttgacct tgatcacata caaggtttag tccctatcat ctcggcttat gctggtatga
     8101 cagatgagct gattgatatg ctggatttag aacacttgga cggtttgatt atccaagcct
     8161 tcggagctgg taatattccc aaagaaacgg ctcaaaaatt agaaagcctt ctgcaaaaag
     8221 gaatcccagt cgctctggta tcacgatgct ttaacggtat tgccgagcct gtttatgcct
     8281 accagggtgg gggcgtacag ttgcaaaaag caggcgtttt ctttgttaaa gaactcaacg
     8341 cccaaaaagc ccgcttgaaa ctcctcatcg ccctcaatgc cggactaaca ggacaggctt
     8401 tgaaagacta tatggaaggc taatactctt cgaaaatctc tgcaaaccac gtcacgtcgc
     8461 cttaccgtat gtatggttac tgacttcgtc agttctatct acaacctcaa aaacatgttt
     8521 tgagctgact tcgtcagttc tatctacaac ctcaaagcag tgctttgagc tgacttcgtc
     8581 agttctatct gcaacctcaa aacagtgtgt tgcacagcct ttaaccagcc gcctagtccg
     8641 ctctatggta ttcattaagt caacatctct tgtttaagag cacaaaatca ggaaatcttc
     8701 tcgattccct gattttttct atttacgttt tcgtgttgag ctacgttctg tcaaaccatg
     8761 aggtaagaga acttcacgtt cttccaactc ttccttatgc ataatcttgg tcaacatacg
     8821 catactaatg gcaccaaggt cataaagagg ttgggcaatc gttgtcaagt ttggacgggt
     8881 aaagcgtgag atttgtgaat catcactagt aataatttca aaatcttctg gcacagaaac
     8941 acccttatca gccaaaccgt tcaagactcc tgctgccaac tcatcacctg tcacaactgc
     9001 tgcagttgca tttgatgaaa tcaaacgctc tgctaaggcg taaccatcat catagctata
     9061 tttagattca aataccaaac cctcactata agcgattcct gcttttttca aggtttcctt
     9121 gtagccaact aaacgaacct taccattgat gtcatccact agcggaccgc taacgaaagc
     9181 aatacgctca ttttctttag caaggtaact cactgcatca attgttgctt gcttatagtc
     9241 aatattgaca cttggcaact ggtgctcaac atcgacagtt cctgcgagaa caatcggagt
     9301 acgtgaacgc gaaaattctg agcgaatttt atctgtcaag tgataaccca tatagataat
     9361 gccatctacc tgctttgaaa agagggtatt gacaacagaa acttctttct cgttatcttc
     9421 atcgctatta gctaggacaa tattgtactt gtacatttct gcaatatcat caatcccctt
     9481 agccaaactc gaaaaataac cattggtaat atttggaatc acgacaccga cagtggttgt
     9541 ctttttactt gcaagaccac gcgcaactgc atttggacga taatccaaac gatcaattac
     9601 ctctagcact tttttacggg tattctcttt tacattttta ttgccattga ccacacggct
     9661 gaccgtcgcc atggaaacac ctgcttcacg agcgacatca taaatggtta ctgtatcatc
     9721 tgcattcatt ccttttcctg tcctttctat ctcacacatt cttttacaag tagaggtact
     9781 gattgaagct ctatatctac ttacaaaagt gaagatgtga aaatttcgtt ttcatatttc
     9841 tacttattcc attctatcac taattgtaaa cactttcaag tgttttttga agattgattg
     9901 aaaaaatttc atagaaaacc taggtttagc tccttgctac caccttagac taaacaaaaa
     9961 ggaggaaact aagccctcct aaagttatag taaaatgaaa taagaacagg ataaatcgat
    10021 caggacagtc aaatcgattt ctaacaatgt tttagaagta gaggtgtact attctagttt
    10081 ccatctacta taggtattgt tccattcact accgtcaatt ttagcacata gtcttcatga
    10141 aaatattata tcatcataac caaccagatt cttttgcgat attagctgcc tctgttcgat
    10201 taccagcatc cagttttgaa agaatattgg tgacatagtt tcgaatagtt ccgtttgaga
    10261 ggtaaagctg atctgcgatt tcttggttgg ataagccccg ggcgattccc tttaagacag
    10321 caatctcttg ttctgtcaac ggattgggac gggtcatcac cacttccatc aattcaggcg
    10381 aatactcctt gcgtccttcg aggacggtgt gcaaggtttg catgaggtct gcaatgcttc
    10441 gttcctttaa tacataagca tccactccag ccttgaccgc acgttcaaaa tacccagcac
    10501 gcttgaaggt cgtcaccaca accacctttg tttcaagcct ttctgatcgt atccactcca
    10561 agacttcaag acctgtctta acaggcattt ctacgtcaag gatggtgata tctacagact
    10621 ccttttctaa tagttggatt gcttcttgcc catttttggc ttgaaggaca gactccacat
    10681 ctggttgaag cgtgagcaat tgacacatgg catctcgcaa catactttga tcttctgcga
    10741 ctaatacttt catcttagtt tctctcctta taaggtagtc gaacttgcac ttccgttgga
    10801 tgtttctgac tgattacact tacttctcct gaaaatggaa aaacacgttc tcggactgta
    10861 tggagctcat ctccttttag aaaagcaaag ccgcagccat catcacttac tgttaaaatg
    10921 agttccttct ctgtccgttc taattttaag taagctttag acgctttggc atgtttgatg
    10981 atattggtca ccaactcaag caaaatcata gaagccattg actccaattc ttgagtaagg
    11041 ctagcagtat ctagttggtt atctgtttcc acctcaattc cagcaatttc taacatcttt
    11101 ttcacagtct ctagttcgga tgtcaaagtt ctagacttaa gattttccac aatggttcga
    11161 acttcacgca ttgattcttt gctaatttgc tgtatttctc ttaattcctt ttccacttgt
    11221 ggataagcct gcatttgaaa tagctgcaag gctagatctg tcttgacact aagcatagca
    11281 aaggtatgtc ccagactatc atgcaaatcc tgaccgatac gattgcgctc attttctgct
    11341 agcaatagat ttatctgggt attttgcttg acctgagctt ctttcaaatc ctcgacaagg
    11401 cgaattcgaa ccgagcccaa agtcatcaaa tcgacaaaag taagaattac aagcatatag
    11461 actagaaact caacttcgat tctctgaaaa atcaaaagtc gccccacaac aaggacttga
    11521 gcaaggagaa atgtccatac atgtaaagac tttaaactac gtacgccaaa atgataaatt
    11581 aagagattgg aaaggaaaaa taaaaaccag atataattcc cagcaacaaa gacggtattc
    11641 ccagctacat agaccagcat gatcccccag aaaagccaag ataggcgctg gttcttagtt
    11701 attaaaacac ccaaatacgt cactacaaat agaatatcaa ccaataaatg ccaagcagga
    11761 agctccccaa tcactacagg taggatggga aaaagcataa aaatcaaact ggcacaaaac
    11821 atatagtgta tgcttttcag tctttcaagc attaagcatt ctccttatga ccttgaaggt
    11881 aaatagtcaa accaaataaa actgctgaaa aaacaagtaa ataaactgtg gctgatagat
    11941 tgatgccacc ctcgtttaag aaggtcttga gcaactccat caactgataa gttgggagac
    12001 acttaccgat cgcttgcatc cagtctggaa ataaagagat aggcatccag agtccgccta
    12061 aaacagccaa tcctagataa agaagattgc ccacaacaga catcagctga ctagttggta
    12121 agagagtcaa ggtcaagcca agggctacaa aagctacact tcccaaaata agcaaaatcg
    12181 cagccccaac ccagcttcct agaggaatct ctacacctct tacaaagtgc ccaactgaga
    12241 aaaccaccaa gattgaaacc aaataatcaa ccagcatact tgttatcttt gatagataat
    12301 attctaccat atttacaggg ctatgacgta atgttttctg ccagttgttg attttatctg
    12361 tatgtaaaac agctgggaat gaaaacatag ctgtcgacat catggaaaag gctgtcatgg
    12421 agatgaggta atcacgcata aaattagctg gtccacctgg tgtctcctgg tagatgccag
    12481 aaaagaataa atagaaagcc gtcggcattc ctacggataa taaataatag acgagttgcc
    12541 gtttggtcaa taaaaattct atcttattta atgctatcca tcgtttcatc ttagtcatct
    12601 cctttttgcg tttcttcaaa gattgtatcc agcaaactac gattattaac ttcgatttct
    12661 tgcatcctac atcctgcttg ggctaatagt tcccagaaag catctgcttc tcgagtgact
    12721 acttgtaaag aatcttgctt tagggtccag ttttcaacca aatttgactg ctctacgact
    12781 tccttgtaag ctataggaag gataaagtgc ttttctattt cctcgctacg catagctaga
    12841 ggcgtcgtat ctcgaatcaa ctctccctta tttaagagca aaatgcggtc tgcagtgtgt
    12901 tccacttcct caatataatg ggacgaatag agaatggtga ctccttgcgc ttttaactcc
    12961 tggacaattt cccaaaaacg ttgacgagtt gaggtatcca tggacgcagt aggctcatct
    13021 aaaaaaacaa tctttggtcg cccaattaag gtcaagacga aagaaaagag acgcttttga
    13081 ccgcctgata atttttctgc caactgttct ttttgttgct tgacaaactg caatagttga
    13141 tcaatttcct gattgctcaa aggatttgga tagatacgtt gaaagaaagc aatcaactct
    13201 ttaactttta atttctgaac gattacattt tcttgaggga ggtaagccct agtatagtct
    13261 aacttagaac tcgtcactgg taaaccttga atagatactt gaccgcttgt gaccagttta
    13321 tctccaagca aacaatctaa gagtgttgtc tttccggcac catttggacc aattaaggca
    13381 acacattcac catcgttgat ttcaaaggag atattccgca aaatttcctt gtcttttatt
    13441 ttcttactca atttctcaac tttaaccacc gtcatgagac tctcctttca accactccat
    13501 tcccataagg aaaacgacga aaatcataaa tccaaacccc aaagcaccac gaatgaattg
    13561 gcgaagcaag gcttgatcaa accaatcagt aaacatttcc actaaccata ccaagagtga
    13621 caggccgata aagaaataga tgatccctct cttcattcct caaactcctt tttcacatct
    13681 ctgactaatt tcaaaccttc tctaacaagc caagacatga ttccaaagcc agcaaagacc
    13741 ttccaaggaa aatggtagaa atcctcatcc aatcccgaaa acatgagata ggtcataact
    13801 cctgctacta ctaaacttac tgcgataatc attttatttc tcatctcttc ttcccccatt
    13861 tcatactaca attatagtct tttaaaatca gaggagacag aagcttctgt cactagaaaa
    13921 tatgacaaat gtcataaaaa attctgttca aaacaagcaa gatacactat acaataaaac
    13981 acaatcagaa aaatctaagg caacttcctc aaaagagata tcaaacccaa ttcacaccat
    14041 aatgtaaact aatacttatt taaaatcaaa aagagtaaaa attttt
//
LOCUS       DAVKJJ010000045        13416 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000045 DAVKJJ010000000
VERSION     DAVKJJ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13416
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.045"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(317..2575)
                     /gene="glgP"
                     /locus_tag="VW144_001574"
     CDS             complement(317..2575)
                     /gene="glgP"
                     /locus_tag="VW144_001574"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEU9600994.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIDLDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGENSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(2601..4118)
                     /gene="malQ"
                     /locus_tag="VW144_001575"
     CDS             complement(2601..4118)
                     /gene="malQ"
                     /locus_tag="VW144_001575"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEU9600995.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            4668..5939
                     /locus_tag="VW144_001576"
     CDS             4668..5939
                     /locus_tag="VW144_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9600996.1"
                     /translation="MSSKFMKSTAVLGTVTLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            6050..7342
                     /locus_tag="VW144_001577"
     CDS             6050..7342
                     /locus_tag="VW144_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9600997.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            7344..8186
                     /locus_tag="VW144_001578"
     CDS             7344..8186
                     /locus_tag="VW144_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9600998.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            8439..9239
                     /locus_tag="VW144_001579"
     CDS             8439..9239
                     /locus_tag="VW144_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEU9600999.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTDKVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            9249..10235
                     /locus_tag="VW144_001580"
     CDS             9249..10235
                     /locus_tag="VW144_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9601000.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            10371..10451
                     /locus_tag="VW144_001581"
                     /pseudo
     CDS             10371..10451
                     /locus_tag="VW144_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(10571..11512)
                     /locus_tag="VW144_001582"
     CDS             complement(10571..11512)
                     /locus_tag="VW144_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9601001.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(11490..13253)
                     /gene="aspS"
                     /locus_tag="VW144_001583"
     CDS             complement(11490..13253)
                     /gene="aspS"
                     /locus_tag="VW144_001583"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU9601002.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEALAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSAEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
BASE COUNT         3903 a         2954 c         2579 g         3980 t
ORIGIN      
        1 gtaagcgaat ataatagatg tgaggtaacc aactacgaaa aaatataagg ctgaagacag
       61 taaaaaccag catttaaagc cttcactgta tctaggattt ccctattttt agacaagatt
      121 gacccaaatc cacagcattt aaaaaagcga atttcaattg aaactcgctt ttttacaaga
      181 tgtattagta taaatttgta tcttatactc aatgaaaatc aaagagcaaa ctaggaagct
      241 agccgcaggt tgctcaaagc accgctttga ggttgcagat aaagctgacg tggtttgaag
      301 agattttcga agagtattag ttcaagtgcc agatgtcttc gttatactga gcgattgtac
      361 ggtcagatga gaagaatcct gctttagaaa tgttaacgat gactttatcc aaccatgcgt
      421 cacggtcttc gtagtcagca agcatttgct ctttgacttt gatgtagtct tccaaatcaa
      481 gaagagtcat gaaccagtct ttgttgatca attcattgta aagacgttcc aagcgctctt
      541 tgtttccagc tgcaagaact gcatcactaa cgatgaagtc aaccaatggt ttgatagctt
      601 cacgagcgta gaattcgctt gatttgtaag ctgcttttgc gtaaaggtcg ataacagttt
      661 ctgaattttc accgaagatg tagatgtttt cttctccaac caactcagcg atttccacgt
      721 tagcaccgtc catagtacca agtgtcaaag ctccgttcaa catgaatttc atgttaccag
      781 tacctgaagc ttctttagaa gcaagtgaga tttgttctga gatatcacat gctgggataa
      841 ggaaacttgc tgcagtaacg ttgtagtttt caaccataac tacttgcaag tgtggagcta
      901 ctgctggatc gttagcaata acttctgaca tgcaaaggat taaatggata atgtcttgag
      961 cgattgtgta ggctggagct gctttaccac caaagaagat tgtgattgga cgagcaggga
     1021 tgttaccagc tttgatgtca aggtatttgt ggatcacgta caaagcgttc atttgttggc
     1081 gtttgtactc gtgaagacgt ttgatttgga tatcaaagat agaatttgga ttgatttcca
     1141 caccttggtg ttctttcaag tgacgagcca atttacgttt gttgtgagcc ttgatgcttt
     1201 ccaatttttc tttgacagct gctttgtctt cataagacaa cagtttttca agctcatctg
     1261 cttcatggtg ccaaccatct ccaagaatct catccaagta gtgagacaat cttgggttag
     1321 catgcataag ccaacgacgg aaagtgatac cgtttgtttt gttgttgaac ttttctgggt
     1381 aaaggtcgta gaaggctttc aactcagaat ttttcaagat ttcagtatgg agtgctgcaa
     1441 ccccgttaac actgtatccg tagtggatat ccatgtgagc catgtgaaca cgtccgctct
     1501 catcgatgat ttgaacagct ggatctttgt actctgcctt cacacgacgg tccaattctt
     1561 cgatgattgg taccaagtga ggaaccactt cttgcaagaa ttcaagaggc catttttcaa
     1621 gcgcttcagc aaggattgtg tggttagtgt aggcagtcat gctacgaaca attgagattg
     1681 cttcgtcaag atcgatacca cgtgcagtca aaagacgaat caattcagga atcaccattg
     1741 atgggtgagt atcgttgatt tggacaactg cgtagtcagc aaggtcatgc aagttgcttc
     1801 ctttttcgat tgcttcgtcg atgatcaatt gcgcaccgtt tgaaaccatg aagtattgtt
     1861 ggaagatacg gagcaattca ccttgacggt cactatcatc tgggtaaagg aagagagtta
     1921 agttgcgagc gatatctgtc ttgtcaaagt tgataccatc tttaataata gaagaatcaa
     1981 ctgaatccaa gtcaaacaaa cgcaagcggt ttttagtcgc tgtttcataa ccagtaacat
     2041 caatatcgta aagagttgat gtcaaagtaa agtctgcaaa tggtacttgg tagctacggc
     2101 ttgagcgaac caaccagttt tgctctgtca accatgcatt tggaattgtt tcttgttggt
     2161 tgtttttaag aacttgttgg aaaagaccaa agtggtagtt aagaccaaca ccgtcaccat
     2221 tcaaaccaag agtagcaatt gagtcgataa agcaggcagc caaacgtccc aaaccaccat
     2281 taccaagaga tggttccaat tcaacttctt caacttcgat caagtcttta cctgcagctg
     2341 caagttcttt tttaacatcg tcgtaaagac caaggttaat caagttgttt gacaagagtt
     2401 taccaatcaa gaactcagct gagatgtagt aaactttttt cttaccagtg ttgactggtt
     2461 tttggctgct tgcaagcttg ctgtagttaa gaagagcaag gtaaagctct tcattgctac
     2521 attctgcaat ggttttattg taacgatttt gtacaaattc ttgtagtgat aacatgttta
     2581 gttgtctcct gattattgtc ttatttcttt aaatctacca aattttcatt aattcggcga
     2641 taaattgttg tcaagtcaag caaaccttcc tcgacagctg gtgtcaattg atcttcagtc
     2701 atacgccaag accagtttcc accaagggta gatgggaagt tcatacgagc tgcctcatcc
     2761 aattctagta aatcttgcat agttgcaatt gccataaagc taactgatga aaatactgta
     2821 cgaagcatag cgtgtaccac tgtttcgtat tctttacggt tcgtgtaacg agccatgtac
     2881 tcacgagtcg catcatcaat ctcattacgg taccaaccaa gaaccgtatt gttatcgtgt
     2941 gttcctgtgt acataactga gttagcaggt gccaagtgtg ggctatcaat gctttcgtct
     3001 tctgggttga aggcaaattg aagaatcttc attcctggga agccagtacg ttcacgcaat
     3061 tcgatcactt catctgtcat gaagccaagg tcttctgcga tgatgtttag ctcaccaagt
     3121 tcttccttaa cggctgcaaa aagcttgtag cctggacctt tcacccactc accaggtgct
     3181 gctgtatcgg aaccagcagg gatttcccag taagattcga agccacggaa gtggtcgata
     3241 cgaacgatat cgtagatttt gaagctttca cgcaagcgtt caatccacca tttgtagccg
     3301 tctttgtcca ttgcttccca gtcatagatt gggttacccc aaagctgacc agttacagaa
     3361 aactcatctg gtgggcatcc tgcgatacaa gtagccttac cattgacatc tgttttgaag
     3421 agatgtggat ttgcccacat atcacttgaa tcttccgcta cgtagattgg catgtcccca
     3481 acgatttcga tgtggttgtc gttagcgtaa gctttcaatt tcaaccattg ttggaagaag
     3541 aagtattgag tcacacggtg gtaaaccaac ttgtctgcca attgctcacg atagctttca
     3601 agtgctgaag ctttacgagc acgagcatct gcatctggcc attcagtcca agcaagattg
     3661 tcaaaatact ctttgatagc catatactca gcaaagagct caagccatga ttggttgtct
     3721 tgagcaaatt tctcaaaatc tttaacatct ccgacttcaa agaaacgttt caccgctttt
     3781 tctaaaagag gacgacgtgc atagtagatt ttagcatagt caacttcaga cgcatcgcta
     3841 ccaaagtcaa ctccttcaag gtcacttgct tccaacaaac cttgctccac caagatatct
     3901 aaatcgataa aatgagtgtt tcctgcgaag gctgagaaag attggtaagg agaatccccg
     3961 taactagttg ctcctaatgg aaggatttgc cagtaacgtt gttttgtacg gaccaagaaa
     4021 tcaacgaagt cgtaagcact ttgaccaaat gatccgattc cgtaagctcc tggaagagaa
     4081 gagatgtgca tcaacacacc actttgacgt tttttcataa tagcacctcg tgtgttaaaa
     4141 taatggaacg ttgcgtattt tgcagacgca aacgtttgcg ttacttataa gtatactcct
     4201 tttcaagcat aattgcaagc gtttttaaaa aacttttttg attattttta aggagactaa
     4261 ctcattttag caaagataac ttaaaactat ctaatcctaa ggtttgactc tcacgagaca
     4321 gtctacaaag tacaaaacct ccttagggag cttgaattta tatgtaacaa agcacaaacg
     4381 tccatagaaa atagataggg attagaacca gggaggtagc ccctcctggt ttccctcttt
     4441 agacagattc catatatttt tattaaaaat actgtattct tatctatttc atacttttct
     4501 tctattaaaa tgcaaaatta tgctatcaaa tcaagcttaa aaatttttta aaatttttac
     4561 aaaaaatact tgcaaccgtt ttctatttgt gctatactaa gctcataaag gaaaacgttt
     4621 gcgtttcctt atcaataaaa cttgctattc tttgggagga atacactatg tcatctaaat
     4681 ttatgaagag cactgcggtg cttggaactg ttacacttgc tagcttgctt ttggtagctt
     4741 gcggaagcaa aactgctgat aagcctgctg attctggttc atctgaagtc aaagaactca
     4801 ctgtatatgt agacgaggga tataagagct atattgaaga ggttgctaaa gcttatgaaa
     4861 aagaagctgg agtaaaagtc actcttaaaa ctggtgatgc tctaggaggt cttgataaac
     4921 tttctcttga caaccaatct ggtaatgtcc ctgatgttat gatggctcca tacgaccgtg
     4981 taggtagcct tggttctgac ggacaacttt cagaagtgaa attgagcgat ggtgctaaaa
     5041 cagacgacac aactaaatct cttgtaacag ctgctaatgg taaagtttac ggtgctcctg
     5101 ccgttatcga gtcacttgtt atgtactaca acaaagactt ggtgaaagat gctccaaaaa
     5161 catttgctga cttggaaaac cttgctaaag atagcaaata cgcattcgct ggtgaagatg
     5221 gtaaaactac tgccttccta gctgactgga caaacttcta ctatacatat ggacttcttg
     5281 ccggtaacgg tgcttacgtc tttggccaaa acggtaaaga cgctaaagac atcggtcttg
     5341 caaacgacgg ttctatcgca ggtatcaact acgctaaatc ttggtacgaa aaatggccta
     5401 aaggtatgca agatacagaa ggtgctggaa acttaatcca aactcaattc caagaaggta
     5461 aaacagctgc tatcatcgac ggaccttgga aagctcaagc ctttaaagat gctaaagtaa
     5521 actacggagt tgcaactatc ccaactcttc caaatggaaa agaatatgct gcattcggtg
     5581 gtggtaaagc ttgggtcatt cctcaagccg ttaagaacct tgaagcttct caaaaatttg
     5641 tagacttcct tgttgcaact gaacaacaaa aagtattata tgataagact aacgaaatcc
     5701 cagctaatac tgaggctcgt tcatacgctg aaggtaaaaa cgatgagttg acaacagctg
     5761 ttatcaaaca gttcaagaac actcaaccac tgccaaacat ctctcaaatg tctgcagttt
     5821 gggatccagc gaaaaatatg ctctttgatg ctgtaagtgg tcaaaaagat gctaaaacag
     5881 ctgctaacga tgctgtaaca ttgatcaaag aaacaatcaa acaaaaattt ggtgaataaa
     5941 aaatttgttc aaggggggtg gaaatcaaat ccccctttga atttatcaat agagacacaa
     6001 ataatttagc tttcttataa aaaagtagta tcctatgaaa ggagttaata tggaaaagca
     6061 acaacctagt aaagcagccc tgctgtctat cattcctggg ttaggacaga tttacaataa
     6121 acaaaaagcc aaaggtttta tcttccttgg tgtaaccatc gtatttgtcc tttacttcct
     6181 agcacttgca acccctgaat tgagcaacct catcactctt ggtgacaaac caggtcgtga
     6241 taattccctc tttatgctga ttcgtggtgc cttccatcta atctttgtaa tcgtttatgt
     6301 actcttttat ttctcaaata tcaaagatgc acatacgatt gcaaaacgca ttaacaatgg
     6361 aattccagtt ccacgcacac tcaaagacat gatcaaaggg atttatgaaa atggcttccc
     6421 ttacctcttg atcattccat cttatgttgc catgaccttc gcgattatct tcccagttat
     6481 cgtaaccttg atgatcgcct ttaccaacta cgacttccaa cacttgccac caaacaagtt
     6541 gttggactgg gttggtttga ccaactttac aaacatttgg agcttgagta ccttccgttc
     6601 tgcctttggt tctgttcttt cttggactat catttgggct ttggcagctt ctactttaca
     6661 aatcgtaatt ggtatcttca cagctatcat tgccaaccaa ccatttatca aaggaaaacg
     6721 tatctttggt gttattttcc ttcttccttg ggctgtccca gccttcatca ctatcttgac
     6781 attctcaaac atgtttaacg atagtgtcgg tgctatcaac actcaagtat tgccaatctt
     6841 ggctaaattc cttcctttcc ttgatggagc tcttattcct tggaaaacag acccaacttg
     6901 gactaagatt gccttgatta tgatgcaagg ttggctcgga ttcccataca tctacgttct
     6961 gaccttgggt atcttgcaat ctattcctaa cgacctttac gaagcagctt atattgacgg
     7021 tgccaacgct tggcaaaaat tccgcaacat cactttccca atgattttgg ctgttgcggc
     7081 acctactttg attagccaat acaccttcaa ctttaacaac ttctctatca tgtacctctt
     7141 caatggtgga ggacctggta gtgtcggagg tggagctggt tcaaccgata tcttgatctc
     7201 atggatctac cgtttgacaa caggtacatc tcctcaatac tcaatggcgg cagctgttac
     7261 cttgattatc tctatcattg tcatctcaat ctctatgatc gcattcaaga aactacacgc
     7321 atttgatatg gaggacgtct aagatgaata actcaattaa actcaaacgt agactgactc
     7381 aaagccttac ttacctttac ctgattggtc tatcaattgt aattatctat ccactgttga
     7441 ttaccattat gtcagccttt aaagcaggta acgtctcagc ctttaaacta gatactaata
     7501 tcgacctcaa ttttgataac tttaaaggcc tcttcactga aaccttgtac ggtacttggt
     7561 acctcaacac tttgattatc gccttaatta ccatggctgt tcaaacaagt atcatcgtac
     7621 ttgctggtta tgcttacagc cgttacaact tcttggctcg taaacaaagt ttggtcttct
     7681 tcttaatcat ccaaatggtg ccaactatgg ccgctttgac agccttcttc gttatggcgc
     7741 ttatgttgaa cgcccttaac cacaactggt tcctcatctt cctctacgtt ggtggtggta
     7801 tcccgatgaa tgcttggctc atgaaaggct acttcgatac agtgccaatg tctttagacg
     7861 aatctgcaaa actagacggt gcaggacact tccgccgctt ctggcaaatt gttctaccac
     7921 ttgttcgccc aatggttgcc gtacaagctc tctgggcctt catgggacct ttcggggact
     7981 acatcctctc tagtttcttg cttcgtgaga aagaatactt tactgttgcc gtaggtctcc
     8041 aaaccttcgt taacaatgcg aaaaacttga agattgccta cttctcagca ggtgctatcc
     8101 tcatcgccct tccaatctgt attctcttct tcttcctaca aaagaacttt gtttcaggac
     8161 ttacaagtgg tggcgacaag ggataattta tccccgccac cctttttcat tttatactct
     8221 tcgaaaatct cttcaaacca cgtcagcttt atctccaacc tcaaagctgt gctttgagca
     8281 acctgtggct agtttgcact ttgattttca ttgattatta gcaattgtca ctgtaaataa
     8341 tatccttgta gcaagcaatt tttctcctag acttgaaata aagcgcattt ctctatataa
     8401 taatactcat atagaaaaca ccttttagaa agatacctat gcttccatat ccattttcct
     8461 atttttcaag tatttggggg tttcgtaagc ccctgtccaa acgtttcgag ctcaactggt
     8521 ttcaacttct ctttaccagt atcttcctta tcagcttgtc tatggtaccc attgctatcc
     8581 aaaacagctc ccaggagacc tatccgctag aaacttttat cgataatgtc tatgaacctc
     8641 tgacagataa ggttgtccag gatctctctg aacatgctac aattgtcgat ggcacattaa
     8701 cttatactgg aacagctagt caagcccctt ctgttgtgat tggtccaagt caaatcaagg
     8761 aattacctaa ggacttgcaa ctgcatttcg atacaaatga actagtcatc agcaaggaaa
     8821 gcaaggaact gacccgcatc tcttaccgag ccattcagac tgagagtttc aaaagcaaag
     8881 acagcttgac ccaagcaatt tctaaagact ggtaccaaca aaatcgtgtc tatatcagcc
     8941 tcttcctagt tctcggtgcg agcttcctct ttggtttgaa tttctttatc gtctctctag
     9001 gagctagtct tctcctttat atcactaaga aatcacgcct cttttcattt agaaccttta
     9061 aagagtgcta tcactttatc ttgaactgtt taggattgcc gactctgatt acacttattt
     9121 tgggattatt tggccaaaat atgacaaccc tgattactgt acaaaatatt ctttttgttc
     9181 tgtatctggt cactatcttt tataaaacac atttccgtga tccaaattac cataaatagg
     9241 agatttttat gcccgttacg attaaagacg tggccaaggc tgctggtgtt tcgccttcaa
     9301 ccgtaacccg tgttattcaa aataaatcaa ccattagcga cgaaacaaaa aaacgcgttc
     9361 gcaaagctat gaaggaactc aactaccacc caaacctcaa cgctcgtagc ttggtaagca
     9421 gctatactca ggttatcgga ttagttcttc ctgatgactc agacgccttc taccagaatc
     9481 ctttctttcc atcggttcta cgtggcatct ctcaagtcgc atctgaaaac cactatgcca
     9541 ttcagatagc aacagggaaa gatgagaagg agcgtctcaa cgctatttca caaatggtct
     9601 acggcaagcg tgtagatggg ctaatctttc tctatgccca agaagaagac cctctcgtaa
     9661 aactcgtcgc agaagaacag ttccccttcc ttatcctagg taaatctcta tctcctttca
     9721 tcccacttgt cgacaacgac aatgttcaag ctggttttga tgcgactgaa tatttcatca
     9781 aaaaaggctg caaacgcatt gcctttatcg gaggaagtaa aaagctcttc gtgaccaaag
     9841 accgtttaac aggctatgaa caggcgctta aacattacaa acttaccact gacaacaatc
     9901 gcatctactt tgccgacgag tttctggaag aaaagggcta taaatttagc aagcgattat
     9961 tcaagcacga tccacaaatt gatgctatca tcacaaccga tagcctccta gctgaaggtg
    10021 tttgtaacta tattgccaaa caccagctgg atgtccctgt tctcagcttt gactcggtta
    10081 atcccaagct caacttggca gcctatgtcg atatcaatag tttagagctt ggtcgtgttt
    10141 cccttgaaac tattctccag attattaatg ataataaaaa caataaacaa atttgttacc
    10201 gtcaattgat cgcccacaaa attatcgaaa aataagagac tgggcaaaaa gtcgttaaaa
    10261 gcaaaaacgc atactatcag gtattgaaaa aacttgatac tatgcgtttt attgtgggaa
    10321 gatttacttc cttttcttct gaaattgagt tgttacccaa gatcttttta tactcaataa
    10381 aaatcaaagt gcaaactagg aagctagccg caggttgctc aaaacactgt tttgaggttg
    10441 tggatagaac tgacgaagtc agctcaaaac atggttttga ggttgcagat agaactgacg
    10501 aagtcagtaa catacctacg gcaaggcgac gctgacgtgg tttgaagaga ttttcgaaga
    10561 gtattaatca ctaattatct atctcaacaa atcttcctag aatatgaaca ttttccgaga
    10621 cagagacaaa ggagcttgga tccacttgtg tcataatctg tttaaattca ttaaactctg
    10681 cacgtgtaat gacagtgatt aaaactgcct ttctctcgtg attataggtt ccttctgcat
    10741 cgtggatcat ggttgctccg cggtgcaatt ttttatggat tttttcaatt accttctctg
    10801 gatgatttgt cacaatcatg gcctgcatac gcttttgctt agtaaagact gcgtctgtca
    10861 cacggctaga gacaaagatg gtaatcatag aataaagagc gtatttccaa ccaaaggtca
    10921 aacctgctat cagcatgata gttccattta ccaagaaaga aatactaccg acattcttac
    10981 ccgttttctt gcgaatagtc aggctgacga tatccgtccc accactggag atattgtttc
    11041 gaagagcaaa accaatcccc aaacccataa caacaccccc aaaaagggaa ttgataatgg
    11101 gatcctccgt caaggttgcc acagggacaa actggataaa gaaggaactc atagataccg
    11161 tgataaaggt aaagacggtg aacttatggc caatctgata ccaagctaag accatcaaag
    11221 ggaagttaat ggcgtagaag cttagcgaaa tcggaatatg aaaaccaaac cagtgattac
    11281 tcaaggcaga gataatctgt gccagacctg ttgcaccact cgaatacaca tgccctggtt
    11341 ggaaaaagaa attaactgct actgctgata aaaaaccata gaccagagag gccgaaatct
    11401 tctcatcata cttttctcga gagatacttt gtaagacacg taaaattttt atctgataag
    11461 caaagcggcg cagataatag cgccaccgct taattcgttt tgtttgtttc atcttcttct
    11521 acttgtaagc tgagttcctc tagttgtttg agagcgactg ttgatggagc ttgtgtcatt
    11581 ggatcagttg ccttgttgtt cttaggaaag gcaatgactt cacggatatt ttcttctcca
    11641 gcaagcaaca tgacaaaacg gtcaagcccg atagccaaac caccgtgtgg tgggaaacca
    11701 tagtccatgg cttcaagaag gaaaccaaac tggtcattgg cttcttcagc tgagaaacca
    11761 agagccttga acatgcgttc ttgaaggtct ttttggttga tacgaaggct accaccacca
    11821 agctcataac cgttcaagac gatatcgtaa gcaatggcac gaaccttagc caaatcacct
    11881 tctaattcgt gagcagtctc ttcctgtgga agtgtgaatg gatggtgggc gctcatgtag
    11941 cggccttctt cttcagacca ttcaaacatc ggccagtcaa ccacccaaag gaagttgaac
    12001 ttatcattat caatcaagcc aagctcttta gcaatacgtc cacgaagggc acccagtgtt
    12061 gcattagcca cttcaagcgt atccgccaca aagagaacca agtccttatc ttcaagagca
    12121 agcgctgttg tcaattcttc ttggatacca gtcaagaact tggcaactgg tccgtttaat
    12181 tctccatcaa ccaccttgac ccaagcaaga cctttggcac catactgttt ggctacttcc
    12241 gtcatcttgt cgatgtcttt acgtgaatag ttgtccgcag ctcctttgac cacaatcgct
    12301 tttacagcaa gtgcttctga aaagacttta aagtctacac ctttgaccac ttctgtcaag
    12361 tcctgaagca acatgtcaaa acgagtatct ggcttgtcag aaccgtaaag agccatagca
    12421 tcatcgtatt tcatacgagg gaacggtagc gttacttcga tgccttttgt ttccttcatc
    12481 acgcgcgcga tcaagccttc tgtaatatct tggatttctt gctcagtaag gaaggacgtt
    12541 tccaagtcga cctgagtaaa ttcaggctgg cggtctccac gcaagtcctc gtcacggaaa
    12601 catttaacga tttggtagta acggtcaaaa ccagcattca tcaagagctg tttcgtgatt
    12661 tgtggacttt gaggaagagc gtaaaaatgc cccttattaa cacgagacgg cactaaataa
    12721 tcacgcgccc cttcaggcgt tgacttagaa aggaatggtg tctccacgtc gataaactcc
    12781 aactcatcca agtagttgcg gatagagtgg gtcaccttgg cacgaagttt aagattttcc
    12841 aacatttctg gacgacgaag gtcaaggtaa cggtaacgca aacgtgtatc gtcatttgcc
    12901 tcaatgccat ccttaatctc aaatggtgtt gtcttagctg tgttaagcac aataagagct
    12961 gtcacgttta actcaaccgc accagttggc aacttatcat tggcttgctc acgcgcagcg
    13021 acctgaccag tcacctcaat aacaaattcg ctacgaaggc tttcagctgt tgccataacc
    13081 tctgcagata ctttttcagg gttgataacc aactgcatga ttccttcacg gtcacgaaga
    13141 tcgataaaga tcaaaccacc aaggtcacga cgacggccaa cccatccttt caaggttatt
    13201 tcttgtccga tgtgttcctc acgaacacga ccagcataca tactacgttt cattatttct
    13261 ctcctctttt attctgttac tattttacca taaaagcgca ggctcttcat gaaaatcatc
    13321 agaaaagttt gccagtcttt aaaagtcagg tgaaagccct aaaaattagc gctaatgctc
    13381 ttcgaaaatc tcttcaaacc acgtcagcgt cgcctt
//
LOCUS       DAVKJJ010000046        13275 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000046 DAVKJJ010000000
VERSION     DAVKJJ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13275
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.046"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            193..375
                     /locus_tag="VW144_001584"
     CDS             193..375
                     /locus_tag="VW144_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9601003.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            320..1169
                     /locus_tag="VW144_001585"
     CDS             join(320..733,735..1169)
                     /locus_tag="VW144_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU9601004.1"
                     /translation="MKKTLTPTKESIDSLRNYEASKQLTDTRFKRLVGVQRTTFKEML
                     AVLKTAYQLKHAKGGRKPKLSLEDLLMATLQYVREYRTYEEIAAVFCIHESNFIRRSQ
                     WVEVTLVQSGVTISRTPLSSEDTVMIDATEVKINRPKKELANDSGKKKCHAMKAQAIV
                     TSQGRIVSLDITVNYCHDMKLFKMSRRNIGQAGKILADSGYQGPMKIYPQAQTPRKSS
                     KLKPLIAEDKAYNHALSKERSKVENIFAKVKTFKIFSTTYRNHRKRFGLRMNLIAGII
                     NHELGF"
     gene            complement(1241..1603)
                     /locus_tag="VW144_001586"
     CDS             complement(1241..1603)
                     /locus_tag="VW144_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601005.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHTFFMDNVEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(1894..3774)
                     /locus_tag="VW144_001587"
     CDS             complement(1894..3774)
                     /locus_tag="VW144_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9601006.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(3768..4637)
                     /locus_tag="VW144_001588"
     CDS             complement(3768..4637)
                     /locus_tag="VW144_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9601007.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLTQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQTCTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(4721..7366)
                     /locus_tag="VW144_001589"
     CDS             complement(4721..7366)
                     /locus_tag="VW144_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEU9601008.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPELL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGEQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKDHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RLYPVYSGLLAPQEIRTEFIKKEEI"
     gene            complement(7457..8737)
                     /locus_tag="VW144_001590"
     CDS             complement(7457..8737)
                     /locus_tag="VW144_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEU9601009.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDEIF
                     VVATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            8908..10992
                     /locus_tag="VW144_001591"
     CDS             8908..10992
                     /locus_tag="VW144_001591"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEU9601010.1"
                     /translation="MKPLLETIDTRFGTASKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGAWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLNQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNPLMLFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALLNLPQEDFDSCKSSAQADWENLLHRFDIIEIGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKLGILFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTSFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLTQYQELGYLSTDHNESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(11032..12711)
                     /locus_tag="VW144_001592"
     CDS             complement(11032..12711)
                     /locus_tag="VW144_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU9601011.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFKKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWANDAALPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSMPLLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLAFQAGETEKSLTLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
BASE COUNT         3722 a         3128 c         2674 g         3751 t
ORIGIN      
        1 atatcatttt taaattttaa gtcaagaaaa attaacccac tttaatttct tttttaacac
       61 gtgttaaaaa agaaattaaa gtgggttaaa aaagaaatta actgagtaaa aactctcgaa
      121 attcaattcg ctatattatc actcaacttg aacaaatacc agaaccacaa aaaacagatg
      181 taaaggaaaa atatggactt aaaatttgaa ggggttgatt tagaatacaa aaaagctaaa
      241 aacaatcttc cagagtcttt ctgggaaacc tactcagcat ttgcaaatac aaatggcggg
      301 aaaataattc tcgggataga tgaaaaaaac attgacacct accaaagagt caatagactc
      361 cctgcgaaat tatgaagcaa gtaaacaatt aactgataca cgatttaagc gtcttgttgg
      421 tgttcagcgt accactttta aagagatgtt agctgtatta aaaacagctt atcaacttaa
      481 acacgcaaaa ggtggacgaa aacctaaatt aagtctagaa gaccttctta tggccactct
      541 tcaatatgtg cgagaatacc gcacttatga agaaattgcg gctgtttttt gtatccacga
      601 aagcaacttt atccgtcgga gccaatgggt tgaagtaact cttgttcaaa gtggtgttac
      661 gatttcaaga actcctctca gttctgagga cacggtaatg attgatgcga cggaagtaaa
      721 aatcaatcgc cctaaaaaaa gaattagcga atgattctgg taaaaagaaa tgccacgcta
      781 tgaaggctca agcgattgtc acaagtcaag ggagaattgt ttctttggat atcactgtga
      841 actattgtca tgatatgaag ttgttcaaaa tgagtcgcag aaatatcgga caagctggta
      901 aaatcttggc tgacagtggt tatcaagggc ccatgaagat atatcctcaa gcacaaactc
      961 cacgtaaatc cagcaaactc aagccgctaa tagctgaaga taaagcctat aaccatgcgc
     1021 tatctaagga gagaagcaag gttgagaaca tctttgccaa agtaaaaacg tttaaaatat
     1081 tttcaacaac ctatcgaaat catcgtaaac gcttcggatt acgaatgaat ttgattgctg
     1141 gtattatcaa tcatgaacta ggattctagt tttgcaggaa gtctattgaa atactaagcc
     1201 tacacacaga ctgattccaa ttgtttttta gatttcaatt ttaccattct tcatagtcaa
     1261 aaccatcata atctggatat tgttctggcg acgttccaac cgaacgtacg agataagctt
     1321 cttcgtcctc tgtctcgatt tctctcagca cttggcattc aaaacgccat tcatcaccga
     1381 agtcgaaaat aaacttaaat ttttgtttga cacttaggct atccagatag acattttctg
     1441 tgtaacgttc ttcaacatcg tcactaacaa agctaagaaa gtaagaatct gcatgactcc
     1501 actcaacatt atccatgaaa aatgtgtgag catgatcatt tgcaaaatca aaactccata
     1561 aaatcacatc tgctagttcc gctaacgttt ttgtttttgg aatctatata tgtcggtagc
     1621 aacctttttt aaatttgacg gaaatgatcc aagcttctac agatgctggc ttttcagctt
     1681 ctatttcttg ttttactaat tttaactgta gcatttgaaa ccagataatt cttctgtttc
     1741 acgcgaaaca ccctgcgcca agcaaggtgt tcttgttttt atactcaatg aaaatcaaag
     1801 agcaaactag gaagctagct gcaggctgct caaaacactg ttttgaggtt gtagatggaa
     1861 gctgacgtgg tttgaagaga ttttcgaaga atattaagtc gtataaatcg tcgacgctgt
     1921 cgcaatggta tgccactggt tggctgttgt agctgcgaaa tccttgtctg cgtagaagtc
     1981 agtaaatggt aggatttcaa cttccagctc gtcgatgcgg tcaagctggc cagccagata
     2041 aacctcgata cggctttctg ctcgtttgat gcgttgcaag agtccgccca tacggatgtc
     2101 aactgtatcc aaaccaaaga ccttgttttc tttcagccat tggtggctaa agagggcatg
     2161 gaagtcttca atttggcttc taagttctgg taattcttgt ctggcgattt gttgtaaact
     2221 ttctttatca tccgcttggt aggcctgacg aatgcgtcgt cccacatcta ctttgctact
     2281 taaaatagca ttcaactggg cctgagtttc aaagagataa gcatagtttc cagctttttc
     2341 tttaatgtta gcaagcgtct cagcagcctg agcgaagtgt ggcttgtcct gttcaggtgt
     2401 catgtgtcgg tcaagtatcg gacagagaac atcctgataa aagacatatc ggttgggatt
     2461 gatgccactg agattacctg gtaggtctgg taaaaggttg gcaaggtcaa tctgcataaa
     2521 atcttcaacc gttagaccag tattggtctt gaaatgcgca gacaaactat ctaggtcatt
     2581 gcgatagctg agttctgccc agatttgcaa gcttggtagg atagagaact gggcagtttc
     2641 accaccattg tctccccaac ccgttacgat gacttcttta atctgattgg cacggcaggc
     2701 tttattagcc tcgatagcca ctagacggct aaaatggttg tgaggtgtaa agccaatcca
     2761 cttccaagct ccccctgcaa atgcaaggtc atggctaatc ttgtgatgat tgcggaaatt
     2821 acggttgtat ttttcctcgc tatcctgata ataatcccag taaaccagag tcacacggtc
     2881 tttgagacgg tctaggtaga cacgagtttc ctctggaatt tccacatcac ggtcgtactg
     2941 gccatccgct gacatgagtt tgaagaacat atcactccac atctggcagt ggaaaccata
     3001 tttgtcagca atatccagca cgcgctccaa gtgttggcac atgaggagac tacgatccac
     3061 aacaccgttc aaaatcaggt agcgtcccaa accaaccaag tgggcttcgt ccatcccgat
     3121 attgaccttg cgagtcttca gtttagacaa cgtggcaaac atgccatcaa tcaggtcata
     3181 aaccttttct tcgccaatga gaagaatgtc ctctacatca cggagctcct gcacttcctt
     3241 gacaccccat ttgacaaagg ccgacaagtg ggccaaggtc tggatgcatg gtacaaaggt
     3301 catgtcaaac tgttgagcat aggcttcgat ttcctgcaac tcctctgctg aataagctcc
     3361 acggaaatag ccaaagtaag gctgcccttc aatctggtaa gtgtcttcca tgtaaagttc
     3421 aaaggttgag tagcccatga gagccaagac ctcaatcatc tgcttggcag aagccacatt
     3481 cagcaccgca tttcgagaac agtcaaccat gtaagccaaa tcttcgtaag ctgcttgttc
     3541 ctcaatctct actttatcag cttctgctag aactgttacc aacaaggata aggcacgata
     3601 aagttggtga ggtttgcggt aggtcaattg atagtgacct tcctcaccct cgatagagat
     3661 agaggcttgg tcagactgag tgacagccac ttccacatct ggtagagaaa tgtgaccttt
     3721 taaaacctca atagcttgcg tttgtttggg actaagtcct gtaaatctta ccattgcttt
     3781 tcctcctgta gccagttgac aagggcaccg tagagattgg catctgcgtg ataggtgcag
     3841 gtctggataa ctggtgcgac cgtgtattct tcgtaggcat cgacaaagtc ttcaacagcc
     3901 ttcttaacac cttggataaa atctggattt tgactgatag agccacccag actgatgaca
     3961 tctggatcga tcagatactg gatattgagc aagccttgcg tcagattgcg gttcatgcgc
     4021 tcaatggctt cttgacaaag agcattacca gctgcggctt cttggtaaat cttgcgaccg
     4081 tcccaatcag tatgaccaga tttttcaatc acgtatcgta ccatattccc agttgatgct
     4141 agttgcgacc agttattaag tttttcagca ggggcaaggg ttgtcatgta gccaaattct
     4201 ccacccagac cgtggcgacc tcgatgaagt ctaccattga taatcatggc tccgccaatc
     4261 cctgtcccaa tcacgacaca ggctgcattt tcaagctctg gatgagctag tagttcactg
     4321 agtccaacgc agttggcatc attttctaaa tggacaggta gctgataaga gctaagcgcc
     4381 tcataccaag aaaagccatg gatgtagggc accgcactga agccatcaat cacacctgtc
     4441 tcttgattga ccgcacctgg aacgctcata gcaatcccac tgtaatcctg ttctgacaag
     4501 cgttgatcta gccacgctag taaatcctcc aagttttcag gcgttgaaat acttgtctta
     4561 tccagtattt tcccatcagg agtcagactg gcaaacttaa tcccagtccc tccgatatca
     4621 atcgttgcaa tggtcattgt ttttaccata aaaaggggcg aatcagcagt tagttcttac
     4681 tttttcgccc atcctttctt ttgatgttta ctttttgaaa ttaaatttct tcttttttga
     4741 tgaattctgt acgaatctct tgtggagcca atagtcctga ataaactgga tatagtcgct
     4801 caagtaagtc aagaatggtt tgttgatgtt ctgatatgcg cacattttct tgactcatat
     4861 tatagtaacg gaggacatag ccttcttcat tttccgccac tttaaaggct gttggacaaa
     4921 cttgcggtat gctgagaaca gaatggctca agaggctacc agtcgcaacc acgcttcctt
     4981 cctgtctagc aagctgaagg ctggtaaacg gtgtctgcaa ggctttggca cgacgatagg
     5041 ctgagaagcg ttcttgggct tggtggcatt caagtgcaaa ctcgacttca aactcccgca
     5101 agcattgtgc ttctggcgtt gggaagtagc cccagtcacc tagctcacct gatgcacgca
     5161 aaatggtcac ggcaatggtg ttatccccaa ggatttcgta ttcattcaat cccttgttgg
     5221 atacagtcac ccctttttca tcgtcataca gactgacaaa agcttgttgg tgttgaggat
     5281 tttcagggtt ttcccatgaa gcagctggtt tgtttggtcg tgtcaccacc tcatagatac
     5341 tttcagaatc attgcttgga cgcgtgttat gagtcttgac caagagacgg atacggtgat
     5401 ccttggcagt gttagtaaag cgagtcttga agcggatttg tggattgtca acgaagacag
     5461 tcaactcagt ttccagagga atgtttgtca attcttctga ccgtccagcc tcacgcttca
     5521 taaactcgat gataccttgt tgctcttctt ctagcttttc atccgcactg acaggcacgg
     5581 tcaattcatg tttgagcaag attttagcat agcgatctgt gttttccaag acctcgtggt
     5641 ccttaagctc tgcaaagatt ggctctgttc cttttggttg gaaatagata tactcatttc
     5701 cgatgtcccc acggtcttca aagcggataa agtcttcata ggcttcgtga gttgtcttat
     5761 catagactgt gatgttgtca tccacactca ccgttacgaa tggtgtatca atcactccgt
     5821 tttggtaaat accctcacgg tgttcttgtt ctccttccag caattggaag gttgtccaag
     5881 aaagcggtgc taggtgaact ggaatggtca cgcgcacttg tcgagcaata cgagcttggc
     5941 ggaacttgtc ttttggtaaa tcgtattcaa aattagctcc gaggtcttcg attgtagcct
     6001 ctacaggacg accatccaag tcctccacac ggtaacttgg caagataaga gcagccatct
     6061 ttttgtagcc ttctgttggg tgcaattcct tgaaatcaca agtcgccaca tcaatcactg
     6121 tgctgacagt atcgacctta tcatgcaagc ctgtgttaat gacagtaaag agatagtcac
     6181 tttgagcctt atccgtagca attttaccct tccactcgtt gagcaaatta cttttaacaa
     6241 agtttcctac ttggttgacc ttggcaaaac gcgtttccat ctcgcggtga acttcgtcca
     6301 cgctacagcc acagatacta tcatgtggcg cattctgcaa aagtgttttc caagcatagg
     6361 tcaactggtc cttgtggttg tgtccaccag tgataatagt caagggttct accacttgct
     6421 ctaggaggtt gctattttct tggaaggctt gttttaggta aatgcgggat gaagaagtgt
     6481 tggcaagtgt gtaccagcca tctgtttcct gactggtcaa ctcacctgta acagttgata
     6541 agagttcagg aagcgcacct tctacagctt gaacatattc atcaaaagaa ctatgaacaa
     6601 agattacatc cgggaagagt tcatttgcca cacgaatggc ttcgctcaga tttttctgta
     6661 caggctggtg gtcacagccg ttcatcatca accattggtt ggtcgaagcg taggcacgca
     6721 catctgacaa tttttgtttc cagaaggtca aggcctcatc tttgtcaact gggatttcat
     6781 tcccattact gtaccagtta gcaaagagaa tacctaaaac acgactacca tccacaccct
     6841 gccagtacat ttctgaaaac tgggatgtaa actgctcatc ttcaaggact tggttgtcaa
     6901 atccaatcgg cttcacacca cgaccaaagg ccgccacgtg aatgcctgat ttttgaagaa
     6961 tttgaggcgc ttgtcccata tttccaaagg tatctggaaa gtagccaatc tgggttgatt
     7021 taccccattt ggcagcttct tgttgaccaa tcaaggtatt gcggacattg gcttcactgg
     7081 agatcaagta gtcatcctgc aagatgtaaa agggaccaat tttaagtttg ccctcgtcaa
     7141 tgtagcgttg gaccttgtcg cgattttcag ggcgaatttg taagtagtcg tcaaggacaa
     7201 tagtttgtcc atccaagtgg aaactcttga actcagggtc attttcaaag agatcaaaga
     7261 gattgtcaaa caattccacc aactgcatac gatggctttc aaaaggcaag taccactcac
     7321 ggtcccagtg actatgtgag ataatatgta caacaacatt ttccatgaag taaaacctca
     7381 ttctaaattt aaattttaac gttttaaatg taaacttgtg attctgataa gaatcggttg
     7441 agctaaagcg agccccttag cgaatatcca agtaatccaa gaccaactca cagaacatca
     7501 tgttagccca ggagaaccat tcacgagagt agagggtcgg atcatctacg tggaagcttt
     7561 cgtgcatgac acctgtacca ccatcgcagg caaccagctg atccagcaag aattttttct
     7621 ctgccttatc tcttgttgtc aatccttgga tagaaagggc gattggccag atatagcgat
     7681 agaaggtatg agaacttccg agaccgctag cgtattctcc ttggtagaag tatggatttt
     7741 cagagctcaa aatggtacgg cgagtagctt gatacacttc atcatcgacc gaacagtagc
     7801 ccagataggg cgcagctagt agacttggta catttggatc atccatgatg ctggcatttc
     7861 ctaggccatc cacttcaaaa gcgtaaatct tttcgccctt gctgttggtg gtgtaagcgt
     7921 agttttcgat tccttcttgg atttcatcct gaagacgctt ggcatcagca ataacactct
     7981 ggctatcagc taggtttaat gctgcgaaga tttcttgcac ataacccaag actactacag
     8041 caaacatatt tgacggaatc aagtaactat actggcaaca gtcatcactc ggacgaaaag
     8101 ctgaccaagt catacctgtc actgcaaagt caggtccaaa gccatcattt accaaggtgt
     8161 cttccttacg gtccgtatct cggacaaaac gataaggaga gttcttgtgg tcttgttcaa
     8221 ctgtccatag atggagaatt tccttagtcg ctacgacaaa aatctcatca aactgactag
     8281 tctcgccagt ctctttccag aggagataag ccaactgcaa aggatagcaa agtgaatcca
     8341 cctcatactt gcgctcccag atccagccgt taaggtctgt gtgatcagtc tcgtggtgcc
     8401 ctttccagtt ctcctcaatg ttgaaggagt tagcataggg atccttgagt accaaggtca
     8461 tctgacgttt gaccaaacct gcaatggtct gacgcaggag ggcatctctt ttagctacat
     8521 gaaggtaggg tctgagttgg gctgtcgaat ctcgaagcca catggcagga atatccccag
     8581 tcaagacaaa agttgaacca tcttctaaga tttcaaccgt attgtccaag gtatcggtgt
     8641 agcaacgctc gaaaacatcc acccactctg gatggtcctt agcccgctct gctacttcat
     8701 ctagccattc tctaacaatt tctttcgaat aaatcatcgt gcaattccct ctttcaaaca
     8761 agtttcattt tcagtatata gcttttgaga cagaaaatac atacacaaat gatagtcatt
     8821 tttctacaaa atagttatct ttaaaactcc ttttctaaag gccctctttt tctgatataa
     8881 tgtagaaaaa cagctaaagg aaagactatg aaaccactac ttgaaaccat cgatacccgc
     8941 tttggtactg ctagcaagca tgccttctct cggggaaata ccctgccata cacaggcgtg
     9001 ccttttggga tgaattattt tgtgccccag accagtgacc aggacggagc ttggtttttt
     9061 gatccgcatc tgcctatctt tcaggggatt cgattaactc accagcccag tccttggatt
     9121 ggggactact cttggctcct tctgacacct gtcaccagtc aactgggtgg agacagcctc
     9181 tttcaccgcc agtcttctta tgatatagat aaagcttgct tccagcctca ctatttgaag
     9241 ctcttttccc tgcgctatca aattgaaacc cagctcacac cgacttgcta tggtgcttct
     9301 attcgtttga atcaaaagca aggcaaagcc ctctccctct atcttcacgc agcagatgaa
     9361 ctgaccgtag agcaagtaga taagcggact ctggctctca gacaagaagg caaaacagag
     9421 accaataaaa atccactgat gctcttcact gccctgcaaa tgaacacgga tattcttgct
     9481 atcagccaag aagctggaga ctggcgaatt gacttagcaa gtagtcaaac cgagatgcaa
     9541 ctagccactt ctttcatctc tccttctcaa gcactactta atctacctca agaagatttt
     9601 gatagctgta aatcaagtgc ccaagcggat tgggaaaatc tcctccatcg ttttgacatt
     9661 atagagatag gagaggctga ccgaaccttc tttgaccact gtctctacag actcttcctc
     9721 ttcccacaga ctttttatga ggttgatgag tcagggcaag ccatccacat ggatctggct
     9781 actggtactg tcaagcttgg tatcctcttt agcaacaatg gtttctggga taccttccgc
     9841 accaccttcc ccctctttgc ccttatcata ccggagcact atcaacgctt tttagaaggt
     9901 ttcctcaata gctaccgcga tactagtttc cttccaaaat ggctggctcc agatgaacgg
     9961 ggtatgatgc caggtacact tttagacggt attatcgcag atagcgcctg caaggacatg
    10021 gcccccgacc tagaaggaga actcttccaa gccatgctcg aaacagccag caaagccgac
    10081 cctctcggca tcaatggccg ccacggacta acccaatacc aagaactagg ctacctctct
    10141 accgaccaca acgaaagtgt tagccatact ctagactatg cctatagtga tttttgtatc
    10201 gccagctgtg ccaaaaaact agagaacata gaaatcgctg aaacctacaa ggctgcttca
    10261 caaaattacc gccagctatt tgacgctgag acaggttaca tgcgagcacg agacaatcaa
    10321 ggaaactttc accctgactt ctctccttat agttgggggc gagactacgc tgaatgctct
    10381 gccattcaag ctactttagg cgttctccac gacatccctg gcttaatcca acttatgggt
    10441 ggaaaagaaa cctttagcaa ctatcttttg aaagcctgtc aagatgctcc cctctttgaa
    10501 acaacaggct atggttacga aattcacgaa atgagcgaga tggctactgc tccttttgga
    10561 caaatagcca tttccaacca gcctagtttc cacattcctt atctcttccg ctacagcgat
    10621 taccctgact acactgccct tcttatcaag acgctccgtc agaaagcttt tcacccaagt
    10681 tgggaagcct atcctggaga tgaagacaat ggtagtctct ctgcttggta catctggtca
    10741 gctctcggat tttatccaac ctgtccagga aaaccaagct atgacctcgg aatccctctc
    10801 tttgaccatc tacgagtcta cctcgctaaa gaagataaat ggctggatat ccatactaaa
    10861 caaaaccaca accattttaa ctttgtcaaa gaatgccgac tggacaaaac actcgtatca
    10921 actattcaac accaagacct cttaaaagct gaacaactaa ctttcaccct cagctggtta
    10981 ccaagtcact aataaccaaa agaacctttg catcgcgcaa aggttcttct tttatgaaac
    11041 ttgaacttga agttgatcca caagctgacc atccaccgtt aggttcagat agaaatccaa
    11101 ggttttatct cctgcgaaat acaaggttgg tagcgtcaga cttttttcag tctcaccagc
    11161 ttgaaacgca agaacttgaa tctcatcctg ataggcgaca ccatggacac ctgtccccgg
    11221 ttgaatcgaa atcttagcct ctaaagagga gctactttca ctacgttcaa tcctaaaatg
    11281 aagagtctct ccctttgcca cagctaggct tttttctgca aatgctagtt cctgaacaac
    11341 ttcttttttt gataacccag gagttttata aagggaaatc ttggtcagta gaggcataga
    11401 ttgtgcttcg gtaatcatca cgcgcacctt ctgtgcctct actagcggac ctcgcaagag
    11461 gcgcttatga ccaactgtga agcccctgcc aaattcctgc cagacaccat ccacctctac
    11521 ttgcacatgg aaagcagcga ttcgttgccc tagcttcaaa tcttctctta actcaattac
    11581 atcaaaagtt ttaggagaac ctaagtcgag ttctaactgg atgggcaagg ctgcatcgtt
    11641 tgcccaagag ctggtctcaa ggccgtctgt caaatggcga caagcaaagt ctgcggaaag
    11701 agctggacca gatacctcag ctcccagagc caaatcttct ttatagagct cattgcgata
    11761 ggtcgcaaat tcataaagtc gttcaatatc ctttgcatca aagagcccag cttgattcgg
    11821 cggaatatta agcaagagtg gagttcctcg ccctactgag tgaaagtaga tttcgaccaa
    11881 ctcctcgaga gacttaggat cctgatcctc atggtagaac cagcctggac ggatggaaac
    11941 atctgcctct ccgattgaaa aaatcgtgcc cgagggatcc ccgtgctgaa gatagttcag
    12001 ctctgcttct gttcctagtt tatcaggatt caccttttgc cacagtggat cacctgcata
    12061 ccctcgttca ttgccaatcc agcggatact ggtgccttct gttgaaaaaa tcaagcaatc
    12121 gccctgcagg tcacgaatgg tttcaaacca ttttttaaat tcataattaa ccttttgcgc
    12181 accctctcct ctggcaccat ccatccaaac ctcagcgaac ttaccagcat tcccatagtt
    12241 aggatttgat aagatttcct tcaactgagc cagataatag gcattgtagt ccgcttctcg
    12301 gtccacatga tagaggggac tatgggcatc ccacggtgac aggtagaccc ccatatccat
    12361 atcaaactct gtggcagctt gggatacttc aaggagcaag tcgccctttc ctctcctcca
    12421 aggactgacc ttaaccgaat aatctgtgtg agctgtcgga taaaggacaa agccatcgtg
    12481 gtgcttgacc accaaaatca actttttgaa gcccgtttcc ttgagcacac gaacccactc
    12541 acgcgcatcc aactgactcg ggttaaagcg ctcaggatcc tcctgtccag tcccccattc
    12601 ttggtcataa aaggtattag gaccgaagtg gataaaagct gctagttcat ctcccagata
    12661 agctagctga gtctgacttg gtaacggtcc atgcggtttg attttcttca tcgttatata
    12721 tctccttctt tagcaacttc tcttgttcac aaggttatcc acaggttgtg gataataaac
    12781 catgatctaa cagccagctc tttccaactc caactggtag gctcaggatt tttctcctca
    12841 atattccctc ttcaaacagc tctgtcatat ggttctgcta acacctctca gaatgctact
    12901 ctccataatt attctcaggc ttctttcagg tcattgtgtg aagaccattc actctccaga
    12961 cttttccaca gattgtgaat aagtgattat tctaagctat cacctcgtcg actataacaa
    13021 aaaataaaaa gagggtcaat aagataatta tctcttccta aatactaaga aaagattcta
    13081 taatttgtac aattgaacaa gaaatgcttc tccaaaatct atcttcaaaa gaatgttgca
    13141 tctttttatc ctttcacaca aacatttctc tttatacaga tccaagtgat aaccagatga
    13201 taattggaca ctttttggtc atttgtatat tttttgaaaa attttttaaa aaaattttat
    13261 atccttttta tagaa
//
LOCUS       DAVKJJ010000047        13237 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000047 DAVKJJ010000000
VERSION     DAVKJJ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13237
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.047"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            61..5631
                     /gene="zmpC"
                     /locus_tag="VW144_001593"
     CDS             61..5631
                     /gene="zmpC"
                     /locus_tag="VW144_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease ZmpC"
                     /protein_id="HEU9601012.1"
                     /translation="MSRKSIGEKRHSFSMRKLSVGLVSVTVSSFFLMSQGIQSVSADN
                     MESPIHYKYMTEGKLTDEEKSLLVEALPQLAEESDDTYYLVYRSQQFLPNTGFNPTVG
                     TFLFTAGLSLLVLLVSKRENGKKRLVHFLLLTSMGVQLLPASAFGLTSQILSAYNSQL
                     SIGVGEHLPEPLKIEGYQYIGYIKTKKQDNTELSRTVDGKYSAQRDSQPNSTKTSDVV
                     HSADLEWNQGQGKVSLQGEASGDDGLSEKSSIAADNLSSNDSFASQVEQNPDHKGESV
                     VRPTVPEQGNPVSATTVQSAEEEVLATTNDRPEYKLPLETKGTQEPGHEGEAAVREDL
                     PVYTKPLETKGTQGPGHEGEAAVREEEPAYTEPLATKGTQEPGHEGKATVREETLEYT
                     EPVATKGTQEPEHEGEAAVEEELPALEVTTRNRTEIQNIPYTTEEIQDPTLLKNRRKI
                     ERQGQAGTRTIQYEDYIVNGNVVETKEVSRTEVAPVNEVVKVGTLVKVKPTVEITNLT
                     KVENKKSITVSYNLIDTTSAYVSAKTQVFHGDKLVKEVDIENPAKEQVISGLDYYTPY
                     TVKTHLTYNLGENNEENTETSTQDFQLEYKKIEIKDIDSVELYGKENDRYRRYLSLSE
                     APTDTAKYFVKVKSDRFKEMYLPVKSITENTDGTYKVTVAVDQLVEEGTDGYKDDYTF
                     TVAKSKAEQPGVYTSFKQLVTAMQSNLSGVYTLASDMTADEVSLGDKQTSYLTGAFTG
                     SLIGSDGTKSYAIYDLKKPLFDTLNGATVRDLDIKTVSADSKENVAALAKAANSANIN
                     NVAVEGKISGAKSVAGLVASATNTVIENSSFTGKLIANHQDSNKNDTGGIVGNITGNS
                     SRVNKVRVDALISTNARNNNQTAGGIVGRLENGALISNSVATGEIRNGQGYSRVGGIV
                     GSTWQNGRVNNVVSNVDVGDGYVITGDQYAAADVKNASTSVDNRKADRFATKLSKDQI
                     DAKVADYGITVTLDDTGQDLKRNLREVDYTRLNKAEAERKVAYSNIEKLMPFYNKDLV
                     VHYGNKVATTDKLYTTELLDVVPMKDDEVVTDINNKKNSINKVMLHFKDNTVEYLDVT
                     FKENFINSQVIEYNVTGKEYIFTPEAFVSDYTAITNNVLSDLQNVTLNSEATKKVLGA
                     ANDAALDNLYLDRQFEEVKANIAEHLRKVLAMDKSINTTGDGVVEYVSEKIKNNKEAF
                     MLGLTYMNRWYDINYGKMNTKDLSTYKFDFNGNNETSTLDTIVALGNSGLDNLRASNT
                     VGLYANKLASVKGEDSVFDFVEAYRKLFLPNKTNNEWFKENTKAYIVEMKSDIAEVRE
                     KQESPTADRKYSLGVYDRISAPSWGHKSMLLPLLTLPEESVYISSNMSTLAFGSYERY
                     RDSVDGVILSGDALRTYVRNRVDIAAKRHRDHYDIWYNLLDSASKEKLFRSVIVYDGF
                     NVKDETGRTYWARLTDKNIGSIKEFFGPVGKWYEYNSSAGAYANGSLTHFVLDRLLDA
                     YGTSVYTHEMVHNSDSAIYFEGNGRREGLGAELYALGLLQSVDSVNSHILALNTLYKA
                     EKDDLNRLHTYNPVERFDSDEALQSYMHGSYDVMYTLDAMEAKAILAQNNDVKKKWFR
                     KIENYYVRDTRHNKDTHAGNKVRPLTDEEVANLTSLNSLIDNDIINRRSYDDNREYKR
                     NGYYTISMFSPVYAALSNSKGAPGDIMFRKIAYELLAEKGYHKGFLPYVSNQYGAEAF
                     ASGSKTFSSWHGRDVALVTDDLVFKKVFNGEYSSWADFKKAMFKQRIDKQDNLKPITI
                     QYELGNPNSTKEVTITTAAQMQQLINEAAAKDITNIDRATSHTPASWVHLLKQKIYNA
                     YLRTTDDFRNSIYK"
     gene            5987..6841
                     /locus_tag="VW144_001594"
     CDS             5987..6841
                     /locus_tag="VW144_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9601013.1"
                     /translation="MTYEYQSHIYLAEAVLNVKDLVSQTVFYQQIIGLEILSQTDTEV
                     VLGLGGKALVHLIQAQEGGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEALYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEVLAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSRQFYQTVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLDPRKQVLPGLAYYVIEVAHKEELLTIAQRAQEVDAPIKWMTSIQLEITDSDGI
                     VTRIRLAR"
     gene            6947..7504
                     /locus_tag="VW144_001595"
     CDS             6947..7504
                     /locus_tag="VW144_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEU9601014.1"
                     /translation="MTSEYQKMIAGEFYRPSDPELRALAQASRQKQAAFNKEENPLKE
                     AEIIKTWFASTGKNLYINTRLMVDYGVNIHLGENFYSNWNLTMLDICPIRIGDNAMIG
                     PNCQFLTPLHPLDPQERNSGIEYGKPITIGDNFWTGGGVIVLPGVTLGNNVVAGAGAV
                     ITKSFGDNVVLAGNPARVIKEIPVK"
     gene            7721..8485
                     /locus_tag="VW144_001596"
     CDS             7721..8485
                     /locus_tag="VW144_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEU9601015.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVVNYKDEEICLVQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPSRYMEMQPEAIAAIERVLD
                     DRGIPYEEVMTWSTDGFYRETAEKVAYRKEEDCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            9098..10477
                     /locus_tag="VW144_001597"
     CDS             9098..10477
                     /locus_tag="VW144_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9601016.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            10491..11156
                     /locus_tag="VW144_001598"
     CDS             10491..11156
                     /locus_tag="VW144_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU9601017.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            11291..11494
                     /locus_tag="VW144_001599"
     CDS             11291..11494
                     /locus_tag="VW144_001599"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601018.1"
                     /translation="MKIKDQTRKLATGCLKHCFEVVDRTDEVSSKHCFEVVDRTDEVS
                     SKHCFEVADRTDEVSNIYTARRC"
     gene            11556..11729
                     /locus_tag="VW144_001600"
     CDS             11556..11729
                     /locus_tag="VW144_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU9601019.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGWDQVGNFSKGQYIMMHS
                     LIYRTDLLRASQF"
     gene            11796..12206
                     /locus_tag="VW144_001601"
     CDS             11796..12206
                     /locus_tag="VW144_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU9601020.1"
                     /translation="MYYLPVDFYRYLIGREGQSVNEQVMIKCIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            12322..>13237
                     /gene="pavB"
                     /locus_tag="VW144_001602"
     CDS             12322..>13237
                     /gene="pavB"
                     /locus_tag="VW144_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEU9601021.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAV"
BASE COUNT         4237 a         2302 c         2949 g         3749 t
ORIGIN      
        1 tatactttta tgctataaaa tatagattga tataaagaat atagaaaaag cgaggttaat
       61 atgagccgaa aaagcattgg tgagaaacgc catagtttct cgatgagaaa gttgtcagtg
      121 ggattggtat cagttactgt atctagtttc tttttgatga gtcaagggat tcaatcggta
      181 tcggccgata atatggaaag tccaattcat tataagtata tgaccgaggg taaattgaca
      241 gacgaggaaa aatccttgct ggtagaggcc cttccacaac tggctgaaga atcagatgat
      301 acttattact tggtttatag atctcaacag tttttaccga atacaggttt taacccaact
      361 gttggtactt tcctttttac tgcaggattg agcttgttag ttttattggt ttctaaaagg
      421 gaaaatggaa agaaacgact tgttcatttt ctgctgttga ctagcatggg agttcaattg
      481 ttgccggcca gtgcttttgg gttgaccagc cagattttat ctgcctataa tagtcagctt
      541 tctatcggag tcggggaaca tttaccagag cctctgaaaa tcgaaggtta tcaatatatt
      601 ggttatatca aaactaagaa acaggataat acagagcttt caaggacagt tgatgggaaa
      661 tactctgctc aaagagatag tcaaccaaac tctacaaaaa catcagatgt agttcattca
      721 gctgatttag aatggaacca aggacagggg aaggttagtt tacaaggtga agcatcaggg
      781 gatgatggac tttcagaaaa atcttctata gcagcagaca atctatcttc taatgattca
      841 ttcgcaagtc aagttgagca gaatccggat cacaaaggag aatctgtagt tcgaccaaca
      901 gtgccagaac aaggaaatcc tgtgtctgct acaacggtgc agagtgcgga agaggaagta
      961 ttggcgacga caaatgatcg accagagtat aaacttccat tggaaaccaa aggcacgcaa
     1021 gaacccggtc atgagggtga agccgcagtc cgtgaagact taccagtcta cactaagcca
     1081 ctagaaacca aaggtacaca aggacccgga catgaaggtg aagctgcagt tcgcgaggaa
     1141 gaaccagctt acacagaacc gttagcaacg aaaggcacgc aagagccagg tcatgagggc
     1201 aaagctacag tccgcgaaga gactctagag tacacggaac cggtagcgac aaaaggcaca
     1261 caagaacccg aacatgaggg cgaagcggca gtagaagaag aacttccggc tttagaggtc
     1321 actacacgaa atagaacgga aatccagaat attccttata caacagaaga aattcaggat
     1381 ccaacacttc tgaaaaatcg tcgtaagatt gaacgacaag ggcaagcagg gacacgtaca
     1441 attcaatatg aagactacat cgtaaatggt aatgtcgtag aaactaaaga agtgtcacga
     1501 actgaagtag ctccggtcaa cgaagtcgtt aaagtaggaa cacttgtgaa agttaaacct
     1561 acagtagaaa ttacaaactt aacaaaagtt gagaacaaaa aatctataac tgtaagttat
     1621 aacttaatag acactacctc agcatatgtt tctgcaaaaa cgcaagtttt ccatggagac
     1681 aagctagtta aagaggtgga tatagaaaat cctgccaaag agcaagtaat atcaggttta
     1741 gattactaca caccgtatac agttaaaaca cacctaactt ataatttggg tgaaaataat
     1801 gaggaaaata ctgaaacatc aactcaagat ttccaattag agtataagaa aatagagatt
     1861 aaagatattg attcagtaga attatacggt aaagaaaatg atcgttatcg tagatattta
     1921 agtctaagtg aagcgccgac tgatacggct aaatactttg taaaagtgaa atcagatcgc
     1981 ttcaaagaaa tgtacctacc tgtaaaatct attacagaaa atacggatgg aacgtataaa
     2041 gtgacggtag ccgttgatca acttgtcgaa gaaggtacag acggttacaa agatgattac
     2101 acatttactg tagctaaatc taaagcagag caaccaggag tttacacatc ctttaaacag
     2161 ctggtaacag ccatgcaaag caatctgtct ggtgtctata cattggcttc agatatgacc
     2221 gcagatgagg tgagcttagg cgataagcag acaagttatc tcacaggtgc atttacaggg
     2281 agcttgatcg gttctgatgg aacaaaatcg tatgccattt atgatttgaa aaaaccatta
     2341 tttgatacat taaatggtgc tacagttaga gatttggata ttaaaactgt ttctgctgat
     2401 agtaaagaaa atgtcgcagc gctggcgaag gcagcgaata gcgcgaatat taataatgtt
     2461 gcagtagaag gaaaaatctc aggtgcgaaa tctgttgcgg gattagtagc gagcgcaaca
     2521 aatacagtga tagaaaacag ctcgtttaca gggaaactta tcgcaaatca ccaggacagt
     2581 aataaaaatg atactggagg aatagtaggt aatataacag gaaatagttc gagagttaat
     2641 aaagttaggg tagatgcctt aatctctact aatgcacgca ataataacca aacagctgga
     2701 gggatagtag gtagattaga aaatggtgca ttgatatcta attcggttgc tactggagaa
     2761 atacgaaatg gtcaaggata ttctagagtc ggaggaatag taggatctac gtggcaaaac
     2821 ggtcgagtaa ataatgttgt gagtaacgta gatgttggag atggttatgt tatcaccggt
     2881 gatcaatacg cagcagcaga tgtgaaaaat gcaagtacat cagttgataa tagaaaagca
     2941 gacagattcg ctacaaaatt atcaaaagac caaatagacg cgaaagttgc tgattatgga
     3001 atcacagtaa ctcttgatga tactgggcaa gatttaaaac gtaatctaag agaagttgat
     3061 tatacaagac taaataaagc agaagctgaa agaaaagtag cttatagcaa catagaaaaa
     3121 ctgatgccat tctacaataa agacctagta gttcactatg gtaacaaagt agcgacaaca
     3181 gataaacttt acactacaga attgttagat gttgtgccga tgaaagatga tgaagtagta
     3241 acggatatta ataataagaa aaattcaata aataaagtta tgttacattt caaagataat
     3301 acagtagaat acctagatgt aacattcaaa gaaaacttca taaacagtca agtaatcgaa
     3361 tacaatgtta caggaaaaga atatatattc acaccagaag catttgtttc agactataca
     3421 gcgataacga ataacgtact aagcgacttg caaaatgtaa cacttaactc agaagctact
     3481 aaaaaagtac taggagcagc gaatgatgca gccttagata acctatactt agatagacaa
     3541 tttgaagaag ttaaagctaa tatagcagaa cacctaagaa aagtattagc gatggataaa
     3601 tcaatcaata ctacaggaga cggtgtagtt gaatacgtaa gtgagaaaat caaaaataac
     3661 aaagaagcat ttatgctagg tcttacttat atgaaccgtt ggtacgatat taattatggt
     3721 aaaatgaata caaaagattt atctacgtac aagtttgact ttaacggaaa taatgagact
     3781 tcaacgttgg atactattgt cgcattagga aatagtggac tagataacct gagagcttca
     3841 aatactgtag gtttatatgc gaataaactt gcatcggtaa aaggagaaga ttcagtcttt
     3901 gacttcgtag aagcgtatag aaaactgttc ttaccaaaca aaacaaataa cgagtggttt
     3961 aaagaaaata caaaggcata tatagtcgaa atgaagtctg atattgcaga agtacgagaa
     4021 aaacaagaat caccaacagc cgatagaaaa tattcattag gagtttacga tagaatatca
     4081 gcaccaagtt gggggcataa gagtatgtta ttaccactac taactttacc tgaagaatct
     4141 gtgtatattt catcgaatat gtctacactt gcattcggtt cgtatgaaag atatcgtgat
     4201 agtgtggatg gagttattct ttcaggagat gctttacgaa cttatgtaag aaatagagtt
     4261 gatatagcag cgaaaaggca tagagaccat tatgatattt ggtacaatct tcttgacagt
     4321 gcttcaaaag aaaaactttt ccgttctgtg atagtttatg atggattcaa tgtaaaagat
     4381 gagacaggaa gaacttattg ggcaaggtta acggataaaa acatcggctc tattaaagaa
     4441 ttcttcggac ctgttgggaa atggtatgag tataatagta gtgcaggagc gtatgcgaat
     4501 ggaagtttaa cgcactttgt gttagataga ttattagatg cttatggaac gtcggtttat
     4561 actcatgaaa tggttcataa ttctgattct gcaatctact ttgaaggaaa tggtagacgt
     4621 gaaggattgg gagcggagtt atacgcactt ggtttactgc aatctgtaga tagtgtaaat
     4681 tctcatattt tagctttaaa tacgttatat aaagcagaaa aagatgattt gaatagattg
     4741 catacatata atccggtgga acgtttcgat tcggatgagg cgcttcaaag ttatatgcat
     4801 ggatcatatg atgtaatgta tacacttgat gcgatggaag caaaagcgat attagctcaa
     4861 aataatgatg ttaagaaaaa atggtttaga aaaatagaaa attattacgt tcgtgatact
     4921 agacataata aagatacaca tgcaggaaat aaagtccgtc cattaacaga tgaagaagta
     4981 gctaacttaa catcgttaaa ctcattaatc gacaacgaca tcataaatag acgtagctat
     5041 gatgataata gagaatataa acgaaatggc tactatacta taagtatgtt ctctcctgta
     5101 tacgcagcgc taagcaattc gaaaggtgct cctggagata ttatgtttag aaaaatagct
     5161 tatgaattac ttgcggaaaa aggttatcac aaaggattcc taccttatgt ttctaatcag
     5221 tacggagcag aagcatttgc cagcggaagc aaaacattct catcatggca tggaagagat
     5281 gttgctttag tgacagatga tttagtattt aagaaagtat tcaatggtga gtactcatca
     5341 tgggctgatt tcaaaaaagc aatgtttaaa caacgtatag ataaacaaga taatctgaaa
     5401 ccaataacaa ttcaatacga attaggtaat cctaatagta caaaagaagt aactataaca
     5461 acggctgcac aaatgcaaca attaattaat gaagcggctg cgaaagatat tactaatata
     5521 gatcgtgcaa cgagtcatac cccagcaagt tgggtgcatt tattaaaaca aaaaatctat
     5581 aatgcatatc ttcgcactac agatgacttt agaaattcta tatataaata agattgtaga
     5641 gtttcattgt tgagtagtgt ttcttgtaag gatgaggagt cagatgacaa atcgactcct
     5701 ttttcttatg gatcgatgta gagatttgat tgaatgcaga ttgcaggaat catcttcaac
     5761 tcatcaacga ccaatggtga caaggtggat ttcaatccca cagaaaatgt tgatttgaga
     5821 aataactttg ctagtctagt aaaataaata caaaacaatc ctagaagatt ttttctggga
     5881 ttgttttttg ctgagtggga tgcttcaagt tgtctggctt gactttcttg agggaagtta
     5941 tataatagtt gtaataatta aagttacaat aaaagaggaa atatttatga catatgaata
     6001 ccagagccac atttatttag cagaggcagt tttaaatgtg aaggatttgg tcagtcaaac
     6061 agttttttat cagcagatta ttggtttaga aatcctatct caaacggata cagaggtcgt
     6121 tctgggactt ggaggaaaag ccttggtaca cttgattcaa gcacaagagg gtggagaagt
     6181 aagggaacat tatggtcttt accatctggc tattcttttg ccgacacgaa aggctttggc
     6241 ggatgtcttg aagcacctga cggatttaca gattcctctt gttggcggtg cagatcacgg
     6301 ttacagtgag gccctttact tagaggactt ggagggaaat ggcattgaac tctatcgaga
     6361 taagccagtt tccacatggg atattcgaga agatggacgt attatcgggg tgactgaagt
     6421 ccttgcggct caggatatct atgagttggg ggaaagagta gagcctttta tcctagcaga
     6481 gggtacgaga atggggcata ttcatctttc tgtcaaggat agtcgaaagt ccagacagtt
     6541 ttatcaaacg gtgttagggc tcgaggataa attcagtgtg cctagtgcta gttggatcgc
     6601 agctggggac taccatcatc atttagcagt caacgaatgg ggaggaaaag gtctggatcc
     6661 gcgtaaacaa gtcctaccag gtttagccta ctatgtcatc gaagtcgcac ataaagaaga
     6721 actgttaacg attgcccaac gagcacaaga agttgacgca ccaatcaaat ggatgacatc
     6781 gatccaattg gaaatcacag actcagatgg catcgtgacc cgtattcgtt tagctagata
     6841 gatggtatgt gatgaaggta gagcatcaat tgtagaaagt gctccttatt ctagtcagat
     6901 acgtggaaaa tttgatatga tagtagggaa taaggagaag aataatatga ctagcgaata
     6961 ccagaaaatg atagcaggag agttttaccg tccatcagac ccagaactga gagccttagc
     7021 tcaggcttct cgccaaaaac aggccgcctt taacaaggaa gagaacccct tgaaggaagc
     7081 cgaaatcatc aagacttggt ttgcctcaac cgggaaaaat ctttacatca acactcgctt
     7141 gatggtggac tacggtgtca acatccatct aggggaaaat ttttattcta attggaactt
     7201 gaccatgctg gatatctgtc ccattcgtat cggggacaat gctatgattg gtcctaattg
     7261 tcagtttttg acacccctcc atccactaga tccacaggaa cgcaattcag gtatcgagta
     7321 cggaaagcct atcacaatcg gagataattt ctggactggt ggtggcgtca ttgtccttcc
     7381 tggagtgaca ctgggaaata atgtcgttgc aggagcaggg gcagtaatta ccaaatcttt
     7441 tggcgacaac gttgtcctag ctggcaatcc tgcgcgcgtg attaaggaaa tacctgttaa
     7501 atagaagtaa aaaggaacag ctggggttgt ttcttttttg taggtttcat cattttttac
     7561 ccagttcaca tttacctact ctatctctta gcaagtctgt ttcattaagc aagttcaaag
     7621 catctcgtaa gtgggatgtt tttctcctca gttcatcagc ttcctccttg acactcggtc
     7681 agattttgat acaatagtac aaaattagag gaggcaggct atgattcaga aacatgcgat
     7741 tcctatttta gagtttgatg acaatcctca ggcggttatc atgcccaatc acgaggggct
     7801 ggacttgcag ttgccaaaga agtgtgttta tgcattttta ggtgaggaga ttgaccgcta
     7861 tgcgagggaa gtaggggcga actgtgttgg cgaatttgtt tctgccacca agacctatcc
     7921 agtttatgtc gtgaactaca aggacgagga gatttgtctg gttcaggctc ctgtcggttc
     7981 agctccagca gcccagttta tggattggtt gattggctat ggtgtggagc agattatctc
     8041 tactgggacc tgtggtgtcc tagctgatat agaggaaaat gcctttctag tccctgttcg
     8101 cgctctgcga gatgaaggag ccagttacca ctatgtggca ccttctcgtt atatggaaat
     8161 gcagccagag gctattgctg ccattgagcg agttttggat gatagaggga ttccttatga
     8221 agaagtcatg acctggtcca cagatggttt ttaccgagaa acggctgaaa aggtggctta
     8281 tcgcaaggaa gaagactgtg ctgttgtgga gatggagtgt tccgctcttg cggcagtagc
     8341 tcaattgcgt ggggttctct ggggtgaatt gttgttcaca gcagattctc tagcggactt
     8401 ggaccagtac gacagtcgtg actggggctc ggaagctttt aataaggcgc tagaactgag
     8461 tttagcaagt gttcaccacc tttagttgta ctggtaaagg atttgtttta tcataaaatg
     8521 tctagctcat acttttcaaa aatatgttta aacgaggtca ccttcctctt gtcctaggca
     8581 tgttgaggtt gggaaaaatc tttaaaatca gaaaaacgta tcatatcagg tgatgaaaac
     8641 tttgacacta tgcgttttat gtcgataaga tttagagtga gatgaaatga tactcttcga
     8701 aaatctcttc aaaccaggtc agcttcacct tgccgtaggt atatgttact gacttcgtca
     8761 gtcttatccg gcaacctcaa aacggtgttt tgagctgact tcgtcagttc tatttgcaac
     8821 ctcaaaacag tgttttgagc aacctgtgac tagctttcta atcgatgcct tggttttcat
     8881 tgcctataat caaaaagaga aattttctcc tgaaaagcat atagagtagc tggcgttaaa
     8941 agctcctgtc ttgctttttt gacctatagt cacatctatc aagtattgtt cttgcctaag
     9001 ctatcaataa aaaggtggca ttttttaggc ttggtgttag tagattttgc cttatcctat
     9061 ctaagtcatt tcgaactttt tatggtacaa tggaaacatg ttattcaaat tatctaagga
     9121 aaaaatagag ctaggcttat ctcgtttatc gccagcccgt cgtatttttt tgagttttgc
     9181 cttggtcatt ttactaggct ctcttctttt gagcttgccc tttgtccaag ttgaaagctc
     9241 acgagcgact tattttgatc atcttttcac tgctgtatct gcagtctgtg tgacgggtct
     9301 ctcaaccctt ccagtagctc acacctataa tatctggggt caaataatct gtttgctctt
     9361 gattcagatc ggtggtctag ggctcatgac ctttattggg gttttctata tccagagcaa
     9421 gcaaaagctt agtcttcgta gccgtgcaac tattcaggat agttttagtt atggagaaac
     9481 tcgatctttg agaaagtttg tctattctat ttttctcacg acctttttgg ttgagagctt
     9541 gggagctatt ttgcttagtt ttcgccttat tcctcaactt ggctggggac gtggtctttt
     9601 tagttccatt tttctagcga tctcagcctt ctgtaatgcc ggttttgata atttagggag
     9661 caccagttta tttgcttttc agaccgattt actggtcaat ctggtgattg caggcttaat
     9721 tattacaggc ggccttggtt ttatggtctg gtttgatttg gctggtcatg taggaagaaa
     9781 gaaaaaagga cgtctgcact ttcatacgaa gcttgtacta ttgttgacta taggtttgtt
     9841 gttatttgga acggcaacta ctctctttct tgagtggaac aatgctggaa cgattggcaa
     9901 tctccctgtt gccgataagg ttttagttag cttttttcaa acagtgacga tgcgaacagc
     9961 tggcttttct acgatagatt atactcaggc tcatcctgtg actcttttga tttatatctt
    10021 acagatgttt ctaggtgggg cacctggagg aacagctggg ggactcaaga ttacgacatt
    10081 ttttgtcctc ttggtctttg cacgaagtga gcttctaggc ttgcctcatg ccaatgttgc
    10141 gagacgaacg attgcgccgc gaacggttca aaaatccttt agtgtcttta ttatcttttt
    10201 gatgagcttc ttaataggat tgattctgct agggataaca gccaaaggca atcctccctt
    10261 tatccacctc atatttgaaa ccatttcagc tcttagtaca gttggtgtaa cggcaaatct
    10321 gactcctgac cttgggaaat tggctctcag tgttatcatg ccacttatgt ttatgggacg
    10381 aattggtccc ttgaccttgt ttgttagctt ggcagattac catccagaaa agaaagatat
    10441 gattcactat atgaaagcag atattagtat tggttaagaa aggaaagagc atgtcagatc
    10501 gtacgattgg aattttgggc ttgggaattt ttgggagcag tgtcctagct gccctagcca
    10561 agcaggatat gaatattatc gctattgatg accacgcaga gcgcatcaat cagtttgagc
    10621 cagttttggc gcgtggagtg attggtgaca tcacagatga agaattattg agatcagcag
    10681 ggattgatac ctgcgatacc gttgtagtcg cgacaggtga aaatctggag tcgagtgtgc
    10741 ttgcggttat gcactgtaag agtttggggg taccgactgt tattgctaag gtcaaaagtc
    10801 agaccgctaa gaaagtgcta gaaaagattg gagctgactc ggttatctcg ccagagtatg
    10861 aaatggggca gtctctagca cagaccattc ttttccataa tagtgttgat gtctttcagt
    10921 tggataaaaa tgtgtctatc gtggagatga aaattcctca gtcttgggca ggtcaaagtc
    10981 tgagtaaatt agacctccgt ggcaaataca atctgaatat tttgggtttc cgagagcagg
    11041 aaaattcccc attggatgtt gaatttggac cagatgacct cttgaaagca gatacctata
    11101 ttttggcagt catcaacaac cagtatttgg ataccctagt agcattgaat tcgtaaagag
    11161 ggatgacccc tcttttttga tgcccaagat ggcaaataga gacagaagcc ccttgtcttc
    11221 tagtaaaagt tcttcaaagg ctggacttta tggtaaaata gaaggaagtg acaagagaga
    11281 gtaatactca atgaaaatca aagatcaaac taggaaacta gctacgggct gcttaaaaca
    11341 ctgttttgag gttgtggata gaactgacga agtcagctca aaacactgtt ttgaggttgt
    11401 ggatagaact gacgaagtca gctcaaaaca ctgttttgag gttgcagata gaactgacga
    11461 agtcagtaac atctatacgg caaggcgatg ttgacgcggt ttgaagagat tttcgaagag
    11521 tataagaaaa aatcagtccc ctaaaggagc agattatgaa gttattgtct atcgccattt
    11581 ctagctataa tgcagcagcc tatcttcatt actgtgtgga gtcgctagtg attggctggg
    11641 accaggtcgg aaatttctcc aaaggccagt atatcatgat gcactcgctg atttatcgga
    11701 cagatttgtt gcgtgctagc cagttctaat tgcctgaaca tactttttat gtcgataatc
    11761 tctttgtctt tacgcccctt cagcaggtca agaccatgta ctatctgcct gtcgatttct
    11821 atcgttattt gattgggcgt gagggccagt ctgtcaatga gcaagtgatg attaagtgca
    11881 ttgaccagca actcaaggtc aatcgactct tggtagacca gcttgatttg tcccaagtga
    11941 gtcatcccaa aatgcgagaa tatctgctga atcatattga aatcacgacg gtgatttcca
    12001 gtaccctgct caaccgatct gaaacagcgg agcatctggc aaaaaaacgc caattgtgga
    12061 cctatattca gcagaaaaat ccagaagtct ttcaggctat tcgtaagacc atgttgagcc
    12121 gtttgaccaa acattctgtc ttgccagatc gcaaactgtc caatgtcgtc tatcaaatca
    12181 ccaaatctgt ttatggattt aattaatgta agtgttttat aagagggatt taagaaaaat
    12241 tttaactttt tcttagtcct ttttaatttc aggagattat actagagtca tcaaataaag
    12301 aaagactcta aggagaatcc tatgaaattc aatccaaatc aaagatatac tcgttggtct
    12361 attcgccgtc tcagtgtcgg tgttgcctca gttgttgtgg ctagtggctt ctttgtccta
    12421 gttggtcagc caagttctgt acgtgccgat gtggtcaatc cgaccccagg tcaagtctta
    12481 cctgaagaga catcgggaac gaaagagggt gacttatcag aaaaaccagg agacaccgtt
    12541 ctcactcaag cgaaacctga gggcgttact ggaaatacga attcacttcc gacacctaca
    12601 gaaagaactg aagtgagcga ggaaacaaac tcttctagtc tggatacact ttttgaaaaa
    12661 gatgaagaag ctcaaaaaaa tccagagcta acagatgtct taaaagaaac tgtagataca
    12721 gctgatgtgg atgggacaca agcaagtcca gcagaaacta ctcctgaaca agtaaaaggt
    12781 ggagtgaaag aaaatacaaa agacagcatc gatgttcctg ctgcttatct tgaaaaagct
    12841 gaagggaaag gtcctttcac tgccggtgta aaccaagtaa tcccttatga actattcgct
    12901 ggtgatggta tgttaactcg tctattacta aaagcttcgg ataatgctcc ttggtctgac
    12961 aatggtactg ctaaaaatcc tgctttacct cctcttgaag gattaacaaa agggaaatac
    13021 ttctatgaag tagacttaaa tggcaatact gttggtaaac aaggtcaagc tttaattgat
    13081 caacttcgcg ctaatggtac tcaaacttat aaagctactg ttaaagttta cggaaataaa
    13141 gacggtaaag ctgacttgac taatctagtt gctactaaaa atgtagacat caacatcaat
    13201 ggattagttg ctaaagaaac tgtcgaaaaa gctgtta
//
LOCUS       DAVKJJ010000048        13217 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000048 DAVKJJ010000000
VERSION     DAVKJJ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13217
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.048"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            73..1773
                     /gene="dnaG"
                     /locus_tag="VW144_001603"
                     /pseudo
     CDS             73..1773
                     /gene="dnaG"
                     /locus_tag="VW144_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014178.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
     gene            1851..2960
                     /gene="rpoD"
                     /locus_tag="VW144_001604"
     CDS             1851..2960
                     /gene="rpoD"
                     /locus_tag="VW144_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU9601022.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            2974..3303
                     /locus_tag="VW144_001605"
     CDS             2974..3303
                     /locus_tag="VW144_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9601023.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            3418..4461
                     /gene="cpoA"
                     /locus_tag="VW144_001606"
     CDS             3418..4461
                     /gene="cpoA"
                     /locus_tag="VW144_001606"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEU9601024.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            4477..5802
                     /locus_tag="VW144_001607"
     CDS             4477..5802
                     /locus_tag="VW144_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU9601025.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRSSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            5833..6009
                     /locus_tag="VW144_001608"
     CDS             5833..6009
                     /locus_tag="VW144_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9601026.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            6068..6202
                     /locus_tag="VW144_001609"
     CDS             6068..6202
                     /locus_tag="VW144_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEU9601027.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            6266..7576
                     /gene="obgE"
                     /locus_tag="VW144_001610"
     CDS             6266..7576
                     /gene="obgE"
                     /locus_tag="VW144_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU9601028.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEDFKKKLAENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            7586..7744
                     /locus_tag="VW144_001611"
     CDS             7586..7744
                     /locus_tag="VW144_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601029.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            8887..10146
                     /locus_tag="VW144_001612"
     CDS             8887..10146
                     /locus_tag="VW144_001612"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9601030.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            10162..10713
                     /locus_tag="VW144_001613"
     CDS             10162..10713
                     /locus_tag="VW144_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9601031.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENKASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            10706..11983
                     /locus_tag="VW144_001614"
     CDS             10706..11983
                     /locus_tag="VW144_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9601032.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            11999..12859
                     /locus_tag="VW144_001615"
     CDS             11999..12859
                     /locus_tag="VW144_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEU9601033.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVAKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
BASE COUNT         4009 a         2266 c         3069 g         3873 t
ORIGIN      
        1 tagggaaaat gagtgaaatg gtttactttt tttctgaaat aaagtatact atataaagta
       61 aactatgata acatggaggt attgtgtatg gttgacaaac aagtcattga agaaatcaaa
      121 aacaatgcca acattgtgga agtcatagga gatgtgattt ctttacaaaa ggcaggacgg
      181 aactatctag ggctctgtcc ttttcatggt gaaaaaacac cttctttcaa cgttgtagag
      241 gacaagcagt tttaccactg ttttggttgt ggtcgctcag gtgatgtctt taaattcatc
      301 gaggagtacc aaggggttac ctttatggag gctgtccaaa tcttaggtca gcgtgtcggg
      361 attgaggttg aaaaaccgct ttatagtgaa cagaagccag cctcgcctca ccaagctctt
      421 tatgatatgc acgaagatgc agccaaattt taccatgcta ttctcatgac aacgactatg
      481 ggagaagagg ccagaaatta cctttatcag cggggtttga cagatgaagt gcttaaacat
      541 ttttggattg gtttagcacc tccagaacga aactatctct atcaacgttt gtctgatcag
      601 tatcgtgaag aggatttact ggattcaggc ctgttttatc tctcggatgc caatcaattt
      661 gtagacacct ttcacaatcg cattatgttt cccctgacaa atgaccaagg aaaggtcatt
      721 gccttctcag gtcgtatctg gcaaaaaacg gattcacaaa cttctaagta taaaaacagc
      781 cgatcgactg caatttttaa caaaagttac gaattatatc atatggatag ggcaaaaaga
      841 tcttctggaa aagctagtga gatttacctg atggaaggat tcatggatgt tattgcagcc
      901 tatcgggctg gaatcgaaaa tgctgtggcg tcgatgggaa cggccttgag tcgagagcat
      961 gttgagcatc tgaaaaggtt aaccaagaaa ttggttcttg tttacgatgg agataaggct
     1021 gggcaagccg cgatattgaa agcattggat gaaattggtg atatgcctgt gcaaatcgtt
     1081 agcatgcctg ataacttgga tcctgatgag tatctacaaa aaaatggtcc agaagacttg
     1141 gcctatctat taacaaaaac tcgtattagt ccgattgagt tctacattca tcagtacaaa
     1201 cctgaaaaca gtgaaaatct gcaggctcag attgagttta ttgaaaaaat agctcccttg
     1261 attgttcaag aaaagtccat cgctgctcaa aacagctata ttcatatttt agctgacagt
     1321 ctggcgtcct ttgattatac ccagattgag cagattgtta atgagagtcg tcaggtgcaa
     1381 aggcagaatc gcatggaagg aatttccaga ccgacgccaa tcaccatgcc tatcaccaag
     1441 cagttatcgg ctattatgag ggcagaagcc catctactct atcggatgat ggaatcccct
     1501 cttgttttga acgattaccg tttgcgagaa gactttgcat ttgctacacc tgaatttcag
     1561 gtcttatatg acttgcttgg ccagtatgga aatcttcctc cagaagtttt agcagagcag
     1621 acagaggaag ttgaaagagc ttggtaccaa gttttagctc aggatttgcc tgctgagata
     1681 tcgccgcagg aacttagtga agtagagatg actcgaaaca aggctctctt gaatcaggac
     1741 aatatgagaa tcaaaaagaa ggtgcaggaa gctagccatg taggagatac agatacagcc
     1801 ctagaagaat tggaacgttt aatttcccaa aagagaagaa tggagtaata atggcaacaa
     1861 aacaaaaaga agtaacaaca tttgacgtac aggtagcaga atttatccgt aatcataagc
     1921 aaaaagggac agcaacagat gatgaaatca atgcaagtct ggttattcct tttaccttgg
     1981 acgctgatgg gattgaagat ctcttgcaac ggattcagga tgcaggcatt tctatcacag
     2041 ataacgaagg aaatccaagt gcgcgtgttc ttagcaatga agaagaacca gaactcagcg
     2101 atgaggactt gattgggtca acttctgcta aggtcaatga ccctgtccgt atgtacttga
     2161 aagaaatagg ggtcgttcct ctcttgacca atgaagagga gaaagagttg gcactggctg
     2221 ttgaagctgg cgatatcgaa gccaaacaac gtcttgcgga agccaatctt cgtttggttg
     2281 tttctattgc caaacgctat gtcggtcgtg gcatgcagtt ccttgacttg attcaagaag
     2341 gaaatatggg cttgatgaag gcggttgaca agtttgacta ttctaaaggg ttcaagtttt
     2401 caacttatgc aacttggtgg attcgtcagg ctatcactcg tgctattgcg gatcaagctc
     2461 gtaccatccg tatcccagtt cacatggttg aaactatcaa taaattggtt cgtgaacagc
     2521 ggaatctcct tcaagaattg gggcaagatc cgacaccaga acagattgct gaacgaatgg
     2581 atatgacacc tgataaggtt cgtgaaatct tgaagattgc ccaagaacca gtatctcttg
     2641 aaactcctat cggtgaagag gacgatagcc accttggaga ctttatcgaa gatgaagtaa
     2701 ttgaaaatcc agtggattat acgactcgta tcgtcttgcg tgagcaattg gatgaaatct
     2761 tagatactct tacagaccgt gaagaaaatg ttctgcgtct acgttttgga ctagatgatg
     2821 gaaaaatgcg cacacttgaa gatgtgggga aagtctttaa cgtaactcgt gagcgtatcc
     2881 gtcagattga agcaaaggct ttgagaaaac tacgccaacc aagccgcagc aaaccgcttc
     2941 gtgattttat tgaagactaa gagtgaggaa aatatggctt atacagaaga gcaaattgaa
     3001 aacatcaaaa cacggatttt aacagccttg gaagaagtca tcgaccctga gttgggaatc
     3061 gatattgtca atcttggttt gatctatgag attcgttttg acggtgacac agggcaaaca
     3121 gagattgaca tgactttgac aactatgggt tgtcccttag cagacctttt gacagaccag
     3181 atttatgatg ctatgataga ggttccagaa gtaacggata ctgaggttaa attagtctgg
     3241 tatccagcct ggacagttga aaaaatgagt cgctatgccc gcattgccct aggcattaag
     3301 taaacatata aaagcatgtg agagactgtt ggaaaagcga ggaaatttcc cctcttttcc
     3361 tctagtctct cctttctttt gctgatttta ttcaaagaaa atgatataat agtagttatg
     3421 gagaaaaaga aattacgcat caatatgttg agttcaagtg agaaagtagc aggacaggga
     3481 gtttcaggtg cttaccgtga attagttcgt cttcttcacc gtgctgccaa ggaccaattg
     3541 attgttacag aaaatcttcc aatcgaggca gatgtgactc actttcatac gattgatttt
     3601 ccctattatt tatcaacctt ccaaaagaaa cgctcaggga gaaagattgg ctatgtgcat
     3661 ttcttgccag ctacacttga aggaagtttg aaaattccat ttttcttaaa gggaattgtg
     3721 aaacgctatg tattttcttt ttacaaccgg atggagcact tggttgtggt caatcctatg
     3781 tttattgagg atttggtagc agctggtatt ccacgtgaaa aagtgaccta tattcctaac
     3841 tttgtcaaca aggaaaaatg gcatcctcta ccacaagaag aggtagtcag actgcgcaca
     3901 gatcttggtc ttagtgacaa tcagtttatc gtagtaggtg ctgggcaagt tcagaaacgt
     3961 aaagggattg atgactttat ccgtctggct gaggaattgc ctcagattac ctttatctgg
     4021 gctggtggct tctcttttgg tggtatgaca gatggttatg aacactataa gaaaattatg
     4081 gaaaatcccc ctaaaaattt gatttttcca ggcattgtat cgccagagcg gatgcgcgaa
     4141 ttgtatgctc tagcggatct tttcttgttg cctagttaca atgagctctt tcctatgact
     4201 attttagaag ctgcgagttg tgaggctcct attatgttgc gtgatttaga tctctataag
     4261 gtgattttgg agggaaatta tcgggcgaca gcgggtagag aagagatgaa agaggctatt
     4321 ttggaatatc aagcaaatcc tgctgtctta aaagatctca aagaaaaggc caagaatatt
     4381 tccagagagt attctgaaga gcatctgtta caaatctggt tggactttta tgagaaacaa
     4441 gccgctttag ggagaaagta aaaagtgagg taatctatgc gaattggttt atttacagat
     4501 acctattttc ctcaggtttc tggtgttgcg accagtattc gaaccttgaa aacagaactt
     4561 gaaaagcagg gacatgctgt ttttatcttt acgacgacag ataaggatgt caatcgctac
     4621 gaagattggc aaattatccg cattccaagt gttcctttct ttgcttttaa ggatcgtcgc
     4681 tttgcctacc gaggttttag caaagcactt gaaattgcta aacagtatca gctagatatt
     4741 atccatactc agacagaatt ttctcttggc ctgttgggga tttggattgc gcgtgaattg
     4801 aaaattccag tcatccatac ctatcacacc cagtatgaag actatgtcca ttatattgct
     4861 aaggggatgt tgatccggtc gagtatggtc aagtatctgg ttagaggttt cctgcatgat
     4921 gtggatgggg ttatttgccc tagtgagatt gtccgtgact tgctatctga ttataaggtc
     4981 aaggttgaaa aacgggtcat tcctactggg attgaattag ccaagtttga gcgtccggaa
     5041 atcaagcagg aaaatttgaa agaactgcgt agtaaactag ggattcaaga tggtgaaaag
     5101 acgttgctta gtctttcgag aatctcctat gaaaaaaata ttcaagcagt tttagcagcc
     5161 tttgctgatg ttctgaaaga ggaagacaag gttaaactgg tagtagctgg ggatggccct
     5221 tatctgaatg acctcaaaga gcaagcccag aacctagaga ttcaagactc agtcatcttt
     5281 acagggatga ttgctcctag tgagacggct ctttactata aagcggcgga tttcttcatt
     5341 tcggcatcga caagcgaaac gcaaggtttg acctacttgg aaagcttagc cagtggaaca
     5401 cctgtcattg ctcacggaaa tccttatttg aacaacctca tcagtgataa aatgtttgga
     5461 accttgtact atggagaaca tgatttggct ggtgctattt tggaagccct gattgcaaca
     5521 ccagacatga acgagcatac cttatcagag aaattgtatg agatttcagc tgagaacttt
     5581 gggaaacgag tgcatgagtt ttatctggat gccattattt caaataactt ccagaaagat
     5641 ttggctaaag atgatacggt cagtcagcgt atctttaaga cagttttgta tcttcagcaa
     5701 caggtggttg ctgtacctgt aaaaggatct agacgcatgt tgaaggcttc aaaaacacag
     5761 ttgatcagta tgagagacta ttggaaagac catgaagaat agaaagagga acagctatga
     5821 aaaaaacaat taatgagaag cggtcgtgat aaaaagattg cgggtgtttg tgctggggtg
     5881 gcccattatc tggatatgga tccgactatc gttcaagtca tttggggtgt tcttacttgc
     5941 tgttacggag ctggaattgt agcttacatt attttatgga ttatcgcacc agtagcaact
     6001 gactattgaa aactagctca attcatgcta agataataga aaatagaatg taacgaagga
     6061 gagaaaaatg gcatttggag ataatggaaa tcgtaaaaaa actatgtttg agaaaataac
     6121 cttgtttatc gtgattatca tgctagtagc aagtttattg ggaatttttg caactgcaat
     6181 tggtgccctc agtaatctat aaaatagatt caagaaaatt tagtgactgg gatttcccag
     6241 ccctttttta aagtgagaag aaataatgag tatgttttta gatacagcta agattaaggt
     6301 caaggctggt aatggtggcg atggtatggt tgcctttcgt cgtgaaaaat atgtccctaa
     6361 tggaggccct tggggtggtg atggtggtcg tggaggcaat gtggtcttcg ttgtagacga
     6421 aggactacgt accttgatgg atttccgcta caatcgtcat ttcaaggctg attctggtga
     6481 aaaagggatg accaaaggga tgcatggtcg tggtgctgag gaccttagag ttcgagtacc
     6541 acaaggtacg actgttcgtg atgcggagac tggcaaggtt ttaacagatt tgattgaaca
     6601 tgggcaagaa tttatcgttg cccacggtgg tcgtggtgga cgtggaaata ttcgtttcgc
     6661 gacaccaaaa aatcctgcac cggaaatctc tgaaaatgga gaaccaggtc aggaacgtga
     6721 gttacaattg gaactaaaaa tcttggcaga tgtcggttta gtaggattcc catctgtagg
     6781 gaagtcaaca cttttaagtg ttattacctc agctaagcct aaaattggtg cctaccactt
     6841 taccactatt gtaccaaatt taggtatggt tcgcacccaa tcaggtgaat cctttgcagt
     6901 agccgacttg ccaggtttga ttgaaggggc tagtcaaggt gttggtttgg gaactcagtt
     6961 cctccgtcac atcgagcgta cacgtgttat ccttcacatc attgatatgt cagctagcga
     7021 aggccgtgat ccatatgagg actacctagc tatcaataaa gagctggagt cttacaatct
     7081 tcgcctcatg gagcgtccac agattattgt agctaataag atggacatgc ctgagagtca
     7141 ggaaaatctt gaagacttta agaaaaaatt ggctgaaaat tatgatgaat ttgaagagtt
     7201 accagctatc ttcccaattt ctggattgac caagcaaggt ctggcaacac ttttagatgc
     7261 tacagctgaa ttgttagaca agacaccaga atttttgctc tacgacgagt ccgatatgga
     7321 agaagaagct tactatggat ttgacgaaga agaaaaagcc tttgaaatta gtcgtgatga
     7381 cgatgcgaca tgggtacttt ctggtgaaaa actcatgaaa ctctttaata tgaccaactt
     7441 tgatcgtgat gaatctgtca tgaaatttgc ccgtcagctt cgtggtatgg gggttgatga
     7501 agcccttcgt gcgcgtggag ctaaagatgg ggatttggtc cgcattggta aatttgagtt
     7561 tgaatttgta gactaggaga ctggtatggg agataaaccg atatctttcc gagatgcgga
     7621 tggtaatttt gtttccgccg cagacgtttg gaatgaaaag aaattggaag aactatttaa
     7681 tcgtctcaat ccaaatcgtg ccttgagatt ggcacgaact aaaaaggaaa atccatctca
     7741 gtaaagaagc taaaaaatcc cgtgcctcat cagacacggg attttgtggt acgacaggca
     7801 tgtatagcaa actgaatctg gaatagcaca gcatatcttc taaaatatag taaaatgaaa
     7861 tgagaacagg acaaatcgat caggacagta aaatcgattt ctaacaatgt tttagaagca
     7921 gagatgtact attctagttt caatcaacta tattgttata aattgatttg aatttcaaaa
     7981 ttaaattgtt tgattcttat ttcaatttgt tatagtatat ctgatgtcaa agttctcggc
     8041 gagtcaaata gcgattccca agcctgacta tcgtgaggta gcagattaaa atggtctggg
     8101 gatagaccgt tttaagtctg acgctggaaa taagaattgt cagaagaagg gatagcgaaa
     8161 tcgtggctct acgaacagga acgtgataat aaggcgtata tagcggataa gggggtctca
     8221 aactctaaag tccaaaaagg tagtcgtaac ctatatgcgt aaatcacgag agtaattgaa
     8281 ttcgtactaa gattttctat tttcactgta accttttaac gtccttatat cttgtataca
     8341 cgaggaaaga tgtacgactt atcccgtgag gtctatcact ataaagagaa aacgacagat
     8401 agaagtgatc ctgagtcacg gttatctgtc tgataggacg gtatgtataa aacgcttctg
     8461 tgaactgaga gaagggggag aagttcttgc taaaatttag ttgaacagcc gtattccgat
     8521 acttagataa gagatctagt cttagctcct actcagtttt aggggataaa aaaggggcaa
     8581 tagcgattcg agaaagatta tactcttcga aaatctcttc aaatcacgtc aatatcgcct
     8641 tgtcgtatgt gtaggatact gactacgtca gttccatcta caacctcaaa acagtgtttt
     8701 gagcaacctg cggctagttt cctagtttga tctttgattt tcattgagta ttagtaattc
     8761 agttactaac tcgtcaactc tgatttatcc aataaaattg aaaaggatgg aaaaaaggat
     8821 aaatttatga tatactttat tttgaagacc ttattagaaa tcttgaaaga gtattgaaaa
     8881 cttagaatga gaaaaattgt tatcaatggt ggattaccac tgcaaggtga aatcactatt
     8941 agtggtgcta aaaatagtgt cgttgcctta attccagcta ttatcttggc tgatgatgtg
     9001 gtgactttgg attgcgttcc agatatttcg gatgtagcca gtcttgtcga aatcatggaa
     9061 ttgatgggag ctactgttaa gcgttatgac gatgtattgg agattgaccc aagaggtgtt
     9121 caaaatattc caatgcctta tggtaaaatt aacagtcttc gtgcatctta ctatttttat
     9181 gggagcctct taggccgttt tggtgaagcg acagttggtc taccgggagg atgtgatctt
     9241 ggtcctcgtc cgattgactt acaccttaag gcgtttgaag ctatgggtgc cactgctagc
     9301 tacgagggag ataacatgaa gttatctgct aaagatacag gacttcatgg tgcaagtatt
     9361 tacatggata cggttagtgt gggagcaacg attaatacga tgattgctgc ggttaaagca
     9421 aatggtcgta ctattattga aaatgcagcc cgtgaacctg agattattga tgtagctact
     9481 ctcttgaata atatgggtgc ccatatccgt ggggcaggaa ctaatatcat cattattgat
     9541 ggtgttgaaa gattacatgg gacacgtcat caggtgattc cagaccgcat tgaagctgga
     9601 acatatatat ctttagctgc tgcagttggt aaaggaattc gtataaataa tgttctttac
     9661 gaacacctgg aagggtttat tgctaagttg gaagaaatgg gagtgagaat gactgtatct
     9721 gaagacagca tttttgtcga ggaacagtct aatttgaaag caatcaatat taagacagct
     9781 ccttacccag gctttgcaac tgatttgcaa caaccgctta cccctctttt actaagagcg
     9841 aatggtcgtg gtacaattgt cgatacgatt tacgaaaaac gtgtaaatca tgtttttgaa
     9901 ctagcaaaga tggatgcgga tatttcgaca acaaatggtc atattttgta cacgggtgga
     9961 cgtgatttac gtggggccag tgttaaagcg accgacttaa gagctggggc tgcactagtc
    10021 attgctgggc ttatggctga aggtaaaact gaaattacca atatcgagtt tatcttacgt
    10081 ggttattctg atattatcga aaaattacgt aatttaggag cggatattag acttgttgag
    10141 gattaaaccg tagaggtgtt tatgaatatt tggaccaaat tagcaatgtt ttcttttttt
    10201 gaaacggatc gcttgtattt gcgtcctttc ttttttagtg atagtcagga cttccgcgag
    10261 atagcttcaa atccagaaaa tcttcaattt attttcccaa cgcaggcaag tctggaagaa
    10321 agtcaatatg cactggccaa ttactttatg aagtcccctt tgggagtgtg ggcaatttgt
    10381 gaccagaaaa atcaacaaat gattggttct attaaatttg agaagttaga tgaaatcaaa
    10441 aaagaagctg agcttggcta ttttttgaga aaagatgctt ggtcgcaagg atttatgaca
    10501 gaggttgtta gaaaaatttg tcagctttct tttgaggaat ttggcttaaa acaattattt
    10561 atcattaccc accttgaaaa taaagctagc caaagagttg ctcttaagtc tggatttagt
    10621 ttgttccgtc agtttaaggg aagtgatcgt tacacaagaa aaatgcggga ttatcttgaa
    10681 tttcggtatg taaaaggaga gttcaatgag taagcatcag gaaattctaa gctatttgga
    10741 ggaattacca gtaggtaaaa gggtcagtgt tcgtagcatt tcgaatcatc taggagttag
    10801 tgatggaaca gcctatcggg ctattaaaga agctgaaaac cgtggaattg tggagacccg
    10861 tcctagaagt ggaacaattc gtgttaaatc ccagaaagtt gctatagaga gattaacgtt
    10921 tgctgaaatt gcagaagtga cttcttctga ggttctggct gggcaagaag gtttagagag
    10981 agagtttagt aagttttcaa ttggtgccat gactgaacaa aatatcttgt cttaccttca
    11041 tgatgggggg ctcttgattg tcggagaccg aacccgtatt cagttgctag ccttggaaaa
    11101 tgaaaatgca gttctggtta cagggggatt tcaggttcat gatgatgtgc ttaaactggc
    11161 caatcaaaaa gggattcctg ttctaagaag taagcatgat acctttaccg tcgcgaccat
    11221 gatcaataaa gccttgtcaa atgtccaaat caagactgat attctgacag ttgagaaact
    11281 ttatcgccct agtcatgagt atggttttct aagagagaca gatacagtta aagattattt
    11341 ggacttggtt cgtaagaatc gtagcagccg tttccctgtt atcaatcaac atcaggtcgt
    11401 tgttggtgtt gtaaccatga gagacgctgg tgataaatca ccaagcacga caattgataa
    11461 ggttatgtct cgtagtctat ttttggttgg attatcgaca aatattgcca atgtgagtca
    11521 acggatgatc gcagaagact ttgaaatggt accagttgtt cgaagcaatc aaactttgct
    11581 tggcgttgtg acgcgacgag atgtcatgga gaagatgagc cgttcccaag tttcggctct
    11641 accaactttt tctgagcaga ttggacaaaa gctctcttat caccatgatg aagtagtcat
    11701 tacagtggaa ccctttatgc tagaaaaaaa tggagttttg gctaatggtg tattggcaga
    11761 aattctgacc cacatgaccc aagatttagt tgttaatagt ggtcgcaatc tcattatcga
    11821 gcagatgctg atctactttt tgcaggctgt tcagatagat gatatattgc gcattcaggc
    11881 acggattatt catcatacga gacggtcagc tataattgat tacgatattt atcatggtca
    11941 ccagattgtt tcaaaagcaa atgtgactgt taaaattaat tagaaactag gagaaaagat
    12001 gataacatta aaatcagctc gtgaaatcga agctatggac aaggctggtg attttctagc
    12061 aagtattcat ataggcttac gtgatttgat taagccaggc gtagatatgt gggaagttga
    12121 agaatatgtc cgccgtcgtt gtaaagaaga aaatttcctt ccacttcaga ttggggttga
    12181 cggtgccatg atggactatc cttatgctac ctgttgctct cttaacgatg aagtggctca
    12241 cgctttccct cgtcattata tcttgaaaga tggtgatttg ctcaaagttg atatggtttt
    12301 gggaggtccc attgctaaat ctgacctaaa tgtctcaaaa ttaaacttca acaatgttga
    12361 acaaatgaaa aaatacactc agagctattc tggtggttta gcagactcat gttgggctta
    12421 tgctgttggt acaccgtccg aagaagtcaa aaacttgatg gatgtagcca aagaagctat
    12481 gtacaagggt attgagcaag ctgttgttgg aaatcgtatc ggtgatatcg gtgcggctat
    12541 tcaagaatac gctgaaagtc gtggttacgg tgtagtgcgt gatttggttg gtcatggtgt
    12601 tggcccaact atgcacgaag aaccaatggt tcctaactat ggtattgcag gtcgtggact
    12661 ccgtcttcgt gaaggaatgg tcttaaccat tgaaccaatg atcaatacag gcgattggga
    12721 aattgataca gatatgaaaa ctggttgggc gcataagacc attgacggtg gattgtcatg
    12781 tcagtatgaa caccaatttg tcattacgaa agatggacct gttatcttga ctagccaagg
    12841 tgaagaagga acttattaat aaaaagtgaa aagactactg gaagtttatt ttgataaaaa
    12901 atccagtaga tcttttcata ataaaacgca ttgtatcaag tgttaggggc tgatatcatg
    12961 cgtttttctg cttttaagat tttttccaac tctgtttgta agcgcatcat aacaaagggt
    13021 ctaggattca gggctctcct cctatatact attagtaaag taaaactaag agaggatatt
    13081 ttagtgtcgc agtctattgt tcctgtagag attccacaat attgtcgttt tgattctaaa
    13141 aagagaaatg gaattctgtt taacgttcgt attgccaatc ttaaatttac cttttttaga
    13201 ttagacttcc tgcgaaa
//
LOCUS       DAVKJJ010000049        13094 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000049 DAVKJJ010000000
VERSION     DAVKJJ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13094
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.049"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            37..258
                     /locus_tag="VW144_001616"
     CDS             37..258
                     /locus_tag="VW144_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601034.1"
                     /translation="MILMTKNINLTNEELELIQGGADPYGKEPNGYYPWKMEPVLTLL
                     VHGFCPRDTDDLGYIGGGNHLCKGSAARF"
     gene            312..983
                     /locus_tag="VW144_001617"
     CDS             312..983
                     /locus_tag="VW144_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9601035.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRLFNYLAKPYLPASREFFQIL
                     LLMESGVLLLAVIYLLVFAGKKTFHFKWQLRYFIYLLLGYITSYMSDFLFSYFISLSS
                     NQISLNETIEMIGRQELPYFLLIVCFIGPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNIYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(1034..1231)
                     /locus_tag="VW144_001618"
     CDS             complement(1034..1231)
                     /locus_tag="VW144_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9601036.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            1224..2132
                     /locus_tag="VW144_001619"
     CDS             1224..2132
                     /locus_tag="VW144_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9601037.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLARKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            2129..2590
                     /locus_tag="VW144_001620"
     CDS             2129..2590
                     /locus_tag="VW144_001620"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU9601038.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRASQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            2580..3467
                     /locus_tag="VW144_001621"
     CDS             2580..3467
                     /locus_tag="VW144_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9601039.1"
                     /translation="MEIKIETGGLRLDKALSDLTELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            3470..5365
                     /gene="cbpE"
                     /locus_tag="VW144_001622"
                     /gene_synonym="pce"
     CDS             3470..5365
                     /gene="cbpE"
                     /locus_tag="VW144_001622"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEU9601040.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREEIETSYKHVLKDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDNRITNSERLWDNLYGYDKVLQT
                     ASEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSSSPIGSYDSGQEINREYISWLESMGIQYINAASKDYDATVFDIRQDGLVNISTSYK
                     PIPSFQAGWHKSSYGNWWYQAPDSTGEYVVGWNEIKGEWYYFNQTGILLQNQWKKWNN
                     HWFYLTDSGASAKNWKKIDGIWYYFNKENQMEIGWVQDKEQWYYLDVDGSMKTGWLQY
                     MGQWYYFAPSGEMKMGWVKDKETWYYMDSTGVMKTGEIEVAGQHYYLEDSGAMKQGWH
                     KKANDWYFYKTDGSRAVGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVS
                     IEKSATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNFSTSQDLTSSTSQSSETSV
                     NKSESEQ"
     gene            5445..6575
                     /locus_tag="VW144_001623"
     CDS             5445..6575
                     /locus_tag="VW144_001623"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEU9601041.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            6585..7847
                     /locus_tag="VW144_001624"
     CDS             6585..7847
                     /locus_tag="VW144_001624"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEU9601042.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADDDKALSIINNAKTSRPSVCNAMEVLLVHEDKAASFLPRLEQ
                     VLVADRKEAGLEPIQFRLDSKASQFVSGQAAETQDFDTEFLDYVLAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            7851..8648
                     /gene="proC"
                     /locus_tag="VW144_001625"
     CDS             7851..8648
                     /gene="proC"
                     /locus_tag="VW144_001625"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU9601043.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFCQLLSKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLSRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            8868..9506
                     /locus_tag="VW144_001626"
     CDS             8868..9506
                     /locus_tag="VW144_001626"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU9601044.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            9503..10393
                     /locus_tag="VW144_001627"
     CDS             9503..10393
                     /locus_tag="VW144_001627"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU9601045.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEIAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            10433..10750
                     /gene="yabA"
                     /locus_tag="VW144_001628"
     CDS             10433..10750
                     /gene="yabA"
                     /locus_tag="VW144_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEU9601046.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            10753..11622
                     /gene="rsmI"
                     /locus_tag="VW144_001629"
     CDS             10753..11622
                     /gene="rsmI"
                     /locus_tag="VW144_001629"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU9601047.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFDS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETSLKGECLLIVEGASQDVEEKDEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            11849..12367
                     /locus_tag="VW144_001630"
     CDS             11849..12367
                     /locus_tag="VW144_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEU9601048.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENIHSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            12476..12613
                     /locus_tag="VW144_001631"
     CDS             12476..12613
                     /locus_tag="VW144_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEU9601049.1"
                     /translation="MDFEYFYNREAKRFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     K"
     gene            12620..>13094
                     /locus_tag="VW144_001632"
     CDS             12620..>13094
                     /locus_tag="VW144_001632"
                     /inference="COORDINATES: protein motif:HMM:NF018653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEU9601050.1"
                     /translation="MSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVK
                     KGIGLIERVRLGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQ
                     EVKNLDSNYIENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLENY"
BASE COUNT         4015 a         2211 c         3049 g         3819 t
ORIGIN      
        1 ctcaactaat ctaaacaata acggaggaaa tatatcatga ttttaatgac aaaaaatata
       61 aatctaacaa atgaagaatt agagctgata caaggtggag cagatcccta tggtaaagaa
      121 cctaatggtt attatccttg gaaaatggaa ccagtattaa ctctgctggt tcatggattt
      181 tgtcccagag acaccgatga tttaggatat attggaggag gtaatcatct ttgcaaagga
      241 agtgctgcga gattttaagt aaaatttatt agtaatatga agaaacaagt ggagaaagca
      301 gagaatttaa tatgaaaaaa cgggctattc aaattttact agcattgtcc ttaatttttt
      361 acaaatcaac ttggttttgg aggcttttca attatctcgc aaagccctat ctaccagcaa
      421 gtcgtgaatt ttttcagatt ctgcttttga tggagagtgg agttctttta ttagcggtca
      481 tctatctact agtttttgca ggaaagaaaa cttttcattt caagtggcag ctgaggtatt
      541 ttatctacct tttactgggt tacatcactt cgtatatgtc tgactttctc ttttcgtatt
      601 tcatatccct gtcttcaaat caaatttctt tgaatgaaac gatagaaatg atagggagac
      661 aggaattacc ctattttttg ctcatcgttt gcttcatcgg ccctattgct gaggaattga
      721 tttatcgcgg tgtgcttatg acaacctttt tcaaaaactc gccttggtac ggagatgtct
      781 tgctttctgc tattattttc ggttatattc atatcaattt tgctttaact cctcttgctt
      841 ttttcattta tgctagcgga ggtcttattt tggctctatt gtatcgcatg actaaaaaca
      901 tctactatcc aatactagtt catattctca tcaatatcac tgctttctgg gatgtttggt
      961 tactcctatt ttcaggaagt tagcttacta aaaaatgtcg gaattttccg gcattttctt
     1021 ttttcacaaa tagtcaacgt ttttctttcc gatactgaag tggtgtgtag ccacttattt
     1081 ttttgaattg attttgaaaa taagattggc gtgagaaagg cagatagtga agatagttaa
     1141 gaagaatagg atgttctttt ttcctttttg gaaaacttct aaaatatggt ataataaaaa
     1201 gataaagaag ttgggggtag aagatgaaca ttcaacaatt acgctatgtt gtggctattg
     1261 ccaatagtgg tacttttcgt gaagctgctg aaaagatgta tgttagtcag ccgagtctgt
     1321 ctatttctgt tcgtgatttg gaaaaagagt tgggctttaa gattttccgt cggaccagct
     1381 cagggacttt cttgacccgt cgtgggatgg aattttatga aaaagcgcaa gaattggtta
     1441 aaggatttga tatttttcaa aatcagtatg ccaatcctga agaagaaaaa gatgaatttt
     1501 ccgttgctag ccagcactat gacttcttac caccaactat tacggccttt tcagagcgct
     1561 atcctgacta taagaacttc cgtatttttg aatcaactac tgttcaaata ttagatgaag
     1621 tggcgcaagg gcatagtgag attgggatta tctacctcaa caatcaaaat aaaaagggga
     1681 ttatgcaacg ggttgaaaag ttaggtctgg aggtcatcga attgattcct ttccataccc
     1741 atatttatct ctgtgagggt catcctttag ctcggaaaga ggaattagtc atggaggatt
     1801 tagcggattt accaacggtt cgtttcactc aagagaaaga cgagtacctt tattattcag
     1861 agaactttgt cgataccagc gctagctcac agatgtttaa tgtgacagac cgtgccacct
     1921 tgaatggtat tttggagcgg acggacgcct atgcgacagg ttctggattt ttagatagtg
     1981 acagtgttaa tggcattaca gttattcgtc tcaaggataa cctagataac cgcatggtct
     2041 atgttaaacg tgaagaagtg gagcttagtc aagctgggac tctcttcgta gaagtcatgc
     2101 aagaatattt tgatcaaaag aggaaatcat gaaaaaaaga gcaatagtgg cagtcattgt
     2161 actgctttta attgggctgg atcagttggt caaatcctat atcgtccagc agattccact
     2221 gggtgaagtg cgctcctgga ttcccaattt cgttagcttg acctacctgc aaaatcgagg
     2281 tgcagccttt tctatcttac aagatcagca gctgttattc gctgtcatta ctctggttgt
     2341 cgtgataggt gccatttggt atttacataa acacatggag gactcattct ggatggtctt
     2401 gggtttgact ttgataatcg cgggtggtct tggaaacttt attgacaggg ccagtcaggg
     2461 ctttgttgtg gatatgttcc accttgactt tatcaacttt gcaattttca atgtggcaga
     2521 tagctatctg acggttggag tgattatttt attgattgca atgctaaaag aggaaataaa
     2581 tggaaattaa aattgaaact ggtggtctgc gtttggataa ggctttgtca gatttgacag
     2641 aattatcacg cagtcttgcg aatgaacaaa ttaaatcagg ccaggtcttg gtcaatggtc
     2701 aagtcaagaa agctaaatac acagtccaag agggtgatgt cgtcacttac catgtgccag
     2761 aaccagaggt attagagtat gtggctgagg atcttccgct agaaatagtc taccaagatg
     2821 aggatgtggc tgtcgttaac aaacctcagg gaatggttgt gcacccgagt gctggtcata
     2881 ccagtggaac cctagtaaat gccctcatgt atcatattaa ggacttgtcg ggtatcaatg
     2941 gggttctgcg tccaggaatt gttcaccgta ttgataagga tacgtcaggt cttctcatga
     3001 ttgctaaaaa cgatgatgcg catctagcac ttgcccaaga actcaaggat aaaaagtctc
     3061 tccgcaaata ttgggcgatt gttcatggaa atctgcctaa tgatcgtggt gtaattgaag
     3121 cgccgattgg ccggagtgaa aaagaccgta agaaacaggc tgtaactgct aaagggaagc
     3181 ctgcagtgac gcgttttcac gtcttggaac gctttggcga ttatagctta gtagagttgc
     3241 aactggagac agggcgcact catcaaatcc gtgtccacat ggcttatatc ggccatccag
     3301 tcgctggtga tgaggtctat ggtcctcgca agactttgaa aggacatgga caatttcttc
     3361 atgccaagac tttaggtttt actcatccga gaacaggtaa gaccttggaa tttaaagcag
     3421 atatcccaga gatttttaag gaaaccttgg agagattgag aaagtaagaa tgaaaaagaa
     3481 attaactagt ttagcacttg taggcgcttt tttaggtttg tcatggtatg ggaatgttca
     3541 ggctcaagaa agttcaggaa ataaaatcca ctttatcaat gttcaagaag gtggcagtga
     3601 tgcgattatt cttgaaagca atggacattt tgccatggtg gatacaggag aagattatga
     3661 tttcccagat ggaagtgatt ctcgctatcc atggagagaa gaaattgaaa cgtcttataa
     3721 gcatgttcta aaagaccgtg tctttcgtcg tttgaaggaa ttgggtgtcc aaaaacttga
     3781 ttttattttg gtgacccata cccacagtga tcatattgga aatgttgatg aattactgtc
     3841 tacctatcca gttgaccgag tctatcttaa gaaatatagt gataatcgta ttactaattc
     3901 tgaacgtcta tgggataatc tgtacggtta tgataaggtt ttacagactg cttcagaaaa
     3961 aggtgtttca gttattcaaa atatcacaca aggggatgct cattttcagt ttggggacat
     4021 ggatattcaa ctctataact atgaaaatga aaccgattca tcaggtgaat taaagaaaat
     4081 ttgggatgat aattccaatt ccttgattag tgtggtaaaa gtcaatggca agaaaattta
     4141 ccttgggggc gacttagata atgttcatgg agcagaagac aagtatggtc ctctcattgg
     4201 aaaagttgat ttgatgaagt ttaatcatca ccatgatacc aacaaatcaa ataccaagga
     4261 tttcattaaa aatttgagtc cgagtttgat tgttcaaact tcctcctcac caattggttc
     4321 gtatgatagc ggtcaagaaa taaataggga atatattagt tggcttgaat caatgggaat
     4381 tcaatatata aatgcagcca gcaaagacta tgatgcaaca gtttttgata ttcgacaaga
     4441 cggtttagta aacatttcaa cttcctacaa gccgattcca agttttcaag ctggttggca
     4501 taagagttca tatggaaact ggtggtatca agcgcctgat tctacaggag agtatgttgt
     4561 cggttggaat gaaattaagg gtgaatggta ttactttaac caaacgggta tcttattaca
     4621 gaatcaatgg aaaaaatgga acaatcattg gttctatttg acagactctg gtgcttctgc
     4681 taaaaattgg aagaaaattg atggaatctg gtattatttt aacaaagaaa atcagatgga
     4741 aattggttgg gttcaagata aagagcagtg gtattatttg gatgttgatg gttctatgaa
     4801 gacaggatgg cttcaatata tggggcaatg gtattacttt gctccatcag gggaaatgaa
     4861 aatgggctgg gtaaaagata aagaaacctg gtactatatg gattctactg gtgtcatgaa
     4921 gacaggtgag atagaagttg ctggtcaaca ttattatctg gaagattcag gagctatgaa
     4981 gcaaggctgg cataaaaagg caaatgattg gtatttctac aagacagacg gttcacgagc
     5041 tgtgggttgg atcaaggaca aggataaatg gtacttcttg aaagaaaatg gtcaattact
     5101 tgtgaacggt aagacaccag aaggttatac tgtggattca agtggtgcct ggttagtgga
     5161 tgtttcgatc gagaaatctg ctacaattaa aactacaagt cattcagaaa taaaagaatc
     5221 caaagaagta gtgaaaaagg atcttgaaaa taaagaaacg agtcaacatg aaagtgttac
     5281 aaatttttca actagtcaag atttgacatc ctcaacttca caaagctctg aaacgagtgt
     5341 aaacaaatcg gaatcagaac agtagtagaa aagaaggttt tagggccttc tttttcctat
     5401 caactctttt ctatttcctg ttattcatgt tataatggat aaatatgaat aatcggagtg
     5461 agactatgaa atacaaacgg attgtcttta aggtgggtac ttcttctctg acaaatgagg
     5521 atggaagttt atcacgtagt aaggtaaagg atattaccca gcagttggct atgctgcacg
     5581 aggctggtca tgagttgatt ttggtgtctt caggtgccat tgcggctggt tttggagcct
     5641 taggatttaa aaagcgtccg actaaaattg ctgataaaca ggcttcagca gcggtagggc
     5701 agggactttt gttggaagaa tatacgacca atcttctctt gcgtcaaatc gtttctgcac
     5761 aaatcttgct gacccaagat gactttgtgg ataagcgtcg ttataaaaat gcccatcagg
     5821 ctttgtcggt tttgctcaac cgtggggcaa ttcctatcat caatgagaat gatagtgtcg
     5881 ttattgatga gctcaaggtt ggggacaatg acactctaag tgctcaagta gcggcgatgg
     5941 tccaagcaga ccttttagtt ttcttgacag atgtggacgg tctctatact ggaaatccta
     6001 attcagatcc aagagccaaa cgcttggaga gaatcgagac catcaatcgt gagattattg
     6061 atatggctgg tggagctggt tcgtcaaacg gaactggggg tatgttaacc aaaatcaagg
     6121 ctgcaactat cgcgacggaa tcaggagttc ctgtttatat ctgctcatcc ttgaaatcag
     6181 attccatgat tgaggcggca gaggagaccg aggatggttc ttactttgtt gctcaagaga
     6241 aggggcttcg tacccagaaa caatggcttg ccttctatgc tcagagtcaa ggttctattt
     6301 gggttgataa aggggctgcg gaagctctct ctcaacatgg aaagagtctt ctcttatctg
     6361 gtatcgttga agcagaagga gtcttttctt acggtgatat cgtgacagta tttgataagg
     6421 aaagtggaaa atcacttgga aaaggacgcg tgcaatttgg agcatctgct ttggaggata
     6481 tgttgcgttc tcaaaaagcc aagggtgtct tgatttaccg tgacgactgg atttccatta
     6541 ctcctgaaat ccaactactt tttacagaat tttagaggta aactatggtg agtagacaag
     6601 aacaatttga acaggtacag gctgttaaaa aatcgattaa cacagctagt gaagaagtga
     6661 aaaaccaagc cttgctagcc atggctgatc acttagtggc tgctactgag gaaattttag
     6721 cggctaatgc cctcgatatg gcagcggcta aggggaaaat ctcagatgtg atgttggatc
     6781 gtctttattt ggatgcagat cgtatagaag cgatggcaag aggaattcgt gaagtggttg
     6841 ccttaccaga tccaatcggt gaagttttag aaacaagtca gcttgaaaat ggtttggtta
     6901 tcacaaaaaa acgtgtagct atgggtgtca tcggtattat ctatgaaagc cgtccaaatg
     6961 tgacgtctga tgcggctgct ttgactctta agagtggaaa tgcggttgtt cttcgtagtg
     7021 gtaaggatgc ctatcaaaca acccatgcca ttgtcacagc cttgaagaag ggcttggaga
     7081 cgactactat tcatccaaat gtgattcaac tggtggagga tactagccgt gaaagtagtt
     7141 atgctatgat gaaggccaag ggctatctag accttctcat tcctcgtgga ggagctggct
     7201 tgatcaatgc agtggttgag aatgccattg tgcctgttat cgagacagga actgggattg
     7261 tccatgttta tgtcgataag gacgcagatg acgacaaggc actgtctatc atcaacaatg
     7321 ccaaaaccag tcgtccttct gtctgcaatg ccatggaggt tctgctggtt catgaagaca
     7381 aggcagcaag cttccttcct cgcttggagc aagtgctggt tgcagatcga aaagaagctg
     7441 ggttggaacc aattcaattc cgcttggata gcaaagcaag ccagtttgtt tcaggtcaag
     7501 cagctgagac ccaagacttt gacaccgagt ttttagacta tgtccttgct gttaaggttg
     7561 tgagcagttt agaagaagcg gttgcgcaca ttgaatccca cagcacccat cattcggatg
     7621 ctattgtgac ggaaaatgct gaagctgcag catactttac agatcaagtg gactctgcag
     7681 cggtgtatgt taatgcctca actcgtttca cagatggagg acaatttggt cttggttgtg
     7741 aaatggggat ttctactcag aaattgcacg cgcgtggtcc aatgggcttg aaagagttga
     7801 ccagctacaa gtatgtggtt gctggtgatg ggcagataag ggagtaagag atgaagattg
     7861 gatttatcgg tttggggaat atgggtgcta gcttggcaaa atctgtcttg cagactagga
     7921 cgtcagatga gattctcctt gccaatcgta gtcaagctaa ggtagatgct ttcattgcag
     7981 actttggtgg tcaggcttcc agcaatgaag aaatgtttgc agaagcagat gtgatttttc
     8041 taggagttaa gcctgctcag ttttctgaac tgctttctca ataccagacc atccttgaaa
     8101 aaagagaaag tcttcttttg atttcgatgg cagctggatt gaccttagaa aaactagcaa
     8161 gtcttatccc aagtcaacac cgaattattc gtatgatgcc taatacccct gcttctatcg
     8221 ggcaaggagt gattagttat gccttgtctc ctaattgcag ggctgaggac agtgagctct
     8281 tttgtcagct tttatccaag gctggtctct tggttgaatt aggagaaagc ttaatcaatg
     8341 cagcgacagg tcttgcaggc tgtggaccag cctttgtcta tctctttatt gaagccttgg
     8401 cagatgcagg tgttcagacg ggattatcac gagaaacagc cttgaaaatg gcagcccaaa
     8461 ctgtggtagg agctgggcaa ttggtcctag aaagccagca acatcctgga gttttgaaag
     8521 atcaagtttg tagtccaggc ggttcgacta ttgctggcgt agcaagccta gaagcgcatg
     8581 cttttcgagg cacagtcatg gaggcagttc atcaagccta taaacgaaca caagaactag
     8641 gtaaataaga ggtagtttta tctgcctctt ttatggtggc tgatactctt cgaaaatcaa
     8701 attcaaactg cgtcagcttc accttgccgt actcaagtac agcctgcggc tagcttccta
     8761 gtttgctctt tgattttcat tgagtatgaa atgagaagac aaaaagattg tcacaaaccc
     8821 ctattttttt gatagaatag aagtagtaaa aaagaaatga gttagacatg tcaaaaggat
     8881 ttttagtctc tcttgaggga ccagagggag caggcaagac cagtgtttta gaggctctgc
     8941 taccaatttt agaggaaaaa ggagtagagg tgttgacgac ccgtgaacct ggcggagtct
     9001 tgattgggga gaagattcgg gaagtgattt tggatccaag tcatactcag atggatgcta
     9061 aaacagagct acttctctat attgccagtc gcagacagca tttggtggaa aaagttcttc
     9121 cagcccttga agctggcaag ttggtcatca tggatcgttt tatcgatagt tctgttgcct
     9181 atcagggatt tggtcgtggc ttagatattg aagccattga ctggctcaat cagtttgcga
     9241 cagatggcct caaacccgat ttgacactct attttgacat cgaggtggaa gaagggctgg
     9301 ctcgtattgc tgctaatagt gaccgcgagg ttaatcgttt ggatttggaa gggttggact
     9361 tgcataaaaa agttcgtcaa ggctaccttt ctcttctgga taaagaggga aatcgcattg
     9421 tcaagattga tgctagtctc cctttggagc aagttgtgga aactaccaag gctgtcttgt
     9481 ttgacggaat gggcttggcc aaatgaaaca agatcaacta aaggcttggc aaccagctca
     9541 gtttgaccgt tttgtccgta tcttagaaca agaccagctc aatcacgcct atctcttttc
     9601 aggtttcttt ggaagcttgg aaatagcgca atttttagct aagagcctct tttgtacgga
     9661 taaagttggc gtcttaccat gtgagaaatg ccgaagttgc aagctgattg aacaggaaga
     9721 gtttccagat gtcaccttga ttaagccagt caatcaggtc atcaagacag aacgcattcg
     9781 ggaattggtg ggacagtttt ctcaagcagg gattgaaagc cagcaacagg tctttatcat
     9841 cgagcaagcg gataaaatgc atcccaacgc agccaattct ctgctcaagg tcatcgaaga
     9901 accccagagt gaagtttata ttttcttctt gactagcgat gaggaaaaga tgttaccgac
     9961 aatccgaagt cggactcaga tcttccactt taaaaagcaa gaagaaaaac ttatcttact
    10021 cttagaacaa atgggacttg ttaagaaaaa agcgactctt ttagctaagt ttagtcaatc
    10081 gcgagctgaa gcagaaaagt tggctaatca ggcaagtttt tggaccttgg tcgatgaaag
    10141 tgaacgcctg ctgacttggt tagtagctaa gaaaaaagaa agttatctac aggttgccaa
    10201 attagccaac ttggcagatg ataaggaaaa acaggatcag gttttacgga ttcttgaagt
    10261 tctctgtggg caggacctct tgcaggtaag agtaagagtg attctacaag atttactaga
    10321 agctagaaaa atgtggcaag ctaatgtcag ctttcaaaat gccatggaat atctggtctt
    10381 gaaagaaata taaactcaaa aatgaatgat aaagaaagga aagggctgtt ttatggacaa
    10441 aaaagaatta tttgacgcgc tggatgattt ttcccaacag ttattggtaa ccttggccga
    10501 tgtggaagcc atcaagaaaa atctcaagag cctggtagag gaaaatacag ctcttcgttt
    10561 ggaaaattct aagttgcgag aacgcttggg tgaggtggaa gcagatgctc ctgtcaaggc
    10621 caagcatgtt cgtgaaagtg tccgtcgcat ttaccgtgat ggatttcacg tatgtaatga
    10681 tttttatgga caacgtcgag agcaggacga agaatgtatg ttctgtgatg agttgttata
    10741 cagggagtag gcatgcaaat tcaaaaaagt tttaaggggc agtctcccta tggcaagctg
    10801 tatctagtgg caacgccgat tggcaatcta gatgatatga cttttcgtgc tatccagacc
    10861 ttgaaagaag tggactggat tgctgctgag gatacgcgca atacaggtct tttgctcaag
    10921 cattttgaca tttccaccaa gcagatcagt tttcatgagc acaatgccaa ggaaaaaatt
    10981 cctgatttga ttggtttctt gaaagcaggg caaagtattg ctcaggtctc tgatgccggt
    11041 ttgcctagca tttcagaccc tggtcatgat ttggttaagg cagctattga ggaagaaatt
    11101 gcagttgtta cagttccagg tgcctctgca ggaatttccg ccttgattgc cagtggttta
    11161 gcgccgcagc cacatatctt ttacggcttt ttaccgagaa aatcaggcca gcaaaagcaa
    11221 tttttcgact cgaaaaaaga ctatcctgaa actcagattt tttatgaatc gccccatcgt
    11281 gtggcagata cattggaaaa tatgctagaa gtctacggtg accgctccgt tgtcttggtc
    11341 agggaattga ccaaaatcta tgaagaatac caaagaggta caatttctga attgctggaa
    11401 agcattgctg aaacgtctct caagggtgaa tgtcttctga ttgttgaagg tgccagtcag
    11461 gatgtggaag aaaaagacga ggaagacttg ttcttagaaa tccaagctcg tatccagcaa
    11521 ggtatgaaga aaaatcaagc tattaaggaa gtcgctaaga tttaccagtg gaataagagt
    11581 caactctacg ctgcctacca cgactgggaa gaaaaacaat aaagggagac aggatgtaat
    11641 aattctgtct gtttctgttt aacttaatta gtgatgataa tataaagatg tatcacttgg
    11701 tatagaagct ttggtattaa gttttttatt aagcccatac ggaataccga tggttggagc
    11761 agcagttata gcgttcttag aaggtataaa tagaaaaata aggtcatttt aaatcaaagg
    11821 attgataaat cagaaagaag gtgatttttt gcgaacatac gaaaataaag aagaactaaa
    11881 agctgagata gagaaaacat ttgagaaata tattttagaa tttgataata ttccagaaaa
    11941 tttaaaagat aagagagctg atgaagttga cagaactcca gcagaaaacc ttgcttatca
    12001 ggttggttgg accaacttgg ttcttaaatg ggaagaagat gaaagaaagg ggcttcaagt
    12061 aaaaacacca tcggataaat ttaaatggaa tcaacttggt gaattatatc agtggttcac
    12121 agatacctac gctcatttat ctctgcaaga gttgaaagca aaattaaatg aaaatattca
    12181 ttctatctct gcaatgattg attcgttgag tgaggaagaa ttatttgaac cgcatatgag
    12241 aaagtgggct gatgaagcga ctaaaacagc gacttgggaa gtgtataagt ttattcatgt
    12301 aaatacggtt gcaccttttg gaactttcag aactaaaatc agaaaatgga agaagatagt
    12361 attataaatt atatttttaa ctttaaaaaa tttcataaaa atggttacca aaggcgatag
    12421 aagaaaaact atcgtctttt tctttgcaaa tttttaagaa gggaggtgat cttgcatgga
    12481 ctttgaatat ttttataaca gagaagcgaa aagatttaac ttcttaaaag taccggagat
    12541 attagttgat agagaagaat ttcggggctt atcagcagaa gcaattatcc tttattccat
    12601 acttcttaaa tagacaggaa tgtcatttaa gaataactgg atagacaagg aaggcagagt
    12661 atttatctat tttactgtcg aagaaattat gaaaagaaga aatatctcaa agccaactgc
    12721 cataaaaaca ttagatgagc ttgatgtaaa aaaaggaata ggactgatcg aaagagtaag
    12781 gcttggactt ggtaagccga acatcattta tgttaaagac tttatgagta tatttcaggt
    12841 aaaagaaaat gacttacaga agtcaaaaaa cttaacttca gaagtaaaag attttaacct
    12901 cagaagtaaa gaaaatgaac ttcaagaggt taagaacctt gactctaact atatagagaa
    12961 taataagagt aagtatagta agagagaata tagttttggt gaaaacggac ttggaacatt
    13021 tcaaaatgtg tttttagctg ctgaagatat atcggattta caaatcataa tgaactcaca
    13081 gcttgagaat taca
//
LOCUS       DAVKJJ010000050        12832 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000050 DAVKJJ010000000
VERSION     DAVKJJ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12832
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.050"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            175..2466
                     /gene="pcrA"
                     /locus_tag="VW144_001633"
     CDS             175..2466
                     /gene="pcrA"
                     /locus_tag="VW144_001633"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEU9601051.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSVLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            2509..3189
                     /gene="radC"
                     /locus_tag="VW144_001634"
     CDS             2509..3189
                     /gene="radC"
                     /locus_tag="VW144_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU9601052.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(3186..3875)
                     /locus_tag="VW144_001635"
     CDS             complement(3186..3875)
                     /locus_tag="VW144_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9601053.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGKPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(3893..4534)
                     /locus_tag="VW144_001636"
     CDS             complement(3893..4534)
                     /locus_tag="VW144_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEU9601054.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(4617..4832)
                     /locus_tag="VW144_001637"
     CDS             complement(4617..4832)
                     /locus_tag="VW144_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9601055.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGKKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(4840..5187)
                     /locus_tag="VW144_001638"
     CDS             complement(4840..5187)
                     /locus_tag="VW144_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEU9601056.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(5192..6307)
                     /locus_tag="VW144_001639"
     CDS             complement(5192..6307)
                     /locus_tag="VW144_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9601057.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLKAMH
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(6317..7276)
                     /locus_tag="VW144_001640"
     CDS             complement(6317..7276)
                     /locus_tag="VW144_001640"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9601058.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(7389..7958)
                     /locus_tag="VW144_001641"
     CDS             complement(7389..7958)
                     /locus_tag="VW144_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEU9601059.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTLDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDMTDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            8086..8757
                     /locus_tag="VW144_001642"
     CDS             8086..8757
                     /locus_tag="VW144_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEU9601060.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRSITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            8741..9559
                     /locus_tag="VW144_001643"
     CDS             8741..9559
                     /locus_tag="VW144_001643"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEU9601061.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            9556..10452
                     /locus_tag="VW144_001644"
     CDS             9556..10452
                     /locus_tag="VW144_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9601062.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIYIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            10496..11470
                     /gene="pta"
                     /locus_tag="VW144_001645"
     CDS             10496..11470
                     /gene="pta"
                     /locus_tag="VW144_001645"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEU9601063.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(11731..12832)
                     /locus_tag="VW144_001646"
                     /pseudo
     CDS             complement(11731..12832)
                     /locus_tag="VW144_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3744 a         2436 c         2764 g         3888 t
ORIGIN      
        1 tgaggtattg agctagttta tgaaaaaatt gggtgaaaaa gtcgagtgtt ttagaaaccc
       61 acagtgtagt attctagttt caatccacta tattttgcta ctccccgtaa agtttctatt
      121 ttccctgatt tctgatataa tagaaatatt gacttcaaga gtaaggaaga gaagatgaac
      181 gcattattaa atggaatgaa tgaccgtcag gctgaggcgg tgcaaacgac agaaggtccc
      241 ttgctaatca tggcaggggc tggttctgga aagactcgtg ttttgaccca ccgtatcgct
      301 tatttgattg atgaaaagct ggtcaatcct tggaatatct tggccattac ctttaccaac
      361 aaggctgcgc gtgagatgaa agagcgtgct tatagcctca atccagcgac tcaggactgt
      421 ctgattgcga ccttccactc catgtgtgtg cgtattttgc gtcgcgatgc ggaccatatt
      481 ggctacaatc gtaattttac aattgtggat cctggtgaac agcgaacgct catgaaacgt
      541 attctcaaac agttgaactt ggatcctaaa aaatggaatg aacgaactat tttggggacc
      601 atttccaatg ctaagaatga tttgattgat gatgttgctt atgctgccca agctggcgat
      661 atgtatacgc aaattgtggc ccagtgttat acagcctatc aaaaagaact tcgtcagtct
      721 gaatccgttg actttgatga tttgattatg ctgaccttgc gtctctttga tcaaaatcct
      781 gatgttttga cctactacca gcaaaaattc caatacatcc acgttgatga gtaccaagat
      841 accaaccacg ctcagtacca attagtcaaa ctcttggctt cccgttttaa aaatatctgt
      901 gtggttgggg atgcggacca gtctatctac ggttggcgtg gtgctgatat gcagaatatc
      961 ttggactttg aaaaggatta ccccaaagcc aaggttgttt tgttggagga aaattaccgc
     1021 tcaaccaaaa ccattctcca agcggccaac gaggttatta aaaataataa aaatcgccgt
     1081 cctaaaaatc tctggactca aaacgctgat ggggagcaaa tcgtttacta tcgtgccgat
     1141 gatgagctgg atgaggctgt atttgtagcc agaaccatcg atgaacttag tcgcagtcaa
     1201 aacttccttc ataaggattt tgcagttctc tatcggacta atgcccagtc ccgtacaatt
     1261 gaggaagccc tgctcaagtc taacattcct tataccatgg ttggcggaac caaattctac
     1321 agccgtaagg aaattcgcga tattattgct tatctcaacc ttattgctaa tttgagtgac
     1381 aatattagtt ttgagcgtat tatcaacgag cctaaacgtg gaattggtct aggtacagtt
     1441 gagaaaatcc gtgattttgc aaatttgcaa aatatgtcta tgctggatgc ttctgctaat
     1501 attatgttgt ctggtatcaa gggtaaggca gcccaatcta tctgggattt tgccaatatg
     1561 atgcttgatt tgcgggagca gctagaccac ttaagcatta cagagttggt tgagtccgtc
     1621 ctagaaaaaa caggttatgt cgatattctt aacgcccaag cgactctaga aagcaaggca
     1681 cgggttgaaa atatcgaaga gtttctttct gttacgaaga actttgatga caccactgat
     1741 gtgacagaag aggaaactgg tctggacaaa ctgagtcgtt tcttaaatga cttggctttg
     1801 attgccgaca cagattcagg tagtcaggag acatcagaag tgaccttgat gaccctgcat
     1861 gctgccaaag gtctcgaatt tccagttgtc tttttgattg ggatggaaga aaatgtcttt
     1921 ccacttagtc gtgcgactga agatccagat gaattagaag aagagcgccg tctagcctat
     1981 gtaggtatca cgcgtgcaga gaaaattctc tatctgacca atgccaactc acgcttgctt
     2041 tttggtcgta ccaattataa ccgtccgact cgttttatta acgaaatcag ttcagacttg
     2101 cttgagtatc aaggtctggc tcgtcctgca aatacaagct ttaaggcatc atatagcagt
     2161 ggtagtattt cctttggtca aggtatgagt ttggctcagg ctcttcaaga ccgtaaacgc
     2221 ggtgctgccc caaaatcaat ccagtcaagc gttcttccat ttggtcaatt tacagctggc
     2281 gcaaaatcag catctagcga ggcaaattgg tccattggtg acattgctct ccacaagaaa
     2341 tggggagagg gaactgttct ggaagtttca ggtagcggtg ctaggcagga attgaaaatc
     2401 aatttcccag aagtaggttt gaaaaaactt ttagccagtg tggctccaat tgagaaaaaa
     2461 atctaatttt ccatccttct cacgaataat aaagtgagga ggatttttat gtacagtatt
     2521 tcattccaag aagattcact attaccaaga gaaaggctgg ccaaggaagg agttgaagcg
     2581 cttagtaacc aagaattgct agctatttta ctcaggacag gaacacgtca agctagcgtt
     2641 tttgaaattg cccaaaaagt cttgaacaat ctttcaagcc taacggattt gaaaaaaatg
     2701 accctgcagg aattgcagag tttgtctggt attgggcgtg ttaaggccat agaattacaa
     2761 gctatgattg aactggggca tcgtattcac aaacacgaga ctcttgaaat ggaaagtatt
     2821 ctcagcagtc aaaagttggc caagaagatg cagcaggaat taggggataa aaaacaagag
     2881 cacctggtgg cactctatct caatactcaa aatcaaatca tccatcagca gaccattttt
     2941 atcggttctg taactcgtag tatcgctgaa ccgcgagaga ttctgcacta tgcaatcaag
     3001 catatggcga cttctcttat cttggtccac aatcatcctt caggagcggt agcgcctagc
     3061 caaaatgatg atcatgtcac taaacttgtt aaagaagcct gcgaactgat ggggattgtt
     3121 ctcttggacc atttgattgt ctctcattct aattacttta gttatcgtga aaagacagat
     3181 ttaatctaaa gttcattaac gacatagtca aagagtttct tatctttggg acgattttca
     3241 aaaagaagtt ctggatgcca ttggacaccg agaaaggcga catcatccgt actcatgaca
     3301 gcctcaatga taccatcttt aggatcatga gccacaactt ttaaatttgg tgctaagtcc
     3361 ttgatgctct ggtggtggaa ggagttgata tgagagattt ctccatagat ttcttggaga
     3421 acggtatctg gttctgttac caagcgttga gttgtgtact caacagaaga atcctgccaa
     3481 tggtcttcga tatcttggta caaagttcca cccatggcaa cgttaaagag ttgggtacca
     3541 cggcagacag agaaaatggg ctttttctgt ttaatagctt ccttgatgag ggccagttcg
     3601 aagatatctc tttgaaggtg ataatcatcg ctatcaatgg ttttgggttt gccatagaat
     3661 tttggatcga cattttgccc acctgtcaag atgagcttgt caatcaaact gatatagtgg
     3721 caggccattt cttgatcacc aatcggtagg atgataggaa tccctccagc atctttaacg
     3781 ccttcaacaa agccttttgc tgcgtagctc atcatgatgt catcatctgg atgagttttt
     3841 tcgtttcctg taatcccaat aactggtttt ttcataaaat gattttcgct ttctaatcct
     3901 cttttcgcat gaagtagagg agggtttgga gttcacttgt caaatcgaca tactgaacga
     3961 ccacgtcttt tggtaaatgc agatggactg gtgaaaaact gagaattcct ttcacaccag
     4021 catcaaccaa gagattagca acctcttgtg acttgacgct gggaacagtt aggatagcag
     4081 tcttcacatc agcatccttg attttatcct tgatctgaga aatcccgtaa atgggaatcc
     4141 cgtcaggagt ttgggtaccg acttcaggat ggtcgtctag gtcaaaggcc atgataatct
     4201 tcatcttgtt acgttcgtgg aagcggtagt ggagaagggc atggcccata tttccaatac
     4261 caaccagcat gacattggta atagagttgt cattgagcaa atcggcaaaa aatgtcatta
     4321 attttttgac atcatagcca aaaccacgac gaccaagttc accaaaatag gaaaaatcac
     4381 gacgtacggt cgctgaatca ataccgatag cctctgcaat ttgcttagag ttggcacgtt
     4441 caatcttttc tgcatgaaat ctcttaaaaa ttcgatagta gagagagagt ctttttgctg
     4501 tagcttttgg aatagcaaac tgtttatctt tcacaaaatc acaacctttc tattcttcta
     4561 ttttatagaa acattgtgaa aaaatcaaca aaaataagaa aaaactaaga aaaatcttag
     4621 ttttgatgta aaaaatctgc atgagataga aaacggtaga ggtctccgac cagcccctga
     4681 taaacttttt tgcccctaaa agtcagagaa gtcacataaa gtgtatctgg taaggttaca
     4741 catcctgaca aagtcaacat gagagcctca tgatcctcat acttgagagt acgctctaca
     4801 tgatagcagt ccttataggt cagttcaaac attttggctc tatctttccg attttgtaaa
     4861 gacaccacgt tctaccaagc tatccatgag gaagtagaat ttttcctgat gaatatggtg
     4921 gtcttctgat ttgaaaatat caactagacg aaggccaaac ttgtcagtga tattgatttt
     4981 agcccctgta agttccttgt taatgatgat tttgagttgg aagccttcac cgctgtttgg
     5041 cactttttcc aaaaggcgag tcagttcata gttaccaacc ttagtttcaa aaaaggtgtt
     5101 atctttgagg gtgaattttt taacagaagg gctaagagtg taatcgtaac gacaattttt
     5161 taactgaatg attttttcaa atgccatatg gctaacctcc gataatttct tttaaggttt
     5221 ttgcgagggt ttgtaggtct tcaacggtat tttgtggcga caaactgatg cgaagggatt
     5281 ccttcaagcg ttctgaattt gcgccatgca tggctttaag aacatggctg gattggacaa
     5341 cgcctgcagt acaggctgag ccagtagaga ttgaaattcc agctaaatct agccgaagga
     5401 gtaagaggtc atttttctga ccaggaaatc caatattgag aacataaggg agatgatgtt
     5461 ttcctctatt caagtaatac tgaatgccct ccagctctgc cagaaaggca gtttctagat
     5521 tttgtacatg ttgaaaatgt tcttcttgtt tttctaggtc ttcttttagg gctgcaacca
     5581 tgcctacgat ggcaggcaga ttttcagttc ctgcacgttt tttctgttcc tgatctccgc
     5641 catgtagata ggaatcaaag tccatgctag atgcgtagag aaaaccgatt cccttaggac
     5701 catggaattt gtgggcagaa gcagtgagaa aatcaatgcc caattcttct gaatgaattg
     5761 ggattttacc aatagcctga actgcatcaa catgataggc agcagggtgt tgcttgagta
     5821 tttggccaat ttcagcgatg ggaagtaggt ttcctgtctc attattggca aacatagtag
     5881 aaaccaaaat cgtatcgtca cgtaaagcct tttgaatttg ctgggctgtg atttcttgat
     5941 tttctggctg gataatggtt gcttcaaacc caaagtgttg aaccaagtaa tcaattgttt
     6001 caaggacagc atggtgctcg atggcagttg tgatgatatg ttttccttgt tcttggtgac
     6061 gaagacagta gccaatgatg gtagtattat tgccttcagt cccaccagaa gtgaaaaaga
     6121 tatgttgagg ttttgtcctt agtaactggg ctagttcctg acgggcttct cgcaagagtt
     6181 tgccagcttg acgaccatga ccatgaatac tagaaggatt tccgtgggtt tcttgcataa
     6241 ccttggtcat agctgaaata gcaactgctg acataggagt cgttgcagca ttgtccaaat
     6301 aaatcaaaga atcaccttat ttctttttat tgtaggcaaa gagtgggctg actggttttc
     6361 tttcgtgaat acggacgata gcatcaccaa ttaactcact agcagtgatg tagcatacat
     6421 ttttaggagt tttttctttt gttgctactg aatcagtcac aagaatttct ttaatattag
     6481 tattgtcaag aagctcagca gctccctcga cgaagagacc gtggctagaa acagcataaa
     6541 tttctgtagc tccttcacgt tcaacgattt tagaagcttc agagaaggta cgtcctgtat
     6601 ttaaaatatc atcaatcaag atagctttct taccttcaac atcaccaata atataacctt
     6661 cgttacgagt tgcatcgtct tgagggtagt cgataatggc gataggagca tcaagatatt
     6721 cagccaggct acgcgcacgt ttgacacctg aatttttagg gctaacgaca acaacatctg
     6781 aaccaagcaa tcctttatcg cagtaatgtt ttgcgaatag gggaacagtg aaaagattat
     6841 ccactggaat atcaaagaaa ccttgaacct gaacggcatg caaatcaaga gtcaggatac
     6901 gatcaactcc agccttaacc agcatattgg caactagttt tgctgtaagt ggctcacgag
     6961 gacaagcaat gcggtcttga cgtgcatagc caaaatatgg aaggacaacg ttgatactgt
     7021 gggcacttgc acgcacacaa gcatcgacca tgattaacaa ttccattagg tggttgttga
     7081 cagggaaact tgttgattgg atgatgtaaa catcataacc acggacactt tcttcgatat
     7141 ttacttggat ttctccgtct gaaaattgac gtgatgatag ttttccaagt gggacaccaa
     7201 cagcttgggc aattttttgt gcaatctctt ggttagagtt gagtgcgaaa agtttcatgt
     7261 tttttctatc tgacattata gaccgtcctc tgtaaacttt ataaatccta gttatattta
     7321 ccttacatat atgaactggg atttgtgtat ttttatcttt tctattttac caaaaaatgg
     7381 agattatttc agctattttt catacttttg acaaatcgaa ccaattttga aggagctttt
     7441 tggtaggaaa tctgattttt ctctaaaaat tgtcggaaat cctgtttgcc ttgctcatga
     7501 ttttccactt caagctccaa ttcgtaatct gtcatatcaa agtatcggct ctgatccagt
     7561 gccatgagac caatagctgt tttcatttca tagcgaagcg ttgttagaca accaagaacc
     7621 tgccagttct tactttggat accatgtttc gccaattcat ccagtactag cccttgagga
     7681 agttcttcct tactcagata gttctcagca tcttttagtt gcaatttttg gttgtattcc
     7741 atgtttccaa cactctgcgg gactttgagt gtcaactcag cccagtcttc aaaggttcga
     7801 atgcgcatag cgactttctt ttctcgcagt tcaaaatcaa gcgtgtcgat gtagtaattt
     7861 gtttgaagaa caggagtgac acctgtgaac tggtctttta gacgattgta ttcatctttt
     7921 ttcaatagtg ttttcaattc aatttctaaa tgtttcattt ttcttacctt tttttatcgt
     7981 tgaaagcgga tttatggtat aataagcatt gtatttattg tatatgaatc tggagaaaaa
     8041 atcaaagata tttttgacgg ataatatgag aacaagggag aatatatgac cttagaatgg
     8101 gaagaatttc tagatcctta cattcaagct gttggtgagt taaagattaa acttcgtggt
     8161 attcgtaagc aatatcgtaa gcaaaataag cattctccaa ttgagtttgt gaccggtcga
     8221 gtcaagccaa ttgagagcat caaagaaaaa atggctcgtc gtagcattac ttatgcgacc
     8281 ttggaacacg atttgcagga tattgctggc ttacgtgtga tggttcagtt tgtagatgac
     8341 gtcaaggaag tagtggatat tttgcacaag cgtcaggata tgcgaatcat acaggagcga
     8401 gattacatta ctcatagaaa agcatcaggc tatcgttcct atcatgtggt agtagaatat
     8461 acggttgata ccatcaatgg agctaagact attttggcag aaattcaaat tcgtactttg
     8521 gccatgaatt tctgggcaac gatagaacat tctctcaact acaagtacca aggggatttc
     8581 ccagatgaga ttaagaagcg actggaaatt acagctagaa tcgcccatca gttggatgaa
     8641 gaaatgggtg aaattcgtga tgatatccaa gaagctcagg cactttttga tcctttgagt
     8701 agaaaattaa atgacggtgt aggaaacagt gacgatacag atgaagaata caggtaaacg
     8761 aattgatctg atagccaata gaaaaccgca gagtcaaagg gttttgtatg aattgcgaga
     8821 tcgtttgaag agaaatcagt ttatactcaa tgataccaat ccggatattg tcatttccat
     8881 tggcggggat ggtatgctct tgtcggcctt tcataagtac gaaaatcagc ttgacaaggt
     8941 ccgctttatc ggtcttcata ctggacattt gggcttctat acagattatc gtgattttga
     9001 gttggacaag ctagtgacta atttgcagct agatactggg gcaagggttt cttaccctgt
     9061 tctgaatgtg aaggtctttc ttgaaaatgg tgaagttaag attttcagag cactcaacga
     9121 agccagcatc cgcaggtctg atcgaaccat ggtggcagat attgtaataa atggtgttcc
     9181 ctttgaacgt tttcgtggag acgggctaac agtttcgaca ccgactggta gtactgccta
     9241 taacaagtct cttggcggtg ctgttttaca ccctaccatt gaagctttgc aattaacgga
     9301 aattgccagc cttaataatc gtgtttatcg aacactgggc tcttccatta ttgtgcctaa
     9361 gaaggataag attgaactta ttccaacaag aaacgattat catactattt cggttgacaa
     9421 tagcgtttat tctttccgta atattgagcg tattgagtat caaatcgacc atcataagat
     9481 tcactttgtc gcgactccta gccataccag tttctggaac cgtgttaagg atgcctttat
     9541 cggtgaggtg gatgaatgag gtttgaattt atcgcagatg aacatgtcaa ggttaagacc
     9601 tttttaaaaa agcacgaggt ttctaaggga ttgctggcta agattaagtt tcgaggtgga
     9661 gctattctgg tcaataatca accgcaaaat gcaacgtatc tattggacgt tggagactac
     9721 gttaccattt acattcccgc tgaggaaggc tttgaaacct tggaggctat tgagcatcca
     9781 ttagatattc tctatgagga tgaccacttt ctagtcttga ataaacccta tggagtggct
     9841 tctattccta gtgtcaatca ctctaatacc attgccaatt ttatcaaggg ttactatgtc
     9901 aagcaaaatt atgaaaatca gcaggttcac attgttacca gactagatag ggacacttct
     9961 ggcttgatgc tctttgccaa gcacggttat gcccatgcac gattagacaa gcagttgcag
    10021 aagaaatcta tcgagaaacg ctactttgct ttggttaagg gagatggaca tttggagcca
    10081 gaaggggaaa ttattgctcc gattgcgcgt gatgaagatt ccattattac cagacgagtg
    10141 gctaaaggcg gaaagtatgc ccatacttca tacaagattg tagcttctta tggaaatatt
    10201 cacttggtct atattcacct gcacactggt cgaacccatc aaatccgagt ccatttttct
    10261 catatcggtt ttcctttgct gggagatgat ttgtatggtg gtagtctgga agatggtatt
    10321 caacgtcagg ctctgcattg ccattaccta tccttttatc atccattttt agagcaagac
    10381 ttgcagttag aaagtccctt gccggatgat ttcagtaacc ttattaccca gttatcaact
    10441 aatactctat aaaaactgtc tcagagtata attattatct taaaggagaa aactcatgga
    10501 agtttttgaa agtctcaaag ccaaccttgt tggtaaaaat gctcgtatcg ttctccctga
    10561 aggggaagag cctcgtattc ttcaagcaac aaaacgctta gtaaaagaaa cagaagtgat
    10621 tcctgttttg cttggaaatc ctgaaaaaat taaaatttat cttgaaattg aaggaatcat
    10681 ggatggttat gaggtcatcg accctcaaca ttatcctcaa tttgaagaaa tggtttctgc
    10741 cttggtggag cgtcgcaagg gcaaaatgac tgaagaagat gtacgcaagg ttttggttga
    10801 agatgtcaac tactttggtg tgatgttggt ttacttggga ttggttgatg gaatggtgtc
    10861 aggagcgatt cactcaacag cttcaacagt tcgtccagct ctacaaatca tcaaaactcg
    10921 tccaaatgta actcgtactt caggagcctt cctcatggtt cgtggtacgg aacgttacct
    10981 atttggagac tgtgccatta acatcaatcc agatgcagaa gccttggctg aaattgccat
    11041 caactcagca atcacagcta agatgtttgg catcgaacct aaaattgcca tgttgagcta
    11101 ttctactaaa ggttcagggt ttggtgaaag cgttgataag gtcgttgaag caactaaaat
    11161 tgctcacgac ttgcgtcctg accttgaaat cgatggtgag ttgcaatttg atgcagcctt
    11221 tgttcctgaa actgcagctc tgaaagctcc tggaagtacg gtagctggtc aagcaaatgt
    11281 cttcatcttc ccaggtatcg aggcaggaaa tatcggttac aagatggctg aacgcctggg
    11341 tggctttgcg gctgtaggac ctgttttgca aggtttaaac aagccagtta atgatctttc
    11401 tcgtggatgt aatgcagatg atgtttacaa gttgaccctc atcacagcag ctcaagcagt
    11461 tcatcaatag tgaaaactat aaagtgatat actatgctat actgtagtta tgaaactatg
    11521 tacgaaaagc actgccatta attcctgaga actaaattac tgattggtgt caaaaaggaa
    11581 aacttccaag cgatgatatc ctgtctatac acgacctata gaaatctgta atatacatat
    11641 ccgtaaaacg ataaattccc tttttgattt taaatgagta tgaaaagaga attttttggc
    11701 tctttgtcaa ctgtagtggg ttgaagtcag ctaagctcga gaaaggacaa attttgtcct
    11761 ttcttttttg atattcagag cgataaaaat ccgttttttg aagttttcaa agtttcgaaa
    11821 accaaaggca ttgtgtttga taagtttgat gagattattg gtcgcttcca gtttggcatt
    11881 agaatagtgt agttgaaggg cgttgacaat cttttcttta tctttgagga aggttttaaa
    11941 gacagtctga aaaagaggat gaacctgctt tagattgtcc tcaataagcc cgaaaaattt
    12001 ctctggttcc ttattctgga agtgaaacag caagagttga tagagctgat agtggtgttt
    12061 caagtcttgt gaatagctca aaagcttgtt taaaatctct ttattggtta agtgcatacg
    12121 aaaagtagga cgataaaaac gtttatcact cagtttacgg ctatcctgtt gaatgagttt
    12181 ccagtagcgc ttgatagcct tgtattcatg ggattttcga tgaaactgat tcatgatttg
    12241 gacacgcaca cgactcatag cacggctaag atgttgtaca atgtgaaagc gatcaagaac
    12301 gattttagca ttcgggagtg aaacagcctg ggagactgtt tcagcctgag cctagaaatt
    12361 tgaaagcgaa gctgtttagc caagtcatag taagggctaa acatatccat agtaatgatt
    12421 ttgacgcgac atcggacggc tctattatat ttaagaaagt gatctcggat gacagcttgt
    12481 gttctgcctt caagaacagt gataatatta agattatcaa aatcttgcgc aatgaagctc
    12541 atctccatct cccgattgaa acagtcactc cccggactgt ttcaacctcc caagacataa
    12601 tctcaggcag atttctaaaa ttacactcaa agtggaagtc attgagcttg cgaatgacag
    12661 ttgaagttga aatggccagc tgatgggcaa tatcagtcat agaaattttt tcaattaact
    12721 tttgagcaat tttttggttg atgatacgag ggatttggtg atttttcttt accaggggag
    12781 tctcagcaac catcattttt gaacagtgat agcacttgaa acggcgtttt ct
//
LOCUS       DAVKJJ010000051        12600 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000051 DAVKJJ010000000
VERSION     DAVKJJ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12600
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.051"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            <1..1130
                     /gene="dnaN"
                     /locus_tag="VW144_001647"
     CDS             <1..1130
                     /gene="dnaN"
                     /locus_tag="VW144_001647"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU9601064.1"
                     /translation="FSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGITLIG
                     SNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQNQIV
                     LTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRPILT
                     GVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDDIET
                     VEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERAR
                     LLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDS
                     LKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            1195..1389
                     /locus_tag="VW144_001648"
     CDS             1195..1389
                     /locus_tag="VW144_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEU9601065.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            1473..2588
                     /gene="ychF"
                     /locus_tag="VW144_001649"
     CDS             1473..2588
                     /gene="ychF"
                     /locus_tag="VW144_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU9601066.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRASYHLLGLGTYFTAGEKEVRAWTFKRGMKATQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2659..3228
                     /locus_tag="VW144_001650"
     CDS             2659..3228
                     /locus_tag="VW144_001650"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU9601067.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            3229..6738
                     /gene="mfd"
                     /locus_tag="VW144_001651"
     CDS             3229..6738
                     /gene="mfd"
                     /locus_tag="VW144_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU9601068.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERELAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKEIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIVENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKESKEENSI"
     gene            6796..7062
                     /locus_tag="VW144_001652"
     CDS             6796..7062
                     /locus_tag="VW144_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU9601069.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            7055..7423
                     /locus_tag="VW144_001653"
     CDS             7055..7423
                     /locus_tag="VW144_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU9601070.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            7543..8811
                     /locus_tag="VW144_001654"
     CDS             7543..8811
                     /locus_tag="VW144_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEU9601071.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMAYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            8808..10085
                     /gene="tilS"
                     /locus_tag="VW144_001655"
     CDS             8808..10085
                     /gene="tilS"
                     /locus_tag="VW144_001655"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU9601072.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEEEDEFVLHYQNQVAYQGYLFSFGLPLEG
                     ESIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            10089..10631
                     /gene="hpt"
                     /locus_tag="VW144_001656"
     CDS             10089..10631
                     /gene="hpt"
                     /locus_tag="VW144_001656"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU9601073.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKAREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            10647..>12600
                     /gene="ftsH"
                     /locus_tag="VW144_001657"
     CDS             10647..>12600
                     /gene="ftsH"
                     /locus_tag="VW144_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU9601074.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMFGAQNPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDE"
BASE COUNT         4339 a         1892 c         2562 g         3807 t
ORIGIN      
        1 atttttcaat taataaaaat ttatttctac aagcattaaa tactactaag agagctatta
       61 gttctaaaaa tgccattcct attttatcaa cagtaaaaat tgacgtgacc aatgaaggta
      121 ttactttaat tggttcaaat ggtcaaattt caattgaaaa ttttatttct caaaaaaatg
      181 aagatgctgg tttgttaatt acttctttag gttcgatcct tcttgaagct tctttcttta
      241 tcaatgtagt atctagttta cctgatgtaa ctcttgattt taaagaaatt gaacaaaatc
      301 aaattgtttt aaccagtggc aaatcagaaa ttaccctaaa aggaaaagat agcgaacaat
      361 atccacgaat ccaagaaatt tcagcaagca ctcctttaat acttgaaaca aaattactca
      421 agaaaattat taatgaaaca gcctttgctg caagtacaca agagagtcgt ccgattttaa
      481 caggtgtcca cttcgtattg agtcaacaca aagagttaaa aacagttgca acagactctc
      541 atcgcctaag ccagaaaaaa ttgactcttg aaaaaaatag tgatgatttt gatgtcgtaa
      601 ttcctagccg ttctctacgc gaattttcag cggtatttac agatgatatc gaaactgtag
      661 agattttctt tgccaataac caaatcctct ttagaagcga aaatattagc ttctatactc
      721 gtctcctaga aggaaactat cctgatacag atcgcttgat tccaacagac tttaacacta
      781 ctattacttt taatgtggta aacttacgcc agtcaatgga gcgtgcccgt cttttatcaa
      841 gtgcgactca aaatggtact gtgaaacttg aaattaagga tggggttgtt agcgcccatg
      901 ttcactctcc agaagttggt aaagtaaacg aagaaatcga tactgatcag gttactggtg
      961 aagatttgac cattagtttc aacccaactt acttgattga ttctcttaaa gctttaaata
     1021 gcgaaaaggt gaccattagc tttatctcag ctgttcgtcc atttactctt gtaccagcag
     1081 atactgacga agacttcatg cagctaatta caccagttcg tacaaattaa gtgaaagagg
     1141 ttgagcctgg ctcgcctctt ttatgatata atcgaaaaag aaaaggagag tagtatgtat
     1201 caagttggaa attttgttga gatgaaaaaa tcacacgctt gtacaatcaa gtcaactggt
     1261 aaaaaggcta atcgttggga aattacacgt gtaggagcag atatcaaaat aaaatgcagt
     1321 aattgtgagc atgttgtcat gatggggcga tatgattttg agcgaaaaat gaataaaatt
     1381 attgactgag aacccttagt tagagggtta gcactttatc cctttttgtg ttataatatt
     1441 agggattgaa atgaaaacgg agaatgagaa atatggcttt gacagcaggt atcgttggtt
     1501 tgccaaacgt tggtaaatca acactattta atgcaattac aaaagcagga gcagaggcag
     1561 caaactaccc atttgcgact attgatccaa atgttggaat ggtggaagtt ccagatgaac
     1621 gcctacaaaa actaactgaa atgataactc ctaaaaagac agttcccaca acatttgaat
     1681 ttacggatat tgcagggatt gtaaaaggag cttcaaaagg agaagggcta gggaataaat
     1741 tcttggccaa tattcgtgaa gtagatgcga ttgttcacgt agttcgtgct tttgatgatg
     1801 aaaatgtgat gcgcgagcaa ggacgtgaag acgcctttgt agatccactt gcagatattg
     1861 atacaattaa tctggaatta attcttgctg acttagaatc agtgaacaaa cgatatgcgc
     1921 gtgtagaaaa gatggcacgt acgcaaaaag ataaagaatc agtagcagaa ttcaatgttc
     1981 ttcaaaagat taaaccagtc ctagaagacg ggaaatcagc tcgtaccatt gaatttacag
     2041 atgaggaaca aaaggttgtc aaaggtcttt tccttttgac gactaaacca gttctttatg
     2101 tagctaatgt ggacgaggat gtggtttcag aacctgactc tatcgactat gtcaaacaaa
     2161 ttcgtgaatt tgcagcgaca gaaaatgctg aagtagtcgt tatttctgcg cgtgctgagg
     2221 aagaaatttc tgaattggat gatgaagata aaaaagagtt tcttgaagcc attggtttga
     2281 cagaatcagg tgtagataag ttgacgcgtg catcttacca cttgcttgga ttgggaactt
     2341 acttcacagc tggtgaaaaa gaagttcgcg cttggacttt caaacgtggt atgaaggcta
     2401 ctcaagcagc tggtattatc cactcagact ttgaaaaagg ctttattcgt gcagtaacca
     2461 tgtcatatga agatctagtg aaatacggat ctgaaaaggc cgtaaaagaa gctggacgct
     2521 tgcgtgaaga aggaaaagaa tatatcgttc aagatggcga tatcatggaa ttccgcttta
     2581 atgtctaaaa attaataaat ggtgtcaatt aggttggaaa aaaattccaa cccttttggc
     2641 ttttgaaagg aaaaataaat gaccaaatta cttgtaggct tgggaaatcc aggggataaa
     2701 tattttgaaa caaaacacaa tgttggtttt atgttgattg atcaactagc gaagaaacag
     2761 aatgtcactt ttacacacga taagatattt caagctgacc tagcatcctt tttcctaaat
     2821 ggagaaaaaa tttatctggt taaaccaacg acctttatga atgaaagtgg aaaagcagtt
     2881 catgctttat taacttacta tggtttggat attgacgatt tacttatcat ttacgatgat
     2941 cttgacatgg aagttgggaa aattcgttta agagcaaaag gctcagcagg tggtcataat
     3001 ggtatcaagt ctattattca acatatagga actcaggtct ttaaccgtgt taagattgga
     3061 attggaagac ctaaaaatgg tatgtcagtt gttcatcatg ttttgagtaa gtttgacagg
     3121 gatgattata tcggtatttt acagtctgtt gacaaagttg acgattctgt aaactactat
     3181 ttacaagaga aaaattttga gaaaacaatg cagaggtata acggataaat ggtgacctta
     3241 ttagatttat tctcagaaaa tgatcagatt aaaaaatggc atcaaaattt aacagataag
     3301 aaaagacaac taatacttgg tttatcaaca tctactaagg cgcttgcaat tgcaagcagt
     3361 ttagaaaaag aagataggat tgtgttattg acgtcaactt atggagaagc agaaggactt
     3421 gttagtgatc ttatatctat cttgggtgag gaactcgtct atccattttt ggtagatgat
     3481 gctcctatgg tggagttttt gatgtcttca caggaaaaaa ttatttcacg ggttgaagcc
     3541 ttgcgttttt tgactgattc atctaagaaa gggattttag tttgtaatat cgcagcaagt
     3601 cgattgattt taccgtctcc caatgcattc aaagatagta ttgtaaaaat ctcagttggt
     3661 gaagaatatg atcaacacgc gtttatccat cagttaaagg aaaatggcta tcgaaaagtt
     3721 actcaagtac aaactcaggg cgaatttagt cttcgaggag atattttaga tatttttgaa
     3781 atatcccagt tagaaccttg tcgaattgag ttttttggtg atgaaattga tggtatcagg
     3841 tcatttgaag tagaaacaca attatcgaaa gaaaataaga cagaactcac tatctttcca
     3901 gctagtgata tgcttttgag agaaaaggat tatcaacgag gacagtcagc tttagaaaaa
     3961 caaatttcaa aaactttatc acctattttg aaatcatacc tagaagaaat tctttcaagt
     4021 tttcatcaaa agcaaagtca tgcagattct cggaagtttt tatctttgtg ctatgataag
     4081 acatggactg tctttgatta tattgaaaaa gatactccaa tattctttga tgattatcaa
     4141 aaattgatga atcagtatga agtatttgaa agagaattag cgcagtactt tacagaagaa
     4201 ttacagaata gtaaagcatt ttctgatatg cagtattttt ctgatattga acaaatctat
     4261 aaaaaacaaa gtccagtgac ctttttctct aatcttcaaa agggtttagg aaatctcaaa
     4321 tttgacaaaa tttatcaatt caatcaatat cctatgcagg aatttttcaa tcagttttct
     4381 tttctaaaag aagaaattga acgatataaa aaaatggatt acaccattat tctgcagtct
     4441 agcaattcaa tgggaagtaa aacattggag gatatgttag aggaatatca gattaaattg
     4501 gattctagag ataagacaag tatctgtaaa gaatctgtaa atttaataga gggtaatctc
     4561 agacatggtt ttcattttgt agatgaaaag attttattga taactgaaca tgagattttt
     4621 caaaagaaat taaagcgtcg ttttcgaaga caacatgttt caaatgcaga gagattaaaa
     4681 gattacaatg aacttgaaaa aggggactat gttgtccatc atatccatgg gattggtcaa
     4741 tatctaggaa ttgaaaccat tgaaatcaag gaaattcatc gtgattatgt cagtgtccag
     4801 taccaaaatg gtgatcaaat ttctattccc gtggaacaga ttcatctact gtccaaatat
     4861 atttcaagtg atggtaaagc tccaaaactc aataaattaa atgacggtca ttttaaaaag
     4921 gccaagcaaa aggttaagaa ccaggtagag gatatagctg atgatttaat caaactctac
     4981 tctgaacgta gtcagttgaa gggttttgct ttctcagctg atgatgatga tcaagatgcc
     5041 tttgatgatg ctttccctta tgttgaaacg gatgatcaac ttcgtagtat tgaggaaatc
     5101 aagagggata tgcaggcttc tcagccaatg gatcgacttt tagttgggga tgttggtttt
     5161 ggaaagactg aagttgctat gcgtgcagcc tttaaagcag tcaatgatca caaacaggtt
     5221 gtcattctag ttccgacgac ggttttagcg caacagcact atacgaattt taaggaacga
     5281 ttccaaaatt ttgcagttaa tgttgatgtg ttgagtcgct ttagaagtaa aaaagagcag
     5341 actgcaacac ttgaaaaatt gaaaaacggt caagtcgata ttttgattgg aacacatcgt
     5401 gttttgtcaa aagatgttgt gtttgctgat ttgggcttga tgattattga tgaggaacag
     5461 cgatttggtg tcaagcataa ggaaactttg aaagaactga agaaacaagt ggatgtccta
     5521 accttgaccg ctacgccaat ccctcgtacc ctccatatgt ctatgctggg aatcagagat
     5581 ttgtctgtta ttgaaactcc gccgactaat cgctatcctg ttcagaccta tgttttggaa
     5641 aagaatgata gtgtcattcg tgatgctgtc ttgcgtgaaa tggagcgtgg aggtcaagtt
     5701 tactatcttt acaacaaagt tgacacaatt gttcagaagg tttcagaatt acaggagttg
     5761 attccggagg cttcgattgg atatgttcat ggtcgaatga gtgaagtcca gttggaaaat
     5821 actctattag actttattga gggacaatac gatatcttgg tgacgactac tattattgag
     5881 acaggggtgg acattccaaa tgctaatact ttatttattg aaaatgcgga ccatatgggc
     5941 ttgtcaacct tatatcagtt aagaggaaga gtcggtcgta gtaatcgtat tgcttatgct
     6001 tatctcatgt atcgtccaga aaaatcaatc agtgaagtct ctgaaaagag attagaagcg
     6061 attaaaggat ttacagaatt gggctctggc tttaagattg caatgcgaga tctttcgatt
     6121 cgtggagcag gaaatctttt aggaaaatcc cagtctggtt tcattgattc tgttggtttt
     6181 gaattgtatt cgcagttatt agaggaagct attgctaaac gaaacggtaa tgctaacgct
     6241 aacacaagaa ccaaagggaa tgctgagttg attttgcaaa ttgatgccta tcttcctgat
     6301 acttatattt ctgatcaacg acataagatt gaaatttaca agaaaattcg tcaaattgac
     6361 aaccgtgtca attatgaaga gttacaagag gagttgatag accgttttgg agaataccca
     6421 gatgtagtag cctatctttt agagattggt ttggtcaaat catacttgga caaggtcttt
     6481 gttcaacgtg tggaaagaaa agataataaa attacaattc aatttgaaaa agtcactcaa
     6541 cgactgtttt tagctcaaga ttattttaaa gctttatccg taacgaactt aaaagcaggc
     6601 atcgttgaga ataagggatt aatggagctt gtatttgatg tccaaaataa gaaagattat
     6661 gaaattttag aaggtttgct gatttttgga gaaagtttat tagagataaa agagtctaag
     6721 gaagaaaatt ccatttgata tttttcttct ataaaataga taaaaatggt acaataataa
     6781 attgaggtaa taaggatgag attagataaa tatttaaaag tatcgcgaat tatcaagcgt
     6841 cgtacagtcg caaaggaagt agcagataaa ggtagaatca aggttaatgg aatcttggcc
     6901 aaaagttcaa cggacttgaa agttaatgac caagttgaaa ttcgctttgg caataagttg
     6961 ctgcttgtaa aagtactaga gatgaaagat agtacaaaaa aagaagatgc agcaggaatg
     7021 tatgaaatta tcagtgaaac acgggtagaa gaaaatgtct aaaaatattg tacaattgaa
     7081 taattctttt attcaaaatg aataccaacg tcgtcgctac ctgatgaaag aacgacaaaa
     7141 acggaatcgt tttatgggag gggtattgat tttgattatg ctattattta tcttgccaac
     7201 ttttaattta gcgcagagtt atcagcaatt actccaaaga cgtcagcaat tagcagactt
     7261 gcaaactcag tatcaaactt tgagtgatga aaaggataag gagacagcat ttgctaccaa
     7321 gttgaaagat gaagattatg ctgctaaata tacacgagcg aagtactatt attctaagtc
     7381 gagggaaaaa gtttatacga ttcctgactt gcttcaaagg tgataaaatg gaaaatttat
     7441 tagacgtaat agagcaattt ttgagtttgt cagatgaaaa gctggaagaa ttggctgata
     7501 aaaatcaatt attgcgttta caaaaagaaa aggaaaggaa gaatgcgtaa attcttaatt
     7561 attttgttgc taccaagttt tttgaccatt tcaaaagtcg ttagcacaga aaaagaagtc
     7621 gtctatactt cgaaagaaat ttattacctt tcacaatctg actttggtat ttattttaga
     7681 gaaaaattaa gttctcccat ggcttatgga gaggttcctg tttatgcgaa tgaagattta
     7741 gtagtggaat ctgggaaatt gactcccaaa acaagttttc aaataaccga gtggcgctta
     7801 aataaacaag gaattccagt atttaagcta tcaaatcatc aatttatagc tgcggacaaa
     7861 cgatttttat atgatcaatc agaggtaact ccaacaataa aaaaagtatg gttagaatct
     7921 gactttaaac tgtacaatag tccttatgat ttaaaagaag tgaaatcatc cttatcagct
     7981 tattcgcaag tatcaatcga caagaccatg tttgtagaag gaagagaatt tctacatatt
     8041 gatcaggctg gatgggtagc taaagaatca acttctgaag aagataatcg gatgagtaaa
     8101 gttcaagaaa tgttatctga aaaatatcag aaagattctt tctctattta tgttaagcaa
     8161 ctgactactg gaaaagaagc tggtatcaat caagatgaaa agatgtatgc agccagcgtt
     8221 ttgaaactct cctatctcta ttatacgcaa gaaaaaataa atgagggtct ttatcagtta
     8281 gatacgactg taaaatacgt atctgcagtc aatgattttc caggttctta taaaccagag
     8341 ggaagtggta gtcttcctaa aaaagaagat aataaagaat attctttaaa ggatttaatt
     8401 acgaaagtat caaaagaatc tgataatgta gctcataatc tattgggata ttacatttca
     8461 aaccaatctg atgccacatt caaatccaaa atgtctgcca ttatgggaga tgattgggat
     8521 ccaaaagaaa aattgatttc ttctaagatg gccgggaagt ttatggaagc tatttataat
     8581 caaaatggat ttgtgctaga gtctttgact aaaacagatt ttgatagtca gcgaattgcc
     8641 aaaggtgttt ctgttaaagt agctcataaa attggagatg cggatgaatt taagcatgat
     8701 acgggtgttg tctatgcaga ttctccattt attctttcta ttttcactaa gaattctgat
     8761 tatgatacga tttctaagat agccaaggat gtttatgagg ttctaaaatg agggaaccag
     8821 attttttaaa tcattttctc aagaagggat atttcaaaaa gcatgctaag gcggttctag
     8881 ctctttctgg tggattagat tctatgtttc tatttaaggt attgtctact tatcaaaaag
     8941 agttagagat tgaattgatt ctggctcatg tgaatcataa gcagagaatt gaatcagatt
     9001 gggaagaaaa ggaattaagg aagttggctg ctgaagcaga gcttcctatt tatatcagca
     9061 atttttcagg agaattttca gaagcgcgtg cacgaaattt tcgttatgat ttttttcaag
     9121 aggttatgaa aaagacaggt gcgacagctt tagtcactgc ccaccatgct gatgatcagg
     9181 tggaaacgat tttgatgcgc ttgattcgag gaactcgctt gcgctatcta tcaggaatta
     9241 aggagaagca agtagtcgga gagatagaaa tcattcgtcc cttcttgcat tttcagaaaa
     9301 aagactttcc atcaattttt cactttgaag atacatcaaa tcaggagaat cattattttc
     9361 gaaatcgtat tcgaaactct tacttaccag aattggaaaa agaaaatcct cgatttaggg
     9421 atgcaatctt aggcattggc aatgaaattt tagattatga tttggcaata gctgaattat
     9481 ctaacaatat taatgtggaa gatttacagc agttattttc ttactctgag tctacacaaa
     9541 gagttttact tcaaacttat ctgaatcgtt ttccagattt gaatcttaca aaagctcagt
     9601 ttgctgaagt tcagcagatt ttaaaatcta aaagccagta tcgtcatccg attaaaaatg
     9661 gctatgaatt gataaaagag taccaacagt ttcagatttg taaaatcagt ccgcaggctg
     9721 atgaagagga agatgaattt gtgttacact atcaaaatca ggtagcttat caaggatatt
     9781 tattttcctt tggacttcca ttagaaggtg aatcaattca acaaatacct gtttcacgtg
     9841 aaacatccat acacattcgt catcgaaaaa caggagatgt tttgattcaa aatgggcata
     9901 gaaaaaaact cagacgttta tttattgatt tgaaaatccc tatggaaaag agaaactctg
     9961 ctcttattat tgagcaattt ggtgaaattg tctcaatttt gggaattgcg accaataatt
    10021 tgagtaaaaa aacgaaaaat gatataatga acactgtact ttatatagaa aaaatagata
    10081 ggtaaaaaat gttagaaaac gatattaaaa aagtcctcgt ttcacacgat gaaattacag
    10141 aagcagctaa aaaactaggt gctcaattaa ctaaagatta tgcaggaaaa aatccaatct
    10201 tagttgggat tttaaaagga tctattcctt ttatggctga attggtcaaa catattgata
    10261 cacatattga aatggacttc atgatggttt ctagctacca tggtggaaca gcaagtagtg
    10321 gtgttatcaa tattaaacaa gatgtgactc aagatatcaa aggaagacat gttctatttg
    10381 tagaagatat cattgataca ggtcaaactt tgaagaattt gcgagatatg tttaaagcaa
    10441 gagaagcagc ttctgttaaa attgcaacct tgttggataa accagaagga cgtgttgtag
    10501 aaattgaggc agactatacc tgctttacta tcccaaatga gtttgtagta ggttatggtt
    10561 tagactacaa agaaaattat cgtaatcttc cttatattgg agtattgaaa gaggaagtgt
    10621 attcaaatta gaaagaataa tctttaatga aaaaacaaaa taatggttta attaaaaatc
    10681 cttttctatg gttattattt atctttttcc ttgtgacagg attccagtat ttctattctg
    10741 ggaataactc aggaggaagt cagcaaatca actatactga gttggtacaa gaaattaccg
    10801 atggtaatgt aaaagaatta acttaccaac caaatggtag tgttatcgaa gtttctggtg
    10861 tctataaaaa tcctaaaaca agtaaagaag gaacaggtat tcagtttttc acgccatctg
    10921 ttactaaggt agagaaattt accagcacta ttcttcctgc cgatactacc gtatcagaat
    10981 tgcaaaaact tgctactgac cataaagcag aagtaactgt taagcatgaa agttcaagtg
    11041 gtatatggat taatctactc gtatccattg tgccatttgg aattctattc ttcttcctat
    11101 tctctatgat gggaaatatg ggaggaggca atggccgtaa tccaatgagt tttggacgta
    11161 gtaaggctaa agcagcaaat aaagaagata ttaaagtaag attttcagat gttgctggag
    11221 ctgaagaaga aaaacaagaa ctagttgaag ttgttgagtt cttaaaagat ccaaaacgat
    11281 tcacaaaact tggagcccgt attccagcag gtgttctttt ggagggacct ccggggacag
    11341 gtaaaacttt gcttgctaag gcagtcgctg gagaagcagg tgttccattc tttagtatct
    11401 caggttctga ctttgtagaa atgtttgtcg gagttggagc tagtcgtgtt cgctctcttt
    11461 ttgaggatgc caaaaaagca gcaccagcta tcatctttat cgatgaaatt gatgctgttg
    11521 gacgtcaacg tggagtcggt ctcggcggag gtaatgacga acgtgaacaa accttgaacc
    11581 aacttttgat tgagatggat ggttttgagg gaaatgaagg gattatcgtc atcgctgcga
    11641 caaaccgttc agatgtactt gatcctgccc ttttgcgtcc aggacgtttt gatagaaaag
    11701 tattggttgg ccgtcctgat gttaaaggtc gtgaagcaat cttgaaagtt cacgctaaga
    11761 acaagccttt agcagaagat gttgatttga aattagtggc tcaacaaact ccaggctttg
    11821 ttggtgctga tttagagaat gtcttgaatg aagcagcttt agttgctgct cgtcgcaata
    11881 aatcgataat tgatgcttca gatattgatg aagcagaaga tagagttatt gctggacctt
    11941 ctaagaaaga taagacagtt tcacaaaaag aacgagaatt ggttgcttac catgaggcag
    12001 gacataccat tgttggtcta gtcttgtcga atgctcgcgt tgtccataag gttacaattg
    12061 taccacgcgg ccgtgcaggc ggatacatga ttgcacttcc taaagaggat caaatgcttc
    12121 tatctaaaga agatatgaaa gagcaattgg ctggcttaat gggtggacgt gtagctgaag
    12181 aaattatctt taatgtccaa actacaggag cttcaaacga ctttgaacaa gcgacacaaa
    12241 tggcacgtgc aatggttaca gagtacggta tgagtgaaaa acttggccca gtacaatatg
    12301 aaggaaacca tgctatgttt ggtgcacaga atcctcaaaa atcaatttca gaacaaacag
    12361 cttatgaaat tgatgaagag gttcgttcat tattaaatga ggcacgaaat aaagctgctg
    12421 aaattattca gtcaaatcgt gaaactcaca agttaattgc agaagcatta ttgaaatacg
    12481 aaacattgga tagtacacaa attaaagctc tttacgaaac aggaaagatg cctgaagcag
    12541 tagaagagga atctcatgca ctatcctatg atgaagtaaa gtcaaaaatg aatgacgaaa
//
LOCUS       DAVKJJ010000052        12562 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000052 DAVKJJ010000000
VERSION     DAVKJJ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12562
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.052"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(260..1096)
                     /locus_tag="VW144_001658"
     CDS             complement(260..1096)
                     /locus_tag="VW144_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9601075.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPELLKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKNGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(1109..1738)
                     /locus_tag="VW144_001659"
     CDS             complement(1109..1738)
                     /locus_tag="VW144_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9601076.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSILDNLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(1748..2389)
                     /locus_tag="VW144_001660"
     CDS             complement(1748..2389)
                     /locus_tag="VW144_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9601077.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(2535..3764)
                     /locus_tag="VW144_001661"
     CDS             complement(2535..3764)
                     /locus_tag="VW144_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU9601078.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLAAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(3754..4920)
                     /locus_tag="VW144_001662"
     CDS             complement(3754..4920)
                     /locus_tag="VW144_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9601079.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            5064..6077
                     /locus_tag="VW144_001663"
     CDS             5064..6077
                     /locus_tag="VW144_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEU9601080.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(6122..6541)
                     /locus_tag="VW144_001664"
     CDS             complement(6122..6541)
                     /locus_tag="VW144_001664"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU9601081.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(6552..7958)
                     /gene="atpD"
                     /locus_tag="VW144_001665"
     CDS             complement(6552..7958)
                     /gene="atpD"
                     /locus_tag="VW144_001665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU9601082.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(8044..8922)
                     /locus_tag="VW144_001666"
     CDS             complement(8044..8922)
                     /locus_tag="VW144_001666"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEU9601083.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(8938..10443)
                     /locus_tag="VW144_001667"
     CDS             complement(8938..10443)
                     /locus_tag="VW144_001667"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU9601084.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(10458..10994)
                     /locus_tag="VW144_001668"
     CDS             complement(10458..10994)
                     /locus_tag="VW144_001668"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEU9601085.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(10994..11488)
                     /gene="atpF"
                     /locus_tag="VW144_001669"
     CDS             complement(10994..11488)
                     /gene="atpF"
                     /locus_tag="VW144_001669"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU9601086.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(11502..12218)
                     /gene="atpB"
                     /locus_tag="VW144_001670"
     CDS             complement(11502..12218)
                     /gene="atpB"
                     /locus_tag="VW144_001670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU9601087.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNILEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(12253..12453)
                     /locus_tag="VW144_001671"
     CDS             complement(12253..12453)
                     /locus_tag="VW144_001671"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEU9601088.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
BASE COUNT         3858 a         2790 c         2238 g         3676 t
ORIGIN      
        1 taaaatcaca aaaacgcata atgtaaggtg caaaaacctt gatattatgc gttttattgt
       61 tcctaaaaca gttacatcag tatttcatta cagaacattt taattttgga agtcaaatca
      121 atttttaaca atgttttaga aatcacagtg ggctattcca tattcaatta tccagattca
      181 atcgctatta tcgttttcct agattacaaa aaaaactacg ttttgctaat caaaacgtag
      241 tctcagccac tattttatct tactgtcctt cttttacttc tttggttgct acatcttctc
      301 caaaccattt ttggctgatt tcttggaact tgccgttctt gtaaagacta gaaaaagctt
      361 catttatctt cttaaccaag gttgtatctt ccttacgggc tccaaccgca aaagcttctg
      421 tttctagtcc aactgtaaag acattataat cgtttaaaac accttctgct tctaaataat
      481 agtttgcata gacacggtca atcaatagac catcaattcg atcgtttttc aaatcaatca
      541 aggcttcatt aaaggtttgg tattgattcg cttccttatt agcgacaata ttcttcaaaa
      601 gttctggatt tgcttcaaag tccgcataac cagatgaacc agcttgagct cctaatgtct
      661 ttccagtcat atcctttgca gtcgtgatac cagatgattt cttcgtaacc aatacctgct
      721 cattcttcat atatgagtta ctgaaagcca ccttttcacg gcgttcgtct gtagcggaat
      781 agccattcca aatcagatca atcgttcctt ttgtcaattc agcttctttc aaatcccaat
      841 caatcggttg ccaatttacc gtgattccgt atttttcaaa aacagctgta gctaaatcaa
      901 tatcaaatcc tgcataagaa ccatctttct gagcaaatcc cattggaaca aaagtactat
      961 caaatccaat agtaatagac ttgttagact ggtactttga ccaattatct ccactagttt
     1021 cgctagaatt tttcccacaa gctactaaga acaaagcagt catcagactg actaatacaa
     1081 gcatccattt tttcatccat ttttcctcct atttaggttc tactttcaat aatacatctg
     1141 cgatattttc agcaaactgc aaatcatggg taaccacaat ctgggtcatc ccaagttccc
     1201 tattttgcaa gattaatttt tccacttcca agcgtaattc tggatccaga gcagaagttg
     1261 gttcatcgta gccaatgatt tctgggtcaa tcatcatagc acgcgccaaa gccacccgct
     1321 gcttttgccc accagatagt gagaaaggat aggcctctgc gtgtcctcct agccctaact
     1381 gttccaagag tccactcgcc ttcttctcag cctcttcctg cttcattccc atggtcttca
     1441 caggcgataa ggtcaaattg tccagaattg atagatgagg aaatagttga aaatcttgga
     1501 agacaaatcc cagtaggttg cgcttctgca attcatccag ctctaaaggt tgtccattat
     1561 aaaagatttg ccctgaatca atggtttcaa gacccgcaag catacgtaaa agagtcgtct
     1621 tacctccacc agaaggtcca acgatagcca ggatttgctt ttcaggaata cttagactga
     1681 aattagacag gatttgtttg tctccaaaga ctttattgat atttcgtaat tctaacatgg
     1741 cagcctccta tctataataa ctatacttct tctcaacttt tttggaaata attgtcacaa
     1801 tcccaatcaa aatcaaataa atggctcctg ccaagaacat aggaactaga ctagcatcgc
     1861 ggttagcagc tgttcgacta gccaagataa ggtctgaaat tccgagagca tagaccaaag
     1921 aagtatcctt gaccaaactc ataacttcat taaagacact aggaagaacg atcttggtca
     1981 cttggggcaa gataatatag cgcactctgt caaaagggct aaacttcaag accttggcag
     2041 cctcatactg tcctcttgga atagtgtcaa ttcccccacg gaaaatttct gcaaagtaag
     2101 ctgcatagtt gagaacaaag gcaataatag ctgcaggaag gcggtctaaa cgaatcccaa
     2161 tacttgggag cacataatag ataaaaatca gttgcaagag taagggggtt cctcgcataa
     2221 cccagatgta aatgttaatc agataatgga ggggcttcca atggacttgc aaggcaaagg
     2281 caatcaaaac gcccaaggga atcgaaaata gcaagaccag tgcaaagacc tgtacagtcg
     2341 tgctcgcacc attcagtaaa ctcggtaata tctcaaataa ataagacata ctgcacctcc
     2401 taaaagtaat tgttcctatt atagcatgaa taaacaagat taccaagtat ttttgtaaaa
     2461 agattctacc atatacaaat aaaaaccgta gctatcaaaa gagataaact acagttctat
     2521 atgtttaaac attcttacaa tctctcaaac agttcttgca tttgcacatc atctggaacc
     2581 agttttaagt aagtgtgagc atggactttt gcttcttcaa aatgtcccaa ttcacgcaag
     2641 agatagatat agtgttccag aaattctgga ttgtccttca aatctcctgt caactcttga
     2701 taatgctcat aagcagtatc caaatcgtcc atttcttgat aagaacgagc aatcatccac
     2761 ttggtcaaaa gattttctgg ctcctcactc tgcaagtcta gaatatcctc ataacgctcc
     2821 tgctccagat aaatagtggc taaacgaagc aagatttctt ctgtatcctc agcgtcttct
     2881 tttgcagcaa gaagataatt ttctgcacca ctagcatcat gcaattcata agaaaattgt
     2941 gaagcagcta gcaagaggcg agtttcaaag ggatttttct ctaatccttg cttagcgata
     3001 cgcagggctt cttgaacttg atgttcctta tgtaaagcct gactgtaccc atactcatag
     3061 ccttcaaagt caggagaaat ggtatcaagc tgcttaaagt agagggtggc tttttgatat
     3121 tcttcttgat caaaataaag actggccaac tcaaaagctg ttaagtcatc gtattctaac
     3181 tccagggctt tttctaaaaa ctcagtagcc gtttcaaatt tccctaactg agcataggca
     3241 aagccaattc gttgataggt ggaaatgccc gtttgctcat aaatcgagcg attatctaac
     3301 tgggcatagg cttgaatagc cgcttggtaa ttttccaact cactatccaa ctctgccaaa
     3361 cccaatatca agagagaatc ctctgagtag gtcaaggcct ccaataattt ctcacgtgcc
     3421 acatctgtca aaccttccag ctggtaaagg tctgccttca gagccaaaga cgagacatac
     3481 cagtcactgt cagcttggat ttcctcaaga taggtaaagg cttcttctat ttgaccatcc
     3541 tcgctagcaa ttgcagctag attaagatga acctctggaa aatcctctac aattttcagg
     3601 taaatttcct tggcctgagg atagaaacca atcccttcaa gataagttgc caactcatac
     3661 agaagatcac ttgaatcatt ttctaaagct ttggcgaaat aatgctcagc cttagttaaa
     3721 tcttgctcct ccaaagcctg taacatctgt tgactattgt tcactcttga cttcctcacc
     3781 ctctaattca tatagaccac tgactacctt ataccattcg aaaatggctg aaatgacaac
     3841 cttagcagag gcataaaccg gaataccaag taaaactccc cagataccaa acatagatcc
     3901 tgaagttaac aaaacaaaga gaacattaat agggtggatg tttaattgac ttcccaaaat
     3961 caatggagag acaaaacggc cttcaatagt ttgttctacg ataaagacaa tcactacttt
     4021 caaaagcatg actggaccag caatcaaacc caatactaga gcaggaagca tggctagaaa
     4081 gctaccaaga taagggacca gatttaaaat accagcagta acccccagcg taaccgcata
     4141 gcgtagacca ataatcttga agaagatgat aaacattact gctacaataa tagccactgt
     4201 cacttgccct cgaacatagt tggacaactg ttgattcaca tctgataaaa cttgtccaac
     4261 aggttccttc aattttcttg gaatgaattg ggtcaaatag ttacgcaagc ctttcccatc
     4321 acgcaagaga taaaagagca tgaaaggaac gataatcaag gcaacaatca cttgagaagc
     4381 tccgctaata aaggcactca cccagttgac tgcctgagat gaaaccttac ttgccaaaac
     4441 tgtagcctgg ctagaaaaat tggtcaaaac ttgctctaat tgaggtctga aatcatctgg
     4501 caggtgctgg gctaccaatc cattaacaat cctatctata tcttctaagt aaacaggaac
     4561 gtttcttgca aaggtcaaaa cctgacgttg cagatttgga atggcgactg ccaagcccca
     4621 aatgataaag agagcgatga taacaaagac aatagtgata gctataacac gattaacctt
     4681 atgcttctcc atccaatcaa caataggatt caacaaataa tataacaaac cagacaaaat
     4741 gactggcaac atcacaactg ctaaaaagtc taaaacaggt gaaaatagaa aactaatctt
     4801 acttaaaata aaaagattca gtcccaataa taaggttact aaaaataccg taattgcctt
     4861 gttatctaaa aaccacttga aaaaccaaga taggctaaaa tgtttctctt tttgctccat
     4921 aaatacttcc tttctattgt tctctcatta taccattttt gaaacaaaat aactcttttt
     4981 taaagtgctt acatctaatt ggatatcttt aatttacttg tttttgtgat ataatagtct
     5041 tatctttaca cagaggtata gtaatgaaag aaactgttta ttttggaact tatacacgtc
     5101 gtacttctca agggatttac aaggcagact ttgatacaga aactggtcag ctttcaaatc
     5161 tagaactttt tgcagctgag ccaagtccaa cctaccttgc ctttgaccag caccaacatt
     5221 tatacactgt tggtagccaa gacgataagg ggggaattgc agcctatcaa actgacggga
     5281 ctgtgttaaa tcatgttgtt gaagaaggag ctccccactg ttatgttgct gtcgatgaaa
     5341 agcgtgattt ggtttacgca gccaactatc acaagggaca agtccttgtt tataaacgcc
     5401 aggaagatgg tagtcttcta cttagtgata tggatcaaca cagtggccaa ggtccacatg
     5461 aaaatcaagc ttccccccat gttcactata cagatttaac acctgaccac tatctagtga
     5521 cctgcgactt gggaactgac caagtcatca cctatgacct cgatcaagaa ggaaaattat
     5581 ctaagctcta tacctatcac agcaagccag gagcaggctc acgccatatc attttccata
     5641 accactataa aatcgcttat ctcatttgtg aactcaatag tactatcgaa gttttaatct
     5701 acgatggcgt tggcgaattt gaacgtatgc aggtcatttc aactttaccg gaagcttacg
     5761 aaggctttaa tggaaccgct gctattcatc tctctaaaga cggtaaatac ctctacgctt
     5821 ctaaccgtgg ccatgattct atcgcagtat ataccatcct tgcggacggt agcttagagt
     5881 tattagaaat cgttccaacg catggtcaga ctccacgtga ttttgatttg acacccgacc
     5941 aaaaatttct cattgttgtc catcaagact ctgacaatgc aactgtcttt aaacgtaatt
     6001 gtgacaatgg tcgtctagca gaactctcca acgacttcca tgttcccgaa gcagtctgca
     6061 tccgttttgc tccttaaaac accataaaaa atgaacttgg tattttcaag ttcatttttt
     6121 cttatagtct atttccgaca ttaatacggt taatagcacg ttgcaaagca atcttagcac
     6181 gacgttcttg gtcaatcaaa tgtttgtctt gtgcttcttc aattgcacgt tctgcacgaa
     6241 gtttggcacg ttctgcacga ctgatatcga tatcacgagc acgttctgca gagtcagcga
     6301 cgattgtgat catatcattg gcaatttcaa taacaccacc gtttactgca atccagttca
     6361 cgtgatcttt atcatcgata cgttttacct ttacttcatc aactgctaaa accgcaatca
     6421 tattttcatg tcgtggcaag atccccatct caccatccag agttcgaacc gatacatagc
     6481 tggcatggtg atcatagacg agaccatctg gtgtcacgat ctggacagtt aactgagcca
     6541 tagttcacct cttaaaatcc cattttttca gcttttgcaa tcacatcttc gatagaacct
     6601 acaccacgga aggcatcttc tggcaagtgg tcgtatttac catcaaggat ttccttaaag
     6661 ccacgtacag tttcagcaac tggaacataa gaacctggct gaccagtaaa ttgttccgca
     6721 acattgaagt tttgtgacaa gaagaactgg atacgacggg cgcgagcaac caaggtcttt
     6781 tcttcatcag aaagttcatc cataccaagg atagcaatga tatcttgcaa ttcatggtaa
     6841 cgttgaagga cacgttttac ttcagcagca actgcatagt gctcttctcc aacgatttca
     6901 ggtgccaagg cacgtgagct tgaagcaagt gggtcaacgg ctgggtagat acccaattgt
     6961 accaacttac gttccaagtt tgttgttgaa tccaagtgag cgaaggctgt tgctggcgct
     7021 gggtcagtat agtcatccgc tggcacatag atagcctgga tagaggttac agaacccttc
     7081 ttggttgatg tgatacgttc ttgcaattga cccatttccg tagcaagtgt tggttggtaa
     7141 ccaacggctg atggcatacg acccaaaagg gcagatactt ctgaaccagc ctgagtgaaa
     7201 cggaagatat tatcgataaa gagaagcacg tcttggcctt ccacatcacg gaagtattca
     7261 gcgattgtca aaccagtaag ggcaacacgc atacgtgctc ctggtggctc attcatctga
     7321 ccaaagacca tggctgtttt ctcgataacg cctgattctt tcatttccca gtaaaggtca
     7381 ttcccctcac gagtacgttc cccaacacca gtaaatactg aaataccacc gtgctcttgg
     7441 gcaatgttgt gaatcaattc ttggattaag acggttttac caactccggc accaccgaaa
     7501 agtccaactt taccaccttt aaggtaaggg gcaagaaggt caataacctt gatccctgtt
     7561 tcaaggattt cagaagaggt agacaactca tcaaaagttg gagctttttt atgaattggc
     7621 tgacgctctg cgtcttctgt aaaaggagct tccaaatcaa tggtatctcc caaaacgttg
     7681 aagacacgtc ccaaagtttc cttacctact ggtacagaga ttggacgacc tgtgtccaat
     7741 acttccattc cacgagtcaa cccatctgtt gattccatgg cgatagtacg aaccatacca
     7801 tctcctaact ccaaggctac ttcaaggacg atttttgttt ttctttcgtc atttttgtag
     7861 acgacaagtg cattgttaat ctcaggaagt ttttcccctg ctgcaaacaa aacgtctaca
     7921 acgggaccga taacctgagc aattttacct gaactcatct ccttctccta ttctatataa
     7981 gatactgtcg gttcctagct caactaggtc ctaagttcat tttcatacga gctggactag
     8041 agcctattct aaggcactag cacctgctac gatttctgta atttcttgtg taatcgccgc
     8101 ctgtctggca cggttatact gaattgtcaa atcattgatg actttcttag cattatctgt
     8161 cgctgtttgc atggctgtca tacccgcagc attctcagct gtcttggcat cgataatggc
     8221 accgtaaatc atactttctg caaactgagg caacaactgc tccagaattt cttctcggct
     8281 ggtttccaat tcaaaagtca agctgtactc ttcatccgct tcatttggat ccaagtcaac
     8341 aatcggaagc atttgttcca cacgcatttg actggttagc gtattgacat ggtggttgta
     8401 gcaaacataa agctcatcaa agagttcatt ttggtacatt tcaacagttt ttgaaataat
     8461 cttacgaact tgatcaaagc taggttggtc tgccaagcca cgtaattcat aaagtggttg
     8521 aataccgcga gccttaaaga aatcagctcc catcccaccg atacagatca tttcaaaacc
     8581 tttaccgtct gggtggtatt cttctttcaa ctccataaca gctttcaaaa tagaggaatt
     8641 ataacctcca accaaaccgc ggtctgaagt gataacgata tagcctgtct tcttcacaga
     8701 acggctaatc aacatcggat tagttgaagc accagctcca ttaccatgaa ggatatctgt
     8761 caaaagttta cgcactttct gagcgtaaac ttggaagttg cgagcagctt cttcagagcg
     8821 acctaactta gcagccgata ccatttgcat ggcattagtg atttgactcg tattttttgt
     8881 tgaggcgatt tttgttttaa tatcatttag agacactgcc atctgacacc tctattctta
     8941 ttggaagcta gattgattga gaaactctgt aatcgcagca tccaagactg cttcttctgg
     9001 caagtctttt gtatcacgaa tggtttccaa aatctctgga tgttgagcat caaagaaggc
     9061 atggaactct tcctcgaaac gaacaatatc atctactgga acagtatcca agaaaccatg
     9121 tgtcaaagca taaagaatgg ttacttgttt ctcaacaggt aatggtttgt gaacaggttg
     9181 tttcaagacc tcaacggtac gacgtccacg gttcaactta gcctgtgttg ctgcgtccaa
     9241 gtcagaacca aacttagtaa aggcttccaa ctcacggtat gaagcaaggt cgatacgaag
     9301 tgtaccagca accttcttca tggctttgat ttgtgcagaa ccacctacac gagatacaga
     9361 tgaacccgca tcgatggctg gacgaatacc tgcattgaag aggccatcgc caaggaagat
     9421 ttgtccatca gtgatagaaa tcacgttggt tgcgatatag gctgagatat ctcctgcttg
     9481 tgtctcgata aatggtaggg ctgtaattga tccaccacca agttcatcag aaactttagc
     9541 tgagcgctca agcaaacggc tgtggagata gaaaacatcc cctgggaagg cttcacgacc
     9601 tggaggacga cgaagcaaga gcgatagttc acgataagct accgcttgtt ttgatagatc
     9661 atcgtataca atcaaaacat gcttaccttg atacataaat tcttccgcca tagcaacccc
     9721 agtataagga gctaggaaga gcaatggaga tggttgtgaa gcagaggctg tcacaacgat
     9781 tgtgtagtcc aaggcaccgt actgacgaag tgtttctact tgcgtacgaa ctgttgattc
     9841 tttttgtcca atcgcgacgt agatacagat catatcttga tctttttggt tcaagattgt
     9901 atcaatcgca atggttgttt tccctgtctg acggtcaccg ataatcaact cacgttgacc
     9961 acgaccaatc ggtacaaggg cgtcaatagc tttcaaacca gtttgcaatg gttctgaaac
    10021 agacttacgt tgcataacac caggagctgg tgcttctact ggacgagttt tatcagtgtg
    10081 gatttctcca agaccgtcaa ctggacgacc aagcggatcc acaacacgac caatcagact
    10141 ttcacctaca gggacttcca tgattttccc tgtacggcgg attgtatcgc cttcacggat
    10201 atctgtaaag tcacctagga tgataatacc aacgtctgtt gactccaagt tttgagccat
    10261 accataagag ccgttttcaa aattcaacaa ctctccactc atgacatttt caaggccatg
    10321 agcacgcgcg ataccgtccc cgatataggt tacaacacct gtttcagtca catcaaaatt
    10381 gggtttgaaa ttttcaattt gttgcttaat taaagcgctg atttcttgtg cgttaattgc
    10441 caaaagaaca ccactttcta tttcaaattt tctttaacaa ctttaagttg ttgtttaata
    10501 ctcacatcaa ttgtcttgtg attggcaaaa atgacaaaac caccaatgag actttcatcg
    10561 atttgttctt ttacactcct tactttcaga gacatttttt tctcaatcaa agggagcaaa
    10621 cgagtcttct gttcatcagt tagaggatga gcagacgtaa tcgtcacttc aaatcgattt
    10681 gtttcttttt caagtcggtt caagcaatct acaagcacat cataaaaaag atttgctctg
    10741 tgattgtagg ccagaacctg gataaagttt tgtaataaag gcgacacaga atcttggaaa
    10801 aaagcaattg ttttttcctt atccgactcg tctactgcca cttgttttaa aaaagaaggc
    10861 agacctgttt tttcaacaac ttgcttgatt tgagtcaagt ctgaaaagat acggtcttct
    10921 tctccttttt caagtaccaa ttggacaaaa ggcatgctgt atttttcaat tacctttact
    10981 gttttcttgt ccattaagct tctcctagct gatcgatata ctgatcaatg agtgctttat
    11041 gggcatgact gtcaaggttt tgtgagatga ttttaccagc taagctgatg gtcaaatctg
    11101 cgacctcacc cttaacactc tgtaaagctt ctactttatt ttgagcaatt tcttgattgg
    11161 ctttttcttt taagtgtcct gcttctagtt tagcatctgc taagatatta gccttacttt
    11221 gctcagctgt ttcctttgca ttttcaatga ttgtcttagc ttctttacgg ctaccagcca
    11281 attcatcttc gcgtttttga gccaatactt ctgctttttg acgggcttct tcagctctat
    11341 caatatctga agcaattttt tcagctcttt cttcgaaaat gcctgtaata ttagaccatg
    11401 caaatttttt aatcaagact agcaaaagaa taaaagagcc agtgattaaa ataaaattac
    11461 caattaattc acctactgtt acgtgcatca gttactcctt tctattcctc ttcaatatta
    11521 atcttattcc ctaaatacac agatgtcaaa agagtaaaaa cataagcttg gatgcaggaa
    11581 ataaagacag aaaatgcagt ccaagccaaa ttagctccaa aggctactgg ataccaataa
    11641 atagcttggt gggaaagaag aagtaacaaa ctcgtcatga cctctcctgc aaagatattc
    11701 ccaaaaatcc gcaaagccaa agataagaag tttgtaaatt cttccaagat attcatcggt
    11761 atgacaaaaa caggactcat aaaagatttt atactttttt taaatccacg acgacgaacg
    11821 ctttctatat gtgtcaacaa aatgacaaga aaagataagg ttaagtcata ctgtaaatta
    11881 gcggttggcg aactccacca gttagtccca tcgatcgttt gaagctttgt cattaagcca
    11941 aggttattgg caatcaccat gaaaaggaat aaacaaagga aaaagagtga gtaatctttc
    12001 atgtagcgcg aaccaatgtt aggttctgta aatccaataa caaagtcata gacatactca
    12061 agtatatttt gctttccttt gggtttcaag gtcatattgc ggcttgccca ataaataaag
    12121 acaaaaataa ctcccacaat caacaaagtc atggctaaca tagtcagatt gaagataaca
    12181 ggaccaatag agatgattgg attaatactt tcttccatct aaaatcctcc cttttcttct
    12241 cgttccatgt atttatttga taataaatga gaagacaaga gttacaaaga aagttccttc
    12301 aataaaggca acacctaaaa acatcaaact acgaaactca gaaagcatat ctggttggcg
    12361 tgctactgat ttaaacagtc cattcatcaa taaaccttca ccgacagata cgcccataca
    12421 ggcaatacat aagcctaaaa atgttaaatt cataacaaat tctcctttaa aataaaattt
    12481 acttacttag tgtagtccta aaatttaaat ttgtcaactt ttaaccgcta ttggaagcgt
    12541 ttaataaaaa ttatttccat tt
//
LOCUS       DAVKJJ010000053        12381 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000053 DAVKJJ010000000
VERSION     DAVKJJ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12381
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.053"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            111..275
                     /locus_tag="VW144_001672"
     CDS             111..275
                     /locus_tag="VW144_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9601089.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            359..925
                     /gene="amaP"
                     /locus_tag="VW144_001673"
     CDS             359..925
                     /gene="amaP"
                     /locus_tag="VW144_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEU9601090.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            937..1107
                     /locus_tag="VW144_001674"
     CDS             937..1107
                     /locus_tag="VW144_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEU9601091.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1146..1754
                     /locus_tag="VW144_001675"
     CDS             1146..1754
                     /locus_tag="VW144_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9601092.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1785..1988
                     /locus_tag="VW144_001676"
     CDS             1785..1988
                     /locus_tag="VW144_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9601093.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2081..2359)
                     /locus_tag="VW144_001677"
                     /pseudo
     CDS             complement(2081..2359)
                     /locus_tag="VW144_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2569..2844
                     /locus_tag="VW144_001678"
     CDS             2569..2844
                     /locus_tag="VW144_001678"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9601094.1"
                     /translation="MKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSKWKDI
                     SNHHDSESFFANLGCSRQRRLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(3122..3892)
                     /locus_tag="VW144_001679"
     CDS             complement(3122..3892)
                     /locus_tag="VW144_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU9601095.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLYQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(3907..4755)
                     /locus_tag="VW144_001680"
     CDS             complement(3907..4755)
                     /locus_tag="VW144_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEU9601096.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVSNACWKH
                     PYGENNNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(4752..6227)
                     /locus_tag="VW144_001681"
     CDS             complement(4752..6227)
                     /locus_tag="VW144_001681"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEU9601097.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHTLGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(6551..7906)
                     /locus_tag="VW144_001682"
     CDS             complement(6551..7906)
                     /locus_tag="VW144_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9601098.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNYLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            complement(7916..8233)
                     /locus_tag="VW144_001683"
     CDS             complement(7916..8233)
                     /locus_tag="VW144_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9601099.1"
                     /translation="MNESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTKEAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(8250..8567)
                     /locus_tag="VW144_001684"
     CDS             complement(8250..8567)
                     /locus_tag="VW144_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9601100.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(8582..9478)
                     /locus_tag="VW144_001685"
     CDS             complement(8582..9478)
                     /locus_tag="VW144_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEU9601101.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLKRIEELGFDFVCLTGNPGTGVSNREIIKTVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(9481..10743)
                     /locus_tag="VW144_001686"
     CDS             complement(9481..10743)
                     /locus_tag="VW144_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9601102.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFITGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQGALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
     gene            11023..12201
                     /locus_tag="VW144_001687"
     CDS             11023..12201
                     /locus_tag="VW144_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU9601103.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT         4301 a         2454 c         1895 g         3731 t
ORIGIN      
        1 ttgatttttt gatattaaaa taaaaaagga gggtaggcag tgttgtgatc aattattgct
       61 ggaggtctta ttggtctctt ggcaggtaaa atcactaaaa aagtagttct atgggaatca
      121 tcgcaaatgt attcgctggt ttagtcgggg catatgcagg acaatctctt ttaggtagtt
      181 ggggtccatc aatcgctgga atggctttgc tcccatctat tgtaggtgca gcgattgtga
      241 ttactgtagt gtcattcttt acaggtagaa agtaaacttt tcgccagtaa agttagcaaa
      301 ctatttttaa atcaatgacg ggaaaaatag tttaaatgtt aaatcgaaag gattgtatat
      361 gtcaaaagca aagaaaatat gtttcattat tttctgtatt ttaatcttga caattttcct
      421 tcctgttttg atagattatc atcaagttag tgatctaggt attcatctac tgagctggag
      481 acagaactcc gtagttgaat tctatcttgc tagatatgtc ttttggggga cagtggttct
      541 atcaacttta gttttattat ccattttagt tgtgatgttt tatcctaaac gttacttgga
      601 aatccaactt gaaactaaaa acgatacatt aaaattaaag aattcggcaa tcgaaggttt
      661 tgttagaagt ttggtgagtg atcatagatt gatcaagaac ccaactgttc atgtaaattt
      721 acgaaaaaat aaatgtttcg ttcatgtaga aggtaaaatt cttccttcag acaacatcgc
      781 tgacagatgc caaataattc aaaatgaaat aactaatgga ttgaagcagt tttttggtat
      841 tgagcgtcaa gtaaaacttg aagttgcagt aaaaaattac caaccaaaac ctcaaaacaa
      901 aaagactgtt agtcgtgtga agtaaggaag taaaaaatgg aatggcttaa acaatatcga
      961 tatccaatta tcgctggtct cataggcgta tttctggctt gtttgattgt ctcctttggc
     1021 ttcttcaaaa caatatttgt attgatttta ggagcactgg gagttgcagc tggattatat
     1081 atcgaaaaaa actatataga taaataaaaa aataaaaatt actaatttaa ttaaaggagt
     1141 ttcatatgtc aaacgaaaaa aacacaaaca ctaacgtaga aaagaaagat gctactgttg
     1201 tagctcacga aatcaaaggg gaacttactt acgaagataa agttatccaa aaaatcattg
     1261 gtctttcact agaaaacgtt tcaggtcttt tgggaatcga tggtggtttc ttctcaaatc
     1321 ttaaagaaaa aatcgttaac agcgatgacg taacaagtgg tgttaacgta gaagttggta
     1381 aaacacaagt tgcagttgac ttaaacgtta ttgttgagta ccaaaaaaat gttccagctt
     1441 tatattcaga aatcagagaa atcgtatctt cagaagttgc taaaatgact gacttggaaa
     1501 ttgttgaaat caacgtaaac gttgtcgaca tcaaaactaa agaacagcat gaagcagact
     1561 cagtaagcct tcaagatcgc gtatctgacg ttgctgaatc aacaggagaa ttcacttcag
     1621 aacaattcga aaaagctaaa tctggtcttg gatctggttt ctcaactgtt caagaaaaag
     1681 ttagcgaagg tgtagaagct gttaaaggtg cagcaaatgg tgtagtatct cacgaaaaca
     1741 ctcgtgtaaa ctaagataaa ataaatataa caggagaaat tatcatgtca gtagaagaaa
     1801 aattaaatca agctaaaggt tctattaaag aaggtgttgg gaaagccatc ggtgatgaaa
     1861 aaatggaaaa agaaggagca gctgaaaaag ttgtttctaa agtaaaagaa gttgccgaag
     1921 acgctaaaga cgctgtagaa ggtgctgtag aaggtgttaa aaacatgttg agtggcgacg
     1981 ataaataagg ttattggtaa cattagtcaa aagagtctga gtcaagatga ttctcagaaa
     2041 acaaaaagct agagattccc aattgcggaa ctctagcttt ttaattttgc ctctttctct
     2101 tattatattt cagcaggttg ttggccatga gtatgaatcc catgtcaatt ctcacttgac
     2161 gcttacctct cagatgacat ctcttataac ccaaacaaac ctttatctgc ccaaagacag
     2221 atttcatatc aatcttacgt ttagcgaaaa tttgtctacc cttggaagat aaaagtgcct
     2281 gatattcttt agtttttaaa cactggtaac gttcattcat atacagtccc ttttgagggg
     2341 ctgattcagg ttcataatcg cagtcaacat tgatttcaag gctgtttgct ttctatctcc
     2401 cctttttcat gatatatttt cgtacaggtt cttcaatagt ttctatagta aaatgaacca
     2461 aaaatagtac acgttgcggt ataatgttct tgtggcatat tcattagatt ttcgtaaaca
     2521 gttctctcct atttgtgagc gaacaagtat agtcaattga ttgtcagttt gaagaccaag
     2581 agtcgtaagg caaaggatat ggcagaatcc atccaagggt ggctggcaca atttctagtg
     2641 aacctcttta aatctattac gtttgactgt ggaaaagagt tttcaaaatg gaaagatatt
     2701 tccaatcatc acgatagtga gagctttttt gcgaatctag gatgttcaag acagcggcgt
     2761 ctcaacgaac attcaaatag acttttaagg tgtcatgact tgccaaagca gacagatttc
     2821 aacgaagtca gtcaagaatt ttaggctgga caaaaactca atttcaggag aaaatgaaat
     2881 aaatattccc acaataaaac gcatagtatc aaagtttttc caacacctga tattatgcat
     2941 tttttaattt taaagacttt ttcccagcct caagaaatca catcccacct aaatcctttg
     3001 gataccaaat acctcatcag gtattttctg agttggtaag actgtctagc ttaatttgac
     3061 aaatggaggg cgttaaatcc agtttttaca ggaagtctat ttaaatattt tcacactgac
     3121 cttacaaaat atgaaataaa ttatataaag tcattcccat gacaaagaat aacatccctt
     3181 taaaacagta tcttacccaa ttttcaggta agacttttga tacaaccgtc cccaataccc
     3241 ctccgcatat agaagcaaga aaaataactg gaacaggtgc taaatgatac tgatttaaac
     3301 ctgtaaccac aacaattgta gccaaatgag acagctggga aaagaaaata attgcaagag
     3361 aatacatcgt tgcttctttt atcgaaatag aaaaaaacac catcaataaa gatacattga
     3421 gcggaccacc accaatccct aaaaaactag aaattaagcc caatagaaga ccacaaatca
     3481 tatagaatcc tatcttatca aattgataag tttttttaaa atctgttaaa acaaatgata
     3541 caagtaacat cacaaatagt aaaatcattt gaacaataga tactagccgt actgataatt
     3601 gatataacaa aacttgaaaa ataagatgac ctagaaatcc tcctaaaaca gaagaaaagg
     3661 ataccgtaaa aatcaatctc cattttatct ctttactttg tgaaaatctt ttgattgttg
     3721 aaataatcgc cattataaaa acagagaaac tagaataaaa agcaatatca ctgaccgaat
     3781 ggtatccaaa actatccata agtggcttaa ttataattcc accacccatg cctgatatag
     3841 aacccaatcc actagctatt ataatgatta gaaaataaat aatctcaacc actttttaag
     3901 tccatcctat tctttcaaac atctaaaacc tagatggcta ctagtggaag tagaaataca
     3961 tccatttcta gcagctactc ggtatcgatt acaataagaa caatgacata aaaaggaacc
     4021 tcctctgata gcatactcct catcttgtat cccatatttt ggtaactcat agtcatcttc
     4081 attaaaacta gctaataagg tatatctcgg attgcgacac cattcccaaa cattcccaat
     4141 catttgatat agaccaaaat cattaggtaa aaattcatag acaggagccg ttcctataaa
     4201 tccatctaag gcagtatttt cataaggaaa tcttccttgc caagtattgg catgataata
     4261 tccaccttct aaaagagtat ctccccaagg atattcagat tgtcttcctc ctctagcagc
     4321 atattcccac tgtgcttcag tcggtaaaga cattccgctc caattacaga aagccaatgc
     4381 atcttctaaa gcaacatgaa ctactggatg gtcttctaaa cccactagat tattgttctc
     4441 accgtaggga tgtttccagc aagcgttcga aacttgtaac caccacggag ccccagcagg
     4501 atgaggatat ccctcccttt ctgcctccgg aacgaacaat ataaaaacaa aagaccactc
     4561 ttgtcgctca gctagtgttt tatagcctgt ttctttcaca aattgagcaa attcttgatt
     4621 cgtaactgga gtatccgcaa tggaaaaatc ctttacacta acagcgactt gaggaccttc
     4681 aaaatcatct aaaaagccct cttcagaatt tgttcccatc ataaatgtcc cacccttgat
     4741 ttttttcatt ttcatgattg actatccccc gttttcgtta gagttggcgt tattttagaa
     4801 agttcaacag gaactaactc ttcatccaca acaaaacctt cttctctata tctaagaaag
     4861 tctactaatt ttttcttcat ctgacgaaca ataggttgat atttttcact tggatacaaa
     4921 tcattcattt catgcggatc ttttttcata tcaaataact gataatgatt taaaacagga
     4981 aaccagataa atttccactg gtctgtcaaa atatattgac tagaatcttt tcctaaagta
     5041 tgttcaccat gaaattctgt tcgccacccc tcgtactgtc caaataataa attcttaaca
     5101 ctccgtccat ctagttcatc tgtagtagtt cctcctgcca aatccacgag tgaaggaaaa
     5161 atatcttgaa ttttcactaa ttgcttgatt gttcccctat taccagctat caatccagcc
     5221 ggatcgtaaa taaacgaagg aatgtggata cttccctgat aaggatatcc ctttctaaac
     5281 agataatgtt caccaagctg atctccatga tccgaaacaa accagataat cgtatcttta
     5341 tcatgacgaa attcttttag ggcagtcaaa aagcgactaa tttgatggtc tatatgagta
     5401 atcaatccaa aataagcagc aaccattctt ctttgatcat cttcctttaa tttccctctt
     5461 aaagcataaa tagacggaat ttgcttttct aatacttccc aattcccgat atgcaaatcg
     5521 agaaattgag gcaagcgttc catatacata tcaaaatagt attttggggg atttaatgga
     5581 gcatgtggtt tttcaaaaga cattttgaga aaaaaaggaa ccgttggatc tcttctttgt
     5641 aaaaatgaaa tggactcact cacaacccag tttgttggat gtaacttctc atctttatcc
     5701 caaggtctag cttcccaaga attacaatcc attccgtcat caatcaaatc aacatcataa
     5761 ccaactttac cctttaaaaa ggctagatag tcactagcat aatcaaattg acttccataa
     5821 gccttatcat actttctatc aacatgtaga tacccatcgt gaagtaagac atgatcaaac
     5881 ccaagtctct gtctggaagg aaaaacatgc atcttaccaa tacactctgt ttgataaccc
     5941 atatctttaa agacttttgg aagagtatta gtaaaattcc aaggcacctc atcttgataa
     6001 ccaactcgtc cacttttatc ttggtctaag ccagtcaata aagctgccct agcaggaacg
     6061 caagaaggaa ctggagaata ggcattttca aaattataac ctacactagc catcatatct
     6121 aatgttggag tgctaactag cttatcctta ctattcaagg ataaggcgtc tgctctcatt
     6181 tgatctacaa caatcaaaat aatatttggc tgttttgtct gaaccataaa atctcctttc
     6241 taatatggca aaagaggcac aagaagatat ctacctttac tgcacccctt tctatatcaa
     6301 tctctctata taaaacaata acattcttgt tatgttttat agaaaaatga actaaaatat
     6361 gactaaatcg attaggaaat tcaaatcatt ttctagtact attttagaag ttacagtgta
     6421 ctattccaac ttcaataaac tataaacctt tgtctaataa caattttagt ggagataaga
     6481 aatcctacac ctaactcaat cttacacgta atctatttct attttatcac aaaaaacgca
     6541 agtaagacca ttaactcaat tcagttttat ctgccatttt cacaaatggg aaataagtca
     6601 agacactaat aatcaaacaa acaactgata agatgatggc acgccaatca aatgctgtag
     6661 agaagaaacc atataaaatt ggaggcatta cccaagtaac attttgtgta acaggtgaaa
     6721 caagacccca gcttgttgcc cagtaagcta ccgttgccat gaaaaccggg ctaagtacaa
     6781 atggtataaa tagcaaagga ttcaagacaa ctggtaaacc ataatttacc ggctcaccaa
     6841 tattaaacag aactggtgct agaccaagtt tagcaacttt tcgataatga ctgtttcttg
     6901 aaaaaattaa aatagcaagt accaatccta atcctccaaa ccagacaaac gccccaaaag
     6961 acccacttgt ccatatataa ggaatcggtt cacctttttg gaaagcatcc agattcgcta
     7021 acatagcaac tccaaacagc ccttccatga tgggagccaa tacatttcct ccatggagac
     7081 caaaaaacca gaataactta tttaaaaaga tcatcagaat aactgcaaag aaactttgag
     7141 acaaacctag taatggcgtt tgtaacacct tgtaaaccca atcaatcaat aagtaattgc
     7201 taagtaaatg gaaaacataa gtcaagatgg ctactatata catcgccata aatcctggaa
     7261 tgatagaagt gaacggctta gcaatcgcag ggggaactga atctggtaac ttgattaccc
     7321 agttcttttt cattacttta cagaaaataa tagaggctaa aaatccaatc atcatggctg
     7381 taaagtagcc tctggcatta atatggtttc ctggaatcac attcccaata gttaccatca
     7441 gatttttacc atcaaatgct agattatcaa ttccttgtaa gatttgatct aatttcacat
     7501 ctcctacatt tgccaaaggg aaactctttg taactgtact tccaatcgaa atgacaaacg
     7561 aagcaagcga taccaaacca gcagaaactg tatcaacctt ataaatctta gcgatattca
     7621 ctcccaagca atagatgaac aataaggaaa caattggtat acttcccttg aataccaaat
     7681 tattgatgtc aacaagccac tgaaaggttt tcgtaatact ttctaggtga aattgttgtg
     7741 gtaaatccac tagaaaagca tttaataaca aagcaatgga acctgtcata ataacaggca
     7801 tagtccccac aaatgaatca cgtaaagcaa ctaaaaactt ctgattccca actttcattg
     7861 caattggaga aagtttttct tgcattttat ccaacattgt attcatagca aattcctaat
     7921 ttttttcaaa tcgttcgtat acttctacca cttctttcgc caagtcaaca aaggtcagac
     7981 tcgtcattaa atgatcttgc gcgtgtacca tcaagaggct aattgatgtt tcttccccct
     8041 gtgcctcttt tgtcaacatt tctgtttgag ccttatgcgc ctgtaataag gcagcattcg
     8101 catcagctaa tcttcgattg gctttagaga aatcaccttt ttttgctgcc tgaatagcct
     8161 ccatagcatt acttttggct tccccaccat acataattag ccccattaca gattctaaat
     8221 tactttcatt catagcattc tcctcaatct tagtctaata aactttcagc taaatctaag
     8281 actttagaac cattcatcat tccgtaatct gtcatcggaa taaccgccac aggaatctgt
     8341 ctatctttta gtttttcttg aaagtcccct agtaaatagc gaacttgagg acctaaaagc
     8401 aacacattta cttcttttgt tgctacaatt tcttctgctt caggagctgg aactgcaaaa
     8461 atttctgcat ccaacccctt atcttctgct gccttttgca tctttgtcac tagcagactt
     8521 gtactcatac ctgctgcaca cgctaacata attgttactt tagccatagc ttactccttt
     8581 atcacctatt tactgaccta gccaagcgag atactgtatg tctcactcct ctaattgcct
     8641 tgctcaatgc ataaatatta tcgactgttg ccagtccccc atatcctgcg tcacctatat
     8701 gttgaatatc aactccacaa attttatttc taagtgcaat ttccttgata gtatctgtat
     8761 cagatgtttc ttggctagta ccaatagcac tcagtactag cccaccctta ctatgaacga
     8821 gatcaacgac ttcacgcaac tcttggtcat gaaaggctgg aacggttcca actgctggaa
     8881 caagtatcac atccgcacct gcttccaaca attgctctgc gacagaaagc tctgccacag
     8941 gctcattcac tcctgccccg tgcatcttac ctgcaataat caaaccagaa aagttttcct
     9001 tagcagtttg aacagtctta atgatttctc gattgctaac tcctgttcca ggatttccag
     9061 tcaaacagac aaagtcaaaa cctaactcct ctattcgctt caatgtttca acactagcaa
     9121 cacgacccgc aacaatttcc tgtgtttcct ctagcatctt tgcagatggg tcaatcggtt
     9181 ccaaatttac accaattgga caagcaacaa gctggtgtaa gcgatgaata acatcatcac
     9241 ctgaactatc caaagcataa attttaggct caaaaacatc aacacaattc aataaaatca
     9301 tatcagctcc aaaagctcta gcaatttcag aattagttat atccccaaca aatgtctcac
     9361 gagtaactac attttcagat agaacaactc taccttcact tgctaaaata ctttgtttta
     9421 attcttcagc attcatctgt aatatctcag atggatttgc actaattaaa cgtttcattc
     9481 tcaatcctcc aataacttaa accgtttttc aatagctatt acaaatggta aataaagtaa
     9541 gacatccaaa acaatcaata caatttgtaa taatgaacca gaaatacttc ctgtagcaag
     9601 taaaccactt ataacaggtg gcatagtcca acctggatct gtataagtta gcggaaccaa
     9661 acctgatgcc attgctcccc acgctactag taagttaaac attggagcta agataaatgg
     9721 aactagtaac aaaatgttca aaacaatagg aacgccaaac attgctggtt catttatatt
     9781 aaataatcca ggtataacag ttataggtgc caaagttttt agttgtttac tagctttctt
     9841 cttatgtgct aaatatccta acgcaagaac taaacctatg gttgcaccac cgccacctat
     9901 gaatacaaaa ttatccataa atacgctagt aaaaatattt ggaagagttt ctccagtttg
     9961 ataagctact ttatttgcat ctagattagc taaccataac ggtttcataa ctgcattaac
    10021 aacatttcct ccgtgtaatc ctacaaacca aaaactacta ttaaggaaaa ttaagataag
    10081 aagaccaata acattatttc ctaaaagtcc aagtggacct cccaaagctc cttgagcaat
    10141 ttcatgaaga ttaggtaaac ttaatttatc taaagttgca taaacaatca accaaccaac
    10201 aatagtcact gcaccaggaa taatggcact aaagctttta gatactgctg gtggaacacc
    10261 atccggcatt tttatctgta tattatgatt tataaaccat tggtagatat agccattaat
    10321 caatcctaaa atcatagcaa caaataaacc ttttgatgcc atgaaagcgg taggcattcc
    10381 tgctccagcc tctcctgtaa tgaaaggcgt aactgtaata aaagatgata atgacacaat
    10441 accagcagga ataccatcaa ctttgtattg tctagccata aagtaagcga taccaaaact
    10501 agctacaaga ccaagtaatc caaaactact atcaacaccc ttccataaat agtcagcaac
    10561 accaatatca cctagatatt tttcccatcc tggaatagga aaacttgcaa taaccattag
    10621 taatgaacct atcaacaata aaggtatagt tagagtaata ccgtctcgaa tagaaaccaa
    10681 cgctttatta ttacccaatc tagccgctac tggaagtaac ttttcttgta ataatttttc
    10741 cataaccttt tcctcttaaa tatattttat tatcaaaagt tatcaattat ctttattttt
    10801 tgacgtcaag ttattatttg attatctttt gttatttata ttttacaact ataagaaagc
    10861 cttgtcaagg atttgtgtaa atatttgatt attttttatt atcaatatat aataaaaaga
    10921 gaccagtaag caatgaaaag cttaaaaaga ttaacatcta catttatcca tagcaaaaaa
    10981 tcataggatt ttaagaatct gacaactagg aagaaaacta atatgacctt atctaagaaa
    11041 caattacaac taagagctaa aatactggaa acagtttaca cattaggtcc catttcacga
    11101 attgaaatcg ctactaaaac aggaataaca cctgcaacaa ctagcagtat cacaaatgat
    11161 ctgattaaag aaaatattct cttagaactt ggtgaagacg aacacgatac aagcgtaggg
    11221 agaaaaaaaa tcctgctaga cattcaagct aaacgatttt actacattgg ttgtgaacta
    11281 tctgaaaaac atttcacttt tgctttagga gataatctag gaaatatctt aaaggaagag
    11341 aaagaaattg ttactaaaca gttaatccaa gaaaagggaa atcaactcat taaccagaca
    11401 ctaaagcaat tcttaaataa ctgctctgat tatgaaatag aagcaattgg aatcgcgcta
    11461 cctggtcgct atttagatga ttataaaata acaacaaaca atcctttatg gcaacatatc
    11521 gacttagaaa tgattcaatc ccactttgat aagcctttgt ttttctctaa caatgttaac
    11581 tgtatggcta taggtaaacg cctatttagt cgccaacaga atgaccctaa ttttgcttat
    11641 tttcattttg ctagaggtat gcactgttct tatatctacg acggaaacat ttatgggaaa
    11701 ggaaatctaa tgattggaga aatagggcat acagttgttt catctgaggg agaagaatgt
    11761 agctgcgggc gcaagggatg cttacaaact tttgctggag aatcttggtt aatcaaaaaa
    11821 tcaaaaattc tatatcatca atctccctat tctctacttc ctagtttagt caaaaatgcc
    11881 gatgatattg atatccaagt tattttaacc gcttatcaat taggtgacac tggaataatc
    11941 acacttatcc atcaagcctt actctatctt agtcaaacca ttttaaatat cagtatgatg
    12001 attgactctc aaaaaatcta cctacatagt cctttactaa caaatcaaca catcatccaa
    12061 aaactatata gtgaaatgaa ctacaaaccc aaactgctct ataatcgcct acctgaagtc
    12121 attattgaac cttacaatga ttttacagcc gcacactcag ctattgcact ttgtttgtat
    12181 catacaattc tacattctta attttctatt acatttaata aataggctaa aaagagggag
    12241 tatatcataa aaagcgaata aacctgtttt ctgaaaatca gaattctgtt ttgttcgctt
    12301 tctttatatt tggaattata ctcctatctc ccctttttca taatgtatag tgaaatgaaa
    12361 taataacagg acgaatcgat c
//
LOCUS       DAVKJJ010000054        12350 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000054 DAVKJJ010000000
VERSION     DAVKJJ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12350
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.054"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            117..926
                     /locus_tag="VW144_001688"
     CDS             117..926
                     /locus_tag="VW144_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU9601104.1"
                     /translation="MLLQKELIPMIEANLPNMAYAEKDIAKFFLKQQPLNDYSSKALC
                     EYLNVSKATLTRFAKKCGFKGFRQFIFKYQEMIHEKEKLALYTEATEKVLSDYEEMLR
                     KTYTVLDEVQLERIAEMIETAERVYLYGKGSSVLALQEMKMRFMRLGVIGEVLSDEDM
                     ILWSSLLLNENCLVIGASISGQTDIVLEGLQKAADKGAKTVLMTTRKFDEEDCFFDEL
                     LLLASTDHLSYGNRISPQFPILLITDCLFSNYLESPERQYYYNQTIIHKEE"
     gene            931..1632
                     /locus_tag="VW144_001689"
     CDS             931..1632
                     /locus_tag="VW144_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU9601105.1"
                     /translation="MSQIWTKEKFISQVQGGVIVSCQALPGEALYNEEFSLMPFMAKA
                     ALEAGAVGIRANSVRDIKAIQKVVDLPIIGIIKRDYPPQEPYITATMKEVDELVECGT
                     TVIAFDATLRPRYDGLVVSEFIKKIKEKYPNQLLMADVSNLDEGLYAFKSGVDFVGTT
                     LSGYTSTSVQSDEPDFELMKKLADFNIPVIAEGKIHYPEQLKKAYSLGVTSVVIGGAI
                     TRPKEIAQRFINVIK"
     gene            1657..2574
                     /locus_tag="VW144_001690"
     CDS             1657..2574
                     /locus_tag="VW144_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HEU9601106.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQDRVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHIACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAASNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            2602..4143
                     /locus_tag="VW144_001691"
     CDS             2602..4143
                     /locus_tag="VW144_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEU9601107.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGSSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGVVAIVYSYTGGLKSVLWTDFIQGVILISGVVLALFVLIANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLATATVFYLIGTGLYVFYQVQNADSAASNIPQDQIFMYFIAYQLPVGITGL
                     ILAAIYAASQSTISTGLNSVATSWTLDIQDVISKNMSDNRRTKIAQFVSLAVGLFSIG
                     VSIVMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTGNKVSAPKYTTIHDITEIKADSS
                     WEVRH"
     gene            4162..4620
                     /locus_tag="VW144_001692"
     CDS             4162..4620
                     /locus_tag="VW144_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9601108.1"
                     /translation="MIISEQSDFKRYASVNKYFSKVCDFLENTNLTDLVDGKIDIDGE
                     NVFANCMTYLADGVPGDIFETHKKYLDIHIVVENTEKMAVTSPVRAQSRVPFSEEKDI
                     AFYDSKDYQIVELLPGNMLVTFEEDLHQPKIHCNDETVKKLVIKVLNEEK"
     gene            4622..6844
                     /locus_tag="VW144_001693"
     CDS             4622..6844
                     /locus_tag="VW144_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEU9601109.1"
                     /translation="MKKNIKQYVTLGTVVILSAFVANSVAAQETETSEVSTPKLVQPV
                     APTTPISEVQPTSDNSSEVTVQPRTVETTVKDPSSTAEETPVLEKNNVTLTGGGENVT
                     KELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNNLVSRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPVPLEWPREVGGRDLQISGGATYIDSVIVEKKNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLRKQGDTDYNYTIRENGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFENNKKTEYRNGTKVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSKDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQIWIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN
                     "
     gene            6884..7993
                     /locus_tag="VW144_001694"
     CDS             6884..7993
                     /locus_tag="VW144_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9601110.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEFEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            8150..9040
                     /locus_tag="VW144_001695"
     CDS             8150..9040
                     /locus_tag="VW144_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9601111.1"
                     /translation="MKIIGIDIGGTTIKADLYDEFGTSLNHFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYTLNHSIDGVGISTAGVVNANTGEIIYAGYTIPGYIGVNFTAEIEKRF
                     GLYTFVENDVNCAALGELWKGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLRSGDKVAEETFEIFVE
                     NLTKGLLTISYLLNPEILILGGGILDSKDILLPEIQSSLAKNAMDNRFLPKNLVAATL
                     GNEAGRIGAVKNFLDRISNK"
     gene            9065..9388
                     /locus_tag="VW144_001696"
     CDS             9065..9388
                     /locus_tag="VW144_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601112.1"
                     /translation="MTNSVFSTMQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKS
                     YDEKSHEELVSIESNLEMKQQNLIDEVNKTIKENDANIQYISSSRRGEFVEKIIGRVV
                     EKYGH"
     gene            9378..11369
                     /locus_tag="VW144_001697"
     CDS             9378..11369
                     /locus_tag="VW144_001697"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEU9601113.1"
                     /translation="MAISQMKRISLLFSKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFPTIQIFHTSNSNHGVIEGNDALTYLMNQQQHLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNHQINRVHELERHIQSNNEEIERLIKW
                     EKLEIVPANLEQFSFCKGKVGTIPRTEDNRLYNSLLENNIEVQEIFSNDREYGVVVFY
                     QSSYSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITEKENIIASLQDSKKYLID
                     LQWQIDYILSIYARQISKNNFLCTPHLVALEGWIEETRILYFIKVMDEHFGHSIYIYE
                     SETLTDNQDEIPIKLTNHSLIEPFELLTEMYALPKYYEKDPTPVLAPFYFTFFGMMVA
                     DLGYGLLLFLGTMLALKIFHLPSATKRFLKFFNILGVAVAIWGGIYGSFFGYELPFHL
                     ISTTSDVMTILVVSVVFGFITVFAGLLASGLQKVRMKKYAEAYNSGFAWCVILLGLLF
                     IAVGMLMPDMRPLFVLGKWVSIFNAVGILIVSIIQTKSLSGIGAGLFNLYNISSYIGD
                     LVSFTRLMALGLSGASIASAFNLIVGLFPGILAKLTIGLVLFILLHAINIFLSLLSGY
                     VHGARLIFVEFFGKFYEGGGKPFQPLKASEKYIKVITKN"
     gene            11385..11861
                     /locus_tag="VW144_001698"
     CDS             11385..11861
                     /locus_tag="VW144_001698"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEU9601114.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            11895..>12350
                     /locus_tag="VW144_001699"
     CDS             11895..>12350
                     /locus_tag="VW144_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601115.1"
                     /translation="MSNLENLRESVIEQAHEKGRMKLLDSKKKIDDEFEMQKSLIIKK
                     KEAEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALLEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNYLFSEQPI"
BASE COUNT         4197 a         1771 c         2439 g         3943 t
ORIGIN      
        1 ttctgttatg aaacgaaaga aattggaaac tagtttctaa tttatgaaaa atattgaaaa
       61 aaaaaaaaag tgttaaaata aagaatgtaa acgtttactt caactaagga gctcatatgt
      121 tactgcaaaa agaactaatt ccaatgatag aagctaactt accaaatatg gcatatgctg
      181 aaaaagacat tgctaaattc ttcttaaaac agcaacctct gaatgattat tcatctaaag
      241 cattgtgcga ataccttaat gtatccaaag caacattgac tcgatttgcg aaaaaatgtg
      301 gttttaaagg ttttagacaa ttcattttca aataccaaga gatgattcat gagaaagaaa
      361 agttggcatt atatacagag gcaacagaaa aagttttatc cgactatgag gaaatgttga
      421 gaaaaactta cacggttctt gatgaagttc aacttgagcg tattgctgag atgatagaaa
      481 ctgctgagcg tgtatatctc tacggtaaag gaagttctgt tcttgcttta caagaaatga
      541 agatgagatt tatgcgtctc ggagtgattg gtgaagtatt atcagacgag gatatgattt
      601 tgtggagtag cttactactt aatgaaaatt gccttgtcat tggagcatcc atttcaggtc
      661 aaactgatat tgtactagaa ggtctacaaa aagctgcaga taaaggcgct aaaacagttt
      721 taatgactac aagaaaattt gacgaagaag attgtttctt tgatgaacta ttgttattag
      781 cttcgaccga tcatctctcg tatggcaatc gcatatcacc tcagtttcca atacttttaa
      841 ttacagactg cttattctct aattatctgg aaagtccaga gagacaatat tattacaatc
      901 aaactattat ccataaggag gaataataaa atgagccaaa tttggactaa agaaaaattt
      961 ataagccaag ttcaaggtgg agtcattgtt tcttgtcaag ctttacctgg tgaagccctt
     1021 tataatgaag aatttagctt gatgcctttt atggctaaag cagctttaga ggcaggagca
     1081 gtgggcattc gcgcaaattc tgtgcgtgat attaaagcaa ttcagaaagt agtagattta
     1141 ccaataattg gaattatcaa aagggattat ccacctcaag aaccatatat tactgctacg
     1201 atgaaagaag tagatgaact tgtagaatgc ggaacaacag tcattgcatt tgatgcaact
     1261 ttaagaccaa gatatgatgg cttagttgtc agtgaattta tcaaaaaaat aaaagaaaaa
     1321 tatccgaatc aattgctgat ggcggatgta agtaatttag atgaaggtct ctatgcattt
     1381 aaatcaggcg ttgattttgt tggtacaaca ttatcaggtt acacaagtac aagtgtacaa
     1441 tcagatgagc ctgattttga actaatgaaa aaattggctg attttaatat tccggtaatt
     1501 gccgaaggaa aaattcatta tccagaacaa ttaaaaaaag cttatagttt aggtgttacc
     1561 agtgtagtca ttggtggagc gattacacgt ccaaaagaaa ttgctcagcg atttattaat
     1621 gtcatcaaat aaaaaacaat ataaaggaga aatctgatga aagatttaac taaatacaaa
     1681 ggcgttatcc ctgcatttta tgcttgctat gatgaaaatg gtgaaattag ccaagatcgt
     1741 gtaaaatctc tggtacaata tttcattgac aaaggtgtaa aaggtatcta tgtaaatggt
     1801 tcttcaggtg aatgtattta ccaaagtgta gaagatcgta aacaaattat tgaagctgtt
     1861 atggaagttg ctaaaggtaa attaacagtt atcaaccata ttgcatgtaa taacacgaaa
     1921 gatagtatcg aattggcaaa acattcagaa agtgttggag tcgatgctat tgcagctatc
     1981 ccacctattt atttcaaatt gccagagtat tcaatcgcag catattggaa tgcaatgagt
     2041 gaagctgcgt caaatacaga ttttattatc tataatattc cacaattggc aggggttgcg
     2101 ttgactggta gtttgtatgc aacaatgcgt caaaatcctc gtgtgattgg agttaaaaat
     2161 tcttctatgc ctgtacaaga tattcaaatg tttgtagctg caggtggaga agattacatt
     2221 gtattcaatg gtccagatga acaatatctt ggtggtcgct tgatgggagc agaagctggt
     2281 attggtggta cttatggcgt tatgccagat ttgttcttga aattggaaag tttgattcaa
     2341 gaacgagatt tagatacagc taaaaaactt caatatgcta tcaatgaagt tatctataag
     2401 atgatatcag gtaaggcaaa tatgtatgct gtagcaaaag aagttttgcg tctaaatgaa
     2461 aaacttgatt taggttctgt tcgtcaacct ttagaagcat tggcagaagg tgacttggaa
     2521 gttgcaaaac aagcagcaga acttattcaa caagcacgaa aagaattttt ataataaata
     2581 aaatcaatat ggagatatgt tatgggcaca acaggattta caataattga cttaattatc
     2641 ttgattgttt atttacttgc ggtgttggtt gcaggtatct atttctctaa aaaagagatg
     2701 aaaggaaaag agttctttaa aggagatggt tcggttcctt ggtatgttac ttcggtatcc
     2761 atttttgcca caatgctcag tccgatttcc ttcttgggac tcgctggtag ctcttatgca
     2821 ggtagctgga ttttatggtt tgctcaatta gggatggtag tagctattcc actgacaatt
     2881 cgttttatct tacctatctt tgcacggata gacatcgata cggcatatga ttacttggat
     2941 aaacgtttta attctaaagc acttcgtatt atttcagcac tcttgtttat tatttatcaa
     3001 ttgggacgta tgtctatcat tatgtacctc ccatcagctg gtttatcagt attgacagga
     3061 attgacatca atattttgat tattttgatg ggtgtagttg caattgttta ttcttatact
     3121 ggtggtctaa aatccgtatt atggacagac tttattcaag gtgtgattct gattagtggt
     3181 gtcgttttag ctttatttgt actgattgct aatattaaag gtggctttgg tgcagtagca
     3241 gaaacattag caaacgggaa attccttgct gcaaatgaaa aacttttcga tcctaacttg
     3301 ctttcaaact ccatcttttt aattgtgatg ggttcaggct ttacaatctt gtcttcctat
     3361 gcttcatctc aagatttggt tcaacgtttt actacaacac aaaatattaa gaaacttaat
     3421 aagatgttgt tcacaaacgg tgttttgtca cttgcaactg caacagtctt ttacttgatt
     3481 ggtacaggct tgtacgtatt ctatcaagta caaaatgcag atagtgcagc tagcaatatc
     3541 cctcaagacc aaatctttat gtactttatt gcataccagt taccagtagg tatcacaggt
     3601 ttgatcttgg cagcgattta tgcagcatct caatcaacta tttcaacagg tttgaactct
     3661 gttgcaactt catggacatt ggatattcaa gatgtcattt ctaaaaatat gtcagacaat
     3721 cgtcgtacga aaattgcaca attcgtatct ctagcagtag gtttattctc aattggtgtt
     3781 tccattgtca tggctcactc agatattaaa tctgcatacg aatggttcaa tagtttcatg
     3841 ggacttgtac ttggtctact tggtggtgta tttattcttg gatttgtttc taaaaaagca
     3901 aataaacaag gtgcttatgc agcgctgatt gtatcaacca tcgtcatggt atttattaaa
     3961 tacttccttc ctccaacagc tgttagctac tgggcatatt cattgatttc aatctctgta
     4021 tcagtagttt caggttatat tgtatctgtt cttactggaa ataaagtatc tgcacctaaa
     4081 tatacaacga ttcatgatat tacagaaatt aaagcggatt caagttggga agttcgtcac
     4141 taatagaaaa gagaaaataa tatgattatt tctgaacaat ctgattttaa acgatatgct
     4201 tctgtcaata aatatttttc aaaagtctgt gattttttag aaaatacaaa tttgacagat
     4261 ttagttgatg gaaaaattga tattgacggg gaaaatgttt ttgcaaattg tatgacttat
     4321 ctagctgatg gagttccagg agatattttt gaaactcata agaaatattt agatattcat
     4381 attgttgttg aaaatactga aaaaatggca gtaacttccc cagttcgtgc ccagtcacgt
     4441 gtacctttta gcgaagagaa ggatattgct ttttacgata gcaaagacta tcaaattgtt
     4501 gaattgcttc cagggaatat gttggtgact tttgaagaag atcttcacca accaaagatt
     4561 cattgcaatg atgaaactgt taaaaaactt gttatcaaag ttttaaacga ggaaaaataa
     4621 aatgaaaaaa aatattaaac aatatgtaac cttaggtact gtagtgatat tatcagcatt
     4681 tgttgctaac tcagttgcag ctcaggagac tgaaacttct gaagtatcaa caccaaagtt
     4741 ggtgcaacct gttgcaccaa cgactccgat ttcggaagta caacctacat cggataactc
     4801 ttcggaagtt actgtacaac ctcgaacagt tgaaactact gttaaggatc catcttctac
     4861 agcggaagaa actcctgtct tagaaaaaaa taatgttact ttaacagggg gcggagaaaa
     4921 tgttactaaa gagttaaagg ataaatttac tagcggtgac tttactgtag tgattaagta
     4981 caatcagtca agtgagaaag gcttacaagc tctgtttgga atatctaatt ccaaacccgg
     5041 tcaacaaaat agttatgtag atgtgttcct tagagacaat ggtgagttgg ggatggaagc
     5101 gcgtgatact tcttccaata aaaataacct agtatccaga cctgcttcag tttggggtaa
     5161 gtacaaacaa gaggctgtga ctaacactgt tgcagtagta gcagattcag tcaaaaaaac
     5221 atattcttta tacgcaaatg gtacaaaagt agtagaaaag aaagtggata atttcctaaa
     5281 catcaaggat attaaaggta ttgattacta tatgcttggg ggagtgaaac gtgcaggaaa
     5341 aacggcgttt ggttttaacg gaacactaga aaatatcaaa ttctttaata gtgcattgga
     5401 tgaagaaact gttaaaaaga tgacaacaaa cgctgttact ggacatttaa tttatacggc
     5461 taatgataca acaggttcta actatttccg tattccagtt ctgtatactt ttagcaatgg
     5521 tcgggtattt tcaagcattg acgctcgtta cggtggaact catgatttct tgaataaaat
     5581 taatattgct acaagttata gtgatgataa tggtaagaca tggactaaac caaaattaac
     5641 attggcattc gatgattttg cgccagtacc attagaatgg cctcgtgaag ttggtggacg
     5701 tgacttacaa atcagcggtg gtgcaaccta tattgactct gttattgttg aaaaaaagaa
     5761 caaacaagta ctcatgtttg ctgatgtgat gcctgctgga gtaagtttta gagaagcaac
     5821 tagaaaagat tcaggttata aacaaattga tggtaattat taccttaaat taaggaaaca
     5881 aggtgatact gattacaatt atactattcg tgagaatggt actgtatacg acgatcgtac
     5941 caacagacca actgaatttt cagtagataa aaatttcggt attaaacaaa atggtaatta
     6001 tttgacggta gagcaatatt cggtttcatt tgaaaataat aaaaagactg aatatcgtaa
     6061 tgggactaaa gtccatatga atatcttcta caaagatgca ttgtttaaag tagtgccaac
     6121 gaactatatt gcatatattt ctagcaatga tcatggcgaa tcttggtctg caccaacttt
     6181 attacctcct ataatgggac ttaatcggaa tgcgccatat ttaggtcctg gacgtggaat
     6241 cattgaaagc tcaactggac gtattcttat tccgtcttac actggtaaag agtctgcgtt
     6301 catttatagt gacgataatg gagcatcttg gaaagttaaa gtagtgccac ttccttctag
     6361 ttggtcagca gaagcacaat ttgtagaatt gagtccagga gtaattcaag catatatgcg
     6421 tacaaataat ggtaaaattg catatttaac aagtaaagac gcaggtacta cttggagtgc
     6481 accggaatat ttgaaatttg tttcaaatcc aagttatgga acacaattat caatcatcaa
     6541 ttatagccaa ttgattgatg gtaaaaaggc tgtcatttta agtactccaa actccacaaa
     6601 tggtcgtaaa cacggacaaa tttggattgg tctaattaat gatgataata caattgattg
     6661 gcgttatcat cacgacgttg attatagtaa ctatggatac tcatattcaa cattgacaga
     6721 gttaccaaat catgaaattg gattgatgtt tgaaaaattt gattcatggt ctcgtaatga
     6781 acttcatatg aaaaatgttg taccatatat aacatttaag attgaagatc tgaaaaagaa
     6841 ttaaagctga aatttgaaaa tatataaaaa gaggataaaa attatggtaa attacggtat
     6901 tgttggagct ggatattttg gagctgattt agctcgctca atgaacaaaa ttgaagatgc
     6961 aaaagtggtt gcggtatttg acccaaatca tggagaagaa gttgctcaag agttgggatc
     7021 agatgtttgt gcaagtttag atgaacttgt agcacgtgaa gatattgatt gtgtgatcgt
     7081 agcttcacct agctaccttc accgtgaacc agttgtgaaa gctgctcaac atggcaaaca
     7141 cgtattttgt gaaaagccaa ttgcattgtc ttatgaagat tgtaaagcca tggttgacgc
     7201 atgtaaagaa aataatgtca tctttatggc tggtcacatc atgaacttct ttaacggtgt
     7261 acaccatgct aaagaattga ttactcaagg taaaatcggt aaagttcttt attgccatgc
     7321 tgctcgtaca ggttgggaag aacaacaacc aactgtatca tggaagaaac ttcgttctca
     7381 atctggagga catttgtacc accatattca tgaattagat tgcattcagt ttatcatggg
     7441 aggacttcct gaaaaagcga caatggtagg aggcaatgta tatcataaag gtgaaaactt
     7501 tggtgatgaa gatgatatgc tcattgtaaa cttagaatac tctgatgatc gttatgctgt
     7561 tttggaatat ggtaatgctt tccgttgggg tgaacactac gtcttgattc aaggaactga
     7621 aggagctatc aaacttgact tgttcaatac tggcggtact cttcgtgtta aaggtgaagg
     7681 agaatcacac ttcttagttc atgaaactca agaggaagat gatgatcgta cagctatcta
     7741 taccggtcgt ggtatggatg gagcaattgc gtacggtaaa ccaggagtac gttgcccatt
     7801 atggttgcaa acatgtattg ataaagaaat ggaatatcta catgacatca ttaaaggtgg
     7861 agaaattaca gaagaatttg aaaaacttct caatggtgta gctgctttag aatcaatcgc
     7921 taccgctgat gcatgtactt tatcagttaa agaagatcga aaagtaagtc tttcagaaat
     7981 cacaaatgct taacttttgt aaaacagaat agtaaattct tgtcattata taatttctaa
     8041 agttctgtga tacaactcat tgaataaaga aatagagatg ggactgggat aatgcccagt
     8101 cccatttttt atcaaaaagt aatgagatca aaaatgtggg agtgttgaaa tgaagattat
     8161 aggtatcgat attggcggaa caacaattaa ggcagattta tacgatgagt ttggaacgag
     8221 tttgaatcat ttcaaagaga tagaaacaat tattgactat gatttgggaa cgaatcagat
     8281 attaaatcag gtctgtgatt taattggtga gtatacttta aatcattcaa ttgatggtgt
     8341 tgggatttcc actgctggag ttgttaatgc taatactgga gaaatcatct atgcaggcta
     8401 tacaatacca gggtatatcg gagtaaactt tactgccgaa atagaaaaac gttttgggtt
     8461 gtatactttt gttgaaaatg atgttaattg tgctgcatta ggtgaattgt ggaagggaca
     8521 agccaaagat aagaaaaatg tagtaatggt tactattgga acaggtatag gaggcagtat
     8581 tattgtcaac ggacaaattg ttaacggatt taactatact gctggtgaag taggttatat
     8641 tcctgtaggt aattcggatt ggcaaagtaa agcctcaaca accgcattga ttcatttata
     8701 tcaaaaaaag agcttgaaaa ctaatcaaac tggacgtact ttcttcactg atttaagatc
     8761 tggagataaa gttgctgaag aaacttttga aatttttgta gaaaatctaa caaaaggttt
     8821 attaacgatt tcttatctac ttaatccaga aattctcata ttaggaggtg ggattctgga
     8881 tagtaaggat attttgttac ctgaaattca aagttcttta gctaaaaatg caatggataa
     8941 taggttttta cctaaaaatc ttgtggcagc tacattagga aatgaagctg gtcgtatagg
     9001 agctgtaaaa aatttcttag atagaatttc taataaatag tatgtaagat aaggaggtgt
     9061 cacaatgact aactctgtat tttcgacaat gcaagatatt gagaatgttg caaccgatat
     9121 tataaaatca tatgataatg agatttatac ttataaagct gtttcccaag aagaattgga
     9181 aaaactagaa aaaagttatg atgaaaaaag tcacgaagaa ttagtttcaa tagaaagcaa
     9241 tttagaaatg aaacaacaga accttattga tgaggttaat aaaacaatca aggaaaatga
     9301 tgcaaatatt cagtatattt catcaagtag gagaggagaa tttgtagaaa aaattattgg
     9361 tagggtggta gaaaaatatg gccattagtc agatgaaaag aatctctcta ctattttcta
     9421 aaagtagtct tgatgatgtt ttaaaaacta ttcaagaact agagtcagtg cagttccgtg
     9481 atttaaaggt tcaggataac tggtcagaag ctctagaaaa agatgaagtt gtatttccaa
     9541 ctattcaaat ttttcatact tctaattcca atcatggggt tattgaggga aatgatgcct
     9601 tgacttattt gatgaatcaa caacaacatt tagaagcaac tgtagagaaa ttacaagaat
     9661 acctaccgaa agaaaacacg tttaaattat tgcagcaacc tccgataact acctcttatg
     9721 aagaattaga gaaatttggt aaagctaatg ttgctgaggg tgttcttaaa aaagtgaatc
     9781 atcaaattaa cagagttcat gaattagaaa gacacattca aagtaataat gaggaaatag
     9841 agcgattaat aaagtgggaa aaattagaaa ttgttcctgc gaatttagaa caattttctt
     9901 tctgtaaagg aaaagtcgga acaattccaa ggactgaaga taatcgctta tacaatagtc
     9961 ttttagaaaa caatattgaa gttcaagaaa tattttctaa tgatagagag tacggtgttg
    10021 ttgttttcta tcagtctagt tactctatag attttgatga atacttattt gaaccatttg
    10081 attattctag aaaggaatta ccgaagcagc gagtagtaga tttagatcaa gaaaacatgc
    10141 agttaataac tgaaaaagag aatattatcg catcgttgca agattcaaag aaatatttga
    10201 tagatttaca atggcaaata gactatattt tatctatcta tgctcgtcaa atctctaaga
    10261 ataacttttt gtgcactccg catctagttg cattagaagg atggatagaa gaaactcgta
    10321 ttttatattt tataaaagtt atggatgagc attttggaca ttctatttat atttatgaat
    10381 cggaaacatt gacggataat caagatgaaa tacctatcaa attaacgaat cattctttaa
    10441 ttgaaccatt tgaattattg acagaaatgt atgctctgcc caaatattat gagaaagatc
    10501 ctacacctgt attagcacca ttttacttta cattttttgg aatgatggtt gctgatttag
    10561 gctatggttt actattgttt ttaggaacaa tgttagcatt aaaaattttt catctacctt
    10621 cagcaactaa gagattttta aaattcttta atatattagg ggtagccgtt gcaatttggg
    10681 gtggaatcta tggctcattt tttggatatg agttgccatt tcatctgata tctacaacct
    10741 ctgatgtcat gactatatta gtagtgtcag ttgtgtttgg gtttattaca gtatttgcag
    10801 gtttgttagc ttcaggacta caaaaagtaa gaatgaagaa atatgcagaa gcatataatt
    10861 caggatttgc gtggtgtgtt attctgcttg gcttgttatt tattgctgtt ggaatgttga
    10921 tgcctgatat gagaccgtta tttgtattag ggaaatgggt atctattttt aatgctgtgg
    10981 ggattttgat tgtttctatt attcaaacca aaagcttgtc aggtattgga gcaggattgt
    11041 ttaatctata taacatttca tcttatatag gtgatttagt tagtttcact cgattgatgg
    11101 cattaggatt atctggagca agtatagcat cagctttcaa tttaattgtt ggtttgtttc
    11161 cgggaatatt ggctaaactg acaattggat tagtattatt cattctttta catgcgatca
    11221 atatttttct atcgttacta tcaggatatg ttcatggagc acgtctgata tttgttgaat
    11281 tttttggtaa gttttatgag ggtggaggaa aaccatttca acctttgaag gcttctgaga
    11341 aatatattaa ggttattaca aagaattaat ggaggatata tataatggaa catttagcaa
    11401 cttatttttc aacctatgga ggagctttct tcgctgcatt gggaattgta ttggcggttg
    11461 gattaagcgg tatggggtct gcttatggag ttggtaaggc tgggcaatct gccgcagctt
    11521 tactgaaaga acagcctgaa aagtttgcct cagctttgat attgcaatta ttgcccggaa
    11581 cacaaggatt atatggtttt gttattggaa ttttaatttg gttgcaatta actccagaac
    11641 ttcctttaga aaaaggcgtt gcttatttct ttgtagctct tccaattgct attgtaggat
    11701 acttttcagc taagcatcaa ggaaatgtag cagtagcggg aatgcaaatc ttggctaaaa
    11761 gaccaaaaga attcatgaag ggagcaattt tagctgccat ggtagaaacc tatgcaattc
    11821 ttgcttttgt cgtatcattc attttgaccc ttcgtgtata agaaataaat ttgcaattca
    11881 aaggaggtgt ctaaatgagc aatttagaaa acttacgaga gtctgttatt gaacaagctc
    11941 atgaaaaagg gcgtatgaaa ttattggatt ccaaaaagaa gattgatgat gaatttgaaa
    12001 tgcaaaagtc gctcattata aagaaaaaag aagctgaaca tgaacgaaag ttaaaagaat
    12061 tgcaacagaa atatcaaata atttttcaac aattaaaaaa taaggaacgc caatcaacgt
    12121 tagtatcaaa acagaaaata ttaaaagaac tttttcaatc tgctttacta gaaatggaat
    12181 cttggagtgc agataaagaa atggagttca tctatcgaat tctggaacga tattcacaac
    12241 aagaggtcat agtaaccttt ggggaacgga ctttagctaa attcaatttg gaacaattag
    12301 agaaattgaa attctctttt ccaaattatt tatttagtga acaacctatc
//
LOCUS       DAVKJJ010000055        11888 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000055 DAVKJJ010000000
VERSION     DAVKJJ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11888
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.055"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            16..1017
                     /locus_tag="VW144_001700"
     CDS             16..1017
                     /locus_tag="VW144_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9601116.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            1625..1702
                     /locus_tag="VW144_001701"
                     /pseudo
     CDS             1625..1702
                     /locus_tag="VW144_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            2158..3519
                     /gene="trpE"
                     /locus_tag="VW144_001702"
     CDS             2158..3519
                     /gene="trpE"
                     /locus_tag="VW144_001702"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU9601117.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPVPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSDYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKTLATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMKVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            3516..4082
                     /locus_tag="VW144_001703"
     CDS             3516..4082
                     /locus_tag="VW144_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU9601118.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEEVVK"
     gene            4093..5097
                     /gene="trpD"
                     /locus_tag="VW144_001704"
     CDS             4093..5097
                     /gene="trpD"
                     /locus_tag="VW144_001704"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU9601119.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNLGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            5094..5861
                     /gene="trpC"
                     /locus_tag="VW144_001705"
     CDS             5094..5861
                     /gene="trpC"
                     /locus_tag="VW144_001705"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU9601120.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            5848..6447
                     /locus_tag="VW144_001706"
     CDS             5848..6447
                     /locus_tag="VW144_001706"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU9601121.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQSFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKDGISGTK"
     gene            6425..7648
                     /gene="trpB"
                     /locus_tag="VW144_001707"
     CDS             6425..7648
                     /gene="trpB"
                     /locus_tag="VW144_001707"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU9601122.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGRDLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            7641..8417
                     /locus_tag="VW144_001708"
     CDS             7641..8417
                     /locus_tag="VW144_001708"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU9601123.1"
                     /translation="MPKTLTEKLNAIKAAGKGIFVPYIMAGDHEKGLDGLGETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIKTEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDITLIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(8389..8490)
                     /locus_tag="VW144_001709"
                     /pseudo
     CDS             complement(8389..8490)
                     /locus_tag="VW144_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            8719..9450
                     /locus_tag="VW144_001710"
     CDS             8719..9450
                     /locus_tag="VW144_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601124.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            9465..10616
                     /locus_tag="VW144_001711"
     CDS             9465..10616
                     /locus_tag="VW144_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU9601125.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNITIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(10668..11327)
                     /locus_tag="VW144_001712"
     CDS             complement(10668..11327)
                     /locus_tag="VW144_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU9601126.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTPLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELGLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            11397..11843
                     /locus_tag="VW144_001713"
     CDS             11397..11843
                     /locus_tag="VW144_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9601127.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLPLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSGLKLE"
BASE COUNT         3752 a         2080 c         2737 g         3319 t
ORIGIN      
        1 taatggagga ctgaaatgaa aaatataaat gggaaaaagg taactattta tgatattgca
       61 cgtttatcag gtttttctcc aaaaacagtt tcacgagtta ttaatggtgg agtaaatgtt
      121 aaagaagaaa cttatcaagc gattcaaaaa gttatagagg aattgtcgta tattccaaat
      181 gcgtatgcaa aaaatttaac taaaaaagaa gctataaata tattgatttc tgtaaaaaaa
      241 atcgactctt ttcctttaat ttggtttcat acgctgttag ataaagtgtt acggacgtgt
      301 aaagaatttg gtgttaatgc tatagttgag tactttggag aggaggatac aattagtaat
      361 tcaattatat caagcacagg tagcctagta gatggtgtta ttgtttttta tgaaagtgta
      421 gatgatatca gaattcagta tttaaagaag aaccatatgc cttttcttgt tttcggtgaa
      481 tctcaaacgt ctggagtagt ctatgtatct aataataatt ttcaagctac ttatgatatg
      541 atgaaagctg taactgaaga aaaatttaaa aatatgtggt tgcttatggg aggagaatcc
      601 catgttaata aggatcgtga aagaggtgtg cgttcttttc tgaatgataa aaattacttt
      661 atggatttaa aagtcattta tggcttatct acaattgatt cagtttattc ttatgcaatg
      721 cagcacttga ctacccaaaa ttatccggac attatttttg tttcaggaga tgaaaaggtt
      781 cagggcttga ttcgtgcgtg ctatgagaaa ggaattctta ttccagatga tatttctatc
      841 attggatttg ataatattcc tatttctcag tattatactc cagctttgtc tacaattgct
      901 cccaattatg ttaaattggc taaagaaatg atagaaggtg ttttagcaat tattaaaggg
      961 gaaagtgtca catctgttga agtttctcct aaatttgtta ggagacagag tttctagtat
     1021 tgtttaattc ttgaaaatcc tagtggaaaa gatggagtgt tctaaaataa gttctgttta
     1081 gcctccattc agtttctatt gagttggatc caatttctaa caagtgggac atgttcatgt
     1141 tcgatctaag gaagacatgg tcacattcga atgatatttt caaaaaaatc gtaaaaaagt
     1201 cttgacaaag aaaaaacaaa gtattaaaat aagttcaaca aatcagaaaa gtaactattt
     1261 ttgatcttca gggagcctgt ggtgattgtg aataggtggt tggaagtagt gaaagtgggc
     1321 tgattttgaa aatgaatttg aaacaatgaa aattcggtgc gcacacctta cagtgcagct
     1381 tgttgttaaa caaggcagag atgtaaaggg atagtccctt tataattgag gtggcaccgc
     1441 gttaccaacg ccctcacacg gaagtatatt ctgtgtgtgg gcttttttct atccgtcgtt
     1501 tggtttatct tttattaggg ccttaagtcg ctttgatgaa cttgagttct atctacagct
     1561 ccttgcctac tactaaaagc aaacaaaaag gctggactaa tacgaaaaaa gctagtaata
     1621 ctcaatgaaa atcaaagagc aaactaggaa actagccgca ggctgctcaa aacactgttt
     1681 tgaggttgtg gatagaactg acgaagtcag taaccatacc tacggcaagg cgacgttgac
     1741 gcggtttgaa gagattttcg aagagtataa aggtgtaccg agatgaacat ctcttcatca
     1801 atgtctctgg tcgtggaggc aaggacctag ctgcgattgc agattactag aagctaaaaa
     1861 ataatagatg gaaaaattac agtcttttct ctcacagaga gcttgtggtt gctgaaaaca
     1921 agcagagaaa gctgtaaagt tgggttcatc tgaaacgaga gaagaaaaca tcattctcaa
     1981 gggagtgccc tttatcgcac agcctgttac aggaggtatc tgagacagga atgagagata
     2041 ggagaaatcc tataattgag gtggcaccgc gaatttcgtc ctcacgcaag ttattttgcg
     2101 tggggatttt tcatacagtc gccacgaact agaattagaa ctgaaaggga aattacaatg
     2161 gaacgaatca ttcatggaga tgtcttatca ccaatcttgg cttatatgcg cttaaagggg
     2221 caacataagg ttattttaga gagtattccg agagacaagg aaacggctcg tttttctatc
     2281 ctagcctata atccagtttt tgagattaag tttgaaaatg gagttctcta tcaaaatggt
     2341 caagtgattg atcgggatcc cttggatttc ctttatgaag tgattcataa gagtcagcac
     2401 cactcagagc tcccttttgg tggtggagcc attggttttg tgggttacga tatgatttcg
     2461 ctttatgaag aaattggtca aatccctgag gatacaattg ggacgccaga catgcatttc
     2521 tttgtctatg agagctatat ggtctttgac cacaagaagg agaagataca tgtcatagag
     2581 gatgcacttt atagtgagcg cagtcaagag gccttggaaa aatcattgaa ccaagtgctt
     2641 gaggaattac gcattcctgt tccaaatgaa tttgaagact tggatctatc tccgttagac
     2701 ttcaaaccgc atatcgctcc acataagttt gagggaatgg tagaaacagc tcgtgacttg
     2761 attcgtaacg gagatatgtt ccaatgtgta ctcagccagc gtttctcagc agaagttact
     2821 ggaaatccat ttgacttcta cagaaatctc cgcgtgacta atccttctga ttacctttat
     2881 ttctatgatt ttggggatta tcaaatcatc ggagctagtc cagaaagttt ggtttctgtc
     2941 aaaaatggca tcgtgacaac caatccgatt gcagggacgc gaccaagagg ggctacggat
     3001 gaagaagaca agaccttggc gactgacctc ctctctgatg agaaggaaac agcagaacat
     3061 cgaatgttgg tagacttggg gcgtaacgat attggccgca tctctgaaac ggccagtgtc
     3121 caagttacta agtatatgaa ggtggagctc ttccgctacg tcatgcattt gaccagtgtg
     3181 gtcaaggggc gtttgcttgc agaactcact gctatggatg ccttgaaagc aacgcttcct
     3241 gctggaaccg tttctggagc accaaaaatt cgagcgatga gacgcatcta tgaactggaa
     3301 acggaaaaac ggggcgtata tgcaggagca atcggctact tgtctgcgac gggtgatatg
     3361 gatttggcca ttgccatccg aaccatgatt ctcaaaaatc aaagagccta tgtgcaggct
     3421 ggggcaggga ttgtctacga ctctatcgcc caaaacgaat accaagaaac cattaacaaa
     3481 gcaaaatcta tgactagaat tggagaacta agaccatgat tttattgatt gacaactatg
     3541 attcttttac ctataacttg gcccaataca ttgggaattt tgcagaagtt caggttttga
     3601 gaaatgatga ttccaagctg tatgaagaag ctgaaaaagc agatggtctg gtcttttctc
     3661 ctggtcctgg ttggccagtt gatgctggaa agatggaaga catgattcgt gattttgcag
     3721 gcaagaagcc gattctaggg atttgtttgg gccaccaagc cattgcagaa gtctttggtg
     3781 gtaagctagg tttggctcca aaagtcatgc atgggaaaca gagcaatatc aactttgaag
     3841 cgccatctgt tttgtatcaa ggtattgagg atggccgtgc ggtcatgcgt tatcacagta
     3901 ttttgattga ggaaatgcca gaagactttg aagtgacagc tcgttcgact gatgaccaag
     3961 ccatcatggg gattcaacat aaaaacctac cgatttatgg cttccagtac catccagaga
     4021 gcattggaac gccagacggc ttgtcttcta ttcggaattt tatcgaggag gttgtaaagt
     4081 gaggaaacta ggatgaaaga gattattgaa aaactagcaa aatttgaaaa tttatcaggt
     4141 gtggaaatga cggatgtcat tgagcgtatc gtaactgggc gtgtaactga agcgcagatt
     4201 gcttctctcc tcttagctct taagatgaag ggggaaacac ctgaagaacg cacagccatt
     4261 gcccaagtca tgagaggaca tgcccagcat attccaactg agattcatga tgccatggat
     4321 aactgtggta caggtgggga caagtctttc agttttaata tttccacaac tgcagccttt
     4381 gtcttggctg gtggcggtat tcacatggcc aagcatggta accgctcgat ttcttctaaa
     4441 tctggttccg cagatgtcct cgaagccttg ggaatcaatc ttgatctcaa accagctgaa
     4501 ctaggtaagg tctttgataa aactggaatc gtctttctct tcgctaaaaa tatgcaccca
     4561 gctatgaaat acatcatgcc agctcgtttg gaattgggaa ttccaacgat tatgaacttg
     4621 actggtccac tgattcatcc aatggcttta gaaacacagc ttcttggaat tagtcgtcca
     4681 gaactcctag aaagtacagc tcaggttttg aaaaatctgg gtcgcaaacg tgccatcgtg
     4741 gttgctggac cagaagggtt ggatgaagct ggcttgaacg gaacaaccaa gattgcactt
     4801 cttgaaaatg gcgaaatcag cttgtcaagc tttactccag aggatttggg aatggaaggc
     4861 tatgctatgg aagatattcg tggtgggaat gctcaggaaa atgcagaaat tttgcttagc
     4921 gttctgaaaa acgaagcaag tccattcttg gaaacgacag tcttgaatgc tggtcttggt
     4981 ttctatgcta atggtaagat tgatagcatc aaggaaggag ttgccttggc ccgtcaagtg
     5041 attgctagag gcaaggccct tgaaaaactc agactgttac aggagtacca aaaatgagtc
     5101 aggaattttt agcacgaatc ttagagcaga aggcgcgtga ggtggagcag atgaagctgg
     5161 agcaaatcca gcctctgcgc cagacctatc gcttggcaga atttttgaag aatcatcagg
     5221 accgcttgca ggtaatcgct gaggtcaaga aagctagccc tagtttggga gatatcaatc
     5281 tcgatgtgga tattgtgcaa caggcccaga cttatgaaga aaacggagca gtgatgattt
     5341 cggtgttgac agatgaggtt ttctttaaag ggcatttgga ttatctacgg gaaatttcca
     5401 gtcaggtaga gattccgacg ctcaacaaag actttatcat agatgaaaag caaatcatcc
     5461 gcgctcgcaa tgcaggtgcg acagttatct tgcttattgt ggcagccttg tccgaagaac
     5521 gcctcaagga actgtatgac tacgcgacag agcttggtct ggaagtctta gtggagactc
     5581 acaatctagc tgaactagag gtagcccaca gacttggtgc tgagattatc ggggtcaaca
     5641 accgcaactt gactaccttt gaggtcgact tgcagaccag tgtagattta gccccttact
     5701 ttgaggaagg tcgctattac atttctgaat ctgccatttt cacagggcag gatgcggaac
     5761 gactagcccc atactttaac ggaattttgg tagggacagc tcttatgcag gcagaaaatg
     5821 tggcccagag aatcaaggag ttgcagattg acaaaggtta aaatttgtgg actatcgacc
     5881 aaagaagcgg tggaaacagc cgtttcagca ggagccgact atatcggttt tgtctttgca
     5941 cctagtaaaa gacaggtgac tttagaagag gcagctgagt tggcaaagct tattcctgca
     6001 gatgtgaaaa aggttggagt atttgtttca ccaagtcggg tagaactgct ggaagcgatt
     6061 gacaaagttg gcttggactt ggttcaagtt cacggtcagg tggcagatga tttatttgag
     6121 aatttgcctt gtgccagtat tcaggctgtg caggtagatg gaaatgggca tgtccccaat
     6181 tctcaggcag attatctact ctttgatgcc cctgtggcag gaagtggcca gtcctttgat
     6241 tggggtcaac tggatacgac tggactagca cagtccttct ttatcgcagg tggccttaat
     6301 gaagataatg tagtaaaagc aattcaacat tttactccct atgcagtaga tgtatcgagt
     6361 ggagtggaga cagatggaca aaaagatcat gaaaagatta gaagatttat agagagggta
     6421 aaggatggca tatcaggaac caaataaaga tggattttac ggaaaattcg gcggacgttt
     6481 tgtcccagaa acattgatga cagcagtttt ggagttggag aaggcctacc gtgaaagtca
     6541 ggcagaccca agtttccaag aggaattaaa ccaacttttg cgccagtatg tgggacgtga
     6601 aactcccctt tactacgcaa aaaatttgac ccagcatatc ggcggagcca agatttatct
     6661 caaacgggaa gaccttaacc atacaggagc ccacaagatt aacaatgcct taggacaagt
     6721 ttggcttgcc aaacgcatgg gtaaaaagaa aattatcgca gaaacgggtg ctggtcagca
     6781 cggtgtggca actgcaactg ctgcggccct ctttaacatg gaatgtacca tctacatggg
     6841 tgaggaagat gtcaaacgcc aagccctcaa tgtcttccgt atggagcttt tgggagctaa
     6901 ggtcgaggcc gtgacagatg gttcgcgcgt gctcaaggat gcggtcaatg cagcccttcg
     6961 ttcatgggtg gctaatatcg acgataccca ctatatcctt ggttctgcct tggggcctca
     7021 tccatttcca gaaatcgttc gtgacttcca aagtgtcatc ggtcgagagg ctaaacaaca
     7081 gtaccgtgac ttgacaggtc gagatctgcc agatgcccta gtagcctgtg ttggtggtgg
     7141 atctaatgct attgggctct tccatccctt tgtagaagat gagtcagtag ccatgtatgg
     7201 agctgaagcg gctgggcttg gtgtggatac agagcaccac gctgctacct tgaccaaggg
     7261 tcgtccgggt gttcttcacg gttctctcat ggatgtgctc caagatgccc atggtcaaat
     7321 tcttgaagcc ttctctatct cagcaggttt ggactatcct ggtatcggtc cagagcattc
     7381 tcactaccac gatatcaaac gtgccagcta tgtccctgtg acagacgaag aagctttgga
     7441 aggatttcaa ctcttgtctc gtgtggaagg gattatccca gccttggaat ctagccatgc
     7501 tatcgctttt gcggtgaaat tggccaaaga acttggacca gaaaagtcta tgattgtatg
     7561 cctatcaggt cgtggggaca aggatgtggt tcaagtcaaa gaccgcttgg aagcagatgc
     7621 agcaaagaag ggagaagctc atgcctaaga cactaacaga aaaattaaat gctataaaag
     7681 cagctggaaa aggaattttt gttccctata tcatggctgg agaccacgag aaaggtttgg
     7741 atggactagg agaaacaatc cactttttag aagatttggg tgtctctgca attgaagtgg
     7801 gtattccctt ttcagaccct gttgcagatg gacctgttat cgaagaagct ggcttgcgca
     7861 gtttagccca cgggacctct acccaggctt tagttgaaac cttgaaaacc attaaaacag
     7921 agattccact ggtcatcatg acctacttca accccctctt tcagtacggt gtggagaact
     7981 ttgtcaaaga tctggctgat acagctgtga agggcttgat tatcccagac ttgcctcatg
     8041 aacatgccaa ctttgtagaa ccatttttgg cagatacaga tatcaccttg attcctttag
     8101 taagcttgac cacaggaatt gagcgccaga aagagttgat tgaaggggcg gagggattcg
     8161 tctatgccgt tgccatcaat ggagtgacag ggaaatcagg caattaccgt gcagatttgg
     8221 acaagcactt ggcgcaactt catcaagtgg ccgacatccc agtcttgaca ggttttggtg
     8281 tatctagtca agccgatctg gaacgcttca atgcggtgtc agatggtgtt atcgtcggtt
     8341 cgaaaatagt aaaagctctc caccaaggag agccgattca ggactttatc aaacaagcag
     8401 tagcttacca aaaataatca aacaagtagc aagtaagagg aaaggaacga aaggaagtct
     8461 ttcctttttc ttttgcagga gaaaggctag ttttatagta aattgaatct ggaatattac
     8521 actacggttc ctaaaatatt tttagaaagt gatttgaatt tcccaataaa tttgttcagt
     8581 ttttaattga ttttactata gaagatataa ttttctaaat tactctgaca cagaacttta
     8641 agaaaaagct aattattgta gtaatttctt taagaaaatg atataattca ttaaaaagta
     8701 tttggaggag gaagaattat gttgaatcag gatctctttg attcgcttga ggcacaaaaa
     8761 attgtagata ctttgatgaa agggcaaaaa gattatgtag atgaacgtct agaaaaaaga
     8821 gagacaatga tagtgtcgaa tggttatgca tggacgcgac ctaatcatat tgatactgca
     8881 tttgcatcag cagatttgtt tgagtataaa ttacaattag caggacagac ttggggatat
     8941 ttagaatttg aaacaaacac agaaaaatat gggaaagtat tgttaattat aaagggtaag
     9001 aagcgactta cgaaccaatt tcctttggta caaaaaaata agagtggcta cttatttgaa
     9061 tatgctcaga tgaatacact ttatcttaat caacattctt cctacaaaaa tgatgaagat
     9121 agtcattcct ttccaattca gatggagtta gtttctgatg aaatgattca agaaattgaa
     9181 caagctacta aaaattcgaa tatcgaaaaa tttatgattt taacttatga ggcggactca
     9241 gaaaacaata ttatatctgt agatgttgtt atgcctgatg cacgaactgg tcagttacac
     9301 ttgattcaag atttgtctga gtatattcaa tcaagttcgt accatttcga agaagctaaa
     9361 tatcaagata ttcctaattt ttcagaatta tcggaaacag aagattttga aattattcca
     9421 agaatagaaa aacaagaagg tcaaaagtag taaggagtat gtaaatgttt aatggtcggg
     9481 tattgaaaga attacggctg ttaaatggtt taagtagagc agaattagct cagagaatta
     9541 atttaacgga acaagccatt tggcagtttg agtccaacga aacgaaacct aaattatcaa
     9601 ccaaaatgca tttggccaac caatttcatg ttgatttaac ttattttgaa caggaagaag
     9661 agagcattcg atttgattct tctgtaattg cctttagaaa tgcagaccta gcaacccgga
     9721 aaacaataga tattcaaact atgtatttac ataaggtaga tagtttgatt gattattttg
     9781 aaagttttgt aattatacct aatattacaa ttcatgacct aagtaatgta gtgagtgaat
     9841 cttatcataa gggagaatcc attgaggaat tggctcttta tgccagggaa aaattaggta
     9901 tttcaaaaga taatcatgat ttgctttata aattagaacg ttcaggcatc tatatcgtgg
     9961 aacgattaat taatggccaa gctgatgctt atagcgcatg gtcaaaattg ggaagacctt
    10021 atattgtgtt gggaacgaat aaatcatctg tacgtcgaaa ttttgattta gctcatgagc
    10081 taggacatat tcttttacat aaatataaag atatgaatga agatggcgat cgtttggagc
    10141 aagaagcaaa ttattttgca tcatgttttt tattgccaaa agaagagttt ttagtcaaat
    10201 ttgaagagag ggttggcaag cgtgtcagca atcctgatag ttatatttta ttgaagtcgg
    10261 atttgaatgt ttcgatacag gctttagagt atcgagcttt taagttagga ttattgactc
    10321 caaagcaaca ttcttacttt tatcgtcaaa ttgcgcaaaa aggttacaaa atgattgaac
    10381 ctttggatga tcaaattttt gttaaaaaac caagcaaagt aaagagtatt ctggacgtcg
    10441 ttttgagtaa tcatctagtc agtctagcga ctataatgtc taaacaaagt attcgtttac
    10501 agtttataag cgaaatattt tcagtcgaaa tgaaattttt tgatcagtat caagaagata
    10561 gaagaacaga tcgatttgat aacatcatcc ctttgtacaa aagaaataat ttataattta
    10621 actgtgaaat aatttcatgg agtacatatg gcagaaattg gaatttatca aacaagtagt
    10681 agcttaccaa aaataatcaa acaagtagca agtaagagga aaggcacgaa aggaagtctt
    10741 tcctttttct tttgcaggag aaaggccagg atacccgtcg cagaagcgaa ctgaatcaag
    10801 atcagtaact ccgttacgct aaagacgaga gcacaagaag ctaaaaagag gaaatcccct
    10861 gcacccatgc ggatatcaat aaaatgagcc aaaattccaa gtatgaggaa ggagaccatg
    10921 accagattcc agccagagga agctattagg attaggtgga aagtcatcca gaccagtaag
    10981 ggatattcct gatggtgaaa gtcgtatata cccaaggtca aaccagcggt gattaggacg
    11041 acttgcccca aggaaagcaa ttcccaagag taaagcagaa agaggagtcc taagcctagt
    11101 tcaaagaggg cataccagac aggatagcga actttgcagt agcgacagcg aaagcgattg
    11161 aagacctgtg agagaatcgg aatcaaatct aagggacgca agggagtctg acaggaatcg
    11221 cagtgactgg ctgaactgat aatggattgc tctggaaaac ggtcaatgac caaaccaaga
    11281 aaggaagcta gaatgctccc gacgagaaaa aaataaaaat caatcatact tatctattcg
    11341 taaaaaatgg gagaaatagt ataatggaga aacatagata agatggtgag gaaaagatga
    11401 caattcgttt tgaagaaaag gtgagtatag aaaacgctca atttgtatgc cagtggtcca
    11461 actccctagg caaatccttt caagaacaat ggatgggacc aaggattcct tttctactgc
    11521 cccttcaagc cttggaagga gtcttttcaa tctttgatga acaagagttt gtgggactta
    11581 tccagaaaat caggctagaa gacagcaatc ttcatatcgg gagatttttt atcaaccccc
    11641 agaaacagga gcaagactta ggtagccagg ctttaaggaa atttgttagt ttggcctttg
    11701 aaaatgaaga tatagatagt atttctctaa atgtcttcga ggcaaatcaa agagctcaga
    11761 atctttacca aaaagaagga tttgaaatcg ttcaaatggt tgaagcacct gtacgaaaat
    11821 atagtggatt gaaactagaa tagtacacct ctatttctaa aacattgtta gaaatcgatt
    11881 tgactgtc
//
LOCUS       DAVKJJ010000056        11590 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000056 DAVKJJ010000000
VERSION     DAVKJJ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11590
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.056"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            <1..166
                     /locus_tag="VW144_001714"
     CDS             <1..166
                     /locus_tag="VW144_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601128.1"
                     /translation="NKELGIDLSIEIDKLIKQGEKPSVIAQIKKFQDQVNKDNEYREM
                     MKNKKMDQER"
     gene            240..413
                     /locus_tag="VW144_001715"
     CDS             240..413
                     /locus_tag="VW144_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601129.1"
                     /translation="MNKRQELIDELIKANEDGTYKTYKSTEEIKAMNNEEIQIIYSNM
                     KNYLSDKRTHINY"
     gene            599..1126
                     /locus_tag="VW144_001716"
     CDS             599..1126
                     /locus_tag="VW144_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HEU9601130.1"
                     /translation="MINEEISKEAGQAAQTIISYTIKATKESINLEKEIRKKMNETLE
                     KANGNLKSLMGDEMKIKDLYKKGQLENISIDQIDLKDLKKELNKLGVSFSVMKNKESK
                     NYEIFFQAKDIKVMEYAFKQVIAKENKKEKESILKQIKKYKDLSKNKDKTKEKGKRKV
                     KEKVKPNKKDMTREI"
     gene            1253..1981
                     /locus_tag="VW144_001717"
     CDS             1253..1981
                     /locus_tag="VW144_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEU9601131.1"
                     /translation="MATNKRYYWIKLKEEFFTDKRIKRLRRISGGDTYTIIYLKLLLL
                     SLKDEGKLYYDGVESDFIKELALTIDETDDDVMVTVNYLINQGLLEVVTENDEYYLTE
                     IPNLIGSETAWAEKKRRYRQNKQRTLSLMSPTHVRQEIEIEKDIEIDKEREGEIDKKS
                     TPIFYGEFKNVRLSKEEYKNLKEKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWY
                     EEDKDKLRQKGLNKKMNYDVGESL"
     gene            2665..2826
                     /locus_tag="VW144_001718"
     CDS             2665..2826
                     /locus_tag="VW144_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601132.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            2918..4597
                     /locus_tag="VW144_001719"
     CDS             2918..4597
                     /locus_tag="VW144_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU9601133.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGRIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGYKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKIDELESSLIDAKAKKQTIEAEKPTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            4865..5431
                     /locus_tag="VW144_001720"
     CDS             4865..5431
                     /locus_tag="VW144_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601134.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            5752..5940
                     /locus_tag="VW144_001721"
     CDS             5752..5940
                     /locus_tag="VW144_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEU9601135.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            6002..6760
                     /locus_tag="VW144_001722"
     CDS             6002..6760
                     /locus_tag="VW144_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9601136.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            6818..7672
                     /locus_tag="VW144_001723"
     CDS             6818..7672
                     /locus_tag="VW144_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU9601137.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            7784..8668
                     /locus_tag="VW144_001724"
     CDS             7784..8668
                     /locus_tag="VW144_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEU9601138.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            8831..9958
                     /locus_tag="VW144_001725"
     CDS             8831..9958
                     /locus_tag="VW144_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HEU9601139.1"
                     /translation="MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEI
                     DRHFGDKETFRELVDQAHHRGMKVMLDAVFNHIGSQSLQWKNVVKNGEQSAYKDWFHI
                     QQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYWIEEFNIDAWR
                     LDVANEIDHQFWKDFRKAVLAKNPDLFEIIKNIKNLVINLN"
     gene            9907..11243
                     /locus_tag="VW144_001726"
                     /pseudo
     CDS             9907..11243
                     /locus_tag="VW144_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261806.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(11284..>11590)
                     /locus_tag="VW144_001727"
     CDS             complement(11284..>11590)
                     /locus_tag="VW144_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="HEU9601140.1"
                     /translation="NKTNKRIVTQLAINCFILSIDKEEFSNCSYLINEIKGLLENELN
                     FYEQTVFLYATGYYEFKRQLSSGIETMKQAIQVLDILGEDKLKLHYTSHFDKLVNNK"
BASE COUNT         4455 a         1592 c         2282 g         3261 t
ORIGIN      
        1 aaacaaagaa cttggtatag atttatccat agaaatagat aagctaataa aacagggaga
       61 aaaaccaagt gtaatagcac agattaaaaa gttccaagac caagtcaata aagataatga
      121 atacagagaa atgatgaaaa acaagaaaat ggatcaagaa agataagacc tggtaaaaat
      181 atcttatcca agctataata tgaccaaaca actaaagtca gatctaggag gtaatagaga
      241 tgaataaaag gcaagagcta atagatgaac tcataaaagc aaatgaagat ggaacataca
      301 aaacctataa aagcacagaa gaaataaaag caatgaacaa tgaagaaata cagattatat
      361 attctaacat gaaaaactat ctatcagaca aaagaacaca cataaactat taaaggtgga
      421 aaggtagtgt aagctatttt taaaagtata aaaggtacaa agacctaaga aatatataaa
      481 aacatcaaaa taaagtattc caccaccaag aacaataaaa taaaaacaag caaagggaag
      541 tatagaaaaa ttaaaaccta tacttccctt ttttaattca aacaaaggag agaaaagcat
      601 gataaacgaa gaaataagca aggaagcagg tcaagcagca caaaccataa tatcatacac
      661 aataaaggca acaaaagaat caatcaattt agaaaaagaa ataagaaaaa agatgaatga
      721 aactttagaa aaagcaaatg gaaacttaaa aagtcttatg ggcgatgaaa tgaaaataaa
      781 agacctctac aagaaaggac aactagaaaa tataagcata gatcaaatcg acctcaaaga
      841 cttaaaaaaa gaactaaaca aacttggagt aagtttctca gtaatgaaaa acaaagaaag
      901 caaaaactat gaaatattct tccaagccaa agacataaaa gtaatggaat atgcctttaa
      961 gcaagtcata gccaaggaaa ataaaaaaga aaaagaaagt atcctaaaac aaataaagaa
     1021 atacaaagac ctatccaaaa acaaagataa gacaaaagaa aaaggaaaaa ggaaagtaaa
     1081 agaaaaagta aaaccaaaca aaaaagatat gaccagagaa atctaaaggg agtaacgaaa
     1141 agttacaccc ttaaataacc ataataacaa gacttccgaa aaacagaagt ccagaattcc
     1201 gaaaatcgga aatcaagaat ttcgattatc gaaaatcaag aaaggagcag atatggcaac
     1261 aaacaaaaga tactattgga taaaattaaa agaagaattt ttcacagaca aaaggataaa
     1321 aaggttaagg agaatatctg gaggagatac ctatacgata atctacctaa aacttctact
     1381 actaagccta aaagatgagg gcaaactcta ttatgacgga gtagaaagtg attttataaa
     1441 agagttagca ctaaccatag atgaaacaga cgatgatgta atggtaacag taaattattt
     1501 aatcaatcaa ggcttattag aagttgtaac agaaaatgat gaatactacc taactgaaat
     1561 accaaactta atcggatcag aaacagcctg ggcagagaaa aaaagaagat acagacaaaa
     1621 taaacagagg acattgtcct taatgagtcc aacccatgtc cgacaagaga tagagataga
     1681 gaaagatata gagatagata aagagagaga gggagagata gataaaaagt ccacccccat
     1741 tttttatgga gaattcaaaa atgtaaggtt aagcaaagaa gaatataaaa atctaaaaga
     1801 aaaattaaac tcacacacag atataatgat aaataaacta tccagataca tggaaagcag
     1861 tggtaagacc tatcaaaacc actatgtgac aatcttaaaa tggtatgaag aagataaaga
     1921 caaactaaga cagaaaggtt taaataaaaa aatgaattat gatgtaggag aatctttata
     1981 gataaaaaga ggtggaaagg cactattaaa gactttaagt ttaaaaaggt atcaaagtat
     2041 gccagagata aaaaaatata aatatgacac tttaaaggcg attagaaaat taaagaaatg
     2101 ctagaagact tttagatgac agttaccaag agggtctaat tttcactgct acttatgcag
     2161 taggaattgg acctttttat ataagcaaat tctatttcaa aattggttac caagaaagac
     2221 ttcttttcac tgttaagtat aagagggtaa tttatagaat tttgttacat caagataaat
     2281 attttctgat ggtcaaattt taaaaagatt aataactctc gataaatttt gaaccttaac
     2341 aattgaatag accaagacaa gacgttaatt atttttggag cgtaataacc tatccgtcaa
     2401 gatctttcgg ctgaataatt cggcaaaaaa agttctgcta agcaaaaaga aagtgagcga
     2461 taagtaagag tttgaatata gggagggata ccctatccgc tatgaagaaa atgaggataa
     2521 tgatacttct aaggttgaag caggctcatc cggaatagag gcggaggtga gattcctatg
     2581 ttgctaatcc aaagcacttg tcaatgtcaa aaaacttaat taaacaaata attattagat
     2641 ataaaacttt agaggaggta tatattggac aatgactgga atggattggc tgatttaata
     2701 gcaaatctga ttgcaaaata tgctggtgct ttagatttag ataatctacc cgatccaacg
     2761 ccagcaaaaa atcaagagat gaaaaatagt tttgacatgg caaaaacaca aattgagacg
     2821 gactaataaa agtgttataa tatacttgaa atgatagtcc aaacttaaat taaaaaaatt
     2881 atatatgata tagaaaaatt atattgaggt taatactatg tcaaaagaaa aaataaaagt
     2941 atacctctat acacgagtat ctacgtcaat acagatagag gggtattcgt tagaggcaca
     3001 aaaatcacga atgaaagctt ttgctattta caatgattat gaaattgttg gagaatatga
     3061 agatgccggg aagtctggca agtctattga aggtagaata cagttcaatc gaatgatgga
     3121 agatataaaa tctggaaaag atggagtatc ttttgttctt gtgtttaagt tatcaagatt
     3181 tgcaagaaat gccgctgatg ttctttctac acttcagata atgcaggatt atggagttaa
     3241 tcttatctgc gttgaagatg gcattgattc atctaaagat gctgggaaac taatgatttc
     3301 tgttttatca gctgtggctg aaatagaaag agaaaacatc cgtattcaaa caatggaagg
     3361 tcgcattcaa aaagcaaggg aaggaaaatg gaatggtggc tttgctccgt atggatataa
     3421 acttgaagac ggcaagctgt ttataaatga ggaagaggca gttgcaataa gaacgatttt
     3481 cgaccagtat gtaaatacta cgataggagc caatgggata tctaaatact tagagaatca
     3541 tggaattaga aaaatcccaa gacagaatgg taagaatcct ttgtttgatg caggtcttat
     3601 aagaaagata ttaaagaatc ctgtatataa tgggaaaata gcatttggaa gaagaacttt
     3661 agaaaaagtt catggtacaa gaaatgaata taagcaggtt gaacaagatg aatatctaat
     3721 atctgaaggg atacatgaag ctatagtttc cgatgaagtt tggcaagctg ctcaggttaa
     3781 gctaaaatct caagcaaaga aatatgagca tgtgaataaa ggaaaagata cacgcacaca
     3841 cttgctttct ggaattgtaa aatgcccgat atgtggagtg ggaatgtttg ggaacaagtg
     3901 tatcaagaaa aagaaagatg gcacaaagta taaagatttt tattactatg gttataaaca
     3961 taggcagatg ataagaggtc ataagtgtac attcagtaag caaattagag aagaattgtt
     4021 agatgatgct gttgcagagg tgattgtcaa gatagtaagt aatccgaaat ttgcttctat
     4081 gatgcaagaa aaaatcaaca tgaaggtgga tacctctgaa atagaaaaag aaatagataa
     4141 ttaccaaaag gaattgagaa agagtcattc tacaaagttt aagctaattg aggaaataga
     4201 taatttagat gttgaagata agcattataa gagaaggaaa caggatttag atgatagact
     4261 atatcgtatg tatgacaaaa tagatgaatt agaatcatca ctaattgatg cgaaagcaaa
     4321 gaaacagact attgaagctg aaaagcctac aggagataac atatataagg ttctgatcta
     4381 ttttgataag ctctataaag tcatgaatga tgtagagcgt aggcagttaa tttcagcttt
     4441 gatttctgaa attcaagttt atgaagaaaa acaatctaac ggacaatggc taaaatccat
     4501 tacttttaag ctaccaatta tagaagaaaa tctcaacata ggtttggata atgatgaaca
     4561 agttgaatgt gtaagtttat tagagaaacg tagttaatcc tcaaaaggtt ccccaaacct
     4621 tttgagtccc actgacgcat cacgctgagg cggtatcact gcttgtacga gttgaagcat
     4681 cagcgaagta aaagtagatg ttcagccaat gcgacgaggt gagaccgaag gtcgataagg
     4741 tgtgttctgg gtataggact cgtaaagtaa gtaggaaata tgatgagatg atacagagag
     4801 cctgaattgg tcaggtgacg tgcttattta cattagcctg ataatgaaaa tggagagatt
     4861 atcgatggtt atattggaat tgtaccaaaa tgattattct aaagatttag tagcatttga
     4921 ttccatagaa gatgggaaag cctttgtagc tcaaatcccg ggatacactc tggaaacaga
     4981 agatggtttt gaagtggagt attttaatcc taaaaacata ccggattata tggaaattat
     5041 ttttaacgga aatattgttc ctttatctaa atttatgttc gatcctgagg aaaatgtaaa
     5101 cattatttgg aaagagattt caaatttatc cctcaaaaat gatagagtga tcgaaggata
     5161 ttcaaaaatt gatgcatatg tcgttaacaa ccatgaagtt aaagcctatg tcgaaacaag
     5221 agaaacgaac tatcgtaaag caaaagactt cttagaaagc cgcggatatg aaatcgacag
     5281 aagctttttc ggaagtgaag acggtgaagc gattctttac agaaaaaaag ggatcgaagt
     5341 ttggcatttc ttgtgtcact tagacccaat gtttgtagag attgaagatg tagaaggata
     5401 tgttaaagaa gaagttggag agattcaata gtcaaccaaa gttccttgtg agtatatacc
     5461 tttgctagta aaagagtcaa aacgttataa cgaagggggg aagaaaaccc ttgagtgtaa
     5521 gtctttttct tgtttagagg gaaagtatta gagtagccgc tcataattta tactagattt
     5581 gataactgat ttgagacaga ttttatttct ataaatattg tgaaaatttt ttaataccaa
     5641 agatttaaag ttactgtgtt aaaaatcctc tccactttta aactaatgta ttataaaagc
     5701 cagttgacac aagtgttaca cctatgatag aattaacttg aggtgatgaa aatggcaacc
     5761 gataaaaata gaataatgat tagcttggat gataaaaatc tagagaaact tgaaaattta
     5821 gtagaagatg ctagagatag aaggggaatg cgactaacaa agtcccaggt tattgaatta
     5881 ctcctaaata ccgttgatta tttcgatgat attatggggg ctatttactc aaaaaaataa
     5941 gagcaaaatt gctattattt tttacaaatt ggtgtaacac tagtgatata ggagtgatga
     6001 aatggttgtt tatattagac aaagtaaatt accaagtgag gtttcaatta ataaatacaa
     6061 tgctcaagta ggtgcctact tacaaggaga agaagttatt ttatatcaat ctttttctga
     6121 aataaaagag ttaacaagtg aagatatagt tgtagattat ataatggaga ctagagcatt
     6181 actaaaaatg atgggcttaa acgttccggt tcatgactat cctattgagc ttaaagagtt
     6241 ttatggtcga aagatttatg ctggtatttt aggagagatt gtgaatatac ctgataattg
     6301 gggaaaattt attaagccta aagctggttc aaaagtcttt actggaagag ttgttaatgg
     6361 aacccatgat ttaataggta ttggtctacc tttcgactat cctatatgga ttagtgaggt
     6421 tgtagaattc atagctgaat ggcgttgttt tgtgttagat ggtcgcgtat tagatgttcg
     6481 accctataca ggtgattatc atgcacaatt tgatgcaagt gtaattgatg aagcgatatc
     6541 atgttggaaa gatgcgccaa tagcttatgg actagatatc ggtgttactc gcgatggcag
     6601 aacacttgtt gttgaagtaa atgatggtta tgcattggga aattatggcc tatctccttt
     6661 aaaatcaatc aattttcata gagctaggtg gaaagaaatg gtaaaaccct attttgaaaa
     6721 aaatgaaatt tttaaaatcc aacaggatgt tattttctaa aataaatatg gtagataacc
     6781 aatataaaaa gcatcaaaat gaacaaggag gaatctgatg cttggagcaa ttattggaga
     6841 tattgtcggt tctgtttacg aatggaacaa tatcaaaaca aaggactttc ctttatttcg
     6901 gaaggactgc ttttttacag atgacacggt tatgacctgt gctgttgcag aagcaattat
     6961 gaatggtgga caaaaagacg acttcattga cgcgatgaag aaatatggca gaatgtatcc
     7021 gaatgctgat tacggtgctc ggtttaatgc atggctaaac agcgataacc gtgagcctta
     7081 taatagcttt gggaatggat cagctatgcg tatttctcca tgtgcttgga tcatggactg
     7141 tggtttttat gcgaaaactg gtatatggcc atcatctaga ggacttacga gtctttctgc
     7201 agaggtaact cataatcatc cagaaggtgt caaaggcgct atggctacag ctgatgctat
     7261 ctttctatgt cgtttttact ttggtggtta ttgtagagag tacgagcaat caattaacga
     7321 caatcctaca gagtgtaaaa gacgaattaa ggattatata gaaaaagaat acgattacaa
     7381 tctatctcaa actttagatg aaatccgtcc taactatcgt tttaacgaaa catgtcagga
     7441 aactgtccct caagccatta tcgcctttct tgaaagtaga gacttcgaag atgcgataag
     7501 aaatgccatc tcacttggtg gcgacagtga tactctcgca gcaatcactt gtagcatagc
     7561 agaggcagct tacggtattc ctgattggat caaggataag gcctattctt acttggatga
     7621 acccttaaag gatgtagtta ggcgatggga aaatagaata aaagcgtatt aatctccagt
     7681 acgtcagatt tctcttttct gtattttcat tgtataatag gattacattt acttgcatac
     7741 gacgaaatgt aaacatttct gtttaaaatt aaggataaaa tcaatgaaaa aagcaatggt
     7801 aattatcaac cctacttctg gtggcgagaa ggctttggat tacaaagaaa agctggagaa
     7861 taaagcaaaa gaatactttg aatatgttga aaccaaaatt accgaaaaag cgctggatgc
     7921 aacacatttt gctgaagaag cttctcgtga gcagtatgat gcagtggttg tgtttggtgg
     7981 agatggaact gtcaatgaag tcatttcagg tattgatgag agagactaca ttcctaagtt
     8041 agggattatc ccaggcggta cgggtaacct cattacaaaa cttttggaaa tcaatcaaga
     8101 catcgatggc gcaattgacg aactggattt tgatttaacc aataagattg atatcggtaa
     8161 agcaaatgac aactattttg gttatatctt tagtatcggt tctctgcctg aggcgattca
     8221 caatgttgaa atagaggaca aaacaaaatt cggtatttta acctatgctg taaataccat
     8281 gaagtctgtc atgacagatc aggtctttaa cattaaggtt gagacagaaa atggaaatta
     8341 tgttggtgaa gctagccatg ttttggtcct tttgacaaat tacttcgctg ataagaaaat
     8401 ctttgaagaa aacaaggacg gctatgccaa cattttgatt ctgaaagatg cctctatatt
     8461 ctccaaatta tccgtcattc ctgatttatt aaaaggggat gttgtcgcaa atgataatat
     8521 cgagtatatc aaagcgcgta atattaaaat ctcttcagat agtgaattgg agtcagatgt
     8581 tgacggagat aaatcagata acctacctgt agaaatcaaa gtcctagctc agcgagtaga
     8641 agtattttca aaaccgaaag aggattagta tatagagaaa gcctttttta aggctttttg
     8701 tatactttaa aagatagttc ctttaacaac ggacattcct tgcaaatagt ttacaaaaat
     8761 agtatactgg atttattgag tttgaaaacg tttgcgtaaa atttgaatga atactttagg
     8821 agacaaattg atggaattga gtgctattta ccataggcct gagtcggagt atgcctatct
     8881 ttataaggat aagaaactcc atattcgaat tcgaactaag aaaggggaca ttgaaagcat
     8941 caacttgcac tatggggacc cttttatctt tatggaggag ttttatcagg atacaaaaga
     9001 aatggtcaag ataacttctg gtaccttatt tgaccattgg caggttgaag tgtcagttga
     9061 ctttgcacgt atccagtatc tctttgagct cagagataca gaaggtcaaa atattttgta
     9121 tggcgataaa gggtgtgtgg aaaattctct agaaaatctt catgcaattg ggaatggatt
     9181 taagttgcct tatcttcatg agattgatgc ctgcaaggtt cctgactggg tttcaaatac
     9241 ggtatggtat cagatatttc ctgaaagatt tgccaatggc aatgctctat taaacccaga
     9301 agggacttta gactgggatt catctgtcac acctaagagc gatgatttct ttggtggtga
     9361 tttacagggg attattgatc atatggatta cttgcaagac ttgggtatta ctggactata
     9421 tctttgtccc atctttgaat ctacaagcaa tcacaagtac aatacgacag attactttga
     9481 aattgaccgt cattttggag acaaggagac ctttcgggaa ctggtggatc aagcgcatca
     9541 tcgtggcatg aaagtcatgc tggatgcggt atttaatcat attggttcgc aatctcttca
     9601 atggaaaaat gtcgtcaaaa atggtgaaca gtctgcttat aaggattggt tccatattca
     9661 acaattccca gtgacaactg aaaagctagt taataagaga gacttaccct atcatgtttt
     9721 tggtttcgag gactatatgc ctaagctaaa tacagccaat ccagaggtca agaattatct
     9781 tttaaaggtt gcgacttatt ggattgaaga gtttaatatc gatgcttggc gtttggatgt
     9841 ggctaatgag attgaccatc agttctggaa ggattttcgt aaggcagttt tagctaaaaa
     9901 tcctgatctt ttcgagatta taaaaaatat aaagaatcta gtcataaatt tgaattgaaa
     9961 caacgtcttt attttttgct tcagcaatct aaatcgtttg atgatttttt agagaaagca
    10021 aagcagttgc atgttcagat tgattttagt caaaagcata gccgtttttt gatgacagat
    10081 agaacgatga taaaaccaat tcgagggcgc caactcagca aacgagatct atatgatgaa
    10141 gaatttttta gaacgcattt tgctaagcaa gagattgaaa gtcgtttaga atttttgtta
    10201 aaccgtgtca attctttaga agatttaata acaaaagcaa aagaattgaa tctaaccatt
    10261 gacttaaaac aaaaaatgta acttttatcc tggaagaaaa taatcaaaag ataagtttgg
    10321 gtcatcaaaa aataagtgat aagaaattat atgatgtcaa attttttcaa gattatttta
    10381 aaaataagga agtcgttgct tcagaaggat tagagaattt acaggaacag taccatgctt
    10441 tccaagaaga acgagataag gataaagtat ccactgaaga gattgagaaa gcctttaaga
    10501 catttaagga aaaatgagat accgttcgtg aatttgaagt ggaacttgca gagaaccaaa
    10561 tagagaagct agttgataag ggcgtctata tcaaggtgtc tttcggtgtc aaacagagcg
    10621 gtcttatttt cattcccaac tatcaattgg atattatgga agaagaaaat cataaaaaat
    10681 ataaagtcta cattcgagaa acgtcttctt actttgtcta taataaagaa aacatggata
    10741 ataattgttt catcaagggt aggaccttaa tcagacaatt gagcaacgat agccaaaaac
    10801 tcccctatag acgaccaact ttaaaaagtc ttcaagagaa gatttctgaa attaatctta
    10861 tgattgaact atctaacacg aacaaacaat accaagaaat caaagatgag catgtattag
    10921 aaattgcaga gattgatatg aagctagaag aaacccaaga aaaaatcgcc accttaaaca
    10981 agatggcgga agtccttatc aatttgaaga gtgaagatca tgagactaga aaattagcta
    11041 agtatgattt tgaccaaatg aacatgacgg aatcaataat gttagatcga ctcaatacag
    11101 atattttaaa attacaacag gaacttggta atgaaattaa taaatatgag gagatagcta
    11161 gaagattgga tttgtttgtg aaaattatta ataccaataa atttacagtt ctaaaatttc
    11221 acgagaatgc cttactagaa taatattatt tacgactaat tagttaaaag atagagaatc
    11281 atttcattta ttgtttacaa gtttatcaaa atggctagta tagtgtaatt ttaacttgtc
    11341 ttctcctaaa atatcgagaa cttgtatagc ttgtttcatt gtttcaatac cactacttaa
    11401 ttgacgttta aattcatagt aaccggttgc atagagaaaa actgtctgtt cataaaagtt
    11461 taattcattc tctaacaatc cctttatctc attaattaaa taagagcaat tagaaaattc
    11521 ttctttatct atgctgagaa tgaaacaatt tatagctagc tgagtgacaa tccgcttatt
    11581 tgttttattt
//
LOCUS       DAVKJJ010000057        11477 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000057 DAVKJJ010000000
VERSION     DAVKJJ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11477
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.057"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(147..1778)
                     /locus_tag="VW144_001728"
     CDS             complement(147..1778)
                     /locus_tag="VW144_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEU9601141.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGFGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2071..7011
                     /locus_tag="VW144_001729"
     CDS             2071..7011
                     /locus_tag="VW144_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEU9601142.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANPELVSGESEHSTNEADKQNEGEHARENKLEKAEGVAIASETAEAASAAKPEEK
                     ASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAEEANKTEKEVQPDVPKNTEKT
                     LKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSN
                     TNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLF
                     FNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLG
                     EKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPA
                     DNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGK
                     LRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADR
                     TPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAE
                     YDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAF
                     STTPDYKFDDADAWKELTLSDNWTNEEFALSSLAGKTIYAVKLFFEHEGAVKDYQFNL
                     GQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKL
                     LTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFDWGMTVKDTSLP
                     KPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKP
                     RTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLD
                     KPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQ
                     VGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGK
                     EISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIR
                     LTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQEP
                     KKDYLVGDNLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLRYQGH
                     EVNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLK
                     DHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEAQELLAAKPNHPSGS
                     ALAPLLEKNKALVEKVDLSPEELATAKQSLKDLVALLKEDKSAVFSDSKTGVEVHFSN
                     KEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEK
                     DKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNK
                     VLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRVIIQGRDGLLRHVF
                     EVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQL
                     PNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            7101..8297
                     /locus_tag="VW144_001730"
     CDS             7101..8297
                     /locus_tag="VW144_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU9601143.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8433..8960
                     /locus_tag="VW144_001731"
     CDS             8433..8960
                     /locus_tag="VW144_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9601144.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            9037..9393
                     /gene="glnR"
                     /locus_tag="VW144_001732"
     CDS             9037..9393
                     /gene="glnR"
                     /locus_tag="VW144_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEU9601145.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9430..10776
                     /gene="glnA"
                     /locus_tag="VW144_001733"
     CDS             9430..10776
                     /gene="glnA"
                     /locus_tag="VW144_001733"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEU9601146.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10872..10991)
                     /locus_tag="VW144_001734"
     CDS             complement(10872..10991)
                     /locus_tag="VW144_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEU9601147.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
BASE COUNT         3671 a         2106 c         2486 g         3214 t
ORIGIN      
        1 tctaacaatg ttttagaaat agaggtgtac tattctagtt tcaatatact atatatctgc
       61 agaatactct ttcagtttca ttcagcctag tcttgcttag gaaaaagtaa aaagccatcc
      121 aattatgatg gatagcttct tggttcttag atttgttctg caaaaacctt ttcagcaagg
      181 ttcatagcgt ggtctgatac acgagtgtag tgtgagatga tgtcgataaa gttgacccca
      241 gcttgtgttg aacattcgcc tttgttgagg cgtttgatgt gggtttttct gagaacacgt
      301 tctatcttat tgattgcttc atgacgttct acaagactgc gtgctttttc aatatcattg
      361 ttttccacac tatccagagc atctttgata aagtcactag tttggcggta aacttcctct
      421 aattctttca aggcggcatc agaaaattca acatttttcc gttgaagata gtcagtcaga
      481 ttgagtagag cctccgtgtg gtctccaatc cgttccaaat cacgggagga atcaaggata
      541 ttggtaagca cttcactttc tttttggctg agagcttcgc ttgaaagggc aatgagataa
      601 cgtgttaatt gctcatcgat ggtgttaatt gcttcttcgg ttttatgccc tttttcagca
      661 actttttcat ccaaatcaat gatgtactta taggaaaggt caaaggcttt agcagcgtag
      721 tttcctaagt gcaagagctc tttcttagca tttcctagag cgatagatgg ggcctgtttg
      781 atgaaatgtt catcaagata taagggttcg tatttgacaa cctcgtcctc tccaggaata
      841 atcttggtta caaagtaagc cagagctccg ataaatggaa attggacaat ggtgttggta
      901 atattaaagg ttccgtgagc aaaggcgatg gtcatttccg gtgctagatt tagcgtagct
      961 tcaaaccaat gaatcaggac agtaaaagga actagaaaaa taacgcagac gactgttccg
     1021 ataacgttga aggcaacatg agctcctgct acccgtttag ctgcaatatt agcccctaaa
     1081 gaggcaatga tggctgtaat ggttgtcccg atattgtcac caaatagaac tggcaaagct
     1141 ccctgtaggt caattagatt gccggcgtag aggttttgta aaatcccaat ggtagccgaa
     1201 gaagcttgaa ttagcaaggt caagccggta ccgacaaaga cacccaaaac aggattctta
     1261 cttagctcaa tcatatagtc cttaaagacc tgtaaatcct tgagtggagc cattgcgccg
     1321 ctcatgagat tgagggcaaa aaagatacca ccgaaaccaa agaggatgcg tccgatatta
     1381 ttgactgtcc gattttttgt aaaaaagaga cagacggcac cgataaagag cataggtagg
     1441 gcatagttac ctaatttaaa accgatgaga aaggatgtga cagttgtccc aatattagca
     1501 cccatgacaa tcccgatagc ctgacgtaag gttaagagac cggcactgac caggccgact
     1561 gtgataactg ttacaccaga actagactga attagagcag tcatcccaat accaaccaga
     1621 actccaaaga aaggattact agtatatttg tcaatgtaaa aacgaaggcg atctccagca
     1681 gcttgttgca aaccgtctcc catggtcttg atgctatata agaatagccc cagaccacct
     1741 aaaaagtgaa ataaaatttc ctgccaatta atggacattt ctttttcctc cgaaaaataa
     1801 tcgcggaata tctcctattc tattttaaag gataaaagta aatctaacaa gtgttaatgt
     1861 caagtttgta aaagaaaatt ataattgata agtgtattat ttttaagagt ttttaacctt
     1921 attttagatt tgtgcaaaca gttattgttt tttgtctaaa aatgttttag gataaaaatg
     1981 tgattatcat ggttctagag tttgataaaa aactacagaa tataaagcgc tttcttgaaa
     2041 acaacaaaat caatctttta ggaggagaaa atgaagaatc cattttttga aagacgttgt
     2101 cgttacagta ttcgtaagtt atcagtagga gcctgctcgc tgatgattgg tgctgtttta
     2161 tttgctggtc cagccttggc tgaagaaact gcagttcctg aaaatagcgg agctaatcca
     2221 gagcttgttt caggagagag tgagcattcg accaatgaag ctgataagca gaatgaaggg
     2281 gaacatgcta gagaaaacaa gctagaaaag gcagaaggag tagcgatagc atctgaaact
     2341 gcagaagcag ctagcgcagc taaaccagag gaaaaagcaa gtgaggtggt tgcagaaaca
     2401 ccatctgcag aagcaaaacc taagtctgac aaggaaacag aagcaaagcc cgaagcaact
     2461 aaccaagggg atgagtctaa accagcagaa gaagctaata agactgaaaa agaagtccag
     2521 ccagatgtcc ctaaaaatac agaaaaaaca ttaaaaccaa aggaaatcaa atttaattct
     2581 tgggaagaat tgttaaaatg ggaaccaggt gctcgtgaag atgatgctat taaccgcgga
     2641 tctgttgtcc tcgcttcacg tcggacaggt catttagtca atgaaaaagc tagcaaggaa
     2701 gcaaaagttc aagccttatc aaacaccaat tctaaagcaa aagaccatgc ttctgttggt
     2761 ggagaagagt tcaaggccta tgcttttgac tattggcaat atctagattc aatggtcttc
     2821 tgggaaggtc tcgtaccaac tcctgacgtt attgatgcag gtcaccgtaa cggggttcct
     2881 gtatacggta cactcttctt caactggtct aatagtattg cagatcaaga aagatttgct
     2941 gaagctttga agcaagacgc agatggtagc ttcccaattg cccgtaaatt ggtagacatg
     3001 gccaagtatt atggctatga tggctatttc atcaaccaag aaacaactgg agatttggtt
     3061 aaacctcttg gagaaaagat gcgccagttt atgctctata gcaaggaata tgctgctaag
     3121 gtaaaccatc caatcaagta ttcttggtac gatgccatga cctataacta tggacgttat
     3181 catcaagatg gtttgggaga atacaactac caattcatgc aaccagaagg agataaggtt
     3241 ccggcagata acttctttgc taactttaac tgggataagg ctaaaaatga ttacactatt
     3301 gcaactgcca actggattgg tcgtaatcct tatgatgtat ttgcaggttt ggaattgcaa
     3361 cagggtggtt cctacaagac aaaggttaag tggaatgaca ttttagacga aaatgggaaa
     3421 ttgcgccttt ctcttggttt atttgcccca gataccatta caagtttagg aaaaactggt
     3481 gaagattatc ataaaaatga agatatcttc tttacaggtt atcaaggaga ccctactggc
     3541 caaaaaccag gtgacaaaga ttggtatggt attgctaacc tagttgcgga ccgtacgcca
     3601 gcggtaggta atacttttac tacttctttt aatacaggtc atggtaaaaa atggttcgta
     3661 gatggtaagg tttctaagga ttctgagtgg aattatcgtt cagtatcagg tgttcttcca
     3721 acatggcgct ggtggcagac ttcaacaggg gaaaaacttc gtgcagaata tgattttaca
     3781 gatgcctata atggcggaaa ttcccttaaa ttctctggtg atgtagccgg taagacagat
     3841 caggatgtga gactttattc tactaagtta gaagtaactg agaagaccaa acttcgtgtt
     3901 gcccacaagg gaggaaaagg ttctaaagtt tatatggcat tctctacaac tccagactac
     3961 aaattcgatg atgcagatgc atggaaagag ctaacccttt ctgacaactg gacaaatgaa
     4021 gaatttgctc tcagctcact agcaggtaaa accatctatg cagtcaaact attttttgag
     4081 catgaaggtg ctgtaaaaga ttatcagttc aacctcggac aattaactat ctcggacaat
     4141 caccaagagc cacaatcgcc gacaagcttt tctgtagtga aacaatctct taaaaatgcc
     4201 caagaagcgg aagcagttgt gcaatttaaa ggcaacaagg atgcagattt ctatgaagtt
     4261 tatgaaaaag atggagacag ctggaaatta ctaactggct catcttctac aactatttat
     4321 ctaccaaaag ttagccgctc agcaagtgct cagggtacaa ctcaagaact gaaggttgta
     4381 gcagtcggta aaaatggagt tcgttcagaa gctgcaacca caacctttga ttggggtatg
     4441 actgtaaaag ataccagcct accaaaacca ctagctgaaa atatcgttcc aggtgcaaca
     4501 gttattgata gtactttccc taagactgaa ggtggagaag gtattgaagg tatgttgaac
     4561 ggtaccatta ctagcttgtc agataaatgg tcttcagctc agttgagtgg tagtgtggat
     4621 attcgtttga ccaagccacg tactgttgtt agatgggtca tggatcatgc aggagctggt
     4681 ggtgagtctg ttaacgatgg cttgatgaac actaaagact ttgaccttta ttataaagat
     4741 gcagatggtg agtggaagct agctaaggaa gtccgtggta acaaagcaca cgtgacagat
     4801 atcactcttg ataaaccaat cactgctcaa gactggcgct tgaatgttgt cacttctgac
     4861 aatggaactc catggaaggc tattcgtatc tataactgga aaatgtatga aaagcttgat
     4921 actgagagtg tcaatattcc gatggccaag gctgcagccc gttctctagg caataacaag
     4981 gtacaagttg gctttgcaga tgtaccggct ggagcaacta ttaccgttta tgataatcca
     5041 aattctcaaa ctccgctcgc aaccttgaag agcgaagttg gaggagacct agcaagtgca
     5101 ccattggatt tgacaaatca atctggtctt ctttattatc gtacccagtt gccaggcaag
     5161 gaaattagta atgtcctagc agtttccgtt ccaaaagatg acagaagaat caagtcagtc
     5221 agcctagaaa caggacctaa gaaaacaagc tacgccgaag gggaggattt ggaccttaga
     5281 ggtggtgttc ttcgagttca gtatgaagga ggaactgagg acgaactcat tcgcctaact
     5341 cacgcaggtg tatcagtatc aggttttgat acgcatcata agggagaaca gaatcttact
     5401 ctccaatatt tgggacaacc agtaaatgct aatttgtcag tgactgtcac tggccaagat
     5461 gaagcaagtc cgaaaactat tttgggaatt gaagtaagtc aggaaccgaa aaaagattac
     5521 ctagttggtg ataacttaga cttgtctgaa ggacgctttg cagtggctta tagcaatgac
     5581 accatggaag aacattcctt tactgatgag ggagttgaaa tttctggtta cgatgctcaa
     5641 aagactggtc gtcaaacctt gacgcttcgt taccaaggtc atgaagtcaa ctttgatgtt
     5701 ttggtatctc caaaagcagc attgaacgat gagtacctca aacaaaaatt agcagaagtt
     5761 gaagctgcta agaacaaggt ggtctataac tttgcttcac cagaagtaaa agaagccttc
     5821 ttgaaagcaa ttgaagcggc cgaacaagtg ttgaaagacc atgaaactag cacccaagat
     5881 caagtcaatg accgacttaa taaattgaca gaagctcata aagctctgaa tggtcaagag
     5941 aaatttacgg aagaaaagac agagcttgat cgcttaacag gtgaggctca agaactcttg
     6001 gctgccaaac caaaccatcc ttcaggttct gccctagctc cgcttcttga gaaaaacaag
     6061 gccttggttg aaaaagtaga tttgagtcca gaagagcttg caacagcgaa acagagtcta
     6121 aaagatctgg ttgctttatt gaaagaagac aagtcagcag tcttttctga tagtaaaaca
     6181 ggtgttgaag tacacttctc aaataaagag aagactgtca tcaagggttt gaaagtagag
     6241 cgtgttcaag caagtgctga agagaagaaa tactttgctg gagaagatgc tcatgtcttt
     6301 gaaatagaag gtttggatga aaaaggtcaa gatgttgatc tctcttatgc ttctattgtg
     6361 aaaatcccaa ttgaaaaaga taagaaagtt aagaaagtat ttttcttacc tgaaggcaaa
     6421 gaggcagtag aattggcttt tgaacaaacg gatagtcatg ttatctttac agcacctcac
     6481 tttactcatt atgcctttgt ttatgaatct gctgaaaaac cacaacctgc taaaccagca
     6541 ccacaaaaca aagtccttcc aaaacctact tatcaaccgg cttctgatca acaaaaggct
     6601 cctaaattgg aagttcaaga ggaaaaggtt gcctttcatc gtcaagagca tgaaaatgct
     6661 gagatgctag ttggggaaca acgagtcatt atacagggac gagatggact gttaagacat
     6721 gtctttgaag ttgatgaaaa cggtcagcgt cgtcttcgtt caacagaagt catccaagaa
     6781 gcgattccag aaattgttga aattggaaca aaagtaaaaa cagtaccagc agtagtagct
     6841 acacaggaaa aaccagctca aaatacagca gttaaatcag aagaagcaag caaacaattg
     6901 ccaaatacag gaacagctga tgctaatgaa gccctaatag caggcttagc cagcctcggt
     6961 cttgctagtt tagccttgac cttgagacgg aaaagagaag ataaagatta aatatcgaaa
     7021 aatcttgtga aatctttcct tatatttcca aagtgtgata taatagtttc gaataaaata
     7081 aataaaggag tttttgtaac atggcaaaac ttactgttaa agacgttgac ttgaaaggta
     7141 aaaaagtcct cgttcgtgtt gacttcaacg taccattgaa agatggcgta atcactaacg
     7201 ataaccgtat cacagcagct cttccaacta ttaagtacat catcgaacaa ggtggacgtg
     7261 caattctttt ctctcacctt ggacgtgtga aagaagaagc tgataaagct ggtaaatcac
     7321 ttgctcctgt agcagcagac ttggcagcaa aacttggtca agatgttgtt ttcccaggtg
     7381 tcactcgtgg tgctgaattg gaagcggcaa tcaacgctct tgaagatgga caagttctct
     7441 tggttgaaaa cactcgttac gaagatgttg acggcaagaa agaatctaaa aacgatcctg
     7501 aacttggtaa atactgggca tcacttggag atggtatctt cgtaaacgat gcattcggta
     7561 cagctcaccg tgcacacgca tctaacgttg gtatctcagc aaacgttgaa aaagcagttg
     7621 ctggtttcct tcttgaaaac gaaattgcct acatccaaga agcagttgaa actccagaac
     7681 gtccattcgt ggctatcctt ggtggttcaa aagtttcaga caagatcggt gttatcgaaa
     7741 acttgcttga aaaagctgat aaagtcctta tcggtggtgg gatgacttac acattctaca
     7801 aagcacaagg tatcgaaatc ggtaactcac ttgtagaaga agacaaattg gatgttgcga
     7861 aagctcttct tgaaaaagca aatggtaaat tgatcttgcc agttgactca aaagaagcta
     7921 acgcatttgc tggttacact gaagtgcgtg acactgaagg tgaagcagtt tctgaaggct
     7981 tccttggtct tgacatcggt ccaaaatcta tcgccaaatt tgacgaagct ttgactggtg
     8041 ccaaaacagt tgtatggaac ggacctatgg gtgtatttga aaacccagat ttccaagctg
     8101 gtacaatcgg tgtgatggac gctatcgtga aacaaccagg agttaaatca atcatcggtg
     8161 gtggtgactc agctgccgca gcgattaacc ttggccgtgc agacaagttc tcatggatta
     8221 gtacgggtgg tggagcatca atggaacttc ttgaaggtaa ggttcttcca ggacttgcag
     8281 ccttgacaga aaaataagat tttataaata aatcaaagaa gagagggatg aaagttcctc
     8341 ttttcttttg cttaaaataa aaacgcttcc tctcaactat tactcataaa aatcaccgat
     8401 ttatgataaa atggaaatag aaagttgaga ttatgagtta ttttaaaaaa tataaattcg
     8461 ataaatccca gttcaaactt ggtatgagaa cctttaaaac aggtattgct gtttttctag
     8521 ttctcttgat ttttggcttt tttggctgga aaggtcttca aattggtgct ttgacagccg
     8581 tttttagcct gagggagagt tttgatgaga gtgttcattt tgggacttcg cgtattctag
     8641 gaaatagtat cggtggactc tatgccttgg tcttcttctt attaaatacc tttttccatg
     8701 aagccttttg ggtgaccttg gtagttgttc caatctgcac catgttaacc attatgacaa
     8761 atgtagccat gaataacaaa gcaggggtta ttggtggtgt agcagctatg ttaatcatta
     8821 ccctatcaat tccaagtggt gagacaattt cgtacgtgtt tgtgcgtgta tcagaaacgt
     8881 ttatgggagt ttttgtcgca attatcgtaa attacgatat tgatcgtatt cgtctctttt
     8941 tagagaaaaa agaaaaataa tgttacattt tataacatta tcaattgacg tttgtctttt
     9001 tttagactat aatagacaga aagaaggaaa ttgtaaatga aggaaaaaga atttcgccga
     9061 aatatggctg tttttcctat cggcagtgtt atgaagttga ccgatttatc ggcgcgtcag
     9121 attcgttatt atgaagatca agagttgatc aagcccgatc gaaacgaagg aaatcgtcgc
     9181 atgtattcct tgaatgacat ggatcgtctg cttgaaatca aagattatat ctctgaaggt
     9241 tataatatcg ctgccattaa gaaaaaatat gctgaacgtg aagcgaaatc caagaaagcg
     9301 gttagtcaga ctgaagtacg tcgtgcactt cacaatgaac tcctccaaca ggggcgtttt
     9361 gcttcagtac agtcaccttt tggtcgcggt taggcaaccg caagtagtca tagattaagg
     9421 agaaaactta tgccaatcac agctgcagat attcgtcgtg aagtcaagga aaaaaatgtt
     9481 acctttattc gtcttatgtt ctcagatatt ttgggaacca tgaaaaacgt cgaaattcct
     9541 gctacagatg aacagttaga taaggtcttg tcgaacaagg ttatgtttga tggatcttct
     9601 attgaaggtt ttgtacgtat caatgagtcg gatatgtact tgtacccgga cttggataca
     9661 tggacagtct tcccttgggg agatgaaaat ggaagtgttg caggtctgat ctgtgatgtt
     9721 tatacaacag aaggtgaacc atttgcgggt gatcctcgtg gtaatttgaa acgagctctt
     9781 cgtcacatgg aagaagttgg attcaaatcc ttcaaccttg gtccagagcc agaattcttc
     9841 ctatttaagt tggatgaaaa tggggaccca acacttgaag tgaatgacaa gggtggctac
     9901 tttgatttgg cacctactga ccttgcggac aacacacgtc gtgagattgt gaatgtcttg
     9961 accaaaatgg gatttgaagt agaagcgagt caccacgagg ttgcggttgg acagcatgag
    10021 attgacttta agtacgatga agttctccgt gcttgtgata agattcaaat ctttaaactt
    10081 gttgttaaaa ccattgctcg caaacacgga ctttacgcaa catttatggc gaagccaaaa
    10141 tttggtattg ctggatcagg tatgcactgt aatatgtcct tgtttgatgc agaaggaaat
    10201 aacgccttct ttgatccaaa tgatccaaaa ggaatgcagt tgtcagaaac agcttaccat
    10261 ttcctaggcg gtttgatcaa gcatgcttac aactatactg ccatcatgaa cccaacagtt
    10321 aactcataca aacgtttggt tccaggttat gaagcgcctg tttacattgc ttgggctggt
    10381 cgtaaccgtt cgccacttgt gcgcgtacct gcttcacgtg gtatgggaac tcgtcttgag
    10441 ttgcgttcag tggatccaat ggcgaaccct tacattgcta tggctgttct tttggaagtt
    10501 ggtttgtatg gtattgaaaa taaaatcgaa gcaccagctc ctatcgaaga aaatatctac
    10561 atcatgacag cagaagagcg caaggaagct ggtattacag accttccatc aactcttcac
    10621 aacgctttga aagctttgac agaagatgaa gtggttaaag ctgctctcgg agatcacatc
    10681 tacactagct tccttgaagc caaacgaatc gaatgggcaa gttatgcaac cttcgtttca
    10741 caatgggaaa ttgataatta tttagacctt tactaatact aatatagaag aaagattgcc
    10801 tgtgggtaaa cagtcacaca gcagtctaag ccatcaaata cctataaccc ttaaaatagc
    10861 gatttcggtc gctatttatt tttcgaaaaa tcctcttgac tcttttgcga attagattta
    10921 tcatcagacg tgtagaagaa aattaaggag tcactatgga aaaattcaat ttcaaaaaca
    10981 atatcgggca agaaaacaag ttacttcaaa tcgaaatcta caagtttact aacttttgca
    11041 agcttcaaaa ttacacatct gtaaatattt tttcgaaaga tatatttgaa accatagtaa
    11101 attgagacta gaatagtaca ctacatctgc taaaaatttc tagaaactaa tttaactttc
    11161 ctaatcgatt tgtttacatc ttatttcaat ctactataat tatctcaata aatgatgatg
    11221 gcgactgttt tgtttgtgct tgtcgatttt tgaatcctga tcgagcggaa gaatatttct
    11281 gccgaggttg ccaaggggaa aatactgaag atattgaatt ttatgatgaa caattacagg
    11341 cagaaaaggt ggaggttttg aatattcgat tggaagtaaa aaattgaatt atctacaatc
    11401 tcatcaacaa taaacctatt tcaaccccat tatctatagg cgtattttta cgccttttta
    11461 tatacttgaa gcacata
//
LOCUS       DAVKJJ010000058        11192 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000058 DAVKJJ010000000
VERSION     DAVKJJ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11192
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.058"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(144..2033)
                     /locus_tag="VW144_001735"
     CDS             complement(144..2033)
                     /locus_tag="VW144_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEU9601148.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFEKDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGGEKALQVGMG
                     ALTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDQAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSLFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2171..3736
                     /locus_tag="VW144_001736"
     CDS             2171..3736
                     /locus_tag="VW144_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9601149.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            3736..4476
                     /locus_tag="VW144_001737"
     CDS             3736..4476
                     /locus_tag="VW144_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9601150.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(4585..4800)
                     /locus_tag="VW144_001738"
     CDS             complement(4585..4800)
                     /locus_tag="VW144_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601151.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(5671..6921)
                     /gene="ilvA"
                     /locus_tag="VW144_001739"
     CDS             complement(5671..6921)
                     /gene="ilvA"
                     /locus_tag="VW144_001739"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEU9601152.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(6971..7327)
                     /locus_tag="VW144_001740"
     CDS             complement(6971..7327)
                     /locus_tag="VW144_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9601153.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEIFGDE"
     gene            complement(7565..7801)
                     /locus_tag="VW144_001741"
     CDS             complement(7565..7801)
                     /locus_tag="VW144_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601154.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKY"
     gene            complement(7908..8930)
                     /gene="ilvC"
                     /locus_tag="VW144_001742"
     CDS             complement(7908..8930)
                     /gene="ilvC"
                     /locus_tag="VW144_001742"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9601155.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(8996..9472)
                     /gene="ilvN"
                     /locus_tag="VW144_001743"
     CDS             complement(8996..9472)
                     /gene="ilvN"
                     /locus_tag="VW144_001743"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU9601156.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(9465..11165)
                     /locus_tag="VW144_001744"
     CDS             complement(9465..11165)
                     /locus_tag="VW144_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEU9601157.1"
                     /translation="MEKISLESPKTGSDLVLETLRNLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
BASE COUNT         3380 a         2553 c         1929 g         3330 t
ORIGIN      
        1 gctttttgcc gtgctctaaa atcatcgcca aatcatcaaa ttgaaataag aaatagttta
       61 gataaaacta ttgacttttc ttatagtttg tcttttttct atcttccatt atatcatact
      121 ttttcagtct atggtagctc tctttaaaag gcaccgatac tgccgccacc tccgcctcca
      181 gagaagccac caccagaact tccacttcca gaagatacag agtaggtgct tgctgtattt
      241 gcgacactag cataatggct catttgcgcg cttgaatgat aaaacagact gtgccagcca
      301 taagctacat agagattgat atctggattt tccacttgaa tctgatgaac cttcatcaaa
      361 tgactaacct tgtccgcata gccaaataag gtcgcataga ccaagaggcg attccagacc
      421 acaatacttt ccagttcagc ctgatccaat cgtgcaatct cacgcaacat attttcaaaa
      481 ctggtccaga gatagtagac ctcagctccc gcttcattta gaacaccatc acgattatct
      541 agtcgaagct tccaataata gaaaacagac aaaactaacc ctagaaaacc aagtattggc
      601 aaagggaggt aaagatagcc atgaacgtct aaactgtaca agaacaaacc aaatccgata
      661 aataggggca ggatagtcaa ggcacccata cccacttgca aggccttttc cccaccagtt
      721 aaaggacgat aataatctgg gagcccccaa aaggaaactc gttttctcac tccttcttgc
      781 atctggttca atacctcttc aaaagaagat ttgagttgaa gccctcttgc ttgaatccgt
      841 ttttcatcag aaactttggc tctacgataa agactatcag ataccttgta atccgcaaac
      901 aaattggaaa gagtttcttc ttttttacct gaaaaagcta gatttaggca gtctttctca
      961 aagcttgaca aaccatcttc ttttactagc ctcaaaccaa ctgcatctcc ttctgaaatg
     1021 atagagacat tcccacggtc tatcacatct agcaaggtag cttgaataag ttgatcaaag
     1081 gtgaattttc cagctccctt gaccaaggga ctcacttcct ccaaggaggt cgagtagact
     1141 gcttctgata aaaccatagg ctctaattcc attggtggtt catagagacg atgatttttg
     1201 gcatatttga ctgaaggagt ggtctttctt ctataaataa aatagaagca gacactcaat
     1261 aacaaggaga tagaaaggat cgaagggagg acccaagtaa caagttgttt actctgatct
     1321 ttttctctaa caatcgagtc ttctatctta ttaaactctt ctaaacgatt ccctttcaat
     1381 ccctgatccc tagcgctagc aaaatcggtc cgaggccaat aggcatgcaa ctcaactcca
     1441 cgcttagccg gaagattgtc taaacggata gtataatcaa ggttactctt ttcaatcgtt
     1501 ccctctctaa aaagtttccc tgtatggaaa aagagttttt cagctccctt gtctcccctt
     1561 acatgaaatt caaacttttc aatagactct gaactatctg tcagaggttg ccaatttaat
     1621 tcagcgatat catcataaag gaaaagtaaa ttttttaagt tccagacgag gtcaacttca
     1681 actatgtcgc cctcctgacc tggattatag actctcacag tataaccatc cgcttcttct
     1741 gttacttcgc tagtcacatc tgctagttct gcaccgtttt tcgcggcctg aacctttgga
     1801 tgagggtcaa tgtcaaaccc gctaggcatc ttaccagcac gtccaagtcc cacgatttgt
     1861 cccttaaagt cctcctcaaa ctggtaaact atcttctgtc taaactctgc cgtattatct
     1921 gcatgaatat acaaatcccc ttgataagag tttatcttga aatcaatggc aaaaacagag
     1981 agtggaagaa ggcaaaacaa gcctaacacc agtaagaaaa aagttttttt catcaactaa
     2041 gccccctttt tatctttctt atcattatag cattttttcg taagtaaagg cttacttatg
     2101 ttgaaaaatt acgcattttt cgataaaata ggagacagtt attttttaat agatcagaat
     2161 aggagaaatc atgagaaaaa tatacttatc tattttcaca agtctcttgc tgatgctagg
     2221 acttgtcaat gttgctcaag ccgatgaata tttacgcatc gggatggaag cagcatatgc
     2281 tccctttaac tggacccagg atgatgatag caacggagct gtcaaaatcg atgggaccaa
     2341 tcagtatgcc aacggatacg atgttcaaat cgccaagaaa atcgctaagg acttaggtaa
     2401 agaacctttg gttgttaaaa ccaagtggga aggtctagtc cctgccctta cttctggtaa
     2461 gattgacatg attatcgcag gtatgagtcc aactgcagaa cgcaaacaag aaattgcctt
     2521 ttcgagcagt tactacacta gcgaaccagt tttgcttgtc aaaaaagatt ctgcctacgc
     2581 aagtgctaaa tctttggatg actttaatgg tgcgaaaatc acttctcaac aaggtgtcta
     2641 cctctacgac ttgattgcac aaatcccagg tgctaaaaaa gaaacagcca tgggagactt
     2701 cgctcaaatg cgccaagctc ttgaggctgg tgtcattgat gcttatgttt ctgaacgtcc
     2761 agaagcactg actgctgaag ctgcgaactc taagttcaag atgattcaag tagaacctgg
     2821 tttcaaaact ggggaagaag atacagctat cgctatcggg cttcgtaaaa atgacaatcg
     2881 tattagccaa atcaatgcca gcattgaaac catttcaaaa gatgaccaag ttgccttgat
     2941 ggatcgtatg atcaaggaac aacctgccga agctacaaca actgaagaga ctagcagtag
     3001 tttctttagc caagtcgcta aaattctttc tgaaaactgg caacaactct tgcgtggtgc
     3061 tggtatcact cttttaatct ctatcgtcgg aaccatcata ggtctcatta ttggacttgc
     3121 cattggtgtc ttccgtactg ctcctctctc tgaaaacaaa gccatttatg gccttcaaaa
     3181 actagtcggc tgggttctca atgtctacat tgaaattttc cgtggtacgc caatgattgt
     3241 tcaatcgatg gttatctact atggaactgc ccaagctttc gggatcaacc ttgaccgtac
     3301 actggctgct atcttcatcg tttcaatcaa taccggtgcc tacatgactg aaatcgtccg
     3361 tggtggtatc ctagcagttg acaagggaca atttgaagct gcgactgctc ttggtatgac
     3421 ccataaccag accatgcgta agattgtcct acctcaggta gtccgtaaca tcctacctgc
     3481 aactggtaat gaatttgtca tcaatatcaa agatacatct gtattgaacg ttatctctgt
     3541 tgtcgaactt tatttctcag gaaataccgt ggcaacacaa acctatcaat acttccagac
     3601 atttacaatc atcgccgtga tttactttgt cctcaccttc accgtaacac gtatcctacg
     3661 atttatcgag cgccgaatgg acatggatac ctatactaca ggtgctaacc aaatgcaaac
     3721 ggaggatttg aaataatgac acaagcaatc cttgaaatta aacacctcaa aaaatcctat
     3781 ggacaaaacg aagtgctaaa agacatttca ctcactgtcc acaagggaga ggtcatctct
     3841 atcatcggaa gctctggaag cggaaaatcg accttcctac gctccattaa cctacttgaa
     3901 acaccaactg atggacaaat cctttatcat ggacaaaacg tcctcgaaaa aggctatgac
     3961 ctcacgcaat accgtgaaaa gttggggatg gttttccaat cctttaacct ctttgaaaat
     4021 ctcaatgttc ttgaaaacac aatcgtcgct cagacaactg tcctaaaacg cgaacgcaca
     4081 gaagctgaaa agattgccaa agaaaacctg gaaaaggtcg gcatgggaga acgctactgg
     4141 caagccaaac caaaacaact ctcaggtggt caaaaacaac gtgtggccat cgctcgtgcc
     4201 ctctccatga atccggacgc tattctcttt gatgaaccaa catcagctct cgatccagaa
     4261 atggttggag aagtcctcaa aatcatgcag gacctggctc aggaaggctt gaccatgatt
     4321 gtcgtaaccc atgaaatgga atttgcccgt gatgtctctc accgtgttat ctttatggat
     4381 aagggcgtga tcgctgaaga aggtaaacca gaagacctct tcaccaatcc taaagaagac
     4441 cgaacaaaag agttccttca acgctatctc aaataaaaag aaaaggctgc atcaaccgtg
     4501 cagtcttttt gctgtcctca aaatgaaaag gcagattctc tatctctact acttgaaggt
     4561 caagaatccg ccgttatcca aagattaatc ttcaaatttt tcataatttt tttcatagaa
     4621 atcaattaag cctagggctg tttcaaatga aatatttttt acttttgcac gaccctgcgc
     4681 cagagcaatg atagacattt cacgcgcatt cgtttcttta gagatacggt agcctgtgat
     4741 tttcttatca cgaacccagc caacaactga ttctactttt tcaaagtttg acttagccat
     4801 gtttttctcc tattttcttc tttataatat ttaattgtat acgtttttac tacttttgtc
     4861 aataaaaaaa acatatattc aacgaaaata attagttcag aattttctta cttccttttt
     4921 aaatccgata atatataggt tttgctagcc tctacaccat aaatatttgc taataaaact
     4981 actgtattat taccctctta aggtgcgaaa ttattcaagt tcttaaaaaa tgcttctcta
     5041 tttccgtaag atagcttatt tcttcgattt aagaactgaa gatgatagtt ctaaccttta
     5101 gaaatccaag ccttcactag ctcaaaaact cctatctttc ttcctcaata aatcatctga
     5161 gagataggta ttccttatat ccttcaaatt catcactcct ttggatattg aggtgctagc
     5221 ctagaagcag acacccgatt atctaaacca tccttaatag agatgacaag acagataagc
     5281 tcagttaatt acttatatat ctttttaata tggtatattt gtcaacaaaa agaaatcaaa
     5341 tcatacctta tactcaatga aaatcaaaga gcaaactagg aagctagccg caagttgctc
     5401 aaaacactgt tttgaggttg cagatagagc taacgtggtt tgaagagatt ttcgaagagt
     5461 attaaagaca gtgtgactat gcttgtcaat agataggaaa tcaaaatgaa ggatggaaat
     5521 ctgagccaaa catcataagt gctataatga acagaagtaa gaattgcacg ataagttttc
     5581 taaaaccata ggaatcgtcc cctttcttca tcttcattat agcatatata catcagttgt
     5641 acaaataata tgatattttt ttattagtcc tcagacaagc atattataaa gcgtttcatt
     5701 accatttaag ttaatataag ctggatcaaa accttccatt ctacgaatca aacctgcata
     5761 atcatgctta tctgctaaag cgatcccaat caatactggg cctgttccct tgctagctcg
     5821 tttgatatac tcaaaacgtg tgatatcatc atttggcccc aggatatcat ttacaaactc
     5881 acgcaaagct cctggacgtt gtgggaaatt gaccacaaaa taatgtttga taccatcata
     5941 aatcaaggca cgctcttcca tttctggcat acggttgata tcattatttc ctccagaaat
     6001 gatacaacaa atggttttcc ccttaatata ttcagctaaa acctctaaag aggcgatact
     6061 agctgctcca gcaggttctg cgactatccc ttgcttagag taaaggtcaa tcaaggtttc
     6121 agaaatcaat ccctcatcga cacctaccaa agtttgaata tgttgacgag ttgcttcata
     6181 ggtcaactga cctacctttt gcacagcaat cccatccgca aatttatcaa tttccttgag
     6241 tttgactgga cctccagcct caaaggcagc tttcatggaa cgcgctccat tcgcctctac
     6301 tccgataacc tcaatctctg gacttgtttc cttgatatag gtagaaaccc cggcaatgag
     6361 accgccacca ccaacaggaa ccaagacagc atcaaaatca atcgattctt ttcgagcttc
     6421 ttctaaaatc tcataagcaa ctgttccttg acctgcttga acatgagcat catcaaaagg
     6481 atcaataaag gtacgatttt cagagactgt aaattcttga gctgctttgg ctgaggcatc
     6541 aaaggtatct ccaactagtt taatagttac aaaatcccca ccaaaaaagc gaacctgacc
     6601 aatcttttgt tgtggcgtag taatgggcat aaagatagta gcaggaattt tcatttcatt
     6661 acaagtatag gctactccct gcgcatgatt tcccgcagaa gcgcaaacta ccccacgttc
     6721 acgttcttcc ttgctgagct gggaaatggc ataataggca ccacgaattt taaaggagcg
     6781 aacacgctgg gcattttctt ttttcaaata aatcttagca ccatacttct ccgataaata
     6841 atgatcgtaa tccagtggag tattcacaac cacaccgttc aagaccttgt gagccttgat
     6901 tatatctttt gaacttaaca tataaaacct ctttttctat aaaaaacagt cactaatgac
     6961 tgttttttag ttattcatct ccgaaaattt cagtaacact ttcatggtat ttatgagcca
     7021 tacgaatcga aaactcttta ggaagttcat aacgatttgt ggaaatccaa tcatataacc
     7081 attctaccaa actttcttca ctatcattac gatggaagat aaaatagttt aaaatttctt
     7141 tatattctac atcattcaaa tccaacgtgt ctacaaattg actacgagga acaaaattag
     7201 aatctaattt tcttagttga tctgtcaatt ctcccaattg taaatccaac tgaaaaaata
     7261 gtttttgcag ttgcctatca gtttcatcac atcctgtctt gatagaaaac aaattggtaa
     7321 tattcatatg atttcaaccc ctaaataagt acatctaata tcccacgaat agctgaagct
     7381 aaagttttcg cagtccagtc atttaaaccc gattctttca atccagatgc aataatttgc
     7441 tctatttgat ataaaccgcc atctatcgtc tcatttattc tgcgaagagg accttcaata
     7501 taggataaag cttctccgaa tctaacagct gatttaggtc ctaatgattt accaactata
     7561 tttgttaata ttttttattt ctcaaaatcc atttcgctat ataacgagca gatttagtcg
     7621 caccagctct ctcaaacgga gaatgaattt caagaccttc ttctctagct atttgtgcca
     7681 atttgaagaa ttgttcaaag gtcatatgca acctaccatc ttcaaaatta gcatcatcat
     7741 aaatgttaat tacagcattt tcatcatacg accaatctgc tttattttgg tctgctagca
     7801 ctattaccga cggtgtaatt acattataca actagaacca ataataacga tgcaattttt
     7861 ctcataatct ttttcataaa atcatctcca gcgctatata tttctaatta gttatagatt
     7921 ttgaatgcat catcgtcgtt tttaccaacg aatggcattg ctttacgcaa ttctgcacca
     7981 actttttcaa tttcaaggtt agctgcttgt tcacggtaag cagtcaattt tggacgtcca
     8041 gctttatagt catttacaaa gtcatttgca aatttaccat tttggatgtc tgccaagaca
     8101 gccttcatat tttctttaac ttgttcagtg attacacgtg gacctgatac atagtcaccg
     8161 tattcagcag tgtttgaaat agattgacgc attttcttga atccaccttc gtagatcaag
     8221 tcaacgatca atttcatttc gtgaagaact tcaaagtaag ccaattctgg agcgtaacct
     8281 gcttctgtca agacttcgaa acctgcttcg ataagggcag tcaaaccacc acaaagtaca
     8341 gcttgttcac caaacaaatc ttcttcagtt tcttctttgt aagttgtttc aagaagacct
     8401 acacgagccg ctccaacacc tttacaccag tccatagcaa tgtttttagc atttcctgtt
     8461 gcatcttggt atactgcata aagagctgga acaccaaatc cttcttcgta agtacgacgt
     8521 accaagtgtc ctggtccttt aggagcacac atgaagacat ctacatccgc aggaactttg
     8581 ataaattcaa agtggatgtt gaaaccatgg gcaaatccaa ctgcgtttcc agcttccaag
     8641 tttggagcga tttctgcttc gtacaattct tgttgaattt cgtctggcgc caagatcatg
     8701 ataacatcag ccaacttagt agcttctgct actgtgtaag tatcaaatcc atcttctttt
     8761 gctttatcaa aagatttacc tggacgtaca ccgataataa cgtcacgacc tgaatcacgc
     8821 aagttttgag catgcgcatg cccttgtgaa ccataaccga taacggcgat ttttttaccg
     8881 tcaagtgctg ctactttaac atctttttca tattccattt gaactgtcat agcttttctc
     8941 tcttttctat tatttattgc cttttaagct ggtttaacaa atttaagttg gatttttaat
     9001 cgcgggtaaa tccagttgca cccgttcgag caatattgcg aataccgtat gggcgaatga
     9061 ctcgcaatag ggcttcgctc ttttctgcat ttcccgtcat ctgaatggta atcgagcttg
     9121 gcgctacgtc taccactgtt gcacggaaag gttgaataat cgctaaaatc tcagctctct
     9181 tctcagctgg cgctgacatc ttaaccaaaa tcacctcgcg ctccaaatga ggcttgtctg
     9241 taatatctcg aatgcgaatc acatcaatct gacgattgag ctgtttgatg atttgctcca
     9301 cttcatcatg agaagcaaca tcaataataa tagtgatacg cgatacattc ggatcttctg
     9361 ttgctccaac agagatgctt tcaatattaa cctgacgacg agataggaca cctgtaaagc
     9421 gattgaggac tcctgaacga ttttgtagtt ttgctgttaa cattctacgc atggaactgc
     9481 acccccaaca tctcatgatt actcttacca gccggtacca ttggtaacac ctgttcctta
     9541 cgagaaatat ctacctcaat tagcatagga acatcctcag tgatgacttc aaggtcttga
     9601 gccaaggtct caggattgtc aaacttatag tttttaatac cataagcctg cgccatcaat
     9661 tggaaatcag gaagggtatc aaagaccgac tctgatgttc tgccttcata gaaggattcc
     9721 tgccactggc gaaccattcc aagtgaatga ttgttcagca taaccacctt gattggcacc
     9781 ttgtaaatat tcaaaatagc caactcctgg ttggtcattt ggaaaccacc atccccaaca
     9841 aacaagacta cttccttatc tgggttagca attttagcac cgattgctgc tggaattcca
     9901 aagcccattg ttcccaaacc acctgaagtc actaactgac gttcattttg gtagggataa
     9961 tactgagctg tccacatttg gtgttgacca acgtctgtta ccacaatggc atctccattc
    10021 gtcaattcac caattcgttc aataactgct tgcggttgaa ccacacgctc tttcttatca
    10081 taagaacgaa cacgattctt gtctttagtg actttctcaa tccacttttc agtgttgttg
    10141 tgaactgttg gttctgctag caacatttgc aaggccttct tagcatctcc aactacagga
    10201 atgtctgcac tgataatctt gccaatctca gctgggtcaa tatcaatgtg ggcaacctta
    10261 gcattcttag cgaaagtctt aggattcccc gtcaaacggt catcgaaacg agaaccaata
    10321 ctaatcataa agtccgcttc cgtcatagca atatttgctg cgaatgaccc gtgcatgcct
    10381 cccattccaa gaaagagtgg gtgactcgtt gcaatcgttc cttgtcccaa aagactggtt
    10441 accactggaa tttgatagcg ttctgcaaat tcatttagtt ccgtagcagc ctcagcataa
    10501 ctaattccac caccagctaa caagactggc tttttagcct tggacaattg cttcaagatt
    10561 ttcttgattt gcatatcatt cggctcaaga gtcggctgat aacttggtaa attcacttct
    10621 ggtgaataaa tgaagtctgt ttctaaagca gatatgtctt ttggtaggtc aattacaact
    10681 ggccctggac ggcctgtagt tgcgatatgg acagcttccg taatgatacg cggaatatca
    10741 gctgtctcac gaacttggta attgtactta gtgattggca tggtaattcc cacgatgtct
    10801 gcctcctgaa aggcatcctt cccaatccct gctcgcgcca cctgacctgt aaagaccaaa
    10861 aggggaacgc tatcgctcat ggcatccgca atccctgtaa tggcatttgt tgctcctggt
    10921 ccactagtga cgacggcaac acccaacttt ccagttgatt tggcataacc ttcagcttca
    10981 tgcaaacaac cttgctcatg gcgccctaga atgtggcgaa tgcctttaaa attatatatc
    11041 gcatcataaa aaggcaaaac cgcaccacca ggataaccaa agatggtatc aactcctaaa
    11101 ttacgaagtg tttccaaaac taggtccgac cccgtcttag gagattctaa actgattttc
    11161 tccattattc ccctttcttt cctcttaaaa at
//
LOCUS       DAVKJJ010000059        11085 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000059 DAVKJJ010000000
VERSION     DAVKJJ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11085
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.059"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(381..1361)
                     /locus_tag="VW144_001745"
     CDS             complement(381..1361)
                     /locus_tag="VW144_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU9601158.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTPPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            complement(1677..3692)
                     /gene="recG"
                     /locus_tag="VW144_001746"
     CDS             complement(1677..3692)
                     /gene="recG"
                     /locus_tag="VW144_001746"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU9601159.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLNGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQVYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERETDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMIETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEVWQEDPEWRMIALHLEKKEHLD"
     gene            complement(3711..4814)
                     /locus_tag="VW144_001747"
     CDS             complement(3711..4814)
                     /locus_tag="VW144_001747"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU9601160.1"
                     /translation="MKASPHRPTKVLIHLGAIRQNIQQMGTHIPQGTLKWAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(4804..5172)
                     /locus_tag="VW144_001748"
     CDS             complement(4804..5172)
                     /locus_tag="VW144_001748"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU9601161.1"
                     /translation="MKMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            complement(5211..6242)
                     /locus_tag="VW144_001749"
     CDS             complement(5211..6242)
                     /locus_tag="VW144_001749"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9601162.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(6244..7275)
                     /locus_tag="VW144_001750"
     CDS             complement(6244..7275)
                     /locus_tag="VW144_001750"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9601163.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(7356..9869)
                     /gene="secA"
                     /locus_tag="VW144_001751"
     CDS             complement(7356..9869)
                     /gene="secA"
                     /locus_tag="VW144_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU9601164.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKKRYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(10063..10302)
                     /locus_tag="VW144_001752"
     CDS             complement(10063..10302)
                     /locus_tag="VW144_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601165.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKILLVFGILSILMALLSKKVSLFVFGLICCLSL"
     gene            complement(10330..10962)
                     /locus_tag="VW144_001753"
     CDS             complement(10330..10962)
                     /locus_tag="VW144_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9601166.1"
                     /translation="MKLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKVLAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
BASE COUNT         3116 a         2596 c         2120 g         3253 t
ORIGIN      
        1 aacatatata cggcaaggcg aagctgacgt ggtttgaaga gatttttgaa aagtattaac
       61 acctacagac aagcctgacg atagcaagaa ctaccctact cgataggtat cggcttttgc
      121 tttctgaaaa aaattatttt aagcaatttg acaaatctag caacaaaaaa ttctataaat
      181 ataatagatt gaaactagaa tagtacacat ttgtttctaa aacattgtta gaaatcgatt
      241 tgactgtcct gaccgatttg tcctgttctt atttcatttt actatatttc catgataaaa
      301 cgcatagtat aaagttttct taatcccctg atactatgcg tgtttgtaat ttttaagatt
      361 ttgtgcttag agtcgactcc ttattttaga tatttaaaag gaatctcact tccacagagc
      421 cagttgtaga cttggtcatt gacaaataca ttcatggctt cgtgagcata ctcaggcatg
      481 atgcgatagg ttttatcgca ggtcagacga ttataaatcg caaactgggt aatgggatag
      541 caaacatcgt cgtccaagcc cgtaatcatc ttaacctcac cttggatacg atgggcaaga
      601 tttttgacat cgatataggc aagggtcgcc atgatttcct cctctgtttc atggaagggg
      661 tcgtgaaact tgaaataacg gaaaagttcg tcgtaagcct cgctagtatt accaatctca
      721 atcacccgtc tgaagtctga caagaaggga taaatggcaa ctgttttctg aattcgagga
      781 ttgagcgctg ctgcaactag agctagagcc cctccttgtg aggcaccata gctagaaaga
      841 cgcttctcat caacctgaga cagactagca acaatttcga ccaactggta aatatccaga
      901 taaacatcct tataaaagag gtggtcccga ccttccatag caccacggat aatatgcccc
      961 ttcacggtat ttcctaaagg agaacgcaag ccgtcttgtg agtaacctga ctggccccgc
     1021 acatccatgg aaacaacacc gtaaccagct acggtgaagc ccagcatgtc ggcccagtcc
     1081 cagccacgtc ccatataacc atgaaaatgg aagattagtg gaaccttctc ctcactcttt
     1141 ggaagaacaa tgcgtgcata gacctttcct tccttgcttc cttcaaatgt taactcatag
     1201 cacttgactt gaggaatgtg gaaatctctt tcctccaagt ggtaggatgg aggtgtggaa
     1261 acatttttca cttccccatc ccagaaagca tcaaagtctt ccggaacctc atcccttcct
     1321 ctataggtct taatttcttc tagcaaagct ggatttttca tagcatgctc cttttatttt
     1381 gtaatcgtta ccacaactgt agcatatcta tgtaatcaat gcaagaaaga aggataaata
     1441 gaaagtgaat ttagattctt tacttttagg atgatgggct gaaagtagtt ttagaaacga
     1501 aaaagagcat ttcgctctct cttctgttta tactagtttt ttgcttcctt cttttggaaa
     1561 gtggtttggt aatttacttt aatcgttact gtcatatgag agtcctcgtt tctttttgat
     1621 gactttagta taagagccaa acctaaaaac tagcttaagt tttccttaga gaaagcttaa
     1681 tccagatgtt ctttcttttc cagatgaagg gcaatcatgc gccactctgg atcttcttgc
     1741 caaacttcta tagaactaat gtagctagca acctttcttg cttcttctaa aatcggaaaa
     1801 tcttcgataa tatcagccac ttggaactct ggaagtcctg actgtctggt tccaaaaatc
     1861 tcaccagaac cacgcatttt caaatcttcc tccgcaagga caaatccatt ggtcgtttct
     1921 atcatgatgc gcatgcggtc tttcccagaa tccgtcttgg gattggcaac gagaacagcg
     1981 taggactgct tgtccccccg accgacacga cctctaagct ggtgaagttg actgagaccg
     2041 aagcgatcgg catccatgat aatcatgaca gtcgcattgg gaacgttgac cccaacctca
     2101 ataaccgtcg tcgaaaccag aatatccgtc tctctctcct tgaaatcctg catgatctgg
     2161 tctttttcgt cactcttcat cctaccatgt agaagagcca cctctgcctt gcctgcaaaa
     2221 tgagtcgtca actcctctga taaggcaatg gcatttttca aatctagagc ttctgattct
     2281 tcaatcaaag gagagatgac atagacttgg gaaccttttt gaatttcccc ctctaaccaa
     2341 gtcaagacct gaggtagttg ctcatgtttg atccagcgcg tcacaatagg cttccgacct
     2401 gctggcatct ggtcgataat ggaaacatcc atatctccaa aggctgtgat ggcaagcgtc
     2461 cgtggaatgg gagtcgccgt catcatgagg acatctggat tgtcaccttt ttcccgtaaa
     2521 atacgccttt gccctacacc aaaacggtgc tgctcatcga taataatcaa accaagacga
     2581 gcatactcca ccccatcttg tatcagagcg tgagttccta taatcaaatc agcctcaccc
     2641 ttggcaatgg tctccaagac ctctctcttt tctgcagctt tcaaggaacc tgtcaagaga
     2701 gccagtttca aattgggaaa aaggttctgt aaactctcaa agtgttgctc tgcgaggatt
     2761 tctgttggta ccattagggc agcctgataa cctgctgtca ctgccgcaaa catggccaag
     2821 ccagcgacta ccgtttttcc actccccaca tccccttgta ggagacgatt catgtggtgg
     2881 tcggacttca tatcagttaa aatttcctgc aaactctttt cctgagcttg ggtcagggca
     2941 aaaggaagac ttactttaac tgctgtcact ttttcctgag accaattcag aaccagacca
     3001 cttccctgaa ctctattttc agacttgagc atctgcagct gcatttggaa ataaaagagt
     3061 tcctcaaact ttatacggcg aagagcctgc ttgtattctg ccaaatcctt tggaaaatgc
     3121 atagctcgga ctgcctgaca acgggacatg agtttgtatt tgtctagtaa agactggggc
     3181 agattttctt ctatcaagag gtccagtccc tgatcaaaag ccgtcttgat gaccttaacc
     3241 agactggcct gactgattcc ctgagccaga cgataaacag gctggaggtc atcttctacc
     3301 tgagccagaa ccttcatccc attcagacta gccttagcgc ggtcccattt tccaaagaca
     3361 gcaagggttg ctcccaactc tattttatca gccagatagg gctggttaaa gaaattcacc
     3421 gcaaaaacga cctctccctg cttgagacta aaacgcaggc gattgcgctt gaaaccataa
     3481 tactggacac tagcaggagt cactacctga ccagaaagaa ctgccttctc accgtcttcc
     3541 agctccagca cctgcttggt tttgaagtct tcataacgga aaggaaagta gagcaagaga
     3601 tcttgcaagt tttcaattcc tagtttggcg tatttttctg ctgactttgg tcccacacca
     3661 ggcaagacat gcaagggttg atgtagattc atgctccact cctttctttt ctaataatat
     3721 tctctcggaa tacggtcgct gaggaggcaa accacctcat agttaatggt tacgcggtag
     3781 gtcgctacct gagttgcagt gatttcctta tccccattgg agccaatcaa ggttaccttg
     3841 gttcctagcg gataaagctt aggcaatcga atagtgattt ggtccatcga aaccctgccg
     3901 acaattgggc aagcttggcc atctaccaag acagagaaat tttgcatgtc tcttgtccat
     3961 ccatctgcat acccgattgg cacggtcgcg atgacttgct cgctatccgc ttgataagtt
     4021 gctccatagc ccatgcaagc tcctgctgga actgtcttga catgaaccag agcagactcc
     4081 aaggtcaagg ccggtatcaa atcataaggc aaatccaaga ccgctccact tggattgagg
     4141 ccatacatgg catctcccat acgaaccgca ttgaaaatag tctctacatg ccaaagagtc
     4201 gttgcagaat tgctagcatg aaccagctct ggaacttcct tcatactagc taaaatagtt
     4261 ttaaaccgtt ctaactgggc attaaaatag tcatctgatt cctcatcagc agtagcaaag
     4321 tgggtaaaga ttccttcaac acgaacaccg tgttgttgta gcaaatcttg agcctgctca
     4381 acctcactgg cctctctaaa accaatccgt cccatccctg aatcaatctt gaggtggact
     4441 gtcaatccag ttaggtccac ttccttatct aagagtgctt gaatccactc cagtccagcc
     4501 actgtcaagg tgaagtcata ttctttagct agagcaacag cttcgatttc agaaactcct
     4561 aaaatgagga ttggcttgct gagtccagct tgtctgagtt caatggcttc atcgatattg
     4621 gaaacgcaaa agccatcaac atcatcttga attgccttgg caacggcaac agctccatga
     4681 ccataagcat tggccttgac cacagcccac ttgagcgttc cttgagggat atgagtcccc
     4741 atttgctgaa tattttgtcg aatagctccc agatgaatca gaaccttggt tggtctatgt
     4801 ggactagctt tcatgatttt cctccaaaat gacactggct gtcacaaact gatcggtgtg
     4861 gctgatagac agccaaatct ttcctgaaaa tggtgcctga ctaaaataag gcgccccacg
     4921 ttcattgttc aatacttcca aatcctgaaa accgagcttg ctaatgcccg ttcccatagc
     4981 cttggaaaag gcctccttag ccgaccagcg accagctaaa tattctattt gcctgcgtcc
     5041 tttgagactg gtgaagcgct ccatttcctg agcagtcagt acacgcttag caaatccttc
     5101 atgtcgtgta actgcgcttt ctatcgaagc caattcttcg atgtcaattc cgtgtccaac
     5161 tatcattttc atcaaaagga gttgagattc cccaactcca tccttttcac ttattttgtc
     5221 aaggtaacat aaatctcttc taccaaggcc tctgtatttt cccaacctag gcaagggtca
     5281 gtaatagagc aaccaaagat ctctggttgg ttttgacgac catctgctag gtaagattca
     5341 atcataaatc ctcgaaccgt ctttttaatt ttctcattcc aatcacgatt ctgcaaggtc
     5401 tggcgaacaa ttcgaatctg ctccatatat tgcttgcctg agttatcatg gttggtgtca
     5461 atgaggataa aaggattttc aagtcccatg gtttcatagc gttcaatggc ttggagtagg
     5521 ttttcatagt agtaattcgg catataattg ccatactcgt tgactgctcc acggaggata
     5581 acatgggcca aaggattacc tgatgtctca acttcctgcc catgataaag gaaggtttgc
     5641 ttgttttgag cagcatagat ggcgttaaac ataacaccca aatttcctga ggttggattt
     5701 ttcatcccta ctggtgcatc aatcccagaa gccacaaagc ggtgctcttg gtcttccaca
     5761 gaacgagctc caacggcatg gtagctgacc aagtcatcca ccaagatcag atttgacgga
     5821 taaagcatct catctgccgt tgttaaacca gtctctgtaa tcacgcggta gtgcaactgg
     5881 cgcacagcct gcaagccgtt aatcaggctt ggagccttag aagtatctgg ctggtgaacc
     5941 aatcctttat agccgtctcc attggtacga ggcttagcag tatacacgcg catgaccatg
     6001 aaaatcttat ccgctacctt cttttgcaag gcggataaac ggcgagcata ttccaagacc
     6061 gcctcttcat tatcagaaga gcaaggacca atcaccaaga gaatacggtc atcttcccct
     6121 gaaataatat ctgccaattc cctgtcacgg ctttccttga gtctcaaggc ttccgcagac
     6181 aattgggttt cagccttgat gacttccata tcgatttctt gacctttttc aataaatgcc
     6241 atcttattct cctaacgttt tgtaaatttc tctgacaaga gcttctgtgt tatcccaacc
     6301 caggcaaggg tctgtgatag acttaccaaa tacttctggc tcattttgtc gaccatcttc
     6361 cagataagac tcaatcataa aaccacgaac gaactgctta attttttcat tccaagcacg
     6421 gttaatcaag gtctggcgga caattcggat ctgttcaata tactgcttac cagaattgtc
     6481 atgattggta tcaatgatga taaaaggatt ttccaagccc attttctcat actgggcaat
     6541 ggtatcaatt aaattgtcat aatagtagtt gggaatattt tttccatact cattaagagc
     6601 accacgaaga atagcgtgtg aaagcgggtt cccagttgtt tctacttctt ttcctaagaa
     6661 aaggaaactt tgtttgtttt gagcagcata aattccatta aacatgacat tgagatttcc
     6721 agaggttgga tttttaaaac cagtagaaaa tcctgcccca cttgccacaa agcggtgttg
     6781 ctggtcttca actgaacggg caccaactgc catgtaagaa atcaaatcat ctacaagcgg
     6841 aaggttttca ggataaagca tttcatcagc agttgtcatc cctgtttctg tgatgacacg
     6901 atagtgaaga tggcgaacgg ctttgattcc attgataaga ctaggcgctt ctgtcgcgtt
     6961 aggctggtga atcaagccct tatagccatc tccgttggta cggggtttgg cagtataaac
     7021 acgcataacc ataaagatac gatctgccac ttcttcttgt aggactgcca aacgcttagc
     7081 gtattcaagg acagcttctt cgttgtcaga tgagcatggc ccgattacca agagaattcg
     7141 ctgatcttct ccacgtataa tggcttctag ctcttgatct cgctgtgatt tcctctccaa
     7201 agcctgacct tctaattttg acaaggcacg aacttcttct atatttattt tagagctttt
     7261 tgctgtaaat accataactc atctccttat aaagcaaacg gatacccagt aaaaatttac
     7321 ttttcacaag atatccgcct ctaaactatc tcattttatt gtcttttacc gtgacagttt
     7381 ttaaatttct taccagaacc acatgggcaa agttcattgc gtccaatctg gctcaaatcc
     7441 aaatcttctg gcatacttgc ttggtgagca gcgatattgc gagtcgctgt tgtactgata
     7501 tgacgttctg cctgtggtct ttcttgttca tgaatttgtg ctttcatcat caagcgtgtc
     7561 acatcaaact caatcgaacc aatcatatca ttaaacatac ggaaaccttc tgcctgatac
     7621 tcaacaacag ggttgttctg agcatagcca cgaagtccaa ccgcgttacg caattgatca
     7681 agggcatcga tatgatctgt ccacttgtta tccaccactc gtagaatcaa aactttttgg
     7741 aattctttaa ctgcttcttc atcgcgtagt tttgaaacct gactatcgta aaccttcaag
     7801 gcacgttgga aaagctcttc cttgatggcc ttatcagaca agcctgacaa gtcttccatc
     7861 gtaatagaat cttcaggaag caagttgtac ttagcaaagt tcaaaattgc ctctagtttt
     7921 tcatcttgtt tagcacgcgc atgaccgtca acgacacgtt caatcgtgcg tttgatcata
     7981 gactgaattt caggtgccaa gtcacgatct gcagtgatga catcgtaacg ttgagcatag
     8041 ataatctcac gttgttcacg catgacatca tcgtattgaa ggacttgttt acgggtatcg
     8101 tagttatttc cttcgacacg tttctgagct gcttcaacct gacgcgtcaa catgcgagac
     8161 tcaatcgcct cttcagacat gttgagacgt tcaaagattc ccttcaagcg ttcagaacca
     8221 aaacgtttca tcaaatcatc ttcaagagat aggtagaatt gtgactcacc tggatctcct
     8281 tgacgacctg aacgtccacg aagctggtta tcgatacgac gactttcatg acgttctgta
     8341 ccaataacac aaagtcctcc aagttcacga acaccttcac caagcttgat gtcggtacca
     8401 cgacccgcca tgttggttgc gatggtaacg gcaccacgtt gaccagcatt catgatgatt
     8461 tgggcttctc tatagtggtt tttggcattc aagacttcgt gaggaacacc agctgcgacc
     8521 aatttcttag aaatgtagtc actagtttca accgctactg taccaaccaa gacaggttga
     8581 cccttttggt aacgagcctt aacgtcttcg acaaccgctt taaacttaga ttcgatactt
     8641 gcataaagaa ggtctgagtg gtcaatacgt tgaacaggac ggtttgttgg gattggaata
     8701 acacgaatgt tgtagatttc acggaattct tcttcctcag tcttacctgt acccgtcata
     8761 ccagacaatt tcttgtacat acggaagagg ttttggtacg tgattgaggc agatgtcttg
     8821 gtttcatcct ggattggcac accttctttg gcttcaatag cttggtgcaa tccatcagaa
     8881 taacgacgac cttccatggt acgacctgta aattggtcga caatcaagat ttcttgctct
     8941 tcgctcacca catagtcaat atcgagaagc atgatgtagt tggcacgaag ggcgttatcg
     9001 ataaagtggg tcaaagccac gttttcgatg tcatagaggt tttcaagttt gaagtagctt
     9061 tcagccctgt caatccctga atcagacaaa ccaatagtct tagactgcac atcgatgatg
     9121 tagtcatctt tgttcaaaga ttttacatag tggtctgcca tgtgatacaa ctgactggtt
     9181 tcaaccgcat tggcacctga tacaatcaaa ggtgtacgag cctcgtcaat caagatagag
     9241 tcaacctcat cgaccaaggc atagttaagc ggacgttgta ccatgttttc agcgcgaacg
     9301 accatgttgt cacgaaggta gtcaaatccg atttctgagt tagttgagta agtaatatca
     9361 cactcatagg cttctttttt ctccattgga gatttggtag ccaagttaat ccctactgac
     9421 aaaccaagcc aagagtacaa ttcacccatc tcagtcgcgt cacgttctga caggtattca
     9481 ttaaccgtaa ctacgtgaac ccctttacct gaaagggcat tgaggtatac cggcatggtc
     9541 gcagtcaagg ttttcccttc ccctgtacgc atctctggca cgtcaccatg gtgaagaaca
     9601 atccccccca tgacctgaac cttataaggg aagagaccta ggacacgttt ggcaccttca
     9661 cggacaaccg caaatgcttc gtaaagcaat gaatccagtg attctccatt ttgataacgt
     9721 ttcttaaatt caactgtttt tgcttttagt tggtcgtcag tcaaagcagc catttggtct
     9781 tcgtatttga aaaccttgtc agccatcttt tccagacgac ggatttctcc tttatcattt
     9841 tcgataattg tttttaaaat attagccatg tttttcctta ctttaaattc cgaatatttt
     9901 agaatgttcc tttaatttta gcacaattac tgattatttt caaggaagaa tccccatttt
     9961 gaaaaggaaa aagaggttag ttttcaagct aacctctccg acttttatga ctgtttaatt
    10021 gccaaaaatt ggcaaaatgg caaatgtgat aaataagtta attcaaagag aaagacagca
    10081 aatcagtcca aaaacaaaga gactgacttt cttggacagc aaggccatca aaatggacag
    10141 aattccaaaa actagcaaga ttttctgtat gatgaagatg tcaatcctta cccccagaat
    10201 cgctctatcc caagggataa agaagaaaga cagccagatc agcagaacta aaccaatata
    10261 aaccttggaa tagcgtgtaa taaatgattt tagatgtgcc acaagccatc tcctaggatt
    10321 gtatatcctt tacatttgta tatgtccatt ttcgacaact aatacatctg tacaaaggtc
    10381 tgaaatatct tccgatatgt gcgacgtcag gataaccaga ccattttctt ttttcaggta
    10441 agataaaatg acctgtttgg ttaacctcac actcttctca tccaaagcat tcataggttc
    10501 atcgagaaag agtatagaag gagaggaaat aaaggcttga atcaaggcca ttttttgctt
    10561 tgttcccaag gataaatgcc ggtattcaac gtcttcaaat tcctgtaaat catagtattg
    10621 aatccaatag gcaattcttt tttccgtaac tttagatgaa aaatacctta acagttccaa
    10681 attttctctc agggataaat gagataaaaa ctcgactttt tcaattaaaa ttcctgcatc
    10741 ctgaatataa tgatttttta tcccgtaaac tttaccatct tgaagaactt ttcctttgtc
    10801 aagcttaata taaccagcaa gtacctttaa aagaaccgtc ttgccagatc cattatcacc
    10861 tttaagtcca taaatttttc ctgattgaaa caccaaattc aactgattta aaataggtct
    10921 tttcccataa attttctgtc catttataat ttcaagtttc atcgtttatc ctttcttaaa
    10981 tttctcttcc aagcgactaa caatcaacat aactaataag cttaagagta gtaaatacac
    11041 tgttacaata tgatccatca tccattcttg tcccacaaat gctaa
//
LOCUS       DAVKJJ010000060        10812 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000060 DAVKJJ010000000
VERSION     DAVKJJ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10812
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.060"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            1..737
                     /locus_tag="VW144_001754"
                     /pseudo
     CDS             1..737
                     /locus_tag="VW144_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            637..1083
                     /locus_tag="VW144_001755"
     CDS             637..1083
                     /locus_tag="VW144_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9601167.1"
                     /translation="MRKHGLISKSTSKRYYQVAIPFTRLFCPALVSIDYINCLSETTD
                     KNWHKSDRIFVTNIGKPVHSSILSKSLQRANERLKKPIPKHLPPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTQVTKNMKDEAINVLDKVMKKIF"
     gene            complement(1221..2525)
                     /gene="eno"
                     /locus_tag="VW144_001756"
     CDS             complement(1221..2525)
                     /gene="eno"
                     /locus_tag="VW144_001756"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU9601168.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2689..3135
                     /locus_tag="VW144_001757"
     CDS             2689..3135
                     /locus_tag="VW144_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEU9601169.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(3132..4253)
                     /locus_tag="VW144_001758"
     CDS             complement(3132..4253)
                     /locus_tag="VW144_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEU9601170.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENRYKIPEGVQIHILADVVSPLCGYQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDK
                     KVSDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQ
                     AVFSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(4262..4591)
                     /locus_tag="VW144_001759"
     CDS             complement(4262..4591)
                     /locus_tag="VW144_001759"
                     /inference="COORDINATES: protein motif:HMM:NF012905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEU9601171.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAELGIAFCAKEVLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(4536..4907)
                     /locus_tag="VW144_001760"
     CDS             complement(4536..4907)
                     /locus_tag="VW144_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEU9601172.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            complement(4966..6399)
                     /gene="glgA"
                     /locus_tag="VW144_001761"
     CDS             complement(4966..6399)
                     /gene="glgA"
                     /locus_tag="VW144_001761"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEU9601173.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGLERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(6396..7535)
                     /gene="glgD"
                     /locus_tag="VW144_001762"
     CDS             complement(6396..7535)
                     /gene="glgD"
                     /locus_tag="VW144_001762"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEU9601174.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAEMFNMSTDIFVVDTPWLIEHLEEEAKKEHPKK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GKGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(7525..8667)
                     /locus_tag="VW144_001763"
     CDS             complement(7525..8667)
                     /locus_tag="VW144_001763"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEU9601175.1"
                     /translation="MKNKMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVSLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGAVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8657..10585)
                     /gene="glgB"
                     /locus_tag="VW144_001764"
     CDS             complement(8657..10585)
                     /gene="glgB"
                     /locus_tag="VW144_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEU9601176.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAIVTELPDKKWRDGLWLARRKRWGFVERPVNIYEVHAGSWKRNPDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDNAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3311 a         2368 c         1916 g         3217 t
ORIGIN      
        1 ctggttaaag ctaaaagaga aaacaggaga gctaaaccac caagtaaaag gaacaaaatc
       61 aagaaaagtt gatagagata gacttaaaaa ctatcttact gacaatcccg acgcttattt
      121 gactgaaata gcttctgaat ttggctgtca tccaactacc atccactatg cgctcaaagc
      181 tatgggctac actcgaaaaa aagaaccaca cctactatga acaagagcca gaaaaagtag
      241 ccttatttct taagaatttt aatagtttaa agcacctagc acctgtttag attgatgaaa
      301 caggattcga tacttatttt tatcgagaat atggtcgctc attaaaaggt cagttaataa
      361 gaggtaaagt atctggaaga agatatcaga ggatttcttt ggttgcaggt ctaacaaatg
      421 gtgagttaat cgttccaatg acttacgaag agacgatgac gagcgacttt tttgaagctt
      481 ggtttcagaa gtttctctta ccaacattaa ccacaccatc ggttattatt atggataatg
      541 caagattcca tagaatgggt aagttagaac ttttatgcga ggagtttggt cataaacttt
      601 tacctcttcc tccctactcg cctgagtaca atcctattga gaaaacatgg gctcatatca
      661 aaaagcacct caaaaaggta ttaccaagtt gcaatacctt ttacgaggct cttttgtcct
      721 gctcttgttt caattgacta tataaattgt ctaagcgaaa caaccgataa gaattggcac
      781 aaaagcgacc gtatttttgt taccaatata ggaaaaccag ttcatagttc tatcttgagc
      841 aagtctctcc agcgagcaaa cgaacgcctt aaaaaaccaa ttcccaaaca tctgccccct
      901 cacatcttca gacacaccac tattagcatc ttatcagaaa ataaaattcc tttaaaaaca
      961 atcacggaca gggttggtca ttccgactct gaagtcacta cttccatcta cacccaggtc
     1021 acaaagaata tgaaagatga agcaatcaat gtactggata aagttatgaa aaagattttt
     1081 taaaaagttt tgtccctttt ttgtcctcta aatacaaaaa tagcccttcg gataaaatcc
     1141 gaggggctag aaacgttgtt aaatcaacgg ccgaactttt gaatttcatg gttcgggata
     1201 aaatagttca ctgaactatt ttatttttta aggttgtaga atgatttcaa tccacgatat
     1261 tcagctactt caccaagttg gtcttcgata cgaagcaatt ggttgtattt agcgatgcgg
     1321 tctgtacgtg aaagtgaacc agtcttgatt tgtcctgcgt tagttgcaac tgcaatatca
     1381 gcgattgttg aatcttcagt ttcacctgaa cggtgtgata caacagcagt gtaaccagct
     1441 tctttagcca tttcgatagc ttcaaaagtt tcagtaagag taccgatttg gttaactttg
     1501 ataaggattg agttagcagc accttcttgg ataccacgtg caaggtagtc agtgtttgtt
     1561 acgaagaagt cgtcaccaac aagttgtact ttcttaccaa gacgttcagt aagagctttc
     1621 caaccatccc agtcgttttc atccatacca tcttcaatag tgatgattgg gtatttgtta
     1681 accaattctt caaggtagtc gatttgttct gcagatgtac gaacagcagc accttcacct
     1741 tcaaatttag tgtagtcgta aactttacgt tctttatcgt agaattctga tgaagcacag
     1801 tcaaatccga taaatacgtc tttacctggt acatatccag cagcttcaat cgcagcaagg
     1861 atagtttcaa caccatcttc agttccttcg aaacgaggag cgaatccacc ttcgtcacct
     1921 acggcagttt ccaaaccacg tgatttaagg attttcttaa gagcgtggaa gatttcagca
     1981 ccgtaacgaa gggcttcttt aaatgttggc gcaccaactg gcaagatcat gaactcttgg
     2041 aaagcgattg gagcgtcaga gtgagaacca ccgttgatga tgttcatcat tggagttgga
     2101 agaactttag tgttgaatcc accaagatag ctgtaaagtg ggatttcaag gtagtcagca
     2161 gcagcacgag ctacagcgat agacacaccg aggattgcat tcgcacccaa tttacctttg
     2221 ttaggagtac cgtcaagtgc gatcatagca cggtcaatag cttgttgatc acgtacatcg
     2281 tagccaatga tagcttcagc aatgatgttg tttacgttgt caacagcttt ttgtgtacca
     2341 agaccaccgt aacgagattt gtcaccgtcg cgaagttcaa ctgcttcgtg ttcaccagta
     2401 gaagctcctg atggaaccat accacgtccg aaagcacctg attcagtgta aacttctact
     2461 tcaagtgttg ggttaccgcg tgagtctagg acttcgcgag cgtaaacatc agtaataatt
     2521 gacatttttt actctcctta tgagttaaat tttttacacc tctataatac cttaaaaccc
     2581 ctcctttttc aagaaaaaac gttatctttg tgcaactttt ccttaacttt ataaagtaat
     2641 cgctttcttt tgtctgtttt attctaactt ttatgatata ctgttttcat gacagattta
     2701 tcaaaacaat tacttgaaaa agctcatggt gggttaaaaa taaatccgga tgagcaaaga
     2761 cgctatcttg gtacttttga ggaaagagtt cttggatatg tagatattga cacagcaaat
     2821 agccctcagt tagaaaaagg ctttttattt attttagaaa accttcagga aaaagcagag
     2881 ccactatttg tgaagatttc accaactatc gaatttgata agcaagtttt ctacttaaaa
     2941 gaagcaaaag aaactgatag tcaagccacc atagtatctg aagagcatat tacttctcct
     3001 tttggcctgg ttattcatag caatgcacca gttcaagtag aagaaaaaga ccttcgactt
     3061 gcttttccaa aactttggga agttaaaaag gaagaaccag ccaaaacatc cttatggaag
     3121 aaatggttta gctaaatctt gcacatattt aataagtgcc caatattggc agccgtgcgc
     3181 tccagataga gactggcatt tttcaaacta tcttctaaag gttcactttt ctccaaaata
     3241 gaaaagacag cttggatatt ttcaaatggt aggggaggta aatcttcagc aagactaccg
     3301 caaatagcaa taacaggaac tccaacaggg gttctttttg caacacctat aggcgctttc
     3361 ccagcaaagc tttgactgtc aagtcttcct tctccaacaa caaccaagtc agcatctgaa
     3421 actttcttat caaagttgat taagtccaag caggtatcaa ttccagacac gatacttgcc
     3481 tgagcaaagg cacacaaacc accagcaagg cctccacctg ctcctgctcc tgctccttta
     3541 atttctaatg ttgcaggtga gaatttttca taaaaatctt ggatcgcctg atctacgact
     3601 gcaaacatag tcggatgtag acctttttga ttgccaaaag tgtaagtcgc accttgataa
     3661 ccacataagg gactcacgac atctgctaaa atatgaattt gaacaccttc aggaatttta
     3721 tagcgatttt ctgttgaaac agaagctaag tttaataagg attgaccgga agcaggcaag
     3781 acatttccat ccctatcata aaattgataa cctaaaccag cagcaatccc cagtcctcca
     3841 tcattactgg ccgtgccacc aacaccgata taaatatctt taatcccttt agaaatgaga
     3901 tggagaatca actctccaat accacaagtt tggatttgaa gtggatttcg tttctctagc
     3961 ggaatttttc caagaccaac caagtcagct acttcaaata gtgccagttc ccctttttga
     4021 aaatagcgca tggcttcttt ttgtccaaaa gggtctgtca cttggatcca tttttctttt
     4081 aggtcaagag aatgtcggat agcatctaca gtaccttctc ccccatcacc aacagggcag
     4141 aggagacatt ctacatctgc tatcgattgt tggaagcctc tttttattgc ttcagctacc
     4201 tgttgagctg tcaagctttc cttaaacgaa tccggtgcaa ttacaatctt catattttcc
     4261 ctcattctaa acagtcaatc aaaggaagaa cttctaaaaa atccctcttg tcaacatgat
     4321 gtggtatttc ttttttgagc acttctttgg cacaaaaggc gattcctaac tccgccgact
     4381 tcaacattaa tagattattg accccatcac cgattgccac cgttctttct ttagaaagtt
     4441 ttagtttctt tctccatttt tccagagttt cttttttgac ctggggactt ataatttgtc
     4501 caactaattt tcctgttaaa agaccttctt tgacttcaag ctggttggca gtgaaatagg
     4561 caataccaag ggattttgct aatctctcca actattggtg taaatccacc agacaccaga
     4621 ccaactagga tgccattctt ttggagaata gagatgaact ctgggacatt tagcgataga
     4681 tgaattgagt tgaagacgtt atcaaagacc aaaataggaa gaccttccaa caaggacact
     4741 ctttttctta aactgctttc aaagaccaac tctcctcgca ttgctcgact tgtaatctgc
     4801 gaaatttccg cctcatgacc tgcctctctc cctaaaagat caatcacttc ttctaggatt
     4861 aaggttccat ctacatccaa aacacacaag ccttttactt gagacatcag ttctcctctc
     4921 taaacagcct aaaaatcgta tgaagtcatc atacgatttt atctattaat taactaaact
     4981 atggtacaag tcaaggtatg acttgcaggc tgtatcccat gagaagtcac tctccatagc
     5041 ttgtttttgt aggtttctcc aaatgtctgg atggtttcta tacaagtcca atgctgtttg
     5101 gaaagtccaa tttaaccaat aaggagatag attgtcaaag ctaaagccag taccgcttcc
     5161 ttcgattgga ttgaaagcgc gaactgtatc tcgcaagcct ccaacttcat ggaccaatgg
     5221 caaggttcca taacgcatag ccatcatttg agacaagcca cacggttcaa aacgacttgg
     5281 catgaggaag aggtcacaag cagcgtagat ttcctgagca agtttgacat caaaagtgat
     5341 atttgttgat agcttgtctg ggtaaatctg agcaaaccat gagaaagctc cttcaaaggc
     5401 tggatcgcca gttcccaaaa gaacaatctg aacatcttct tgcaagatat ggtgaagact
     5461 ttcgaccacc acatcaaaac ctttttgacg tgtcaaacga gaaacaattc ccaccagtgg
     5521 aacgtctgct ctaacaggca agccaactct ttcttgcaat tttgccttat ttttggcttt
     5581 cccagacaaa tcttcctgat tgaaatgata gtctaaaaga gcatccgtct gaggattata
     5641 aagatcagca tcaatcccat tcacgatacc agatacttta ccagactcca ttttaagaat
     5701 ctgatccaaa ttacatccaa actgactagt cataatttca tgagcatagc taggtgaaac
     5761 ggttgaaaca cggttcgcat agagaatacc tgccttcatc cagttcagac agttgttcca
     5821 tcgaagggtg ccatcagcgt aacgttcaag gccaactcca aacaaatcac ccaacattcc
     5881 ttctgaaaat tgtccttgga attctaaatt atgaatggtt aaaactgttt caatgtcctc
     5941 ataggcttga atccaacggt atttttcctt caacaagaaa ggaatcatag ctgtatggta
     6001 gtcatgaaca tggagaagat caggaataaa gtcaatcctt tccatagcct caatggcagc
     6061 cagttggaaa aaggcaaagc gttctccgtc atcaaaatca ccgtaaacat gaccacggaa
     6121 gaaataatat tgattgtcaa taaagtagaa ggttacacca tttaatactg ttttcttaat
     6181 tccacaatac tgtctgcgcc aaccaacgct cacctcaaaa tgaagcacat cttcaatctg
     6241 atttccaaat ttagcctcta ccatatcata gtagggtaaa atcactgcaa cttcgtgccc
     6301 agcttttacc agtgattttg gaagagcgcc aatgacgtct cccaaaccac ctgtttttga
     6361 aaaaggtgca ccctctgctg ctacaaataa aattttcatg aatgaatatc ctctgttact
     6421 ttagcacctt tcttaaccac aactggatgt tctgcagttc ctcgaatcac aacaccaggc
     6481 tcaacttcaa cccctttgtc caagatagca tattcgacct gagccccttt tccaataaca
     6541 acacgaggga agagcaggct atctttaacc aagctatcct tatggacatg aatattacgt
     6601 gatagaacag aattagctac ttgaccttca ataatactac cagaggcaaa ctgagaagtg
     6661 cttaccttag atgtattagc atagtaagtt ggctcttcgt ttttgacctt tgtataaatc
     6721 ttttggtttg gtgagaaaag agaatagaat ttttgtgatt caagcatatc gatattcgct
     6781 tgataataag atttaacaga gtgaatattg gctagatagc ccgtgtactc gtaggcgaaa
     6841 gctccctctt ttacagccaa atcccgtaaa acatagcgca atttctttgg atgttctttt
     6901 ttagcttctt cttccaagtg ttcaatcaac caaggtgtat caacgacaaa gatatctgta
     6961 gacatattga acatttcagc tgttgacttg ctatcaaaga gtttatgaga aagaacatgg
     7021 tctgtttcat ctacatccaa gattgcattt acttctgaaa tatctttctt agctagtttt
     7081 ttataaacta cagtgatagg ctcttttgtt gtactatgta ggtggaaaac ttggttcaaa
     7141 tcaatgttaa taagaacatc gcagttgagg gcaaccgttt ggtttgagcc agaacgtttc
     7201 aaataagtaa gaagctgttg gtagtattct tttccaactg tactactttc tacacgggta
     7261 ttgtaaattc ctagatagta atgactaaga agggttgata agccccactc gcgtcctgaa
     7321 cgaatatggt caaatactga gctgatatta tcctgctgga aaataccaaa gacactacga
     7381 acacctgcat tagcaaggct tgaaagtggg aagtcaatca aacgatattt cccaccaaat
     7441 ggcaaacttg ctactggacg gtggtccgtc aatgtcgaca tattgtgaaa accaactgta
     7501 tttcctaaaa tggcagaata tttatcaatc ttcatctgtt gctaccccca ctacttcatt
     7561 atatcctaca acttgtactt catctgttcc atcaatttcg acaccgtcag aaataatcgc
     7621 accttcacca ataatggcac gtttaatctt agctccttga ccaatgatag ctccactcat
     7681 gataactgaa tcaaggactt ccgctccttc gcgaacttgc gcgcctgttg aaaggataga
     7741 atgtttaaca gctccatcaa cgaaacatcc gtctacaact aatgagtctt ccacatgagc
     7801 atttgccccg aggaagtttg gtggtgaaat caagtttctt gagtaaatct tccattgacg
     7861 gttacgacta tccaaggcat tttctggaga aatatactcc atgttcgctt cccaaagtga
     7921 ctcaatagta ccaacatctt tccaataacc actaaattcg taagcataaa cactttcacc
     7981 tgactcaagg taatttggaa tgacattttt accaaagtct gacatgccaa ccttgctctt
     8041 ttcagcagcg actaacatat tacgaaggcg ttgccaatca aaaatgtaga ttcccataga
     8101 agcttttgta gatttaggtt gagctggttt ttcttcaaat tcaacaatac gattgttagc
     8161 atctgtgttc atgataccaa aacggcttgc ttctttaaga gagacgtcta aaactgctac
     8221 tgtcaagctg gcattattat ccttatgaga ctggagcata tcatcatagt ccattttgta
     8281 gatgtggtcc ccagacaaaa tcaagacata ctcaggattg acactgtcga tatagtcgat
     8341 attttggtaa atagcgtgac tagttccctc aaaccaacga tttccttcac ttgcagaata
     8401 aggttgaaga atagagacgc ctgaattaat accgtctagt ccccagcttg aaccattccc
     8461 aatatggttg ttgagagcaa gtggttgata ctgtgtaacg accccaacat tatgaatccc
     8521 tgagttggca cagtttgata gggcaaagtc aatgatacgg tagcgcccac caaattgcac
     8581 agctggtttt gcgatgcttt gagtgagttt accgagacga gttccttgcc caccagcaag
     8641 aatcaaagct aacattttat ttttcatttt ctactccttt ttggttttta tttgtgacgg
     8701 ttttagtaga tttcaagcga cgtttgattt tccatacact tgctcccata gccggtagag
     8761 taaaggttaa ggtctgctca taatctttcc ataggccttc ttgcgtttga acagtttgat
     8821 tatgttcttt ccaaacgcct ccccactctt ccaactcagt attccatact tcttcgtaaa
     8881 ttcctgcaac gggtagtccg attgtaaaat ctttccgctc aacaggtacc atgttaaaga
     8941 tacagactaa catttctccc tttttaccct tacgaataaa ggaaagaaca ctctggtctc
     9001 gattatccgc atcaatgatt tcaataccat catagctggt atcgatttcc cacagacagc
     9061 gatgatcttt gtaaaactgg tttagctgag aagtgaaata cttcatctta gcattcattg
     9121 ggtcttctag gttagaccat tccaactgtt cttcagattt ccattctagg aattgaccgt
     9181 attcgctacc catgaagagc aatttcttac cagggtgaca aatttggtac gtatagagat
     9241 tgcgcaagcc tgcgaattga ttgtaacgat ctccccacat cttatgcatc atactcttct
     9301 tgccatgaac cacttcatcg tgcgagaatg gcaagagata attctccttg aaaacataca
     9361 taaagctgaa agtcaccagg ttaaagtcat atttacgata aatcggatct tcttcgtaga
     9421 aacggaggat atcattcatc cagcccatgt tccatttgta gtcaaatcct agaccaccaa
     9481 tctctttcat tcccgtaatc ttggtcgcag acgaactttc ttctgcaatc atcatcacat
     9541 ctggatattc taacttaata acttcattca agcgttgaag aaaataataa ccttcatagt
     9601 taagatttcc gccatcttta ttaggtgtcc atggagcatt atcatagtcc aaatagagca
     9661 tgttgctaac agcatccaca cgaataccat ccaaatgata gacatcaatc caatgcttaa
     9721 tgcaagaaat taagaaggac tggacttcat tttttccaag gtcaaaatta agggcacccc
     9781 aaccatggtt atgagcctta ttatggtctt ggtattcaaa agtcggtgtc ccatcataat
     9841 aggctaaggc atcatcgttg atggtaaagt gacctggtac ccagtccaca ataaccccaa
     9901 tattatgggt atgacactcc tcgacaaaat cttgaaactc ctctggtctg ccataagcat
     9961 gctctaaagc gaagtaaccc ataagctgat acccccaact caagcccaaa ggatgggaca
    10021 tcaagggcat aaactcaata tgagtatagt tcatttcaac gagataagga atgagttcat
    10081 ccttgagctg ggcaaaacta taaggactgc catcaggatt tctcttccaa gagccagcat
    10141 gaacctcgta aatattgaca ggacgttcaa caaagcccca acgttttctt cgtgccagcc
    10201 aaagtccatc cctccatttc ttatcaggaa gctctgttac gattgcccct gttcctggac
    10261 gagcctcata cctgacagca aaagggtcaa tcttcatcag ttgatgacca ttttgacgtg
    10321 tgacatgata tttgtaaata tgcccttctt gagccatatt ggtaaagact tcccagaccc
    10381 caaagtcatt tcttaccatt ggaatctgat tttcaatcca gttggtaaaa tcaccaacca
    10441 agtgaacagc ctgagcatta ggtgcccaaa cacggaaggt atagccatgc tctccattta
    10501 gttcttccct atgtgctcct agataatgtt ggagataaaa attttcaccc gtcataaagg
    10561 tttttaatgc ttctctatta tccatatact ccccttctcc tgtaagcgtt ttctatcttt
    10621 ttattatact acctttttag agaagattca agtaaattac tatacttctt taattatttt
    10681 gaaaatctac aacaagttca cttactcgtt caattgtaaa tcaatatttt ttcaaaaaat
    10741 tgcgaaaacg cctttctttt tctactatag tgaaatgaaa taaaacatgc gcaaatcgat
    10801 caggacagtc aa
//
LOCUS       DAVKJJ010000061        10773 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000061 DAVKJJ010000000
VERSION     DAVKJJ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10773
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.061"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(54..470)
                     /locus_tag="VW144_001765"
     CDS             complement(54..470)
                     /locus_tag="VW144_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9601177.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(482..1177)
                     /locus_tag="VW144_001766"
     CDS             complement(482..1177)
                     /locus_tag="VW144_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9601178.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPQEMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            complement(1371..3872)
                     /locus_tag="VW144_001767"
     CDS             complement(1371..3872)
                     /locus_tag="VW144_001767"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU9601179.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTS
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVVDYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVACLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            complement(4327..5415)
                     /locus_tag="VW144_001768"
     CDS             complement(4327..5415)
                     /locus_tag="VW144_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEU9601180.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(5433..6101)
                     /locus_tag="VW144_001769"
     CDS             complement(5433..6101)
                     /locus_tag="VW144_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU9601181.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLVLAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            complement(6120..8558)
                     /locus_tag="VW144_001770"
     CDS             complement(6120..8558)
                     /locus_tag="VW144_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEU9601182.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKAALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            complement(8709..10007)
                     /locus_tag="VW144_001771"
     CDS             complement(8709..10007)
                     /locus_tag="VW144_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9601183.1"
                     /translation="MAKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSMTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFVAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLAMSTMVYFPFFKIQDRLAYQNEIKQS"
     gene            complement(10024..10332)
                     /locus_tag="VW144_001772"
     CDS             complement(10024..10332)
                     /locus_tag="VW144_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9601184.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            complement(10378..10698)
                     /locus_tag="VW144_001773"
     CDS             complement(10378..10698)
                     /locus_tag="VW144_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9601185.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
BASE COUNT         3301 a         2346 c         1766 g         3360 t
ORIGIN      
        1 actatataat actagaccat ccttaaattt tgcttaagaa aaaacaagtt ttgttatttt
       61 ttacgattca tccaagtcat ccctatacaa ttataggtgg ataagatttc aaagcccata
      121 gaacgataga agcccaaggt tctttctgtc tcttctgtca ccagctggac ttgataggca
      181 tctttgtaat cctctaaagc ctctttcatc aaggcactac caattccttg acgctgatag
      241 attggtaaaa cgattaaatc ctgaaccaat actgatgaga atccatctcc aaccaaacga
      301 atcaagccca ccacagcatc accatcaagt gccagataaa ttactaatga atgagataag
      361 gcctgctcca gcatctctgg ttgatgggta taatttgtcc aaccgacagc ctgatagaga
      421 tgtaaaacat cctctagctt gacaatttct tgctttttaa tagtaatcat ctcaacacct
      481 cttaaagttc tctcaagctc ttgtactgct gtccattttt atcaaaattt tcaggacgca
      541 accatgtttc caaacgatct ttgacttgag gccagtcctt atcaatcata gacaaccaat
      601 ccgtatctct cgtacgtccc ttataaacca ctgcctgacg gaaggttcct tcataaataa
      661 agctcaaacg ctccgcagct cgtctggatg gcagatttaa ggcatcgcat ttccactcgt
      721 agcgacgata gtttagctcc tcaaaaacat agcgagctag gagatactga gcttcagttc
      781 ctatccgtgt accccttagc tctggagaaa aagtgactgc tccgacttct attactcggt
      841 tattctggtc aatgcgcatg agagaaaaag atcccaaagc cttaccagtc gccttgtcta
      901 tgattgcata gtaaaaacgg tccttacgag ccaacatctg atgtaaaagg ctaaccagct
      961 cccccatatc tgctactggc tcctgaaaaa ggtaggtcca catctcctga ggcgtatcag
     1021 gcccataaac agctagcaaa tcctccgcat gcttttccac tgaaagagcc tctatcctag
     1081 cataacgccc ttctaagaaa tcaatagaag gcagttcacc tggtgtataa ccttccattg
     1141 actccccaat catttgacca tattcattta ctggcatagc tttctccttt tttcactttt
     1201 caaactttat agttgcccat agtataccat actttcataa gaagattcaa aaaatccccc
     1261 agattctact ctgaaggatt cctaccttat ttcacaacga tattaacgag tttagttggc
     1321 tcaaacagtt tgggaaactg tttgaggttg gaaatcagca aataaattgg ttattttaca
     1381 acaatattaa ccaatttatt tggtacacta atcactttca cgatttcctt accgtcaatt
     1441 tctgctttaa ctttttcatc agctaaagcg atttcttgta attcttcacg tgatagatct
     1501 ttagcaacca tgagtttggc acggactttt cctttgattt ggacgacaat ttcaatttca
     1561 tcttcaacca atttgctttc gtcccaagtt ggccaagcta cataagagat tgactcacct
     1621 gtttctgcga ctgtttgcca gagttcttct gccaagtgag gtgcaaatgg tgcaatcaat
     1681 tgaataaagc ctttggcata gtcaacataa agcttatctt ccttgttagc agcattgaca
     1741 aagaccataa gttgggcaat agctgtgttg aatttgagag actcaatttg ctcagtaaca
     1801 gctttgactg tttcgttgta agccttgtca agagcaccat tgttttccgc aaggatttct
     1861 ttacttgtaa tcaaacggta aactcggtca aggaacttac ggcttccttc caaaccttct
     1921 tctgaccaag caatcgaagc atcgagtggt cccataaaca tttcataaac acgaagggta
     1981 tcggcaccgt attgttccac cacatcgtct gggttaacaa cgttcttgag cgatttagac
     2041 atcttggctg gcgcttgctc caactcttcc cctgtttcta catggaagaa ggaaccatca
     2101 cgtttttcaa ccttgtcggt tgccacaaga gcaccacggt ggtcacggta gcttgttccc
     2161 aaaatcatcc cttggttaaa gagtttttgg aatggttcct tagtcggaac aacaccgagg
     2221 tcatagagga atttatgcca gaaacgagca taaagcaagt gaagtacagc atgttccgca
     2281 ccacccacgt agatatctac tggcaaccat tgtttgagga ggtcctcatc agccaatttc
     2341 tcagtattgt gcgggtcaat atagcgaagg tagtaccagc ttgaaccagc ccattgtggc
     2401 atggtgttag tttcacgacg acctttgaca ccatcttcac gagtcacttc aagccagtct
     2461 gtcaagttag ctagtggact ttcaccggta cctgaaggac ggatatcctt ggttacaggc
     2521 aagacaagcg gcaattcagt ttcaggaaca gctgttgaag ttccatcttc ccaatgaatg
     2581 attggaattg gttcacccca gtaacgttga cggctaaaga gccagtcgcg gaggcggtag
     2641 gtaaccttct cctgaccaca gcctttttct tccaagcaag ccacaatctt ggcaatagcg
     2701 tcttctttgt tcaatccatc taggaagtct gagttgacat gcaggccatc ctctgtgtag
     2761 gcagcttctt cgacatttcc accttcaagt acttcgacga ttggaaggtc aaattgtttg
     2821 gcaaattccc agtcacgttg gtcgtgggca ggcacagcca taaccgcacc tgttccataa
     2881 ctagcaagaa catagtccgc aatccagatt ggcatttcct taccattgac agggttgatg
     2941 gcataggcac ctgtccaaac accagttttt tccttagcaa ggtctgtacg agccaagtca
     3001 gacttaaggc tggcttggtg tttatagtct actacagctt ctgcttgctc tgaacttgtg
     3061 atagcgtcta ctaattcatg ttcaggagcc aagacagtga aagtcgcacc gaaaagtgtg
     3121 tccggacgag tagtaaagac tgtaaattcc ttgtctgttc cttttacttt gaaagttaca
     3181 ttggcaccag ttgatttacc aatccagttg cgttgcatat ccttgataga ctctgaccaa
     3241 tctagttcat ctaagtcatt gagcaagcgc tctgcgtaag ccgtgatttt gagcatccat
     3301 tggcgcattg gtttgcggac aactggatag cctccacgct cagaagttcc gtcaggaagc
     3361 acttcttcat tggcaatggc agttcccaat tcctcaaccc agtttactgg cacttcagct
     3421 tcataggcca agcctttttc gtaaagcttg gtgaaaatcc attgagtcca cttgtagtag
     3481 tttggatctg ttgtgttgac ttcacgatcc cagtcataag aaaatccaag cgcattgatt
     3541 tgacgtttga agttggcaat gttttccgat gtaaattctg ctgggtcatt accagtatcc
     3601 atagcgtatt gctctgcagg taaaccaaaa gcatcccaac ccattgggtg aaggacattg
     3661 tagccttgcg cacgtttgta acgactgagg atatcggttg cagtataacc ttctgggtgt
     3721 cctacatgca gaccagctcc agacggataa gggaacatat caagcgcata aaacttaggt
     3781 tttgatgcat ctgttcctgt cttaaatgta tgatgttctg cccagtagcc ctgccactta
     3841 ggctcaattt ctttatgatt gtaaaaactc atggtctctc tccaattttg tgatgttact
     3901 attataccat ttttgaggga atttgaaatg tgtttaaaga aaaaactgta tactgtatct
     3961 cattaatttt gatgactatg aattgctcta tctcccactt tttatcccat tgttatgcag
     4021 taaatcaagg agtaagtcaa gaaacttatc tcgtatagca caaaatactt attttagacc
     4081 gaagataaaa ctggaatgct aattaatgta ttcacaataa atagccgaca acctatgtcg
     4141 ctttttgatt tatgaagaac gtatagtgga ctgaatctga aatagtacga aacaattgct
     4201 aaaacattta tagaaattaa ttttactttc ccaatcgatt tgttctcatc ttatttcaat
     4261 ctgctataga attcaatacg aggatcaata aaagactacc atttggaaaa cagtagtcct
     4321 tattgtttat tttgaattta cataggcatc tacagcgata atagcttgag caacatctga
     4381 aggcgaaatc ttaaacggca tctgatgaat tgtctcaccc tccatagttg cttgcttacc
     4441 aacttttatt aaatcatcat atccaacttg atccaaatgc atttctttta gagttgttgg
     4501 cataccaatt tttttgtaaa actcaatata cttatcaagt tcttctttag gtctattttc
     4561 caataatagt tgtactaaag ttccataagc tactttttca ccatgtgtta aatgatgaat
     4621 gtcgcctgtc aatgcagtaa aaccattatg aattgcatgc gccgcagcta atcctccact
     4681 ttcaaaacct aggccactca ataaagtatt agcttcaaca atattttcta atgctggtgt
     4741 caccacttta gcttcacaag ctgccatagc ctgtaaacca tctgcaaaca gcgtttcttc
     4801 acatttcttc gcaattgcaa ctccagccaa tgtttgctgt tgtcccaaca tagtttttcc
     4861 atttgcctgc ataaccgcac gcgcctcaac ccaagttgct aaaccatctg caataccaga
     4921 cgctaataaa cgcttagggg cttgtgaaat aacttttgta tcaaccaaaa ctaaatctgg
     4981 atttttagaa taaaatagat aatgatcaaa tgcaccttca tctgtataaa taacagataa
     5041 agcagataca ggtgcgtcgg tcgatgcaat tgttggagca ataataacag gcttttcaat
     5101 caaatctgca atagcttttg cgctatcaat cgtctttccc ccaccaagac cgataatact
     5161 atcacaattt tctttctcag ccaaggcaac aactcgattg atttcattgt cagaagcttc
     5221 accattaaat agcgccagaa caatatggaa accataccta tgtagataat cttcaaatcg
     5281 ttttccaaca atatcataaa ccaactgatc gcataataga atagggtaat ttcccaaatc
     5341 caaaattgat ttggcatttt caaacaaggc attttccccc tgaatatatc tagaaggact
     5401 agcaaaaatt ctcatatgta tttcctctct atctaaatgg aacgactatt ttgagtaaca
     5461 aaccaatcgt cagaaaaatc atcaaccgcc ttttggatag atggcatggc gaaagctgat
     5521 tcaaaaacat ctgctcctgc tgtaacagca tgcgcacctg cagctaaagc attatttact
     5581 tgtgctacat ttttaaagga tgcagccaaa atcttactag gagagttctg tctatcaata
     5641 gcaagaacta attgacgaat gacagaattt gaatcaatgt tcagattttc cattctatta
     5701 taatatggag ctaggtaatc cgctcctgct tcgatagcta ataatccctg aataactgta
     5761 taaatagctg ttgcagtgat atggtagccc tcttttttta gcacctttat tgcacgtaat
     5821 ccagctggag taacaggtac tttgataaat atatcatctc ctgcttgtct tcgaatttca
     5881 tgagcatcct ttaagatgcc ttcaaaatct tgagaaatca cctgaacatg aatagagggt
     5941 gtagagccaa tcaattctct tacatctttg attcgttcaa aaaaattaat agaaccctct
     6001 ctttttgcaa tagtgggatt tgaagttacc ccagctagcg gcaaaatttc agaccacttt
     6061 ttaatctcat ctaaatttaa tgtgtcaagc ataaattcca taaaaagaac ctctttattt
     6121 tacaaagtat gctcagtacg tcctataatg tcatcttggg ttgccttaga aagaacattg
     6181 aagaatgcag agtatcctgc aacacgaaca attaagtctc tgtgtttttc aggatgtttc
     6241 tgagcgtcaa tcaacgtctc tctggaaaca acattgtatt gaatatggta cccatgtaaa
     6301 cgattaaaga atgttcgtaa caaagcaatt agttttaatt tatcttcttc tttggctaac
     6361 gtttgaggat ttactttctg atttaagaga accccaccta cgatttcatc tgttggtaat
     6421 tttgaaacag attttaaaac agatgtaggg ccgtgttgat ccatattatg tgatggtgaa
     6481 caaccctctg ctaacggtgt acccgcgttg cgtccatctg gagttgctaa tgttccacgt
     6541 ccctgcccta cgttggctga tatagaagat gttcctgaat aacgaattcc tccaataggc
     6601 cctcttccat aacgtgtatt aggatattta gcaatttcat caacataaat gtcataagca
     6661 gcagtaacca atttgtcagc ataatcatca tcattaccat acttaggtgc atcatgaatc
     6721 aacatttctt gaatgacctt accttcttct ccggcataat ctgtttccag tgcatgccaa
     6781 agttgacttg ggcttatacg ttcttcctca aacaccaatt ttttaattgc agctaacgaa
     6841 tccgacaaat ttgcaattcc aacttgcaat cctgatatat aatcatatac tgctccacct
     6901 tctttaaggt gttttccacg accaatacaa tcatcagtca atgctgaaca tagaatatca
     6961 ggaacttctc gttccaatga taaatcaata gaattttcaa caataacact cattcgtgtc
     7021 aaatatctta gtgttttatc ccaagcattt tctaattcag aaaagttctt catatcctta
     7081 aaatgaccaa agcttggtgc aaaccgttta cccgaagccg gatcaattcc atcattcatc
     7141 gtgataagta gaaccttagg gaagttcata taactcatac ctgtgcaacg atagccccat
     7201 ttccctggaa ctgccgtttc aacacatcca atggcactgt aatcataagc atcatcttcc
     7261 aatactcctt ttgcaataaa agaaggaata ataatctcat cattattaaa tgcaggcata
     7321 ccaaaaccaa gtttcatcac ttcaatacac tcattcatga aacgagcatc taaacctgca
     7381 tggtaacgta cagttagatt aggttgcggt agatgggttt gtgcaactga ttttaatacc
     7441 aaataagata atgggttaac agcatccttc ttatctcgag tctgtccacc aattgtaaca
     7501 ttttgatata aaggacttcc tgctgaagaa aatgtatgtg attgactgcg aaccttatta
     7561 attgtaattg tcttaatcca aagatttgtc agacgttcaa caatgctatc ttctgtttct
     7621 ttaccacttt ctaaatcagc cttcatatat ggatacatat attgatcaaa acggccatat
     7681 gaaagagagt ggccattaga ttcaatttgt aaaatacatt gaataaacca aactgattga
     7741 atagcttctg caaaagtagt tgccggttca tatgggactc tagagcaaat atctgcaatc
     7801 tcaagtaatt ctttcttacg tttaggattt gcattttcgg ctaaactttt agcaagagca
     7861 acaaagcgct ttgcatatac tttaatagca tcgattacga taaatataga gtcgtaaaaa
     7921 tgatatttat caatacttgc tggatctgtt aaatctagag ctgcttttgc tttacgagcc
     7981 cgctcttcaa aacctcttaa gccaaattgc aaaagtttct gatagttaac tgctaggtga
     8041 gcatctccag aattcatctt accttccata ccgaagaatc ctgtttccat ataaacagac
     8101 acttcttcag gtaataaggc accagctcta gcacgtaaat tattattttc ccaaaacgga
     8161 gcaatacttc taagttgttc ttttgtttct tctgtaatat agaaaacatc tccatcacgc
     8221 ttttcaaaaa gatccaactc attgagaaca aattctagcg tatattccgg aaaaatagga
     8281 gcatctttat tggaagaagc ttgatttccc gcaatcatag attcttcttc aatatagata
     8341 gtcatatttt ccaaaatttc tttcagcata tatgcacgtt ttaggacatt aggttgttcc
     8401 ttgtatcgtt cataggcgcg tgttgctaga actgctctct cagcatcaat ataaggtttt
     8461 ttatttaaaa catcttctcg atatttgttc atcctttcag taaggctgcc aaaatattct
     8521 gtcttgattg ttgttcttgc tacttctgta tttaccatcg taagcacctt tctttcgaaa
     8581 atatttttat ttctttcgaa aatataatac cgatttttat agaaattttc aagcttttaa
     8641 ctagaaaaat tttaactctc taatttttag cacaaatgtg tgagtttaac agtaacacac
     8701 aaatacctct aagattgttt gatttcattt tggtaagcta aacgatcctg tatcttaaag
     8761 aatggaaagt aaaccattgt agacatcgcc aatactaata actgagtcac aacaccttgc
     8821 catccaccca ccaaaaatcc tgatagaata gctggtgtac tccaaggcaa tgttacccct
     8881 gagaatggct gcatgaaacc tgttgcaata gctccatata ctatcacagc tgcaagtaca
     8941 ggaacaagaa tgaaaggtac aaacataact ggattcatga caatcggaaa tccaaataca
     9001 actggctcat ttacgttaaa tattgctgga aaagctgcaa cttttcctaa ggcttggtat
     9061 tgttttgatt ttgctacaaa aagcatggca actacaagac caaacgtaat ccctgaacct
     9121 gatagaatta aaaatgaatc taaaaactgt tgagtaacaa tatgtgctcc attttctaat
     9181 gatagattac cagaggctaa catagcttta ttagcatcaa gattagataa aagcagagct
     9241 gtcactactc catttactac cgattgccca tgaacaccaa accaccacaa aaatgatata
     9301 aagaatgcaa ttccaatagc accatacaaa gatccagtta aaccttgcaa cggaacttga
     9361 atagcagaat aaatcatttc tatgaatgtt ccgccattag tcattgactt cgctaaaata
     9421 tatacaatca tagaagataa gaaaattaca aatgctggaa tcattgcttc aaactgtttg
     9481 gcaatagctt gtggaacttg ttctggcatc ttaataacaa tttttctctt tataaagaag
     9541 gtataaatac ttcctactac caaacctata atgatagcac cgataattcc ttggcctcca
     9601 aaccaaactt tactaatagc gtccccaatc gcctcacctt gtttagggat ataagatgat
     9661 cttagcaaaa taaagaatgc agatacagat aaaactccag ctggtaaagc ctctactccg
     9721 ctattcttag cataagaata ggcaattgaa aaacaagaaa ttagacccat aatagcaaaa
     9781 gttcctgaat atacttgcat aaacggctct gtccaattag ctccaaaaac actagcaatg
     9841 ctcttattta atccttcgaa cggcaattgt cccataatca agaataaact accaactact
     9901 gtcaatggca aaattgctaa catcccatct tttagagcta taatgccacg catattcaca
     9961 aacttcatca tcggtgcaat gattttctga acatccatct ttgccataat aaatctcctt
    10021 ttcttaccca ctaatcaaag atagggccaa atctaatact tttttcccat ctaacatacc
    10081 atagtccatc atcggaataa cagctatcgg aacatcacac ttatcacaaa tttcttttga
    10141 tttatctaat gtataagcaa cttgtggacc caatagtgca acatctatat ttggcgcata
    10201 atccgctaat ttagactgag aaaacgcctc tatttctgcc tcaactccac tagattgcgc
    10261 tgcaattttc atattattta caagcatacc agtagaaaaa cctgctgcac aaaacaaacc
    10321 aatcttcacc attatgtttt cctcctctat gttaataaca atgataatac tctagtatta
    10381 ttttttatga agttcttttc tcaaactaat aatttctttg attaaattaa tctccgtcat
    10441 actggtcatg agatgatctt gtgaatgaac aaagagagct gtaatttctg tctttgtacc
    10501 agacgcttcc tgtgccaaaa attttgtttg tagattatga gcttctaata aggctgcatc
    10561 agctaactgt atttcttctt cacaccgttc acatgtacca ttttttatgt aatctaacgc
    10621 tttataaata tgttgtttcg catcaccagc atataaaatt aaacccatga taatttgatc
    10681 tggaacaata atttccataa attcctcctc atttcactat gtaaaaagct tttttcattt
    10741 gaaattttaa aattttcttt tgtaactata gaa
//
LOCUS       DAVKJJ010000062        10718 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000062 DAVKJJ010000000
VERSION     DAVKJJ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10718
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.062"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(80..1504)
                     /locus_tag="VW144_001774"
     CDS             complement(80..1504)
                     /locus_tag="VW144_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9601186.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2049..4328)
                     /gene="pulA"
                     /locus_tag="VW144_001775"
     CDS             complement(2049..4328)
                     /gene="pulA"
                     /locus_tag="VW144_001775"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEU9601187.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLTPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGTYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4440..6398)
                     /gene="ligA"
                     /locus_tag="VW144_001776"
     CDS             complement(4440..6398)
                     /gene="ligA"
                     /locus_tag="VW144_001776"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU9601188.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6491..7669)
                     /locus_tag="VW144_001777"
     CDS             complement(6491..7669)
                     /locus_tag="VW144_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9601189.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7749..8612)
                     /locus_tag="VW144_001778"
     CDS             complement(7749..8612)
                     /locus_tag="VW144_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9601190.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKRQCKEGCKQMQEAIHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8615..10516)
                     /locus_tag="VW144_001779"
     CDS             complement(8615..10516)
                     /locus_tag="VW144_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9601191.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNNLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLVTEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         3212 a         2355 c         1919 g         3232 t
ORIGIN      
        1 agttcgtatt ttttctataa ttattattat tttatagcaa aaaaataaaa ccaggaaaac
       61 aatttcctga ttttacacgt tatttcacat caaaaacaat ggatttgaca tttgtcatcg
      121 cttcaatgct atatttaatt ccttgcactc cagctccaga acctttgaca ccaaggaatg
      181 ggaaattatc tggaccacgc tgggttttat tattaatgtg gactgtacct acttcaagtt
      241 tttcagcaat ttcaaaggct tttttgaaat catttgtaaa gactgatgat tgaaggccga
      301 attcagattc gttggcaaag gcaatagctt cctctacact agccacacga atgattggta
      361 aaacaggacc aaatggctct tcccatgcca ctttcatatc ttttgtaact tggtcaaaaa
      421 gcactggcca gaggagattg ccctcacgtt tgattggtgt aagagcctga gcctcttttt
      481 cttgtgcatc ctcaatcaag ccccaaatga agtcggctga agcattgtca ataacaggtg
      541 taatatcagc attgtcaaat ggatcaccaa ctgttaattt agaaacttct tcctgaagca
      601 aagtagctaa tttatctgct acactttcga gaacaatgac acgtttaatg gccgtgcaac
      661 gttgtcctga gtagctaaag gctcccgcaa caatttgctt ggcagcatgt tccaaatctg
      721 catcttctag tacaagagct gcatctttcc caccaagttc caacatgata ggacgcatac
      781 cagctaaacg accaatacgt tctccaatag gggttgaacc tgtaaagttg atgaagttga
      841 cttctttgtg ctcaatgata taatccccaa tttctgaacc acgacctgta atggtgttga
      901 aaacacctgc cggaatccct gcttcttcaa atgctttagc caacaagagt ccagaaatgg
      961 aaccttgtgt tggtggctta aacatgacca cattccctgc aatcaaggca ggtgcaattt
     1021 tagaagcaga taaattaact ggataattaa agggagcaat cgctagcacg ataccaactg
     1081 gttcacgacg gacaacagcc agtttgtttt tacttgctgc ctcaaaacca ccaccttcca
     1141 ttgcttgtcc agtgatacgg agaccttcct cagcagcata acgaatcaag tctgctgtac
     1201 gcactacttc tccaattgct gctttaatcc cttttgctac ttctttggca aggatagtac
     1261 caattttttc cttatcgcgt tctaaaatag ctgctgtttt atgcaaataa gccgcacgtt
     1321 caattgctga taaagctcgc catgctggca gggctgcacg cgcagcttgc atagcctcat
     1381 cagcttcagt ctgagtcatg gctggaactg tacccaattc ttcttgattg attggtgaat
     1441 aaatcgtaat ttcttgttca gatgatttcc attttccatt tactaaattc tgatatcttg
     1501 tcaaattctt gtcctcctga aaatgattaa gatatatttt accaaatttt cagaaaatca
     1561 caaccctttt tgcaaaattt ttgagattat tttcacaagc ttgatttttc aaagtatact
     1621 caataaaaat aaaaaaaatc cactacatca aggcgagact aatgtggttt gaagaaattt
     1681 tcgaagagcg tgaatgagta tcatctatag taaaataaaa aaactgaaca atttggttgg
     1741 ggacagtcaa accaatttct cacaatgttt tagaaacaag ggtgtgctat tccaatttca
     1801 ggctactata actgtcatag attgctgaaa caaagtctag gtaaaagtct tcataataaa
     1861 aagaccccct atcaagtgtt caaaaacttt gataggaggt cttgttttgt gaaaatattt
     1921 atcaaatttt ctatacaagt gagctgttag ccaggttctt tctattcttt caatttcaat
     1981 gaatggattt tttaccaata ctcataactg ggaatttgtc tgtgtaaaaa tagcgagata
     2041 gatggtattt ataaaacact caagacagct agactaatat catttaaaac attatcttct
     2101 tttgagcgac tgttggttac caacatagct aaatttcctg cattttcaaa ttgatagggt
     2161 tctgatttag cattcacaac taccaagagg tgttctttgc cgtgaacttc atagataagg
     2221 cagccgctat gttcaatcgc agaatgcaca aagacatgat ggtaaatttc atcatagcta
     2281 gagtaagaaa aggcaccagt ttttgtcttc aatcggatga cttgacggat aaactcaata
     2341 ctgtcttgac gctcattaat caagttccag ttcacttggt tcacactgtc aggagcatta
     2401 tagctattca tcgcacgctc tctatcatca tgggtcaact caccattttc accagtcgca
     2461 accagtttgg tacgaccaaa ttcttgaccg atttccataa aggccatccc ctgcatgagc
     2521 agattcatgg ctgtggcagt ttcgaccttg cgcatgattt gctctgaact ttggtctgga
     2581 tgaagggttg ccaataaatc gtgaagattg taattgtcat gggcttctac atagttaagc
     2641 acctgatttg gatgtgtata ggttcctaat tcacgacttc ctaggattgc tttagctaga
     2701 attggctctg tcgcagcacc actgacaaaa cctgacttga tagcaccata aacttctccc
     2761 cctttgacag catcgcgctg attgtcatta aagaaaccaa tatttggcat ctggtaggca
     2821 ttgtccttct tggccttatc ataaggggta agacctgttc ccatatccca tccttctcca
     2881 tagaggataa tgttggagtc gatttcatcc aagctttgac gaatcatctg catggtcttg
     2941 acatcatgaa tccccatcaa gtcaaaacgg aagccgtcaa tattatattc ctgcacccag
     3001 tatagaagag aatcaatcat atacttgcga aacatttcgt gttcactggc tgtttcattt
     3061 ccaacacccg ttccattctg gaaggtacca tctggattca tacgataata gtaatcaggg
     3121 actgttgttt ggaatggtgc atcaacaact gagaaggtat ggttatagac tacatccata
     3181 atgactccaa tacccgcatc gtgataagct tgaaccatca ccttcaaatc acgaatgacc
     3241 tgagctggat catctggatt agttgaaaaa ctagtttctg gcgcgttata gttttgtgga
     3301 tcataacccc agttgtaggt tacatttcca tcctcatcgt attctttatg acggtctgca
     3361 attggttgca attgaacata attgtagccc agcttcttga tgtaatcaaa agcagttgac
     3421 tggccgtatt ggttaactgt tccagcctga gcagcaccca agaaagttcc tcgaagatgt
     3481 tcatctacac ccgatgtagg tgatttagtc aaatcacgaa tgtgcatttc acagataact
     3541 gccttacatg gattttccaa gcgccaagta gcctccgaac cgtgcttaac ctcgaagttt
     3601 tcaacttgct tttctacatg gctcagaata gctgaacgtt tgccatcagg gctggtcgcg
     3661 attgtataag gatcacgtgt cagtgtttgg tgatgaggga attggacttg atactgataa
     3721 gtcttaccta ccaaatcttc ttcaacatcc aaactccaga caccgattgt attgtcctta
     3781 tgattataag agtagctatt gcctcttttc atctcaaaag tcttccaaac gggtgcatca
     3841 ttagcagctg attcataaac gacaacttgc acttctgtcg ctgtaggtga ccagagggaa
     3901 aaatgagcct gattgtcctc tacacggcaa cccaattctc cttggtaacc ccaatgatga
     3961 tcaaaactag cactgttaat ggccttatca aaggcaaaag gattttgatt tttatagaaa
     4021 ggactggcaa tagcaggatt ttcagagtaa taaatcctat catcgccttc caaaatccag
     4081 acctctgtca ataggggata gtgattaaaa cggatagaat attctttact agtttgacct
     4141 gtatgaacca caaaattcaa gctttctata acatgtgaac ttgggtgttc aaagctaaat
     4201 aaagctccaa aataatcttc tttgtaggtt agcaaatcaa ttcgttgatc ctgacttttt
     4261 acaaaggagc aagtgtcata ttctccattc ttacgatggt aatgaatgcg catagggtag
     4321 ttatacattt tttatttttc ctttttactt tgtttctatt tcactaataa atttttgtca
     4381 atctcgtctc aattaacaga catagtcata ttctctaaac tctgttttta aacgatccat
     4441 tacaaacttt ctagccatgc ctcatctctg acctggatac caagttcttg tgctttttgc
     4501 agtttacttc cagcgtctgc acctaccacg acgaggtcgg tctttttaga aacactacct
     4561 gtcactttgg cacccagact ttcgagttta cttttagctt ctgagcgctt gagtcgttcc
     4621 aattttcctg tcaataccac ggtcaaacct gacaaggccg catccgctac taccgtctgt
     4681 cctttatagt ccagattgac cccagtttct ttcaattctc tgagcagaat ttcagagcct
     4741 tctgtcgcaa aataagtctg aagacttttg gcaatcacgc cacctagact ttcaatacta
     4801 gccacttcct ctgaatctgc ctgatacaga ttttcaattg aatggaaata ttgaagtaaa
     4861 agctgactag ccttgcttcc gacatgacga attcccaaac caaataagag cttctcggca
     4921 gaattttcct ttgatgcttg gatagcctga tacagtttag cagcggactt ttccttaacc
     4981 ccctctaaaa ggaggaaatc ctcttcttgc aaacgataaa tatccgccac atccttgact
     5041 aaattagcag caaaaagctt ctcaacaata gatggaccaa ggcctgtaat attcatagca
     5101 tcacgagaag caaagtgaat caagccttcc atgatttgag cagggcaacg cggattgata
     5161 caacgtaggg ccacttcatc ttcaaagtgc aacaagtcag agttacaact tggacagttt
     5221 gtagggatat ctagtttttc ttcagaaacc cgtttggact ctaccacacg taaaacggca
     5281 gggatgatgt caccagcctt atatacaatg accgtatcgt cttttcggat atctttttca
     5341 gcaatataat ctacattgtg cagggtcgca cggctaacag tcgtaccggc aagttgtact
     5401 ggtgttagat tagcagttgg agttacaaca ccggtacggc caactgtcca gtcaactgat
     5461 aagagttgag cttctttttc ttcagcaggg aacttgtagg ctactgccca ctttggagcc
     5521 ttaacggtaa aaccaagttc ttcttgactt gctaggtcgt tgaccttgat taccactcca
     5581 tcaatatcgt aaggcagatt ttcccgttcc tgtcctactt cttggataaa attccagatt
     5641 tcatctatgt tttcagccaa gattcgctta ggattgacca caaaacctag ttgttctagg
     5701 tacttcaaac ccttttcttg gctatcacga gttgaagggc tggcttcttg atagagaaac
     5761 gttgcaagat tacgcttggc aactactgct gtatccaact gacgcagagt tcctgctgcc
     5821 gcattacgag gattagcaaa ttcaggctct ccattttctt ggcgcacttg gttaacttgg
     5881 tcaaaggaag cgcgtggcat gtaacattcc ccacgaactg tgatatctag ttcttctggc
     5941 aaagtcaaag ggatgtcctt aacacgcttg aggttttctg tgatattttc accaattgaa
     6001 ccatctccac gtgttacccc agcaaccaaa atcccctttt cataagtcag cgagatagat
     6061 aagccatcga ttttcagctc acaaatatag gtcggatgag ccacttcctt acgaacacgc
     6121 gcatcaaaag catctagctc ctcacgtgaa aaagcatcct gcaaactata aagaggatac
     6181 tgatgactgt atttttcaaa accatctaaa accttgccac caacacgatg agtcggactg
     6241 tctgctagca cttgctctgg ataagcagtt tctaactcga ccaactcacg gtaaaggcgg
     6301 tcatactcac tgtctgaaac cgagggatta tcgctggtat agtactcagt cgcatagcga
     6361 ttgagcaaag cgactaactc attcattctt ttattcataa gaccatttta ccataaaaca
     6421 agccctcctc acaaacgaga aggacggaaa aaacacttag tttgaaatta tttttgaaac
     6481 tcaagcaacc ttatatcaat ttttcaaaat gagttcgaac ataaataaac gatatacaag
     6541 acaagatgat aacaccactt ccaattatca ggaaagaaga gagatgtaca cttggcaaga
     6601 ctgtcataaa tccttttgca ataggcataa atagaatagc taaggtaaaa attgtactca
     6661 gtactctccc aagaaattcg ctctcaacct tggtttgtac ttgagtaaaa aagtgaatat
     6721 taaaaatcgt cataaacaat tcacaaacta aatttccaga aaaggaaaga aaagttggaa
     6781 gtggtaatcc catcataaaa actccgacac ctgtcaaagc cagtaaaatc aaaagattat
     6841 aaatattagc tttaatttta ctagctagaa gagccccaat gatggaacca atagccccca
     6901 tagttaaaat acttgcatag gctccttctg atccgtaaag ctgattcgaa aagggaagta
     6961 gaaattcaaa agctgcaaaa aagaaattaa cgctggaagc taccagcaaa aggaagaaaa
     7021 tttcttgctg atgccagata tagtgtaacc catccttgat atctacaaaa atatctctcc
     7081 cagtaaaagc ctttttctct tgaacttttg cttcctcttt tggaaggaaa gccactagaa
     7141 caaaagcaat gaaaaaagtc agcgagtcta gcagtagcgt catatggaga cttgcaaact
     7201 gtaaaacaag gaaggaaaga acaggagagc taacacctac aacctgcaaa accagctcta
     7261 ggcgagaatt atagatcaca atctcatctt tctccaccac ttcagttatg atagctttat
     7321 tggctgtgcg agaaaaggca aaagcaatag cctgcacaat gttagcaaca atcaaagcgc
     7381 caatcatcca gctatcattc cttatgaaag aaatagccag acaaagaatc ccacaaacaa
     7441 gatctgccgt cattaaaatc ttacgacgag aaaaacggtc tgaaataact ccgccaaagg
     7501 gattgacgag aatagatgtg acgagctcag aaatctgata cattcctaaa actgtctgtc
     7561 ctatagtccc catagaagcc aaccagacac tatttccata atcatagagc atatttccca
     7621 ttttattgat agccccacgg ctaatcaact gcactgcata gcgattcata ttaaagctcc
     7681 tctcaaattt tgaaactatt gtatcaaaac cgaaaggagc tttttatttt ttcccttatt
     7741 tgggaaaatt aacttttgac aaatttttcg tagtgttcct gataataggc tacttgctct
     7801 ggaagaccta acacatcaaa aatatgtatg gcctcttgca tctgcttaca gccttcttta
     7861 cactgtcttt tttgatataa ggcaaaacct tttaaataat ggaaaacatt acgctcataa
     7921 agcttaatac ctttgtcaat aatcttctct gtataagcct caaaatagtt ggcattataa
     7981 aaagaagaat gctctaaaca atgctggtaa caattgaggg ccaaaatcaa aactaatctc
     8041 ttatggcgac taatctcttg gtaaaattcc tccctctcca taacttctct accaatccga
     8101 gtgacatagt ctacatcgta gaaactatag aggttaccga aaagaatcaa ctcatacatg
     8161 gtccattctt ctgttttgaa gagataatct gctaccttat ccaaatcatc ctgcttcata
     8221 tcataactcg catctctttg acaaatcaga ccttgtagca aaatccagtt cagctcaaaa
     8281 taaaggggag tcgtcgaact cttagacttt tcaagttgtt ctctttgaag cttttgaaaa
     8341 cctgcaatat cgtttgaata gtaaagtggg ataatctgtg ccatcataga cacatgttca
     8401 tgattgtgaa aattccttgc cttatccatg aaattttcga ttgttacatg aatgttatcc
     8461 aaaatctcaa agaaacggga gactgccagg tcagactccc caagctcaaa gcgagataac
     8521 tgagaggtag agcaggattc gcctgctgct tcctttaaag aataatttcc acttgttcga
     8581 aattcacgaa atacttttcc aagatgttcc atctttacac ctgatctgat aattcttccc
     8641 actcaagcat agcttcttcc tgacgatggc tgattttgtc cagctcagcc tgtaattcca
     8701 tgagtttgtc ggcatcgttt gtttccaaca tttgttcaga aatggcttgg ctttgacttt
     8761 ctagctcttc aatttcagct tctagacttt cgatttgtcg catgagtttg cgaacttctt
     8821 tttgactttc tttctgggcc tgatagtcat tgactggact tgcttccttt gcttgattgc
     8881 tagttgaagc ttcctcagtc tgactcattt ctgctgttgc tttcttctca acatagtagt
     8941 cgtaatctcc aaggtagaga gttgaaccat tctcagacaa ttccaaaaca tgagttgcca
     9001 cacgattgat aaagtaacga tcatgactga caaaaagcaa ggtaccatca aagtcaatca
     9061 aagcattttc caacacttcc ttgctatcaa tatccaagtg gttggtcggc tcgtcaagga
     9121 tcaggaagtt gttgttttcc atagacaatt tagctaaaag caaacgagct ttttcgccac
     9181 cagatagcat gccgactgat tttttaacat catctcctga gaaaaggaag gctccaagac
     9241 ggttgcggat ttcaacttct ggtgtcagtt tgaaatcatt ccagagttca tccagcaccg
     9301 tattacttgg tgtcagcttg ctttgggttt ggtcatagta accaacctca acattagcgc
     9361 caaagcgctt ttcacctttg ataaaaggaa tctggtccac aatagacttg ataaaggttg
     9421 acttgccgat accatttgga ccaacgatag cgacagcatt catcttacga agatctaggt
     9481 tgataggttg tgacaagact tccccgtcat aaccaatagc agtattttca acagtcaaaa
     9541 caacattgcc cgacgttttt tcagactgga aggtcatgtt ggctgctttc ttgccagctt
     9601 caggcttgtc caaacgctcc attttttcta gttgtttacg gcgagattga gcacgtttag
     9661 tcgttgaagc acgaactaga ttgcgattga caaagtcttc cagagcagcg atttccttct
     9721 gttgcttttc atagtttttt gcctcagtaa ctagcttttg ctccttcaat tcgacaaaac
     9781 gagagtaatt ccccacatag cgatccaagg aatgcttggt caaatctagc gtaattgtcg
     9841 caaccttgtc caagaaataa cggtcgtggc tgacgataat gagggcaccg ctatagttta
     9901 ccaagtaatt ctctagccag gcgatggttt caatatccaa gtggttagtt ggctcgtcca
     9961 agaccaagag attgggcttt tcaaggagca ttttggcaag tgccaaacga gtattttgac
    10021 caccagaaag ctcagcaatt ttcatctgcc acatagactc gtcaaacttg aatccattca
    10081 aaatcgctcg aatatcagct tcataggtaa agccacctgc ttggcgaaaa ttctcagata
    10141 agcggtcata atctgacatc agtttatcca aatcctcacc agacttttca cccatctcca
    10201 gctccatctg acgcagttgt ctctccgtcc gacgcaaatt attaaagaca tgaagcattt
    10261 catcgtagat ggtattttca gactcaaaac ggctatcttg ggctaggtaa gacagagaaa
    10321 tatctttttt cttattgatt tctccgctag ttggctcctc ttctccaact aaaatcttca
    10381 aaagagtaga cttacctgca ccatttttcc caacaagggc aatccgatct cgttcatcaa
    10441 cctgcaggtt gatattatcg aaaagaacct ctcctgcaaa agaacgttca attttattag
    10501 cttgtaaaat aatcataaga gtagtatagc atgtttccct aaggcattca agataatcgt
    10561 aagtctttta gtacaacttt tataacataa aataaactaa attatgtata ttttatatta
    10621 gattcttcac tatcttgttg gattttctaa ccagctaatc ttgtttcaaa tagttatcgc
    10681 acaagcctat tatttaattc ttttcattat ttacgtac
//
LOCUS       DAVKJJ010000063        10622 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000063 DAVKJJ010000000
VERSION     DAVKJJ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10622
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.063"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            241..1119
                     /gene="truB"
                     /locus_tag="VW144_001780"
     CDS             241..1119
                     /gene="truB"
                     /locus_tag="VW144_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU9601192.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVIAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            1360..1662
                     /locus_tag="VW144_001781"
     CDS             1360..1662
                     /locus_tag="VW144_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9601193.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            1770..2408
                     /gene="udk"
                     /locus_tag="VW144_001782"
     CDS             1770..2408
                     /gene="udk"
                     /locus_tag="VW144_001782"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEU9601194.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            2536..3876
                     /locus_tag="VW144_001783"
     CDS             2536..3876
                     /locus_tag="VW144_001783"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU9601195.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMVTAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            3854..4066
                     /locus_tag="VW144_001784"
     CDS             3854..4066
                     /locus_tag="VW144_001784"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU9601196.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIDLEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            4063..4938
                     /locus_tag="VW144_001785"
     CDS             4063..4938
                     /locus_tag="VW144_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU9601197.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDSYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            4931..5746
                     /locus_tag="VW144_001786"
     CDS             4931..5746
                     /locus_tag="VW144_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU9601198.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     NLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKVHQNVLESVTAMAVEV
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            5739..6170
                     /locus_tag="VW144_001787"
     CDS             5739..6170
                     /locus_tag="VW144_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9601199.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            6177..7844
                     /gene="recN"
                     /locus_tag="VW144_001788"
     CDS             6177..7844
                     /gene="recN"
                     /locus_tag="VW144_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU9601200.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMTEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQYGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            7846..8574
                     /locus_tag="VW144_001789"
     CDS             7846..8574
                     /locus_tag="VW144_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEU9601201.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            8653..10476
                     /gene="lepA"
                     /locus_tag="VW144_001790"
     CDS             8653..10476
                     /gene="lepA"
                     /locus_tag="VW144_001790"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEU9601202.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMELERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVTEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
BASE COUNT         3139 a         1917 c         2591 g         2975 t
ORIGIN      
        1 ttttttattt agatggcatc aaaagtatac tcccattatt atctttagat gacagacgaa
       61 atgtattaca acatattcaa aaacagaaca attgtcctcc tccagagatt gaggaattac
      121 aaagaattat agttaattaa attttgtaaa aaagagaata aaaggtagac gacgcctcag
      181 taaaaattgg cccggaaaaa agcgaagttc gaagcactga agggggagta gaaagcaaaa
      241 atgaacggta ttatcaactt aaaaaaagaa gcaggaatga cctcgcatga tgcggttttt
      301 aaactgcgta agattttggg aaccaagaaa attggtcatg gtggaacctt ggatccggat
      361 gtggtgggtg ttttgccgat tgcggttggc aaggcgacac gcatggtcga gtttatgcag
      421 gacgagggta agatctatga gggggaaatt actctgggtt attccacgac gactgaggat
      481 gctagtgggg aagtgatcgc agaaacccct gttttgtctc ccttggatga aaagcttgtt
      541 gatgaagcga ttgctagctt gactgggcct attacccaga ttccccctat gtattcggca
      601 gttaaggtta atggtcgcaa gctctatgag tatgcgcgtg ctggtcagga agtggagcgt
      661 ccagaacgtc aggtgaccat ttatcaattt gagcgaacaa gtccgatttc ttatgatggc
      721 caacttgctc gattcacttt tcgtgtaaaa tgcagtaaag ggacttacat ccgtactttg
      781 tcagttgatt tgggtgaaaa gcttggttat gcggctcata tgtcccattt gactcgtact
      841 agtgctgctg gcttacaatt agaagacgct cttgccttgg aggaaattgc tgaaaaagta
      901 gaggctgggc aattagattt tctccatcct ttagagattg ggacaggtga ccttgtcaaa
      961 gttttcctaa ctccagaaga ggctacagaa gttcgctttg gtcgttttat tgagctagac
     1021 caaacggaca aagaactggc tgcctttgaa gatgataaat tgttagccat tctagaaaaa
     1081 cggggcaatc tctataagcc aaggaaggtt tttagctaga tcgtttagga ataaaaatcg
     1141 ggtgatagat aacaattgct tgataaaacc ccatactaat agtagaatgg ttttgggaat
     1201 tataatattc caattgttgc gagttgtagg tactcaaata atctatatag aaatttagag
     1261 gtgtgaaatg aagcaattta aaattctttc agataaatat ttagagtcca ttacaggttc
     1321 tggtgggaac ttaggcccag gatttggtgt gataattcca tgatgcgaaa tgagtttcga
     1381 gaaagggtgg agcaacttct tcaacaaaaa gaaataaatg aaaatagtga gttgagtcac
     1441 ctgtttcgtc ttgctataca aaatttagac agaaatgaaa aataccaatc ggtcatggcc
     1501 aatttgagtc aagggttgtc actttacctc atgacgcatc attaccaggc acctaagtct
     1561 gtcattgatt ttggtttatg gattgccaaa gctcctagcc aggaaagagg gagactggct
     1621 ttcttgcaga tgcttgctca aaccctacag ggctttcgtt aaaactgtcg aacatcagag
     1681 ggaagactct ggtgtttttt gctgatgaga ctataaattt tgtaaaaaat gtgatagaat
     1741 agacgacgga taaaataacg gaggatagca tgcaaaatag accaatcatt attggagtga
     1801 caggtggttc tggtggtggt aaaaccagtg tttcaagagc cattttatcg cattttcctg
     1861 atgaaaagat ttccatgatt gagcatgatt catactacaa ggatcagtct catttgacct
     1921 ttgaagagcg tgtcaaaacc aactatgacc atccttttgc ctttgataca gacttgatga
     1981 tcgagcagat taaggaattg ttggcagggc gtccggtgga catcccgacc tacgactata
     2041 cagagcatac acggagtagc aagacctatc gtcaggagcc tcaagatgtc tttatcgttg
     2101 agggcatttt ggtcttggag gacaagcgtc tgcgcgattt gatggatatc aagatttttg
     2161 tggatacgga tgacgatgta cgtattattc gtcgtatcaa gcgtgatatg gaggagcgtg
     2221 gccgtagcct tgatagcgtt attaaccagt acttaggtgt ggtcaaacca atgtaccacc
     2281 agtttatcga gtcaactaag cgttatgctg atatcgtcat tcctgaaggg gttagcaata
     2341 ccgtggctat cgacctgttg acgaccaaga ttgcaaagat tttggaagaa gctcgaaaca
     2401 gcaaataatc acatgtggag gccttgcctc ctttttctat ttttccctca taatggtaca
     2461 taaatggaga tttttaggca tatttttggt ataataatac ccatgaaaga gcaagtaaat
     2521 gaatagtagg tggagatgga aaagtattta tcggtaacaa ctttgaccaa gtatctgaaa
     2581 atgaaattcg ataaagaccc atacttggaa cgggtctatt taactggtca agtttccaac
     2641 tttcgtaaac gacctactca ccaatatttc tccctaaagg atgaccatgc agttattcaa
     2701 gcgaccatct ggtctgggat ttatcagaaa ttagggtttg acctggaaga aggaatgaag
     2761 atcaatgtga ttgggcgtgt acaggtctat gaaccaagtg gtagctactc catcatcatt
     2821 gaaaaagctg agcctgatgg ggttggggcg cttgcgattc agtttgaaca acttaagaaa
     2881 aaattgacag aagaaggcct gtttcaagaa cgcttcaagc aagctctgcc ccaattttct
     2941 aagagaattg gtgtagtaac cagccgtagt ggagccgtta ttcgagatat tatcacgacc
     3001 gtcagcaggc gattcccagg tgttgacatc cttctttatc cgaccaaggt tcaaggtgaa
     3061 ggggctgcgg aggaaattgc tagaaatatt gcgcgtgcta atcaacggga cgatttggat
     3121 ttgctcatta ttggtcgtgg aggtggttct attgaggatc tctgggcctt taacgaagaa
     3181 attgtggtac gagctatttt tgaatctcgt ttgccagtta tctctagtgt ggggcatgag
     3241 acggatgtga ccttggcaga ttttgtggca gatcgacgcg ctgcaacgcc aacagcggcg
     3301 gctgaactgg caacacctgt gaccaagttg gatgtattag ctcatttgca aaatcaggaa
     3361 aaacggatgg taacggcagt ccgaaatgtt ctatctaaga aacaagaggc tttgaaaaaa
     3421 tgcagtcagt ctgttatctt tagacaacct gagcgcttgt atgacggtta tttgcaacgc
     3481 ttggaccaac tgcaactgcg tttgaaacaa agtttgcgaa ctcggatttc tgataacaaa
     3541 caattagttc aagcaagaac tcatcaatta gtacaattat cacctgttac caaaatccaa
     3601 cgctatcaag accgtttagg acagttggac aagctcttag gtagccaaat ggcgttagtt
     3661 tatgacgcca aggttgctga ggccaagcga ctttcggaag ctttgctcat gttggatact
     3721 agccgaatcg tggcgcgtgg ttatgctatt gtcaaaaaag aagagtccgt tgtagattcg
     3781 gttgagagtt tgaagaaaaa agaccaagta acgcttttga tgcgagatgg tcaagtagaa
     3841 ttagaggtta aagatgtcaa aacaaaagaa atttgaggaa aatctagcag aactggaaac
     3901 cattgtccaa agtttggaaa atggtgaaat tgatctggaa gatgcgatta ctgcctttca
     3961 aaagggcatg gtcttgtcaa aagagctcca agctacgctg gacaaggctg aaaagacctt
     4021 ggtcaaggtc atgcaagaag acggaacaga aagtgatttt gaatgaaaaa gcaagaaaaa
     4081 ttagctcttg tcgagtcggc tttggaagat ttttatggag accagcagtt tgcctctagt
     4141 ttacgggagt ctgttctcta ttctattcat gctggtggca agcgtattcg gccttttctc
     4201 ttgttagaag ttctggaagc cttgcaggtt accatcaaac ctgctcacgc gcaggtagct
     4261 actgccttgg agatgattca tacagggagc ttgattcacg atgaccttcc tgctatggat
     4321 gatgacgatt atcgaagagg gcggttaacc aatcacaaga aattcggtga agctatggcc
     4381 attttggctg gagatgcctt attcttagac tcatatgcct tgattgcgca ggcagatttg
     4441 ccaagtcaga ttaaggtgga cttgattgcc aacttatccc ttgcttcagg tagtctgggt
     4501 atggtggcag ggcaagtttt ggatatggag ggcgaacacc agcacttgtc tctggaagaa
     4561 cttcagacta ttcatgccaa taagactggg aagttactag cctatccctt ccaagcggca
     4621 gctattatag ctgaattgtc acctgaaatg caggtgaagc tgaaaactgt gggtgaattg
     4681 attggacttg cttttcaagt cagagatgat gtactggatg tgacagctag ttttgaggaa
     4741 atcggcaaga cacctcaaaa ggatctgcag gcagaaaaat caacctatcc tgccctgttg
     4801 ggcttggaag agtccattgc cttttgtaac caaaccctgg atcaagctaa tgaaaaatta
     4861 gaagaaattg cccagcagct tccctttgaa acagaatcga ttgtaagtgt agtagaaagt
     4921 ttgagaatca atggctaagg aaagagtgga tgtactagct tataaacagg ggttgtttga
     4981 aacgagagag caggccaagc gaggtgtgat ggctggccta gtcgtagcag tccttaatgg
     5041 agaacggttt gacaagccag gagagaaaat tccagatgac accgaattaa aactcaaggg
     5101 ggagaaactc aagtatgtca gccgtggtgg tttgaaactg gaaaaggcct tgcaggtctt
     5161 tgatttgtcg gtggatggcg cgactacgat tgatatcggg gcctctactg gaggttttac
     5221 cgatgtcatg ctacagaata gtgccaagtt ggtctttgca gtcgatgttg gtaccaatca
     5281 gttggcttgg aaattacgcc aagacccacg agttgtcagc atggagcagt tcaatttccg
     5341 ctatgctgaa aagactgatt tcgagcagga gccgagcttt gccagtattg atgtgagttt
     5401 catttccctt aatctgattt tgccagcctt gcaccgtgtc ttggctgatc aaggtcaggt
     5461 ggtagcactt gtcaaacctc agtttgaggc aggacgtgag cagattggga aaaatggaat
     5521 tattcgagat gctaaggttc atcagaatgt ccttgaatct gtaacagcta tggcagtaga
     5581 ggtaggtttt tcagtccttg gcttggactt ttctcccatc caaggtggac atggaaatat
     5641 tgaattttta gcgtatttga aaaaagaaaa gtcagcaagc aatcagattc ttgctgagat
     5701 taaagaagca gtagagaggg cgcatagtca attcaaaaat gaataaaaaa gaaagacttg
     5761 aaaaaattag aagattggtg acagattatc aaatcggcac gcaagaagaa attgtagaac
     5821 atttgaaaga agcaggtatc actgccactc aggcgacggt atcccgagat atcaaagagt
     5881 taggtattgt caaaattcct ttgagagaca acacttatgt ctacgaatta ccaaaatcaa
     5941 tcgtaaaaag tctgcaactg gctgaagaca atatcgaatc ggctgaattg atggataaga
     6001 tgatcaatct ccaagttatt ccaggaaata cggcttttgt aaaagctcag ttaatcgaga
     6061 cttttgcaga caagattttt agctgtttga ctgatgatag ctcgatttta gtcattgcca
     6121 gaagtggaag tctagcagag gaaatctttg aacaagtaaa aaattggtag gtctatatgt
     6181 tacttgaaat ttcgataaaa aactttgcca ttattgaggc tatttccctc aattttgaaa
     6241 aggggatgac tgtcctgact ggtgaaacgg gtgcagggaa gtcgattatc attgatgcca
     6301 tgaatatgat gttgggagct cgtgcgacga cagatgttat tcgtcatggt gcaccaaagg
     6361 cagagattga ggggcttttc tcagttgaga atagtcgtct tttacaggaa atttttgatg
     6421 agcaaggttt agaattaggt gatgaaatta tcatccgtcg ggaaatcttg caaaatggtc
     6481 gtagtatcag ccgtgtaaat ggtcagatgg ttaatctgtc tgttttgcga gctattgggc
     6541 aacatcttgt agatattcat ggtcagcatg accatgagga gttaatgcgt ccccaactgc
     6601 atatccagat gttggatgaa tttggtgatg ccgctttttg ggacttgaaa gaaacctatc
     6661 aaacgagttt tgatgcctat cggaaaatgc gtaagcaggt tctggaagtc aagaaaaacc
     6721 aacaggaaca caaggcacgt atcgaaatgt tggaatttca aatgacagag attgaggcag
     6781 caaacttgca ggctggagaa gacttggctc tcaatcaaga acgagataaa ctcctcaacc
     6841 acaaaaatat tgcggataca ctgaccaatg cctacagtat gttggacaat gaagattttt
     6901 caagtctggc caatgttcgt tcagctatga atgacatgga aagtgtcgaa gagtatgatc
     6961 ctgaataccg tgaaatttca agctctctgt ctgagaccta ctatgtttta gaagacatta
     7021 gcaaacgttt ggaagctatc attgaggacc ttgattttga tggcaatcgc ctcatgcagg
     7081 ttgagaatcg tttggacctc cttcatacta ttactcgtaa gtatggtggg actgttgatg
     7141 atgttttgct ttattttgcc aagattacgg aagaatacaa tctcttgaca ggcaataatc
     7201 tttcgtctga ggacatggaa gcagagctta agaagttgga agtcaatctt gtcaatttgg
     7261 caggtcaact tgcttctgct cgtcataatt tggctcagca actcgaagct gagattaaac
     7321 aagaactgca agatctttat atggaaaaag cccagtttca ggttcgtttt agtaagggaa
     7381 aattcagtcg tgagggaaat gaaatggttg agttttatat ttcaaccaac cctggagaag
     7441 actttaaacc cttggttaag gttgcttctg gaggggaatt atctcgtctc atgttagcca
     7501 ttaagtctgc cttttcacgt aaagaaggca agactagcat tgtctttgat gaggtggata
     7561 cgggagtttc aggtcgtgtt gctcaagcta ttgctcagaa aattcataaa attggtcagt
     7621 atggtcaggt tttggctatc tcccatttgc cacaagtaat tgcgattgca gattatcaat
     7681 tctttattga gaagattagt aatgaccatt caacggtttc gactgttcgt ctcttgacag
     7741 tcgaagagcg agtggaggaa gttgccaaga tgttggcagg tgatgatgtg acagaagcag
     7801 ccctgacgca agccagagaa ttgttgagaa acagggagaa ataagatgac agattattat
     7861 gtaattggag atgttcacgg aaaagctggg atgctggaag accttcttaa aacctgggat
     7921 ggtcagaccc agttgctctt tctaggtgat ttgattgaca gaggtgagga tagtcaccgt
     7981 gttcttgaaa tggtcaagga cttggtggac aatcaagggg ctatctgttt gtctggaaac
     8041 cacgagtata tgtttttgac ttggctagat gacccagaag aaagttatga ccattatcgt
     8101 cgcaatggtg gagatacaac cattaactct atcctaggtc gtcccttgga tgcaccagtt
     8161 gatggagtag aagatgccaa gcgggttgcc gctgaagcgg cagacttggt cgaattcatt
     8221 cgtcaaatgc catttgtagt agagacagac aagtatatct ttgttcacgc aggtattgat
     8281 ttgaccttgg acgactggca tgaaacaaca gattataaaa aagtatggct tagaaaacca
     8341 ttccacggag ctgaaaatca tactggaaaa accattgtct tcggtcatac accggtctat
     8401 ggtttgttac agcaagaacg cggtacatct gagctttgga ctacagaaga tggcaagatt
     8461 ggaatggatg gaggagctgt ttatggtggt gtccttcacg ggattgtctt tacagaccaa
     8521 ggaatgacag aacaccactt tatcgaaaat gacggctttg tcgctgaaga ttagtactct
     8581 tagcagggta tggtcttgtc aaaatatcaa aaacaattta taataaatag ataccctgaa
     8641 aggaagagaa tcatgaactt agaagaattg aaaaaacgac aggagaagat ccgtaacttc
     8701 tctattatcg cccatattga ccacggaaaa tcaactctag cagaccgcat tttggaaaaa
     8761 acagagacgg tttcaagtcg tgaaatgcag gcccagcttt tggatagcat ggagctagag
     8821 cgggaacgtg gaattactat taagttgaat gccatcgagt tgaattacac tgcaaaagat
     8881 ggggaaactt atattttcca cttgattgac acaccagggc acgttgactt tacctatgaa
     8941 gtttcacgtt cgctagctgc ctgtgagggt gctattttgg tggtcgatgc ggctcaagga
     9001 attgaggctc aaactcttgc caacgtttat ctggctttgg ataatgattt ggaaatcatg
     9061 cctatcatta ataaaattga cctgccggct gcagatccgg agcgcgtgcg tacagagatt
     9121 gaagatgtga ttggtttgga tgccagtgaa gcagttttgg cttctgccaa ggctggtatt
     9181 gggattgaag aaatcctcga gcaaattgta gaaaaagtac cagcaccaac gggtgatgtg
     9241 acggcgccac ttaaggcctt gattttcgac tctgtttacg atgcttaccg tggggttatc
     9301 ctccaagtac gtgtcatgga cggagtggtc aaacctggtg ataagattca gctcatgagc
     9361 aatagtaaga cctttgatgt gaccgaagtc ggtattttta caccaaaagc ggttggtcgt
     9421 gatttccttg cgactggtga tgttggttac attgcggcgt ctatcaagac ggttcaggat
     9481 actcgtgtgg gtgataccgt taccttggca accaatcctg cggcagaacc attacatggt
     9541 tataagcaga tgaatcctat ggtctttgcg ggtctctacc caatcgaatc aaacaagtac
     9601 aatgacctgc gtgaagcgct tgaaaaattg caactgaatg atgctagtct tcagtttgaa
     9661 ccagaaacat ctcaggcact tggatttggt tttcgttgtg gatttcttgg acttctccat
     9721 atggatgtga tccaggaacg tttagagcgt gagttcaata ttgacctcat catgacagct
     9781 ccgtctgtta tttacaaagt taatttgacc gacggtgagt ctatggatgt gtctaaccca
     9841 tctgagtttc cagacccaac taagattgcg accattgaag agccttatgt caaggcgcaa
     9901 atcatggtac cacaggagtt cgtcggagca gtaatggagc tagctcagcg taagcgtggg
     9961 gactttgtga ctatggacta tattgatgat aaccgtgtca atgttatcta tcaaattcct
    10021 cttgctgaaa ttgtctttga cttctttgat aaacttaagt cttcgacacg tggttatgca
    10081 agctttgact acgaattgtc agaatatcgc ccatctaagc tggtgaaaat ggatatcctt
    10141 ctcaatggag ataaggtgga tgccctcagc tttatcgttc acaaggactt tgcctacgaa
    10201 cgtgggaaac tcatcgttga taaactcaag aaaatcatcc ctcgccaaca atttgaagtt
    10261 ccaatccaag cggctattgg acacaagatt gtcgctcgta ctgatatcaa ggcccttcgt
    10321 aagaacgtac ttgctaaatg ttatggtggt gacgtttctc gtaagcgtaa actccttgaa
    10381 aaacaaaaag ctggtaagaa acgcatgaaa tccatcggat cagttgaagt tccgcaagaa
    10441 gccttcctca gcgtcttgag tatggatgaa gaataagtta tcagaaatgc ctttccttgt
    10501 tcacaggaaa tttatataag aatcaatagt aaagcaggct tagatggtct gcttttttag
    10561 tttttatact cttcgaaaat ctcttcaaac cacgtcagcg tcgccttacc gtatgtatgg
    10621 tt
//
LOCUS       DAVKJJ010000064        10358 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000064 DAVKJJ010000000
VERSION     DAVKJJ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10358
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.064"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            <1..185
                     /locus_tag="VW144_001791"
     CDS             <1..185
                     /locus_tag="VW144_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9601203.1"
                     /translation="EYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDTSSSG
                     VTLITGKNGTGKSTLL"
     gene            complement(556..1866)
                     /locus_tag="VW144_001792"
     CDS             complement(556..1866)
                     /locus_tag="VW144_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU9601204.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNTLYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2103..2975
                     /locus_tag="VW144_001793"
     CDS             2103..2975
                     /locus_tag="VW144_001793"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU9601205.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            3106..3950
                     /locus_tag="VW144_001794"
                     /pseudo
     CDS             3106..3950
                     /locus_tag="VW144_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            4011..4973
                     /locus_tag="VW144_001795"
     CDS             4011..4973
                     /locus_tag="VW144_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9601206.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYDDTSISVSNNETDKV
                     LAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAEASKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFMKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVEPLRYE"
     gene            5150..6157
                     /gene="gap"
                     /locus_tag="VW144_001796"
     CDS             5150..6157
                     /gene="gap"
                     /locus_tag="VW144_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU9601207.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHNVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(6224..7060)
                     /locus_tag="VW144_001797"
     CDS             complement(6224..7060)
                     /locus_tag="VW144_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9601208.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWKEIVNPGD
                     VCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETYVSRLKQFSNKTSLARCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            7186..9381
                     /locus_tag="VW144_001798"
     CDS             7186..9381
                     /locus_tag="VW144_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9601209.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEASWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTSGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            9434..9586
                     /gene="rpmG"
                     /locus_tag="VW144_001799"
     CDS             9434..9586
                     /gene="rpmG"
                     /locus_tag="VW144_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9601210.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            9596..9772
                     /gene="secE"
                     /locus_tag="VW144_001800"
     CDS             9596..9772
                     /gene="secE"
                     /locus_tag="VW144_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU9601211.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            9827..>10358
                     /gene="nusG"
                     /locus_tag="VW144_001801"
     CDS             9827..>10358
                     /gene="nusG"
                     /locus_tag="VW144_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU9601212.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAE"
BASE COUNT         3241 a         1788 c         2252 g         3077 t
ORIGIN      
        1 aagagtatga aaacagaact gtttcttttg ctattagttc aaaaggagaa aaaatgaaag
       61 tagaaaatat ttcgtatagg gtggatcatc gtatattgtt tgataatatt tcttttgata
      121 cttcgagttc aggcgtgaca ttaattactg gtaaaaatgg tacaggaaag tcaactttac
      181 tatagtagat tgaaactaga atagtacaca tctacttcta aaatattgtt agaaatcgat
      241 ttgactatcc tgatctattt gtcctgttct tatttcattt catttcacta catctcaaat
      301 tgagtatgac gaagtgcgct cccatgtcct gggaacgcac tttcttcata tttttcatat
      361 tcttgaatcc atcgataaag actattggga tgaattttta aagttgaact aattattttt
      421 acaggatgag gtttacagca gagagtttga aggctttatc aaaggttttt cttggcataa
      481 tgacttttcc tcgtttccac ttaattttgt gtctacttta ttataccaag tccaccctta
      541 agttagataa tgaatctaac ttaaggaagc tagaaggatg agaatccagg tggtcaagag
      601 tcccaaactt aagctgatag ggacacccag aataatttgc tttttgaagg caagaccgcg
      661 ttcctctata ttgggaagtg agagttgaat gagagaacca gctgatgaaa agggtgagat
      721 attagtagat agagcgccaa taacggtggc tgttgtgagt aagtgaatat caatctgagg
      781 attttgagca ctgatgatag caatgatggg aaagagggct ggagctacaa cggatagggt
      841 ggaactaaag agtgacatca ctccggctat cacacaaaag aacagaggta accagaaatg
      901 aggaatggtt gttgtcatga ggtgccctat cagtgtgact aaacctgact tgaccgctag
      961 agacattagt aagctcatgc cgcagagcat gataattgta gcccagggaa ccttagctaa
     1021 aatggcttct tgcttcccta atttgagcct taaggcgagg cagaccatga gtattgagac
     1081 aaagccaata tcaaatgttt tttgataagt agctatccag gcgatgtttg ggaaaatgag
     1141 atgcaacaag ggaaaaagcc aaaccaaaac catgctgctg atcatgagca aggtggtttg
     1201 tctttgaacc ttgctgagga gtggtggttg gtcaatagtc aaggatgagt ttgttcttcc
     1261 cttactatag tgactgtaac aggataataa aagcaagacg atgagtgggt agataatgct
     1321 gacgataaag atatgattgc caagtgaaaa agcttgctct tcccatccca tttgcttaaa
     1381 caggccttga aagacaatgc ctgagctact ggttatcaaa ttagcccctc ctgaagctcc
     1441 ccaattgacg gcttgagctc caatcaaagg gtgtttgtcc gctttttgac agagggtaat
     1501 cgctagagga cagcaaacgg ccatagtagt gaaaaatcca gcacctaaag cagacaaaag
     1561 ggttgccatc aggtataaaa tcatgtagag ggtgttaggg tgggtgcgtg tgcggtagag
     1621 aatgtgttga gccaaaacat caagagtacc gttagttgtt gcaacgttat aaaagagaga
     1681 gacgctaaaa atggtaaaaa agagtgaggt tggccaaaaa tgaagaagtt ctttggggct
     1741 taatcccatg agagtggttg cgatgaggta agaaaaagca atagccagca ggccgatatt
     1801 gattttggtg cggtaaccaa ttccaatggc tagagcaatg gcgctaatca ttattaaatg
     1861 aatcattgaa ttgtcctttc agttagaata tggaaatagg ttggcaattt tggcgctcaa
     1921 aagaccacct gtttcctatt ggtacttgac tattatacac aaaaaaaata caattgtctt
     1981 gacaattgta ttgacacttg ttagaattaa aaatgaggta ttcaggtttg tgatttccta
     2041 tggaagttaa tagtaattgc ctctaatgct tacaagtgat attaaaaata gaggacttag
     2101 tgatgtcaat catttcaact gatttaaccc cttttcaaat agatgataca ttgaaagcag
     2161 ccttgcgaga agatgttcat tccgaagatt acagtaccaa tgccattttt gatcatcatg
     2221 gccaagccaa ggtgtcgctt tttgccaagg aagctggtgt tttagcgggg ctaaccgttt
     2281 ttcaaagggt ttttacccta tttgatgccg aggtgacctt ccagaatcct catcaattta
     2341 aggatgggga tcgtttgact agtggcgatt tggttttaga aatcataggc tcggtgagaa
     2401 gtctcttaac atgtgaacgc gttgccttga attttttaca acatttatca gggattgctt
     2461 cgatgacagc tgcttatgta gaagccttag gcgatgattg cattaaggta tttgatactc
     2521 gaaaaactac tcctaattta cgtctttttg agaaatatgc cgtgagagtt ggcggtggct
     2581 ataatcatcg ctttaattta tcagatgcta tcatgctaaa agacaatcac attgcggcag
     2641 taggtagtgt tcaaaaggca attgctcaag cgcgtgccta tgcccctttt gtgaaaatgg
     2701 tcgaggtgga agtggaaagc cttgctgctg ccgaagaagc tgcggcggcg ggtgctgata
     2761 ttatcatgtt ggataatatg tcattggaac agattgaaca ggccattacc ctaattgcag
     2821 gacgttctcg gattgaatgt tctggaaata ttgatatgac cactattagc cgttttcgtg
     2881 gtttagcgat tgattacgtc tccagtggta gtttaaccca tagtgctaag agtcttgatt
     2941 tttccatgaa ggggttaacc taccttgatg tctaagttgt aaaataaact aactttttaa
     3001 aggatgtctt tcctctagaa cgagttttat gtcagatagt ttaaacgcct cttcaaatat
     3061 agtaaaatga accaaaaata gtacacaatg tggtataatc ttcttatggc atattcaata
     3121 gattttcgta aaaaagttct ctcttattgt gagcgaatag gtagtataac agaagcatca
     3181 cacgttttcc aaatctcacg taataccatt tatggctggt taaagctaaa agagaaaaca
     3241 ggagagttaa accaccaagt aaaaggaaca aaaccaagaa aagttgatag agatagactt
     3301 aaaaactatc ttactgacaa tccagacgct tatttgactg aaatagcttc tgaatttggc
     3361 tgtcatccaa ctaccatcac tatgcgttca aagctatggg ctacactcga aaaaaagaac
     3421 cacacctact atgaacaaga cccagaaaag tagccttatt tcttaagaat tttaatagtt
     3481 taaagcacct agcacctgtt tagattgatg aaacaggatt cgatacttat ttttatcgag
     3541 aatatggtcg ctcattaaaa ggtcagttaa gaagaggcaa agtatctgga agaagatatc
     3601 agaggatttc tttggttgca ggtctaacaa atggtgaatt aatcgctcca atgacttacg
     3661 aagagatgat gacgagcgac ttttttgaag tatggtttca gaagtttctc ttaccaacat
     3721 taaccacacc atcggttatt attatggata atgcaagatt ccatagaatg ggtaagctag
     3781 aactcttgtg tgaagagttt gggcataaac ttttacctct tcctccctac tcacctgagt
     3841 acaatcctat tgagaaaaca tgggctcata tcaaaaagca cctcaaaaag gtattaccaa
     3901 gttgcaatac cttttatgag gctcttttgt cctgctcttg tttcaattga ctatagttca
     3961 cggatacagt tgggaaagaa gctattaaat atttggagag tccttttggg atgaacatgt
     4021 caaatattac tatagatggg caagaaatgg atctgagtca aacgatgcct tcttatgctc
     4081 aagtagtgag tctttatgat gatacaagta tctctgttag taataatgag acggacaaag
     4141 tgttggctgg atccctctat actgatacaa atgaacaggg attaacgatt ccaagcagtt
     4201 tactaaaaaa ttggaatgaa cagacaggaa aaaatttgac agctaatgat cttattggca
     4261 aatcagtctc agccagcatt gtagaaagtg ctgccgaagc tagtaagatt gctcaatttc
     4321 aaacgaagat tgtacgtgta atcaatgatg aagatgacat ggaggacagc aacagtttca
     4381 tgctgtctca tcaaatggaa acgattttga aagaggctgg atttatgaaa gctgtatctt
     4441 attttatctt ggaactcaaa gatccatcac agacaaaagt agtaacagaa gaattacaga
     4501 aaaataagaa gtatactgtg ctttctcaac agagagttct tgatattgtg attaccttta
     4561 ttcgtgttat tcagggatta ttgattgtgc tttcatcaca agctattgtg gtagcagcgg
     4621 ttatgattgg tattattatt tacatcaata tcatgcaacg ttccaaggaa ataggtgtca
     4681 tgaaagcagt tggttatcag aatcgtgatg tcaaaggaat ttttatttac gaggctatct
     4741 ggattgtagg catcgccttg ttgctggcat ttttggttgc acaaggggtg ggaagtttgg
     4801 cgaatgcgat tgtaagtcac ttttacccat ccatcactaa ggtttttgaa ttaaatcttt
     4861 tatctgtttt aggaactcta gttttcgctc tattacttgg ttatgtctca gcctacttcc
     4921 cggcgcgtaa gattagtaaa atggatcctg tagaaccgtt acgctatgaa tagtaaggga
     4981 ttccttgatt tacagtctta aaaagaaact aacatagaaa aacaatggcc atacttgcaa
     5041 ttcaactcaa atagttgtat aataggtttg ttgaaaggtg atttgtagtg attcaagtta
     5101 ctcttttcac aataaaaatt tcatgatttt cataaggagg aaatcactaa tggtagttaa
     5161 agttggtatt aacggtttcg gacgtatcgg tcgtcttgct ttccgtcgta tccaaaacgt
     5221 agaaggtgtt gaagttacac gcatcaacga ccttacagat ccagttatgc ttgcacactt
     5281 gttgaaatac gacacaactc aaggtcgttt cgacggtact gttgaagtta aagaaggtgg
     5341 atttgaagtt aacggtaaat tcatcaaagt ttctgctgaa cgtgatccag aacaaatcga
     5401 ctgggctact gacggtgtag aaatcgttct tgaagctact ggtttctttg ctaagaaaga
     5461 agcagctgaa aaacacctta aaggtggagc taaaaaagtt gttatcactg ctcctggtgg
     5521 aaacgacgtt aaaacagttg tattcaacac taaccacaac gttcttgacg gtactgaaac
     5581 agttatctca ggtgcttcat gtactacaaa ctgcttggct ccaatggcta aagctcttca
     5641 agacaacttt ggtgttgttg aaggattgat gactactatc cacgcttaca ctggtgacca
     5701 aatgatcctt gacggaccac accgtggtgg tgaccttcgc cgtgctcgcg ctggtgctgc
     5761 aaacatcgtt cctaactcaa ctggtgctgc aaaagctatc ggtcttgtaa ttccagaatt
     5821 gaatggtaaa cttgacggat ctgcacaacg cgttccaact ccaactggat cagttactga
     5881 attggtagca gttcttgaaa agaacgttac tgttgatgaa gtgaacgcag ctatgaaagc
     5941 agcttcaaac gaatcatacg gttacacaga agatccaatc gtatcttcag atatcgtagg
     6001 tatgtcttac ggttcattgt ttgacgcaac tcaaactaaa gttcttgacg ttgacggtaa
     6061 acaattggtt aaagttgtat catggtacga caacgaaatg tcatacactg cacaacttgt
     6121 tcgtactctt gaatacttcg caaaaattgc taaataattc ttgagttgat agaaagcaag
     6181 gctttgtggt cttgcttttt atatggaaaa atggatgaca cgatcatcca ttctttttta
     6241 attctttttc aaatgtattt gaaagggtag tgaaagttag cttctctaaa gtaagtgggt
     6301 gggtaaagga aagtcggaag gcatgaagca taagccgact tgtctttgat ttactattat
     6361 agagagggtc gcccaggata ggaagattat gatgcgaaag gtgcacacga atctgatggg
     6421 ttcgccctgt ctttagcttg caacgagcca aggaagtctt gtttgagaat tgctttaatc
     6481 tgcttacata cgtttcagca tattgcccat tttttgcatc aactattctt tttctacgat
     6541 catggcgatc acgtccaatt ttgtctctga aaacaagttc ttttctgttg atatttccat
     6601 caactagagc ccaatattct ctagaaatct cttttttctc caataagcga ttgagaatgg
     6661 gcaggataaa aggatttttg gcaaagagaa ctaagccact ggtttccatg tccagacgat
     6721 gaacgacata gcaggtttgg ccaacatagg tactgacatg gttaagaagg gcaatttcgt
     6781 ttggttgatt accatgcgtt ttcatcccct ctggtttgtt tacaataatc aagtgttgat
     6841 cttgataaac ttcctgcact aagtctgggt tgccccaagg gatcgtcttt tgggaataat
     6901 cttcctcgtc aaaagtcaac tggcaaacat ctccaggatt tacgatttcc ttccagtgga
     6961 cttcttcttg atttatcaaa atatgtttct tgattctcaa aaaatgacgg atttttctag
     7021 ggatgaggag ttgttcctca agtaattgct ttactgtcat ttgaggtaga gaggcgggta
     7081 atgtaaatgt gaattgcata cagatattgt aacaaaaaaa gccctatttg gataggaaat
     7141 agctaaattc ttgtcttcct atgatgaaga tgataaaata aacgcatgaa attagataaa
     7201 ttatttgaga aatttctttc tctttttaaa aaagaaacaa gtgaactaga ggactctgat
     7261 tctactatct tacgtcgctc tcgtagtgat cgaaaaaaat tagcccaagt aggtccgatt
     7321 cgaaaattct ggcgtcgtta tcatctaaca aagattatcc ttatactagg tttgagtgca
     7381 ggcttgctag ttggaatcta tttgtttgct gtagccaagt cgaccaatgt caatgatttg
     7441 caaaatgcct tgaaaactcg gactcttatt tttgaccgtg aagaaaaaga ggctggtgcc
     7501 ttgtctggtc aaaagggaac ctatgttgag ctgactgaca tcagtaaaaa cttgcagaat
     7561 gctgttattg cgacagaaga ccgttctttc tataaaaatg acgggattaa ctatggccgt
     7621 tttttcttgg ctattgtcac tgctggacgt tcaggtggtg gctctaccat tacccaacag
     7681 ctggctaaaa acgcctattt atcgcaggat caaactgttg agagaaaagc gaaagaattt
     7741 ttccttgcct tagaattaag caaaaaatat agtaaggagc aaattctaac catgtacctt
     7801 aacaacgctt attttggaaa tggtgtgtgg ggtgtagaag atgcgagtaa gaaatacttt
     7861 ggagtttctg catcagaagt gagtctggat caagctgcga ctctggcagg gatgctcaag
     7921 gggccggaac tgtataatcc cttgaattcc gtagaagatt ctactaatcg gcgcgatact
     7981 gtcttgcaga atatggttgc agcaggatat attgataaaa accaagaaac cgaagctgct
     8041 gaagttgata tgacttcgca attgcacgat aagtatgaag gaaaaatctc agattaccgt
     8101 tacccctctt attttgatgc ggtggttaat gaagctgttt ccaagtataa tctaacagag
     8161 gaagagattg tcaataatgg ctaccgcatt tacacagagc tggaccaaaa ctaccaagca
     8221 aatatgcaga ttgtttatga aaacacatcg ctatttccga gggcagagga tggaacgttt
     8281 gctcaatcag gaagtgtagc cctcgaaccg aaaacagggg gagttcgtgg agttgtcggt
     8341 caagttgctg acaatgataa aactggattc cggaatttca actatgcaac ccaatcaaag
     8401 cgtagtcctg gttctacaat taagccttta gttgtttata cgccagcagt tgaagcaagc
     8461 tgggctttga ataagcagtt ggataaccat accatgcagt atgacagcta taaggttgat
     8521 aactatgcag ggatcaaaac gagtcgagaa gttcctatgt atcaagcctt ggcagaatcg
     8581 cttaatctac ctgctgttgc cactgttaat gatttgggtg tcgacaaggc ttttgaggca
     8641 ggcgaaaaat tcggactcaa catggaaaag gtcgaccgtg ttcttggtgt cgccttggga
     8701 agcggtgttg aaaccaaccc tcttcaaatg gctcaagcat acgccgcctt tgcaaatgaa
     8761 ggtttaatgc ctgaagctca ttttattagt agaattgaaa atgctagtgg acaagttatt
     8821 gcgagtcata aaaattcaca aaaacgggtg attgataagt ctgtagctga caagatgacc
     8881 agtatgatgt tggggacttt caccaacggt accggtatta gttcatcgcc tgcagactat
     8941 attatggcag ggaaaactgg aacaactgaa gcagttttta atccggagta cacaagtgac
     9001 cagtgggtaa ttggttatac tccggatgta gtgattagcc actggcttgg ctttccgacc
     9061 actgatgaaa accactatct agctggctct acttcaaacg gtgcagctca tgtctttaga
     9121 aacattgcca ataccatttt accttatacg tcaggaagta cctttacggt tgaaaatgct
     9181 tataagcaaa atggaattgc accagccaat acaaaaagac aagtacaaac caatgataat
     9241 agccagacag atgataattt gtctgatatt cgagggcgtg cgcaaagtct agtagatgag
     9301 gctagccggg ctatctcaga tgcgaagatt aaggaaaagg ctcaaacaat atgggattcg
     9361 atagtcaatc tatttcgcta agatgcttgt caaagcctag ctttcttgtt ataatggata
     9421 agatggaggc gttatggcac taaaaaaagc aagcctagct tgtgcggttt gtggttcgag
     9481 aaactattca atcaagatca gcggaaaccc caagcctaca cgactagaag taaataaatt
     9541 ttgtaagcat tgtggcaagt acactacaca tagagaaacg agataggaga gagcgatgcg
     9601 ttttattgga gatattttta gacttcttaa agacacaacg tggccaactc gcaaggaaag
     9661 ctggagagat tttcgttcta tcatggaata cacagctttc tttgtagtaa ttatttacat
     9721 ttttgaccag ttgattgttt caggtttgat tcgatttatt aacatttttt agaagattag
     9781 tggagttaat tacactagaa atcttctatt tatgaaagga aatatcatgg atagttttga
     9841 taaaggatgg tttgttttac aaacttattc tggttatgaa aataaggtga aagaaaatct
     9901 attacaacgt gcacaaacct acaatatgtt ggataatatt ctacgcgttg aaattccaac
     9961 acaaacagtg caagttgaaa aaaatggaaa gagaaaagaa gtagaagaaa atcgctttcc
    10021 aggttatgtt cttgtagaaa tggtcatgac agatgaagct tggtttgttg ttcgaaacac
    10081 accaaatgtt acaggatttg tcggatcaca cgggaacaga tcaaaaccaa ctccattatt
    10141 ggaacaagaa attcgtgaca ttttggtatc tatgggacaa actgttcaag aatttgattt
    10201 cgatgttgag attggtcaaa ctgtacgtat tattgatggt gcttttgcag actacactgg
    10261 taagattaca gaaattgata ataacaaagt gaaaatgatt atctctatgt ttggtaatga
    10321 cacagttgca gaagtaaacc taaaccaaat cgcagaat
//
LOCUS       DAVKJJ010000065        10333 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000065 DAVKJJ010000000
VERSION     DAVKJJ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10333
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.065"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            89..1264
                     /gene="mutY"
                     /locus_tag="VW144_001802"
     CDS             89..1264
                     /gene="mutY"
                     /locus_tag="VW144_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU9601213.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATASEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYVQANLDSSQD"
     gene            1319..2023
                     /locus_tag="VW144_001803"
     CDS             1319..2023
                     /locus_tag="VW144_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9601214.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2016..3365
                     /gene="vicK"
                     /locus_tag="VW144_001804"
     CDS             2016..3365
                     /gene="vicK"
                     /locus_tag="VW144_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEU9601215.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3367..4176
                     /locus_tag="VW144_001805"
     CDS             3367..4176
                     /locus_tag="VW144_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9601216.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4267..4509
                     /locus_tag="VW144_001806"
     CDS             4267..4509
                     /locus_tag="VW144_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEU9601217.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4511..4765
                     /locus_tag="VW144_001807"
     CDS             4511..4765
                     /locus_tag="VW144_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU9601218.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            4812..5507
                     /locus_tag="VW144_001808"
     CDS             4812..5507
                     /locus_tag="VW144_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEU9601219.1"
                     /translation="MKKKWLFADYYDTTIILLALISVILVLLGFAEMTDLDNPPYSII
                     DLVIWGVFVIDYSWRFFITKRKWRFILENIFDLLAILPLNAIFTVFRLGRIFRLARLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDIVPVSLFGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLKDELSS"
     gene            complement(5856..6842)
                     /locus_tag="VW144_001809"
     CDS             complement(5856..6842)
                     /locus_tag="VW144_001809"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEU9601220.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            7036..9504
                     /gene="gyrA"
                     /locus_tag="VW144_001810"
     CDS             7036..9504
                     /gene="gyrA"
                     /locus_tag="VW144_001810"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU9601221.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQILSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLTEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            9504..10247
                     /gene="srtA"
                     /locus_tag="VW144_001811"
     CDS             9504..10247
                     /gene="srtA"
                     /locus_tag="VW144_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEU9601222.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
BASE COUNT         3212 a         1751 c         2303 g         3067 t
ORIGIN      
        1 agtagataga aaagttatat actatctatt gaaaatatta taaatcagat acaaagacag
       61 gtgaaacctt ctgtgttata ctagagatat gttagatttg aaagaatacg gtatcgtcat
      121 gtggccggag gagaaagtca tttctttccg tgagaaactt ctcgcttggt atgatgaaaa
      181 caaaagagat ttgccttgga ggagaagtaa aaatccttat cacatctggg tatctgaaat
      241 catgcttcag cagaccaggg tggatacagt tatcccttac tacgaaagat tcctggactg
      301 gtttccaact gtcgaaagtc tggcaactgc gtctgaggag agtttactga aagcttggga
      361 gggcttgggc tattattctc gagttcgcaa tatgcaggct gcagcccagc agattatgac
      421 tgactttggt ggccaatttc caaataccta tgaaggaatt tccagcttga aagggattgg
      481 accttacaca gcaggagcca tttccagtat tgcttttaac ttgcctgagc cagctgtaga
      541 tggtaatgtc atgcgggtct tggcgcgtct gtttgaagtc aaccacgata ttgggattcc
      601 aagtaatcga aaaatttttc aggcaatgat ggagatcttg attaatccgg atcgtccggg
      661 tgattttaat caagccttga tggacttagg gtctgatatt gagtctcctg taaatcccag
      721 acctgaagaa agcccagtca aggactttag tgcggcatat cagaatggaa ccatggaccg
      781 ttatccaatt aaatctccta agaaaaagcc ggttcctatt tatcttaaag ccttagttgt
      841 gaaaaattct caaggacaat ttttacttga aaaaaatgaa agcgaaaagc tcttggcagg
      901 tttttggcat ttccccttta tagaagttga taacttttcg caagaagagc agtttgacct
      961 ctttcatcag gttgcggaag aaagtgtgaa ctttggtccc agtccagaag agagtttcca
     1021 gcaggactat gacctagatg ttgattggct tgatgtttgt tttgatacag tccagcatgt
     1081 ttttagtcat cgaaaatggc atgtacagat tgtagcaggt caggtgagtg acttccatga
     1141 tttttcagat agggaagttc gttggctttc accagaagaa ttcaagaatt acccacttgc
     1201 caaaccccaa caaaaaatct ggcaggctta tgtacaagcc aacttagaca gtagccaaga
     1261 ctagctattg tgtcttctga ctattttttg gtataatagt agagaaaaag gtgaacatat
     1321 gaaaaaaata ctaattgtag atgatgagaa accaatctcg gatattatca agtttaatat
     1381 gaccaaggaa ggttacgaag ttgtaactgc ttttaatggt cgtgaagcgc tagagcaatt
     1441 tgaagcagag caaccagata ttattattct ggatttgatg cttccagaaa ttgatggttt
     1501 agaagttgct aagaccattc gtaagacaag cagtgtgccc attcttatgc tttcagccaa
     1561 agatagtgaa tttgataagg ttatcggttt ggaacttggg gcagatgact atgtaacgaa
     1621 acccttctcc aatcgtgagt tgcaggcgcg tgttaaagct cttctgcgtc gttctcaacc
     1681 tatgccagta gatgggcagg aagcagatag taaacctcaa cctatccaaa ttggggattt
     1741 agaaattgtt ccagacgcct acgtggctaa aaaatatggc gaagaactag acttaaccca
     1801 tcgtgaattt gagcttttgt atcatttagc atcgcataca ggtcaagtca tcacgcgcga
     1861 acacttgctt gagactgtct ggggttatga ctattttggt gatgtccgta cagttgatgt
     1921 gactgtacga cgtctgcgtg agaagattga agatacgccc agccgaccag agtatatctt
     1981 gacgcgccgt ggtgtagggt attacatgag aaataatgct tgatttactg aaacaaacca
     2041 tttttaccag agattttatc tttatcctga ttttgttagg tttcatcctt gttgtgaccc
     2101 tcttattact ggaaaataga cgtgataata ttcagttgaa gcaaatcaat caaaaggtta
     2161 aagatttgat tgcaggagat tattccaagg ttcttgatat gcaaggtggg tctgaaatca
     2221 ccaatattac caataatttg aatgacttgt cggaggttat tcgtctcact caggaaaatc
     2281 tagaacaaga gagtaagagg ctaaatagta ttctgtttta tatgacagat ggggttcttg
     2341 cgactaaccg tcggggtcag attatcatga ttaacgatac agccaagaag caactggggt
     2401 tggttaagga agatgttctg aatagaagca ttttggaatt gctcaagata gaagaaaact
     2461 atgaattgcg tgatttgatt acccaaagtc cagaattgtt gctagattcc caagatatca
     2521 atggcgaata tttgaacctt cgagttcgct ttgccttgat acgtcgagag tctggcttta
     2581 tttcaggttt ggtggctgtt ttgcatgata cgacggagca ggagaaggaa gaacgcgaac
     2641 gaagactctt tgtttccaat gttagccatg agttacggac tcctctgact agcgtaaaat
     2701 cctatcttga agccttggat gagggggctt tgtgtgaaac tgtagcacca gactttatca
     2761 aggtttctct tgatgagacc aaccgtatga tgcgcatggt gacggatctc ctccatcttt
     2821 cacgtattga taatgctacc agtcacctag atgtggaact gattaacttc actgctttta
     2881 ttacctttat cctcaatcgt tttgacaaga tgaaaggaca ggaaaaggag aaaaaatatg
     2941 agttggtgag agattatccc atcaattcta tctggatgga aattgataca gataagatga
     3001 cgcaggttgt cgacaatatt ttaaataatg ctattaagta ttcgccagat gggggtaaaa
     3061 tcactgtcag aatgaagaca actgaagacc agatgatttt atccatttct gaccacggtt
     3121 tggggattcc taagcaggat ttaccacgta tctttgaccg tttctatcgt gtggatcgtg
     3181 ctagaagtcg tgcacaaggt ggtacaggtc taggactgtc tatcgctaaa gaaattatta
     3241 aacaacataa gggctttatt tgggccaaga gtgaatacgg caagggttca acctttacca
     3301 ttgtgctccc ttatgataag gatgcagtga aagaagaagt atgggaggat gaagtagaag
     3361 actagaatga gtgaaatagg ctttaaatac agtattttag catcgggttc cagtggaaat
     3421 tctttttatc tggaaacctc aaaaaagaag cttttagtgg atgcaggctt gtctggcaag
     3481 aaaattacca gtctgctagc tgaaattaac cgcaagccag aagacctgga tgccatcttg
     3541 attacccatg agcattcaga tcatatccat ggagtaggcg ttttggctcg caagtatggt
     3601 atggatcttt atgccaatga aaagacctgg caagctatgg aaaatagtaa atatcttggc
     3661 aaggtggatt cttcgcaaaa gcatattttt gaaatgggta aaaccaaaac ctttggagat
     3721 atcgacatcg agagttttgg tgtaagccat gatgcagtcg caccgcagtt ctatcgcttt
     3781 atgaaggatg ataagagttt tgtccttttg acagatacag gttatgtcag tgaccgtatg
     3841 gcgggcattg tcgaaaatgc ggatggctat cttatcgagg ccaaccatga tgtagagatt
     3901 ttgcgatcag gttcttacgc ttggcgactc aaacaacgaa tcctatctga ccttgggcac
     3961 ctttctaacg aggacggtgc tgaagctatg attcggacgc taggaaatcg tactaagaag
     4021 atttaccttg ggcatttatc taaggaaaat aatatcaagg aactggctca tatgaccatg
     4081 gtcaatcagc tggctcaagc tgacctggga gtcggagtag actttaaggt ttatgatacc
     4141 tcaccagata ccgcaacacc attgacagag atatagaaag aacgctgaga aggtgttctt
     4201 tttatattga ctgaacaccc aaaaagtaat acaatggtgt taccattaaa aaagggagca
     4261 caaaagatga ctactattac attaaaagtt tctgaagctg ataaaacatt tatgaaagca
     4321 atggctaagt ttgaaggagt ttccctttcg gaacttattc gcaccaaaac tcttgaagcc
     4381 ctagaagatg aatacgatgc tcgtgtggca gatttagcct atcaagagta tttagaagac
     4441 ttggaaaaag gagttgaacc cattacttgg gaagaaatga tgcatgattt aggcttgaag
     4501 gatgaataat ttgtataaat tagttccaac aagacgtttt atcaagcaat tgaaaaaatt
     4561 ggaccgttat acgcagaagc taattacaaa ctatttacaa accaatgttt tggaagaccc
     4621 aagacgacac ggaaaggctt tggttggtaa tcgcgttggt caatggcgct atagaattgg
     4681 taattatcga gttatcgtac aaattgtaga tgatgaatta gttgttgcta ctctagaagt
     4741 tggtcatcgg agagatattt attgaattca agctgagtaa taacagtata gaaagtaagg
     4801 agagtaaaaa tttgaagaaa aaatggttat ttgcagatta ttatgataca acaattattt
     4861 tacttgcttt aatctctgtt attctggtat tacttggatt tgctgagatg actgatttgg
     4921 acaatccgcc ttatagtatt attgatttag taatctgggg tgtttttgta attgattata
     4981 gttggcgctt ttttattact aaaagaaaat ggcgctttat tcttgaaaat atttttgatt
     5041 tattggctat tctcccttta aatgctattt ttacagtgtt tcgattagga cgtatcttcc
     5101 gcttagcaag gctgacaaaa ttactgaaac tgactcgttt acttagaata attggactta
     5161 caggtaaact agaaagaaaa attagtagat tcttacgaac aaatggattg atttatatct
     5221 tgtatgttaa tatctttatt gttctagtag ggagttcgat tttatccgtt gttgaagaaa
     5281 aatccttctc agatagtctt tggtgggctc ttgtgacagt aactactgtt ggttatggtg
     5341 atattgttcc agtttccctt tttgggaaat ggctagcagt tttactgatg cttgtcggta
     5401 ttggaacaat agggatgcta acaagtgctt tgacgaactt ttttgtaaag gataatccag
     5461 atgaacagat aaaacttgat aaactcaaag acgaattatc gagctaaaga atattaatag
     5521 agaaacaatc tgaaaaaatc gaagaactac attgaatgat aaaagactta gttgaaaaaa
     5581 tatcataaag gtgagacaaa ataaaacttg attcgaggag aaatcaagta agttttgaca
     5641 caatagaacg catattatca ggtataaaaa cttgatacta tgcgttttat agagaaataa
     5701 aataaaatct gaacaaatca attaggaatt taaagcattt tataacaacg ttttagagta
     5761 atggggtgct atttcaactt caacctacta taatacagaa aaaaatacag aaaaaaacaa
     5821 ctccctgatg attcaaggag ttgtctatag ttaaattagt ttttagaagc ttcttggaat
     5881 tctggatttt tccatgcttc gtcaataata gcttgcaatt ctttagcaga tgcttgcatt
     5941 ttttgagttt ctgcatcgtt caatggaatg tttactggac gaacgatacc atgtgcacca
     6001 acaacagctg gttgaccgat aaagacattc tcaactccgt attgaccttc ttggaatact
     6061 gaaagtggaa gtactgcgtt ttcatcgtca aggattgctt tagtgatacg agcaagggct
     6121 actgcgatac cgtagtatgt tgcacctttt ttgttgatga ttgtgtaggc tgcatcacga
     6181 acaccttcga acaattcaat caattcagct tcttgaacat tttgagtgtc tttaaggaat
     6241 tcttcaaggt ttacaccagc gatgttagcg tgtgaccaaa cagcgaactc agagtcaccg
     6301 tgttcaccca tgatgtaggc gtgcactgaa cgagcatcca catccaattt ttcagcaagt
     6361 gcttgacgga aacgagctga gtcaagtgaa gtacctgaac cgataacgcg ttctttaggg
     6421 aaaccagaga atttccaagt tgagtaagtc aaaacgtcaa ctgggttagc agcaacaagg
     6481 aagatacctt tgaaaccaga ttcaacaact tgagttacga ttgatttgtt gatagcaagg
     6541 tttttaccta caaggtcaag acgagtttca cctggttttt gaggtgcacc tgcagtgatt
     6601 acaacaaggt cagcgtctgc acagtcagag tattgagctg catagatttt tttaggtgaa
     6661 gtgaaggcaa gggcgtgact aaggtcaagc gcatcaccaa cagctttttc atgcaattgt
     6721 ggaatttcga taattccaag ctcttgtgca attccttggt taacaagtgc aaaagcgtaa
     6781 gatgaaccta cagcaccatc accgacaagg ataacttttt tgtgttgttt agttgaagtc
     6841 attgttttaa acatctcctt aattttatta ggggattttc cctagacaac ttcattctat
     6901 cacttttaaa aaactttgtc acgaatatgc cttatagttc tcgatgtaaa cgttttagtg
     6961 gtttagaggc tgaaatagat gggaatttat ggtataatgt tgttacttac taattgtgaa
     7021 atgaggcatt tattaatgca ggataaaaat ttagtgaatg tcaatctgac aaaggagatg
     7081 aaggcaagtt ttatcgacta cgccatgagt gttatcgtag cgcgagctct tcctgatgtt
     7141 cgagatggct taaaacctgt tcaccgtcgc attctctacg gaatgaatga attgggtgtg
     7201 accccagaca aaccccataa aaaatctgct cgtattacag gggatgtcat gggtaaatat
     7261 cacccacacg gggattcctc tatttatgaa gccatggtcc gtatggctca atggtggagc
     7321 taccgttaca tgcttgtaga tggtcatggg aattttggtt ccatggatgg agatagtgct
     7381 gccgctcaac gttataccga ggcacgtatg agcaagattg ctctggaaat gcttcgtgat
     7441 atcaacaaaa atacagttga tttcgttgat aactatgatg ccaatgaacg ggaacccttg
     7501 gtcttgccag cgcgttttcc aaaccttttg gttaatggag caactggtat cgcggttggg
     7561 atggcaacca atattccacc tcataatctg ggtgaaacca ttgatgcagt gaagttggtc
     7621 atggataatc ctgaagtgac taccaaggac ttgatggaag tcttgcctgg accagatttt
     7681 ccaactggtg ctcttgtcat ggggaaatca ggtatccata aggcttatga aacaggtaaa
     7741 ggttcgattg tcctacgttc tcgtacagag attgaaacga ctaagactgg tcgtgagcgt
     7801 atcgttgtaa cagaatttcc ttacatggtc aataaaacca aggtgcatga gcatattgtt
     7861 cgcttggttc aggaaaaacg cattgagggt atcacagcag tacgtgatga gtcaaaccgt
     7921 gaaggtgttc gatttgttat tgaagtcaag cgcgacgcct cagccaatgt tattctcaat
     7981 aacctcttca aaatgaccca aatgcaaacc aattttggtt tcaatatgct cgctatccaa
     8041 aatggtatac cgaaaatttt gtctcttcgt cagattttgg atgcttatat cgagcaccaa
     8101 aaagaagtgg ttgttcgtcg tacacgtttt gataaggaaa aagcggaagc gcgcgctcat
     8161 atcttagaag gtctcttgat tgcgctagac catatcgacg aagtgattcg tatcatccgt
     8221 gctagtgaaa cggatgcgga agctcaagct gagttgatga gcaagtttaa gctttctgaa
     8281 cgtcaaagtc aagctatcct tgatatgcgt cttcgtcgtt tgacaggttt ggaacgcgat
     8341 aagattcaat ctgagtatga tgacctcttg gctctgattg cggatttagc agatattctt
     8401 gctaagcctg aacgtgtttc tcaaattatc aaagacgaat tggatgaagt taaacgtaaa
     8461 ttttctgata aacgccgtac agagttgatg gttggacaga tcttgagtct cgaggatgag
     8521 gacttgattg aagaatcgga tgtcttgatt accctttcta acagaggcta cattaagcgt
     8581 ttggatcagg acgagttcac tgctcaaaaa cgtgggggtc gtggtgtcca aggaacggga
     8641 gtgaaagatg atgactttgt tcgtgagtta gtgtcaacta gcacccatga tcatctgctc
     8701 ttcttcacaa acaagggacg tgtctatcgt cttaaaggtt atgaaattcc tgagtatggt
     8761 cggactgcca aagggctacc agtagtcaat ctcttgaaat tggatgaaga tgaaagtatt
     8821 cagacggtta tcaatgttga gtctgatcgc agtgatgatg cttatctctt ctttacaacc
     8881 cgtcacggta ttgtgaagag aactagtgtt aaggagtttg ccaatattcg tcaaaatggt
     8941 ctcaaagcgc tgaatttaaa ggatgaagat gagttaatca atgtcttgtt gacagaagga
     9001 gatatggata ttatcattgg taccaagttt ggttatgcag ttcgctttaa tcaatcagcc
     9061 gttcgtggta tgagccgtat cgccactggt gtgaaaggtg ttaaccttcg tgaaggagac
     9121 acagttgttg gtgccagctt gattactgat caagatgagg ttcttattat cacagaaaaa
     9181 ggatatggta agcgtacagt cgctactgaa tacccaacaa aaggtcgtgg tggtaaggga
     9241 atgcagacag ctaaaattac cgaaaaaaat ggcttgctgg ccggtcttat gactgttcaa
     9301 ggtgatgagg atttgatgat tatcactgat acaggtgtca tgattcgaac caatcttgcc
     9361 aatatttcac aaacaggacg tgcaactatg ggagttaaag taatgcgcct ggatcaagat
     9421 gctcagatag tgactttcac aacggttgcg gtggcagaaa aagaagaagt tgggacagaa
     9481 aacgaaacag aaggtgaagc ataatgtctc aaaaaaataa taaaaagaaa aacaagcgaa
     9541 aaaatctgct gacaaatatc ctagcaggat ttctgatatt actgtcactg gctttgattt
     9601 ttaatactca aattcgaaat attttcatag tctggaatac caataagtat caagttagcc
     9661 aggtatcaaa agaaaaatta gaagaaaatc aggatacaga aggcaatttt gactttgatt
     9721 ctgtcaaagc tatctcttcg gaagctgttc taacttctca atgggatgct caaaaattac
     9781 cagttattgg gggaattgca attcctgaat tggaaatgaa tttgccgatt tttaaaggac
     9841 ttgataatgt taatctcttc tacggagctg gtacaatgaa acgcgagcaa gtaatgggag
     9901 aaggaaatta tagtctagct agtcaccata tctttggtgt tgataatgct aataaaatgt
     9961 tattttctcc tttagataat gctaaaaatg gcatgaagat ttatctaacc gataaaaata
    10021 aagtttatac ttatgaaata cgtgaagtca aacgtgtgac accggatcgt gttgatgaag
    10081 ttgatgatag agatggggtc aatgaaatca cattagtaac ctgtgaagac cttgctgcta
    10141 cagaacgtat tattgtcaaa ggtgatttga aagaaacaaa agattattca caaacatctg
    10201 atgaaatcct aacagctttc aatcaaccat ataaacaatt ttattaatac aaatcagtga
    10261 aatcatggat ttcactgatt ttttaagatt ttcataaaaa tatagtaaaa tgaaataaga
    10321 ataggacgaa tcg
//
LOCUS       DAVKJJ010000066         9472 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000066 DAVKJJ010000000
VERSION     DAVKJJ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9472
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.066"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            <1..104
                     /locus_tag="VW144_001812"
     CDS             <1..104
                     /locus_tag="VW144_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9601223.1"
                     /translation="ERNISWIELTRKMFAGEFHYPSELNRLYQKIRH"
     gene            197..784
                     /locus_tag="VW144_001813"
     CDS             197..784
                     /locus_tag="VW144_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9601224.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGVEFVSLLIISLCFSLIHAQGSTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHTGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNLLVYSLLLF"
     gene            986..1123
                     /locus_tag="VW144_001814"
     CDS             986..1123
                     /locus_tag="VW144_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HEU9601225.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
     gene            1413..3911
                     /locus_tag="VW144_001815"
     CDS             1413..3911
                     /locus_tag="VW144_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEU9601226.1"
                     /translation="MNNDEWVYQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICH
                     EMRIPFKHLSTEDKFIMRNGKLVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGP
                     YILSDKRWDEAPIYLRYGVFRPSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVP
                     PDFLNKWLDKKDKKQGDFPFIIDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLG
                     FDGTYSSEKLASECKALKILNEWSETPKIYWHGKIWEHTFLGIEYMKGVPLNRWVTNN
                     FPLYEVVDKTKDYLLRVSKIVEKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIID
                     WEQAVFSNDEKVVHKVAAPGFRAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNY
                     VSQTRIEGKKLFESLGYTREHIDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIE
                     SEQDIQDFIIKLLRGFTLTYGQWRKEFQSRFFPVHYYGLNFNQGIAFSDLAILWSYQQ
                     LAKKVKNFKFDDYYEIRTQVINEAVNNFKKSSLSGLFDGKIGTIWLIYEFGEIDRAVE
                     LFTTHFIEIFENSQNKNLYSGQAGILLVGLYFLSKGEIDNKLGEEILIRLREYTLNYI
                     ENPETFCKVGASDVQSNDPYENFGGLLYGHAGVAWLFGEAYKLTGESIYKNGLELAVD
                     KELVAYKVDSNNSLQYSQGHRLLPYLATGSAGLLLLINRNKEILSSKYLKYLTSLERA
                     TDVVFCVLPGLFNGFCGLEVANNIYSDIDDNFSGQKKLIEQLYRYLCVIEEGFVIAGD
                     NGLKITTDIASGFAGVAIGLVSIMDNKLTILPQI"
     gene            3944..5884
                     /locus_tag="VW144_001816"
     CDS             3944..5884
                     /locus_tag="VW144_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU9601227.1"
                     /translation="MNQSNEIFLNTIQEKTHYKRSISSSFQELKKWNSLIFRRGRYWY
                     QFDNWSIPNTGVIKEWYVEDNDWIPKVSSSSLDNSNSLKIVFDVEKYTLPDDSILQDV
                     RISSDESYIVLVVSSLRSTNLIGIKKYTMEELFIIEDISVESSFYLGKFGVMYTRSKE
                     YGRPSKLFYKSFDSFTEEELFEENECSFRLKIVHIDSNNCFVKSVDFQKGRIFLYSFD
                     RTGFVRHSYTETVAPTPRDIALFSTEKAQYFLGLSSTEEKKDQLILRETSSGDRVSIT
                     IPYQDRARRVHCIGRYILLDCSNAANSVFYLATFKNNSLRELSVNKITFDEKTTLYEN
                     SFSDRVLLFLERRTFYEKILSFDLIENTLKTEFERPIIKSNNTKYFSKVIWTKKDSYD
                     VSIPISLFWKSEDTDELPRRKKCILSVYGAYGKNDNSDLDEIMLSIIDAGFIYAIVHV
                     RGGGYLGGEWYRSGKALNKWNSIRDFIEGVNYLRENDVIDSKRLGLITSSAGGIIAGA
                     VLNEEKNLLQSILLFSPFINPYDTLQNPNDPLSKTEIAEWGDIRDPEVKAYIKSYSPM
                     QNIEKARDSNTVIVNILGEKDPYINNNEVIEWSKKLNSIGVKSLLYLNKAAGHGGFTP
                     SDVLLMIDTLNYFFEEVGRNNL"
     gene            5881..7488
                     /locus_tag="VW144_001817"
     CDS             5881..7488
                     /locus_tag="VW144_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9601228.1"
                     /translation="MNYLKFIKKTKLILMGILIFLSSFNGVLLSGIIVYAGSLNQTSS
                     FSDVLRFGAISILGWSAIYISNYYLEVTEASITKDINVKIKQGYFREQYLSSEMVKDY
                     SSIISVLSNDLRLIEENYFRQIFEIISSILLFIVSLSFMLYLNFLVSIIFIVLSALPI
                     IVPVFMKKMLSNSANEYSNSNAEYTHIIKEIFNGFKTLKSYSVTKEIISLSDKKLDKL
                     EDSTFNLKRSEVLSKLVAVLISGFCFLVPLVVGCYFVIYHKSLSFSELIGIFLANDKV
                     LGPIQSIAYSLNKINTTKDLRKPFLKYLSGEKNFIDAEHDNNGLYTSSIDEIHMKDVV
                     YSITPENKLSIDFSFKSPFRVLLTGTSGSGKTTILNLINGSLKPQKGYVNLLSHGKKS
                     SDSIPTVDQTPYIFDTTIRENVTLFQNEYFSDDQIIEVLKKVNLYEELEKIDILNYQC
                     GENGSNLSGGQKQKIALARALIRNNKVYLFDEISANLDNDNSNSIHDILFNLGISFIE
                     VSHHYDLNDKRYTDIYKLENGTLFKIK"
     gene            7503..8243
                     /locus_tag="VW144_001818"
     CDS             7503..8243
                     /locus_tag="VW144_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9601229.1"
                     /translation="MDKDYILKVKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILG
                     ISGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIP
                     SLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAV
                     IADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILK
                     DGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            8246..>9472
                     /locus_tag="VW144_001819"
     CDS             8246..>9472
                     /locus_tag="VW144_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9601230.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASRLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVIIYQLSPFIQSCFIKLLSIIIFRNNFMFIVSKWNLLYCKSY
                     IKSISAAITGAILLISSFQMISQNILSQFQDDSDLELKVAFIVYVGAPILIVLANIIS
                     IAFLSSHQERIEIQQFEILGTSNYQMVKIKVGEAIFLTFVTSLIAFLLNIKIMALIYY
                     SLEDILIDDMNLLGLIL"
BASE COUNT         3232 a         1186 c         1809 g         3245 t
ORIGIN      
        1 ctgaaagaaa tatcagctgg attgagttaa ccagaaaaat gtttgcggga gagtttcact
       61 atccaagtga attgaatcgt ttgtatcaaa agattcgcca ctagaagatg gaacaacgaa
      121 tgcctcaaag ttcatgggta gaaaggattg tgcaggtatt agatttagat tatgaagatt
      181 tatttcggag gtaactatga aaagaataat tccagtttat atattccaac aagtaaatgt
      241 cctattggta tctctatact tattgaaatt tctttgtatc ggtgagttaa ccatactaca
      301 gattctctat ggttcgtcac tcatttcttt tttatggatg tatggccaac gcaaacaagc
      361 gcataaggtc aatatgaaat ctaggatgaa atggcttggt gttgaatttg ttagcctact
      421 gattataagt ctatgtttta gtctgattca tgctcaagga agcacgaatc aagcaaactt
      481 aattggcctt caacatcaaa ttccttggtt ttcattttta ttgttcttaa tcaatgcgag
      541 tatggttgaa gaatttctgt atcgagaaat tttatggaat ttggtcagaa aattagatat
      601 cagagttgct ttgacaagtg ttttatttgc cttagcacat catacaggaa ccattctagc
      661 ttggtgtttg tatgtttcac ttgggatgtt tttaggtatg gtgcgctata aatctgactt
      721 atggggcagt atgggactac atttggtgtg gaacctacta gtttatagtt tgctgctttt
      781 ttaaaatagg tgacctgatt tttaggtaac ttctaaaact aacttccagt tttgggagag
      841 agatggaagt tactttgaaa agttacggaa acattatttt ctatgtgaca acattgtata
      901 attgcttgca ttttactggt ggagagagta taatgagggt ggataaagta cttaatccca
      961 aaaatttaaa taggaggagc tagctatggc agaagaagta ttaaatcttc aattagtatc
     1021 tgttcaagtt gacgaaacag atgaagttga tggaatgcga tttagtacat tcagtacaaa
     1081 tcgttgcggt aactggagtg cttttagttg ggaaaattgt taattcattt aaattgtaaa
     1141 ctttagagat tctctggagt tatggatgtc gttccaatat gtgcacgttg gaatgttagt
     1201 gcttatatgg ggagaatcca aaggattctc cctttttatt taaataagtc tgtctagcta
     1261 tagatatatt aagtttgata aaatttcttg gagattttgt tatggattat aatttcaatt
     1321 tggagcatcc tttctttttc actaataatg attatagtac agatactagc ataaagtacc
     1381 aagtaagtct gccttttaat tggcacgaag taatgaataa tgatgagtgg gtttatcaat
     1441 accctattgg aaaatttgtt gaaagacaag ggtggaaaat acacattagt tcagagtata
     1501 acagttcaca tgaattgtta caagatgtag caaaaatttg ccatgaaatg cgaattccat
     1561 ttaaacatct tagtacagaa gacaaattta tcatgcgaaa cgggaaattg gttagtcgag
     1621 gattttctgg taaatttatt acatgttatc caaatcaaaa tgaattagag tcggttttgc
     1681 aaaggttaga aagtgcctta aagcaatata atgggccata tattttaagc gataaacgtt
     1741 gggatgaagc accaatatat ctgaggtatg gtgtgtttag gccgtcaagg gatgatgaaa
     1801 agaaggttgc tattgatgaa ttaattgttg gcgatgaagt tgttaaagat gaacgtttac
     1861 cagtatttaa aattccaaaa ggtatagtgc cccctgactt tttgaataaa tggcttgaca
     1921 agaaagataa aaaacaggga gactttccct ttattataga taatgcaatt agattttcaa
     1981 acagtggtgg gatatataat gctaggctaa aagaagatgg gaaaaaaata attttaaaag
     2041 aggctagacc gtatacaggt ctaggatttg atggtactta ttcttcagag aaattagctt
     2101 cggagtgcaa agcactaaaa attctaaatg aatggagtga gacgcctaaa atatactggc
     2161 acggtaagat ctgggagcac accttcttag gaattgaata tatgaagggc gttccactaa
     2221 atagatgggt taccaataat tttcctttat atgaagttgt tgataagacc aaagattatc
     2281 tattgagagt ttcaaagata gtagaaaaac tcatcgattt aacgaataag tttcattctg
     2341 aaaatgtata tcatcaagat ttacatttag gaaatatctt ggttaaggat gaagatgaga
     2401 ttagtataat agactgggaa caggctgtct tttcaaatga tgaaaaagtt gtacataaag
     2461 ttgcagcccc tggttttaga gcatggcgag aaacacttcc cagtgagata gattggtatg
     2521 gtattagaca aatagctcat tatttataca tgccactagt aactacatcg gatttgacat
     2581 acaactatgt aagtcaaacc aggatagaag gaaaaaaact atttgagtct ttgggttata
     2641 caagagaaca tatagattat gtagaaagtt tattgtcata tctagattct aaatgtccac
     2701 agattgagaa tatttccagg aaaaaagttt taaaacccat gcatgagatt agaactatcg
     2761 agagtgagca agatatacaa gattttatta taaaattatt gagagggttt acgctaactt
     2821 atggacagtg gcgtaaagaa tttcaatcaa gattcttccc agttcattat tatggtctga
     2881 attttaatca aggaattgcg ttttcagatt tagctatcct ttggagttat caacaactag
     2941 ctaagaaagt aaaaaatttc aagtttgatg actattatga gattcgaaca caggttatta
     3001 atgaggcagt aaataatttt aagaaatcgt ccctttctgg tctttttgat ggtaaaattg
     3061 gtacaatctg gctcatttat gaatttgggg agatagatcg ggcagttgaa ctttttacaa
     3121 ctcattttat agagatattt gagaattcac aaaataaaaa tttatatagt gggcaggctg
     3181 gaattttact ggttggactg tattttctta gtaaagggga aattgataat aaactaggag
     3241 aagaaatact aattaggctt agagaatata ctttaaatta tatagaaaat ccagaaactt
     3301 tttgtaaagt tggcgctagt gatgttcaga gtaatgatcc ttatgagaat tttggtggat
     3361 tactatatgg tcacgcaggg gtagcttggc tcttcggtga agcttataaa ttaactggtg
     3421 aaagtatcta taaaaatggt ctagaattgg ctgtagataa agaattagtt gcttataaag
     3481 tagatagtaa taattcattg cagtacagcc aaggtcatag attactacca tatctagcaa
     3541 ctggaagtgc tggcctgctt ttacttatta atagaaataa agaaatcttg tcaagcaaat
     3601 acttaaaata tttaacttct ttagagaggg caactgatgt agttttttgt gtattaccag
     3661 gtttatttaa cggattttgt ggtcttgaag ttgctaataa tatatatagt gatatcgatg
     3721 ataacttttc aggacaaaag aaattaattg aacaacttta tagatatttg tgtgtaattg
     3781 aagaaggatt tgttattgcg ggagataatg gtttaaagat aacaacagat attgcttcgg
     3841 gatttgcggg tgttgcaatt ggattggtat caataatgga taataaattg acgattcttc
     3901 ctcaaatata atatttagat accattatta aggggtcata cttgtgaatc agagtaatga
     3961 aatattttta aatacaatac aggaaaaaac acactataag aggtctatct cttcatcatt
     4021 tcaggaatta aaaaaatgga attctttgat atttaggaga gggcgatatt ggtaccaatt
     4081 tgataattgg agtataccca atactggtgt aataaaggaa tggtatgtcg aagataatga
     4141 ctggattcct aaggttagtt cttccagttt agacaattca aattcactta aaattgtctt
     4201 tgatgtggaa aaatatactt tgccagatga tagtatctta caggatgtta ggatttcatc
     4261 tgatgagagt tatatagttt tggttgtatc ctcactgcgt agcactaatt taattggaat
     4321 aaaaaaatat actatggaag agttatttat cattgaagat atttcagtag aaagttcgtt
     4381 ctatttgggt aaattcggtg ttatgtatac tcgctccaaa gagtacggga ggccaagtaa
     4441 gttattctat aaatcttttg atagctttac agaagaggaa ctgtttgaag aaaacgaatg
     4501 ctcttttcga ttaaaaatag tccacattga tagcaataat tgttttgtaa agtcagtaga
     4561 ctttcagaaa ggcaggattt ttctatacag tttcgaccgg acgggttttg ttagacactc
     4621 ttacacagaa acggtagccc caacacctag agatatcgct ctatttagta cagaaaaagc
     4681 acagtacttt cttgggctct cttcaactga agaaaagaaa gatcaactaa ttctaaggga
     4741 aacttcttcg ggagacagag tttctattac tataccatat caagataggg ctagaagagt
     4801 tcactgtata ggacgttata tccttttaga ctgttcaaac gcagcgaatt ccgtctttta
     4861 tcttgctaca tttaaaaata atagtttgag ggagttgagt gttaataaga taacatttga
     4921 tgaaaagaca acactttatg aaaattcatt ttctgatcgt gttcttctgt ttttagagag
     4981 gagaactttt tacgaaaaaa tacttagttt tgatttaata gaaaatacat taaaaactga
     5041 atttgaaaga cccatcataa aatcgaataa tactaaatat ttttctaagg tgatttggac
     5101 taaaaaagac agttatgatg taagcatacc gatttcttta ttttggaaaa gtgaagatac
     5161 ggatgagctc ccaagaagaa aaaaatgtat tctaagtgtc tatggggctt atggtaagaa
     5221 tgataattct gatttagatg aaattatgtt atctataata gatgcaggtt ttatttatgc
     5281 tatagttcat gtaagaggtg gaggctattt gggtggtgaa tggtatcgct caggaaaggc
     5341 gttaaacaaa tggaattcta tcagagattt tattgaggga gtaaattatt taagggaaaa
     5401 tgatgtaatt gacagtaagc gattaggttt aataacttct agtgcaggtg gaataattgc
     5461 tggtgcggtg ttgaacgagg aaaaaaattt attgcaaagc attctcttat tttcgccatt
     5521 tataaatcct tatgatacac ttcagaatcc aaatgatcct ctttctaaga ccgaaatagc
     5581 agagtgggga gatattagag accctgaagt aaaggcttat ataaagtcat attcgcctat
     5641 gcaaaatatt gaaaaggcac gagactcaaa tactgttata gttaatattt tgggtgagaa
     5701 agacccatat attaataata acgaagtgat agagtggtca aagaaattaa attctattgg
     5761 agttaaaagt ttgttgtatt taaacaaagc agctggtcat ggaggtttta ctccatcaga
     5821 tgttctctta atgattgata ctttaaatta tttctttgaa gaagtgggaa ggaataactt
     5881 atgaattatt taaaatttat aaaaaagact aaacttatat taatggggat tttaatattt
     5941 ctatcttcgt ttaatggtgt cttgctctca ggaattattg tatatgcagg tagtttaaat
     6001 cagacttcct cattttctga cgttctaaga tttggtgcta taagtattct gggttggtca
     6061 gcaatctata tatcaaatta ctatttagaa gtaacggaag catcaataac taaagatata
     6121 aatgtaaaaa ttaaacaagg atattttaga gaacagtatc tttcttctga aatggttaaa
     6181 gattactcat ctattatttc agttttgtca aatgatttga gattaatcga agaaaactat
     6241 tttagacaaa tttttgaaat aatttcttca atattgttgt tcatcgtctc tctaagtttt
     6301 atgctttatt taaacttcct agtttcaata atatttattg tattatcagc attgccgatt
     6361 atagtacctg tctttatgaa gaagatgttg tctaattcag ctaatgagta ctctaacagc
     6421 aatgcagagt atactcacat aataaaagaa atttttaatg gttttaagac attaaaatct
     6481 tattctgtta ctaaagaaat aattagtttg tcggataaaa agttggataa actagaagat
     6541 tctactttta atttgaaacg atcagaggtt ctttcaaaat tggttgcagt actaatttca
     6601 ggtttttgct ttctagttcc cttggttgta ggatgttatt ttgtaattta tcataaaagc
     6661 ctttctttta gtgaactgat aggtattttc ttagcaaacg ataaagtttt agggccgata
     6721 caatcaattg cctattcatt aaataagata aatacaacca aagatttaag gaaaccgttt
     6781 ttaaaatact taagtggaga gaagaatttt atagacgctg aacatgataa taacggactg
     6841 tatacttcat caatagatga gatacacatg aaagatgttg tatattctat tacaccagaa
     6901 aataaattaa gtattgactt ctcatttaag tcaccattta gggtattatt aacaggaact
     6961 tctggtagtg ggaaaacaac gattttaaat ttaattaatg gttctttaaa gccacaaaaa
     7021 ggttatgtaa atttgttatc acatgggaaa aagagttcag attcaatacc aacagttgat
     7081 cagacaccat atatttttga cactactatt cgtgagaacg taactttatt tcaaaatgaa
     7141 tatttttcag atgatcagat aattgaggtg ttaaaaaagg taaatctata tgaagaatta
     7201 gaaaagatag atatactaaa ttatcaatgt ggtgaaaatg gtagtaattt gtctggaggt
     7261 caaaaacaaa aaatagcttt agctagagct ctgattagaa ataataaagt gtacttattt
     7321 gacgaaatat cagctaattt agataatgat aattcaaatt ccatacatga tattctgttc
     7381 aatttaggta tttcatttat tgaagtttca catcattatg acttaaatga caagagatac
     7441 actgatatat ataaattgga aaatggaaca cttttcaaaa ttaagtgatt ggaggcaaga
     7501 atatggataa agattatata ttaaaagtga aagggctgta tcatcaattt ttactaggaa
     7561 ataataaaac gttgcaagtg ctgaaaaatg tttctctttc tgcttcgaga ggagaattta
     7621 taagtattct aggaattagt ggttctggaa agtcaacttt attaaaatgt atttctagtt
     7681 tgcttgaacc aacaagtggg gaagtaattt taaatggaat caacccctat aaaatcagaa
     7741 atgcaaaatt gtcaagtata agacgtaacg aagtatcttt tatatttcaa gcatacaatt
     7801 taataccttc cctgccggta atagaaaata tagcacttcc tttgcgatta tcacaaaaaa
     7861 aattaactat taaaaatgta gaaaacttac tcaaaagaat gaagtttaat gctggcttaa
     7921 acgattttgt tggaactctg tctggtggag aacaacagaa ggttgctata gctagagcgg
     7981 ttattgctga tagtgatata atatttgctg atgagccaac tggggcttta gacagcgttt
     8041 ctcgtgaagt aatttttgaa ttattgagag agttagtagg ggcgggtaag tgtgtaatta
     8101 tggtaacgca cgatatagaa ttggcctcga aaactgatcg tgcattaata ttgaaagatg
     8161 gaaaaatttt caaagaactt catagaccta gcggggaaga gttgtataaa atcttagagg
     8221 tacaatcaac tacggaggaa tagagatgct cagattagtt tactatcaat ttttacataa
     8281 taagaaacaa tggttaggcg tttcaccagt aatttttgtt tcgagtttag ttatgggatt
     8341 ggctgttaat ggggtaatta atgttgagaa taattcgcaa gtatttgtcg gacttccaga
     8401 tcctaaacca atttttatgt ttccgatagt attcggagga gtaacattat tctttgtgct
     8461 ctctaacata ataaatatgt tggttgaaat ttttagagat gattacgaac ttctggaggt
     8521 gttgggagca agtcgccttc aactatcatt tcttgtgggg ggacagattt ttatcatttc
     8581 ctcgattatt agttttattg catatctatg ctctatattt gttacgtcaa attattatta
     8641 ttttttacaa tactttttcg gggaaaatat attacctgat atccaatttc aaacgagtgc
     8701 tgttggatgt atcattacag tagtgttgat ttcattttta gcctttctaa gtggttgctt
     8761 ttatacgttc aagaagatta gaaataggaa gagttctaaa ataaggcatg ttctttcaat
     8821 agtaaaaaga atactgctat tggctggttt tagtgttatc tggttgcttt ctttgcaaca
     8881 aattttccaa gatagcacaa ttttagcgaa ggcccagata atattcaata ttgtaatctt
     8941 agatattgtg attatttatc aattatctcc ttttatacag tcatgtttta tcaaactact
     9001 ttctataatt atatttagaa ataattttat gttcattgtt tcgaagtgga atttactata
     9061 ttgtaagtct tatataaaat ctatcagtgc tgcaattaca ggagctatat tactaatttc
     9121 tagttttcaa atgatttctc agaatatact atctcaattt caagatgaca gtgatttaga
     9181 gctaaaagta gcatttattg tctatgtagg agcaccaatt ttgatagtac ttgcaaatat
     9241 tatttcaatt gcatttttgt catcgcatca agaaaggatt gagattcagc aatttgaaat
     9301 tttaggaact tctaattacc agatggttaa gattaaagtt ggtgaggcta tatttttgac
     9361 atttgttact tcattaattg catttttact taacattaaa attatggctt taatatacta
     9421 ctctttagag gatattttaa tagatgatat gaatcttttg gggttaatac tt
//
LOCUS       DAVKJJ010000067         9203 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000067 DAVKJJ010000000
VERSION     DAVKJJ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9203
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.067"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(552..1400)
                     /gene="dprA"
                     /locus_tag="VW144_001820"
     CDS             complement(552..1400)
                     /gene="dprA"
                     /locus_tag="VW144_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEU9601231.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPASILDGLSDGCHHLIQEGAKLLTSGQDV
                     LAEFEF"
     gene            complement(1497..2186)
                     /locus_tag="VW144_001821"
     CDS             complement(1497..2186)
                     /locus_tag="VW144_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU9601232.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(2198..3076)
                     /locus_tag="VW144_001822"
     CDS             complement(2198..3076)
                     /locus_tag="VW144_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU9601233.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(3066..3935)
                     /gene="licA"
                     /locus_tag="VW144_001823"
     CDS             complement(3066..3935)
                     /gene="licA"
                     /locus_tag="VW144_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEU9601234.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGADFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            complement(3952..4974)
                     /locus_tag="VW144_001824"
     CDS             complement(3952..4974)
                     /locus_tag="VW144_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9601235.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPESEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFKNAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4979..5686)
                     /locus_tag="VW144_001825"
     CDS             complement(4979..5686)
                     /locus_tag="VW144_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU9601236.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIENIIEAIDAYRPL
                     TSEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITGIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            6022..7509
                     /gene="tacF"
                     /locus_tag="VW144_001826"
     CDS             6022..7509
                     /gene="tacF"
                     /locus_tag="VW144_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEU9601237.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYSYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFMYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7519..8322
                     /locus_tag="VW144_001827"
     CDS             7519..8322
                     /locus_tag="VW144_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9601238.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     INLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8324..9133
                     /locus_tag="VW144_001828"
     CDS             8324..9133
                     /locus_tag="VW144_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9601239.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKS"
BASE COUNT         2887 a         1855 c         1550 g         2911 t
ORIGIN      
        1 taatctctac ttttgaagaa tttatttttc cttactaaca agatttgaga tggtaggaat
       61 cattgaacat aacctagcca acatcaatca caatcatttc tcctttctca attacactaa
      121 attatagtgt attgaatcta taacagtgca ccttggctgc taaaatattt ctataaatta
      181 atttgacttt cctgatagag ttgttcacat cttatttcaa ttcactatac tttcccttat
      241 actcaacgaa aatcaaagcg caaactaaga agctagccac aggttgctca aagcactgct
      301 ttgaggttgt agataagact gacgaactca gctcaaaata ctgttttgag gttgcagatg
      361 gaagctgacg tagtttgaag agattttcga agagtattat atgagaaaaa tcctctactc
      421 tactaatcca cttagaaaaa acgcatatta gcaagtgtta aaaacttgct aatatgcgtt
      481 ttattatgga aatgttgggt tcattttctc ttaaaattga ttttttctta gaattctagc
      541 ttaggtcatt tttaaaattc aaattccgca agaacatctt gcccactggt gagcaatttt
      601 gctccttctt gaatcaaatg atggcaaccg tctgatagtc catctaaaat gctagcagga
      661 atagcaaaga catcgcgtcc ttcttccatt gctcgctcac acgtaatgag actacctgaa
      721 cgcatcttag cctctgctac aatcacacca cgacaaagtc cagcaatgat gcgattacgg
      781 gcaggaaaat gaaatttcag aggttgttcg ccaggtccat attcacttag aaccagatgg
      841 tcattgccga tgtagtcttg caagcgttta ttggctttag gataaaacac atccagtcct
      901 gttccaatca ctgcaatggt ttttccgcca ttctgaagag ctgccatatg agctgctgtg
      961 tcaatgccct tggccagacc actgacaata accagttcat tttccaagcc ttgaatgact
     1021 ttttcaactg acttagctcc ctgtttgcta caagcacgac tgcccacgac cgctaccttc
     1081 gggaatttca ggaggtcaag atttcccttg taaaataaaa gtacaggcgc atcatatatt
     1141 tcactcaaat cccaaggata acagtcatct aaaatagaga aagatggaaa tttttgaaac
     1201 tctttcgaca aatgcgcatc gtctatctga aaataacgtt ccataaaaac ggctggatta
     1261 cggcaacctg atatatctgc aatatcaccc aacaaaagct cctgatcaac attttcaccg
     1321 tattctagca ctttcaaaat ctgttgattg gtcaaacctg atttttttaa cttatagatt
     1381 tcatagtttg tgattttcat aaataactcc atttcttttt ctactcatct atctattcgt
     1441 aaaagttcgt aaaaaatcaa aaaacatccg actattttgt cagatgttgg aagcttttaa
     1501 ttttcgtttt taagaatttc ttctaattta cgatagtctt ggacactatc gatctcataa
     1561 atgctattgc cttctaattc ttcaacatag acatctagct ctttgatatt atccttaacc
     1621 atattgtccc aatagagatc aacaaattca ccacttgcat aagccttgtc gataaagctg
     1681 acaatctttt ctgcagttgg agcatcccag aaggatacac cactaaggat gcgacctgcc
     1741 ttgctatcaa caataatgtc ttgaaccttg tagtcatctc catagaccaa gaaccattcg
     1801 ttggtacaat cttcacgata aacactaaaa taagtcgaac gtgtcaaatc attgcggaac
     1861 atatttttaa agagataatt gtcagcatca ataacatagc tgttggccaa ttcttctttt
     1921 acaagataga gagagtaaaa gttattgtag tcagcgtatt tatcattgaa aacgaggcga
     1981 acaccgtatt tttcttttaa gtaatcgaat tgttctttaa gataaccaac aatgatgatg
     2041 atgtcattga ttcctttttc tttgagaaac tcaatttggt attcaatcaa aggtttttga
     2101 ttaacctgaa ccaaggcttt aggggtattt tcagtcatag gacgcaagcg agttcccaat
     2161 cccgctgcta agataatggc tttcacacga atctccttta ttctttaata ataatataaa
     2221 ctccagcaat gacgacaagt gacgtcataa tggtcagcat atctagcggt gcacccaaaa
     2281 aaacaactgc aaacaagacc gtccatacta catagctcac gttcaagcct gtagccttgg
     2341 ctggttgcaa gcgattgata gcgatataat aagccaagta ggaaatcata tcaaaggctg
     2401 caaaaacaat catgagacct agcaattgtc cattggctac tgcagtaaat gactgatgag
     2461 agaagagcac aatcacaaga taggacaaga acgaagtcac ttgacggatt aagagggctt
     2521 cgatttcact cagttcactt tccatagcaa aggagctaag aacactctca cttccccatg
     2581 caatagcaca aaccaaagca cagagaatcc caatgtagaa ggaattaacc tgttcaacct
     2641 tataggtctg ggcaataatc cctccaataa tcaagacaat cccaaataca gtatttttcg
     2701 aaatcttgtg cttcaaaaag aagaaagcca atagaactga aatcgcaggg taaatagccg
     2761 atacagatga agctaaagaa cttccgatat acttaactgc ataaagattg gcctgcatac
     2821 cgatagggcc tgctagcaag gctccgatga taacactgac attgcgaata tttaagaaaa
     2881 ttgagaggcg aactttccct tcttttacca agagaaaagc tagtaagata aagatgctca
     2941 aaaaatcatg agctgcagcc accacaaagg gtgacaaatc tgtaaaaatc gaaaagatat
     3001 aagcactaac cgttagaccc aagccccaga aaatacctga gagaaggcca aaaggaactc
     3061 cgtttttact tttcatctga acctccataa gaagccaaac ctttaacagc tctttggtaa
     3121 cgattcacac catagtcacc aaaatctgca ccttgctctt ccttatagac agtccataga
     3181 ctccaaatag tatcttgtaa aattttataa atagcaatct tttcatgaga aaccggtgtt
     3241 tggtcactct catagtgaga taagaaagtt tcttcctctt gggaagtgaa ttcagactct
     3301 aaaaagaggg cagccaaatc ccacattgga tcattcattg atgaatattc ccagtcaatc
     3361 aaataaagtc gtccttgagg tgattcgata aagttttcag gcaccaaatc gatatgacaa
     3421 gattttctgt caacacctaa gtcagccagt cttttctcta aggagaagac tgcatttcta
     3481 acagattcat agttggcata aggaatttgt tcttcaatca aggattcgta ttttttgatt
     3541 tcttcaaaag gagcaaattc tcctcttaat tccttagcag acgtatgaat agtttgtaat
     3601 attggagcaa ttttgtcgaa cttggtcttg attgacgttg aatcaagcgt aatcgcagat
     3661 tcgatatact catttacttt gataccagct tcaatatcaa aaagataatt ttttacatct
     3721 aagcctaaat cctttagtag ttcaagattg tacttttcat cttgtcgatt gataagcttt
     3781 tctgtccctt taccaaagaa tttaacaatg tattgcttat ttgttgtttt ggccaaatag
     3841 ttttgattgg tcattccacc cagttgttca acactgagga cttcctcttc ttgactaagt
     3901 aaggaagaaa ttttttcttt aatgattttc tccacaatta acctccagta cttatacttc
     3961 ccacttaaac actgttttaa aggctgtgtt taaatcggtt gcaaagacac gatgaatatc
     4021 tttaatttct cttacaggtt cttctagata aaggatattt ttaagacgat tggcaaattt
     4081 cttgacttcc atcatttgga tagcattttt aaaatcaatg cgaccagaac gagatgaccc
     4141 aaccaaaatc aagccctttt ctaaggcatc gcgagtattg agattgactt tatattcgct
     4201 aactcccatc atgagaatcg ttccctgagg acgaatgtag cgaatcaagt cattaatagc
     4261 tggtccagta ccatcaccac cacaacattc aaaagcatgg tcaaaggcca actcttcagg
     4321 aatattatcc gtaatatagc attctttggc aaatgagaag agttccaact tttcccaatg
     4381 acgaccaata accacaatct ctgattctgg caaagtatag ttgataatat tggcaaccac
     4441 aaaagctaaa cttccatctc caataacggc gatccgctcc cgcttgctat gagcaagagt
     4501 caatagacga ttcatagcgt gcatgcccac actgacaaac tctgtaatgg ctgcaaccgt
     4561 atcttcaata gcatcataag ccaccacacg atctttaggg agagaaacaa actctctcat
     4621 aaagccatca aatccactag acaagaaatg ggtccctgtc atgtagtttt catagaattc
     4681 ttcatcactc tgcataggag actgattggg aatcatgaca actttttgac caacctcgta
     4741 ggttccggtc gggtcagaaa tgacggttcc acatgactcg tgaatcattg ccattggaag
     4801 ctttttattc aaaatcttgg gatcacgttt tccctgatag taacgctgat ccgcatgaca
     4861 gacagccatg tagttgggac ggataaggat atgattctct tggtcaatag cctcttcctg
     4921 atatttgaca ttgataaact taggcttagt tagttgataa atttgattaa tcattttact
     4981 agtctttctc aatcatactt tttgcaatct tcaaatctgt tacggttgta atcttcagat
     5041 ttgagtattc acctttggcc aaggccacat cttttccttt gatcacaaag attttacatg
     5101 catctgtcaa gatttccttc tcttcatcag aaagagatcc ataaaggtcc atgaagtcct
     5161 tgcaacggaa tgtttgaggt gtttgtccct gatagaggtg agcacgattt ggaatacctg
     5221 taatgaattg cccattggta ctttcaacga tagtatccac tgcttctacc actgtgtcca
     5281 ctgcgtcatg attttgggca agttggatat tgtcctgaat catgcgaagt gtaataaatg
     5341 gacgaacaga atcgtgggta acaacgatat cctctgaagt aagcggacga taagcatcaa
     5401 tggcttcaat gatgttctca atacttgtat tgcggtcagc accacccttt gtaatgatga
     5461 tacgttcctt ataaagagga agatatttat ctacaagatc ttctgcatga gaaacccagt
     5521 ctccatgaac accaactaca attttttcaa tacttggctc caagacaaat ttttcaattg
     5581 tatgaatcaa aataggtcga tcacctagct ctaaaaattg ttttggcaag ttactgatcc
     5641 ccatgcgtgt gccagttcca ccggcaagaa ttcctgcata aatcatctat tttctccttt
     5701 gtcttactaa aaaaacttat tctctctatt ataccacaat ctagtcctat cataaaagaa
     5761 atactggtcc tccccttcta gaataggaga attatgcagt tctattattc aaagaaacta
     5821 gtccatttct agtaatgact aggaattctg tagcaattac taataaatga ctgcgaaatt
     5881 ttagagtcct tcagaataat atactttcct taaagaaaac ttaaaattta agatttgaaa
     5941 cttcaaagtg attgcttggc ttactgccct tcatttctta ttagctaact ttatgatata
     6001 atgaaaaacg aggtaaattg aatgaaaagt ataaaattaa atgctctatc ttacatggga
     6061 attcgtgtct tgaatattat ttttcccatc ctaactggaa cctatgtcgc gcgtgtcttg
     6121 gaccgaactg actatggtta cttcaactca gtcgacacta ttttgtcatt tttcttgccc
     6181 tttgcaactt atggtgtcta tagctacggt ttaagggcta tcagtaatgt caaggataac
     6241 aaaaaagatc ttaacagaac cttttctagt cttttttatt tgtgcatcgc ttgtacgatt
     6301 ttgaccactg ctgtctatat cctagcctat cctctcttct ttactgataa tccaatcgtc
     6361 aaaaaggtct accttgttat ggggattcaa ctcattgccc agattttttc aatcgaatgg
     6421 gtcaatgaag ctctggaaaa ttacagtttt ctcttttaca aaactgcctt catccgtatc
     6481 ctgatgctgg tctctatttt cttatttgtt aaaaatgaac acgatattgt tgtctataca
     6541 cttgtgatga gtttatcgac gctgattaac tacctgatta gttatttttg gattaaaaga
     6601 gacatcaaac ttgttaagat tcacctaagt gattttaaac cactctttct ccctctgaca
     6661 gccatgttag tctttgccaa tgccaatatg ctcttcactt ttttagatcg cctcttcctc
     6721 gttaaaacag ggattgatgt caacgttagt tactatacca tagctcagcg aattgtgacc
     6781 gttatagctg gggttgtaac aggtgcaatt ggagtgagtg tgcctcgtct cagttactat
     6841 ctggggaaag gagacaaaga agcctatgtt tctctggtta atagaggtag tcgaatcttt
     6901 aacttcttta tcattccact gagttttgga ctcatggttt taggaccaaa tgccatccta
     6961 ctttacggta gtgaaaaata tatcggaggc ggcatcttga cctctctctt cgcttttcgt
     7021 acgattattc tggccttaga taccattctt ggttcccaaa ttctctttac caatggctat
     7081 gaaaaacgta tcacagtcta tacagtcttt gctgggctac tcaatttggg cttgaatagt
     7141 ctcctctttt tcaaccatat cgtggctcct gaatactact tactgacaac tatgttatca
     7201 gagacttctc tacttgtttt ctatatcatt ttcatccata gaaaacaact catccacttg
     7261 ggacatatct ttagctatac tgttcgatac tctctctttt cactttcctt tgtagcaatt
     7321 tatttcctga ttaatttcat gtatcctgtg gatatggtca ttaatttgcc atttttgatt
     7381 aatactggtt tgattgtctt gctatcagct atctcttata ttagtctact tgtcttcaca
     7441 aaagatagca ttttctatga atttttaaac catgtcctag ccttaaaaaa taaatttaaa
     7501 aaatcatagg agtttaaaat gaaacaacta accgttgaag atgccaaaca aattgaatta
     7561 gaaattttgg attatattga tactctctgt aaaaagcaca atatcaacta tattattaac
     7621 tacggtactc tgattggggc ggttcgacat gagggcttta tcccttggga cgacgatatt
     7681 gatctgtcca tgcctagaga agactaccaa cgatttatta acatttttca aaaggaaaaa
     7741 agcaagtata agctcctatc cttagaaact gataagaact actttaacaa ctttatcaag
     7801 ataaccgaca gtacgactaa aattattgat actcgaaata caaaaaccta tgagtctggt
     7861 atctttatcg atattttccc tatagatcgc tttgatgatc ctaaggtcat tgatacttgt
     7921 tataaactgg aaagcttcaa actgctgtct ttcagtaaac ataaaaatat tgtctataag
     7981 gatagccttt taaaagattg gatacgaaca gccttttggt tgctccttcg accggtttct
     8041 cctcgttatt ttgcaaataa aatcgagaaa gaaattcaaa aatatagtcg tgaaaatggg
     8101 cagtatatgg cttttatccc ttcaaaattt aaggaaaagg aagtcttccc aagtggtacc
     8161 tttgataaaa caatcaattt accctttgag aatttaagcc ttcctgcacc tgaaaaattt
     8221 gatactattt tgacacaatt ttatggagat tatatgaccc taccaccaga agaaaaacgc
     8281 ttctacagtc atgaatttca cgcttataaa ttggaggatt aggatgcaat atttagaaaa
     8341 aaaagaaatt aaagaaattc aactagccct gctggactat attgatgaga cttgtaagaa
     8401 acatgatatt ccttattttc tcagttatgg aaccatgctt ggagccatcc gccacaaagg
     8461 tatgattcct tgggatgatg atattgatat ttccctttat cgtgaggatt atgagcgttt
     8521 actgaagatt attgaagaag aaaatcaccc tcgctacaag gttctttcct acgatacatc
     8581 ttcttggtac ttccataatt tcgcatcgat tttggacact tctactgtta tagaagacca
     8641 tgttaagtac aagcgtcatg ataccagcct tttcatcgat gtcttcccaa ttgatcgatt
     8701 tacagacttg agcattgtcg acaagagcta taagtatgtg gctcttcgtc aactagctta
     8761 tatcaaaaaa tcacgagcag ttcacggtga tagcaaacta aaagattttc ttagattatg
     8821 tagctggtac gctctccgat ttgtcaatcc tcgctacttt tacaagaaaa ttgatcaact
     8881 agtcaaaaat gctgtaacca acactcctca atatgaagga ggagttggga tcggtaagga
     8941 agggatgaaa gaaatcttcc cagttgatac ctttaaagaa ctgattttaa ctgagtttga
     9001 gggccgtatg ttgcctgttc ccaaaaaata tgaccaattt ttaacccaga tgtatggcga
     9061 ttatatgaca ccaccatcaa aagaaatgca agaatggtat agtcatagta ttaaagctta
     9121 tcgtaaaagc tgattgaggg ggattataca aactactaag atagaggtta ttcaaaaaca
     9181 taattttagt agaaaatgaa ata
//
LOCUS       DAVKJJ010000068         8901 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000068 DAVKJJ010000000
VERSION     DAVKJJ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8901
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.068"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            189..1976
                     /locus_tag="VW144_001829"
     CDS             189..1976
                     /locus_tag="VW144_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU9601240.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            1973..2449
                     /locus_tag="VW144_001830"
     CDS             1973..2449
                     /locus_tag="VW144_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9601241.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            2477..3382
                     /locus_tag="VW144_001831"
     CDS             2477..3382
                     /locus_tag="VW144_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9601242.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            3369..4184
                     /locus_tag="VW144_001832"
     CDS             3369..4184
                     /locus_tag="VW144_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9601243.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            4191..4595
                     /locus_tag="VW144_001833"
     CDS             4191..4595
                     /locus_tag="VW144_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9601244.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            4894..6060
                     /locus_tag="VW144_001834"
     CDS             4894..6060
                     /locus_tag="VW144_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU9601245.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESLATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTTLLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            6176..7213
                     /locus_tag="VW144_001835"
     CDS             6176..7213
                     /locus_tag="VW144_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEU9601246.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHVYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            7330..8543
                     /locus_tag="VW144_001836"
                     /pseudo
     CDS             7330..8543
                     /locus_tag="VW144_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein C"
BASE COUNT         2524 a         1666 c         2013 g         2698 t
ORIGIN      
        1 tcctatattc cgaatttaaa gagcgtttga aatgggtcta aaaatgtttg aaactaattg
       61 aatgtgtgta taatagtaaa aatgcagtaa atatcccgac aatttctatt gacaattcaa
      121 acagattggt ttataattaa tataacaaca aatgaaagcg caaactttcg cggtcggaag
      181 gtagttttat gacacgattt gagatacgag atgatttcta tctcgatgga aaatcattta
      241 agattttatc tggtgccatt cattatttta gggttcctcc agaggattgg tatcattcgc
      301 tctataactt gaaggctctt ggttttaata cggtagagac ttatgttgct tggaatttac
      361 acgagcctcg tgaaggtgag tttcattttg aaggtgatct ggatttagag aaatttctcc
      421 aaatagcgca ggatttgggt ctctacgcaa ttgtgcgtcc gtctccattt atctgtgcgg
      481 aatgggaatt cggtggctta ccagcttggc tcttgaccaa gaacatgcga attcgctcat
      541 ccgacccagc atatatcgag gcagttggtc gctactatga tcagttattg ccaagactgg
      601 tgcctcgttt gttggacaat ggtggcaata ttctcatgat gcaggttgaa aatgagtatg
      661 gttcttacgg agaagataag gcttacctga gagcgattcg acagctaatg gaagagtgtg
      721 gcgtaacctg tcccctcttt acatcagatg gtccatggcg agctactctg aaagctggaa
      781 ccttaattga agaggacctc tttgtaacag gaaactttgg ttctaaggca ccttacaact
      841 tttcgcagat gcaggaattc tttgatgaac atggtaagaa atggccactc atgtgtatgg
      901 agttctggga tggttggttc aatcgctgga aagaaccgat tatcacacgg gatcctaagg
      961 aattggcaga tgcagttcga gaggttttgg aacaaggctc tatcaatctt tacatgttcc
     1021 acggtggtac aaactttggt ttcatgaatg gttgctcagc tcgaggaact ttggacctgc
     1081 cacaagttac gtcttatgat tacgatgccc ttctggatga agaaggaaat ccaactgcta
     1141 aatatcttgc agtcaagaag atgatggcaa cacatttttc agagtatccg cagttggaac
     1201 cactctacaa agagagtatg gagttggatg ctattccact agttgaaaaa gtttctttgt
     1261 ttgaaacctt agatagcttg tcaagtcctg tagaaagtct ctatcctcaa aagatggagg
     1321 agctgggaca aagttatggc tacctacttt atcgaacaga aacaaactgg gatgcagaag
     1381 aagaaagact tcgtatcatt gatggtcgag atagggccca gctgtatgtc gatggtcagt
     1441 gggttaaaac tcaatatcag acagagattg gggaagatat tttttatcaa ggtaaaaaga
     1501 aagggctatc taggttagat atcttgatag aaaatatggg gcgtgtcaac tatgggcata
     1561 agttcttagc ggatacgcaa cgtaagggaa ttcggacagg ggtctgtaag gatctgcatt
     1621 tcttactaaa ctggaaacac tatccactcc cactagacaa tcctgagaaa attgattttt
     1681 caaaaggatg gactcaagga caaccagcct tttacgctta tgactttaca gtcgaagagc
     1741 caaaagatac ttacctagac ttgtctgagt ttggtaaggg ggttgccttt gtcaatgggc
     1801 agaatctagg acgtttttgg aacgttggcc caactctctc actttatatc cctcatagct
     1861 atctcaagga aggtgccaac cgcatcatta tctttgaaac agaaggtcaa tataaagaag
     1921 agattcattt aactcgtaaa cctacactaa aacatataaa gggggaaaac ttatgacaat
     1981 tgtaggatgc cgtattgatg gacgtttgat ccacggacaa gtagccaatc tttgggctgg
     2041 aaaactaaat gtttcacgca ttatggttgt agacgacgaa gttgtcaaca acgatattga
     2101 aaagagtggt ttgaaacttg cgacaccacc aggtgtgaaa ttgagtattt tgccagttga
     2161 gaaagctgca gccaatattc ttgctggcaa atacgatagc caacgtctct ttatcgtggc
     2221 tcgtaaacca gaccgcttcc ttggtttggt agaagcaggt gtaccacttg aaacccttaa
     2281 tgttgggaat atgtctcaaa caccagaaac tcgttctatt acacgttcta tcaacgtagt
     2341 agacaaggat gtggaagact tccacaaact ggcagaaaaa ggtgttaaac ttactgctca
     2401 gatggttcca aatgatccaa tttcagactt tttgagctta ttaaaatagg aaaaaaattt
     2461 ttaggaggtc attgttatga tacaatggtg gcaaatttta cttctcactt tgtactcagc
     2521 ttatcaaatc tgtgatgagt tgacgatcgt ttcatctgca ggttcccctg tatttgctgg
     2581 tttcattact ggtttaatca tgggagatgt gactactggt ttacttatcg gtggtaactt
     2641 gcaactgttc gttcttgggg ttggtacctt cggtggtgct tctcgtatcg acgcaacttc
     2701 tggtgcggtt cttgcgacag ccttctctgt ttcacaagga attgatgcac cgcttgccat
     2761 tactacaatc gctgtaccag tagcagctct cttgacttac ttcgacgttc ttggtcgtat
     2821 gactactacc ttcttcgctc accgtgtgga tgctgcaatc gaacgctttg actataaagg
     2881 tattgaacgc aactacttgc ttggtgcgat tccgtgggct ctatctcgtg cccttccagt
     2941 cttctttgcc cttgcttttg gtggtgcctt tgtacaatca gtagtagact tcgttgaagc
     3001 ctacaaatgg gttgcagatg gcttgacact tgcaggacgt atgcttccag gtcttggatt
     3061 tgcaatcttg cttcgttacc ttccagttaa acgtaacctt cactaccttg ctatgggatt
     3121 tggtttgaca gctatgttga ctgttcttta ctcatatgta acaggtcttg gtggcgctgt
     3181 tgctggtatc gtaggtactc ttcctgctga agttgctgaa aaaattggtt tcgtgaacaa
     3241 cttcaaaggt ttgtctatga ttggtatttc tatcgtaggt attttccttg cagtgcttca
     3301 cttcaaaaat agccaaaaag tagctgtagc agcaccttct acaccatcag aaagtgggga
     3361 aatcgaagat gacgaattct aattacaaac ttacaaaaga agattttaat caaatcaaca
     3421 aacgtagctt gtttactttc caattaggtt ggaactacga acgtatgcaa gcttctggtt
     3481 acctttacat gatcttgcct cagttgcgta aaatgtatgg tgatggaact cctgaattga
     3541 aagaaatgat gaaagttcat actcaattct tcaatacttc accattcttc cataccatta
     3601 tcgctggttt tgaccttgcc atggaagaaa aagatggtgt aggttcaaaa gacgccgtta
     3661 acggtatcaa gacaggtttg atgggaccat tcgctcctct tggggataca atctttggtt
     3721 cacttgtacc tgctatcatg gggtcagtcg cagcaactat ggctatcgct ggccaacctt
     3781 gggggatctt cctttggatt gcagttgcag tagcgtatga catcttccgt tggaaacagt
     3841 tggaatttgc ttacaaagaa ggggttaacc ttatcaacaa catgcaaagt accttgacag
     3901 ctttgattga cgctgcatct gtacttggtg tcttcatgat gggtgctctt gtagcaacag
     3961 tgattaactt tgaaatttct tacaagttgc caatcggtga aaagatgatt gatttccaag
     4021 acatcttgaa ccaaatcttc ccacgtttgc ttccagcaat ctttactgcc tttatcttct
     4081 ggttgcttgg taagaaaggt atgaactcta ctaaagctat cggtattatt atcgtacttg
     4141 ctttggctct ttctgccctt ggtcactttg cacttggaat gtaattcctt atgactaaat
     4201 cattaatttt ggtgagccat ggtcgcttct gtgaggagct tagaggtagc acagaaatga
     4261 ttatgggccc acaagacaac atttacacag tagctcttct tccagaagat ggcccagaag
     4321 aatttactgc taaatttgaa gctgttattg aaggattgga tgatttccta gtctttgcgg
     4381 atcttctcgg tgggacacct tgtaatgtgg tgagtcgctt gatcatggaa ggtcgtgata
     4441 ttgaccttta cgcagggatg aatcttccaa tggtgattga atttatcaat gcgagcctta
     4501 caggcgcaga tgcggactac aagagccgtg ctgcagaaag cattgtgaaa gttaatgacc
     4561 tgttagcggg cttcgatgat gacgaagatg aataatactc ttcgaaaatc tcttcaaact
     4621 acgtcaacgt cgccttgccg taggtatatg ttactgactt cgtcagtctt atccggcaac
     4681 ctcaaaacgg tgttttgagc tgacttcgtc agtcttatcc ggcaacctca aagcagtgct
     4741 ttgagcagcc tgcggctagt ttcctacaga ttttagttgg aactcgattc aattcatgtg
     4801 acaacgtgaa aatcgttaga gcattttata tagaatatac atgggaatgt agcttactcc
     4861 cattcccata tttaatagaa aaagaggaac tcaatgctac attatacaaa agaagacttg
     4921 ctcgaattgg gtgcagaaat cactacgcgt gaaatctacc aacagcctga tgtatggaga
     4981 gaagcttttg aattttatca agcaaaacgt gaagaaattg cagccttcct acaagaaatc
     5041 gctgataaac atgactatat taaggttatc ttgacaggtg ctgggacttc tgcttatgtg
     5101 ggagatacct tgctacctta ttttaaggaa gtctatgacg aacgcaaatg gaatttcaat
     5161 gctattgcga caacagatat cgttgccaat ccagcaacct atttgaaaaa agatgtggca
     5221 actgtccttg tgtcttttgc tcgtagtggg aattcgcctg aaagtttggc gactgttgat
     5281 ttggccaaat ccttggtgga tgagctttat caagtgacga ttacttgtgc agcagatggt
     5341 aaattggctc ttcaagctca cggtgatgat cgtaatctct tgctcttgca accagctgtc
     5401 tctaatgatg ctggatttgc catgacttct agctttacgt ctatgatgtt gacaactctc
     5461 ttggtctttg atcctacaga atttgctgtt aagtctgaac gttttgaagt tgtatctagt
     5521 cttgcccgta aagttttaga caaggcagaa gatgtcaaag agctcgttga tttagacttt
     5581 aaccgtgtca tctatctagg cgctggtcct ttctttggac ttgctcatga agctcagctc
     5641 aagattttgg aattaactgc tggtcaagtt gcgaccatgt atgaaagccc agttggcttc
     5701 cgtcacggtc caaaatctct tatcaacgac aatacagttg ttttggtctt tggtacaacg
     5761 acagactaca ctcgtaagta cgacttggac ttggttcgtg aagttgctgg tgaccagatt
     5821 gctcgtcgtg ttgtgctttt gagtgatcaa gcttttggtc ttgaaaatgt caaagaagtg
     5881 gcccttggtt gtggcggtgt cttgaatgat atttaccgtg tcttccctta catcgtttat
     5941 gcccaactct ttgctttatt gacttcactc aaggtagaaa ataaaccaga tacaccgtct
     6001 cctacaggta cagtaaaccg tgtagtacaa ggtgtcataa ttcacgaata tcaaaagtaa
     6061 gacagtgttt atgaattctt gacaagagga tttgtaaatt atcagataaa ccatagattg
     6121 tcagtacgct ttctatggtt tgtttgcttg agagaaatag taaaaggaga acagaatgaa
     6181 agcatacaca gagcgtgtat ttggaaatgt tgagggtgag gatgtcttgg cctatcgatt
     6241 tgagacagac ggtggctacc aacttgaggt tatgacttat ggtgcgacta tcttgcgcta
     6301 tgtcgcacct gacaaggctg gaaattttgc caatgttatc ttgggatttg atgactttga
     6361 tagttatgta ggcaatagtc ccaagcatgg agcaagtgta ggtcctgtag cgggtcgtat
     6421 tgcaggtgcg acctttgagc tcaatggtaa gacctatgac cttgaggtta ataatgctag
     6481 caactgtaat cacagtggtt caactggttg ggattccagc ttgtttgaag ttgaagaagt
     6541 aagcgatcat ggcttgactc tctacacaga gcgtacagat gggacaggag ggttccctgg
     6601 aaatctcaag atttggatca gttatcactt ggaagaaact ggtgcctatg aaatcagcta
     6661 caaggtaacg accgatcagg atacgctggt caatccaacc aaccacagct atttcaactt
     6721 gtctggtgat ttcacgcaga cgattgaccg tcatgtcttc caactaaaca cagagggcat
     6781 ttactcaatc gctcctgacg gtgttcctgc caaaactcca gaagccaacc gtgatgtggt
     6841 caaacacgtc tacaatggta ccttgttgaa ggatatcttt gcagaagaag atgagcaaat
     6901 ccagctggca tcaggtttgg atcatccatt tgcccttcct gcaggccatg acaatgctgg
     6961 attcctttat gaccaaaatt caggtcgctt cctgcttttc aagacagaag ctccttgctt
     7021 tgtggtctac acagcaaact ttgtggatga aagtgtcatc ataggaggtc agccaatgct
     7081 acagcacaat gggattgctc ttgaagcgca agctttacca gatgccattc acagtgacct
     7141 taaaggccaa gtcattctta aagctggtca aaccttcacc agtaagacac gttatgaact
     7201 tgttgtgaag taaaagagtc attgcgccta cttttgggag ctaggaatag gtacgcagag
     7261 acaaatagta ggaaaatatg atataactaa gcgttgaaag ctatctgtta atataatatt
     7321 caaactacaa taaggagtaa gaaagaaacg aagaaaattg tatttgctag tgccttggct
     7381 ttgaccttgg ctggagcagt tttgacaaat gatgtttttg cgaacgacag acttgtggca
     7441 acacaaacta ctgatggtaa aaatgaaaat gtattgacct cagaggtgct aaaaccttct
     7501 agtggcaatg ttttggttgg aatcaaagga gaatttgtgg ctcctcatca acaatctatt
     7561 ttggatgcca tcaatgctat ctgtaaagaa gcggctgacg aaggtttggt agataagtat
     7621 gtccctatca aatggtcaac tgacctagaa aaggcagctt ttgccagagc tacagaagca
     7681 tctataacca tggatcatac ccgtctttct agcaaagatc tttggagtgc ctttccaact
     7741 tctaatagta taatgggaga aaatttggca tggaatcatg acggttttct aaaagctatt
     7801 gaacaatggc gtgctgaaaa agcagattat gtggagaaaa aaatagtggt tcagacaacg
     7861 ggaaatctgg tcactatgag tcgctaatta accctaaatt tacacacatg gggatggcag
     7921 cttttaaaaa tcctaacaat caatacaaag ctattacaat tgctcaaact ctaggtgatg
     7981 atgcttcttc agaggaattg gctggtagat atggttctgc tgttcagtgt acagaagtga
     8041 ctgcctcaaa cctttcaaca gttaaaacta aagctacggt tgtagaaaaa ccactgaaag
     8101 attttagagc gtctacgtct gatcagtctg gttgggtgga atctaatggt aaatggtatt
     8161 tctatgagtc tggtgatgtg aagacaggtt gggtgaaaac agatggtaaa tggtactatt
     8221 tgaatgactt aggtgtcatg cagactggat ttgtaaaatt ttctggtagc tggtattact
     8281 tgagcaattc aggtgctatg tttacaggct ggggaacaga tggtagcaga tggttctact
     8341 ttgacggctc aggagctatg aagacaggct ggtacaagga aaatggcact tggtattacc
     8401 ttgacgaagc aggtatcatg aagacaggtt ggtttaaagt cggaccacac tggtactatg
     8461 cctacggttc aggagctttg gctgtgagca caacaacacc agatggttac cgtgtaaatg
     8521 gtaatggtga atgggtaaac taggctcagg ccataggtaa agcattcatc ttacttagca
     8581 aaaagaatga acgataagaa agaggttgat ggcgaacatt ggcctctttt gatttataaa
     8641 gattggattc ttgtcgcctc aatttcagac ttttctattg taagctaata ttttatagcc
     8701 cattaaaagc ataagcggta atctaattta aaaaatgctg taattagtct gaagtccaca
     8761 cttacttgtt gagatgttat ctctgttttt tatcgttata atttactgta ttttttatag
     8821 tatgcagaat atttttaagt atatttcaat agaaatttct atcgatttat tgtataatga
     8881 taagtaattg ttgaaaagta c
//
LOCUS       DAVKJJ010000069         8900 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000069 DAVKJJ010000000
VERSION     DAVKJJ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8900
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.069"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            129..1622
                     /locus_tag="VW144_001837"
     CDS             129..1622
                     /locus_tag="VW144_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9601247.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            1635..2177
                     /locus_tag="VW144_001838"
     CDS             1635..2177
                     /locus_tag="VW144_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9601248.1"
                     /translation="MKKVKLGEVCEILSGYAFKSSQFNDNKIGLPLIRIRDVERGFSD
                     TYFEGTYPEEYLIKNGDLLITMDGSFILKKWEGDLALLNQRVCKIKITDKSVDEGYIS
                     WLIPKFLKEIEDKTPFVTVKHLSVAKIKDISFVLPNKLEQKLIAKKLNTISQIYDFRK
                     IQSEKFNELVKSRYSEEVVA"
     gene            2174..2761
                     /locus_tag="VW144_001839"
     CDS             2174..2761
                     /locus_tag="VW144_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9601249.1"
                     /translation="MKKVKLGQVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSKGIN
                     YYSGTIDKKYIVEAGDILISWSGTLGVFQWCGRSAVLNQHIFKVVFDKIDIDKSYFKY
                     VVEKGLQDAVKHTHGSTMKHLTKKYFDNIMVSYTNLGEQQRIASELDLLSKLILRRQE
                     QLEELNLLVKSQLAIQKSLEELETLKKSLMQEYFG"
     gene            2821..3057
                     /locus_tag="VW144_001840"
     CDS             2821..3057
                     /locus_tag="VW144_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEU9601250.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            3054..3467
                     /locus_tag="VW144_001841"
     CDS             3054..3467
                     /locus_tag="VW144_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEU9601251.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            3585..4550
                     /locus_tag="VW144_001842"
     CDS             3585..4550
                     /locus_tag="VW144_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9601252.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            4573..5154
                     /locus_tag="VW144_001843"
     CDS             4573..5154
                     /locus_tag="VW144_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9601253.1"
                     /translation="MFGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLNTK
                     NVTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKHLRI
                     NSGMVILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQSQLPITKLKKILLPLPPLALQ
                     NEFADFVVQVDKSQFACEIAIKVWRNSLKFSII"
     gene            5184..8534
                     /locus_tag="VW144_001844"
     CDS             5184..8534
                     /locus_tag="VW144_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9601254.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYCATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIEQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISDQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFIFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
BASE COUNT         3045 a         1341 c         1789 g         2725 t
ORIGIN      
        1 gtttgaagag attttcgaag agtataaaat attcaggtga cgcatagata tagttaattg
       61 aagctttgtt tgaaatctga taaaataatg atattactaa gttttaaaaa ctaaagaaaa
      121 gggaagatat gattacaggc gaattaaaaa ataaaatcga ccagctgtgg gaaattcttt
      181 ggacagaagg aaacgcaaat cctttaacaa atattgaaca gttgacttat ctcttattta
      241 tgaaagattt ggatagtgtc gagcttggac gtgaaagtga tgctgaattt ctagggattc
      301 cttatgaggg agtttttcca aaagataaac ctgaataccg ttggtcaact tttaaaaata
      361 taggagatgc tcaggaagtt tatcgtttaa tgactcagga gatttttccg tttattaaaa
      421 atctcaaggg ggatacagat gatacagcct tttcacgata tatgcgagaa gctatttttc
      481 aaataaataa acctgctacg cttcaaaagg caatttctat cttagatgtt tttccaacta
      541 ggggattaga tgtagatttt gataatgaca aacaaagtat tactgatatc ggagatatct
      601 atgaatatct gttatcaaaa ttgtcgaccg caggtaaaaa tggacagttc cgtacacctc
      661 gtcacatcat cgatatgatg gttgagttga tgcaaccgac tatcaaagat atcatctcag
      721 atcccgctat gggttctgct ggcttcttag tatctgctag ccgttactta aagcgtaaga
      781 aagatgaatg ggaaaccaat acagataata tcaatcattt tcataatcag atgtttcatg
      841 gaaatgatac ggatacgact atgttgagac ttggggcgat gaacatgatg ctacatggag
      901 tagaaaatcc acaaatcagt taccttgact cgctgtctca agataatgaa gaagccgata
      961 aatatacttt ggttttagca aatcctcctt ttaagggctc acttgactac aattcaacct
     1021 ctaatgacct tcttgcaacc gtaaaaacca aaaaaacaga attactcttt ctttctcttt
     1081 tcttgcgaac tttaaaacca ggtggacgag cagcagttat cgtacctgat ggtgtccttt
     1141 ttggttcgtc taaagctcat aaaggaattc gtcaggaaat tgtagagaat cataagcttg
     1201 atgctgtaat ctcaatgcct agtggtgtgt tcaagcctta tgctggagtt tcaactgcca
     1261 ttctcatctt tacaaaaact ggtaatggtg gtactgacaa agtctggttt tacgatatga
     1321 aagcggatgg tttaagtttg gatgataagc gacaaccgat tagcgacaat gatattccag
     1381 atattatcga acgctttcat catcttgaaa aagaagcaga acgtcagaga acggatcaat
     1441 ctttctttgt tccagttgct gagataaagg aaaatgatta tgatttgtct atcaataaat
     1501 ataaagagat tgagtatgaa aaagttgagt atgaaccaac agaagtcata ttaaagaaaa
     1561 tcaatgattt agaaaaagaa attcaagctg gcttggctga attggaaaaa ttactcaagt
     1621 agggaggtgg ctgtatgaaa aaagtgaagt tgggggaagt ttgtgagatt ttatctggtt
     1681 acgcttttaa atcatcacaa tttaatgata acaaaattgg tctcccctta atacgaattc
     1741 gagatgtaga aagaggtttt tctgatacct actttgaagg aacttatccc gaagaatatc
     1801 ttattaaaaa tggagactta ttgattacaa tggatggcag ttttatattg aaaaaatggg
     1861 agggggattt agcgctactt aatcaacgtg tctgtaaaat taaaattacg gataaaagtg
     1921 tagatgaagg ttatatatct tggttgattc ctaaattttt aaaagaaatt gaagataaga
     1981 ccccttttgt tacagtgaaa catctttcag tagctaaaat aaaagatatc tcatttgtat
     2041 tgcctaataa actagaacaa aagctaattg caaaaaaact aaatacgata agtcaaattt
     2101 atgattttcg taaaattcaa tcagaaaaat ttaacgagct cgtcaaatcc cgatattccg
     2161 aggaggtggt tgcatgaaga aagtgaaatt agggcaagtg gctactttta tcaatggcta
     2221 tgcttttaaa cctcaagatt ggtcatctga agggaaagag attatcagaa tacagaatct
     2281 gactaaaact tcaaaaggga taaattatta ttcgggtacg attgataaaa aatacattgt
     2341 tgaagctggt gatattctta tttcgtggtc gggtacatta ggggtttttc agtggtgtgg
     2401 aagatccgca gtgttaaatc agcacatttt taaagtggtt tttgataaga tagatataga
     2461 taaatcgtat tttaaatatg ttgttgaaaa aggtttacaa gatgctgtaa aacatactca
     2521 tggttccaca atgaaacatt taactaaaaa atattttgat aatattatgg tttcatatac
     2581 aaatttggga gagcaacaaa gaattgcaag tgagctggat ttgttaagca agctgatttt
     2641 aaggagacaa gaacaacttg aagagcttaa tctacttgta aaatcacaat tggcaatcca
     2701 aaaatctctg gaagaacttg aaactttgaa gaaatctctg atgcaggagt attttggctg
     2761 atattctgcc attgtaatta cggtaatgat ttgttataat acttcaaagg aggaaatcag
     2821 atggtagtaa aaacaagaaa acaaggaaat tcaatcacca ttacgattcc aagtgaattt
     2881 aatattccaa gtggtgttaa atacgaagcg aaattgttac caagtggtga gattatcttt
     2941 actcctgaag aattggggca gcaggtttct tatgtatctg atgatgcctt tgacttaaat
     3001 ttagataaaa tatttgacga atacgacgat gttttcaaag ctttggtgga aaaatgacaa
     3061 tctatctgac agaaaagcaa attgaaaaaa taaatgcttt agcaattcaa cggtattctc
     3121 caaatgagaa aattcaaaca gttagttctt ctgccttaaa tatgattgtg aacttaccag
     3181 aacaatttgt ctttgggaag tctctttatc caacaatttt tgataaagca acgatactat
     3241 ttgtccaatt gataaagaag catgtttttg ctaatgctaa taaaagaact gctttcttcg
     3301 ttttggtcaa atttttacaa ttaaacggct atcgtttttc tgtaacggta gaagaagcag
     3361 taaaaatgtg tgtaaccatc gcagtagaag ctttaactga tgaaaaaatg acaagctact
     3421 ccaaatgggt ttctgaacat tctgttagag aaaaggtcaa aaagtaacct agtatgctgg
     3481 atttgaatga gcacaagaaa ataaatgaac agacaatatt agaattctgt aatgcagaaa
     3541 ctgatattgt ctctttttat tgatgaataa gaaagtgaga aattatggaa tcaaaagtta
     3601 caattatcat gcaagaaatg ttacctcttt taaataatga acaattacta gcgttgagag
     3661 agagtttaga acatcatcta gtagacggaa aaaagcagca gaagtattcg aataataacc
     3721 tgttgcaact atttattacc gccaagcagg tagagggctg tagctcaaaa acaattcgtt
     3781 attaccagag gacgattgaa aacttgttta atgctattaa agagtctgtg acacaactca
     3841 caacagatga tttaaggagt tatttagcaa attaccagtc tgaaaaggat tgtagtaagg
     3901 caaatttaga caatattagg cgtatattgt cttctttttt tgcttggctt gagcaagagg
     3961 aatatatcat taaaaatccc attcgacgga taaagaaaat taagactgag caaaatgtga
     4021 aggaaactta tactgatgaa catttggaaa ttatgcgtga taactgtgaa aatttgagag
     4081 atttggcaat aatagaccta ctagcatcga caggtatgcg tgtaggggag cttgtacagt
     4141 tgaatcgttc agatattgat tttgaaaaca gagagtgtgt tgtctttggt aaaggaaaga
     4201 aggagagacc agtatatttt gatgctcgga cgaaaattca tttaagaaat tatcttaacg
     4261 acagaaaaga tagtcaccct gctctttttg taacgctagt tggaaaagcc cagagacttg
     4321 gaattgctgg tgtagagatt cgcttaagaa agttaggaga caaactcggc atacaaaagg
     4381 ttcacccaca taagttcaga agaactttag cgactaaggc aattgataaa ggtatgccta
     4441 tcgaacaagt ccaaaaactg ctaggtcata gcaagattga cacaaccctg gcctatgcca
     4501 tggtcaatca aaataatgtc aagcattcac accaaaaatt catctcttaa aagcaaatcc
     4561 cgatttaacg agatgtttgg ggaaaataaa atatttgaaa gcattgataa cttatttgat
     4621 attatagatg gtgatagggg caaaaattat cctaaatcag atgagttgtt tagtgaggag
     4681 tactgtttat ttttaaatac aaagaatgtt actaaaaacg gattttcatt cgatacaaag
     4741 caatttatca ctaaaacaaa ggataaatta cttcgaaaag gcaaacttga gcgttatgat
     4801 atagtcttga caacaagagg tactgttgga aatgtagcgt actacgatga attaataaaa
     4861 tataaacatt tacgtataaa ttcaggtatg gtaatattac gtcccaagac accaaatcta
     4921 aatcagaaat ttattatcca tgttttaagg aataataatt atagtcgagt gatatcagga
     4981 agtgctcagt ctcagttacc aattacaaaa ttaaaaaaaa tacttctccc cctcccccca
     5041 ctagccctcc aaaatgagtt cgcagacttt gtagtccagg tcgacaaatc acaatttgct
     5101 tgtgagatag ctataaaagt gtggagaaat agcttgaaat ttagtataat atagctaaac
     5161 tatttgttta aagtgagaaa aaaatgggaa attttagctt tcttttaaaa aatgacgaat
     5221 atgaatcttt ttcaaaacct tgcattgaag ctgaaaatat gattgctaca tcaactgtgg
     5281 ctactgcctt tatggcgcgt cgtgctttgg agcaggctgt ccattggata tatagtcacg
     5341 attcatattt agaagctccc tattgtgcta ctctatcttc tttagtatgg gatgatgatt
     5401 ttagggatat cgtagattct gaactccaca agcagatagt tctgttgatt cggtggggaa
     5461 accatgctgc tcatggtggt gaaattaagg aacgagaagc gattttagct ttacatcatt
     5521 tgtatcagtt tgttaatttt atcgattatt gttacagcaa tgaatttgtg gagcgttatt
     5581 ttgatgagaa gtgtttacca ctttcagcaa acatcaaata ccgagaaact ccacaatcta
     5641 tgataaagtt acaagacagt ttaccagaac tgcctgattt tcatgaacag atggctgctc
     5701 agtccgtaga agttcaagag acttatactg aaaaacgtga gactgcagcg caacggcaag
     5761 atgtgccttt ccatattgaa caattatctg aggcagagac aagaaagctc tttattgata
     5821 tcgatctccg tttagcagga tggatatttg aagaaaactg tcgtgttgag atagccgttg
     5881 atggtctcaa gcacggttca ggaattggtt actgtgacta tgtactttat ggtaaaaatg
     5941 ggaaaatttt agcgattgtg gaggctaaaa aagcctctgt caatccagaa gtaggggaag
     6001 tacaggtcaa agaatatgct gaagctttgg agaaacatat cggctatcag ccaatttgct
     6061 ttattacaaa tgggttgaag cactatatac ttgatggtcc gaaccgccgc cagattgcag
     6121 gcttttactc tcaagaagaa ttgcaattag tgatggatag acgtcatctt caaaaaccgc
     6181 ttgaggatat ttctagtaaa attagggacg atatttccgg gcgtcactac caaaaacatg
     6241 ccattgcaag cgtttgtgaa gctttctcta atcatcgtag acaggcactt ttggttatgg
     6301 caactggggc ggggaaaact cgtacagcag tttctctagt tgatatctta tcacgtcata
     6361 actgggtaaa aaacgttctc ttcttagccg atagaacttc cttggttaag caagcctatg
     6421 attcgtttag aaaattactc ccagatcttt ccgtttgtaa cttcttagaa gataaagaag
     6481 gagctcaatc aagtcgtatg gtcttttcaa cttatccgac catgattgga gcgattagtg
     6541 atcaagaaga agtaaatcaa cgccctttta ctgttgggca ttttgacctt atcataattg
     6601 acgaatctca ccgttctatt tatcagaaat acaagtccat ttttgattat tttgatgcaa
     6661 ggattgtagg cttaacagct actccgcgtc aagatttaga taaaaacacc tatggattct
     6721 ttaatttgga gaatggggtt ccaacatatg catatgattt ggaagaggct gttaaagacg
     6781 gatatttagt agcctatcat tctatcgaaa ccaaactgaa actacctacg gatggtctac
     6841 attatgatga tttgtccgaa gaagaaaagg aacattttga tagcaaattt gaagacgata
     6901 gctgtgaaaa agatattgat gggagtgtgt ttaattcctt tattttcaat aaaagtacag
     6961 tagaaattgt tttaaatgaa ctcatgacaa gaggaattca gacagcctcg ggtgatgaaa
     7021 ttggtaaaac tattattttt gctaaaaatc atgaccatgc ggaatatatc agaggtattt
     7081 ttaacaaccg ctatcctgaa aaagggagcg actatgctca ggtgattgat tatagtatta
     7141 agcattatca gaccttgatt gatgatttta aaattaagga gaagtatcct caaattgcga
     7201 tttctgtcga tatgttagat acaggtattg atgtaccaga ggttgttaat ttagtcttct
     7261 tcaagaaagt acgctctaaa actaagtttt ggcagatgat tggtcgagga acccgtctat
     7321 gtaaagattt atttggacct gagcaggata aggaaaactt cttggtattt gattatgggg
     7381 acaattttga ttattttcgt gcagatccaa gagatggaga gggtcgtcac attgtttcgt
     7441 tgactcagcg tttatttaat atcaaagtgg acttgattcg agaacttcag ggacttcaat
     7501 accaagaaga tcagtttgcg agagcatacc gtcagcagct tgtctcggaa cttcaaggtc
     7561 gtatagagag cttaaatgag ttggacttca gggttcgtat ggttttagat acagtttata
     7621 gctataggaa attggaaagt tggcagaatc taactgctgt tacaagtgaa accattcaaa
     7681 aaaatctctc tccgctttta tttgatgaag ataaagaaga tgagatggcc aggagatttg
     7741 atttgtggtt gcttcatatt cagttggggc aactgacagc taaatcttcc actgttcata
     7801 tttcccaagt gatgaagacg gctagagctc tttctgctat tggcaatatc ccgcaggttt
     7861 ttgagcaggc tgaaattatc aggaaagtac aggagcctga attttggaaa gaagttaact
     7921 tgtctgattt ggaaaaaatt cgtcttgcta ttcgagattt attacagttt ttggataaaa
     7981 cagaccgtaa accctactat gttaactttg aagatcgtat actctccact gttcacgaga
     8041 ccacagcatt tttgcaggtc aacgatcttc ggtcttacaa tgaaaaagtt gagcattatt
     8101 tgaaaactca tctggatgag gagtccattt ctaagctata ccataataaa aagttgacat
     8161 ctgatgatat gcttgcactt gaaaaattgc tctgggaaaa attaggtagt aaagcagact
     8221 accaaagtca ttatgaaaat aaggcaattc cgagattggt tcgtgagatt attggcttag
     8281 atagagagtc tgccaatcgt attttttcta aatttttgtc ggatgagaat cttaatgcca
     8341 ggcagatttc atttgtaaaa ttgattgtag actacattgt agaaaatggt tttttagaga
     8401 cgaaagtgtt aacgcaagag ccgtttaaat cttatggttc tgttcaacta ctcttccaac
     8461 accaactacc agtacttcgt aatattgttc aaatcattga acttatcaat aatcgagctg
     8521 gagaagcggc ttaaattcta aagtgattgc catgctgaga ctcatttaaa attaaaaaga
     8581 gtagaaattt atgctatata tgagaagttt tattaggaag aatgtcatcg ttttcctaga
     8641 atacagtatc agttgttaag tggttgataa atttcaaagt agatacttgt accacgatgt
     8701 ttgttgatcg agttattcac gatgtttgtt gatcgagtta ttaacaaaag agctactttg
     8761 attttaaaga aatagaaaac aaaaagctga gcaagaattc aattgcagga gaaaatgaaa
     8821 taatactcaa tgaaaatcaa agagcaaact aggaaactag ctgcaggctg ctcaaaacac
     8881 tgttttgagg ttgtagataa
//
LOCUS       DAVKJJ010000070         8649 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000070 DAVKJJ010000000
VERSION     DAVKJJ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8649
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.070"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(778..1647)
                     /locus_tag="VW144_001845"
     CDS             complement(778..1647)
                     /locus_tag="VW144_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9601255.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIVA
                     AIAAVAVFKFLYL"
     gene            complement(1871..2392)
                     /locus_tag="VW144_001846"
     CDS             complement(1871..2392)
                     /locus_tag="VW144_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9601256.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVVSGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPDPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAS
                     LGLGFFKKKEDEK"
     gene            complement(2350..3456)
                     /locus_tag="VW144_001847"
     CDS             complement(2350..3456)
                     /locus_tag="VW144_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEU9601257.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(3567..3821)
                     /locus_tag="VW144_001848"
     CDS             complement(3567..3821)
                     /locus_tag="VW144_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601258.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(4161..4607)
                     /locus_tag="VW144_001849"
     CDS             complement(4161..4607)
                     /locus_tag="VW144_001849"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9601259.1"
                     /translation="MDETGIDRYLYRPYAGAPRGEKVYEKISGRRFERTSIVAGQVDG
                     EFIAPMIYKKSMTSDFFVEWFKTQLLPALKTPHVIVMGNASFHPKNILDELCIQDKHF
                     FLPLPPYSPDLNPIEQAWAILKKKVTDLLREVPTIFECLERFFKTR"
     gene            complement(4680..5005)
                     /locus_tag="VW144_001850"
                     /pseudo
     CDS             complement(4680..5005)
                     /locus_tag="VW144_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5239..6957
                     /locus_tag="VW144_001851"
     CDS             5239..6957
                     /locus_tag="VW144_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEU9601260.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMTKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKITNIEAE
                     INAFVK"
     gene            7068..7415
                     /locus_tag="VW144_001852"
     CDS             7068..7415
                     /locus_tag="VW144_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9601261.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFVGL"
     gene            complement(7466..8419)
                     /locus_tag="VW144_001853"
     CDS             complement(7466..8419)
                     /locus_tag="VW144_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HEU9601262.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRR
                     WLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG"
BASE COUNT         2495 a         1798 c         1570 g         2786 t
ORIGIN      
        1 taaaaattta actaattaat agaaagtact aaatagactt atccttcaag gagttttcag
       61 cattatccat ccactcactt gctgaaaatg taaacagtct ctggtttata tagtaaaatg
      121 aaacaagaat agagcaaatc gatcaggaca gtcaaatcga tttctaacaa tgttttagaa
      181 gtagatgtgt actattctag tttcaatcta ctatacaacc tcaaaactgt gttttgaaca
      241 acctgcggct agcttcttag tttgcccttt gattttcatt gagtataaaa ttgcagataa
      301 aacgataaca atcataggac tcagcagagt caatgtaggt cgaagaatac ccccctaatc
      361 gcttatttta tagcaaaatg aaatagaaac tgaacatttt gttgaggaag gtaaatcaat
      421 tcctcacaat gtttgagaaa gaagggagga ctattccaac ttcaacccac tataaagcag
      481 acttacaaga tagggaaccc atctccgata atatatagta gaatgaaaac tgcacaattt
      541 gattggagaa ttcaaaccaa tttctcacaa tgttttagaa acaagggtct attatttcaa
      601 ctgcaaccta ctatagtcca ttgaagctgg aatagcccac tgagacttct aaaccactgc
      661 tggagaggag tttgtttaga gtttctttca tttccctaaa aaatcaaaaa gagagaacca
      721 gtctgatcct ctcttattta tagatggtgc tcactcaaat gttaaaggag ctatttctta
      781 gagataaagg aatttgaata cagctactgc cgcaattgct gctacgatag gtgctactac
      841 tggtacccaa gaataccacc attttgaatc gcctttatgc tcaccaagaa ctgattttgg
      901 aaggaaagca tgaaggagac gtggtcccaa gtcacgggct gggttcaagg caggtcctgt
      961 aggtcctcca agtgatgtta ccaaagccat aacgaggaat ccaagtgcca agtgagccac
     1021 agaaagtcct gaagcagtgt gtggagccac ctgtgcttta atagccaagt cagaaaaatc
     1081 aactgtttgt cctgcttcta ttgccttttg tttcatgaat tgaagcactt cagcaccaaa
     1141 gaagttttta gtcaaaccaa gagctgcaaa gaacaaaaca aatgaaccaa caaactcatt
     1201 aatcaaacca ttgacagttg ctgcatagcg actttctttt gtaccatggt caatacttga
     1261 aatagttgag aaagttccca agatgttatt tgggttttca gttttcaagt agaatggacg
     1321 gtatgttgcc acaactaagg cttggccaaa gatagctccc aagacttgcg cgataatata
     1381 aggtaccact tgtgcccaag ggaaaagacc gctaactgca agccctagag tgaaagcagg
     1441 gttgatgtga ttcccagata cgttaccaaa catcaaggct gggatcataa cccccatacc
     1501 ataaccaaca gcgatgacga tccagccact ttggtgacct ttcgtacctt taagttcaac
     1561 gttggcaact gcaccattcc caagaatgat caaaatggca gttcccaaaa attcagtggc
     1621 atacttcagt gcccatgtga aatccatttg ctagattctc cttaaaattt tttagatatt
     1681 ccttatttta tcaattttta ccctatttag caactgattt ctggaaatat ttttaaaaaa
     1741 ataggattca tagtttccca gttcaacgga cttgtaagcg tttttattct tcacgaaaga
     1801 cctcgttctc aaaaccagga aaaaagccca catctgtgag cttttcctgg ttcctagatt
     1861 ctaaaatcta ttatttttca tcttcttttt tcttgaagaa tcctaatcct agactagcta
     1921 acaaggtcat gataccagca ctagccaagg tagcattagc ttctgtacct gtgtttggca
     1981 attcctctct cttacctgtc tcataagtcg gaacttctgg gtctggattc actggagttt
     2041 cagtttttgg agtacctggg tctggagttg gtttatctgg tgttgataaa cggtcatacc
     2101 ttaccgttat ttctttatca ctagagtctg acgtaacttc ttgtgattca actgttggaa
     2161 tatctggatc tttgtacttg tcaatcttac cagatacaac ctcgtcccag tttcctgttg
     2221 tccattcacc gtaggttaca actcccgtga ccttgttctc agtttttgta cggcttaagg
     2281 ttacaggttg aacaacatct tcttttacat tttggttcgt aactttatca acgtaatgaa
     2341 tgatacgcgt tatagtcttc gtctcagtag aggttgctgt tttgggaacc actgtttcct
     2401 caacattctc acggtagtaa tagtcaactg ttgcaccgtc ttctggtacg catttgcagg
     2461 agttgctacc aaggtgtatg ttgttttcct tgtgataact cggtcttctt tgtcctcagt
     2521 tgttgttttc ccttcaatag tttttgattc tgtggtatac tcagaaccta tcgctaaatc
     2581 agcttttata acagactctg ccaacttctc ttggctacct tctttatagt aattggctgt
     2641 tactgtagca gtggttggcg cttcgctttc tctataaact aaggtcactg ttctaccttc
     2701 gcttacaata ttcccagtta aacttgcaga atttgtatct gcttctttaa aagtataata
     2761 ttttccgtca gtagtagtca tgctactgag ttttttatct gtgacattgt agctggtacc
     2821 aatcagttgt tttttattgg taatgtaggt tccgtcactt tctttttctc caattccagt
     2881 atcatttttc aatgatagca aacgcccttg ttcatcaaca tagcgaactt tcacattttc
     2941 tgagattagt tctgccaatt ctgaggtttt tttctttttc ttgatttctt cggttatttt
     3001 ccctttctct tcttcgggaa tatttagttt tggaatgatt ttttcaacaa cggttcgtga
     3061 tggttccaca gtatcttgga tgactgaaaa gtcagctaga attgggagat tatactgaac
     3121 acggtgactt tgagtgttta ctcctactct ttcattattc tctgaaaata cttgtacggt
     3181 ataagaaaca acatcttttc ctaatagaac atccccagta gagaaatagc cgccttttcc
     3241 tagtttgcta tctccagagc ccacttcttt cctaatctta tcagatagtt ttttaccagt
     3301 cagtacattc gttcgcacaa tccctttgtc tacccctaca aagtgggaga actttttgaa
     3361 ctcttcagaa ccagatctag cccaaccatt attaagggca tttgcttttg tatttgtatt
     3421 ctctctcaaa agtttggcga ttagaattat attcatcggc acttagagtt gctgctatat
     3481 ctgactcttg aataccaact tccttactac catttctagc ggcagtatat gtgtatttat
     3541 ttgtttctac ttctagctga ttatccttaa catgataggt aattttctcc tggtataaga
     3601 gtcttcagta gtttatatgc tctgtcatca tctttagctt tagaagcagc tgagtttagc
     3661 caagttgggg tattaggagt atctttatca gcctgtttca agacattatt caagtcattt
     3721 gctagcctat tggttgtcct cactgtgaaa gttttcttcc agtctgaacc acttttatta
     3781 gacttagata tgctcatgaa atcctggtca acattcaaca caatatcgcc ttgtttttta
     3841 gcatcttccg ttgcttctgc ttcaaattga tcatggagtt tttgttgctc tggtgtatag
     3901 tttttttatt tacacgattt aatgtactat tttttatttt agtattaaga actccattaa
     3961 tacgattttt aaactctgct tcaaaaaatc ccttaatttg ttggtgcgca agttcaacat
     4021 tcaaaccttt cttatacaag tcttcattct gaacgatttc ggctacacgg tgaatggttt
     4081 gatcgtatac cgtcttctct taaccagcag tatccttagt tttaatgact actcgataag
     4141 ttcctttaga accgttatag tcatctagtt ttaaaaaagc gttccaaaca ttcaaaaata
     4201 gttggaactt cccttaataa atccgtcact ttctttttca agatagccca agcttgctca
     4261 ataggattca aatccggtga ataaggtggt agaggtaaga aaaagtgttt atcttggatg
     4321 cagagttcat ccaaaatgtt cttgggatga aaactagcat tgcccatgac aataacatga
     4381 ggtgtcttca aagcaggtag gagttgcgtt ttgaaccact ccacaaagaa atcgcttgtc
     4441 atgcttttct tgtaaatcat gggagctata aactctccgt ctacttgtcc tgcaacaatt
     4501 gaagttcgct caaaacgacg tccgctaatc ttttcataga ctttctcccc tctaggagcc
     4561 cctgcataag gacgatagag gtagcggtcg attcctgttt cgtcaatata aacaggtgct
     4621 aggtgcttta aactattaaa attcttaaga aataaggcta cttttctggg tcttgttcct
     4681 taaagctcgt catctttttt taaagtgacc ttaatctgct ttaaagctgc ccatactgaa
     4741 ggaacagcac aatcaaaatg tgccgcaatt tcccgtaaaa aagcatctgg atgagcctct
     4801 acaaaggctt tcaattcctc taaaggaatc tttcggtttt tgacgactcg ctttttcctc
     4861 tctatgtgtt cttgttcacg tctttctttt cccacgtgaa gagagttctg acgccaacac
     4921 caaaaaactt ggctgcctcg acatggctgt gcccctcttt gatgtaatct aatgctcgct
     4981 gtttaaaatc agtactatat gccatagctt tattataata tgcttgttta aaactaagta
     5041 actatatcaa tgttttgtta tgagaagact tacttttttt actgaaattg aatttttcct
     5101 agcctcaaca tcatataacc ccaaaattca atctgacttg gtttgaagag atgttcgaag
     5161 cagataaggt tttactaaaa ctaaaaaaaa tgatataatt aaactgatta aatgttttaa
     5221 aaataaagga gaatttgaat gtcttaccaa gaaaattacc agaaatgggt tgattttgtg
     5281 gagcttcctg actaccttcg tcaagatttg gaaaatatgg acgaaaaaac taaggaagat
     5341 gccttctata caaatcttga atttggtact gcaggtatgc gtggcttggt tggtgctggt
     5401 acaaaccgta tcaacatcta cgttgttcgc caagctactg aaggattggc tcgtttgatt
     5461 gagtcaaaag gtggaaacga gaaagaacgc ggtgtagcaa ttgcctacga tagccgtcac
     5521 ttctcacctg agtttgcctt tgaatctgcg gcagttcttg ctaaacacgg catcaaatct
     5581 tacgtatttg aaagccttcg tccgactcca gaactatcat ttgcagttcg tcatctcaac
     5641 tgtttcgcag gtatcatggt cacagccagc cacaaccctg ctccatttaa cggttacaag
     5701 gtttacggtg aagacggtgg acaaatgcct ccacacgatg cggacgcttt gactacttat
     5761 atccgtgcaa tcgaaaaccc atttgcagtt gaagttgctg atgtggaaac tgaaaaagct
     5821 tctggcttga ttgaagttat cggcgaagct gttgacatag aataccttaa agaggttaag
     5881 gacataaaca tcaacccagc cttgattgaa gaatttggta aagacatgaa gattgtctac
     5941 acaccacttc atggtactgg tgaaatgttg gctcgtcgtg ctcttgccca agcaggattt
     6001 gactctgttc aagttgttga agcgcaagca actgctgacc cagacttttc aactgtaaca
     6061 tctccaaacc cagaaagcca agcagccttt gcccttgctg aagaacttgg tcgtcaagtt
     6121 ggtgcagatg ttcttgtcgc aactgaccca gacgctgacc gtgttggtgt tgaagttctt
     6181 caaaaagatg gcagctacct caacctttca ggtaaccaaa tcggtgctat catggctaaa
     6241 tacatcttgg aagctcacaa aaacgctgga actcttcctg aaaatgccgc tctctgcaaa
     6301 tctatcgttt caactgactt ggtaacgaag attgctgaaa gctacggcgc aactatgttc
     6361 aacgtcttga caggtttcaa atttatcgct gaaaaaatcc aagaattcga agaaaaacac
     6421 aaccacactt acatgatggg atttgaagaa agcttcggtt acttgattaa accatttgta
     6481 cgtgataaag atgccatcca agccgttctt gtagttgctg aacttgctgc ctactaccgt
     6541 tctcgtggtt tgacacttgc tgacggtatc gaagaaatct ataaagaata tggctactac
     6601 gcagaaaaaa caatctctgt tactctttca ggtgtcgatg gtgctgaaca aatcaaagcg
     6661 attatgacta aattccgcaa caatgctcca acagaatgga acgcaacagc tatcactgtc
     6721 gtagaagact tcaaggcaca aactgctact gttgctgacg gtactgttac aaacttgaca
     6781 actcctccaa gtgatgtgtt gaaatacaca cttgctgacg gttcatggat tgccgttcgc
     6841 ccttcaggta cagaaccaaa aatcaagttc tacattgcag ttgtagggga aaccaacgaa
     6901 gaatcacaag ctaagattac taacatcgaa gcagaaatca atgcatttgt aaaataaaga
     6961 cctataaaag atgagaccaa ccaacctcat cttttttcgt atcaaatcta agcaatttta
     7021 gaaactttat ggcatactag acttatcaat gaaagcgagg taatctcatg gaacaatttt
     7081 tagataacat caaagacctt gaagtcacta cagttgtgcg tgcgcaagaa gctcttgata
     7141 aaaaagaaac tgcaaccttc tttatcggtc gcaaaacttg cccttactgc cgtaaatttg
     7201 caggtacatt gtcaggtgtc gtagctgaaa ccaaagctca catttacttc atcaatagtg
     7261 aagaaccaag ccaactcaat gatttgcaag cattccgctc acgttatgga atcccaactg
     7321 taccaggttt tgttcatatt acagatggac aaatcaacgt ccgttgcgac tcttcaatgt
     7381 cagcacaaga aatcaaagat ttcgtaggat tgtaaaaaat cagctaacaa gtcttaaaat
     7441 cacaaaccca aatttcaagt tgaagttagc cacccaaaag aaattcactt ggcgacttgt
     7501 agtccaagca ttttttaggg tagttgttaa tccaattttc gataaaagct acttctttcg
     7561 gagtcgtttt cttggttcct ttaggtaacc atctccgaat taatcgatta tgattttcat
     7621 ttgaacctct ctcccatgaa gagtaagcat gtgcgtagta gatatgttcc tcaggaaata
     7681 cctcagacaa tcgtttgaac tccgaaccat tatctgcagt aatggacaga atacgatgct
     7741 cccctaatag taacgtaagc gcctgattga cagattcagc agttttattt ggaattaacc
     7801 ttataatttg gtgtctgctc cgccggtcgg ttaagactaa caggcaataa ttttttatct
     7861 tagtcagtag gacggtatca atttcataat gaccattttc caagcgaaga ttaataacgt
     7921 caggacgttc ttcgatagat ttacctgccg gcttaaagtt cggactagct tgcttcttga
     7981 cacctttcct ttttctggga taaagcatgt cggccttcgt caatcctaaa tgaccattat
     8041 gaaaccagta gtagatggtt gaaataccaa ctttcacttg cttcttgtta accatcattt
     8101 caggcgaaaa tttttgctta tgatagtgta agatcttctc tctgatttcc tttgttaaaa
     8161 ttaacttttt caccgaccgt tttcgattga attggtaaac agtttgtgcg taatcggcag
     8221 aatagacctt tttgtatagc ccttttctca cttgttgtaa agttgtacct cttttgactt
     8281 cattatgaat cgtttgaggc gcctttccta acaagcctgc aatttcacga ttggacttat
     8341 tttcattctt ccaccgctca atcaagcgac ggttatctat tgtcaaatgt ttcccttttg
     8401 gggtataatg ttcttgcatc tctgtgcctt tccttgtgtt tgtggtgaac aacaagtata
     8461 gcacagaggt gttttcttat accttaaatc acatttcatt tgacaacagg aacccttgaa
     8521 cctgtttttt cagctaaacc aaggctatga ttagccttgg ccacctagcc taacagtttc
     8581 aaacctgtta tcaaatgaaa tcatcaactt actcaaacat agtgaaacat ctaattcttg
     8641 actaaggta
//
LOCUS       DAVKJJ010000071         8214 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000071 DAVKJJ010000000
VERSION     DAVKJJ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8214
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.071"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(12..770)
                     /locus_tag="VW144_001854"
     CDS             complement(12..770)
                     /locus_tag="VW144_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9601263.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            complement(767..1633)
                     /locus_tag="VW144_001855"
     CDS             complement(767..1633)
                     /locus_tag="VW144_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9601264.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMDLMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            complement(1702..2706)
                     /locus_tag="VW144_001856"
     CDS             complement(1702..2706)
                     /locus_tag="VW144_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9601265.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEVQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     misc_binding    complement(2819..3036)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3119..5815)
                     /gene="ppc"
                     /locus_tag="VW144_001857"
     CDS             complement(3119..5815)
                     /gene="ppc"
                     /locus_tag="VW144_001857"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEU9601266.1"
                     /translation="MSLQKLENYSNKSVVKEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWHNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFDSSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     gene            complement(5835..7064)
                     /locus_tag="VW144_001858"
     CDS             complement(5835..7064)
                     /locus_tag="VW144_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9601267.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSATSVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            complement(7220..7522)
                     /locus_tag="VW144_001859"
                     /pseudo
     CDS             complement(7220..7522)
                     /locus_tag="VW144_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(7970..8191)
                     /locus_tag="VW144_001860"
     CDS             complement(7970..8191)
                     /locus_tag="VW144_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9601268.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTDSAYAKIERGEHTLTADI
                     LVTLSNFYDVSTDYLLGLTDFPDKIRFRK"
BASE COUNT         2504 a         1741 c         1419 g         2550 t
ORIGIN      
        1 ttttccctac tttattcaaa gagttgataa taatcagaga ttttcatttt tgctttttct
       61 tcttggttta aatcttggat aatccgtcct tctttcatga caatcaagcg attgccgtat
      121 ttgagagcat cttccatatg atgagtaatc ataagggctg ttagctgatc tttcttaaca
      181 aattcatctg tcaattccat caaagcaaca ctagtctttg gatccagggc agcagtatgc
      241 tcatctaaca ggagtaattc aggtcgcttc aaggttgcca tcaagagact caaagcctgt
      301 ctttgtccac ctgataagaa ctcaatcggt gtattcaagt gtttctcaag accatttcct
      361 actttttcaa tggttgcctg aaattcatcc ttatagctag tcaagcgtct tggtaacaat
      421 ccacgctttt caccacgaaa cttggcgatt aaaagatttt cagcgaccgt catacgggga
      481 gctgtcccca tctttggatc ttggaagaca cgagacaggt acttggcacg cttctcgggt
      541 gaaaacttag tgagatcttc acctaaaata cggatagttc cactagttag tgataaggtc
      601 cctgctatag tgttaaagag agttgatttt ccagcaccat ttccgcccaa aatcgtgata
      661 aagtcccgtt caaaaatttc taaggaaaca tcatttaaaa taatcttttc ttcatcaaag
      721 ccatttttaa cgattttggt tgcatttttt aattctacaa ttgctgtcat ttgcttaact
      781 tggctccttt caagattgtt tgcttaaatg ttggaatcat gaggcagact gctaaaatca
      841 aagcactgta taaacgaagg taacttgtat taaagccaag tgcgataact gcccacacta
      901 aaaattgata agcgatagaa cctacaacga tagtaaccaa acgctctgcc aagctcaaac
      961 tcttgaaaat aacttctccg ataatcaaac ttgcaagccc cacaacgata accccgatcc
     1021 ctcgagacac atcggcatag ccttcttgct gagcaatgag ggcacctgca agggcaatca
     1081 caccatttga taagaccaag cccatgagat ccatgcgtcc agtatgaatc ccgaaacttc
     1141 tagccatatc aggattatcc cctgtagcaa tataggcttg tccgagttta gtgtccaaga
     1201 aaaagagcat gagagcaata acaatgctca caaagatgag acctgtcaag agttgattca
     1261 aatccgaatc aaaaggcaaa acatcctgaa tttgcttggt tccaagcagg cctaaatttg
     1321 cacgtcccat aatcaagagc atgattgagt gacaagaagt catcaccaaa atccctgaga
     1381 gcaaggttgg gatcttccct tttgtataaa gaaggcctgc tgccattcca gccaaacaac
     1441 ctgctcctac agcaacaagt gtcgctaaaa atggattcac gcctttggtt atcaaagtga
     1501 cagcaacagc tcccccaaga gggaaggaac cttctgtcgt catatctgga aagtttaaaa
     1561 tcctaaatgt cataaagatt cccagaccta gaatagccca gacaaatcct tgagaaataa
     1621 tggaaacaat catattttat ttaatccttt ctatattcat ctttttaaaa aatgggaaga
     1681 gtctcctcct ccctacctta tttattcgat gacttgtcct gcttctttga gaacagactc
     1741 aggaatagta atacctagtt cttgtgctat ttttttattg atgactgact taccagttga
     1801 aaagacattg actggggtat cggctggttt tgcacctttc aagacttgca caatcatttt
     1861 acctgttgcc acaccaaggt catgttggtc aattacaact gatgccaaac cacctacttc
     1921 taccatagct gtcgcactgg gataaattgg tttcttagaa ctttgattgc tagagacaac
     1981 cgttggaaat cctgatgcaa tggtgttatc aattggaacc caaatagcat ctaccttgct
     2041 agtcataaca gtgacagttg aggcaatttc atttgttgaa ggaactgcaa atgtttccac
     2101 tgtcagacct gctttttcag cataagcctt aaattcttcg acctgtgttt ttgaattgtc
     2161 ttcgctactt gagtaaagag ctccgattgt tttcacattc ggtgtcagag ccttgatgag
     2221 ttcaacttgt tgttgagctg gattgtggtc agatacccct gtaacgttgc cacctggttt
     2281 tttcaaatct ttaaccaagt tagcaccaat tgggtctgta atagcggcca tgataaccgg
     2341 taggtctttt gttgcactag ccaacccttg ggctgctggt gttgcgatac caaccacaag
     2401 gtcattccca tttgcaacca attgtttact cattgtcgca accttacttt ggtcaccttc
     2461 tgagttcata aaatcaattt taacttgatc atctttatat ccttcttctg caagtccatc
     2521 ttggatccct ttataaatca aatcaaggga tggatggctc acaaattgaa gcacaccaat
     2581 cttggttatt ttcttctcat ccttattatt ctgaacttct gatttattca ttgaagaata
     2641 aatcaagctt cctgctacta agactgctaa tgcagcaata attccaatta aacgtttatt
     2701 tttcattctg tttctccttt ttatttcctt taacatattt cacttatcta aacaagcgac
     2761 gccattgttt tctttccata ctaacctcca tcaaaaaaat cctcacacaa aaaacttgtg
     2821 tgaggacgtc gatgcgcggt accacctcaa ttatagggaa tttccctatc gctcagtctc
     2881 gcaataacga gatgcactgt aaggtgtgct taccgaattt ttataatttc aaattctaaa
     2941 tagcattcag cccaattttc atcattgcca cttatctgtt ttcagctacc acagactctc
     3001 taaaaagttt gtatgattac ttttctgaat ggttaaatta tatcattttt aaactacttg
     3061 tcaatagtct attagaattt ttttgatatt cacttttagc attcactctt gaaaattatc
     3121 aacctgaatt acgcaatcct gtcgcaattc cgttgatggt gatatgaatc aatcgttctt
     3181 gatcactgga caattctcca cgacgttggc gtttaatcaa ctctaactga atatagttga
     3241 gaatattaaa gtaaggcata cggtaatcca gactagcttt tagatatgga ttgtcagcta
     3301 agagttcgtc atgtccttca atagccaaga taacgttctt agtaacttgc cattcattta
     3361 aaatagtctc atagatggcc ttaacttgct cgtcttcaca aagtttagca tattcaaaag
     3421 caatattcat atttgatttt gacaaaacca tatcaacatt tgaaagaagc gattggaaga
     3481 aaggccaatt ttggtacata tctcgtaaga tagcaatatt ctctggattt ttattgataa
     3541 attccttgaa gcttgaacca accccgtacc atccagggaa cataacacga ctctgtgacc
     3601 atgagaatac ccaagggatg gcacgcaatc caccgatttc agtaatagtc ttacgagcgg
     3661 ctggacgaga accaatatta aaacttgaaa tagccttgat tggacttgaa tcgaagaaat
     3721 aatcatagaa atgctcatta ccaaagacca aatcacggta gatatcgtaa ctacggtcca
     3781 ctacttgatc cataatggct tcataacgat ttggggtatt ggtatcgctc ttcttctgag
     3841 taatcatacg gttaatagct gccgatacta gcatttcaag gttatagtaa gcggcgtctt
     3901 tgttaccgta tttattccca attacttcac cctgctccgt caagcggata cgatccttga
     3961 tagacttgag cggttgagat gtaatggctt cataggttgg cccaccacca cgaccgacag
     4021 taccaccacg accatggaag aaggtaacct taacgccaaa ttcatctcca atagcagtca
     4081 attgttgttg agccttgtag agggtccaac atgatgacaa gtaaccgcca tctttattac
     4141 tgtcagagta gccaagcatg atttcttggt agttatttcg tgagtcaatc cattttttgg
     4201 caaggctaag agaaagatat tttctcattg tttcctctga atgatccaag tcttcaattg
     4261 tttcaaaaag gggaacaatc tgaacacgcg ccctttccgt atccaccagt cctacttctt
     4321 ttaacagaat agctaattct agcatatcag aaaggctggt tgcatgtgaa atgatggtct
     4381 gacggatgac atcatctccc aacttatctt tcaaaacacg agccgtctta aaaatagcta
     4441 attcttttgc taataattct gatttttctg cgtgagtcgc agaaagaatt cggggatctt
     4501 cttctaattc tttcaagaga aggtcacact tttcttcttc gctcaactcg ctataacgag
     4561 aatgaattcc tgctgatttc aagagttctg ccacacaggc ttcatagacg ctagagtctt
     4621 gtcgcatatc aattgacgct aagtaaaaac caaatatctc tactgcctgc aataattcca
     4681 caaaatcacc tgaaatcaag gactcaccct tattttctag tagagaatct cgaatggcaa
     4741 tcaaatcctt gtagaaatca ttggcggttt cataacgagt cccaacttct tcatcctcaa
     4801 tcagataggt tttagttgcc tgaatttttg attgaatatc aaacaaggca cgacggtaaa
     4861 gctctttttc gcggtaaata gagttatcct tggattgacg agccatttct ctgacttgct
     4921 tgctgacgtt gacaatgcta gttgaaagag aaaattcacg ataaagttgg taaatctttt
     4981 tatcatagta gttcatgatg acttcacact gagtgagtgc agactgcttc aaggtctttg
     5041 ctgtaacaaa tggatttcca tcacggtcac cacctatcca catacccatg gtgattggtt
     5101 tagcctgttt taaattcaga ccatgcgctt gcgctaagcg cttatactcc gtcgtcaaat
     5161 gagggacagc tttcaaaaag gagctgttgt aatattccat agcattcgtg atttcgttag
     5221 tcacttttaa ttttttctca cgaatcatgt ctgtctgcat gataatttcg atgtaacgac
     5281 gcaaatcatt gtgccattta tctttattga ttaaccccaa cttaacatca cggtatttac
     5341 gcaaaagact atgaatatga tttgttaaat ccaacatact tttgcgttgc acttgtgttg
     5401 gatgggctgt caaaacaggg acaacattca agtgttctag gatctcaacg gcattttcct
     5461 tttctgctac caatttaatc gttgtagata atttacctaa atagtcctga tcaatattat
     5521 tttgatgatt gatttcataa gctaaatcca catcctctga aatattaatc aaaagaggta
     5581 agatagagaa atagcgtgaa atatagacca tttcatcatt tgataagcta gtcactagac
     5641 ggtttagacc ttgataatct tcctgcgttg ataattcttt caactgtatt attttttcaa
     5701 aggtctctgg ggcaagcata tttttagtaa tatcttccag taattctgtt agaatcaaga
     5761 cttcttcttt cacaacactt ttattactat aattttctaa tttttgaaga gacataaact
     5821 atcctttctt tatcctactt caacagaagg ttcattggtt gattttccaa ttctcggtac
     5881 aacttagcgc gtttttcact ggcatcaata tttaagacaa aggctactgc cactgataag
     5941 actagaagac tatttccacc ctgggataag aaggggaaag tcactcctgt agatggaatc
     6001 aagcccgaaa tccctccgat attgacaaat acctgaacca acatcatccc tccgacaccg
     6061 agtgcaacca tggcattgaa aggattctcc gctcggatac cgaccaagat aatccgcaaa
     6121 atcatgaaaa acaagagagc taaaataaga ctggcaccaa caaagccaaa ttcttcaatc
     6181 acgatagaaa agacaaagtc tgtatgagct tctggcaaat aacctcgttt ttcaatcgag
     6241 tttccaagac ctagaccaaa ccaaccgcca ttgaccatgg caaaataaga attagctaac
     6301 tggtgacctg catcagcacg atcggcaaaa ggattaaaaa aggcactaaa gcgcttggct
     6361 acatagccaa atactggaat ttttgaaaag gtctcaacac cgattaggct gatagtggtc
     6421 aagacaaaga cagaagtggc agatacgagc gccagaatgg ttgaaaacca gcgataagcg
     6481 attccactaa ctgtatacat aatcaaggaa accaagacta aaatagtcgc atttcctaaa
     6541 tcagggaaaa ttcccaaact tccaatcaga actaggagaa cgaatcgcca atcattaaaa
     6601 gcacggggaa gccattgatt ttgagtcaaa acttgaaaat cataagtagc tatttcttct
     6661 tgctgtttgg agaatcggtg agctaaatac caaataataa tgatttttaa gtactcagct
     6721 ggctgaatag ttactcctgc aaccgaaatc caaccgtatg ccccgtttac ggaaatacca
     6781 ataaaacgag ccaagaacaa taaaagcatt tctattaata taactaaaat gattagtcgc
     6841 tcatttctca aaaaatctag tctcaattta taaattaagg caatcagtat caaactaaca
     6901 atccaaaaga ttccttggtt tcgaaccaac tgcaaggcac tcttgccttc ttcaattaaa
     6961 atagcactgg tggtcgaata gaccacaatc aagcccaaaa tagataaaag caagtaggga
     7021 atcaagatgg aataatttaa taagtgcctc ttactaatct tcatagcatc accactctac
     7081 taggatacag ataaatctgt tccaaattcc gttttatttt ctagttttga ttgctattat
     7141 accatttttt cagaaagaaa aaaactctta ccttacaatc cttcgaattt tactgttttt
     7201 acagaattaa ggcagaattt gaaatcgtaa aaccattttg aataagagtt gcttcaaccc
     7261 attgactccg acggattaag ttgctttcgt gaatgccaaa atcagccgca atttgttcat
     7321 aagtgctcta ttctcgcatg tattgaatag ttaccataag gagatcgtct aggattaatt
     7381 tgctttttcg tccacctttt gcgcgtttac gttgataagc tgtttttaac acagctaaca
     7441 tctcttcaaa agtcgtgcgc tgaacaccta caaggatctt gaaacggcta tcgcttaatt
     7501 gtttacttgc ttcatcatcc atagttttat tgtattgtat ttttgtttcg caggaagtct
     7561 attattcctt tcgttccaag gtttttacgc tttttcaagc aatcctacac actccacgtg
     7621 atgcgtctgt ggaactcaaa aggttcgggg aaccttttga ggattaattg cgtttctcta
     7681 acaaactcac acattcaacg tggcatgact gatgaagtca tcataagaat ttcacttgct
     7741 gctctttctc ggtggcagcc tgaacggtca gaagtatcat tgtatatcat ttgtatcatg
     7801 gcatataaag tatcgctcta ttttattgat ggttttaata tcgctcttat catggcgaac
     7861 cattcaaatc tgctccacaa atgaggaaag taccattttg gactattcaa ttgtcaaaga
     7921 acaaataaaa aaaatcttcg cattttcaca ctctatgaaa ttgaggagat tattttctaa
     7981 agcgaatttt atcaggaaaa tccgtcaatc ccaataggta gtctgtactg acatcgtaaa
     8041 agtttgagag agttaccaat atatctgctg ttaacgtatg ctcaccccgt tcaattttag
     8101 cataagctga gtctgtaaac gaaagtattt tagctatttg tttttgggtc aaatcgtgat
     8161 cttccctcaa atctctcaaa cgtctgtaca tctagctttc tcctgagaaa agta
//
LOCUS       DAVKJJ010000072         7721 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000072 DAVKJJ010000000
VERSION     DAVKJJ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.072"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            63..605
                     /locus_tag="VW144_001861"
     CDS             63..605
                     /locus_tag="VW144_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEU9601269.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            complement(646..719)
                     /locus_tag="VW144_001862"
     tRNA            complement(646..719)
                     /locus_tag="VW144_001862"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(683..685),aa:Asn,seq:gtt)
     gene            complement(725..796)
                     /locus_tag="VW144_001863"
     tRNA            complement(725..796)
                     /locus_tag="VW144_001863"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(761..763),aa:Glu,seq:ttc)
     gene            complement(848..1600)
                     /locus_tag="VW144_001864"
     CDS             complement(848..1600)
                     /locus_tag="VW144_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9601270.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            complement(1597..2922)
                     /locus_tag="VW144_001865"
     CDS             complement(1597..2922)
                     /locus_tag="VW144_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9601271.1"
                     /translation="MDLFGFGTVIVHLLIISHNYHLICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIAIFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDKDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            complement(2943..3068)
                     /gene="comC"
                     /locus_tag="VW144_001866"
     CDS             complement(2943..3068)
                     /gene="comC"
                     /locus_tag="VW144_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEU9601272.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            complement(3234..3307)
                     /locus_tag="VW144_001867"
     tRNA            complement(3234..3307)
                     /locus_tag="VW144_001867"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3271..3273),aa:Arg,seq:tct)
     gene            complement(3351..3830)
                     /gene="rlmH"
                     /locus_tag="VW144_001868"
     CDS             complement(3351..3830)
                     /gene="rlmH"
                     /locus_tag="VW144_001868"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU9601273.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            4013..5194
                     /locus_tag="VW144_001869"
     CDS             4013..5194
                     /locus_tag="VW144_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU9601274.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLRSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            5252..6010
                     /locus_tag="VW144_001870"
     CDS             5252..6010
                     /locus_tag="VW144_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU9601275.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            6223..7584
                     /gene="dnaA"
                     /locus_tag="VW144_001871"
     CDS             6223..7584
                     /gene="dnaA"
                     /locus_tag="VW144_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU9601276.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
BASE COUNT         2685 a         1389 c         1213 g         2434 t
ORIGIN      
        1 aaattacaag ataaaaaatc tatttttaga catatagtct aatttgttta caaggaggaa
       61 atatgcaaga aagtaacaaa cgcttaaaaa caaagcgaac tattgaaaat gctatggtac
      121 aattactgat ggaacagcca tttgataaaa tttctactgt caaattagta gaaaaagccg
      181 gaattagtcg ttccagcttc tatactcact ataaggataa gtatgatatg attgagcact
      241 atcaaagcaa gctattccat acatttgaat atatttttca aaaacatgct catcacaaaa
      301 gagacgctat tttagaagtt tttgaatatc tagagtcaga accacttctg gctgcccttc
      361 tttctgaaaa tgggactaaa gaaatccaaa atttcttacg aaataaactt catatcatgc
      421 ttagtacaga tttacaaaag cgatttatgc aactgaatct aaataccact gaattagaat
      481 atagtagcat ctatctaact catgcacttt ttggtgtctg ccaaacttgg attgcacatg
      541 gaaaaaaaga aagttctcaa gaaataacag acttccttat gaaaatgctt ggtgatacaa
      601 attgatacaa aaagaggaac acagctgtgt tcccttttat ctatactccg ccagtaggac
      661 tcgaacctac gacatcatga ttaacagtca tgcgctacta ccaactgagc tatggcggat
      721 aaaatagtcc gtacgggatt cgaacccgtg ttaccgccgt gaaaaggcgg tgtcttaacc
      781 ccttgaccaa cggaccttct atctgtagca gatataacca ttatatcaat ttcttgctaa
      841 ttgtcaatca cttttgagat tttttctcta aaatatcttt taattttcta atttttaatc
      901 ttgaaatagg acaacgatgg tcttcataga aaacaatttc taagtttttt cgatcaattt
      961 ctctgatatt gcctatattt accaaaaatg acttgtgagg agaataaaat cgctgagtat
     1021 gtttgtcctt ttcctgaata tctgtcatgg taccataaaa ctcttttgca aaattcttac
     1081 caataatgcg caatttatga gatacccctg ttgtttcaat atacaaaata tcatggtaag
     1141 gaatttttaa atcatttccc ttgtaattgt agtcgaaata atctacaaca tcttcatttt
     1201 caagtaacat actcttcgtg tagaagatat tttgctcaat tctcttctta aacatctcat
     1261 cattgatatc cttatcaaca aaatctaggg ctgatacctg gtatttatag gttagagtcg
     1321 caaactctga tcgactagtg ataaagacga taatagcgta aggattgtaa tgacgaatga
     1381 gctgagccac ttcaaatccc tttttctcaa ttccatgaat atcgatatct aggaaataaa
     1441 gctgatttac ttcatcattt tcaatgtatt cttcaaattc acggactttt cccgttgtct
     1501 tgtatgatat tggaatattc gattctttcg aaatttcatc caatattctc tctagtctca
     1561 cttgatgttc aataacatct tctaaaatta aaactttcat tcaaattccc tcttaaatct
     1621 aatgatttgt ctaaatgtac tgccttccat ctctgtttct aaaataatat tgttgtactt
     1681 atctagtagt tctttcacat tatttaatcc gactccgcga tttcttccct tagtggagaa
     1741 tcctaaggca aatagatctc ctgaaggagt catcgtcatt ttacatgaat tctgaatcac
     1801 aataactgtt tcagtttcca tcttaataac tgctacttcc atctgctttt tatagctatc
     1861 agccgatcct tcgacagcat tattcaataa aacgctcatg atacgaacca aatccaatag
     1921 ttcaattgga agcttggtaa tcgtatcttt tacttccagt gtaaactcta caccattatt
     1981 tcgagcatag acaattgact gagcaaccaa acttcgtaaa gctgagtctt ctatgttgtt
     2041 caaatcaaag taagtgtact tatctgaacg caatttatga tttgctttga ctaaaacttc
     2101 attgtaaatt ctgtcaattt cctgtaaatt accactgtca attgccatct gcatgctgac
     2161 aagcattcca gcataatcat gtcgaaaacc acggatttca ttatacagac caacaatttc
     2221 atctgtgtaa ttctgtaaat gtttctgttc aaatttcttc tgcttcaaag caatctcttt
     2281 ctccatttga actttatgag aattcattgc aaagaaggtc aaaaggagag agataaagac
     2341 aatagatgac aaaatacttc caaaactatt caaatgttta atcgtactta ccatatctga
     2401 aacgaaagat acaatatgta gcaatagtaa agcaaaaaat acttttttca agaaaggata
     2461 aaggtagtcc ttgtcaaaat aggctagttc caaatggaaa taataaatga tttttaatgt
     2521 aacaaaatag gttaacaccg tcacaacgaa aaagaatggg aaatgatatt gtaaaacaaa
     2581 attatctcct gttatagagg agaaagttac ggacagaaag ttatgagtgc tctcatataa
     2641 aagagatagt agtaaactta ggaatagtcc tctatccctc tcatactgtt tcatccatcg
     2701 aaaataggaa tataatccca aaggaaataa aaatattgca atccctaacc catctaaata
     2761 tagaatatat aagggaaaat caaatactat ttctgttagt aatgtataag caccaaaaac
     2821 gaataattct tttctattta tttgaccttt acaaattaaa tggtagttgt gactaataat
     2881 taaaagatga acaataactg tcccaaatcc aaataaatcc attactcttt ccccttattt
     2941 cattactttt ttctttgtaa aataaaatca cggaagaatt ttgacagcct catctcccca
     3001 cctttaatct tttgtaagtc tttttccttc aaagctacaa actgttccaa tttaactgtg
     3061 tttttcataa taaaatctcc taaaatgttt ttttcttgta agctaactta caaaaaccat
     3121 tatacaaaat ggaatttcgt tttagataaa attctctcaa ctgtcatttt tttctcccaa
     3181 agtgtacttt tttaagaaaa aagccgggaa aattcccagc tttgctatta tattgatccc
     3241 agcaggattc gaacctgcga ccgttcgctt agaaggcgaa tgctctatcc agctgagcta
     3301 tgagacctaa cacaattatt ctaccaaaaa ttcaattaaa agtcaatttt ctatttatgg
     3361 taggggaatc cctgctgaat cgtaaaagcg cgatagattt gttcaacaag aactagtctc
     3421 attaactgat gaggtaaggt taggcgacca aaactgacag aaagattggc tctatttttt
     3481 acagatgatg acaatcctaa acttccccca ataataaaag taagagtaga aaatcctttt
     3541 atagaagttt cttctaactg cttactaaat tcttctgaga agaaagtttt cccttcaatg
     3601 gctaacacaa taacgaaatc acggtcagca atttttgata aaattctctg accttctatt
     3661 tctaaaatct tttgattttc tgattcactg gccttatctg gtgttttttc atctgataac
     3721 tcaatcattt caaacttagc aaatctagaa attcgttttg aatactctgc gataccatct
     3781 tttaaatact tttctttcag tttcccaact gttacaactt taattttcat gactctattc
     3841 taacatattc tctatttttt cacatcttat tcacaaaata aaaaatagat ttcaattaag
     3901 aaaatcacag tttcaaaaga gttatccaca gtttgtgtaa aacttttgtg tttaagttat
     3961 aattaagcta gtcagtttat actttcagta attcaaacat atggaggcaa atatgaaaca
     4021 tctaaaaaca ttttacaaaa aatggtttca attattagtc gttatcgtca ttagcttttt
     4081 tagtggagcc ttgggtagtt tttcaataac tcaactaact caaaaaagta gtgtaaacaa
     4141 ctctaacaac aatagtacta ttacacaaac tgcctataag aacgaaaatt caacaacaca
     4201 ggctgttaac aaagtaaaag atgctgttgt ttctgttatt acttattcgg caaacagaca
     4261 aaatagcgta tttggcaatg atgatactga cacagattct cagcgaatct ctagtgaagg
     4321 atctggagtt atttataaaa agaatgataa agaagcttac atcgtcacca acaatcacgt
     4381 tataaatggc gccagcaaag tagatattcg attgtcagat gggactaaag tacctggaga
     4441 aattgtcgga gctgacactt tctctgatat tgctgtcgtc aaaatctctt cagaaaaagt
     4501 gacaacagta gctgagtttg gtgattctag taagttaact gtaggagaaa ctgctattgc
     4561 catcggtagc ccgttaggtt ctgaatatgc aaatactgtc actcaaggta tcgtatccag
     4621 tctcaataga aatgtatcct taagatcgga agatggacaa gctatttcta caaaagccat
     4681 ccaaactgat actgctatta acccaggtaa ctctggcggc ccactgatca atattcaagg
     4741 gcaggttatc ggaattacct caagtaaaat tgctacaaat ggaggaacat ctgtagaagg
     4801 tcttggtttc gcaattcctg caaatgatgc tatcaatatt attgaacagt tagaaaaaaa
     4861 cggaaaagtg actcgtccag ctttgggaat ccagatggtt aatttatcta atgtgagtac
     4921 aagcgacatc agaagactca atattccaag taatgttaca tctggtgtag ttgttcgttc
     4981 ggtacaaagt aatatgcctg ccaatggtca ccttgaaaaa tacgatgtaa ttacaaaagt
     5041 agatgacaaa gagattgctt catcaacaga tttacaaagt gctctttaca accattctat
     5101 cggagacacc attaagataa cctactatcg taacgggaaa gaagaaacta cctctatcaa
     5161 acttaacaag agttcaggtg atttagaatc ttaattgaca tctatgtaaa gaaagcttta
     5221 cataagagaa aagatgtgtt agtgtagaat catggaaaaa tttgaaatga tttctatcac
     5281 agatatacaa aaaaatccct atcaaccccg aaaagaattt gatagagaaa aactagatga
     5341 actagcacag tctatcaaag aaaatggggt cattcaaccg attattgttc gtcaatctcc
     5401 tgttattggt tatgaaatcc ttgcaggaga gagacgctat cgggcttcac ttttagctgg
     5461 tctacggtct atcccagctg ttgttaaaca gatttcagac caagagatga tggtccagtc
     5521 cattattgaa aatttacaga gagaaaattt aaacccaata gaagaagcac gcgcctatga
     5581 atctctcgta gagaaaggat tcacccatgc tgaaattgca gataagatgg gcaagtctcg
     5641 tccatatatc agcaactcca ttcgtttact ttccttgcca gaacagattc tctcagaagt
     5701 agaaaatggc aaactatcac aagcccatgc gcgttcccta gttgggttaa ataaggaaca
     5761 acaagactat ttctttcaac ggattataga agaagatatt tctgtaagga agttagaagc
     5821 tcttctgaca gagaaaaaac aaaagaaaca gcaaaaaact aatcatttca tacaaaatga
     5881 agaaaaacag ttaagaaaac tactcggatt agatgtagaa attaaactat ctaaaaaaga
     5941 cagtggaaaa atcattattt ctttttcaaa tcaagaagaa tatagtagaa ttatcaacag
     6001 cctgaaataa ggctgttctt ttattttttt atctcacaag gttatccact atgtttttcg
     6061 ataaaaatct taataaatca ataatttctt cttttatccc caacctgtgg ataaagttta
     6121 gtaacattgt ggattatttt tcacagcttg tggaaaattc ttgctatcta tggtaaaata
     6181 tctctagtat taaactttta aatagtaaag gaggagaaag gattgaaaga aaaacaattt
     6241 tggaatcgta tattagaatt tgcacaagaa agactgactc gatccatgta tgatttctat
     6301 gctattcaag ctgaactcat caaggtagag gaaaatgttg ccactatatt tctacctcgc
     6361 tctgaaatgg aaatggtctg ggaaaaacaa ctaaaagata ttattgtagt agctggtttt
     6421 gaaatttatg acgctgaaat aactccccac tatattttca ccaaacctca agatacgact
     6481 agctcacaag ttgaagaagc tacaaattta actctttatg actatagtcc aaagttagta
     6541 tctattcctt attcagatac gggattaaaa gaaaagtata cctttgataa ctttattcaa
     6601 ggggatggaa atgtttgggc tgtatcagcc gctttagctg tctctgaaga tttggctctg
     6661 acctataacc ctctttttat ctatggagga ccaggccttg gtaagactca cttattaaac
     6721 gctattggaa atgaaattct aaaaaatatt cctaatgcgc gtgttaaata tatccctgcc
     6781 gaaagcttta ttaatgactt tcttgatcac ctaagacttg gggaaatgga aaagtttaaa
     6841 aagacctatc gtagtcttga tcttttgtta atcgatgata tacagtcact cagcggaaaa
     6901 aaagtcgcaa ctcaggaaga atttttcaat acctttaacg cccttcatga caagcaaaaa
     6961 cagattgtcc taacgagtga tcgtagtcca aaacatctag aagggctcga ggagaggctt
     7021 gtcacgcgtt ttagttgggg attgacacaa actatcacac cccctgactt tgaaacacgt
     7081 attgccattt tacaaagtaa aacggaacat ttaggctaca atttccaaag tgatactcta
     7141 gaatacctag ctgggcaatt tgattcaaat gttcgagatc ttgagggagc catcaacgac
     7201 atcactttaa ttgccagagt aaaaaaaatc aaggatatca ctattgatat tgctgcagaa
     7261 gccattagag cccgcaaaca agatgttagc caaatgctcg tcatcccaat tgataaaatc
     7321 caaactgaag ttggtaactt ttatggtgtt agtatcaaag aaatgaaggg aagtagacgc
     7381 cttcaaaata ttgttttggc ccgtcaagta gccatgtatt tatctagaga actaacagat
     7441 aatagtcttc caaaaattgg gaaggaattt gggggaaaag atcataccac agtcattcat
     7501 gcccatgcca aaataaaatc tttgattgat caagacgata atttacgttt agaaattgaa
     7561 tcaatcaaaa agaaaatcaa ataatttgtg gataactttt agttttttat cttttttatc
     7621 cacatttttt aaacaagcta aaaaacttga tatgacttgt ttaaaggctg ttttccacag
     7681 atttcacaga ctctattatt actattatct ttctaatact a
//
LOCUS       DAVKJJ010000073         7533 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000073 DAVKJJ010000000
VERSION     DAVKJJ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7533
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.073"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(1..939)
                     /locus_tag="VW144_001872"
                     /pseudo
     CDS             complement(1..939)
                     /locus_tag="VW144_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1195..2055
                     /gene="speE"
                     /locus_tag="VW144_001873"
     CDS             1195..2055
                     /gene="speE"
                     /locus_tag="VW144_001873"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEU9601277.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEYDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
     gene            2052..3311
                     /locus_tag="VW144_001874"
     CDS             2052..3311
                     /locus_tag="VW144_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEU9601278.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVKKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            3311..4438
                     /gene="nspC"
                     /locus_tag="VW144_001875"
     CDS             3311..4438
                     /gene="nspC"
                     /locus_tag="VW144_001875"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEU9601279.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGSRCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            4435..5520
                     /gene="aguA"
                     /locus_tag="VW144_001876"
     CDS             4435..5520
                     /gene="aguA"
                     /locus_tag="VW144_001876"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEU9601280.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKDAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            5530..6405
                     /gene="aguB"
                     /locus_tag="VW144_001877"
     CDS             5530..6405
                     /gene="aguB"
                     /locus_tag="VW144_001877"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEU9601281.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(6586..7395)
                     /locus_tag="VW144_001878"
     CDS             complement(6586..7395)
                     /locus_tag="VW144_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9601282.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
BASE COUNT         2189 a         1475 c         1691 g         2178 t
ORIGIN      
        1 accgcagatg aggggaaccc cgtttgatca ttcttccagc taaatcaatc tgacctgact
       61 gataaataga agaatccagt ccagcgaaag cttgtaattg agcaggatta tcaaaggcat
      121 gaatatttcg aatctcggct aaaatgaccg cccctaaacg attctcaatc ccagtgaccg
      181 tcgtgatgac cgagttgaac tcagccatca agtcattgac acatgtttcc gccttgtcaa
      241 tgagcctctt gtaatgtttg atgatttcga attcacgagc aggagatgtt gttccgatag
      301 aacgaggtgc gactgagagg atatcctgaa ttttagaagc ggtcaatcgc ttaatttcta
      361 tcagcttatc aaatcctgcc tcaatccttt tctgaggatt agggtagcgt gtcaagagtt
      421 ggtaggtata ttctgaatgc tttccaacga ttttatccaa ctcaggaaag atgatatcaa
      481 gacaacgagt gtattgtact ttccaatcag actgtttttt cttgagacga tgaatatgtc
      541 tagtcagtat ttttagttct acttgccgat tatcgtgttg aaattgttca cgattggggt
      601 cagaaagaag tttaagagcg atgccatgag cgtctttctt atccgtttta gtcttgcgaa
      661 gtgataatga tttggcaaat tccttgatga gcaaaggagt gtaggtgtaa actttatatc
      721 cttgttcatg caggaagttc agtagattaa aggcataatg tccggtattt tcaagagcga
      781 tgagacagtc ttggttgagt tgtcgaagag acagatctaa gagttcaaaa ccagctttat
      841 tatttgaaaa agtgagtggt ttaagaacag ttttcctcga acattcaagg ctgtaacatc
      901 gtgtttattt ttagcgacat caatgcccac ataaagcatg ggagtacctc cagatatagt
      961 atttcaagtc tacttggtta tccacgaact ttttgccttg ttaccttaga cgagataaaa
     1021 cgtctatgcg ttatcaaact cattaccaat tgaaacaaaa agctgtggtt agaccctttc
     1081 ggaaatcgtc aagcgattgg aggaaatgaa ctaatccaca gtggcttatt ccaagtatac
     1141 cacttgggct ttggcagtag ctaactgcgc taaatataat ataaggagaa atagatggat
     1201 ttatggtttt ctgaagttca tactccagat gtcaaattgt ctctgagaac agccaagcaa
     1261 ctttacgctg gaaaaagtga atggcaggat atcgaagtct tggatacgcc agcttttggg
     1321 aaaatactga ttttaaatgg ccatgtcttg ttctcagatg cggatgattt cgtctacaat
     1381 gaaatgaccg ttcacgttcc catggctgtc cacccaaatc caaagaaagt attggttatt
     1441 gggggtggtg acggcggtgt tgcccaagta ttaacactct atcctgaact ggaacaaatc
     1501 gatatcgtgg aaccggatga gatgttggtc gaggtctgtc gtgagtattt cccagacttt
     1561 gctgcaggcc tagatgatcc tcgtgttacc atttactacc aaaatgggct acgctttttg
     1621 cgaaactgcg aatatgacta tgatattatc atcaacgatg cgacagatcc atttggccat
     1681 acggaaggac tctttaccaa ggaattctac ggcaatagtt atcgagctct gaaggaagac
     1741 ggcatcatga tttaccagca tgggagtccg ttctttgacg aggatgagtc ggcctgccga
     1801 agcatgcacc gcaaggtcaa tcaagccttt ccaatcagtc gggtttatca ggcccatatt
     1861 ccaactagcc cagctggcta ttggttgttt ggatttgcat cgaaaaaata ccaccctgtc
     1921 aaagattttg acaaggaagg ctggaaaaaa cgccagcttt tcacagaata ctacactgca
     1981 aacttacacg tgggagcctt tatgttgccc aagtatgttg aggacatttt agaagaagag
     2041 gaaggaaaaa aatgagtcgt ttactagtta ttggttgtgg gggcgttgcc caagttgcta
     2101 tttcaaagat ttgtcaagat agcgaaacat ttacagagat tatgattgct agccgtacca
     2161 agtcaaaatg cgatgacttg aaagctaaac tggaaggcaa aacaagtaca aagattgaga
     2221 cagctgctct tgatgctgac aaggttgaag aagtgattgc tctgattgaa agctacaaac
     2281 cagaagctgt tttgaatgta gctctgcctt atcaagattt aaccattatg gatgcttgtt
     2341 tggcaacagg tgttcactat atcgatacag ccaactacga agcagaagac acagaagacc
     2401 ctgagtggcg tgctatctac gaaaaacgtt gtaaggaact tggttttaca gcctactttg
     2461 actactcatg gcagtgggct tatcaagaga aattcaaaga agcaggcttg actgctcttc
     2521 ttggttctgg ttttgaccca ggtgtaacta gtgtcttttc agcttatgcc ctcaaacact
     2581 attttgatga aatccattat atcgacattt tagactgtaa tggcggtgac cacggttatc
     2641 catttgcaac caactttaat ccagaaatta atctccgtga ggtttctgcg ccaggttctt
     2701 actgggaaga tgggaaatgg gtcgaagtcg aagctatgtc tatcaagcgt gagtatgatt
     2761 tccctcaagt tggacaaaaa gatatgtatc tccttcacca tgaagaaatc gaatcattgg
     2821 ccaagaacat tccaggtgtc aaacgcattc gtttctttat gacttttggt caatcttact
     2881 tgacgcacat gaaatgtctt gaaaatgttg gactccttcg tacggatacc attaacttta
     2941 acggccaaga aattgttcca attcaatttt tgaaagcctt gcttccagat cctgctagcc
     3001 ttggtccacg tactgtaggt aaaaccaata ttggatgtat ctttacaggt gtcaaagacg
     3061 gtgtcaaaaa gactatctat atctacaatg tctgcgacca tcaggaatgt tacgcagagg
     3121 ttggttcgca agctatttct tatacgacag gagttccagc catgattggg acaaaattag
     3181 tcatgaacgg aacttggaaa caagctggag tgtataacct tgaggagtta gatccagatc
     3241 cattcatgga agctttgaat gagtatggtt tgccatgggt tgtggttgaa aatccacaaa
     3301 tggtggacta atgaagttag aacaagtacc aacaccagcc tatgttattg acttggccaa
     3361 gttagaagct aattgccgca ttctacaata tgtacaagaa gaggccggtt gcaaggtctt
     3421 gcttgcccag aaggcatatt ccctctacaa aacttatccc ttgattagcc agtatctatc
     3481 aggtacgaca gctagtggac tctatgaggc caaattggca agggaagaat ttcctggtga
     3541 agtccatgtt tttgcaccag ctttcaagga tgaggacatg gaggaattgc ttgggataac
     3601 agaccatatc gtctttaact cagagagaca gttgcgtaag catggttcgc gttgtcgaga
     3661 ggctggtgtc agtgttggtt tgcgcctcaa ccctcagtgt tcaactcaag gagatcacgc
     3721 gctctatgac ccttgtgcac caggttctcg ctttggagtt actatagaca agattccgag
     3781 tgatttgcta gatttggttg acggacttca ttttcatacc ctttgcgagc agggagcaga
     3841 tgatttacaa acaactttga aagcagtaga agaacagttt ggtccctact tacatgaggt
     3901 aaaatggctc aatatgggtg gtggtcatca tattacaaga gaaggttacg atgtggattt
     3961 gctgatttca gaaatcaagc gtatccgaaa aacttacaat cttgaaatct atatcgagcc
     4021 tggtgaagcc attgcgctta atgcgggtta tttagcaact gaggtattag atattgtaga
     4081 aaacggtatg gaaatcttgg ttttagacgc ctctgcgacc tgccatatgc ctgatgtact
     4141 tgagatgccc tatcgtccac ctttgagaaa tggctttgag gcacaggaaa aagcccatac
     4201 ctacagactt tcttctaata cctgtctgac gggcgatgtg attggtgatt atagttttga
     4261 aaatccagtc caaatcggag acagacttta ttttcaagac atggccattt attcttttgt
     4321 caaaaataat acctttaatg gtattggatt gccaagtctc tatctcatgg acgaacaggg
     4381 agactgtagc ttactcaaag cttttggcta tcaagacttt aaagggagat tatcatgatg
     4441 gacagtccaa aaaaattagg ctatcacatg ccagcagagt acgaacccca tcatggtacc
     4501 ctcatgatat ggccgactcg accaggatca tggccttttc aaggaaagga tgctaaaaga
     4561 gcatttactc agatcatcga gactatagca gaaggggaaa gagtctatct tttggtggag
     4621 caggcctatc tatctgaagc ccaatcctat cttggagaca aggttgttta tttagacatt
     4681 cccaccaatg atgcctgggc gcgtgatact ggcccaacca ttctcgtcaa tgacaaaggt
     4741 aagaaattag ccgtggattg ggccttcaat gcttggggag gcacctatga tggtctttat
     4801 caagattatg aagaggatga ccaagtagcc agtcgttttg ctgaggcctt ggaaaggcct
     4861 gtctatgatg ctaaaccttt tgtactcgaa ggaggcgcaa tccatagcga tggtcaagga
     4921 actattctcg taactgaaag ttgcttgctt agtcctggtc gcaatcctaa cttgactaaa
     4981 gaggagattg aaaacacatt attagaaagt cttggtgctg aaaaagttat ttggcttcct
     5041 tatggtattt atcaggatga aaccaatgaa cacgtcgata atgttgctgc ctttgttggt
     5101 cctgctgagg ttgttttggc ttggacagat gacgaaaatg atccccagta tgccatgtca
     5161 aaagcagatc tcgaactctt agaacaggaa acagatgcaa aaggttgtca cttcaccatt
     5221 cataaattgc ctatccctgc agttcgacaa gttgtgacag aagaagattt gccaggctac
     5281 atctatgaag aaggagaaga agagcgatac gcaggtgaac gactagcagc ttcctacgta
     5341 aacttttata tcgccaacaa ggctgtcttg gttccacagt ttgaggatgt aaacgaccaa
     5401 gtggccttag atatcctcag caagtgtttc ccagaccgta aagttgtcgg aataccagcc
     5461 agagatattc tcttaggtgg tggcaatatc cactgtatca cccaacaaat tccagaatag
     5521 gagaaaaaga tgagaaatgt aagagttgca accattcaga tgcaatgcgc taaggatgtg
     5581 gcaacaaata tccaaaccgc agagcgttta gtacgtcagg ctgctgagca aggagcccaa
     5641 attattctct tgcccgagtt gtttgaacat ccctatttct gtcaggaacg tcagtatgac
     5701 tactaccagt atgcccaatc tgtagcggaa aatactgcca ttcagcattt taaggtgatt
     5761 gctaaggaac tacaagttgt tttaccaatc agtttctatg aaaaagatgg taatgtcttg
     5821 tataactcta ttgccgtcat tgatgcagat ggggaagtgc tgggcgttta tcgaaagacc
     5881 catataccag atgaccatta ttatcaagaa aaattctatt tcacgcctgg taacactggt
     5941 ttcaaggtct ggaatactcg ctatgctaag attggtatcg gtatctgttg ggatcaatgg
     6001 ttccctgaaa cagcgcgctg tcttgcattg aatggtgctg aattgctctt ttatcctaca
     6061 gctatcggtt cagagccaat tttggataca gatagttgtg gtcactggca acgtactatg
     6121 caagggcacg cagcagcgaa tattgttcca gtcatcgcag ccaatcgtta tggtttagag
     6181 gaggttactc ctagtgagga aaatggcgga cagagttcca gtcttgactt ctacggttcc
     6241 tcctttatga cggatgaaac aggagctatt ctagaacgag ctgaaagaca agaagaagct
     6301 gttctgttag ctacttatga cctagacaag ggagcaagtg aacgtctaaa ctggggcttg
     6361 tttcgagata gaagaccaga aatgtataga caaattacag attagtgtgg gagaaatgag
     6421 agattcattc tgctagacta acttcttatt agcaactata agatactatg gcatctagta
     6481 aatcgatttt tatgattcgc tattcttgtc tattgattag tccgtatttt aaaatattag
     6541 caaaaaagca aatagcagta acttctgtct atttgctttt cttttttata gaatatattt
     6601 ctcaatagca cgcgcaacgc cgtcttcttc gttgcttgag gtaacggcat ccgcaagaga
     6661 tttgacataa tcgctggcat ttcccattgc aatcccaagc cctgcaaact ggagcatttc
     6721 gatatcgtta ttagcatcgc ccatggccat aatctctgag gaatcaatct tcaaaatctc
     6781 agctagtcgt gaaagagcag tagcctttgt cgttccaagt ggcattgctt cataaatgac
     6841 aggctgcgaa cgaactccac tgaatcgttg gcaaagctct tcagcaaaac gctgctcaaa
     6901 atcgtctgtt tgttcttttg ttcctaaaaa cataccttgg aacatccgga actttccact
     6961 agtcgcttcc tcaagagaaa tttcagtcag gtctgaaaat actagtttag catcattttc
     7021 aataacttga ttgggcttgc caccgagaac aaaataatgt gactcgtcaa aaagtgtcaa
     7081 ctgaacatca ctcttttcag caaggtcata gaggtattcg atgtcagctg gactcagttc
     7141 tttccagtca actagacccc aatcactggt ttggtgagtt gaacaaccgt tgttaacaat
     7201 aacatattcg ttctggaggt caagccccag ttttttgtag taggggagga caccgaaaag
     7261 ggggcgaccc gtacagagaa ccagtttgac acctttttca atggctttgt gaatagcagt
     7321 aatgtgagct tgtgggattt ccttggcttc attgaggagg gtgccgtcca tatccaaggc
     7381 tagtagttta atcataggtc ttccttcttt atcttttggt attattatag catattttgg
     7441 ataagaaatt gatagaaagc ttgagactaa ttgattttat agtttaagat gttttgatga
     7501 caattcatga tttgaagagg atatttggca aag
//
LOCUS       DAVKJJ010000074         7457 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000074 DAVKJJ010000000
VERSION     DAVKJJ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7457
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.074"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            120..275
                     /locus_tag="VW144_001879"
                     /pseudo
     CDS             120..275
                     /locus_tag="VW144_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            475..3750
                     /gene="rexB"
                     /locus_tag="VW144_001880"
     CDS             475..3750
                     /gene="rexB"
                     /locus_tag="VW144_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEU9601283.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPVKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRTDLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQIFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDLQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3747..7397
                     /gene="addA"
                     /locus_tag="VW144_001881"
     CDS             3747..7397
                     /gene="addA"
                     /locus_tag="VW144_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEU9601284.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMVERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDINHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNMHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRNRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKAHENLYEKLVNAQKKAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKPHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTALGQEIFANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
BASE COUNT         2273 a         1568 c         1585 g         2031 t
ORIGIN      
        1 gattgaaata agatacgaac agcttgatta gggaaatcaa attaatttct agaaatattt
       61 tagcatccac gatgtactat tatagtttca atttactata gttcaatcat tctatcaaga
      121 atattgaata tataaaggca cgtaatatta aaacttcttc tgatatcgaa ttagagtccg
      181 atgttgatgg cgataaatca gacaatctac ccgtcgacat taagatactg ggacaacaca
      241 ttgaagtgta ttccgtacct aagaaacaat cataaaactc aaagtcttgt taccgaggct
      301 tttttattat gtgattttca gtagccagac ttattctatc taatacaagt aaagctaagt
      361 atttaagatt tagcagatga tgaagaaaag aaaggatggc ctaaatattt taacagattt
      421 acgaggctaa tcatggtata atatagggtg atgaaaaatt ccaacgaggc tgagatgaaa
      481 ttactttata ctgatattcg aacttctttg acagaaattc taacaagaga ggcagaagag
      541 ctagttgcag ctggcaagcg ggtcttctac attgccccca actctctttc ttttgaaaag
      601 gaacgcgccg tgctggaatg cttgtcccag caggcttctt tttctattac cgtcacgcgc
      661 tttgctcaaa tggctcgtta tctggtcctg aatgatttac cagttaaaac tactcttgat
      721 gatatcggtc ttgggttggc cttttacaaa tgccttgccg aactcgatcc caaggacttg
      781 cgtgtttatg gcgctattaa gcaggatcct caattgatcc agcagttaat tgagctttac
      841 catgagatga ccaaatctca gatgagtttt ttggacttgg agaatttaac agatgaggat
      901 aagaggacgg atttactctt gatttttgag aaagtaacag cctatcttaa tcaaggtcag
      961 ttagcccagg gaagtcagtt gtcccatttg attgaggcta ttgagaatga caaggtaagt
     1021 agtgatttta atcaaatcac cttggtcatt gacggcttta ctcgtttttc tgctgaggaa
     1081 gagcgggttg tggacttact tcacggcaaa ggtgttgaga ttgttatcgg ggcttatgct
     1141 agtaagaaag cctataccag tccttttagc gagggcaatc tctaccaagc cagcgtaaaa
     1201 tttctccatc atctggcttc taaataccaa acgcctgctc aggactgttc tcaaactcat
     1261 gagaagatgg atagttttga caaggcctct cgtttgttgg agtcttctta tgacttttca
     1321 gaactcgctt tggatgtcga tgagaaagac cgtgaaaatt tacaaatctg gtcttgtttg
     1381 acgcaaaagg aggagttgga gctagtagcc cgtagcattc gtcagaaatt acatgagaac
     1441 tcagacctga gctacaagca ttttcgtatt ctcttggggg atgtagcttc ttaccagtta
     1501 tctctcaaaa ccatttttga ccagtatcag attccttttt atcttggtag aagcgaagcc
     1561 atggctcatc atcccttgac tcagtttgtc gagtctattt tagctttaaa acgttaccgt
     1621 tttcgtcagg aggatttgat taatcttctt agaactgatt tgtatactga cctcagtcag
     1681 tctgatattg atgcttttga gcaatatatc cgctatcttg gtatcaatgg cttgccagcc
     1741 tttcagcaaa tcttcaccaa atcccaccat ggaaaattta atcttgagcg tttgaatgtc
     1801 ctccgcctga gaattttagc acctcttgaa accctctttg ccagccgaaa acaaaaggct
     1861 gaaaacctcc tacaaaaatg gagtgtcttt ctaaaagaag gagctgtgac caagcagtta
     1921 caagatttga caaccacttt ggaagctgta gaacaggaaa gacaagccga agtttggaag
     1981 gctttctgcc atgttttaga acaatttgcg actgtttttg ctggttcaca ggttagtctg
     2041 gaagacttcc tagccttgct ccattctgga atgagtttgt cccaataccg taccattcca
     2101 gcaacagtgg acactgttct ggtgcagagt tacgatttga ttgcaccatt gactgctgac
     2161 tttgtctatg ctattggact aactcaggac aatttaccaa aaatttctca aaacaccagt
     2221 cttctgacag atgaagaaag gcaaaatcta aaccaagcga ccgaagaagg cgttcaatta
     2281 ctgattgcca gcagtgaaaa tctcaagaaa aatcgctaca ctatgctttc cttggtcaat
     2341 tctgctcgta agcagttgtt cttgtcggct ccaagccttt ttaacgaaag tgaaagtaag
     2401 gaatctgcct atcttcaaga gttgatccat tttggattta ggcggagaga gaagaggatg
     2461 aatcacaaag gactgtctaa ggaggatatg gggtcctatc acagtctttt gtctagtctg
     2521 gttgcctatc accagcaggg tgagatgagc gatactgagc aagatttgac ttttgtcaag
     2581 gttctgtcgc gtgtcatagg taaaaaacta gatctgcaag gtctggaaaa tccagctatc
     2641 ccaaccagtc caagcagcaa gaccttagcc aaggacacct tgcaagctct ctatcctgcc
     2701 aaacaggagt tttacctgtc tacgtcgggt ttgacagagt tttatcgcaa tgaatacagt
     2761 tatttcctac gctacgtttt aggcttgcag gaggaattac gtttgcgtcc tgatgcccgt
     2821 agtcacggga atttcttgca tcgtatcttt gaacgcgcct tacagttgcc taatgaagat
     2881 tcctttgacc aacgtctaga acaagctatt caagaaacca gtcaagaacg cgaatttgaa
     2941 gctatttatc aagaaagttt ggaagcccag tttaccaagg aagttttgct tgatgttgca
     3001 cggacgactg gacatattct ccgacacaat ccagccatcg aaaccatcaa agaagaagca
     3061 aattttggtg gaaaagacca agcctttatt caattagaca atggacgcag tgtctttgta
     3121 cgaggcaagg tggaccggat tgaccgtttg aaagctaatg gagcgatagg agtagtagac
     3181 tacaaatcca gtctgactca gttccagttt cctcatttct ttaatgggct caattctcag
     3241 ttaccaacct atcttgctgc cctaaaaaga gaaggggagc agaacttttt cggcgccatg
     3301 tacttggaaa tggctgaacc tgtccaatct ctgatggcgg taaaaagtct ggcaggagca
     3361 gtggtagaag ccagcaaatc tatgaaatac caagggctct tcttggaaaa agaaagcagt
     3421 tatttaggcg aattttataa caaaaacaag gctaatcaac tgacagatga ggaatttcag
     3481 ctcctactgg actacaatgc ctatctttac aagaaagctg ctgagaagat tttagcaggc
     3541 cggttcgcca tcaatcctta tactgaaaat ggcagaagca ttgccccata cgtccagcaa
     3601 catcaggcta ttacaggctt tgaagccaat taccatctgg gccaagcccg tttcctagaa
     3661 aagttggacc tagctgatgg caagcgtctg gtcggagaaa aactcaagca agcttggttt
     3721 gaaaaaataa gagaggagtt aaatcgatga agcttattcc ctttttaagt gaggaggaga
     3781 ttcaaaaact gcaagaagca gaagcaaatt cgagcaagga acagaagaaa actgccgaac
     3841 aaatcgaagc tatctacact tctggtcaga atatcctggt ctcagcatcg gctggttctg
     3901 gaaagacctt tgtcatggta gagcgcattc tggaccaatt ggcgcgtggt gtcgaaattt
     3961 ctcaactctt tatctcaacc tttaccgtca aggctgcaac tgaacttaaa gaacgtttag
     4021 agaaaaaaat cagcaagaaa atccaagaaa cagatgatgt cgatctcaaa caacacttgg
     4081 gtcgccagtt ggcagaccta cccaacgctg ccatcggaac catggactct ttcacacaaa
     4141 aattccttgg caaacatggt tatctgcttg atattgcacc taatttccgt attttacaaa
     4201 accaaagcga gcaacttctt ctcgaaaacg aagtctttca tgaggtcttt gaagcgcatt
     4261 accaaggtaa acagaaagag agctttagtc atttgctgaa aaactttgct gggcgtggca
     4321 aggacgaacg gggtctgcgg cagcaagtct ataaaatcta tgacttcctc caatccacca
     4381 gtaatcctca aaagtggctg agtgaatctt tcctcaaagg ctttgagaaa gctgatttta
     4441 ccagtgaaaa agaaaaactg actgagcaaa tcaaacaagc cctctgggat ttggaaagct
     4501 ttttccgtta ccatctggat aacgatgcca aggagtttgc aaaggctgcc tatttagaaa
     4561 atgttcagtt aattctggat gaaattggct ccctaaatca ggagttcgat agtcaggctt
     4621 atcaggcagt acttgcgcgt gttgtcgcca tctctaagga gaaaaacggt cgagctctga
     4681 ctaatgccag ccgtaaggct gatttgaagc ccctggctga tgcctacaac gaagagagaa
     4741 agacccagtt tactaaacta ggacaattat cagaccagat agcgattctc gactatcaag
     4801 aacgttatca tggagacact tggaaactag ctaaaacctt ccaatctttc atgagcgatt
     4861 ttgtagaggc ttatcgtcag agaaaacgac aggaaaatgc ctttgaattc gctgatatca
     4921 gccattacac cattgagatt ttagaaaatt tcccacaagt tcgtgagtct tatcaggagc
     4981 gcttccatga agtcatggtc gatgagtatc aggatatcaa ccatattcaa gaacggatgc
     5041 tggaattgtt gtctaatggc cacaatcgct ttatggtggg agatatcaag caatccatct
     5101 atcgtttccg tcaggcagac ccgcagattt tcaatgagaa attccaacgc tatgcgcaaa
     5161 atcctcaaga aggcaagctc attctcctca aggaaaattt ccgtagtagt tcagaagtgc
     5221 tgtcagcaac caatgatgtc tttgaacgtc tcatggacca agaggtcggc gaaatcaact
     5281 atgataacat gcaccagctt gtttttgcca ataccaaact gactcccaat ccagacaaca
     5341 aggccgaatt tctcctctac gacaaggacg atacaggtga ggaagaagag agtcaaacag
     5401 aaacgaaact aacaggcgaa atgcgcttag ttatcaagga gatccttaaa cttcatcagg
     5461 aaaaaggtgt tgcctttaag gaaattgccc ttctgacctc tagtcgtaat cgtaatgacc
     5521 aaattctact tgccctgtct gagtacggga ttcctgtcaa aactgacgga gagcaaaaca
     5581 attatctcca atccctagaa gtgcaagtca tgctagacac ccttcgtgtc attcacaatc
     5641 ccctgcaaga ctacgccttg gttgccctta tgaagtctcc aatgtttggt tttgatgagg
     5701 atgagctagc acgtttgtcc cttcagaaag cagaggataa agcccacgaa aatctctatg
     5761 agaaactagt caatgcacaa aaaaaggcaa gtagtcaaaa aggcttgatt cacacagctc
     5821 tagctgaaaa actaaagcaa ttcatggata tcctagcttc ttggcgcttg tatgccaaac
     5881 cccactctct ctatgacttg atttggaaga tttacaacga ccgtttttat tatgactatg
     5941 ttggggcttt gccgaatggt cctgctaggc aggccaatct ctatgcccta gcactgcgtg
     6001 ctgatcaatt tgaaaagagc aatttcaaag gtttgtcgcg ttttattcgt atgattgacc
     6061 aagtcttaga agcccagcac gatttggcaa gcgtggccgt cgcaccgcca aaagatgcag
     6121 tagagctcat gaccatccac aagagtaaag ggctggagtt tccttacgtc tttatcctca
     6181 atatggatca agatttcaac aaacaagact ctatgtcaga agtcattctc agtcgtcaga
     6241 atggtcttgg tgtcaaatat attgccaaga tggagacagg ggcagtagaa gaccactatc
     6301 ctaaaaccat caaactctcc attcctagtc tgacctatag gcagaacgaa gaggaattac
     6361 agctagcaag ctattctgag cagatgcgtt tgctgtatgt tgctatgacg cgggctgaga
     6421 aaaagctcta tcttgtcggc aagggttctc gtgaaaagct ggaatccaag gaatacccag
     6481 cagccaaaaa tgggaaacta aatagcaata ctagactgca agcacggaat ttccaagatt
     6541 ggctttgggc tatcagtaaa gtgtttacta aggacaagct caactttagt tatcgtttta
     6601 ttggcgaaga tcagttgacc agagaagcta tcggagagtt ggaaaccaag agtcctctcc
     6661 aagatagctc ccaagcagac aatcgtcagt cagataccat caaagaagct ctggaaatgc
     6721 tgaaggaggt ggaagtttat aatactcttc accgcgcagc tattgaactt cctagtgttc
     6781 aaaccccaag tcaaatcaag aaattctacg aaccagttat ggatatggaa ggtgtcgaga
     6841 ttgctggtca aggtcagtca gtaggcaaga aaatcagctt cgatttgcca gatttttcaa
     6901 ccaaagaaaa ggtaactgga gctgagattg gtagtgctac tcacgaactc atgcagagaa
     6961 ttgacctcag ccagcaacta acccttgcta gcctaacaga aacactcaaa caagttcaaa
     7021 ctagccaagc tgtcagagac aagatcaatc ttgataaaat tcttgctttc tttgacacag
     7081 cactcggtca ggaaattttt gctaataccg accatctcta tcgcgagcaa cctttctcca
     7141 tgctcaaacg agaccaaaag agtcaggaag actttgttgt ccgtggtatc cttgatggct
     7201 atctgcttta cgaaaacaaa attgttttgt tcgactacaa gacagaccgc tatgatgaac
     7261 caagtcaact cgtagaccgc tatcgtggtc agttagctct atacgaagag gctttatcac
     7321 gagcctattc gattgaaaat attgaaaaat acttgatttt actcggtaaa gacgaggttc
     7381 aagttgtaaa agtataactt atactcttcg aaaatctctt caaaccacgt cagcttcgcc
     7441 ttgctgtata tatggtt
//
LOCUS       DAVKJJ010000075         7291 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000075 DAVKJJ010000000
VERSION     DAVKJJ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7291
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.075"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            <1..4005
                     /locus_tag="VW144_001882"
     CDS             <1..4005
                     /locus_tag="VW144_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEU9601285.1"
                     /translation="SRLTEGLRTKADISSLNVTAENIRQSVKSLETDTQNKLNQKLSQ
                     AEFEVRAGSIRQEILNATKDKASKSELTQTAEELASRIASVQASGRNLFLNSLFKQDI
                     SKTGIWTTSTYTATIDSESKYLGHKALKIIGLNPSGRDGGNPKVTYPALGQFGKVIPG
                     STTNQDVTISFYAKANKNGIMLRSRLGNIGYKTGNVTLSTEIKRYVVHIPKGWTNESK
                     QTTNEWLFNFNQEGTIWIWMPKFEISDVDTSYSEAPEDIEGQISTVESTFKQRANSLD
                     AGVRSLTEGLRTKVDISSLNVTAENIRQSVKSLETDTQNKLNQKLSQAEFEVRAGSIR
                     QEILNATKDKADKTLVVSEAGKLREEFSKMKVGGRNLWIKSKTVGAVIEKLPENHVTG
                     QKECYRLENNSTLTFNIEPDFSSRLYQKVTFSAWVKYENVVQGRNFWNVFNCFKHYLF
                     RKNSETGVQSGPDYATLGMYKGSADWKYITFTYDYSEKTNFDQLKTSLRFNLEGATSG
                     TAWVTGIKVEIGSVATDWSPAPEDADGLITEAKATFERTAQGLRTDLSAIQEYVNKDG
                     QRQEALQRYTREESTRQAIAVRELVNRDFVGKATYQEDVKGINQRIEAVKTSANKDIA
                     SQIASYRQSVDGKFTDISSQITTYKQDVGGQISGLSNRLTSSEQGTTTQISNLSNRIN
                     SNKQGADNQISNLKTQVATNKDNAERQMGRISDQVSANKANADSQFANVTNQLVRKVE
                     TTDFQRVKETSKLYERILGNTENGIADKVARMALTNQLFQVEVGKVAKGGRNYIRNGQ
                     FKNGSKNWLEYQSVNFGLNFNYQHSQNPNNRNRPGLHFYHDSQDVANFFGIQQSFAFD
                     GVRGEKVSVSLLVSKDGGDSNSGLKVALHYIKNKNIIGQEWQNIPSPQITSKYKRFTF
                     TFTLSDDVENLNLMLFGEKGKTINLYVTDVQLERGSVATDYKEAPEDTDEAIRSVQSQ
                     LTGSWAVQNINSAGDIISGINLGANGHNRFVGKLTHITGETLIDRAVIKSAMVDKLKT
                     ANFEAGSVTTTILDAEAVTAEKLKVDNALIRKLTANDAFIDQLISKRIFSTKVESVIS
                     SSTFLEAYQGRIGGFTLGQFDQGGGRWISGVNQFSVGMGNGAGYGVRTAFWANWGNNW
                     NYAGPKAWNVNTDGKMYCRNEVGFYDQVDFSNSSRANFYGNTTFSRSPVFSNGIELGS
                     KDVLGDGWNPKGGRNAVVWWNQVGSGSVKYWMEQKSDRRLKENITDTAVKALDKINRL
                     RMVAFDFIENKKHEEIGLIAQEAETIVPKIVSRDPENPDGYLHIDYTALVPYLIKAIQ
                     ELNQKIEKMEKTIA"
     gene            4099..4302
                     /locus_tag="VW144_001883"
     CDS             4099..4302
                     /locus_tag="VW144_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601286.1"
                     /translation="MNTEQLNQALQMTISEMSTTSTNSMITSNILSIQLNEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            4305..4655
                     /locus_tag="VW144_001884"
     CDS             4305..4655
                     /locus_tag="VW144_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601287.1"
                     /translation="MAETIQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQFKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTVPTVEPKE"
     gene            4664..5080
                     /locus_tag="VW144_001885"
     CDS             4664..5080
                     /locus_tag="VW144_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEU9601288.1"
                     /translation="MQIEFFNFLRSVVQTEDGLVLYALALIVSMEIIDFVTGTIAAII
                     NPDIEYKSKIGINGLFRKISGVLLLMILIPASVLLPEKTGFAFLYSIYLGYIAFTFQS
                     LIENYRKLKGNVTLFQPIVKVFQRLLEKDDDTKKGE"
     gene            5084..5416
                     /locus_tag="VW144_001886"
     CDS             5084..5416
                     /locus_tag="VW144_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEU9601289.1"
                     /translation="MQQITEIITNGAISILVILAGIAVKAVKDYLVQKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKVRAELTKYNISMTDKNLDTFVESAVKQ
                     MNDVWKGR"
     gene            5420..6376
                     /locus_tag="VW144_001887"
     CDS             5420..6376
                     /locus_tag="VW144_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9601290.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTVQNEADYHWR
                     KDPELGFFSHVVGNGRVMQVGPVNNGSWDVGGGWNAESYAAVELIESHSTKEEFMADY
                     RLYIELLRNLADEAGLPKTLDTDDLAGIKTHEYCTNNQPNNHSDHVDPYPYLTKWGIS
                     REQFKQDIENGLSAATGWQKNGTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLSD
                     RWKKHTDGNWYYFDQSGEMATGWKKIAEKWYYFDVEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(6647..6826)
                     /locus_tag="VW144_001888"
     CDS             complement(6647..6826)
                     /locus_tag="VW144_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601291.1"
                     /translation="MREVIQELLDSSMSTSAISQGAGVPWTTVSDLRKGKTSMDKMAL
                     LTAEKLYEFATTDKQ"
     gene            complement(6968..7117)
                     /locus_tag="VW144_001889"
     CDS             complement(6968..7117)
                     /locus_tag="VW144_001889"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601292.1"
                     /translation="MNKEDWLRLLEKAIDNIPETVTAIASLVTAITVARQNKKRKPKS
                     RQRKR"
BASE COUNT         2356 a         1355 c         1647 g         1933 t
ORIGIN      
        1 agccgtctga ctgaaggcct tagaaccaaa gcggatatca gctcactcaa cgtgactgct
       61 gagaatatcc ggcaatctgt gaagagcctt gagacagaca cgcagaacaa gctaaatcag
      121 aagttgagtc aggctgaatt tgaggtgcga gctggctcta tccgtcagga aatcctgaac
      181 gcaaccaagg ataaagccag caagtcagaa cttacgcaga cagctgagga gctagctagt
      241 cggattgcga gtgtgcaggc atccggtcga aatctattct tgaactcact attcaagcag
      301 gatatttcaa aaacaggaat ttggacaacg agtacatata cggctactat cgatagcgaa
      361 agtaagtatc ttggacacaa ggctcttaaa attataggtc tgaatccatc tggtcgtgat
      421 ggaggtaatc ccaaggttac ttatccagct ctgggtcaat tcgggaaagt aattcccgga
      481 agtacgacta atcaagatgt aaccattagt ttttatgcta aggcaaataa aaatggaata
      541 atgctaagat ctcgattagg gaatatcgga tataaaactg gaaatgtgac attgtcgaca
      601 gaaattaagc gatatgttgt ccatattcca aaaggttgga caaacgaatc caagcagacc
      661 acaaatgaat ggttgttcaa tttcaaccag gaaggaacca tttggatttg gatgccgaag
      721 tttgaaataa gcgatgtaga tacttcttat tcagaagctc ctgaagatat agaaggtcag
      781 atttcaacag tagaatcgac ctttaaacaa cgagccaact cgctcgacgc tggtgtgaga
      841 agcttgactg aagggcttag aaccaaagtg gatatcagct cactcaacgt gactgctgaa
      901 aatatccggc aatctgtgaa gagtcttgag acagacacgc agaacaagct aaatcagaag
      961 ttgagtcagg ctgaatttga ggtgcgagct ggctctatcc gtcaggaaat cctgaacgca
     1021 accaaggata aagcagataa gactttagtt gtatctgaag ctgggaaatt gcgtgaagaa
     1081 ttttcaaaaa tgaaggtggg aggacggaat ctatggataa aatccaagac ggttggagct
     1141 gtaattgaaa aattacctga aaaccacgtc acaggtcaaa aagaatgcta taggctagag
     1201 aacaactcta ctttaacgtt caacattgaa ccagatttca gctcaaggtt gtatcaaaaa
     1261 gttactttta gcgcttgggt caagtacgaa aatgtagtcc aaggtcgaaa tttttggaat
     1321 gtatttaatt gcttcaaaca ttatcttttt agaaaaaata gtgagaccgg agtacagagt
     1381 ggtccagatt atgctacgct tggtatgtat aaaggttcgg cagattggaa atatattaca
     1441 ttcacttatg actactctga aaaaacaaat tttgatcaat tgaagacatc attgcgattc
     1501 aatcttgaag gtgctacaag cggtacagct tgggtaacag gaatcaaggt tgaaatcggt
     1561 agtgtggcga cggactggag tcctgcgcct gaggacgctg atggtctcat cactgaagct
     1621 aaggctacct ttgagcggac agctcagggc ttgcgaaccg atttatcagc tattcaggaa
     1681 tatgtaaata aagacggtca gcgacaggaa gccctacagc gctatactcg tgaggagagc
     1741 acgagacaag cgatagcagt ccgtgagctg gtcaatcgtg atttcgttgg taaggctact
     1801 tatcaagaag atgttaaggg tatcaatcaa aggattgaag ctgttaaaac tagtgcgaat
     1861 aaagacatcg ctagtcaaat cgctagctat cgtcaatctg tagatggtaa gttcacggat
     1921 atttcaagtc agataactac ttataagcaa gatgtgggcg gtcaaatcag tggcctttca
     1981 aatagactta caagcagtga gcaaggaacc actactcaga tttcaaatct ttcaaatcgg
     2041 ataaacagta ataaacaagg cgcagataat cagatttcaa atttaaagac tcaggtcgct
     2101 acaaacaagg ataatgctga acgacaaatg ggtagaatat ctgatcaggt ttctgcaaac
     2161 aaagcgaatg ctgatagtca atttgcgaat gtgaccaatc aactagtgcg aaaagtagag
     2221 actactgact tccagcgtgt taaggaaacc agtaaacttt acgagcggat tttgggcaat
     2281 actgaaaatg gaattgcgga taaggttgct cgcatggctc tgaccaatca actgtttcag
     2341 gttgaggttg ggaaggttgc caaaggtggt cgaaattata tcagaaatgg tcaatttaag
     2401 aacggttcga aaaactggct tgaatatcaa tctgttaatt ttggtttgaa tttcaactat
     2461 caacactctc aaaatcctaa taatcgaaat cgtccaggac tacacttcta tcacgattct
     2521 caagatgttg ctaatttttt tggaattcag caatcttttg catttgatgg tgttcgaggt
     2581 gagaaagtga gtgtatctct tcttgtttcg aaagatggtg gcgatagcaa cagtggcttg
     2641 aaagtcgctt tgcactacat caaaaacaaa aacattattg ggcaagagtg gcaaaatatc
     2701 ccaagtccac aaataacatc gaagtataag cgtttcacat ttacgtttac tttatcagac
     2761 gatgtagaga atctgaattt gatgttgttt ggtgaaaaag ggaagaccat caacctctat
     2821 gttacagatg ttcaactcga aagaggttct gtcgcgacgg attacaaaga agctcctgaa
     2881 gacacggacg aagcgattcg ctctgttcaa agccaactaa ctggctcatg ggcagttcaa
     2941 aacatcaact cggctggaga tatcatctct ggaatcaatc ttggcgccaa tggacataac
     3001 cgctttgttg ggaaattgac ccacatcact ggagagaccc tgattgacag agcagtcatc
     3061 aagtctgcca tggttgataa gctcaaaacg gccaattttg aagctggttc ggtcacgact
     3121 acgatattag acgctgaagc ggtaactgct gagaagttga aagttgacaa tgcgcttatt
     3181 agaaaattaa ctgcaaatga tgcttttatt gaccaactga tatctaaacg tatcttctct
     3241 actaaggttg agtccgtcat ttctagctca accttcctag aagcctatca aggccgaatc
     3301 ggtggattca cacttggtca atttgaccag ggtggcggtc gctggatttc aggtgtcaat
     3361 cagttctctg ttggtatggg gaatggtgcc gggtatggag tccggacagc cttctgggcg
     3421 aactggggaa ataattggaa ctatgccgga cctaaagcat ggaacgtcaa tactgatggg
     3481 aaaatgtact gtaggaatga agtcggtttt tatgatcaag tggatttttc gaattcatcg
     3541 agagcaaact tctatgggaa tactactttt tctcgttctc ctgtgttttc aaatggtatc
     3601 gaacttggaa gtaaagatgt gcttggtgat ggttggaatc ccaaaggcgg aaggaatgcg
     3661 gttgtttggt ggaatcaggt cggtagcggt agcgtgaagt attggatgga acaaaaatca
     3721 gacagacgct taaaagagaa catcacagat acagctgtga aagccttgga caaaatcaac
     3781 agattaagaa tggttgcatt tgatttcatc gaaaataaaa aacatgagga gattggtcta
     3841 atagctcaag aggctgaaac catcgttcca aaaattgtct cacgagatcc tgagaatcca
     3901 gatggctatc tgcatatcga ctataccgct ttagttcctt acttaatcaa ggctattcaa
     3961 gaattaaatc aaaaaataga aaaaatggag aaaacaatag catgaataac aacatgttga
     4021 ccaatatcgc acttaaagca atttaggagc ttgctcttga aaatagaaaa cgaacacaca
     4081 gattggagaa cttagaaaat gaacacagaa cagcttaacc aagccttaca aatgacaatt
     4141 agtgaaatgt caacaacttc aacaaattcg atgattacaa gtaatatctt gagtattcag
     4201 ttgaatgagc aaagggaaga gaatcaaaga cttcaagcac gagtggatga gctggaagct
     4261 ctgcttgatg aacaaactaa accagcagac aaaggagaat agacatggca gaaacaattc
     4321 aaaacacaga taacttacta gaccttacaa aaatcacaga accatttgat cttgcgagcg
     4381 ctttgcgcta catgaaagaa aatggagagt tcattcgttg caagaatgta agcgatgact
     4441 tctatatgta tcgtgacgtt caaaaacgtc ctgtgatcgt aaatggccgt cgccaattca
     4501 aggatgttga aaccgtttgg gcgttcaatc agtggggtgg tacaatcgca acaatcaacg
     4561 tagccgttct gttgaatcat gaattctata tcatgaaatt tgatgcagag ggcaatcctg
     4621 actggacggt tccaacggta gaacctaaag aataggaggt tgtatgcaaa ttgaattttt
     4681 caattttcta agaagtgtcg tacagactga agatggtttg gtattgtacg ctctagcact
     4741 gattgtctca atggaaatca ttgattttgt gacagggacg attgcggcaa ttatcaatcc
     4801 tgacatcgag tacaagagca aaatcggcat caatgggcta tttcgtaaga tttcaggggt
     4861 tctcttactg atgatcctca ttccggcgtc cgttttgttg cctgaaaaga caggttttgc
     4921 attcttgtac tcaatctatc tcgggtacat cgcatttact tttcaatctc tcattgaaaa
     4981 ttaccgcaaa ttaaaaggaa atgttactct ttttcagccg attgtaaaag tatttcagcg
     5041 attacttgaa aaagatgatg atacgaaaaa aggagaataa caaatgcaac aaattactga
     5101 aatcattact aatggagcaa tcagcatcct tgttattttg gctggtatcg cagtcaaggc
     5161 agtcaaggac tacctggttc aaaaaggtgg agaaaagacc atcaaaatcg ttgaaatctt
     5221 ggccaaaaat gcagtaaatg ccgtggagca ggtagctgct gaaactggct acaagggaga
     5281 tgaaaaactg gcacaggctc gtgctaaagt ccgtgctgag cttacaaaat acaatattag
     5341 catgactgac aaaaacttag acaccttcgt agagtcagca gtgaagcaga tgaatgacgt
     5401 atggaaagga cgatagggaa tggatatcga tagaaacaga ctacgtacag gcttgccaca
     5461 ggttggggtg cagccttatc gacaagtaca tgctcactca acaggtaacc gcaactcaac
     5521 cgtacagaat gaagcggatt atcactggcg gaaagaccca gaattaggtt ttttctcgca
     5581 cgttgttggg aacggccgtg tcatgcaagt aggtcctgtg aacaacggaa gttgggatgt
     5641 tgggggcggt tggaatgctg agagttacgc agcggttgaa ctgattgaaa gccattcaac
     5701 taaggaagag tttatggctg actaccgcct ctatatcgaa ttgctacgca atctagcgga
     5761 cgaagcaggc ttgccgaaga ctcttgatac agacgacttg gcaggtatca agacgcatga
     5821 atactgtacc aataaccaac caaacaacca ctcagatcat gtggatccat acccttactt
     5881 gacaaaatgg ggcattagcc gtgaacagtt taagcaagac atcgaaaacg gcttgagcgc
     5941 tgcaacaggc tggcagaaaa atggcactgg ctactggtac gtacactcag acggctctta
     6001 tccaaaagat aagtttgaga aaatcaacgg tacctggtat tatttcgatg gctcaggcta
     6061 tatgctttca gaccgctgga agaagcacac agacggtaat tggtactact ttgaccaatc
     6121 aggcgaaatg gctacaggct ggaagaaaat cgctgagaag tggtactatt ttgatgtaga
     6181 aggtgccatg aagacaggct gggtcaagta caaggacact tggtactact tagacgctaa
     6241 agaaggcgcc atggtatcaa atgcctttat ccagtcagcg gacggaacag gctggtacta
     6301 cctcaaacca gacggaacac tggcagacaa gccagagttc acagtagagc cagatggctt
     6361 gattacagtt aaataaaata gaaaggaaac tttctaaatt gttctttcac cgcaggctca
     6421 ggcttgcggt ttttttgttt gtctgaatca agaaaacgtc taaccaaccg acaccaatgt
     6481 cggtagcaaa ataaatggtt tgcctaaaaa attagagtta agttctattt ttcaaaaaca
     6541 cgcattttga acgattagaa acagaaatct aaatcctatt gttcaaaaaa acgtttacat
     6601 gaaaaatagg gaggtgtttt gttaaaaata aaaacagtga tcgaaatcac tgcttatcag
     6661 ttgtagcaaa ttcataaagt ttttctgctg tgagaagtgc cattttgtcc atgcttgttt
     6721 ttccttttct gaggtcagaa acagtagtcc atggaactcc agcaccttgg gaaatagcag
     6781 atgtagacat cgaactgtct aacaattctt gaataacttc tctcatctta tttgtccttt
     6841 ttatttttta gataaatata tacattgatt gcaattataa aaatagctat tgcactaacc
     6901 attgcatttc ctctttccat ttgataaaat agaggtgtaa ggggctttcg ccccaacctc
     6961 ttagcgttta cctttttctt tgacgggatt tcggtttacg ctttttgttt tgccttgcga
     7021 ccgttattgc ggtcactaga cttgcgatag cagttaccgt ttcaggaata ttgtctattg
     7081 ccttttcaag taacctgagc caatcttctt tgttcaactt catcaccccc tttccttatc
     7141 ttgattatat tatatcacgg tacaccgaga aagtcaagcg ttttgatgaa gtttttttaa
     7201 ttttttcaaa aaaaaaatag accttgtcca gaggtcgggg agttggaggg gacaccctcc
     7261 aagagtgttg atttaataag attttatttt a
//
LOCUS       DAVKJJ010000076         7014 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000076 DAVKJJ010000000
VERSION     DAVKJJ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7014
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.076"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(114..389)
                     /locus_tag="VW144_001890"
     CDS             complement(114..389)
                     /locus_tag="VW144_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU9601293.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(495..1334)
                     /locus_tag="VW144_001891"
     CDS             complement(495..1334)
                     /locus_tag="VW144_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9601294.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRIGRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1476..2324
                     /locus_tag="VW144_001892"
     CDS             1476..2324
                     /locus_tag="VW144_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9601295.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2417..3334)
                     /locus_tag="VW144_001893"
     CDS             complement(2417..3334)
                     /locus_tag="VW144_001893"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU9601296.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWY"
     gene            3769..3975
                     /locus_tag="VW144_001894"
     CDS             3769..3975
                     /locus_tag="VW144_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1980 domain-containing protein"
                     /protein_id="HEU9601297.1"
                     /translation="MTAFQQLPSSVLQTGLFFSPSVSLDSQTVSAKEYLFPYQKERAQ
                     TTQTSEGTTSQYLKPDSSFYSQLK"
     gene            complement(4286..4579)
                     /gene="rpmA"
                     /locus_tag="VW144_001895"
     CDS             complement(4286..4579)
                     /gene="rpmA"
                     /locus_tag="VW144_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU9601298.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4596..4940)
                     /locus_tag="VW144_001896"
     CDS             complement(4596..4940)
                     /locus_tag="VW144_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEU9601299.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENS
                     SEFVQTRVITEN"
     gene            complement(4956..5270)
                     /gene="rplU"
                     /locus_tag="VW144_001897"
     CDS             complement(4956..5270)
                     /gene="rplU"
                     /locus_tag="VW144_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU9601300.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYIEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5307..5394)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5489..6157)
                     /locus_tag="VW144_001898"
     CDS             complement(5489..6157)
                     /locus_tag="VW144_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEU9601301.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIQNNVSDIRDKLLTLIAFMMGVFLLTIYKEKLRIVKKSILSLIP
                     LAILSIIIGFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSVYLTIFYHEKTILGVPIMMSIFYLS
                     MLFA"
     gene            6559..6858
                     /locus_tag="VW144_001899"
     CDS             6559..6858
                     /locus_tag="VW144_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU9601302.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT         2219 a         1415 c         1158 g         2222 t
ORIGIN      
        1 acaatatttt cggaacattc aactttttaa ctctatttat tactagattt cataattaaa
       61 aagcctactg accaagctag aaagcttgat acaataggct ttttaaagac tgattattta
      121 acagcgtctt taagagcttt accagctttg aatgctggta ctttagaagc tgcaattgtc
      181 atttctttac cagtttgtgg gttgcgacct ttacgttctg cgcgctcacg aacttcaaag
      241 ttaccaaaac cgatcaattg aactttttca ccagctgcaa gatagtcagc tactgctgca
      301 aatacagctt caactgctgc tgctgagtct ttcttagtca attctgtagc ttctgctact
      361 ttagcgatca aatcttgttt gtttgccatg ttaatgattc ctccaaataa tttctaatta
      421 acaaatataa tcatatccta aaatcccttt taggtcaagt taaaaacgct atttcttccc
      481 attttattta ttttttagga gtggtaacgt atcaaaatag cccaagcgtt ctcacccgtg
      541 tgagtttgaa taatggaacc cgtttccaaa acagaaattg gcttttcaac ataagcttgt
      601 aagctttctt tcatctcttt tgcccaatca tcactaccag aatatgaaat tccaatctct
      661 gctacagcac gttcagaaag cgatgttatc aactcatcta accatttttt aaatgtttta
      721 gttccacgac ctttaaccat tggctgcaat tcatggtctt tcatttgcat gacaacacgg
      781 atattgagaa gggagctcaa caagccagtt acacggccaa ttcgtccacc tttgacaaga
      841 ttttccaaag ttgaaacacc aatataaagc tctgtatggt ttttaacctc ttctacatga
      901 gataaaattg cctccatatc tttaccttct tgagctaact tcgcagcctc aacaacttgg
      961 aatttcaggg cttggtcagt gaaggaacta tcaacaacag tcacatctgc agtagatagg
     1021 ctagcacctt ggcgtgctgc ttctaccgta cccgaaagag catgggacat atgaatagca
     1081 agaatctggc caccatcttt gcataggtct tcaaaaatct cagcaaagac acctacaggt
     1141 ggctgacttg ttttcggaag attcttactt tcttgcatca actgaagaaa tttaccttct
     1201 tctttcaaat ccgcatcaga ataaacaaca ttatcaatca ttacagataa tggaacaatt
     1261 gtaatatcta attgctttac tagttcaggt tcaatagtaa cagatgaatc ggttacaatc
     1321 ttaatttttg tcatagtatc aatctttcta ttttaggatt cagattggtt tccttacttc
     1381 taattatatc aaaaaaaaga ttaaaaatcc taatggagtc aatcaaattt tccgtaaaat
     1441 ttgatataat caacttataa gaaaagaggt gtcctatgat taaaaaaatt taccccattt
     1501 ttaccatttt actaggtgct gctatttatg cttttggact gacttatttt gtagttcccc
     1561 atcatctctt tgaaggaggg gcgacaggca ttaccctcat caccttttat ctttttaaaa
     1621 tccctgtttc cctcatgaac ctgctgatta atattcccct tttcatccta gcttggaaga
     1681 tctttggagc caaatccctc tattctagtt tactaggaac cttagctttg tccgcctggt
     1741 tagctttttt tgagcatatt ccccttcata ttgatcttca aggtgattta ctaatcacag
     1801 cccttatagc gggaatccta ttgggaattg gccttggaat tatttttaat gctggaggta
     1861 caactggcgg aactgatatt ctagctcgta ttctcaacaa atacactcat atatccatag
     1921 gaaaactgct ctttatctta gatttttgta ttctcatgtt gattctccta atcttcaagg
     1981 atttgagatt ggtttcctac acgcttttgt ttgattttat tgtttctcgt gttattgatt
     2041 tgattggtga aggaggatat gccggcaaag gctttatgat tatcacaaaa cgtcctgacc
     2101 aacttgctaa ggcgattaat gatgacctcg gaagaggtgt tacttttatt tctggtcaag
     2161 gctactatag taaagaaaat ttgaaaatca tctactgtat tgtcggaaga aatgaaattg
     2221 tgaaaacgaa ggaaatgatt catcgaatcg atcctcaagc ctttataact attacagaag
     2281 cccatgaaat cctaggagaa ggcttcacct ttgaaaaaga ataaaaagag gtaatgtcgt
     2341 gacctcaaaa gttagactaa atcatctatc ttttgggtta cagacaacct cttttttatt
     2401 ttatttactc aagctcttaa taccaattcc gagttacttc ttcatcagcc tttaactgat
     2461 ccactaattg gtcaactgag tcaaatttgg tcatatctcg aatgcgatca agccaataaa
     2521 ccatgacggt ttccccataa atatcttgat taaaatcaaa aatattgact tcaaaacgtg
     2581 cttcttctcc atcaaaggtc acatttttcc cgacactagc catagcacga tacttctgtc
     2641 tttgaatctc aacatcaaca acataaacgc catctgctgg catataagta cggtctaaaa
     2701 gcactaaatt cgctgtcgga taaccaattg tacgaccacg agcattacca tgaaccacca
     2761 tacctcttga tggaagcggt gcccccaaaa gttttcctgc ttctttcaca tttccatcta
     2821 aaatagcttg acggatacga gttgaactaa tctttccttt ctcatcttct acaggtggaa
     2881 caatgataac ttctccatca aagtaatcct ttaaatcttc tgctgttttt ttgtcagaac
     2941 caaatgtata atcaaaacct gcaacaataa ttttggcatt catagccttg atataagttg
     3001 caaagaattc ttgtgcagtg agactagcga attgactact aaaatcaagg agatataatt
     3061 cttctacacc ttcgcgcttt aattttcttt cacgttcagc agggttcaaa atatgcaaaa
     3121 acaaatctgg atgataaggc tctaaagcga tctttggaga ttcattaaag gtcataacga
     3181 cgataggcaa caaatccttt ctcgcagcct tgttggcaac acgaaataat tcttgatgcc
     3241 ccttatgtat gccatcaaaa tagccgagaa caacgactga atcagatggt gtgccaatat
     3301 ctttttggtt ttttatagga atagtaataa tcataaaata attatatcat agcgatagct
     3361 atttctggaa cagaaaatct gaaatgttgt ttttttcaca tgaagtgtac ctgttttcaa
     3421 aaagcacttt attctatcgt tgcttaacta tgaactttgc aatattcttc tcaaaaactt
     3481 gtaggacatc ttcaaaattt tgcaaggagt gattagactt gttcggtaac cataaagtgt
     3541 catactatgc ttatgtatga aaaagcaatg caactaactc ctgagaactt taaattacta
     3601 attggtgccg aaaaggtaga atttagaatc gaggtacacc tatggctgta aaatttacaa
     3661 aatgagacaa cttgggcaag atgtttgaag aatttcctaa actccctgat ttgaagcaag
     3721 tcactttccc tgatgacaaa aaaagccaaa gcagtaaaga aaaactagat gactgctttc
     3781 caacaacttc catctagtgt gcttcagact gggctatttt tctctccatc tgttagcttg
     3841 gattctcaga ccgtttcagc taaagaatat cttttccctt atcagaagga acgggctcaa
     3901 accactcaga caagtgaagg gacgacaagc caatatttga aaccagatag cagtttttat
     3961 agtcaattga aataaaatct gaagaaatcg agtaggaaac tcatatcaat ttttaacagt
     4021 gttctattcc agattcatac tcaatgaaaa ttaaagtgca aactaggaag ttagccgcag
     4081 gctgtacttg agtacggcaa ggtgaaactg acgtggtttg aatttgattt tcgaagagta
     4141 tcaatccact atacatatta aaaagtatgg gtatcaaaac aaaaaaacag gctctccgaa
     4201 aactcggaaa gccttatttt aagctgtttc tagcttcctc acctttacat ttcaaggttc
     4261 gggataaaag gttcaatgga cctttttatt tagcgattgg gtaaacagaa acttgttttt
     4321 tatcgcgtcc tttacgttca aagcgtacta cgccttcaac tttagcgaac aaagtatcgt
     4381 ctccaccacg accaacgttt acacctggat agatgtgtgt accacgttga cggtaaagga
     4441 ttgatccacc tgttacagtt tgtccgtcag ctgctttagc tccaagacgt ttcgcttgtg
     4501 aatcacgtcc gtttgatgta gaacctccac cttttttgtg ggcgaaaagt tgcaagttgt
     4561 taagagtcat ttttaacata atgttttcct ccgtgttagt tttctgtgat aactctggtt
     4621 tggacgaact cagaagagtt ctccgataag tttgccatac ctaagaaaaa tgattcaaag
     4681 aataactggg tcatttctct ctggtgtgaa ggaagatctt ttggtatttc aaccatcaga
     4741 tagccacctt catcttcgtt taattctagg attggttcat agcctgcaaa tttctcaata
     4801 gaattgataa agttaatggc aagcgtagaa accgatgcac acacgacatc taagccgtat
     4861 tcgccactct cggcgtgtcc agtaatttcc gcactcctca gctcgccatc ttcggctctc
     4921 tcaaagactg cttgtatcat gtgttctcct taaaattaag cgttaattgc gttgatgaca
     4981 acttttgtat atggttgacg gtgaccttgt ttacggtggc tacctttttt aggtttgtac
     5041 ttgtaagtaa caactttctt ttgttttcct tgtttttcaa cagttccaac tacagtagct
     5101 ccagcaacaa gtggagttcc gacaacagtg ttttcaccac caacaagaac aacttcgtta
     5161 aaagtaactt cttgaccagc ttcaacgttc aatttttcaa tgtaaactgc ttgaccaact
     5221 tcaactttaa cttgttttcc gccagttttg ataattgcgt atgtgctcat tatgcacctc
     5281 ctatgatttt tatgggtttc cccgattttt ttgtgaagac tcgcctagca tcgtgggaca
     5341 aaccacttaa acttgaataa tcaagcaacg atgttcgtgc ggttgcacag gatcgtgcat
     5401 agtcaactct tcaagtgtag catatttcct attttcttac aagtaataac acctaaaatc
     5461 aagctttttc ttttactttt ttctgccact aggcaaaaag catactgaga taaaaaatgc
     5521 tcatcataat aggaacacca agaatggtct tttcatgata gaaaatcgtc aaatagactg
     5581 aaaagacaac gccaaggaca aaactactaa gcaggctaac aaatatgaac ccttcacgca
     5641 aaaaaggagt gtgcttagtt cggaaataat ctccaaaagc tagcattgtt cgtttgatat
     5701 tccctgtcat aaaggcatta ttgtaggcaa tacccgaaac ctcaccaaaa gcagttgtca
     5761 ccagtcccat acagaaggcc aagggcggca ctagatagat attatctaca gtttgtggca
     5821 caaaaccaat aattattgat aggattgcta aaggaatcaa ggacagaata gattttttca
     5881 caattctcaa tttttcctta taaatcgtta ataaaaagac tcccatcata aacgctatta
     5941 aggttaaaag cttatcccta atatccgaaa cattattttg tatgagctct accgaaagaa
     6001 agacaacatt tccagtttgt ccagctacaa gggtattccc gcgaacaata aaagtgtaag
     6061 catccacata tccagcacaa aacgtcaaaa aaagtgctaa ccttttagac tgacgtgata
     6121 tttttcttat aggtaataac ctcattttac ctcccattgt attttctctt agaaatattg
     6181 taccattttc tttctaaaaa atcgtaggct accatttaga ttttactatt agcataaaaa
     6241 taataataga caactattta tccaaaaata gatatatgta acatgtttgc aaacaaagca
     6301 tacgaacctt tagtaaaatc atttccatga aactagaata gagccctctt agcaaaaatc
     6361 attattttaa tttatttcta atcactcctt gacataaata actctcacca ataaaagact
     6421 atgtcttaaa aaaatggtat aataaaatca atacttgggc ttgatggcta tgctactaat
     6481 aacaattagg agagaaaatc aggcacttgt taacaacaag gattatcccc ttgagatgaa
     6541 aggaacttta gaaatcttat gatgaacatg caaaacatga tgcgtcaagc acaaaaactt
     6601 caaaaacaaa tggaacaaag ccaagctgaa cttgctgcta tgcaatttgt tggcaaatct
     6661 gctcaagatc ttgtccaagc gaccttaact ggcgataaga aagttgtcag cattgatttc
     6721 aatccagctg tcgttgaccc agaggacctt gagactcttt ctgatatgac cgttcaagcc
     6781 atcaactctg ctcttgaaca aatcgatgaa actaccaaga aaaaactggg tgctttcgct
     6841 gggaaattac ctttctaaaa acaaggagct agaacaatgc ttgtcgataa caaaggctaa
     6901 gaaaggtgca aaaatgactc tataatattt gtagtgggta aatccactat agatattatg
     6961 gagcctattt tattgtagaa aaaaagtccc ataagatcta taatgaaaag cgac
//
LOCUS       DAVKJJ010000077         6755 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000077 DAVKJJ010000000
VERSION     DAVKJJ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6755
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.077"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            58..921
                     /locus_tag="VW144_001900"
     CDS             58..921
                     /locus_tag="VW144_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9601303.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDDLEYIYRIEIERS
                     TFLTLEDRTYLEWIKAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEAKELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            1303..1923
                     /locus_tag="VW144_001901"
     CDS             1303..1923
                     /locus_tag="VW144_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601304.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            2009..2452
                     /gene="tsaE"
                     /locus_tag="VW144_001902"
     CDS             2009..2452
                     /gene="tsaE"
                     /locus_tag="VW144_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU9601305.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            2442..2960
                     /locus_tag="VW144_001903"
     CDS             2442..2960
                     /locus_tag="VW144_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9601306.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            2968..3984
                     /locus_tag="VW144_001904"
     CDS             2968..3984
                     /locus_tag="VW144_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU9601307.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSIGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            4069..5325
                     /locus_tag="VW144_001905"
     CDS             4069..5325
                     /locus_tag="VW144_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU9601308.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKTLLSD"
     gene            5380..6546
                     /gene="recA"
                     /locus_tag="VW144_001906"
     CDS             5380..6546
                     /gene="recA"
                     /locus_tag="VW144_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU9601309.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
BASE COUNT         2168 a         1096 c         1507 g         1984 t
ORIGIN      
        1 ttttgaacta aaaattttat aatgcaatta agcaaatagg aaggtttatt atctttaatg
       61 aatacactcg cagagaaatt cagattgaaa agaaaagagt tgagactctc ccaacaaact
      121 cttgcagaag gaatttgtga acaaagtcag attagtaaaa ttgagagagg gcatttcatt
      181 ccctccgcag accttttgtt caaactctct caacgacttg aagtaccatt agattatttt
      241 tttaatgaac aaattgaaat taaatctaac ctctctaatt tcaagcaatt gtctgctcga
      301 ctattagacg atagaaatta tgacgatttg gaatatattt atagaataga gattgaacga
      361 agtacttttc taacactaga agaccgaact taccttgaat ggattaaagc tattattgac
      421 ttttatcaat atgacagtaa gtgtgaagca atttcttcat tggaaaatat attattaaaa
      481 gtctcctcaa atactctgat ttatttaaag gcgttgaata ctctatctaa tttctattcc
      541 ttagtgggtc gtgaacaaga atatgaggca aactactctc atttaataga gttgtatcag
      601 acaaaaaatt tggaccatca agagttttta tttggctaca tcagagttcg ttacaactac
      661 gctcactacc tagtatcaaa ggaaaaatat aatgaagcca tccaagaagc tcttgagacg
      721 attgaactct gtaaacaaag acagacaagc taccaactgg ctcctctact tattcttgta
      781 ggaaatgctg gagccaaatt tctagacaga gaacaagtca aaaattatta tatagaagca
      841 aaagagttat gtaagattta taacaatcct ttaatgctga tgaagataga aaattatttg
      901 aaggaattag atactgttta gttaatcttg acattatagt ttttctgaaa cgttaaataa
      961 cctgtaaggc tgatagtgaa atgaatacta tcagccttat gattatgtga ctattagttc
     1021 gtctcgctcc gttaggtgcg agacaaaaaa acacccgcta gacggatttt catgatttca
     1081 aatttatatt cttcgaaaat ctcttcaaat cacgtcagct ctatctgcaa tctcaaaaca
     1141 gtgttttgag caacctgtgg ctagctacct agtttgatct ttgattttca ttgagtatta
     1201 gtctctagtg tttgtttagc gtcttaaaga gacatttgag aagattagaa acagttctct
     1261 cttttctcct agtataatga ttggtaggaa gggagagaga agatgaaatc aatgagaatc
     1321 ttatttttgt tagctttaat tcaaatcagt ttgagtagct gtttcctatg gaaggaatgc
     1381 atcttgtcct ttaaacaaag tacagctttt ttcatcggaa gcatggtttt cgtttcagga
     1441 atctgtgctg gagtaaatta tctttatacc cgtaagcaag aagtccatag tgtcctagcc
     1501 agtaagaagt cggtgaagct tttttacagt atgttactct taattaattt gttaggagct
     1561 gttcttgttt tgtcagataa cttgttcatc aaaaatacgc tgcagcaaga attagttgac
     1621 tttttattgc catccttctt tttcctattt gggctagatt tgctgatttt tttacccttg
     1681 aaaaaatacg tgcgcgattt tcttgctatg ctagacagaa aaaagacagt gttggtgact
     1741 attttagcaa cacttctttt cttaagaaat ccaatgacca ttgtctcact tctgatttat
     1801 attggactgg gcttgttttt tgcagcctat cttgtcccaa attcggttaa gaaggaagtt
     1861 tccttttatg gtcatatttt ccgagatctt gtattggtca ttgttacgct cattttcttt
     1921 tagagaaagc ctgtttttta tggatgttat gatgataagt ttagtaggaa aatagacaca
     1981 aaagaaaagt ttttggtata ataagagtat gtacacaaaa aatgaagaag agttgcaagc
     2041 cttaggggag cgtttgggcc atctattagc aaagaatgat gttttaatct taactggaga
     2101 actgggtgca ggtaaaacga cctttactaa aggacttgca aaaggattac agatttctca
     2161 aatgattaaa agtcccacct atactatcgt gagagagtat gaaggtcgac ttccacttta
     2221 tcacctagat gtttatcgta ttgaaggaga tgctgattct atcgacttgg atgagtttat
     2281 ctttggtggc ggcgtgactg ttattgagtg gggaaatctc ttaggagatg ccttgccaga
     2341 tgcttattta gaattggaaa ttctaaaaga agcagatgga cgccgtttaa attttcaggc
     2401 aaagggtttg cgtgctgaga aattgttaga ggagcttcaa tatggagtat gaattgctca
     2461 ttagggaagc agagcccaaa gatgcagctg aattagtggc ctttttaaat cgtgtgagtt
     2521 tggagacaga ctttaccagc ctagacggag atggtattct cttgactagt gaggagatgg
     2581 aaatattcct caacaagcaa gctagttcgg acaatcagat aaccttactt gcattcttaa
     2641 atggcaaaat tgctggtatt gtaaatatta cagctgatca gcgtaagaga gtccgtcata
     2701 ttggagatct cttcattgtg attggaaaaa gatattggaa taatggcttg ggaagtttgt
     2761 tgctagaaga agcgatagag tgggcacaag caagtggcat tctgcgtcgt ctccaactga
     2821 ctgtccaaac tcgtaatcaa gcagcagtcc atctttatca aaagcatggc tttgtcattg
     2881 aaggtagcca agagcgtggg gcatatatag aagaagggaa atttatcgat gtttacttga
     2941 tgggtaaact gataggttag tagaaatatg gttaaaaaaa ttattggaat ggtgctagct
     3001 ttactttctg taactgtagt aggagtaggt gtttttgctt atactattta tcaacaaggg
     3061 acagaaacct tagctaaaac ctataaaaaa atcggtgaag aaaccaaggt tattgaagcg
     3121 actgaacctc taaccattct gttaatggga gtggacaccg gaaatgttga acgaactgaa
     3181 acttgggtcg gtagaagtga tagcatgatc ttgatgacag tgaatcctaa aacgaaaaaa
     3241 acaacaatga tgagtttaga gcgggatatt ctgacgcgca ttgaatcagg gaatggtcag
     3301 gctcatgaag cgaaactgaa ctcagcatat gcagatggtg gagcagagct tgctatagaa
     3361 accattcaaa aaatgatgaa tatccatatt gatcgctatg tgatggtcaa tatgagaggg
     3421 ttgcaaaaat tagtggatgc agtaggaggt attacagtca ataatatcct aggtttccca
     3481 atttctatca gtgaccaaga agaatttaat accatttcta tcggtgttgg ggagcaacat
     3541 attgggggag aagaagccct agtctatgca cgaatgcgtt accaagatcc tgagggggat
     3601 tatggtcgtc aaaaacgtca acgtgaagtt attcaaaaag tcatggaaaa agctctcagt
     3661 ttaaatagca ttggtcatta tcaagagatt ctaaaagctt tgagtgacaa tatgcagacc
     3721 aatattgatt tgtctgcaaa aagtatccct aacttgctag gctataaaga ttcatttaaa
     3781 accattgaaa ctcagcagtt gcagggtgaa ggagagatac ttcaaggtgt ttcttaccag
     3841 attgtttcga gagcacatat gttggaaatg caaaatatac tccgacgttc tttgggacaa
     3901 gaagaagtta ctcagcttga aaccaatgcg gttttatttg aagatttatt tggcagagca
     3961 cctgttggtg atgaagataa ttaacttttg atacaaataa aaaaatcaat cgtaggaaaa
     4021 ttcctgcgat tttttcaaaa aaatacgaat agataggtag gaggaaacat gaaagcagaa
     4081 atcattgctg ttggaacaga gattttgaca ggacagattg tcaacaccaa tgcccagttt
     4141 ttgtcggaaa aactagctga gattggggta gatgtatatt ttcagacggc tgtaggagac
     4201 aatgaagttc gtctcttgtc tttgcttgag attgccagtc aacgtagcag tctggtgatt
     4261 ttgacaggcg gtttggggcc aactgaggac gacctaacca aacaaaccct agctaaattt
     4321 ttagggaaag cattagtctt tgatcctcag gctcaggaga agttggatat cttttttgcc
     4381 ctgcgaccag actatgcccg aacaccgaat aacgaaagac aagctcaaat tgtagaagga
     4441 gcgattccac tgccaaacga aacaggactg gctgttggag gaaaattaga agtagacgga
     4501 gtgacctatg tcgtccttcc aggtccgcca agtgaattga aacccatggt cttaaaccaa
     4561 cttctaccca agttgatgac agggagcaag ctgtattccc gagttcttcg tttctttggg
     4621 attggcgaga gccagctggt tacgattttg gctgatttaa ttgataatca gatcgatcct
     4681 accttggccc cttatgccaa gacaggagaa gtcactctac gtctgtcaac aaaggctagc
     4741 agtcaagaag aggcgaatca agcgctggat atcttggaaa atcaaatctt ggactgccag
     4801 actttcgaag gaatttcttt acgagacttt tgctatggtt atggggaaga aactagttta
     4861 gccagcattg tggtagaaga actgaaaagg caagggaaaa ccatcgcggc tgcagagagt
     4921 ttgacggcag gtcttttcca agctaccgtg gcgaattttt ctggagtttc aagtatattt
     4981 aagggtggtt ttgtgaccta tagcttggag gaaaaatcaa ggatgttgga tattcctgcc
     5041 aagaatttgg aagaacatgg tgtggtgtct gaatttacag ctcagaagat ggctgagcag
     5101 gcacgaagca agacccagtc tgattttgga attagtttga ctggagtggc aggaccagat
     5161 agcctagaag gacacccagt tgggacagtc ttcataggct tggcgcaaga tcaaggaact
     5221 gaggttatca aggttaatat tggaggcaga agccgagcag atgtacgtca cattgcggtt
     5281 atgcatgcct ttaacctagt tcgcaagact ttattaagtg actaactttt gatataatag
     5341 tagataggtc tgaggatcat tagaatgtag gagaatagaa tggcgaaaaa accaaaaaaa
     5401 ttagaagaaa tttcaaaaaa atttggggca gaacgtgaaa aggccttgaa tgacgctctt
     5461 aaattgattg agaaagactt tggtaaagga tcaatcatgc gtttgggtga acgtgcggag
     5521 caaaaggtgc aagtgatgag ctcaggttct ttagctcttg acattgccct tggctcaggt
     5581 ggttatccta agggacgtat catcgaaatc tatggcccag agtcatctgg taagacaacg
     5641 gttgcccttc atgcagttgc acaagcgcaa aaagaaggtg ggattgctgc ctttatcgat
     5701 gcggaacatg cccttgatcc agcttatgct gcggcccttg gtgtcaatat tgacgaattg
     5761 ctcttgtctc aaccagactc aggagagcaa ggtcttgaga ttgcgggaaa attgattgac
     5821 tcaggtgcag ttgatcttgt cgtagtcgac tcagttgctg cccttgttcc tcgtgcggaa
     5881 attgatggag atatcggaga tagccatgtt ggtttgcagg ctcgtatgat gagccaggcc
     5941 atgcgtaaac ttggcgcctc tatcaataaa accaaaacaa ttgccatttt tatcaaccaa
     6001 ttgcgtgaaa aagttggagt gatgtttgga aatccagaaa caacaccggg cggacgtgct
     6061 ttgaaattct atgcttcagt ccgcttggat gttcgtggta atacacaaat taagggaact
     6121 ggtgaccaaa aagaaaccaa tgtcggtaaa gaaactaaga ttaaggttgt aaaaaataag
     6181 gtagctccac cgtttaagga agccgtagtt gaaattatgt acggagaagg aatttctaag
     6241 actggtgagc ttttgaagat tgcaagcgat ttggatatta tcaaaaaagc aggggcttgg
     6301 tattcttaca aagatgaaaa aattgggcaa ggttctgaga atgctaagaa atacttggca
     6361 gagcacccag aaatctttga tgaaattgat aagcaagtcc gttctaaatt tggcttgatt
     6421 gatggagaag aagtttcaga acaagatact gaaaacaaaa aagatgagcc aaagaaagaa
     6481 gaagcagtga atgaagaagt tacgcttgac ttaggcgatg aacttgaaat cgaaattgaa
     6541 gaataagctg ttaaagcagt ggaaaaatcc gctacttttt cgatttttga ttcaagtttt
     6601 tagattatat atagtagctt gaaataagat atgaacaact ttattaggaa agtcaaatta
     6661 atttctagaa atgttttagt agctacagcg tactattcca aactcaacca actataatag
     6721 atcgaaacta gaatagtaca catctatttc taaaa
//
LOCUS       DAVKJJ010000078         6325 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000078 DAVKJJ010000000
VERSION     DAVKJJ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.078"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(<1..467)
                     /locus_tag="VW144_001907"
     CDS             complement(<1..467)
                     /locus_tag="VW144_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601310.1"
                     /translation="MPDNTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLNEDYYNILVHEKETIQGYSVLSNCGL"
     gene            complement(457..654)
                     /locus_tag="VW144_001908"
     CDS             complement(457..654)
                     /locus_tag="VW144_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601311.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAR"
     gene            complement(673..1452)
                     /locus_tag="VW144_001909"
     CDS             complement(673..1452)
                     /locus_tag="VW144_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601312.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKKFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGDKANMDVVLVFSKLSDGEKKQLLQARVPFVDFKG
                     NLFFPTLGLVLNANDTEVPKELIPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFKALYLLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENTSYVIWQRKFNQLSLPLS"
     gene            complement(1860..2285)
                     /locus_tag="VW144_001910"
     CDS             complement(1860..2285)
                     /locus_tag="VW144_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601313.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEVASISLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(2414..2773)
                     /locus_tag="VW144_001911"
     CDS             complement(2414..2773)
                     /locus_tag="VW144_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601314.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKETVLG"
     gene            complement(2819..3025)
                     /locus_tag="VW144_001912"
     CDS             complement(2819..3025)
                     /locus_tag="VW144_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601315.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            complement(3012..3173)
                     /locus_tag="VW144_001913"
     CDS             complement(3012..3173)
                     /locus_tag="VW144_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9601316.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            complement(3532..4488)
                     /gene="lytA"
                     /locus_tag="VW144_001914"
     CDS             complement(3532..4488)
                     /gene="lytA"
                     /locus_tag="VW144_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEU9601317.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKYDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIAEKWYYFDVEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(4864..6234)
                     /locus_tag="VW144_001915"
     CDS             complement(4864..6234)
                     /locus_tag="VW144_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9601318.1"
                     /translation="MNKKRTVDLIHGSILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
BASE COUNT         2026 a         1332 c          973 g         1994 t
ORIGIN      
        1 tatagaccac aattactcaa taccgaatac ccctgaatgg tttctttttc atgcaccaat
       61 atattataat aatcttcatt caataataag gctgataaac tagcatcttg gtcaaaatgt
      121 aagggaattt ctcgaccgtc cttctttaat ggatattctg gtaagatact aaatagttca
      181 atcatagaag gaaactcagg attagttgtt gtaaatcgaa aaagctgcgc tttctcatct
      241 ttctgacttc cttgatactc tcccaattct aaaaaatgat tcaaggtagt ataaaattcc
      301 ttatttttta cttcatcaat gatgaccata tcataatctt ttgttgtgcg acttttaaat
      361 ccttgcgaat ccaatacgat agaggtagca gttcccccaa tcagaacata atagttctga
      421 aaatccgcaa acatttcttg aaaaataact ttcgtattat ctggcatcat cttctcccag
      481 atactgtaat atcatatttt ctagtgcttc actctcttcc tctatacgtg ggtcatcatc
      541 atcttttaag gtcaaataaa gagaaatcgg atctacaaat tgtttatcat gattattttt
      601 aaaatcattc caaaactcag atacaaaagg acgatatttc catatctcta gcatctttcc
      661 ttttaaaaca tgctaagaaa gtggcaagga taactgattg aattttctct gccatatgac
      721 atagctagta ttttcatccg tttcagctaa aaaagttgaa tgcgacaaag cataagcacc
      781 accatataga aggttagaaa cagattttat ctgctttata tcgccatctg gcaataaaat
      841 ccgttttttg atgggattaa ataaacatga cacggatttt aagaataatt ctttctttga
      901 caccgtatat gtgtaaagct tattttgctt gtttaacaaa tataaagctt taaaagtcct
      961 caaacaccta taaattgttg agtttggaag tccagtgact tgtgaaagca aatcaacatc
     1021 tactactttt tgaccttttg tcaataaaaa ggcaatccac gttaattgtt cgctaggtat
     1081 taattcctta gggacttcag tatcattcgc attcagtact agtcccaatg tagggaagaa
     1141 gaggtttccc ttaaagtcta caaacggaac tctagcttga agtaattgct ttttttcacc
     1201 gtctgataac ttcgaaaaca ccaaaacaac atccatattg gctttatcac ccatagtgcg
     1261 agcctgagta acaaatgaac tcaaactccc ccttctcttt tctttaatca ataaaaatga
     1321 ttccttatga aaagtcattt gagtatactc aaactttttt acaaaactga ttggtaaagt
     1381 caaagtcagt tcctcaatac tgacatcaac ataaaaggat tgaaaaattg acattatctt
     1441 tttgacattc atgttttttc cttattctat catttttatt atattttatc attcggtata
     1501 ataaaatata agttttttga taaaataacc aaaaactact cgctatcccc tctcacatta
     1561 ccctacatat cgtttgacat gcgactgata gttcaggaaa acttcaagga gaacttttct
     1621 ctcatccact atgcaggact tactatttca cttctactcc tataggctca atttgacact
     1681 tttctttttc gaattgctca tatgcctcct gaatagtgag ttcgatttat ccaaatttat
     1741 ttttgtctat tttgatgaat acttccacga agatagtctg aaaatgaatt tgcaccaatt
     1801 ttccttctct ttttaagcat attattccag atatgattgc cctcttattc agtaataaat
     1861 tacgctttaa acgcttcaat cagtatatct cctgttgtca tatgttctaa agaaaattct
     1921 tctacatatg gtttaacatc tcttaggtta ttacttccta atatcccatt atgctttttc
     1981 gctaaggaaa tagaagccac ttctccctta ccaataatct tgttactatc atgatttctt
     2041 gttaaatctc tatataatgc gtattcttca gttccaatgt ctatgctcac aatctcagct
     2101 gaacccttag ttaccaactg gtctatccta gattttaaat ggggaattgt aggtatattg
     2161 atttcatcat acacctcttg tggaataaca attttacccg aatagagctt ttctaaaaga
     2221 tgttcagtgc caacccataa aaatactgaa atacaatctg catcaataaa tacacgacta
     2281 gtcaaacgac aactcccccc tcttcatata gcccatatac aatatcatat ctgaaagcat
     2341 ctagtaacag ttcctcatac ttcccttgcg aaattctgtt attttctaaa agttgttcag
     2401 tcgaattaat atattatcct aataccgttt cctttttact ttctgacaaa ggacgatata
     2461 aacttgtatc atagcctaat cttgaagctg tctctataac actaatatcc atatttttaa
     2521 tttcttctgc atcaaggtat ccatcattcc tcaatctata taacatagct ttatgactga
     2581 taccataaaa ctgacccaat tttataatat cttctacttc aagatgagtt ctattggcat
     2641 tttctctgat ttcctcaacc atcctataca gtgaagatgg gaaaattaaa aaataagaag
     2701 caaactgatc cgcttttctt tcagtttcat ctccttcacc aatcaagata agactgactg
     2761 aactcttctt cacctcatca taataaagat gatacagtcc atgtgctaaa gaaaatcttt
     2821 accttcctaa tggcatgtct gaattgactg caatgagact gaactgagtt cctttataac
     2881 agactccgct aatattcttt ccgagtccat aaaataccag cgtcaaattt tctatctttt
     2941 gtaccaattt aaaaatatct atcggcgatt caccatcagc tcccaacttt tttctaagat
     3001 ttgaagcttt attacttaaa tccattcgtg atattccttt cacctttttc cattttggca
     3061 atcatgcttt gattcaaaga caaatactca gcaatcttat cttgagtaag atttgaagat
     3121 attctaagtt cttttattct ttttccaaca tcacatacat ttatcacata catttatcat
     3181 attcatacac ctctcataaa aagaatagca cactcttgtc ataatttttt aaataaaaaa
     3241 attatgacaa aacaaggaag caatttattg atgctgctta aaaatctaaa attgatgagt
     3301 tcctatcaaa aatcgtatct tcaacctcaa aacagtactt aaagctatcc gactcggttt
     3361 acattgtcaa atttagattt tatttgagca taactttcta gtttgctttc tgatttttgt
     3421 ttaatatagt agcaaacatt gaaggaaata tctccacaaa aaaacgcata ctattaagct
     3481 ttttcaagac ctaataatat gcgctgttct gatttgaaag acattccatt attattttac
     3541 tgtaatcaag ccatctggct ctactgtgaa ttctggcttg tctgccagtg ttccgtctgg
     3601 tttgaggtag taccagcctg ttccgtccgc tgactggata aaggcatttg ataccatggc
     3661 gccttcttta gcgtctaagt agtaccaagt gtccttgtac ttgacccagc ctgtcttcat
     3721 ggcaccttct acatcaaaat agtaccactt ctcagcgatt ttcttccagc ctgtagccat
     3781 ttcgcctgag ttgtcgaacc agtaccagtt gccgtctgtg tgcttcctcc agcggtctgc
     3841 aagcatatag cctgaactgt caaagtagta ccaagtgcca ttgattttct caaacttgtc
     3901 ttttggataa gagccgtctg aatgtacgta ccagtagcca gtgtcattct tctgccagcc
     3961 tgtttcaatc gtcaagccgt tctcaatatc atacttaaac tgctcacggc taatgcccca
     4021 ttttgccaag taagggtatg gatccacatg gtctgagtgg ttgtttggtt ggttattcgt
     4081 gcaatactcg tgcgttttaa ttccagctaa actccctgta tcaagcgttt tcggcaaacc
     4141 tgcttcatct gctagattgc gtaagagttc gatataaagg cggtagtccg tcatgaactc
     4201 ttctttagtt gaatggcttt caatcagttc aaccgctgca taggtctcag cattccaacc
     4261 gcccccaacg tcccaggcac cattatcaac aggtcctacc tgcatgatgc aaccgttccc
     4321 aacaatgtgc gagaaaaaac ctaattctgg gtctttccgc cagtgataat ccgcttcatt
     4381 ctgtacggtt gaatgcggat tcccagttga gtgtgcgtgt acttgcctat atggttgcac
     4441 gccgacttga ggcaaatctg ttcttaattt actcacatta atttccatat tctactcctt
     4501 atcaattaaa acaactcatt ttttacaatc cagaaccaga aactccttta tttctacctt
     4561 acaaagaaga caatcttagt cacgattagg cttgtagata gaacctcaaa acgcactatt
     4621 ttgacactgt aaataggact gacaaggtct gcattctatc tacaataaca ccccagacta
     4681 aaaagctttt caaaagtata tttttacagt ctctatatgt ccttttcata aatactatac
     4741 tttattatat catataaaag aagtcaaaag tctgttaaac tattttcaac accaaactaa
     4801 agaagagaac acaagttttt tcgatgttca ctagaggaaa tggattttat tcagtaaatc
     4861 caactaggat tgcactttgg ttgccaaaat tgcctttcct tcttttatca agggatgacg
     4921 gaatagtgag aagtacagtt gaactgtcat ggcaacccag ataagaggtt cacaaaggat
     4981 aacaccctta tatcctgccc aaggaataat caaaaccaca aaaacgattt ttccgattag
     5041 ttcaataaag ctagaaacta gaggaaggat cttctgcccc aagccctgca agcaattgcg
     5101 ataaatcaac aagaggctca aaatgggata aaaggctgaa ctgatttgca gatagagact
     5161 tccattttct atcaagtaac catctgtcga actagccaag aaggaaacca aggctggact
     5221 ggcaaaaaag aggaaaatac aaacaaaaac tgcccaggat atacttaaac gactgccgat
     5281 tcgaagacct tgaacaatgc ggtcaggtcg cttagctcct agattctgag aagcaaaggt
     5341 cgtcattgat gcagaaatag cggtcatagg aagaagggca aaggtcataa tgcgtcgagc
     5401 tgccgtctgg gcactaataa tcactgcacc aaatgtatta acagaaaact gtaaaatcac
     5461 actgccgata gatacaattg aactcatcaa gcccatagcc aaaccttgct ccaagagatc
     5521 cgcgtacaag cttttgtccc atttgaaatg tttaaactgt ggcaagagtt ctggcacact
     5581 tttacgaata taataaaagc agagaaccgc tgataaacct tgcgaaataa tggtagcaag
     5641 tcctgcggat tgaactccca gatgcaattg cgtaataaaa tagagatcca gaaccacatt
     5701 aaccaaggca gagaaaatca gaaatcccag ggctgctaga ctgtcaccaa tagaccgcaa
     5761 caagcctgca aaaagattat aagcaaagct gacacctaca caggtcacaa tcatagaaat
     5821 atattgataa gattgaggaa gaatttctgc aggagtatct aagtattgca agagaggata
     5881 caagccaaga aagcccagca acataactag aatgctcaaa agagcaccta aaatccaggt
     5941 ggctgctact gcttccttga ttttagtgaa attccgagcc ccataataac gagcaatgac
     6001 aatccccatg ccattgccaa caccaagtgt aaaacctaca atcaggtcaa aaatcgctgt
     6061 cgtcgctcct actgcagcca aggattcttg accaagaaat cgtccaacaa tcaagacatc
     6121 agcagtgtta tagagctgtt gaaaaatatt tgatagcaag attggaaagg tgaagcttaa
     6181 gagcgaggga agaatcgaac catgtatcag gtccactgtt cgttttttat tcataaagct
     6241 attatatcag ctttccagag gagcgacaag aaacagcaaa cttatatata gcggaatgaa
     6301 ataaggacag tcaaatcgat tttta
//
LOCUS       DAVKJJ010000079         6269 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000079 DAVKJJ010000000
VERSION     DAVKJJ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.079"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            1..1691
                     /locus_tag="VW144_001916"
                     /pseudo
     CDS             1..1691
                     /locus_tag="VW144_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268095.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            1991..2830
                     /locus_tag="VW144_001917"
     CDS             1991..2830
                     /locus_tag="VW144_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU9601319.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKIGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGVIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            2848..4686
                     /locus_tag="VW144_001918"
     CDS             2848..4686
                     /locus_tag="VW144_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9601320.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFELDSVNQGYKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGVPVTPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPAVYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDAPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVEAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            4699..6114
                     /locus_tag="VW144_001919"
     CDS             4699..6114
                     /locus_tag="VW144_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9601321.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLLGIDFYHKYKEDITLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
BASE COUNT         2098 a          827 c         1292 g         2052 t
ORIGIN      
        1 ttaaagcagg ctatgcgaca gccaacctat caaattcctg agcgaatgta tttatttgga
       61 gaatccgatt attatttatg gctgccaaga ggcttgttag atccattgca agataaattt
      121 aagcaggtag ttgtggagga taggagaaaa gtacaaaggt ctattagagt agcatttaag
      181 ggagagctta ctttggagca agaattagcc ctgtcagata tgaattctaa agaaaatggt
      241 ttacttcatg cgggacaggt tttgggaaga ccgttttagg tgctgctctt atctctgaac
      301 ggaaaacaaa aacaattatt ctagtccata ataagcaact cttagaccaa tggctagatc
      361 gcttaaactg ctttttgact ttcgaagagg aggaggctat ccgttatacg gcatcaggtc
      421 gtgaaaaggt aatcggctat gttggacagt acggtgggac taagaaatgg ctgagtaaac
      481 tggttgatgt cgttatgatt caatctctat ttaagttgga aaatagtcaa agtcttttgg
      541 atgagtatga gatgatgatt gtggatgagt gtcatcatgt ctctgccttg atgtttgaaa
      601 aagttgttgc tcagtttaga gggaagtatc tttacggttt gacggctacg cctgagcgta
      661 agaatggtca tgagcctatt gtttttcaga gaattggtga gatactccat actgctgata
      721 agagggaaac ggattttaaa cggcaattgc aattaagatc cacttctttt ggtcatttgg
      781 agattgaaaa gaccaaagca agtaatttta tacagcttag taattggatt gctactgact
      841 cagcgaggaa tcagttgatt ctcaaggata ttctagccca agtggcagaa ggacggaata
      901 tcttggtttt agttaatcga attcaacaga tagatgtctt tgaaaaatta ttgaaagaga
      961 aagaggttga tgactgttac attattagcg gaaaaaccaa agtccgagag agaacgagtt
     1021 tactggagac gttagaacag ttagataaag ggtttgtttt gttgtctact ggaaaataca
     1081 ttggcgaagg ttttgactta cctcagttgg acacgcttat cttggcagca cccttttctt
     1141 ggaaaaataa tttgattcag tatgcaggtc ggattcatag aaactacaag gataagtctt
     1201 tggtgcggat tttcgattat gtggatattc atgttcctta tttagaaaag atgtttcaga
     1261 aacgacaagt agcttatcga aagatggatt atcgtgccat tgagggtgag gagaaacaat
     1321 tcgtttatgt tgatagtaga tatgagaagg tgttgagaga ggacttagca ggggaaagac
     1381 aggaatgtat gcttatttta ccttatgtgc accagacaaa actgatgaat tttctaaaag
     1441 aatttaggat tagtcaaatt gagatatgta taccagagac ggttgtaaat aaagcattgc
     1501 tagatcagtt gaagagccag aaaattaaag tgtcttttac tcaatcaaaa atagtaacgc
     1561 ctattctttt ggtgaataag actattgttt ggtatggtgc aatgccatta ttagggaagg
     1621 tagatgagat gaccatatta cgtttggaat tagctagtat agtttctgaa ctagtggcag
     1681 gtttacgata gagaaaattt ttaaaaattt ctatgtatga ttttcatttc tttagtgaga
     1741 ctgttgccat tatcacattc gaatcacaca aaataaaaaa atttctacaa gtacttgaca
     1801 aatagattga aatatcttaa aatgaaaatg gttacagagt tattaattat ttaagcttca
     1861 tgtcaccatt aaaaattgaa ataaaaggat gttatcacta atacaagtga gcaggaacct
     1921 atttaatcac atcagaagaa gtttcttgat gtttttaagt aggttccttt tattttaaaa
     1981 gggaaatttt atgatcataa aacgaatact aaaccacaat gccgtaattg cgcaaagtaa
     2041 aaaagatatc gatattcttc tttttggaag gggaatagct tttggaagaa aaattggaga
     2101 taaagtaaat ccaattgata ttgagaaaag tttttttctc aaaaatagag ataatatgac
     2161 ccgttttaca gagatgttta ttaacgttcc tttggagttg gtgtacatca ccgaaaaaat
     2221 aattaaccta ggtaaaataa cattgggtaa taattttgat gaaattatct atattaattt
     2281 aacggatcat atttcttcga gcatagaacg ttataaagaa ggagttatta tttcgaatcc
     2341 cctacgctgg gaaatatcga aatattataa agaagaattt gaacttggga aaagggcttt
     2401 acaaataata aaaaaagagt taggtattga acttccaatt gacgaagctg cattcatagc
     2461 gctacatttt gttaatgcta atttagaaaa taattttcaa gagtcgtata aaatcactga
     2521 aataattatg ggaattgaga aaatcattca agatttctat tgtactgagt ttaaccaaga
     2581 ttctattgat tattatagat tcataactca tataaaatta tttgcccatc gcttggttga
     2641 aaatacaact tattgtgacg atgatgatga agacttgtta gcattaatga aaaataaata
     2701 tcctagagaa tatgaatgtg gtgaacaggt ggcaatgttt atacaaactg agtacaatta
     2761 tttgctgact tctagtgaat tagtttattt gatggctcat attcgtcgct taacaaaaaa
     2821 tttagattaa tagaatagga gaataccatg agttataaag atacggtaca aaaaatcctc
     2881 gatgtaattg gaggtgaaaa aaatgtcaat agagttaccc attgtgtaac acgtttaaga
     2941 ttagaattaa aagatgaaaa tttagtcaat gatgatgatg tgaagaagat accaggtgta
     3001 ataggtatta tgaaaaaaaa tggacaatat caaattatac ttggtaatga tgtagctaat
     3061 tattataaag aattcgttaa acttggcaat tttgaattag attcagttaa tcaagggtac
     3121 aaagggaata ttttagaaag aatcattgag tatatcgctg gttccatgac tccaatcatt
     3181 ccagcaatgt tagggggagg tatgctgaaa gtcttggtaa ttattttacc aatgcttggt
     3241 atattgcaat cagattctca gactattgct tttttgacat tttttgggga tgcgccatat
     3301 tatttcttac cgctgttatt agcttattct gcatcacaaa aattaaaagt aacatctaca
     3361 ttagctatgt ctgtagcagg tgtacttctc catccaaatt ttgttcaaat ggtgcaatca
     3421 gggaatcctc ttagtttatt tggtgtacct gtgacaccag ctagttatgg ttcgtcagtc
     3481 gttccaattc ttattatggt ttggttgatg aaatatattg aaaaaatgat tgctaaatta
     3541 acaccagctg ttactaagag ttttttgcaa cctacgctag tattattagt atcaagctgt
     3601 attgccttag ttgtagtcgg acctattgga gtaattgttg gtgaaggatt atcaaatcta
     3661 gttgggcaaa tgtatggtgt agctggatgg cttacattag ctattcttgg tgctattatg
     3721 ccatttattg ttatgactgg aatgcattgg gcttttgcac ctattttttt ggcggcatct
     3781 attgctactc cagacgtatt aattcttcca gcaatgttag ggtcaaactt agctcaaggg
     3841 gctgcttcga tggctgttgc attaaagagt aaaaataata atacaaaaca aattgctttt
     3901 gcagcaggtt tctcagcctt acttgcaggg attaccgaac ctgcagtata tggtgtgact
     3961 ttaaaatata aaaaaccgct ttatgcagct atgattggtg gtggattagc gggattattt
     4021 gcaggtctta ctagtgttaa agcgtatcta tttgctgtcc catctttgat agcgttgcct
     4081 caatttattt attctgatgc accatcaaat attgtaaatg ctttaattgt ggcggtcatt
     4141 tcggttgtta ttacctttgt attagcttat atatttggaa tcgatgaaga agagagttct
     4201 agtaatttag aagttgaagc tggagtttca aataaaaaaa tgatattttc tcctatatca
     4261 ggagaaatca ttccgttaag cgatgttcag gataaaacat tttcagataa actaattgga
     4321 gacggagtag cgattatccc aagtgaaggt aaggtttatg caccatttga tgggaaaatt
     4381 acaaatattt ttccgactaa gcacgcaatt ggattgaaga gtgatgaggg tgttgagtta
     4441 ctaattcata tcggattaga tactgttgag ctaaaaggtc aaggttttat tagtcatgta
     4501 gaagaaggag acagagtttt caaaaatcag ttgatttttg aaatggactt aaatttaatc
     4561 aagacgaaag gctacgaaac agttacacca gtaattgtaa cgaataccaa tgattttcta
     4621 gatgtattag tattacctaa taatcagaca atcgagcatt ctaaggaact actggtaata
     4681 ttataatgga ggaaagttat gaccattttt ccagatgatt ttctttgggg tggagctgtt
     4741 gcagctaatc aagtagaggg agcatataat gaagatggta agggcttatc agttcaggat
     4801 gtgttaccca aaggtggatt aggagaagca acagaaaatc ctacagaaga taacttaaaa
     4861 ttattaggta ttgattttta tcataaatat aaggaagata taaccttgtt ttctgaaatg
     4921 ggctttaatg tttttcgtac ttctattgca tggagtagaa tttttccaaa aggagatgaa
     4981 gaagaaccta acgaagctgg tttgaaatat tatgatgaat tgtttgatga actacatgct
     5041 cacgggatag agccacttgt aactctttca cactatgaga ctccattata tttagcaaga
     5101 aaatatcatg gatggattga taggagaatg attcattttt atgaaaaatt tgctcgaaca
     5161 gttttagaaa ggtataaaga taaagttaaa tattggctta catttaacga agtaaactct
     5221 gttttggaat taccgtttac tagtggagga atagatatac ctaaggagaa tctttcgaaa
     5281 caagaattat atcaagctat acatcatgaa ttagtcgcct cgagtttggt tacaaaaatt
     5341 gctcgtgaga ttaattcaga gtttaaggtg gggtgtatgg tattagctat gccagcttat
     5401 ccaatgactc caaatccaaa agatgtatgg gcgactcatg agtatgagaa tctaaattat
     5461 ctattttcag atgtgcatgt tagaggttat tatccgaatt atgcaaaaag atattttaag
     5521 gaaaatgaca ttaacataga gtttgcagct gaagatgcag agttattaaa aaattatact
     5581 gtagattttt tatcctttag ctattacatg agtgtgactc aatctgctct tccaacacag
     5641 tataattcag gagaagggaa tattattggt ggtttagtaa atccttattt agagtcttca
     5701 gagtggggat ggcaaattga tccaattgga ctacgtataa ttttaaatag atattatgac
     5761 cgttatcaaa tcccattatt tatagtagaa aatggattag gtgctaaaga tcaactaata
     5821 aaagatgaac ttaataactt aaccgtccaa gatgattata gaattcagta tatgaaagaa
     5881 catttattgc aagttgctga agctctacag gatggtgttg aaattatggg ctatacgtct
     5941 tggggatgta ttgactgtgt ttctatgtcc actgcacaac tttctaaaag gtacggtctc
     6001 atttatgttg atcgaaatga tgatggaaat ggcacattca atcgatataa gaaaatgtcc
     6061 tttacttggt ataaaggagt gattgaatca aatggagaat ccttattcaa atagtttgtt
     6121 tattgatgat tagatattat cacggttaag gcagcatctc attaaatatg aaataaagag
     6181 gtttggaaga catcaaattc taaacctctt tattttatag ggggattaat ttaattttgt
     6241 tacagagagc cgattataaa tagttaact
//
LOCUS       DAVKJJ010000080         5958 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000080 DAVKJJ010000000
VERSION     DAVKJJ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.080"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            19..1641
                     /locus_tag="VW144_001920"
     CDS             19..1641
                     /locus_tag="VW144_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9601322.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLIVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            1703..4255
                     /locus_tag="VW144_001921"
     CDS             1703..4255
                     /locus_tag="VW144_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU9601323.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(4285..5916)
                     /locus_tag="VW144_001922"
                     /pseudo
     CDS             complement(4285..5916)
                     /locus_tag="VW144_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
BASE COUNT         1876 a         1101 c          985 g         1996 t
ORIGIN      
        1 acatatagga gaaaaatatt gcttacagta tctgatgttt cactacgttt tagtgatcgc
       61 aaactttttg atgatgtcaa tatcaaattt acagaaggaa atacttacgg attaatcggt
      121 gctaatggtg ccggaaaatc aaccttttta aaaattttag ctggagatat cgaacctact
      181 actggtcaca tctctcttgg tccagatgaa cgtctctctg ttcttcgtca aaatcacttt
      241 gactacgaag atgaacgtgc cattgatgtc gttatcatgg gaaatgaaaa actttatagc
      301 atcatgaaag agaaagatgc tatctacatg aaggaagatt tctcagacga ggacggggtt
      361 cgtgctgccg aactcgaagg agagtttgcc gaacttggag gttgggaagc agagagtgaa
      421 gcctctcaac tacttcaaaa cctaaacatt ccagaagaat tacactacca aaacatgagc
      481 gaattggcca acggtgaaaa agtaaaggtt ctcctcgcca aagcactttt tggtaaacca
      541 gatgttcttc tcttggacga gcctactaac ggtttggata tccaatcgat tacttggtta
      601 gaagacttct taattgactt tgataacaca gttatcgtag tatcccacga ccgtcacttc
      661 ttaaacaaag tttgtactca catggccgac cttgactttg gaaaaatcaa actctatgtc
      721 ggaaactacg acttctggaa ggaatcttct gagcttgctg ctaaattgct agcagaccgt
      781 aatgctaaag cagaagaaaa aattaaacaa ttgcaagaat tcgttgctcg tttctctgcc
      841 aatgcttcta agtcaaggca ggcaacatca cgtaagaaaa tgcttgataa gattgagcta
      901 gaagagattg tgccatctag tcgtaaatat ccatttatca actttaaagc ggaacgtgag
      961 attggtaatg atctcttgat agtagaaaat ctaactgtaa agattgatgg tgaaactatc
     1021 ttggataata ttagtttcat cttgcgtcca gatgataaga cagcacttat tggacaaaat
     1081 gatattcaaa cgactgcatt aattcgtgca atcatgggag atattgacta tgaaggaact
     1141 gtcaagtggg gagttacaac tagtcaatct tacctaccaa aagataactc agctgatttt
     1201 gcaggaggag aatcaattct tgactggttg cgtcaattcg caagtaaaga agaagatgac
     1261 aatactttcc tacgtggctt cctcggccgt atgctcttct ctggagatga agttaacaaa
     1321 cctgtaaatg tcttgtcagg gggagaaaaa gttcgtgtca tgctttcaaa actcatgctc
     1381 ttaaaatcaa atgtccttgt acttgatgat ccaacaaatc acttggactt ggaatctatc
     1441 tcaagcttga atgatggatt gaaaaacttt aaagaatcaa tcatctttgc tagccatgac
     1501 cacgaattta ttcaaacttt ggctaaccat atcattgtct tatctaaaaa tggcgtcatt
     1561 gaccgtatcg atgaaaccta cgatgaattc ctagaaaatg cagaagtaca agcaaaagtt
     1621 aaagaacttt ggaaagacta aataaaactt caaaactcag ttgggttaac caactgagtt
     1681 ttctatcatt ctacgaggta acatgaaatc atttttaaaa acatatcgaa cctattttat
     1741 ttctttcatc attcctgtag tgattatgtc tggagtatat ctatctcaag gtatctactg
     1801 gaatagcgac aactctcctc tattaggaga tggctttcat caatacgtta tttttgatgt
     1861 agccttacga aatatcctac atggaaatag tagtctgttt tacaccttta caagtggtct
     1921 agggctaaac ttctatgccc tatctagtta ttacttgggt agttttctcg cgcctctagt
     1981 ttactttttt gatctaacga atatgccaga tgctgtctat ctgaccactc tcttaaaatt
     2041 tggattgatt ggtctgtcaa ccttttttag tttgaataaa ttgtttcaat ctatccctca
     2101 gactttaaaa ctagccttat ctacttccta tgctctgatg agtttcactg tcagtcaatt
     2161 agagataaaa acctggctag atgtttttat cttgattcct ttaattataa ctggtttaca
     2221 tctactgata actgaaaaga aactcctatt gtactttaca agtctgtcaa tcttatttat
     2281 tcaaaattat tattttggat atatgacagt attgtttctt attttctggt atctctgtca
     2341 aatttcgtgg gactttaaga ctcgaaaatc atctgttctt gatttcatag ttatctcctt
     2401 tttagctggt atggctagtt tgattatgac tcttcccact ctatttgatt tacagacaca
     2461 tggggaaaaa ttgactgaag ttacaaagtt tcaaactgaa agtagctggt atcttgatct
     2521 ctttgctaag caattcattg gttcctttga cacaacaaag tatggggcca tcccaatgat
     2581 ttttgttgga ctatttccct ttattttgac cattttattt tttacgctga aatctattaa
     2641 gtttcacgtg aaactcatat atgtaatatt ctttgcattt ctaattgcaa gcttttacat
     2701 agaagctctt gacttatttt ggcaaggcat gcatactcca aacatgtttt tacatcgcta
     2761 tgcttggatt ttctctacct tgttaattta cacagcagca gaagtcttaa agcgtctgaa
     2821 agaacttaaa gtctggaatt ttttagtttc gctttttctt gtagtagcag gatttttagc
     2881 taccatctat ctaaaatcgc attattcttt tttaacagat ttgaatattc tgcttactct
     2941 tgaatttttg gttgtctatt ctcttttact ccttgcagtt atcaaaaagt ttatatctgt
     3001 gaatctattt gccattctaa tctctttatt tatactggtt gaaatgagtt taaatgcttc
     3061 atctcaaatg gacggaattg ctaaggaatg gggatttgct tctcgaagtg cttatagtcg
     3121 agatatccca gctatggaat ctttctcaac atatattgga aatcaattta ctcgtactga
     3181 aaaactacaa actcagacag gaaatgacag tatgaaattc aactacaatg gaatctctca
     3241 attttcatct gttcgaaatc gttcatcaag ctctacttta gataaacttg gttttaaatc
     3301 ctctgggact aatctcaatc tccgatatgc aaataatagt attttggctg atagtttatt
     3361 tggtatccag tacaatatct cagacagtcc tattgataag tatggcttta aagatatcta
     3421 tcaaaaagat aatcttaccc tatatgaaaa tcaatactct cttccgattg cagttgctag
     3481 tcaatctgtt tacaatgatg tcaagttcaa tgaacatacc ttggataatc aggcctcatt
     3541 tttaaatcaa cttgctaacg tcaattttga ttatttttct ccaatacctt atgaaaaaac
     3601 agaaaaaata gaaaatacta atgatttgat tagtgtcaca agttcttcaa atgaagatgc
     3661 agcaatccag tatcaaattg aagttccaga aaacagccaa gtttatctct ctttcataaa
     3721 ccttcacttt tctaacgata aacaaaagaa ggttgacata cttgtaaatg gtgaaaaaaa
     3781 gacttttaca actgataatg tcttctcctt ctttaatcta ggatatacta aagagaaaaa
     3841 aactttcaat atcaatgtta gtttccctgg aaattcacaa gtatcatttg aatctcctac
     3901 cttctatcgt ttagatacca aaactttcac cgaggcaatt caaaaaatta aagaacaacc
     3961 tgtcacagta tcaacttcta aaaacaaggt ttttgctaca tatgatgtcc aacaagatac
     4021 atctattttc ttcaccattc cttatgacaa aggttggtct gcctaccaag atggtaagaa
     4081 aatagaaatt aaacaagctc aaactggatt tatgaaagtt gacattccca aggggaaagg
     4141 aactattaca ctttccttca ttcccaatgg ttttattact ggagcaatct gttcctttac
     4201 ttctctctta ctatttggaa tctataatca cagacgaaag tcatctaagg cataaaaaat
     4261 cgaccagtta atcggtcgat ttttttaatc ttcttccatt ttcttatgtt gataggctag
     4321 catacctaaa ccagtaaata gagctagtat gacagctagg aaaatgactt gttgatagag
     4381 attgtttgtc gtaatcccga aactgaatag ctcattggta accagggatt aatagatcta
     4441 aagaaatcat ttgtcaaagc aagtggataa gtacctgcac ttgatgctaa ctgtagtaaa
     4501 agcaaaataa gtgagaaaaa agctcctata cggctattcc acgttgctaa agtggtcacc
     4561 atagacatga atactaaact tgttaggatg atgagaataa atattctcat ctcatgatta
     4621 gcagttaaac caataagctg aactcctcca tataccaaaa ttcctgccaa aacagctata
     4681 ataccattta tttcagctca agatttcaac caagcccaac ggctctctgg atgacgtcct
     4741 gaaggcaatt tcgcaaatat catatttgtt gatattgctg caaaaaaaag agcaactgat
     4801 atcatataag gagctattgc gattccattt acaggaactt gatcattgtc tgtttttgaa
     4861 agattgagtg gatttgacaa aatctctgca tttttagatt ctgttgatac tgatttgagt
     4921 tgatcactag cattacctag tccttgtcct aaagaagcaa gtcctgtctg taaatcttcc
     4981 aatccagaag ttaactttgt tccaccttct gctagtttcc cagatccatc tgccaattta
     5041 ttagctctat tctctaatgg agaagcacct gaaagtaact gactggattt gtctgctaat
     5101 tgcccagatc ttgctgtcaa tctagaagat ttttgtgtca aggtgtttga gcctgaaact
     5161 agtttatcca aactacctgt caaggtggca tttttttcac ttagttgact tgcgccctga
     5221 gaaactttat caacacctgt agtatatgcg tttacacctg atgcaatcaa ctgactggca
     5281 ggaactaatt tgctagtaac agctccttgt atctctgtta atccacttga caatcctatc
     5341 aaagaagtag aagtaatagg taatacttga ttagcttgat tttttaaagt cgaaagatta
     5401 gaagattgat tttgtaagtt ttctaaactt ccctgtaaac cttgtactaa agctacaatt
     5461 gactgagccg attgaatact atcagttgaa ttttgagata cagaatcact tatcccagtt
     5521 tgttgctcac ttgtcaatga ttgataagct gctgtcgatt gaatattgga taatgtagtt
     5581 gtttttttag ataaatcact tggtaaagtc actaccatat agtaatcgcc atcttccaat
     5641 cccttctttc cttcctcttc atctacaaaa tgaaaatcca aggttttatt ttcttttaaa
     5701 ttggacacca tgtcttttcc tattgccata gtattaccat tataggaagc ctctttatca
     5761 ttatttacaa ctgccacagg taagtcagac aattgcccat atggatccca cattgatgac
     5821 aaaaatatga tattgtacag agctggaata agagaaatcc cgatcatgac aataataaag
     5881 gttgtttttt taaattgctt tccattcttt aaacatagtt tctccttttt taaacatatt
     5941 gtctaaaatt ttgatata
//
LOCUS       DAVKJJ010000081         5622 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000081 DAVKJJ010000000
VERSION     DAVKJJ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5622
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.081"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            37..984
                     /locus_tag="VW144_001923"
     CDS             37..984
                     /locus_tag="VW144_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEU9601324.1"
                     /translation="MTEEKFQKIIQSKDTETLAFILESTPYHLSIDILEDPSQTEISL
                     MTKLVNDYRWAYAESPSDIIVTLFALRYVYHNIKVLLKSKAAIKKDFSKLLIPIGIFD
                     IESLKHLVSSLHSDTLPDFMVREVESIWNEYETFNNIRVLDVGADLAYFKHLKLLSNE
                     LDEVLSQVIVEMIDFYNIITVKRGLSQNKSHGDILQLLSDEGSISAKEFIYIVENQEI
                     FVWFNKINPSLDSIFSTYELKMQDATISSSELEFLCDLLLYKTLDQGRYNVEGPLVLA
                     RYLLGCEFEVKNLRMIISALQNTIPFESIKERIRPHYGS"
     gene            974..1294
                     /locus_tag="VW144_001924"
     CDS             974..1294
                     /locus_tag="VW144_001924"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEU9601325.1"
                     /translation="MEANKYKIGIIGSRDIILPFSMIGFDIFPAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAVG
                     HNIL"
     gene            1357..3132
                     /locus_tag="VW144_001925"
     CDS             1357..3132
                     /locus_tag="VW144_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEU9601326.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTISIGQKVSTGDILGTVKETEVVNHKIMVPYGISGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQALGLPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSAGTYIDDKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSENERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIIEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIQLILETGGL"
     gene            3133..4518
                     /locus_tag="VW144_001926"
     CDS             3133..4518
                     /locus_tag="VW144_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEU9601327.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLPSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVKFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            4541..5152
                     /locus_tag="VW144_001927"
     CDS             4541..5152
                     /locus_tag="VW144_001927"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEU9601328.1"
                     /translation="MVRLNVKPTRMELNNLKERLKTAERGHKLLKDKRDELMRRFISL
                     IRENNQLRKEVESYLIDNLKAFAVAKSLKNSLMVEELFSIPSKEIELFVEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIDKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIMKVK"
     gene            5228..5431
                     /locus_tag="VW144_001928"
     CDS             5228..5431
                     /locus_tag="VW144_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9601329.1"
                     /translation="MTVSGYLFQNFGLLDNENVSTDLDLGLISRKCNFKEKDKLKKDV
                     LARVGLTHIHLEQKIYELSGGEA"
BASE COUNT         1888 a          810 c         1140 g         1784 t
ORIGIN      
        1 ataaatacga cgatttcggt aaaagaaaac gattttatta cagaagaaaa atttcaaaaa
       61 attatacaat ccaaagatac ggagacattg gcatttatct tagaatcaac tccctatcat
      121 ttatcgattg acatcttaga agatcctagt cagacagaga tttcgctaat gacaaaatta
      181 gtcaatgatt atagatgggc ctatgctgaa agtccgtctg atataattgt gactttattt
      241 gctttacgat atgtttatca taatatcaaa gttttattaa aatctaaggc ggcaattaag
      301 aaagattttt ctaaattatt aattccaata gggatttttg atatagaaag tttaaaacat
      361 ttagtttctt ccttacattc agatacactt cctgatttta tggttcgtga agtagaatca
      421 atttggaatg agtatgaaac ttttaataat attcgtgtac ttgatgtcgg agctgatcta
      481 gcatatttta aacatctgaa acttttatct aatgagttag atgaggtact gtctcaggtt
      541 attgtcgaaa tgattgactt ttataatatt attactgtaa aacgtggttt atctcaaaat
      601 aagagtcatg gggatatttt acaattactt tcagatgaag gaagtatttc tgctaaagaa
      661 tttatataca ttgtagaaaa tcaagaaata tttgtgtggt tcaataaaat aaatccaagc
      721 ttagattcaa tcttttcaac ttatgaattg aagatgcagg acgcaacaat ttcatcttct
      781 gagttagaat ttttatgtga tttactattg tataaaactt tagatcaagg aaggtacaat
      841 gtagaggggc cgttagttct tgctagatat ttattgggat gtgagtttga agtaaagaat
      901 ctcagaatga tcatatcagc tcttcaaaat acaattccct ttgaatcaat aaaagaaagg
      961 atacgcccac attatggaag ctaataagta taaaattggc ataattggta gccgtgatat
     1021 tattttacca tttagcatga ttgggtttga tatatttcct gcctaccaag aacaagaagc
     1081 tataaataca ctaagaaaat tagctcaatc tgattatggt gtcatttata tcactgaaga
     1141 cattgcttca atgatattag atacaattcg ccattatgat tcccaagttg tgcctgctat
     1201 tattttatta ccgactcata aacaaggttt aaatttagga ttaaaacgta tagaggataa
     1261 tgtagagaaa gcagtaggac acaatatttt ataataatgt acaaaattgt ctgtaatatt
     1321 attctataat ttttggactt agtaaggaga ataactttga ctcaagggaa gattataaaa
     1381 gtatcgggac ctctagttat tgcatcaggt atgcaggagg ctaatattca agatatttgc
     1441 cgtgtaggta agctagggtt aatcggtgaa attattgaaa tgagaagaga tcaggcatct
     1501 atccaagtct atgaagaaac atctggtctt ggtccgggag aacctgttgt tacaactgga
     1561 gaacctctct cggttgaatt agggcctgga ttgatttctc aaatgtttga tggcatacaa
     1621 cgcccattag atcgatttaa attggctact cataatgatt ttctagttcg tggggtagaa
     1681 gttccaagtt tggatagaga tattaagtgg cattttgatt ccactatatc aattggtcaa
     1741 aaagtgagta cgggtgatat tcttggaact gtcaaggaaa ccgaagtagt taatcataaa
     1801 attatggttc cttatggaat atctggcgaa gtcgtttcta ttgcctctgg cgattttaca
     1861 attgatgaag ttgtatatga aataaaaaaa ttggacggta gtttctataa aggaacactt
     1921 atgcaaaaat ggcctgtccg caaggcgcgt cctgtttcta aacgtttaat cccagaagaa
     1981 ccattaatca caggtcaacg agttattgat acattctttc cagtaaccaa agggggagca
     2041 gcagctgttc ctggaccgtt tggagcagga aagacagttg tacaacacca agtagctaaa
     2101 tttgccaatg ttgatattgt tatttatgtc ggttgtggag aacgtggaaa tgaaatgacg
     2161 gatgtactga atgagtttcc tgagttgatt gaccctaata caggacaatc aattatgcaa
     2221 cggacagttc tgattgctaa tacttcaaat atgcctgttg ctgctcgtga ggcttcaatt
     2281 tatacaggaa ttaccatggc tgagtatttt cgtgatatgg gctactctgt cgccattatg
     2341 gctgattcga cttcacgttg ggcagaagcg ctacgtgaaa tgtcaggacg tctagaagaa
     2401 atgcctggtg atgagggtta tcctgcttat cttggaagtc gtatcgctga atattatgaa
     2461 agagcgggac gttctcaggc tctagggctt ccagaacgtg aaggaacgat tactgctatt
     2521 ggagctgtat cgccacccgg tggagatatt tcagaaccag ttactcaaaa cactttacgg
     2581 attgtgaaag ttttttgggg gcttgatgct ccgttggcac agcgacgtca ttttcctgca
     2641 attaactggc ttacctctta ttcactctat aaagacagtg cgggaactta tatagatgat
     2701 aaagagaaga cagattggaa tagtaaaata actcgtgcga tgaactactt acaacgggaa
     2761 tctagtttag aggaaattgt tcgtcttgtt ggaattgatt ctctgtctga aaatgaacga
     2821 ctaacgatgg aaattgctaa acaaattaga gaagattatt tgcaacagaa cgcttttgat
     2881 tcggtagata cattcacttc gtttgcaaaa caagaagcaa tgttaagtaa tatcctaact
     2941 tttgctgatc aggcaaatca tgctttagag ttgggttctt actttacaga gattatagaa
     3001 ggtaccgtgg cagttcgaga ccgtatggcg agaagtaaat atgtttcaga agatagatta
     3061 gatgaaatca aaattatttc aaatgagatt acacatcaaa ttcaattgat attagaaaca
     3121 ggaggtctat aaatgagtgt tataaaagaa tatagaactg ctagtgaagt tgttgggcct
     3181 cttatgattg ttgaacaagt taataatgtg tcttacaatg agttagttga aattcaactt
     3241 cataatggag aaattcgtcg tggacaagtt ttagagatcc acgaagataa agcaatggtt
     3301 cagctttttg aaggatctag tggaataaat ttagaaaagt ctaaaattcg ttttgctggt
     3361 catgcactag aattggctgt atctgaggat atggttggta gaatttttaa tgggatggga
     3421 aaaccaattg atggtggacc agatttaatt ccagagaaat atttagatat tgatggtcaa
     3481 gctattaatc ctgtatctag agattatcca gatgaattta ttcagacagg gatctcctct
     3541 attgatcatt tgaatactct tgtacgtggt caaaaattac cagtattttc aggttcgggc
     3601 ttacctcata atgaattagc agctcaaata gcaagacaag cgactgtttt aaattctgat
     3661 gaaaattttg cggttgtatt tgcagcaatg ggtattactt ttgaagaagc tgagttcttt
     3721 atggaagaac tcagaaaaac aggagcgatc gatcgttcag tcttatttat gaacttggca
     3781 aatgatcctg caattgagcg tattgcaact ccccgcattg ctttaactgc ggcagagtat
     3841 ctagcttttg aaaaagatat gcatgttcta gttatcatga cggatatgac taactattgt
     3901 gaagcgttac gtgaagtctc ggcagctcgc cgtgaagttc caggtagacg aggctatccg
     3961 ggatatttat atacaaattt atcaactcta tacgaaaggg ctggtcgctt agttggtaaa
     4021 aaaggttcag tgacacagat tcctatttta acaatgccag aagatgacat aacacatcca
     4081 attcctgatt taactggata cattactgaa gggcaaatta ttttgtcgca tgagttgtat
     4141 aatcaaggtt atcgtccacc aatcaatgtt ttaccttctc tctctcgatt aaaagataag
     4201 ggatctggag aaggtaaaac tcgtggagat catgctccaa ctatgaatca actgtttgca
     4261 gcctatgccc aagggaaaaa ggttgaagag ttagcagtag tattaggaga atcggcttta
     4321 tctgatgtag ataaattgta tgtgaagttt acaaagcgtt ttgaagaaga gtacatcaac
     4381 caaggatttt ataaaaatcg aaatattgaa gatacgttga atcttgggtg ggaattacta
     4441 tcaattcttc ctagaacaga gttaaaacgt atcaaagatg atttgcttga taaatactta
     4501 cctttggtag aagtttaatc cagaaatgga gtgattatct atggtgcgtt tgaatgtaaa
     4561 accaactcgt atggaattga ataacttaaa ggaacgtttg aaaacagctg aacgtggaca
     4621 taagttatta aaggataaaa gagatgaatt gatgaggcga tttatttctt tgattcgtga
     4681 gaataatcaa cttcggaaag aagtagaaag ttatctaatt gataatctaa aagcctttgc
     4741 agttgctaaa tcattaaaga actctctaat ggtggaggaa ttattttcaa ttccatcgaa
     4801 agaaattgaa ttatttgttg agaaagaaaa tatcatgagt gtaacagttc ctagaatgca
     4861 tatgaatatt acttctcaaa atgagaacag tgaatacagc tatttatctt ctaatagtga
     4921 aatggatgat gtatttgcta caatgaatag tttaattgat aaattactaa gactggcaga
     4981 agttgaaaaa acgtgtcagt taatggctga tgaaatagaa aaaacacgta gacgtgtaaa
     5041 tggattagaa tattcgatta ttccaaactt gtcagaaact attcattata tagaattgaa
     5101 actagaggag gcagaaagag ccaatttagt tcggattatg aaagtgaagt agatccttta
     5161 tttagatttt aattagatga accaatatca gcttggataa ggcggtaagc ctttctaagc
     5221 ttttttattg acagtatcag gatatctttt tcaaaatttt ggtttgttag ataatgaaaa
     5281 tgtttctacc gatttagatt taggattaat aagtcgtaaa tgtaatttta aagaaaaaga
     5341 taagttaaaa aaagatgtat tagcaagagt tggtctaact catattcatc tagaacaaaa
     5401 gatatatgaa ttgtctggcg gtgaagcata acgtgttgct ttagcgaaaa tcattcttaa
     5461 aaatccacca ttaatactcg cggatgagct gactgcatca ctcgatccga cgacttcaca
     5521 aggaattatg aaattattat tagatatgaa aaatgaaaat cgtctaatca tcattgcaac
     5581 tcataatccc gatatttgga atcaagcaga tgaagttata aa
//
LOCUS       DAVKJJ010000082         5600 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000082 DAVKJJ010000000
VERSION     DAVKJJ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.082"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            122..781
                     /locus_tag="VW144_001929"
     CDS             122..781
                     /locus_tag="VW144_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEU9601330.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            806..1873
                     /locus_tag="VW144_001930"
     CDS             806..1873
                     /locus_tag="VW144_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9601331.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            1977..2987
                     /locus_tag="VW144_001931"
     CDS             1977..2987
                     /locus_tag="VW144_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9601332.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            2999..4690
                     /locus_tag="VW144_001932"
     CDS             2999..4690
                     /locus_tag="VW144_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9601333.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            4693..5403
                     /locus_tag="VW144_001933"
     CDS             4693..5403
                     /locus_tag="VW144_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU9601334.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         1846 a          771 c         1068 g         1915 t
ORIGIN      
        1 gcatatttaa tgaaagataa atttaaaaaa atgtttgcaa gtgcttgaca aaggaaacgg
       61 tttcattgta taatgtaaac atgatgacac cgatgtcaaa tataaaaaag aaggtcttta
      121 tatgaaaaac ttaacaaaaa ttaagttcaa agagaatggg gaatttaatc atttccctgg
      181 gaatacagtt gtagcaaatc tttatactaa acaagatttg atggaagttg ttgatattat
      241 tcaatcacgt tatagagaat taccatttat cgataagttt actttaactc caaggaattc
      301 tattcacatg actgtaattg aattgttgtg ccatgaaaat cgtgaaacgg agttttggag
      361 cagtaatctt cctctagata cacctttaca ggaaatacat gattactttg ctaaacaact
      421 tgaaattttt ccattgttgg atgaagaaat tcatatgcgt ataactgaaa tgggaaaaca
      481 aaacatacta gttgaacctg cagatgaagc ttctgcaaag agattagaag aaattcgtac
      541 ttatgtctca gaaaaagcag gtgtttgttt ccctaatcat gatagatatc aattccatat
      601 ttcaattggg tatcttcgga ttcctctaac cgaagaggag gaagaagagt ttactaaagt
      661 cagagcagaa ttaactgaaa ttttattaga gaagattcca actattactg taaaccgtat
      721 tgactatact gtatttgaag atatgagaca atttgttcca tatcacgaaa aatttaaata
      781 aaataataaa aaaggagaac ttatcatgaa aaaaatgaaa gtttggtcta ctgtacttgc
      841 aacgggagtt gctcttacta cacttgctgc ttgctctgga ggttcaaatt ctacgactgc
      901 ttcttcatct gaagaaaaag ctgataaaag tcaagaatta gttatctatt cgaactcagt
      961 ctcaaatggt cgtggtgatt ggttaactgc taaagcaaaa gaagctggtt ttaatataaa
     1021 aatggttgat atcgctggcg ctcaattagc agaccgtgtt attgctgaga agaataatgc
     1081 agttgcagat atggtatttg gaattggtgc tgttgactca aataaaatta gagatcaaaa
     1141 attactagta cagtacaagc ctaaatggtt agataaaatt gatcaatctt tatcagataa
     1201 agataattat tataatcctg tgattgttca accattagtt ttaattgggg cgcctgatgt
     1261 aaaagaaatg cctaaagatt ggactgaatt aggtagtaag tataaaggta aatattcaat
     1321 ttatggtctt caaggtggta caggacgggc aattctagca agtatcttag ttcgttacct
     1381 tgatgaaaaa ggagatttag gcgtatcaga aaaaggatgg gaagttgcaa aagaatactt
     1441 tgcaaatgcc tatactcttc aaaaaggtga aagctcagtt gttaaagcgt tagataaaga
     1501 aagcccaatt caatacggta tgatctgggg ttctggagcg ttaattggac aaaaagaaca
     1561 aaatgttgaa ttcaaagtta tgactcctga gattggtgta ccatttgtaa ctgaacaaac
     1621 tatggtttta agcactagta aaaaacaagc gttagctaaa gaatttattg attggtttgg
     1681 tcaatcagaa attcaagtag aatatagtaa aaaatttgga tctattcctg caaataaaga
     1741 tgccctaaaa gagttacctg aagatacgaa aaaatttgtt gatcaagtga aaccacaaaa
     1801 tattgactgg gaagctgttg gaaagcattt ggatgaatgg gtagaaaaag ctgaattaga
     1861 atacgtaaaa taagaaataa gagttatttt ttatgtccaa gtctctctat tatttctaaa
     1921 tttaaggaag aaataaagag aggcttttgg ttttagtaat agagggagtt agtgagatga
     1981 ttaaatttga taatattcaa attaaatatg gtgattttgt tgcaattgat aatctgaatt
     2041 tagatataca tgaaggggaa ttctttacat ttcttgggcc ttcaggatgt ggtaaatcaa
     2101 ctactttgag agcattggta ggttttttag atccatcatc aggaagtatt gaagttaatg
     2161 gaacagatgt cactcatttg gaacctgaaa agcgtggaat tggtattgta tttcaatctt
     2221 atgcgctatt tccaactatg actgtttttg ataatattgc atttggttta aaagttaaga
     2281 aggtagctcc agatgttatt aaagctaaag tatcagcagt ggcagcaaaa attaagatct
     2341 ctgatcaaca gttacagcgt aatgtatcag aattatctgg gggtcaacaa caaagggtag
     2401 cattggctcg tgctctggtt cttgaaccta aaattctttg tctagatgaa ccattgtcaa
     2461 accttgacgc aaaattacgt gtagatttga gaaaagagtt gaaaagactt caaaaagagt
     2521 taggtattac tactttatat gttactcatg atcaagagga agccttgact ttatctgata
     2581 gaattgcagt ctttaacaat ggatacatcg aacaggtcgg tacaccagta gagatttatc
     2641 ataattctca aactgaattt gtatgtgatt ttattggaga tattaatgtt ttgaccgatg
     2701 aaacagtcca cgaagtatta ttgaaaaata caagcgtttt cttagaggat aaaaaaggat
     2761 acattcgatt agagaaagtt cgattcaatc gtgaaactga acaagatttt attctaaaag
     2821 ggacaattat tgatgttgag ttttctggag ttacaattca ctatacaata aaagtttctg
     2881 aaagtcagat tcttaatgta acaagtattg atagtcaggc tgctattaga tctgtcggag
     2941 aaagtgtgga attatttatc acaccatcag acgttctgca attttaagga ggtgcagtat
     3001 gcgtcataaa ttaaatttaa aagattggct tattcgttta gggttaatct ggttcttagt
     3061 aacatttatt atttatccaa actttgatct agtagtgaat gtatttgtaa aaggaggaga
     3121 attttccctt gatgctgtac atcgtgttct aaaatctcag agggcacttc agagtattat
     3181 gaacagtttt aagttagcat tttcactcat tattacagtt aatgtcgtag gtattctttg
     3241 tgttctattt acagagtact ttgatattaa aggtgctaaa attttaaaat taggttatat
     3301 gacctcttta atttatggag gagtggtttt agcgactgga tataaatttg tctatggtcc
     3361 ttatggattg attacaaaat ttttacaaaa tgttatccct tctttagact ctaactggtt
     3421 tattgggtat ggtgcagtct tattcattat gacattttca ggaactgcta atcatacatt
     3481 gtttttaaca aatacaattc gaagcgttga ctatcacact attgaggctg ctcgaaatat
     3541 gggagcaaaa ccatttactg ttttccgaaa agtagtgtta ccaaccttaa ttccaactct
     3601 atttgcactt actattatgg tttttcttag tggtttatct gcagtagcag cacccatgat
     3661 tgttggtggt aaagaatttc aaactataaa tccaatgatt attacatttg cagggatggg
     3721 gaattctcgt gatttagctg ccttacttgc aattatttta ggtattgcaa ctacaatttt
     3781 gcttactatc atgaataaga tagaaaaagg tggaaattat atttctatct ctaagactaa
     3841 agcgcctctt aaaaaacaaa aaattgcgtc taagccttgg aatatcattg ctcacattgt
     3901 agcatatgga ttgttcacag ttttcatgct tccactaatt tttatagtat tatactcatt
     3961 tacagatcca gttgcaattc aaacaggtaa cttaacatta tcaaacttta ctttagaaaa
     4021 ttatcgctta ttctttagta atagtgcggc attctctcca ttcttggtca gctttattta
     4081 ttctattatt gctgcgacaa cagcaacaat tctcgcagtt gtatttgctc gtgttgtcag
     4141 aaaacataaa tctcgttttg atttcttatt tgaatatggt gctctacttc cttggttatt
     4201 accaagtacg cttttagcag taagtttatt atttactttt aatcagccac aatttcttgt
     4261 cttgaatcag attttggtag gtagtttggt aattctactt attgcatata tagttgtaaa
     4321 aatcccattt tcttatagaa tggtacgtgc tattttattt agtgttgatg atgagatgga
     4381 agatgcagca agaagtatgg gtgcttcacc tttttatact atgatgaagg ttatcattcc
     4441 atttatttta ccggttgttc tctctgttat tgctttaaac tttaactctt tattaactga
     4501 cttcgactta tctgtattcc tttaccatcc cctagctcaa ccattaggta ttacgattcg
     4561 atctgcaggt gatgaaacag caacatctaa tgcacaagct ctggtatttg tttatacaat
     4621 tgttctgatg attatttctg gaacggtatt atacttcaca caaagaccgg ggcgtaaagt
     4681 aaggaaataa tcatgacagc cactagtctt gggttatcaa atattgaaat agttgtcagg
     4741 attgttttat cagtagtcat tggtagtata attggtttag agagagggag caaatcccag
     4801 cctgcaggca tccgaactta tagtattgtt tgtctagctg catgtttgat tatgatgacg
     4861 aatgaatacg tatcttataa atttgggaca ggagatccta cacgattagg agctcaagtt
     4921 atatcaggtg tgggttttct aggcgctgga acgattctta ttacagataa aaagaaaatt
     4981 acaggtctga caactgcagc aggcatttgg gcttcggcag gaattggatt agctattgga
     5041 gtaggttttt atgagggagc tcttttagta gccatttctg tttggggtgt gatatccatg
     5101 ttccaaccac taaaaaaata tctacaaaat cgttctaaaa tgattgaatt gtatatagtg
     5161 gttaaatcta cagaagccta taatcgtgta ttggtttatt gtgcagaaaa tggtatcaga
     5221 atgaccgatt caagaacagc gtttggagat gttaattctg acagaattga atactttgat
     5281 gttccagata aaaaaatagc gtcatttatt actctggaat tatcaggtag atttgaacat
     5341 cttcgactga tggaagaaat tgctaatatt gtaggtgtta tttatgtcga agaaattagc
     5401 tgaaatttag taaacaaagg gacagatgtt tttgtccttt tgttttttag atgttttaat
     5461 catctaattt ttgagtagat ttttgctcag aggtttttat gaaagattat aaaatcaatt
     5521 ttgatttagg taaaatagaa tattttgata ataattgttt gatacaagta tacaaattta
     5581 tttcttttta tgatatttgt
//
LOCUS       DAVKJJ010000083         4818 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000083 DAVKJJ010000000
VERSION     DAVKJJ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.083"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            84..881
                     /locus_tag="VW144_001934"
     CDS             84..881
                     /locus_tag="VW144_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEU9601335.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSLLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1029..1151
                     /locus_tag="VW144_001935"
     CDS             1029..1151
                     /locus_tag="VW144_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEU9601336.1"
                     /translation="MTRDFKFETLQLHAGQVVTPATKSRAVPIYQTTSFVFDDT"
     gene            1173..2288
                     /locus_tag="VW144_001936"
     CDS             1173..2288
                     /locus_tag="VW144_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HEU9601337.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAH
                     AGEHVVAASTIYGGTFNLLKEPLPRYGITTTFVDVDNLEEVEAAIKDNTKLVLIETLV
                     NPLINIPDLEKLAEIAHKHQIPLVSDNTFATPYLINVFSHGVDIAIHSATKFIGGHGT
                     TIGGIIVDSGRFDWTASGKFPQFVDESPSCHNLSYTHDVGAAAFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETLSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYYALA
                     EKYLPKGVGSIFTFHVKGDEEEARKVIDNLEIFSDLANAADAKSLVVHPATITHGQLS
                     EKDLEAAGVTPNQIHLSIGLENVEDLIEDLRLALEKI"
     gene            2384..3658
                     /locus_tag="VW144_001937"
     CDS             2384..3658
                     /locus_tag="VW144_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEU9601338.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEV
                     QALENQLATLRLEHENQLQKTLSDLEKERNQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3670..3867
                     /locus_tag="VW144_001938"
     CDS             3670..3867
                     /locus_tag="VW144_001938"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601339.1"
                     /translation="MEDDLNYENLMDDVTEAIKKFNLVIFIGAGVSIAQGYPNWNNYI
                     EHLIKYWQGQVLSVSNVKWIL"
     gene            3855..>4818
                     /locus_tag="VW144_001939"
     CDS             3855..>4818
                     /locus_tag="VW144_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEU9601340.1"
                     /translation="MDLVNYELKKVFGEDFEKRRLDFEKGYFKNLLPYSIVNQTVESL
                     ASLNAIFITSNYDYEIENHIKRLKNAVVTINDLNEFTKNKNGKLQFGDVLHIHGTPDC
                     DVKYFVSSSADYSKTYLKNRENFENLVTWFKETKPTVLFVGAGLEEDEILSLLCKDSK
                     NYALMKSENTGNQRVDEHYRGVVEGFFSSENHTQIIWYGDEFEKLPLFVKKLVADINE
                     KLGTHDFYNQWNNLLNPSINQEEYNKNLDSISNDFKYLSSVLDKVIENDNNQLDQLML
                     NALLRSETLTVIKKNFVLAFWRFIVKNIEKLSDNEWDVIYKIIYE"
BASE COUNT         1552 a          798 c         1008 g         1460 t
ORIGIN      
        1 acttttatga aatatagaaa acgcttccta aaacctaaag tttgtgatat aattatcaag
       61 aaaaaaagat ttaggagttt attatggttt cttcagaatt tatctcaaag attgaatttg
      121 cttgcaataa gaaagaaagt ctttatagtc aaagcaaatt taagtatgcg attcgttcga
      181 tgttcgcagg tgcattttta accttcagta ctgctgcagg tgcagttggg gctgacttga
      241 ttaataaaat tgcaccaggt agtggacgct tcctctttcc attcgttttt gcttggggct
      301 tggcctacat tgtttttttg aatgccgagt tggtcacttc aaacatgatg ttcttgactg
      361 ctggtagttt attaaaaaaa atctcttgga gaaaaacagc tgagatttta ctatactgta
      421 ccttgttcaa ccttatcgga gccttgatag cagggtgggg ctttgctcat tcggcagcct
      481 atgcgaatct gacacacgat agtttcatct caggggttgt agagatgaag ttaggccgct
      541 caaatgaatt ggtcttgctt gaggcgattt tggcaaatat tttcgtaaac attgccattc
      601 tttcatttat tttggtcaaa gatggtggtg ccaaactttg gcttgtgttg tcagctattt
      661 acatgtttgt attcttaaca aacgagcaca ttgcggcgaa ctttgcttct ttcgcgattg
      721 tgaaattcag tgttgctgcg gattcaattg ccaacttcgg tgttggaaat atgcttcgcc
      781 actggggtgt gactttcatc ggaaacttta tcggaggagg cctcttgatg ggtcttccat
      841 atgccttcct caataaaaac gaagatactt atgtagatta agaaaatgag cacgattgag
      901 tcgtgctttt ttcattttca aaataagata atagctattt cttatatcaa aatatagaaa
      961 actgatattt gtaaactata actcaaggtg ctacaatatc cttaataaaa taatatggag
     1021 gtcaccttat gactcgtgat tttaaatttg aaactctaca actacatgct ggtcaagttg
     1081 tgactccagc tactaagtct cgtgcagtgc cgatttatca aacaacatct tttgtttttg
     1141 atgacacgta ggaaggtgct gatctgtttg ccttgaggaa accagggaac atttatactc
     1201 gtatcaccaa tcctacaaca gctgcccttg aaggtggtgt tgaagcgcta gcaacagcat
     1261 caggtatgac tgcagtgact tatacgattt tggcgcttgc ccatgctggt gagcatgtag
     1321 tggctgcttc gactatttac ggtggaacct tcaatctttt gaaagaaccc cttcctcgtt
     1381 atggtatcac aacaaccttt gtcgatgttg ataatttgga ggaagtagaa gcagctatca
     1441 aagacaatac caagcttgtc ttgattgaaa ccttggttaa ccccttgatt aatattccag
     1501 acctggaaaa actggcagag attgctcata aacatcaaat cccacttgtg tcagacaata
     1561 cttttgcaac accttatttg attaacgtct tctctcatgg cgttgacatt gccattcact
     1621 ctgcgactaa gtttatcggt gggcatggta caactattgg aggaataatt gtcgatagtg
     1681 gtcgttttga ctggacggct tcagggaaat tccctcaatt tgttgacgag agtccaagct
     1741 gccacaattt gagctatact catgatgtgg gtgcagcagc ctttattata gctgttcgag
     1801 ttcaattgct tcgtgataca ggtgcagcct tgtcaccatt caatgctttc ctcttgctac
     1861 aaagacttga aaccctttca cttcgtgtgg aacgccatgt acaaaatgct gagacaattg
     1921 ttgattttct tgtcaaccat cctaaggtag aaaaggtaaa ttatccaaaa cttgcagata
     1981 gtccttatta tgccttggct gagaaatact tgccaaaagg tgtcggttca atctttacct
     2041 tccacgtcaa aggtgacgag gaggaagcac gcaaggtcat tgataattta gaaatctttt
     2101 ctgaccttgc aaacgcggca gatgctaaat cgcttgttgt ccatccagca acaatcactc
     2161 acggtcaatt gtcagaaaaa gacctagaag cagcaggtgt cacaccaaat caaatccact
     2221 tgtctatcgg tcttgaaaat gtagaagatt tgattgaaga cttacgcttg gccttggaaa
     2281 aaatttaaag taaaagaaga taaacagtgg gttttgactc actgtttttg attttccctc
     2341 aggcatgata taatggttac agaagtctag aaagaggaat gtaatgaacg aaatcaaatg
     2401 tcccaactgt ggggaagtct ttacagtaaa tgaaagccag tatgctgaac ttttgtccca
     2461 agtgagaacg gcagagtttg ataaggaact acacgatagg atgaggcagg aactggcctt
     2521 ggctgagcaa aaggctatga atgagcaaca gactaaactg gctcagaagg atcaagaaat
     2581 tgcgcaattg cagagtcaaa tccaaaactt tgatacagaa aaagaattgg ccaagaaaga
     2641 agttgaacag acaagccatc aggccttatt ggctaaggac aaggaagtac aggccttgga
     2701 aaaccagttg gcgaccttgc gtttggagca tgaaaatcaa ttgcaaaaga ccctttctga
     2761 cctagaaaaa gaacgcaatc aggtcaaaaa tcagctccta ctgcaagaaa aggaaaatga
     2821 gttgtctttg gcttctgtta agcaaaacta cgaagcccag ctcaaagcag ccagtgaaca
     2881 agtcgagttt tataagaatt ttaaggctca acaatctaca aaagcgattg gggaaagcct
     2941 agaacagtat gcagagagtg agtttaacaa ggttcgtagt ttcgcctttc caaatgctta
     3001 ctttgagaag gataacaagg tctcttcgcg tgggtctaaa ggggacttta tcttccgtga
     3061 gtgtgatgaa aatggagttg aaatcatttc tatcatgttt gagatgaaaa acgaagcgga
     3121 cggaacagag aagaagcaca agaatgcaga tttttacaag gaattggaca aggaccgtcg
     3181 ggagaagaac tgtgagtatg ccgttttggt gaccatgctt gaggctgata atgactactt
     3241 taacacaggg attgttgacg tcagtcacga gtatgaaaaa atgtatgttg ttcgtcctca
     3301 attctttatc caattgattg gtctcttacg taatgcggcg ctaaattccc taaaatacaa
     3361 gcaggagttg gccttggttc gcgagcaaaa tattgacatt acgcattttg aggaagactt
     3421 ggatgccttt aagctagctt ttgctaagaa ctataattca gcttcgacta actttggaaa
     3481 agctattgat gaaatcgaca aggccatcaa acgcatggaa gaggttaaga aattcctgac
     3541 tacatctgaa aaccaactcc gtctagctaa caacaaattg gaagatgtct ctgttaaaaa
     3601 attgacccgg aaaaatccaa caatgaaagc gaagttcgaa gcattgaagg gggagtgagg
     3661 aaataagtta tggaagatga tcttaattat gaaaatttga tggatgatgt aaccgaggct
     3721 ataaaaaagt ttaacttagt aatttttatt ggcgcaggag tttcaattgc tcaaggttat
     3781 cctaattgga acaattatat tgaacatctt attaagtatt ggcaaggaca agttttatcg
     3841 gtaagtaacg taaagtggat cttgtaaact atgaattaaa aaaagttttt ggtgaagatt
     3901 ttgaaaagcg acgactagat tttgaaaaag gatattttaa aaatctatta ccatattcaa
     3961 tagttaatca aacagtagaa tctttagcaa gtttgaatgc aatatttatt acttctaact
     4021 acgactatga aattgaaaat catattaaac gtttaaagaa tgctgtagtt actattaatg
     4081 atctcaatga attcactaag aacaagaatg ggaaactaca atttggtgat gttctacata
     4141 tccatggaac accagattgt gatgtaaagt attttgtaag ttcatccgca gattattcaa
     4201 aaacctattt aaaaaatagg gaaaattttg aaaatttagt aacatggttt aaagagacaa
     4261 aacctactgt tttatttgtt ggagcaggtc ttgaagaaga tgaaatttta tctttacttt
     4321 gtaaagatag taagaactat gccttgatga agtcagagaa cacaggtaat cagagagttg
     4381 atgaacatta tagaggtgtt gttgaaggat tttttagctc agaaaatcat actcagatta
     4441 tttggtacgg agatgaattt gagaaactac ctctttttgt aaaaaaatta gttgctgata
     4501 ttaatgaaaa attgggcaca catgattttt ataatcagtg gaacaatctt ttgaatcctt
     4561 ctataaatca agaagagtat aataaaaatc tagatagtat ttctaatgat tttaagtatc
     4621 taagctctgt tttagataag gttattgaaa atgataataa tcaactagat cagctaatgt
     4681 taaatgctct gcttcggagt gaaactctaa cagtaattaa aaaaaatttt gtccttgcat
     4741 tttggaggtt tattgttaag aatattgaaa agttaagtga taatgagtgg gatgtgattt
     4801 ataaaataat ttatgaag
//
LOCUS       DAVKJJ010000084         4748 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000084 DAVKJJ010000000
VERSION     DAVKJJ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.084"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            537..1400
                     /locus_tag="VW144_001940"
     CDS             537..1400
                     /locus_tag="VW144_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9601341.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVTCAGLTTSQLSKFELGQSM
                     LSADKLILAIQGINMNFDEFGHKLNNYQESPHMRFGRKVVDRFAHQDIAGLEQLLEEV
                     EQEQMAETYRRLNAIVIKDAIHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNIISELMERKLFSYIPIFE
                     AELESMLRPYDVFEKLLWQFLKKMSIFLQTKGSNQKEIEHFIQSLQVLENPQLIALFE
                     LRLQQYKKLID"
     gene            1686..1919
                     /locus_tag="VW144_001941"
     CDS             1686..1919
                     /locus_tag="VW144_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601342.1"
                     /translation="MLNLQFAETMELTEAELQDVRGGNLVNSMGGGGRSGISGWGVPG
                     IYPGWGNQGMSPNRGAFDWTIDLADGLFGRRRR"
     gene            1941..2618
                     /locus_tag="VW144_001942"
     CDS             1941..2618
                     /locus_tag="VW144_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9601343.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMVALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWFYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATNLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVKRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            2630..3295
                     /locus_tag="VW144_001943"
     CDS             2630..3295
                     /locus_tag="VW144_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9601344.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEEIIHRGLVMTVLEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            3367..4593
                     /locus_tag="VW144_001944"
     CDS             3367..4593
                     /locus_tag="VW144_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9601345.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQILQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPISTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
BASE COUNT         1352 a          818 c          922 g         1656 t
ORIGIN      
        1 tagtatagat aacgtaattt ttgaaaagaa ttttgaacta tttacaaaac aaagtgatct
       61 aattgttgca aatagatggg ataggggcct tgaagcttat aaagataaag tttacacaag
      121 ggggatctgg ataagagatt aactattgat attaaaaagg caataattta taaaatttgt
      181 acaaaaaaat gttaaatttt cacattttca tagtatactg tttaatagaa agtaggttct
      241 ttgtaaccgc tccattataa aatcctttca ctcttccggt atactattag gaaaaacata
      301 actggaggat attttgagct cacacctcat cgttccagtg gctgttcccc aagctcgtgt
      361 actgcccaaa aaagttagac attctattta gaacgaggat tgagttctgt attgtacaag
      421 gctcggtcct tttagagtca gcttaaggct ggctttttca atcaccaaag tgtcagaatg
      481 ttttgacaaa tgaacacaaa taatgatata atatgcaaaa gctaggaggt ggtaggatga
      541 ttgaaaaaat ggaactggga gaattttaca aagaactgcg cctggctaga aagctcaagc
      601 agtcagatgt gacttgtgct ggtctaacaa cctctcagtt gtccaagttt gaactagggc
      661 agtctatgtt gtctgcagat aagctcatat tagccatcca agggatcaat atgaattttg
      721 atgagtttgg gcacaagctc aacaactatc aagaatctcc acatatgcga tttggtagaa
      781 aggttgtgga tcgctttgct catcaagata tagctggctt agagcaactg ttggaggaag
      841 tcgagcaaga acagatggca gagacttatc gtcgtttgaa tgctattgta atcaaagatg
      901 ccatccactc actagataaa agctacccac tagcagagga ggatagcgag tttttgacca
      961 cctatctcta tgctatcgag tcttggacct ggtttgaact ctatctcttt tgcaatacta
     1021 tgcccttctt gagcaatcaa gatctgatct ttttatcaac ctccttactc gaaaaatcca
     1081 aagaatttaa agagttagta cacaatcgat tgtatatgaa gcaaggactc ttaaatatca
     1141 tctcagagct catggagcgc aaactttttt cttatatccc aatctttgaa gccgagctgg
     1201 agagtatgct ccgtccatac gatgtttttg agaaattatt gtggcagttt ttaaagaaga
     1261 tgagcatttt ccttcaaact aagggaagca atcaaaaaga gattgaacac tttatccaat
     1321 ctctgcaagt attagaaaat ccacaattaa tagccctttt tgaattgcgt ttgcagcaat
     1381 acaaaaagct tatcgattag ttagaaatgg caaaataata ataatctcag caacgatttt
     1441 caataatgtt aaaatttgtt tcttcatatt tctccactcc tcattagtag taactttatt
     1501 ataccagaga agtaatcaaa ttcaccaaaa tcgcaaatct gaaatttcta taagaaaaaa
     1561 tatcaaatat gcgattttct aaaataagcc aattttcgtg ttatactgta cttgtaaagc
     1621 acttgaagca aatcctaggt cgcagaagtg gtttacaaat gaagataatt gaaggagtgt
     1681 aaaagatgtt aaatttacaa tttgcagaaa caatggaatt gacagaagct gagttgcaag
     1741 atgttagagg aggcaacctc gtaaattcta tgggaggtgg tggaagaagt ggtatatctg
     1801 gttggggcgt cccaggaatt taccctggtt ggggcaatca agggatgagt cctaatcgtg
     1861 gagcttttga ttggactata gatttagcag atggattgtt tggccgccgt agaagatagg
     1921 ctcttatagg agataatagg atgaagaaaa tgaaagaagt gaaatttcac ctagcaactg
     1981 gcttgttgat tttgacttat tacctcattt ttaatgtcac atcagactta gattttatgg
     2041 ttgctttgtc tgataatatg tactatgtgt tccaagtttt attggtactt attctgggaa
     2101 ctatagctac aattgctttt gtcaaaagtg aacattggaa agagtgtggt cgtttccagt
     2161 ttcgttggtt ttacctgggt gttttccttc tttctttttt cttattgttc gtgtgggcaa
     2221 atctaacaac ctatatcttt cctcgtaccc agaatggaag tacagtcgta gaagtagcca
     2281 ccaatttaac aggaattagc tattttgtta cgcgaatact ttatactagt attatcgcac
     2341 ctgtatcaga agaggttgtt tgtcgtggtc ttttgatgac aagtttatct aaagttaaga
     2401 gatactatct tgatgttctt gtttccgctg ccatatttgg cgctatgcat gttttgcagt
     2461 atggttggat aacgacagat ttcataaagt attttggaat gggcttgata ttttgtatga
     2521 tgtttcgcta cacccgatcc atctattggg ctatagctct gcatgcttca tggaatagtt
     2581 tcttactcat tgttacttta ttggtatttg gatattagaa aggtgatata tgaaaaagat
     2641 aatctcacac cgctacttta ttatagtttt tctactagtt attgctgacc agaagttcag
     2701 tgttctagtt ttacgcagcg accttgttac tgggctatct gactttgcct attatctgtc
     2761 ggatatgatg ttgaattttc ttgtggtttt atttgctctt attgctatga tttggtcggg
     2821 gaaatggcag aaaatcaata gtagaaagtt taaaggatcc tatcttttct attccttcct
     2881 agctctgctt gcttttgttg tttggaattt cgttaccttt tttcttttcc cacctactcg
     2941 aaatgaaatt tcttatcaac atgctgctcc gacttttaca ggggctacag catttttgat
     3001 gtattttttc tatcctgtga ttgcaggtcc catttttgaa gagataatcc atcggggatt
     3061 ggtgatgaca gttctggaaa aaggaaagaa atgggggcta gatgtacttg gttccgctgt
     3121 tttatttggg gtctcgcaca ttagtaatca cggttgggtc ttgacagact ttgtcttcta
     3181 tatgggtgga ggtcttatat ttgcagtctt atttagaatg acaaagtcaa tttattggcc
     3241 tattggactg catatagtct acaatggcat tggtcagctt ttgatgttac tgtaattttg
     3301 gttgttagta gtatcgcggt ctatccttgg ggaaagcttc ccatttgaac ttaagaagga
     3361 gttgtgatga aagtatttct tcaaaataga gattttaggc aattaactat caaccagtgg
     3421 atttcaacgc ttggggatac gattttttat ctggcctttc tgaattatgt ggcagatgcg
     3481 tcttttgcac ctttggcgat tttactcatt acgatttcag aaacccttcc ccaaatttta
     3541 caaatctttc tgggagtttt ggcagatttt caacatcatc gtgtcttgaa atacacagtc
     3601 attagttttg caaaattttt gctttactct atcgtttctt tatcactttc agggcagtct
     3661 ttctccttgt tgctagtagc atttatttgt ctgcttaacc tcttgtctga cacattgagt
     3721 tacttttcag gagccatgct cactccgatt ttcattagaa ttattgggca agaccatctg
     3781 gcagaagcta ttggctttaa acagtcaact gttagtttag tgaaaacaat cagtaatatc
     3841 ctaggaggag tcttactagg cattctatcc atccagttta tttccttact gaacgctctt
     3901 acctttttaa tcgcattttt aggtatcctc ttcataaaaa ctgacctctt gaaagtagaa
     3961 aaaacgatta gctatcaaga aggactctct gtaaaatcct tttgccagca tttgctccaa
     4021 tcatcaaaat tgatatggaa tatgaataag gtgctcttgg ttttgtttat tatctctact
     4081 agtcaagcag tgataaatgt tacggttcct atttccactc tatttttaag gaaccagccc
     4141 tttttgaatt tacaaacagg tcaatctctt gccttgctat ccacttttga actgtcagcc
     4201 ctgattgtcg gaagccttgt aagtggctat ctgcaacata ccatttccat aaaaacagct
     4261 ctctatgcct cacttgtcat ccagttgctt cttctagtag gatttgccac agttcgtttt
     4321 gactggattc tcatctttag taccttggat gcctttttcg caggtgttct ctctcctaga
     4381 ttgcaagaac tcgtttttaa acaaatacct gaggagtcaa tgggagcagt tcaatcctct
     4441 atcggtgcca ttacggttgt tttacctagc ttatttacaa tagctttggt aaccattgct
     4501 actagctttg gaactctggc agttagcttt gttttattgc tatttcttct agttgccttt
     4561 gttatgcttc tgaatattcg tgaaagtatt tagtggagaa tttctataat atatgtttga
     4621 gactggattg ccagtctttt tatgatggtc aaaatcaaca gacttttttc tcttttctga
     4681 atgtgttata atagactgaa tctaaaatag tacgaaacaa ttgctaaaac atttatagaa
     4741 attaattt
//
LOCUS       DAVKJJ010000085         4690 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000085 DAVKJJ010000000
VERSION     DAVKJJ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.085"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(200..271)
                     /locus_tag="VW144_001945"
     tRNA            complement(200..271)
                     /locus_tag="VW144_001945"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(236..238),aa:Arg,seq:cct)
     gene            complement(314..661)
                     /gene="rplS"
                     /locus_tag="VW144_001946"
     CDS             complement(314..661)
                     /gene="rplS"
                     /locus_tag="VW144_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU9601346.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(780..1109)
                     /gene="crcB"
                     /locus_tag="VW144_001947"
     CDS             complement(780..1109)
                     /gene="crcB"
                     /locus_tag="VW144_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9601347.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(1103..1477)
                     /gene="crcB"
                     /locus_tag="VW144_001948"
     CDS             complement(1103..1477)
                     /gene="crcB"
                     /locus_tag="VW144_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9601348.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            complement(1474..1740)
                     /locus_tag="VW144_001949"
     CDS             complement(1474..1740)
                     /locus_tag="VW144_001949"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEU9601349.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(1856..2299)
                     /locus_tag="VW144_001950"
     CDS             complement(1856..2299)
                     /locus_tag="VW144_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEU9601350.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            2403..3338
                     /locus_tag="VW144_001951"
     CDS             2403..3338
                     /locus_tag="VW144_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEU9601351.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQKILKQYNITDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKVHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            3392..3676
                     /locus_tag="VW144_001952"
     CDS             3392..3676
                     /locus_tag="VW144_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEU9601352.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(3733..3951)
                     /locus_tag="VW144_001953"
     CDS             complement(3733..3951)
                     /locus_tag="VW144_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601353.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     IAHYGRVETAISFLPSSMEIEPTDESGT"
     gene            complement(3948..4690)
                     /locus_tag="VW144_001954"
                     /pseudo
     CDS             complement(3948..4690)
                     /locus_tag="VW144_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1454 a         1010 c          832 g         1394 t
ORIGIN      
        1 ttaaataatt tagaacttga cgattttgaa taaattgact atacaaacat ttatatactc
       61 gatatagtct caattttatc tgatgattgc ccaaattttt caataataaa acgcataata
      121 ttatggagac aatcccctat attatgcgtt cttttaatat caaagacttt ttgacaaact
      181 tctttgatat ctaattacat gccccctgca ggaatcgaac ctgcaactac tccttaggag
      241 ggagttgtta tatccattga actaagggag ctagataaaa actctgctaa atgagcagag
      301 ttttttagtc gaattaacga cggatttctt tgatacgagc tgctttacct tgaagagcac
      361 gcaagtagta caatttcgca cgacgtactt taccgtaacg aacaacttcg attttttcaa
      421 cacgtggagt gtggattggg aagatacgct caacacctac accgttagag attttacgaa
      481 ctgtgtagtt ttctgagatt ccagcacctt tacgtgcgat aacaacacct tcaaaaatct
      541 ggatacgttc acggttacct tcgacaactt tcgcatgtac acgaacagtg tcaccaggac
      601 ggaatgatgg gatatctgta cgaagttgac cttcagtcaa gctttggatt aatggattca
      661 ttttattctc ctatctttgt caatcttgag gaatcttcct cagcggataa actgtatttt
      721 tgtgcgtcca ttacacacga gatatagttt accaaatttc taaaaaaaag taaagaaatt
      781 tatagcaaaa ttcctaaaaa aatcgcaacc aaaccaccta tgtaagtcaa agtcaggtaa
      841 gaataaaaga ccttcttatc acttagcagt ctttgaagtt cgtcattcaa ggtcgaaaaa
      901 gttgttaaac cgccacaaaa tcctgttgct agaatagcat atacttcctt agactctaca
      961 tgattgtaga atactccaat taaaaaacaa cctaaaagat tagctatgag cgttccgagt
     1021 ggcaatttag aagcttgatt atagcgagaa aagaaatacc gtacgagtgc tcccaagcca
     1081 caggcgattg caagatagac gattaccatt tcttcctccc caaatagtaa gctaaaagca
     1141 ggcctccacc gatactcaaa agcaaataca gtatcaaact aagataacgc cctgtatcaa
     1201 gcagcttcac agtatcaagc attaggctgg aaaaagttgt taaacctccg caaaaacccg
     1261 tccccagtgc taaaatcaag cccttactag tccccttata gaccagatag cccttgacaa
     1321 gataaatcaa gcagaaaatt cccagatagt tgacaaagag agtgccccaa gggaaatctg
     1381 gactggctgg taaccaggtg gaaactagat atcggacaag tccgcccaac atagcagcta
     1441 gaaaaattcc tagcggataa aattgttctt ttttcatttg atattttgat cctgataatc
     1501 acgcgaacgt ttgagtatgt ccgaaaaagt cgcgacaata gtctcctgat agcgcttgtc
     1561 ctctacacga ctcctaacct tttcaaaaat aacttcttct ctcttagtat ctaaaatcgg
     1621 tttacctgaa gctttcttat aagcaaccac cccctcaact aaatgcattc gttcttctaa
     1681 cagtttgaca atttggtcgt cgatttgatc gatttcttga cgaataatat ctaaatccat
     1741 acagtctcct cctttatttg aattattgta tcaaaaagcc atcaattagg ctagtaaaat
     1801 cccaactctc gataagaaaa atgcactgat tgattacatc agcgcacttt tatgtttatc
     1861 ctactttagc agccaattct tctgcgaatt gttccaagcg ttcaatatct tcttcctcgg
     1921 cagaaagatc aactttaaca cactctgaac ctttttctgc tcctgttgac acaaagacac
     1981 ggtcaaagtc atcaacagcc ttacagaatt catcgtagaa ggtatctcct gagccgacca
     2041 ctccgtagat tttgccattc aagttgagat ctgctaggtc ttcgtagaag tccatcatct
     2101 catctggcaa ttcaccatca ccataagtat aggtcgcaac gatagcgatg tctgcttcca
     2161 agaagtctga agcgtcaaca gttgtacatt catcaacatc aacatccaag cccaagtcac
     2221 gtaatttgtc tgctacaata tctgcaattt cttcggtatt accggtcata ctggcaaata
     2281 caatttttgc taatgccata tcgtcctcct caatttatct ttctccatta tatcacatct
     2341 ttttcatttt aaaaataaaa attcttgtgc tacaatgaaa tgagaaagaa ttgaggttat
     2401 ttatggagat ttgccaacaa attttagaaa aaattaaaga atacgacacc attatcattc
     2461 atcgtcatat gaaaccagac cctgatgcct tgggaagtca ggtgggattg aaagccttgc
     2521 tggaacatca tttcccagaa aaaaccatca aagccgtcgg ttttgatgaa ccaactctta
     2581 cttggatggc tgagatggat cttgttgaag atagagccta ccaaggcgca cttgtcatcg
     2641 tctgtgatac agctaatact gctcgtatcg atgataagcg ctatagtcaa ggtgattttc
     2701 tcattaagat tgaccaccat ccaaatgatg atgtatacgg tgacctgtct tgggtcgata
     2761 ctagttcaag tagcgctagc gagatgatta ccctatttgc ccaaacaacc caactagcct
     2821 tggcagatcg cgatgctgag ttgctctttg caggaattgt cggtgataca ggtcgcttcc
     2881 tctacccttc taccactgca cggactcttc gcctggctgc ttatttgaga gaacataact
     2941 ttgactttgc ggctctcact cgcaaaatgg acactatgag ctacaaaatt gctaaactgc
     3001 aaggctacat ctacgaccat ctggaagtgg atgaaaatgg tgctgctcgc gttatcctga
     3061 gtcagaaaat cttgaaacaa tacaatataa ccgatgctga aactgcggcc attgtaggtg
     3121 cacctggacg cattgacaga gtgagtctct ggggaatttt tgtcgaacag gctgatggcc
     3181 actaccgagt tcgcttacgc agtaaagtcc atcctatcaa tgaaattgcc aaggagcatg
     3241 atggtggagg ccaccctcta gcaagtggtg ctaattccta tagcctagaa gaaaacgaaa
     3301 tcatctacca aaagttaaaa aacttgctta aaaactgata aaatacttgc caaacttttc
     3361 agaatctgat agactagtat agtaacaatc tatggctcgc aaagagacca tggcagaaag
     3421 gaaatattgc aaaatgaaaa aagatatcca tccagaatat cgcccagttg tcttcatgga
     3481 cacaactact ggttaccaat tccttagcgg ttcaacaaaa cgctctaacg aaacagttga
     3541 gttcgaaggc gaaacttacc cattgatccg tgtggaaatt tcatcagact cacacccatt
     3601 ctacactgga cgtcaaaagt tcactcaagc agatggacgc gtggatcgtt tcaacaaaaa
     3661 atacggtctc aaataatgat aagagaacag ttttggctgt tcttttttgt ttcttgaaat
     3721 caactgctgt tttcatgttc cagactcatc tgtaggttcg atttccatgc tactaggcag
     3781 gaaggaaata gctgtttcaa cacgtccata atgagctata ctattgtcac gaaccacact
     3841 ttcattgatg gtccaagtgg aattcatttt cttaaaagct tctcggactt tttccaaatc
     3901 tttggaggca atggcctgct ctaaggtttc aaaacgagga cttatactca tctgctttca
     3961 aaaagcattc tagtccatct ccgattaccg atggacttta tcacctcctt ctccagtcct
     4021 tgtatgacat cttgaagttg attcatgaca tcttccaaag ttcgaaaggc tttattctta
     4081 aatccacgtt tacgaatctc tttccacact tgttcaatgg gttcatctct ggtgtgtatg
     4141 gaggaataaa ggtaaaatca atattagtcg gaatatttaa ggtacttgat ttatgccata
     4201 tagcattgtc cataacgagt aaaaggataa gcttgtgaaa gctcttctaa aaaggcgttc
     4261 atccacactc ctttttataa acctgaaata aggcatcaat tgtaacaaat tctcctgcct
     4321 ctgtagcctt caaatgacgg gcaagaaagg ctttctcttc ctcaactgtc atatatgcat
     4381 ggttacgacc accacgtgtt tcttgaagga gagagtcgag tccgaactcc tcatattttt
     4441 ttacgtttcg ccaaatcgtt gtttgattac agtctaaaag ctctataatc tctttataag
     4501 atttgcccat cagacgaaat atagtagatt gaaactagaa tagtacacct ctacttctaa
     4561 aacattgtta gaaatcgatt tgtcctgttc ttgtttcatt ttactataga acgatttgaa
     4621 ggcgtttata atatttagct gtacgagagt cttttaaaag tgttttgatg gtttggattt
     4681 cttctttagt
//
LOCUS       DAVKJJ010000086         4689 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000086 DAVKJJ010000000
VERSION     DAVKJJ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.086"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            <1..1526
                     /locus_tag="VW144_001955"
     rRNA            <1..1526
                     /locus_tag="VW144_001955"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1578..1650
                     /locus_tag="VW144_001956"
     tRNA            1578..1650
                     /locus_tag="VW144_001956"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1611..1613,aa:Ala,seq:tgc)
     gene            1775..4676
                     /locus_tag="VW144_001957"
     rRNA            1775..4676
                     /locus_tag="VW144_001957"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1296 a          967 c         1399 g         1027 t
ORIGIN      
        1 ggctcaggac gaacgctggc ggcgtgccta atacatgcaa gtagaacgct gaaggaggag
       61 cttgcttctc tggatgagtt gcgaacgggt gagtaacgcg taggtaacct gcctggtagc
      121 gggggataac tattggaaac gatagctaat accgcataag agtagatgtt gcatgacatt
      181 tgcttaaaag gtgcacttgc atcactacca gatggacctg cgttgtatta gctagttggt
      241 ggggtaacgg ctcaccaagg cgacgataca tagccgacct gagagggtga tcggccacac
      301 tgggactgag acacggccca gactcctacg ggaggcagca gtagggaatc ttcggcaatg
      361 gacggaagtc tgaccgagca acgccgcgtg agtgaagaag gttttcggat cgtaaagctc
      421 tgttgtaaga gaagaacgag tgtgagagtg gaaagttcac actgtgacgg tatcttacca
      481 gaaagggacg gctaactacg tgccagcagc cgcggtaata cgtaggtccc gagcgttgtc
      541 cggatttatt gggcgtaaag cgagcgcagg cggttagata agtctgaagt taaaggctgt
      601 ggcttaacca tagtaggctt tggaaactgt ttaacttgag tgcaagaggg gagagtggaa
      661 ttccatgtgt agcggtgaaa tgcgtagata tatggaggaa caccggtggc gaaagcggct
      721 ctctggcttg taactgacgc tgaggctcga aagcgtgggg agcaaacagg attagatacc
      781 ctggtagtcc acgctgtaaa cgatgagtgc taggtgttag accctttccg gggtttagtg
      841 ccgtagctaa cgcattaagc actccgcctg gggagtacga ccgcaaggtt gaaactcaaa
      901 ggaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgaa gcaacgcgaa
      961 gaaccttacc aggtcttgac atccctctga ccgctctaga gatagagttt tccttcggga
     1021 cagaggtgac aggtggtgca tggttgtcgt cagctcgtgt cgtgagatgt tgggttaagt
     1081 cccgcaacga gcgcaacccc tattgttagt tgccatcatt tagttgggca ctctagcgag
     1141 actgccggta ataaaccgga ggaaggtggg gatgacgtca aatcatcatg ccccttatga
     1201 cctgggctac acacgtgcta caatggctgg tacaacgagt cgcaagccgg tgacggcaag
     1261 ctaatctctt aaagccagtc tcagttcgga ttgtaggctg caactcgcct acatgaagtc
     1321 ggaatcgcta gtaatcgcgg atcagcacgc cgcggtgaat acgttcccgg gccttgtaca
     1381 caccgcccgt cacaccacga gagtttgtaa cacccgaagt cggtgaggta accgtaagga
     1441 gccagccgcc taaggtggga tagatgattg gggtgaagtc gtaacaaggt agccgtatcg
     1501 gaaggtgcgg ctggatcacc tcctttctaa ggataaggaa ctgcgcattg gtcttgttta
     1561 gtcttgagag gtcttgtggg gccttagctc agctgggaga gcgcctgctt tgcacgcagg
     1621 aggtcagcgg ttcgatcccg ctaggctcca ttggtgagag atcaccaagt aatgcacatt
     1681 gaaaattgaa tatctatatc aaatagtaac aagaaaataa accgaaaacg ctgtagtatt
     1741 aataagagtt tatgactgaa aggtcagaaa ataaggttaa gttaataagg gcgcacggtg
     1801 gatgccttgg cactaggagc cgacgaagga cgtgacaaac gacgatatgc cttgggtagc
     1861 tgtaagtaag cgatgatcca gggatttccg aatgggggaa cccaacaggt aatacctgtt
     1921 acccacatct gttaaggatg tgaggaggaa gacgcagtga actgaaacat ctaagtagct
     1981 gcaggaagag aaagcaaaag cgattgcctt agtagcggcg agcgaaacgg cagaagggca
     2041 aaccgaagag tttactcttc ggggttgtag gactgcaatg tggactcaaa gattatagaa
     2101 gaatgatttg ggaagatcag ccaaagagag taatagcctc gtatttaaaa tagtctttgt
     2161 acttagcagt atcctgagta cggcgggaca cgtgaaatcc cgtcggaatc tgggaggacc
     2221 atctcccaac cctaaatact ccctagtgac cgatagtgaa ccagtaccgt gagggaaagg
     2281 tgaaaagcac cccgggaggg gagtgaaata gaacctgaaa ccgtgcgcct acaacaagtt
     2341 cgagcccgtt aatgggtgag agcgtgcctt ttgtagaatg aaccggcgag ttacgttatg
     2401 atgcgaggtt aagttgaaga gacggagccg tagggaaacc gagtctgaat agggcgcctt
     2461 agtatcatga cgtagacccg aaaccatgtg acctacccat gagcaggttg aaggtgcggt
     2521 aagacgcact ggaggaccga accagggcac gttgaaaagt gcttggatga cttgtgggta
     2581 gcggagaaat tccaaacgaa cttggagata gctggttctc tccgaaatag ctttagggct
     2641 agcgtcgaca ttagagattc ttggaggtag agcactgttt gggtgagggg tccatcccgg
     2701 attaccaatc tcagataaac tccgaatgcc aatgaattat ggtcggcagt cagactgcga
     2761 gtgctaagat ccgtagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
     2821 attgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttag cttagaagca
     2881 gctattcatt caaagagtgc gtaatagctc actagtcgag tgaccctgcg ccgaaaatgt
     2941 accggggcta aaacaattta ccgaagctgt ggataccttt ataggtatgg taggagagcg
     3001 ttctatgtgt gatgaaggta taccgtgagg agtgctggaa cgcatagaag tgagaatgcc
     3061 ggtatgagta gcgaaagaca ggtgagaatc ctgtccaccg taagactaag gtttccaggg
     3121 gaaggctcgt ccgccctggg ttagtcggga cctaaggaga gaccgaaagg tgtatccgat
     3181 ggacaacagg ttgatattcc tgtactagag tatgtagtga tggagggacg cagtaggcta
     3241 actaaagcag acgattggaa gagtctgtct aagcagtgag gtgtgaattg agtcaaatgc
     3301 ttaattctat aacattgagc tgtgatgggg agcgaagttt agtagcgaag ttagtgacgt
     3361 cacactgcca agaaaagctt ctagcgttta aacatactct acccgtaccg caaaccgaca
     3421 caggtagtcg aggcgagtag cctcaggtga gcgagagaac tctcgttaag gaactcggca
     3481 aaatgacccc gtaacttcgg gagaaggggt gctgacttaa agtcagccgc agtgaatagg
     3541 cccaagcaac tgtttatcaa aaacacagct ctctgctaaa tcgtaagatg atgtataggg
     3601 ggtgacgcct gcccggtgct ggaaggttaa gaggagtgct tagcgtaagc gaaggtatga
     3661 attgaagccc cagtaaacgg cggccgtaac tataacggtc ctaaggtagc gaaattcctt
     3721 gtcgggtaag ttccgacccg cacgaaaggc gtaatgattt gggcactgtc tcaacgagag
     3781 actcggtgaa attttagtac ctgtgaagat gcaggttacc cgcgacagga cggaaagacc
     3841 ccatggagct ttactgcagt ttgatattga gtgtctgtac cacatgtaca ggataggtag
     3901 gagtctaaga gatcgggacg ccagtttcga aggagacgct gttgggatac tacccttgtg
     3961 ttatggccac tctaacccag ataggtgatc cctatcggag acagtgtctg acgggcagtt
     4021 tgactggggc ggtcgcctcc taaaaggtaa cggaggcgcc caaaggttcc ctcagaatgg
     4081 ttggaaatca ttcgcagagt gtaaaggtat aagggagctt gactgcgaga gctacaactc
     4141 gagcagggac gaaagtcggg cttagtgatc cggtggttcc gtatggaagg gccatcgctc
     4201 aacggataaa agctaccctg gggataacag gcttatctcc cccaagagtt cacatcgacg
     4261 gggaggtttg gcacctcgat gtcggctcgt cgcatcctgg ggctgtagtc ggtcccaagg
     4321 gttgggctgt tcgcccatta aagcggcacg cgagctgggt tcagaacgtc gtgagacagt
     4381 tcggtcccta tccgtcgcgg gcgtaggaaa tttgagagga tctgctccta gtacgagagg
     4441 accagagtgg acttaccgct ggtgtaccag ttgtcttgcc aaaggcatcg ctgggtagct
     4501 atgtagggaa gggataaacg ctgaaagcat ctaagtgtga aacccacctc aagatgagat
     4561 ttcccatgat tatatatcag taagagccct gagagatgat caggtagata ggttagaagt
     4621 ggaagtgtgg cgacacatgt agcggactaa tactaatagc tcgaggactt atccaaagta
     4681 actgagaat
//
LOCUS       DAVKJJ010000087         4229 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000087 DAVKJJ010000000
VERSION     DAVKJJ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.087"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            72..>4229
                     /locus_tag="VW144_001958"
     CDS             72..>4229
                     /locus_tag="VW144_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU9601354.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPHEVVLADTSNSEDALSISDKEKV
                     VVDKETENKEKHKDIHNAIETSKDTEEKKTTIIEEKEVVSKNPVIDNNTSNEEARIKE
                     DSNQSQGDNAHSSANKNTENPKKEDKVVYIAEFKDKESGEKAIKQLSSLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPDKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLTGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNEGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQADEKG
                     MISYYLVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHHQENEESIKE
                     KSSFTIDR"
BASE COUNT         1647 a          611 c          837 g         1134 t
ORIGIN      
        1 tttataacaa aggctagcaa agttaaagtt tattatctat tggagtttaa ataaatatgt
       61 aaggaattaa aatgaaaaaa agtacagtat tgtcattaac cacagctgca gttattttag
      121 cagcctatgc ccctcatgag gtagtcttag cagacacatc taactctgaa gatgctttaa
      181 gcatctctga taaagaaaaa gtagtagtag ataaggaaac agaaaataaa gagaaacata
      241 aagatattca taatgctata gaaacttcaa aggatactga agaaaagaaa acaacaatta
      301 ttgaggaaaa agaagttgtt agtaaaaatc ctgtgataga caataacact agcaatgaag
      361 aagcaagaat caaagaagat tccaatcaat ctcaaggaga taatgcacac tcgtctgcaa
      421 ataaaaacac agaaaatccc aaaaaagaag ataaagttgt ctatattgct gaatttaaag
      481 ataaagaatc tggagaaaaa gcaatcaagc aactatccag tcttaagaat acaaaagttt
      541 tatatactta tgatagaatt tttaacggta gtgctataga aacaactcca gataacttgg
      601 acaaaattaa acaaatagaa ggtatttcat cggttgaaag ggcacaaaaa gtccaaccca
      661 tgatgaatca tgccagaaag gaaattggag ttgaggaagc tattgattac ctaaagtcta
      721 tcaatgctcc gtttgggaaa aattttgatg gtagaggtat ggtcatttca aatatcgata
      781 ctggaacaga ttatagacat aaggctatga gaatcgatga tgatgccaaa gcctcaatga
      841 gatttaaaaa agaagactta aaaggtactg ataaaaatta ttggttgagt gataaaatcc
      901 ctcatgcgtt caattattat aatggtggca aaatcactgt agaaaaatat gatgatggaa
      961 gggattattt tgacccacat gggatgcata ttgcagggat tcttgctgga aatgatactg
     1021 aacaagatat caaaaacttt aacggcatag atggaattgc acctaatgca caaattttct
     1081 cttacaaaat gtattctgac gcaggatctg ggtttgcggg tgatgaaaca atgtttcatg
     1141 ctattgaaga ttctatcaaa cacaacgttg atgttgtttc ggtatcatct ggttttacag
     1201 gaacaggtct tgtaggtgag aaatattggc aagctattag ggcattaaga aaagcaggca
     1261 ttccaatggt tgtcgctacg ggtaactatg cgacttctgc ttcaagttct tcatgggatt
     1321 tagtagcaaa taatcatctg aaaatgaccg acactggaaa tgtaacacga actgcagcac
     1381 atgaggatgc gatagcggtc gcttctgcta aaaatcaaac agttgagttt gataaagtta
     1441 acataggtgg agaaagtttt aaatacagaa atataggggc ctttttcgat aagaataaaa
     1501 tcacaacaaa tgaagatgga acaaaagctc ctagtaaatt aaaatttgta tatataggca
     1561 aggggcaaga ccaagatttg ataggtttgg atcttagggg caaaattgca gtaatggata
     1621 gaatttatac aaaggattta aaaaatgctt ttaaaaaagc tatggataag ggtgcacgcg
     1681 ccattatggt tgtaaatact gtaaattact acaatagaga taattggaca gagcttccag
     1741 ctatgggata tgaagcggat gaaggtacta aaagtcaagt gttttcaatt tcaggagatg
     1801 atggtgtaaa gctatggaac atgattaatc ctgataaaaa aactgaagtc aaaagaaata
     1861 ataaagaaga ttttaaagat aaattggagc aatactatcc aattgatatg gaaagtttta
     1921 attccaacaa accgaatgta ggtgacgaaa aagagattga ctttaagttt gcacctgaca
     1981 cagacaaaga actctataaa gaagatatca tcgttccagc aggatctaca tcttgggggc
     2041 caagaataga tttactttta aaacccgatg tttcagcacc tggtaaaaat attaaatcca
     2101 cgcttaatgt tattaatggc aaatcaactt atggctatat gtcaggaact agtatggcga
     2161 ctccaatcgt ggcagcttct actgttttga ttagaccgaa attaaaggaa atgcttgaaa
     2221 gacctgtatt gaaaaatctt acgggagatg acaaaataga tcttacaagt cttacaaaaa
     2281 ttgccctaca aaatactgcg cgacctatga tggatgcaac ttcttggaaa gaaaaaagtc
     2341 aatactttgc atcacctaga caacagggag caggcctaat taatgtggcc aatgctttga
     2401 gaaatgaagt tgtagcaact ttcaaaaaca ctgattctaa aggtttggta aactcatatg
     2461 gttccatttc tcttaaagaa ataaaaggtg ataaaaaata ctttacaatc aagcttcaca
     2521 atacatcaaa cagacctttg acttttaaag tttcagcatc agcgataact acagattctc
     2581 taactgacag attaaaactt gatgaaacat ataaagatga aaaatctcca gatggtaagc
     2641 aaattgttcc agaaattcac ccagaaaaag tcaaaggagc aaatatcaca tttgagcatg
     2701 atactttcac tataggcgca aattctagct ttgatttgaa tgcggttata aatgttggag
     2761 aggccaaaaa caaaaataaa tttgtagaat catttattca ttttgagtca gtggaagaaa
     2821 tggaagctct aaactccaac gggaagaaaa taaacttcca accttctttg tcgatgcctc
     2881 taatgggatt tgctgggaat tggaaccacg aaccaatcct tgataaatgg gcttgggaag
     2941 aagggtcaag atcaaaaaca ctgggaggtt atgatgatga tggtaaaccg aaaattccag
     3001 gaaccttaaa taagggaatt ggtggagaac atggtataga taaatttaat ccagcaggag
     3061 ttatacaaaa tagaaaagat aaaaatacaa catccctgga tcaaaatcca gaattatttg
     3121 ctttcaataa cgaagggatc aacgctccat catcaagtgg ttctaagatt gctaacattt
     3181 atcctttaga ttcaaatgga aatcctcaag atgctcaact tgaaagagga ttaacacctt
     3241 ctccacttgt attaagaagt gcagaagaag gattgatttc aatagtaaat acaaataaag
     3301 agggagaaaa tcaaagagac ttaaaagtca tttcgagaga acactttatt agaggaattt
     3361 taaattctaa aagcaatgat gcaaagggaa tcaaatcatc taaactaaaa gtttggggtg
     3421 acttgaagtg ggatggactc atctataatc ctagaggtag agaagaaaat gcaccagaaa
     3481 gtaaggataa tcaagatcct gctactaaga taagaggtca atttgaaccg attgcggaag
     3541 gtcaatattt ctataaattt aaatatagat taactaaaga ttacccatgg caggtttcct
     3601 atattcctgt aaaaattgat aacaccgccc ctaagattgt ttcggttgat ttttcaaatc
     3661 ctgaaaaaat taagttgatt acaaaggata cttatcataa ggtaaaagat cagtataaga
     3721 atgaaacgct atttgcgaga gatcaaaaag aacatcctga aaaatttgac gagattgcga
     3781 acgaagtttg gtatgctggc gccgctcttg ttaatgaaga tggagaggtt gaaaaaaatc
     3841 ttgaagtaac ttacgcaggt gagggtcaag gaagaaatag aaaacttgac aaagacggaa
     3901 ataccattta tgaaattaaa ggtgcgggag atttaagggg aaaaatcatt gaagtcattg
     3961 cattagatgg ttctagcaat ttcacaaaga ttcatagaat taaatttgct aatcaggctg
     4021 atgaaaaggg gatgatttcc tattatctag tagatcctga tcaagattca tctaaatatc
     4081 aaaagcttgg cgagattgca gaatctaaat ttaaaaattt aggaaatgga aaagagggta
     4141 gtctaaaaaa agatacaact ggggtagaac atcatcatca agaaaatgaa gagtctatta
     4201 aagaaaaatc tagttttact attgataga
//
LOCUS       DAVKJJ010000088         4196 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000088 DAVKJJ010000000
VERSION     DAVKJJ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.088"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            651..1643
                     /locus_tag="VW144_001959"
     CDS             651..1643
                     /locus_tag="VW144_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9601355.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQNNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1645..2463
                     /locus_tag="VW144_001960"
     CDS             1645..2463
                     /locus_tag="VW144_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU9601356.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2465..3250
                     /locus_tag="VW144_001961"
     CDS             2465..3250
                     /locus_tag="VW144_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9601357.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3289..3567
                     /locus_tag="VW144_001962"
                     /pseudo
     CDS             3289..3567
                     /locus_tag="VW144_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
     gene            3504..3749
                     /locus_tag="VW144_001963"
     CDS             3504..3749
                     /locus_tag="VW144_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9601358.1"
                     /translation="MSRRKKAYQGRKIGSQLLATLESEAHKKVGYLQVKTVAEGSNKD
                     YDRTNDFYRGLGFKKLEIFLQLWNPQNPCQILIKKLE"
     gene            complement(4025..>4196)
                     /locus_tag="VW144_001964"
     CDS             complement(4025..>4196)
                     /locus_tag="VW144_001964"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601359.1"
                     /translation="FEVVDRTDEVSSKHCFEVVDRTDEVSNHTHGKATLTWFEEIFEE
                     YKSKTFVNRDTL"
BASE COUNT         1162 a          726 c          905 g         1403 t
ORIGIN      
        1 gaatatgaaa gctttttata tcactattga aaaatttata cgagatgatg aaagccttaa
       61 gtgttatttt acaaaggtta tttcaagtcg ttccaaggta acaagtctag atcagattga
      121 agctgataaa acgatacaaa gaaaatattc aagtgagcta aaaaaattta ttggatttta
      181 taatgagatt atttgtgagg aaaatagttt cctacatgta cgaaagaggt ggtcgagttg
      241 gtttaggtag tcgatgcgtg agttgataat tctcagggta tggacttctt tttcatgaat
      301 gaggtaaaag agcaggtatt gtttagagac aatcattctg agcatatttt ctggatagag
      361 ggagtatccg attttatgat caaagttaat accgccctct ggtgagaaga tgagtaggtt
      421 ggtaatttaa actattaaac agaatttttg attaaaagta ttattttatg agagaaatcc
      481 taatttcaca atccataggc aaacgcttgc attctgtttt ttattggact ataataggtt
      541 ggtataaagc cttctgtagt aataaaatgt agaaggtgta gaaagtaagg atttagaata
      601 tttgtagtta aaaatacaat gttgctattc cttatgatag ggagatagat atggcaatga
      661 tagaagtgga acatcttcag aaaaattttg tgaagactgt taaggaaccg ggcttgaagg
      721 gggctttgcg ctcctttatt catcctgaaa agcagacctt tgaagcggtc aaggatttga
      781 cctttgaggt tccaaaaggg cagattttag gatttatcgg ggcaaatggt gctgggaagt
      841 cgacaaccat taaaatgctg acaggaattt tgaaaccaac atctggtttt tgtcggatta
      901 acggcaagat tccccagaac aatcggcaag attatgtcaa agatattggc gtagtctttg
      961 gacaacgcac ccagctatgg tgggatttgg ctctgcaaga gacctacact gtcttaaaag
     1021 agatttatga tgtgccagac tcgctctttc ataagcgtat ggactttttg aatgaagtct
     1081 tggatttgaa ggactttatc aaggatcccg tgcgaactct ttcactggga caacggatgc
     1141 gggcggatat tgcggcctcc ttgctccaca atcccaaggt tcttttttta gatgagccga
     1201 ccattggttt ggacgtttcg gttaaggata atattcgtcg ggcaattact cagatcaatc
     1261 aagaggaaga aactaccatt cttttgacca ctcatgattt gagtgatatt gagcaacttt
     1321 gtgatcggat tttcatgatt gacaaggggc aagagatttt tgatggaacg gtgagccaac
     1381 tcaaggagac ctttggtaag atgaagactc tctcttttga actgctacca ggtcaaagtc
     1441 atctcgtctc tcactatgaa ggtctgtctg atatgaccat tgatagacaa ggaaacagcc
     1501 tcaacattga atttgatagt tctcgctacc agtcagctga cattatcaag caaaccctgt
     1561 ctgattttga aatccgcgat ttgaagatgg tggatacgga tattgaggat attatccgtc
     1621 gcttctaccg aaaggagctc taggatgatc aaattgtgga gacgttataa accctttatc
     1681 aatgcagggg ttcaggagtt gattacttac cgagtcaact ttattctcta tcggattggc
     1741 gatgtcatgg gggcttttgt ggccttttat ctctggaagg ctgtctttga ttcttcgcaa
     1801 gagtctttga ttcagggctt cagtatggcg gatatcaccc tctacatcat catgagtttt
     1861 gtgaccaatc ttctgactag atccgattcg tcctttatga ttggggagga ggtcaaggat
     1921 ggctccatta tcatgcgttt gttgcgacca gtgcattttg cggcctccta tcttttcacc
     1981 gagcttggtt ccaagtggtt gatttttatc agcgttggcc ttccattttt aagtgtcatt
     2041 gtcttgatga aaatcatatc gggtcaaggt attgtagagg tgctaggatt aactgtcctt
     2101 tatcttttta gcttaacgct cgcctatctg attaactttt tctttaatat ttgctttgga
     2161 ttttcagcct ttgtgtttaa aaatctttgg ggttccaacc tacttaagac ttccatagtg
     2221 gcttttatgt cggggagttt gattcccttg gcattctttc caaaggttgt ttcagatatt
     2281 ctctcctttt tgcctttttc atccttgatt tatactccag ttatgatcat tgttggaaaa
     2341 tacgatgcca gtcagattct tcaggcactc cttttgcagt tcttctggct cttagtgatg
     2401 gtgggattgt ctcagttgat ttggaaacgg gtccagtcct ttatcaccat tcaaggaggt
     2461 tagtatgaaa aaatatcaac gaatgcatct gatttttatc agacaataca tcaaacaaat
     2521 catggaatat aaggtggatt ttgtggttgg tgttttggga gtctttctgg ctcaaggctt
     2581 gaatctcttg tttctcaatg tcatctttca acatattcca ttcctagaag gctggacctt
     2641 tcaagagata gctttcattt atggattttc cttgattccc aagggaatgg accatctctt
     2701 ttttgacaat ctctgggcac tagggcaacg cctagtccga aaaggggagt ttgacaagta
     2761 tctgactcgt cccatcaatc ctctctttca catcctagtt gaaacctttc agattgatgc
     2821 cttgggtgaa ctcttagtcg gtggtatttt attgggaaca acagtgacca gcattgtttg
     2881 gactcttcca aaattcctgc ttttcctagt ttgtattcct tttgcgacct tgatttatac
     2941 ttctcttaaa atcgcaacag ccagtatcgc cttttggact aagcagtcag gcgccatgat
     3001 ttacatcttc tatatgttca atgactttgc taagtatccg atttctattt acaattctct
     3061 tcttcgttgg ttgattagct ttatcgtgcc tttcgccttt acagcctact atccagctag
     3121 ctatttctta caggaaaagg atgtgttctt taacgtagga ggtttgatgt tgatttctct
     3181 ggttttcttt gttatttccc ttaaactttg ggataagggc ttagattcct acgaaagtgc
     3241 gggttcgtaa aagctaaagt aagactaaaa tcaagaaagg aacttatgat gtttgtaatt
     3301 gaagaagtca aggatgaaaa tcaaaaaaag gcagttgtcg ctgaggtttt gaaggatttg
     3361 ccagaatggt ttggaatccc agaaagcaca taagcctata taaaaggaac cacgacactg
     3421 caagtttgga ccgcctatca ggagagtgat ttgactggat ttgtaagctt atcctattcg
     3481 agtgaagatt gtgcagagat tgattgtctc ggcgtaaaaa agcttatcaa ggtagaaaaa
     3541 ttgggagcca attgcttgct actttagaga gtgaagctca taaaaaagtt ggttatctgc
     3601 aggtcaaaac agtggcagaa ggttctaata aagattatga tcgaacaaat gacttttatc
     3661 gaggtcttgg ctttaaaaag ttagagattt ttcttcaact atggaatccg caaaatcctt
     3721 gtcagatttt gattaaaaag cttgaataat attacttgac atctattctc agagtgctat
     3781 actgtaagtg taatcgccga tttagcttag ttggtagagc aaggcactcg taaagcctag
     3841 gttataggta gataaacgac tgaggatttg aaaaaataga taggtagaag ataaccgtta
     3901 agccttactc ttagcggtta tttatattgt ttaatagcgc taatatttta tcaattatgc
     3961 ctgttttcgt gtttctggta gttgttcaag tttattgcta atatttttga tggtatgaat
     4021 gtgcttataa tgtatcccgg ttaacgaaag ttttggactt atactcttcg aaaatctctt
     4081 caaaccacgt caacgtcgcc ttgccgtgcg tatggttact gacttcgtca gttctatcca
     4141 caacctcaaa acagtgtttt gagctgactt cgtcagttct atctacaacc tcaaaa
//
LOCUS       DAVKJJ010000089         4190 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000089 DAVKJJ010000000
VERSION     DAVKJJ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.089"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            <1..736
                     /locus_tag="VW144_001965"
     CDS             <1..736
                     /locus_tag="VW144_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU9601360.1"
                     /translation="HQDSGNTEAKGAEAIYNRVKAAKKVPLDRMPYNLQYTVEVKNGS
                     LIIPHYDHYHNIKFEWFDEGLYEAPKGYSLEDLLATVKYYVEHPNERPHSDNGFGNAS
                     DHVRKNKADQDSKPDEDKGHDEVSEPTHPESDEKENHAGLNPSADNLYKPSTDTEETE
                     EEAEDTTDEAEIPQVEHSVINAKIADAEALLEKVTDPSIRQNAMETLTGLKSSLLLGT
                     KDNNTISAEVDSLLALLKKSQPAPIQ"
     gene            946..4065
                     /gene="phtE"
                     /locus_tag="VW144_001966"
     CDS             946..4065
                     /gene="phtE"
                     /locus_tag="VW144_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEU9601361.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1552 a          698 c          822 g         1118 t
ORIGIN      
        1 ccatcaggat tcaggaaata ctgaggcaaa aggagcagaa gctatctaca accgcgtgaa
       61 agcagctaag aaggtgccac ttgatcgtat gccttacaat cttcaatata ctgtagaagt
      121 caaaaacggt agtttaatca tacctcatta tgaccattac cataacatca aatttgagtg
      181 gtttgacgaa ggcctttatg aggcacctaa ggggtatagt cttgaggatc ttttggcgac
      241 tgtcaagtac tatgtcgaac atccaaacga acgtccgcat tcagataatg gttttggtaa
      301 cgctagcgac catgttcgta aaaataaggc agaccaagat agtaaacctg atgaagataa
      361 gggacatgat gaagtaagtg agccaactca ccctgaatct gatgaaaaag agaatcacgc
      421 tggtttaaat ccttcagcag ataatcttta taaaccaagc actgatacgg aagagacaga
      481 ggaagaagct gaagatacta cagatgaggc tgaaattcct caagtagagc attctgttat
      541 taacgctaag atagcagatg cggaggcctt gctagaaaaa gtaacagatc ctagtattag
      601 acaaaatgct atggagacat tgactggtct aaaaagtagt cttcttctcg gaacgaaaga
      661 taataacact atttcagcag aagtagatag tctcttggct ttgttaaaaa aaagtcaacc
      721 ggctcctata cagtagtaaa atgaatggag catattttat ggagaagtaa cctttcgtgt
      781 tacttctctt ttttagaaaa acgtaacaga aacttgacaa aagtaatttt aaatagtaaa
      841 ctatttactg gttaattaaa tggttaaata accggtttag aaaactattt aataaagtaa
      901 aagaagttga gaaaaaactt catcatttat tgaaatgagg gatttatgaa attcagtaaa
      961 aaatatatag cagctggatc agctgttatc gtatccttga gtctatgtgc ctatgcacta
     1021 aaccagcatc gttcgcagga aaataaggac aataatcgtg tctcttatgt ggatggcagc
     1081 cagtcaagtc agaaaagtga aaacttgaca ccagaccagg ttagccagaa agaaggaatt
     1141 caggccgagc aaattgtaat caaaattaca gatcagggct atgtaacgtc acacggtgac
     1201 cactatcatt actataatgg gaaagttcct tatgatgccc tctttagtga agaactcttg
     1261 atgaaggatc caaactatca acttaaagac gctgatattg tcaatgaagt caagggtggt
     1321 tatatcatca aggtcgatgg aaaatattat gtctacctga aagatgcagc tcatgctgat
     1381 aatgttcgaa ctaaagatga aatcaatcgt caaaaacaag aacatgtcaa agataatgag
     1441 aaggttaact ctaatgttgc tgtagcaagg tctcagggac gatatacgac aaatgatggt
     1501 tatgtcttta atccagctga tattatcgaa gatacgggta atgcttatat cgttcctcat
     1561 ggaggtcact atcactacat tcccaaaagc gatttatctg ctagtgaatt agcagcagct
     1621 aaagcacatc tggctggaaa aaatatgcaa ccgagtcagt taagctattc ttcaacagct
     1681 agtgacaata acacgcaatc tgtagcaaaa ggatcaacta gcaagccagc aaataaatct
     1741 gaaaatctcc agagtctttt gaaggaactc tatgattcac ctagcgccca acgttacagt
     1801 gaatcagatg gcctggtctt tgaccctgct aagattatca gtcgtacacc aaatggagtt
     1861 gcgattccgc atggcgacca ttaccacttt attccttaca gcaagctttc tgccttagaa
     1921 gaaaagattg ccagaatggt gcctatcagt ggaactggtt ctacagtttc tacaaatgca
     1981 aaacctaatg aagtagtgtc tagtctaggc agtctttcaa gcaatccttc ttctttaacg
     2041 acaagtaagg agctctcttc agcatctgat ggttatattt ttaatccaaa agatatcgtt
     2101 gaagaaacgg ctacagctta tattgtaaga catggtgatc atttccatta cattccaaaa
     2161 tcaaatcaaa ttgggcaacc gactcttcca aacaatagtc tagcaacacc ttctccatct
     2221 cttccaatca atccaggaac ttcacatgag aaacatgaag aagatggata cggatttgat
     2281 gctaatcgta ttatcgctga agatgaatca ggttttgtca tgagtcacgg agaccacaat
     2341 cattatttct tcaagaagga cttgacagaa gagcaaatta aggctgcgca aaaacattta
     2401 gaggaagtta aaactagtca taatggatta gattctttgt catctcatga acaggattat
     2461 ccaagtaatg ccaaagaaat gaaagattta gataaaaaaa tcgaagaaaa aattgctggc
     2521 attatgaaac aatatggtgt caaacgtgaa agtattgtcg tgaataaaga aaaaaatgcg
     2581 attatttatc cgcatggaga tcaccatcat gcagatccga ttgatgaaca taaaccggtt
     2641 ggaattggtc attctcacag taactatgaa ctgtttaaac ccgaagaagg agttgctaaa
     2701 aaagaaggga ataaagttta tactggagaa gaattaacga atgttgttaa tttgttaaaa
     2761 aatagtacgt ttaataatca aaactttact ctagccaatg gtcaaaaacg cgtttctttt
     2821 agttttccgc ctgaattgga gaaaaaatta ggtatcaata tgctagtaaa attaataaca
     2881 ccagatggaa aagtattgga gaaagtatct ggtaaagtat ttggagaagg agtagggaat
     2941 attgcaaact ttgaattaga tcaaccttat ttaccaggac aaacatttaa gtatactatc
     3001 gcttcaaaag attatccaga agtaagttat gatggtacat ttacagttcc aacctcttta
     3061 gcttacaaaa tggccagtca aacgattttc tatcctttcc atgcagggga tacttattta
     3121 agagtgaacc ctcaatttgc agtgcctaaa ggaactgatg ctttagtcag agtgtttgat
     3181 gaatttcatg gaaatgctta tttagaaaat aactataaag ttggtgaaat caaattaccg
     3241 attccgaaat taaaccaagg aacaaccaga acggccggaa ataaaattcc tgtaaccttc
     3301 atggcaaatg cttatttgga caatcaatcg acttatattg tggaagtacc tatcttggaa
     3361 aaagaaaatc aaactgataa accaagtatt ctaccacaat ttaaaaggaa taaagcacaa
     3421 gaaaactcaa aacttgatga aaaggtagaa gaaccaaaga ctagtgagaa ggtagaaaaa
     3481 gaaaaacttt ctgaaactgg gaatagtact agtaattcaa cgttagaaga agttcctaca
     3541 gtggatcctg tacaagaaaa agtagcaaaa tttgctgaaa gttatgggat gaagctagaa
     3601 aatgtcttgt ttaatatgga cggaacaatt gaattatatt taccatcggg agaagtcatt
     3661 aaaaagaata tggcagattt tacaggagaa gcacctcaag gaaatggtga aaataaacca
     3721 tctgaaaatg gaaaagtatc tactggaaca gttgagaacc aaccaacaga aaataaacca
     3781 gcagattctt taccagaggc accaaacgaa aaacctgtaa aaccagaaaa ctcaacggat
     3841 aatggaatgt tgaatccaga agggaatgtg gggagtgacc ctatgttaga tccagcatta
     3901 gaggaagctc cagcagtaga tcctgtacaa gaaaaattag aaaaatttac agctagttac
     3961 ggattaggct tagatagtgt tatattcaat atggatggaa cgattgaatt aagattgcca
     4021 agtggagaag tgataaaaaa gaatttatct gatctcatag cgtaaggaat agcagtagaa
     4081 aaagtctgaa tcaaaaatga agttctctca aaagttagaa ataaaactct gactttggga
     4141 gaatttcatt ttattattaa tatataaaat ttcttgacat acaacttaaa
//
LOCUS       DAVKJJ010000090         3743 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000090 DAVKJJ010000000
VERSION     DAVKJJ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.090"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            158..385
                     /locus_tag="VW144_001967"
     CDS             158..385
                     /locus_tag="VW144_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601362.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            673..3570
                     /gene="nanA"
                     /locus_tag="VW144_001968"
     CDS             673..3570
                     /gene="nanA"
                     /locus_tag="VW144_001968"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEU9601363.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPNGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKEIDGKTYQILY
                     REGEKEAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGDQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NKRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWDFLSKNLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGIEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1286 a          617 c          845 g          995 t
ORIGIN      
        1 gttttaaaaa taaggtataa taaatttact aacacttata aaaagtgata gaatctatct
       61 ttatgtatat ttaaagatag attgctgtaa aaatagtagt agctatgcga aataacagat
      121 agagagaagg gattgaagct tagaaaaggg gaataatatg atatttaagg cattcaagac
      181 aaaaaagcag agaaaaagac aagttgaact acttttgaca gtttttttcg acagttttct
      241 gattgattta tttcttcact tatttgggat tgtccccttt aagctggata agattctgat
      301 tgtgagcttg attatatttc ccattatttc tacaagtatt tatgcttatg aaaagctatt
      361 tgaaaaagtg ttcgataagg attgagcagg gagtatggta taaatagcat aagctgatgt
      421 ccatcatttg cttataaaga gatattttag tttaattgca gcggtgtcct ggtagataaa
      481 ctagattggc aggagtctga ttggagaaag gagaggggaa aattggcacc aatttgagat
      541 agtttgttta gttcattttt gtcatttaaa tgaactgtag taaaagaaag ttaataaaag
      601 acaaactaag tgcattttct ggagtaaatg tcttatttca gaaatcggga tatagatata
      661 gagaggatca gtatgaatcg gagtgttcaa gaacgtaagt gtcgttatag cattaggaaa
      721 ctatcggtag gagcggtttc tatgattgta ggagcagtgg tatttggaac gtctcctgtt
      781 ttagctcaag aaggggcaag tgagcaacct ctggcaaatg aaactcaact ttcgggggag
      841 agctcaaccc taactgatac agaaaagagc cagccttctt cagagactga actttctggc
      901 aataagcaag aacaagaaag gaaagataag caagaagaaa aaattccaag agattactat
      961 gcacgagatt tggaaaatgt cgaaacagtg atagaaaaag aagatgttga aaccaatgct
     1021 tcaaatggtc agagagttga tttatcaagt gaactagata aactaaagaa acttgaaaac
     1081 gcaacagttc acatggagtt taagccagat gccaaggctc cagcattcta taatctcttt
     1141 tctgtgtcaa gtgctactaa aaaagatgag tacttcacta tggcagttta caataatact
     1201 gctactctag aggggcgtgg ttcggatggg aaacagtttt acaataatta caacgatgca
     1261 cccttaaaag ttaaaccagg tcagtggaat tctgtgactt tcacagttga aaaaccgaca
     1321 gcagaattgc caaatggtcg agttcgcctg tatgttaatg gtgttttatc tcgaacaagt
     1381 ctgaaatctg gcaatttcat taaagatatg ccagatgtaa cgcatgtgca aatcggagca
     1441 accaagcgtg ccaacaatac ggtttggggg tcaaatctac agattaggaa tctcactgtg
     1501 tataatcgtg ctttaacacc agaagaggta caaaaacgta gtcaactttt taaacgctca
     1561 gatttagaaa aaaaactacc tgaaggagcg gctttaacag agaaaacgga catattcgaa
     1621 agcgggcgta acggtaaacc aaataaagat ggaatcaaga gttatcgtat tccagcactt
     1681 ctcaagacag ataaaggaac tttgatcgca ggtgcagatg aacgccgtct ccattcgagt
     1741 gactggggtg atatcggtat ggtcatcaga cgtagtgaag ataatggtaa aacttggggt
     1801 gaccgagtaa ccattaccaa cttacgtgac aatccaaaag cttctgaccc atcgatcggt
     1861 tcaccagtga atatcgatat ggtgttggtt caagatcctg aaaccaaacg aatcttttcc
     1921 atctatgaca tgttcccaga agggaaggga atctttggaa tgtcttcaca aaaagaagaa
     1981 gcctacaaag aaatcgatgg aaaaacctat caaatcctct atcgtgaggg agaaaaggaa
     2041 gcttatacca ttcgagaaaa tggtactgtc tatacaccag atggtaaggc gacagactat
     2101 cgcgttgttg tagatcctgt taaaccagcc tatagcgaca agggtgatct atacaagggt
     2161 gaccaattac taggaaatat ctacttcaca acaaacaaaa cttctccatt tagaattgcc
     2221 aaggatagct atctatggat gtcctacagt gatgacgacg ggaagacatg gtcagcgcct
     2281 caagatatta ctccgatggt caaagccgat tggatgaaat tcttgggtgt aggtcctgga
     2341 acaggaattg tacttcggaa tgggcctcac aagggacgga ttttgatacc ggtttatacg
     2401 actaataatg tatctcactt aaatggctcg caatcttctc gtgtcatcta ttcagatgat
     2461 catggaaaaa cttggcatgc tggagaagcg gtcaacgata accgtcaggt agacggtcaa
     2521 aagatccact cttctacgat gaacaataaa cgtgcgcaaa atacagaatc aacggtggta
     2581 caactaaaca atggagatgt taaactcttt atgcgtggtt tgactggaga tcttcaggtt
     2641 gctacaagta aagacggagg agtgacttgg gagaaggata tcaaacgtta tccacaggtt
     2701 aaagatgtct atgttcaaat gtctgctatc catacgatgc acgaaggaaa agaatacatt
     2761 atcctcagca atgcaggtgg accgaaacgt gaaaatggga tggtccactt ggcacgtgtc
     2821 gaagaaaatg gtgagttgac ttggctcaaa cacaatccaa ttcaaaaagg agagtttgcc
     2881 tataattcgc tccaagaatt aggaaatggg gagtatggta tcttgtatga acatactgaa
     2941 aaaggacaaa atgcctatac cctatcattt agaaaattta attgggactt tttgagcaaa
     3001 aatctgattt ctcctaccga agcgaaagtg aagcgaacta gagagatggg caaaggagag
     3061 atgggcaaag gagttattgg cttggagttc gactcagaag tattggtcaa caaggctcca
     3121 acacttcaat tggcaaatgg taaaacagcg actttcctaa cccagtatga tagcaagacc
     3181 ttgttgtttg cagtagataa ggaagatatc ggacaagaaa ttattggtat agctaaagga
     3241 agcatcgaaa gtatgcataa tcttcctgta aatctagcag gtgccagagt tcctggcgga
     3301 gtaaatggta gcaaagcagc ggtgcatgaa gttccagaat ttacaggggg agttaatggt
     3361 atagagccag ctgttcatga aatcgcagag tataagggat ctgattcgct tgtaactctt
     3421 actacaaaag aagattatac ttacaaagct cctcttgctc agcaggcact tcctgaaaca
     3481 ggaaacaagg agagtgacct cctagcttca ctaggactaa cagctttctt ccttggtctg
     3541 tttacgctag ggaaaaagag agaacaataa gagaagaatt ctaaacattt gattttgtaa
     3601 aaatagaagg agatagcagg ttttcaagcc tgctatcttt ttttgatgac attcaggctg
     3661 atacgaaatc ataagaggtc tgaaactact ttcagagtag tctgttctat aaaatatagt
     3721 agattgaaat aagatgtgaa caa
//
LOCUS       DAVKJJ010000091         3415 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000091 DAVKJJ010000000
VERSION     DAVKJJ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.091"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            21..560
                     /locus_tag="VW144_001969"
                     /pseudo
     CDS             21..560
                     /locus_tag="VW144_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037046.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            665..1013
                     /locus_tag="VW144_001970"
                     /pseudo
     CDS             665..1013
                     /locus_tag="VW144_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(1284..1505)
                     /locus_tag="VW144_001971"
     CDS             complement(1284..1505)
                     /locus_tag="VW144_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601364.1"
                     /translation="MDLFQELICLVQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            1470..2099
                     /locus_tag="VW144_001972"
     CDS             1470..2099
                     /locus_tag="VW144_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601365.1"
                     /translation="MNKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSANSKDKTALLESTKAILVLNHQIQSKVSASEETSPARNAEANSDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            complement(2135..3358)
                     /gene="pepT"
                     /locus_tag="VW144_001973"
     CDS             complement(2135..3358)
                     /gene="pepT"
                     /locus_tag="VW144_001973"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEU9601366.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPRQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
BASE COUNT         1143 a          651 c          657 g          964 t
ORIGIN      
        1 tgaaatttaa aaagaaatat atagcagctg gatctgttgt tatcctttcc ttaagtctgt
       61 gtgtttatgc tctgaaccaa catagctaac aggccaatac agataaaaat cgtgtttcat
      121 atgtaaacag taataaagac actaagaaga ctgaaaattt gactccagac taggttagtc
      181 aaaaagaagg aattcaagca aaacaaattg ttattaagat tacagatcaa ggttatgtaa
      241 cttctcatgg cgatcattat cactactata atggtaaagt tccttttgat gcgctcttta
      301 gtgaagagcc attaatgaaa gcccccaact atcagccgaa agatcaagat attgtcagtt
      361 aggttaaggg aggatatatt attaaggtag atggaaagta ttatgtttac cttaaagatc
      421 aagctcatgc agaaaatgta cgaacaaaag atgaaatcaa tcgccaaaaa caagaacatg
      481 gtaaagacga taaaggagct agtgcagaag tatctgtagc aaaattacag ggacgttata
      541 ctaccgatga tggttatata tagtgagttg aatttagaat agcacatcat agtttctaaa
      601 tcattgttag aaattacttt aaaatcccct tatcaatttg ctcatattct atttcaatct
      661 actaatagta ccacatggtg gtcactatca tttcattcct gagagtgact tgtcggcagg
      721 tgaattagca gccacatatg tctttaaccc taatgatatt gtcagagata taggtgatgc
      781 ttatatagta agacatgggg aacactatca ctatattcct aagtcatctc ttaacaatcc
      841 cccatcacac tctaacacgg aagaggtagg aagttcttct agttctgtat taagtaatcc
      901 gtcactacat gtccaccatg aggaagaaga tggttatggt ttttgatgcc aatcgaatta
      961 taagtgaaga ttcagaaggt tttgtgattc ctcatggcga ccacaatcac tacatcaagg
     1021 ttcaaactaa gggatatgaa gcggctttga aaaacaagat tccatccctg caatccaact
     1081 atcaacctgg aacttttgat gagaaggcag tcttggcaaa agtagatcaa cttctagctg
     1141 atagcagaag catctacaaa gacagattga gttagcttta ggtcagttta ctgaaagttt
     1201 gaaaaaaata aaagttagtt gattgattac ttctctaaat tttgggcaca ctaaaaagag
     1261 gtcttgacac ctaaacttta aaattaaagc atattgaatc cgtgctgctg tgcgaagcga
     1321 atagcgtctt ggacgaacat tgtcttatct ggatttctta tttctgtaag gccttgtaat
     1381 cttggattat aactctcctg ttttaggagt acatggtaga tagcaaccaa taatctgcga
     1441 cagatagcaa ggaagaagta gaaagtcttt tgaacaaggc aaatcaactc ttggaacaaa
     1501 tccatgaaga aggaatcaga caatccttgg cagaagaagt agaaaatcta aaagctgcca
     1561 caaacaaggc tgatgcagac ttggatgaag taaacagtca ggtaaaagat gttttgactc
     1621 gtatcgctag cgcccttcaa caagaaaagg aaaatgctga gcaagattct cagacacttg
     1681 tactctatca aaaactctac gatattctca tgtcgcttca ctcttattta gaaagtaata
     1741 atggatctga tgcggacttt gataaggtgg atgcattatt agatcaactt tctgctaata
     1801 gtaaagacaa aacagcttta cttgaatcga caaaagctat tctagttttg aatcatcaaa
     1861 tccagtcaaa agttagcgca agtgaagaaa caagtccagc aagaaatgct gaagcaaata
     1921 gtgataacac cagtgctgag aaccaaccaa acgcaactgc agaatctaac attgaaactg
     1981 ctagcgacga aaacaaacca agcaatacaa gagattctaa acctgctgaa tcaacttcag
     2041 aaaatgaaac aacagaatct tctacaacta ctggaaatca agaaaaacca gtagaataaa
     2101 aagaaaggcg aagtagctga gccacctcgt ctttttagcc tttataagct acgatgccaa
     2161 tgatggtatc aactgcacgt tccatagtct gaaggctaac gtattcaaaa cgtccgtgca
     2221 tattttcgcc acctgcaaag atattcggag ttgggattcc cataaaggaa atcttagagc
     2281 cgtctgttcc accccggatt ggttcgataa taggcgtgat acctagatct tccataacgg
     2341 ctttagcaat ggtaattgga gtcatatctt tttcaatgac ttctttcata ttgtagtact
     2401 ggtctgtcaa gttgagagtg acacggtcgc tcccaagttc ttcattcatc ttatcagcga
     2461 tagattgcat ggatgcttta cgcgcttcaa aggcatcttt ttcaaaatca cgaatgatgt
     2521 agcttgcacg cacctcctca acactacctg tcacatccat tagatggtaa aaaccttggt
     2581 aaccttcagt taactcaggt cggtcatttt ctggaagttg attatgaaaa tcaattgcta
     2641 gctgaagggc attgaccatc tgccctttgg cagtaccagg gtggacatta cggccttgga
     2701 aatgcaattc agcaccagcg gctgagaaag tctcgtactg aagttcacct agtggaccac
     2761 catcaacagt gtaggcaaaa tccacatcaa aatcttctgc atcaaattta ttggcaccaa
     2821 caccgatttc ttcatctgga ccaaaaccaa cacgaatctc acagtgctta atttcaggat
     2881 gagcagttag atattcaatg gctgtcataa tttcagcaat tcctgacttg tcatcagcac
     2941 ctagcaaggt tgttccatct gttgtgatga gtgtttgtct tggatatttt tcaagactct
     3001 tgaagtcggc tggatcgagt ttgaaaccag aattccctag ttcaatcaca ccaccatcgt
     3061 agttttcaat tacctgtgga ttgactcctt cagcattaaa atcagcagta tccatgtgcg
     3121 atataaaacc aatcttacgt gttaaagacg gatcgttggc tggcaaggtt ccaatagcaa
     3181 aaccattcgg tagatagtaa acattttgca gtccaacacg tttcatttca ggaattagga
     3241 catttgttgc gaagtcaacc tgactctgtg tacttggagt agtagtagag tgttcatcag
     3301 agcgcgtgtt gaccttaacg taggttaaga agcggtccaa gagatttgga taagtcataa
     3361 aaaaactcct tttttatcat aaaatagaat acaaaacttc ggaaaaatat gattt
//
LOCUS       DAVKJJ010000092         3342 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000092 DAVKJJ010000000
VERSION     DAVKJJ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.092"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(251..862)
                     /gene="rpsD"
                     /locus_tag="VW144_001974"
     CDS             complement(251..862)
                     /gene="rpsD"
                     /locus_tag="VW144_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU9601367.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1057..2109)
                     /locus_tag="VW144_001975"
     CDS             complement(1057..2109)
                     /locus_tag="VW144_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9601368.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2106..2804)
                     /locus_tag="VW144_001976"
     CDS             complement(2106..2804)
                     /locus_tag="VW144_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9601369.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2971..>3342)
                     /locus_tag="VW144_001977"
     CDS             complement(2971..>3342)
                     /locus_tag="VW144_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9601370.1"
                     /translation="LDNIVATKKVTIKINVKETSDTANGSLSPSNSGSGVTPMNHNHA
                     TGTTDSMPADTMTSSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGF
                     LGLALAGLLGGLGLKNKKEEN"
BASE COUNT          935 a          748 c          638 g         1021 t
ORIGIN      
        1 tgttatacta cctgttcgct cacaataaga gagaactttt ttacgaaaat ctattgaata
       61 ttccataaaa gattatacca cattgtgtac tatttttggt tcattttact atatcacttt
      121 ggactatatc ggtctagtta tgtctaaacg attatagctg gatactaaga gaaatcaaaa
      181 aagcactaag ggagaacgtc ccaaagtgct taatatcaag gcctcaaagc ctgtcttatt
      241 caataaaata ttacaacatc ttgttgtaga attcaacgac aagtgcttcg ttgatttctg
      301 ggttgatttc gtcgcgttct ggcaagcgag tcaatgaacc ttccaatttt tcagcgtcga
      361 atgatacgaa tgctggacgt ccaagagtag cttctactgc ttcaaggatt gctggaactt
      421 tcaatgattt ttcacgaact gagatcactt gacctggagt tacgcggtat gatgggatat
      481 caacgcgttt cccgtcaaca aggatgtgac cgtggtttac gaattgacga gcttgacgac
      541 gagtagtcgc gagaccaaga cggtaaacaa cgttatccaa acgacgttcc aaaagaagca
      601 taaagttgaa acctaggatt ccgcctttga tttttgtagc ttgtacgaac aagttacgga
      661 attgtttttc acctacaccg taagtgaaac gaagtttttg tttttcagcc aattgcaaac
      721 cgtattctga caatttagaa cggttgtttg gtccgtgttg tcctggtacg tagttacgac
      781 gtgccaattc tttacctgta cctgtaagtg aaaggccaag gcgacgagct tgtttccaag
      841 atggtcctgt ataacgtgac atatgaatgt cctcctgata taaataatat ttcggtggaa
      901 atagtcactt agaaagccct gattcgtgca gatgcccttc gcctaaacag ccaaggttac
      961 ttgtcataag acacctgttg acgagcttca tgctttcctg ctgatatttc acacaaaatc
     1021 tattctacca tgaatagctg gatttgtaaa ggggttttag gctttatttt cactaccaga
     1081 gaggttgaga acgagggtaa aggtacttcc tagaccgtac tggctgctga ctgtgatttc
     1141 cccacccaat tgatgggcca attcacgcgc aatcgcaagt cctaatccat gaccacctgt
     1201 cttcatgtta cgcgaagttt cgacacgata aaggcgtttg aaaatatttt ccaaatcctc
     1261 tggggcaata ccctgccctt catcggtcac actgattgaa agctggtcct tctccagctt
     1321 agccaccact tccagcttgg ttcctggagc agaatattta aaagcgttat cgaccagatt
     1381 caccaagata cgagaaagct tagcataatc tccctcaatc cgggcagact ctgggattac
     1441 ctgcaagtgg acatctcttc tctcctgctc aatcaaaaac tgaaattcac tcatgcactc
     1501 aattaagagc ttgtccagaa aaatactgtc tttactggta gtttccacct gatttctagc
     1561 tgtgtttagg gtcaaaaaat tcaactcctc aaccagttta ttgagcctct ccgtctggcg
     1621 tccaatggtt gctagataat gagcttgctc cgactccttg ataataccat ccaaaatccc
     1681 ttctaccgtc gcttggatcg aagtgatagg agtcttaata tcatgcgaca actgggcaat
     1741 catcaagccc ttttctcgtt cgctttcttc caaggaatca aagcttacct gcaaatcatg
     1801 ggacatctca ttaaaagttt gccctaattg ctgaaattct acaggacctt gaacctccaa
     1861 atttgaagga aaatccttgg ccgctacccg cttggcatgc tccttgagtt tgcccaacga
     1921 cgtaaagact ggcaatagga gaaagagact aatcccagca ccgacaaggc tggcaacgat
     1981 ggtcatccca agcaaaaagt aaatctcgcc tttcgcaatc agcatttttt gaacagccca
     2041 aaaaacaacc aaaatggtta agagggttga aataatatat ccaaccaaaa tataactttt
     2101 tagtttcatg tttgtcctct cggtttctct atcttatatc ccaaccccca aactgtctta
     2161 atagtgggcg tttggtcact actatatttt gccagctcct gtcgaagagc atggatatgc
     2221 acattcaagg tattggtgtc atccacgtag tcttctttcc agatcttttc atagaggtct
     2281 gtcttggaga aaactcgctc tggattgcta gctaaaatcc acagcaattc aaatgattta
     2341 acagttaaat ccagcatttc ttctcctatt tgaacttcat gactactatg attcattttt
     2401 agattgccaa gggaaatcag ctctgtttcg cccccacgat gaaggcggcg caaaatattg
     2461 tggacacgca aaaccagctc acgtgggcta aaaggcttag caataaaatc atctgctccc
     2521 aagctcaggc cgtaaatctt gtcctgttca ctggtcttag cagtaataaa taggaaaggc
     2581 tgctctggtg ataagtattg aacctcactg attaaatcat aaccatccat ccgaggcatc
     2641 atgacatctg tgataatcaa atcaatcggt tttttcttga acagctctag cgcttccagt
     2701 ccatcatggg ctaccaagac ctgataacct gcctgaatta agtatctctg atgaatatct
     2761 gtgatttcta cctcgtcgtc aacgagtaaa attgtctttc ccatctgtct ctcctttgat
     2821 aaaaacagtg ctatactgct ttaagtataa cactattttt attgttttac gatgatttta
     2881 atctgctatt gaaagaaagt cctgattgct gattgagttg ttaaactagt cttagtcagc
     2941 ccactatcca tcatttattt ccttagctga ttagttttct tcttttttgt ttttcaaacc
     3001 tagaccaccg agtaaacctg caagcgcaag cccaaggaaa ccaatacttg ccattgatgt
     3061 ttgagtctca ccagtatttg gtagcatagc tttatcctct gacatcatcg tatcagacat
     3121 cttgttagca gaagcagcca tgttttcacc tgccatcgtg ttggtagaac ttgtcatggt
     3181 gtcagcaggc atgctatctg tagtacctgt agcatgattg tgattcatcg gagtcacgcc
     3241 agaaccagag ttagaaggtg ataatgaacc atttgctgtg tctgatgttt ctttaacatt
     3301 tatcttaata gtgacttttt tagttgctac gatgttgtcc aa
//
LOCUS       DAVKJJ010000093         3138 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000093 DAVKJJ010000000
VERSION     DAVKJJ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.093"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            280..1566
                     /locus_tag="VW144_001978"
     CDS             280..1566
                     /locus_tag="VW144_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9601371.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAVQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINFYKEDGTSVTVRA"
     gene            1581..1892
                     /locus_tag="VW144_001979"
     CDS             1581..1892
                     /locus_tag="VW144_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601372.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCLFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(1821..1970)
                     /locus_tag="VW144_001980"
     CDS             complement(1821..1970)
                     /locus_tag="VW144_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601373.1"
                     /translation="MRFIVGRFTSFSLGIEFSPTSKLDDLLFKIAFLMILATWIKARK
                     TKEAT"
     gene            2152..2880
                     /locus_tag="VW144_001981"
                     /pseudo
     CDS             2152..2880
                     /locus_tag="VW144_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
BASE COUNT          970 a          543 c          679 g          946 t
ORIGIN      
        1 atccttttcc attataggag caacagagtg ggagtagtca tctaaggact aatttatgta
       61 tttttacgag tcagtatctt gggatactgg tttttacttt tctagacttt ttgactactt
      121 gttaaaactg ggataatttt cgactgttta acagttatta tgcaaagtct aaaagattag
      181 aattgtcaaa acaatccgtc taggcttgat tttatccttt atttactata aaatgagaag
      241 gaaaaatgtc aaacttttat attgcaaata ggagaaatca tgacaaaaac attaaaacgt
      301 cctgaggttt tatcacctgc agggacttta gagaagctaa aggtagctgt tcagtatgga
      361 gcagatgctg tttttatcgg tggtcaggcc tatggtcttc gtagccgtgc gggaaacttt
      421 actttcgaac agatggaaga aggcgtgcag tttgcggcca agtatggtgc caaggtctat
      481 gtagcggcta atatggttat gcacgaagga aatgaagctg gtgctggtga gtggttccgt
      541 aaactgcgtg atatcgggat tgcagcagtt atcgtatctg acccagcctt gattatgatt
      601 gcagcgactg aagcaccagg ccttgaaatc cacctttcta cccaagccag tgccactaac
      661 tatgaaaccc ttgagttctg gaaagagcta ggcttgactc gtgtcgtttt agcgcgtgag
      721 gtttcaatgg aagaattagc tgagatccgc aaacgtacag atgttgaaat tgaagccttt
      781 gtccatggag ctatgtgtat ttcatactct ggacgttgta ctctttcaaa ccacatgagt
      841 atgcgtgatg ccaaccgtgg tggatgttct cagtcatgcc gttggaaata cgacctttac
      901 gatatgccat ttgggaaaga acgtaagagt ttgcagggtg agattccaga agaattttca
      961 atgtcagccg ttgacatgtc tatgattgac cacattccag atatgattga aaatggtgtg
     1021 gacagtctaa aaatcgaagg acgtatgaag tctattcact acgtatcaac agtaaccaac
     1081 tgctacaagg cggctgtgga tgcctatctt gaaagtcctg aaaagtttga agctatcaaa
     1141 caagacttgg tggacgagat gtggaaggtt gcccaacgtg aattggcaac aggtttctac
     1201 tacggtatac catctgaaaa tgagcagttg tttggtgctc gccgtaaaat tcctgagtac
     1261 aagtttgtcg ctgaagtggt ttcttatgat gatgcggtac aaacagcaac cattcgtcaa
     1321 cgaaacgtca ttaacgaagg ggaccaagtt gagttttatg gtccaggttt ccgtcatttt
     1381 gaaacctata ttgaagattt gcatgatgcc aaaggcaata aaatcgaccg cgctccaaat
     1441 ccaatggaac tattgactat taaagtccca caacccgttc aatcaggaga catggttcgt
     1501 gcattaaaag aaggactcat caatttttat aaggaagatg gaaccagcgt cacagttcga
     1561 gcttaagaaa ggaaaaggaa atgatagagg cacagggttt cttagtggat aagcaaacaa
     1621 gatgcattca ttaccatagc aagctggata ttattgcttt acaatgctat gattgtaaaa
     1681 agtattatgc ttgttatcgg tgtcatgatt cattagaaaa tcaccctttt gagccttatc
     1741 ccttatcttt gatacaggat aagcctattt tatgtggtgt ttgtctaaaa ctattaacat
     1801 ataagcaata taaagaaagc ttaagttgcc tcttttgttt ttctcgcttt aatccaggtt
     1861 gccaaaatca taaggaacgc tattttaaat agcaaatcat ctagttttga agtaggagaa
     1921 aactcaattc caagagaaaa tgaagtaaat cttcccacaa taaaacgcat aatatcaaga
     1981 ttgttcaata cctgatacta tgcgttttta agattttaaa gacttttttc ctagcccctt
     2041 tttactttat ctggtatttt gactacttgt taaaactggg gtaattttcg actgtttaac
     2101 agttattatg caaagtctaa aaggttagaa ttgtcaaaac aatccgtcta gagtatgcgt
     2161 gatgccaacc gtggtggatg ttctcagtca tgccgttgga agtacgacct ttacgatatg
     2221 ccatttggga aagaacgtaa gagtttgcag ggtgagattc cagaagaatt ttcaatgtca
     2281 gctgttgata tgtctatgat tgaccatatc tcagatatga ttgaaaatgg tgtggacagt
     2341 ctaaaaatcg aaggacgtat ggagtctatt cactatgtat taacagtaac caactgctac
     2401 aaggcggctg tggatgccta tcttgaaagt cctgaaaagt ttgaagctat caaacaagac
     2461 ttggtggacg agatgtggaa ggttgcccaa cgtgaactgg ctacaggatt ttactatggt
     2521 acaccatctg aaaatgagca gttgtttggt gctcgtcgta aaatccctga gtacaagttt
     2581 gtcgctgaag tggtttctta tgatgatgcg gcacaaacag caactattcg tcaacgaaac
     2641 gtcattaacg aaggggacca agttgagttt tatggtccag gtttccgtca ttttgaaacc
     2701 tatattgaag atttgcatga tgctaaaggc aataaaatcg accgcgctcc aaatccaatg
     2761 gaactattga ctattaaagt cccacaacct gttcaatcag gagacatggt tcgagctctt
     2821 aaagaggggc ttatcaatct ttataaggaa gatggaacca gcgtcacagt tcgtgcttaa
     2881 tgtagttgtt tagttttaaa aaattatgca aagctccata tacaacactt aaacgagatt
     2941 aaagaatggc gaaatcccct gatgcgcaag agattagctg tcttttttat tttttaagtg
     3001 ataaagtcgg agcttaggta tcaaagccta tcaaattaaa caaagaagcg atgtcttaga
     3061 tattttgaaa aaaattaata agcagaaaac tctctattat tttgttgtat agagtttttt
     3121 gttaataaaa tttcacaa
//
LOCUS       DAVKJJ010000094         3075 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000094 DAVKJJ010000000
VERSION     DAVKJJ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.094"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(<1..347)
                     /locus_tag="VW144_001982"
     CDS             complement(<1..347)
                     /locus_tag="VW144_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601374.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFLNYLTNRKYSLNMNRNGCMGDFPHDFF
                     DIYLDHVAKYAYE"
     gene            complement(405..1118)
                     /locus_tag="VW144_001983"
     CDS             complement(405..1118)
                     /locus_tag="VW144_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEU9601375.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIGRDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1577..1957)
                     /locus_tag="VW144_001984"
     CDS             complement(1577..1957)
                     /locus_tag="VW144_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEU9601376.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNA
                     AALLQAGAELSVIQIQLAELEINK"
     gene            complement(2379..>3075)
                     /gene="phtB"
                     /locus_tag="VW144_001985"
     CDS             complement(2379..>3075)
                     /gene="phtB"
                     /locus_tag="VW144_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEU9601377.1"
                     /translation="HQDSGNTEAKGAEAIYNRVKAAKKVPLDRMPYNLQYTVEVKNGS
                     LIIPHYDHYHNIKFEWFDEGLYEAPKGYTLEDLLATVKYYVEHPNERPHSDNGFGNAS
                     DHVQRNKNGQADTNQTEKPSEEKPQTEKPEEETPREEKPQSEKPESPKPTEEPEESPE
                     ESEEPQVETEKVEEKLREAEDLLGKIQDPIIKSNAKETLTGLKNNLLFGTQDNNTIMA
                     EAEKLLALLKESK"
BASE COUNT          925 a          607 c          503 g         1040 t
ORIGIN      
        1 tgttcgtagg catattttgc tacatgatct aggtatatat caaaaaagtc atgaggaaaa
       61 tctcccatac agccattacg attcatattg aggctatatt ttcgattagt aagataattc
      121 agaaatatat ttcctattac tctaagatta tctacttgtg aatcccttct cccaatataa
      181 tccccttctt tgactaatga tggagatttc caagtaaaaa ttaaatcgcc agagaaattt
      241 ttcttatcaa aaaacttaaa aagagattga taaatactat ttgctaattg acaagtgtca
      301 gatgtaaatt cataattaac agcttcatta aaatcaactt cagacatttc aatgtactct
      361 tcaatagtaa tattagcttt tttgtagtca ttagctcgtc tcctttaaaa tgtgatcttt
      421 tcaagaatat ttttatctaa acaatccagc aagtcttggt aagaatagac ttcgtaagtc
      481 ggctgggctt gtgtgtgatt ttcgaggtga tgaggattat accagatagt gtcaatcccc
      541 gcattattgc caccttgaat gtcggcggtt agagaatctc caatcatcag cgtcttttct
      601 ttactaaatc cagcaatttg ctggccaatc ttttcataaa aaagagcatc cggcttttga
      661 gtttgcaact gttctgagat aaagacttga ttgaaataag gtgctagacc agattgagcc
      721 aaacgtcctg tctgaatggc agtaatgcca tttgtcgcag catacaagtt ataatcacgc
      781 ccaatgaggc tgtccaagag atcatgagcg cccgatagtg tttgtccctg ctgggcgagg
      841 taaaattggt aacgctgggc aagaaaacta ccgtcttttt cttgtccaaa atgagcaaat
      901 aaacgagaaa agcgcgtgtt aaccagctct tgtttactga ttttcttcag ctccaagtct
      961 ttccagagag ccttgttcat aggaacgtaa taatctttat aagcctgaat atccgcaact
     1021 ccttcttctt ttagaagttg ggtcaaagcc acatcctcag cagcatcaaa atcaagaaga
     1081 gtgtggtcga ggtcgaagag tagaaatttg tagaacaatt tgaggttttc ctttctgaaa
     1141 attcattaag aacattatat cataaagcac ctcatacaat taactaattt aatcacttaa
     1201 aaaaaattcg aacactttct atacaactga cagctcaaat ctttcagaat agaacaatac
     1261 taactatcga acaccctgtc ttcataaata catatgtaat tctaggccta gaattcctat
     1321 aaactaaatg ctttcatact cttccaagta attgattgcc ttaaatttta atttttgaag
     1381 gtttctaaag ctagaatagc cccatcacaa tcagttttga ttgattcaca atttagaaac
     1441 actatagttt cactcctgtt aaaataaaaa ggaactgcat aaagcaatcc ctttctgatt
     1501 ttgaaatcat ttacttaaca ttttatagtt gagataatca atagcttatc tataaaaaga
     1561 gttatagtaa aattccttat ttattgattt caagctccgc taactgtatt tgaataactg
     1621 acagttctgc accagcctga agaagagcag ctgcattata ggcaccttct acaattggaa
     1681 ccctgttgat gatgatactt ttatcactga aatcagtcac catttttaag ttcattttag
     1741 cagaacctag gtcaaaaaag gcaagtaaag tatctgctgg attttcggaa acaaccctat
     1801 ctacttgatc aaaactcgtt ccaattcctc cgccctcggt tcctcctaca taagtaatcg
     1861 gaacatcttt agctacttta ctaatcagtt caacaacacc ttctgcaatg tgtttggaat
     1921 gtgaaacgat aacaagacca ataccaatac tttccatcaa accactccaa tttctaaaat
     1981 agcagtaaag agtaatcctg atgagaatga tccaggatca atatgtccaa gaaaccacat
     2041 gctcctaaga caagagctaa cagactggcc atcaataata gtattgttct ttttttcatc
     2101 attactcctt aactagtgtt taactgatta attagccagt aaatagttta tctttattta
     2161 cactatctgt taagatatag taaaatgaaa taagaacagg acagtcaaat cgatttctaa
     2221 caatgtttta gaagtagagg tatactattc tagtttcaat ctactatatt ttgcacattt
     2281 tcatgaaaaa aatgagaact agaactcaca ttctgctctc atttttcgtt ttcccgttct
     2341 cctatcctgt ttttaggagt tagaaaatgc tgctaccttt acttactctc ctttaataaa
     2401 gccaatagtt tttcagcttc tgccataata gtattgttgt cctgggtgcc aaatagtaaa
     2461 ttatttttta atcctgtgag agtctctttg gcattggact tgataattgg atcctggatt
     2521 tttccaagta aatcttcagc ctctctcagt ttttcttcaa ccttttcagt ctcgacctga
     2581 ggttcttctg attcctctgg tgattcttct ggttcctctg ttggttttgg agactctggt
     2641 ttctcgcttt gcggtttctc ttctcgaggg gtttcttcct caggtttttc tgtctgaggt
     2701 ttctcctcgc ttggtttttc cgtttgattg gtatcagctt gaccattttt gtttctttga
     2761 acatggtcgc tagcgttacc aaaaccatta tctgaatgcg gacgttcgtt tggatgttcg
     2821 acatagtact tgacagtcgc caaaagatcc tcaagagtat accccttagg tgcctcataa
     2881 agaccttcgt caaaccactc aaatttgatg ttatggtaat ggtcataatg aggtatgatt
     2941 aaactaccgt ttttgacttc tacagtatat tgaagattgt aaggcatacg atcaagtggc
     3001 accttcttag ctgctttcac gcggttgtag atagcttctg ctccttttgc ctcagtattt
     3061 cctgaatcct gatgg
//
LOCUS       DAVKJJ010000095         3055 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000095 DAVKJJ010000000
VERSION     DAVKJJ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.095"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(154..624)
                     /locus_tag="VW144_001986"
     CDS             complement(154..624)
                     /locus_tag="VW144_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9601378.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(641..2914)
                     /locus_tag="VW144_001987"
     CDS             complement(641..2914)
                     /locus_tag="VW144_001987"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEU9601379.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
BASE COUNT          892 a          548 c          686 g          929 t
ORIGIN      
        1 tacggcaagg cgacgttgac gcggattgaa gagattttcg aagagtataa atcttcctag
       61 gatagagcaa aacgcatagt atcaagggtt ttctgatgtt aaagactttc taccaggttt
      121 tttaaaagca taattgttag ttgtagtcat ttattattct tcaaagaaaa atggtggggc
      181 gaattttttc agttcttcaa agcacttttg agcagtatct gcatcttcac agatgataag
      241 acagacatca ttaccacaaa gggtagcgat agcgtcaggg aagctcaaag tatcaatgat
      301 agaaccaaag gattgagcca gtccaggaag ggtttttagt aggacttggt gttgaactgg
      361 gcgcatccag acaagggcgt cttccatgta gagttcgaga cgtttttccc attttgagat
      421 ggaaccattg ttaagaacat aataagcgct atcttcttcg cggacttttg ataggttcat
      481 atttttgatg tcgcgtgaga gggttgcctg ggttacttga atgtcgttct cagcaagaag
      541 ggcttgcaac tcagcctgtg tatgaatctt gttttttgtg ataagagcgc gtataagttg
      601 gtggcggtgt tctgatttat tcataataat gtaactcctt ttagcaaggt aaggtaagca
      661 tggactgagc gaggtcgaca gtcaagtggt agtctgtatt gtcacggatg gtgatttcaa
      721 agtcagtagt atagaggact aaacggagag tgtctccttc ttttagcttg taaatagttg
      781 gctgcagttc aaattgaacg tccatccatt catctgcagt aatatcctct actaacagta
      841 aatcatttct attttgtaaa ttaaggtaac cttttgtcac gactcgttgt gcctctggtc
      901 taaatggcaa ttcacagaga ttttccaaca tgtgatagcg accgttatca atggttctag
      961 cacttaaaat agctggataa ggttgtaggt atttcttttg cccaaattct agcagttggg
     1021 cagataagag ccccttgttt gtactggatt tgatacgaag attgagctga gcgcgaccgt
     1081 ttaggtggag atctttagtc acaggaaggt taatagtaat ctgattggct ttcccttgat
     1141 agagctctgt attgaaggtt tggtatgtct taccatagcg ctcaaaatcc ttatctgggt
     1201 actggttttg aatagcttgc tcttcttgac caagtgagaa ggtttcacag ttttcttgcc
     1261 caccgaagtt atcaagtgat aaccaagtct gtggagctgt attgtcctgc cagataacag
     1321 taggaagttg aaagtctgtt tcctgtccta gtaatttctt ggtcaataag gcatttatgg
     1381 actcacggaa gtcaattgat tgccaattgt tcatgtaaac atgagcacca ttatggaaaa
     1441 agagatgctt gtgtatatga gtaggaagag catggaacat ctggtaaaca tgaagtggtt
     1501 tgacattcca atcctgagaa ccatgagtaa agacaacctc tgcctttact ttatgggcat
     1561 tgagcagata attgcggtca tgccaaaact gattgtagtc cccagttttt cggtctagct
     1621 gagctttcac tttttctaag tcagcttggt gagcttcatt gccacggata tagtcgccag
     1681 ctaagagatt acgagaatag gttaactcag caagggagtc aaagtcctca cctggataac
     1741 cacctgggct agtcaccaga ccgttttcac ggtagtagtt gtaccatgat gaaattcctg
     1801 cctcggcaat gataacttct aaaccatcga ctcctgtagt cgcaagacca ttggacatgg
     1861 tacctagata ggaaagtcct gttgtagcaa cttttccgtt tgaccaatca gccttgactt
     1921 gacgctggcg cgtgtgatca gtaaaggcac ggcaacggcc gttaagccaa tcgatgacat
     1981 ttttataagc ctcgatttgc tggtagtctc cattagtcat gaaacctgtc gagtctttgg
     2041 taccaacacc tgagacatag agattggcaa agcctctcgg aaggaagtag tcgtttagtg
     2101 tatagctaga gttgatgtga gttagctttt cctcagcctc tgctataagc tcagctttac
     2161 cttggggttg gacgagattt agttgaggtt tctctagctc aatcttgtga ggaagcttaa
     2221 cctcaagctc gccctccatc ttgtagagag ccttgtcact agccttgtca ttggttccct
     2281 gatgataagg gctggctgtc atgatggcag ggatttttcc atcaaaacga gggcgaataa
     2341 tgctaacctt tactaggtct gatagccctt tttggtcagt atcgacacga gactcaacgt
     2401 aaacgacttc acgaatgaca tcctggttag aaaaagtagc caaactcttg ccgttaaagt
     2461 agtggtagtc attatcctcc ggaataagac catcactaac aagttggtcg ataagagtat
     2521 ttcctttttt ggtgcgagta ttgagtaact gatagagatt ttcaatcaag tcaccatata
     2581 taatgggaaa tccagtttct ttacgaaaaa cgtcactatc ttcgaagtca accaaataag
     2641 aaaagcctaa aagttgaaaa gcaacagtat aaaaaatatc tgctgtcagt tcatcttctg
     2701 attgaaaaaa tgtcagcagg tctgtttttt tatcagctgc taggatagaa agtgggtagt
     2761 tggtgtcttg ataagtgaaa aagaaacgac gtaaaaaggt ttcaagtgag tctttgtgat
     2821 tggctgtatt ttgtaaatca aagccacatt tttttagttc agataagaca ttttcttttg
     2881 gaaaattgat ataactatat tgattaaaac gcatagaacc tccatataga atgacagtta
     2941 aggttattat atcaaaaaaa aagcagaaag ggaattgtta acttcaaaag gaaataatcc
     3001 aataaaaatg aataaagtac taaattcaat atagagaaca gagtaacaat aagaa
//
LOCUS       DAVKJJ010000096         2938 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000096 DAVKJJ010000000
VERSION     DAVKJJ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.096"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(120..653)
                     /locus_tag="VW144_001988"
     CDS             complement(120..653)
                     /locus_tag="VW144_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU9601380.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(742..1518)
                     /gene="recX"
                     /locus_tag="VW144_001989"
     CDS             complement(742..1518)
                     /gene="recX"
                     /locus_tag="VW144_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEU9601381.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDINENIVSQVIANLKEEKW
                     INDSQYAYAIINANQLSGDKGPYVLTQKLAQKGISKSTIEEILNDFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYFDAKIAFDELDSQVDEETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            1556..2920
                     /gene="rlmD"
                     /locus_tag="VW144_001990"
     CDS             1556..2920
                     /gene="rlmD"
                     /locus_tag="VW144_001990"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9601382.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     SLKK"
BASE COUNT          929 a          533 c          535 g          941 t
ORIGIN      
        1 atctttttct agtaatgtgt tgtaactctg ctataataga tttattcctt tttgtgttta
       61 cacaatttat tttatactac caaaaaaggt caggattttg ttcctgacct ttgacaactt
      121 taccgattct ttagttctac atagcgcttg taccaaatgt ttacataggc ttctgagaaa
      181 ggaccacgtc cattgttaat ccaatcaaca agaattttga catgttcttt taaaatatag
      241 tccaagtcat cagaataatt cattttgcgt ttgtgacgct cgtactcttc aacgtccaag
      301 agacgttttt ccccatctgt aaaaatttta acatccaaat cgtaatcaat atacttcagt
      361 gcttcttcat ccagatagta ggggctagcc atattgcaat agtaagaagt tccattatca
      421 cgaatcatgg caatgatatt aaaccaatat ttcttgtgaa agtaaacaat agccggttct
      481 cgagtgaccc aacgacgacc atcactttcg gtaacaagtg tatgatcgtt gacaccaata
      541 atggcgtttt ctgttgtttt tagtaccatg gtgtcccgcc aagttcggtg gagactccca
      601 tcatgcttat aactttgaat tgtaataaag tcgccttctt ttggaagctt cataactaac
      661 caactttcta caatttataa gtttatcatt tactattgta ccataaaatt acccaaaatc
      721 tgtgaatttc acttggaaat attaaagata ttctctaaga gcgcttgcta tatccgaaaa
      781 atcgtagccc tttcgtgcta aaacttgagt taaacgctgc ttcagttcgt atccttcata
      841 ctttcgggca tacttagtat attgcttatc aagttccttg aagatgagtt cctgagtcgt
      901 ttcttcatca acttgactat ccaattcgtc aaaggcaatt ttagcatcaa aataagagaa
      961 gcccttgtta gtcaagttct ggataatctt atcttgcagg gcacgagctg gaagttttcc
     1021 ctcatatttt ttcaatagtt tattggctac acgttgagca acttccgaaa aatcaaaatc
     1081 attcaagatt tcttctatag tagattttga aattcctttt tgtgctaatt tctgagtcag
     1141 tacataaggt cccttgtctc ctgaaagttg attggcattg atgatagcat aagcgtactg
     1201 gctatcatta atccacttct cttctttaag attagcaatg acttgagaaa cgatgttttc
     1261 attaatatca tattttttca gatattctct gacctctttt tcagtacgtg ctttaaagga
     1321 taagtggtag agggccagat tcttaccata agaaaattga gcaaagtctt gaatctcttt
     1381 caattcctct tcgcttatca ccttatctct cgataacata aaacgaacaa ttgtgtcttc
     1441 ggtgatatag catttgtcgc cattatcaag ctccatcaga tagagtcttt ttttcttttc
     1501 aagttttgtg attttcatag ctctattata actcaaaatg tgataagata ggggtatgaa
     1561 tctgaaagtg aaacaaaaaa taccattaaa aatcaagcgc atgggaatta acggtgaggg
     1621 aatcggcttt taccaaaaaa cattagtctt tgtaccagga gctctcaaag gcgaagatat
     1681 ctattgtcag attacttcta ttagacgcaa ctttgttgaa gcaaaattac tgaaggtcaa
     1741 caagaagtct aaatttcgaa ttgtgccatc ttgtactatt tataatgaat gcggaggctg
     1801 ccaaatcatg cacctgcatt atgataagca gctggagttc aagacggact tacttcatca
     1861 agcgctgaaa aaatttgctc ctgcaggata tgaaaattat gaaattcgtc caactattgg
     1921 aatgcaggaa ccaaaatatt acagagctaa gttacaattt cagactcgaa aatttaaaaa
     1981 tcaggtcaag gcgggcttat atgcacaaaa ctctcactat ttagtagagt tgaaagactg
     2041 cctggtacaa gataaggaaa cccaagtgat tgctaatcgc ttagcagaat tacttactta
     2101 tcaccagatt ccaatcacgg atgagagaaa agttctaggt gtccgtacta ttatggtccg
     2161 acgcgcgaga aagaccggac aggttcagat tattattgtt acaaaccgcc agcttaattt
     2221 aactcaattg gtaaaagagt tggttaaaga tttcccagaa gttgtgacag tagctgttaa
     2281 tacaaataca gctaaaacca gtgagatata tggtgaaaag acagagatta tctgggggca
     2341 agagagtatt caagaaggtg tactcaatta tgaattttca ctatcccctc gagcttttta
     2401 tcaactaaat cctgagcaaa cagaagtcct ctatagcgaa gcagtaaaag cgctggatgt
     2461 tgataaagaa gaccatttga ttgacgctta ttgtggagtt ggaacgattg gatttgcctt
     2521 tgcaaagaaa gtaaaaacac tcagaggtat ggatattatt ccagaagcta ttgaagatgc
     2581 caagcgaaat gctaaaagaa tgggatttga caatactcat tatgaagctg gaacggcaga
     2641 agagattatt cctcgttggt acaaggaagg ctaccgagca gatgctttga ttgttgaccc
     2701 accacgtaca ggtctggatg ataagttatt agatactatt cttacttatg taccagaaaa
     2761 aatggtttat atttcttgta atgtttcgac cttggctcgt gatttggtac gcttagtaga
     2821 agtctatgat cttcattata tccagtcggt cgatatgttc ccacatacag ctcgaactga
     2881 agctgttgta aaattaataa aaaaaagttt aaaaaagtag ttgacaaagt ttgaaaag
//
LOCUS       DAVKJJ010000097         2924 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000097 DAVKJJ010000000
VERSION     DAVKJJ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.097"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(659..1870)
                     /locus_tag="VW144_001991"
                     /pseudo
     CDS             complement(659..1870)
                     /locus_tag="VW144_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880290.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(1872..2870)
                     /locus_tag="VW144_001992"
     CDS             complement(1872..2870)
                     /locus_tag="VW144_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HEU9601383.1"
                     /translation="MDIVGFLNDHFVEKNTHSVSNLLFKDKYFMRSFLVGVVNQPYFR
                     LLESENDLRVFWNNCKSSAAILKPRNEAGSNGIKKVYPNEKIDSVYFEKNYIIEEYID
                     IDNMITCDGYSIGSEIKRFYVHEYEELLFNTLSSSGYYLVRTSSLYNDSEKIEYIEKA
                     LEECRKVLNVFSINSEITPFHFEWFLELSTGKIVFCEVGKRFGGGDIPQLIEDSHGVD
                     VIKEYWDILTNSQGLKKINFSKVISRPANISATFALYRKQGKVIEVPDEKELAWAKKV
                     YIFIKPNTYYKVSENVVENSMLVQFNCKNSCEFEKRLDELKHISQKFKYVITDSGE"
BASE COUNT         1072 a          507 c          370 g          975 t
ORIGIN      
        1 tataatatat aaaactaaga aatcattcaa agtgtccaaa ctggacattt tgaactaaaa
       61 ttaatttatt aaacatatct cataagcata tctattatag gtaagacaat attaagaaag
      121 acggtcaaaa tctattaacc gtctaaagat gttataagct attaaaaaat cagcagtaat
      181 ccggtaacaa aagtcacata attttgttaa ggaagggatt aatgatgtcc agattactac
      241 taaccagcaa attgaaaaat ggtcaacatg atatttctaa tgtgaatctc ttactttcag
      301 aacctaaaag ttgaataata gccaccccct acttatcaat aaaattttcc gttgcattaa
      361 cagatgtata tagaccactc catggatttc aaagcaatta actagacttt atttgtcaat
      421 aaccacaaaa ttagattaga aaaatcatta aaaattaatt ggagtccttt gtgatgcttt
      481 ttcaataaaa gtaatacttc tcaatatttt aactgaacta aagaaatagc ataatggtat
      541 cttgattgta ttcttctaaa aaggattgcc cttcacattc aactgtgatt gattaaattc
      601 caaaaatata ttccaaaggc gctctaaatt ctctatatca gctatttttt atcatctatc
      661 aacttctatc atacctcttc aatagcgtgc taaatattag cgataatatc gtaatagata
      721 caccaataat aaaaaaatta aaagtattgt taggatttaa aactactgag aaaaaaacag
      781 tacctagtgg cataaaaaga atagctaccg taaagatgat tccaaatact ctgccaagaa
      841 actcattatc tacgtctctt tgaacaatag agaaaaattg gatattaaaa atggaaagaa
      901 atagactaaa taatgctgga gtaaaggcta aaataatgac gttgcggaac atataataga
      961 tcggcgctgt tagcattaac atcaaacctg aatatgctaa aaagagcatt aaacgtttgc
     1021 ttgacaagga tttattgata aatccactta atatcgcacc aataaagcca ccaatcgctt
     1081 cggctgttaa aaaggttcca tatagcccac ttgaaatact tccaaacatt tgattactgt
     1141 atggcagcaa caaattatag gctgctagga aaaagttaac aagtgctgaa agggcaatta
     1201 tagtgagaat ttgtttatga ctatatacat acgtaaatcc tatttttaaa tcatcaaaga
     1261 ttcctcttat cgtcatattc tcttttgtat ccacctcctc atttacagga gtaatgaaga
     1321 aaattagtga ggcagcaatt aaaaacgata atccatctag taataacaca ccatgtattc
     1381 caagtaactt atataagaaa attgcaatca taggaattgt tactttaatc acagtacttg
     1441 ttgtttctag caatgaatta agttatgata tactgtcctt ttttacaatt tctttcgtaa
     1501 aagctttgta tgatggccct gaaaaagcac tcataaaagc caaaatgaca tttgttatga
     1561 ctatcgcata aaccaaccat ttttcttggg atataaatga aagagttatg caagcaatac
     1621 cacataagat atttgtagta ataataattt ttttccgctt gaaactatct gcaataactc
     1681 caccaaataa attaaataga acacctatca tactttctaa tgattgataa attgcaacca
     1741 ctgatagaga tgttggatta agaccagcaa gaaaggtatt attggcgaaa tcaaacatga
     1801 tatctccgat tcgagatatc atcctactag ctacaatgga tagaatattg tgatgttctt
     1861 ctctaatcat tttattctcc cgaatcagta atcacatatt taaacttttg tgatatgtgc
     1921 ttaagttcat ctaacctttt ctcaaattca caactatttt tgcaattaaa ttggaccaac
     1981 attgaatttt cgactacatt ttcagatact ttatagtaag tatttggttt aataaatatg
     2041 tacacttttt ttgcccaagc caattctttt tcatcaggta cctcgataac cttaccttgc
     2101 tttctatata aagcaaacgt tgctgagata ttagcaggtc tagaaatcac tttagaaaaa
     2161 tttatttttt tcaatccttg tgaattagtc aaaatatccc aatattcttt aattacatca
     2221 actccatgac tatcctcaat taactgggga atatctcccc ctccaaatct ttttccaact
     2281 tcacaaaaga ctattttacc agttgataat tcaaggaacc attcaaaatg aaaaggggtt
     2341 atttcagaat tgatagaaaa aacattcaaa actttgcgac attcttccaa tgctttttct
     2401 atgtattcaa ttttttcact atcattatat aacgaactag tccttacaag ataatatcca
     2461 ctagaactta aggtattgaa caatagttcc tcatattcgt gaacataaaa tcgcttaatc
     2521 tcacttccaa ttgaataacc atcacatgtt atcatgttat cgatatcaat gtattcctct
     2581 ataatatagt ttttttcaaa atacactgaa tcaattttct cgttagggta aacttttttt
     2641 attccattac ttcctgcttc atttctaggt tttaaaattg cagcagaaga tttacaatta
     2701 ttccaaaaaa cacgtaaatc attttctgat tctaaaagcc taaaatacgg ttgatttact
     2761 actccaacta aaaatgaacg catgaagtat ttgtctttaa ataataaatt agacacagag
     2821 tgagtatttt tttctacaaa atgatcattc aaaaaaccta ctatatccat gtcttcctca
     2881 ctcaaggttg taatactctc aacttctgta ttttgactta tcct
//
LOCUS       DAVKJJ010000098         2901 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000098 DAVKJJ010000000
VERSION     DAVKJJ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.098"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            <1..979
                     /locus_tag="VW144_001993"
     CDS             <1..979
                     /locus_tag="VW144_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9601384.1"
                     /translation="FSFISLAVVIFVGVNLIINKEINSLYLIGYLLAAMKIKDSLDAS
                     KEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKDVEFAYNKDAKVLNGVS
                     FKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGKDIKEISTESLFDKVSI
                     VFQDVVLFNQSIMENIRIGKQDASDEEVKRAAKLANCTDFIEKMDKGFDTVIGENGAE
                     LSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQESLNNLVKDKTVVIISHR
                     MKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTKMAEEFIY"
     gene            990..1574
                     /locus_tag="VW144_001994"
     CDS             990..1574
                     /locus_tag="VW144_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU9601385.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            1574..2269
                     /locus_tag="VW144_001995"
     CDS             1574..2269
                     /locus_tag="VW144_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9601386.1"
                     /translation="MPTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKMAMRVRGISF
                     KNPVKYLEYVSMPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VAGLIVGGLIWLK"
     gene            2257..>2901
                     /locus_tag="VW144_001996"
     CDS             2257..>2901
                     /locus_tag="VW144_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9601387.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLKIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKG"
BASE COUNT         1037 a          374 c          555 g          935 t
ORIGIN      
        1 atttagcttt atatctcttg ctgttgtgat atttgtcggc gtaaatctaa ttattaataa
       61 agagataaat tctctctacc ttataggata tttactagct gctatgaaga taaaagactc
      121 tttagatgca tctaaagagg gcttgatgga aatattttat ttatcgccca aaatagaaag
      181 attaaaagaa attcaaaatc aagatttaca agaaggcgat gactatagct taaaaaaatt
      241 tgatattgat ctaaaagatg ttgagtttgc ctacaataaa gacgcaaaag ttttaaatgg
      301 tgtaagtttt aaagctaagc agggagaggt cactgctttg gtaggtgcaa gtggctgcgg
      361 taaaacaact atcttgaaac ttatatcaag actttatgat tatgacaagg gacaaatctt
      421 aatcgatggc aaagatataa aagaaatatc aacagaatct ctttttgata aggtctctat
      481 agttttccaa gatgtggttc tctttaatca aagcattatg gaaaatatta gaatcggaaa
      541 gcaagatgca agtgacgaag aggttaaaag agcagcaaaa cttgcaaatt gcacagattt
      601 tatagaaaaa atggataaag gtttcgatac agttattggt gagaacggag ctgagctatc
      661 aggaggagaa agacaaagat tatcaatagc cagagccttc ttaaaagatg cgccgatatt
      721 gatcttagat gagataacag caagccttga tgttaacaac gagaaaaaga ttcaagagtc
      781 tttaaataat ttagttaaag ataaaactgt tgtaatcatt tcacatagaa tgaaatccat
      841 agaaaatgca gacaagatag tagttcttca aaacggaaga gtagaaagcg aaggtaagca
      901 tgaagagctt ttacaaaaat caaaaattta caaaaattta atagaaaaga caaaaatggc
      961 agaagaattt atttattagg aggactacaa tggataataa aaaattaaaa gtaaaagatt
     1021 tagtaagtat cggtgttttt ggcgtaattt attttgcctt catgtttgga gttggtatga
     1081 tgggcttgat tccaatattg ttcttaatat acccgacagt attagccata gttgcaggaa
     1141 ctgttgttat gttatttatg gctaaggttc aaaagccatg ggcactattt atatttggta
     1201 tgatatcacc acttgtgatg tttgcagctg gtcataccta cgtagttgtg gttttatcac
     1261 ttatagtaat gataatagca gaattaatta gaaagattgg taattataat tcatttaaat
     1321 acaatatgct ttcttatgca atcttcagca catggatatg tagctcttta atgcaaatgc
     1381 ttttagcaaa agaaaaatat atggagtggt ctttgatgac gatgggaaaa gattatgttg
     1441 atgtattaga aaagttaata acttatcctc acatggcttt agtagcctta ggtgctttct
     1501 taggaggaat tcttggagca tatataggca aggctctatt gaaaaaacac tttgaaaaag
     1561 caggcattgt ataatgccta ctttttcttc aaatgctccc tttaatccca atccgatctc
     1621 taagatatta gtcgtattta tcacaggctt aactgttatg catagcataa acatccgttt
     1681 tgagctggca attgtttgtc ttattggtat tttgctttat ttgaatggat acaaaaaaac
     1741 acttttcaaa tggattatat tgtgtgggat actttactcc ttgcctaatt ttatggtgtt
     1801 atctgaatta aaccctatag ttaagatgtt tttgagtata cctattgtta ttagaatgtt
     1861 tattttacca tttatggcag caagctttat gataaagacc tcggatgtag gcgcaataat
     1921 ttcatcgatg gataagctta agatttcaaa gaatgtatcc atacctattg cggttatgtt
     1981 tagattcttc ccatctttta aggaggagaa gaaaaacatc aaaatggcta tgagagtaag
     2041 agggataagt tttaaaaacc cagtcaaata tcttgaatat gtttctatgc cactactcat
     2101 tatatcatct aatatatcag atgacattgc aaaagcggca gaaacaaagg caatagaaaa
     2161 tccaattgcc aagaccagat acattcgcgt aaagatacag ctaattgatt ttgtttatgt
     2221 tttagcggtt gctggactta ttgtgggagg cttaatatgg ttgaaataaa aaatttaagt
     2281 cttgattatg gtgaagagca tatattagat gatatatcac tatccatagc cgagggagag
     2341 tgcgtgctat ttacaggaaa aagtggaaat ggtaagtcat ctttaataaa ttcaatcaat
     2401 ggactagctg taaggtatga taacgcaaag acaaagggcg aaataattat tgatggtaag
     2461 aatataaaaa atttggaact ttatcaaatc tcaatgcttg tttcaactgt ttttcaaaat
     2521 cctaagacat atttttttaa tgtcaatacg acattagaat tattatttta tttggaaaat
     2581 atcggtcttg caagagaaga gatggacagg cgtttgaagg atatgcttaa gatattcccg
     2641 ataaaaaatc ttttgaacag aaatatattt aatctatccg gcggtgaaaa acaaattctt
     2701 tgcattgcag cttcttatat agcaggtaca aagattatag ttatggatga gccttcatcg
     2761 aatttagata ttaaaagcat aagtgttttg gcaaagatgc taaagatatt aaaagagaaa
     2821 ggcataagca taattgttgc agagcataga atttattatt tgatggacat agttgaccgt
     2881 gtatttttaa tagataaagg a
//
LOCUS       DAVKJJ010000099         2831 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000099 DAVKJJ010000000
VERSION     DAVKJJ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.099"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            392..2551
                     /gene="pfbA"
                     /locus_tag="VW144_001997"
     CDS             392..2551
                     /gene="pfbA"
                     /locus_tag="VW144_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEU9601388.1"
                     /translation="MVKNSKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDLKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKEPVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            2535..2831
                     /locus_tag="VW144_001998"
     CDS             2535..2831
                     /locus_tag="VW144_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601389.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYNKMK"
BASE COUNT         1101 a          348 c          540 g          842 t
ORIGIN      
        1 ttcaagctaa gaaagatagc atcacttttg aatggaaggc taaagagcaa actaggaagt
       61 tggccataga tagctcaaaa ccctgctttg aggttgtaga tataataaaa tgaaataaga
      121 ataggacaaa tcgatcggga cagtcaaatc gatttctaag aatgttttag aagtagaggt
      181 gtactattat agtttcagtc tactatagaa ctgaccaagt cagtaaccta gacttaggtc
      241 aaggcggcac tgacctagtt tgaagagatt tccgaagagt ataaatttta atattttctt
      301 gtgttattcc ttgacaattc aatttggaaa atatatgata aagataatga cagcggtgtc
      361 attctatcta ttttaagaaa agtaataatc aattgttaaa aatagtaaaa aattggaggt
      421 tctgatgaaa tattttgttc ctaatgaggt attcagtatt cgtaaattaa aggtggggac
      481 ttgctcggta ctattggcaa tttcaatttt gggaagccaa ggtattttat cggatgaagt
      541 tgttactagt tcttcaccga tggctacaaa agagtcttct aatgctatta ctaatgattt
      601 agataattca ccaactgtta atcagaatcg ttctgctgaa atgattgcct ctaattcaac
      661 cactaatggt ttagataatt cgttaagtgt taatagtatc agctctaatg gtactattcg
      721 ttccaattca caattagaca acagaacagt tgaatctaca gtaacatcta ctaatgaaaa
      781 taagagttat aaggaagatg ttataagtga cagaattatc aaaaaagaat ttgaagatac
      841 tgctttaagt gtaaaagatt atggtgcggt aggtgatggg attcatgatg atcgacaagc
      901 aattcaagat gcaatagatg ctgcagctca agggctaggt ggaggaaatg tatattttcc
      961 tgaaggaact tatttagtaa aagaaattgt ttttttaaaa agtcatacac acttagaatt
     1021 gaatgagaaa gctacaattc taaatggtat aaatattaag aatcaccctt ccattgtttt
     1081 tatgacaggt ttatttacgg atgatggtgc gcaagtagaa tggggcccaa cagaagatat
     1141 tagttattct ggtggtacga ttgatatgaa cggtgctttg aatgaagaag gaactaaagc
     1201 aaaaaatcta ccacttataa attcttcagg tgcatttgct attgggaatt caaataacgt
     1261 aactataaaa aatgtaacat tcaaggatag ttatcaaggg catgctattc aaattgcagg
     1321 ttcgaaaaat gtattagttg ataattctcg ttttcttggg caagccttac ccaaaacgat
     1381 gaaggatggg caaatcataa gtaaggagag cattcagatt gaaccattaa ctagaaaagg
     1441 ttttccttat gccttgaatg atgatgggaa aaaatctgaa aatgtgacta ttcaaaattc
     1501 ctattttggc aaaagtgata aatctgggga attagtaaca gcaattggca cacactatca
     1561 aacattgtcg acacagaacc cctctaatat taaaatttta aataatcatt ttgataacat
     1621 gatgtatgca ggtgtacgtt ttacaggatt cactgatgta ttaatcaaag gaaatcgctt
     1681 tgataagaaa gttaaaggag agagtgtaca ttatcgagaa agcggagcag ctttagtaaa
     1741 tgcttatagc tataaaaaca ctaaagacct attagattta aataaacagg tggttatcgc
     1801 cgaaaatata tttaatattg ccgatcctaa aacaaaagcg atacgagttg caaaagatag
     1861 tgcagaatat ttaggaaaag tatcagatat tactgtaaca aaaaatgtaa ttaataataa
     1921 ttctaaggaa acagaacaac caaatattga attattacga gttagtgata atttagtagt
     1981 gtcagagaat agtatattcg gtggtaaaga aggaattgtt attgaggatt taaagggtaa
     2041 aataaccgtt ttaaataacc aattttataa tttatccggt aagtatatat cattcatcaa
     2101 atctaatgca aacgggaaag aacctgttat acgtgatagc gatggtaatt tcaatattgt
     2161 aacggagaat gggctttaca aaattgtaac aaataattta agtgataaaa acgaaaaaga
     2221 aaaaaacaaa gaggaaaaac aatctaattc aaataatgta attgatagta accagaagaa
     2281 cggagagttt aactcaagta aagataatag acaaatgaat gacaagatcg acaataaaca
     2341 agataataag acagaagaag taaactataa aatagttgga gatggcagag aaactgaaaa
     2401 tcatattaat aaatctaaag aaatagtaga tgtaaaacaa aaattaccaa agactggttc
     2461 gaacaagatt atggaactat tcttaacagt gacaggaatt ggtttacttt tgacactaaa
     2521 agggttgaag tattatggta aagataaata aaatttgttc gatacaagga agttccgtag
     2581 aaaatgagga tattgtaggt tcacaaaatc agtatttttg gattattggt ggagctacag
     2641 atttatataa ttctaaagag gagataggtt attcagtctc agaagttgta catattttat
     2701 cagaaagttt gtcggttaat tgtaaagaat caaaaactct taaacaaata tttgaaactg
     2761 ctttactcga ggttaaagat gaaatcggtt taaactcata tgaattaaca gagtataata
     2821 aaatgaaata a
//
LOCUS       DAVKJJ010000100         2639 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000100 DAVKJJ010000000
VERSION     DAVKJJ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.100"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            <1..1966
                     /locus_tag="VW144_001999"
     CDS             <1..1966
                     /locus_tag="VW144_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9601390.1"
                     /translation="KFIEIKATKYDSHSKTMIFDLYANINDIVDGLAFAGDMRLFVKD
                     DNQIKAETKIRMPEKNKETKAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYS
                     DSEKQQYLLKDNIILRKGYALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVL
                     SETTIYADSRNVEDGRSTQAVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDS
                     SKLVLFGKDDKEYTGEDKSNVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVR
                     NPEFYLRGKISDKGGFNWELRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDW
                     GMKDYKANGFPDKVTDMDGNVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNT
                     SIEYADGKSVVFNINDKRNNGFDGEIQEQHIYINGKEYTSFDDIKQIIDKTLNIKIVV
                     KDFARNTTVKEFILNKDTGEVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGEL
                     EKGYQFDGWEISGFEGKKDAGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKD
                     NPQVNHSQLNESHRKEDLQREEHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKT
                     GTASGAQTLLAAGIMFIVGIFLGLKKKNQD"
BASE COUNT         1047 a          322 c          495 g          775 t
ORIGIN      
        1 taaatttata gaaatcaaag cgactaagta tgactcacat tcaaaaacga tgatatttga
       61 tttatacgct aatattaatg atattgtaga tggattagct tttgctggag atatgagatt
      121 atttgttaaa gatgacaatc aaataaaagc tgaaactaaa attagaatgc ctgaaaaaaa
      181 taaggaaact aaagcagaat atccgtatgt atcaagttat gggaatgtaa tcgaattagg
      241 agaaggagac ctttcaaaaa acaaaccaga caatttaact aaaatggaat ctggtaaaat
      301 ctattctgat tcagaaaaac aacaatatct gttaaaggat aacatcattc taagaaaagg
      361 ctatgcacta aaagtgacta cctataatcc tggaaaaacg gatatgttag aaggaaatgg
      421 agtctatagc aaggaagata tagcaaaaat acaaaaggcc aatcctaatc taagagtcct
      481 ttcagaaaca acaatttatg ctgatagtag aaatgttgaa gatggaagaa gtacccaagc
      541 tgtattaatg tcggctttgg acggctttaa cattataagg tatcaagtgt ttacatttaa
      601 aatgaacgat aaaggggaag ctatcgataa agacggaaat cttgtgacag attcttctaa
      661 acttgtatta tttggtaagg atgataaaga atacactgga gaggataagt ccaatgtaga
      721 agctataaaa gaagatggct ccatgttatt tattgatacc aaaccagtaa acctttcaat
      781 ggataagaac tactttaatc catctaaatc taataaaatt tatgtacgaa atccagaatt
      841 ttatttaaga ggtaagattt ctgataaggg tggttttaac tgggaattga gagttaatga
      901 atcggttgta gataattatt taatctacgg agatttacac attgataaca ctagagattt
      961 taatattaag ctgaatgtta aagacggtga catcatggac tggggaatga aagactataa
     1021 agcaaacgga tttccagata aggtaacaga tatggatgga aatgtttatc ttcaaactgg
     1081 ctatagtgat ttgaatgcta aagcagttgg agtccactat cagtttttat atgataatgt
     1141 taaacccgaa gtaaacattg atcctaaggg aaatactagt atcgaatatg ctgatggaaa
     1201 atctgtagtc tttaacatca atgataaaag aaataatgga ttcgatggtg agattcaaga
     1261 acaacatatt tatataaatg gaaaagaata tacatcattt gatgatatta aacaaataat
     1321 agacaagaca ctaaacatta agattgttgt aaaagatttt gcaagaaata caaccgtaaa
     1381 agaattcatt ttaaataaag atacgggaga ggtaagtgaa ttaaaacctc atagggtaac
     1441 tgtgaccatt caaaatggaa aagaaatgag ttcaacgata gtgtcggaag aagattttat
     1501 tttacctgtt tataagggtg aattagaaaa aggataccaa tttgatggtt gggaaatttc
     1561 tggtttcgaa ggtaaaaaag acgctggcta tgttattaat ctatcaaaag atacctttat
     1621 aaaacctgta ttcaagaaaa tagaggagaa aaaggaggaa gaaaataaac ctacttttga
     1681 tgtatcgaaa aagaaagata acccacaagt aaaccatagt caattaaatg aaagtcacag
     1741 aaaagaggat ttacaaagag aagagcattc acaaaaatct gattcaacta aggatgttac
     1801 agctacagtt cttgataaaa acaatatcag tagtaaatca actactaaca atcctaataa
     1861 gttgccaaaa actggaacag caagcggagc ccagacacta ttagctgccg gaataatgtt
     1921 tatagtagga atttttcttg gattgaagaa aaaaaatcaa gattaagata aaagctatag
     1981 aaaaaaatgg tttatgtact gagattagat agtgaggtga tgacatagtt ttgtgaaaac
     2041 agccatttat aactcaatta tttagtttac ttcactattg atactatttg gagttattaa
     2101 tggacttagt ttagataact aatgaattga ttgaaagggt tagtattgac aatattggtc
     2161 atattgatta gaaaatagag tctatcaaaa tttaaaggct aatagaggtg atgagacaat
     2221 ttcaaggaat taagtagcgt ttggttgctt aattcctttt tatttggaag gaagtttttc
     2281 gtaaccgccc aataacgaag tctattggac ggttacgata tgatcactga cttcgtcagt
     2341 cttatctaca acctcaaaac tgtgttttga gcaacctgcg gctagcttct tagcttactc
     2401 tttgattttc attgagtatt agaacagaaa aaatgcttgg agtatttgtt tgtgtgtttg
     2461 tttttatata acaaactata aacaaaacaa aaatataaaa aaagagacaa aaaagaacag
     2521 aaagtaattg acaacggttt catatagtgt tatacttatg gcataaagtt aatggaaaga
     2581 agggaaaacc ttatggtatt agattatttc tttgacaaaa accttgtgtt ttgcttaga
//
LOCUS       DAVKJJ010000101         2639 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000101 DAVKJJ010000000
VERSION     DAVKJJ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.101"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            <1..578
                     /locus_tag="VW144_002000"
     CDS             <1..578
                     /locus_tag="VW144_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9601391.1"
                     /translation="SKIPSGLYKIDTGKVLFDGVNINQIDKKILSQNLGVVPQDSFLL
                     NRSILDNITLKHEVTSQKIEEVCKAVQIYDEIMAMPMKFNTIISEMGSNISGGQRQRI
                     ALARALINNPSIVILDEATSALDTINEERITKYIQSQGCTQIIIAHRLSTIKDADIIF
                     VMKGGKIVESGNHKYLMTLGGEYYSLYTKRK"
     gene            591..761
                     /locus_tag="VW144_002001"
     CDS             591..761
                     /locus_tag="VW144_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601392.1"
                     /translation="MKKENEYVILTTASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(1267..2613)
                     /gene="gdhA"
                     /locus_tag="VW144_002002"
     CDS             complement(1267..2613)
                     /gene="gdhA"
                     /locus_tag="VW144_002002"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEU9601393.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          849 a          456 c          506 g          828 t
ORIGIN      
        1 tatcaaaaat accatcagga ttatataaga ttgatacagg aaaagtttta tttgatggtg
       61 taaatattaa tcaaatagat aaaaaaatat taagtcaaaa tttaggagta gttccacagg
      121 attcattttt attgaaccga agtattcttg ataatataac tttaaagcac gaagttactt
      181 cacaaaagat agaggaagtt tgtaaagcag ttcaaatcta tgatgaaatc atggctatgc
      241 cgatgaaatt taatactatc atctcagaga tggggtcaaa tatttcaggt gggcaaaggc
      301 aacggatagc actggcacgt gcattaataa ataatcctag tattgtaatt ttagatgaag
      361 caactagtgc attagacact attaatgagg aaagaataac aaagtatata caaagtcagg
      421 gttgtactca aataattata gctcatagat tgtcaacgat taaggatgcg gatattattt
      481 ttgtaatgaa aggtggtaag attgttgagt caggaaatca taagtactta atgactcttg
      541 gtggagagta ctacagctta tatacaaaaa ggaaatgagg tgtaaagaaa atgaagaaag
      601 aaaatgaata tgtaatttta acaacagcct cactgggggt gatgattgga atagtgtttg
      661 caattttttt agattttcca gttgaatatg gtatttcttt aggcttgttg aatggaatag
      721 tattgggttc gcttattgtt tacaaaaaca ataagaatta agcataattt tttgctgtaa
      781 actaaggagt agagatggct atagttgaaa ttataaatct aacaaaaagc tttaaagata
      841 ttgaagttat tcataacact taaataatag agcaactaca gtagtagctt aaaaacatga
      901 ttaaatcgct attcttagga gtagcggttt ttctttttgt ttaatactct ttgaaaatct
      961 cttcaaacca cgtcagcttt gccttaccgt actcaagtac agcctgcggc tcgcttccta
     1021 gtttgctctt tgattttcat tgagtataaa aagggtcaag taagtatagt aaattgaaat
     1081 aagatatgaa caaatcgatt agaaaagtca aattaatttc tagaaatatg ttagaaattg
     1141 gtttgaattc cgcaatcaat ttgttcagtt tttatttcat ttcattttat ttaattagat
     1201 tttccaattt tttaattcaa gctaaaaatc cccaatcgta gtgattgagg attgagtaaa
     1261 taaatcttaa acaatacctt gtgcaatcat ggcatttgct acattttcaa aggcagcaat
     1321 gttagctcct gcaaggtagt ctttatcaag accgtatgtt tctgaagtcg ttttagctgt
     1381 gttgaagatg tttgtcatga tgtctttgag acggccatca acttcttcac gagtccatga
     1441 gaggcgaaga ctgttttggc tcatttcaag agctgaaacg gctacaccac cagcgttggc
     1501 agcttttgca ggtccgtaga agataccatt ttctttgtaa actttgatgg catcaaggtc
     1561 gctcggcatg ttggcacctt cagatacaca gataacgcct tgagcaacca aacgtttagc
     1621 tgcttcaccg ttgatttcgt tttgagtggc acatggaaga gcaatgtcat agtttccagc
     1681 gtaagtccat acagtacctt cgtggtaggt tgcagttgct ttttcagctg catactcagt
     1741 caaacgagca cgacgttttt ctttaacatc aaccaaaaga tcgaagtcga taccattttc
     1801 atcgatgaca taaccatttg agtcagaaac agaaataaca gttgcaccga gttcagttgc
     1861 tttttgaaga gcatattgag caacgttacc agaacctgaa ataacgactt tcttaccagc
     1921 aaagctgtta ccgttagctt tgagcatttc ttcagtatag taaaccaaac cgtaaccagt
     1981 tgcttctgga cgaatcaagc taccaccaaa tccaagaggt ttaccagtca agacaccagc
     2041 atcaaattgg ttaagacgtt tgtattgacc gtaaaggtaa ccaatttcac gtccaccaac
     2101 accgatatca ccagcaggta cgtcaagtga tggtccgatg tgtttttgca attcagtcat
     2161 gaagctttgg cagaagcgca tcacttcagc atctgtttta cctttaggat cgaagtctga
     2221 tccaccttta cctccaccga taggaagtcc agtcaagaca tttttaaaga tttgttcaaa
     2281 tccgaggaat ttcaagatcc cttggtttac agttgggtgg aaacgaagtc cacctttgta
     2341 tggtccaaca gctgagttga attgaacacg gtaaccacgg tttacttgaa tttttccatc
     2401 acggtcaacc caaggaacac ggaaagaaac cacgcgctca ggctcagtaa tacgtgccaa
     2461 gatattttct tcgatatact cagggtgttt ttcaaataca ggttctaaag tgttgaaaaa
     2521 ttcttcaaca gcttggagga attcagcctc gtgcccgtta cgagctttca cagtttcaaa
     2581 cacgctttgg atatattctt tagcagatgt catatcgttc tccttttatt gtcatattt
//
LOCUS       DAVKJJ010000102         2585 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000102 DAVKJJ010000000
VERSION     DAVKJJ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.102"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            22..1497
                     /gene="galT"
                     /locus_tag="VW144_002003"
     CDS             22..1497
                     /gene="galT"
                     /locus_tag="VW144_002003"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU9601394.1"
                     /translation="MKTIIDNFVDRVIEYGMYNEIDKIYVKNRVLALIGEEGIDRISD
                     ENDLKQIKDYLVEIALKNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISP
                     QEAVEEFYKLSKDSDYIKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKL
                     VKATNYPKCLLCMENEGYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYSYFNEHAIF
                     LNSQHVPMAITSKTFEQLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPM
                     EKAKFESEFCFKDFEDVNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEV
                     DIIAMTEDVPHHTVTPIARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKEN
                     IGLIEVMGLAILPPRLKPELEEVGKYLLGEDNAIADYHLERADQLKEKYPRINKEEVN
                     SVVQHEAGQVFARVLEDAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            1510..2520
                     /gene="galE"
                     /locus_tag="VW144_002004"
     CDS             1510..2520
                     /gene="galE"
                     /locus_tag="VW144_002004"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9601395.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSKLVLDRI
                     EAITGIRPVFYELDVCDKQALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGIPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWNWQSKNPNG
                     YN"
BASE COUNT          917 a          357 c          539 g          772 t
ORIGIN      
        1 ataaattaga aagaaaaata tatgaaaaca attattgata attttgttga tagagtaatt
       61 gaatacggaa tgtataatga aattgacaaa atatatgtga aaaatagagt actagcgtta
      121 ataggtgagg agggaattga tcgcatttcc gatgaaaatg acctgaaaca aataaaagat
      181 tacttagtgg aaatcgcctt aaaaaacggt aaaattaaag atttaatcga ggaaaaggaa
      241 tgtttaggag cagagttaat gaatttcata gttcctttac ctagtcgatt aaatgatatt
      301 ttttggagtt catatgatat atcacctcaa gaagcagttg aagagtttta taagttaagt
      361 aaagatagtg attatattaa aacttctgct attgctaaga atattgagtt tcgagcatca
      421 actaaatacg gagagctaga gattacgata aatttgtcaa aacccgaaaa agatccaaaa
      481 acaattgctg ctgaaaaatt agtaaaagca actaattatc ccaaatgttt actatgtatg
      541 gaaaatgaag gatatcaggg gcgaataaat tatccagcac gttcgaatca tagaattatt
      601 cggttgaaac ttggtgacga agtatgggga ttccagtatt ctccctactc atactttaat
      661 gagcatgcga tatttttgaa tagtcaacat gtaccaatgg ctattacctc taaaacattt
      721 gaacaattat tggagattgt tgatattttg ccaggatatt ttgctggttc aaattctgat
      781 cttcctatct caggaggctc cattcttagt cataaccact accagggagg gaaacatatt
      841 ttcccaatgg aaaaagcaaa atttgagagt gaattttgtt ttaaagactt tgaggatgtt
      901 aatgctggta ttgtaaaatg gccaatgtca gtaattaggt tacaaagtga aaataaaaat
      961 cgcttgttag atttagctac taaaattcta aataagtgga gagaatattc agatttagaa
     1021 gtagacatca ttgctatgac tgaagatgtc ccacaccata cggtgactcc tattgctcgt
     1081 aaagtggacg ggagatacga attagatatt gttctaagag ataatcatac tactgaacaa
     1141 tacccagatg gtgttttcca tcctcatcaa gatgtacaac atattaaaaa ggaaaatatc
     1201 gggttaatcg aagtgatggg acttgcgata ttaccacctc gtttgaaacc agagttagaa
     1261 gaagttggaa aatatctttt aggagaggat aatgcaattg cggattatca tttagaacgg
     1321 gccgaccaat tgaaagaaaa atatcctcgc attaataaag aagaggttaa tagcgtagtt
     1381 caacacgaag caggacaagt gtttgcgaga gttctggaag atgcaggagt ttataagaat
     1441 acaccgtctg ggcatgaagc gtttatgaga tttgttaagt cggttggaat taattaggag
     1501 gaaacccaga tggcaatatt ggtaacaggc ggagctggtt atattggtag ccataccgta
     1561 gtagaattac taaatttagg aaaggaagtt atcattgtcg acaacctttc gaactctagc
     1621 aaattggtgc tagaccgtat tgaagcaatt acaggaatac gtcccgtgtt ttacgaatta
     1681 gatgtttgtg ataaacaagc attgagaaag gtatttgaac aagaatcgat tgatgctgca
     1741 attcattttg caggttataa agctgtcgga gaatccgtgc aaaagcctgt gatgtactac
     1801 aaaaataata ttatgagtac attggcactt gttgaagtga tgtcagaatt taatgttaaa
     1861 aagattgtct tttcttcaag tgcgactgta tatggaatta acaatcagtc acctctaatt
     1921 gagacgatgc aaacaagtgc gacaaatcct tatgggtata cgaaagtgat gcttgagcaa
     1981 attttaaaag atgttcatgt ggcagattca gaatggagta ttgcgttgct tcgttatttc
     2041 aatccaattg gtgctcatga gtctggtttg attggggaag atccctcagg aattcctaac
     2101 aacttgatgc cttatattgc acaagtagcg gtaggtaagc tatcagagct aagtgtgttc
     2161 ggaaatgatt atgatacgct tgatggtact ggtgtgcgtg attatatcca tgtagtagat
     2221 ttagcaatag gacatataaa agctttagaa aaagtatctg aaaaaacaga tgtttatatc
     2281 tataacctag gctcgggaga aggcactagt gtattacaac ttgtaaatac atttgaaagt
     2341 gttaataaga tccctattcc ttataaaata gttccaagac gttcaggaga cgttgcaact
     2401 tgttatgcaa atgcagacaa agcatataag gaattaaatt ggaggacaac aaaatcgatt
     2461 gaagacatgt gtagagatac atggaattgg caatcaaaaa atcccaatgg ctataattga
     2521 tgatactgtc tatatgagtg ctgtattttg aaaaaattac ttaacctttt aatgaaatgc
     2581 atatt
//
LOCUS       DAVKJJ010000103         2533 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000103 DAVKJJ010000000
VERSION     DAVKJJ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.103"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            144..1340
                     /gene="tuf"
                     /locus_tag="VW144_002005"
     CDS             144..1340
                     /gene="tuf"
                     /locus_tag="VW144_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9601396.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            1724..2533
                     /locus_tag="VW144_002006"
                     /pseudo
     CDS             1724..2533
                     /locus_tag="VW144_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          822 a          495 c          501 g          715 t
ORIGIN      
        1 ttaaattttt accaagaata attcacaaaa acgttgtaaa acacttgcaa tttagctgaa
       61 atttgataaa atagtaagga aagttagact gtattgccta ctgtctatct ataaaatata
      121 ttttattgga ggcttttact caaatggcaa aagaaaaata cgatcgtagt aaaccacacg
      181 ttaacattgg tactatcgga cacgttgacc acggtaaaac taccctaact gcagctatca
      241 caactgtttt ggcacgtcgc ttgccttcat cagttaacca acctaaagac tatgcgtcta
      301 tcgatgctgc tccagaagaa cgcgaacgcg gtatcactat caacactgcg cacgttgagt
      361 acgaaactga aaaacgtcac tacgctcaca tcgacgctcc aggacacgcg gactacgtta
      421 aaaacatgat cactggtgct gctcaaatgg acggagctat ccttgtagta gcttcaactg
      481 acggaccaat gccacaaact cgtgagcaca tccttctttc acgtcaggtt ggtgttaaac
      541 accttatcgt cttcatgaac aaagttgact tggttgacga cgaagaattg cttgaattgg
      601 ttgaaatgga aatccgtgac ctattgtcag aatacgactt cccaggtgac gatcttccag
      661 ttatccaagg ttcagcactt aaagctcttg aaggtgactc taaatacgaa gacatcgtta
      721 tggaattgat gaacacagtt gatgagtata tcccagaacc agaacgtgac actgacaaac
      781 cattgcttct tccagtcgag gacgtattct caatcactgg acgtggtaca gttgcttcag
      841 gacgtatcga ccgtggtatc gttaaagtca acgacgaaat cgaaatcgtt ggtatcaaag
      901 aagaaactca aaaagcagtt gttactggtg ttgaaatgtt ccgtaaacaa cttgacgaag
      961 gtcttgctgg agataacgta ggtgtccttc ttcgtggtgt tcaacgtgat gaaatcgaac
     1021 gtggacaagt tatcgctaaa ccaggttcaa tcaacccaca cactaaattc aaaggtgaag
     1081 tctacatcct tactaaagaa gaaggtggac gtcacactcc attcttcaac aactaccgtc
     1141 cacaattcta cttccgtact actgacgtta caggttcaat cgaacttcca gcaggtactg
     1201 aaatggtaat gcctggtgat aacgtgacaa tcgacgttga gttgattcat ccaatcgccg
     1261 tagaacaagg tactacattc tctatccgtg agggtggacg tactgttggt tcaggtatgg
     1321 ttacagaaat cgaagcttaa ttcgatttag ttcccagaag aacaattatt taagtcagac
     1381 actaaaagaa tcttgcttgg caaggttctt tttttagata ttgaactaat actcaatgaa
     1441 aatcaaagag caaactataa tatattgaaa ctagaatagt acacatttac ttctaaaaca
     1501 ttgttagaaa tcgatttgac tgtcctgatc gatttgtctt gttcttattt cattttacta
     1561 tagaaagtta gctacagact gctcaaaaca ctgtttttag gttgtagata gaactgacga
     1621 agtcagtaac atctatacga caaggcgaag ctgacgcggt ttgaagagat tttcgaagag
     1681 tataatacta gactaaaatc aaaaagcatt atacaatagt aatatgaaat caattaaaga
     1741 agaaatccaa accatcaaaa cacttttaaa agactctcgt acagctaaat atcataaacg
     1801 tcttcaaatc gttctatttc gtctgatatg ggcaaatctt ataaagagat tatagaactt
     1861 ttagactgta atcaaacaat gatttggcga aatgtaaaaa aatatgagga gttcggactc
     1921 gactctctcc ttcaagaaac acgtggtggt cgtaaccatg catatatgac agttgaggaa
     1981 gagaaagtct ttcttgcccg ccatttgaag gctgcagagg caggagaatt tgttacaatt
     2041 gatgccttat ttcaggctta taaaaaggag ttaggtcgtt cctacacacg tgatgccttc
     2101 tatcaactgt tgaagcgcca tggttggcga aatattatgc cacgtccaga acatcctaag
     2161 aaagcagacg ctcaaaccat tgtcgcgtct aaaaataaaa tctcaattca agaagacaag
     2221 aaagcgtttt aaatatagta gacgttttcg taaggttcgc ttgatgtacc aagatgaggc
     2281 tggtttcggt agaatcagta aactgggatc ttgttggtct ccaataggag taggtccaca
     2341 tgtccatagt cactatatac gagaatttcg ctattgttat ggagctgttg atgcccatac
     2401 aggcgaatca tttttcttaa tagctggtgg atgtaatact gagtggatga acgccttttt
     2461 agaagagctt tcacaagctt atccagatga ttatctttta ctcgttatgg acaatgctat
     2521 atggcataaa tca
//
LOCUS       DAVKJJ010000104         2488 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000104 DAVKJJ010000000
VERSION     DAVKJJ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.104"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            83..1837
                     /locus_tag="VW144_002007"
     CDS             83..1837
                     /locus_tag="VW144_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9601397.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISAALTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIVTPILVLVLGFIVSIRVGI
                     TLLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            1821..>2488
                     /locus_tag="VW144_002008"
     CDS             1821..>2488
                     /locus_tag="VW144_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9601398.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLY"
BASE COUNT          891 a          332 c          458 g          807 t
ORIGIN      
        1 gtataataga aaagcaatga tagatattat caattcagcg aatttatatc taaaagggat
       61 attaaagaaa ggagatatgc ttatgaagat ttacaaaaaa ctatttgctt atgtccaaga
      121 taagaaatat cttggggttt tggccataat tttttctgct atatctgctg cacttacagt
      181 atatggatat tatttaatct acaaatttct agataagtta ataattaatt caaacttatc
      241 cggtgcagag agtatagcat taaaatctgt tattacacta acaagtggag cgatatttta
      301 ttttgtctca ggaatgtttt cacatatctt gggatttagg cttgaaacaa atttaagaaa
      361 aaggggaatc gatggtctgg aaaaagcaag ttttaggttc tttgacttaa atccatctgg
      421 tcaaataaga aagatcatag atgataatgc agcacaaact catcaggtgg tagctcacat
      481 gattccagat agctctcagg caatagtcac acctatactt gtccttgtac taggctttat
      541 agtaagtata agggttggta taactttgct tgctcttact ataattggcg gcttaatttt
      601 aggggcaatg atgggcgagc aagaatttat gaagatatac caagaatccc tatctaaact
      661 aagtgctgaa actgttgagt acgtgagagg aatgcaagtt gtaaaaatat ttaaagcaaa
      721 tgtagagtca tttaaaagct tttacaaggc tataaaagat tactcaaagt atgcttatga
      781 ttattcccta tcttgtaaaa ggccttatgt tttgtatcaa tggttatttt ttggactgat
      841 tgcaatttta attattccta tagtttattt tatgactagc ttagctagcg caaaggtgat
      901 tttacttgag cttatcatga ttttattttt atcaggagtt ctctttgttt cattcatgag
      961 aatgatgtgg tactccatgt atatttctca aggaaattat gcagtagata ctttagaggc
     1021 gctttacgaa gatatgcaaa aagacaaatt agtgcatggt aatgtcaata attttaaaaa
     1081 ctataatata gaatttgaga atgttagctt tgcttataat gataaagctg tcattgaaaa
     1141 tttatccttt aatttagaag aaggaaagtc ctacgcactt gtcggttcat ctggatcagg
     1201 caaatcaaca gtagcaaaac ttatatcagg tttttacaat gttaataaag gaagcataaa
     1261 gataggcggg atagcaataa gtgaatattc tgacgaagcc ttaattaaag ccatttcctt
     1321 tgtttttcaa gattcaaaat tatttaagaa gagcatttat gataatgtag ctttagctaa
     1381 taaagatgcg acgaaagatg acgttatgag agccttaaaa ttagcaggat gcgatttaat
     1441 attagacaaa ttcccagaaa gagaaaatac aatcataggc tcaaaaggtg tttatttatc
     1501 cggtggagaa aaacaaagaa ttgcaattgc tagagcaatt ttaaaggatt ccaaaattat
     1561 tattatggat gaagcatcag catctattga cccagataac gagtttgaat tgcaaaaagc
     1621 ttttaaaaat cttatgaagg ataaaacagt tatcatgatt gcacacaggc tatctacaat
     1681 taaagacctt gatgaaatta ttgtcatgga tagtggaaaa attatagaaa gagggtctga
     1741 caaagaatta atgtcaaaag atacaaggta taagagcctg caagagatgt ttaacagtgc
     1801 gaatgaatgg agggtttcaa atgaaagagt tttataaaaa aagatttgct cttacagatg
     1861 gaggagcaag aaatttaagt aaagcaacac tggcttcatt tttcgtttat tgtataaaca
     1921 tgcttcctgc catattactt atgatttttg ctcaggaagt tttggaaaat atgggcaaaa
     1981 gcaatggctt ttatatagta ttctcagttt tgactttgat agcaatgtat attttgcttt
     2041 ctatcgaata cgataaatta tataacacaa cctatcaaga aagtgcagat ttaagaataa
     2101 ggacagcgga gaatttatca aaattacctc tatcttactt ttctaaacat gacatttccg
     2161 acatttcaca aacaatcatg gctgatattg aaggcataga gcatgcaatg agccactcaa
     2221 taccaaaggt gggcggcatg gtactgtttt tcccattaat atctgtaatg atgctagcgg
     2281 gcaatgtcaa gatgggttta gctgtaatta ttccatctat tttaagcttt atatttatac
     2341 ctttatctaa aaaatatcag gttaatggac agaatagata ttatgatgtc ttaagaaaaa
     2401 actcagaaag ctttcaagaa aatatcgaaa tgcaaatgga gattaaagca tataatttat
     2461 cgaaggatat taaagatgac ttatataa
//
LOCUS       DAVKJJ010000105         2403 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000105 DAVKJJ010000000
VERSION     DAVKJJ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.105"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            233..607
                     /locus_tag="VW144_002009"
     CDS             233..607
                     /locus_tag="VW144_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEU9601399.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGFSIPRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
     gene            815..1717
                     /locus_tag="VW144_002010"
     CDS             815..1717
                     /locus_tag="VW144_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601400.1"
                     /translation="MIRKLIIFLLMLPLFVLWLELHLLVSNLQLNLEIPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEALITIFPFIFIRRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWEKEDSFESDLTNTLKDKIQKSDLSYSDLMSLQLYDAMNQSTFLTDSELFEDILSKI
                     EDSHNTLLCTGLVELLLYEISISNNNNWQEKVDKIRIQLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            1773..2012
                     /locus_tag="VW144_002011"
     CDS             1773..2012
                     /locus_tag="VW144_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU9601401.1"
                     /translation="MDLVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICAAAGVLATA
                     AGKIAYCIGYGALNRGCDINGNPYPWDGFISATVR"
     gene            2009..>2403
                     /locus_tag="VW144_002012"
     CDS             2009..>2403
                     /locus_tag="VW144_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601402.1"
                     /translation="MSNVDKIRKIHIIVCWVYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IGEDFATKLTNEYKKISQFRSKVSQSPWI"
BASE COUNT          799 a          312 c          441 g          851 t
ORIGIN      
        1 tcaaaaaaaa taaattttga aatagtatta gtgagttcgc aatcatttat tgctagtggt
       61 ttagaactgt taagtttcaa acaataattg attttaaacc ttaggtaacc tataaaaaca
      121 aataaggtag agaaaggata tcttatctag gtagctaagt gataaaaaca cagtcgtcta
      181 tcacttgtta ggaaactatc ctaactatgc ttaataaaaa aggagaaaat aaatggaact
      241 cgtattacca aataattatg ttgttcttga gcaagaagag atgatgtatc ttgatggggg
      301 attttctatt ccgagatggc ctgttgcaac agccattaat atagctttta atggtgtttt
      361 aggtggagga gcaatcagtc tagttagaaa ttatattcgt aattatggtt tgcggcgagt
      421 tacaagcgca attgctggag cagctgcaag atatgttggg gtacgagttg caaatagagt
      481 ggcaggattt gcactgtctg ctattaatgg atttgcagct tggatgtcaa ttggcgatgc
      541 tattacaaca atctgggcca acaatgatgt aaataggaga gacccaaatt taaacgcctt
      601 gtggtaacta gatatgggta ttaaaatgga aaaaattttt gttattattt tttttgtatg
      661 tctatttata tcatccataa cttttttagc ctatgatttt gttagcgaag aaataaaaaa
      721 gttgattatt tggatgaatg tcgttttttt gattttaatc atagcaatga taatttatcc
      781 aaaattaaga aaatgataag gacactgtaa aattatgata cgtaaactaa tcattttttt
      841 attaatgctc cctctttttg tcttgtggct agagttgcat ttgttagtga gtaacttgca
      901 attgaatcta gaaattcccc ttgattttgt gattagtatg agtcttgctt tctgtagttt
      961 gattttatca aaaatagttt tagacatact atacgcattg aaagatttgt acaagaaaga
     1021 agctcttatt acgatttttc cctttatttt tataagaagg aaaaaagtga atgtgagatt
     1081 ctcgccctat ttttcattcc atcgtaaaag tttgtcgcct gatgatttaa gatctaggat
     1141 tatatggagt tttattcttg aaattgctat tatcctagta tttattttaa aaattccttt
     1201 tgcaatcatc atgcttacta caatattttt ctggactata atggatatca atcatctcgt
     1261 ttttaataag acggaatttc ttttcaatca aaataaatgg gaaaaagaag attcgtttga
     1321 aagtgatttg acaaatacgt taaaggataa aatacaaaaa tcagatctca gttattctga
     1381 cttaatgtct cttcagctgt atgatgcgat gaaccaatcc acctttttaa cggatagtga
     1441 actctttgag gatattctga gtaaaattga agattctcat aacacccttc tgtgtacagg
     1501 tcttgtcgag ttactgctgt atgagatttc tattagtaac aataacaact ggcaagaaaa
     1561 ggttgataaa attagaatac agctcatcag aataaatcaa ttagattttt tctattatac
     1621 aagttggtta aggcaaaatt ttgatttctg tatgaataga gagtatcata aaatgaaatc
     1681 tcggaaatta ttactgagta ataaaaagat agtttaagtt cgtcctattt gtaaaacaaa
     1741 tgaaacaatt tcaactaagg aggagaaaac ttatggactt agtattacca aataattatg
     1801 ttgtgattga tgaagaagaa atgatgtatc ttgatggggg agcttattta agcaagcgtg
     1861 cttgtcaagg aatttgcgca gcagcaggtg tattggctac agcagcaggg aaaatagctt
     1921 actgtattgg atatggtgct cttaatagag gttgtgatat taacgggaac ccttatcctt
     1981 gggatggatt catatctgca acagtaagat gagtaatgta gataagattc gaaaaataca
     2041 catcatcgtg tgttgggtgt atatattttt atcattcagg gcgattataa atgatacgga
     2101 gtattttttg cttatttttt tagcttttat ctactcaata gtatcactac ctctatattc
     2161 tgtgaaaaat aaaatagtat ctatctgtct agccataaat tcgattctgt taatgagttt
     2221 ccccatttta atcaataagt ttttcccaga aagttttttg acttataccg tattaataag
     2281 tgtttttatc ctagagttag caatctttca tttaattggg gaagattttg ctactaaatt
     2341 gactaacgaa tataagaaaa ttagtcagtt tagaagcaag gtgtctcaat ctccttggat
     2401 aaa
//
LOCUS       DAVKJJ010000106         2216 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000106 DAVKJJ010000000
VERSION     DAVKJJ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.106"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            17..928
                     /locus_tag="VW144_002013"
     CDS             17..928
                     /locus_tag="VW144_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9601403.1"
                     /translation="MLLIGDESYSFSTLIKVLNSETVPGASFSIMEIRLPKLLAGIIA
                     GWSFGLAGFIFQTMLRNPLASPDIIGVTSSSSIAAVFCILVLKTNSLTTGIISITCGL
                     TSSLILFLLAKKDGFSAARLIILGIGFQAVTRAGTSFLLLKVARYELQEVMRWLSGSL
                     SFAKLDDIPLVLIVSIIATILVLFFNKRLEIIELGEEIAIGLGANPELSRLVLIFCAV
                     SLTAFSTSITGPIACISFLAGPIALNIGKKRSPILAGLVGILLVLLSDIFSQNILPAR
                     YPVGVVTGLLGSPYLIYLLIKMNRRNI"
     gene            928..1722
                     /locus_tag="VW144_002014"
     CDS             928..1722
                     /locus_tag="VW144_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9601404.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVSS"
BASE COUNT          819 a          337 c          367 g          693 t
ORIGIN      
        1 cagtctatca tttttcattt tacttatcgg agatgaaagt tattcttttt caactttgat
       61 taaagtctta aatagtgaaa ctgttcctgg agctagtttt tcgattatgg aaattagatt
      121 accaaaatta ttagcaggaa ttatagctgg ctggtctttt ggattggcag gatttatctt
      181 tcaaactatg ttaagaaatc ctcttgcaag tcctgatata atcggtgtca caagttcttc
      241 atctattgca gcggtctttt gcatattggt attaaaaaca aatagtttaa ctactggaat
      301 tatttcaata acttgtggac taacatcatc tttaatatta tttttactag ctaaaaaaga
      361 tggtttttca gcagcaagac tgataatatt aggtattggt tttcaagctg tcacaagagc
      421 aggcacctca tttttattgt tgaaagtagc aagatatgaa ttacaagaag ttatgagatg
      481 gctcagtggc tctttatctt ttgcaaagtt agatgacata cctcttgttc taatagtaag
      541 tattattgct actatattag ttttattttt taataaaaga ctagaaatta ttgaacttgg
      601 tgaagaaata gcaatcggac ttggagcaaa tcccgagctt tcaaggcttg ttttaatttt
      661 ttgcgctgta tctttaactg ctttttctac ttcaattaca ggaccaatag cttgtatatc
      721 ttttttagct ggtcccatag ccttaaatat tggcaagaaa agaagcccaa tattagctgg
      781 attggttgga attttactag ttttgttatc agacatattc tctcaaaata ttttaccagc
      841 tagatatcca gtaggtgttg taactggctt gttaggttca ccatacttaa tatacttact
      901 aataaaaatg aacaggagga atatataatg aaaggtctgt ggtcaaataa tcttacctgc
      961 ggttatgatg aaaaaataat cttggaaaat ataaatataa aaatacctga agaaaaaata
     1021 tcagttatta ttgggtcaaa tggttgtggg aaatcaacac tcattaaaac cttgtctcga
     1081 cttataaagc cattagaggg agaagtattg cttgataata aatcaattaa ttcttataaa
     1141 gaaaaagatt tagcaaaaca catagctata ttacctcaat ctccaataat ccctgaatca
     1201 ataacagtag ctgatcttgt aagccgtggt cgtttcccct acagaaagcc ttttaagagt
     1261 cttggaaaag atgaccttga aataataaac agatcaatgg ttaaggccaa tgttgaagat
     1321 ctagcaaata acctagttga agaactttct gggggtcaaa ggcaaagagt atggatagct
     1381 ctagccctag cccaagatac aagtatccta cttttagatg agccaactac ttacttggat
     1441 atctcatatc aaatagaact attagacctc ttgactgatc taaaccaaaa atataagaca
     1501 accatttgca tgattttgca cgatataaat ctaacagcaa gatacgctga ttacctattt
     1561 gcaattaaag aaggtaaact tgttgcagag ggaaagcctg aagatatact aaatgataaa
     1621 ctagttaaag atatctttaa tcttgaagca aaaattatac gtgaccctat ttccaattcg
     1681 cctctaatga ttcctattgg caagcaccat gttagctctt aataattgaa tatcggaatt
     1741 catttaaaag tcgcaaattt tgaactgacc ctcaaaagtt aggccaaaaa actaactaaa
     1801 ggaggtcagt tttttaatgg caaatacagc acagaattta agataaaatt agtaaaagaa
     1861 tatcatgaat gaaaaatagg atatagataa taacagcaat attaaaaaga tcaggactga
     1921 taataaacaa aaagaaagta caacgactag ttcaaaagct aaaacttcaa gtaaaaagtt
     1981 attcaagaaa atctagaaaa tactcatcct acaaaggaca ggtaggaaaa atatcagaca
     2041 acaaaataaa aagaaatatt tattaccgaa aaaaagggga aatcgagtca agtaccaaca
     2101 ggctcatcta ttccaaaaag atatttactc gatggaaaaa ctcctagaat tagtgctaca
     2161 aatattgaca atggtatctt aggttactac gaagatatag atgataaaaa ttatag
//
LOCUS       DAVKJJ010000107         2205 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000107 DAVKJJ010000000
VERSION     DAVKJJ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.107"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            1..576
                     /locus_tag="VW144_002015"
                     /pseudo
     CDS             1..576
                     /locus_tag="VW144_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            complement(810..1106)
                     /locus_tag="VW144_002016"
     CDS             complement(810..1106)
                     /locus_tag="VW144_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601405.1"
                     /translation="MLQSPYAIDTINLKKDFLEKPIDIEKFKAFLEKEEIPLAIAWQG
                     DSLHFYTKDRSILDNHLDHLLEKMVNDPEKLSDFSMDKSLDDAIDEVKSQITFK"
     gene            complement(1119..1262)
                     /locus_tag="VW144_002017"
     CDS             complement(1119..1262)
                     /locus_tag="VW144_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601406.1"
                     /translation="MSSEQQERMAVQYAEPSLLFTVRSLLKMLEWSRRQALEQDSAYK
                     IGV"
     gene            complement(1264..1551)
                     /locus_tag="VW144_002018"
     CDS             complement(1264..1551)
                     /locus_tag="VW144_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601407.1"
                     /translation="MKVVNLYDLKQMGNKGGCTIQLIHHFPFGMGLGHLKKDYIEFKR
                     VGIFDGKAVEVTLREPYSRDLLQVVKSIKQRQKLIAYRYKEGKLLFVKKEV"
     gene            2136..>2205
                     /locus_tag="VW144_002019"
     CDS             2136..>2205
                     /locus_tag="VW144_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9601408.1"
                     /translation="MKLSYEDKVQIYELRKQGYSLEK"
BASE COUNT          705 a          417 c          398 g          685 t
ORIGIN      
        1 ggcgaaatgt aaaaaaatat gaggagttcg gactcgactc tctccttcaa gaaacacgtg
       61 gtggtcgtaa ccatgcatat atgacagttg aggaagagaa agtctttctt gcccgccatt
      121 tgaaggctgc agaggcagga gaatttgtta caattgatgc cttatttcag gcttataaaa
      181 aggagttagg tcgttcctac acacgtgatg ccttctatca actgttgaag cgccatggtt
      241 ggcgaaatat tacgccacgt ccagaacatc ctatcaagaa gaaaagaaag cgctttaaaa
      301 ccagtagatg ttttcataag gttcgcttga tgtaccaaga tgaagctggt ttcggtagaa
      361 tcagtaaact gggatcttgt tgggctccaa taggagtagg tccacatgtc catagtcact
      421 atatacgaga atttcgctat tgttatggag ctgttgatgc ccatattgga agatgtcatg
      481 aatcaactcc aagatgtcat acaaggactg gagaaggagg tgataaagtc catcgttaat
      541 cggagatgga ctagaatgct ttttgaaaac agatgagtat tagaaataaa tgaaaagtca
      601 atatcaaaac aatcagaatc ttgatgatgc taaattcaaa aaaactacat aataatttta
      661 ataagggtaa gtcgtaccaa gaaaccccac caagataaaa acttagtggg gtttggcggg
      721 gaatataatt attctcccaa ttattatcgc ctttatatta tctcaactct cattaaacaa
      781 gagttccgca atcaaattgc gatactctat tatttaaagg taatttggga tttaacctca
      841 tcaattgcat cgtctaatga cttatccatg gaaaaatcag ataatttctc aggatcattc
      901 accatttttt ctaacagatg gtctaaatga ttgtctagaa tcgaacggtc tttcgtgtag
      961 aaatgcaggg aatccccttg ccaagcgatg gctaaaggaa tctcttcttt ttctaaaaaa
     1021 gctttaaatt tctctatatc aattggtttt tctaaaaaat cttttttcag attaatcgta
     1081 tcaatcgcat agggagattg tagcaattct tctaatttct acacccctat cttataggcg
     1141 gaatcctgtt ctaaagcctg acgtctagac cattctagca tctttaagag acttcttaca
     1201 gtaaataaaa gactaggctc tgcatattga actgccattc gttcctgttg ttcagaggac
     1261 atctcagacc tccttcttaa caaatagcag ctttccttct ttataacgat aagctatcaa
     1321 tttctgacgt tgcttaatcg acttgacaac ctgcagtaga tctctcgaat aaggttctcg
     1381 aagagtaact tctactgctt tcccatcaaa gataccaaca cgtttaaatt caatgtagtc
     1441 ttttttgaga tgtcctaagc ccataccaaa gggaaagtga tgaatcaatt ggatggtaca
     1501 accaccttta tttcccattt gtttcaaatc atacaagttt actaccttca tgattaactc
     1561 ctttatctag tttgtcgcat cgtttgagtc tggaaacgat aaactccgtg tctgttagaa
     1621 aattctcaca cacgtcttgt gccagttgcc cttcacaggg aaatactctc agtccctact
     1681 tacacaggca cgctaatcaa gacggagtga attcaatttt caaagaacag gtagctttat
     1741 tatagataag agtagttgaa atttttatca catctttgag gttattggaa taatcaggta
     1801 agtgctaatt tctagctctt ctcacaacac cgtacttacc ttttatgttt tatgaacagt
     1861 agctatcatt tacattttca aactaccatt ctatcagact aaaagtcaaa atagacgctt
     1921 ctacttcgag atatactcat ttaatggttc agcaattcgc tctacttctg ttacagaaat
     1981 ttcatcactt tgacttctca tgatttgtta ttagttcact ttgagggctt gctcatgaag
     2041 ccaaacagcg taagtcgtaa aagaaaaaag cagtacaaga tgaactgcac cccaaaagtt
     2101 agacagaaaa aatctacttt ttggggtgtt ttattatgaa attgagttat gaggataaag
     2161 ttcagatcta tgaactaaga aagcaaggat atagcttaga gaaga
//
LOCUS       DAVKJJ010000108         2186 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000108 DAVKJJ010000000
VERSION     DAVKJJ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.108"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            <1..1212
                     /gene="dcm"
                     /locus_tag="VW144_002020"
     CDS             <1..1212
                     /gene="dcm"
                     /locus_tag="VW144_002020"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEU9601409.1"
                     /translation="LDLFAGIGGFRMGMEAQGHECLGFCEIDKFARKSYKSIFQTEGE
                     IEFHDIRDVSDDEFKKLRGKVDVICGGFPCQAFSIAGRRLGFEDTRGTLFFEIARAAK
                     QIQPRFLFLENVKGLLNHDKGRTFTTILTTLDELGFDVEWQVLNSKDFGVPKNRERVF
                     IIGHSRKKGTRLLFPFRREGQATNSETLKALGNLNPSRSGMSGKVYYSEGLAPTLVRG
                     KGEGFKVAIPCMTPDRLDKRQNGRRFKENQEPMFTLNTQDRYGIVVVGDLPTSFKETG
                     RVYGSEGLSPTLTTMQGGDKIPKILIPEPIQFLKVREATKKGYAQAEIGDSINLERPS
                     SQYRRGRVGKGIANTLTTSGQMGVVVASYEGEDKQVYQVAGVLIDGQFYRLRIRRITP
                     KECFRLHGFPD"
     gene            1240..1350
                     /locus_tag="VW144_002021"
     CDS             1240..1350
                     /locus_tag="VW144_002021"
                     /inference="COORDINATES: protein motif:HMM:NF012372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEU9601410.1"
                     /translation="MNSQLYKQVGNSVTVPVIAAIAKKLKEIEEKDKSIK"
     gene            1334..1783
                     /locus_tag="VW144_002022"
                     /pseudo
     CDS             1334..1783
                     /locus_tag="VW144_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645893.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1776..2156
                     /locus_tag="VW144_002023"
     CDS             1776..2156
                     /locus_tag="VW144_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU9601411.1"
                     /translation="MIDLYLSKNSQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIGLPFVVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
BASE COUNT          744 a          335 c          471 g          636 t
ORIGIN      
        1 ttagatttat ttgctgggat aggaggcttt aggatgggaa tggaagctca agggcatgag
       61 tgcttgggat tttgtgagat tgataaattt gctaggaaat cttataagtc catttttcaa
      121 acggaaggag aaatagaatt tcatgacata cgagatgttt cagatgacga atttaaaaaa
      181 cttagaggga aagtcgatgt catctgtggg ggattccctt gtcaagcatt ttcaatcgca
      241 ggaagacgat tgggatttga agatactaga ggaactttgt tctttgaaat tgctcgggcg
      301 gccaaacaaa tccaaccacg ttttcttttt cttgaaaatg ttaaaggcct actcaatcac
      361 gataagggac ggacgttcac cacaatactt accacacttg atgagttggg gtttgatgtt
      421 gagtggcagg tgcttaacag taaggatttt ggcgttccca aaaacagaga gagggtgttt
      481 attatcggac attctagaaa gaaaggtacc agactcttat ttcctttcag acgagaaggt
      541 caagcaacta actctgagac tttgaaagca ctaggaaatt tgaatccatc aagaagtgga
      601 atgagcggta aagtctatta ttcagaaggt cttgcgccaa ccttagttcg tggtaaagga
      661 gaaggattta aagttgcgat tccttgtatg acaccagata gattagacaa gagacaaaat
      721 gggagacgct ttaaggaaaa tcaagagccg atgtttactt taaatactca ggatcgttat
      781 ggcattgtcg ttgttggaga tttaccgact agctttaagg agacaggtcg tgtctatgga
      841 agtgagggct tatctccaac actgactacg atgcaaggag gagataaaat ccccaaaata
      901 ctaattccag aaccaatcca atttctaaaa gtacgggaag caacgaaaaa aggatatgct
      961 caagcagaga ttggggattc aatcaattta gaaagaccaa gttctcagta tcgtcgtggt
     1021 agagttggaa aaggtatagc taatacgtta acaactagtg gtcaaatggg agtagtagtg
     1081 gctagctatg aaggagaaga taaacaagtt taccaagtag ctggtgtttt aatagatgga
     1141 caattttacc gtttgagaat acgacgaatc actcctaaag agtgttttcg attgcatggc
     1201 tttcctgatt aggcttttga agctgctaga aaagtctcta ttaatagtca gctctataaa
     1261 caagttggta atagtgtaac cgttcctgtg attgccgcaa tcgcaaagaa attaaaagaa
     1321 atagaggaaa aagataaaag cattaaataa agaatcaata ctagattgtg atgaattaga
     1381 aacagaatta catgatgcag aaatcaaaca gctggatgaa caaatatttt tgatgcccaa
     1441 ttatccatgt gagtttgagg taacattttt agatgattac cataaaaaac ataattaccc
     1501 actattttat gaatcctatc ttcaaaacgt tatggaattc cttgaaagtc aggatataaa
     1561 gaacggagtt aatgcctttg tagatgataa tcagaatctt gtttttgttt tatattgaca
     1621 agactatcga gccgagggaa aagaggggat acttacaacc caagtaactg taaaagctta
     1681 tgatgaagac aagaaatcga ttaacttctc aaagttgtta gattccttaa tcgtctcaga
     1741 atatcaaatg gaagcaaatt tgttggaggt ctcccatgat tgatctctat ctaagtaaaa
     1801 atagccaaag aaatcaactt cttttagact tcttccaaaa ctatggcatt gaggtatctt
     1861 gtcattcagt ttctgaaatg acaaaggaca aattaattga gatgatgagc tattcttcag
     1921 attgttttga gtttttatct cccaatttat tacgatttaa gaaccgtgac aatctaagat
     1981 taacggattt cgttgagata attttaaaaa atcctgaact aaccatcgga cttccttttg
     2041 tggtttcaaa taagcgggtt tatccaagtc tgaatttgga agaggctaga gctctattgc
     2101 caagagatac gaaacaattg atttacatgg cacaaacaca ttatttatct aactaaagga
     2161 gaagtaatat ggctgaacga ttttgg
//
LOCUS       DAVKJJ010000109         2099 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000109 DAVKJJ010000000
VERSION     DAVKJJ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.109"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            29..1054
                     /locus_tag="VW144_002024"
     CDS             29..1054
                     /locus_tag="VW144_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9601412.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            1044..2051
                     /locus_tag="VW144_002025"
     CDS             1044..2051
                     /locus_tag="VW144_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9601413.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
BASE COUNT          747 a          294 c          373 g          685 t
ORIGIN      
        1 agacttgaaa tatgtttaag gagtttttat gaaaaacaaa ttttttctaa tagctatttt
       61 agctatgtgt atagttttta gcgcttgttc ttctaattct gttaaaaatg aagaaaatac
      121 ttctaaagag catgcgcctg ataaaatagt tttagatcat gctttcggtc aaactatatt
      181 agataaaaaa cctgaaagag ttgcaactat tgcttgggga aatcatgatg tagcattagc
      241 tttaggaata gttcctgttg gattttcaaa agcaaattac ggtgtaagtg ctgataaagg
      301 agttttacca tggacagaag aaaaaatcaa agaactaaat ggtaaagcta acctatttga
      361 cgatttggat ggacttaact ttgaagcaat atcaaattct aaaccagatg ttatcttagc
      421 aggttattct ggtataacta aagaagatta tgacactcta tcaaaaattg ctcctgtagc
      481 agcatacaaa tctaaacctt ggcaaacttt atggagagat atgattaaaa ttgattcaaa
      541 agccttaggt atggaaaaag aaggtgatga gttaatcaaa aatactgaag ctcgtatatc
      601 caaagaatta gaaaaacatc cagaaatcaa aggaaaaatc aaaggaaaaa aagtattatt
      661 tactatgatt aatgctgcag atacatcaaa attctggatt tatactagca aagatccaag
      721 agcaaattat ttaacagatt taggtctagt tttccctgaa tcattaaaag aatttgagag
      781 tgaagatagt tttgcaaagg aaatttctgc agaagaagca aataagataa atgatgctga
      841 tgtaatcata acttatggtg atgataaaac tcttgaagct ttacaaaaag atcctctttt
      901 aggtaaaata aatgcaatta aaaatggtgc cgttgctgta attccagata atacaccgtt
      961 agcagcctca tgcactccaa caccactttc aataaactat actattgaag aatacctaaa
     1021 tcttttagga aatgcatgca aaaatgcgaa ataaaaaaca aataaaccta ggcataattt
     1081 ttgtaatctg cctaggtctt cttattacaa tatttttgtc attaaagctt ggaacaaaag
     1141 aaattaatat cagagatttt ttagcagctt ttggaatggg taatacaaat gatgatttta
     1201 ttaaatcaat tatatataaa agaataccta gaactatttt tgcaatttta gcaggttcta
     1261 gtcttgccat aagcggtgta ttgatgcaat cagttactag aaacccaata gctgatccag
     1321 gtatactcgg tataaacaca ggagcaagtc ttagtgtagt aattggtctt tcttttttag
     1381 gaatttcatc aagcataagc catataagtt ttgcaatcat tggtggctta gtaagtgcaa
     1441 tttttgtata cgcgattgct gtaagcggaa aagcaggcct tacccctata aaacttgcct
     1501 tatcaggaac ttgtgttagt atggctttaa gcagttttgt aagtttttta attttaccga
     1561 ataataacgt cttagacaaa tttagatttt ggcaaatagg tagccttgga gcagctacat
     1621 tatcttctat atctacacta ctacctttta taattttagg tcacttgata gctatattta
     1681 tttcatcaga tttaaacgct ttagctatgg gtgatgaaat ggctgttggt cttggagtta
     1741 atgttaatag gataagatca cttgcaataa ttgcaagtgt gcttttatgt tcaagtatta
     1801 ctgcaattgg tggacctatt ggcttcgtag gtcttatagt tcctcacttt tgtggcttat
     1861 ttataagcaa agatatacgc acaatgacca tttcttcatc ttttataggt gcagagctct
     1921 tgcttatatg tgatataatc ggccgtatgt taggtaaacc aggtgaaatt gaagtaggga
     1981 taattactgc aataatcggg ggtccagtac ttatttatgt aactatgaaa aatagagggg
     2041 ttaataacta atgcaaaatt taattatagg tattcaaaaa agaaaaaata gaataacac
//
LOCUS       DAVKJJ010000110         1886 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000110 DAVKJJ010000000
VERSION     DAVKJJ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.110"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(1..342)
                     /locus_tag="VW144_002026"
                     /pseudo
     CDS             complement(1..342)
                     /locus_tag="VW144_002026"
                     /inference="COORDINATES: protein motif:HMM:TIGR01716.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
     gene            complement(380..712)
                     /locus_tag="VW144_002027"
     CDS             complement(380..712)
                     /locus_tag="VW144_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9601414.1"
                     /translation="MKNYGEAFRYFRKLNGYSLEYAAADSISKSQLSRFERGENEISL
                     STFFELLSNINVSIENFCNHLEHYKRSERDDFLVNLSPNFYSLNIKGLEVIKNKQQKL
                     FEKSGKNS"
     gene            complement(725..1498)
                     /locus_tag="VW144_002028"
     CDS             complement(725..1498)
                     /locus_tag="VW144_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9601415.1"
                     /translation="MIDKVIEQYKSHDNLDIRVELHKKYSKNKLGFNNWIFSNYQITD
                     EVKVLELGCGIGELWKSNSDSIDKMKQLIVTDFSKDMVKSTKSVIGNRNNVNYEIMDI
                     QKISFENETFDIVIANMLLHHVNDIPKALSEVNRVLKTGGIFYCATFGENGVVNYLAS
                     LFKDEVNQDLENRTFTLQNGKRYLSRYFNSVDTLLYDDELQVTSIDDLVKYIQSFKGI
                     SEIGSLEEEIIRKRLESEFNNGMLIIPKEYGMFIARKES"
BASE COUNT          645 a          341 c          197 g          703 t
ORIGIN      
        1 aaatttgaat gtagattttt caaaaacttt tgtgtcatcc tttaataatt tttttaattt
       61 catttcaaag tagtctgcat atattagatg tgaattttca aggcaacaga accaaaaatt
      121 taaacaagct gacacaacta aatttctatt cttgccgatt ttttcgtaat aatgtgtacg
      181 ctctagtatt tcttttccga ccatgtaaat ataatctatt tcaaaaagat gatataaatt
      241 accaatcagc gttatctcat aggactccca tcgttccttc tggaataaat agtcatatac
      301 taaatttaga tcatctctac taactacatg attacaatct agctgatttc ataatccctg
      361 aaccataatt gtatttattt tatgagtttt tcccactctt ctcaaaaagt ttttgttgct
      421 tattttttat tacctccaat cctttaatat tcaaactata aaaatttgga gataaattaa
      481 ccaaaaaatc atcacgctcc gatcttttat aatgttctaa gtgattacaa aaattctcta
      541 tcgaaacatt gatatttgat aataattcaa aaaatgttga aagagaaatt tcattttctc
      601 ccctttcaaa tctagaaagt tgagattttg atatagaatc agctgcagca tactctaaag
      661 aatatccgtt taattttcta aaatatcgaa aagcttctcc gtaatttttc acactttcat
      721 tcccttaact ttcttttcga gcgataaaca taccatattc tttaggaatg atcaacatac
      781 cattattaaa ctcactttcc aatcttttac gtattatttc ctcttctagt gaacctattt
      841 ctgaaattcc tttaaacgat tggatgtatt taactaggtc atctatacta gttacctgta
      901 actcatcatc atagagtaat gtatcgacag agttaaaata cctactcagg taccttttac
      961 cattttgtaa agtaaacgtt ctattttcta aatcttgatt aacctcatct ttaaataaac
     1021 ttgccaaata atttacaact ccattttcac caaatgtagc acagtaaaaa attcctccgg
     1081 tttttaagac cctattcacc tcagagagag ctttaggaat atcattcaca tgatgtagaa
     1141 gcatattagc aataacaata tcaaaagttt cattctcaaa agaaatcttt tgaatatcca
     1201 ttatctcata attgacattg tttctatttc caatcactga cttcgttgat tttaccatat
     1261 ctttagaaaa atctgtaaca atcaactgct tcattttatc tatagaatca ctattacttt
     1321 tccacaattc tcctatacca catcccaatt ctagaacctt tacttcatca gtgatttggt
     1381 agttagaaaa tatccaatta ttaaatccca atttattttt tgaatacttc ttatgcaatt
     1441 ctactcgaat atcaagatta tcatgacttt tatattgttc aattacttta tcaatcattt
     1501 taaatgacct ccactctata atttggcatt atatcacaag aataaagata gtgttaaata
     1561 agttgtataa atgcaaaaaa ggaataaatc cattatagca gagttacaac acattactag
     1621 aaagagattt attcctacga ctcagtttgg catgcttaac ttcctaactc aaaaacaaag
     1681 catcgattaa tttatccgtt cttcttttga aacagatttg aactgataat tgctcgtttt
     1741 aatttaagaa gctcttttga gcaaaacttc atttttttaa gagtccaaca atttctgtct
     1801 atcgcttttc tactagcaaa aaaaactacc ttgaggcaag ttttcattta tatatctaat
     1861 ttcgtaatag tatagtaaaa tgaaat
//
LOCUS       DAVKJJ010000111         1842 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000111 DAVKJJ010000000
VERSION     DAVKJJ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.111"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(268..1032)
                     /locus_tag="VW144_002029"
     CDS             complement(268..1032)
                     /locus_tag="VW144_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9601416.1"
                     /translation="MFKLSFHSIGHVVVRNYMSFRNLFKISIVPNLIDPLFYLLAMGF
                     GVGAYLTHVNGMLYRDFVITGLIAATAMSAATAETTVNAFIQYKIEKTYDAILMTPIN
                     TSDIVVGQAIWAGIRAVIFGGIFWLISMLITAHFHVLMLLIPLILFIVGYLFGVLGLI
                     FTYLAPSREFLNYYNVLVIRPLYMFSDTFFPITSLPPILGDLTWFSPLYHATRMIRII
                     WGGEVTGLYLHLIWLIALALLITVLPMYMLHRRYYR"
     gene            complement(1025..1795)
                     /locus_tag="VW144_002030"
     CDS             complement(1025..1795)
                     /locus_tag="VW144_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9601417.1"
                     /translation="MLYLWGKERVKKLSVKNLKKQYGSYEAVKGISFELGEGECLGIL
                     GINGAGKTSTLKMIYSLTSISSGEIDILGQNIETNRRKSKSCLGIVGQEDMLDMTLNV
                     YDNLFAHGLCYGISSVELRKRIDSLLDFVQLSKHAQKMVNQLSGGMRRRLVLARALIN
                     RPDIIILDEPTVGLDIQSRNIIWDKLLELKELGVSIIITSHYMNEIEYLTDRVLMLHQ
                     GTIKEEGTVEDLLIKYDADNLETLFLGLTGTEKEDLHV"
BASE COUNT          655 a          400 c          298 g          489 t
ORIGIN      
        1 aataaacttg taatgataga gccttatcac aaaaatcaca ttcctgtctc aaaaattgcg
       61 aaaaactagt tataataaag tgaaataaaa actatacaaa ttgattaggg aatttaaaac
      121 aatttctaac aatattttaa aaactgaacc gtactatttc aacttccata aattacaata
      181 ttgataggca cagttcccat acctgcccaa attcgaataa cgtttagtat aaattagaat
      241 atgactacaa catcacaaat aattagacta tctataatat ctacgatgta acatatacat
      301 aggaagcacc gttatcaaga gcgccaaggc tatcaaccag ataagatgaa gatacaaacc
      361 tgtcacctca cctccccata taatacgaat catgcgagta gcatgataca agggagaaaa
      421 ccaagtcaaa tcaccaagta ttgggggcaa ggaagtaatg ggaaagaagg tatctgaaaa
      481 catataaaga ggacggatta ccaaaacatt gtagtaattc agaaattccc tagacggcgc
      541 caagtaggta aagattagtc ccaaaacacc aaacaaataa cccactataa ataaaatcag
      601 aggaatcaat aacatcaaga catgaaaatg cgctgtgata agcatagaaa tcagccaaaa
      661 aatcccaccg aaaatcactg cacgaatacc tgcccagata gcctgaccca ccacaatatc
      721 actcgtattg attggcgtca tcaatatagc atcataagtc ttttcaatct tatactggat
      781 aaaggcattg accgttgttt ctgcggtggc cgcagacata gcagtcgcag caattaaacc
      841 tgtaatgaca aaatcacggt agagcatacc gttaacatgc gtcaaatagg caccaactcc
      901 aaatcccatt gctaaaagat aaaagagggg gtcgataagg ttgggaacaa ttgaaatttt
      961 aaataaattt ctaaaactca tgtagttgcg taccacaacg tgtccgatgg agtgaaatga
     1021 taacttaaac atgcaagtcc tccttctctg tcccagtcaa ccccaaaaaa agcgtctcaa
     1081 ggttgtcagc atcatacttg atgagcaagt cttctacagt tccttcttct ttgatagtcc
     1141 cttggtggag catcagcacc ctatctgtca aatattcaat ctcattcata tagtgggaag
     1201 taataatgat ggaaacacct aattccttca actccaaaag tttatcccaa ataatattgc
     1261 gggattgaat gtctaaacca actgtcggtt catctaaaat gataatatca ggccgattga
     1321 tgagagctct tgccaagaca agacgtcgtc gcatcccacc cgacagctga ttcaccatct
     1381 tttgcgcatg tttgctcagt tgaacaaaat ccaagagaga atcgattctc ttcctaagtt
     1441 cgacagatga gataccatag catagaccgt gagcaaaaag attatcataa acattcaagg
     1501 tcatgtccag catatcctcc tgccctacaa tacccagaca agatttactc ttacgacgat
     1561 tggtttcaat attttgcccc aagatatcaa tctctcctga ggaaattgag gtcagtgagt
     1621 aaatcatctt taaagtggat gttttgccag caccgttgat tcccaagata cccaaacact
     1681 ctccttctcc caattcaaag ctaattccct tgacagcttc ataagaacca tattgctttt
     1741 ttagattttt aacacttaac ttcttcactc tttctttccc ccacaaatag agcatgttgg
     1801 atactttgtt aattcatctc acttttgaaa aaatgttgag gg
//
LOCUS       DAVKJJ010000112         1819 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000112 DAVKJJ010000000
VERSION     DAVKJJ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.112"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(1..953)
                     /locus_tag="VW144_002031"
                     /pseudo
     CDS             complement(1..953)
                     /locus_tag="VW144_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
     gene            complement(966..1715)
                     /locus_tag="VW144_002032"
     CDS             complement(966..1715)
                     /locus_tag="VW144_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU9601418.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYEYLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPCVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISLILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
BASE COUNT          626 a          333 c          208 g          652 t
ORIGIN      
        1 ctttgtaatt atctgaatta tatttcatcc ccaatttata tattcctact atctttggct
       61 ttcgttccaa tatttgttta actatgaact gttttctatt tgtgtttgaa atatcaatcg
      121 cttctatcac tggggcattt atttctataa attctttttt taattgttta gtatctttgg
      181 gaagacaata tcctccaaat ccaaaagaag gattattata aaaatttcca attcttggat
      241 ctaaacaaac accttttatt acaacttcag catttaaact tctcctctca gcaaaagaat
      301 ctagttcatt aaaaaaagca acacggagag ctaagaatgt gttagaaaaa agcttaattg
      361 cttctgcttc agtaggagaa actaacataa catttttaat attggcagta ctatgagtac
      421 taatcgaaag gaacaactct gcaatttttc ttccttcaac tgtctcatct ccaacaacta
      481 tgcgacttgg atataaatta tcatatatag aacaaccttc tctcaaaaat tcagggacaa
      541 aaatgatatt ttttgtatca aacagccttt ttaatttgtt tgaaaagccg aacggaactg
      601 ttgactttaa aataatcttt ccattaggtt ttaccctcag aatcttcgat accgtttgtt
      661 cgatttcata tgtattaaaa ctaccaattt tctcatcata atctgtcgga agcgcaataa
      721 tataataatc aatattattt ttaatttcag aaaatgtatc aaaaaaagta atatttaagt
      781 tattctcgca aaaaaacttc ataagctctt catttttaga tggaagaatg cccttttttt
      841 aaattattta tttttacaga atctatatca tatgcaacaa ctttatattt agatgcaaat
      901 agtaacgcgt aggccagccc aacatgcccc aaaccaatta ctgctatatt cataaaacta
      961 cttccttatt tcttaatcca aaatctaata gaataagctg ccccattcct taaatacaac
     1021 tctttaatat tgtttaaaag tttttcaact gatttccaga ttatcaaaat ctgagattta
     1081 tagcacaata ttaatgatat tctatcaata taatgttttt catcaagttc ctcttgatac
     1141 atttttaatt ctttagtttt tcccatataa ctaaccatac tactatcact tacacatggg
     1201 aagtcctcat aatatattac tttataacgc ataaattcaa gtgcccttcc aatactattc
     1261 acaaaaacat gatcaacatg gtcaccaagt gaaagcggac aatatacgac acatttgtcg
     1321 tctaaatgca ttaacagctc ttttatgata tcattcttta atgtgtcctc attttttaat
     1381 tcactataga tatgacggta tagaaaattg ccatttctat ctttcctata gagatattca
     1441 tagtacgata agtgtctaaa atcacattgt agacgttcac aagctaacct gtcttctttc
     1501 ttcctttctt caatcggata tttcccaagg ttacacaact tatgaaattg cttagcagag
     1561 ggctgtagct gttggctcaa agggtaacca gaaaatatag taataacaag tacaatttct
     1621 ccttctgaag ttaattttga aatataatca ccacaggaaa aaattgcgtc atctaaatgt
     1681 ggagataaaa agatatactt agtattgtta ctcataacca ttccctctac aatttatcta
     1741 aaaactcact aagtgtctga ttaaattcca catcatcaaa aaaattcacc ttattcttaa
     1801 taatgaatat ttcgttaaa
//
LOCUS       DAVKJJ010000113         1728 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000113 DAVKJJ010000000
VERSION     DAVKJJ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.113"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(<1..>1728)
                     /locus_tag="VW144_002033"
     CDS             complement(<1..>1728)
                     /locus_tag="VW144_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HEU9601419.1"
                     /translation="NRLTEGLRTKVDISALNVTAENIRQSVKSLETDTQNKLNQKLSQ
                     AEFEVRAGSIRQEILNATKDKASKSELTQTAEELASKIASVHLGRRNLLKGTKELARY
                     KPVSEYNGFKVIRTVAGATRYQDSYVERTVIPTAGTEYIAIFYARASENDYPVRCHFY
                     NPNTVVSSENSSGYKSRSSDGLSIIRLSTDWQLCWVKWTQTATDQAKTVIIGRHGPQV
                     GGKEGVWVEICAPAIFEGNLAGDWSPAYEDQDERVSAVESNFKQRADSLEAGVSRLTE
                     GLRTKADISSLNVTAENIRQSVKSLETDTQNKLNQKLSQAEFEVRAGSIRQEILNATK
                     DKASKSELTQTAEELSSKIASVQVGGRNYIRGTKRMMLARGLWASGTFRPSGAGTAKT
                     IDVSDSPVTGFDKAIRLTSSNARDQIGIAQDGFYISQGTYTMSCWVKGRRGQKVKLQT
                     YWQVNDNSGISPIFTLKDENWTKLSFTSARNRAGVASIGYVYLVNAEVGEYLDVLAPQ
                     LEDGSLATSSKEAPEDIEGQISTVESTFKQRANSLDAGVRSLTEGLRTKVDISSLNVT
                     AENIRQSVKR"
BASE COUNT          387 a          449 c          357 g          535 t
ORIGIN      
        1 cctcttcaca gattgccgga tattttcagc agtcacattg agtgagctga tatccacttt
       61 ggttctaagc ccttcagtca agcttctcac accagcgtcg agcgagttgg ctcgttgttt
      121 aaaggtcgat tctactgttg aaatctgacc ttctatatct tcaggagctt cttttgagct
      181 tgttgccaaa cttccgtctt ccagctgggg cgcaagaaca tctaaatatt ctccgacttc
      241 agcatttacg agatacacat agccaattga tgcgactcca gccctatttc tagcgctagt
      301 aaacgatagc tttgtccaat tttcatcctt taatgtaaag atgggcgaaa tacccgaatt
      361 atcattgact tgccaataag tttgtagctt gaccttttga cctcttctgc ctttgaccca
      421 acaagacatc gtgtatgtgc cttgcgagat ataaaatcca tcttgagcaa tacctatttg
      481 gtctctagca ttgcttgagg tcaatcgtat cgctttatca aagccagtta ctggactgtc
      541 tgatacatca atcgtctttg ccgtcccagc gcctgacggt ctaaaagtac ctgatgccca
      601 caatcctctg gctagcatca tgcgctttgt accccggata taattcctac ccccgacctg
      661 cacactcgct atcttactag agagctcctc agctgtctgc gtgagttctg acttgctggc
      721 tttatccttg gttgcattca ggatttcctg acgaatagag ccggctcgca cctcaaattc
      781 agcctgactc aacttctgat ttagcttgtt ctgcgtgtct gtctcaagac tcttcacaga
      841 ttgccggata ttctcagcag ttacgttgag tgagctgata tcggctttgg ttctaaggcc
      901 ttcagtcaga cggcttacac cagcttcgag tgaatcagca cgctgtttaa agttggattc
      961 gaccgctgag acacgttcgt cttggtcttc gtatgctggt gaccagtcac ctgcaagatt
     1021 tccctcaaaa atggcagggg cgcagatttc aacccatacc ccctctttac cgcctacttg
     1081 agggccatgg cggccaatga tgaccgtctt ggcttgatct gttgcggttt gggtccattt
     1141 aacccagcac aactgccagt ctgtcgagag acggataata gacaagccat ctgacgacct
     1201 tgacttatat ccgctgctgt tttctgatga tacaaccgtg ttaggattgt aaaaatgaca
     1261 gcgcacagga tagtcatttt cactggctcg tgcataaaag atagctatat actctgtccc
     1321 agccgttggt ataacggttc tttccacata gctatcctga tatctagttg ctcctgcgac
     1381 tgttctgata actttaaaac cattatattc actaaccggc ttgtatctcg caagctcttt
     1441 tgtgcctttc agcagatttc tgcgccctaa gtgcacactc gctatcttac tagctagctc
     1501 ctcagctgtc tgcgtaagtt ctgacttgct ggctttatcc ttggttgcgt tcaggatttc
     1561 ctgacggata gagccggctc gcacctcaaa ttcagcctga ctcaacttct gatttagctt
     1621 gttctgcgtg tctgtctcaa gactcttcac agattgccgg atattttcag cagtcacgtt
     1681 gagtgcgctg atatccactt tggttctaag gccttcagtc agacgatt
//
LOCUS       DAVKJJ010000114         1702 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000114 DAVKJJ010000000
VERSION     DAVKJJ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.114"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            10..303
                     /locus_tag="VW144_002034"
     CDS             10..303
                     /locus_tag="VW144_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HEU9601420.1"
                     /translation="MEEQIEKKEESIKQLQNQKRQLKKKLNEQERKARNKRLIEKGAV
                     FESIFEESIDLTKDEFYKLIKTLNDEEIRLNIMEILEERIDDNVEKSSKDEIT"
     gene            613..>1702
                     /locus_tag="VW144_002035"
     CDS             613..>1702
                     /locus_tag="VW144_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HEU9601421.1"
                     /translation="MADSFHFSVNIISRGKGKSAVASAAYISGEKIKNEWDGVTHDYT
                     RKEKILVKNIILPDHIPKEFNDRSTLWNKVEMAEKNSNAQLARQFIIGLPKELSLSEN
                     KNLVERYIKENLTSQGMIVDYAIHDESQDKNGNIHCHIMTIMRPINEKGEFLAKSKKE
                     YILDEKGEKVLNKNGKPKTRKVELTTWNDTGNVEKWRENFSDLCNKYLERAGAEKRVD
                     HRSFKRQNSDYLPTIHLGSAASAMERKGIETDKGNYNREIRKYNQLVKTIKEEIKTLK
                     GWIGNLLDNLSTAYEKFKDIERDKVIDNPKLFNLTNYLLTYSEIQKEKSKYLKGYAKT
                     NKEKYDFKKLTSAYSYLRKNNIETIGQLQ"
BASE COUNT          812 a          206 c          288 g          396 t
ORIGIN      
        1 agattaagta ttgaagaaca gattgaaaag aaagaagaaa gcattaagca attacaaaat
       61 caaaaaagac agttgaagaa aaaacttaat gaacaagaaa gaaaagcaag aaacaaaaga
      121 cttatagaaa aaggtgcagt gtttgaaagt atttttgaag aaagtattga tttaacaaaa
      181 gatgaatttt ataagttaat aaaaacgtta aatgatgaag aaataagatt aaacataatg
      241 gaaattttag aagaaagaat agatgataat gtagaaaaat catctaaaga tgaaataaca
      301 taattgtccc ttggttacaa gggcgcaatt atacacccta aagggtgctt gcgtcctgcg
      361 gagtcaaacc cttgcaggga gtaacaacca ttcgcttttt aaaagtcaag ggtaaataaa
      421 ctcgctaagg ctcgcccttg acttttaaaa tttgaaatga aagtaacaat taagccgaca
      481 tactgaaaca acaaaaatta attcagtagt cggctttttt aattctatca tttcaaaacg
      541 ctcattcaca aagaggatat aaaaaaacta ttaagcctcc acctaaaaca acaaagaaag
      601 gaagtgataa aaatggcaga cagttttcat ttttcagtaa acataatatc aagaggaaaa
      661 ggcaaaagtg cagtagcaag tgcagcatat ataagtggag aaaaaataaa aaatgaatgg
      721 gacggagtaa cccatgacta tacaagaaaa gaaaaaatat tagtaaaaaa tataatattg
      781 cctgatcata taccaaaaga atttaatgat aggtctacct tatggaataa agtagaaatg
      841 gcagaaaaaa attctaatgc acaactagca agacaattca taataggact accaaaagag
      901 ttatctttaa gtgaaaataa aaacctggtt gaaagatata taaaagaaaa tttaacatca
      961 caaggaatga tagtagatta tgcaattcat gatgagagtc aagataaaaa tggaaatatc
     1021 cattgtcata taatgaccat aatgcgacca ataaacgaaa aaggagagtt cttagccaag
     1081 tcaaaaaaag aatatatcct agatgaaaaa ggagaaaaag tattaaacaa aaatggaaaa
     1141 ccaaagacaa gaaaagtaga actaacaacc tggaacgata caggcaatgt agaaaaatgg
     1201 agagaaaatt tttcagatct ttgtaacaaa tatttagaaa gagcaggagc agaaaaaaga
     1261 gtagaccata ggagttttaa aagacaaaat tcagattatc taccgacaat ccatttagga
     1321 tcagcagcaa gtgcaatgga aagaaaggga atagaaactg acaagggaaa ctataataga
     1381 gaaataagaa aatataatca acttgtaaaa acaataaaag aagagataaa aacattaaag
     1441 ggttggatag gaaatttatt agataactta tctactgctt atgaaaaatt taaggatata
     1501 gaaagagata aggtaataga caaccctaaa ctttttaatc taacaaatta tctattaact
     1561 tattcagaaa ttcaaaaaga aaaaagtaaa tatctaaaag gatatgcaaa aactaataaa
     1621 gaaaaatatg acttcaaaaa gctaacaagt gcatatagtt atttaagaaa aaacaacata
     1681 gaaaccatag gtcagttaca aa
//
LOCUS       DAVKJJ010000115         1701 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000115 DAVKJJ010000000
VERSION     DAVKJJ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.115"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(667..1659)
                     /locus_tag="VW144_002036"
     CDS             complement(667..1659)
                     /locus_tag="VW144_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9601422.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
BASE COUNT          590 a          276 c          216 g          619 t
ORIGIN      
        1 actccaaaaa acttgatttt ggagtagtta taaaaactca ttcagataat ttattatttg
       61 attttgatac gattcctgaa aatataatag tagttgacca ttttgaactt acaatcgaaa
      121 aatggttaag taaaagtcaa cagttactat agtttgcaaa ttatttacag aaagaacaaa
      181 aaatgctctg aaacactgat ttcaaagcat tttttgttag tttaaaatta ctcccattct
      241 tcttttccaa acgtacaata tatccagaac cattcaaaat actagattct attttttata
      301 atatcactaa atccacctaa ttataggacg ttttcagatt tttagtcccg tcccagtccc
      361 agtaccggag aaatattgtt ttaatataat atctgttttt gtcttctaag ctcttaaaag
      421 caaaagaaca agtaaagagt caagacaagg ataaaaagtc catattaggg caaataaaaa
      481 gctttaagac agatgacaaa tctaagtcaa ataagaaaga ccatagcaaa ggggcagaga
      541 gataaatatt ggcggtcttc ggactgcctt tattttttta tccatttttc aaatcaaatt
      601 tattcagact atatatgcac atatacactt aaattcatat aaaaacatgg cttgtaaaaa
      661 attactttaa tcacaataat cgcatttaaa attgtgatgt ttgcaagcta aattacggac
      721 ttcacttgga agttttccct tgtatctttt ataatagata gaaaatttgc tggcagatga
      781 atatccaaca gattctgcta tctcttttat aggtagttca gtgtttaaaa gaagagtttc
      841 agctacattc attctttttc tttgagtgta ctctgtaatg ctttgacaat atttttcctt
      901 aaataaattt tttaatttag taccactcat tttagatatt ttttcaagcg tgccttgatt
      961 tacattcgtt gcaaaatgat catctaagaa tcttgctaca tcttcaagtg ctttatcatc
     1021 atcaatttca attttatatt tttttctatt taagtatgtg tcaattacta tacttatcca
     1081 ttcatttgcc tttgctttaa agaaaaaatc agcggcagga gcgtccatct tacaatttaa
     1141 tatttccatt gccactcttt ctaaggcctt tgtaagtatt atttgattcg gttgaagcaa
     1201 ggttgaataa aaagattctg gattaatgtt aatagatgct aaatgttttt ctattagctc
     1261 ttttttaaaa ccaatggaaa cagcaagata acaacaattc tcgtgtaata aaaaaacaaa
     1321 atcatctttt atattatcaa aatcaaaagt acataaagag tttgcggtaa tagtttgata
     1381 cggattaaac ttttctccgt ttgcactgac aatgtaactt gaataaattg aaacatagtc
     1441 tgacatacta taagtgctat tttgaactac ttcctctttg atataaaaat catgtatatc
     1501 gataatgaag ctgcctcctt cataaaacca atataggcct tctgcataag ttgaattctc
     1561 ttttttccaa caaaatgtat gacctgcact tgaatacttc tcattgtttt tacattcatc
     1621 tactttcata taatctttta caaaatcata atatgacata acatactatc ccttttagac
     1681 aatattccaa ttagccttat t
//
LOCUS       DAVKJJ010000116         1679 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000116 DAVKJJ010000000
VERSION     DAVKJJ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.116"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            <1..276
                     /locus_tag="VW144_002037"
     CDS             <1..276
                     /locus_tag="VW144_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601423.1"
                     /translation="NYLLYKVGFLGIIYDNEALRDLSKQYLNKNISELQDGSFEGGIQ
                     DAVASKQIVVINEFIACLNSRCMPSQYDRFFYKDRPYIFSRKSPIKG"
     gene            591..1568
                     /locus_tag="VW144_002038"
     CDS             591..1568
                     /locus_tag="VW144_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601424.1"
                     /translation="MKNKRYFFDTILMILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLIPFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKRDCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLNKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYMGIKHVSIRR"
BASE COUNT          567 a          224 c          279 g          609 t
ORIGIN      
        1 aattatttac tatataaggt tggatttttg ggaatcattt acgataatga agcattaaga
       61 gatttgtcaa aacaatattt aaacaaaaat atttcggaat tacaagatgg ttctttcgag
      121 ggcggtatac aagatgctgt tgcctctaaa caaatagtag ttataaatga atttatagct
      181 tgtttaaatt ccagatgcat gccatcccaa tatgatagat ttttttataa agatagacct
      241 tatatttttt ccagaaagag tcctattaag gggtgaaaat ttgttacatt tggattaaca
      301 agtgtcctag ggattagaaa taactctagt atagctagat atttttgagt aactagaaat
      361 atcattagtc tacccatatg attgccagaa aagatggctg atgaatctta tttatactgt
      421 tgcagtaggc gttgtctagg ataaataagc tatcttgatg ggggagattg gaataatttt
      481 agtagaaaca ttaaaggatt atggacaagt agcgtaggat taggtgatgc aatgtgctaa
      541 gttggcttgg tgttagcagt agtattattt aacaatggaa agtagactaa atgaaaaata
      601 agcgttattt ttttgacaca attttaatga ttttactgct tataagcacg atattttgtg
      661 tcagcccagt atttataaaa ttggacatct taggaacccc ctctcatgcc atcttaacct
      721 ttgtattggc gataccgtta ttctatattt tgtctcaatg cttacatact ttgctattac
      781 tagtttcctc aatcttttgt aaattaagac caatttattt ttattttata tttgtgatca
      841 ttataggagc taggaaatac tatcgaattc tctttcacca actaatggga tttagtccag
      901 gaatagcagt gttttataag gagagtcaaa caactaagaa tctgtttaaa ttttactact
      961 tcttgtactt tacaacacta atttcatact attttttctt tacttttgtg tatgacaaac
     1021 cgttgctttt gcctcttatt ccattttcta ttatcatagc attagtacaa aaactttata
     1081 gaattgagaa tcaacaatta tttctgttaa aatcaaaggt actaacaata ctggaatcta
     1141 agagggattg tgagtttaat cttcaggatt atcatgaaat ttggaaattg caatcaaaat
     1201 ctgagttgcc atgcgtggct ttatcttata tcagtttgat caagccatat ctttcagaga
     1261 gcgtgcgtga acaaattgat ttattagaag ttaagcgatt taaaaaaata aatcatccaa
     1321 tatcacttta tggtatgttg gatgttataa aattaaatct atatttacgg cattataatg
     1381 aaaaaaataa atatgaatca atgttaaata aaattttaga ggtaagacca gactttgttt
     1441 taatcgagca gaatattgat gatagtttaa atagctcgca accattatct ttatctttag
     1501 ccatttcaga aatccaactt ttgcttgaag tgtatatggg gatcaaacat gtttcaatta
     1561 ggagataaaa ccagaataat atttgccatt ataggggcaa gttgcatatt agtcagtatg
     1621 tatcttatac ctctcattaa cacggataat aaattttggg aaatactttt atttataat
//
LOCUS       DAVKJJ010000117         1638 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000117 DAVKJJ010000000
VERSION     DAVKJJ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.117"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            117..1214
                     /locus_tag="VW144_002039"
     CDS             117..1214
                     /locus_tag="VW144_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU9601425.1"
                     /translation="MKILHIPTGGLFSDGIGTFIYSYLEYMDLNKYDVTILATNSPLK
                     SDKEKFECLGVKVIEIERKKSNLSKYVYKLKKIIKKEKFDIIHVHGSSALMSIELLTA
                     KVAGIPVRIAHSHNTTCEHHKLDKLLRPIFYRVYTQACACSVSAGQWLFEEKNYKIIH
                     NARDVNRYQYDAIKRKKLREELLLSDETIALGHVGRFNTQKNQCFLVSLMEKLVIKNI
                     DIKLFLVGTGDTLEEIKKLVIQKKLEDNIVFLGQFDDMKSFVSSMDIMLLPSLYEGLP
                     LVSVEWQINGVESILSDRVTQECIYTGTVQQLSIDSMELWEKEILNLSTSDRERVSLQ
                     NIQLIKNAGYDISLEVKEIEELYNSLYDIAN"
     gene            1242..>1638
                     /locus_tag="VW144_002040"
     CDS             1242..>1638
                     /locus_tag="VW144_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1919 domain-containing protein"
                     /protein_id="HEU9601426.1"
                     /translation="MLQNLKPIKRKLKPIKRTYKRIVYSITKSNMRKRLKNTDFSIIS
                     DNCWGGRVYEELGLPYRTPFIGLYIFSEDYVKLLKNFRKYMEYELTFTNNSKWNTEYD
                     GEYPIGILKDIELHFLHYANQEEAYEKWNK"
BASE COUNT          622 a          193 c          298 g          525 t
ORIGIN      
        1 tcttaaagca tagtttaaat tatggatgta gattatttcg ttaactctta taatcaattt
       61 ataaggccag atagtaacat tattaaaatc atagctagga gtaagggagg atgtaaatga
      121 aaatactaca cattccaaca ggtggtttat tttctgatgg tataggaact tttatttatt
      181 catatttaga gtatatggac ttaaacaagt atgatgtgac aattttagcc actaattccc
      241 ctttgaaaag tgataaggaa aaatttgaat gtttaggggt taaagttatt gaaattgaaa
      301 gaaaaaaatc aaacctttct aaatatgtat acaaattaaa aaaaataata aaaaaagaaa
      361 aatttgacat tatacatgtt cacggaagta gcgccttaat gtcaattgaa ttactgactg
      421 ctaaagttgc tggtatccca gttcgaattg cccatagtca taacactact tgtgagcatc
      481 ataaattaga taaactacta cgaccgatat tttatcgagt ttatactcag gcatgtgctt
      541 gtagtgttag tgctggtcaa tggctttttg aggaaaaaaa ttataagatt atccataacg
      601 ctagagatgt aaataggtat cagtatgatg ccataaaacg aaaaaaactt agagaagaat
      661 tgttgctatc tgatgagact attgctctag gtcatgttgg acgttttaat actcaaaaaa
      721 atcaatgttt tctagtgtct ctaatggaaa aattagtaat caaaaatata gatataaaat
      781 tatttttagt agggacaggt gatactttgg aagaaatcaa gaaattagtc attcagaaaa
      841 aactagaaga taacattgtt tttttaggtc aatttgatga tatgaaatct tttgtgtcat
      901 caatggatat aatgttgctt ccaagtttat atgaagggtt accactagta tctgttgaat
      961 ggcaaatcaa cggagtagaa agtatcctat ctgaccgtgt tacacaagag tgtatctata
     1021 caggaactgt tcaacaatta tctatcgata gcatggaatt atgggaaaaa gaaattttga
     1081 atttatctac ttcagataga gaaagagtgt cattgcaaaa tattcagttg ataaaaaatg
     1141 caggttatga tatttcttta gaagttaaag aaatagaaga attatataat tcactttatg
     1201 atattgcgaa ttgaatgaaa gggaagagtt ttcttttgaa gatgttacaa aatttaaaac
     1261 caataaaacg gaaattaaaa ccaattaaga gaacttataa gagaatcgtt tattctatca
     1321 ctaagtcaaa tatgaggaag aggctcaaaa atacagattt ttcaattatc agtgataatt
     1381 gctggggagg acgggtatat gaagaattgg gtctgccgta tagaacacca tttataggtc
     1441 tgtatatttt ttccgaggat tatgtgaaac tgttgaaaaa cttcagaaag tatatggaat
     1501 atgagctaac ttttaccaat aattcaaaat ggaacacaga atatgacggg gagtatccta
     1561 taggcatact taaggatatt gaacttcatt ttttacatta tgctaatcaa gaagaggctt
     1621 atgagaaatg gaataaaa
//
LOCUS       DAVKJJ010000118         1637 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000118 DAVKJJ010000000
VERSION     DAVKJJ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.118"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            77..484
                     /locus_tag="VW144_002041"
     CDS             77..484
                     /locus_tag="VW144_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9601427.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATSFKEVQKPLENFN
                     HIAWFPIDEAIQNLKRGSHKWAIESWKKQHKIG"
     gene            649..948
                     /locus_tag="VW144_002042"
     CDS             649..948
                     /locus_tag="VW144_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEU9601428.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVDEANIKTLYSEKLIGISIPIK"
     gene            1209..1532
                     /gene="tnpB"
                     /locus_tag="VW144_002043"
     CDS             1209..1532
                     /gene="tnpB"
                     /locus_tag="VW144_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9601429.1"
                     /translation="MMILLSDLGQVHLVCGKTDMRQGIDSLAYVVKTHFELDPFSGQV
                     FLFCGGRKYRFKALYWDGQGFWLLYKRFENGRLTWPSTEKDVKALTSEQVDWLMKGFS
                     ITPQI"
BASE COUNT          554 a          324 c          289 g          470 t
ORIGIN      
        1 taataacgat actagataat gaggaaaatg caatggcaaa cccaactttc ggagaaaaaa
       61 agcgaataca gactatattg ctcgctatgg cgtatatgca gttatcccta atcctgaaca
      121 aaaacaaatt gttcttgttc aagaaccaaa tggtgcttgg ttcctaccat gtggagaaat
      181 tgaagcaggt gaaaatcatc aggaagccct aaagcgtgag ttgattgaag agcttggttt
      241 cacagcagaa attggtacct attacggaca agctgacgaa tatttctatt ctcgtcatcg
      301 tgatacctac tactacaatc ctgcctacct ctatgaagca acttctttca aagaagtaca
      361 aaagccacta gaaaacttta atcatattgc ctggttccct attgacgagg ctatccaaaa
      421 ccttaaacgt ggtagccata aatgggccat tgaatcttgg aaaaaacagc ataagattgg
      481 ctaatactct tcgaaaataa aattcaaacc acgtcagcgt cgccttaccg tactcaagta
      541 tagcctgcgg ctatcttcct agtttgctct ttgattttca ttgagtataa atcaagcgac
      601 tttattcaaa aagtgctata atagaattag aaaatcggag gaaatattat gaagcttatc
      661 aatacgacaa actcacactc gcaactggta aaaagtcagc tagaaagtac cgatgcaacc
      721 cttgttgagg tttattctgc agggaataca gatgttattt ttacccaagc tccgcttcac
      781 tatgaaatcc tcatctcaaa caaacaccgt gctattcgtg agcctgaaat cgaaaccatt
      841 caagaatttt tcttgaaacg taaaattgat aagacctctg ttgatgaagc caatatcaag
      901 acactctact cagagaaatt aattggaatt tcaatcccaa tcaagtaaga aaattgagta
      961 aaaactcgat ttgagagaaa gtccaataag tctttccata acaaaacgca tagtaacaag
     1021 agtttctaca cccactatta tacgttttta tcattttgaa atctgctgct aatcaatgaa
     1081 gaagcaaaga tcaaactagg cagctagtta caagttacgt aagcgcgtca taacaaggta
     1141 tctatcctaa ttccccaact tccagtatac tagataaaaa ataattatca gaggaaaggt
     1201 attgtactat gatgattcta ctaagtgatt taggtcaagt tcaccttgtt tgtggcaaga
     1261 cagatatgag gcagggaata gactcattag cctatgtagt taaaacccac tttgaattgg
     1321 atcctttctc cggtcaagtc tttctctttt gtggtggacg taaataccgc tttaaagccc
     1381 tctactggga tggtcaagga ttttggctac tatataaacg ctttgagaac ggcagactga
     1441 cttggcccag tacagaaaag gatgtcaaag ctctcacatc tgaacaagta gactggctta
     1501 tgaagggctt ttctatcact ccccaaatat agtggattta aactagaata gtacacctct
     1561 acttctaaaa cattgttaga aatcaatttg actgtcctga tcgatttgta ctgttcttat
     1621 ttcattttac tatagaa
//
LOCUS       DAVKJJ010000119         1568 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000119 DAVKJJ010000000
VERSION     DAVKJJ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.119"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            145..1491
                     /locus_tag="VW144_002044"
     CDS             145..1491
                     /locus_tag="VW144_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HEU9601430.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFR
                     MDSGFATPKLYDLIEKTGQYYLIKLKKNTVLSRLGDLSLPCPQDEDLTILPHSAYSET
                     LYQAGSWSHKRRVCQFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGRGQAENFIK
                     EMKEGFFGDKTDSSTLIKNEVRMMMSCIAYNLYLFLKHLAGGDFQTLTIKRFRHLFLH
                     VVGKCVRTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPRRKASLMMH"
BASE COUNT          459 a          346 c          298 g          465 t
ORIGIN      
        1 ctaaaaacca tccagaatcc ttgccttagc ttagatcctg gatggtttct tttttcaccc
       61 aatgggtgtt ttttactaga caaaaaagag tttccccttt atggtataag tgtagaaaaa
      121 aacacaaaaa gaaaggaaac tcacatgaac agtttaccaa atcatcactt ccaaaacaag
      181 tctttttacc aactatcttt cgatggaggt catttaaccc agtatggtgg tcttatcttt
      241 tttcaggaac ttttttccca gctgaaacta aaagagcgga tttctaagta tttagtaacg
      301 aatgaccaac gccgctactg tcgttattcg gattcagata tccttgtcca gttcctcttt
      361 caactgttaa caggttatgg aacggactat gcttgtaaag aattgtcagc tgatgcctac
      421 tttccaaaat tattggaagg agggcagctt gcttcacagc caaccttatc ccgttttctt
      481 tccagaactg acgaggaaac agtccatagt ttgcgatgcc tcaaccttga attggtcgaa
      541 ttctttttac agtttcacca gctaaaccaa ctcattgtag atatcgattc tacccatttc
      601 acaacttatg gcaagcaaga aggtgttgct tataacgccc actatcgtgc tcatggctat
      661 catcctcttt atgctttcga ggggaagaca ggttattgtt tcaatgccca gcttcgtcct
      721 ggtaatcgtt attgttctga agaggcagac agctttatca cacctgtttt agaacggttt
      781 aatcaacttc tctttcgaat ggatagtggc tttgcgaccc caaaattata cgatttaatt
      841 gaaaaaacag ggcaatacta cctcataaaa ctcaagaaaa atactgttct gagccgtctt
      901 ggagaccttt ccctcccttg cccacaggat gaggacttaa ccatcttgcc ccactccgcc
      961 tactcagaaa ctctctatca agcaggatct tggtcgcaca agcgtcgtgt ctgccagttc
     1021 tctgaacgaa aagaaggaaa cttgttctac gatgttattt ctctcgttac aaatatgacg
     1081 agtggaacaa gccaagacca gtttcagctt tatcgtggac gtggtcaagc cgagaatttc
     1141 atcaaggaga tgaaggaggg attttttggc gataaaacgg atagttcaac cttaatcaaa
     1201 aacgaagttc gtatgatgat gagctgtatc gcctacaatc tctatctttt tctcaaacat
     1261 ctagctggag gtgacttcca aactttaaca atcaaacgct tccgccatct ttttcttcac
     1321 gtggtaggaa aatgtgttcg aacaggacgc aagcagctcc tcaaattgtc tagtctctat
     1381 gcctattccg aattgttttc agcactttat tctaggatta gaaaagtcaa cctgaatctt
     1441 cctgttcctt atgaaccacc tagaagaaaa gcgtcgttaa tgatgcatta aagaacagtc
     1501 gagatgaaaa aatcgtgtga cgaaccaagg gaggagtctg cccttttgag gaaatctagc
     1561 gaggaaaa
//
LOCUS       DAVKJJ010000120         1564 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000120 DAVKJJ010000000
VERSION     DAVKJJ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.120"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(<1..713)
                     /locus_tag="VW144_002045"
     CDS             complement(<1..713)
                     /locus_tag="VW144_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601431.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIESKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREIALLLATIIVYFFTHRILPLSVFTFMFSYILLFVQ
                     SYLGSNTAWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNLTPIILLAI
                     FENLLDSYLLQNQSEVDLD"
BASE COUNT          589 a          278 c          196 g          501 t
ORIGIN      
        1 atatctaagt caacctcact ctgattctga agcaagtagg agtcaagcaa attttcaaaa
       61 atagctaaca atatgattgg tgtaaggttt ttgtattcac atgtcaagta ttctgagtat
      121 cgtgcagatg atatttcttt tatataactt tttgaaagca agattttttc aaattcatca
      181 tctattatta atcttctatt tccaatccaa gcggtattgc tacccaaata agattgtaca
      241 aatagcaata tgtaagaaaa catgaacgta aatactgaaa gaggtagaat tctatgcgta
      301 aaaaaataga cgataatggt agccaggagt aaagctatct ctcgtgacaa tcgaattctt
      361 ttcagcatct tttttaacag tccgtaaggt tgtccttggc aatattcttg aactaaatta
      421 atcggaatga catctctcac catttctgga aagtagagta agtttatagg ctgaaatctc
      481 cagctcccat cgtaagtaaa tggatataga accaaatact taaaagattt ttttcttata
      541 aacttaatta tcacaatatg aatgactgtt gcaatgaagt atattaaata taaaagtaaa
      601 accgccatca atatacttac aaaaatcccc ttggactcaa tcaatggaaa ataaaatatt
      661 gcaactagaa gcgaataaag tcccgctaat aaatatttct gatcatcttt cattcaaaac
      721 accatattat ttactaagaa tacatcaata caatagagca actctgtaag tcaatatttg
      781 ataaagcaaa gctttaaccc cttccccaga cgttagcttt tcttcaaccc actacagttg
      841 acaaggagcc caaattattg ataaaaactt tagtcatcat cgtctctata aaaatatttt
      901 ctatacactc ttgataatac caaaattaat gccaatatca taccaagagc tataataata
      961 gaatttatta gatttatttt agtatatcca atcactagtc caataattgg actagcagac
     1021 atgcttagat ataataaaaa taacaaaaaa tgaatttcac caattcctgc aaaaaaatgt
     1081 ttcatagcta tagttttacg cccataaata cccatcaaat ttaccgtcta cgatatctaa
     1141 accatatgca atggctcctc ctaatgagaa gacttcagca acagtagccg caatattaag
     1201 agcattagta atagttgcca tactaactcc accaatatat cttgttatag cacttagtta
     1261 cctagataag atatccttta gcttgcttcc aaaatttaag tctacaccta taatatgagt
     1321 tcatattagt actgctaaat cctatctata gtaatgtcaa acacctaaat aaagtcatct
     1381 ctccaattca ttcaagaaat gaagaaaggc aaaaaatctt taaaataaga aaaatgcata
     1441 ttatcaggtg ttcaaaaata ttgatgatat acgttatata gatagaaaga tttactcaat
     1501 ctcattttca aattgagttt tttcccagcc tcatttttaa tttatatctt caatagaatc
     1561 taat
//
LOCUS       DAVKJJ010000121         1496 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000121 DAVKJJ010000000
VERSION     DAVKJJ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.121"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(<1..169)
                     /locus_tag="VW144_002046"
     CDS             complement(<1..169)
                     /locus_tag="VW144_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9601432.1"
                     /translation="MEQLHFITKLLDIKDPNIQILDIINKDTHKEIIAKLDYDAPSCP
                     ECGNQLKKYDFQ"
     gene            465..623
                     /locus_tag="VW144_002047"
                     /pseudo
     CDS             465..623
                     /locus_tag="VW144_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(733..879)
                     /locus_tag="VW144_002048"
     CDS             complement(733..879)
                     /locus_tag="VW144_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9601433.1"
                     /translation="MKTKEQTRKLASGCSKHCFEVVDRTDEVSNHTHGKATLTWFEEI
                     FKEY"
     gene            893..1255
                     /locus_tag="VW144_002049"
     CDS             893..1255
                     /locus_tag="VW144_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEU9601434.1"
                     /translation="MLEVGLNSLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKISVTVIGYFEINIDENITDILYVNGTAILYPYLRSIVSIVSAIDSSEAMLL
                     PTINVLELLDKSQPFEEE"
BASE COUNT          445 a          243 c          314 g          494 t
ORIGIN      
        1 tttgaaagtc atatttcttc aattgatttc cgcactcagg gcaagatggg gcgtcgtagt
       61 ccagtttggc gatgatttcc ttgtgtgtat ccttattgat gatgtctaaa atctggatat
      121 tagggtcttt aatgtctagt aattttgtga taaaatgtaa ttgttccata tgaatctttc
      181 taatgagttg ttttgtcgct tttcattata ggtcatatgg gacttttttt ctacacaaaa
      241 taggctccat aatatctata ggggggttac ccactacaaa tattatagag cccttttttt
      301 gatgtacgac gggcatgtcg tatatctgag gtgtaagtcc tcagtgggca cctgctaccg
      361 gtgaacccaa tagcgattcc caagtctgac gcaggaaata agaatcgtca gaggaaggga
      421 tagcgaaatc gtggctctac gaacaggaac gtgataataa ggcctatata gcggataagg
      481 tatctggctg gggcgaccac tatcaattag tagctcagaa gtcggtactt aaacgtgcta
      541 tatcaaaacc agtccttgaa aaacgtgaac tggtttcgtg tttggattat taccttgaac
      601 gacatgcgtt aaaagttagt tgaactgccg tatgccgaac ggcacgtacg gtggtgtgag
      661 aggggctaga gattatcccc tactcgattt cgaaatctag tggaatgaat ctggaatagt
      721 ccatcgagct ttctaatact ctttgaaaat ctcttcaaac cacgtcaacg tcgccttgcc
      781 gtgcgtatgg ttactgactt cgtcagttct atccacaacc tcaaaacagt gttttgagca
      841 acctgaggct agtttcctag tctgctcttt ggttttcatt gagtataaca cattgttaga
      901 agttggttta aattccctaa tcagtttgtt cacatttacc ttcgatatat tatatcccat
      961 agttaaggtt ggtcatacag atgattatag tcatggagcc gtaaaactta gtgtttcttt
     1021 agttgacaaa gatgccatga aaaaaatatc tgtaactgta ataggatatt ttgaaataaa
     1081 tatagatgaa aatatcaccg atattctata cgtaaatggt actgctattc tttatcctta
     1141 tttacgttct attgtttcaa tagtttcggc aattgatagc agtgaagcaa tgttgctacc
     1201 taccattaat gttttagagt tactagataa atctcaacct tttgaagaag aataatttat
     1261 tagctcacta aattgagggt aaggaaaagt aaaagcagta agaaaaatgt cttgcattat
     1321 acagcaacct tttgggaatg agtagatgga ttgaataaaa tttgattaag agtggatgat
     1381 ttatctgtag attattattg gacagttagt cttgaagtag tctaagaatt aggttataat
     1441 cagtagaagc cttgctaata atgaggaggt tagtttatgt atagtagact gaatct
//
LOCUS       DAVKJJ010000122         1469 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000122 DAVKJJ010000000
VERSION     DAVKJJ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.122"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(33..374)
                     /locus_tag="VW144_002050"
     CDS             complement(33..374)
                     /locus_tag="VW144_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEU9601435.1"
                     /translation="MYGEGKISIGKNFHSGLNCQIITSFHNYDLGDKIPYDESYIHKD
                     VLIEDNVWVGNNVIILGGAIIEEGAIIQAGSTVIGRIPAGAIAGGHPAKPFRFRNMEH
                     YNQLKRQKKFH"
     gene            complement(523..>1469)
                     /locus_tag="VW144_002051"
     CDS             complement(523..>1469)
                     /locus_tag="VW144_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001222996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU9601436.1"
                     /translation="QYYFIVIILTFMVSGSIGVGLQSIFYPIFIYLFFNEVRDKKSIV
                     LIFDIFLYILTPLFVINTIDVFLNFTNIFHITFLGHVQVISQYSVIGFLVSAYYLLEE
                     KRNILVAQLLLILTIINCFFSDVSLSKVIALFMIVYIISYKLRKLFWKRGRKISVVTF
                     IASVVMLALVIFGYFLPYLRYFDFTFNGRYQIWRIVYATILQVKWFGYGLFGFQFKLP
                     WQKLGEVGINYTHNQVLQLALDSGIVGIISFFTMIFYMIFSTKNIQNSTISSLFIFAY
                     FCLFIIMFIESVTYYPYYFIIIVLQTLYLKLERERNVK"
BASE COUNT          566 a          257 c          175 g          471 t
ORIGIN      
        1 tttagataac ttacaatatt cattcttaac ctctaatgaa actttttttg ccgtttaagt
       61 tgattataat gttccatgtt tctaaatctg aatggttttg ctggatgtcc acctgcaatt
      121 gcacctgctg gaattcttcc aataactgta cttcctgctt gaatgattgc accttcctca
      181 ataatcgcac cacctaaaat tatcacatta tttcctaccc agacgttatc ttcaattaag
      241 acgtctttat gtatataact ctcatcataa ggaattttat cgcccaagtc ataattatga
      301 aaggaagtga taatttggca attcaatcct gaatgaaaat ttttaccaat gcttattttc
      361 ccttcaccat acattgacat accattaaat gaaacatttt catcaaggta cgtatttgca
      421 gtaacaaacg ttttgcctcc cgtatacact ctcccaccgc attgcgctgc ctttcttttt
      481 atacgataac tataatatac tttattaaaa tttcttatca atctatttaa catttctttc
      541 tctttccaat tttaagtaca aagtctgaag aactattata ataaaatagt atgggtaata
      601 agtgacactc tctataaaca ttataataaa tagacaaaaa taggcaaaaa taaatagaga
      661 gcttattgtg gaattttgaa tatttttagt tgaaaagatc atataaaaaa tcatagtaaa
      721 gaatgagata atcccaacaa taccactatc aagtgctaac tgtaaaactt gattatgagt
      781 gtaatttatt ccaacttctc ccaatttctg ccacggaagt ttaaattgaa agccaaacaa
      841 accatatcca aaccatttca cttgaagaat ggtagcatac acaatgcgcc aaatttggta
      901 tcgaccatta aaagtaaaat caaaatatct aagatatggt aaaaaatatc caaaaattac
      961 taatgctaac atcacaacag atgcaataaa agttacaaca gatatcttcc ttcctctttt
     1021 ccagaatagt tttcttaact tgtatgaaat aatataaaca atcataaaca aagcaataac
     1081 tttagagaga gagacatctg aaaaaaaaca attgataatc gttaaaatca acagcaactg
     1141 tgccactaat atatttctct tttcttctaa taagtaatat gctgatacga gaaatccgat
     1201 aacactatat tgggaaatca cctgaacatg tcctaaaaaa gtaatatgaa aaatatttgt
     1261 gaaattcaga aaaacatcaa tcgtattgat aacgaataaa ggagtaagta tataaagaaa
     1321 gatatcaaaa attaacacta ttgatttctt atctcgaact tcattaaaaa ataaatagat
     1381 gaatattgga tagaaaatcg attgtagccc aacaccgatt gacccactta ccatgaaagt
     1441 taaaataatt acaataaaat aatattgta
//
LOCUS       DAVKJJ010000123         1449 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000123 DAVKJJ010000000
VERSION     DAVKJJ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.123"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            1..>1449
                     /gene="pcpA"
                     /locus_tag="VW144_002052"
     CDS             1..>1449
                     /gene="pcpA"
                     /locus_tag="VW144_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEU9601437.1"
                     /translation="MLSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSIIQQNNI
                     KYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVASQAFS
                     YYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRAFDFSE
                     LEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLTLPKSV
                     KTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESYKTPKG
                     TKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERLAFYGN
                     LELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEIHIKNK
                     STEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETDVAKPK
                     KNSNQGVVGWVKDKGLWYYLNESGSMATGWVKD"
BASE COUNT          556 a          200 c          242 g          451 t
ORIGIN      
        1 atattatcat taactacagc tgcagttatt ttagcagcat atgtccctaa tgaaccaatc
       61 ctagcagata ctcctagttc ggaagtaatc aaagagacta aagttggaag tattattcaa
      121 caaaataata tcaaatataa ggttctaact gtagaaggta acataggaac tgttcaagtg
      181 ggtaatggag ttactcctgt agagtttgaa gctggtcaag atggaaaacc attcacgatt
      241 cctacaaaaa tcacagtagg tgataaagta tttaccgtta ctgaagtagc tagtcaagct
      301 tttagttatt atccagatga aacaggtaga attgtctact atcctagctc tattactatc
      361 ccatcaagca taaaaaaaat acaaaaaaaa ggcttccatg gaagtaaagc taaaactatt
      421 atttttgaca aaggtagtca gctggagaaa attgaagata gagcttttga tttttctgaa
      481 ttagaagaga ttgaattgcc tgcatctcta gaatatattg gaacaagtgc attttctttt
      541 agtcaaaaat tgaaaaagct aaccttttcc tcaagttcaa aattagaatt aatatcacat
      601 gaggcttttg ctaatttatc aaatttagag aaactaacat taccaaaatc ggttaaaaca
      661 ttaggaagta atctatttag actcactact agcttaaaac atgttgatgt tgaagaagga
      721 aatgaatcgt ttgcctcagt tgatggtgtt ttgttttcaa aagataaaac ccaattaatt
      781 tattatccaa gtcaaaaaaa tgacgaaagt tataaaacgc ctaaggggac aaaagaactt
      841 gcatcatatt cgtttaataa aaattcttac ttgaaaaaac tcgaattgaa tgaaggttta
      901 gaaaaaattg gtacttttgc atttgcagat gcgattaaac ttgaagaaat tagcttacca
      961 aatagtttag aaactattga acgtttagcc ttttacggta atttagaatt aaaagaactt
     1021 atattaccag ataatgttaa aaattttggt aaacacgtta tgaacggttt accaaaatta
     1081 aaaagtttaa caattggtaa taatatcaac tcattgccgt ccttcttcct aagtggcgtc
     1141 ttagattcat taaaggaaat tcatattaag aataaaagta cagagttttc tgtgaaaaaa
     1201 gatacatttg caattcctga aactgttaag ttctatgtaa catcagaaca tataaaagat
     1261 gttcttaaat caaatttatc tactagtaat gatatcattg ttgaaaaagt agataatata
     1321 aaacaagaaa ctgatgtagc taaacctaaa aagaattcta atcagggagt agttggttgg
     1381 gttaaagaca aaggtttatg gtattactta aacgaatcag gttcaatggc tactggttgg
     1441 gttaaagac
//
LOCUS       DAVKJJ010000124         1320 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000124 DAVKJJ010000000
VERSION     DAVKJJ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.124"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            <1..859
                     /locus_tag="VW144_002053"
     CDS             <1..859
                     /locus_tag="VW144_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEU9601438.1"
                     /translation="KFFTFDNDMLRKYRKDQKHFYLTHGCFPLKSTIGKITVPNYVDF
                     VLSPSGNVDNIIVKQFGLTSKEQCLHTGFPCHDIFYSKPEPLVFLSNYDKKIIWAPTF
                     RKGGGYNRNDSTASYPLGIPLLETLDELANLNTYLSQRDIALIVKLHPMQDISDLELK
                     QFSHIKFLTNQDLNRKNTNLYQLLMNSDALLSDYSAISYDYLHLDKPIGYVFSDLNDY
                     KLGLSVDNVDDYIAGDKILSFNNLLHFIDNIYLEIDKNKEKRKELFNKIFEKQDGESC
                     ERLAQIMEL"
     gene            876..>1320
                     /locus_tag="VW144_002054"
     CDS             876..>1320
                     /locus_tag="VW144_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEU9601439.1"
                     /translation="MSVGMKAALWYTVANFIQKGFVFLSTPILTRILNVNDYGTLILY
                     QSWFVLFAIFATLNLSQTAYNKGLVEFENDKDNFTFSLLFLSKTITIIVAFLYFTFNI
                     LVKDSTGLTPSFMIFLFSDILFNISIEFYLARQRFEFKYRKAVLIS"
BASE COUNT          449 a          181 c          214 g          476 t
ORIGIN      
        1 taaattcttt acttttgata atgatatgct tcgtaaatat agaaaagatc aaaaacactt
       61 ttatttaaca catggatgct ttcctttaaa aagtacaata ggtaaaataa cagttccgaa
      121 ttatgttgat tttgttctat ctccctcggg caatgtagat aatattatag ttaagcagtt
      181 tggattaaca tctaaagagc aatgtcttca cactggattt ccgtgtcatg atatctttta
      241 ttcaaaacca gaacctctag tatttttatc taattatgat aaaaaaatta tctgggcacc
      301 aacttttaga aagggtggtg gctataatag aaatgatagt acagctagct atcctttagg
      361 cattccgtta cttgaaacac tagatgaact ggcaaaccta aacacatatc taagccaacg
      421 ggatattgcc ctgattgtaa agttacatcc aatgcaagat atttcagatt tggagttgaa
      481 acaatttagt catattaagt ttctgacaaa tcaagatttg aatcgtaaaa atacaaatct
      541 gtatcaattg ctgatgaatt cagatgcttt actcagtgac tattctgcaa tctcgtatga
      601 ttatttacac cttgataaac caatagggta tgttttttcg gatctaaatg attataagtt
      661 ggggctttca gttgataatg tagatgatta catagcagga gataagatac tgtcttttaa
      721 caacttgctg cactttattg ataacattta tctagaaatt gataaaaata aagaaaaacg
      781 caaggaatta tttaataaaa tatttgaaaa acaggatgga gaatcgtgtg aaagattggc
      841 acaaattatg gaattataaa agccgttacg aagaaatgtc tgtcgggatg aaagcggcac
      901 tctggtatac tgttgcaaat tttattcaaa agggttttgt atttctttca actccgattt
      961 taaccagaat acttaatgtt aatgattacg gtacattgat actttaccaa tcatggtttg
     1021 tactatttgc tatttttgcg accttgaatc tttctcaaac agcttataat aagggattgg
     1081 tagaatttga gaatgataaa gataatttta cattttcttt gttgtttttg tctaagacta
     1141 ttactataat tgttgctttt ttatatttta cttttaatat actggttaaa gatagtactg
     1201 gattaactcc cagtttcatg atatttttat tcagtgatat cttatttaat attagtatag
     1261 aattttattt ggcacgtcag agatttgaat tcaaatatag aaaagcagtt ctaatttcaa
//
LOCUS       DAVKJJ010000125         1258 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000125 DAVKJJ010000000
VERSION     DAVKJJ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.125"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            <1..542
                     /locus_tag="VW144_002055"
     CDS             <1..542
                     /locus_tag="VW144_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9601440.1"
                     /translation="GESGSGKSTLLKLLNGFLRDYSGELRFCGDDIKKTSYLNMVSNV
                     LYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNILDYYVGDDGRL
                     LSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDREDLTVIIVTHAP
                     HPELKQYFTKIYQFPKDFI"
     gene            562..1258
                     /locus_tag="VW144_002056"
                     /pseudo
     CDS             562..1258
                     /locus_tag="VW144_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813546.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          442 a          134 c          239 g          443 t
ORIGIN      
        1 taggtgaaag tggaagcggg aaatctacat tattaaaatt attgaatggc tttttgagag
       61 attatagtgg agaattgcga ttctgcgggg atgatataaa aaaaacctcc tatttaaata
      121 tggtttcgaa tgttctatat gtagatcaaa aagcttattt gtttgaaggt acgattagag
      181 ataatatttt attggaagaa aattatactg atgaagaaat actacagtct ttagagcaag
      241 ttggtttgag tgtaaaagat tttcctaata acattttaga ttattatgtt ggtgatgatg
      301 ggagattact gtcaggaggg cagaaacaaa aaattacttt agctagaggg ctaattagaa
      361 ataagaaaat agtattaatt gacgagggaa cttctgctat cgataggaga acttcgttag
      421 cgattgaacg taagatatta gatagagagg atttgactgt cattattgtt acccatgctc
      481 cgcatccgga acttaaacaa tattttacta agatatatca atttccaaag gattttattt
      541 aagaatatag gggagggaaa catgagattc tattgtgaaa cttatgaggt aattactgaa
      601 gagaaaaaag tatcattttt ttatgtagat gaaaaaccct taaggtatct gttatttgac
      661 tatatatttc tactagttct agctaaaaaa aatatcccaa tcttagatgg agtttccaat
      721 aagcaagttg ctccaacgtt gctgcatcac ttttctttgg aaattgaaaa gaattttatc
      781 gatttttgct ataaaaatat ggatctcatc ctaaaaactc aaagtatatc tttgtgtcat
      841 agagaagagc ttattattgt agatgtttca gatgactcta aatttggtgt tttttacaag
      901 cgttttagaa cactggttga taaaaataac ggaaaatatg tttgtgttta taatttcagt
      961 cgtgtttcag atatactgca aaactggaaa aattattgca atcgaaaatt ttcggttacg
     1021 tttactgaaa gccaatttga attatttaag cttttatata atcaaaaaaa ttttaaaaca
     1081 atttcgttgc tatttggaaa aaaattgttg ctggaggaat tatctattat agcgatctta
     1141 ctaatataga atacttttgt attttttggt gggatagtat gtttgggaaa gattctattg
     1201 gaaaatacgt atatgtagaa gagattagta gatgtcattt tttagataga aattattc
//
LOCUS       DAVKJJ010000126         1201 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000126 DAVKJJ010000000
VERSION     DAVKJJ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.126"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            202..>1201
                     /locus_tag="VW144_002057"
     CDS             202..>1201
                     /locus_tag="VW144_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEU9601441.1"
                     /translation="MTKVRINWVDFGKGFAIFLVLVGHVFIGLSESNKFSIANDVLLF
                     LIAQIYIFHIPVFFALSGYFFRPVSDLKEFWYYAKKKTIILGIPYIFYSIIHFCLQKL
                     AGASVRVPTTIHNLLNIYRYPLGVSWYLYTLWSILIVYGLLSIVFKNRKSLFLVSVFA
                     YIFTLFIQIDIFIVQRTLVWGICFFLGSVLSEIHFDKINLKKFLFFFVLFDFIYMFAW
                     FLFYEVGSKKDYVSYINPGLWGIAFIVCVLVAFAIFPKMEKNFPKTFLYFTKYGKDSL
                     GIYILHAPICSMIRILMLKVGINSVFLHVVVGIVLGWYLSILATYILKKIPFLNIVL"
BASE COUNT          353 a          130 c          215 g          503 t
ORIGIN      
        1 tatgggaagt tagtagatat gaatgtgata tatagctaga attaatatta ttcattgcta
       61 tttaaaatgt aggtaagaat gtattagatt tagcataaaa ttagaaaaca agctattttt
      121 tctgtgaaat taggtagata tacaataata tttgtattgt tagagaaaga aatcttaata
      181 ttgtaatggg ggcattttga attgactaaa gtaagaatta attgggtaga ttttgggaaa
      241 ggatttgcaa tattcttagt tctagtaggg catgtattta ttggtttatc tgaatccaat
      301 aagttttcaa ttgcaaatga tgtgttatta ttcctgatag ctcaaatcta tatatttcat
      361 ataccagtgt tttttgcgtt atcaggatac tttttcagac ctgtatcaga tttgaaggaa
      421 ttttggtatt atgctaaaaa gaagacaatt attttaggca taccatatat tttttattca
      481 attattcact tttgtcttca aaagcttgct ggtgcttctg tgagagtacc cactacgata
      541 cataatctat taaatatata tagatatcct ttgggagttt catggtatct atatacgcta
      601 tggtcgatat tgatagttta tggtttacta tctattgttt tcaaaaatcg taagtctctc
      661 tttttagtta gtgtttttgc ctatattttt acactattta ttcaaattga tatttttatt
      721 gtacaacgga cgttggtttg ggggatttgt ttctttcttg gcagtgtatt gagtgaaatt
      781 cactttgata aaattaattt gaaaaaattt ctttttttct ttgtgttatt tgattttatt
      841 tatatgttcg cttggttctt gttttatgaa gtagggtcta agaaggatta tgtaagctat
      901 attaacccag gtttgtgggg gattgctttt attgtctgtg tattagttgc ttttgcgatt
      961 tttcctaaaa tggagaaaaa ttttcctaaa actttcctat atttcactaa atacgggaaa
     1021 gatagtttag ggatctatat tcttcatgca ccaatttgta gcatgattcg gattctaatg
     1081 ttgaaagtgg gaataaactc agtttttctt cacgttgttg ttgggattgt gctaggctgg
     1141 tatttatcca tactagcaac ttatatattg aaaaaaattc catttttgaa tattgtttta
     1201 t
//
LOCUS       DAVKJJ010000127         1085 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000127 DAVKJJ010000000
VERSION     DAVKJJ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.127"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            137..319
                     /gene="rpmF"
                     /locus_tag="VW144_002058"
     CDS             137..319
                     /gene="rpmF"
                     /locus_tag="VW144_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU9601442.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKITKAASAE"
     gene            335..484
                     /gene="rpmG"
                     /locus_tag="VW144_002059"
     CDS             335..484
                     /gene="rpmG"
                     /locus_tag="VW144_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9601443.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(976..>1085)
                     /locus_tag="VW144_002060"
     CDS             complement(976..>1085)
                     /locus_tag="VW144_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9601444.1"
                     /translation="TVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWVG"
BASE COUNT          389 a          197 c          171 g          328 t
ORIGIN      
        1 tgccgtgtcg gataggagaa ttttttcatc ttttttgtca agtaacttta ctttacaaaa
       61 aaaatgtgtt atcctagtat ggttgatgaa aatcagtaga ttgaatcgaa tataaatacc
      121 taaaggagaa atcaaaatgg cagtacctgc acgtcgcact tcaaaagcga agaaaaacaa
      181 acgtcgtaca cactacaaag taacagctcc atctgtaaac tttgacgaaa ctactggaga
      241 ttactcacgt tctcaccgtg tatcacttaa aggatactac aaaggacgta aaatcactaa
      301 agctgcatca gctgaataat agaagggaga taccatgcgc gtaaatatta cacttgaaca
      361 caaagaatct ggtgaacgct tgtaccttac ttctaaaaac aaacgtaaca ctccagaccg
      421 ccttcaattg aagaaatact caccaaaact tcgcaaacac gttgtgttta cagaagttaa
      481 gtaagcattc aatacgaatc aatacagaag aaaaacgctt gatcagcgtt ttttcttttt
      541 gttaatttca ctatattgca agggaaatca ttcgaatgac tacatttttc actacctttt
      601 ttgaaataag aatacggatt cggacgatta aaagtaagag aaactcaaat gtaagttggg
      661 gttccttttt tgtatatttt tcaaataacg cctctacact atttgtagca aattcaccaa
      721 ctacagttgt atcttagtta aaataagtta gaatatgtaa gtgagtacca gatataccaa
      781 gacatcgtca ccatctaagg tatattcaaa atacaaaagt tgaccaacta gatttctgaa
      841 tatccttata tatccattct taaaattggt ttaaatagcg tagtctttta aactagtttt
      901 gagaatccaa aaaatcttcc tacatatgta agaagatttt ttagttcaga atgattagat
      961 ttagctaatg gatacctatc ctacccactc accgttagca ttgactcgat agccatcggg
     1021 tgtagtcgtg tttactaata aatctcctga attataggta tagtaccatt taccagaaac
     1081 tgtaa
//
LOCUS       DAVKJJ010000128         1076 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000128 DAVKJJ010000000
VERSION     DAVKJJ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.128"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            1..1076
                     /locus_tag="VW144_002061"
                     /pseudo
     CDS             1..1076
                     /locus_tag="VW144_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISSpn4 family transposase"
BASE COUNT          381 a          190 c          239 g          266 t
ORIGIN      
        1 tcaaaaaagg gaaaaatcga tactattctc cagaactaaa acaagaaatg attgataaag
       61 ttttacatga aaactggtct caagatagag tttctctcaa atatgctctc ccaaatcgtg
      121 gtatgcttcc aaattggctg gcgcaataca agaaaaacgg gtatactatt gttgagaaaa
      181 caagagagag agtacctaaa atgggacgta aacgtaagaa aacttgggaa gaaatgacag
      241 aactagagcg acttcaagag gagaatgaac gcttacgtac cgaggtggct tacttaaaaa
      301 agttaaaaga gctggaagac agggacgaag ccctagagcg agaaaggcag agatagttag
      361 agaaatggct tcaggaggat ttcaattaga tttacttctt gaaacagcac gtttagctcg
      421 ctcgacttac tactatcagt tgaagggact aggtgggctt gacaaagata aagatcttaa
      481 agctgaaatt caagctattt ttactgagca caaaggaaat tatggctatc gtagagttac
      541 tcttgaacta agaaatcgtg gttttgtggt gaatcataag aaagttcaac gtctgatgaa
      601 agttcttggt ttaacagctc gaattcgtcg gaaacgaaag tattcctcct accaaggaga
      661 gattggcaag aaagcagaga atctcattca aggccagttc gaagcatcga gaccaatgga
      721 aaagtgctat acggatgtga cagagtttgc cattccagca agcagtcaaa agctctattt
      781 atcgccagtt ttagatggct ttaacagcga aattatcgcc tataatcttt caacttcacc
      841 aaacttagaa caagggaaag ctatgttaaa ccaggccttt acagagaaac attatgagaa
      901 taccattctc catagcgatc aaggctggca ataccaacac gattcttatc atcggttcct
      961 agagagtaag ggaattcaag catccatgtc acgcaaggga aacagcccag acaatggcat
     1021 gatggagtcc ttctttggca ttttgaaatc cgaaatgttt tatggttatg agaaga
//
LOCUS       DAVKJJ010000129         1066 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000129 DAVKJJ010000000
VERSION     DAVKJJ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.129"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(<1..1049)
                     /locus_tag="VW144_002062"
     CDS             complement(<1..1049)
                     /locus_tag="VW144_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9601445.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     YKYGDKEILKNLNFC"
BASE COUNT          378 a          184 c          134 g          370 t
ORIGIN      
        1 aaacaaaaat ttaagttttt taatatttct ttatctccat acttatagca aatatttttt
       61 gcttcatata acggaaaatc tctattcacc tcatttggtt cgatatcatt cattttattt
      121 gactcaattg gattaattga atacaatttt aaaaaaatag gcttcgtacc aataatagag
      181 gataattgac ctcctaattc acctagcgct gtaaaaataa cacctgttag tgctcctatt
      241 gcttcaatag taccaatttt cactattcct tttattgcaa gatagcctgt taaaaaaacg
      301 agagatatct gaaaaaaaat attgagaaag aagctaatag cgcctgctaa cgtttctaca
      361 gttgtctttc tttgtataac catctttaat aaaattcctg cttctttaat tttcttaggc
      421 aatacatata aaagattcaa ggacgctaac acatcaaatc cattcaatat agtctcacta
      481 gattttaaaa aagcttcatt ttggttagtt aaatttagac taacttctcg cattttcgat
      541 gcaaagattt ttggtacaag tagcataatc attaatgaaa acaaggtggc tacagtcaat
      601 gaccaatgat agtgattaag agtcacaact gcaaatatag taccagaaat tccttttatt
      661 actaaaaaaa gttgtttaaa agcctgatca tttaaagtct gaacatcatt atttagccac
      721 gaaagatatg ttcctgatga tttactatga aattcttgat aggtagagtt agagatgtct
      781 gtggcaactc tatttcgaat ctctagatta aactcttgga tcacttcaac ctgataattt
      841 ttcactaccc agtcaaggaa tattatccca caccagacaa tcatttggta gattgacaat
      901 ttcaaaaacc gctctaaatt catcgcaatt aattcattca acaccagagc attaatagtt
      961 gctgcataaa ttagcaataa ttgaccagca acaataaata tcgttaataa actaaatttt
     1021 tttatatttg attttataat agtatacaca atagtttctc actttc
//
LOCUS       DAVKJJ010000130         1058 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000130 DAVKJJ010000000
VERSION     DAVKJJ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.130"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            <1..559
                     /locus_tag="VW144_002063"
     CDS             <1..559
                     /locus_tag="VW144_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9601446.1"
                     /translation="NKDETSSYPIYKIPEAWRYIKFASLVNFRIGKTPPRSEATFWGT
                     EIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIP
                     ATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPI
                     SNHEEMKRIISKVDLLFQKVSQLFE"
     gene            complement(507..1052)
                     /locus_tag="VW144_002064"
     CDS             complement(507..1052)
                     /locus_tag="VW144_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9601447.1"
                     /translation="MNWVVIKIKDIFSMNTGLSYKKGDLSINNKGVRIIRGGNIKPLE
                     FSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGFIF
                     QLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFE
                     EQELITQKVEKLFEKVNQLWK"
BASE COUNT          381 a          170 c          153 g          354 t
ORIGIN      
        1 gaacaaagac gaaacatcct cttatcctat atataaaatc ccagaagcat ggagatatat
       61 taaatttgct agcctagtta acttcagaat aggaaaaact cctccacgca gtgaagctac
      121 tttttgggga actgaaatac cctgggtatc tatatcagat atgcctatat ctggttatgt
      181 aactaatacg agagaatcta tttctaaatt agcattaaaa tcaaagaaaa tagacatttc
      241 acctaaagga actttattaa tgagctttaa attatctatt ggaaaagttg ccatattgga
      301 tattcctgcc actcataatg aagccattat atcgatattc ccctatgcta ataaagaaaa
      361 tattatcaga gattacttaa tgatattttt gccacttatc tctactttag gtgattcaaa
      421 agatgctatc aaagggaaaa cattaaatag taccagtatc tccgaattat taatccctat
      481 ttctaatcat gaagagatga aaagaatcat ttccaaagtt gatttacttt ttcaaaaagt
      541 ttctcaactt tttgagtaat aagttcctgt tcctcaaaag gagctaacgg aattaatagc
      601 tcgcttagtg tagttttagg aatattatat aaagcttgac ctgatagttt agttattgct
      661 ttcaattgtt tataaaataa cggagaggac aagttaaata atagaaattt tgaaataatc
      721 tctgaacttt cgaatggtgt taattggaaa ataaatccac cagccacaac accatcatag
      781 tctttatcga ttcttgcaaa ctttccaata tgttctaaag aggttgatac aggtgttatt
      841 agctgattat gttttaaata aacttgctca gaggagatga attgtgtatc aatgtagtaa
      901 tcattatcca acagagaaaa ttctaaaggc ttaatattac caccacgtat aattctaaca
      961 cctttattat taatgcttaa atcgcccttc ttgtaagaaa gacctgtatt cattgaaaaa
     1021 atatctttta tttttataac aacccaattc ataggtat
//
LOCUS       DAVKJJ010000131         1014 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000131 DAVKJJ010000000
VERSION     DAVKJJ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.131"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            163..885
                     /locus_tag="VW144_002065"
     CDS             163..885
                     /locus_tag="VW144_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEU9601448.1"
                     /translation="MIPKKIHYCWFGGKPLSNDVKRCIASWKKFCPDYEIIEWTEKNF
                     CIENQNQFVQDAYRDKAWAFVSDYARLKIIYENGGIYLDTDVEVIKNLDKLLENKAFF
                     GVHQVNHLVNTGLGFGSEKGTSILKELLNLYDEIEFDLSKKDELLCPELNTPVFKRLG
                     YTYSDCVVKNEYFTIYPEEYFDPISLGDGVENILSDKTFSIHHYSASWTSLRNQMKSK
                     IIRKLGRSNILELKRILKGKTR"
BASE COUNT          358 a          115 c          184 g          357 t
ORIGIN      
        1 agatttgaaa aataaaatta tttttgctgc attcaattat aaagaactag ataatcttat
       61 tcattttacc gatcctagag ggaatggtaa tgtaggtaca gacttatttt attatgatca
      121 atattttgat gttgtaaatt ggctgaatgg agagacacaa acatgatacc taaaaagatt
      181 cattattgtt ggttcggtgg caaaccttta tcaaatgatg taaagcgatg tatagcaagt
      241 tggaaaaaat tttgtcctga ttatgaaatt attgaatgga ctgaaaaaaa cttttgtatt
      301 gaaaatcaaa atcaatttgt tcaggatgca tatagagata aagcatgggc ttttgtaagc
      361 gattatgcaa gacttaaaat tatatatgaa aatggaggta tttatttaga tactgatgtt
      421 gaagtgatta aaaatctaga taagttgttg gaaaataaag cattctttgg agttcatcaa
      481 gttaatcatc tagttaatac aggattaggg tttggatcgg aaaagggcac ctctatttta
      541 aaggagttat taaatttgta tgatgaaata gaatttgatc ttagtaagaa agatgaattg
      601 ttatgtcctg aattaaatac acctgtattt aaacgtcttg gttatactta ttctgactgt
      661 gttgtaaaaa acgaatattt cacaatctat cctgaagaat attttgatcc gatttctttg
      721 ggtgatggcg tagaaaatat tttatcagat aaaacattct ctattcatca ttatagtgct
      781 tcttggactt ccttaagaaa tcagatgaag agcaaaataa ttcgtaaact aggcagaagt
      841 aatatcttgg agttaaaaag aatattaaag gggaaaacta gatgagaata tcaccttcga
      901 agctgatatt tgcatttttt agcagctttt atattcttgt aatccttttc ccaagaggaa
      961 tttttctagg acattctact ttagcttctc gattatggat tatttacaat ggaa
//
LOCUS       DAVKJJ010000132         1000 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000132 DAVKJJ010000000
VERSION     DAVKJJ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.132"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            105..584
                     /locus_tag="VW144_002066"
     CDS             105..584
                     /locus_tag="VW144_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU9601449.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            678..749
                     /locus_tag="VW144_002067"
     tRNA            678..749
                     /locus_tag="VW144_002067"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:711..713,aa:Glu,seq:ttc)
BASE COUNT          380 a          140 c          228 g          252 t
ORIGIN      
        1 gcctctcttt tttgtgcagt ttaggagcta aagggaacag aatggagaaa atggaacaaa
       61 tgtgttttct aatctgttag actgtatcta gaaaggggaa aattatgatt aaagaattgt
      121 atgaagaagt ccaagggact gtgtataagt gtagaaatga atattacctt catttatggg
      181 aattgtcgga ttgggaccaa gaaggcatgc tctgcttaca tgaattgatt agtagagaag
      241 aaggactggt agacgatatt ccacgtttaa ggaaatattt caaaaccaag tttcgaaatc
      301 gaattttaga ctatatccgt aagcaggaaa gtcagaagcg tagatacgat aaagaaccct
      361 atgaagaagt gggtgagatc agtcatcgta taagtgaggg gggtctctgg ctagatgatt
      421 attatctctt tcatgaaaca ctaagagatt atagaaacaa acaaagtaaa gagaaacaag
      481 aagaactaga acgcgtctta agcaatgaac gatttcgagg gcgtcaaaga gtattaagag
      541 acttacgcat tgtgtttaag gagtttacta tccgtaccca ttagtaagtc atgcaaaaaa
      601 aatgaaaaaa attagaaaaa gtagttgaca aagtttgaaa aggctgtata atagtaagag
      661 ttgaaaataa caactcaggt ccgttggtca aggggttaag acaccgcctt ttcacggcgg
      721 taacacgggt tcgaatcccg tacggactat ggtatgttgc gtcaggacca cttgatgaaa
      781 aaaagtttaa aaaaacttaa aaatcttcaa aaaagtgttg acaagcgaaa gcagttgtga
      841 tatactaata tagttgtcgc ttgagagaag caagtgataa agacctttga aaactgaaca
      901 agacgaacca atgtgcaggg cgctacaaca taagttgtag tactgaacaa tgaaaaaaac
      961 aataaatctg tcagtgacag aaatgagtaa gaactcaaac
//
LOCUS       DAVKJJ010000133          948 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000133 DAVKJJ010000000
VERSION     DAVKJJ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.133"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            102..251
                     /locus_tag="VW144_002068"
     CDS             102..251
                     /locus_tag="VW144_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEU9601450.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVL"
     gene            294..659
                     /locus_tag="VW144_002069"
     CDS             294..659
                     /locus_tag="VW144_002069"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEU9601451.1"
                     /translation="MPNSESTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYYGDSAWEHLRAVTGI
                     DLLSILTDIANKKTGVKSK"
     gene            656..>948
                     /locus_tag="VW144_002070"
     CDS             656..>948
                     /locus_tag="VW144_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU9601452.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNI"
BASE COUNT          393 a          117 c          158 g          280 t
ORIGIN      
        1 caagaggata gaaatgaccg aattagaaag aaaaaatcga aaaattagct aagaaatact
       61 ctgataactt aaacatcaaa gttcaagaga gagttcgtga aatggcaaat gataataaga
      121 gccattattt gatatacaga gttttaggta tttcatttga agaaggagaa aatatcgatt
      181 tgtatcaaaa taaaggtcgt tttttataca aatatgctgg ttcattttta gaagaagctg
      241 cagtactatg atttaacgaa aaatttggta tagaaaatac ttaaaaagtt aacattccta
      301 attctgaaag tacaaaacct aagacttttg aaattgattg tttagtcgga gaaaaacacg
      361 catacgaaat aaaatggtgg gatgcaacta cagatggaga ccatataact aaagaacaca
      421 ctagaataaa agttattcat aacaaaggat atataccaat tcggttaatg ttctactatc
      481 caaatagaac tcaagccata aaaattcagc aaactttaga aacattgtat aacggtattg
      541 gagggaaata ttattatgga gattctgcct gggaacattt aagagcagtg accggtattg
      601 atttacttag tattctaaca gatattgcaa ataaaaaaac aggggtaaaa tcaaaatgac
      661 agtattaaaa ggagataact tagaaatatt aaaaactatt gaatcctcaa gtattgattt
      721 aatctatatg gaccctcctt tctttacaca gaaaacccaa aaattatcta ataacaaaaa
      781 tattatgtat tcattcgaag atacgtggac ttcgattgag gattacaaag aatttttgtc
      841 tgtaagatta gaagaatgca aaagagtgct aaaaaatagt ggcagtattt tcgttcattg
      901 tgataaaatt gcaaatcatc atattagatt aattttagat aatatctt
//
LOCUS       DAVKJJ010000134          941 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000134 DAVKJJ010000000
VERSION     DAVKJJ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.134"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            271..941
                     /locus_tag="VW144_002071"
                     /pseudo
     CDS             271..941
                     /locus_tag="VW144_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          323 a          161 c          189 g          268 t
ORIGIN      
        1 tttagttgat ttcatatcac tattatataa tgctttttga ttttagttta gtataaatag
       61 cgagaaatat ctatcctatc ttctagaatg tcttccaaac gaggaaactc tcgtaaacaa
      121 agaggtttta gaggcttatt taccatggac taaagttgta caagaaaagt gcaaataaga
      181 aatctcctga ttaggaacta tccgtgagtt ctctagtctg gagatttttc aatagacttc
      241 ctgcgaaaca aaatatggta taatagttct atgaattatg aagcaagtaa acaactaacc
      301 gatgcacgat ttaagcgtct tgttggtgtt caacgcacga cttttgaaga gatgttagct
      361 gtattaaaaa cagcttatca acttaaacac gcaaaaggtg ggcgaaaacc taaattaagc
      421 ctagaagacc ttcttatggc cactcttcaa tatgtgcgag aatatcgaac ttatgaagaa
      481 attgcggctg atcttggtat tcacgaaagc aacttaatcc gtcggagcca atgggttgaa
      541 gtaactcttg ttcaaaggtg gtgttgcgat ttcaagaact cctctcagtt ctgaggacac
      601 ggtaatgatt gatgcgacgg aagtaaaaat caatcgacct aaaaaaagaa ttagcgaatg
      661 attctggtaa aaagaaattt cacgctatga agactcaagc gattgtcaca agtcaaggga
      721 gaattatttc tttggatatc gctgtgaact attgtcatga tatgaagttg ttcaaaatga
      781 gtcgcagaaa tatcggacaa gctggtaaaa tcttggctga cagtggttat caagggctca
      841 tgaagatata tcctcaagca caaattccac gtaaatccag caaactcaag ccgctaatag
      901 ctgaagataa agcctataac catgcgctat ctaaggagag a
//
LOCUS       DAVKJJ010000135          914 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000135 DAVKJJ010000000
VERSION     DAVKJJ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.136"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            <1..707
                     /gene="galE"
                     /locus_tag="VW144_002072"
     CDS             <1..707
                     /gene="galE"
                     /locus_tag="VW144_002072"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9601453.1"
                     /translation="NILKLMKKYKVFNFVFSSSATVYESTPIMPFYETNPLKASNPYG
                     RTKQYIEVLLNDLFISNSNWKIVCLRYFNPLGAHESGDLGEDPNGIPNNLVPYITQVA
                     IGKLPYLNIFGVDYSTPDGTCIRDYVHVNDLAYGHRKALEYIFNTDEGLYEVINLGSG
                     VGFSVFEILHSLESVIGSYIPYKITSRRAGDMDVSIADISKAEELLGWKPRYDIMKMC
                     QDTWKWQQKHPNGYDD"
BASE COUNT          330 a          115 c          176 g          293 t
ORIGIN      
        1 taaatatttt aaaactaatg aaaaaatata aggtatttaa ttttgtgttt agttcatcag
       61 ctactgtata cgaaagtact cctatcatgc cattctatga gactaatcca ttaaaagctt
      121 caaacccgta tgggagaaca aaacagtata tagaagtact tttaaatgat ttgtttattt
      181 cgaattcaaa ttggaaaatt gtttgtctga ggtattttaa tccattgggt gcacatgaaa
      241 gtggtgattt aggagaggat ccaaatggaa ttccgaataa tcttgttcct tacattacac
      301 aagttgcaat tggaaaatta ccatatttaa atatattcgg agttgattac agtactcctg
      361 atgggacttg tataagggac tatgttcacg ttaacgattt agcatacgga cacagaaaag
      421 cattagagta tatttttaat acagatgaag gtttatatga agtaatcaat ttaggaagtg
      481 gtgttggttt tagtgtattt gaaatcctac acagcttaga gagtgtcata ggctcgtata
      541 ttccatataa gataacttca aggagagcag gagatatgga cgtttcaatt gcagatattt
      601 caaaagcaga agagctactg ggatggaagc caaggtatga tattatgaaa atgtgccaag
      661 atacgtggaa atggcaacag aaacatccaa atggatatga tgactaataa ttgaaaggat
      721 tgggattaac atgcatatag tattatttag atattctgca cgggttgtta aactaaaata
      781 tctgtataac agaaatgtag taattattgc acctgaggtt caaagaaaaa aatatgaaga
      841 ttatctagct gaaggaaata atctacctaa ggtcattttt ttagaagatt ttattctacc
      901 aaatatcatc ttag
//
LOCUS       DAVKJJ010000136          883 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000136 DAVKJJ010000000
VERSION     DAVKJJ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.137"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            1..865
                     /locus_tag="VW144_002073"
                     /pseudo
     CDS             1..865
                     /locus_tag="VW144_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
BASE COUNT          323 a           78 c          184 g          298 t
ORIGIN      
        1 actcaactaa ttatttttta tatatgttaa aaaaacttga caagaatgga tttggaaagg
       61 ttaagacagt gataacatgc catggatggg ttgaatataa cttaaaaaag aaattcttga
      121 cttattttga tttttggaca tattctatgg gagatgcttt tatttgtgta tctgaaacta
      181 tgaaaaaaag attggagagt ataataaaaa ataagaagat agttgcaata aataacggaa
      241 ttaatgtatc aaatagcgat ttagatgtag taggtgttca agactttaaa aaagaatttt
      301 gtattccaaa taacaaaaaa tcatttgtta tgttggaagg ttggatccag aaaaaaggcc
      361 ggatagattc cttgaatttg cagaaaaatt atttttagtc agagaagatg tgatttttat
      421 tatggctgga aatggtagta tgtgggctgc tttgaaagaa aaaatttgcc atttgaaatg
      481 tagagataat ttcaggttac ttggtgaaat ctatccagct actatagttt accaaatatc
      541 ggatttattg tatattcctt ctgatacaga aggaatacct atgtgtgttt tagaatcaat
      601 gtcacaagga actccggttt tagctagtaa tgttggcggg ttaagtgaaa ttattgaaca
      661 tagggttgat ggatttttat ttgagaagga agatgttgag ggagtgtgtg cttgtgctaa
      721 ttttttactc aatgattctg agtatttgaa atatataggt gagaatagta aatcaaaaat
      781 aagaaaacat ttttctgtgc aaaaaatgtt tgtagaaacc atgaaagtat atgatgaatt
      841 attagagaag agtagtcatg gataggaata tagatcaaga att
//
LOCUS       DAVKJJ010000137          881 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000137 DAVKJJ010000000
VERSION     DAVKJJ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.138"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(8..81)
                     /locus_tag="VW144_002074"
     tRNA            complement(8..81)
                     /locus_tag="VW144_002074"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45..47),aa:Ile,seq:cat)
     gene            complement(95..168)
                     /locus_tag="VW144_002075"
     tRNA            complement(95..168)
                     /locus_tag="VW144_002075"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(132..134),aa:Met,seq:cat)
     gene            complement(174..247)
                     /locus_tag="VW144_002076"
     tRNA            complement(174..247)
                     /locus_tag="VW144_002076"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(211..213),aa:Pro,seq:tgg)
     gene            complement(263..336)
                     /locus_tag="VW144_002077"
     tRNA            complement(263..336)
                     /locus_tag="VW144_002077"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(300..302),aa:Arg,seq:acg)
     gene            complement(346..431)
                     /locus_tag="VW144_002078"
     tRNA            complement(346..431)
                     /locus_tag="VW144_002078"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(395..397),aa:Leu,seq:taa)
     gene            complement(439..510)
                     /locus_tag="VW144_002079"
     tRNA            complement(439..510)
                     /locus_tag="VW144_002079"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(476..478),aa:Gly,seq:gcc)
     gene            complement(533..605)
                     /locus_tag="VW144_002080"
     tRNA            complement(533..605)
                     /locus_tag="VW144_002080"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(570..572),aa:Thr,seq:tgt)
     gene            complement(624..705)
                     /locus_tag="VW144_002081"
     tRNA            complement(624..705)
                     /locus_tag="VW144_002081"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(669..671),aa:Leu,seq:tag)
     gene            complement(711..783)
                     /locus_tag="VW144_002082"
     tRNA            complement(711..783)
                     /locus_tag="VW144_002082"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Lys,seq:ttt)
     gene            complement(808..880)
                     /locus_tag="VW144_002083"
     tRNA            complement(808..880)
                     /locus_tag="VW144_002083"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(845..847),aa:Asp,seq:gtc)
BASE COUNT          232 a          257 c          199 g          193 t
ORIGIN      
        1 attcaaatgg accttgtagg acttgaacct acgaccactc ggttatgagc cgagagctct
       61 aaccagctga gctaaaggtc cgacaagatc attatagcgg cgaaggggat cgaacccccg
      121 acctcccggg tatgaaccgg acgctctagc cagctgagct acaccgccat gaatcgggaa
      181 gacaggattc gaacctgcga caccttggtc ccaaaccaag tactctacca agctgagcta
      241 cttcccgagt taaatagaaa aatgcaccct agaggagtcg aacctctaac cgcctgattc
      301 gtagtcaggt actctatcca gttgagctaa gggtgctcca tattatgccg aggaccggaa
      361 tcgaaccggt acgatcgtta ccaatcgcag gattttaagt cctgtgcgtc tgccagttcc
      421 gccaccccgg cctctctaag cgaacgacgg gattcgaacc cgcgaccccc accttggcaa
      481 ggtggtgttc taccactgaa ctacgttcgc actgttttct tctatctaaa aatgccggct
      541 acatgacttg aacacgcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      601 ccggctcatt tgttatatct taatgcgggt taagggactt gaacccccac gccgttaagc
      661 gccagatcct aaatctggtg cgtctgccaa ttccgccaaa cccgcatata tgacccgtac
      721 tgggctcgaa ccagtgaccc attgattaaa agtcaattgc tctaccaact gagctaacga
      781 gtctaaaata acttgcgtta ccttaaacgg tcccgacggg aatcgaaccc gcgatcttcg
      841 ccgtgacagg gcgacgtgat aaccgctaca ctacgggacc t
//
LOCUS       DAVKJJ010000138          830 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000138 DAVKJJ010000000
VERSION     DAVKJJ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.139"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            21..830
                     /locus_tag="VW144_002084"
                     /pseudo
     CDS             21..830
                     /locus_tag="VW144_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS1515 family transposase"
BASE COUNT          267 a          174 c          161 g          228 t
ORIGIN      
        1 aaaagtgtta tactattttt atggaaacag tatacgacaa agcacaaaaa cttaactcaa
       61 aaaacttcaa actattgatt ggtgtcaaaa aggaaacctt tcaactcatg ctagaacacc
      121 tgaattcagc ctatcagatt cagcaccgaa aaggtggacg tccacgtagt ctgcccatgg
      181 aagaccagct cattatgacc ctccgttact tgcgatatta tcccactcag cgtctgctgg
      241 cctttgattt tggcgtcggt gtagctacgg taaatgccat catcacttgg gtggaggata
      301 cacttcgtgc gtcaggtagc tttgatttgg accatttaga agccccgagt gctgctgtgg
      361 ctattgacgt gaccgaaagt ccgattcagc gtccaaaaaa aaccaaagca aaaattattc
      421 tggtaaaaag aaacgacaca ccttaaaaac tcaaattatg ctggatttga cgacacataa
      481 agtctgtcaa atggcctttt ctgacggaca tacgcatgat tttactctct tcaaagaaag
      541 tattggacaa agtttgcctg aaacgacgct tgcctttgtt gacctaggtt atttaggcat
      601 cttgaaattt catgagaata ctttcattcc tgctaaaaat tccaaaaatc gccgcctgag
      661 tgaggatgat aagcagttaa ataaagagat gtcagcgata cgaattgaaa ttgaacattt
      721 taacgctaaa ttcaaaacct tccaaatcat gtcagtccct tatcgtaacc gcagaaaacg
      781 tttcgagtta cgggcggaat taatttgtgc catcatcaat tatgaagtga
//
LOCUS       DAVKJJ010000139          780 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000139 DAVKJJ010000000
VERSION     DAVKJJ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.140"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(143..214)
                     /locus_tag="VW144_002085"
     CDS             complement(143..214)
                     /locus_tag="VW144_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHP2/SHP3 family peptide pheromone"
                     /protein_id="HEU9601454.1"
                     /translation="MKKISKFFPILMLVMDIIIIVGG"
     gene            296..>780
                     /locus_tag="VW144_002086"
     CDS             296..>780
                     /locus_tag="VW144_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="HEU9601455.1"
                     /translation="MKSKLGITLRKVRKGKQISLCSVADEHLSKSQISRFERGESEIS
                     CIRLINILDKLHITLDEFLILHDEDYTKTESFANLIQYIRKQYSLQNINNIQSLLSDS
                     SNYTLDSFEKTMVKSILHTMDSRIIPSDDELLQLADYLFKVEKWGYYEIILLGNCVRT
                     I"
BASE COUNT          285 a          127 c          101 g          267 t
ORIGIN      
        1 tataccatac cctataatta atactttttt atgtttcaaa gtctcaccac tctctaaaat
       61 tttacgattt aacttaaata tgttttagct agtaagtata gtctttagat actctaaaga
      121 cttaggtgta cacctaagtg atttaaccac caacaataat gataatgtcc atcactagca
      181 tcaaaattgg gaaaaatttt gaaattttct tcatatttag acttcctttc aatttttatt
      241 tattgtacaa taaataaaac taagttaact ttttgttggg agaatgggaa aaactatgaa
      301 atcaaaactt ggcatcacac ttagaaaagt tcgtaaaggc aaacaaatca gtctatgctc
      361 tgttgctgat gagcatcttt caaaatcgca aatatcccga tttgaacgtg gagagtcaga
      421 gatatcatgt atccgactga tcaatatttt agacaaatta cacattactt tggatgagtt
      481 tcttatttta cacgatgaag actatacaaa aactgagtca tttgccaact taatacagta
      541 tatccgtaaa caatattcat tacaaaacat caataatata caatctctac tttcggattc
      601 ctcaaattac actttagatt cttttgaaaa aacaatggta aaatccattt tacacacaat
      661 ggattcaaga atcattccat cagatgatga attacttcaa ctagcagatt atctctttaa
      721 agttgaaaaa tggggttatt atgagattat tttattaggt aactgtgtac gaacaattaa
//
LOCUS       DAVKJJ010000140          769 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000140 DAVKJJ010000000
VERSION     DAVKJJ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.141"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            complement(<1..>769)
                     /locus_tag="VW144_002087"
     CDS             complement(<1..>769)
                     /locus_tag="VW144_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HEU9601456.1"
                     /translation="LSRTKNELSGVKSAQATYEETTTRRLSELTNLANGKASKSELTQ
                     TAEELASRIASVQAGSSRNYFRNSRSRTFTTGGQAVYDYRTFIVPDFWKNSDRFKRDY
                     VRISFDVTFPVALVNDMPAMVHFSAHPWYAYRNLIFKGGTVERQHFEFTIDLSSSSED
                     YQTNNVFIRFGTNYGFPAGLQVVIENAMLSVGNYFPAYQPAYEDQEDRVSVVESNFKQ
                     RADSLDAGVSRLTEGLRTKADISSLNVTAENIRQSVKR"
BASE COUNT          212 a          186 c          161 g          210 t
ORIGIN      
        1 cctcttcaca gattgccgga tattttcagc agtcacattg agtgagctga tatcggcttt
       61 agttctaagg ccttcagtca gacggcttac accagcgtcg agtgaatcag cacgctgctt
      121 aaagttggat tcgactactg agacacggtc ttcttggtct tcatacgctg gttgataggc
      181 tggaaaataa ttaccaaccg ataacatagc gttctcaatg acgacctgca gaccagcagg
      241 aaatccataa ttagtaccaa aacgaatgaa cacattatta gtctgatagt cctcagaaga
      301 actagacaag tcaatcgtaa actcaaaatg ttggcgttcg acagttccac ctttaaaaat
      361 taagtttctg taggcatacc atggatgagc actaaaatgc accatagcag gcatgtcatt
      421 tactagggcg acagggaaag tcacatcaaa agatatgcga acataatcac gcttgaacct
      481 gtcactgttc ttccagaaat caggaactat gaatgttcga tagtcgtata ccgcttgacc
      541 tcctgttgtg aacgttcttg aacgtgaatt cctgaagtaa ttccgtgaac tacctgcctg
      601 cacactcgca atccgactag ccagctcctc agctgtttgc gtgagttctg acttgctggc
      661 tttaccattg gccaagttgg tcagttctga cagtctacga gtcgtcgtct cctcatacgt
      721 cgcttgcgct gacttcacgc cagacagttc attcttagtc cgactaagt
//
LOCUS       DAVKJJ010000141          663 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000141 DAVKJJ010000000
VERSION     DAVKJJ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.142"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            <1..>663
                     /gene="phtD"
                     /locus_tag="VW144_002088"
     CDS             <1..>663
                     /gene="phtD"
                     /locus_tag="VW144_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HEU9601457.1"
                     /translation="PQPAPNPQPAPSNPIDEKLVKEAVRKVGDGYVFEENGVSRYIPA
                     KDLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFYNKAYDLLARIHQDLLDNK
                     GRQVDFEALDNLLERLKDVSSDKVKLVDDILAFLAPIRHPERLGKPNAQITYTDDEIQ
                     VAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAK
                     EK"
BASE COUNT          217 a          131 c          149 g          166 t
ORIGIN      
        1 gtccgcaacc tgcaccaaat cctcaaccag ctccaagcaa tccaattgat gagaaattgg
       61 tcaaagaagc tgttcgaaaa gtaggcgatg gttatgtctt tgaggagaat ggagtttctc
      121 gttatatccc agccaaggat ctttcagcag aaacagcagc aggcattgat agcaaactgg
      181 ccaagcagga aagtttatct cataagctag gagctaagaa aactgacctc ccatctagtg
      241 atcgagaatt ttacaataag gcttatgact tactagcaag aattcaccaa gatttacttg
      301 ataataaagg tcgacaagtt gattttgagg ctttggataa cctgttggaa cgactcaagg
      361 atgtctcaag tgataaagtc aagttagtgg atgatattct tgccttctta gctccgattc
      421 gtcatccaga acgtttagga aaaccaaatg cgcaaattac ctacactgat gatgagattc
      481 aagtagccaa gttggcaggc aagtacacaa cagaagacgg ttatatcttt gatcctcgtg
      541 atataaccag tgatgagggg gatgcctatg taactccaca tatgacccat agccactgga
      601 ttaaaaaaga tagtttgtct gaagctgaga gagcggcagc ccaggcttat gctaaagaga
      661 aag
//
LOCUS       DAVKJJ010000142          630 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000142 DAVKJJ010000000
VERSION     DAVKJJ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.143"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            <1..>630
                     /locus_tag="VW144_002089"
     CDS             <1..>630
                     /locus_tag="VW144_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9601458.1"
                     /translation="SKLKVPYLKEGGEYQIKNLSYKFTDDECLSLKDISFKLGKIYGI
                     IGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVMQDVNHQLFTDEV
                     FNELRLGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAIASVMCKNSPFIF
                     FDEPSSGMDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADEIFE"
BASE COUNT          248 a           71 c          117 g          194 t
ORIGIN      
        1 aagtaaatta aaagttcctt atttaaaaga aggtggagag tatcagataa aaaatcttag
       61 ctacaaattt actgatgatg agtgtttaag cttaaaagat atttcattca agcttgggaa
      121 aatttatggc ataataggat ccaacggacg aggaaaatca acgcttttaa gatgtttaat
      181 aggtcttgag aaaaaatcaa aagaagaaat ttattttaag ggagagaagc tatctaaaaa
      241 agaaagactc aaaaactctt cacttgttat gcaagatgta aatcatcaat tattcacaga
      301 tgaagtattc aacgagctta gattaggagt aaagaatttt gatgaagaaa aggcgaaaat
      361 cattttaaaa gatttaggcc tggacgaatt tattgaaagg catccgatga gtttatcagg
      421 agggcaaaag caaaggcttg caatagcatc tgttatgtgc aagaattctc catttatctt
      481 ttttgacgaa ccttcaagtg gtatggatta ttccaatatg ataaaaatat ctgaactgat
      541 taataagtat aaaaccatgg ataaaataat ttttattgtt tcccatgata tagaattttt
      601 aaatgaagtg gcagatgaaa tttttgaatt
//
LOCUS       DAVKJJ010000143          602 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000143 DAVKJJ010000000
VERSION     DAVKJJ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.144"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            <1..>602
                     /locus_tag="VW144_002090"
     CDS             <1..>602
                     /locus_tag="VW144_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEU9601459.1"
                     /translation="RIIFGLYCFYLLFFSGRRQLKIEKKYIKYGLSYSLPLIPHFLSH
                     YILNQSDRLMINMFDGKEKLAIYSLAYSVSMIMFLFTNAINQSIMPHTFQALKKKDYC
                     GIHGSTKWLFVVVGGITALSILFAPELIVILGGNKYKESIWLVPPIAISVYFLFVYSM
                     FSNISFYYKMNKLISLVSTGAALSNIILNYIFINIFGYQA"
BASE COUNT          178 a           80 c          101 g          243 t
ORIGIN      
        1 tccgaattat ctttggttta tattgttttt atctcttgtt tttttcaggg agaaggcaac
       61 ttaagataga aaaaaaatat attaaatatg gtttgagtta ttcattacct ttgataccgc
      121 atttcttatc gcactatatt ttgaatcagt ctgaccgttt gatgattaac atgtttgatg
      181 gtaaggagaa attagctata tatagtctag cttattctgt atcaatgatt atgtttttgt
      241 ttactaatgc aattaatcaa tcaataatgc cgcatacatt tcaagctttg aaaaaaaagg
      301 attattgtgg gatacatggg agcaccaaat ggctttttgt agtagttggg gggattacag
      361 ctctctcaat tctatttgca ccagaactaa ttgttatttt aggcggcaat aaatataagg
      421 aatcaatttg gctagtacct ccaattgcca tctcggttta tttccttttt gtatattcta
      481 tgtttagtaa tatcagtttt tattataaga tgaataagct gatttcgtta gtttccacag
      541 gtgcggctct ttcaaatatt attttaaatt atatttttat taatatattt gggtatcaag
      601 ct
//
LOCUS       DAVKJJ010000144          583 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000144 DAVKJJ010000000
VERSION     DAVKJJ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.145"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            <1..>583
                     /locus_tag="VW144_002091"
     CDS             <1..>583
                     /locus_tag="VW144_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9601460.1"
                     /translation="RNKIIHQFGYTNHRIKLILSNEDLGATNARNIGIKNSRGKYISF
                     LDDDDEYMPDRILKLMACVKKSRMKNLALVYSYGIIIYPNGTREEEKTDFVGNPLFVQ
                     MVHNIAGTSFWLCKKEVLELINGFEKIDSHQDGVVLLKLLAQGYQIDIVREFLVNYYA
                     HSKENGITGVTQKTINADEEYYNYCRKYFNLLS"
BASE COUNT          219 a           62 c          113 g          189 t
ORIGIN      
        1 taagaaataa aattatccat caatttggct ataccaatca tagaattaag ttaattttaa
       61 gtaatgaaga tttaggtgca actaatgcca gaaacatagg tatcaaaaat tctagaggta
      121 agtatatatc atttttagac gatgatgatg aatatatgcc agatcgaatt ttaaagttga
      181 tggcttgtgt taaaaagagt agaatgaaga atttagcttt agtttatagt tatggaataa
      241 taatttatcc aaatggtaca cgagaagagg agaagaccga ttttgttgga aatcccttgt
      301 ttgttcaaat ggttcacaat atagcaggta cgtcattttg gttgtgtaaa aaagaggtgc
      361 tagaattaat taatggtttt gagaaaatag attcacatca ggacggtgtt gttttattaa
      421 aactacttgc tcaaggatac caaattgata tagtgcgaga attcttggtg aattactacg
      481 ctcacagtaa agaaaacggt atcactggag tgacacaaaa aacaattaat gcagatgaag
      541 aatattataa ttactgtagg aaatatttta atttattgag ttt
//
LOCUS       DAVKJJ010000145          570 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000145 DAVKJJ010000000
VERSION     DAVKJJ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.146"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            1..570
                     /locus_tag="VW144_002092"
                     /pseudo
     CDS             1..570
                     /locus_tag="VW144_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          195 a          111 c          121 g          143 t
ORIGIN      
        1 cagcttatca acttaaacac gcaaaaggtg gacgaaaacc taaattaagc ctagaagacc
       61 ttcttatggc cactcttcaa tatgtgcgag aatatcgaac ttatgaacaa attgcggctg
      121 attttggtat tcacgaaagc aacttaatcc gtcggagcca atgggttgaa gtaactcttg
      181 ttcaaagtgg tgttacgatt tcaagaactc ctctcagttc tgaggacacg gtaatgattg
      241 atgcgacgga agtacaaatc aatcgcccta aaaaaagaat tagcgaatca ttctggtaaa
      301 aagaaatttc acgctatgaa ggctcaagcg attgtcacaa gtcaagggag aattgtttct
      361 ttggatatcg ctgtgaacta tagtcatgat atgaagttgt tcaaaatgag ttgcagaaat
      421 atcggacaag ctggtaaaat cttggccgac agtggttatc aagggctcat gaagatatat
      481 cctcaagcac aaactccacg taaatccagc aaactcaagc cgctaacagc tgaagataaa
      541 gcctgtaacc atgcgctatc taaggagaga
//
LOCUS       DAVKJJ010000146          552 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000146 DAVKJJ010000000
VERSION     DAVKJJ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.147"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            <1..100
                     /locus_tag="VW144_002093"
     CDS             <1..100
                     /locus_tag="VW144_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HEU9601461.1"
                     /translation="QVDETDEVDGMRFSTFSTNRCGNWSAFSWENC"
BASE COUNT          158 a           67 c          126 g          201 t
ORIGIN      
        1 tcaagttgac gaaacagatg aagttgatgg aatgcgattt agtacattca gtacaaaccg
       61 ttgcggtaac tggagtgctt ttagttggga aaattgttaa ttcatttaaa ttgtaaactt
      121 tagagattct ctggagttat ggatgtcgtt ccaatatgtg cacgttggaa tgttagtgct
      181 tatatggggg gaacagaatc ctctcttgat tgaagacaag ctagtcatta ggctggtttg
      241 tctttttgtc aactgtagtg ggttgatata atagtattag tgagtgggat aaaagtttca
      301 tttagtttat tcagtacaaa tttaacgggt caagatttat atactagtgg tgtttttggg
      361 gctgagagaa gtatcttgat tttatgtgtg gtttttatac ttacagttgt tctgctccaa
      421 agagcttgta gagaagaatt agctcataaa ggagattgat tattttgata tcaaaaaaat
      481 gcacaggata acctgatgca tttttttagc gacaatgctt gctacttcct tctgtcgaat
      541 ttagacaatt tt
//
LOCUS       DAVKJJ010000147          477 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000147 DAVKJJ010000000
VERSION     DAVKJJ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.148"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
BASE COUNT          165 a           62 c           86 g          164 t
ORIGIN      
        1 gttgatactg taatgagcca agatttagaa tattttcccg atatagtatg gagattggaa
       61 ggaagtctct ttgaatgtgg tgtcatactg cagtactatt taggcaggtt gttgttccaa
      121 aaagggaaca caagtatttt ttgtggcgag tcagcggatg aaataatgac ttttaagtat
      181 cattctgtga actacaatca attttgtaat gataaacaga agtcagtata ttttagttat
      241 tccgactatc cattttatgt tacaaattca attgttttaa agaaaaataa cctacttttg
      301 catagttttg gcattcatcc tagatatcca tataaaatgt ctgagattgt tgaaatgtct
      361 aaaaaaatat caaatctaaa cgataaaaaa gaatttcaca aaaagaactg tgagttgagg
      421 tttcaagata gtgttcttga taacataaat agtgtcccgg gtacaactca tttattt
//
LOCUS       DAVKJJ010000148          406 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074757
            SAMN33606202-rid17954173.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVKJJ010000148 DAVKJJ010000000
VERSION     DAVKJJ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606202
            Sequence Read Archive: SRR23727430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722472.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722472.1
            Genome Coverage       :: 279x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:00:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,094
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 1,941
            CDSs (with protein)               :: 1,941
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 61 of 108
            Pseudo Genes (incomplete)         :: 44 of 108
            Pseudo Genes (internal stop)      :: 28 of 108
            Pseudo Genes (multiple problems)  :: 21 of 108
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606202-rid17954173.denovo.150"
                     /strain="AUSMDU00074757"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST62"
     gene            184..406
                     /locus_tag="VW144_002094"
                     /pseudo
     CDS             184..406
                     /locus_tag="VW144_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin class II family protein"
BASE COUNT          152 a           47 c           99 g          108 t
ORIGIN      
        1 gtaaaaaagt gattagaaaa catctttttt aaaaatagag atgattttga aacaaaaaag
       61 ctaattcaag acgtttcgat gccaattcaa gatttggatg aaaaaaatta atagatactg
      121 ttatactaaa cttgtcaagt ttgtaacaag acaaatatta aaaataaaaa agaggtattc
      181 gttatgaata caaaaaagat gtcacaattt gaaattatgg atactgagat gcttgcttga
      241 gttgaaggtg gcggatgcaa ttggggagat tttgccaaag caggtgttgg aggaggagta
      301 gcacgaggtc ttcagctagg aattaaaaca agaacatggc aaggtgcagc aactggtgct
      361 gcgggaggag ctatacttgg aggtgtggcc tatgaagcga catgtt
//
